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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV47</accession>
    <name>W5LV47_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamine amidotransferase-like class 1 domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ferry endosomal RAB5 effector complex subunit 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Parkinson disease 7 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FERRY complex (Five-subunit Endosomal Rab5 and RNA/ribosome intermediary). The FERRY complex directly interacts with mRNAs and RAB5A, and functions as a RAB5A effector involved in the localization and the distribution of specific mRNAs most likely by mediating their endosomal transport. The complex recruits mRNAs and ribosomes to early endosomes through direct mRNA-interaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="FunCoup" id="W5LV47">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000004"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000004.1">
        <property type="protein sequence ID" value="ENSLOCP00000000004.1"/>
        <property type="gene ID" value="ENSLOCG00000000004.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS6W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165047"/>
    <dbReference type="HOGENOM" id="CLU_098485_0_0_1"/>
    <dbReference type="InParanoid" id="W5LV47"/>
    <dbReference type="OMA" id="QKKPVCA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000004">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03141">
        <property type="entry name" value="GATase1_Hsp31_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF18">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE-LIKE CLASS 1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="215" mass="22569" checksum="CE121D3F618277BE" modified="2014-04-16" version="1">EMSSKPTCLIVTSAAPEGVSAQSFLQAFSLCSSAFNVQTATPGGKAIDFVGVDETSARWVQDFSVKPYANPAKLESIDGARYHALLIPDCPGALTDLAHSGSLARILTHFXVPAEPVCAVGQGVAALCCATEGQAWIFRGYSLTGPSVCELVRRLDFASLPLIVEDFAKDCGAAYAASQEDAVHIVLDRHLITGQNSQSTAAAVNNLILLCGSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV82</accession>
    <name>W5LV82_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LV82"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000039"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000039.1">
        <property type="protein sequence ID" value="ENSLOCP00000000039.1"/>
        <property type="gene ID" value="ENSLOCG00000000034.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1739908_0_0_1"/>
    <dbReference type="InParanoid" id="W5LV82"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000034">
        <property type="expression patterns" value="Expressed in mesonephros and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF303">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER INE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17302" checksum="C22D188AA4856166" modified="2014-04-16" version="1">MGVGTENRGLDFTLFGQNGYSYEEEQRPLRTYIEVKGVEMRDQAVRTDERGTWSRRLEFVLASVGYAVGLGNVWRFPYLCYRSGGETSGHTCRLLVTVSDCSGDEKKSERRKRGAFHILLLDTRWILPKYFVLMRTVLEGVSTWINVMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVH5</accession>
    <name>W5LVH5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVH5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000132"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000132.1">
        <property type="protein sequence ID" value="ENSLOCP00000000132.1"/>
        <property type="gene ID" value="ENSLOCG00000000118.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SI5J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161369"/>
    <dbReference type="HOGENOM" id="CLU_012526_1_3_1"/>
    <dbReference type="InParanoid" id="W5LVH5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="335" mass="38609" checksum="A6196BE7AFA0609C" modified="2014-04-16" version="1">FTMSDTNQSSIKEFIIVGFPGLQPQYHSLVALVFFLVYVTILIGNILTTALLFLERSLQKPMYYIILNLALSDICFSTVTLPKIIVRYWFQLKSISFVDCFVQKQLVHYFGTLNSFIMMLMALDRYVAICFPLSYHMLITNRTILILSCMLWLISWIFPTVTTIHQLALYYCGPNTINQCYCESNSITKLACDDPTSSMMVSFALSMFVLLVPLSFTIFSYSQIIISVLRIANSQSRYKAFSTCSAQLGIIALYYVPRCVVYVTNTLRIIIRIDFHILLVLLYSLLPPMLNPLIYVIRTTEIKQCFIKRFRENTNSITELKLSFKTANRLQVIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVT0</accession>
    <name>W5LVT0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CTCK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVT0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000237.1">
        <property type="protein sequence ID" value="ENSLOCP00000000237.1"/>
        <property type="gene ID" value="ENSLOCG00000000214.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163235"/>
    <dbReference type="HOGENOM" id="CLU_1293988_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVT0"/>
    <dbReference type="OMA" id="PANDKEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000214">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF371">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="CTCK" evidence="6">
        <location>
            <begin position="91"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="112"/>
            <end position="161"/>
        </location>
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        <location>
            <begin position="123"/>
            <end position="177"/>
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            <begin position="127"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="23917" checksum="B2650E4EEE32F5BA" modified="2014-04-16" version="1">MMRAELKYMFRKRDGISNVKFYCTTLMTSPSRSQPGNTWSPPGDKCVKYECVKIEDQFVPVEAKITCPLFNRKDCIPGTETIAPDGCCHVCPLIPRNCNIKTNTTYITNNGCQSSKPVDITSCGGSCGTYSMYSVEANAMDHTCSCCQEMDTTQKQVQMVCPDGTTFSHSYTYVEKCGCKEIECEDRDTQQSTSKGQEPSKSSKGRKVYRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX32</accession>
    <name>W5LX32_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LX32"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000689"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000692.1">
        <property type="protein sequence ID" value="ENSLOCP00000000689.1"/>
        <property type="gene ID" value="ENSLOCG00000000623.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154869"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LX32"/>
    <dbReference type="OMA" id="HAEDEFP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000623">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865248" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="130" mass="14151" checksum="F93BDF24B6DCE39D" modified="2014-04-16" version="1">FMATEPTATMTFITIFLWTLLICIPESSGQVVMTQTPPALSVLSGGSVTIHCKASSSITGYYNYLNWYQQRPGEAPKLLIYWATVRQSGVPERFTGRGSGLDYSLTISGVQAEDAADYYCGQGAHTPLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXS1</accession>
    <name>W5LXS1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Troponin I4b, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LXS1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000928"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000932.1">
        <property type="protein sequence ID" value="ENSLOCP00000000928.1"/>
        <property type="gene ID" value="ENSLOCG00000000803.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="InParanoid" id="W5LXS1"/>
    <dbReference type="OMA" id="PLQMSGM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000803">
        <property type="expression patterns" value="Expressed in larva and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000008">
        <property type="entry name" value="Troponin I4b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF12">
        <property type="entry name" value="TROPONIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866975" evidence="6">
        <location>
            <begin position="24"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="194" mass="22520" checksum="B22CDA534B71298D" modified="2014-04-16" version="1">MHINSFFLVLFIFQLWNRGQKKAKIPASRRLFLKTKMLEKAITMIDKEKKDRETERERILAERVPPLTLSGLSVQELQDLCKQLHQKIDGIDEERYDIEVKVAKNDKEIEGLSQKILDLKGKFKRPNLRRVRISADAMLGALLGSKHKGSMDLRANLKSVKKEEETKDKEVTDWRKNVEAMSGMEGRKKLFDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXX2</accession>
    <name>W5LXX2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor C subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Activator 1 subunit 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. The RFC4 subunit probably functions as a scaffold on which the other complex components can assemble.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA. Forms an heterotetrameric complex with RFC2, RFC3 and RFC5; this complex has ATPase activity but is not stimulated by PCNA. The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17. Interacts with ATAD5. Interacts with CTF18. Interacts with CNTD1; this interaction facilitates crossover formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637459.1">
        <property type="nucleotide sequence ID" value="XM_006637396.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXX2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000979"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000983.1">
        <property type="protein sequence ID" value="ENSLOCP00000000979.1"/>
        <property type="gene ID" value="ENSLOCG00000000876.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683199"/>
    <dbReference type="KEGG" id="loc:102683199"/>
    <dbReference type="CTD" id="5984"/>
    <dbReference type="eggNOG" id="KOG0989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074917"/>
    <dbReference type="HOGENOM" id="CLU_042324_1_1_1"/>
    <dbReference type="InParanoid" id="W5LXX2"/>
    <dbReference type="OMA" id="GCQSGSF"/>
    <dbReference type="OrthoDB" id="10249205at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000876">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000010">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="359" mass="39822" checksum="3140127DF51B938F" modified="2014-04-16" version="1">MQAFLKGTPSLGPKPQKSKPTAGPSGEKRQKPVPWVEKYRPKCVDDVAFQEEVVAVLKRSLEGADLPNLLFYGPPGTGKTSTILAAARELYGPELFRQRVLELNASDERGIQVIREKVKNFAQLTVAGTRTDGKMCPPFKIIILDEADSMTGAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQEERLLEICEKENLKCTQEGIAALVQVSEGDLRKAITFLQSAARLTAGKEITESIVTEIAGVVPCKMIDSLLQTCYNSTFEKMESSVKDLIDQGFAATQILNQLHDSVFEDERLNDKQKSAIAEKMAEVDKCLADGADEFLQMIALCSVIMQQSSQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYJ2</accession>
    <name>W5LYJ2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Suppressor of tumorigenicity 14 protein homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LYJ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001199"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001203.1">
        <property type="protein sequence ID" value="ENSLOCP00000001199.1"/>
        <property type="gene ID" value="ENSLOCG00000001070.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107076243"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155418"/>
    <dbReference type="HOGENOM" id="CLU_688007_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYJ2"/>
    <dbReference type="OMA" id="CEPFASF"/>
    <dbReference type="OrthoDB" id="6380398at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001070">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF51">
        <property type="entry name" value="CUBILIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="6">
        <location>
            <begin position="80"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="213"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="335"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="41390" checksum="C515EB1644967BD5" modified="2014-04-16" version="1">MSSAVRFQSEARNGGHEQVTFLTNKEKMGSRKLTGVVLGMLAAVLVLSAVTGVLVWYFLVRPSGGDSSGGATGSPPRTDAVRVFSGHMVLSNLAYSQQLENADDPAFTQLADELQQMLKETYSKDDLLSKYYTKSVISAFSNGSVIAYHWTQFDIPASALESLPDFSEKRILEVLRAGIRQAGKSRKQEIVISSITSSETDPRMVRNPRGEECFHMLVAGDKVQTFQSPGYPDMYPPKVRCQWQIRAPQGTAIHLSFPVFHVEDDCKNDFVFVYDSLSPDESQAITKQCGQRPPSNTLEVISSGNIMLVNLISDGDTQRPGFTAEYQRVPLTTVCSEVLKNQNGNISSPHYPSFYPPSLDCKWTIEVQQGLLTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYP4</accession>
    <name>W5LYP4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LYP4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001251"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001255.1">
        <property type="protein sequence ID" value="ENSLOCP00000001251.1"/>
        <property type="gene ID" value="ENSLOCG00000001113.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000059940"/>
    <dbReference type="HOGENOM" id="CLU_745886_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYP4"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001113">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46440:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46440">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="175"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="39913" checksum="364A55DA3FBBB4B0" modified="2014-04-16" version="1">MEEWNSLPNYSSAYHTYAYGMTYPPPPVQNHPSLGWSGTGFTPSGSDGLFLDVPPTAPQNALPQDATRVPGVPLAGSPSDSSSETEPSTPDSWSSPSTDESYALRPPPAPCSPAETACAEPAPCSPAETACAEPAPCSPAEIAWAEPASSSQAEGVGEQGALLEPGPALAAGKRSHRLRMAYSERQVMVLCECYMIKMYLTPAEMKSLADKIGLSYKQVKSWFQNRRMREKRLQKKMTSGGELVLSSVYPSIQAQASKGPSSVYPKISPQTSESLSSVYPDISPQMLTDPTCPYSSIPHQPLESSSSVYPNISPQVLVNPSCCYPSIPPRISESPSSAYPYIPPQVSPSSVFLDESAWLCDFCVWMRESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZH4</accession>
    <name>W5LZH4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Poly(A) binding protein interacting protein 2B</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAIP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZH4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001531"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001536.1">
        <property type="protein sequence ID" value="ENSLOCP00000001531.1"/>
        <property type="gene ID" value="ENSLOCG00000001347.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S63Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017284"/>
    <dbReference type="HOGENOM" id="CLU_134152_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZH4"/>
    <dbReference type="OMA" id="EDKEWFI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001347">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040396">
        <property type="entry name" value="PAIP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154:SF5">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="132" mass="14859" checksum="9B96516CC75DC5D7" modified="2014-04-16" version="1">PTEPAEMNGPEVAKTPGGGSAPGKEEPVTNGHRESEPNPFAEYMWMENEEEYNRQVEEELLEQEFLERCFQEMLDEEDQDWFIPARDLPSGVGQLQQQLNGLSVADGSTTEDIVRKSSLNPEAKEFVPGVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZN0</accession>
    <name>W5LZN0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Conserved oligomeric Golgi complex subunit 8</fullName>
            <shortName evidence="9">COG complex subunit 8</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZN0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001587"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001592.1">
        <property type="protein sequence ID" value="ENSLOCP00000001587.1"/>
        <property type="gene ID" value="ENSLOCG00000001398.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015893"/>
    <dbReference type="HOGENOM" id="CLU_017968_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZN0"/>
    <dbReference type="OMA" id="QRCIHGV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001398">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016632">
        <property type="entry name" value="COG8_Metazoal_Plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015415">
        <property type="entry name" value="COG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="568"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="617" mass="68633" checksum="810CF9CF99D2DAF9" modified="2014-04-16" version="1">FYSTFIYAKLGEMTAEVDLTKMAAVDVEDESILAAIFKDSFPDNWRENPDFAAYLSELSSFGVDKLTREPERLAEERAQILQQTRELAFCNYKTFIRTADCTQEIYRDFGRVESSVSRLLDKLPGFGEKCRAFMTEAEDIGASRRMNSLTLNRHTEILEILEIPQLMDTCVRNGYYEEALELAAYVRRLEKKHCCLPVVQGIVHEVRQSAQLMLNQLLQQLRSNAQLPVCLRVIGYLRRMDVFTEAELRVKFLQTRGSWLGSILSAIPDGDPYFHITKTIEACRVHLFDIITQYRAIFSDEDPLLAPGGQAVNEGAIFHGWVVQKVSEFLETLERDLQRGVGSRLDSLLGQCMYFGLSFSRVGADFRGQLAPMFQRVAMGAFRHAVQDAVVQFQEDMNLYTLISVPVMLGSTIPAAPPGTQPGTLQPPMILLDFPPLACFLNNVLMAFNDLRLCCPVALAQDVTSCLEEALLKVTKLILVFQRAEEAAFSDRERELFTQFCCAFAEDMVPFLNRCLQVLFPPAQVALILGIQPTQVHKHGSLGCINVGEILEPLAFILPKKEVLPPAGELGSDLGSAGDVPAGGGSSPTQEPPHPPLSRLEDAATPVVQSTPEEAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0C7</accession>
    <name>W5M0C7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Actinin alpha 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0C7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001834"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001839.1">
        <property type="protein sequence ID" value="ENSLOCP00000001834.1"/>
        <property type="gene ID" value="ENSLOCG00000001588.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153968"/>
    <dbReference type="HOGENOM" id="CLU_005217_1_1_1"/>
    <dbReference type="InParanoid" id="W5M0C7"/>
    <dbReference type="OMA" id="YNHSYMV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001588">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055001">
        <property type="term" value="P:muscle cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000156">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="664"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="288"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="769" mass="88831" checksum="E04F2DB1BBB773C9" modified="2014-04-16" version="1">ETSAKEGLLLWCQRKTAPYRNVNVQNFHISWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVAEQTMRAMQQKLEDFRDYRRVHKPPRVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIANAWKGLEQVEKGYEEWLLTEIRRLERLDHLAEKFKQKCALHETWTRGKEELLSQKDYESASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAATVNSRCQGICDQWDNLGTLTQKRRDALERVEKLWETIDQLYLEFAKRAAPFNNWMDGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADKERMATMGIHSEILKIAQTYGIKLSGINPYTNLSSQDISNKWEAVKQLVPLRDQMLQEELARQQANERLRRQFAAQANVIGPWIQTKMEEIGHISVEMAGSLEEQMNQLKQYEQNIINYKSNIDKLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQLNEFRASFNHFDRVRKRRVGEGGRTGPPSTTSTGSPQKRNGMMDPDDFRACLISMGYDLGEVEFARIMTLVDPNNTGVVTFQAFIDFMTRETAETDTAEQVMASFKILASDKSYITVEELRRELPPEQAEYCISRMTKYVGTDAQPGALDYISFSSALYGESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0Q7</accession>
    <name>W5M0Q7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulator of telomere elongation helicase 1</fullName>
            <ecNumber evidence="13">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RTEL1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">A probable ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01021176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0Q7"/>
    <dbReference type="FunCoup" id="W5M0Q7">
        <property type="interactions" value="1065"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001964"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001969.1">
        <property type="protein sequence ID" value="ENSLOCP00000001964.1"/>
        <property type="gene ID" value="ENSLOCG00000001700.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1132">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182970"/>
    <dbReference type="HOGENOM" id="CLU_006515_4_0_1"/>
    <dbReference type="InParanoid" id="W5M0Q7"/>
    <dbReference type="OMA" id="EDPNTHS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001700">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904430">
        <property type="term" value="P:negative regulation of t-circle formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010569">
        <property type="term" value="P:regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090657">
        <property type="term" value="P:telomeric loop disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17970">
        <property type="entry name" value="DEAHc_FancJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13932">
        <property type="entry name" value="HN_RTEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000006">
        <property type="entry name" value="Regulator of telomere elongation helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000691">
        <property type="entry name" value="Regulator of telomere elongation helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03065">
        <property type="entry name" value="RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030845">
        <property type="entry name" value="RTEL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057498">
        <property type="entry name" value="Rtel1_ARCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049909">
        <property type="entry name" value="Rtel1_HHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23109">
        <property type="entry name" value="ARCH_RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23116">
        <property type="entry name" value="HHD_RTEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="9 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="7"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="785"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="793"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1117" mass="125388" checksum="900FC696A04C728C" modified="2014-04-16" version="1">MPKITLKGIAVDFPFTPYKCQEDYMNKVIECLQNRVNGVLESPTGTGKTLCLLCASLAWRDHFKDTISARKIAEKMGGVELFPDRPLSSWGSSATDGDTPAYYTDIPKIIYSSRTHSQLTQVIGELKNTAYRPKVCVLGSREQLCINPDVMRQESNHIKVHMCRAKMSTRSCLFYNNVEEKSNEKELVNTILDVEDLVKSGNKHRVCPYYLSRSLKQQADIIFTPYNYLLDPKSRRAHNIDLKGAVVIFDEAHNVEKMCEESTSFDLTPYDLATGIEAVNQVLVEQASQAGQNDVNEDFNVETLNSGLKMDIAIIAKIKRILLDLEAAIDSFEIPADGKGVTKPGSFIYELFRKVHLTFETKSSVLEALEQITGFLAGRPGVFINTAGLQKLSDIIQIVFSVDPPEGAPGFDMEQSIAKHYKVHIHRDTSSHKKKQSTDIWSSSSSKKQGNVLSYWCFSPGFSMQELLHQGVRCIILTSGTLSPLSSFTSEMQMYLFSLRSQRSGLQIVKMDITVLCMHLGYTFDQKLLQLSLHLERSSARPVRPCTNGDSIRGRHPPDPQRSFAQVSLFGSQASEGLDFSDSNGRGVIITGLPFPPRMDPRVILKMQFLDEMRRKASGGVKYLSGQEWYRQQASRAVNQAIGRVIRHREDYGAIFLCDHRFMSADARSQLPSWVRPYVRVYDNFGHVIRDVAQFFRVAQKIVPPPKGKASLSKCAAVCSSRDTSSSCAASASPLSARGLSVQKAKALDAHVPSLKKRRLDDPAAQDGLSRLCIEYESEMESGRRKPASLLDALDHNERKRGEEEEHLVGEEKASRLSTLSLQHDKRLEDELRGGKRRIKVVQDRDRSGSLSASEQTKADKAKAFMAGVRQALSQASYERFTKAMQQYKRADDLGAVLAELAELFAGDSNKHHLLRDFYQFVRPHHKRGFDEACQELTGKGCGFKPEHTLSKEEKEKLLLQTAANQPAIQNEGHQMNFNSPGLELLYAAVEIRACKDPHPSHQMVPKDKPQNLSAHKNQVLSTYLSDVKKAIGPAKYSQFHEAIQHYKKTDHYDSMVSTVVGLFTEKPEDFHLLRTFAMFVRPQHKQQFLQMFKELSGEEKQSSVNENLKAEQAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M119</accession>
    <name>W5M119_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cellular communication network factor 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M119"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002081.1">
        <property type="protein sequence ID" value="ENSLOCP00000002076.1"/>
        <property type="gene ID" value="ENSLOCG00000001790.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVMZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160207"/>
    <dbReference type="HOGENOM" id="CLU_063247_1_0_1"/>
    <dbReference type="InParanoid" id="W5M119"/>
    <dbReference type="OMA" id="RLVHHGC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001790">
        <property type="expression patterns" value="Expressed in bone element and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF22">
        <property type="entry name" value="CCN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867440" evidence="7">
        <location>
            <begin position="31"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="10">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="106"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="8">
        <location>
            <begin position="265"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="349" mass="38307" checksum="41E435CB51BC2076" modified="2014-04-16" version="1">EDQLASKNRSLQNTMFVWLLFLHVVTQVCCQLCGRPCYCPRRAPRCPAGVSVVLDGCQCCQVCARQEGETCNERFSCDRARGLQCDFSASYPGGPGVCVSEEELGCELDGVTYKEGQVFQPSCAMQCRCAGGGVTCVPLCSEDVRLPSPDCPNPQQLQLQGKCCKDWVCENTANSVLQDALAAHRPEVSNRLDPHSQTPNSGSNCIEQSTEWSACSQTCGMGTSTRVTNKNRACRLETQTRLCMVRPCQSLSRLAPTVSEGARRCEPSQKVLLPLLFEYQGCYSTQAFLPRFCGLCSDSRCCTPYQTRTVNVDFRCPQGRLVRHPMMVIDSCACHHSCPFSSSSTSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1J1</accession>
    <name>W5M1J1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1J1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002254.1">
        <property type="protein sequence ID" value="ENSLOCP00000002249.1"/>
        <property type="gene ID" value="ENSLOCG00000001944.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240385"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_1_1"/>
    <dbReference type="InParanoid" id="W5M1J1"/>
    <dbReference type="OMA" id="RNNDEHQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001944">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="202"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="397" mass="46262" checksum="CD73B8B14647B000" modified="2014-04-16" version="1">VLHPELLMSLESLQKEHSTLQKDHSDLQGRLSDLQRDYSNVTEHSQLTQQAKTGLQKKLETLQREHSNLQRDNSDLQRNYRNVTKSRLLTQKSNTDLQKKLQVLQREHSDLQRDHSDLQRRYSNVTGTFDQLQKALESLQSKHSSLQRELSDLQRNYRNVTDSYLLTQRSNTELQKTLESLQRQHSSLQRELSDLRRNYRNGWLKGRSYPHTRYKYYKTVDVTLDPNSAHPSLILSEDGKQVRKGDRRQTLPDFPKRFDPVSCVLGREGFSSGRHYWEVKVGEKTAWDIGVAKESINRKGKIILSPSNGYWTIWLTNANEFKAVANPPVHLPLSLKPQKVGIYLDYEEGQVSFYNVETRNHIYTFTDTFTEKLYPFYYPGLTDGGKNRNPLIISPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1T4</accession>
    <name>W5M1T4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rabphilin 3A-like (without C2 domains)</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1T4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002342"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002347.1">
        <property type="protein sequence ID" value="ENSLOCP00000002342.1"/>
        <property type="gene ID" value="ENSLOCG00000002023.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1013">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156758"/>
    <dbReference type="HOGENOM" id="CLU_117933_0_0_1"/>
    <dbReference type="InParanoid" id="W5M1T4"/>
    <dbReference type="OMA" id="TCPNDRE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002023">
        <property type="expression patterns" value="Expressed in ovary and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF4">
        <property type="entry name" value="RAB EFFECTOR NOC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RabBD" evidence="3">
        <location>
            <begin position="41"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="154" mass="17176" checksum="8842E5DBD0442C16" modified="2014-04-16" version="1">MTEAIFSNGTDQWVCPNDRQLALRAKLQTGWSVHTFRTDKQRKSQALSEGEMEVILGVIRRAELLEQAEQRRIGRLVERLENMRKSAMGNGLSQCLLCGELLGLLGTSSVFCQDCRKPVVQASGTRLTSSSTSHCVLNKVWRHGKTDCLQLSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1W1</accession>
    <name>W5M1W1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyroglutamyl-peptidase I</fullName>
            <ecNumber evidence="10">3.4.19.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PGPEP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01011226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1W1"/>
    <dbReference type="FunCoup" id="W5M1W1">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002369"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002374.1">
        <property type="protein sequence ID" value="ENSLOCP00000002369.1"/>
        <property type="gene ID" value="ENSLOCG00000002042.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4755">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015368"/>
    <dbReference type="InParanoid" id="W5M1W1"/>
    <dbReference type="OMA" id="KLAYNHK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002042">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000002">
        <property type="entry name" value="Pyroglutamyl-peptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="24409" checksum="74D6A43070F2CCF1" modified="2014-04-16" version="1">MPEGSTDDQAILNGCPSTPPHFNLHEGFGPFGEHTVNASWVAVQELKTLGLGNDVDLHVYEVPVEYQKVQSLVPSLWKQYHPQLVVHVGLSGMATTVTLEKCGRNHGYKGLDNCSFCPDSQCCVEGGPECIDSVIDMDSVCKRVTASGLGVAVSVSKDAGRYLCDYTYYTSLYLSHGRSAFIHVPPLDKPYSGEDLGRALRAIVLEMLDLLAQAEDKINCQHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3P2</accession>
    <name>W5M3P2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 29 member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3P2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003006.1">
        <property type="protein sequence ID" value="ENSLOCP00000003000.1"/>
        <property type="gene ID" value="ENSLOCG00000002549.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182898"/>
    <dbReference type="HOGENOM" id="CLU_021611_6_0_1"/>
    <dbReference type="InParanoid" id="W5M3P2"/>
    <dbReference type="OMA" id="YQCIPEA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002549">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015213">
        <property type="term" value="F:uridine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015853">
        <property type="term" value="P:adenine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015854">
        <property type="term" value="P:guanine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035344">
        <property type="term" value="P:hypoxanthine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035364">
        <property type="term" value="P:thymine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015862">
        <property type="term" value="P:uridine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034764">
        <property type="entry name" value="ENT1/ENT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00939">
        <property type="entry name" value="2a57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF8">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="408" mass="45083" checksum="86718BBA9129D5AF" modified="2014-04-16" version="1">AQYFQRRLRHEVNESAWANGTVPLQKEYHFNNWMTLLSQLPLLLFTFLNSFLYQWIAEKVRIAGSLVFILILFALTAALVKVPMEQDQFFSVTMATIWFINSFGAVLQGSLFGLVGILPQRYSAVFMSGQALAGTFAALAMLMAIASEADRETAALGYFITPCVGTLVTLVSYLALPHLEFARFYFSAGGPKTYELESNDELLRTENGPTENGKPNGQARLALENGPPSPAKKSSVIQVFKKIKVMALCVTLVFTITLSVFPAVTADVKTLYPGKWEAYFIPVCCFLTFNLMDWIGRSIPSVVQWPPKESRLFPALVAARLLFVPLLVLCNVQARARLPVWFAHDALFTAIMVLFSLSNGYCVCLSMSYAPQAERGPDAETAGALMTFFLALGLSLGAALSFPLRFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6H9</accession>
    <name>W5M6H9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Methyl-CpG binding domain protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6H9"/>
    <dbReference type="FunCoup" id="W5M6H9">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003987"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003995.1">
        <property type="protein sequence ID" value="ENSLOCP00000003987.1"/>
        <property type="gene ID" value="ENSLOCG00000003370.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183005"/>
    <dbReference type="HOGENOM" id="CLU_069710_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6H9"/>
    <dbReference type="OMA" id="SGKMPHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003370">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.890.10:FF:000003">
        <property type="entry name" value="methyl-CpG-binding domain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032343">
        <property type="entry name" value="MBD2/MBD3_p55-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025884">
        <property type="entry name" value="MeCpG-bd_2/3_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF5">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14048">
        <property type="entry name" value="MBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16564">
        <property type="entry name" value="MBDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="29806" checksum="46C1A30D5031A028" modified="2014-04-16" version="1">MERKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYYSPTGKKFRSKPQLSRYLGNTVDLGCFDFRTGKMMPSKLQKNKQRLRNDNLSQTKGKPDLNTALPIRQTASIFKQPVTKVVNHPSNKVKSDPQRATEQPRQLFWEKRLKGLRSCDVTEEVLKTMELPKGLQGVGPDTTDETLLSAIASALHTSSSPITGQTCNAVEKNPAVWLNTSQPLCKAFIVTDDDIRKQEERVQQVRKKLEEALMADGLARAAESTQDMSIENKANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6Z3</accession>
    <name>W5M6Z3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6Z3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004151"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004159.1">
        <property type="protein sequence ID" value="ENSLOCP00000004151.1"/>
        <property type="gene ID" value="ENSLOCG00000003502.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154838"/>
    <dbReference type="HOGENOM" id="CLU_004111_3_0_1"/>
    <dbReference type="InParanoid" id="W5M6Z3"/>
    <dbReference type="OMA" id="TVCFQLK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003502">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034687">
        <property type="term" value="C:integrin alphaL-beta2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF84">
        <property type="entry name" value="INTEGRIN ALPHA-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001426611" description="VWFA domain-containing protein" evidence="16">
        <location>
            <begin position="23"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1093"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="157"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="443"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="506"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="568"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1124" mass="122607" checksum="895FED42CA1795FE" modified="2014-04-16" version="1">MTPGRILFSLGILLSAGGVSEGFNIDTTSPIIYSGSSQDYFGYKTIQYGAGQEKWIIVSAPLKDNRTGGIYKCAYEPNSCQLILNEVTVMDSVKAVGLSLAARMGSNPQLTTCSPSLAHGCASNMYLNSICYQFDQHLQLTGNLTPSYQGCFKRDVDLVFLFDGSESLTPADFEKNKEFIVDIMRNLTNSSIQFAAVQFSTTFRTEFTFEQYQQRKNLAGLLKDAGHMKSLTNTHEALDFTVNHVLYNKTAGAKDNGTKVLIIITDGDPTDFDDYNIVDTLNNKGIIRYVIGVGNLDLRNLKKLSSEPKENNTFYIQNYGGLKNVLDNLQNKIYSIEGVSSTNKKKFEKEMSQTGFSALYNEDSLMLGAVGYNNWQGSVSEISPGLSETPVIPVNVSEDSYLGYALARGLRGPFSLLAAGAPRAEHRGTVLLFRKSESSTRWEEAFRLSGQQIGSYFGGELCSLDVDSDNQTDFLLVGAPLHYEDQREGMVFVYKVADEGFLSPVQNISGATSSPFSRFGSAMASLQDLNGDGLDDVAVGAPLENDHRGALYIYLGDREDGIKAQYSQHILAETLSPRLRYFGHSVSGLMDLSADGLTDITVGAMGDVIVLRSRPVLSVWGRLSFEPTQISLAHFDCLGVSSESPVLNLTVCFSVELDTKNTPGEEPDTTVCITSIDLLITYSVVICAHPESDCMTLSSGARCAVLSLYTSLQNCVKDTVSPVKIKLTFSQTEGPSSPALTPILNIDSRNVEYIEVPFEKNCGANDTCIADLHLDFNFSCAARPREGAAEPELTALLASPSSPSLLVVHHPVPGALLQASRRTLASCNVESPEKTLCSVSPPVYRGDTTAVFLNQFRISPEADWNETMEITVKAHSDNGKFSNETVLTKEIPVLFAIDVTVKGLDSNYFNFTVEDIASTDIKHEYEVKNEGQKGLPIDVAFTIPTQFSSNFWWNVQSISSDMLFHSSGWEFNLLCFLLLNGSPPAVLPAGSVASEQQTGAGCEKIENCPAMLEKGSSIHISFSGPSSFNVEDLKGKLSFSEERIQVDFSSSAQLSYDTRRYVQYSSSGASERLHPHFAHFLTKAELVIPPHTAFITGVGIGGGLLVLIILILILYKVGILSISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M714</accession>
    <name>W5M714_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ETS variant transcription factor 5b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M714"/>
    <dbReference type="FunCoup" id="W5M714">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004172"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004180.1">
        <property type="protein sequence ID" value="ENSLOCP00000004172.1"/>
        <property type="gene ID" value="ENSLOCG00000003519.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165279"/>
    <dbReference type="HOGENOM" id="CLU_030025_1_0_1"/>
    <dbReference type="InParanoid" id="W5M714"/>
    <dbReference type="OMA" id="MIPENQY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003519">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000121">
        <property type="entry name" value="ETS translocation variant 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006715">
        <property type="entry name" value="ETS_PEA3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF160">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04621">
        <property type="entry name" value="ETS_PEA3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="8">
        <location>
            <begin position="468"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="613" mass="68794" checksum="30004BB5CB56A54A" modified="2014-04-16" version="1">MSSDSDVHEPQGKPSASIEKSSFKFCQSESVHCLSGGQISLRFCVGFLKHCFSTLEGTIRHRKEEKERREALSRSLYASSPKPGTPAACRSRTMDGFYDQQVPFMVPGSSQVEGPRGRPVNERKRKFLDTELAHDSEELFQDLSQLQEIWIAEAQVPDDEQFVPDFQSDNLVFHGPPPNKIKRELQSPSKELSSCRQEQNLSMPYGEKCLYSYSAYDRKPPVGFKPLTPPSTPVSPCGSAHTPVSRSVALHEQASPPVPAQAQGARPLHGQTPPPGSTHSQRPHPLHSQTPPFAVPRPPVACPEGAYSTEHRFHRQLSEPCLPRGCPPYPSPAPSTAAPRDGRPPYHRQMSEPLVPAPPQGFKQELQDPRYAEQGAVAMGPPQPAFHPLAIKQEPRDYCFDSEVPNCHSSFGRGASFYPNSHDGFAFDREPHMYYDDTCVVPERLEGKVKQEPVVYREGPPYQRRGSLQLWQFLVTLLDDPANGHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPEALFTMAFPDNQRPSLKSDPDSLTGPEDDTLPLTHYDDGAPYLVDGAEQCVTGLPFHDGYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7N3</accession>
    <name>W5M7N3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">A-kinase anchoring protein 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7N3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004391"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004399.1">
        <property type="protein sequence ID" value="ENSLOCP00000004391.1"/>
        <property type="gene ID" value="ENSLOCG00000003685.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693584"/>
    <dbReference type="KEGG" id="loc:102693584"/>
    <dbReference type="CTD" id="11216"/>
    <dbReference type="eggNOG" id="KOG3590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015077"/>
    <dbReference type="HOGENOM" id="CLU_022662_0_0_1"/>
    <dbReference type="InParanoid" id="W5M7N3"/>
    <dbReference type="OMA" id="EFHCKYQ"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003685">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12804">
        <property type="entry name" value="AKAP10_AKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08735">
        <property type="entry name" value="RGS_AKAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08721">
        <property type="entry name" value="RGS_AKAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000018">
        <property type="entry name" value="A-kinase anchor protein 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000005">
        <property type="entry name" value="Putative A-kinase anchor protein 10 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037719">
        <property type="entry name" value="AKAP10_AKB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="104"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="342"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="624" mass="69361" checksum="39A143088F482DF8" modified="2014-04-16" version="1">MSFFRRKVKGKEHERPADVKTSKAPAPAPSPSLAVRNHHGILEAAGPSHVAINAISANMDSFARGRTAILKKQPSHMEATHFGDLGRSCVNYQAQETRSRLSKTVEQILRDGVAMPYFVQHMELQGADHLVKFWLEAESFRSVSWSRIRAHSLNSVKHSSLAEPASPLARPPRSGDPAEGDPQDSRTPSPAKGNPYSHARTSPNNSFSSDEKESDALQGGPKADTPTRQASSKAGTPFKLQPSHALKDLSEKLMKSIERDAVDIFTKYISPDAARSIPITEAMRTDIVAKICGEDGQVNPNCFVNAQSAVFNIMEQQHFSEFLRSHYFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEEAVNILQFWLAADNFQDQLAAKKGQYDGQEAQNDAMILYDKYFSLQATKPLGFDDSVRLEIESNICREGGPLPDCFTTPLRQAWTTMEKVYLPGFLSSNLYYKYLSDLINSVRGDDCITGNMLVPGHGLPQDSETGSSSSEGTHTQQGAKKAAIKILKNFDEAITVDIASLEPESLYQRPYAGRMTFGKVNELGQFIREAEPEPDVKKSKGSMFSQAMKKWVQGNSDEAQEEMAWKIAKMIVNDVMHQAQHESPCEKSTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8I9</accession>
    <name>W5M8I9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01008208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642562.1">
        <property type="nucleotide sequence ID" value="XM_006642499.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8I9"/>
    <dbReference type="FunCoup" id="W5M8I9">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004697"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004705.1">
        <property type="protein sequence ID" value="ENSLOCP00000004697.1"/>
        <property type="gene ID" value="ENSLOCG00000003930.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692925"/>
    <dbReference type="KEGG" id="loc:102692925"/>
    <dbReference type="CTD" id="100310831"/>
    <dbReference type="eggNOG" id="KOG3736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155456"/>
    <dbReference type="HOGENOM" id="CLU_013477_4_0_1"/>
    <dbReference type="InParanoid" id="W5M8I9"/>
    <dbReference type="OMA" id="WHRGNKS"/>
    <dbReference type="OrthoDB" id="9924649at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003930">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23475">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000012">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF37">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="19">
        <location>
            <begin position="472"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="609" mass="70014" checksum="3138D69BAC8E25D2" modified="2014-04-16" version="1">MACTRKTKTLVSTCVILSGMTNIVCLLYVGWVTNYIANVYIKVQEPVPEKKLEDDNKGETLRIIERLDRLENVVNQHIQEAPPKEDENGAVESFSDSSLFAHWGQDLSPENRRIALKKFQYYGYNAYLSDRLSLDRQIPDLRPEGCKNISYPTNLPQVSIVFIFVNEALSVILRSIQSAIQRTPTHLLKEIVLVDDNSSNEELKEKLQEYVGEMNTRYPGFVKMIRHTKQEGLIRSRVSGWRAASGPVVALFDAHVEFNVGWAEPILQRIKEDRTRIISPSFDNIKYDTFEIEEYPLSAQGFDWELWCRYLNPPKSWWNLGNTTAPIRSPALIGCFVVDRQYFEEIGLLDEGMEVYGGENVELGVRVWQCGGSVEVLPCSRIAHIERAHKPYTEDLTAHVRRNALRVAEVWMDEFKSHVYMAWNIPQEDSGIDIGDISERKALRKKLQCKTFRWYLVNIYSEMRMYTETIAYGVLKNSLKTDLCLDQGPDTDNVPILYICHGMTPQNVYYTSTQQFHVGVLSPTIDDDDNKCLVDVNSRPRLIECSYAKAKRMKLYWLFTQGGSIQNRKSKRCLELMETSDNEFGFQLVLQRCSGQRWTITNVLSGATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8T3</accession>
    <name>W5M8T3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized LOC107078986</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8T3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004792"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004800.1">
        <property type="protein sequence ID" value="ENSLOCP00000004792.1"/>
        <property type="gene ID" value="ENSLOCG00000004012.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078986"/>
    <dbReference type="KEGG" id="loc:107078986"/>
    <dbReference type="CTD" id="503594"/>
    <dbReference type="eggNOG" id="ENOG502S3ZF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000069547"/>
    <dbReference type="HOGENOM" id="CLU_372122_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8T3"/>
    <dbReference type="OrthoDB" id="8962741at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004012">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019979">
        <property type="term" value="F:interleukin-4 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002532">
        <property type="term" value="P:production of molecular mediator involved in inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015319">
        <property type="entry name" value="IL-4_rcpt-alpha_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF42">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT GAMMA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09238">
        <property type="entry name" value="IL4Ra_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865811" evidence="8">
        <location>
            <begin position="20"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="121"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="747" mass="83581" checksum="BEA1A88DF2268DF6" modified="2014-04-16" version="1">MNYMVLAILIQLTYSLVHAELDTESLKCHNDYETTMICEIDAEGPEDCNNSSALVFSGTNSEKNYTCTLVYKNADFSSLKTHCWCKTQMPRFVYNDNYTVYLLTGKISSHLKNIIPCNNIKPRAPHILNVKHGENGNFRVTWENNYSNESFLYDSLNFQLSYGIKEKGDEVATVLNVSDFFYEILKSSLNPGRDYVVKVRSYSSNYQTQFSDWSQELEWHNSMTVPDILKVGIPVLCVLLIITIVSSYRFYTRLKKSWWDKIPNPGNSRINHVYSANTKLVLSPLYCKPIINNLTLDMQTSGIIEKLWPPSLDGDATLEKFNSVISKTVEEDQKVVVTESPYHGCSSSYEADYPDTITTYSLTKERDTANSEIITQTQNALRALFCQHDIFHAHHPKAFIPTSVLHNSMDVCNAYSGSDNCALMKNRNPGCGELNVLPFTTDGQAHHFRSSVSSTGSSESGYKSIPYSWVSLKEYPVFKMESSKELVLSERPPMTAQPEVWCDPSYQGLCLNTCLNNNEKCPLSSMNTDSLKGPFLPTPVQTISEYQSYDGNGTLLTNGTTVFLSSRSLLCGAGNLLSSPGALQLADGYQSLNDTRTKRNSDVTNFSVATTEQTPTDIDWQLETKLICNVNPAYADLKISACSTSSCVEGYQPFQTMVEKISIDKPPVQHLKHVVSSKSLQDLQLSCFSGLNLTEIPKDGPTICVSDYHGLQNPHTRINPDKDSHPGSVIAIPEMTIMPIDSTYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8U7</accession>
    <name>W5M8U7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="17">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family. USP20/USP33 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01025369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8U7"/>
    <dbReference type="FunCoup" id="W5M8U7">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004806"/>
    <dbReference type="MEROPS" id="C19.025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004814.1">
        <property type="protein sequence ID" value="ENSLOCP00000004806.1"/>
        <property type="gene ID" value="ENSLOCG00000004021.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690505"/>
    <dbReference type="KEGG" id="loc:102690505"/>
    <dbReference type="CTD" id="10868"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158829"/>
    <dbReference type="HOGENOM" id="CLU_004896_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8U7"/>
    <dbReference type="OMA" id="IDQDDEC"/>
    <dbReference type="OrthoDB" id="73004at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004021">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021551">
        <property type="term" value="P:central nervous system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904888">
        <property type="term" value="P:cranial skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000001">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000002">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000065">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000359">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF13">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 17" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="20">
        <location>
            <begin position="6"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="19">
        <location>
            <begin position="148"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="21">
        <location>
            <begin position="682"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="21">
        <location>
            <begin position="784"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="253"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="907" mass="101544" checksum="213FE7E48207AB5D" modified="2014-04-16" version="1">MTENGELCPHLDSIGEVTKEELLQKSKGTCQSCGAGGPNLWACLQKDCPYVGCGESYADHSTLHAQAKTHNLTVNLTTFRIWCYVCEREVFLEQRPVTPVPSSHQCKHSEQEAVPQTGSHPLKAVPIAVADEGESESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLVECGEPEGEARREGDRSPSEDEFLSCDSGSSSDRGEGPEGRGAGEAELLITDECGGGGRGGISEKERLKERRFSGSPHRTGSQEMDEDADVDTAAEEGRGASRAGDEEVTASNNQCSQSLSRTPEPDNEAAMHCNSRPCSPIHGLQDSHPKLSSSPLRSGATYSLKKAQLLLGSKKKKQFRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQNLPVKAGTCSDTYSSQGWISFIMDYIRRFVVSCIPNWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQKVVALANMKEHSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGIPPNKYHYIDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCSICQVEIEALAKRRKVEIDTFIKLNKAFQAEEAPTVIYCISMQWFREWESFVKGKDNEPPGPIDNSKIGVMKGGHIQLKQGADYGQISEETWHYLFNIYGGGPEIAVRQTVAQSDTESLHGERKIEAETRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9W5</accession>
    <name>W5M9W5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-type tyrosine-protein phosphatase F</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01018282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="4" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
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    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="27"/>
        </location>
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            <begin position="28"/>
            <end position="1906"/>
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        <location>
            <begin position="1261"/>
            <end position="1284"/>
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            <end position="817"/>
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            <end position="915"/>
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            <end position="1096"/>
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        </source>
    </evidence>
    <sequence length="1906" mass="212852" checksum="17ED023DB614BD11" modified="2014-04-16" version="1">MVPHCCSATVLVLVAAGLGCLVLSSQADSLPSFIKSPEDQIGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSMGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDIAGSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMAGVVELTKEDEMPVGRNVLELTNIRESANYTCVAISSLGMIEATAQISVKALPKPPTSLIVTETTATSVTLTWDSGNSEPVTYYVIQYKAKSSESGFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSDIVDARTGEQAPSSPPLHVQARMLSASTMVVQWEPPEEPNGQIRGYRVYYTTSLQSPLNTWQKHNTDHSRLTTIPNLTPDTTYSIRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQESITKYELKYWEASSPNKEIHVTFNPTGSYAVEGLKPDTLYRFSLAARSEMGLGVYTQPIEARTAQSTPSAPPQDVQAVSLSSTSIKVSWVAPPAGSRHGAIAQYTVAYQAVTGEDAEKHEVTGLAPEASSYVLDGLEKWTEYRVWVRAHTDVGPGPESLPVHVRTNEDVPGAPPRKVEVEAVNSTAIRVTWKAPLSTKQHGQIRGYQIIYSRLENGEPRGQPNIMDVALPEAQWKVEETTDYEAIITGLQSETTYSVTVAAYTTKGDGARSKAKVITTTGAVPGKPSMMISTTVGNTALIQWQPPKEMVGELIGYQLQYKRVEEESYIVRDFKKTDDHYTVTGMHKGATYIFKLCAKNRAGNGEEFVKEITTQEDVPSGFPQNLRVVGLTTSTTELAWEPPLLSERNGKIVQYIVVYRDINSQQNHTNSTSDTYITISGLRPDTTYDIRVRAFTSKGAGPISPSIQSRTMPVEPVFAKSFQVKAVMKTSVLLTWEVPESYKSQVPFKILYNQQSVEVQGKLKRKLITKLQPDTDYSFVLMSRGNSAGGLQQVVSIRTAPDLLKSKPVIYRGQEEDGKLTISLNRVCISDRSSCLKLIITCFFITESYQWTDGVSQPYNKGLKSSKEGACPRVKESRKPYSAPLSSLQLVTAGPENTFPSLSRRNYLKFCNNKVKTNHFKSLEILKKNLPYFYTLFQKTFAASPYSDPITVEVVNRMPRQPEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRASPSPKDDHSGGVKDSLLAHSSDPVEMRRLNHQTPGMREHPPISVSDLADHIERLKANDGLRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTNTIIMMTRLEEKSRVKCDQYWPSRGTETYGMIQVTMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQVPPGETVTSMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINGSFIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9X1</accession>
    <name>W5M9X1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRK SH3-binding GNRP</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9X1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005180"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005188.1">
        <property type="protein sequence ID" value="ENSLOCP00000005180.1"/>
        <property type="gene ID" value="ENSLOCG00000004322.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156235"/>
    <dbReference type="InParanoid" id="W5M9X1"/>
    <dbReference type="OMA" id="HYRLRCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004322">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000009">
        <property type="entry name" value="rap guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF224">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="831"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="987"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="217"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="488"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="676"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="441"/>
            <end position="455"/>
        </location>
    </feature>
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        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
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        <location>
            <begin position="676"/>
            <end position="687"/>
        </location>
    </feature>
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        <location>
            <begin position="721"/>
            <end position="736"/>
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    </feature>
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        <location>
            <begin position="788"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00083313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1224" mass="135938" checksum="AD11817B9C62BACB" modified="2014-04-16" version="1">MAGEVKSSDSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKQVQPEPPVKVAEKPVNKNLSRLEEYEKDVVSALRYFKTIVDKMVVDKKVLEMLPGSASKVLEAILPLVQVDSRVQQSSAVSSCLNRVYQSLANLISWSDQVMLEGVNLEDKEAIVTVTGVIKAVLDGVKELVKLTIEKQEHPSPTSPVKPALSACKSESTAELPLSEREKEILSKTTPITQSTEILTDVTDEDVAPPKPPLPGLKVPEHSPPALPPKKRQSAPSPTRVAVVAPMSRTTSGSSLPVGIGKGDYDLDYLQRRLSGGSQSYGGESPRLSPCNSIGKLSKSDEQLSSLEQDSGQCSRNTSCETLDNTESYDPDYDFLHQDLSNADQIPQALVGGILSPLPECHNESSSPCPIMHFTSPFNTQPPEYWGSAGPQASPQPASAGGPPALPEKKRRSAASQASPHSDSSGSRVSYERHPSQYDNISEEDLQPVPPFPVFTAVSPHPQGNSGAFPAEFGASEMSDIPESPPPLPEKKSRHILKYMQFVEDYSEPQPSVFYQTPQSESIYEQRNKRFQEVYGFNDSFSSTDSVHEPQPPPALPPKQRQLQASYTASSSSSSSLSCHVQHSNPTTVAVEDASSALSLSMSVSNSFLSRHSSLTAPPYKTVFRSYSQDFAPHNQASIPPFLSSVSSHFPTVHQSQSSDLEAPATAGQSTDPGGMAHDSLESSGGSLAVCLPSESSLTDSLQPSVSESANEEGGEGEYVNLYSSSQANGELPHSKGEPTAVTDEVLQDPGPQKSSANSKEIADVERRQKSTDSGQSDEEVDELSLIDHKEIMARITLKQENDDGPDVRAGSGDILLVHATETDRKDLVLYCEAFLTTYRTFITPEDLIKKLHYRLRCTYTRFCHSPDTFKKRVSKNTFFVLVRVVDELCLVELTDDILKQLMELVFRLVCNGELSLARVLRKNILDKVEQKKLLRYTNSLKPLAARGVAARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIIQQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDFIDGKVNFSKRWQQFNILDSMRRFQQVHYDLKRNDDIVSFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAJ5</accession>
    <name>W5MAJ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CD200 receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD200R family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAJ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005404"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005411.1">
        <property type="protein sequence ID" value="ENSLOCP00000005404.1"/>
        <property type="gene ID" value="ENSLOCG00000004519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079420"/>
    <dbReference type="KEGG" id="loc:107079420"/>
    <dbReference type="eggNOG" id="ENOG502S9IV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204624"/>
    <dbReference type="HOGENOM" id="CLU_1677271_0_0_1"/>
    <dbReference type="InParanoid" id="W5MAJ5"/>
    <dbReference type="OrthoDB" id="8915654at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004519">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150077">
        <property type="term" value="P:regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040012">
        <property type="entry name" value="CD200R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462:SF2">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN CD200 RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN OX2 RECEPTOR PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="17238" checksum="B6B8B56C8175EBA1" modified="2014-04-16" version="1">MATCTAANSKPAANISWIGVSNWTEKTTKNPDHTYTVESSASIPDNTSLQNVSCVVEHPVWKEGVHKTVGLPLENFPSFPLTWTIISVSLSGFVVLILVASFFIRKHLSKIRNCCKSSIPAPPPANRKQDVEELEPYASYVERVNSIYNSSAELCNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBD3</accession>
    <name>W5MBD3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:ch211-210c8.6</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBD3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005692"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005700.1">
        <property type="protein sequence ID" value="ENSLOCP00000005692.1"/>
        <property type="gene ID" value="ENSLOCG00000004747.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008169"/>
    <dbReference type="HOGENOM" id="CLU_043418_1_0_1"/>
    <dbReference type="InParanoid" id="W5MBD3"/>
    <dbReference type="OMA" id="FQMLWVW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004747">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF17">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="256" mass="29743" checksum="E7B665FAF2B20E21" modified="2014-04-16" version="1">VMGNAVVKTAAIDLAIQWSCWAVSAVFKSEKIYDLAGTGSFILLTHLSRRWGGHSYTRQTVHGWLVTAWGLRLGTFLFLRMMAAGQDRRFNNIRDRPGVFWFYWTMQGVWVFTTLLPTVILNTERRNPPLGFRDYLGWALWGVGFLMEAIADQQKWNFRRDPDNAGKFIRHGLWAYSRHPNYVGEIMQWAGLFISASSVMQGTQYLTVTSPLILWFLIRYLSGVPPLEEYALKKWGADPQYQKYVKDTPVFWPFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC70</accession>
    <name>W5MC70_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01004392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC70"/>
    <dbReference type="FunCoup" id="W5MC70">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005979"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005987.1">
        <property type="protein sequence ID" value="ENSLOCP00000005979.1"/>
        <property type="gene ID" value="ENSLOCG00000004962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1559">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043388"/>
    <dbReference type="HOGENOM" id="CLU_058704_1_1_1"/>
    <dbReference type="InParanoid" id="W5MC70"/>
    <dbReference type="OMA" id="EPVSSHF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004962">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000024">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF20">
        <property type="entry name" value="GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866019" description="folate gamma-glutamyl hydrolase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 7">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="320" mass="36377" checksum="87DDD5B3B296FB24" modified="2014-04-16" version="1">GYCNLYFMEMNFLSHAFLLVVFLGCASSAVFKTAKINDRPIIGVLAQEIKSSENPEISYIAASYVKFLESAGARVVPVRINQSQEDYEKLFNSINGFLLPGGGVNLISSGYAKAASVFYQLAIKANSEGDYFPIWGTCLGFEELTYLTSGKILLVRTNTTSVALPLIFTKGARESRLFKNFPTELLEALATESITENSHIWSLSVEKYNSDEKLRKFYNILTTNKDEKLEFVSTMEAYDVPIYGTQWHPEKNAFEWTRPYIPHSPNAVKATFYMADFFVNEARKNFHHFPSEEEESKALIYNYIPVYKGNHSAFEQIYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCC1</accession>
    <name>W5MCC1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione hydrolase</fullName>
            <ecNumber evidence="6">2.3.2.2</ecNumber>
            <ecNumber evidence="6">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="6"/>
    <dbReference type="EC" id="3.4.19.13" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01005121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006030"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006038.1">
        <property type="protein sequence ID" value="ENSLOCP00000006030.1"/>
        <property type="gene ID" value="ENSLOCG00000005005.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154601"/>
    <dbReference type="HOGENOM" id="CLU_014813_4_1_1"/>
    <dbReference type="InParanoid" id="W5MCC1"/>
    <dbReference type="OMA" id="APACTTH"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005005">
        <property type="expression patterns" value="Expressed in mesonephros and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050727">
        <property type="term" value="P:regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000002">
        <property type="entry name" value="glutathione hydrolase 1 proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055262">
        <property type="entry name" value="GGT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF56">
        <property type="entry name" value="GLUTATHIONE HYDROLASE 1 PROENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-1199">Hemostasis impairing toxin</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1202">Platelet aggregation activating toxin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0800">Toxin</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="407"/>
            <end position="409"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="459"/>
            <end position="460"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00084097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="577" mass="63203" checksum="7328C354DB2CA0F3" modified="2014-04-16" version="1">YVCRKMKRKSVVVGVVVLLLAVLCIFLGIFYGLDSRAPPKEGYYTRAAVAADAGKCSEIGRDILKRKGSAVDASIAALLCVGLMNAHSMGIGGGLFLTIYDASTGQVEVIDARETAPRNATENMFGNNTQLSQKGGLSIAIPSEIRGYELAHMRHGRLKWKELFEPSIALARSGFPVGRALAAAINENRQTIEGDPALCEVFCNSEKSILKENDTIKFPRLADTYQKIADEGPDVFYNGSLAQDIVKDIQSAGGIITLEDLRNYKPELNLEPLKIKVGEYTMYVPNAPSSGPVLGLILNILEGYNFTSASVSTSETKTLTYHRILEAFRFAYAKRSRLGDPRYLNISDLIQNMTSDYTATAIRKKITDNTTHPVPYYEPEYYNPDDHGTAHLSVIAEDGSAVSATSTINQYFGSKVRSLSTGIIFNNEMDDFSSPFIINGFGVPPSPNNFIRPGKRPLSSMCPAILFDQNNKVKMVVGASGGTKITTATALVILNSLFFNYDVKKAVTEPRAHNQLWPNVTEVEKDFEKSIQAGLEQKNHEVELQKSRGAVVQAIVRQGEKLYAESDYRKGGSPAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCI9</accession>
    <name>W5MCI9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box transcription factor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222868.1">
        <property type="nucleotide sequence ID" value="XM_015367382.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222869.1">
        <property type="nucleotide sequence ID" value="XM_015367383.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCI9"/>
    <dbReference type="FunCoup" id="W5MCI9">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006098"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006106.1">
        <property type="protein sequence ID" value="ENSLOCP00000006098.1"/>
        <property type="gene ID" value="ENSLOCG00000005064.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687110"/>
    <dbReference type="CTD" id="9496"/>
    <dbReference type="eggNOG" id="KOG3585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158882"/>
    <dbReference type="HOGENOM" id="CLU_037025_1_0_1"/>
    <dbReference type="InParanoid" id="W5MCI9"/>
    <dbReference type="OMA" id="VCERKAP"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005064">
        <property type="expression patterns" value="Expressed in muscle tissue and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035119">
        <property type="term" value="P:embryonic pelvic fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20189">
        <property type="entry name" value="T-box_TBX4_5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000005">
        <property type="entry name" value="T-box transcription factor TBX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF29">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="9">
        <location>
            <begin position="49"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="445"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="539" mass="58968" checksum="8C2CAEF33BB80228" modified="2014-04-16" version="1">MLQEKVPAVTDEGVTELAPESAHLSLAPAASTPHSNEPDQNIENIKVFLHERELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFHETAFISVTSYQNHKITQLKIENNPFAKGFRGSEEGDMRVSRLQSKDYPVISKSIVRQRLVSSHGHFAGKLGAGVLPGHPQAIPHYQYESGTPLPGSDPPDPLVTPLPSTREPGVLYHCFKHRDSARPLELGCKRPYLDPAPPPVPEEHYFRPAPSYEPPLLSHSYCGEALGSRDACMYGGAEGEAAAAGGGGGTEDLPPPPPSLNCNMWASMPPYPGYSVQTVEAVSYQPFTAHFASTATAMMPRHPVPMATQLPPPASPDLGAYSSPPAHRGPHSSSSSSSSPPAPSSRDRPPASLYQRKPGSPPLPARDFPAFTPPPIRDPSYQYHLGLSGAGAHWTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCJ8</accession>
    <name>W5MCJ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Meiosis specific with OB-fold</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the MEIOB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCJ8"/>
    <dbReference type="FunCoup" id="W5MCJ8">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006107"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006115.1">
        <property type="protein sequence ID" value="ENSLOCP00000006107.1"/>
        <property type="gene ID" value="ENSLOCG00000005071.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001723"/>
    <dbReference type="HOGENOM" id="CLU_042457_2_0_1"/>
    <dbReference type="InParanoid" id="W5MCJ8"/>
    <dbReference type="OMA" id="IYLKFVV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005071">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000239">
        <property type="entry name" value="Meiosis specific with OB domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000171">
        <property type="entry name" value="meiosis-specific with OB domain-containing protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000527">
        <property type="entry name" value="Methionine sulfoxide reductase B1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052469">
        <property type="entry name" value="MEIOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056880">
        <property type="entry name" value="OB_MEIOB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21166">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24 OB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21166:SF2">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24 OB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24903">
        <property type="entry name" value="OB_MEIOB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MEIOB-like N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="471" mass="52311" checksum="13F765E4D0388F3A" modified="2014-04-16" version="1">KMSFNAPLQNCISISDLHPSTTHPNVEGVVIGKTDIKGFPDRKNIGSERHTFRFTIKDSPDHFINVFSWGRENFIRALSDSFRIGDCVVIENPLIVTKDVEKEEQFNPSTPSFYKLLVSENHSTVRVCSDIERETRLLSLFHLPVKDSQDFYPLGDIIANGQSLDGKVINILAAVRSVGGPKYFTTADRRKGQRCEVKLFDETVPSFPLICWDNEAIHLAQTWIPIETVLFIADARITFDRFRHSMAATVTSKTIITVNPDTKEANLLFNYAGEFVKTGGLDDPDDLAADGVNLDSIVDVFTVHQLKLRAHENLDGPDSIYGITYAFVTTMNLDSSCSKLIRNRCARCMFLINEETQSCSSITCSESGNPTEAIASFDLLVDLTDHTGTLKSCNLSGTAAEKTLGCTAEEFICLTEGQKTALKWKFLLERCKVYHKVLPAPNSRSGLRVNVLSCTLADPVETGQSFSRSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCX8</accession>
    <name>W5MCX8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAS-associated death domain protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FAS-associating death domain-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Fas-associated death domain protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Can self-associate. Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits. Interacts (via death domain) with FAS (via death domain). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and PIDD1. Interacts with RIPK1 and TRADD. Interacts with stimulated TNFRSF10B. Interacts with DDX24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01035528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCX8"/>
    <dbReference type="SMR" id="W5MCX8"/>
    <dbReference type="FunCoup" id="W5MCX8">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006245.1">
        <property type="protein sequence ID" value="ENSLOCP00000006237.1"/>
        <property type="gene ID" value="ENSLOCG00000005182.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690370"/>
    <dbReference type="KEGG" id="loc:102690370"/>
    <dbReference type="CTD" id="8772"/>
    <dbReference type="eggNOG" id="ENOG502S2RV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002105"/>
    <dbReference type="HOGENOM" id="CLU_087961_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCX8"/>
    <dbReference type="OMA" id="CKMNLVA"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005182">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031265">
        <property type="term" value="C:CD95 death-inducing signaling complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089720">
        <property type="term" value="F:caspase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005123">
        <property type="term" value="F:death receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08306">
        <property type="entry name" value="Death_FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08336">
        <property type="entry name" value="DED_FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000059">
        <property type="entry name" value="Fas-associated via death domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000062">
        <property type="entry name" value="Fas-associated via death domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077:SF10">
        <property type="entry name" value="FAS-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="13">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="12">
        <location>
            <begin position="103"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="194" mass="22771" checksum="80E79029EBBD4C49" modified="2014-04-16" version="1">MSGFLDVLYQISQRLSKDNLNSLKFLCVEDIKKKKLEQIHQGTELFQCLIEMNKIGPDSTEFLHYLLGHIDRQDLIELLQKFERDGPAGLNDNQPDVKEREKLDAAFGVIVENMGRDWRMFARKLGITDAKLDHIREKHPFNMEEQVREVLRVWQKSKGKEAKVMDLIEKLRECKMNLTADFVEKELDSMKNFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDN2</accession>
    <name>W5MDN2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Syntaxin-17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Autolysosome membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPII-coated vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Autophagosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Smooth endoplasmic reticulum membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDN2"/>
    <dbReference type="FunCoup" id="W5MDN2">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006491"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006499.1">
        <property type="protein sequence ID" value="ENSLOCP00000006491.1"/>
        <property type="gene ID" value="ENSLOCG00000005385.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214440"/>
    <dbReference type="HOGENOM" id="CLU_058244_1_0_1"/>
    <dbReference type="InParanoid" id="W5MDN2"/>
    <dbReference type="OMA" id="YPVMGAL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005385">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120281">
        <property type="term" value="C:autolysosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030868">
        <property type="term" value="C:smooth endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000046">
        <property type="entry name" value="syntaxin-17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059001">
        <property type="entry name" value="STX17_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF139">
        <property type="entry name" value="SYNTAXIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26585">
        <property type="entry name" value="STX17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="16 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="19" id="KW-0458">Lysosome</keyword>
    <keyword evidence="18 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="28">
        <location>
            <begin position="173"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00055921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00069804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="329" mass="35917" checksum="CCD53A77A939F913" modified="2014-04-16" version="1">RLGWEGSRRGSMADEAGSLPLRRLEVPIHKFIKVAIPTDLERLRKHQINIEKYQRCQQWDRLHQEHINASRTVQQLRANIREMEKLCARVRSEDTRALDRLVRPTREQASVAAGDFLRLHSETAAPTGVQPTGPESPPAPSLSRSLTALGSFSGGEESSIPLQTQTQLPLPEIPRDQNAAESWETLEEDLLELNGLVNEFAELVHSQQEKIDSIEDHVNTAAANVQEGSRSLGQAARYKMAMLPVAGAVIGGVVGGPLGLLAGFKVAGVAAAVSGGLLGYAGGNLLQKSRRAKVDTQLQQLSTSCPELCKQEDKKKQCYSNTFPVPVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEX2</accession>
    <name>W5MEX2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Rhomboid-related protein 2</fullName>
            <ecNumber evidence="4 13">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4 13"/>
    <dbReference type="EMBL" id="AHAT01008029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEX2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006931"/>
    <dbReference type="MEROPS" id="S54.002"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006939.1">
        <property type="protein sequence ID" value="ENSLOCP00000006931.1"/>
        <property type="gene ID" value="ENSLOCG00000005736.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688448"/>
    <dbReference type="KEGG" id="loc:102688448"/>
    <dbReference type="CTD" id="54933"/>
    <dbReference type="eggNOG" id="KOG2289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159442"/>
    <dbReference type="HOGENOM" id="CLU_048023_0_0_1"/>
    <dbReference type="InParanoid" id="W5MEX2"/>
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    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005736">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000007">
        <property type="entry name" value="Rhomboid like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
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    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF6">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="17">
        <location>
            <begin position="105"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006931.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="32108" checksum="6BF306D1C337E31A" modified="2014-04-16" version="1">MAAEEDNPFNGEGEAEETVEETSEGCGWCERFQRSVAKWMLPEHARDIYLERANCCPPPIFIILISIGELAVFIYYAVWKPQKQWITLDKGILNSPLTYRPDKREEAWRFISYMFVHAGVEHILGNLTLQLLLGIPLELVHKGIEVGTVYLSGVLAGSLASSIFDPLHALVGASGGVYALIGGYFMNALVNFKEMIPLLGAFRIVAILLIVGTDVGFALYRRFISHEVGHAVSFVAHIAGGMAGMTVGFVFFSNYNQKLLKDPKFWVCIVGYFAFILFAVLFNIFLSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF31</accession>
    <name>W5MF31_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prothrombin</fullName>
            <ecNumber evidence="2">3.4.21.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Coagulation factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.</text>
            <dbReference type="EC" id="3.4.21.5"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.5" evidence="2"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF31"/>
    <dbReference type="FunCoup" id="W5MF31">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006990"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006998.1">
        <property type="protein sequence ID" value="ENSLOCP00000006990.1"/>
        <property type="gene ID" value="ENSLOCG00000005789.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTSX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164059"/>
    <dbReference type="InParanoid" id="W5MF31"/>
    <dbReference type="OMA" id="HERSGYE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005789">
        <property type="expression patterns" value="Expressed in liver and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030194">
        <property type="term" value="P:positive regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vitamin K-dependent protein S"/>
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    </dbReference>
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        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.140.10">
        <property type="entry name" value="Thrombin light chain domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003966">
        <property type="entry name" value="Prothrombin/thrombin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051659">
        <property type="entry name" value="Serine_Protease_S1-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018992">
        <property type="entry name" value="Thrombin_light_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037111">
        <property type="entry name" value="Thrombin_light_chain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24254">
        <property type="entry name" value="PROTHROMBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24254:SF10">
        <property type="entry name" value="PROTHROMBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09396">
        <property type="entry name" value="Thrombin_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01505">
        <property type="entry name" value="PROTHROMBIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0011">Acute phase</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0420">Kringle</keyword>
    <keyword evidence="7 20" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 20" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866057" description="Prothrombin" evidence="21">
        <location>
            <begin position="20"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="24">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="22">
        <location>
            <begin position="119"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="22">
        <location>
            <begin position="213"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="23">
        <location>
            <begin position="360"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="70906" checksum="7A4CE0BDA10A06D5" modified="2014-04-16" version="1">LHSLLVFFSNLIFLCSINSVNRFCGILVFLNRQKASMVLTRGRRANTVFEELKPGNMERECLEEICDHEEAREIFEDNTRTADFWKKYLACEGDQGKRNSKKIESLRLCLDGKCVMGRSGDKYRGNLSVTVSGRECQYWSSNFPHKKEFTLSMYPDKDLVQNYCRNPNNDTRGPWCYTRDPLVKKEECSISVCGEPPRPTPVPGKKVVYDKRECLKDAGIDYNGNISTTVTHHPCLSWSSPTARDLRHGKDFLPEVQLEKNYCRNPDGDLEGPWCFVDKPRIAFEYCDIPLCDEPLHEFQDDQEKISGRTVSAGSYKTFFNPNSFGQGEADCGIRPMFERVNKQDATEKELTESYIASRIVKGVEAEQGSAPWQVMLYRKNPQELLCGASLLSDQWILTAAHCILYPPWDKNFTANDIIVRLGKHSRAKFEKGQEKIVALDKIIVHPRYDWKVNLNRDIALLHMRNPVSFSDRIAPICLPTKEVAKTLMFAGFKGRVTGWGNLFETWSSSPSSLPSTLQQIHLPIVDQDTCRASTTIRITDNMFCAGFKPEDSKRGDACEGDSGGPFVMKNPDDQRWYQIGIVSWGEGCDRDGKYGFYTHLFRMRRWLLKVIENPVDGSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFM8</accession>
    <name>W5MFM8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex protein 3 homolog</fullName>
            <shortName evidence="5">NOC3 protein homolog</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFM8"/>
    <dbReference type="FunCoup" id="W5MFM8">
        <property type="interactions" value="1794"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007187"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007195.1">
        <property type="protein sequence ID" value="ENSLOCP00000007187.1"/>
        <property type="gene ID" value="ENSLOCG00000005949.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008540"/>
    <dbReference type="InParanoid" id="W5MFM8"/>
    <dbReference type="OMA" id="HYCPQVR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005949">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="554"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="449"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="805" mass="92550" checksum="8A7DA4FCCF8F3809" modified="2014-04-16" version="1">MGPRRSKKRTPSFRKLLKTSNLKIENKLKNRQFKQQSSAKKYRKEQKKLRQSLKDASSRIPAPLERYKKRPVVRPEEEEDEEFEETLPVDMMEEDDLEQMKALARKASFLTRDLSSCAPVHGKKRKLDNVVENYEKMPRKMQKREEKEVIHLLPIKDKTGVIPQSMEKPVLEKVEEEAEEEDTNAEEVSVPEPLPVLSRQEMLSQRRQRLEERKAHIAELGSAILSEPVNNIKKVKELRSMLMEVDPFVAVTVRKLVMVSLMEVFKDIVPSYRIRPLTEAEKATKVKKETQQLREFEQGLVSQYKFYLENLEQTVKDWKQMKKKKSDAVSLKAYRGLAEVALKCLCELLTAFPHFNFHNNIIVMIVPLMNDPSRKVSDMCCEAVKKMLKQDKVGQASLGMVKVISGLVKSRNYDVKPEVLKTLLCLRIKEMEVMKDAEDVAPKKKFMTFKEKRKNLSRMQRKWKKAEEKLERELLEAEASESKEKKMKLHTETLNIVFLIYFRILKKAQKSVLLPTVLEGLAKFAHLINVEFFDDLLMVLHNLIESGVLSYRESLHCVQTAFHVLSGQGDVLNIDPLKFYTHLYKTVLKLHAGVSNDDMVIVLQCLDIMLTKRRKQVTLQRALAFIKRLTTLSLHVLPNSTIGILASDRVLMHTFPKTDILLDNESQGSGIYLPELDEPEYCNPQNTALWELQTLQRHYHPIVRKFAAHLAAGAPVEGSRALDLELSRRSAVDLFENYSVKGMTFNPPVAEPSSKKKDRFTIGEAVLNAELNKQIDAVLGAPSASTVMDFAAHVRRPDGPREMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG61</accession>
    <name>W5MG61_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="27">Matrix metallopeptidase 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG61"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007370"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007378.1">
        <property type="protein sequence ID" value="ENSLOCP00000007370.1"/>
        <property type="gene ID" value="ENSLOCG00000006100.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684428"/>
    <dbReference type="KEGG" id="loc:102684428"/>
    <dbReference type="CTD" id="561041"/>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156939"/>
    <dbReference type="HOGENOM" id="CLU_015489_8_1_1"/>
    <dbReference type="InParanoid" id="W5MG61"/>
    <dbReference type="OMA" id="GCQEHVD"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006100">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
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    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF25">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
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    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 25" id="KW-0732">Signal</keyword>
    <keyword evidence="5 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016748450" evidence="25">
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            <begin position="34"/>
            <end position="649"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="606"/>
            <end position="627"/>
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    <feature type="domain" description="Peptidase metallopeptidase" evidence="26">
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            <begin position="122"/>
            <end position="292"/>
        </location>
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    <feature type="repeat" description="Hemopexin" evidence="22">
        <location>
            <begin position="349"/>
            <end position="397"/>
        </location>
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    <feature type="repeat" description="Hemopexin" evidence="22">
        <location>
            <begin position="398"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="22">
        <location>
            <begin position="445"/>
            <end position="493"/>
        </location>
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    <feature type="repeat" description="Hemopexin" evidence="22">
        <location>
            <begin position="494"/>
            <end position="541"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="286"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="552"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="21">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="317"/>
            <end position="328"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="331"/>
            <end position="346"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
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            <begin position="570"/>
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            <position position="247"/>
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        <location>
            <position position="100"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="183"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="193"/>
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        <ligand>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="195"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
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            <position position="200"/>
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        </ligand>
    </feature>
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            <position position="201"/>
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            <name>Zn(2+)</name>
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        </ligand>
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            <position position="219"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="221"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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    <feature type="binding site" evidence="19">
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            <position position="223"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="226"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="20">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR621190-5"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="649" mass="74237" checksum="E928940BB0393692" modified="2014-04-16" version="1">MMGSLWKCRTWLPWKLNVVPVILVFVCTVGARAAEEDATFNAESWLRMYGYLPQASREMSTMRSAQIFSNAVADMQRFYGLTVSGVLDQETQNAMKRPRCGVPDKFGGLIKTNVRRKRYALTGHKWSKSHLTYSIQNYTPKIGEFESYQAIRKAFQVWESVTPLRFEEIPYHEVKYGRRKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGMGGDTHFDSDEPWTIGNANLNGNDLFLVAVHELGHALGLEHSNNPLAIMAPFYQWMDTENFQLPEDDLRGVQQIYGPPDASPTPTKPLPPVEPRQPAHPDPKPPNPPKTPPGGKPHNPDPGKPRTTDRPDHYGPNICDGNFDTVTVLRGEMFVFKGRWFWRVRRNRVLDNYPMPIGHFWRGLPGDIDAAYERHDGKFVFFKGSKFWLFREANLEPGYPQELTEYGQGIPYDKIETAIWWEPTGYTYFFKGDRYWRFNEQSRAVDKDYPKPISVWGGIPPSPRGSFLSDDGAYTFFYKGSKYWKFDNQRMKIEPGYPKSILRDFMGCQVDLDLDKDTDTDVGRKWPDVNQPPFDPDAGRDRTNDVDYKDEGEGNGDVDVVVKIDEYTRTMNIIMVTVPLILVLCILGLIFAIIKMQRKGAPKLLVHCKRSLQDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIW7</accession>
    <name>W5MIW7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclic AMP-dependent transcription factor ATF-7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation. In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes. Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation. In response to TNF-alpha, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer; binds DNA as homodimer. Heterodimer; heterodimerizes with other members of ATF family and with JUN family members.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5MIW7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008326"/>
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        <property type="gene ID" value="ENSLOCG00000006882.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006882">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:cAMP response element binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12192">
        <property type="entry name" value="GCN4_cent"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic AMP-dependent transcription factor ATF-2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bZIP"/>
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        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
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        <property type="entry name" value="TF_CRE-BP1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
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    </dbReference>
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        <property type="entry name" value="ATF2_CRE-BP1"/>
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    </dbReference>
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        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
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    </dbReference>
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        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="343"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="246"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="423"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
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        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
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        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="253"/>
            <end position="264"/>
        </location>
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        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00023159"/>
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        </source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="PROSITE" id="PS50157"/>
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            <dbReference type="PROSITE" id="PS50217"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="497" mass="53049" checksum="A9EDFA66B141ED67" modified="2014-04-16" version="1">RMGDDRPFVCNAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARTDSVIIAASPDQTPTPTRFLKNCEEVGLFNELASSFEQEFRKVVEDEEDKRGKPLLASSQPGALDMTLTPPSEVKVKEEEPVEVDSSPPSSPDSMSSSMSDYKEPQENPPKPAVSSAPTPTIVRPGSLPLHLGYEALQPTLPSPTSVITQAPPSNRQLGSPTGPYPVLMHLPNGQAVPVLPGPPVQMPSVISLARPVSMVPNIPGIPGPPLGSSSGSSSPSGHIQHSEAKMRLKAALSQQAPMAPNGGGMAMGSSPMVPQRQEQSQLLVQHPDAPSPAQPQVSPAQPTGGRRRRAADDDPDERRQRFLERNRAAASRCRQKRKLWVNSLEKKAEELTSMNVSLSNEVSLLRNEVAHLKQLLLAHKDCPVTTLQKKNAYLVGEESPKDPSEPTGSPAPVIQHSSLSSSPAAQPNGLSARAAAEAVAMSVLAGMGSQRAEPNPAHVIMAPQSQPAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIZ2</accession>
    <name>W5MIZ2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synaptotagmin Ib</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5MIZ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008351"/>
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        <property type="gene ID" value="ENSLOCG00000006906.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Synaptotagmin"/>
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        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
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        <property type="entry name" value="SYNAPTOTAGMN"/>
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        <property type="entry name" value="C2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="48"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="172" mass="19677" checksum="0358AE8EF0FBE443" modified="2014-04-16" version="1">MMAVYDFDRFSKHDAIGDVKVPMNSVDLGHLIEEWRELESAEKEEQEKLGDICFSLRYVPTAGKLTVIILEAKNLKKMDVGGLSDPYVKIHLMQGGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKLGKNDAIGKVFVGCNSSGTRCSPRRKWRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJB5</accession>
    <name>W5MJB5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metaxin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial outer membrane import complex protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport of proteins into the mitochondrion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MTX1/metaxin-1. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
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    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
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    <sequence length="276" mass="31252" checksum="47E9AE822637A3D7" modified="2014-04-16" version="1">MSLAAEAFVSQIAAAEPWPENAKLYQPLKDDQILLSDHASSLAVQAYLRMCNLPVQVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFTKAKGHSLSDGLDEVQKAEMKAYMELVNNMLLTAELYIQWCDENTAMEITRPRYSSPYPWPLNHILAYQKQWEVRRKMKAIGWAGKTLEQVLEDMSQCCQALSQRLGTQPYFFNKQPTELDALVFGHLFTILTTQLTSDQLGEKVKNYSNLLSFCRRIEQNYFEDHDKDSLASSSSRFSKGPSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML72</accession>
    <name>W5ML72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein phosphatase 2 regulatory subunit B''alpha</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015361120.2"/>
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        <property type="nucleotide sequence ID" value="XM_069197298.1"/>
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    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT ALPHA"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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        <location>
            <begin position="1005"/>
            <end position="1040"/>
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            <begin position="381"/>
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            <begin position="604"/>
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        <location>
            <begin position="708"/>
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            <begin position="1145"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00093310"/>
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        </source>
    </evidence>
    <sequence length="1175" mass="131076" checksum="D0372EF0C56A9026" modified="2014-04-16" version="1">MAATYRIVVSTVNCYNSVVIDRRFQHTVHYCSGPCQVLKHGIECTVAHHSTCSELVEAPVAPASNARPSGAQELGNKFSAVENGNAAPEAMSEDYHQVLPGAPRIKKGLAIQGSTSLKDITGEAINLASGKIKEFSFEKIKYSSSHVTFRKGRKVRPDSFSRRSTDLDIIYGHFGNDENCPPFGMLQKSVMEEHKLSRSSSLEQNVNNVATLYLNSLTEENLINKILEKTKMDSNASGEDIKACLDILLKCSEDLKKCTDIIKQCIKRKSMGGESDDSSTNPELIYRNVMARLSSYLKKLPFEFEHGLPGRSEHNDLAELVNSLHGLQQSPFSPIFGNEQPPRYEDVVHSPPLTKTLPPSSLLESQEASREPVNNVKSVQSPSSLPSHPSSLANNVQQNTMTAANSSTSLPQVTAINHSDSYSSTNSLLSSSNDSICKESMEALYIEEDPDLGKVLDKELRGEGKFSLGFTEVKQFSKKPSTFAETSDGADKLSESISNGTTCKPKIDPHALQGASKAFVSPTNLSAESRAFQTLGHSNQEDDIDKLLMDLECLSQNMEQEPSMKGNSQQVVNCSQQPGSVQHLIFSSGSKSHPPPAMTSPLPGKKDTSRHGGDVNDEEDGALLLRILESIESFAQELVESGTGKGTLSREREVMRLLQDTLVTTAKSEQKSQPGPVPAVVRDTGPSLLIQQTPEVIRVQNKQEKKPGTPPPAPTSSTPSPRPPTPPPAHKVVVATSLSVNIPRFYFPKGLPNCSANLDETIAKIEAAFTEFEEEKADIYEMGKIAKACNCPLYWKAPMFNGTGGERTGFVSVHGFIATWRKLLLSCYDDASKFVYLLAKPGYNYLEQEDFIPLLQDVVDTHPGLTFLKDAPEFHSRYITTVIQRIFYTVNRSWSGRITMTELRKSNFLQTLALLEEEDDINQITDYFSYEHFYVIYCKFWELDTDHDLFIDQKDLARYNDHASSNRIIERIFSGAVTRGNSVQKEGRMSYADFVWFLISEEDKKNPTSVEYWFRCMDLDGDGVLSMYELEHFYEEQCERMEGMGIEPLPFHDLLCQMLDLVKPEQQGRITLHDLKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDADSEGGPEPSDWDKYAAEEYEILVAEESANEQLQEGSFEDDYESDELTVPPEIGNKMDKLVISDLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLX9</accession>
    <name>W5MLX9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">IKAROS family zinc finger 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Ikaros C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLX9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009388"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009399.1">
        <property type="protein sequence ID" value="ENSLOCP00000009388.1"/>
        <property type="gene ID" value="ENSLOCG00000007735.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158308"/>
    <dbReference type="HOGENOM" id="CLU_1329031_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLX9"/>
    <dbReference type="OMA" id="KKRHVCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007735">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000073">
        <property type="entry name" value="IKAROS family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000080">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000372">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN EOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="207" mass="23237" checksum="25598BFC4416AD3F" modified="2014-04-16" version="1">MICIGPNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCSYACRRRDALTGHLRTHAVSSPTVGKPFKCSYCGRSYKQQSTLEEHRERCHSYLQSLETHPAQSGQGEELRDLELMPDSLLHPSTERLTFIDRLTNSIAKRKRSTPQKFVGEKHMRLSVSDVPFDLNASFEKEGDLLGSQHVGTDPAGFVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMP5</accession>
    <name>W5MMP5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-3' exoribonuclease 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MMP5">
        <property type="interactions" value="1298"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009654"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009665.1">
        <property type="protein sequence ID" value="ENSLOCP00000009654.1"/>
        <property type="gene ID" value="ENSLOCG00000007944.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697761"/>
    <dbReference type="KEGG" id="loc:102697761"/>
    <dbReference type="CTD" id="54464"/>
    <dbReference type="eggNOG" id="KOG2045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00670000098080"/>
    <dbReference type="InParanoid" id="W5MMP5"/>
    <dbReference type="OMA" id="VASWPWF"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007944">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040992">
        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047007">
        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="13">
        <location>
            <begin position="652"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="14">
        <location>
            <begin position="848"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="12">
        <location>
            <begin position="1101"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1243"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1402"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1445"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1694"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1243"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1270"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1288"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1326"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1336"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1471"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1696"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1715"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1736"/>
            <end position="1754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1773" mass="200967" checksum="C6B52B842717A7DB" modified="2014-04-16" version="1">MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDEDVHFRISEEKIFADIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKALEKGEVLPTEARFDSNCITPGTEFMARLQEQLEYFVHNKISTEKSWQRVRVYLSGHETPGEGEHKIMEFIRSENARPDHDPNTRHCLYGLDADLIMLGLTSHEPHFSLLREEVRFGGKKSQKRISTPEETTFHLLHLSLMREYIDYEFSELRDKISFEYNLERIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYRTYISVLPKLGGYINENGNLNLANFEKYLEKLSEFDREHFSEVFVDLKWFETKVGNKYLNEAAGIAAEEAKSREGKRNKMLEDSLCLAALDENKDTEGLPTKDCVEEDGEDDDMFETEFRQYKRTYYMTKMEVEVVSDEFLAGQAECYVRAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIRNISKLEMRFEMAKPFMPFEQLLAVLPAASKDLLPECYRHLMTSEGSAIIEYYPLDFKTDLNGKQQEWEAVVLIPFIDEKRLLSAMEPYNAFLTKSEQERNRHSECAVYWYDKDTDFQYPSPLPGKFPDIVKCHVRHELISMDAWRVDPSYTRRKVDPGALYFCGFPTLKHIRHKFYKKKNGVQVFQQSSRGENMILEIIPEDNVEPECADVASSVLDKSVFVNWPHLEEARIVAVSDGETKFYLEEPPGVQKLYSGKTVPPTKVVYLSDKEQSIWLKEVQSIVEFYNRRKGIIINETAVLLYGQLMTGRKYVLDQKGGVHLEKQWAKQIIPFAYQTIVQDLKTFDSSVSCFKTLEELFTPGTTVFMLGNPYYGSVGEVLDSSDVISEGRFRVLFTVPCEPQLDALIHNQHKYSVKYSPGYVLASRLGITTYLVSRFSGSIFIGRGSKQNPHGEQKVNVGLNLKFNKKNEEVPGYTKKSGNEWLYSVAVEELLAEYLERFSEIFSYVSRNSHEDVFYEDDVWPGEDENGAEKVQEITSWLKTHPVSSSSRASCDLQILDTAIVEKIEEEVDKCKQKRSTKKVRVTVKPHLLYRPLEQQHGIIPDKDADYHLFDRVVNVRENFSVPLGLRGTVIGIKGADREDEILYEVVFDEEFVGGLTIRCSSGKGYRLPPSALINLTHGIRLEYGAQKLTAIVKPQPASASHQNSYFPASSLNPHKVQIGGLNHSPCSPFVPTQQLNGRQVYNLRADNQGNWHSQKTFNQKNTQKSHAKEDEFSSVWQSLQGSGAPQNPPALWQNSPTKSGWGAGSQSQAAVKGQHRQRDEGLHQQGQARRTKEEDRNQRNDTQQASRAQNKPVSSGAGRTKASIRVLKRNEDLNAVDSAQPHSSSQDTSNKVSTEFEELIANLKISKGGGTLPSQTKDQIHTTEEPLSPQSFAMKGTLMLKEMLKIDGSSSADSEKVKPSPVEPVASATTTSYQPRRRPSKKLAAHINKPNVPGVAQHGPGADKPGQCLPPHQPLIPTVASELSRICLGLGMSVPEFAFLRTPQGMTICQVKLSNGLLVHGPQCQSESEAKEKAALFALQRLNSVGSGFPLAQPMFSGMQQMRATLPSGPVHSVFGQPPGNLLMHPPAHGYGPLHWGAPVQMQGQAFYQGTYPGARPPAPAVPIGTHNQFVPLQVTKKRAAGKKNLEAREYYNSQYVRSQKSEQTVKPEACDPSFQRITAPPATVTPSQTPPTSQEPQERSTSSTLKQNPNPQTTGSSAKRKPRKLAVNFDAAKVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNJ7</accession>
    <name>W5MNJ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01020776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNJ7"/>
    <dbReference type="FunCoup" id="W5MNJ7">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009956"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009968.1">
        <property type="protein sequence ID" value="ENSLOCP00000009956.1"/>
        <property type="gene ID" value="ENSLOCG00000008200.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159191"/>
    <dbReference type="HOGENOM" id="CLU_020283_0_1_1"/>
    <dbReference type="InParanoid" id="W5MNJ7"/>
    <dbReference type="OMA" id="FCFHIYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008200">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF8">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="121"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="357"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="526"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="583" mass="63908" checksum="3B8D935B07F02FAF" modified="2014-04-16" version="1">MNICNRNPNRTAPEKEGGPSCPELPQCSRTNGWSWPPHPFQLLAWLLYVYFAVIGFGVFVPLLPSHWIPAGYICTGVMFVCHLCVHLTAVSIDPADRNVRAKSYRGPVPVFDRSKHAHVIENCHCYLCEVDVSPKSKHCSACNKCVASFDHHCRWLNNCVGSRNYWLFLNSVISALLGISLVVLIAFYVFVEFFLDPSQLRSDKHFQESQNESVVWFVFLPVAPVSTAGPAIPALAAITIAVGLLSVVLLGHLLCFHIYLMWNRLSTYEYIVRQRHRQETRDLDREQVGQDSVPPKLQPIKKNQDIVYSGTLGYTNPEIEVEDPATMVSTEKEFYGNGVVRTLASRKAEEDLLPTISVEHQQPPCSHRRSQKKTRKKVHRVPVELASDRSVENSAGRAPAGSPSEPEPCVGAATVVTPLSQRLPLPAFPPRAALPLLAPVQAAGPPAEYHSDSAESLEEIPVAQARLGSAAMAGYSSRGDPGATSTQQTLQPSIGSHARLPSPQPSSKRREPRRPLAQQRFELLPHTPSVFMSRSSGQPGVPPGPVEAPTSHGKRKRNSRKGPEGPGDACNRTDAALARGSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPJ7</accession>
    <name>W5MPJ7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulatory factor X, 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RFX family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPJ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010306"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010319.1">
        <property type="protein sequence ID" value="ENSLOCP00000010306.1"/>
        <property type="gene ID" value="ENSLOCG00000008459.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244970"/>
    <dbReference type="InParanoid" id="W5MPJ7"/>
    <dbReference type="OMA" id="SKYYCYG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008459">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000128">
        <property type="entry name" value="DNA-binding protein RFX5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF18">
        <property type="entry name" value="DNA-BINDING PROTEIN RFX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18326">
        <property type="entry name" value="RFX5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="6">
        <location>
            <begin position="91"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="258" mass="28844" checksum="6FFDE8EB6385469F" modified="2014-04-16" version="1">MADDGPQAASSKRGGLPAPEGDTEPNMLLQKLKSSISKNIQTKVDSILQDVQRFSDNDKLYLYLQLPSGPNAGEKSSSDSSSFSTADQLHTCNWIRSHLEEHADTCLPKQDVYETYRKHCENLQYRALSAANFGKIIRDIFPNIKARRLGGRGQSKYCYSGIRRKTVLNMPLLPNLDLKNDPDFPLPPAQSELTELVQTYNQEVTEAACELICDWAEKILKRSFDTVVEIARFLVQEHIVNPRCSQAELITSAALAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ27</accession>
    <name>W5MQ27_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-crystallin A4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-A4 crystallin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ27"/>
    <dbReference type="FunCoup" id="W5MQ27">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010486"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010501.1">
        <property type="protein sequence ID" value="ENSLOCP00000010486.1"/>
        <property type="gene ID" value="ENSLOCG00000008625.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTF8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160372"/>
    <dbReference type="HOGENOM" id="CLU_081883_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQ27"/>
    <dbReference type="OMA" id="EFTSECY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008625">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF19">
        <property type="entry name" value="BETA-CRYSTALLIN A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="220" mass="26246" checksum="53D2E7EBDEC984C1" modified="2025-10-08" version="2">MSPCPEPSASRCPCPRPLWRPLRDKMTHHCTKFSGHWKIIVWDEECFQGRRHEFTSECYNVMEFGFETVRSFRVESGAWVGYEHSAYQGQQFVLERGEYPRWDAWSGSNAYHIERMTSFRPIACANHRECRMTIYERENFLGRKGELSDDYPSLQAMGWCNNEVGSFRVQSGAWVCYQYPGYRGYQYIMECDRHCGEYKHWREWGSHAQTFQIQSIRRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQE3</accession>
    <name>W5MQE3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639216.1">
        <property type="nucleotide sequence ID" value="XM_006639153.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQE3"/>
    <dbReference type="FunCoup" id="W5MQE3">
        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010602"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010617.1">
        <property type="protein sequence ID" value="ENSLOCP00000010602.1"/>
        <property type="gene ID" value="ENSLOCG00000008712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696283"/>
    <dbReference type="KEGG" id="loc:102696283"/>
    <dbReference type="CTD" id="30321"/>
    <dbReference type="eggNOG" id="KOG3802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164385"/>
    <dbReference type="HOGENOM" id="CLU_013065_1_2_1"/>
    <dbReference type="InParanoid" id="W5MQE3"/>
    <dbReference type="OMA" id="PPHQGHW"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008712">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF125">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="13">
        <location>
            <begin position="240"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="330"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="332"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="440" mass="47188" checksum="357E395C30083E11" modified="2014-04-16" version="1">MATAASNPYLPSNSILSSGSIVHSDSGGGGMQPGSAAVTSVSAGYRGDPTAKMVQSDFMQGAMAASNGGHMLSHAHQWVTTLPHAAAAAAAAAVAAAEAGSPWSSSPVGMTGSPQQQQDVKNNSGRDDLHTGTTLHHRPPHLGPHQSHPGAWGGTTASHIPSIAGGQQQQSLIYSQPGGFTVNGMLSPPPGSQSLVHPGLVRGDTPELDHGNHHHHHHHQHQHHQQHHGGVNSHDPHSDEDTPTSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSPTSIDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITTLADSLQLEKEVVRVWFCNRRQKEKRMTPPGVQQTPDDVYSQVGNGHFLVDYLKDASQPSDQRVTTTSSFHQVILAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQF0</accession>
    <name>W5MQF0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the riboflavin transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQF0"/>
    <dbReference type="FunCoup" id="W5MQF0">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000010624.1">
        <property type="protein sequence ID" value="ENSLOCP00000010609.1"/>
        <property type="gene ID" value="ENSLOCG00000008715.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003774"/>
    <dbReference type="HOGENOM" id="CLU_034789_1_0_1"/>
    <dbReference type="InParanoid" id="W5MQF0"/>
    <dbReference type="OMA" id="CTWIGTN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008715">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52, RIBOFLAVIN TRANSPORTER, MEMBER 3-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="50181" checksum="291B46963B858293" modified="2014-04-16" version="1">DRRSAMSLLTHALACLFGMGSWVAINGMWVELPLIVPEIPEGWYLPSYLTVLIQLANMGPLFVTLMHRFYPGKLSEVAVIYTIISLGILATLLLAFFWKETLVIGGDSRSVPLLVLTFFLSLVDCTSSVTFLPFMMRLKPQYLTTYFIGEGLSGLVPGLVALIQGVGVVQCVNQTHAINETWNSTTDTFELQAWYQPANFSPQIFFIFLSFMMGVCLVAFLLLNYHPAVSRECKHNRYLDGVANGENRELAHRRNNQPEQKPMISPLESPQSELKSSFGSGKYSWMQVLFVFVVLAWVNALTNAVLPAVQSYSCLPYGNSAYHLAATLAAVANPAACFIAMILPLRSLLLMAGLTTFGTGVGCYIMATAVLSPCPILVHQSTGTFLIVIAWVLLVLTLSYVKVMIGVILRDEGHSALVWCGAVVQLGSMLGALTMFPLVSVYNFFRSGDPCNTKCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRJ8</accession>
    <name>W5MRJ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="19">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer. Associates with late pre-40S ribosomes. Interacts with CEP78; promoting deubiquitination of tektins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01013894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015197535.1">
        <property type="nucleotide sequence ID" value="XM_015342049.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015197536.1">
        <property type="nucleotide sequence ID" value="XM_015342050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRJ8"/>
    <dbReference type="FunCoup" id="W5MRJ8">
        <property type="interactions" value="1515"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011007"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011023.1">
        <property type="protein sequence ID" value="ENSLOCP00000011007.1"/>
        <property type="gene ID" value="ENSLOCG00000009029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695451"/>
    <dbReference type="KEGG" id="loc:102695451"/>
    <dbReference type="CTD" id="10600"/>
    <dbReference type="eggNOG" id="KOG1873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156013"/>
    <dbReference type="HOGENOM" id="CLU_007938_1_0_1"/>
    <dbReference type="InParanoid" id="W5MRJ8"/>
    <dbReference type="OMA" id="MAAGHYV"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009029">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02667">
        <property type="entry name" value="Peptidase_C19K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000147">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000045">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
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        <property type="entry name" value="USP"/>
    </dbReference>
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        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF852">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
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    </dbReference>
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        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="USP_2"/>
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        <property type="entry name" value="USP_3"/>
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    </dbReference>
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        <property type="entry name" value="ZF_UBP"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 19" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="22">
        <location>
            <begin position="22"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="21">
        <location>
            <begin position="209"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="410"/>
            <end position="558"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="419"/>
            <end position="430"/>
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        <location>
            <begin position="448"/>
            <end position="467"/>
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    </feature>
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        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="530"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022670"/>
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    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022807"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="RuleBase" id="RU366025"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="824" mass="91879" checksum="F5961AA089D8007E" modified="2014-04-16" version="1">MGKKRAKEKHLRDEDPVDVTGPNCRHIRKGIDSSLLKKTVQDVNWNACQDCKGEEIPASTSAEENSEGPGIWFCLKCGHRGCGRHSENQHAFKHYEKPHSDPHCLVLSPDTWSVWCYMCDDEVQYSRTGQLAQLVNNIRKQALSEMTKKTLTKTVIDLEIPKETLNDTTSEENKNGQKKDTKADIAGKKEEKLSLKKAPQTNSNSLAVKGLSNLGNTCFFNAVLQNLSQTQFLRQLLNEMKDEKTTLQITPHLSSQLEPLVINLDRPGSLTLAMCQVLNEIQETKKGVVTPKELFSQVCKKAARFKGFQQQDSQELLRYLLDGMKAEEMKRVSSGILEALNNSGKLIEAEESKKLVKEYEKNGAPRNFVDQVFGGELTSTVMCKECKTVSLVTEMFLDLSLPVSDEAYRKKSTKKVVRRTSDNEEGDSKKTSVSPAEIEELPSGSKYQQKKAKKQAKKQAKIQRRQQKQAGKLGLGSTSSQDIEHVNGDESAAQEEIDQSELGATTQGEACLESSEPNNAPPSNSQNSKQPDHCDEETHSGEEAQPEDCEPSESCSDNRFKALDNKEGLGEDELVKDMNSLTINEDFISPDDIDFEIDSCGDAVDTKAYTVVDQDPELAFTTLADREAPGKQDCSVQSCLYQFTEVEHLTTNNSLQCVTCTQHKNKGSRSSSDGEKCVYTDALKQMLISLPPPVLTLHLKRFQQVGYSVCKVNRHVIFPQVLDLAPFCTTNCKNVPEGEKKVLYRLYGIVEHSGTMRSGHYTAYVKVRPNNCLTNQLNGLTPQASDSESVKGSWFHISDTSVQAVPETKVLNSQAYLLFYERIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRK6</accession>
    <name>W5MRK6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MTSS I-BAR domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRK6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011015"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011032.1">
        <property type="protein sequence ID" value="ENSLOCP00000011015.1"/>
        <property type="gene ID" value="ENSLOCG00000009035.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRG4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183156"/>
    <dbReference type="InParanoid" id="W5MRK6"/>
    <dbReference type="OMA" id="QKKAKKX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009035">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034334">
        <property type="term" value="P:adherens junction maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07643">
        <property type="entry name" value="I-BAR_IMD_MIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22060">
        <property type="entry name" value="WH2_MTSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000010">
        <property type="entry name" value="Metastasis suppressor 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF10">
        <property type="entry name" value="PROTEIN MTSS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="742"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="696"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="770" mass="83507" checksum="44C5DDDBA4A0C6A0" modified="2014-04-16" version="1">MKSSYPIWEDFINKAGKLQSQLRTTVVAAAAFLDAFQKVADLATSTRGGTRDIGSALTRMCMRHRSIEAKLRQFSAAFIDCMINPLQDQMEEWKKVANMLDKDHAKEYKKARQEIKKKSSDTLKLQKKAKKGRGDIQPQLDSAMQDVNDKYLLLEETEKQAVRKALIEERARFCAFVSMLRPVVEEEISMLGEITHLQTISDDLKALTMDPHKLPPASEQVIIDLKGSDYKWSYQTPPSSPSTTMSRKSSVCSSLNSVNSSDSRSSGSHSHSPTSHYRYRSSTLPQQAPVRLSSVSSHDSGFISQDAFQSKSPSPMPPENIPQTSSSSASSDASETCQSMSECSSPTSVSSGSTMGGAWASAEKLSEGYDHYSLCTDPPFLAATGSAGQFPFLPPSSSRSGTRPGWALLPDYPHYCTLGPGMLLSSKIPSWKDWAKPGPYDQPMVNTLQRRKEKREAADAGAPAAHTGWPHHFNQPKPVQLWECATMFVCQQGEEMEAHEELALVLTRGLQLDTQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSEDQEVEQQDFDKSSTIPRNSDISQSYRKMFQAKRPASTAGIPSNAGPVMVTPGVATIRRTPSTKPSVRRATVGAGPIPIKTPVIPVKTPTVPDVPGGFPSSLGVSSGEGIEQVTTPESPMAPVVVEGEPGGGVVVSSWSGKASINPPVPPPQPSSGVEFNSGIKQATPGEETLSEDSAQDTALPDVQGDNMLLAIRRGVKLKKTTTNDRSAPRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRP1</accession>
    <name>W5MRP1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Metal transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ACDP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRP1"/>
    <dbReference type="FunCoup" id="W5MRP1">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011050"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011066.1">
        <property type="protein sequence ID" value="ENSLOCP00000011050.1"/>
        <property type="gene ID" value="ENSLOCG00000009070.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157525"/>
    <dbReference type="HOGENOM" id="CLU_011310_1_1_1"/>
    <dbReference type="InParanoid" id="W5MRP1"/>
    <dbReference type="OMA" id="NSIMCSR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009070">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000001">
        <property type="entry name" value="Putative metal transporter CNNM3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057492">
        <property type="entry name" value="Ig_CNNM1/2/4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF28">
        <property type="entry name" value="METAL TRANSPORTER CNNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25562">
        <property type="entry name" value="CNBH_CNNM2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25511">
        <property type="entry name" value="Ig_CNNM4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="16">
        <location>
            <begin position="194"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="461"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="727"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="776"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="828"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="727"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="796"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="828"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="874" mass="97633" checksum="CD93A94258BE6806" modified="2014-04-16" version="1">MSSYNTFSIMAADAAAAGAPTCILRPGSLPLLLIFAISLPASALGLLGFRPEDTGGELSVEDGVLKATEGTRFRLRVYYAASQQRLNRSVSGRAGDAATAPWIAFIEEPSPGREGQVHPKRNLCVDKNARTTDIELLGSFKSASSQNSILVELLAKDLRKGEKIKYYSMCAFDGIKWEHFRTRDFWLAVVERSPEPDVWLQVIVSLLLLGFSAVFSGLNLSLLALDPVELRVIQNSGTDKEQKHARRIQSVRKHGNYLLCTLLLGNALINASLAVWMSHIFGVTWVSILACTVGIFFVGEILPHSVASRHGLAIASKTIWVTRLLMLISFPISYPISKLLDAVLHQEISSFYTREKLLEMLRVTDPYHDLVKEELNIIQGALELRTKTVEDVLTPLNDCFMLASDAVLDFNTMSEVMQSGYTRIPVYEDERSNIVDILFVKDLAFVDPDDCTPLKTITHFYNHPLHCVFNDTKLDAMLEEFKKGKSHLAIVQRVNNEGEGDPFYEVMGIVTLEDIIEEIIKSEILDETDLYTDNRNKRRVSHHERKQQDFSLFKLSENEMNVNVSPQLLLATHRFLATEVEPFKPSYLSEKILLRLLKHPNVVQELKFDERNKRAAQHFLYQRTKPVDYFVLVLQGRVEVKIGKEALRFENGAFTYYGVPAIVPALSSVHRSPSRSSGLNHSDSTIYGGSMSQLNTSTTTYLPDYSVRQLTDLQIIKITRSHYQNALTASRIDSSPQTPDTDAQMNEDNTPTPEVPVPEARPYSIALPLPEPRLSAAARPRPHAGGTQHSTTLLNEKNRIVRSKSDGQKSPSDSVFLRMDDIPYIKEDLTGNGKYADRDAIPDGADHPTSPFISSLSLSGSDETLGKKLLSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSC5</accession>
    <name>W5MSC5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TBC1 domain family member 31</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Molecular adapter which is involved in cilium biogenesis. Part of a functional complex including OFD1 a centriolar protein involved in cilium assembly. Could regulate the cAMP-dependent phosphorylation of OFD1, and its subsequent ubiquitination by PJA2 which ultimately leads to its proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSC5"/>
    <dbReference type="FunCoup" id="W5MSC5">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011284"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011300.1">
        <property type="protein sequence ID" value="ENSLOCP00000011284.1"/>
        <property type="gene ID" value="ENSLOCG00000009250.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153859"/>
    <dbReference type="HOGENOM" id="CLU_003330_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSC5"/>
    <dbReference type="OMA" id="GCYPEKY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009250">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000185">
        <property type="entry name" value="TBC1 domain family member 31 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000022">
        <property type="entry name" value="TBC1 domain family, member 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003385">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF1">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="14">
        <location>
            <begin position="426"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1033"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="816"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="908"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1033"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1078"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014199"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1089" mass="125956" checksum="01134B12CAC035B5" modified="2014-04-16" version="1">MQTTDIGTKECGKIWYRKPTPSPSDGVLIRVVRSAAGYHSKTVRFLQAAFDTTGEAFLAGDHHGNTYVFDISRNRFRLVQKTGQACTALAFNLRRKTEYLVALADYSIKCFDTDSKELVSWMRGHESAVSSVSVHGSGRYAITTSADTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLYCKYQLPVPEEGPRLHYKAFAVTRDGRTLVAGGKSNLLHLWCLENRQLLRIIQMPPKVRTVRHLEFLPDSFDGGSSQILGVLSQDGIMRFINVQTCKLTFDIGSHDDGISSAAISPNGHHIVAVMENGSLNIYSVESLTQDINKPPPPLVKVISGFERGSGVSKDAGLRMQVKSEHVQRPVRSAGGKTQTRVLRPPADPTPEDKENELPDGLSRKRLQALLKEFGEYPAKYRMFIWRSLLRLPENHAAFSSLAAKGPHSAFLRLHEHYPIKSQKLQRVLQRVLSALAHWSAIFGETQYLPLVAFPFVKLFQNNQLICFEVIATVIMNWCQHWFEYFPNPPLNVLCMVENVLAQHDKELLQHLVNCGVTSQLYAWPLLETLFSEVLTREEWLQLFDNVFSNHPSFLLLAVVAYITCCRSPLLHCTLTEDFEYFFHHRNNLDISAMIKETYRLMDSTPAELHPRHMLCDFEALTKGQYPVFNKYPTFIVEYQSQERERIRQQEMEYLRERQMAQELRSEAVRRQAEDEAWHRQQELLQEAEEHRRRMLLEEESKLAEQRARLVAMKRELKTKELHLIDAARRRFIKYQQDQKKMELRRLDDEINRKASMREQETATVLQDIEVRQMELEAQRRLFEQQLAKEQERVNQEVKGEVDTHRRKAEAEDHAFQKLVDAEKDQDLETKRVLEESWAEADQLCTELGWQAEVRRRLEQADAERGRCHLELARLNRETRAEEVQLLQLMKEMEGKRWEDVVERRAQLEEEKQAAASAEAQRKAFLTAEKEEAEHLADSLRQSQEESLERLRELRVRSPCSVRGLLPKPNTDQSEPRNTCLNILSSDDSSTHFSLDRGRSDLESRERELMLDVQELRQKIAAQARNTKPPDRSSKISFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTA7</accession>
    <name>W5MTA7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">T cell immunoglobulin and mucin domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTA7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011633.1">
        <property type="protein sequence ID" value="ENSLOCP00000011616.1"/>
        <property type="gene ID" value="ENSLOCG00000009509.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RY2T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163509"/>
    <dbReference type="HOGENOM" id="CLU_047504_2_2_1"/>
    <dbReference type="InParanoid" id="W5MTA7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009509">
        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060097">
        <property type="term" value="P:cytoskeletal rearrangement involved in phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608:SF2">
        <property type="entry name" value="T CELL IMMUNOGLOBULIN AND MUCIN DOMAIN CONTAINING 4 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="220" mass="24134" checksum="C305CD6347EFA127" modified="2014-04-16" version="1">PRSSAAGYRALEGGAVSLACRYSVKRYGLSRVCWGRNCGTLWCSDTIVQTDGSRVISKVSDRYRLNGDIPAGQVDLTISRVRQADSGSYCCRVDIDGYFNDQKVSHTLQVLKGRLHQPWATQILNTDVAVRNSQLINQISHQTVEARGRTGLNWNGVILSQMTSCDRGSALPMRHGQVLEESAIVTQSLQINVPVLAGSITVLLFLLLASSLALLGFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU53</accession>
    <name>W5MU53_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thyroxine-binding globulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Serpin A7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">T4-binding globulin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Major thyroid hormone transport protein in serum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU53"/>
    <dbReference type="FunCoup" id="W5MU53">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011912"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011933.1">
        <property type="protein sequence ID" value="ENSLOCP00000011912.1"/>
        <property type="gene ID" value="ENSLOCG00000009755.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160877"/>
    <dbReference type="InParanoid" id="W5MU53"/>
    <dbReference type="OMA" id="TLMSFQH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009755">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19549">
        <property type="entry name" value="serpinA_A1AT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000003">
        <property type="entry name" value="alpha-1-antitrypsin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.310.10">
        <property type="entry name" value="Serpins superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF375">
        <property type="entry name" value="THYROXINE-BINDING GLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00780">
        <property type="entry name" value="LEUSERPINII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868288" description="Thyroxine-binding globulin" evidence="12">
        <location>
            <begin position="21"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="13">
        <location>
            <begin position="77"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="439" mass="49594" checksum="1DC62FEF6F5FC196" modified="2014-04-16" version="1">MGKGIYFFITAALVCSAVQGNHHHHSDDDHAHGKGHHHDHGHHHDHGHHKGHGHHHGHKHHHNYSLEVQSANAKFAFSLYKHIAAQPDAQTKNIIFSPLSISTALAMLSLGAMGKTHNELFQGLGFNKTDTTEAEVNEAFEQILQSLNQKKELDLSVGNAMFIRESFKPLSKFLEDIKHYFHSEGFTVDFTNTTAALHLINKYVEDKTHGKVPHFLDDLNADTLMFLVSYIYFRGKWEIPFDPRDTSQDIFHVDDNTTVPIQMMHKDGSFDVYHDKESSTHVLRLGYKGGVSMILLLPEREVKDLEESWCREHLRKWHRSAKKQRVSLSLPKFSLSTSYSLKDILSEMGMKDMFERSADLSGISKDNVFVSKVDHKATLDVDEVGTSATAGTGIQILLGSSLFPPPLTLTFNRPFHFVISNHETRSILFMGKIVNPTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUD4</accession>
    <name>W5MUD4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="2">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01001112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200534.1">
        <property type="nucleotide sequence ID" value="XM_015345048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUD4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011993"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012014.1">
        <property type="protein sequence ID" value="ENSLOCP00000011993.1"/>
        <property type="gene ID" value="ENSLOCG00000009818.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689984"/>
    <dbReference type="KEGG" id="loc:102689984"/>
    <dbReference type="eggNOG" id="ENOG502S1HV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017291"/>
    <dbReference type="HOGENOM" id="CLU_067834_0_1_1"/>
    <dbReference type="InParanoid" id="W5MUD4"/>
    <dbReference type="OMA" id="PACNRMM"/>
    <dbReference type="OrthoDB" id="10028716at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009818">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF9">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="291" mass="32521" checksum="A912CBCFF6213113" modified="2014-04-16" version="1">MGTAERGMAMERQGCCAILNTKRVTVIICVLILLTLAIAGFAVLIRTTETGGSLKYVFITRCKEYLETHDEVRVGIDCNEVWDAFQSSFVAKDPCKISVEAYDGLMNTAKQKVPCNEMVFWSKTKQMAHKFTQAKKCFMTLEDTLLGFIMDGLTWCGSQDGNDTYTSGCPNWNTCEQNPVRSFWSRASAHFAESACGNVTAMLNGSIDKPFSAKSIFGSVEIKKFNPMKINYVNVVLIGRENYPMSCKNDSLQELEKTLIPKFKYTCTTVLLSTILKCLEDPQKPCGPCWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVB4</accession>
    <name>W5MVB4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aminopeptidase B</fullName>
            <ecNumber evidence="12">3.4.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Arginine aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Arginyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.</text>
            <dbReference type="EC" id="3.4.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.6" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01014279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVB4"/>
    <dbReference type="FunCoup" id="W5MVB4">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012323"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012344.1">
        <property type="protein sequence ID" value="ENSLOCP00000012323.1"/>
        <property type="gene ID" value="ENSLOCG00000010074.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682358"/>
    <dbReference type="KEGG" id="loc:102682358"/>
    <dbReference type="eggNOG" id="KOG1047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160431"/>
    <dbReference type="HOGENOM" id="CLU_014505_2_1_1"/>
    <dbReference type="InParanoid" id="W5MVB4"/>
    <dbReference type="OMA" id="QLMDLDD"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010074">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000011">
        <property type="entry name" value="Arginyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000003">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF1">
        <property type="entry name" value="AMINOPEPTIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="19">
        <location>
            <begin position="528"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="324"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="628"/>
            <end position="630"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="679" mass="77392" checksum="1D273E6BFC5E57F6" modified="2014-04-16" version="1">MKASISAGVSKRLVVTCCHSRLHSRLQTRQTGRTQCYRIAAPRVVKLREMEESLSLHSDRAEDVASDSSYRHFRIKHFHLDLEVDFEQRQLRGTETLQVRCLRDSQSELRLDVHPSLRLQEVSFLGVGGTEWTAAQFETRGFTSYGTTLVLRFPNPWKSKDEFQVAIKYCATDGPGVCWLEPEQTAGRTKPFVFTQGQAVLNRSFFPCCDTPAVKSTYSASIKVPEGFTAVMSATTWQHRKADNVFLFTMEQPIPSYLVALAVGELVSAQVGPRTRVWTEPCLLPAAQQEFDGVIEEFLAVGEKLFGPYMWGRYDVLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLAGVIVHEICHSWFGNLVTNANWGDFWLNEGFTMYAQRRVCKEIFGEAYTCLEAATGRALLRQHMDNTGQQHPLNKLRVKIEPGVDPDETYNETPYEKGFCFVSYLAHLTGDQSRFDSFLRAYVDKFKFRSVVAEDTLEFYLEYFPDLKEKDVHRIEGLEFDRWLKVPGWPPYLPDLSAGQSLMKPAKSLAALWAEEQLDVVAIAAIDPVPWRTYQTVYFLDKVLEKSPLPTGHLERFAQQYPHIMGSHNAELRLRWAQIVLKNQYEPGFQHVRAFLSCQGKQKYTLPLYRAMWSGSEKTRALAKEIFSATSEQLHCIVRSYIQKILDNPAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVG4</accession>
    <name>W5MVG4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin specific peptidase 40</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVG4"/>
    <dbReference type="FunCoup" id="W5MVG4">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012373"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012394.1">
        <property type="protein sequence ID" value="ENSLOCP00000012373.1"/>
        <property type="gene ID" value="ENSLOCG00000010114.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157267"/>
    <dbReference type="InParanoid" id="W5MVG4"/>
    <dbReference type="OMA" id="PEGSHWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010114">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039021">
        <property type="term" value="P:pronephric glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000043">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057763">
        <property type="entry name" value="UBL_USP40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF842">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25822">
        <property type="entry name" value="UBL_USP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="42"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1241" mass="138714" checksum="E7C20DAF11E2B57E" modified="2014-04-16" version="1">MFGNLFEEDDAAFVPQSTSSDGRKCQGKNSEPPPPRGMVNLSGIRNQGGTCYLNSLLQTLLFTPEFREVLFSLGPEELGYLSDKDKPEAKVRVIPLELQRLFAYLLLVDQQAASTADLTDSFGWTSNEETSQQDVQELNRILFSALESSLVGTSGSDIISRLYHGTVVNQIVCKECGNVSERQEDILDLTVSVKGLSGLLEALWSMFVEEEFFEGNNLYRCSCCGNLVTAAKSAKLRKLPPFLTISLLRFSFDFTKCERYKETGRYTFPVRMNLRPFCEQAERADSEYEYELFSVIIHKGGCYGGHYHVYIKDLDQLGNWESPEEEAKPKLPKKDVKAPDSELEVEDPLAVLKAILSQEESKTILVDQLGQKLLDKIGVSWNKKFRKQHGPIGKFLQSHADVFLLVSNGTQVQLKPNGLEAVEPSQKADPVTPERTEVPVPGDEHHWFDLNDTSVQPIREQDIEKQFQGKESAYMLFYRKAELRRPAEARKNPRYKVPVHLLQETEEENKKLQRMRSEFEESSNTIEVRLHLAPHYKVENGALHPSSSPQDSVLPLAFDRRKTVGDLRQAIYQVRVHSWEGDMALTVVKSLPAGLHLYDTLIDDQQSLYSSGIADGMDLFVWNGQEVGGVNVQTGADWEPVLLNLVRPSVGGEDPGLSEAPQGFSGGLELGAVRQALGKEELLLCQEEKGLGTHGSGWRVFPPADMHKTIKELALRDGDSILVLEPQALDLSQFTLNGDIVAVTTPSECRWLQVAPWTGEAAGEGDGKAVKVPASGDMLLSEVKLKAMEELCLQDQLLGSECCLRQADNSGKLLPPVREDMSIREAGVKLMSTLSLCPGPAPGPHQLFLYFTVGTDPPAGPEMEIIVEDTVTVKECLKLMLKRAGLEGECWHLRKVDWCYEIGEPLNEEDTTLAKLKVRSGDTLVVTEGRLPPKGFLRLPVWLYGEPSISLSQQDPQINHVAEQIGELSLGTSTAEHLYPSVDALELHLQFMGLVEISGEASLEDLKMQVMTLPNFQNLCVPSSEFLRVWVLEGKRPAKILRGNQQPINTLKLGSGTEICVQRLCKEENLGVNTIVLYVKMGVPAQRQYYPAEELVWDTAKECTARALRQAIASHYALPLDKVEVAKYFPDKYEWMVVSSWSQQVSKRKKKKKLENLLGAPFHLKDGDVIGVKNLLIDQNKDFSTLRDDIGKQQLRQEVENKRNSGKRVSFQNAEPYPERKPSGRNRKPEVALSINVGVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXD9</accession>
    <name>W5MXD9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 5 member 8, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXD9"/>
    <dbReference type="FunCoup" id="W5MXD9">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013048"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013076.1">
        <property type="protein sequence ID" value="ENSLOCP00000013048.1"/>
        <property type="gene ID" value="ENSLOCG00000010641.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690258"/>
    <dbReference type="KEGG" id="loc:102690258"/>
    <dbReference type="CTD" id="556156"/>
    <dbReference type="eggNOG" id="KOG2349">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165906"/>
    <dbReference type="HOGENOM" id="CLU_018808_11_1_1"/>
    <dbReference type="InParanoid" id="W5MXD9"/>
    <dbReference type="OMA" id="GFFSHTM"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010641">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005343">
        <property type="term" value="F:organic acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015730">
        <property type="term" value="P:propanoate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF25">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="306"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="66652" checksum="38AD56742192D872" modified="2014-04-16" version="1">MVGTGGPVALFSVWDYVVFVAIVLGAAAIGLYQAFSGRTQSSSSEFLLGGRQMTAVPVAMSLTASFMSGITVIGTPAEAYRFGAAFWLFAFAYTIMSTITAEIFVPVFYRLGITSTYEYLEMRFNRLTRIIGTTMYIFQTALYTGMAIYAPALALEQITGLNLWGVLVATGTVCIIYCTLGGLKAVIWTDVFQMVVMLAGFVAVIARGMVVTGGLHKIWNDSYNGGRLNAWDFDPDPLKRHTFWTIVVGGSLMWTSMYAINQSQVQRYISCQTMVHAKLSLYVNMVGLWVTVTLAMISGLTMYSIYKNCDPLTNGDIGAPDQLLPYLVMDILADFPGVPGLFVAAAYSGTLSTVSSSINALVAVTVEDFIKALWPRTSEKLLFWISMGLSVFFGALCIGMAALSSLMGGILQAALSIFGMISGPLLGLYLLGMLFPCGNTTGGLTGLITSLVLTLWVGIGAQIYPPLPEKTLPLSLSTDGCFSNLTQHLNGSTTTPWSIGGTPTAASYSVERPAIADNWYSLSYLYFCPLGTLVALIFGLFISIISGGCKQKKVNPDLLIRKSDLICFTCNKESPQLSVAEWKEKSDGNLAKGISNPAFKNTERNIQIIMEKMTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXH7</accession>
    <name>W5MXH7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NudC domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021406">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="17"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="160" mass="17738" checksum="8E21CE2BEBFB104F" modified="2014-04-16" version="1">APGMSVHFDERSGVIPCKTAWGAWYQTMEEVFIEVDVPPGTSAKDIKCNLGSKQIELAVKGRDVFKGKLFGSTIADEGTWTLEDKKLIRIILMKSNREAGNCWPSLLEGEYCADPWVQDQMQRKLTLERFQRENPGFDFSGAEISGNFAGGGPDFSNLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY07</accession>
    <name>W5MY07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBF4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY07"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013266"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013294.1">
        <property type="protein sequence ID" value="ENSLOCP00000013266.1"/>
        <property type="gene ID" value="ENSLOCG00000010827.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXIX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000037606"/>
    <dbReference type="HOGENOM" id="CLU_895860_0_0_1"/>
    <dbReference type="InParanoid" id="W5MY07"/>
    <dbReference type="OMA" id="SHPAANE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010827">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038890">
        <property type="entry name" value="ZDBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21639">
        <property type="entry name" value="DBF4-TYPE ZINC FINGER-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21639:SF5">
        <property type="entry name" value="DBF4-TYPE ZINC FINGER-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35639" checksum="CF34EB49E52065EE" modified="2014-04-16" version="1">MKVCIDLEDPKYNTQLNCALNLRSETERSHENLKGEDTVELKEHNDDKDVLPPLPHIPQSFVGKTWSQVMHEDDLKIEALVREFREGHFRCYFDSESLAKFGRKSKTRRNKDEATRETLPKSNLVTGTAEALKKKAGRRMWRLASRCQVVKVSHSTQTPTLSCPVIKTKAPTKDNELRDAKEVPNGSGWEKTPEMKTRLCALKLPETYSKIMSPVQPKTVVYVLSSPESGQCTLKPLTIRRTGRKRKSTESESALKYKYKKTPLKYYDPLTNRILKMPPKGIGVCRPKKFPHVRQLFRSLSPDINKEKQCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0K6</accession>
    <name>W5N0K6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Regulator of G protein signaling 9 binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RGS7BP/RGS9BP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0K6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014165"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014194.1">
        <property type="protein sequence ID" value="ENSLOCP00000014165.1"/>
        <property type="gene ID" value="ENSLOCG00000011529.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153725"/>
    <dbReference type="HOGENOM" id="CLU_634519_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0K6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011529">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16555">
        <property type="entry name" value="RING-HC_RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026512">
        <property type="entry name" value="RGS7BP/RGS9BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047986">
        <property type="entry name" value="RNF182_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21029">
        <property type="entry name" value="R-SEVEN BINDING PROTEIN (R7BP) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="432" mass="47793" checksum="F6F36BCFED1A3306" modified="2014-04-16" version="1">MVDSCCAEELECKICYCHYNLGNRRPKVLQCCHRLCAKCLVKILDLGESPPNAIVCPFCRYPTSLPEEAEGSLPDDYNIVQALTFRVKNKRNVHDGSTELLLSPKRLSSFVSPSSCSSSNCLVITIVDAQPEAPLQPQSSASPIVEEYRSSSFDSIASVSRRWTVWNCASMLCQTSARILVWMLGLLYFSSLPLGVYLLIAQRTTVGVILVVACYRHLAASVGGSTDSSNLRDELRRTREKAQELAVKNRSKLTASLRDKSLPKEERAEMERLWVVFSSCLELFHSDMCKVFEMGQTFSLSSKSDPFVQTGMSGGTSDVAARALSLQTLTHDENLPSIDRLEQNDLEAEIAKVDKMIDNMEMKVNVLRWTVEAKSPLYADPLSTETSSVALLSLDEEDGGRCCDRSQFFVSVVLCGIAMVAVVLSVCVVYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0L6</accession>
    <name>W5N0L6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein insertase that mediates insertion of transmembrane proteins into the mitochondrial outer membrane. Catalyzes insertion of proteins with alpha-helical transmembrane regions, such as signal-anchored, tail-anchored and multi-pass membrane proteins. Does not mediate insertion of beta-barrel transmembrane proteins. May play a role in apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PSEN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0L6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014204.1">
        <property type="protein sequence ID" value="ENSLOCP00000014175.1"/>
        <property type="gene ID" value="ENSLOCG00000011538.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000020"/>
    <dbReference type="HOGENOM" id="CLU_058300_2_0_1"/>
    <dbReference type="InParanoid" id="W5N0L6"/>
    <dbReference type="OMA" id="TSHEMVM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011538">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000022">
        <property type="entry name" value="mitochondrial carrier homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780:SF3">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="32"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="146"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="345" mass="37774" checksum="051F0C309EDCE226" modified="2014-04-16" version="1">MVTPENLTGAGGVRRDPRPDRGGSAAGIGGTPAEIDTAIVLLGAGVTAITHPLLYVKLLIQVGHEPQPPKLGTNLFGRKVLYLPGFFSYARHIVQVDGKRGLFRGLCPRIVSSAISTVVRSKVKQVIPIEDMEHVSNKDDIKTSLRKVVKKTSHEMVVQCLSRIATHPFHVISVRCMAQFVGREVKYSGLFSSIMKIFQEEGVSGFFVGLVPHVLGEVIFLWCCNLLAHFINTYAVDDNVSSRLCPLGVARCPISVQFSQAPAVRSYTKFVMGIAVSVLTYPFLLVADLMAVNNCGLATGLPPNSPIFKSWAHCWRHLSVQGQLFRGSSFFFRRVPLEMAVLSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2R1</accession>
    <name>W5N2R1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="9">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01000316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635184.2">
        <property type="nucleotide sequence ID" value="XM_006635121.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2R1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014920"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014949.1">
        <property type="protein sequence ID" value="ENSLOCP00000014920.1"/>
        <property type="gene ID" value="ENSLOCG00000012127.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683325"/>
    <dbReference type="KEGG" id="loc:102683325"/>
    <dbReference type="CTD" id="403052"/>
    <dbReference type="eggNOG" id="KOG1006">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154744"/>
    <dbReference type="InParanoid" id="W5N2R1"/>
    <dbReference type="OMA" id="HILLEFC"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012127">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06616">
        <property type="entry name" value="PKc_MKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000090">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000126">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF15">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="131"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="428" mass="48270" checksum="5A973289FA01E6A3" modified="2014-04-16" version="1">MATPSPSTNSTTSSSNNSNNAGSTSTSHQHQSQSQHITTVSSMQETNTCWRCQNETGFQINLSGVPQSKRKALKLNFANPPIKSTARFTLNTAGAPFQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRNGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEERQFSPKFINFVNLCLTKDESKRPKYKELLKHPFILMYEERTVDVASYVCKILDQMPASPSSPMYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N369</accession>
    <name>W5N369_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Interleukin family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Immune regulatory cytokine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with IL10RA and IL10RB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the IL-10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628630.1">
        <property type="nucleotide sequence ID" value="XM_006628567.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N369"/>
    <dbReference type="FunCoup" id="W5N369">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015078"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015107.1">
        <property type="protein sequence ID" value="ENSLOCP00000015078.1"/>
        <property type="gene ID" value="ENSLOCG00000012252.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692739"/>
    <dbReference type="KEGG" id="loc:102692739"/>
    <dbReference type="CTD" id="3586"/>
    <dbReference type="eggNOG" id="ENOG502S22U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183124"/>
    <dbReference type="HOGENOM" id="CLU_127747_0_0_1"/>
    <dbReference type="InParanoid" id="W5N369"/>
    <dbReference type="OMA" id="PFDINNF"/>
    <dbReference type="OrthoDB" id="9931894at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012252">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140105">
        <property type="term" value="P:interleukin-10-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905298">
        <property type="term" value="P:regulation of intestinal epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
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        <property type="term" value="P:response to lipopolysaccharide"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="4_helix_cytokine-like_core"/>
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    <dbReference type="InterPro" id="IPR000098">
        <property type="entry name" value="IL-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020443">
        <property type="entry name" value="IL-10/19/20/24/26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482:SF5">
        <property type="entry name" value="INTERLEUKIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482">
        <property type="entry name" value="INTERLEUKIN-19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01294">
        <property type="entry name" value="INTRLEUKIN10"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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    <feature type="chain" id="PRO_5031609516" description="Interleukin family protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="181"/>
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    </feature>
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        <location>
            <begin position="33"/>
            <end position="130"/>
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    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="83"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008813"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR620443-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="21251" checksum="CA5F5F612F9297D5" modified="2014-04-16" version="1">MAFSWLSTLLFLLSALLVENTQIKKNVCWDNCCLFIENFPVRLKELRTAFSQIRDYYEASDELIDTALLDEALLEELHSPFGCHAMKEVLRFYLDLVLPAAVKDKGNDFKHPLDSIGNIFIDLKANLNRCRRYFSCKKTFEIENITNEYRKMEGKGLYKAMGELDLLFNYIEEYLVRKRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3Z8</accession>
    <name>W5N3Z8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01032703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000015386.1">
        <property type="protein sequence ID" value="ENSLOCP00000015357.1"/>
        <property type="gene ID" value="ENSLOCG00000012476.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1077525_0_0_1"/>
    <dbReference type="InParanoid" id="W5N3Z8"/>
    <dbReference type="OMA" id="AWHATAS"/>
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        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012476">
        <property type="expression patterns" value="Expressed in zone of skin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="258" mass="25452" checksum="6F9B243C6606B884" modified="2014-04-16" version="1">MGMFVPEGPGGYITIGAPGIPRVAVPPPCPPFAPTAPSPPAAPVPPFPPSVASVPPYPPPTASVPPFPPPAASVHPYPPPAAPVPPYPPPAAPVPPYPPPAAPVPPYPPCAAPVPPYPPXAAPVPPYPPPAAPVPPYPSPAAPDPPYPAPAVPYPPYPAPAVPYPPPAAPVSPYPPPAAPVPPYPPLSVPTPPNPALKIGARPPPPPKPALPPPLPAPAAPNPPVPSPPPPSTTTPPPPPDSPPAPAGGQGLITYING</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4M2</accession>
    <name>W5N4M2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sphingomyelin synthase 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphing-4-enine 1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:36079"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:73203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:36080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630076.2">
        <property type="nucleotide sequence ID" value="XM_006630013.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047506.1">
        <property type="nucleotide sequence ID" value="XM_069191405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4M2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015581"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015610.1">
        <property type="protein sequence ID" value="ENSLOCP00000015581.1"/>
        <property type="gene ID" value="ENSLOCG00000012660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690873"/>
    <dbReference type="KEGG" id="loc:102690873"/>
    <dbReference type="CTD" id="556193"/>
    <dbReference type="eggNOG" id="KOG3058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157370"/>
    <dbReference type="HOGENOM" id="CLU_027104_0_1_1"/>
    <dbReference type="InParanoid" id="W5N4M2"/>
    <dbReference type="OMA" id="SKFPLEW"/>
    <dbReference type="OrthoDB" id="422827at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012660">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006686">
        <property type="term" value="P:sphingomyelin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF24">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="12">
        <location>
            <begin position="229"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="368" mass="42543" checksum="F4D8D76BC251BA3E" modified="2014-04-16" version="1">MASPQLLREGDSVNTHLDLQVEEEHTELSNGCPRTNLMSASCDGSSSGHHKQSALPNSLRKGIRKRQDYIKISVPESKNRLPLEWWKTGIAFVYACFNLVLTTVMITVVHERVPDKDNSPPLPDKFFDYVDRVQWAFSVTEINGMVLVGLWFVQWLFLRYKAIAGRRFFFLVGTLYLYRCITMYITTLPVPGMHMNCAPKLYADSQAKIQRILRLISGGGLSITGSHMMCGDFLYSGHTVMLTLTYLFIKEYSPRRFWWYHLVCWLLSAVGVVCILVAHEHYTVDVVVAYFITSRLFWWYHTMANVQALKTCSNNYLSRVWWNPVFRFFEKNVQTQVPCTFAWPISWPPSCFKNSCKNYSMVQSSRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4W0</accession>
    <name>W5N4W0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA methyltransferase 10 homolog B</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA (guanine-9-)-methyltransferase domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA (guanine(9)-N(1))-methyltransferase TRMT10B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01001877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200874.1">
        <property type="nucleotide sequence ID" value="XM_015345388.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047630.1">
        <property type="nucleotide sequence ID" value="XM_069191529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4W0"/>
    <dbReference type="FunCoup" id="W5N4W0">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015669"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015699.1">
        <property type="protein sequence ID" value="ENSLOCP00000015669.1"/>
        <property type="gene ID" value="ENSLOCG00000012735.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692074"/>
    <dbReference type="KEGG" id="loc:102692074"/>
    <dbReference type="CTD" id="158234"/>
    <dbReference type="eggNOG" id="KOG2967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063169"/>
    <dbReference type="HOGENOM" id="CLU_034384_8_0_1"/>
    <dbReference type="InParanoid" id="W5N4W0"/>
    <dbReference type="OMA" id="ALQAWFP"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012735">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18100">
        <property type="entry name" value="Trm10euk_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000002">
        <property type="entry name" value="tRNA methyltransferase 10 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047911">
        <property type="entry name" value="Trm10_B_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF19">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="124"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="341" mass="38757" checksum="FF14F390054D62CE" modified="2014-04-16" version="1">MKGTMVERETEKSEHSLSCGMSKGQHKSEDPHICGEESSSTQEMFELLRIDVDSETLKIPRTDSQDSSCSRNVLRKQKNWEKTLAAKKGKRREEKQRRKLKRIEESGNMLQSSQHSKRVVKAITKERLIEAKTVGPRLCIDLSMTDLMSHKEISRLAGQIRRLYGSNRKAANPFHLYLTGLREDGLLYKECLRKNDGFLNYVMDVTEDSWLDLFPLDSVIYLTPDSEHALEHVDPDKVYILGGLVDESIQKKITYQKAMDSCVCTARLPIQEYMVKKTNAKNFHSKILAINQVFDILLTFCETQSWPEALKAGVPPGKGYVLYGESAPDAHLCAESALETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4Y7</accession>
    <name>W5N4Y7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">INO80 complex subunit B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200855.1">
        <property type="nucleotide sequence ID" value="XM_015345369.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4Y7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015696"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015726.1">
        <property type="protein sequence ID" value="ENSLOCP00000015696.1"/>
        <property type="gene ID" value="ENSLOCG00000012755.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688031"/>
    <dbReference type="KEGG" id="loc:102688031"/>
    <dbReference type="CTD" id="83444"/>
    <dbReference type="eggNOG" id="ENOG502QUQX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001754"/>
    <dbReference type="HOGENOM" id="CLU_070409_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4Y7"/>
    <dbReference type="OMA" id="MPCGVIG"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012755">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23021">
        <property type="entry name" value="zf-HIT_IN80B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="3">
        <location>
            <begin position="204"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="329" mass="36855" checksum="6E655B2BB0C34911" modified="2014-04-16" version="1">MGKKKDMIQEKFLSGDGDEGGGHNAHKKKHKKHKKHKKKHHQEEGGHSFSSEALESDSGVVKPQLKLKIKLGGQTLGTKSVPTFTVVPEENRSPSPLMIVDDDEEPTEGVPIEQYRAWLDEDSNLDPSPLPDMDSESLLGGPEDEEEKWLDALEKGELDDNGELKKEIDESLLTARQRALLHKQQSQPLLELPMGYKAKEMTEEMMQKREERARKRRLQAAKKAEENKNQTIERLTKTSKAKIKTMREKKSKQSQVPMIRYTDSFQGTMISFPQGVPTPVVTSPCPTPVAVHCGVTGCSNLKKYSCSKTGTPLCSLECYKKNLLVQPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N592</accession>
    <name>W5N592_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger and BTB domain-containing protein 17-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5R8</accession>
    <name>W5N5R8_LEPOC</name>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        </reaction>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
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    <dbReference type="Ensembl" id="ENSLOCT00000016007.1">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATAXIN-3-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="15">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="300"/>
            <end position="354"/>
        </location>
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        <location>
            <begin position="255"/>
            <end position="264"/>
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    </feature>
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        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
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        <location>
            <begin position="302"/>
            <end position="315"/>
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        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
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        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
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        <location>
            <position position="14"/>
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        <location>
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            <position position="119"/>
        </location>
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        <location>
            <position position="134"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR633865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="354" mass="39724" checksum="FF72FDCA9FC43DEF" modified="2014-04-16" version="1">MDSIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAQQLDEEERIRMAEGGVTSEEYRTFLQQPSGNMDDSGFFSIQVISKALGVWGLELILFNSPEYQRLMIDPINEKAFICNYKEHWFTVRKLGQQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVINGNLPECDADQILGIMRVEQRQRPRLIGEEVAQSGSGQGLVMDRTLNSNQNLEAVPVDEDEENLRKALALSRQDIEVEDEEADLRRAIQLSMQGTTGNTKLPDPKDENIAHTSTSGQNETLTAEELRKRRQAYFDKQSTQQARATAPQQQESKSTGTLANDTGEAPNEQNATQSTLGLTPEQPDSKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6A5</accession>
    <name>W5N6A5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tripartite motif containing 16</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6A5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016164"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016194.1">
        <property type="protein sequence ID" value="ENSLOCP00000016164.1"/>
        <property type="gene ID" value="ENSLOCG00000013122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695079"/>
    <dbReference type="KEGG" id="loc:102695079"/>
    <dbReference type="CTD" id="10626"/>
    <dbReference type="eggNOG" id="ENOG502QRVY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161116"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_2_1"/>
    <dbReference type="InParanoid" id="W5N6A5"/>
    <dbReference type="OMA" id="SAWHSGV"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013122">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12890">
        <property type="entry name" value="SPRY_PRY_TRIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF10">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="178"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="410"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="53"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="601"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="226"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="604"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="627" mass="70578" checksum="BFBBA7D9ACDAC06C" modified="2014-04-16" version="1">MPDTETAKPPDRPACPICSGGFEDPVTFKCGHCFCRGCLGETWDKAESKDVTSCPQCQDTSQFRSPPGENGLMNGTGEHQAETAEESNGEKTPEEEMQEPGEGEGKEKAEEVERVEEEETLAALGPDDVVCDSCIETHSRAVKSCLTCMVSYCESHLRPHLENVKFQNHRLVEPLRDIESRTCETHRQSLDVFCLADNSCLCQGCMEEGHRGHNTVPVGDARKQIEEEMQKKCAEMIKTLTAAENAISKLETNTKSIENSVKDVKATIEEQFSALVEAVEKAKKEVKEFLVNEKSSAVKQAEGIKAHLEQKCTELKKTQVQVEKLSKKKNDIDFLQDYCEWKQGAPDVTLPGVYIGLMDRLPSFSRVVIESTKEICGQLLSTYRDNLKEVCKNDKCSIKTTVHAIIPLKHHISMPEPQTRSDFLKYTSPMTFDPDTAHRFLRLTEDNRKVTNTTPWQHPYMDVPERFEHWRQVLASESFYLGRHYFEADISGEGTHVGVTYKSIDRKGQENNSCITGNDFSWCLQWNAKQFSAWHSDVETPLKVEKFTRIGVYVDYSRGVLGFYGVADTMTLIHKYKAKFQEPLYPAFWLSKKENTVQLVGPGDAPPQKTPSAPPTLPTIPTLSAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7V1</accession>
    <name>W5N7V1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DNAAF4. Interacts with DLEC1. Interacts with SPMAP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7V1"/>
    <dbReference type="FunCoup" id="W5N7V1">
        <property type="interactions" value="1942"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016710"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016740.1">
        <property type="protein sequence ID" value="ENSLOCP00000016710.1"/>
        <property type="gene ID" value="ENSLOCG00000013549.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074988"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_2_1"/>
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        <property type="component" value="Linkage group LG9"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
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        <property type="term" value="F:unfolded protein binding"/>
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        <property type="term" value="P:protein folding"/>
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        <property type="term" value="P:retina layer formation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:skeletal muscle thin filament assembly"/>
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        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
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        <property type="entry name" value="T-complex protein 1 subunitTheta, putative"/>
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        <property type="entry name" value="GroEL"/>
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        <property type="entry name" value="GroEL-like equatorial domain"/>
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        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
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        <property type="entry name" value="Chap_CCT_epsi"/>
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        <property type="entry name" value="Chaperone_TCP-1"/>
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    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
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    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="541" mass="59266" checksum="422E69CFE82ADAA7" modified="2014-04-16" version="1">MSTLGTLAFDEYGRPFIIIKDQDKKSRMSGLDALKSHIMAAKAVANTLKTSLGPNGLDKMMVDKDGEVTVTNDGATILSLMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEQAEQLLDRGIHPIRIADGYDQAARVAIEQLDRIGDSFPVDPGNTEPLVQTAMTTLGSKVINRCHRQMAEIAVNAILTVADMDRKDVDFELIKVEGKVGGKLEDTQLIKGVIVDKEFSHPQMPKVLKDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIHQVKDNGANLAICQWGFDDEANHLLLQNQLPAIRWVGGPEIELIAIATGGRIVPRFCELTPEKLGSAGIVRELSFGTTKDRMLVIEECKNSRAVTIFIRGGNKMIIEEAKRALHDAVCVIRNLVKDNRIVYGGGASEIACALAVNEAADKCPSLEQYAMRAFADALEVIPMALAENSGLNPIQTMTEVRARQVKENNPALGIDCLNQGTNDMKQQHVIETLIGKKQQISLATQVVKMILKIDDIRSPGESED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8E8</accession>
    <name>W5N8E8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L23</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638399.1">
        <property type="nucleotide sequence ID" value="XM_006638336.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8E8"/>
    <dbReference type="SMR" id="W5N8E8"/>
    <dbReference type="FunCoup" id="W5N8E8">
        <property type="interactions" value="1313"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016907"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016937.1">
        <property type="protein sequence ID" value="ENSLOCP00000016907.1"/>
        <property type="gene ID" value="ENSLOCG00000013711.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687377"/>
    <dbReference type="KEGG" id="loc:102687377"/>
    <dbReference type="CTD" id="9349"/>
    <dbReference type="eggNOG" id="KOG0901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004690"/>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_1"/>
    <dbReference type="InParanoid" id="W5N8E8"/>
    <dbReference type="OMA" id="MIQMQTR"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013711">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="140" mass="14865" checksum="807E14139B2EB0B5" modified="2014-04-16" version="1">MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9F5</accession>
    <name>W5N9F5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">UF2 component of NDC80 kinetochore complex</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9F5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017264"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017295.1">
        <property type="protein sequence ID" value="ENSLOCP00000017264.1"/>
        <property type="gene ID" value="ENSLOCG00000014004.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004199"/>
    <dbReference type="HOGENOM" id="CLU_081884_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9F5"/>
    <dbReference type="OMA" id="QVENIDH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014004">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="13">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="141"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="226" mass="26089" checksum="9380D6ED5A295CE9" modified="2014-04-16" version="1">MEAHTFPVYKVDAIVQFLRNNVSDENLTKSDIAPNPKPDTIQRLYMKILHQVFGFRPECHSMMPINENVQYSAILEGVTPVLSVYICMCQFLPMCHIYDFQLNDILSPKVKRTIYILSGIMNFLHFRNLRQGMLAETQQTYKPVMDKLQTLTQEINEAEKKIQELSTIPPEQKAQYEELSAALSDLQNHMDHEVQEVSAVNSQIAELKADHAERSQKLVSTIWLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9J8</accession>
    <name>W5N9J8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fibulin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N9J8">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017307"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017338.1">
        <property type="protein sequence ID" value="ENSLOCP00000017307.1"/>
        <property type="gene ID" value="ENSLOCG00000014039.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692480"/>
    <dbReference type="CTD" id="2199"/>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156047"/>
    <dbReference type="InParanoid" id="W5N9J8"/>
    <dbReference type="OMA" id="MNTCRDI"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014039">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000341">
        <property type="entry name" value="Fibulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000630">
        <property type="entry name" value="Fibulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000078">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000139">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056612">
        <property type="entry name" value="FIBL-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24532">
        <property type="entry name" value="FIBL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00104">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="960"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="1003"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="1045"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="1084"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="618"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="703"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="633"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1286" mass="143059" checksum="557B3996906157D7" modified="2014-04-16" version="1">MLQSLERQKQIVEIFTDFFINKVYLTFGAESKMAKYKLRKSILFMTVLVLCISGALCQKDCTGVDCPLLEHCIEEVLERGACCASCLQKGCTCEGYQYYDCINAGFRNGKVPEGESYFVDFGSTECSCPKGGGKISCHFIPCPEVPPHCIEFSEPSDGCLQCERFGCVHDGHKYDAGHTFQMDPCKVCHCPHAGGELMCYPVPDCDLGTFQTPPLFPTAHEENTSEKNNDELSNYERAGSEDRLSQISHLPQSEKLPLFKTDIKDEDEDYDYTPTDSAKSSLFDLAVPTKSAVTLVSYSDKPTAHEMFNEDLKQELRQKIRTQEEETKNEEITEDPLVGPTSQVFQDVRKSMEGISGKGAEDYVTPHMAEEEHEEDEQVVTLKDVTEGGTDRKEVTYVIDHELQTTETVPAVESNSHSSTTEENFLVQEDPLGKHGQAVLPTIKFSPTTQPPQIMKAVEALISKKQSQTLYNYQPERERRNNSVDSHPASQGVSGHLLVASCCEAGQKWASDNGHCTGMPQREVNTCRIAEQQCCLGHLRDSKCLAGMTAAKEGKMCELGERDICGADSYMECCSCCSLGLQFRSEGHNCDSHQHLVYPCSHMFLTCCEGEDGLSQPTLRERQKPEPTSLPKRVSDGTYDKEAFSITDEEGNENTVEKGEDIDECLEYEGQLCHQRCINTRTSYECACFPGYILLSDGYSCEPENPEEEDNRLREEDRPATPVPTVAASTTVIPVDLCAGNGPCMHVCSQAAGEVLCSCFPGYALMADGHSCEDVNECHSGTHSCSRGVLCVNSKGSFQCVRVNEPCEEGFIYNVNRECVDINECLTNTHSCQAMERCINTIGSFTCEKQITCSPGYQLKEDTCEDIDECLQQTHNCGTGFECQNTAGSFYCNPKHRCFTGFSQDQHGNCIDIDECSSPNVPCSPGFNCINTVGSYTCQRKIIVCSRGYHASPDGARCIDVDECQTGVHRCGEGQICHNLPGTYRCDCKTGYQYDMFRKVCVDVNECWRYPGRLCAQTCENTPGSYQCSCTTGFTLSSDGKNCEDVNECEKNPCSQECANIYGSYQCYCRQGFYLKEDGHSCEDIDECSQSVGNLCAFQCVNVPGSYQCACPAHGYTMSPNGRTCRDIDECAVGTHNCSTAETCYNIQGGFRCLSFSCPPNYRRVSDTRCERLSCPNYVECQSSPLRITYYHLSFQTNIVIPAQIFRIGPSPAYSGDNIIISITRGNEENYFSARKLNSYTGAVYLQRQVREPKDFLIDVEMKLWRQGNFTTFLARIYVFITAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA36</accession>
    <name>W5NA36_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cadherin-related family member 4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NA36"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017495"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017526.1">
        <property type="protein sequence ID" value="ENSLOCP00000017495.1"/>
        <property type="gene ID" value="ENSLOCG00000014204.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107077432"/>
    <dbReference type="KEGG" id="loc:107077432"/>
    <dbReference type="eggNOG" id="KOG4289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162824"/>
    <dbReference type="HOGENOM" id="CLU_382923_0_0_1"/>
    <dbReference type="InParanoid" id="W5NA36"/>
    <dbReference type="OMA" id="LVHNDLM"/>
    <dbReference type="OrthoDB" id="9949162at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014204">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000260">
        <property type="entry name" value="Cadherin related family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000291">
        <property type="entry name" value="Cadherin related family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000300">
        <property type="entry name" value="Cadherin related family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF441">
        <property type="entry name" value="CADHERIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867462" evidence="8">
        <location>
            <begin position="35"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="242"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="345"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="459"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="9">
        <location>
            <begin position="590"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="756"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
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            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="817" mass="89099" checksum="C5325CEF3D442DBE" modified="2014-04-16" version="1">MCLFLELNMRGTMNTVFTFSLSLILLMQVHESHSQTLSFTTETVSIPETSPPGTVVFVFTFSPASSIPTINCVGSSSCSSIFQLRATGTTATITLSSSAVLNHKITNQYTKDIYISYIASGTTFSAQARLYVVITPAPKCDKLFASAGGATVQVLQNLAPSSIIYIVNPADPNPGSYKYSMTPEKPFGININTGSVTSPEKGFSGPGSFKLSITVNGMNGTSCSGILHVKVLPVETANVTFPKSSYAVSIDENTGPGASVMTVLAKGSDIYYQIVPQTSGGYAAYQIHQRTGEIRTTYNLDLEANPHLNSTVFLVQAYDPIHKVSATATVSITVRDVNEFPPVCHPALIVAQIKESEPIGYKFPSDDPVKCVDMDRMDSSLTYSIVTNENSKDKFRISPQGGYLVLNSTLDYDSYEVANVNFLYTASIIVTDNGKPPLTTTIPVYITVTQVNEHPPVFSPCPQAMQVNENAPLGTVIGQVNATDRDWPFNNVEYSIAGGAGNNPPKFYIDPRSGEIHVLSHLDYEKQTIYQLKIKAVDMNQDISPDPQMQKTAFCTVTVRVQDENDNPPVCKPPYYEELIYSTLKTDVGITTIQCTDIDTPIEDLTYSIVRGNVNNRFTMKGSALCSRNAFSFKTPGVYDPTDYELLIEVKDKGPPVFSTTATVIVHVVPWTTTQPTTTVKTTHRPRPTKIVTLTVFEWVPDTWFVVVLTVAGALLLLALALLTWKLLTLTSVCGRAPIEATKPLLQDSGGQFRGVFDDAGPTSPSNLHQQSSTPPAADKEIPESPLSLQRFDGRAQDPITGREYIFNSVTGQRRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBI8</accession>
    <name>W5NBI8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01023753">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NBI8">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017997"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018029.1">
        <property type="protein sequence ID" value="ENSLOCP00000017997.1"/>
        <property type="gene ID" value="ENSLOCG00000014624.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160518"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_14_1"/>
    <dbReference type="InParanoid" id="W5NBI8"/>
    <dbReference type="OMA" id="CNQRKKS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014624">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000079">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF39">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="SH3-domain"/>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="103"/>
            <end position="371"/>
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        <location>
            <begin position="458"/>
            <end position="483"/>
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            <begin position="529"/>
            <end position="542"/>
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            <begin position="584"/>
            <end position="593"/>
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        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017997.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="652" mass="71952" checksum="35907A4D1752A840" modified="2014-04-16" version="1">PGRPGFPERDWIAPPTPPALWTAVFDYEAAAEEELTLRRGDVLEVLSKDSTVSGDEGWWTGQIQDKVGVFPSNYVTCGPHYSQLQGSLGQERARPLEVDFSELQLEEVIGAGGFGKVYKGLWRGEEVAVKAARQDPDEDISVTAESVRQEARLFWMLRHPNIIALKGVCPKEPNLCLVMEYARGGALNRALAGRKVAPRVLVNWAVQIATGMNYLHDQAIVPIIHRDLKSSNILILERVEHDDLSCKTLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIKLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFAKLLEECWNPSPHSRPPFGTILAQLQAIEQSAMFQMPLESFHSLQEDWKLEIQQMFXREQELAEREIDIVERELNIIIHQMYQEKPKVKKRKGHFKKSRLRLGKDGNRISLPSGFEHKITVQASPSVDKRKGQGGSDGTTPPGSPVVIPRLRAIRLTPSDGSKTWGRSAVCKKEELAANKKKGRTWGPSSTHQRERLGGEDRLKSLGEGCKQWSSSAPNLGKSPKHMPMTGGFASLNEMEEHCEPQSPLSRLSLPPGEAGSNGALDDSGAPPCSAGCGTPTDSARRCAQRKRSDLLLLGCASLLASIGLGQDLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBS4</accession>
    <name>W5NBS4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytoskeleton associated protein 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <dbReference type="STRING" id="7918.ENSLOCP00000018083"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018115.1">
        <property type="protein sequence ID" value="ENSLOCP00000018083.1"/>
        <property type="gene ID" value="ENSLOCG00000014683.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014757"/>
    <dbReference type="InParanoid" id="W5NBS4"/>
    <dbReference type="OMA" id="NWKERKE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014683">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051298">
        <property type="term" value="P:centrosome duplication"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
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    </dbReference>
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        <property type="term" value="P:mitotic spindle organization"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="16">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="16">
        <location>
            <begin position="268"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="14">
        <location>
            <begin position="440"/>
            <end position="478"/>
        </location>
    </feature>
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        <location>
            <begin position="575"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="16">
        <location>
            <begin position="837"/>
            <end position="1069"/>
        </location>
    </feature>
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        <location>
            <begin position="1222"/>
            <end position="1460"/>
        </location>
    </feature>
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        <location>
            <begin position="501"/>
            <end position="564"/>
        </location>
    </feature>
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        <location>
            <begin position="800"/>
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        </location>
    </feature>
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            <begin position="1069"/>
            <end position="1158"/>
        </location>
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        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
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        <location>
            <begin position="1451"/>
            <end position="1512"/>
        </location>
    </feature>
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        <location>
            <begin position="1921"/>
            <end position="1956"/>
        </location>
    </feature>
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        <location>
            <begin position="1976"/>
            <end position="2006"/>
        </location>
    </feature>
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        <location>
            <begin position="2019"/>
            <end position="2063"/>
        </location>
    </feature>
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        <location>
            <begin position="519"/>
            <end position="560"/>
        </location>
    </feature>
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            <begin position="819"/>
            <end position="832"/>
        </location>
    </feature>
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            <begin position="1069"/>
            <end position="1084"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
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            <begin position="1493"/>
            <end position="1509"/>
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    </feature>
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    </feature>
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    </feature>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2063" mass="229263" checksum="1EFD18742D7BA70E" modified="2014-04-16" version="1">MGDDSEWMKLPIDQKCEHKLWKARLNGYEEAVKLFQRIEDEKSPEWSKYLGLVKKFVTDSNAVAQLKGLEAALAYIENAHVAGRTTGEVVSGVVSKVFNQPKARAKELGTEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACVETLRKALSEFGSKIVTLKPIIKILPKLFESREKAVRDEAKMFAVEIYKWIRDALRPPLQNINSVQLKDLEEEWVKVPPSAPKQSRFLRSQQDLKVKFEQQQAAGADEVDGDDEESAPQVDPYELLEAVEILSKLPKDFYEKIVKSLKVLEQKEALEALEVLTKNPKIENGDFGDVVRALKKVIGKDTNVMLVALAAKCLAALASGLRKKFGTYAGHVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNVSEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKGLLKPFCAALLKQINDSAPEVRDAAFEALGTAMKVVGEKAVNPFLTDVDKLKLDKIKECAEKVELAGGKKSAGGTEKKEKPVAKPEPAAEAPAKSAAPSKKAPISKPKGTGPPKKGKPSAAAAAGVKGKKATETKEVVEAELSIEVCEEKAAAVLPASCMQLLDSGNWKERLASMEEFQRAVEQMDRNEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVGLVAQKGVFSKTSAFVVLDGLVDKIGDVKCGAKSKEALTAIGEACSLPWTAEQVVSLAFAQKNPKNQAETLNWLANAMKEFGFGGINVKAFINNVKTALGATNPAVRTAAISLLGVMYLYMGAPLRMFFEDEKPALLAQIDAEFEKMQGQSPPAPFRGTKKGGEEEVEEKEEQEEEGGNDIMDLLPRSDICDKITSELVSKIGDKNWKIRKEGLDELTAVLNEAKFIQPNIGELPIALKGRLNDSNKILVQQTLTILQQMATALGPGLKQHVKSLGISIITVLGDSKSNVRAAALTTLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQEVLGWLAEKLPTLRTVSPDLMFCVPYLYACLEDRNGDVRKKAQDALPTFMMHLGYEKMLKATGKLKTASKDQVVGMLEKARAIMPAKPAAPAKAGPSKSGGTAPAAKQAAGPTKPQSVSDDSGIGIADSKPDSKKTKPAAVKGKKASQDVNDSMEKDNSLNKPNRQSKGKPSKQVQISVSISFQAAAGKKAPVVKPNVKEEDDKSGPMFILVPNGKEQRIKEEKGLKILKWNFITPRDEYVEQLKTQMATCLAKWLQDELYHFDFQHHIKALSAMIEHMEEEKDATIGCLDLILKWFTLRFFDTNTSVLMKALEYLKLLFAMLSRENYHLTEYEASSFVPYLILKVGESKDVVRKDVRAILNMLCKVYPASKVFTFLMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPAKALKEIAVHIGDRDTSVRNAALNTVVAVYNVCGEQVFKLIGNLSEKEMSMLEERIKRSAKKAPPPVKQVEEKPQRPQPGNPNASLMRKPPQDEVPSKLNQARTQNAHPEQSAPSVPKEFQLDLDVIENDHTRVSELPDLVQHKLDELLEPVMIPEPKVRAVSPHFDDLHSSTASTINFVISQVASGDINTSIQALAQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADERQDKEDIVKLYSCIIGNMLSLFYMESLAREASMGVLKDLMHGLITLMLDTRVEDLEDGQQLIRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMIRLLPETINNINLDRILLDVHNFMKIFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGSKILDHLTMIENKNESELEVHLRRVVKHSIDPSNTKSEKDTEKGAIRADEKMSKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADIEPFLKNTSQFFQSYVERGLRMIESEREGKGRIQTSNTGIPQHSTETTTYVPTSSSVPANTNGEEVKPAVYLERLKILRQRHGLENNSKQQEERPPLTSLLSKPTVPTVASSTDMLHSKLSQLKESREHYHQELESNQSHVQSGMGSTTTAANLDDLKKRLERIKSNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBV4</accession>
    <name>W5NBV4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ectodysplasin-A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Homotrimer. The homotrimers may then dimerize and form higher-order oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBV4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018113"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018145.1">
        <property type="protein sequence ID" value="ENSLOCP00000018113.1"/>
        <property type="gene ID" value="ENSLOCG00000014715.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682171"/>
    <dbReference type="KEGG" id="loc:102682171"/>
    <dbReference type="CTD" id="1896"/>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111220"/>
    <dbReference type="HOGENOM" id="CLU_062448_0_0_1"/>
    <dbReference type="InParanoid" id="W5NBV4"/>
    <dbReference type="OMA" id="PRGAPCM"/>
    <dbReference type="OrthoDB" id="6159739at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014715">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042476">
        <property type="term" value="P:odontogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042475">
        <property type="term" value="P:odontogenesis of dentin-containing tooth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000004">
        <property type="entry name" value="Ectodysplasin-A isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051748">
        <property type="entry name" value="TNF_Ligand_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151:SF13">
        <property type="entry name" value="ECTODYSPLASIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151">
        <property type="entry name" value="PROTEIN EIGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-0176">Collagen</keyword>
    <keyword evidence="6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="21">
        <location>
            <begin position="227"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="110"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="372" mass="40532" checksum="D13297D3F7B13E4E" modified="2014-04-16" version="1">MASKGLTENVANLEEKALKPELPTTCMCRKQCNNCKIFLGFFVLSLSLHLITLFCYLDLRSEVKRELSQKNRDEIVTPGATDLNEPLSPEYHLLDISYPTMDQEVASNVFISEDSSHSTEENKRVIHRVKRSPSKRPETDPESKQRRKGKKGPPGAPGPPGPPGPPGPQGPPGIPGIPGIPGSNAMGPAGPPGPPGPAGPPGPQGPPGPQGPAGGTDKTRARDMQPAVVHLQGQETTIQVKEDLSEGVLKNWKMISIHPKVFKMHSRSGELEVLVDGTYFIYSQVEVYYLNFTDIASYEVMVDKTPFLRCTRSIETGQRKFNTCYTAGVCLLKARQKISIKMVYEDTSISMTNHTTFLGSIRLGEAPSSGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBZ6</accession>
    <name>W5NBZ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01023843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBZ6"/>
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        <property type="protein sequence ID" value="ENSLOCP00000018155.1"/>
        <property type="gene ID" value="ENSLOCG00000014743.1"/>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:creatine kinase activity"/>
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        <property type="term" value="P:phosphocreatine biosynthetic process"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Creatine kinase M-type"/>
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        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
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        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
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        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
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        <property type="entry name" value="CREATINE KINASE"/>
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        <property type="entry name" value="ATP-gua_Ptrans"/>
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        <property type="entry name" value="ATP-gua_PtransN"/>
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        <property type="entry name" value="Guanido kinase N-terminal domain"/>
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        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
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    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0418">Kinase</keyword>
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    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="11">
        <location>
            <begin position="125"/>
            <end position="367"/>
        </location>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="292"/>
            <end position="296"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="320"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
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            <dbReference type="ARBA" id="ARBA00012231"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
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            <dbReference type="PROSITE" id="PS51509"/>
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            <dbReference type="PROSITE" id="PS51510"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018155.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="377" mass="42713" checksum="396C238514773870" modified="2014-04-16" version="1">MPFGNTHNNYKLNFSVDEEFPDLTKHNNHMAKALTKDIYAKLRDKQTPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYDVFKDLFDPVIEDRHNGFKPTDKHKTDLNFGNLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAIEKMSIDALNTLEGEFKGKYYPLKDMTDEEQDQLIRDHFLFDKPVSPLLLASGMARDWPDARGIWHNNDKTFLVWVNEEDHLRVISMQKGGNMKEVFRRFCVGLQKIEDLFKKHGRSFMWSEHLGYILTCPSNLGTGLRGGVHVKLPQLSKHPKFEEILTRLRLQKRGTGGVDTAAEGGVFDISNADRLGFSEVEQVQMVVDGVKLMIEMEKKLEKGAAIDDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFN8</accession>
    <name>W5NFN8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lin-9 DREAM MuvB core complex component</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFN8"/>
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        <property type="interactions" value="1356"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000019447.1"/>
        <property type="gene ID" value="ENSLOCG00000015793.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694656"/>
    <dbReference type="KEGG" id="loc:102694656"/>
    <dbReference type="CTD" id="286826"/>
    <dbReference type="eggNOG" id="KOG1019">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003188"/>
    <dbReference type="HOGENOM" id="CLU_013763_0_0_1"/>
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    <dbReference type="OMA" id="KEEMIPP"/>
    <dbReference type="OrthoDB" id="2339771at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015793">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="term" value="P:DNA-templated transcription"/>
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    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="LIN9_C"/>
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        <property type="entry name" value="LIN-9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN LIN-9 HOMOLOG"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="560" mass="63785" checksum="14BF1298F8C5B535" modified="2014-04-16" version="1">MHSDRQPLKKRRGSFKMAELEQLLDESSSAKALVSLKEGSLSNTWNEKHSSVPKPQNSKGRSNYSSMETPTRSSKRSRLLCEDEDRQMNSRSPRRSQRVITAPQKLTTVATPDKKASQKIGLRLRNLLKLPKAHKWCIYEWFYSNIDRPLFEGDNDFCVCLKESFPNLKTRKLTRVEWATIRRLMGKPRRCSAAFFAEERSALKQKRQKIRLLQQRKFTDVSHCKDLPEEIPLPLVIGTKVTARLRGVHDGLFTGQIDAVDTSAATYRVTFDRNGLGTHTVPDYEVLSNEPHETMPISAFAQKQRPSRFQAFMTPPRLPYTTSVQHPIMDNDPLLGQSSWKSKLSGTDGETLGGFPVKFLVQVTRLSRILMIKKEHIKLLKEMNTEAEKLKSYSMPIGFEFQKRYATIVLDLEQLNKDLNKVLHEVQAYCYELAPEQGMQPADQPSDLRRRCEEEAQDIVRQANTLSNGHYSIQNESLTDLISRLTALLLQIKCLAEGSDLNSFEFKSLTDSLNDIKNSIDSSNISCFQDNVEIHVAHIQSGLSQMGNLHAFAANNTNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFX3</accession>
    <name>W5NFX3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leiomodin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cardiac leiomodin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Mediates nucleation of actin filaments and thereby promotes actin polymerization. Plays a role in the regulation of actin filament length. Required for normal sarcomere organization in the heart, and for normal heart function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Myofibril</location>
            <location evidence="7">Sarcomere</location>
            <location evidence="7">M line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <dbReference type="Proteomes" id="UP000018468"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFY1</accession>
    <name>W5NFY1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex I subunit B13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Complex I-13kD-B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase 13 kDa-B subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633454.1">
        <property type="nucleotide sequence ID" value="XM_006633391.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015352507.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_069192480.1"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000015870.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="loc:102691344"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008099"/>
    <dbReference type="HOGENOM" id="CLU_099943_2_0_1"/>
    <dbReference type="InParanoid" id="W5NFY1"/>
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    <dbReference type="OrthoDB" id="286811at2759"/>
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        <property type="component" value="Linkage group LG8"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="NDUFA5"/>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
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        <property type="entry name" value="ETC_C1_NDUFA5"/>
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    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
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    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="116" mass="13475" checksum="2AD0F7F16006C1D9" modified="2014-04-16" version="1">MAGLLKKTTGLLGLAVSQNPHERLRILYTKILASLQTIPQDAAYRKYTEQFITERLNLVTSEPDVEKLEKKINSGQIEEVILQAENELHLSRKMLEWKPWEPLVEEPPANQWKWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG78</accession>
    <name>W5NG78_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Presenilin</fullName>
            <ecNumber evidence="10">3.4.23.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The PAL motif is required for normal active site conformation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase A22A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01001616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG78"/>
    <dbReference type="FunCoup" id="W5NG78">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019637"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019669.1">
        <property type="protein sequence ID" value="ENSLOCP00000019637.1"/>
        <property type="gene ID" value="ENSLOCG00000015948.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157923"/>
    <dbReference type="HOGENOM" id="CLU_022975_3_1_1"/>
    <dbReference type="InParanoid" id="W5NG78"/>
    <dbReference type="OMA" id="NTNENCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015948">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034205">
        <property type="term" value="P:amyloid-beta formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019896">
        <property type="term" value="P:axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.100:FF:000001">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.100">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001108">
        <property type="entry name" value="Peptidase_A22A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042524">
        <property type="entry name" value="Presenilin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202">
        <property type="entry name" value="PRESENILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202:SF24">
        <property type="entry name" value="PRESENILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01080">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01072">
        <property type="entry name" value="PRESENILIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="306"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="447" mass="49989" checksum="8A86EA6502166659" modified="2014-04-16" version="1">KIPLMNTSDSEEEFCNERTALVPAESPTVPSYASDVGLPISSDTEAIRRRPAAGSGERQDGRHVDRDSALESEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTEKNGQLIYTPFTEDTSSVGQRLLNSVLNTLIMISVIVIMTIFLVILYKYRCYKFIHGWLILSSLMLLFWFSFMYLGEVFKTYNVAMDYLTLVLVIWNFGAVGMVCIHWKGPLHLQQAYLIMISALMALVFIKYLPEWSAWVILGAISVYDLIAVLCPKGPLRMLVETAQERNEPIFPALIYSSAMMWMVGMAKPSTATQSGNQDSDEETNQNDHHNTNENCEVPPPQVIRDQGRSPPAEDEHEEERGVKLGLGDFIFYSVLVGKAAATASGDWNTTLACFVAILIGLCLTLLLLAIFKKALPALPISITFGLIFYFSTDNLVRPFMDKLAAHQFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGX1</accession>
    <name>W5NGX1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01008883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633564.1">
        <property type="nucleotide sequence ID" value="XM_006633501.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGX1"/>
    <dbReference type="FunCoup" id="W5NGX1">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019880"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019913.1">
        <property type="protein sequence ID" value="ENSLOCP00000019880.1"/>
        <property type="gene ID" value="ENSLOCG00000016134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695205"/>
    <dbReference type="KEGG" id="loc:102695205"/>
    <dbReference type="CTD" id="436767"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161146"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_7_1"/>
    <dbReference type="InParanoid" id="W5NGX1"/>
    <dbReference type="OMA" id="LDYRCQF"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016134">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="204" mass="23071" checksum="BF694358D56A0D52" modified="2014-04-16" version="1">MNDIKLAVLGSEGVGKSALIVRFLTKRFIGEYASSSETVYRKRVSCDGRQMNLEIYDPCSRPCEGQSSLNDQVHWADGFVVVYDISDRSSFTTAKAIIHLIRELQLGVAKRDTDSVVFLVGNKQDLCHMREVDREEGQKLATENKCQFYELSAAEYFQEVAVMFSKVIRNTSVNSKVKDRRRPSGSKSMAKLINNVFGKRRKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI86</accession>
    <name>W5NI86_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein tyrosine phosphatase receptor type O</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01033536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208471.1">
        <property type="nucleotide sequence ID" value="XM_015352985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI86"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020345"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020379.1">
        <property type="protein sequence ID" value="ENSLOCP00000020345.1"/>
        <property type="gene ID" value="ENSLOCG00000016467.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690610"/>
    <dbReference type="CTD" id="5800"/>
    <dbReference type="eggNOG" id="KOG0791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154814"/>
    <dbReference type="InParanoid" id="W5NI86"/>
    <dbReference type="OMA" id="TISFITX"/>
    <dbReference type="OrthoDB" id="8609993at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016467">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072112">
        <property type="term" value="P:podocyte differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003093">
        <property type="term" value="P:regulation of glomerular filtration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000488">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000039">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058859">
        <property type="entry name" value="Fn3_R-PTP-O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042996">
        <property type="entry name" value="PTPRO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47028">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47028:SF1">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26586">
        <property type="entry name" value="Fn3_R-PTP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="455"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="552"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="749"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="962"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="1136"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1240" mass="141100" checksum="6D2AFC6831CDA113" modified="2014-04-16" version="1">MFCQVANRISIFMLPCIICLIFSIQATVSFLVRVRDGNIIVMSLEPTDLTENAVTYVVKVTGETKPYFLEFKNLSDALPAPVVFNASYHGLCYVMTLMMKNTKVELKPVKSVTVLTNPLPVNSVFIHDYQPSPETSVVFEIKCAEKYNVFTRVNISYLEGREQRSMLYKDFFRGKTIFKHWLPGMCYSNITFQLMSEATVNKTTLVQYSGVGHEPKQHRTVPNPPNNVTLQIVQFGRGSMSERAHSRSHTRKVREEVRLLEEEEQDENKEELMRDDEIETSQRPRNLTQNLNDSYSWVDYSYSNGTDYETTTQPYWPHVGTTASSDEDEFVNQVVPEYDDQNELGSAGDLPLPFIPSVRLPPLLLELHWLPPHPPTAFDGFNISIYRDGNSTEMATVDENVHEFFAELTEPGTYVVSVTTFSSSGDCEPRESTSHNSFTFYLSPNGEWFEELKEKPQDINVRILSSSAAEVSWVPSAESHNTTLVTVISLTCHKPNLNQRMESRYCSEENSTSNIITNLTPGAQYKVVVRHVNGPLMGPASDPVVFEIEPTGVRDLVLYPLSPTTVILSWRRPYHVAFRKYVVEMFFFNPVTLTSEWTTYYEIAATHSLTSTVRVTDLLPAWYYNFRVTMVTWGEPELSCCDSSTVSFITAPEAPHISSVDYSNSMLYIRWTYGDVFTDLSHSRMLHWLVVAEGNKGVKKSISSDVTRTVMKAVLSLPPGDIYNLTVTACTERSKNTSAPHIVKVEPAPPKSLYAVNATQTSVTLLWVEEGVVDFYQVYCKQVGSSRELKEKEAQTVNSHVVTISSLLPSTAYNCSVTSYSHNTPSEPTFIAVSTRVTEMNPNVVVISVLAILSIVLISLLLFILIMLRKKHLQMARECGAGTFVNFASFERDGKLPYNWRRSIFALLTLLPSCLWTDYLLAFYFNPWGNTALKKKKLTNPVQLDDFDAYVKDMSKDSAYKFSLQFEDLKSVGLDISHEAADLPVNRAKNRYTNILPYDFSRVKLISMHNDEGSDYINANYIPGYRSPREYIATQGPLPETKNDFWKMVLQQKSHIIVMLTQCNERRRVKCDHYWPFTEEPVTYGEITVEITSEKEATEWTVRNFRLGYADETQDVLHLNYTSWPDHGVPTVNAAESILQFVQIVRHRAARSKGPIIVHCSAGVGRTGTFIALDRLMQHIQEHEYVDILGLVSEMRSHRLSMVQTEEQYIFIHQCVQLMWKKKKQQISAGDIIYENVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIE8</accession>
    <name>W5NIE8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine 5'-monophosphoramidase HINT3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Histidine triad nucleotide-binding protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HINT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626082.1">
        <property type="nucleotide sequence ID" value="XM_006626019.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIE8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020407"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020442.1">
        <property type="protein sequence ID" value="ENSLOCP00000020407.1"/>
        <property type="gene ID" value="ENSLOCG00000016523.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682888"/>
    <dbReference type="KEGG" id="loc:102682888"/>
    <dbReference type="CTD" id="135114"/>
    <dbReference type="eggNOG" id="KOG4359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047616"/>
    <dbReference type="HOGENOM" id="CLU_056776_4_2_1"/>
    <dbReference type="InParanoid" id="W5NIE8"/>
    <dbReference type="OMA" id="EKKCIFC"/>
    <dbReference type="OrthoDB" id="1915375at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016523">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF5">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869794" description="Adenosine 5'-monophosphoramidase HINT3" evidence="10">
        <location>
            <begin position="17"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HIT" evidence="11">
        <location>
            <begin position="44"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="8 9">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="7">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="180" mass="20385" checksum="5040F87B9F0E7B50" modified="2014-04-16" version="1">MTAAIFFVAYGLQVQCLKMAASEGTAIEQSGSLNQTDGYEKKCIFCKIVNKEVDTEVIYFDEELACFKDIKPGAPHHYLVVPMKHIGNCKSLKKDHAPLVEKMVEVGKAMLQKNGITDLNDIRLGFHWPPFCSVTHLHLHVLAPASLMGFTSRIFYRPDSYWFITAEQLLQRLNSFNENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ15</accession>
    <name>W5NJ15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor 1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cyclin-dependent kinase inhibitor p27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">p27Kip1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ15"/>
    <dbReference type="FunCoup" id="W5NJ15">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020624"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020659.1">
        <property type="protein sequence ID" value="ENSLOCP00000020624.1"/>
        <property type="gene ID" value="ENSLOCG00000016694.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159852"/>
    <dbReference type="HOGENOM" id="CLU_077692_2_0_1"/>
    <dbReference type="InParanoid" id="W5NJ15"/>
    <dbReference type="OMA" id="THLRDQK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016694">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050680">
        <property type="term" value="P:negative regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045926">
        <property type="term" value="P:negative regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901990">
        <property type="term" value="P:regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.365.10:FF:000001">
        <property type="entry name" value="Cyclin-dependent kinase inhibitor 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF9">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="17">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="123"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="195" mass="21573" checksum="6787431B69F466F4" modified="2014-04-16" version="1">MSNVRLSNGSPTLERMDARPSDHPKPSACRNLFGLVDHEELRRDLKGHLQEMEEASKATYNFDFANHKPLSPGRYVWEIVEDTEVPRFYTKPPRASKGICPSGNISLDLNGNHSCGGTACHEGTGDRLSGDSAEKPASQTDCRDQRTTPRKRSASHADSSQNKRANTTVDEVALSPNVTRSEEQTPSKSNPRQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK73</accession>
    <name>W5NK73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01034349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034355">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK73"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021032"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021068.1">
        <property type="protein sequence ID" value="ENSLOCP00000021032.1"/>
        <property type="gene ID" value="ENSLOCG00000017014.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159863"/>
    <dbReference type="InParanoid" id="W5NK73"/>
    <dbReference type="OMA" id="XSLVIED"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017014">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bromo_tif1_like"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33"/>
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        <property type="entry name" value="Tripartite motif containing 66"/>
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        <property type="entry name" value="Bromodomain-like"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Bbox_C"/>
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        <property type="entry name" value="Bromodomain-like_sf"/>
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        <property type="entry name" value="Znf_B-box"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
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        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-ALPHA"/>
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        <property type="entry name" value="PHD"/>
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    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="44"/>
            <end position="105"/>
        </location>
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        <location>
            <begin position="132"/>
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        <location>
            <begin position="192"/>
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        <location>
            <begin position="737"/>
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        <location>
            <begin position="828"/>
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            <begin position="927"/>
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            <begin position="302"/>
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            <begin position="489"/>
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        <location>
            <begin position="638"/>
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        </location>
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            <begin position="927"/>
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        </source>
    </evidence>
    <sequence length="955" mass="106945" checksum="1657D7D84FD2024F" modified="2014-04-16" version="1">MDESSEKNNGDKDVVLVAENEAESMQIEEESPKLRSSLNLLDTCAVCTSNFQSREPRLLPCLHSFCKKCLPQPSRHLAVAEKKDPSPPGDHASRSIGVIRCPVCQQECVEIDVMENFFVKDTVEVPSSTVEKTSQVCTSCEDNTEATGFCVECVEFLCLTCIEAHQRVKFTRDHTIRQKEEVSPEAVSISSQRPVFCPIHKKEPLKLFCDTCDRLTCRDCQLLEHKDHRYQFLEDAYKNHKECLETMTMQLQEKKKAVEAVSDVIKSRLQQVDENHKAVKYEINMSIYALFVEIKKRGKALINQLEAMRKDQEAVLNKQQEDINLLSKQLEYVMNFTKWVASNNSSTALLYSKRLILFQIQNLLRAKCEMTFIPQNSVRFQSGLAAWARNIDFGTLMIDSDPAVQQSNFQNAQAALNAAAQKPGASPPGFPSVSSSQPAPAVPPNTLAQLQKQVERLSYRPGRQRQPRGWVWHIHEPPVLKPQGGVQGSRVQSPSVGSQQGPRFGAQGLVPPSPTSNPQNPGFLTHQILVGLVLEIQMPPLPSVQSQNALRNPLPHPSSSSPTAYSAFQQLNRMEAVYLDNRKERNGPITVPKTITSQSLPMSSTDKNGKTEYSGGHYQQRLNFLMPSSATSIHLPRSSSCKSSQSQNKDSFKQKKSTSPYPGIVVKDEPDDDDLSLVSFSTPTILCSAFGSQGQSRVRTNVPDSTEDQAKAVEERLSCWSPSSAGKTGGAEEDPNEDWCAVCQNGGELLCCEKCPKVFHLACHVPPLLAFPRSGEWFCTFCRDLSKPELEYDCDSAVEPTGAKDKESSRRLPPLDKRKCERLLLYLYCHEQSADFQEPVPPSIVPDYYTIIKTPMDLSLVKEKLQAQKQCYSDPEEFVADIRLIFLNCAEFNEPDSEVAAAGKTLQKFFEERLRILYPERKFPEQKVEGVAREGENEPSQPKKKLRKCADSQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKL5</accession>
    <name>W5NKL5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKL5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021174"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021210.1">
        <property type="protein sequence ID" value="ENSLOCP00000021174.1"/>
        <property type="gene ID" value="ENSLOCG00000017132.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162064"/>
    <dbReference type="HOGENOM" id="CLU_001570_22_1_1"/>
    <dbReference type="InParanoid" id="W5NKL5"/>
    <dbReference type="OMA" id="RTHIHAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017132">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF153">
        <property type="entry name" value="CYTOCHROME P450 2G1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="156" mass="17480" checksum="971ABE2419FCEA99" modified="2014-04-16" version="1">SGMGLTSCLVLVAAVLAVIGFLTRSRRNPRRRLPPGPTPLPLLGNILQMDKDAPFESFVRLSERYGPVLTVYLGGQRTVVLVGYEAVRDALLQQSDDFTGRAPFPFLQRLTKGYGLAISNGRRWEQLRGFTVNTLRDLGMGRRRLEEQILEESQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLM6</accession>
    <name>W5NLM6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLM6"/>
    <dbReference type="FunCoup" id="W5NLM6">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021535"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021572.1">
        <property type="protein sequence ID" value="ENSLOCP00000021535.1"/>
        <property type="gene ID" value="ENSLOCG00000017430.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102682539"/>
    <dbReference type="eggNOG" id="ENOG502RP8U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154762"/>
    <dbReference type="HOGENOM" id="CLU_076370_1_2_1"/>
    <dbReference type="InParanoid" id="W5NLM6"/>
    <dbReference type="OMA" id="NCPPKYE"/>
    <dbReference type="OrthoDB" id="8830244at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017430">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="22624" checksum="69C4FD8AF849A86F" modified="2014-04-16" version="1">MASVGIQILGITLSMIGWLGVIIVCALPMWRVTAFIGANIVTAQIIWEGLWMNCVVQSTGQMQCKVYDSMLILPQDLQAARAMIVISIVVGIVGVLLSVAGGKCTNCIEDEATKAKVCILSGVIFIIAGVLCLIPVCWSANTIIRDFYNPLLTEAQRRELGASLYIGWGAAALLLLGGGLLCWNCPPKENQNYRGRFTPVQSTATSKNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM02</accession>
    <name>W5NM02_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zgc:172079</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NM02"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021698.1">
        <property type="protein sequence ID" value="ENSLOCP00000021661.1"/>
        <property type="gene ID" value="ENSLOCG00000017556.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWDG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NM02"/>
    <dbReference type="OMA" id="KANMYGS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017556">
        <property type="expression patterns" value="Expressed in mesonephros and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14982">
        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000142">
        <property type="entry name" value="G protein-coupled receptor 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF112">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="33"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="36387" checksum="01B30BBA1F7CAA86" modified="2014-04-16" version="1">LGGATMDNTTTLHSANWVFVGTYAVVFVAGLTLNLIALVVFFRHKKTRSHTTTYMTNLALADLLLVSTLPIRIYHHSGGSIQNQKMCETVGLILLVNMYGSIFLLTCISFDRCIAVCFPMSLRIKEHRKKAPLICLGVWILTIGASVPIYLSKNSTPTDGNITQSNKCFSSRPIYATQPLAITTTLTVGFGIPLFSMLVCSWCLIRAINKSTVAQTDLVNKKKIRRMIAANLAIFLLCFLPYHAMLFVLFLKRNTEQIEDNLLTVYQYSLMIACLNAMLDPLAYYFTTETFRTRVDVQNLKKKVWIFYSNSSDGQNRPHSPLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMA6</accession>
    <name>W5NMA6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="7918.ENSLOCP00000021765"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021802.1">
        <property type="protein sequence ID" value="ENSLOCP00000021765.1"/>
        <property type="gene ID" value="ENSLOCG00000017660.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287361"/>
    <dbReference type="InParanoid" id="W5NMA6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017660">
        <property type="expression patterns" value="Expressed in mesonephros and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46148">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46148:SF52">
        <property type="entry name" value="OS04G0603800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="230" mass="25856" checksum="BF706226DA2B049B" modified="2025-10-08" version="2">MXXXXXXXXXXXXHRRPAPRFRKGQFVWLSTRNLPIHQPSKKLGPRYIGPLQNLSRITPVTFRLALPPTLRIHSLFHVSLLKPVYHSPLAAPQHRPPPPRRIQGRPAYTVHKIIDSRWNRGTLQYLVDWEGFGAEERSWVPEKDILDPTLISDFHRDYPNQPSRASGAAREGGGTVTTTSQLRSTSEPISTSNGLLLTSAATRVNTPAIALPCGTPGYLYHSYLIPVARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMK8</accession>
    <name>W5NMK8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMK8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021905.1">
        <property type="protein sequence ID" value="ENSLOCP00000021867.1"/>
        <property type="gene ID" value="ENSLOCG00000017763.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182933"/>
    <dbReference type="HOGENOM" id="CLU_068368_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMK8"/>
    <dbReference type="OMA" id="CLLPFDR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017763">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047123">
        <property type="entry name" value="MYADM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068:SF3">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER MYADM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="18"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="151"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="292" mass="31584" checksum="29FA3CB8FCE1D662" modified="2014-04-16" version="1">PREAPPGVMPVILGEVSVLTTPLSLVRLLAITFGCAVFSLVAADDPGGSPFKIFCMFTWCFFFTMTVLILLVEFIQFQSLVPLSWKNLPITVATLAALMTLTASIGFPWLVLPPAVSGRMVAATVFSCLTFLAYITEAYLLKVGSSEQRGYMASAPGLLKVFQVFGGCVIFVAVVDHDRRAAYFWWCLAVYCCCFLMSLGTILVMLGDCAGRCPLPFSRVLAAFSSLSVLLYVTATVFWVTAVLGPGPQNQNPNPNPSQPRPGLIMSTVMTCLNLLAYTVDLAFSIKLCARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMX1</accession>
    <name>W5NMX1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Chemokine (C-C motif) receptor 6a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213153.1">
        <property type="nucleotide sequence ID" value="XM_015357667.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213164.1">
        <property type="nucleotide sequence ID" value="XM_015357678.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMX1"/>
    <dbReference type="FunCoup" id="W5NMX1">
        <property type="interactions" value="743"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021980"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022019.1">
        <property type="protein sequence ID" value="ENSLOCP00000021980.1"/>
        <property type="gene ID" value="ENSLOCG00000017877.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692258"/>
    <dbReference type="KEGG" id="loc:102692258"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234667"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_3_1"/>
    <dbReference type="InParanoid" id="W5NMX1"/>
    <dbReference type="OMA" id="HIICAAV"/>
    <dbReference type="OrthoDB" id="9828427at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017877">
        <property type="expression patterns" value="Expressed in liver and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000035">
        <property type="entry name" value="C-C chemokine receptor type 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001277">
        <property type="entry name" value="CXCR4/ACKR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF611">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00645">
        <property type="entry name" value="CXCCHMKINER4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="47"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="41643" checksum="4D0F7A891FEA01B3" modified="2014-04-16" version="1">MTSEPYTTTSFDYSDIVFPCDHKEARDLHSDIIVYVNSIICVLGLLGNTLVIVTYAFYKKPKSMTDVYLLNVAIADILFVIALPLIIYNEQYQWHMGKWACKLLRGIYSINLYSGMLLLACISTDRYIAIVQARRSFRLRSTTLVYSHIICAAVWMVAITVSTPTFIYYDKYDIHVGDGDDIPVCENRFDSNDTAVLMKTLMPSMQISIGFFFPLLIMVFCYSSIIVTLLQAKNFQRHKAVRVVMAVVVVFIVCHLPYNIMLLIHTVVKFKTDQQCDTEIERSKITTVLQCLAYFHCCLNPLLYAFIGVKFRNHFRKIMQDLWCLGKRYISVRRTSRFTSEVFISRKSSEVSNAENASSFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN33</accession>
    <name>W5NN33_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Achaete-scute family bHLH transcription factor 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN33"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022081.1">
        <property type="protein sequence ID" value="ENSLOCP00000022042.1"/>
        <property type="gene ID" value="ENSLOCG00000017939.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163864"/>
    <dbReference type="HOGENOM" id="CLU_095919_0_0_1"/>
    <dbReference type="InParanoid" id="W5NN33"/>
    <dbReference type="OMA" id="GVYDCPF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017939">
        <property type="expression patterns" value="Expressed in zone of skin and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19747">
        <property type="entry name" value="bHLH_TS_ASCL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000038">
        <property type="entry name" value="achaete-scute homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF108">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="100"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="23487" checksum="6FAA9DA8AFA6A1C2" modified="2014-04-16" version="1">MNASFSHALMERRPGMMPGSCVQYGIAPPGGHSHSEHHHHAHHRCHPPAGPGDTLAHVPFLLYPANMDPSYYEASYGGALFPYVPFHGHFGVYDCPFEPAFIQKRNERERQRVKCVNEGYARLRDHLPGAVSEKRLSKVETLRAAIRYIKYLQDLVSRGAQAGAEAEERSGKGPRNATPTPAEAGVKRDCNSDGESQNSSSSDSPYCESEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNA7</accession>
    <name>W5NNA7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Binds calmodulin. Interacts with NDUFAF3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDUFAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNA7"/>
    <dbReference type="FunCoup" id="W5NNA7">
        <property type="interactions" value="868"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022116"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022157.1">
        <property type="protein sequence ID" value="ENSLOCP00000022116.1"/>
        <property type="gene ID" value="ENSLOCG00000018015.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001627"/>
    <dbReference type="HOGENOM" id="CLU_2432596_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNA7"/>
    <dbReference type="OMA" id="EECRPCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018015">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009622">
        <property type="entry name" value="NDUFAF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13338:SF4">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13338">
        <property type="entry name" value="UPF0240 PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UPF0240"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="91" mass="10443" checksum="B673D8E5A19F7CB0" modified="2014-04-16" version="1">KNEEECRPCKSRVVGNPYGILDIKDIPKGKLSIVEALTVLNNYKHSPKSWTPNKIAQEYSLDLKDTKALLEFFILFDVKIIPPKTEDKKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNU7</accession>
    <name>W5NNU7_LEPOC</name>
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            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000022306"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022347.1">
        <property type="protein sequence ID" value="ENSLOCP00000022306.1"/>
        <property type="gene ID" value="ENSLOCG00000018205.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_077059_0_0_1"/>
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        <property type="component" value="Linkage group LG3"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
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    </dbReference>
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        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
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    </dbReference>
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        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1-LIKE-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="47"/>
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    </feature>
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        <location>
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            <end position="80"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="159"/>
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            <end position="289"/>
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        <location>
            <begin position="38"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="35865" checksum="161DCFFADBB74A1F" modified="2014-04-16" version="1">NATQNNVSTEDIFLIPSLGVRQLRMVVVQVLVAIFLYVNCFMLFTFFKTEIFRTTTRYILFAHSLLSDSVLLFLTDLVVLQVHFNILLPVEFCILLCMIMEIVSNTTPFTITAMCLERYVAICLPLRHADISTLRRTLVGILFIWALSSIYAIIDLFIFTATAPPSYFIEDTFCSYEILLLTEWHSYTRAILSNLEFVVIGIILVFCYIKIMSAARAASGDDTKSSSKAQKTLLLHTMQLLLCLIELLCPFVEMAVLEIDIHIFVTVRYFNFIAFTLASRCLSPLVYGLRDAEFFNALKNYACLRLKKTVSPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVJ5</accession>
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        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVJ5"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000000152.1">
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        <property type="gene ID" value="ENSLOCG00000000139.1"/>
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    </dbReference>
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    <dbReference type="InParanoid" id="W5LVJ5"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000139">
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="174" mass="19013" checksum="BEDC4CE3C88837A3" modified="2014-04-16" version="1">TLQSKTGCKMFTFCIIIMMATEPTATMTFITIFIWTLLIYTQESSGQVTVAQTPSVRSVLSGQTVSVSCKTSPAVYNNNYLAWYQQKAGEAPKLLIYYATTRQTGIPERFSGSGSGTDFTLTITGVQTEDAADYYCQSVHNINSYWPRTQCYTAVQKPPSAGLHSNCSAAAGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVZ5</accession>
    <name>W5LVZ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVZ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000302"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000302.1">
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        <property type="gene ID" value="ENSLOCG00000000276.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LVZ5"/>
    <dbReference type="OMA" id="HYISSTQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000276">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001897">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="21"/>
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            <begin position="22"/>
            <end position="120"/>
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            <begin position="23"/>
            <end position="120"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="120" mass="12953" checksum="55DBD2E09687858B" modified="2014-04-16" version="1">MTFITVFIWTLLIFSFSESSGQVTVTQTPAVKSAAPGQTVTVSCKTSPAVYGNNWLNWYQQKAGEAPKLLIYGAATRQSGIPEHFSGSGSGTDFTLTITGVQVEDAADYYCMGHHNLNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWW4</accession>
    <name>W5LWW4_LEPOC</name>
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        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Lepisosteus</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="W5LWW4"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000000623.1">
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    </dbReference>
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    <dbReference type="OMA" id="RIYPGHI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000564">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:immunoglobulin mediated immune response"/>
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        <property type="entry name" value="Immunoglobulin heavy variable 1-4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="IgHV"/>
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            <begin position="33"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13011" checksum="0330C807A573A9F7" modified="2014-04-16" version="1">MQVLACLMLLFLSVKELHCVQLTQSASEIGKPGQSLRLSCQVSGYSLTDNSYATHWIRQPPGKGLEWLGHYYTSGNSYAPSVQGRFTISTDSGSTVYLEMTSLKAEDSGVYYCARESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX43</accession>
    <name>W5LX43_LEPOC</name>
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        <submittedName>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LX43"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000700"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000703.1">
        <property type="protein sequence ID" value="ENSLOCP00000000700.1"/>
        <property type="gene ID" value="ENSLOCG00000000634.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_1_2_1"/>
    <dbReference type="InParanoid" id="W5LX43"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000634">
        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:immunoglobulin complex"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:immunoglobulin mediated immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig_V-set"/>
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    </dbReference>
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        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="215" mass="23909" checksum="B4684C2CD97BE445" modified="2014-04-16" version="1">VLTESEPSVLQPGGSHRLTCTASGFTFTSYDMGWVRQAPGKAMYSVYKLCASGGSTYYSQSVQGRFTISRDNSKNQLYLQMSSLKTEDTAVYYCAKDPQFLHELLGLTLIIMQSGVRSQIQLTESEPAVKKPGESHTLSCKTSGFTFGNYYMSWIRQAPGKGLEWVAHISYWAGTDIKYGSAVQGRFTISRDNPKSTLNLQMNSLKTEDTAVYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXJ3</accession>
    <name>W5LXJ3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="W5LXJ3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000850"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000854.1">
        <property type="protein sequence ID" value="ENSLOCP00000000850.1"/>
        <property type="gene ID" value="ENSLOCG00000000769.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00560000077365"/>
    <dbReference type="HOGENOM" id="CLU_2346089_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXJ3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000769">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="7">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="97" mass="10903" checksum="F999F8CA2B121F5E" modified="2014-04-16" version="1">MEVGSEAAEWVSTHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSPTTHDKMDCKGIETVRRDNCPLVVSLINACLQSILIDRCGSPTDSGVPFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXU0</accession>
    <name>W5LXU0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069043247.1">
        <property type="nucleotide sequence ID" value="XM_069187146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXU0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000947"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000951.1">
        <property type="protein sequence ID" value="ENSLOCP00000000947.1"/>
        <property type="gene ID" value="ENSLOCG00000000853.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697609"/>
    <dbReference type="KEGG" id="loc:102697609"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222287"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_6_1"/>
    <dbReference type="InParanoid" id="W5LXU0"/>
    <dbReference type="OMA" id="LLRCNYR"/>
    <dbReference type="OrthoDB" id="10063595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000853">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15329">
        <property type="entry name" value="7tmA_5-HT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000071">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF199">
        <property type="entry name" value="IP13425P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="93"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="434" mass="48729" checksum="E98539D509CADDC8" modified="2014-04-16" version="1">MVMHDSPTLILNSQLSLGEEQDLAPWQGLKPTALVTDGPSAVAFKPTVQERLTGLGNGTNSTELSGDVLLYGDVEKVIIGIVLSIITLTTISGNSLVIISVCIVKKLRQPSNYLVVSLAAADLSVALAVMPFVIITDLVGGKWLFGTFFCNVFIAMDVMCCTASIMTLCVISVDRYLGITRPLTYPVRQNGKLMAKMVFIVWLLSASITLPPLFGWATNVTEERVCLISQDFGYTVYSTAVAFYIPMTVMLIMYYRIYKAAKVSAEKHKFMCVPRQYAHQGVYSTEQEPQVRPHRRLSRRDRVLEECATLSKLLRHDGHNISIFKREQKAARTLGIIVGAFTFCWLPFFLLSTARPFICGITCSCIPLRLERTLLWLGYANSLINPLIYAFFNRDLRTTFWNLLRCRYRNINRRLSAASMHEALKVKERPDAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZW0</accession>
    <name>W5LZW0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Beta-secretase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase A1 family.</text>
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    <dbReference type="EMBL" id="AHAT01003351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01003352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5LZW0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001667"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001672.1">
        <property type="protein sequence ID" value="ENSLOCP00000001667.1"/>
        <property type="gene ID" value="ENSLOCG00000001455.1"/>
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    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159548"/>
    <dbReference type="HOGENOM" id="CLU_039009_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZW0"/>
    <dbReference type="OMA" id="CDTVNDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
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    <dbReference type="Bgee" id="ENSLOCG00000001455">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0050435">
        <property type="term" value="P:amyloid-beta metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0097324">
        <property type="term" value="P:melanocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
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        <property type="entry name" value="beta_secretase_like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Aspartic_peptidase_A1"/>
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        <property type="entry name" value="Aspartic_peptidase_AS"/>
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        <property type="entry name" value="BACE"/>
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        <property type="entry name" value="BACE2"/>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
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        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
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        <property type="entry name" value="BETA-SECRETASE 2"/>
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        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BACE2"/>
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        <property type="entry name" value="BACEFAMILY"/>
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        <property type="entry name" value="PEPSIN"/>
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    </dbReference>
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        <property type="entry name" value="ASP_PROTEASE"/>
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        <property type="entry name" value="PEPTIDASE_A1"/>
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    </dbReference>
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    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="chain" id="PRO_5004865408" evidence="17">
        <location>
            <begin position="20"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="456"/>
            <end position="481"/>
        </location>
    </feature>
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        <location>
            <begin position="80"/>
            <end position="418"/>
        </location>
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        <location>
            <position position="98"/>
        </location>
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    <feature type="active site" evidence="12">
        <location>
            <position position="292"/>
        </location>
    </feature>
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        <location>
            <position position="355"/>
        </location>
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        <location>
            <begin position="221"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
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            <end position="382"/>
        </location>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="506" mass="54819" checksum="177ABC3849489494" modified="2014-04-16" version="1">MRNTLRVLWLVLFSRTCCSHLTFPLKLFAGKFNSSVDLDLTPLQKKNATSGGLSLASDPTGQVNFLDMLGNLNGDSGRGYYIEMLIGTPPQTLNILVDTGSSNFAVAGARDPYISRFFNSSLSSTYWPSGRTVSVRYTQGSWVGELGTDLISITKGTISAITINIATILSSEGFFLPGVKWQGILGLAYPVLARPDSSVTPFFNSVVRQTGIPDIFSLQMCGAGVSGGSDADAARGSLIMGGIEPLLYQGSVWYTPVKEEWYYQVEILKLEVGNQNLNLDCKQYNADKAIVDSGTTLLRLPQDVFRAVVDAIVREALIQDFSDGFWTGTKLACWMKGETPWPFFPKISIYLRAMNASQSFSITILPQLYIQPITEVGNALECYRFGISSSSSGLVIGATVMEGFYVIFDRALSRVGFALSTCAGSSGSPVSEIAGPFSALDVSSDCVSVPVLQEPVLWIISYALMGVCAIALLVLIILLLLPCRCRRGDDPGEITDASSLVRNRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M010</accession>
    <name>W5M010_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4515 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF14988"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="271" mass="31867" checksum="1FF04485BB69B5BD" modified="2014-04-16" version="1">KMPKKKADSGPKLKTSEGDGADEAQRPATDKEVLLQKEYEQLTEALNSLRRKVEHLRRENEYLQQEAHQTRMESQEYMSYMSKRTQKRQNAIISLSDQNQVELEKLRRQREELLDTHKGQSTELKRQILEKENELSVLNLEISDMQEYKSLQQQQLGRIQELEQEVLTMRARHSESLQHLKAGFLGNKVQYESQARHEVQALAVAANREASRCLAAHTQQVAQENRQLRGELLQLIQRARDLRLHQGRLQEQGQQLLRERQYVQDLRRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0P3</accession>
    <name>W5M0P3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sacsin molecular chaperone</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001950"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001955.1">
        <property type="protein sequence ID" value="ENSLOCP00000001950.1"/>
        <property type="gene ID" value="ENSLOCG00000001682.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQPY">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG3"/>
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        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
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        <property type="term" value="F:Hsp70 protein binding"/>
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        <property type="entry name" value="DnaJ domain"/>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="HATPase_C_sf"/>
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        <property type="entry name" value="HEPN_dom"/>
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        <property type="entry name" value="J_dom_sf"/>
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        <property type="entry name" value="SACS/Nov_dom"/>
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        <property type="entry name" value="Sacsin_chaperone_reg"/>
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    <dbReference type="InterPro" id="IPR000626">
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBIQUITIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="4317"/>
            <end position="4392"/>
        </location>
    </feature>
    <feature type="domain" description="HEPN" evidence="5">
        <location>
            <begin position="4462"/>
            <end position="4578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="4262"/>
            <end position="4307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4356"/>
            <end position="4383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="4268"/>
            <end position="4278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="4279"/>
            <end position="4291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="4590" mass="520385" checksum="D6A00B49C6BA7109" modified="2014-04-16" version="1">[encoded data, 4590 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M117</accession>
    <name>W5M117_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cystein proteinase inhibitor protein salarin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Inhibits papain and ficin (cysteine proteinases) but not trypsin (a serine proteinase).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011236">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000002079.1">
        <property type="protein sequence ID" value="ENSLOCP00000002074.1"/>
        <property type="gene ID" value="ENSLOCG00000001788.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:lysosome"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cathepsin L"/>
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    </dbReference>
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        <property type="entry name" value="Cathepsin L1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
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        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018714810" description="Cystein proteinase inhibitor protein salarin" evidence="13">
        <location>
            <begin position="27"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="15">
        <location>
            <begin position="35"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="14">
        <location>
            <begin position="129"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="286" mass="32255" checksum="C655061B33967F43" modified="2014-04-16" version="1">FLSSRFTMKAVIVLASVVVCLHLGTCIDPSLDLEWENWKMKHKKVTRRRAVDQRRREIWEENYRAIEDHNKRYREGKETYEMGMNQFGDLTTEEFVKLTKMRTLTRMALRRSTKVMSATDLRLAASKLNFTGIDYRTMGYVTPVQNQGTCGSCYAFSAAGALEAQWKKKTGTLIPLSKQQLVDCSTSVGNAGCRGGLARLSYDYIIKNRGIQAEATYPYAMQKKNCTADKSKIVATVSDWKFLPVGDEQALQDALVTIGPVAVAIDGSTIKFQFYRKGIFSDSKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1U3</accession>
    <name>W5M1U3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hippocalcin-like protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1U3"/>
    <dbReference type="FunCoup" id="W5M1U3">
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        <source>
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    <sequence length="266" mass="31009" checksum="FE4AD29CDEF09BE5" modified="2014-04-16" version="1">MTWLSRSGERSIHFLVNGEARRSRKFRLFLVQYSYLKPGDLCLFKVCQSRKKDYPRCPQQVKGNRHFTGRSGRCTMGKHNSKLAPEVLDDLTKSTEFNESELKQWYKGFLKDCPTGILNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRMEMLEIIEAIYKMVGTVIMMRMNEDGLTPQQRVDKIFSKMDKDHNDEITLEEFKEAAKSDPSIVLLLQCDMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2D1</accession>
    <name>W5M2D1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ADAM metallopeptidase with thrombospondin type 1 motif 13</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021666">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2D1"/>
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        <property type="interactions" value="372"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000002539.1"/>
        <property type="gene ID" value="ENSLOCG00000002168.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_000660_10_1_1"/>
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        <property type="component" value="Linkage group LG21"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in liver and 10 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF20">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS_CR_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="17">
        <location>
            <begin position="124"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1053"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1053"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1071"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 15">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
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    </feature>
    <feature type="disulfide bond" evidence="14">
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    </feature>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="320"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1153" mass="123277" checksum="ECFEA68CD29D95D0" modified="2014-04-16" version="1">RLRRTAVGLDVRHLELLVAVGPDVKDVHRQDTERYVLTNLNIVSPLPGQGQGWREGRSRGGRGLDLRSSSPAPTLLLCVSTGLGAGSGSGQRSAPLGDPLFLCYPCTPCRFDLELPDGNKQVRGVAQLGGACSSGWSCVITEDTGFDLGITIAHEIGHSLGISHDGPGTSCSGSGFIMAADGGYNSVDLAWSPCSREQFLSFARCVPRRSGPPATGPAEETPPSPVWDDQCRIAFGSEARACTFRHGGIDMCRVLSCHVTPNDHSSCSRLLVPLLDGSECGPNQWCLKGRCVSPTQLSSPAMIHGAWSGWSEFSQCSRTCGGGVRSRRRQCNNPRPAFGGRSCEGRDVEAGLCNTQACEKSQLDFMAEQCSRTNPEPLRLKPTSASYHSWVPAVGFVSGDAQCKLMCRSLGQNFMMSRGSQFLDGTRCEPGSPTPAGSRGACLAGRCQLFGCDGQLNSGTVEDVCGVCGGNGTSCSQVSGSYSEGQPKEYTTFLTLPANATRVHVVNSRSLFSHLAVTVGERYVLAGRGAVSRNTSYPSPLEDGRLEYRLHLTADHLPDSEELLLPGPVQERVDIQVYRKYGREYGEVTSPNISYSFFIPWEIPSAKWTAATSACSTTCGTGVQEISYVCVDSSTEQRAEDKECGSPPQPAVLQTPCNLRPCPPSWEVGEFGPCSAPCGRGEMVRPVWCVRREGGLTVQLPASECPPRWAPRSSQPCNPQPCPGRWRISDPGRCSAVCGPGVAGRSVSCVQALRGSDVEVDERLCPESERPAELLPCVVDVCPLGWESGNWSEAPRLHKAGLDWVSRARAGPVYVWSPVIGQCSKSCGGGLAEVRYSCVDHQSRSTVPETQCDEASKPGSRLEPCGRTPCPPAWHSKMGVCSVSCGGGVARRLLYCTRASEEAGEVLSDSECGAVPRPEEVVACNTHSCPARWRVQESGPCSVSCGLGAARRTVSCVRFEQGRDSEVPPELCPELEKPPALVPCLVQVCTFSWAVQEWTKCSVSCGFGIQSRQVSCVGPATPHPLSPLFCMHLPKPITIQGCHLQDCPDLAVTRPGATPAPSRPTATPSSPREKPQYREEPLSPPKTETILCVTLTVSQPALPAPGVCGQLLLKDSGTVDLRHVQSRDCLLSIGRPLGQVIQVKVVSSSLNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2U2</accession>
    <name>W5M2U2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Piezo type mechanosensitive ion channel component 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002700"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002706.1">
        <property type="protein sequence ID" value="ENSLOCP00000002700.1"/>
        <property type="gene ID" value="ENSLOCG00000002260.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1893">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154456"/>
    <dbReference type="InParanoid" id="W5M2U2"/>
    <dbReference type="OMA" id="KSCWLIQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002260">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF6">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="682"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="688"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="719"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="771"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="911"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="937"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="961"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="1095"/>
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        <location>
            <begin position="1119"/>
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            <begin position="1147"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1203"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1266"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1291"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1326"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2037"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2066"/>
            <end position="2085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2091"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2318"/>
            <end position="2341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2361"/>
            <end position="2380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2389"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2423"/>
            <end position="2443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2455"/>
            <end position="2471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="2510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2791"/>
            <end position="2814"/>
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    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="15">
        <location>
            <begin position="26"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="15">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1245"/>
            <end position="1607"/>
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    </feature>
    <feature type="domain" description="Piezo transmembrane helical unit" evidence="14">
        <location>
            <begin position="2046"/>
            <end position="2167"/>
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    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="16">
        <location>
            <begin position="2316"/>
            <end position="2553"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
        <location>
            <begin position="2591"/>
            <end position="2878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="837"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1463"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1740"/>
            <end position="1766"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1788"/>
            <end position="1920"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2171"/>
            <end position="2197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2216"/>
            <end position="2264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="849"/>
            <end position="869"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1464"/>
            <end position="1473"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1816"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1833"/>
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        <location>
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        <location>
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        <location>
            <begin position="2248"/>
            <end position="2261"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2881" mass="331003" checksum="BA3A78F10D0B9C31" modified="2014-04-16" version="1">MASEVMCGLIFRLLLPVCLAAACLFRYNGLSFVYLIYLLLIPLLSEPTQATMQGHTGRLLKSLCFTSLTFLLLHIIFQITVNSLVAGNSIDSGFNCSTWEKSIRQIGFESVRGADAGNGVRVFIPDIGMFIAGLSTWLLCRSLVQNNPKEDMAQYNEDFETEDQEEEKLEEDDELMFEEDFEPDLEPHDEELEEDEEEEEEQEESTKMKILRRIAGVASKLKEIIGNLITTAGKVVVTILLGLTGIMLPSLTSAVYFLVFLGLCTWWSLCRTFDQLVFSCLCVLMAIYSAGHLLVLYLYQFQFFQELVPPHDNYTSWFGISSVIQTNCSYTWMIIANHELEWYHYVSPIMILVLYYTLATLIRLWLQEPTDEMYYDDDEEEKNETEEDEKEIMCNEMASTAEKKRQIWRNAHCNTEERNLLSTQDGYSTSDVPLVTANGTTIDYHNLSTPLAIENGPRQLDTYSTPQYRIDQVDDEPEKKEDSLSEVLEDADEMSEEIENPKVNALVTIFRFIMKQSYICALIAMMAWSITYVSWLTFVFLIWSCTLWMVRDRRKYAMLTSPFMVFYGNLLLILQYIWSIKPIEPVSGLFLKKGVPFQELGTKILCLLSFWLLLRQHLMEKQEKLQEEAVLTDVRVDSQKTEEEEDEENGGNKEIMQVLGNMVMGMFVKYWIYICGGMFFFVSFEGKIVMYKIIYMMLFLFCVALYQVHYDWWRRILKYFWMSVVVYTMLVLVLVYTFQFDSSLRVWSNMTGLNKEKLEDLGLEQFTVSALFTRIFIPTCFLLVCILHLHYFHERFLQLTDLKAVAAKQDCTVYRLVHQDGSLADITMLSSSLNPARKEEEEDMSQEAGGEKPNEKELLDEGSKGESRHCSVGTETTTEDTSDLKNKWHLVIDRLTVLFLKFLEYFHKLQLFIWWLLELHIIKIVSSYIIWVTVKEVSLFNYAFLICWAFALPYSQFRPLASSICTVWTCVIIVCKMLYQLGSIQPPTYSKNCSMPVNYTDEQKKEMSTSLLYNGSIDPTNWVGLKKSDPLLGYLRNNLLMLAILAFEVTIYRHQEYYRLRNNLTAPVTKTIFHDITRQHLDDGILNCAKYFINYFFYKFGLETCFLMAINVIGQRMDFYAMLHAFCLIAVMYRRRRKAIAEIWPKYCYFLACMLTFQYFLCIGIPPAACKDYPWRFPSSTTGSNVIKWLYIPDFHTQPNPLFLVYDFMLLLCASLQRHVFEDENRAAVRIMAGDNVEICRDLDAASFSQHNPVPDFIHCRSYLDMLKVIMFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILHYWDFLIAYNVFVITMKNVLSIVACGYINALVENNCWLIQLFSLACTIKDYSRPDSSGKCALPSDEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIRSSQILASRGAELFQATIIKAVRARIEEEKKSMDQLKRQMDRIKTRQQKFKKGKERMLSLAQQSGEGQNMMKEEEEDDGEADKEKAKGLKRQWWRPWVDHASMVRSGDYYLFETDSEEEEEEDKKEEEPHKKSAFQRAIGKFASAILALPKSIIKLPKTILQYVIRAAKFVYHAWITDSKTALKERDKDKRKFWKRYTSKGKWRRGDKEGQVAIECGEREDETESMEEGKKSEGPDNIIKRIFNIVKFTWVLFLALLDSFTAWLNSICREHIDISTVLRIERCMLTREIKKGNVPSRESIHVYYQKQMRMNFSRESSLDRIDEDLSGSSSHKAERKRGAHRMDSLDSVGSRDTLSSVYTEATMLFSRQSTLDDLDGPSESIPKTSERARPKLRKMYSMDMSSSSADSASVVSSEPTQCTMLYSRQGTTDTIEEVEDEQEEEAAPPGSLGPGADSDGAPGAEAGDGEPGPGEPASEAGGKEERSQGEESEWPGGAEPPLREEGAGEHLYPAECEVQLYSPDTDAPATYPTDADADVPPSYSKAVSFDRMSISSGEGPDDKHLMAMTPDDSKSDNAVDALLPSLTHELTASELLLNKMFYDEELESSERFYRTQPQILQLLYALYNMLVAHSEMVCYFVIILNHMISASMVTLVLPILIFLWAMLSVPRPSKRFWMTAIVYTEVTIVIKYFFQFGFFPFNKNVEVDRSKPYHPPNIIGVEKKEGYVHYDLVQLLALFFHRSILKCHGLWDEDDGGVAEKATHRDDSEDEGSVSAERRGSAGSLKSINLAASLESVHVHFPEPQTPVRRKSSSAGSQISHRSSRSSGRSKRGSTSTRNSSRKGSSVLSVQQKTRKELILEKLKEQLIIAKAFTIKKTLEVYIPIRRFFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAADITSSLSEDQVPEAFLVMVLIQFGTMVVDRALYLRKTVTGKVIFQVILVFGIHFWMFFILPGVTERRFSQNTVAQLWYFVKCIYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFVLKCWRESEKRYPQPRGQKKKKVVKYGMGGMIVVLLICIVWFPLLFMSLVKSVAGVVNKPLDVSIKITLGGFQPIFTMSAQQNQLKNLSSDDFNKLLGEYESSAGAMQFLEAYTHEDVTVAELEGSSNALWTISPPSRDNLIKMLSNNNTFPVTVFWTIQRNMSLGAKVETAQDKHINYLDQSTKQELIALLNGTRKTAVLIDNVFPCFIRAPSDSNAKPIQQLYLDKAYKNITLRLERKSNDTEEIQEWWIINQTSRLSIQKSDHSSMTGLEMFVFSDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M387</accession>
    <name>W5M387_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">28S ribosomal protein S14, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M387"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002845"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002851.1">
        <property type="protein sequence ID" value="ENSLOCP00000002845.1"/>
        <property type="gene ID" value="ENSLOCG00000002424.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015663"/>
    <dbReference type="HOGENOM" id="CLU_139869_1_0_1"/>
    <dbReference type="InParanoid" id="W5M387"/>
    <dbReference type="OMA" id="FGLCRNQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002424">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="14126" checksum="9D1D747EC8C85224" modified="2014-04-16" version="1">SCQTLRSSRGNVEQARGYYVDWRMLRDVKRRQMAFEYANDRLRINAIRKNTILPKELQEVADKEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHNQMSGIQRAMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4D3</accession>
    <name>W5M4D3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01039639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4D3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003241"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003248.1">
        <property type="protein sequence ID" value="ENSLOCP00000003241.1"/>
        <property type="gene ID" value="ENSLOCG00000002756.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZ7S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000024060"/>
    <dbReference type="HOGENOM" id="CLU_551610_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4D3"/>
    <dbReference type="OMA" id="CANEIPG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37557">
        <property type="entry name" value="115 KDA PROTEIN IN TYPE-1 RETROTRANSPOSABLE ELEMENT R1DM-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37557:SF4">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="58057" checksum="248F85B03E11ED24" modified="2014-04-16" version="1">MTLAFADDLVLLSNNWEGMKKNTDTFEVFCAHTGLMVRAKKCHGFLISLTKDSYVINNYEDWSINNNKLHTKYLDLKINPWTTTKKPDAVNLLQDWIHKIYKAPLKPSQKQILNVYTIPRLIYQMDHAYTNQLSLKNLHGLIRKAIKRWLHLPESTCNGLLYSSNRDGGLGSTNLSNLIPSIQIRRLQRMAMSSNPIISTLMRSEEAQVKLQNLWIQAGGDENKILQLKDPTTLTVQSEDILLPVEWEPSPNQKPKFMTPCNWRNDEFENWTKLPVQGTRIKNFKDDPDSNSWLAHHKGFKERHYIAALQLRTNVYPNRESLNRGQRDTQHCYKCQQKTETYSHILDQCPCGKSTRIKRHNKICYILAKEAKKQNWEIRKEPHFRLKEVRKPDLIFTKNNESIVIDVTVRYEQNLHTLKEAEREKVVYYEPLKEQIKETTGAEKVTFYGFPIGARGKWHNRNFNVLQSLQIRESRQKQLFSRRALLYSLDILSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4K4</accession>
    <name>W5M4K4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor C family, h1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4K4"/>
    <dbReference type="FunCoup" id="W5M4K4">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003312"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003319.1">
        <property type="protein sequence ID" value="ENSLOCP00000003312.1"/>
        <property type="gene ID" value="ENSLOCG00000002816.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="HOGENOM" id="CLU_005389_5_1_1"/>
    <dbReference type="InParanoid" id="W5M4K4"/>
    <dbReference type="OMA" id="ISIGYQI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000067">
        <property type="entry name" value="Olfactory receptor C family, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
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        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
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        <property type="entry name" value="GPCR_3_CS"/>
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        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
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        <property type="entry name" value="Peripla_BP_I"/>
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        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NCD3G"/>
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        <property type="entry name" value="VOMERONASL2R"/>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865540" evidence="13">
        <location>
            <begin position="19"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="594"/>
            <end position="619"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="631"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="664"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="709"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="752"/>
            <end position="775"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="787"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="819"/>
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        <location>
            <begin position="594"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00007242"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50259"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003312.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="860" mass="95104" checksum="910F6424645CA125" modified="2014-04-16" version="1">HNNCEMLLYALVLIGAFATISEKPVCKMVGKPEFPVLSKDGDVIIGGAFSIHSKSKVQMTLFQNEPEPLGCSSINFREFRFAQTMIFAIEEINNSTDLLPETSLGYRIYDSCGSIPSSIKTAMALMNGQEKTVLNESCTESGTVHAIIGESESSSTIAMATTTGPFHIPVISHFATCACLSNKKLFPTFFRTIPSDYYQSRALAQLVKHFGWTWVGAIRSDNDYGNNGMVTFVEAAQQEGVCIEYSEAILRTNPKEKIARVVDIIRKATSKVLVAFLAQGEMEVLLEEILLQNVTGLQWIGSESWITARQLATEKASEVLSGAIGFAINKSTIAGLKEFLLKIHPSQAPGSAILKEFWETAFGCTLSSQSKSNDTEHCTGTEQLALLRNQYTDISELRISNNVYKAVYALAHSIHNLLTCKDGQGSFTNETGTKNNTTEPRQVLHYLKGVNFTTRTGERVYFNTNGDPAAKYELVNWQHTRDGGTTFTTVGHYDASLPAGQQFTMNLVNIVWAGDHNQQPVSVCSESCLPGTRKVVQRGRPVCCFYCTPCAEGEISNSTDSIECTKCPLEYWSNEDQTQCILKETEFLSFGENMGIVLTMFSLAGACLNLAIGFIYFHFRATPLVKANNSELSFLLLFSLTLCFLCSLTFIGQPSDWSCMLRHTAFGITFALCISCVLGKTLVVLMAFKAKMPGNSVPKCSGPLQRLSVFTCTIIQVLLCILWLALSPPFPYKNMEYFKNKIILECDLGSTVAFYAVLGYIGFLSAMCFVLAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYISSPGKYTVAVEIFAILASSFGLLFCIFLPKCYIMLLKPEKNTRKHLIGKMHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M667</accession>
    <name>W5M667_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Splicing factor 3a, subunit 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631318.1">
        <property type="nucleotide sequence ID" value="XM_006631255.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M667"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003875"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003883.1">
        <property type="protein sequence ID" value="ENSLOCP00000003875.1"/>
        <property type="gene ID" value="ENSLOCG00000003273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687079"/>
    <dbReference type="KEGG" id="loc:102687079"/>
    <dbReference type="CTD" id="10946"/>
    <dbReference type="eggNOG" id="KOG2636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063402"/>
    <dbReference type="HOGENOM" id="CLU_027160_2_0_1"/>
    <dbReference type="InParanoid" id="W5M667"/>
    <dbReference type="OMA" id="QRCYHEE"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003273">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025086">
        <property type="entry name" value="SDE2/SF3A3_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13297">
        <property type="entry name" value="SDE2_2C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="11">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
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        <location>
            <begin position="342"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="501" mass="58787" checksum="01AC842C43004AB7" modified="2014-04-16" version="1">METILEQQRRYHEEKERLMDAKTKEMLHKKTTLRDQINSDHRTRAMLDRYMEVSANLRDSYEDKDGMRKEELSAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFEELMKARENPSEEAQNLVEFTDEEGYGRYLDLHDCYLKYINLKGAEKIEYITYLSTFDQLFDIAKERKNAEYKKYLEMLLEYLQDYTDRVKPLLDQNELFNKILNEFEKKWENGTFPGWPKETSSALTHAGAHLDLSAFSSWEELASLGLDRLKSALMALGLKCGGTLEERAQRLFGTKGMSLESLDPSLFAKNPKSKGPKKDTERNKEIGFLEAQIYEYVEILGEQRQLTHENVQRKQARTGEEREEEEEEQLSESESEDEDNEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKAFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKTLKASERWQPDTEEEYEDSSGNVVNKKTYEDLKRQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M689</accession>
    <name>W5M689_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase 33</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01008200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193529.1">
        <property type="nucleotide sequence ID" value="XM_015338043.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193530.1">
        <property type="nucleotide sequence ID" value="XM_015338044.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193531.1">
        <property type="nucleotide sequence ID" value="XM_015338045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M689"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003905.1">
        <property type="protein sequence ID" value="ENSLOCP00000003897.1"/>
        <property type="gene ID" value="ENSLOCG00000003301.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695433"/>
    <dbReference type="KEGG" id="loc:102695433"/>
    <dbReference type="CTD" id="65975"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159050"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="W5M689"/>
    <dbReference type="OMA" id="GQDNMMQ"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003301">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000557">
        <property type="entry name" value="Serine/threonine-protein kinase 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="30"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="317"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="485" mass="53649" checksum="19A61C32BE962986" modified="2014-04-16" version="1">MASQWTRSNSAENKVPHTRMEDEAAIQQIYTFGRKLGQGSFGVVHEASHIETQRKWAIKKVNREKAGSSAVKMLEREVTILKRVNHEHIIHLEEVFETPKRMYLVTELCEGGELKEILQKNKRFTENETRHIICSLAGAIAYLHKKDIVHRDLKLENILVKSNHNDNGNEMMLNIKVTDFGLAVQKGGVGSENMLQATCGTPVYMAPEVINAHDYSQQCDVWSIGVIMYMLLCGDPPFMATSEERLFELIKKGGLHFTDPVWKTISDGAKNVLQCLMKVDPAHRLTANELLDNPWITGDTSTPARPSNVLEMMKQFRDDPDDIDNGSLEDDLNGLSLKSSQEDAFQPRDVIDQRCSSAGSNGASDLNVEMDTDSGSSKPSTPTKQIQKKKTLTSSLPNGIVKKNGSTLKTCSTPSTGGKVSNLANTKRTTQQDKWESSPHSLGPAVRLASFSVKGELHKAPIPTSHSSGHKHSKHTAIAKSKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6E5</accession>
    <name>W5M6E5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641243.1">
        <property type="nucleotide sequence ID" value="XM_006641180.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6E5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003953"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003961.1">
        <property type="protein sequence ID" value="ENSLOCP00000003953.1"/>
        <property type="gene ID" value="ENSLOCG00000003348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693459"/>
    <dbReference type="KEGG" id="loc:102693459"/>
    <dbReference type="CTD" id="64785"/>
    <dbReference type="eggNOG" id="KOG1106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001622"/>
    <dbReference type="HOGENOM" id="CLU_081646_2_0_1"/>
    <dbReference type="InParanoid" id="W5M6E5"/>
    <dbReference type="OMA" id="IYKEGWR"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003348">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="8">
        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="94"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="211" mass="24043" checksum="C18FC2D73E8F1B4B" modified="2014-04-16" version="1">MATQSYTPVPPGVGMEDNFLSLDDILLSQEKLPCRTETAFPRLGFLEKSGDTGDIQEGTKMEMPLWLAKGLYDSKRRVVAVDLPKVYREGWRTVFGADPNVVDLHKMGPYYYGLGSQLLHFDSPENPDIAQTVLQTFVRRFRRIMDSSQNAYNEDTSALVEKLDCMERSLFRSGQAGLHDFQRWEKGQSSQMTASSLVQNYRKRKFAELEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6I8</accession>
    <name>W5M6I8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <dbReference type="EMBL" id="AHAT01019097">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5M6I8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003996"/>
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        <property type="component" value="Linkage group LG24"/>
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    <dbReference type="Bgee" id="ENSLOCG00000003379">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF26">
        <property type="entry name" value="PHOSPHOLIPASE A2 INHIBITOR CNF-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5004865713" description="UPAR/Ly6 domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="5">
        <location>
            <begin position="71"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="166" mass="17977" checksum="697F5FFEA98FBEB8" modified="2014-04-16" version="1">MTMSLLVKAVLFCGLLQPVSPLRCFYCSQFDSQPCREEEKDCSSSETSCLAATATTTFTAAPYKDTTPNGRQCFTCVDNNCTKKLNCLGNEDRCFHSSVVFAEGQASTVKGCLSQYACTKGALTVLDATLNGEVRCCKGSLCNSAWHAGQQLFLLLVSLASIQLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
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    <name>W5M6S6_LEPOC</name>
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        <submittedName>
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    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6S6"/>
    <dbReference type="FunCoup" id="W5M6S6">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004084"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004092.1">
        <property type="protein sequence ID" value="ENSLOCP00000004084.1"/>
        <property type="gene ID" value="ENSLOCG00000003445.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUEA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161353"/>
    <dbReference type="HOGENOM" id="CLU_001074_0_2_1"/>
    <dbReference type="InParanoid" id="W5M6S6"/>
    <dbReference type="OMA" id="FFQYYGN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1q_dom"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
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        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF27">
        <property type="entry name" value="COMPLEMENT C1Q TUMOR NECROSIS FACTOR-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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    <feature type="chain" id="PRO_5004867701" evidence="7">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="104"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="249" mass="26593" checksum="54AA13B1BD7FD77D" modified="2014-04-16" version="1">RMNPHQLWPLSLFIFLLVSSSNQIEDNQIPSLCSGQPGIPGSPGAHGSPGLPGRDGRDGRDAAPGEKGVKGDLGEPGMSGARGDRGDRGEPGEKGDRGQPGECAVAPKSAFSAKLSESRGAPTPVGEAVRFDVALLNEQGHYDAETGRFTCRVPGVYYFAVHATVYRASLQFDLMKNGHVVASYFQYYGNWPKPASLSGGSLLHLVPGDQVWVQMGLSEYSGFYSSQKTDSTFSGFLVYSDWKNSAVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7F2</accession>
    <name>W5M7F2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SH2 domain containing 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7F2"/>
    <dbReference type="FunCoup" id="W5M7F2">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000004318.1">
        <property type="protein sequence ID" value="ENSLOCP00000004310.1"/>
        <property type="gene ID" value="ENSLOCG00000003629.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXFM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048936"/>
    <dbReference type="InParanoid" id="W5M7F2"/>
    <dbReference type="OMA" id="QDCGPEG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003629">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13157">
        <property type="entry name" value="PTB_tensin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832:SF3">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832">
        <property type="entry name" value="SHC (SRC HOMOLOGY DOMAIN C-TERMINAL) ADAPTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="33"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="291"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="45580" checksum="06FEA248C662DDA7" modified="2014-04-16" version="1">ADHTGGMCETPTREASIVTRSAENALFVCFTSYVGSFPVEDCCLDEQTKLVHEQLQSLKDCNRRRPVSLKFSLSGLKMYDEDENVLLMAHALRRVSFSTCSPSEAQFAFVSHNPGCPDSQLYCHLFKGRHARAARFLNLLLCRCFQLVYLQKHPEEVQELSVSPIPDRPPMLLNQGISLNVSALVSFRRAPTQGGLVPSEKSQEPTPDEEQQSSPEENQVSSPTLVRKKAIRNKVLRSGAYRSFTYSSHTPRPSQDRRQAPQGRAWRSLDIQGSSTPTLAETEEALAEAVWSWAGLSSKGSSSLLLDDVLGAFLLHPQSGSSGCSLLTVRTHSGLANYNVKVNAEGKYFLENHLKNFDTIGALIEHYTEFQGELGCSLSSARVNHCYEWEEGPRGFKHSARPNETISISLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M806</accession>
    <name>W5M806_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01005101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221949.1">
        <property type="nucleotide sequence ID" value="XM_015366463.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221950.1">
        <property type="nucleotide sequence ID" value="XM_015366464.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221951.1">
        <property type="nucleotide sequence ID" value="XM_015366465.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069038188.1">
        <property type="nucleotide sequence ID" value="XM_069182087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M806"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004514"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004522.1">
        <property type="protein sequence ID" value="ENSLOCP00000004514.1"/>
        <property type="gene ID" value="ENSLOCG00000003771.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691768"/>
    <dbReference type="KEGG" id="loc:102691768"/>
    <dbReference type="CTD" id="11200"/>
    <dbReference type="eggNOG" id="KOG0615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00800000124190"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_47_1"/>
    <dbReference type="InParanoid" id="W5M806"/>
    <dbReference type="OMA" id="MLCAVQY"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003771">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22666">
        <property type="entry name" value="FHA_CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14084">
        <property type="entry name" value="STKc_Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000079">
        <property type="entry name" value="Checkpoint kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000354">
        <property type="entry name" value="Serine/threonine-protein kinase Chk2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000255">
        <property type="entry name" value="serine/threonine-protein kinase Chk2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="204"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="478"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="518" mass="58214" checksum="48305DC0EB643226" modified="2014-04-16" version="1">MSEEPGPSDTQPQSQSQSQSQSQSQSQSQSSSSSSSAPQSSSQSSSSSGSGTLSSVDTIPVRDLSSIPEEAEDPEEPPPQPWGRLLPMEKGFAMHNCIKDEYWFGRDRRCDYSFNNALIRKSEKYASYSKKHFRIFRESGIPYIEDHSGNGTYLDGQYMGQGKKLPLANNAELALSVPQNKVFVFIDLTVDEQSNLPKEFKDKYLISRKIGSGVCGEVKLAFERSTCKKVAVKTINKKDFSSTDRTNPSAEREIEILKKVDHPCLIKTVDFFQTEETYYIVLELMEGGELFDRVRSRSGLKESTAKLYFYQMLLAVEYLHNNGIIHRDLKPENVLLSSNSDVCLIKITDFNQSKILEEASLMKTLCGTPTYLAPEVFTHAETVGYSRAVDCWSLGVLLFVCLSGYPPFHTHQGINLSIKEQITQGLYSFLPSIWNKISEQAKDLVKKLLVVDPEKRLSVQQALQHPWLQDEDMRATAKKLMYPESVTGEDQPSSSRKRPLQENVDEQPSKRMSSGPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8M8</accession>
    <name>W5M8M8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:ch73-264p11.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8M8"/>
    <dbReference type="FunCoup" id="W5M8M8">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004736"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004744.1">
        <property type="protein sequence ID" value="ENSLOCP00000004736.1"/>
        <property type="gene ID" value="ENSLOCG00000003966.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682628"/>
    <dbReference type="KEGG" id="loc:102682628"/>
    <dbReference type="eggNOG" id="KOG0565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156202"/>
    <dbReference type="HOGENOM" id="CLU_125532_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8M8"/>
    <dbReference type="OMA" id="IQAAMCL"/>
    <dbReference type="OrthoDB" id="8815311at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003966">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051:SF1">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-RELATED PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="155" mass="17658" checksum="6FB8F806F157FAE2" modified="2014-04-16" version="1">MMMPQFDLQRCSAAGMQVYGAMDCPVYHGSISRQTCEELLSKKGKDGAFLIRDSETISGALCLCVFKKKVVYTYRILTNHAGYYTLQASPGVEEKFFKTLQDLIKNYKRSGQGLAARLQHPVKRKQQQPIYKNVLYDTPDYEDIPDKDYVDVLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9B8</accession>
    <name>W5M9B8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger and BTB domain-containing protein 44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642322.1">
        <property type="nucleotide sequence ID" value="XM_006642259.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9B8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004977"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004985.1">
        <property type="protein sequence ID" value="ENSLOCP00000004977.1"/>
        <property type="gene ID" value="ENSLOCG00000004162.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695308"/>
    <dbReference type="KEGG" id="loc:102695308"/>
    <dbReference type="CTD" id="29068"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153306"/>
    <dbReference type="HOGENOM" id="CLU_034849_1_0_1"/>
    <dbReference type="InParanoid" id="W5M9B8"/>
    <dbReference type="OMA" id="VHMPVIS"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004162">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18228">
        <property type="entry name" value="BTB_POZ_ZBTB44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000266">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 44 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000278">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 44 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF5">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 44 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="451"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="483"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="294"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="568"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="68722" checksum="F9E555EED4FF6C30" modified="2014-04-16" version="1">MGVKTFTHSSPTHSQEMLEKLNTLRNEGHFCDVTIRVQDKLFMAHKVVLACCSEFFRSKLVGKPDEEDKFVLDLHHVTVSGFTPLLEYAYTSTLSINTENIIDVLAAASYMQMFTVASTCSEFMKSSILWNANNTPQEKPQESAPENTTNCTLTPQDGSLSPVSSECSVMERTIPVCRESRRKRKSFIVMSPESPLKCSAQVTSPQVPNPSPSFPENQTQPIDSSLAFPWTFPFGIDRRFQSEKPKLPESPHSLEQPGPSDVTRRLADYLTCESSKVTSPLAAEEDVRVKVERLSDEEVHEEVSQPVSASQSSLSDQQTVPGSEQVQEDLLISPQSSSIGSIDEGVTEGLPSLQGPSNTSSHAEDDERLENVQYPYHLYISPSARPSTNGPDRPFQCPTCGVRFTRIQNLKQHMLIHSGIKPFQCDRCGKKFTRAYSLKMHRLKHEGKRCFRCQICSATFTSFGEYKHHMRVSRHIIRKPRIYECKTCGAMFTNSGNLIVHLRSLNHEASELANYFQSSDFLVPDYLNQVQEEALGQYELPEHSFEGSSSVQMPVISQVSSTQNCESTFPLAPLGGLTDKEEEEGVAEEPKTNASSSPEDPDEDPQKKGLAAMTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9E3</accession>
    <name>W5M9E3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Paired box 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9E3"/>
    <dbReference type="FunCoup" id="W5M9E3">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005002"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005010.1">
        <property type="protein sequence ID" value="ENSLOCP00000005002.1"/>
        <property type="gene ID" value="ENSLOCG00000004189.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0849">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156504"/>
    <dbReference type="HOGENOM" id="CLU_019281_8_0_1"/>
    <dbReference type="InParanoid" id="W5M9E3"/>
    <dbReference type="OMA" id="PDIACFQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004189">
        <property type="expression patterns" value="Expressed in embryo and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000080">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000035">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000031">
        <property type="entry name" value="Paired box protein Pax-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022106">
        <property type="entry name" value="Pax7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF17">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12360">
        <property type="entry name" value="Pax7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="15">
        <location>
            <begin position="34"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="221"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="223"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="161"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="53730" checksum="611015F1931FAC0D" modified="2014-04-16" version="1">MTAIAGAVPRMMRPSPGQNYPRSGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPKQVTTPDVEKKIEEYKRDNPGMFSWEIRDKLLKDGICDRNTVPSVSSISRIMRSKFGGKGEDEDDDDEIERKEFEENEKKTKHSIDGILGDRCKAASHSDEGSEVESEPDLPLKRKQRRSRTTFTAEQLEELERAFERTHYPDIYTREELAQRAKLTEARVQVWFSNRRARWRKQAGANQLMAFNHLIPGGFPPATMPTLPPYQLSDSPYPPTSISQAVSEQSSTVHRPQPLPPTSVHQGSLNPAPESSSAYCLPSSRHGFSGYTDSFVTPSGPSNPMNPTISNGLSPQVMGLLNPGGVPHQPQSDYAISPLTGGLEPSTAMSASCSQRLDHMKTLDSLPSSQSYCPPTYSSSGYSVDPVASYQYGQYGQSAFHYLKPDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9K5</accession>
    <name>W5M9K5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RAD17 checkpoint clamp loader component</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9K5"/>
    <dbReference type="FunCoup" id="W5M9K5">
        <property type="interactions" value="1069"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005064"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005072.1">
        <property type="protein sequence ID" value="ENSLOCP00000005064.1"/>
        <property type="gene ID" value="ENSLOCG00000004231.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039046"/>
    <dbReference type="HOGENOM" id="CLU_018598_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9K5"/>
    <dbReference type="OMA" id="YNCLKMA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004231">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001661">
        <property type="entry name" value="RAD17 checkpoint clamp loader component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018324">
        <property type="entry name" value="Rad17/Rad24_fun/met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00602">
        <property type="entry name" value="rad24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="39"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="516"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="584" mass="64238" checksum="6FECCF55597291EC" modified="2014-04-16" version="1">IDVHIPQKTSGELAVHKKKTEEVENWLKSHVGHKHLKEGGSILLMTGPSGCGKTATIQVLAKEQGIQVQEWINPTTSSHYGTEDFKQAFDPKSSFNSFYGSSQSAVFQEFLLRANKYSCLQMVGDDTDAGKKVILVEDLPNQFYRQPSLLHDILRRFVRTGRCPLIFIISDSLSGDGNARLLFPKDIQDELNICNISFNPVAPTSLLKVLSRIVSQEASRSGGKIRVPDKACLELLCSGSSGDIRSAINSLQFSSLADSTLEGSLWPLKKGKSSTLANRGGSKGRSKGKSSKLPGKQDAEQAVGGKDASLFLFRALGKILHCKREPHVEPEVPGLPAHLTEHQRDALLVDPEMVVEKSHMSGELFNLYLQQNYVEFFTEIEDMARASEYLSDADFLSAEWSSQSTMREYGSSVATRGMIHSNRSRAFASSQTGVGFKPLHKPHWLLISKKYRENCQAAQALFVSFCLTPVSLQTELLPYLAQLTNPMRNQAQIAFIQDVGKLPLKKYPGRLRLEALTDKDPGTLDLDSEEEEPTAASSSACEPPDDGPALSPGWSDLMGSQPEPITAQDPLEKEEELVIEDSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA81</accession>
    <name>W5MA81_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dopamine receptor D2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000005290.1"/>
        <property type="gene ID" value="ENSLOCG00000004412.1"/>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_009579_11_1_1"/>
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        <property type="component" value="Linkage group LG26"/>
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        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="64"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="459" mass="51774" checksum="5486D499FA1987AE" modified="2014-04-16" version="1">LIAGPSHSVLMESFLDNETYYGNETDVANGTERVQRHQYNYYAMLLTLLIFVIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVVWVLSFAISCPLLFGLNNTATRDDALCVIANPAFVVYSSIVSFYVPFIITLLVYVQIYVVLRKRRKRVNTKRSSHGMEADVHTTLKDKCTHPEDVKLCTMIVKSNGSFPVNKKKVILIQEAVNHPDDMEMDMMTSTSPPEKKKHKPAAPQAHLSMPATSNQCQSMALPSPADSPVKAEKNGHTRELPKAAKAFEIQAMPNGKTRTSVKTLSKRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNTHCTKCRVPLEMYNAFTWLGYVNSAVNPIIYTTFNIEFRKAFIKILHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA99</accession>
    <name>W5MA99_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000005308"/>
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        <property type="gene ID" value="ENSLOCG00000004447.1"/>
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        <property type="component" value="Linkage group LG23"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007198">
        <property type="term" value="P:adenylate cyclase-inhibiting serotonin receptor signaling pathway"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000232">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF1">
        <property type="entry name" value="ADENOSINE RECEPTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="74"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="41779" checksum="BC08635B0A02A9EF" modified="2014-04-16" version="1">LSAVSALLSNLTGPWGTRGLAGPTWGPNSTGAAPAGPGDGDMRAPLCTFCCCGLLNRTLAVVFMVSLAFSIVIGNVVTITVFLQTRQFRTPQGYLKVSLALADMMVGLLVVPFSVYTEITLMVTSTPPAWYQGDSVAARGGLGSSWQPCKLIGPVFAGCTFVSISTIFLMTVERCVAILKPLHKDSILTHRRILLLIALSWVGSFLLALAPLILTNGFTLEYNECSRMCNYAPLWDGKPPPPKGNVLLLFPAFDLTLLGVTLVVNVMSFTSIRRYSQKRKLLAEGGLGGDSSTGGCSQRPSFSDIKAAKTIGILTFAFTASFFPIAVFVLGNVVGYQWCNFSFFAFWILTSNSCCNVIIYSVRDQRFRKGVAVLFRKEPVPPHGEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC04</accession>
    <name>W5MC04_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">25/26-hydroxycholesterol 7alpha-hydroxylase</fullName>
            <ecNumber evidence="18">1.14.14.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Oxysterol 7-alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 20 21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 20">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20 23">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.29" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01004383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC04"/>
    <dbReference type="FunCoup" id="W5MC04">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005913"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005921.1">
        <property type="protein sequence ID" value="ENSLOCP00000005913.1"/>
        <property type="gene ID" value="ENSLOCG00000004895.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153141"/>
    <dbReference type="HOGENOM" id="CLU_018012_1_3_1"/>
    <dbReference type="InParanoid" id="W5MC04"/>
    <dbReference type="OMA" id="WSEVVQM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004895">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033783">
        <property type="term" value="F:25-hydroxycholesterol 7-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20632">
        <property type="entry name" value="CYP7B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000065">
        <property type="entry name" value="Cytochrome P450 family 7 subfamily B member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF0">
        <property type="entry name" value="CYTOCHROME P450 7B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="9 20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 20 21" id="KW-0349">Heme</keyword>
    <keyword evidence="12 20 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0492">Microsome</keyword>
    <keyword evidence="13 23" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11 23" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <feature type="binding site" evidence="22">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="500" mass="57836" checksum="00E6D33655BD2A8E" modified="2014-04-16" version="1">IIINVLSFPCFIFGEYVYLWKSSSITLNRQNGEPPLITGWIPYVGKTLEFCKNPYKFLNAQKEKYGDIFTVYVAGKYITFIMDPTLYPSVIKHRKQLDFRDFSDQVAPVTFGYPPIRNPKFPGIDEHIHRGYQLLQGDNLSPLTENMMKNLLFLFRQDYLSKEPQWRTEKMYSFCSKIMFEATFMTVFGKSGHTSRHADMASFREKFDKFDAMFPLLIAGIPIKFLGATKKIREDLINIFLPERMAHWKESSEFVKVRAELFEQYELLGSVDKAAHHFSILWASVGNTIPATFWAMYYLVRHPQALAAVRDEILCALQLTGEESGRGQDIILTREQLDSLLYLGSAINESLRLSSASMNIRIAQEDFFLELDKDWSVGVRKGDIIALYPQSMHMDPEIFEDPEIYKFDRFVEGGKEKTNFYKHGQKLKYYWMPFGSGATKCPGRYLAVNEIKQFLSLLLLFFEIEEVPGEAQVKLDNSRAGLGILLPDSDIHFCYRVRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCE0</accession>
    <name>W5MCE0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymocyte nuclear protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCE0"/>
    <dbReference type="FunCoup" id="W5MCE0">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006049"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006057.1">
        <property type="protein sequence ID" value="ENSLOCP00000006049.1"/>
        <property type="gene ID" value="ENSLOCG00000005023.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3383">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013297"/>
    <dbReference type="HOGENOM" id="CLU_041799_2_0_1"/>
    <dbReference type="InParanoid" id="W5MCE0"/>
    <dbReference type="OMA" id="DVQFIRM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005023">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21133">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000003">
        <property type="entry name" value="Thymocyte nuclear protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052181">
        <property type="entry name" value="5hmC_binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047197">
        <property type="entry name" value="THYN1-like_EVE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087:SF7">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="7">
        <location>
            <begin position="73"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="242" mass="27427" checksum="48B90247292A5663" modified="2014-04-16" version="1">QRMPPKKRTRGTTAEHEEPAAKLAKKDDDEKDSSGKGAKGAPRRRGKPEAGNSGMQEDKGAAAAPAGKEVYCHWLMKSEPESRIEKGVDVKFGIEDLKALPKQTGCWDGVRNYQARNFMKDMKVGHEAFFYHSNCKEPGIAGLMKIVKEAYVDHTQFDKKDAHYDPSSKKDNPKWYMVDVQFDRMTKRFIPLSELKKYHQQHKSNGGVLKGLALFTRARLSVQPLSKEEFDFVLSLEDKEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCR6</accession>
    <name>W5MCR6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain-containing protein 46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">BTB-ZF protein expressed in effector lymphocytes</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">BTB/POZ domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a transcriptional repressor for PRDM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01035851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220476.1">
        <property type="nucleotide sequence ID" value="XM_015364990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCR6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006175"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006183.1">
        <property type="protein sequence ID" value="ENSLOCP00000006175.1"/>
        <property type="gene ID" value="ENSLOCG00000005130.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683619"/>
    <dbReference type="CTD" id="140685"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158060"/>
    <dbReference type="HOGENOM" id="CLU_022356_2_0_1"/>
    <dbReference type="InParanoid" id="W5MCR6"/>
    <dbReference type="OMA" id="WPADSGM"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005130">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18230">
        <property type="entry name" value="BTB_POZ_ZBTB46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001046">
        <property type="entry name" value="Zinc finger and BTB domain containing 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000045">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000379">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF21">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="18">
        <location>
            <begin position="31"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="167"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="502"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="511"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="597" mass="65744" checksum="FCD6A2768B9F35DC" modified="2014-04-16" version="1">MNNRKEDMEITSHYRQLLRELNEQRQHGILCDVCIIVEGKIFKAHKNVLLGSSRYFKTLYCQVKKTSDQATITHLDIVTAQGFKTILDFMYSAHLALTSKNVIEVMSAASYLQMTDIVQACHNFIKAALDISIRSELSDELADFDMSAPPGGSSSAEALASMMSGRSTSPWLARKTSPANSSGDSAIASCHEGGSTYGKEDQEPKSDSHDDISSQSLWSSDFRSVQVKEEQVSPSNFCKDGANVGQNALGEQANGDSWQPSGRRKNRKNKDTVRHITQQVEDDSRAGSPLPSILSAWNYNNQDISGPDVAEPSSSDSRGDRLDFFVKQEESLLGDSSYLSQSLGSEKEDVSSQAASVANLRAALMSKNSLLSLRAEMLGEDNPLLFDYLPKGGHSLSLNEFTVIRKKFKCPYCSFSAMHQCILKRHMRSHTGERPYPCEICGKKFTRREHMKRHTLVHSKDKKYVCKVCSRVFMSAASVGIKHGSRRHGVCADCSGRGMSALLDQNGGEGSPEDELYPGENQFADDPDDLKADGDEEMGDADAEGEGEGEGEEDGKWKDDSGMSHQDVILDDEKEESDSAQEPENQGGSDKDFAWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCX3</accession>
    <name>W5MCX3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Adhesion G protein-coupled receptor D2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCX3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006232"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006240.1">
        <property type="protein sequence ID" value="ENSLOCP00000006232.1"/>
        <property type="gene ID" value="ENSLOCG00000005172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161534"/>
    <dbReference type="HOGENOM" id="CLU_015074_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCX3"/>
    <dbReference type="OMA" id="SCEQQFG"/>
    <dbReference type="Proteomes" id="UP000018468">
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    <sequence length="1070" mass="119590" checksum="9B085470F111C4DB" modified="2014-04-16" version="1">MIGDRGKSSFLVYFVLVSSITGKIRSWECENWRNSQVFGSSECFYETPEDVFQFVNISMDFWQANGFCQHHFSKLLSDEMDSDVEYTSKLLQAAQMKHPIWIVDVYHYNWRFPPFLGKYVSIPALNFKEESHAMYARVDIEFPPLPAVTVCARVQWHSANSQVSTVFSYAALQLTNEFQLRGQQDKDGNIILALIVHGNHKPYKASFRNDGKWHHLCVTWQKYNGNWAIHVDGKRRDSGNGTETSRNIYGSGIFIIGHDQDSFGGNFTEPFVGNITALGIWNKTLDEHQINDLSVCSHISGDLMFMWNLSQMTVKPAPQLSLTKLWTLCAADPKQIPSEECRTISVDPSQQLSLYKYVPCQRTLPFVCKHNKDSFLKMKEIEEALEGKPSHFMHCLTQLSNDTNTSFSVAVRDLSVPEVHDILKFSQQALVDVGTPLEPADMVSLVQFLSQAAAVKIRGNESHDAIRNMSSFFITVADTMFREENINKWMAVKEIVNGPMTVVHSIDRMVANLNPLLMAKKSRIVFDSTNIKLEVRQKGLGSGADGSDFHGPTPENETKVDFITVPEDKIQQLYQHGIQNITLINTWYGSLLPLFGIEDNITQFSTVTDGSQNNGPRHVGTVLGSSVISATVLGDNRPVNTAVNYELRHRLENLHGAEYEPICAFWDFHLKPEKGGSWSTEGCFITSLGYESTTCFCNHTTNFALLLQIYEVQRTPQDENALRILTFVGCGVSLCGLTMTFILFIAVGVPNSDRTTVHKNLIFALGVAQLLLMFSDLASTNKGLCTAVTALLHLFFLGSFSWMLVEGLLLWSKVVSVNISEDRRMKLYYAIGWGLPVVIVGITLATSFNKYVADNYCWLNAQTDVIWAFVGPVIFVLSVNTVVLFRVVMVTVASARRRSKMLTPSTASQIHALDLTWTATKPVIILLPVLGLTWLCGILVHLSVILAYIFIAMNAFQGLYIFLVYAVYNSEVRNAIRRMKEKKKALSFTNCSQPISFLTSQRATGSWDNSKPHPSASENSESSAGQTGSTKKSLVIQNESFGKDSFVSFSLKPASGNQGVQLTAFKPSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEI8</accession>
    <name>W5MEI8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome P450 2J2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006797"/>
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        <property type="protein sequence ID" value="ENSLOCP00000006797.1"/>
        <property type="gene ID" value="ENSLOCG00000005628.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182879"/>
    <dbReference type="HOGENOM" id="CLU_001570_22_0_1"/>
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        <property type="component" value="Linkage group LG10"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in liver and 8 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:iron ion binding"/>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
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        <property type="term" value="P:organic acid metabolic process"/>
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        <property type="term" value="P:xenobiotic metabolic process"/>
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        <property type="entry name" value="CYP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cytochrome P450 2D15 isoform X1"/>
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        <property type="entry name" value="Cyt_P450_E_grp-I"/>
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        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2D-like"/>
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        <property type="entry name" value="Cyt_P450_sf"/>
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        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME P450 2J2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01686">
        <property type="entry name" value="EP450ICYP2D"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="501" mass="57145" checksum="919BD29BCF22A490" modified="2014-04-16" version="1">QEYLISMLHYILEGLDAQSILLFLAVFLLIIDIVKNKNPKNFPPGPWGLPFVGNVFNIDTKHPHIYMGKLAENYGDVFSLRLGREKIVFVMGYQLVKDALVNQGDAFADRPCGPFADAVYGGLGLFLSNGYRWKKQRRFALSTLRDFGLGRKTLESVISDESRFLQEAIEEEQGKPFNPHLLLNCGISNIICSLVFGHRFHYSNNSFQRMLYMMSVIMHLEGSIWAQLYDAFPSIMKCLPGPHKQLFSYHREVKAFIREQIKKHKEDWDPSTPRDYIDSYLTQIENSAKDAAAGFHEENLVLCSLDLFIAGTETTTTSLLWALLYMIKYPDIQKKVQAEIDRVIGKERQPSMADRVYLPYTDAVIHETQRHGNTVPLNAPRMTNKDTTLGGHFLPKGTTIIANLTSVLFDKTEWQTPNTFNPGHFLDSEGKFLRREAFIPFSAGGRMCLGEQLARMELFLFFTFLLQKFTFTSTDGKQPSMEYKLGVVRNPLPFSICAVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MER8</accession>
    <name>W5MER8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MER8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006877"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006885.1">
        <property type="protein sequence ID" value="ENSLOCP00000006877.1"/>
        <property type="gene ID" value="ENSLOCG00000005698.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159163"/>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="InParanoid" id="W5MER8"/>
    <dbReference type="OMA" id="CKENYAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005698">
        <property type="expression patterns" value="Expressed in embryo and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="175" mass="19048" checksum="57A986B852215E7C" modified="2014-04-16" version="1">MSGTQYNAQKIHYHTGFKQSTNDYDIALLRTATPISFTDTVRPVCLPAYGQIFPSGETCWISGWGFTAEGGPISLVLREAPVLLINNKVCSTMDIYGINITPRMVCAGYMDGGIDSCQGDSGGPLVCLSDGSWKLVGVVSWGEGCGRPNRPGVYTNVTELLDWLYYHIQADKDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF09</accession>
    <name>W5MF09_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF09"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006976.1">
        <property type="protein sequence ID" value="ENSLOCP00000006968.1"/>
        <property type="gene ID" value="ENSLOCG00000005769.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_217780_1_0_1"/>
    <dbReference type="InParanoid" id="W5MF09"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005769">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866129" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="43" mass="4407" checksum="C96747875DAFD106" modified="2014-04-16" version="1">MPSVCVCVCVCVCVCVCVCVCVCVCVCVCVCVCVCVCVCPVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF52</accession>
    <name>W5MF52_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RB1-inducible coiled-coil protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">FAK family kinase-interacting protein of 200 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy. Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis. Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway through induction of RB1 expression. Plays a crucial role in muscular differentiation. Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000007019.1">
        <property type="protein sequence ID" value="ENSLOCP00000007011.1"/>
        <property type="gene ID" value="ENSLOCG00000005780.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015871"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005780">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17060">
        <property type="entry name" value="Ubl_RB1CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000049">
        <property type="entry name" value="RB1-inducible coiled-coil protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="19">
        <location>
            <begin position="1488"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="216"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="637"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="859"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="954"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1169"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1298"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1463"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1603" mass="183362" checksum="436FCDCF270241D2" modified="2014-04-16" version="1">MKLYVFLVNNGNTLTFNTELAVQTVADLKNAIQSRYRIAPQNQVLVVNGGECMVADRRVCSYSAGTDTNPIFLFSKEMIFCDRPPTIPKTTFSVENDMDAKVEESLLMPAVFHTVASRTQLAVEMFEIAKKICSYCEQQVHDEHLQHQGWAAIMANLDDCTLSHQKLLFKFETAYISYQQSIEDIKQKLNNLGTAVSVMAKIPLLECLTRHSYRESLERSSSPAEKEGTEEETEEEKSSDSVLCPGQPRTSNKSSASFSISVENSPQDSIAQEDHETTKSSSPRAELQDADCQEKSDQPLFNVSLLDWINVQDRPNDVESVVRKCFDSINRLDPRIIQPFLAECRDTISKLDNQNMKAIKGLEDRLYALDQMIASCKKLVNEQKELAQGFLANQKRAENLKDTSVLPDLCLSHANQLMIMLTNHRKLLDIKQKCTTAKQELTNNLQVRLKWCCYVMLHADQDGEKLQALLRLLTELLERVRVVEALGTVPQMYCLAVVEVVRRKIFIKHYREWANALVKDGKQLYEAEKLKRETFGKLFRKSFLRNRLFRGLDSWPPSSFCTRKPRKFDFELPDISLNDLQFLKSCCPAEVQPFLRVPTLCDFEPLHQHVQILHQLVKAAQSVDEMSQTITDLLSEQKASNSQAAQRSTTIPRSESATGTISTSSKTPTSLTLQAPLCQPLNLPAPLEDLSPDSIDAHTFDFETIGHPNMDPVLKQESLDLDSLAESPESDFMSAVNEFVIEENPASPTAISDPTSPEMMVESLYSSVINAIDSKRIQDTTTLEKENSRIAMLKVCIEKYRFAAEESQCNLRSIKEDLCHFRGLVQKEQRDFSYALKSVSAEIQNIIVSIRYSLEEDLKQQHQQELQLLKTDHEKEIQNLKDVSEVNQNILKDLKKALLGLEETIQHKENEISLLKEEKDRFVKSESCQKQKLCDLEQNILAQNNEIQSLLQSRESLADQLEHLHFEIERSQQKMKLELESLEQSHLKDLAERLQAKHGEDLEALKNEHQVERDALTKENMAKLDELLEVHRTELRARESHLKELELRISELSDVRCKLEVEMALKEAETEEIKLLYEESKSHQEETINSRVEMETKALRKEISDLKQQLQTKNDEYEVGIAELRTLMRIEKDHCISELVDRHEEETTLLHSELSSVRQKALDTEKALAEQVQQIQSDLENHLAAHKKETEENERAFQEQQQQLKTIICQLQAENELLSNSLAQVKQTVIQGMDKEKEEAVKAALSEATKEFEHRKGLIEEQLLERVKQLENQLKEKGFGARHSEAISEKPVLDTDLQERLQEESAALRTQLEDLEKRKNEELQTLKTSLIAEQQTNFNTVLTREKTKKEQIINELSDKLKKITEQQERDKDLIETLSKDRAEMLQEKKKLEEELNRLRSSALVSSAFFSANQPDAQAAGAFSTEAPADSERLASVAAIGEDGRIDPAVEASMMAVHENITMLSEEKQRILLLERTLHMKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVGPTLYFLHSESLAALDLKPASGTPRRPWVLGKVMEKEYCQAKKALNRFKVPLGTKFYRVKAVPWNKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGY7</accession>
    <name>W5MGY7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GDP dissociation inhibitor (GDI) gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637221.1">
        <property type="nucleotide sequence ID" value="XM_006637158.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGY7"/>
    <dbReference type="FunCoup" id="W5MGY7">
        <property type="interactions" value="1652"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007646"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007655.1">
        <property type="protein sequence ID" value="ENSLOCP00000007646.1"/>
        <property type="gene ID" value="ENSLOCG00000006313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686078"/>
    <dbReference type="KEGG" id="loc:102686078"/>
    <dbReference type="CTD" id="398"/>
    <dbReference type="eggNOG" id="KOG3205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006233"/>
    <dbReference type="InParanoid" id="W5MGY7"/>
    <dbReference type="OMA" id="YKPTAAK"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006313">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000004">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF8">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="25599" checksum="0DD2530987EEBD74" modified="2014-04-16" version="1">MLGLDVCGLGGQVLELLWLTVCYRDIMADKEAEKHIADEEEETELNYQPPAQKSLQEIQELDKDDESLNKYKQALLGSGPVVADPTVPNVEVTRLTLVCGQAPGPITMDLTGDLEALKKQNFVLKEGVDYRVKIHFKVNRDIVSGLKYVHHTYRKGLRVDKAVYMVGSYGPRTEEHEYMTPVEEAPKGMLVRGTYHIKSYFTDDDKTDHLSWEWNLNIKKEWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHJ0</accession>
    <name>W5MHJ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear factor, erythroid 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHJ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007849"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007859.1">
        <property type="protein sequence ID" value="ENSLOCP00000007849.1"/>
        <property type="gene ID" value="ENSLOCG00000006498.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="HOGENOM" id="CLU_618156_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHJ0"/>
    <dbReference type="OMA" id="LPLRRNC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006498">
        <property type="expression patterns" value="Expressed in mesonephros and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071600">
        <property type="term" value="P:otic vesicle morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010725">
        <property type="term" value="P:regulation of primitive erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902882">
        <property type="term" value="P:regulation of response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048798">
        <property type="term" value="P:swim bladder inflation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF26">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-E2 45 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="367"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="473" mass="50999" checksum="7D01A9C2EB6DB93C" modified="2014-04-16" version="1">MCAAANCILPLRRNCEGLAHPGRLHGGVPMSTAVPPARPHRPLPEAEMDFTWQELMAITELQEFDVPGESPFEQAAYQPVCPQLTPVGFGLSQPPLEPPLPSCGPGPGDAFESPYTEAMPPGAPACSLQRLGSNIETLYGHAGSRLLPNPHSHSHSHSHSLQPPLMSLLEHMSLGGAGEAGAPPFMGAGQGRGPPGAGLGPIKHPPPSDDPESDSGLSLGSSPPLASPGNAAGGAPPYGGGAAEAGLGYSDGEPPLEMEGMAEPGRMRPDFLYPMDYQHPMNHYPYPVPQPSYSLPPPPLHPHQQHQQHQQLPSDLQPHPIAPAALHGLVPGALKIPFALEKIVNLPVDDFNELLSRYQLTDAQLALVRDIRRRGKNKVAAQNCRKRKLENIVHLEAELGQLRARREQLARERAEYQRGLALAKRRLSELYADVFARLRDEEGRPYSLDDYSLQQTSDGSVFLVPRKPGEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJL8</accession>
    <name>W5MJL8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJL8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008577"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008587.1">
        <property type="protein sequence ID" value="ENSLOCP00000008577.1"/>
        <property type="gene ID" value="ENSLOCG00000007084.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164382"/>
    <dbReference type="HOGENOM" id="CLU_064620_4_0_1"/>
    <dbReference type="InParanoid" id="W5MJL8"/>
    <dbReference type="OMA" id="VRFEYRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007084">
        <property type="expression patterns" value="Expressed in muscle tissue and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="116" mass="12520" checksum="74ADA60B6FB6FA98" modified="2014-04-16" version="1">AGPQGAPGAPGEEGGAPGSGSPPAPPNLSSNRRMQQTQAQVEEVREIVRVNVDKVLERDQKLSELDDRADALQAGASLFESSAAKLKRKYWWKNCKVGLWATALIQYLSVVFAQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML45</accession>
    <name>W5ML45_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonucleoprotein PTB-binding 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Protein raver-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PTBP1, RAVER2, VCL and ACTN1. Part of a complex containing RAVER1, VCL and ACTN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML45"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009104"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009115.1">
        <property type="protein sequence ID" value="ENSLOCP00000009104.1"/>
        <property type="gene ID" value="ENSLOCG00000007500.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685734"/>
    <dbReference type="KEGG" id="loc:102685734"/>
    <dbReference type="CTD" id="125950"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160550"/>
    <dbReference type="InParanoid" id="W5ML45"/>
    <dbReference type="OMA" id="KENFMAG"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007500">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12663">
        <property type="entry name" value="RRM1_RAVER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12665">
        <property type="entry name" value="RRM2_RAVER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12667">
        <property type="entry name" value="RRM3_RAVER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000100">
        <property type="entry name" value="Putative ribonucleoprotein PTB-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000116">
        <property type="entry name" value="Putative ribonucleoprotein PTB-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000125">
        <property type="entry name" value="Putative ribonucleoprotein PTB-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034633">
        <property type="entry name" value="RAVER1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034635">
        <property type="entry name" value="RAVER1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="91"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="164"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="253"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="810" mass="87311" checksum="2A63BFD2AAF210A0" modified="2014-04-16" version="1">MAAAVSVGAAAGGDENLNLGPILKSPSSYDCDPAAENEDWFPGEGGGEPGEEENTAGDEGPRAAEEELSVLSPEEIAGRLDRTRREFYNRRKILIKNLPPDITNQEVHDLLSDYDLKYCFVDKYKGTAFVTLLNGEQAKSAIKEYHQYSLRDRDISVQLQPTDALLCIANLPLSFTQQQFEELVRPFGNLERCFLVYSESTGQSKGYGFVEYMKKDSAARAKSELLGKQLGTRMLYVHWTEVNSLTYPLLHSRCLCVDRLPRGLQDAEGLQEIFTQFHPPVFCQLAQGQDGNFRRFAVLEFETAEMAEMFQRSTDGLAVGKTRIRVSFCAPGPPGRSMLAALIAAQTMAMNRGKGLLPEPNAMQILTSLNNPATLKMLLTPLQQGRKHGLLGAAPAMPLLANPALTAALLQLVLQNQAQLQQNALLGSPLLLQNLMWAQLLHNKENQAGLSPGVLGENPLSVLPPQNILQQGMGILGDLPPGGVLPSVGLAPEVPPPGPIKPTTLPGGNMPLPHLLGGGSVDRESPTTVGGHFPQSAPSPALQGIVTPILGNVLGGIPQQPSAGLPADGGSLMPGLSLLGEPPKDLKIPRNPFLNIHSAFPSASMSGSGANSRPHPYRKKPAVLGNISNPRNMHSSHSDFSPSSSLQYQDSYLYGDYKPINQDSMSHMYQQLDALDPSALRGYNHHKQKSPYQSGYSERGAFNLSPVPSYHSGSPTAYFKSGMSAGIQQTHLNKAVGMPPVSNGNSLYGYGPSSSPPGNIMKTPVGGQKRVFSRLIPSPEPSPEGSYVGQHSQGLGGHYADSYLKRKRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML74</accession>
    <name>W5ML74_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Crumbs cell polarity complex component 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009133"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009144.1">
        <property type="protein sequence ID" value="ENSLOCP00000009133.1"/>
        <property type="gene ID" value="ENSLOCG00000007531.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155152"/>
    <dbReference type="InParanoid" id="W5ML74"/>
    <dbReference type="OMA" id="RDMFIML"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007531">
        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000348">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000081">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000130">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000055">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000282">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000252">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000575">
        <property type="entry name" value="Crumbs, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001021">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867925" evidence="17">
        <location>
            <begin position="28"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1390"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="195"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="348"/>
            <end position="404"/>
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        <location>
            <begin position="406"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="450"/>
            <end position="490"/>
        </location>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="673"/>
        </location>
    </feature>
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        <location>
            <begin position="675"/>
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        <location>
            <begin position="717"/>
            <end position="889"/>
        </location>
    </feature>
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        <location>
            <begin position="891"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="955"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1143"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1181"/>
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        </location>
    </feature>
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        <location>
            <begin position="1330"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1427"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1427"/>
            <end position="1436"/>
        </location>
    </feature>
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            <begin position="145"/>
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    </feature>
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    <feature type="disulfide bond" evidence="14">
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            <begin position="701"/>
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    <feature type="disulfide bond" evidence="14">
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            <begin position="1185"/>
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            <begin position="1206"/>
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        <location>
            <begin position="1244"/>
            <end position="1253"/>
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        <location>
            <begin position="1318"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1356"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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            <dbReference type="ARBA" id="ARBA00060989"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
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            <dbReference type="PROSITE" id="PS50026"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000009133.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="1454" mass="160647" checksum="E9D2A912A8AB16DD" modified="2014-04-16" version="1">MAFPKPSRLKLIGIICIFLTLFRTASAFPLEAFASCLSGPCQNDAVCEDTFRDHICYCPSALITPLNKNCDEANPLCHLSTCKNNTTCMKATEDSIDLICQCTPNDTREVCERSVQKCADSLCESGTHCQQRPVTDEFAGYRCICKDGYIGTHCETEVDECASSPCQNRAVCRDRVNGYSCYCVPGFQGKHCEIEVNECASQPCQNGATCLNKIGKYVCICMPGYTGTSCELEINECQSEPCFNRARCHDYQDGYSCTCTPGFQGDLCEINIDDCRSQPCQNGAQCVDEVNGYSCECSKTGFMGLHCEIPVPLCMSKPCLNNASCEEDHGNYKCFCWPGFSGTNCELDLKECDSDPCLSGGECIEFSWKELYGTIPELPVLFDYQHAAGFVCRCQQGFKGVLCQEDINECIMNPCQNGAKCENTVGSYVCHCLQQSLEGPLYGGQNCTDILMGCENHDCQNEATCIPFLKNEQHEYSCVCPNGYAGSKCEIPTTFSFEENGYFYIGTNLQKDSINITLSFKTVLPNTLLFYQGNEDTVIQLELVNGLLHFIIHKANELSSSLELPHNVTDGEWHTVEATLGGRMFGIVLSDRSCIRDCEQKVTLEILHYQHESAFWNRSIGGLKEEGKKPGTIDAIHSQPYFIGCLQDVYVDSQLVVPSSISNATAVNVIPGCSSKDRCVANPCQNRGRCINLWQNYHCECYRPYEGQNCSNEYVTARFGSDNSEGYALFTIDDSPGQNITISMFVRTRRYTGLLLVLTNSTSHYLRIWLENGKVNVRVNDFETLSGDRFISDGHLHLLSIKIEQNQMALYQSVQKLNYTSIHTVNVQLGDLVYVGGLADKRIPASFGGYFKGCIQDLRINTKRLEFYPIGIPLSSYRITSLVNVTRGCSGDNVCDTNPCQNGGVCYAMWDDFTCTCPPSTAGRTCEEVQWCELSPCPAEAVCQSRLKGFECICNVTFREDSSALSYKGNGKIFRNLTNITFSLRTRKHEAVILHAEKGPEFVTISIQDSHLLFELVSGNSFLTLSMKSKMPINDGQWHKVMFSMLTPKSESSEWVMVLDNTEEPSSTNVAAGNLNFLKEETNIFVGSLGPDMGGNLAGCLSTIEISSISLSYFGNTETHLPRYQQEQFIKTSAKPAILGCSGANVCAPSPCLNGGSCKDLFNDYNCTCPPGWTGLKCEINIDNCASNPCIHGNCTDRTLAYECKCDPGYTGRNCELEIDECKNHQCGNGATCLDGINRYFCLCPQNATGSQCDVKIEKVSWYLPETRMYPLPEATSVPVVRIYPMLPVSICRNSRRNYTCFNGGNCTDAGESSRCNCQSGFAGDWCEIDVDECKSNPCLNGGFCRNMLNSYQCICNVSYAGERCEVDTETATTPVFTTDSETPQNKPHLAAVLAPSLILGAITIGIVLVFTIIKIREKRQLEGSFNPRSLEQAGSQADKGSCPMKLPPVERLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLB6</accession>
    <name>W5MLB6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">complement subcomponent C1r</fullName>
            <ecNumber evidence="4">3.4.21.41</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).</text>
            <dbReference type="EC" id="3.4.21.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules. Within the C1 complex, C1R is a dimer of identical chains, each of which is activated by cleavage into two chains, heavy and light, connected by disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="27">The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.41" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01034756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLB6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009175"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009186.1">
        <property type="protein sequence ID" value="ENSLOCP00000009175.1"/>
        <property type="gene ID" value="ENSLOCG00000007540.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183084"/>
    <dbReference type="HOGENOM" id="CLU_006842_14_1_1"/>
    <dbReference type="InParanoid" id="W5MLB6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007540">
        <property type="expression patterns" value="Expressed in liver and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000059">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000016">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000006">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000012">
        <property type="entry name" value="mannan-binding lectin serine protease 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024175">
        <property type="entry name" value="Pept_S1A_C1r/C1S/mannan-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255:SF25">
        <property type="entry name" value="COMPLEMENT C1R SUBCOMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255">
        <property type="entry name" value="COMPLEMENT COMPONENT 1, S SUBCOMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001155">
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            <begin position="457"/>
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            <position position="75"/>
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            <position position="83"/>
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    <sequence length="708" mass="78347" checksum="0960E44F99442941" modified="2014-04-16" version="1">YNVLPVLFYDSCRIRLLCVSLCLAVALSGSSPVLYGEVQLPGYPNPYPASLNEVWDLAVPQGFALQLTFRYLDIEPSQDCYYDSLTVMSGKKVLGRFCGQNSADENHPGNSPILSPSNRLRLLFQSDSSSPGPHQHIGFKAFYQAIDVDECASPVQEGEEPLCQHYCHNILGGYLCSCRHGYELQPNNRTCQLQCGGGVFTEPRGLISSPGYPNLSPPGLSCRYTIRVEPGFHITLHFLGLFDIEHQLSQCPHHWLKVSIPGHADLLLCGSEAPAPIVSSSNEVRLEYHTDQRGDSRGWRLEYTTERVQCPSPRSISHGRVTPQFLKYQFRDYIRVKCDTGYKLMMGEKEIGGYFSMCKSDGQWHLPLPECRIIDCGTPSNLLNGGVQFLSGSLNQYQSVIHYHCNEPYYVLERGAVVNYTCAADRKWTDSNSNYIIPTCFPVCGKPSASPSLTQRILGGQDAEEGAFPWQVFLNTFYGRAGAALIADRWILTAAHNVHSKKHSLQSLETIVESLEVYVGDNDVENILKNKLSVEAVFPHPGFSQSSTSFDNDLALIKLKEPVTVNRHIMPLCLPPEGTPYSPGSVGYVSGWGVTEEDTISNRLKFIRLPVVEQEKCRSSIESQRGRQRDIPTLTENMFCAGLPEGGKDTCQGDSGGAFTLQKGGSGGAFYAAGIVSWGIKCGEKGTYGVYTKVGNYLSWIKETMEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMF5</accession>
    <name>W5MMF5_LEPOC</name>
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            <fullName evidence="4">LUC7-like (S. cerevisiae)</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000009564.1"/>
        <property type="gene ID" value="ENSLOCG00000007877.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Linkage group LG13"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="94"/>
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            <begin position="232"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="373" mass="44372" checksum="0218FA066B243D33" modified="2014-04-16" version="1">MSAQAQMRALLDQLMGTARDGDETRQRVKFTDERVCKSHLLNCCPHDILAGTRMDLGECSKIHDLALRADYEIASKDRDLFFELDAVDHLESFIADCDRRTELAKKRLAETQEEISAEVAAKAEKVHELNEEIGKLLAKAEQLGAEGNVDEAQKVLQEVEKVRSRKREAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLEQLKKTVAEKQEKRNQERLKRREEREREERMRKRTRSRSREHRRSRSRERRRRRSRSSSRERKRSRSRSRDRDRRRRHRSRSRSRGHRRSRERAAKHKSSRDRDRSSRDRSRDRDRDRKEKSSSERRQESMNGRSDSRRSEEREAGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN98</accession>
    <name>W5MN98_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 131</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN98"/>
    <dbReference type="FunCoup" id="W5MN98">
        <property type="interactions" value="1369"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009857"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009868.1">
        <property type="protein sequence ID" value="ENSLOCP00000009857.1"/>
        <property type="gene ID" value="ENSLOCG00000008117.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154668"/>
    <dbReference type="HOGENOM" id="CLU_031851_0_0_1"/>
    <dbReference type="InParanoid" id="W5MN98"/>
    <dbReference type="OMA" id="PTEHTQY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008117">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000498">
        <property type="entry name" value="Putative zinc finger protein 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000552">
        <property type="entry name" value="Zinc finger protein 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000041">
        <property type="entry name" value="Zinc finger protein 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF55">
        <property type="entry name" value="ZINC FINGER PROTEIN 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="34"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="357"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="550"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="575" mass="65663" checksum="98537FDC957FEEB8" modified="2014-04-16" version="1">MSEEEVVEYGSEFPDHYKVMLDRLNEQRQLDQFTDITLIVDEHRFRNKHKIVLWLCTSFVFKKYLDVLCLPSCPLTGVSNLAFRHLIEFTYTAKLAVNGHEEVNDVWKAAEYLQMQEAIKALNSRTNSSPPYTEVQESASRNKAKKRKIAETSNVITETLPSVETEPVEIEVEFAGSTIELEEGGIEEVVDMGKGVKGSQASSDDSALALLADITSKYQQGEQTLHIVKKDVEVVAQEDAPTPKSLENIEVVEVQISHLNNLFRCDKCDRSFKLFYHLKQHMKVHSDKPFVCPHCNKTYTRESALKQHLNCNHFEAEQSRRQRPQKKMHVCEYCEKQFDHFGHFKEHLRKHTGEKPFECPDCHERFARNSTLKCHMAACQNGVGAKKGRKKLYECQVCSSVFNSWEQFKDHLVIHTGEKPNHCTLCDVWFTQPNDMRVHLRDFHAIHEKVVTEVVIAEPGASEALLEEVQVEPEQMEAGQVVEAVQVETVEGEQGKVEAGVDLRHGEEAEEVTVAPEEPRGSEPDPGQQLQFKVEQISGSAVQHYLEQTVQDTEVPEEQEQVEIHVEETEISEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNC2</accession>
    <name>W5MNC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein naked cuticle homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Cell autonomous antagonist of both the canonical and non-canonical Wnt signaling pathways.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Cell autonomous antagonist of the canonical Wnt signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the NKD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNC2"/>
    <dbReference type="FunCoup" id="W5MNC2">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009881"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009893.1">
        <property type="protein sequence ID" value="ENSLOCP00000009881.1"/>
        <property type="gene ID" value="ENSLOCG00000008140.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688256"/>
    <dbReference type="CTD" id="794221"/>
    <dbReference type="eggNOG" id="ENOG502QWMF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033589"/>
    <dbReference type="HOGENOM" id="CLU_035610_1_0_1"/>
    <dbReference type="InParanoid" id="W5MNC2"/>
    <dbReference type="OMA" id="RQEHHGK"/>
    <dbReference type="OrthoDB" id="5953812at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008140">
        <property type="expression patterns" value="Expressed in embryo and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000579">
        <property type="entry name" value="NKD2, WNT signaling pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040140">
        <property type="entry name" value="Nkd-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611">
        <property type="entry name" value="PROTEIN NAKED CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611:SF1">
        <property type="entry name" value="PROTEIN NAKED CUTICLE HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="162"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="267"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="52297" checksum="8F62021751F0FCDB" modified="2014-04-16" version="1">MGKPQSKHACKQRENPEGHSFVVNARRGLEEGERYGAPEHKIKDGQELQSGELKDGQFFEHHCPLEVVLPPEKAEGCESYTQYIHQEDGEKEITKDLCKGSGRKRINLDELECDVSVEDDNRQEWVFTLYDFDNSGKVTKEDMSSLMHTIYEVVDASVNHSSNSSKTLRVKLTVTPEPSGKKKDASGPGLDREAARYRGEPAHLEDERCGDRRLSTHIRKPNNDQPPHNEGNHYCVDENTERRNHYLDLAGIENYTSRFEAGSPPLQLRQEQHGKSSHSQSRSRSQEAEAHNIQHRRSQVLGDYFPLLGDPLNRGLEGQLRGKGLEKQFLKSPKGTSRGPGVLGGTGGGKSSKCHTYYAPHATSQAGIGGQDVYHLQPQQQPPPSVQLPQQHSHKKLRPKGREVLSPSKGSHCQPAVAEQEPKRDYSAAHHNGGYIMPVVQRHEHHHHHEHHHHHHYHHYHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNN9</accession>
    <name>W5MNN9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNN9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010010.1">
        <property type="protein sequence ID" value="ENSLOCP00000009998.1"/>
        <property type="gene ID" value="ENSLOCG00000008235.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S7N7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234540"/>
    <dbReference type="HOGENOM" id="CLU_066452_2_0_1"/>
    <dbReference type="InParanoid" id="W5MNN9"/>
    <dbReference type="OMA" id="YCWSLIG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008235">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063:SF4">
        <property type="entry name" value="CD48 ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063">
        <property type="entry name" value="LFA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="96"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="18311" checksum="748476CEDE4BAED6" modified="2014-04-16" version="1">IVGESVTFPSVQVSQIIDLKQGDSASLRTVAEVINGTITNTQPDFKDRVTWDNQSGFFQIRDLRTSDSGVYTVQSLGTQKTSTTFNLTVFNRVSRPQVSRVSQVSDSCSLLCSVSNGREVTLSWQREGETLTNTSNPDLNTPLTLPLETEGLSHSYSCVASNPVSTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP02</accession>
    <name>W5MP02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Activator 1 38 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Activator 1 subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Replication factor C 38 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA. Forms an heterotetrameric complex with RFC2, RFC4 and RFC5; this complex has ATPase activity but is not stimulated by PCNA. The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17. Interacts with CNTD1; this interaction facilitates crossover formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP02"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010111"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010123.1">
        <property type="protein sequence ID" value="ENSLOCP00000010111.1"/>
        <property type="gene ID" value="ENSLOCG00000008321.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697996"/>
    <dbReference type="KEGG" id="loc:102697996"/>
    <dbReference type="CTD" id="5983"/>
    <dbReference type="eggNOG" id="KOG2035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075006"/>
    <dbReference type="HOGENOM" id="CLU_042324_5_0_1"/>
    <dbReference type="InParanoid" id="W5MP02"/>
    <dbReference type="OMA" id="LKADIMH"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008321">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000030">
        <property type="entry name" value="replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="34"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="356" mass="40307" checksum="8674DAAF73441392" modified="2014-04-16" version="1">MSLWVDKYRPTSLSKLDFHKDQANMFKNLVQGGDFPHLMVYGPSGAGKKTRIMCLLRELYGAGVEKLRIEHQSITAPSKKKIEINTIASNYHLEVNPSDAGNSDRVVVQELIKTVAQSQQIQTSTQREFKVVLLTEVDRLTKDAQHALRRTMEKYMATCRLILCCNSTSKVIAPIRSRCLAVRVPLPSVEEVCSVLTSVCRKEGLILPGELAKQIAEKSGRNLRKALLMCEACRVQQYPFSVDQEIPETDWEVYLRETANAIVSLQSPQRLFEVRARLYELLTHCIPPEIIIKGLVSELLSNCDGHLKADVAQMAAYYEHRLQLGNKAIYHLEAFVAKFMAIYKKFMEDGLEGMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPB9</accession>
    <name>W5MPB9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has exodeubiquitinase activity and has a preference for long polyubiquitin chains. May play a regulatory role at the level of protein turnover.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MINDY deubiquitinase family. FAM63 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01028173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPB9"/>
    <dbReference type="FunCoup" id="W5MPB9">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010228"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010241.1">
        <property type="protein sequence ID" value="ENSLOCP00000010228.1"/>
        <property type="gene ID" value="ENSLOCG00000008413.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2427">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016607"/>
    <dbReference type="HOGENOM" id="CLU_022566_3_2_1"/>
    <dbReference type="InParanoid" id="W5MPB9"/>
    <dbReference type="OMA" id="TFSKECT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008413">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140934">
        <property type="term" value="F:histone deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF7">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="9">
        <location>
            <begin position="186"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="548" mass="58817" checksum="379A76DE3EC0C519" modified="2014-04-16" version="1">MADHSAETLGHPQLQAASKQDPPETGPGEATKPGPAAEPPFSEWEASRHPGSPSLKAGGEAGRPALGQAGAEEPRGPEAADGARQEGPAARGPDDQPGAEEEGPGGDRSDDNSPSEAESGSLSAPSLGLSEGDGCYLDEPLSSCSAPGPEEASEAGAGVAHPVADASAAGAAAAAVAQEPGAAPAYYFVKWITWKGKKTPIVTQSENGPCPLLAIMNILFLRWKAKLPAQTEVITTEELMAHLGECILSIKPREKTEGLELNFQQNMSDAMAVLPKLSTGLDVNVRFTGVSDFEYTPECIVFDLLDIPLYHGWLVDPQSVEMVQAVGKLSYNQLVEKIIDYKHSSDSSKVSEGLVAEQFLESTATQLSYHGLCELSTTAREGELSVFFRNNHFSTMIKHKGHLYLLVTDQGFLQEEGLVWESLHNVEGDGNFCDSDFLLCHPPPRASPAPPLTAAQAQAQQRQIDQDYLVAMSLQQQSPPPISDLELARQLQQEEYQQQQAQPAQPPPQVLRPRIIVSLFQVRGQASGRQAAERRRPEKKEDSDCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQH7</accession>
    <name>W5MQH7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proprotein convertase subtilisin/kexin type 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="35">Proprotein convertase 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">Subtilisin/kexin-like protease PC9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="42 43">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="43" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="42" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="42" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer. Can self-associate to form dimers and higher multimers which may have increased LDLR degrading activity. The precursor protein but not the mature protein may form multimers. Interacts with APOB, VLDLR, LRP8/APOER2 and BACE1. The full-length immature form (pro-PCSK9) interacts with SCNN1A, SCNN1B and SCNN1G. The pro-PCSK9 form (via C-terminal domain) interacts with LDLR. Interacts (via the C-terminal domain) with ANXA2 (via repeat Annexin 1); the interaction inhibits the degradation of LDLR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 36">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQH7"/>
    <dbReference type="FunCoup" id="W5MQH7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010636"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010651.1">
        <property type="protein sequence ID" value="ENSLOCP00000010636.1"/>
        <property type="gene ID" value="ENSLOCG00000008732.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043472"/>
    <dbReference type="HOGENOM" id="CLU_011263_11_0_1"/>
    <dbReference type="InParanoid" id="W5MQH7"/>
    <dbReference type="OMA" id="GEEMMGC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008732">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043523">
        <property type="term" value="P:regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.690:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000004">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000016">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.690">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041254">
        <property type="entry name" value="PCSK9_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041052">
        <property type="entry name" value="PCSK9_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041051">
        <property type="entry name" value="PCSK9_C3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF60">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18459">
        <property type="entry name" value="PCSK9_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18464">
        <property type="entry name" value="PCSK9_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18463">
        <property type="entry name" value="PCSK9_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="22" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="25" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="29" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="23" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0967">Endosome</keyword>
    <keyword evidence="31" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="26" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="21 36" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="33" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17 36" id="KW-0645">Protease</keyword>
    <keyword evidence="43" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0964">Secreted</keyword>
    <keyword evidence="24 36" id="KW-0720">Serine protease</keyword>
    <keyword evidence="19 37" id="KW-0732">Signal</keyword>
    <keyword evidence="32" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="30" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="16" id="KW-0765">Sulfation</keyword>
    <keyword evidence="28" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="37">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868587" description="Proprotein convertase subtilisin/kexin type 9" evidence="37">
        <location>
            <begin position="24"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="38">
        <location>
            <begin position="168"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Proprotein convertase subtilisin/kexin type 9 C-terminal" evidence="39">
        <location>
            <begin position="440"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Proprotein convertase subtilisin/kexin type 9 C-terminal" evidence="41">
        <location>
            <begin position="525"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Proprotein convertase subtilisin/kexin type 9 C-terminal" evidence="40">
        <location>
            <begin position="583"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="36">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="36">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="36">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00011841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00019781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00032525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00032870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="Pfam" id="PF18459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="Pfam" id="PF18463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="41">
        <source>
            <dbReference type="Pfam" id="PF18464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="43">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="668" mass="72079" checksum="01C470F0E330456E" modified="2014-04-16" version="1">TERLEKMSRLLGIMVVLFSITLGSEIDSEDDEMILSLILQDDTEPEPGAEPLAPFYRCNKDAWRLPGQYLVVMREGTHQPQVERTIRRLGAKAAKRGYLTDTVQTFSGILKGFLVKMSSDVLHLALKLPHVAYIEEDSTIFAQSIPWNLDRIVQTQHAADKYTPPNDGSQVEVYLLDTGVQNSHREIEGRVLVTDFNSVPEEDGIRVHRQASKCDSHGTHMAGVVSGRDSGVARGASVRSVRVMNCQGKGTVSGALAALEYVHASQIAQPYLPLILLLPFVGGFSPTLNGACREMVKSGVMLIAAAGNYRDDACLYSPASEPQVITVGATNYRDQPMSIGNSGTNFGRCVDLFAPGDDIVSASSDCPTCFTSKSGTSQAAAHVAGIAAVILNSTPNITASELLQKLLRYTIKHAIDKNAFPEEHWLATPNMVARLPPASTTGEELLCRSVWSQKSGVSRSATVTARCHPDEELFSCSSFSPSGLRRGEHIEEQEGKKECVAHNAFGGQGVYAVARCCTWERTQCQVRSSEGMGVECLREDHVLTGCSSHSSSGSITDVVRPLHGEKKACSGKEGVVSHASCCHAPRLDCRVKEHTPPGFSEQVLVTCEEGWTLTGCNAVSRGSISHGAYARDNTCVVTSSEGGKGVSAIATCCHNSQSQNNRVMNNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR92</accession>
    <name>W5MR92_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Solute carrier family 7 member 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR92"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010901"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010917.1">
        <property type="protein sequence ID" value="ENSLOCP00000010901.1"/>
        <property type="gene ID" value="ENSLOCG00000008941.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155349"/>
    <dbReference type="HOGENOM" id="CLU_007946_15_7_1"/>
    <dbReference type="InParanoid" id="W5MR92"/>
    <dbReference type="OMA" id="IACQPTE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008941">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903352">
        <property type="term" value="P:L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF28">
        <property type="entry name" value="HIGH AFFINITY CATIONIC AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="599"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="15">
        <location>
            <begin position="572"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="637"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="661"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="674" mass="73413" checksum="087DC44F43E9906E" modified="2014-04-16" version="1">MLNSSARLPTQPHLTAATMACKMLLGFGKQLLRVKVVDCNSEESRLSRCLDTFDLIALGVGSTLGAGVYVLAGAVARDNAGPAIVLSFLIAALASVLAGLCYAEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYVIATSSVARAWSATFDELIGKHIEEFCQAYMSIKAPGVLAEYPDIFAVLIILILTGLLAFGVKESAMVNKVFTCINILVLMFVVVSGLVKGTLKNWRLTQEDMTNLTNSSTYNVSERTASQWIYGDGGFMPFGFTGVLSGAATCFYAFVGFDCIATTGEEVKNPQRAIPIGIVASLLICFVAYFGVSAALTLMMPYYMLDKNSPLPVAFKYVGWEGATYAVAVGSLCALSTSLLGAMFPMPRVIWAMADDGLLFKFLAKISQRTKTPVTATLTSGVIAAIMAFLFDLKDLVDLMSIGTLLAYTLVAACVLVLRYQPEQPSLVYQMASTLDESEMTESVSNSESQAGILPDGMEEFSMKRMLFPENSDPSSMSGLIVNICASMLGLLVFIFCIIAVQGGTKSWSVALLTIFLIMSVVITIIIWRQPESKTKLSFKVPLLPFLPVLSMFVNVYLMMQLDRGTWIRFLVWMVLGFIIYFGYGMRHSTEAALAQSATENGYKQARMSNGEPTVPEKEAFLNDPDELDARNHWQEEEGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRG5</accession>
    <name>W5MRG5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium channel, subfamily K, member 13a</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632196.1">
        <property type="nucleotide sequence ID" value="XM_006632133.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRG5"/>
    <dbReference type="FunCoup" id="W5MRG5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010974"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010990.1">
        <property type="protein sequence ID" value="ENSLOCP00000010974.1"/>
        <property type="gene ID" value="ENSLOCG00000009008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695729"/>
    <dbReference type="KEGG" id="loc:102695729"/>
    <dbReference type="CTD" id="571467"/>
    <dbReference type="eggNOG" id="KOG4404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157960"/>
    <dbReference type="HOGENOM" id="CLU_022504_3_1_1"/>
    <dbReference type="InParanoid" id="W5MRG5"/>
    <dbReference type="OMA" id="NFTRRHN"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009008">
        <property type="expression patterns" value="Expressed in bone element and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000070">
        <property type="entry name" value="Potassium channel, subfamily K, member 12 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005410">
        <property type="entry name" value="2pore_dom_K_chnl_THIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF57">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01588">
        <property type="entry name" value="THIKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="93"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="200"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="45996" checksum="E4159E32CE6E9A19" modified="2014-04-16" version="1">MANRGCCCCSVGPVPEDTARFLLLALLIVLYLLCGAAVFSALEHPKEKQAKERWAQRLDNFTQRYNLSREDLERFLRHYEEANVAGIRVDSVRPRWDFTGAFYFVGTVVSTIGFGMTTPATVGGKIFLIFYGLFGCAATILFFNLFLERLITVLAFVLKSCYERQRRRQGVAPHEHRRDSSHSEGADSLAGWKPSVYYVMLILCVAAVIVSCCASAMYSAIEGWSFFDSLYFCFVAFSTIGFGDLVSSQNMKYEDQGLYRFGNFVFILMGVCCIYSLFNVISIVIKQILNWILRKLEVRCCPRCPARRLRARRNVVVPGRPRSRRNVSIETDAINESETDGRRMSGEMISMKDFLAANKVSLAIMQKQLSETANGHPRPPGCGSRHNGFSGGVGALAIMNNRLAETSVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSJ9</accession>
    <name>W5MSJ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dishevelled-binding antagonist of beta-catenin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dapper family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSJ9"/>
    <dbReference type="FunCoup" id="W5MSJ9">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011358"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011374.1">
        <property type="protein sequence ID" value="ENSLOCP00000011358.1"/>
        <property type="gene ID" value="ENSLOCG00000009312.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVXB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183181"/>
    <dbReference type="HOGENOM" id="CLU_021211_1_0_1"/>
    <dbReference type="InParanoid" id="W5MSJ9"/>
    <dbReference type="OMA" id="HKRGDYR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009312">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046329">
        <property type="term" value="P:negative regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000095">
        <property type="term" value="P:regulation of Wnt signaling pathway, planar cell polarity pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024843">
        <property type="entry name" value="Dapper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919:SF12">
        <property type="entry name" value="DAPPER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919">
        <property type="entry name" value="DAPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15268">
        <property type="entry name" value="Dapper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="466"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="596"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="603"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="821" mass="89950" checksum="D93E050576D8A516" modified="2014-04-16" version="1">TAPVAAMSVSKECLLARDRLDSETRCRERVDGELDRWRTRERLEATLAGLGELEYLRQRQELLVRSVLARQERAAFAGEATASGAAEDAFLNSEEKLLEENILLLRKQLNCLRRRDAGLISQLQELDRQISDLRLDSEKVSIDHLETDSRPSSGFYELSDGTSGSLSNSSNSVFSECLSSCRSSSCFCNPLDTSLGAADGQPRPADELVGCADCEGRCEDQSSVLVRRSFSAPYSPSVDGTPEGQSKYQCDLVARNGSDVYRYPSPLHAVAVQSPMFFQSLVGHFKEDGIQFKASEPSNEFLKPEQAPEPHNVSWPASYQPSNKRLDHYILGLLQRRAQPVRTNKPRTSINTDPAKSILRQGSICVRQTVGAAQGKTPDLKASRQACLHSSATGSADVSAASPMKQWPAEATIDQLENGKSLVPSAYHQHSEHLSNTIEIHTNSLSKKNTSVANKGLPLSTVTKDCQEMGAPHSTSSPKDSGNRYLPMPDDRKGSQSPRQSLKSPPAVPPPLEKRPPLELRSLGSSSQSQDEGGRLVSAQYIPAQRQAGKLRKGGRNVKIVKVKGAALKTRANIEPIPEVSQEKPRVVAKKCRFPDDIPNLHKASKKSSSKTKRVGSSHLDSSLAGRYPVSTGKSAAPRHHGHGREVILTKPKYKRADYRRYKTIAEVPYEEAMRKARRRQKREVLGQVSAVYLPPTVQYASPYSYVGSDSEYSAECASLFHSTVLDTSEDEQSNYTTNCFGDSESSLSEVDFVGESTTSSDSDESGGLVWPQFGQSIGPVVPSEVTSAQAKAFVKIKASHNLKKKILRFRSGSLKLMTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTE4</accession>
    <name>W5MTE4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">A-kinase anchoring protein 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011653"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011670.1">
        <property type="protein sequence ID" value="ENSLOCP00000011653.1"/>
        <property type="gene ID" value="ENSLOCG00000009529.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSMH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00810000125473"/>
    <dbReference type="HOGENOM" id="CLU_231193_0_0_1"/>
    <dbReference type="InParanoid" id="W5MTE4"/>
    <dbReference type="OMA" id="INCPPIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009529">
        <property type="expression patterns" value="Expressed in muscle tissue and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000113">
        <property type="entry name" value="A-kinase anchor protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF2">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="476"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="522"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1204"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1269"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1318"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1887"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1229"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1276"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1293"/>
            <end position="1302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2061" mass="227911" checksum="F5A341DC9909B2B4" modified="2014-04-16" version="1">VFAMSIAVSPMATEAVSPMITTVTPTVEPRKAAEEEEEEGRGGELAKEAGPAQRYDKPPPLHTGADWKVVLHLPEIETWLRCTADRVRDLTHSVHQDAHNKHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSVNIIVDIRTVQLLWHQLRVSVLVLKERVLQGLQDSNGNYTRQTDILQAFSQDQTEARLDALTEVDDSGQLTIKCSQNYLSLDCGITACELSDYSPSEDLPIELEAMTSSRTKAKTHETCFYPDLEKDFPELIQSVGLLAVAAERLSSKQDESPVSGQDTPLSSTPQTREESKATSGKPPLPQGNTEPPGEDLALADNVVMSPIKHQGVPRSRHASHCENPLSKRPIRDCFNYNEVSPTQPSMPKKAMYLEGMSSDDTESIIKRAPPSSLQFKGDMSRSTPSLLDPPDRSKFWLELTSVYPRSAPSAVSQSYDCLNTMSDKIHKGLANGTLYKRVQGQAASNPSLQGSTSEAEQPSSLADHPSPPEQLGCAHVVGFQESCNEMKSSPLNVSSPEGSDLTPPDHRKGLSATIPETSAHSPDSKSLPVATKEHLPSTCNGTETKKEPWYGSDEYLALPSQLKKTEMLAMKLENLAKVLPQKPMEETFQDVDDWELSEVNSDCDGYLPLRLTRVSEKPFPGGRLSPTSSSDIAPSLDESIESGPLSDLLSEDEVNAADLSVKQKARDLWLVKDPSQAEETSLGRTALIQQLLDDIQHQENYEDIWEKIEGFVGKLDEFIQWLHEALETTENWTPPKAETTCLKLYLETHLSFKLNVDSHCALKDTVVEEGRQLLEFIVSHKSGLKDMLQMIVNQWQELQRQIRRQHSWILRALEIIKAEILASDVSHEAESGAGSPEGEAQRWHHETQQDVLEQLSLRVHSERFSSRTERKREFAEMSKVNRIGSNSLLDFESEYQELWDWLMDMESIVMDSHDLMMSEEQQQHLYKGNSVEMAMWFPRKTQLLSRVESLRRSATSLPADFEDRVSALTQKWDLLEKTLEERTGGQTGAQAGLGPCSLLSPETNGLVRQLESRIKELRGWLRDTELLIFNSCLRQEKAEAEAPHAPKQLQYFKSLCGEIRQRRRGVSSVLRLCQRLLEEQDSSTPDTERQALQLVTVNLERRWEAIVMQALQWQTRLQKRLGTEQVPGNVIEPAFMDLNTSAEDSWEWDDTDISNDLISVNMEPHDLEQVESHTSKLTVKSPGSRAGDPQFDQYVSQHDSPSCGSGFTPPSSSPRVYQVYSLHNVELYERPHLPFLGKSPQDSSQKQPNTLMKSLSKDSSFSSTESLPDLLGGVGSVNQGGLACSGDSARRSESESGIVSEGDTETTANSEVCPPNRVDEPRGELSSEPPVGVDEPESSLTSNEVKMQSALNRSGSGGIVEHVDVSVSCTSRGILQANYQKTPAEKKKRASIDQPSEGHPVAIILNGQEPLAEGDSEGFKQPLFTRQDDERGCADLKSGKQSAFLSHGSSLDSLSVAGDLFLSGKDTLHRSTSLESWLAPCKSTEEISSQHSLGDLGFGGDSTGELSKRTLELLKRLENIPSPFNHKIKRSISDITLQSSSLKMPASTQLPLEVASSVNEDSAASLTELSSSEDLSLCSEDIAVQKNRIVDSNASFRKHVNHVVPDEADVSISMIVNVSCTSACTDDDEDDSDLLSSSTLTLTEEELGIKDDDDSSIASDEEYIESSFALGLDYMKNELQNWIKPRISQSKEKNELGDELQCGALSRENITSSFTANERRFLNRSALKLLEANANGKSDSLRPKASDISKVSQEKSRSYMNQFVDDMENGNVENSQLKAKDADDELLREEESLITKSGESLKDCYASDLGTGSSVGVVTGAKSFQCDLLSTQPKESSLECQLSGEIPCCSSDREPSSVLPMSNRQGSHKGTVELTHNDGISDSFAIFRKDAEEISDSIAEVPSCCSHHSPVEEGKNEDDGSVHNFVMEIIDMASVALKNKTLSDPQAPSPTPVAQIRDKVLEHSHRPIQLRKGDFYSYLSLSSHDSDCGEVSTCVEDKSSTPLPPDLGDTLNMRDEELLFKPCTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTW8</accession>
    <name>W5MTW8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sec1 family domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Syntaxin-binding protein 1-like 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">Golgi stack membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011827"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011847.1">
        <property type="protein sequence ID" value="ENSLOCP00000011827.1"/>
        <property type="gene ID" value="ENSLOCG00000009663.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074845"/>
    <dbReference type="HOGENOM" id="CLU_016216_3_1_1"/>
    <dbReference type="InParanoid" id="W5MTW8"/>
    <dbReference type="OMA" id="VNDLRAW"/>
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        <property type="component" value="Linkage group LG7"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
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    </dbReference>
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        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Sec1 family domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="sec1 family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="698" mass="78540" checksum="116B611AE3505059" modified="2014-04-16" version="1">MYQCVFGLWQKTYTNGAKTYKLHAGSTPGTEPRAASYKMQTLPAGRSSASCHVTRCSSLSDSETAKMAASIREKQTAALRRMLNFNAPPLKNSAAEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAIYFVMPTEENVDRICQDLRNQLYESYYLNFISAISRSKLEDIAGAALGANAVSQVTKVFDQYLNFITLEDDMFVLCNQNKELISYHAINKPDIMDTEMDAIMDTIVDSLFCFFVTLGAVPIIRCPRGNAAEMVAVKLDKKLRENLRDARNSLFTGDTMGAGQFSFQRPLFVLADRNLDLATPLHHTWTYQALIHDVLDFHLNRVNLEESSGPEASPSGARPKKKNKKSYDLTAADRFWQKHKGSPFPEVAESVQQELDTYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLDNIKTRKLDVYFEYEEKLMSKSTLDKSLLDMISDPDAGTPEDKMRLFLIYYITSQQPPTETDLEQYKRALTDAGCNLAPLNYIKQWKAFTKMAATPASYGNSGTKPMGLISRVMNTGSQFVMEGVKNLVLKQHNLPVARILDNLMDMKCNPETDDYRYFDPKMLRGSESSIPRNKNPFQEAIVFVVGGGNYIEYQNLVDYAKTKPGKRVMYGCSELFNASQFLKQLSQLGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVY7</accession>
    <name>W5MVY7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVY7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012546"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012569.1">
        <property type="protein sequence ID" value="ENSLOCP00000012546.1"/>
        <property type="gene ID" value="ENSLOCG00000010254.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107078822"/>
    <dbReference type="eggNOG" id="KOG0181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00860000133854"/>
    <dbReference type="HOGENOM" id="CLU_348129_0_0_1"/>
    <dbReference type="InParanoid" id="W5MVY7"/>
    <dbReference type="OrthoDB" id="6162046at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010254">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031440">
        <property type="entry name" value="DUF4670"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21937">
        <property type="entry name" value="CCDC66 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21937:SF5">
        <property type="entry name" value="GENE 973-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="810" mass="90361" checksum="702116AE4A76B165" modified="2014-04-16" version="1">MKDSALSLLSRGCGHLVKKEDHSTDGKLEVYFEPKDYFNWKSQQAGFDLSKTEKGHEAPYKAEPAPPKTYSTRKGPLILYSEDMALSSWDTGRPGRKRKGNHRYRQEVELQLRTLRDLTGAILAYGNKQEGPGDAQPLAHFLGNPQEEQMDGQIRPGYCAKRCLAHLSHTWHPNANFSSAGQLPGGTLDPSGVPDNPPSSPSLYRNECDLTNEPRLSNVFQDSLPHVPEVGECRIDMPDKETNGPFRDKQNVDTKQLEDQKAGRKRRKRCSIMPEAPRAGLSPVPEMELPFLEENTGDTGGLKETLVCGAPQPWDGVFEERQNEAPKKEVTVTCSMKTTSIDSSQLHSQRSYITYYGGTLAGGKKSSVSKSSRNDQRDRQDKTLLDPSSLDLPFPPIPTGMVSMLHPMNETAETESDRLVEEGAELLTLPALFVPESTRKSNRKKCPEKDFPKEMLILPMLFPGDAAKGYGQKSEDVSHHAEPSQMKVDLHGSPEQEVEKRFEGASAEEMPRALPVLRPREVELEWSGQRHTADRTELKEDNQDDCFTAGVLPPIIGKKGPGRQSSMAYFRDPSKDQKDSQTGIIRGSIPEVLRESYRGSSVGCLIMAPDGEIMRLSLWGPITENGEDLLFDDITQNEALQILSSDVIVEQPWKIFLQPGTEMPTVGLDTDSDFLTRQSQDMSTYLNKSDKIEAGENGHEGHKKSGERSNPDTSDRVDRVLIKDKESNRNFRKNKLVNHEWANQHLQKPGKMKPHEYWDSKGNNEASQKTAGKILPTEGIASTTQTRRVGDPLQKAKKHPDDATCCKCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWR5</accession>
    <name>W5MWR5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01001298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWR5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012824"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012850.1">
        <property type="protein sequence ID" value="ENSLOCP00000012824.1"/>
        <property type="gene ID" value="ENSLOCG00000010446.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160151"/>
    <dbReference type="InParanoid" id="W5MWR5"/>
    <dbReference type="OMA" id="PFRPDPN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010446">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08750">
        <property type="entry name" value="RGS_GRK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001100">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF14">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="60"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="187"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="450"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="576" mass="67020" checksum="0F68C8E36CBC3338" modified="2014-04-16" version="1">SYIILYMIQPQTQCLHHRGGGGKRNGRSKKWKEMLKLPHISLCEELRRTIERDYSSLCEKQPIGRLLFRQFCDTRPELKRCIEFLDAVVGYEVTPDEKRRDCGLNILDTYFNNGSAAHLPEIPQEVVRDCREKLDQSPCKELFKECTRIVHEYLSGAPFSQFQDSMYFSRFVQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIFEMIQGQSPFRRRKERVKREEVDRRVREDPEEYSEKFSEEAKDICRALLAKDPKERLGCQGGGGAEVKQHRIFKNINFKRLEANMLDPPFIPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVYEADGTLCPDLDVNRPNPPPKRGFFYRLFSREVCFKSGYSDDEEEPYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWY8</accession>
    <name>W5MWY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zgc:194242</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015197628.1">
        <property type="nucleotide sequence ID" value="XM_015342142.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWY8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012924.1">
        <property type="protein sequence ID" value="ENSLOCP00000012897.1"/>
        <property type="gene ID" value="ENSLOCG00000010529.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688230"/>
    <dbReference type="KEGG" id="loc:102688230"/>
    <dbReference type="eggNOG" id="KOG1269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010372"/>
    <dbReference type="HOGENOM" id="CLU_081534_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWY8"/>
    <dbReference type="OMA" id="QTHYFWP"/>
    <dbReference type="OrthoDB" id="10250730at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010529">
        <property type="expression patterns" value="Expressed in larva and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000817">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="57"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="24444" checksum="ABCCD29A2186DCA6" modified="2014-04-16" version="1">MSELIYGILSKKLNRHLGRPAKSLTGWLLTKFFIWHNRVLEENAVKLVEIQPDDMVLELGFGLGLGLQAAAPLLTGPRGKLFGVDYSEYMFAKASERMRAEIESGKVTLFNGNIMAMPLPDSSVDKVYHCNCYYYWPDLKGMTSEIHRVMKPGGLMVTTLILSSITKAASRRILLNDNWKPEVYMEALRATGFSDVRMEDKQDKNIAFQAIFATASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYG9</accession>
    <name>W5MYG9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Paraoxonase</fullName>
            <ecNumber evidence="13">3.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a phenyl acetate + H2O = a phenol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140310"/>
            <dbReference type="EC" id="3.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10 13">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the paraoxonase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.2" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01004630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYG9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013457.1">
        <property type="protein sequence ID" value="ENSLOCP00000013428.1"/>
        <property type="gene ID" value="ENSLOCG00000010932.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695084"/>
    <dbReference type="KEGG" id="loc:102695084"/>
    <dbReference type="GeneTree" id="ENSGT00390000008932"/>
    <dbReference type="HOGENOM" id="CLU_049839_0_1_1"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010932">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000023">
        <property type="entry name" value="Serum paraoxonase/arylesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
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        <property type="entry name" value="Arylesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01731">
        <property type="entry name" value="Arylesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01785">
        <property type="entry name" value="PARAOXONASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 12 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="12">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="In form B" evidence="11">
        <location>
            <begin position="43"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008595"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="355" mass="39697" checksum="CFCE50FD7CF09E63" modified="2014-04-16" version="1">MGKILIVTAIAGVLLAFLGERYMTFRTKLMASRDIISNHLPNCVYIKGLEYGSEDLTILPGGLALISTGLKYPNLRRLSDDPGKIYALNLEEGNLKPVELKISRGFDLDSFFPHGISTFIDEKDQTVYLFVVNHPDFKSTVEIFRFVEEDSSLTHLKTIEHELLHSVNDIVAVGPESFYATNDHYFTNALFSVEYMIGLALCNVVYYSPEEVREAAAGFYSANGINISPDKKHLYVGDVFDHVVHVLEIQENKTLVPLKKVDVGTLPDNIEVDSVTGDLWLGCHPNAWRLLRYDPENPPGSEVIQIQNIHSEKPTVTKVYVDDGHVIEGSSVATPYKGKLFIGTMYQKAIICDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYL6</accession>
    <name>W5MYL6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clusterin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYL6"/>
    <dbReference type="FunCoup" id="W5MYL6">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013475"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013504.1">
        <property type="protein sequence ID" value="ENSLOCP00000013475.1"/>
        <property type="gene ID" value="ENSLOCG00000010985.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ44">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063668"/>
    <dbReference type="HOGENOM" id="CLU_042162_0_0_1"/>
    <dbReference type="InParanoid" id="W5MYL6"/>
    <dbReference type="OMA" id="YLSEDCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010985">
        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF2">
        <property type="entry name" value="CLUSTERIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868502" description="Clusterin" evidence="9">
        <location>
            <begin position="24"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="10">
        <location>
            <begin position="29"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="11">
        <location>
            <begin position="252"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="470" mass="53530" checksum="295A5F1AF6390E41" modified="2014-04-16" version="1">RSLKMRWLLVLVMCSTLLGVLQSIPRTNQEGLSKDTLKKLSKDGQKYVDEEMKKALFGVKQMKDIMEMNEEKHEHLMSSLRHSSEKKKGAVQLAKEVEQQLGVAEQHCQASLRSAWEECRPCLEQTCTSFYTSRCRRGFSTFTAKVEEFFSKMSSQFSSHDNQDLLFKQSPETFDTEVAQIENSFHQLLSEVNSLYEKSVMLVTKMQKQFDQALQAAFTSDLRSKELVEKPLYPSQQTPDSGFFEGLGLEDVLESFFDFGRNVFEEFSSVITQAFGDLQESVTEETNREKEGELSLQVLPSQSRQLCRDLHRQSSECWQFQSRCQSCQKASQGTECPSVTELHSELNEISYLVNVSSQQYEEVLQILQHHTEDTVGWLTNMAAYFGWVAELANSTVEPESVFTITTVVPHPQDGESNFDSDTTVEVNILNSPTFTLSVPGDLEIQDPDFVKYIAQEALGMYKQLVRQDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N013</accession>
    <name>W5N013_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="42">Triacylglycerol lipase</fullName>
            <ecNumber evidence="42">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="42">Pancreatic lipase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="45 46">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="46" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="45" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="45" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:39956"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1,2,3-tributanoylglycerol + H2O = dibutanoylglycerol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:35020"/>
            <dbReference type="ChEBI" id="CHEBI:76478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:76978"/>
            <dbReference type="ChEBI" id="CHEBI:88066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:47865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2,3-tripropanoylglycerol + H2O = dipropanoylglycerol + propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:88153"/>
            <dbReference type="ChEBI" id="CHEBI:88155"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:48025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1,2-didecanoylglycerol + H2O = decanoylglycerol + decanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48596"/>
            <dbReference type="ChEBI" id="CHEBI:11152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:90605"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2-didodecanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = dodecanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48516"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90337"/>
            <dbReference type="ChEBI" id="CHEBI:90359"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:48517"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1,2-didodecanoyl-3-beta-D-galactosyl-sn-glycerol + H2O = dodecanoyl-3-beta-D-galactosyl-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48540"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90340"/>
            <dbReference type="ChEBI" id="CHEBI:90515"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:48541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2-dioctanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = octanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:90457"/>
            <dbReference type="ChEBI" id="CHEBI:90768"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:48693"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-dioctanoyl-3-O-beta-D-galactosyl-sn-glycerol + H2O = octanoyl-3-(beta-D-galactosyl)-sn-glycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:90453"/>
            <dbReference type="ChEBI" id="CHEBI:90769"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:48697"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-beta-D-galactosyl-2,3-didodecanoyl-sn-glycerol + H2O = 1-beta-D-galactosyl-dodecanoyl-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90342"/>
            <dbReference type="ChEBI" id="CHEBI:90514"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:48537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 2 H2O = 3-beta-D-galactosyl-sn-glycerol + 2 a fatty acid + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13189"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15754"/>
            <dbReference type="ChEBI" id="CHEBI:17615"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.26"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13190"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = acyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28396"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90310"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:48373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a monoacyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:84465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:44665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>di-(9Z)-octadecenoylglycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>long chain 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = long chain acyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90463"/>
            <dbReference type="ChEBI" id="CHEBI:90774"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:48709"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>long chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol + H2O = long chain acyl-3-O-beta-D-galactosyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90477"/>
            <dbReference type="ChEBI" id="CHEBI:90770"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:48701"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Glycolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 42">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Zymogen granule membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 41">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="42"/>
    <dbReference type="EMBL" id="AHAT01017451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N013"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013972"/>
    <dbReference type="ESTHER" id="lepoc-w5n013">
        <property type="family name" value="Pancreatic_lipase"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000014001.1">
        <property type="protein sequence ID" value="ENSLOCP00000013972.1"/>
        <property type="gene ID" value="ENSLOCG00000011362.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUK7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155139"/>
    <dbReference type="HOGENOM" id="CLU_027171_0_2_1"/>
    <dbReference type="InParanoid" id="W5N013"/>
    <dbReference type="OMA" id="EPWVQGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011362">
        <property type="expression patterns" value="Expressed in intestine and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042589">
        <property type="term" value="C:zymogen granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047714">
        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000033">
        <property type="entry name" value="Pancreatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000003">
        <property type="entry name" value="Triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002331">
        <property type="entry name" value="Lipase_panc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF165">
        <property type="entry name" value="PANCREATIC LIPASE-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00823">
        <property type="entry name" value="PANCLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 39" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13 40 42" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 42" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 42" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 39" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="46" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 42" id="KW-0964">Secreted</keyword>
    <keyword evidence="43" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="43">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012475045" description="Triacylglycerol lipase" evidence="43">
        <location>
            <begin position="16"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="44">
        <location>
            <begin position="369"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="38">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="23"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="257"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="330"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="465"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="RuleBase" id="RU362046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="SMART" id="SM00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="481" mass="53028" checksum="FC888BD4802E4EC1" modified="2014-04-16" version="1">MIVRWTILAIYIATATDLGAEVCYDRLGCFGNDKPWAGTVERDISQLPWSPERINTRFLLFTRQNSNSFQDITAINPSTISASNFRSNRKTRFIIHGFIDKGEEGWVTDMCLEMLQVEDVNCICVDWKGGSRCPYTQAANNIRVVGAEVAYFGEVLQTQINYHPANIHIIGHSLGAHAAGEAGKRKPGISRITGLDPAEPYFQNTPIEVRLDPSDAAFVDVIHTDAAPMVPNLGFGMIQAVGHVDFYPNGGEEMPGCNKNAVSQILDFGFGLVSQIVDIDGIWEGTRNFVACNHLRAYKYYTDSIKQRDGFIGYPCSGYGQFQEGNCILCPSGGCPVLGHYADSYNPPVGAINQMLYLNTGDAAPYARWRYKVSVKLSGSRAVSGFINIALYTNEQYTRWLQIFRGKLEPAGTYTAGIDVEVNLGDLTNVKFLWDSSLFNPLQPTLGAETVTAQRGKDGKVFRFCGAGTVREEVLQTLTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0J0</accession>
    <name>W5N0J0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pterin-4-alpha-carbinolamine dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dimerization cofactor of hepatocyte nuclear factor 1-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phenylalanine hydroxylase-stimulating protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Pterin carbinolamine dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. Also acts as a coactivator for HNF1B-dependent transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01017443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203007.1">
        <property type="nucleotide sequence ID" value="XM_015347521.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0J0"/>
    <dbReference type="FunCoup" id="W5N0J0">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014149"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014178.1">
        <property type="protein sequence ID" value="ENSLOCP00000014149.1"/>
        <property type="gene ID" value="ENSLOCG00000011517.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697805"/>
    <dbReference type="KEGG" id="loc:102697805"/>
    <dbReference type="CTD" id="5092"/>
    <dbReference type="eggNOG" id="KOG4073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007221"/>
    <dbReference type="HOGENOM" id="CLU_081974_3_2_1"/>
    <dbReference type="InParanoid" id="W5N0J0"/>
    <dbReference type="OMA" id="WAEKWNH"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011517">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00914">
        <property type="entry name" value="PCD_DCoH_subfamily_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.20:FF:000001">
        <property type="entry name" value="Pterin-4-alpha-carbinolamine dehydratase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002018">
        <property type="entry name" value="PRK00823.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002020">
        <property type="entry name" value="PRK00823.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF13">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="102" mass="11726" checksum="5D0E04E24E6104CF" modified="2014-04-16" version="1">MAGKAQRLTQEEREQVLPHLRSAQWIEVVGRDAIYKEFLFKDFNQAFGFMSRVALQAEKMDHHPEWFNVYNKVQVTLSTHECGGISQRDVTLASFIDQAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0W2</accession>
    <name>W5N0W2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Catenin alpha 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vinculin/alpha-catenin family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014271.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="353" mass="39035" checksum="2C1A2DF3CBAF8A2E" modified="2014-04-16" version="1">MTLLQNLQLKIDPKDLQIRTITVERLLEPLIIQVTTLVNCPKNPSNKKKGCSKRAKVLLKSVEEATLNLIDKGEMIAKETVVLKDELTAAVKEVQRESHSLTWTAKEFTDDPCCLSKREAVVQAARTLLAAVTRLLILADIIDVIYLLEHLSTVQKTFEILRTSSNKIDLKKAYQQFGKELNVLDNLAHKRQQDLKSPDQRDEMASARGLLKQNSSLLYSTSSACLEHPDVASLKASKDSICNEIQDAFTAISNAAQGIGQAKVQPMSTSGTLGGALDELESVIVIDPLTVNEDNIRPALEKRLEAIISGAALLADSSCTRDIHRERIITECNVVRQALQELLSEYIGNKSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1A2</accession>
    <name>W5N1A2_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Ganglioside GM2 activator-like</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG6"/>
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        <property type="term" value="F:lipid transporter activity"/>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="23"/>
            <end position="194"/>
        </location>
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    <feature type="domain" description="MD-2-related lipid-recognition" evidence="3">
        <location>
            <begin position="40"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="194" mass="21491" checksum="ED0021462D00234A" modified="2014-04-16" version="1">MTSLRFTAVLSIFCCFLAFGNTAEVGPRMLPIDKSNSSNWAHCGPKNDLVIKNLNFPNTIYVPGSVTVSLLGELKVPIEAPLRIEVQVKKRAVWFWMKVPCLFKYGSCTYRNICEKLNKVFPGNSCPSRIVSYGGSCRCPIKPGVLNVPPTTFHIPKVRLPSFLAGGTYKVQIKAISGKKEIVCFQTTVSMKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1R4</accession>
    <name>W5N1R4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Armadillo repeat containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1R4"/>
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        <property type="interactions" value="590"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014573"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014602.1">
        <property type="protein sequence ID" value="ENSLOCP00000014573.1"/>
        <property type="gene ID" value="ENSLOCG00000011846.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157476"/>
    <dbReference type="HOGENOM" id="CLU_018048_0_0_1"/>
    <dbReference type="InParanoid" id="W5N1R4"/>
    <dbReference type="OMA" id="LYPMQSR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011846">
        <property type="expression patterns" value="Expressed in mesonephros and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052441">
        <property type="entry name" value="Armadillo-Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46618:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="5">
        <location>
            <begin position="291"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="440"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="EDR1/CTR1/ARMC3-like peptidase-like" evidence="4">
        <location>
            <begin position="720"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="654"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="679"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="872" mass="95226" checksum="82779ECA047B955C" modified="2014-04-16" version="1">MGKKVKKEEEPPAKDVFDPLPIESKSAATVVLMLSSPEEEVLAKACEAIHRYAEKGDENKGTLVGLGAVEPLARLISHEDKVVRRNAIMALGVMAAHSDVKRHLKKLDIIPSVIGRLLPEEDVVIHEFASLCLAYLSVDYTSKVQIFDHDGLDPLIHLLSSPDPDVKKNAVECIYNLVQDFPSRSAVRELNGIPPLLELLKSEFPIIQQLTLTTLALITNDGENRVALRENQGLERLLEFLGAKEFSDLHVEALQVISNCLEDKEAMHLIQETGGLDKLLQFAETATLPEVQMNATKAISRAAQDSENRKILHEHDVEKTLVSLLAVDHDGVRIAACLAIAVMCDLLASKDSFRNHEGVRPVVQLLSSENGEVKEAAALALSKLTQSNQLNAYAVFEAEGEEPLVQQLSDARDGAVAYAAAVLTNMAAQETLRPGILAHGVTPALAEALQSGNSLVLGNAALAVAALACDSEGRAELRNAGGLVPLVKLLNSNNTEVRCNACWAALVCANDEPTAVELCKLGALEILQDINLSSNRRNKFSEAALLKLLDSNLSIKYSLTGYLSPTDLITDGFYDPGQVKPGHRILTLEDLSKQEVNQHRPVILVDGKPHEQSPKAITDHKKFHDGSWVSNNSSLNVTPTVCKTYSSYQRVSTESAPIDEKQQDGLGATRSPSVLSKSSTKEKTKGKNKGKKDDEKQKDEDEIKSPQETITENKPGIPSSDSAFHALVSEAAKAVLPLHDTREQVIGLAKLVADAMGGDVERDKLHEFSWELHLSELKYELKSNIVPIGKIKKGIFYHRALLFKALADRIGINCSLVRGDYNRAWNEVAIVEGSPKVPGPRLQPKTYIVDLIHRPGRLMKNSSPEAIQYQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N200</accession>
    <name>W5N200_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Complexin 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N200"/>
    <dbReference type="FunCoup" id="W5N200">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014659"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014688.1">
        <property type="protein sequence ID" value="ENSLOCP00000014659.1"/>
        <property type="gene ID" value="ENSLOCG00000011926.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RY7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182938"/>
    <dbReference type="HOGENOM" id="CLU_141096_0_0_1"/>
    <dbReference type="InParanoid" id="W5N200"/>
    <dbReference type="OMA" id="SLMGQFQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011926">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031630">
        <property type="term" value="P:regulation of synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22809">
        <property type="entry name" value="Complexin_NTD_CPLX_III_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF7">
        <property type="entry name" value="COMPLEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="180" mass="20469" checksum="346DAD156D2C6885" modified="2014-04-16" version="1">ASKTKRNRVALIKKLDATRTMSFLLKNMVSSQVKNLGLGGGEEKKEESGAPADPAAAAGMTREEYEEYQRQVLEEKMERDAEFAHKKAERATLRVHLREKYRLPKSEQDESMIQIAGDDVDVPEELRKMVDEDAVEEEEKDSILGQMQNLQNMDMDQIKEKAQATFTEMKVAAEQKCSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2B9</accession>
    <name>W5N2B9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Corin, serine peptidase</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2B9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014778"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014807.1">
        <property type="protein sequence ID" value="ENSLOCP00000014778.1"/>
        <property type="gene ID" value="ENSLOCG00000012013.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157103"/>
    <dbReference type="HOGENOM" id="CLU_010853_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2B9"/>
    <dbReference type="OMA" id="TCLMPDQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012013">
        <property type="expression patterns" value="Expressed in heart and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07888">
        <property type="entry name" value="CRD_corin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000015">
        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000083">
        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000024">
        <property type="entry name" value="Low-density lipoprotein RecePtor related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000056">
        <property type="entry name" value="Terribly reduced optic lobes, isoform AM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041763">
        <property type="entry name" value="Corin_CRD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF11">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE-CONVERTING ENZYME ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
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        <property type="entry name" value="LDLRA_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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    <feature type="chain" id="PRO_5004868680" evidence="17">
        <location>
            <begin position="21"/>
            <end position="917"/>
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            <begin position="13"/>
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        </location>
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        <location>
            <begin position="333"/>
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    </feature>
    <feature type="domain" description="Peptidase S1" evidence="19">
        <location>
            <begin position="683"/>
            <end position="910"/>
        </location>
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        <location>
            <begin position="18"/>
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            <begin position="26"/>
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            <dbReference type="ARBA" id="ARBA00004613"/>
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    <sequence length="917" mass="102627" checksum="3D1C1E21D65C4C6B" modified="2014-04-16" version="1">TFCILPGLFFFLILSGPCIDINVSQCHILPYNQTSLSSRSSIVRSVEVEMFLKFFSSLNRLSCYRHIMLFGCSLALPECAGEGDERRLIFPCMSFCEAAREGCEPVLQTFNASWPDFLRCSQFKNSTSMDDSAPICYSPKQTKGKCPAVCGGQDNFLCATGICVPRKLLCNGYNDCDDWSDEANCNCSKDQFQCGTGRCLSHSLVCDGYDDCGDLSDEQNCDCDPNKEHRCGDGRCITADWVCDGDHDCADKSDEVNCSCKSQGLLECRNGQCIPSAFRCDGDIDCKDGSDEENCTLKQNQNACEPGQPGCISTSCLDGCHGNTVCDSRSNSTNCNRCEPITLELCMNLPYNVTSFPNFLGHETQKETSISWESSLFPALVQTNCYKYLMFFACTILVPKCDPETNQKVPPCRSLCKNSKERCESVLGIVGLQWPEDTDCSQFPEEGQENATCLMPDQDVEGTECSPSHFKCNSGRCVLASKRCDGHTDCDDDSDEENCGCKERGLWECPSNKACIRHTMICDGFPDCADLADERNCSFCVDNELPCNNHKCVHRTLWCDGKKDCTDSSDEWNCVSLSKTPSSLLTVHKTASEYHVCADNWHHKLSELACKQMGLGEPAEVEMVPDHHHQLGRRKWLHVHADWKHRNGSSLQGLLEKKGHTCHSRRKVSLHCVKEGKHKERRIMKGKTAIQGVQFWSKVESVQPARLAAATWLSSVPLCTQSREDADVWKVVFGINNLDHPSPFMQTRKVKRIIVHPRYNRAVVDYDISIVELDTEINETSYVRPVCLPHKGQPPLPDTYCYITGWGHMGNRMPFKLQEGEVRIISMDQCQSFFDMKTITSRMLCAGYESGTVDSCMGDSGGPLVCQEPDGRWSLYGLTSWGSVCFSKVLGPGVYSNVTHFVEWIERQIYLHTFFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2D1</accession>
    <name>W5N2D1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dehydrogenase/reductase SDR family member 6</fullName>
            <ecNumber evidence="10">1.1.1.104</ecNumber>
            <ecNumber evidence="11">1.1.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">(R)-beta-hydroxybutyrate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">3-hydroxybutyrate dehydrogenase type 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">4-oxo-L-proline reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Oxidoreductase UCPA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Short chain dehydrogenase/reductase family 15C member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20521"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Siderophore biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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    <dbReference type="EC" id="1.1.1.30" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01017798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017800">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2D1"/>
    <dbReference type="FunCoup" id="W5N2D1">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014790"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014819.1">
        <property type="protein sequence ID" value="ENSLOCP00000014790.1"/>
        <property type="gene ID" value="ENSLOCG00000012025.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156721"/>
    <dbReference type="InParanoid" id="W5N2D1"/>
    <dbReference type="OMA" id="YMTGTDF"/>
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        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012025">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016617">
        <property type="term" value="F:4-oxoproline reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:heme metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:lipid metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="DHRS6_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="249" mass="27095" checksum="FDFE5C5A4DB40B66" modified="2014-04-16" version="1">SSMGRLDGKLIVMSAAAQGIGRAAALAFAKEGAQVIATDINEENLKELDSYPVIGITTRVLDVTKKDQIEALAKELERIDVLFNVAGFVHHGTILDCEEGDWDFTMNLNVRSMYMMIKAFLPKMLAHKSGNIINMSSVASSIKGVVNRCVYSTSKAAVIGLTKSVAADFIEQGIRCNCICPGTVDTPSLRERIQARPDPEQALKDFLARQKMGRMCTAEEVAQLCVYLASDESTYVTGTEHIIDGGWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2D9</accession>
    <name>W5N2D9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tudor domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a central role during spermatogenesis by participating in the repression transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Required for the localization of Piwi proteins to the meiotic nuage. Involved in the piRNA metabolic process by ensuring the entry of correct transcripts into the normal piRNA pool and limiting the entry of cellular transcripts into the piRNA pathway. May act by allowing the recruitment of piRNA biogenesis or loading factors that ensure the correct entry of transcripts and piRNAs into Piwi proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TDRD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2D9"/>
    <dbReference type="FunCoup" id="W5N2D9">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014798"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014827.1">
        <property type="protein sequence ID" value="ENSLOCP00000014798.1"/>
        <property type="gene ID" value="ENSLOCG00000012030.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686945"/>
    <dbReference type="CTD" id="56165"/>
    <dbReference type="eggNOG" id="KOG2039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158754"/>
    <dbReference type="InParanoid" id="W5N2D9"/>
    <dbReference type="OMA" id="YCSAQKS"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012030">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034584">
        <property type="term" value="F:piRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20409">
        <property type="entry name" value="Tudor_TDRD1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000048">
        <property type="entry name" value="Tudor domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000011">
        <property type="entry name" value="Tudor domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047377">
        <property type="entry name" value="Tudor_TDRD1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF4">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="124"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="16">
        <location>
            <begin position="258"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="16">
        <location>
            <begin position="488"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="16">
        <location>
            <begin position="702"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="16">
        <location>
            <begin position="917"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1116" mass="124372" checksum="EF869CD73A798781" modified="2014-04-16" version="1">MEGDGKLFCGKELTTAVWPYPKMAEGCTRFEGSMSQPVVTPNLPLRRPTSSPIIKSSQLEVEIESSLTGGKNFQFSLASEEYLGNKSHGTAPREETNQNGNGSLSTEGISFTKSLGSPGTVKICHVCHQKGFMRCTRCKQTTYCSVSCQREDWKTHRHVCKPYSQKSSKNQQGQPTTTSEAKEMITIGKEQEKKMYLSDLQKMDIKKGLKVQACVVEFRNPGKFFIHIQTAKMIETLKKVTLTLQKIYSNSISEDDYIPDVEEVCAAQYSQDQNWYRCLVQSLDISSRTANVIYIDFGNEEDVKLDKIKRLSVDIDPVPPCALQCRVANLAAVTGSWVEVCCSSVRQLLLGKTCLVTIVDVSETDSVCSVDVALPSIGMQLEEFLVQQGYVVKQKVGKSSPNGQDINSILCAALENFRKKASTGVEDDKKFPEPVHLCIGDSFSCVVTHILSPHDFVIQKLDNAGAIQELQIRLRDHCVQIPSSEDYRPAPGTVCCSQFTEDNLWYRATVLGYSSEDRVFVGYIDFGNSEELELNRLRPANEEFLKLPMQAITCSLPGIKPVSEEWDSESILVMKKLVNTKFLKVRILDKVDVKALVELTDEYSDPQTNIADLMVAMRCAVIDLDFRSQTDQTEGTKESFTKKLEWTSTELPSNETVDVVVSVIDSPSEFYCQCCNPKDLQALNEMSANLLKCSQKASEAFTPVIGEPCCAVFPGDGNWYRGMINDIGPKGAEVYFVDYGNTSEVAVDKLCAILPEYLMLPFQAIKCWLAGVEPVQREWTKDAIKRFQALTVGVQLKAKVLCNGQNGYGIKLVKRGQNVAEKLVAEKLAVCNKTFHDPVLTSIMPGTEPTGGYFPKDWKTVELPKSGTFQLHIVAVRNPSLFYGLKDSNEVDVKKLEALMAELSSYCNKQSYNPNLKPEAGVACCAQFSGDKNWYRAIVLDICDSEAYVLYADYGNIEKVPLSSILPITAKHLELPFQIIKCQLSEELFPDEWSSISLECFESLLRGKVMASVRKFDGKIYTVHLTVCSDNGNVDVSKMMIEKFGKIKCETDAGVIVTDTNVKALLESNTTCTPPIDSQPTNVMKCCCQELKEKIDKLEELLLVVIKEIVHPQKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2Q9</accession>
    <name>W5N2Q9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibromodulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Keratan sulfate proteoglycan fibromodulin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds to type I and type II collagen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2Q9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014918"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014947.1">
        <property type="protein sequence ID" value="ENSLOCP00000014918.1"/>
        <property type="gene ID" value="ENSLOCG00000012131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688088"/>
    <dbReference type="KEGG" id="loc:102688088"/>
    <dbReference type="CTD" id="791822"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157007"/>
    <dbReference type="HOGENOM" id="CLU_000288_186_4_1"/>
    <dbReference type="InParanoid" id="W5N2Q9"/>
    <dbReference type="OMA" id="WWFQYLR"/>
    <dbReference type="OrthoDB" id="1668230at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012131">
        <property type="expression patterns" value="Expressed in larva and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000073">
        <property type="entry name" value="Lumican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF4">
        <property type="entry name" value="FIBROMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867166" description="Fibromodulin" evidence="15">
        <location>
            <begin position="19"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="16">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="353" mass="39652" checksum="2A3BB74C3F694023" modified="2014-04-16" version="1">MRLAALLLATGLYGLTLCQNQDNFFWLSYLRERWAGYNSFRGQDSVGSPGRECPLECDCLPSFPIAMYCDSRKLGHVPFVPSRMKYIYFQNNQITSIQDGVFDNATDLAWIMLHRNLLSTDNLGAKVFSNLLQLDRLYLDHNNLTRVPQGLPRSLRDLRLSNNRISKIQPASFEGMANLSVLLLNDNSIEEVGGALKGLTSLLLLDISNNKLKKLPDSLPEGLQQLYLEFNGIGSIPSDFFQRFAKLQYIRLSNNKLTDEGIPSNTFNFSSLIELDLSYNKLQKIPPVSTSLENLYLHANQIKEFSVSSFCSVIDIMNFSQLRVLRLDGNELAEGAVPAEAALCLRLAAIIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6R3</accession>
    <name>W5N6R3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Si:dkey-165a24.9</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6R3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016322"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016352.1">
        <property type="protein sequence ID" value="ENSLOCP00000016322.1"/>
        <property type="gene ID" value="ENSLOCG00000013250.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRUD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183136"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5N6R3"/>
    <dbReference type="OMA" id="NHERYKI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013250">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF9">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 132-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="34"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="323" mass="36944" checksum="1FD30933924EEBE1" modified="2014-04-16" version="1">AAEMAEDSCNITLAVDSVGLISIYSLVFALALPCNLLSLWGLYRLLRSGNVMPIFIFNLLVSDLLQVLTFPMWIIYLKNGHQWTFQAGACNFVGFLFYINLYASVAFLSLVALDRYLAIVHPLRSRGFRTVKVALVTSVIVWVVTFLFCLSGLYPTVYDEKKQLCLEKYPVAKRYAMFKIVTVILGFLVPCCILGYTSVRIRWELRSSPSVTQEERRKIVGILCIITVIFIVVFGPYHLVGSYKFVYYFITDSNCDMERTLFLCYRLCYGLTSLNSLLDPLLYIFICDNIRQELKKSLPCLRAYKMRKESQVQLNSNVEIASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6Y4</accession>
    <name>W5N6Y4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Monocarboxylate transporter 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Solute carrier family 16 member 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-linked monocarboxylate transporter. May catalyze the transport of monocarboxylates across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6Y4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016423.1">
        <property type="protein sequence ID" value="ENSLOCP00000016393.1"/>
        <property type="gene ID" value="ENSLOCG00000013302.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159372"/>
    <dbReference type="HOGENOM" id="CLU_001265_59_1_1"/>
    <dbReference type="InParanoid" id="W5N6Y4"/>
    <dbReference type="OMA" id="DLVWQIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013302">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17423">
        <property type="entry name" value="MFS_MCT11_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000163">
        <property type="entry name" value="Putative monocarboxylate transporter 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_MCT_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF19">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="18"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="46741" checksum="8A5870AB921CEC57" modified="2014-04-16" version="1">RGMPSAVRPQAPPDGGWGWVVVGALFVVSALVFGVIRSFGVFFVEFVDYFDERAGTVSWITSIGVSVQQLASPVATALCNAYGPRLVVMVGGLLSGLGMILASLSTTLVHLYLSVGIVSGMGWAMVFTPTVASVMQYFERRRSLAMALGFTGVGLSSFAFSPLFQLLVERYTWRGGLLILGGLCLNMVACGALIRPLRLREEGGEGGPERRASLPGGCLSQARAYLELSLLRHRPFLCYSLAVTLFNAGYFVPYVHLVAHSRQVGFSEYQAAFVVSATGVTDLVGRVVSGWVSDLGRLRLPHILALWTGLTGLSMAAIPLGTTYPALLTISLLYGFCAGAMTPVVFSVVPEIVGMERLFGALGLLQMIESVGGLLGPPLSGFLKDVTGSYVISFLVAGGFLLAGTLIMGTLPQFFSCANPPSQGPEPGSQANGTAHGKTEPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8A2</accession>
    <name>W5N8A2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centrosomal protein 112</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8A2"/>
    <dbReference type="FunCoup" id="W5N8A2">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016861"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016891.1">
        <property type="protein sequence ID" value="ENSLOCP00000016861.1"/>
        <property type="gene ID" value="ENSLOCG00000013670.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQAE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006544"/>
    <dbReference type="HOGENOM" id="CLU_012527_0_0_1"/>
    <dbReference type="InParanoid" id="W5N8A2"/>
    <dbReference type="OMA" id="RMHEKEX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013670">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055310">
        <property type="entry name" value="CEP112"/>
    </dbReference>
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        <property type="entry name" value="DUF4485"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18871:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 112 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14846">
        <property type="entry name" value="DUF4485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4485" evidence="3">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
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            <begin position="149"/>
            <end position="200"/>
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            <end position="847"/>
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            <begin position="918"/>
            <end position="945"/>
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            <begin position="163"/>
            <end position="173"/>
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    </feature>
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        <location>
            <begin position="175"/>
            <end position="190"/>
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    </feature>
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        <location>
            <begin position="812"/>
            <end position="828"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="953" mass="111219" checksum="2CBB4678AA171211" modified="2014-04-16" version="1">AVMSSQEDTWEKLDSEFDHYLVDMKPYVLKLPHTSERQRCALWIKKLCDPSGSGAGIMGRKNRNLYARLLLHMLRRGVLEGPFTHRPEPGSLKTLPTYMSIYFDEPLCGRPLEQGSVRLPDWVTGELGPNEVKASFSWSLNNDDITLQSPCQRRRRHTDSEKLTSQARSSVVTGRSDEENHVAFQTDDRHRKPVTSSDDSDLEARLNSWNLGIENPRYLREKPIPLSPISLKASLGKNSTFCDDQTSVHTHEKELEMKTKVLEARCHEEKLKMQQKHDADVQKILDRKNNEIEELKTLYRNKQKESEETIRKLEKKVQSLVRESQVIRESKEQQIAELKKISDQSADSLKNEWEKKLHNAVAEMEQEKFELQKKHTENIQELLDDTNARLAKMEAEYGAQTQATDQMVKEQEARVQQLTVEVESSNALRQKVAQEKAELEIQVAGISAELQEACRRNNSLLREKDQLSEQHEQSVRRMQTKRDADLSHLQQEHALSAAKASEVIEELEKTVTQLKQELRDTEHRRQKQLREQESKFHQERSNLEHIHDKKVRALQSEADRDKADATKKMGKLEEALREKEEQLGRLAGQQKLQAQQAEQALEQFRRQVELSSEKAYSDMKQQMEKVEADLARSKCLREKQSKEFTWQLEELKQRYEQQIVELKLEHEQEKTHLFQQHNAEKDSLVQDHEREIENLEKQSRAAMLQHESQTQEWRKRDTQAAAALEAQVQQLREELVRAGAQRKQQLLELSLQREEERQRAAQEQEAALGRLRAETDRARLDLERAHAAERQAGLEQTNSRLKQIEKEYSQKLAKSSQTITELQTSLSTAREESRQRQRAAESQLQETAARWEEERRQLLRDHEREIRALQDKIENLQSLLNSSEKKLLNKELEHQEQVTRVRQECEMKIKGLMPAALRQELEDTIASLKSRVNFLQKRAVVLQEDLDACRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9Z1</accession>
    <name>W5N9Z1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9Z1"/>
    <dbReference type="FunCoup" id="W5N9Z1">
        <property type="interactions" value="1807"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017450"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017481.1">
        <property type="protein sequence ID" value="ENSLOCP00000017450.1"/>
        <property type="gene ID" value="ENSLOCG00000014165.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007015"/>
    <dbReference type="HOGENOM" id="CLU_033458_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9Z1"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014165">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000795">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="36243" checksum="F85E7DBD76400851" modified="2014-04-16" version="1">MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKEGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDEKYKRPGYGAYDVFKQAVSDPSILDGLDLTEEERNVLIDNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTEAMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAAIKEKIEEKRGVFNIQMEPKVVTDTDETELARQLERLERENAEVDGDDDTEEMEAKTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAK9</accession>
    <name>W5NAK9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RAS and EF-hand domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199261.1">
        <property type="nucleotide sequence ID" value="XM_015343775.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAK9"/>
    <dbReference type="FunCoup" id="W5NAK9">
        <property type="interactions" value="830"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017668"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017700.1">
        <property type="protein sequence ID" value="ENSLOCP00000017668.1"/>
        <property type="gene ID" value="ENSLOCG00000014347.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687212"/>
    <dbReference type="KEGG" id="loc:102687212"/>
    <dbReference type="CTD" id="572256"/>
    <dbReference type="eggNOG" id="KOG0078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165251"/>
    <dbReference type="HOGENOM" id="CLU_023178_1_0_1"/>
    <dbReference type="InParanoid" id="W5NAK9"/>
    <dbReference type="OMA" id="RMVVEYQ"/>
    <dbReference type="OrthoDB" id="9879408at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014347">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001348">
        <property type="entry name" value="Ras and EF-hand domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="165"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="684" mass="77606" checksum="39AD3EF4FE5F00D9" modified="2014-04-16" version="1">MYILMDSPLADTDSQPDLQRLFSACDLNKSGRIEYEDFAAVCRELNVRSSEVAFLFNKFDVDQDGCIDYSDFSARFHEVSETLDLAALGGAPHEPSSPWEDFEDRMGDEIGYLGRGQEQVARLYQQIHAFSDDALLQQYESLIDTLVTEHRGHSLETEQLESALRRAEEMTAQQLTELEEDLQQQLASVEKRVREEERQRLEAAVAEIQRRHESEVADLQVAVERLRKQQEEFWHSSSREDIHQLKDQITEMTQENEQLRSSLVKAQMSVSILQVEMDKLKNEYADQKLQHEREKEVLRKMLAERQSYCNQIEILQTMNKKLYDSNDGLRSALVTSVGLGKKRHLSIRDESPLLSLKPGDQRMLNYSSFASEDDAMTVDSYEPTDTRYSQVASWADRYLDSGVSVSLEPGDESGSEYESDDSPNSVETVHYSYSYVQSDVEMSDLKSEGTASVAPSRRSSIASSIRRRLPAFTPKPMDTVDAEAPGPMYRLVLAGDAGAGKSSFLLRLSTNEFRGDIQTTLGVDFQMKKMLVDGEQTTLQIWDTAGQERFRSIARSYFRKAHGILLLYDVTSERSFLHVREWIDQVQDSTVDHIPMCLIGNKTDLRTEETESSCVHTAHGEKLAMAYNALFCETSAKEGTNVVEAVLHLAREVKKSTVLRRKSEPQMNVTIVDGKKALSSCCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBA1</accession>
    <name>W5NBA1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Estrogen-related receptor gamma-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627644.1">
        <property type="nucleotide sequence ID" value="XM_006627581.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBA1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017910"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017942.1">
        <property type="protein sequence ID" value="ENSLOCP00000017910.1"/>
        <property type="gene ID" value="ENSLOCG00000014553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698328"/>
    <dbReference type="KEGG" id="loc:102698328"/>
    <dbReference type="CTD" id="407692"/>
    <dbReference type="eggNOG" id="KOG3575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167036"/>
    <dbReference type="HOGENOM" id="CLU_007368_11_0_1"/>
    <dbReference type="InParanoid" id="W5NBA1"/>
    <dbReference type="OMA" id="RYRAMKM"/>
    <dbReference type="OrthoDB" id="5771769at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014553">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0034056">
        <property type="term" value="F:estrogen response element binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="NR_DBD_ERR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD_ERR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="estrogen-related receptor gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="131"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="239"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="50866" checksum="050473F637219D4C" modified="2014-04-16" version="1">MDLKDFCLSDSFHIFNQHSHLLSRSPIGDSPSSCEPSVKTEPPSPTLALDSATQFSPGGSSDSSGYSLLSQAHSLDPDSPSSCSAGSGGGGRALPDTCSAQFYRADRALSLPAADTLLKSDYAAALSSGPKRLCLVCGDFASGYHYGVASCEACKAFFKRTIQGNIEYSCPVMNECEITKRRRKSCQACRFRKCLQVGMMKEGVRMDRVRGGRQKYKRRVDSGGPFCIKAPYSSPTKPTGNKVISHLLMMEPTPVCAMPDPTTPDSDIKTLMTLCDLLNRELLVVIGWAKHIPGFSALSLVDQMALLQSGWMEALILVVVSRSLSFTEELVFAGNFRLDRAQCRAAGLQDLYTALKHLISKYQQMHLSPEEVVTLKAMVLANSDSGSVENPEAIQQFQDVLHEALQDFETGRHPGDPHRTGKLLMTLPLLRQTANRAVDTFCRLQLEGRVPMHKLFLEMLEAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBR2</accession>
    <name>W5NBR2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Low density lipoprotein receptor-related protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="7918.ENSLOCP00000018071"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018103.1">
        <property type="protein sequence ID" value="ENSLOCP00000018071.1"/>
        <property type="gene ID" value="ENSLOCG00000014674.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1215">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158287"/>
    <dbReference type="HOGENOM" id="CLU_000085_4_0_1"/>
    <dbReference type="InParanoid" id="W5NBR2"/>
    <dbReference type="OMA" id="NNRYTAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014674">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBZ4</accession>
    <name>W5NBZ4_LEPOC</name>
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        <recommendedName>
            <fullName evidence="7">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.3.8.1</ecNumber>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
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            <dbReference type="ChEBI" id="CHEBI:57389"/>
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            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21 22">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the acyl-CoA dehydrogenase family.</text>
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        <property type="reaction ID" value="UER00860"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_DH_CS"/>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
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        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
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        <property type="entry name" value="IVD"/>
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    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
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        <property type="entry name" value="ACYL_COA_DH_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 22" id="KW-0274">FAD</keyword>
    <keyword evidence="8 22" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="25">
        <location>
            <begin position="70"/>
            <end position="184"/>
        </location>
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        <location>
            <begin position="188"/>
            <end position="285"/>
        </location>
    </feature>
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        <location>
            <begin position="297"/>
            <end position="444"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="310"/>
        </location>
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        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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            <position position="198"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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            <begin position="222"/>
            <end position="224"/>
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            <name>FAD</name>
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            <begin position="308"/>
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            <name>substrate</name>
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            <position position="336"/>
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        </ligand>
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        <location>
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            <end position="408"/>
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        <location>
            <begin position="431"/>
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            <dbReference type="RuleBase" id="RU362125"/>
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        <source>
            <dbReference type="Pfam" id="PF00441"/>
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        <source>
            <dbReference type="Pfam" id="PF02770"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02771"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018153.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="450" mass="49212" checksum="F1AC2B9B2AB1C9B3" modified="2014-04-16" version="1">MRRLRIAVGGSVHARTSFTTLGKTLRYTGSSLTMLAARRALLSGYRSAAVWRRGCAGSAIPIDDIVNGLTDEQIQLRQTLQKFCQDHLALKAQEIDKNNEFKEMREFWKKLGDLGVLGITAPLELGGSGLGYLDHVIVMEEISRASAAVGLSYGAHSNLCVNQLVRNGNQAQKEKYLPKLLTGEHIGALAMSEPGAGSDVVSMKLKAEKKGDYYVLNGNKFWITNGPEADVLIVYAKTEPKAQPVARGITAFIVEKEMPGFSTAQKLDKLGMRGSSTCELIFEDCEVPEENVLGHVNKGVYVLMSGLDLERLVLAAGPLGIMQAVLDHSIPYMHVREAFGQKIGHFQLLQGKMADMYTRLSSSRQYVYNVARACDKGHFNAKDCAGVILHAAETATQVALDGIQCLGGNGYINDYPMGRFLRDAKLYEIGAGTSEVRRLIIGRAFNAMYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC17</accession>
    <name>W5NC17_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Extracellular tyrosine-protein kinase PKDCC</fullName>
            <ecNumber evidence="3">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Protein kinase domain-containing protein, cytoplasmic</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Protein kinase-like protein SgK493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Sugen kinase 493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Vertebrate lonesome kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:10597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="W5NC17"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018176"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018208.1">
        <property type="protein sequence ID" value="ENSLOCP00000018176.1"/>
        <property type="gene ID" value="ENSLOCG00000014760.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697009"/>
    <dbReference type="KEGG" id="loc:102697009"/>
    <dbReference type="CTD" id="565254"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001205"/>
    <dbReference type="HOGENOM" id="CLU_044025_1_0_1"/>
    <dbReference type="InParanoid" id="W5NC17"/>
    <dbReference type="OMA" id="GSIRMNK"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014760">
        <property type="expression patterns" value="Expressed in ovary and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="extracellular tyrosine-protein kinase PKDCC isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042983">
        <property type="entry name" value="PKDCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448:SF2">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 25" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869527" description="Extracellular tyrosine-protein kinase PKDCC" evidence="25">
        <location>
            <begin position="22"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="125"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="485" mass="54233" checksum="B0E5706CB7AD1FFA" modified="2014-04-16" version="1">MYSLSIPFCAAALTVFLFTASRFFSDSRHLHVDEGDSVSGASGSAPVSQQSPILKEIKEKHRELLPYYTESTKETFLKVQWKERGDGDSDSLTNHGLARTKVKDILDVEESAGTGMIGCVQLRYFKNVDFLGSGYTKAVLKAVSPEGLPVALKSVNDRGTDMKRCLEDFGDVEGCYELVSYKLIKEIMLLQRLQHPNIIKLQGHCQGRDWGSGVTAALELGSPLQMIQLLQSPWEERFRICLSLVRLLHYLSISPLGSVALLDFQPRQFVVVSGELKLTDLDDASTEERSCRTDSDCTLTFPHRNFTFPCAAGGICEGLNEKTNLYNAYRYFFTYLLPHRAPPTLQHLIDNIMNSTGELRSGINETLEGFEEILQMYKSGRYLNNLPPSLLRGYTLVKGKRPVDSVDYRCWPSYNHQGCVLSVHSAREAAHICSSHPQCTSFSVSSQRTWTGRYLASFRSGFRDLIPDVKSALYVKKVCGSEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC43</accession>
    <name>W5NC43_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Interactor of little elongation complex ELL subunit 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC43"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018202"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018234.1">
        <property type="protein sequence ID" value="ENSLOCP00000018202.1"/>
        <property type="gene ID" value="ENSLOCG00000014783.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUWA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006883"/>
    <dbReference type="InParanoid" id="W5NC43"/>
    <dbReference type="OMA" id="LPFHQQH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014783">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019535">
        <property type="entry name" value="ICE2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633:SF3">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10505">
        <property type="entry name" value="NARG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Little elongation complex subunit 2 C-terminal" evidence="2">
        <location>
            <begin position="687"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="909" mass="100600" checksum="91D7EE4B75D90CA3" modified="2014-04-16" version="1">MELNWDVAPKNGTTVFFSREVYDNYSLAPNIKELWAVALSPPVKAKEATKEQKCEANDLQPKSTSSVQEEKAPTSAPADTKDVFPEPKVPYPCLSGLTQVEQKMYIHMLTAKSNISEITIMNFIVFSTFEICLFQRLQELVQIEVPEYMKFLQNAARICKDDYNSISEGAARYSEEYLRACSEKVKNYPEVYVIHEMTSMTGGKFSQELSLNFEKHLLSLGQVKMFKAKPLLNAKGIQMATDYESVAAEFPPEKKACIAHTDISSDMNAESLCMKYSPHVSMTSQALYTLLNNHGPDYNDSWELPLCVKKISGEGESVKKVVFIDSPLLKREITVREKNQIFHKESIHLLLKSGSLKQVSEVLLNNTTLDPVVPWTDRRPVSFDDMEVTFEADFTDLETFGESSSSSKMSNSKSTSKNLKTTASCKTQEGSQSSDATKQLGKSPATTSEDLKSPDSKLDFKGSARSPVMQQKQVSKANLFSSDADSDDERLVIDDPLARARSVKGDESLSLASASAERPAQVPEPVPDTPRSPSPEHGRESLASTGTKGIRRALKRPKTGVSKDCDQLGQILKMQCALLKPSSKATQEPMSPQANTNSLPATAQSHARPLVKACVSSFLEASQRTTGAALAAPAAPHADCPQALPPQKHNTVCKILALVLMYLLRLRVFCLTGLLQGSLSAVEEDDQEYRAPQEGNLLYQLYSLGDMLLLVRSTIDCSFIVQCGDCKTQKRCFVFPVYVLPKLEYQLGHGVEILTKSEACRLWVEKLLHSNTMFYIGHIDALTSKLFMLDEFLPDSSTNAFVDFKPANMLNILHHLLNKILGLQEGRYLLSHRAGEALVTISRVCDGMKVTRATLNLHEAHSSPPRAPSSGVVPWVPVDSSRVLDFHVKQGRVPCTFPPRPAAKKQGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDT1</accession>
    <name>W5NDT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydrofolate reductase</fullName>
            <ecNumber evidence="3">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01025403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDT1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018790"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018822.1">
        <property type="protein sequence ID" value="ENSLOCP00000018790.1"/>
        <property type="gene ID" value="ENSLOCG00000015270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694946"/>
    <dbReference type="KEGG" id="loc:102694946"/>
    <dbReference type="eggNOG" id="KOG1324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165780"/>
    <dbReference type="HOGENOM" id="CLU_043966_2_3_1"/>
    <dbReference type="InParanoid" id="W5NDT1"/>
    <dbReference type="OMA" id="VMADFDC"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015270">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.430.10:FF:000002">
        <property type="entry name" value="Dihydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF5">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="12"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="195" mass="22316" checksum="514A6CF91257FCC2" modified="2014-04-16" version="1">MSDCEGKVHRKSIRLIAAACNGMGIGKDGILPWSLPKEFKFFLDTITSVSSPDKKNLLIWGKRCWISFPESLHPLANCIHVVLSRTMGCVPDHAHYLCHDLPSVIQLGSTHPLSDKIETIWILGGAELYKESLKHPWCDYIYLTQVMADFDCDTFFPEFDREIYKLQDEFPGVPSEIQEDQGIKLKFQVFKKDMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDT9</accession>
    <name>W5NDT9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Polymerase (DNA directed), mu</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDT9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018798"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018830.1">
        <property type="protein sequence ID" value="ENSLOCP00000018798.1"/>
        <property type="gene ID" value="ENSLOCG00000015276.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158490"/>
    <dbReference type="HOGENOM" id="CLU_365209_0_0_1"/>
    <dbReference type="InParanoid" id="W5NDT9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015276">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17713">
        <property type="entry name" value="BRCT_polymerase_mu_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000032">
        <property type="entry name" value="DNA-directed DNA/RNA polymerase mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000035">
        <property type="entry name" value="DNA-directed DNA/RNA polymerase mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027249">
        <property type="entry name" value="DNA/RNApol_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019843">
        <property type="entry name" value="DNA_pol-X_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001726">
        <property type="entry name" value="TdT/Mu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF24">
        <property type="entry name" value="DNA-DIRECTED DNA_RNA POLYMERASE MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000817">
        <property type="entry name" value="DNA_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501176">
        <property type="entry name" value="DNApol_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00871">
        <property type="entry name" value="DNAPOLXTDT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00522">
        <property type="entry name" value="DNA_POLYMERASE_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="9">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="601"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="603"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="561"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000817-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="764" mass="85309" checksum="3F58C94086F2850E" modified="2014-04-16" version="1">MVPLKRRKTVRNDVNCERNEVIKFPDVVIFLLERRMGASRRAFLTRLGRNKGFRVEDCYSDAVTHVVSENNTGEEVVDWLDRQIPGGWTPRPVHLLDISWFTESMGAARPLDVQDAHRLKVKAVSQAGGGTRTVSPYACGRRTPLQHHNRALTDALEVLAENERFRKPPGSSVATGRPDVLYRPLGFKLGCTPPPRCGKHSQQNRVLWIALQSSQRRLSLYTDYTEAFQLLRLPPFHFSLIKCTTDNLTQRGLQVLALRTRSQREFSSGQGTGVRHYEDLSTPITKEEAHAIGQIVEEAVHTVLPGAELTITGGFRRGKKTGHDVDFLITHPEEGKEVGLLPKVISWLDSQDLLLYHRVKDNTYSESKVQLARSQSSMDHFERCFSIFRLNRPLARPPEPGASSGGTAQESAASGGHCSGEPRSWKAVRVDLVVTPVSQFAFGLLGWTGSQHFERELRRWAGQEKHMTLNSHALYDRTQGSHRAKDHHQTIATSMASSPPLSLSRWLSPLHHRAHTRERYCEPSAPPTESMAIASHEKGEIQAHMPVEYSRGGKRISLHTLEPEIASGTEEEETSGYESEGGQSASPADPPGGSSSSPPTPPGRRPRTAFTSEQISRLERTFKKHAYLGTREKEELCRKLNLSEKQIKNWFQNRRMKLKRTLQDALAQACHVKVASQLLHYPELQAFGPSAYSGYYPNQDSTAAYLPLPGLPYAPSQALGHLSALPLEAQVHPYSVPPFVMPPHSAGTGSPPVMARYHPYAPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF06</accession>
    <name>W5NF06_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Centrosomal protein 41</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CEP41 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633338.1">
        <property type="nucleotide sequence ID" value="XM_006633275.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF06"/>
    <dbReference type="FunCoup" id="W5NF06">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019215"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019247.1">
        <property type="protein sequence ID" value="ENSLOCP00000019215.1"/>
        <property type="gene ID" value="ENSLOCG00000015609.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685865"/>
    <dbReference type="KEGG" id="loc:102685865"/>
    <dbReference type="CTD" id="95681"/>
    <dbReference type="eggNOG" id="ENOG502QR8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002222"/>
    <dbReference type="HOGENOM" id="CLU_064316_0_0_1"/>
    <dbReference type="InParanoid" id="W5NF06"/>
    <dbReference type="OMA" id="TMCQRGF"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015609">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000012">
        <property type="entry name" value="Centrosomal protein of 41 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051889">
        <property type="entry name" value="CEP41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44390">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 41 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44390:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 41 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rhodanese" evidence="12">
        <location>
            <begin position="173"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="329"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="41660" checksum="939BD533A713DDD4" modified="2014-04-16" version="1">MSARRGFGNSEYLQKKIPQNPKYQHVKSRLDTGCSLTKYMERLEEIKKNYRYRKDELFKRLKVATFAQLVIQVASVSDHDDENDKDVHSLDDNISVMSETDAELLSERTNGTPETTPVQFIDNNNEVGDTGISPRSTLQSVISGVGEMDLDKTAQKNVHQKPSSPMMTDKPYPDCPYLLLDVRDRDDYDRCHVIGAYSYPIATLSRTMNPYTKEVLEYKNASGKIIILYDEDERIASQAATTMCERGFENLFMLSGGLKVIAQKFPEGMTTGSFPAQCLQSGPPTSGRKRSAPRQPSYPAEDKWRFTAEDLNKMQFYLEEMLIPADTNSRLSRLSSGSSSAKTFCSSARSRQTPSVAGSGSARSQSSRPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF18</accession>
    <name>W5NF18_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF18"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019227"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019259.1">
        <property type="protein sequence ID" value="ENSLOCP00000019227.1"/>
        <property type="gene ID" value="ENSLOCG00000015618.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2646">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001878"/>
    <dbReference type="HOGENOM" id="CLU_050434_0_0_1"/>
    <dbReference type="InParanoid" id="W5NF18"/>
    <dbReference type="OMA" id="LICHRAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015618">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048584">
        <property type="entry name" value="Ribosomal_uS5m_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21251">
        <property type="entry name" value="Ribosomal_uS5m_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867528" description="Small ribosomal subunit protein uS5m" evidence="9">
        <location>
            <begin position="18"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="182"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="31950" checksum="A8BACE13DDF37B07" modified="2014-04-16" version="1">MAAARWVWSALRIPVRGGILVPTQACTSAVQTTWAVSSTTASVSVQQYRFSSFFNKLTATELWRGVLAESGPGARKGRGKRTKRKLKKDLNRGQSVGEGRGGYLWPGLNSPVLRSGTIQSISQRDQAQQEELQADIVRQRDEWDRKRRTKVKRDRGWTGNSWGGISLGSPDPGPYGETYEDFDSRVIEVKSVFNMTANEGRKRSISALVAVGNGNGAAVGFALGKAADRATALRKAKNKAVHYLYYIERYNNHTIYHDMTSTFKRTTLRMKKQSRGKSGTASEGPVSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFN9</accession>
    <name>W5NFN9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFN9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019480.1">
        <property type="protein sequence ID" value="ENSLOCP00000019448.1"/>
        <property type="gene ID" value="ENSLOCG00000015797.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2782492_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFN9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015797">
        <property type="expression patterns" value="Expressed in heart and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="69" mass="7879" checksum="D4773CC287551E59" modified="2014-04-16" version="1">MISCRLDQKPSVIGGRGCAGKVLGNIAVGYKTQGFVYTVSLTYPLQSWSRRKYKFQNSSYWKMSRQRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFS4</accession>
    <name>W5NFS4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CCM2 scaffold protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_015201257.1">
        <property type="nucleotide sequence ID" value="XM_015345771.2"/>
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    <dbReference type="AlphaFoldDB" id="W5NFS4"/>
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        <property type="interactions" value="657"/>
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        <property type="protein sequence ID" value="ENSLOCP00000019483.1"/>
        <property type="gene ID" value="ENSLOCG00000015826.1"/>
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    <dbReference type="GeneID" id="102696234"/>
    <dbReference type="KEGG" id="loc:102696234"/>
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    <dbReference type="eggNOG" id="ENOG502QSZM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016168"/>
    <dbReference type="HOGENOM" id="CLU_034621_1_0_1"/>
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    <dbReference type="OrthoDB" id="5828470at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015826">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cardiac jelly development"/>
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        <property type="term" value="P:cell-cell junction assembly"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd13516">
        <property type="entry name" value="HHD_CCM2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PTB_CCM2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cerebral cavernous malformation 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CCM2_C"/>
    </dbReference>
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        <property type="entry name" value="Malcavernin"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642:SF4">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16545">
        <property type="entry name" value="CCM2_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="68"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="454" mass="49833" checksum="8CE752921F83872B" modified="2014-04-16" version="1">MEEDGKKAKKPGIVSPFKRVFLKGEKGRDKKAQEKVTERRALHTVPLSLPDHRVDPDILLNDYIEKEVKYLGQLTSVPGYLNPSSRTEVLHLIDNARKAHQLPGQLTAEQDAVVSLSAYNVKLVWRDGEDIILRVPIHDIAAVSYIRDDSLHLVVLKTAQEPGSSPCSSTCPEVSKSQTLGSLSESGAVLVEVCCLLVLAAENKAAAEELCLLLSQVFQIVYTESTIDFLDRAIFDGASTPTRHLSLYSDDSSSKVDVKEAFETEASTFSFQGSLDTGGNSPSSSSTPASPQPKTVSESELSTTAAELLQDYMMTLRTKLSSQEIQQFAMLLHEYRNGSSIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRYRRTMSTASNSTTNGNGAAGGSDDHLVPSEGDEWDRMISDISNDIEALGCSMDQDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH17</accession>
    <name>W5NH17_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01001660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH17"/>
    <dbReference type="FunCoup" id="W5NH17">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019926"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019959.1">
        <property type="protein sequence ID" value="ENSLOCP00000019926.1"/>
        <property type="gene ID" value="ENSLOCG00000016170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683958"/>
    <dbReference type="KEGG" id="loc:102683958"/>
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    <dbReference type="eggNOG" id="KOG0336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163653"/>
    <dbReference type="InParanoid" id="W5NH17"/>
    <dbReference type="OMA" id="RWAKCPP"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016170">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17958">
        <property type="entry name" value="DEADc_DDX43_DDX53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22430">
        <property type="entry name" value="KH-I_DDX43_DDX53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="292"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="479"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="648"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="677" mass="76885" checksum="23F3A8BDEC494C46" modified="2014-04-16" version="1">MSDWEADDDETQRVIKAEKREWKPVQSWKNSRFERKEGWGPPKPHGRGERVELQWQPSWQSSSDGANRAGSRFGERSKSQSTAALTFTIENSAIGRVIGRGGMKIRELQETTGTRIKVNRGEYQGEVQIYGRGDAQQKAKELIDELLSENSEDGSRYRRGSRDTCNSGRKELCWSKAAVQSTEVSAAPPIDWVSLRENRHRYEAMKWENLPPVKKEFYIEAESVTNLTAEEVEKWRKENNNIYVDDLKDGEKRPIPNPVRTFEEAFHHYPEIMENILQVGFQKPTPIQSQAWPVALKGIDLIAIAQTGTGKTLVYLLPGFIHMDQQPVSRKKRDGPGMLVLTPTRELALQIEAECAKYTYKGFTSICIYGGGDRKSQINMVTQGVDIIIATPGRLNDLQMNAYINLKSITYLVLDEADRMLDMGFEPQIMKIILDIRPDRQTIMTSATWPAGVRRLAKSYLKDPMMVYVGTLDLAAVNSVEQAVLIVQEEEKKDYVFDFIQKMGPEDKVLIFVGKKLVADDLSSDLCLHGIPVQSLHGDRDQCDREQALDDFKKGRVRILVATDLASRGLDVHDITHVFNYDFPRNLEEYVHRVGRTGRAGRSGASLTLVTRQEWKIASELVHILERANQAVPEELVLMAERYEKHQHEKELLGGSSRGAGGRRGREKQRANSSWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH24</accession>
    <name>W5NH24_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon gamma-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II (or gamma) interferon family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH24"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019933"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019966.1">
        <property type="protein sequence ID" value="ENSLOCP00000019933.1"/>
        <property type="gene ID" value="ENSLOCG00000016176.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248984"/>
    <dbReference type="InParanoid" id="W5NH24"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016176">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005133">
        <property type="term" value="F:type II interferon receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002069">
        <property type="entry name" value="Interferon_gamma"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11419">
        <property type="entry name" value="INTERFERON GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11419:SF0">
        <property type="entry name" value="INTERFERON GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00714">
        <property type="entry name" value="IFN-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867749" evidence="7">
        <location>
            <begin position="20"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="149"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="187" mass="22218" checksum="14F3D9C70CD78BB2" modified="2014-04-16" version="1">MNSLQRLLLFCGLCAICFGNGNCNDLVPEDVKEDIRVLATHFVKKSSVENLCWNMFILFLIDITENQESEEKLLLNEILNLYIKIFDNMNNVEEDKTLAERIHHIKIRLSHLKNATFQETSTNLKQQLELLWNIKVNDPVVQRKAVFELNQVLQKAENVGRKMHEKKEKQRRRRQAKLLRRRMQNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH48</accession>
    <name>W5NH48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing regulator WTAP</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Female-lethal(2)D homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">WT1-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Wilms tumor 1-associating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fl(2)d family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203536.1">
        <property type="nucleotide sequence ID" value="XM_015348050.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203542.1">
        <property type="nucleotide sequence ID" value="XM_015348056.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH48"/>
    <dbReference type="SMR" id="W5NH48"/>
    <dbReference type="FunCoup" id="W5NH48">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019957"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019990.1">
        <property type="protein sequence ID" value="ENSLOCP00000019957.1"/>
        <property type="gene ID" value="ENSLOCG00000016191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693736"/>
    <dbReference type="CTD" id="9589"/>
    <dbReference type="eggNOG" id="KOG2991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013931"/>
    <dbReference type="HOGENOM" id="CLU_044551_1_0_1"/>
    <dbReference type="InParanoid" id="W5NH48"/>
    <dbReference type="OMA" id="NTDPHED"/>
    <dbReference type="OrthoDB" id="3366661at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016191">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033757">
        <property type="entry name" value="WTAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15217:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING REGULATOR WTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15217">
        <property type="entry name" value="WILMS' TUMOR 1-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17098">
        <property type="entry name" value="Wtap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="234"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="394" mass="43757" checksum="2DF1BFD500BE1EB9" modified="2014-04-16" version="1">MTNEEPLPKKVRLSESDLKTLTREELCVRWKQHEAYVQLLEAKYAELNSNDVTGLKESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLSQQGQQQCPAPGTSRTSSSESSGPAEAAGKDCGRVANGPSNGNPSPRTSGPSLYREVSSTEEDFPSSPSNETKLSNHSEERTSRGGSSGYMNQLSAGYESVDSPTGSETSLTHHSNDTDSNNDPQEEKSVSGKGNRTAGSRHAQNGLDTTVNAHGSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH59</accession>
    <name>W5NH59_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nothepsin</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1 5">Belongs to the peptidase A1 family.</text>
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            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
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            <begin position="17"/>
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            <dbReference type="RuleBase" id="RU000454"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHQ4</accession>
    <name>W5NHQ4_LEPOC</name>
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        <submittedName>
            <fullName evidence="9">Phosphatidylinositol glycan anchor biosynthesis, class F</fullName>
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    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000016331.1"/>
    </dbReference>
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            <end position="30"/>
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            <begin position="183"/>
            <end position="209"/>
        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="219" mass="24338" checksum="2DDEC866E0075A21" modified="2014-04-16" version="1">MKDIEIKGMISAHTIMAASVLMATVLPLLFVDGFSVYGTHLVWLYSVSGAVAAVNITVYWLLGISPPSKKNTLSYKVSKAFRSCLYFILSCFLFHTVVVLYGAPLLESALETFSFAVLLSTFTTLRCLCILGPNVQAWIRVFSRDGAMSVWDTSLQITTACSMIGAWLGAFPIPLDWDRPWQVWPVSCTLGATFGFLTGLVVAPLWIHWHRKQLTYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI18</accession>
    <name>W5NI18_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 38 member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01036299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI18"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020277"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020311.1">
        <property type="protein sequence ID" value="ENSLOCP00000020277.1"/>
        <property type="gene ID" value="ENSLOCG00000016419.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160716"/>
    <dbReference type="HOGENOM" id="CLU_009020_0_1_1"/>
    <dbReference type="InParanoid" id="W5NI18"/>
    <dbReference type="OMA" id="CSAMAIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016419">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006868">
        <property type="term" value="P:glutamine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF184">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID SYMPORTER 1"/>
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    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="332"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="375"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="445"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="466" mass="52088" checksum="D35034F36413E3DA" modified="2014-04-16" version="1">MKRDGYSAVKQSSDIQDNYGNEDGFPNDGNVEMDIGRDVEREQEQGDFESQTSLTKEHLKNKKYEECGSGRTSFGMSVFNLSNAIMGTGILGLSYALSKTGIFLFLILLLSVAVLSLYSIRLLLECSKKAECMVYEDLGHKAFGMPGKLAVFGSSCLQNIGATLSYMFIVKNELPKVIATLAGLKEDLDVWYLNGNYLVIIVTIGIILPLCLLKSLGYLGYTSGFSLTCMVFFLIVVISKKFTLPCTTEKQNVTNESYLETDHCTAEYFTFNNKSVYAVPTILFAFVCHPSVLPIYSELKERSIKKMEKVSIISFLAMLLMYLLTAVFGYFTFYGSVHEELLHTYSSEGDTIILAVRLAIIVAVVLTVPVLFFVVRSSMLMIIKQKQFKWSWHIGVTLVLLAFCVLLVIFIPSITDIFAIIGATSANMLIFILPASIYLKLHQSIPTKKKIKVCCACQWVLHWLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIA5</accession>
    <name>W5NIA5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Platelet-activating factor acetylhydrolase</fullName>
            <ecNumber evidence="4">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01009120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIA5"/>
    <dbReference type="FunCoup" id="W5NIA5">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020364"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020398.1">
        <property type="protein sequence ID" value="ENSLOCP00000020364.1"/>
        <property type="gene ID" value="ENSLOCG00000016485.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="loc:102697204"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005233"/>
    <dbReference type="HOGENOM" id="CLU_022501_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Platelet-activating factor acetylhydrolase"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
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    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="PAF_acetylhydro_eukaryote"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <position position="241"/>
        </location>
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            <position position="319"/>
        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="402" mass="45778" checksum="31A1F6B161BF1604" modified="2014-04-16" version="1">MGSIWSTSLRIPPGKGPNHVGCTDLMMDHTAQGAFFRLYYPCETPTGYEMPGWIPNKEYFNGLADFLKLNRCLSERIFNYLYGSFTIPTAWNAPFKPADKYPLIIFSHGLGAFRTLYSAICIEIASRGFIVAAVEHRDESSSATFYFKEKSNRTEQEQLVISPNNFEEEWIYYRTLKQGEPEFPLRNKQVQQRANECIKALNLLTNINAGEPVTNALQLQFDWSRLKGSMDLCKTAVMGHSFGGATVIESLCKEPKFKCGVALDAWMFPLDDEVYARVNKPIMFINSEKFQWAGNIIKMKKLDSPTIQRKMITIRGSVHQSFPDFTFLTGNWIGKLLKLKGEIDPEVALDLSNKTSLAFLQRHLVLEKDFNQWDPLIDGKDDNLIAGTNVHLPPSMEMEEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIW6</accession>
    <name>W5NIW6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ADP-dependent glucokinase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027748">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626130.1">
        <property type="nucleotide sequence ID" value="XM_006626067.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIW6"/>
    <dbReference type="FunCoup" id="W5NIW6">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020575"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020610.1">
        <property type="protein sequence ID" value="ENSLOCP00000020575.1"/>
        <property type="gene ID" value="ENSLOCG00000016655.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692797"/>
    <dbReference type="KEGG" id="loc:102692797"/>
    <dbReference type="CTD" id="566398"/>
    <dbReference type="eggNOG" id="KOG4184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017953"/>
    <dbReference type="HOGENOM" id="CLU_032362_0_0_1"/>
    <dbReference type="InParanoid" id="W5NIW6"/>
    <dbReference type="OMA" id="YQICVAP"/>
    <dbReference type="OrthoDB" id="5847021at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016655">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043843">
        <property type="term" value="F:ADP-specific glucokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF1">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="485" mass="53331" checksum="FE525017E231AA76" modified="2014-04-16" version="1">MATDTGSSLSVVKFGTIVSLTVVLLAFWYRSPVQDTLDERLDTVLSSLLRAESKVGIDNIRRPRVAIGFGGCVDIIVDGVALLNKVGIQPSSTPLHYNFIENVEQLSRSFSYFFPPGAASERFVVNETLFSELVEASRELPGNRWAVGGNAAVMATRLANEGSEVLLGGSFSPEFTDVLPPHITVAGKTLEEPDIHLILEYPSGAKWGQYTSRRANRYIIHSDNHNPLLDSMEDFEEQLQTFGPDLLVLGGLQMMDNFPFQKGEREARLERLQRLMESANLGTGVHFEMASFVDKGLLEELLDRVLPYADSLGMNEQELPNLLSLMKGGNVTVLSDPNPRVATVLDQMRELYRLVNAQHSRQGRGRALTRLHVHTLAFQAIIVTKGSSWKNTMSATAKASLTANRHVCGSPDINPSKAKLIMDDSFSISRKEGSQRMPFQETKPVSCWDEADYEICVAPVLVCTEVYQTAGGGDNISAAGLVLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJE9</accession>
    <name>W5NJE9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 30 member 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJE9"/>
    <dbReference type="FunCoup" id="W5NJE9">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020758"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020794.1">
        <property type="protein sequence ID" value="ENSLOCP00000020758.1"/>
        <property type="gene ID" value="ENSLOCG00000016795.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1483">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159967"/>
    <dbReference type="HOGENOM" id="CLU_013430_4_3_1"/>
    <dbReference type="InParanoid" id="W5NJE9"/>
    <dbReference type="OMA" id="WARSEIL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016795">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055071">
        <property type="term" value="P:manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF3">
        <property type="entry name" value="CALCIUM_MANGANESE ANTIPORTER SLC30A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="11"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="35235" checksum="F361B75B7B1313A9" modified="2014-04-16" version="1">MGRYSGKTCRLIFMLVLTFVFFVAEIVAGYLGNSIALVSDSFNMLSDLMSLCVGLTAARVSRRPSSGRCTYGLLRAEVVGALANAVFLGALCFSITVEAVKRLVSPERIDDPKLVLIVGALGLAVNIVGLLIFQDCGGCARRKRKRGSSAREKEDAHGGAGAPVLTGSTPDQEGNVKEDSQPLNIRGVLLHVLGDALGSVVVVVASAIFFALPLPPEAPCNWQCYVDPSLTIVMVIIILSSAAPLVKETGSILLQMVPQGLAVHGIGEPPAPHWGAVFSAPRRPAHTCRPIGPVLAHKQHETKFKDNKYCFHRKAHDLCRWYSFHTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJL3</accession>
    <name>W5NJL3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AT-rich interactive domain 1B</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011768">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020822"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020859.1">
        <property type="protein sequence ID" value="ENSLOCP00000020822.1"/>
        <property type="gene ID" value="ENSLOCG00000016845.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155634"/>
    <dbReference type="HOGENOM" id="CLU_000974_1_0_1"/>
    <dbReference type="InParanoid" id="W5NJL3"/>
    <dbReference type="OMA" id="MWIDRYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016845">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035060">
        <property type="term" value="C:brahma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035176">
        <property type="term" value="P:social behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16877">
        <property type="entry name" value="ARID_ARID1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038040">
        <property type="entry name" value="ARID_ARID1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021906">
        <property type="entry name" value="BAF250/Osa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033388">
        <property type="entry name" value="BAF250_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656:SF11">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656">
        <property type="entry name" value="BRG-1 ASSOCIATED FACTOR 250 BAF250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12031">
        <property type="entry name" value="BAF250_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARID" evidence="5">
        <location>
            <begin position="937"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="850"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="900"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1260"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1596"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1734"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="72"/>
            <end position="94"/>
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    </feature>
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        <location>
            <begin position="107"/>
            <end position="118"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="141"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="321"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="409"/>
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            <begin position="458"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="502"/>
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        <location>
            <begin position="519"/>
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        <location>
            <begin position="563"/>
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        <location>
            <begin position="610"/>
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        <location>
            <begin position="635"/>
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        <location>
            <begin position="701"/>
            <end position="710"/>
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        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
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        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
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        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
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        <location>
            <begin position="819"/>
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        <location>
            <begin position="853"/>
            <end position="871"/>
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            <begin position="872"/>
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            <begin position="1057"/>
            <end position="1073"/>
        </location>
    </feature>
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        <location>
            <begin position="1095"/>
            <end position="1106"/>
        </location>
    </feature>
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        <location>
            <begin position="1266"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1303"/>
            <end position="1318"/>
        </location>
    </feature>
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        <location>
            <begin position="1319"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1400"/>
            <end position="1413"/>
        </location>
    </feature>
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        <location>
            <begin position="1613"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1644"/>
            <end position="1655"/>
        </location>
    </feature>
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        <location>
            <begin position="1672"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1751"/>
            <end position="1761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2148" mass="229370" checksum="27459EEE78870DEA" modified="2014-04-16" version="1">MANTTSSSANNTSDLREKEGVNSAASLSSSNSATSSSMETGLIANHKLKNVGGGDPPPPSHHHTPTQPQPPRFSQFQQLHSHQIQNNNINNNSNSGGGNQAQVPGEAENRQHGGKEHILGNQVGQQQPLLNKSEEEGRACKSGDQMGSRYEHANLGPANNNNSSSNSNNSSQPQSAGGTTSTVPEFNNYYGNARGGPCFDQHGGQQSPGMGIMHPSAPNSMDSVQNSHEGYHNSQYNHYPSYRPGYGGAGYGIMSSPRQGSHTLMGPGSNSHSSAAGHGKIAMAAASAPGGSVGGFQRFPGQSQHPSGATPTLNQLLTSPSPMMRGFSGGGYPEYSGPAAPPQQQASVGLGKDASSQYGSTAHGWGGHQRNHPVMSPGNTGQVISRPQAGAVDSMAMKRSQLYGVGSNPYSQQPQQQQQQPPPQQSGTYPGQPYGSPTAHRYPMGMPGRSQAAMGGVQYPQSQQMPPQYSQQGMSGYCQQGQPPYFSQPQPPSALTQPAYMQPRAPAQQLPPQEVPQESYGNRSQPSMTPGKPSHEDMNLIQQDRPSSLPDLSGSIDDLPTGTEAALSSAVSASGSTSSQGEQSNPAQSPFSPHASPHLSGMRGGPSPSPVGSPGGSNQSRSGPISPASIPGSQMAPQTPGNLSDAGSHSTMSQSPMSQERGFMGGMQRNPPVPPYGPQGSGPPMSPHPSPGAQMHHGLGSYQQGSSSNTYGPQSGQYGPQGNYPRPPSYGGTPSANYSGPGPGLSNSLGMNASSPMHGQGPGQPCGSMPMGRAPGSGMSGRPYSGGMSNMAPSSPSMPQPTGPGMGPPLPSVNRKAQEAAAAVMQAAANSAQSRQSNYSGMNQMGMMGSSSPYSQTMGNSSGMMTSQGPPYNTPPSGINPPGPMSVAGDMMMAGDTKLKGDTKEESIPAPEPKAKKSSSSTVTSEKITKLYEMGNEPERKMWVDRYLSFMEERGTPVPNLPAVGKKPLDLCRLYMSVKDIGGLAQVNKNKKWRELSTNLNVGTSSSSASSLKKQYIQYLFAFECKVERGEEPPPEIFAMGDNKKQAKIQPPSPANSGSLQGPQTPQSSSSSSVAEMPGDLKPPTPASTPHAPMTSLQGGRNSGVSVQDLFSDIGDPAFQKQNSMAPGAPFQQGMNMPEVMMRMQFEANKDPFGGMRKVPGSSEPFMPGQLPNSGMPDMYSRTPSGSMSGLGMSHRQQYPYGPGYERRHEPYGQQYPGQGPPAAQMPYGAHQPGMFPQQQAYKRPMDGMYGPAAKRHEGDVYNVPYGGQQQDVYGQYGSGYSGPERRPLQSQYPYPYSRDRMQTSGHTPPQPAQQHGISSQMLSGPLQQSSSSGPAEGPQPNVWPSRTDLPYPYPGRQAPGGSSQVPPYPALGRGDDLMAPEPRLNPDGQWPGHVGQRQSSFLPPSSSSLPPMTTRPPPSSYQTSPAMHNHMPRAPSPGAFQRSMEARMSPNKTPFLPAMKKVGPPLPATQVSGPPSQPPPIVRREIAFPPGTVEATQPVLKPRRKITSKDNAHSLCLDVWIVTMQTASNLPNSCSQGNECARKCITYLSSVVLSLNATGSQLPGFLELIVEYFRRCLIEIFGILKEYEVGSVGQKTLLEPPPSKKEDLPSSESEDEGAQAEGDQEEALCEEEEEEEEGPEPPEAPRPEVAEKEASAGPQEGRGAPEGEEEEKPKQASRFDRLPVKVVKREDDFVVDRSDQLGQAQEFHSGLLHWRVGGGDSTEHIQTHFESKMELSAQRGRPGCPQSSRPEPRTRGRAETEEGGAPEEPEKRIMATIDDVLCTRAGALPEDDPFPARAGTFPFRIHHAKSHCNIRLLEDEPRTLDEAPLSTVASWQDSLTRRCVCVSNIVRSLSFVPGNDAEMSRHPGLVLILGKLVLLRHEHPERKRAPQTYEKEEEQDRGLACSKDEWWWDCLSALRENTLVTLANIAGQLDLSVYPESICLPILDGLLHWMVCPSAEAQDPFPLAGPSSGLTPQRLVLETLCKLSIQDGNVDLLLATPPFSRQEKLYATLLRYVGERKVQVYREMAVAVLSNLAQGDTLAARAIAVQKGSVGNLIGFLEDGVTVAQYQQSQHGLHHLAPPPMEPPSVNMMCRAAKALLAMARVEENRSEFVLFEGRLLDISISSVLNSAVAAVICEVLFHIGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK07</accession>
    <name>W5NK07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01034291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK07"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020966"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021002.1">
        <property type="protein sequence ID" value="ENSLOCP00000020966.1"/>
        <property type="gene ID" value="ENSLOCG00000016958.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686760"/>
    <dbReference type="KEGG" id="loc:102686760"/>
    <dbReference type="eggNOG" id="KOG1584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163137"/>
    <dbReference type="HOGENOM" id="CLU_027239_1_0_1"/>
    <dbReference type="InParanoid" id="W5NK07"/>
    <dbReference type="OMA" id="HWVAEVI"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016958">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="36"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="290" mass="33716" checksum="2046340883BD51AD" modified="2014-04-16" version="1">MPEQTELIHSFKGIPFSTTVSTELLQALDTFEAREDDVVLVSYPKSGTHWLTEIMKNLYHCNNDNNATGQVTLTSALEFGDLSKFDELRNLPSKRLIPTHLSHDMIPVQFKTKKCKMIYVIRNPKDTAVSMFHYYQQNPLLPKIEKWSTFLDMFLKGEVVYGSWIDHVQSWEKNQNDENTLFLYYESMKKDLPKYVKEISSFLNISVSEDQINEIVKKSSFSEMKEKAQKEKVDTKHTVCALTSDKKLIFRKGAVGDWKNHFTSKQNAQFEALFKDKINSSELARQVEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL93</accession>
    <name>W5NL93_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NL93"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021439.1">
        <property type="protein sequence ID" value="ENSLOCP00000021402.1"/>
        <property type="gene ID" value="ENSLOCG00000017297.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282547"/>
    <dbReference type="HOGENOM" id="CLU_058290_0_0_1"/>
    <dbReference type="InParanoid" id="W5NL93"/>
    <dbReference type="OMA" id="KNCDESQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017297">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="14-3-3"/>
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        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="1433ZETA"/>
    </dbReference>
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        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="6"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="225" mass="25521" checksum="10FE3304B91FCBF5" modified="2014-04-16" version="1">PLSPGEQVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTAADGSEKKLEMVRAYREKIEKELEAVCGDVLALLDKFLIKNCSEPQFESKVFYLKMKGDYYRYLAEVAAGERRAAVVESSEGAYKEAFEISKEHMQPTHPIRLGLALNFSVFYYEILNAPEQACHLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDEAGETHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLU7</accession>
    <name>W5NLU7_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NLU7"/>
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        <property type="protein sequence ID" value="ENSLOCP00000021606.1"/>
        <property type="gene ID" value="ENSLOCG00000017501.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_045922_2_0_1"/>
    <dbReference type="InParanoid" id="W5NLU7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
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        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC"/>
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        <property type="entry name" value="Znf_CCHC_sf"/>
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        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
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    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="164" mass="19170" checksum="87A1D87CDB0FE00E" modified="2014-04-16" version="1">AAQRDGKLENYKCVGLQSDNERMVILHMFDPFMPDCDVTTFLRRYVEVQEPPKLIIDRRRVWTGKRQYRVELRKDPRSPGGLAHPPATFAIGTGRGYLYYGNQPVFCRKCREYGHREADCKVTECRKCNKRGHETRHCPIKECSLCGATGHLYRDCRRRNADFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNY2</accession>
    <name>W5NNY2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Neuropeptide Y receptor Y4-2</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202047.1">
        <property type="nucleotide sequence ID" value="XM_015346561.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5NNY2">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022341"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022382.1">
        <property type="protein sequence ID" value="ENSLOCP00000022341.1"/>
        <property type="gene ID" value="ENSLOCG00000018240.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690594"/>
    <dbReference type="KEGG" id="loc:102690594"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161927"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_1_1"/>
    <dbReference type="InParanoid" id="W5NNY2"/>
    <dbReference type="OMA" id="RFPMSTV"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018240">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15397">
        <property type="entry name" value="7tmA_NPY4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000062">
        <property type="entry name" value="Neuropeptide Y receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001933">
        <property type="entry name" value="NPY4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF25">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01015">
        <property type="entry name" value="NRPEPTIDEY4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="66"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="384" mass="43748" checksum="634FA39C4F39DE29" modified="2014-04-16" version="1">MGAMNRTRFPGLDEVPPGFSTNKSFFFNNSTNALPPFLEEPCNVSPGVTVFLVASYCSVMAVGLVGNVCLICVIAKQKEKANVTSIFIANLSFSDIMVCAFCLPFTVVYTLMDYWIFGEVLCKMMPFVQCMSVTVSILSLVLIALERHQLIINPAGWKPTVPQAYLAVVIIWMLACFISLPFLAFHILTDEPFKMLSLLTASLEDKAVCKENWPSHQQKLIYTMWLLVFQYCAPLLFILVCYVRIYARLRKRKDMLDRTCEDNRRMTHSKRINVMLVSLVTVFAVCWLPLNTFNMVADWNYEAVMYCHHDLIFSLCHLMAMVSTCVNPIIYGFLNNNFKKEVKSVVLHCKCSPVEEDYEHFPLSTMHTEVSKTSLRLSCRSNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVM2</accession>
    <name>W5LVM2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Functions as a zinc transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Zinc ion transporter mediating zinc entry from the cytosol into the lumen of organelles along the secretory pathway. By contributing to zinc ion homeostasis within the early secretory pathway, regulates the activation and folding of enzymes like alkaline phosphatases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 16">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Golgi apparatus</location>
            <location evidence="16">trans-Golgi network membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVM2"/>
    <dbReference type="FunCoup" id="W5LVM2">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000179"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000179.1">
        <property type="protein sequence ID" value="ENSLOCP00000000179.1"/>
        <property type="gene ID" value="ENSLOCG00000000160.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698254"/>
    <dbReference type="KEGG" id="loc:102698254"/>
    <dbReference type="CTD" id="148867"/>
    <dbReference type="eggNOG" id="KOG1484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159571"/>
    <dbReference type="HOGENOM" id="CLU_013430_0_3_1"/>
    <dbReference type="InParanoid" id="W5LVM2"/>
    <dbReference type="OMA" id="KWRANER"/>
    <dbReference type="OrthoDB" id="78669at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000160">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904257">
        <property type="term" value="P:zinc ion import into Golgi lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045316">
        <property type="entry name" value="Msc2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45755:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 16" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="18">
        <location>
            <begin position="38"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="165"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="195"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="406" mass="44777" checksum="3E6BF77F15E672A9" modified="2014-04-16" version="1">MLPLSIKDDEYKPPKFNLVGKISGWFRSILSDKTSRNLFFFLCLNLSFAFVELLYGIWSNSLGLISDSFHMFFDCTALLAGLAASVISRWRSNDAFSYGYVRAEVLAGFVNGLFLIFTAFFIFSEGVERALEPPDVHHERLLPVSVAGLLVNLVGIFVFQHGGHGHSHGDEGHGHSHSLFNGSLSHGHGHGGFSHGHELKHGHDDHGHGHGHDDHGHDDHGHGHGHDDHGHGHGHHGHGHDHQETHCHEDHSNTPGKGSSKQILQGVFLHIVADTLGSIGVIISAILMQKYDLMIADPICSMLIAILIGVSVVPLLKESIGILMQRTPPSLDHTLPECYQRVQHLQGVYNLQEPHFWTLCTDVYIGTLKLLVAPDADARWILSQTHNIFTQAGVRQLYVQIDVAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVP9</accession>
    <name>W5LVP9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Copine C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVP9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000206"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000206.1">
        <property type="protein sequence ID" value="ENSLOCP00000000206.1"/>
        <property type="gene ID" value="ENSLOCG00000000183.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160442"/>
    <dbReference type="HOGENOM" id="CLU_1791281_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVP9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000183">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF102">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copine C-terminal" evidence="1">
        <location>
            <begin position="74"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="145" mass="15252" checksum="062C515B2AB30B00" modified="2014-04-16" version="1">AVVTEPVLSGQSGLPVSSFCVQAVVTESVLSGQPGLSVSLSCTLSLSPTLLDISVSQVAIDFTASNGDPQSHSSLHYIDQSQPNEYVKALSAVAEVCQDYDSDKSFPAFGFGARIPPNYEVSHDFPLNFNPDNPECEGTAVLNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWM8</accession>
    <name>W5LWM8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000535"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000536.1">
        <property type="protein sequence ID" value="ENSLOCP00000000535.1"/>
        <property type="gene ID" value="ENSLOCG00000000489.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5TB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_5_2_1"/>
    <dbReference type="InParanoid" id="W5LWM8"/>
    <dbReference type="OMA" id="LLSHECQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000489">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001878">
        <property type="entry name" value="Immunoglobulin heavy variable 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867237" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="176" mass="19641" checksum="A0A8900F5CB8777B" modified="2014-04-16" version="1">MQMLIVFFMMALCTGVQADIVLTQSSPQVKKPGDSVTLSCQGSGQDTDENTITDYDVNWIRQAPGKGLEWLGYINNDGDSTNYIPSVKGRFTISRDNSKSTVNLQMSSLKTEDTAVYYCALDTVREVSVLLVQKLFSLCSIEDVEEYKVRSGLSVLFLTNILLTQKVKTWMLPFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWY5</accession>
    <name>W5LWY5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cAMP-responsive element modulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWY5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000642"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000644.1">
        <property type="protein sequence ID" value="ENSLOCP00000000642.1"/>
        <property type="gene ID" value="ENSLOCG00000000580.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156952"/>
    <dbReference type="InParanoid" id="W5LWY5"/>
    <dbReference type="OMA" id="HEKTTER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000580">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141156">
        <property type="term" value="P:cAMP/PKA signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14690">
        <property type="entry name" value="bZIP_CREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000003">
        <property type="entry name" value="cAMP-responsive element modulator isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879:SF4">
        <property type="entry name" value="CAMP-RESPONSIVE ELEMENT MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02173">
        <property type="entry name" value="pKID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50953">
        <property type="entry name" value="KID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KID" evidence="14">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="13">
        <location>
            <begin position="305"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="39005" checksum="323200659BED3AEA" modified="2014-04-16" version="1">LEFCMKHELLPDVLGPMTMETVVDSQQDGSVNDSVSDGEGVQIKNRSTQSPISTIGQATVTSSGVTGVSSSVTVVQLPSGQTVQVHGVIQAPQPSVIQSPQIQTVQVAAIAESDDGETTEGVTDSQKRRELLSRRPSYRKILNELSSDSPAVPKIEEEKSEEEGTPSNVVPQPVPTSIYQTSTGQYIAIAQGGPIHIASPGSDGIQGLQTLTMTNSGTPQPGATIVQYATQSGDGTQQFFVPGSPLVVQENDSDVELDPIPLASITGDMPTYQIRTPTSSLPQGVVMAASPGSLQGPQQLAEEATRKRELRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXR8</accession>
    <name>W5LXR8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LXR8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000925"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000929.1">
        <property type="protein sequence ID" value="ENSLOCP00000000925.1"/>
        <property type="gene ID" value="ENSLOCG00000000831.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2037">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160153"/>
    <dbReference type="HOGENOM" id="CLU_602102_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXR8"/>
    <dbReference type="OMA" id="WHDSINC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000831">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Ring finger protein 112, gene 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_GB1_RHD3_dom"/>
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    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="5">
        <location>
            <begin position="54"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="478" mass="53395" checksum="6AA4695CED0AB07F" modified="2014-04-16" version="1">RAVASTLTRVISERGALPQGSGRPIQLVKVSSDGSLLFQEDVLRQCFLRPDVGETPVCVVSIVGERRKGKSFLLNYLLRKLSNMDEWDRMSDSEPLRGFEWQSGSESMTKGVFIWSQPLFLETQEGKLAVFLVDTEGSVDLSREMELSVKLCALSVFLSSHVIFNVPSDVTTTDLDYLELLHLVVQKGGAAFDLQHFQGFYFLVRDWTQTQECGQTGGSRHLERIIQRLEKSGTNKEALRILKEKRPRCHLMPLPGNKLLWSQQGTMQEMDEQFQHCLKEFINHIGQVLQMTRSTVTGKTLEGKFKEMAHMILKSNNRISSPAEELSQFLSSEREKFIIKKEFTDFLNEQLAPSSSEDGKVRLKLPNKMKKTVKRKEKELLDCVKGQIKSRQDFEEFEKYMAAECKTFLKKHKTKFGVKVACIVAGSVLTATGGVAGGLVVGGVIAAEVGVGVAASAGVGALSAIIKFFKTKTKTKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXS8</accession>
    <name>W5LXS8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXS8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000935"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000939.1">
        <property type="protein sequence ID" value="ENSLOCP00000000935.1"/>
        <property type="gene ID" value="ENSLOCG00000000837.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S38K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154988"/>
    <dbReference type="HOGENOM" id="CLU_000680_8_2_1"/>
    <dbReference type="InParanoid" id="W5LXS8"/>
    <dbReference type="OMA" id="CHITVIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF67">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29587" checksum="2569E3855044DB5B" modified="2014-04-16" version="1">ACNVRTLMDSVSNMRPERRMALTACYSIDIAALSETWLAEEGSISEPKGGYTFFWKGTVENEDRTHRVLFTIKISLLNQPASMSLIKLPFPLSPFCHITVISGYAPTMTCSDEAKESFYSDINGDKPLLMDDFNARVGNDFRIKNGVLEHHGANKMNTNGLLLLSFCGENDLSITNTLFRQADKYKTTWMHSRSKQWRMTDFIIRNSWDICDFKITSALCGEKGLTDHRLVKSVLILHIIPTHHKKPKFIRQSFNTGKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYP7</accession>
    <name>W5LYP7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Ectonucleoside triphosphate diphosphohydrolase 2a, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYP7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001254"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001258.1">
        <property type="protein sequence ID" value="ENSLOCP00000001254.1"/>
        <property type="gene ID" value="ENSLOCG00000001110.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286965"/>
    <dbReference type="HOGENOM" id="CLU_010246_2_3_1"/>
    <dbReference type="InParanoid" id="W5LYP7"/>
    <dbReference type="OMA" id="NEECRYQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001110">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000002">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000068">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF33">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="506" mass="55702" checksum="10B2E8BB3FA3E0D6" modified="2014-04-16" version="1">SARTMARHCGQLATPVLLLLAGAFGIMLLAIPTRDVEEPPDYMYGIVLDAGSSHTALYIYKWPADKENGTGIVTQHSECHVEGGGISSYASQPGGAGKSLETCLNQAKQDVPQGKHAQTPIYLGATAGMRLLNMSSPEDSDRVLMEVKEKVSSYPFKFQRAEILTGQLEGAYGWVTINYLLENFIKYGFVGRWLSPGQGTVGAMDFGGASTQISFVTQEKLESPLDSMTLRLYGQEYRLYTHSFLCYGRDQALRKVLASMMQTQNFVAEVTHPCYPAGHSVDVKLGQVFDSPCTQGQRPSPYRPDSVVRVLGSGNYGECRNVVSQIFSLQDCPFSRCSFNSVFQPPVSGRFLAFSAFFYTSSFLQRATGISVNSPAQMKSAAQAVCAMTFKQMREKVPDQEKRLQDYCTISVFIEVLMLDAYGFDNSTFPLVSFQKQVGDTSIGWALGYMLSLSSLLPEESLSVRKALRPWAWAVLLLLFVLLLLLALVLLLLHLCRSRKSGGAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZW9</accession>
    <name>W5LZW9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Erythrocyte membrane protein band 4.1 like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZW9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001676"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001681.1">
        <property type="protein sequence ID" value="ENSLOCP00000001676.1"/>
        <property type="gene ID" value="ENSLOCG00000001454.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158442"/>
    <dbReference type="HOGENOM" id="CLU_003623_3_1_1"/>
    <dbReference type="InParanoid" id="W5LZW9"/>
    <dbReference type="OMA" id="WEERDIP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001454">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF24">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04382">
        <property type="entry name" value="SAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002304">
        <property type="entry name" value="Membrane_skeletal_4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="54"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="521"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="577"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="721"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="877" mass="98722" checksum="D52EF4710658A86B" modified="2014-04-16" version="1">QDTANADGKKAKQDHDAKHLDDKDHRDADEASEKTTPSKAPKSPQKTSKRPKTVLFKVTLLDSSEYEDEIEKHSRGQVLLDLVCEHLNLLEKDYFGLTFCDTDSQKNWLDPSKEIKKQIRTGPWHFNFAVKFYPPDPSQLTEDITRYYLCLQLRDDILSGRLPCSFVTHALLGSYAVQAELGDYDPEEHGTDYASEFRFAPNQTRELEERVMELHRNYRGMIPAEAEINFLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYRDRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTIGFKLPNHRAAKRLWKVCIEHHTFFRLVSPEPPPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPHFERSASKRYLLSRSMDGDFLCPASALDENHDGVSQRSASERQRRRSEDEQEGVTTPTKIKELKRGWDDCISDIKQHLCFEELMNSSSRTSMQKVITFRCQEDAESPADNKQEVSSQSDQESTPRHKQEFLDKSEDILLKHQASINELKRALREPNSKLVHREKRLSGTSPGGTPEKKAVSRGGGGEEPVISPGLEGFIQKTVMSSPEEPLSTPAIHEEPLDESQREPWERRTGSASEDDQERETLYLKETRSPSARACGARHLYSISEHLSEAPGELLKELLSSPKEICKGHSCFKIAQPSERFGRNCFACFHLHEVELAKRSFPVEHISIESYKPWPPVAKKDPSAVSVAQSLKKTDSKTDMSPNGAPVTSETVSDPAVQSHSLSSGKEAPPSPQATPLDTSSPVKTSTLGKEAGMSPHSVTTENVTSATTTHVTKTVKGGYSETRIEKRIIITGDDDVDQDQALALAIQEAKQQHPDMLVTKAVVVRETQSTAQEKHSKSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZY7</accession>
    <name>W5LZY7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Interleukin 12 receptor subunit beta 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZY7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001694"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001699.1">
        <property type="protein sequence ID" value="ENSLOCP00000001694.1"/>
        <property type="gene ID" value="ENSLOCG00000001487.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079633"/>
    <dbReference type="KEGG" id="loc:107079633"/>
    <dbReference type="CTD" id="3594"/>
    <dbReference type="eggNOG" id="ENOG502S2KP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155603"/>
    <dbReference type="HOGENOM" id="CLU_412148_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZY7"/>
    <dbReference type="OMA" id="ECSWEYE"/>
    <dbReference type="OrthoDB" id="8945484at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001487">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="IL11/IL27_subunit_beta"/>
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        <property type="entry name" value="INTERLEUKIN-12 RECEPTOR SUBUNIT BETA-1-RELATED"/>
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        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
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            <begin position="20"/>
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            <begin position="449"/>
            <end position="541"/>
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            <dbReference type="ARBA" id="ARBA00008921"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M001</accession>
    <name>W5M001_LEPOC</name>
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        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000001708"/>
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        <property type="protein sequence ID" value="ENSLOCP00000001708.1"/>
        <property type="gene ID" value="ENSLOCG00000001497.1"/>
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    </dbReference>
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        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="chain" id="PRO_5004865415" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="12717" checksum="D929243417F2A981" modified="2014-04-16" version="1">MSLSAGVLLVLLFVQYASGDVLLTQSSSEIGKPGQSLRLSCAVSGYSLTDSSYATSWIRQPPGKRLEFLAEIYHDGSANYQDSIKGRFTVSKSGGSVYLQMNSLQSDDTAVYYCARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1D0</accession>
    <name>W5M1D0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SID1 transmembrane family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SID1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1D0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002187"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002192.1">
        <property type="protein sequence ID" value="ENSLOCP00000002187.1"/>
        <property type="gene ID" value="ENSLOCG00000001852.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUXZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010091"/>
    <dbReference type="HOGENOM" id="CLU_357018_0_0_1"/>
    <dbReference type="InParanoid" id="W5M1D0"/>
    <dbReference type="OMA" id="GLHQTMT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001852">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051033">
        <property type="term" value="F:RNA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050658">
        <property type="term" value="P:RNA transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025958">
        <property type="entry name" value="SID1_TM_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12185:SF16">
        <property type="entry name" value="SID1 TRANSMEMBRANE FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12185">
        <property type="entry name" value="SID1 TRANSMEMBRANE FAMILY MEMEBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13965">
        <property type="entry name" value="SID-1_RNA_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867111" evidence="9">
        <location>
            <begin position="36"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="735"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="853" mass="95770" checksum="1DAA6E90EC4B9E9F" modified="2014-04-16" version="1">MATRSCWKSRHKSGTAAGPAALLALCLGLCQTAAGQHAGAAAKTVVQKDGEFDTTYNDTANSEQQTIYAFNHTVSRNKTEGVRVSVIVFSDGAENPISFVVRQKQAVVSFQVPLILRGLYQRKYAYPHVGRTLCQPPARAEMETQFFFVDVSTLSPASVQYQLRISRVESFTLQTNKKSSFTATPSQPQYFKYVFPEGVDSVVVKVNSEMTFPCSVISIQDILCPVYDLDNNVAFIGMYQTMTTTAAITVQRKDFPSNSFYVVVVVKTEDEACGGPLRFYPLQPDELIDAGNRSKALDVLVSPAIKPEVYVMGMLFCLGIFLSFYLLAFLVACLENKSTRLHRKREGLNTPDASPAETASLLGKGLTPASLCQPYDYGSFDPFFPDNGSTVSSEAVTDSMASGDAHYMERPLDHVNRGRQESLSSVEEDDYDTLADIDSDKNIVRTKKYLCVSDLARKDKRVLSKKYQIYFWNIATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLCAHPLGALSAFNNILSNLGYVMLGLLFLLIVLQRDILHKRALMRNELSALECGIPKHFGLFYAMGTALMMEGLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLYQKRHPDINASAYTAYACLAAVIFFSVLGVVFGKGNTVFWIVFSVIHILATLLLSIQLYYMGRWRLDSGVVRRMLYVVYTDCIRQCSGPMYIDRMVLLVMGNIVNWSLAAYGLIVRPNDFASYLLAIAICNLLLYFAFYIIMKLRSGERIKLMALVCIFFTAVVWGFALFFFFQGLSTWQKTPAESREHNRDCILLSFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDCVQRDKIYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M313</accession>
    <name>W5M313_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-azacytidine-induced protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M313"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002771"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002777.1">
        <property type="protein sequence ID" value="ENSLOCP00000002771.1"/>
        <property type="gene ID" value="ENSLOCG00000002349.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV07">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153704"/>
    <dbReference type="HOGENOM" id="CLU_059745_0_0_1"/>
    <dbReference type="InParanoid" id="W5M313"/>
    <dbReference type="OMA" id="PWPSQSC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002349">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024581">
        <property type="entry name" value="TBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051891">
        <property type="entry name" value="TBK1-IKBKE_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432:SF6">
        <property type="entry name" value="5-AZACYTIDINE-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432">
        <property type="entry name" value="PROSAPIP2 PROTEIN/5-AZACYTIDINE INDUCED GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12845">
        <property type="entry name" value="TBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Tbk1/Ikki binding" evidence="10">
        <location>
            <begin position="231"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="382" mass="42694" checksum="25F7BEC33145243C" modified="2014-04-16" version="1">PAISLNTGTMDSLAVEDDICILRHETADSLLQPSKESPVSASSGDESVASHFALVTAYEDIKKRLKEAEKENALLKKRVKQLEEKLFKPHELTSEGSKYVNQAYNAYVGVYMEKTDLQRELDKVKREKTESETLLNEQLQAKELELLQLRTDVETSQVMKTMNSTQDYWDVYKVNSELKIHTLQQELELLHLEYNRLQHTYCRCRTVPHTQEGTDGLKKRSLFTFALVDASLLKTYVDLRREMSNLYAVTKSQAKMVRKLRDSSQSAVSRGPASVPVQCFEDVENNHRLVRVTAPGSPLAGSGPGPTPAQGPTGELWPDPWTSPRPPPVGSPGSYARTSLDDGSWSFPSPPKPSDALFWEAGRSASSSDAPKSPGPDWLRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3C1</accession>
    <name>W5M3C1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Teashirt zinc finger homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220160.1">
        <property type="nucleotide sequence ID" value="XM_015364674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3C1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002879"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002885.1">
        <property type="protein sequence ID" value="ENSLOCP00000002879.1"/>
        <property type="gene ID" value="ENSLOCG00000002451.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693917"/>
    <dbReference type="KEGG" id="loc:102693917"/>
    <dbReference type="eggNOG" id="ENOG502QV71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183051"/>
    <dbReference type="HOGENOM" id="CLU_010469_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3C1"/>
    <dbReference type="OMA" id="TGHYQDN"/>
    <dbReference type="OrthoDB" id="5815793at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002451">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058631">
        <property type="entry name" value="TSHZ1-3_homeodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF3">
        <property type="entry name" value="TEASHIRT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26094">
        <property type="entry name" value="HTH_TSHZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="275"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="924"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="991"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="431"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1030" mass="113243" checksum="C8D8DBD06262CFC4" modified="2014-04-16" version="1">MPRRKQQAPKRAAVYVPEEDINLRETSAGDEGENDSPPEEKCHDKASAEEPEEKETDNKSSYSYQNSPVSAASNQEAELESRLSDASDRLSDFKSISPSGSQKDEDTKSLKLKDEMQNSLEKMRAAYANFLSDSYWSGIGLDLKVGANSGKGSCDSTNGSTKSDFDWHQDALSKTLQQTLSPKPASKPNLFSSVHLYRQNSKTCGTVFTGASRFRCRECSAAYDTLVELTVHMNKSGHYQDDNHNRHGNASTSCSKTRKRNLQDMEGKEDAQKVLKCMFCGHSFDSLQDLSVHMIKTKHYQKVPLKEPIPVIASKIVPPAKKRAYEATRPCSPDSTTGISGYTDSRASGFHNSHNRYGYQNGASYTWQFETCKSQILKCMECGSSHDTLQQLTTHMMVTGHFIKVTSSASKKGKQLALDPLAVEKMQSLAEPPVNETQSDPEGAKASPNSFELGETEIQKEKGPETTGNKTEEKDGKDGKQETGEQKSGEASFKYPYLREEDLEQESEGGGDILKSLANTVASAINKAQTGTPSWSAYPSIHAAYQLSGVMKNSNLPVSQPIQLKQAFNNRLRPIAPKGKFYQAAPGIESFAGHLHNVEIKKEQSSPSDDKEAQIGTADQKAPAESDVQNESSVSTQADKDSVKDSTETMKGNLTPDFSEGNKKSPSPPASNGGGNVSALINDSPEMLGVNPLSALQSVLNNHLGKANKPSHPRPEATLTASTQAMFSKLSRSLEKPALALVPPRASSLNSSFLFESNDQPIDLTKSKSSKTQATSRVQSCTPVPQKHALSDIADMVKVLPKATTPKPPMPSRMPSMKLETDVRRFEDVSAEVYSVHKRKGRQSNWNPQHLLILQAQFASSLFQTSEGKYLLSDLGPQERMHISKFTGLSMTTISHWLANVKYQLRKTGGTKFLKNMDTGHPIFYCNDCASQFRTPSAFINHLESHLGFQIKDMNKFPIEQQTKVEPELPKAIGVKAIEGPSVDEEGDSKFKCKLCSRTFASNHAVKLHLSKTHSKSPENHSQFVEMDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3I6</accession>
    <name>W5M3I6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 4 regulatory subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. Plays a role in the inhibition of TNF-induced NF-kappa-B activation by regulating the dephosphorylation of TRAF2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R1. Interacts with HDAC3.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3I6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002944"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002950.1">
        <property type="protein sequence ID" value="ENSLOCP00000002944.1"/>
        <property type="gene ID" value="ENSLOCG00000002460.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183066"/>
    <dbReference type="HOGENOM" id="CLU_009128_1_1_1"/>
    <dbReference type="InParanoid" id="W5M3I6"/>
    <dbReference type="OMA" id="CYIHNDS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002460">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000156">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000161">
        <property type="entry name" value="serine/threonine-protein phosphatase 4 regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF8">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="173"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="253"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="537"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="584"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="932" mass="105694" checksum="0A0695F4C0DB36CD" modified="2014-04-16" version="1">FADLSLLQGDSQEEIDGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYVASENIFNRQMVARSLLDTLKAVSEDERDCIAVLERVSRLAEDSEPTVRAELMEQVPHIAIFCQENRPSIPFAFSKYLLPIVVRYLADQNNQVRKTSQAALLVLLEQELIERDDVETKVCPVLVDLTAPDSNDDVKTEAMAIICKMAPMVGKDITERLFLPRFCEMCCDCRMFHVRKVCAANFGDICSVVGREATEELLLPRFFQLCSDNVWGVRKACAECFMTVSSATSQEVRRTKLSSLFINLISDPSRWVRQAAFQSLGQFISTFASPSGSGQCFKEEDEGAQEGCRCVPLQSRYPSASPRRVPRPARLESARFPTRLAPPLSSQRAFLALKREEKLFLSLEQRRCCVLSSPQARTRGQGRAWSRGWGCASQAPRRVVGHFRVCERDSSVLKQKPILSIVPIHNIMCKKDTTVFFLFKVNHKLQMWGTEFNSNHNHSIQNRFNRKNMRELEEMIENLEPHTDDPDVKAQVDVLTAALRASALDSHQEDTAQEPLDSRTEHSEEGGSEDPFAVDTVPLICDSAEIESREASLQLSHGEDSELSDSSTSPEEDRKSKQQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRDTYETLASDMQWKVRRTLAFSIHELAVILGDQLTAVRIGVLKHLYDFLKLLHQEKRREYLYQLQEFLVTDNSRNWRFRAELAEQLVLLLELYGGKDVYDYLRPLAFNLCTDRVSSVRWISYKLVSEIIRKLSSDVTLLADFMVELVDKFCHSPKWSGRQAFAFICQLVIEDECVSMEQFSEYLLPPLLQLVSDPVPNVRVLLAKTLRQTLLEKEYFVQSANSHQEALEQTLVALQMDLDKDVKYFASVLPGTTRINDDAMSTASSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4A8</accession>
    <name>W5M4A8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Heart development protein with EGF-like domains 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4A8"/>
    <dbReference type="FunCoup" id="W5M4A8">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003216"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003223.1">
        <property type="protein sequence ID" value="ENSLOCP00000003216.1"/>
        <property type="gene ID" value="ENSLOCG00000002732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691494"/>
    <dbReference type="KEGG" id="loc:102691494"/>
    <dbReference type="CTD" id="57493"/>
    <dbReference type="eggNOG" id="ENOG502QPW9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00710000106813"/>
    <dbReference type="HOGENOM" id="CLU_010549_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4A8"/>
    <dbReference type="OMA" id="ETERPAM"/>
    <dbReference type="OrthoDB" id="9946171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002732">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN HEG HOMOLOG 1"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
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    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
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            <begin position="803"/>
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        <source>
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        <source>
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    <sequence length="935" mass="100300" checksum="A1D7EFD2D24834F5" modified="2014-04-16" version="1">MATCTWINAATLLVMVLSVFKCLCAGSYTARTDPDWITTGESTDFTTNATGFRESTVLPSDVGSHTTASPSRFESASPEKTDSFSGSPEERQTLAGASTATEHSPQTDVHTGTHLEEWKSPSQTDSTYISSRTLLSLSTNSTSPYAEGTQFSDHSQMNTGRSEPWNSTEGDLTTSELTSTGPEHRQSTKGSRHLTTHVSSGENPSTGPPNVTHPSLPTTSDSTPNATPPLNSSWSITEDPETSSSAVMSQSSPSSSKTGFSSSSSSSSVPPSLSSNYSVPFTNSSWADGGGSVTSITHSLTDTDTSTGPPLPRTDNNTDGNQSLPPEDVTHASITTGPPSRTESETTAKDPSTEIGSSESLVTNGSVTELFTSKMEKVEKTTVENPPTTQKSITERTPLTEQDTQVITTSPPVTTRFYAPGTTEETRTIQTEHSTSWATTSQPLSTDSTSQHLSPAGTEGTHTQVSLRTSVRPTNVSTLVLETSTATPGKTPGKTEQTTGFYRSTMVSSTVTSTSATEKLTEKATTITQLTSTTAPATTTLPPVNKCQPNPCANGGKCSPGKSHCICPSSWRGDNCTEDVDECVSSPCPSGSTCVNTKGSFTCECPLGYSLEKGRTCNRAKTFLGTFTVKNVHLANTTDRRFDEHEMQREIIQMLNASLSLLGGYSKSTVTVSSEADGIRCSAVNIFSVTANVTSVDVLRNINEYLKSCTPATTGCCVVHRCSLSYRADSLCAKNNSRCDTERSTCGDSSGIEVCECLPGYFKYSAEDLSCRACNDGYKLENGTCVSCPFGFGGFNCGNFYKLIALVVFPAGGGLLLILLIVLIITCCRKDKNDISKLIFKSGEFQMSPYAEYPKSNRISVEWGRETIEMQENGSTKNLLQMTDIYYSPALRNHELERNGLYPYSGLPGTRHSCIYPGQYNPSFISDDTRRRDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4M5</accession>
    <name>W5M4M5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MLX interacting protein like</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4M5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003333"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003340.1">
        <property type="protein sequence ID" value="ENSLOCP00000003333.1"/>
        <property type="gene ID" value="ENSLOCG00000002824.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3582">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159210"/>
    <dbReference type="HOGENOM" id="CLU_007471_1_0_1"/>
    <dbReference type="InParanoid" id="W5M4M5"/>
    <dbReference type="OMA" id="WTSDRHN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002824">
        <property type="expression patterns" value="Expressed in liver and 7 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19689">
        <property type="entry name" value="bHLHzip_MLXIPL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NES2-NLS_ChREBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
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        <property type="entry name" value="Max-like/E-box_TFs"/>
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    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF41">
        <property type="entry name" value="CARBOHYDRATE-RESPONSIVE ELEMENT-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="690"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="734"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="894" mass="99322" checksum="0C9D9C97316FE850" modified="2014-04-16" version="1">MAKAERLGSAGFSGRTKHDQIPDSESDSDPEEEHGVNAGAGQATGAVSRTQVIHSGHFMVSAPHSDSTPRRGKRGSPAGYDFDTVNRTWCHTYSFGPRSSGSLSIDPTLTRLFECMTLAYSGKLVSPKWKSFKGLRLLWRDKIRLNNGIWRAWYIQYVEKRRNPVCGFVTPLEGSEAEAHRKPEAIVLEGSYWKRRIEVVIKEYHKWRIYHKKRLQKNKNGRLSMLDKSSNILYFRKKHSMFPEPVRMQEESHMFDLDCFLSDISDTLFTMTQKSCPWAGDRHDAYTSNADMIQPGLTPLQPNLDDFMDIPESEYFGSVAGSMFSSGPALGTAEPLLPGNNQLVQANVVPEERLILPPSSNVLSDTGYHRQSLSPKVPYNHSGMTSDPKYKAEVDYNRQSEYHPPELSAATSPYGNKHYTSTTTAVPPSMGSLIQDTMPYGSFSAPCQKYRYTSPITSSVITHTGSMSTVLPEDTPGHVYPAPETFSQVPVSYPPLFGTQTTVPSLAMHHNFSVPQRMAVGCGGTSAGKSKIPVPPASPLQSSCLAKLLSTGTQECGTNMRTSPSLMGAPAVMQQSLSQALEIPLLPALHSHMPQFSPGIQNASSSQSSTVSALLMQNNMPTSTPLLVPKTEKLSPIQIYNEKTALTANSCSWRNSGQISPTNPPEKSPSPQSPHSNPSQTRPEQNKIETRRITHISAEQKRRFNIKLGFDTLHSLVTTLSSQPSIKISKATTLQKTAEYISKMQQERAQLHEEAQRLREEVVALNCAINECQQQLPATGVPITRQRFDHMREMFRDYVRAQTLQNWKFWIFSIIIEPLFETYNGMVSTASVEDLCRTTLSWLDQYCSLPALRPTVLRSLRHLCTSTSILTDPTLVPEQATQAVTQTGPGNSYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6R5</accession>
    <name>W5M6R5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA wybutosine-synthesizing protein 3 homolog</fullName>
            <ecNumber evidence="3">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01018233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6R5"/>
    <dbReference type="FunCoup" id="W5M6R5">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004073"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004081.1">
        <property type="protein sequence ID" value="ENSLOCP00000004073.1"/>
        <property type="gene ID" value="ENSLOCG00000003437.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695863"/>
    <dbReference type="KEGG" id="loc:102695863"/>
    <dbReference type="CTD" id="127253"/>
    <dbReference type="eggNOG" id="KOG1228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153304"/>
    <dbReference type="HOGENOM" id="CLU_047426_1_1_1"/>
    <dbReference type="InParanoid" id="W5M6R5"/>
    <dbReference type="OMA" id="TWLYVSH"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003437">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000001">
        <property type="entry name" value="tRNA wybutosine-synthesizing protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="13">
        <location>
            <begin position="9"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="254" mass="28366" checksum="C8CB40D41185B568" modified="2014-04-16" version="1">MDTEKTFSQWKKQCLSKVDYSKKGSVDEGIAQVVGFLNEYPDYFTTSSCSGRLILIDGVSDSPEVQKRNCSWLFATHQKCRKEDLVSGLEKAGADAVFKFEPFVLHVQCRRLGDAQLLHSVAVNSGFRNSGITVGKRGKIIMAVRSTHGLEVPLSRKGQTLVKEEYLDFLVEVANQKMEENQRRIERFYSSLQSAVRADSSADRSTGLTQDKSVSTRKKKSAQECSREATAQDLEDVILLNGDNGLEDGLHLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M751</accession>
    <name>W5M751_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein lipase</fullName>
            <ecNumber evidence="5">3.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 26">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01011171">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M751"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004209"/>
    <dbReference type="ESTHER" id="lepoc-w5m751">
        <property type="family name" value="Lipoprotein_Lipase"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000004217.1">
        <property type="protein sequence ID" value="ENSLOCP00000004209.1"/>
        <property type="gene ID" value="ENSLOCG00000003542.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692780"/>
    <dbReference type="KEGG" id="loc:102692780"/>
    <dbReference type="CTD" id="30354"/>
    <dbReference type="eggNOG" id="ENOG502QQ7P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157178"/>
    <dbReference type="HOGENOM" id="CLU_027171_1_0_1"/>
    <dbReference type="InParanoid" id="W5M751"/>
    <dbReference type="OMA" id="AIFVKCS"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003542">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034185">
        <property type="term" value="F:apolipoprotein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034372">
        <property type="term" value="P:very-low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000024">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000031">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002330">
        <property type="entry name" value="Lipo_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF3">
        <property type="entry name" value="LIPOPROTEIN LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00822">
        <property type="entry name" value="LIPOLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="18" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0944">Nitration</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="13 27" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0850">VLDL</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867716" description="Lipoprotein lipase" evidence="27">
        <location>
            <begin position="20"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="28">
        <location>
            <begin position="347"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="23">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00018617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022963"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50095"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="499" mass="56978" checksum="DB272A78272F1EA7" modified="2014-04-16" version="1">MGKESLLLFLWIYVAKTWASLAISTEATSLSNTTDWVVDYSNIQTKFSLRTADEPEDDLCYLVPGQPETISQCNFNPNEQTFLVIHGWTVTGMFESWVSKLVYALYKREPASNVIVVDWLHRAHQHYPTSAAYTRLVGQDLAHFLNWIEAELDYPLERSHLLGYSLGAHVAGIAGYLTKKKVNRITGLDPAGPNFEYADEQSSLSPDDAEFVDVLHTNTRGSPDLSIGIKRPVGHVDIYPNGGSFQPGCDLHKAMYMIAAAGFKEMDQLVKCSHERSVHLFIDSLLNEHKQSLAFRCGAKETFDRGLCLSCRKNRCNKVGYEVKKVRSRRSSRMYLKTREIMPYKVFHYQIKLHFFSQKNISFTVQPVQVSLHGTQNETENIALVLPAMSTNKTISFLLTTDVNIGELLMVKMKWEKDSYFSWSDWWGSSSFMIRKLRVKAGETQAKVEFHAQNGDFAELKRGGDFATFVRSKESHVSKRHAREHRRRMYASPWGKKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M921</accession>
    <name>W5M921_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Ubiquitin family domain containing 1</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M921"/>
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        <property type="protein sequence ID" value="ENSLOCP00000004880.1"/>
        <property type="gene ID" value="ENSLOCG00000004087.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_079085_1_0_1"/>
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        <property type="component" value="Linkage group LG13"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd17047">
        <property type="entry name" value="Ubl_UBFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="UBFD1"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="PANTHER" id="PTHR16470">
        <property type="entry name" value="UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1"/>
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            <begin position="64"/>
            <end position="133"/>
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            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
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            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
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            <dbReference type="PROSITE" id="PS50053"/>
        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="32052" checksum="BC04C70C00BB0672" modified="2014-04-16" version="1">MASQDGGESMMQTGASPKKEPVAYGKAGDGDAEAAEQASQSYGENSTAQVSAVSNGSDNDDGKEMVELKIIWNKNKYDLKIPLDGTGASLKERIHSLTGLPPAMQKVMYKGLLPEDKTLREIKVTNGAKIMVVGSTINDVLAVNTPKEVIQQDVKAEENKKEPLCRQKQHRKVLDKGKPEDIMPSIKGAKERLPTVPLSGMYNKSGGKVRLTFKLEQDQLWIGTKERTEKIPMGSIKNVITEPIEGHEDYHMMAFQLGPTEASYYWVYWVPAQYVDAIKDTVLGKWQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5M952</accession>
    <name>W5M952_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="22">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 22">Membrane</location>
            <topology evidence="2 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="22"/>
    <dbReference type="EMBL" id="AHAT01003176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <sequence length="1221" mass="136897" checksum="289E1D8E5BFCC335" modified="2014-04-16" version="1">MYFNRKSKKIHSEGEQLVLTRLSKDTACLSPQSDEEGMGARICSRMSDKRFPGTFDKRPTCSSSGYRKAEDEMSGTTSQADPLDAAARTLVLNQPQLTKFCDNHVSTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIFILTVAGIKEIIEDYKRHKADNTVNKKKTIVLRNGAWQTVIWKQVAVGDFVKVTNGQHFPADMVIVSSSEPQAMCYIETSNLDGETNLKIRQGLPLTAAVQSSEDLVNLAGRLECEGPNGHLYDFTGTLRLDDHNPVPLGPDQVLLRGAQLRNTQWVVGIVVYTGHDSKLMQNSTKAPLKRSNVERVTNMQILVLFCILLVMALVSSVGAAIWNKQHTEAACWYLSRAADISTNFWYNLLTFIILYNNLIPISLLVTLEVVKFTQALFINWDTEMYYAETDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNVMHFKKCTIAGITYGHFPELESYRSMEDFSNLPSSSHSSTEFDDPALIQNIEKDHHTAPHICEFLTMMAVCHTVVPERTGDQIIYQASSPDEGALVKGAKGLGFVFTARTPHSVIIDARGKEQSYELLNVLEFSSNRKRMSVIVRTPTGKLRLYCKGADNVIFERLADDSQYRDLTISHLEQFATEGLRTLCFAYVDLTESSYQEWLKEYNQISTVLKDRAQRLEECYEAIEKNLLLLGATAIEDRLQSGVPETIGTLMRADIKIWVLTGDKQETAINIGYSCRLVTHGMSLIIVNEDSLDATRATLTAHCASLGDTLGKENELALIIDGQTLKYALSFEVRQSFLDLALSCKAVICCRVSPLQKSEIVDMVKKHVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNSSDYSIAQFSYLEKLLLVHGAWSYSRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFALGIFDRPCSQQSMLRSPQLYRITQNADGFNTKVFWGHCINALVHSVILFWFPLKVLEHDSVFNNGRATDYLFVGNIVYTYVVVTVCLKAGLETTAWTRFSHLAVWGSMLLWMVFFAVYSAIWPAIPIAPDMLGQAGMVMKCWSFWLGLLLVPMACLLKDVAWRAGRHTYRKSLLEEVQELEAQAQDPGAAVLRDTNGRSLNERAHLLTRVFKKTPSSLVRSNSVQQTVSHGYAFSQEEHGAVSQSQVVRSYDTTRKRSEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9S7</accession>
    <name>W5M9S7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9S7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005144.1">
        <property type="protein sequence ID" value="ENSLOCP00000005136.1"/>
        <property type="gene ID" value="ENSLOCG00000004300.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1093988_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9S7"/>
    <dbReference type="OMA" id="DPRMRIW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004300">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="254" mass="29010" checksum="B737FD89A1486F14" modified="2014-04-16" version="1">MFSKYFIRLHRTGDQKRFSKGKVPQTYGFSHDGKIARYIQDETMFRFFLLSTISVFLSASTARPHLTWEEVDPGQYQDVVLDLDGKLSVDIRQIEPPEGLAITDEDVHPGMAIWKAVRHVTEQAHLKAEEDRDDLDHPFSAEHLAQALGPRQSPELGIRNAAAAERRQEADELYHAFPRAKDSAQARQDAEFHLPRDFAGLYASQNQEPEDDVDHIHHPPQPGPRHNPAVQTEPVSYEVKHQPEEDRDPIYHGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA79</accession>
    <name>W5MA79_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Fibrillin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005288"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005296.1">
        <property type="protein sequence ID" value="ENSLOCP00000005288.1"/>
        <property type="gene ID" value="ENSLOCG00000004413.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183158"/>
    <dbReference type="HOGENOM" id="CLU_000233_0_0_1"/>
    <dbReference type="InParanoid" id="W5MA79"/>
    <dbReference type="OMA" id="NQCINNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004413">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060958">
        <property type="term" value="P:endocardial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003160">
        <property type="term" value="P:endocardium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048570">
        <property type="term" value="P:notochord morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003250">
        <property type="term" value="P:regulation of cell proliferation involved in heart valve morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049388">
        <property type="entry name" value="FBN_EGF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040872">
        <property type="entry name" value="Fibrillin_U_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF5">
        <property type="entry name" value="FIBRILLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21364">
        <property type="entry name" value="EGF_FBN_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18193">
        <property type="entry name" value="Fibrillin_U_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036312">
        <property type="entry name" value="Fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046921742" evidence="12">
        <location>
            <begin position="22"/>
            <end position="2775"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="183"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="287"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="325"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="489"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="571"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="612"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="658"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="723"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="765"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="807"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="851"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="910"/>
            <end position="951"/>
        </location>
    </feature>
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    <sequence length="2775" mass="300467" checksum="3C7E1B773AFE4740" modified="2025-10-08" version="2">MGKLVLGWAAVCWAALQAAHGQLDGNLQASRFTGRVDERESLRVRRRGQESLRGPNVCGSRFHSYCCPGWKTLPGGNQCIVPICRNSCGEGFCSRPNMCTCSNGQLSPGCGATSVQSCNVRCMNGGSCNDDSCLCQKGYTGTHCGQPVCENGCQNGGRCIGPNRCACVYGFTGPQCERDYRTGPCFTQVNNQMCQGQLSGIVCTKTLCCATIGRAWGHPCEMCPAQPHPCRRGFIPNVRTGACQDVDECQAIPGLCQGGNCINTVGSYECKCPAGHKQSEVNQKCEDIDECSTISGVCGGGECTNTAGSYVCTCPRGYVTSADGSRCVGVHIGSFSGNGRCASELTGQYTKMQCCCDTGRCWAVGQIPEMCPVRGSVRCECAAINRNDPTLIFNASPSSCFHRVALRLTASQTSSNPFGTGNTGLSYIHCTYSIYQIFTLSSKGFSPTQSITEDSRNFTTHFKMTNRIDQIRLLCWIGGSRGLYILPSDVDECVSNPCVNGDCVNTPGSYHCKCHDGYQSTSTKQACIDIDECIVNGVMCRNGRCVNTDGSFQCICNAGFELTPDGKNCIDHDECATTNMCLNGMCINEDGSFKCICKPGFALAPSGRYCTDIDECQTPGICMNGRCINSEGSYRCECPPGLAIDVDGRVCVDTHMRSTCYGAIKMGTCSRPLPGAVTKSECCCTSPEHGFGEPCLPCPARSSAAEFQALCSSGIGITADGRDINECALDPDICPNGMCENLRGSYRCICNIGYESDTSGKNCVDINECLVNRLLCDNGLCRNTPGSYSCSCPTGFVFKADSETCEDVNECESSPCVNGVCRNTAGSFNCECSRGSKLDSTNTVCVDSMKGTCWLNIQDGRCEVNINGATLKSECCSTLGAAWGSPCERCEIDAACPRGFARMKGVTCEDVNECEVFPGVCTNGRCVNTPGSFRCECTEGLTLDGTGRTCVDVRKEQCYLKWDEDECGEPLPGKFRVDMCCCSVGSAWGVDCEECPKPGTPEYKTICPRGPGFASRGDLLTGRPFYKDVNECKVFQGLCSHGTCQNTIGSFKCRCNSGFALNMEERNCTDIDECRISPDLCGHGTCVNTPGSFDCECFEGYESGFMMMKNCMDIDECERDPLLCRGGTCINTEGSYECDCPPGHTLTPEGAACVDVNECELSDNLCRNGRCVNVVGTYQCTCDTGYQVTPDRQGCIGMCGHVQNCGPTVVLSHLGYTYPSSSCVMDYVICRFSSSTADIDECEETPDICDGGQCTNIPGEYRCLCFDGFMASMDMRTCIDVNECDLNPNICLHGDCENTKGSFICHCQLGYFVKKGSTGCTDIDECEIGAHNCDMHAACINVPGSFKCRCRDGWVGDGIKCVDLDECATEEHSCNSNANCVNTPGSYRCTCKEGFNGDGFSCSDVDECADNVNLCENGQCLNAPGGYRCECEMGFTPTEDSKACQDIDECNFQNICVFGTCQNLPGMFRCVCDDGYELDRSGGNCTGEWPDDGFCESLTFSHLKEVLPPAYLRQFCDVHCVSQKEASRSIVGIGFKWKIKVLMSKTGWNSPGFNELQHHREGIDCNCFDKTKQQSMHLDSFTHCVIGYPIPIKNSISDVNECADPVNCINGLCVNTPGSYLCNCPPDFELNPTGVGCVGKCASYWSLSVLCHLVAVAYCLFCLAEYQLLCGNQAPGFIIDIHTGKPIDIDECREIPGICANGVCINQIGSFRCECPMGFSYNNILLICEDIDECNSGENLCQRNANCINIPGSYRCECSPGFKLSPSGACVGQRCLCHNGFKATADQTMCMDIDECDRQPCGNGTCKNTVGSYNCLCFPGFELTHNNDCMDVDECTALVGQVCRNGQCVNSQGSFQCLCQEGYELTLDGKNCVDVNECISLPGTCSPGTCQNLDGSFRCICPSGYSVQNDQCIDINECEVEPNLCLFGECTNTPGSFQCNCQPGFVLSDNGRRCFDTRQSFCYTSFEGMKCSVPKAYNTTKTKCCCSMMPGEGWGDPCELCPREGEAAFLDLCPYGHGSIPGIGDTREDMNECQENPGICVNGICINTDGSFRCECPFGYNLDYTGVNCVDTDECSIGNPCRNGTCTNVVGGFECACQEGFEPGPMMTCEDINECALNPLLCAFRCINTFGSYECTCPAGYTLREDNRMCKDQDECAEGLHDCESRGMTCKNLIGTFMCICPVGMQRRPDGEGCMDTNECRTKPGICKNGQCVNTVGSYRCNCNEGFQASPTGTECIDNRQGFCFTEVLQTMCQQSSTNRNSVTKSECCCNGGRGWGSHCELCPLPGTVQYKRMCPLGPGYTTDGRDVDECRVLPDLCKHGQCINTRGSYRCHCNTGYTTDLTSTTCVDLDECSQSPKPCNFLCKNTEGSYVCSCPRGYILQPDGKTCKDLDECSTKQHNCQFLCINTIGSFTCKCPPGFTQHQSACIDNNECTSQPNLCGTRASCQNTPGSFSCECQKGFSLDGTGLDCEDVDECSSNHRCQHGCQNMLGGYRCGCPQGYVQHYQWNQCVDENECSNSNTCGSASCYNTLGSFKCVCPSGFDFEQSAGGCQDVNECSMGSNPCSFGCSNTDGGYLCGCPGGYFRAGQGHCITGLGFGQGQGQGQGQYLIPEEDDESALSPETCYECKINGNYDKGARHKRSTGDNTLQQESRVSLASVDTQAAIPMNLSLAMLQNKEPILELLPAIEPLEHHVRYVITHGNQNGHFRILERRDGKSVLRLGRKAPAPGSYRLEIASLPIFGPRRLRQLEDEHDANYLLGEIGDALRIKLHIHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAQ4</accession>
    <name>W5MAQ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000005463.1"/>
        <property type="gene ID" value="ENSLOCG00000004564.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
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    <dbReference type="OMA" id="SCCFDAI"/>
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        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004564">
        <property type="expression patterns" value="Expressed in intestine and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="G protein-coupled receptor 55"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF101">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 35-LIKE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="7tm_1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="22"/>
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            <begin position="261"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
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            <begin position="33"/>
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    </feature>
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            <dbReference type="PROSITE" id="PS50262"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="310" mass="35644" checksum="DB676B52F546277A" modified="2014-04-16" version="1">MSRNESCNLTLTSEVKEFQKAAYIPLIVLGLFFNVTALCAFCCRRSTWTVTQVYMTNLIVADSAIILSLPFKVYDSFNNLNLDLCAFVVSVYFGNMYASIFTATAISLQRFTLIQFPFWGRVLKSPRKAIVVCVLIWVIVLTLNVIYYKENLQENKPKCFERTTKPFPVDFILTVEILGFLLPLCTITFCSTQIICTLSRNKNECTQEEKKRSVRIITANLVVFFICFTPIHVGFLLKFIVAKYYPKDCDLLNTMHNFFHISEWIASTNCCLDALGYYFLAKKNHNNPRLAITLQYVAFSTRNSHKLQLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME15</accession>
    <name>W5ME15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein axonemal intermediate chain 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">WD repeat-containing protein 63</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a negative regulator of cell migration, invasion, and metastasis downstream of p53/TP53, through inhibition of Arp2/3 complex-mediated actin polymerization. Via its association with the multisubunit axonemal dynein complex, is potentially involved in the regulation of cilia function. May play a role in osteogenesis of dental tissue-derived mesenchymal stem cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with ACTR2; this interaction reduces binding of the Arp2/3 complex to the VCA domain of nucleation promoting factors. Part of the multisubunit axonemal dynein complex formed at least of two heavy chains and a number of intermediate and light chains. Found in a associated with the catalytic heavy chain DNAH2, the intermediate chain DNAI4, and the light chain DYNLT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME15"/>
    <dbReference type="FunCoup" id="W5ME15">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006624"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006632.1">
        <property type="protein sequence ID" value="ENSLOCP00000006624.1"/>
        <property type="gene ID" value="ENSLOCG00000005471.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156924"/>
    <dbReference type="InParanoid" id="W5ME15"/>
    <dbReference type="OMA" id="WRKRPCD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005471">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036156">
        <property type="term" value="C:inner dynein arm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000415">
        <property type="entry name" value="WD repeat domain 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF5">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="862"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="862"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="920" mass="104694" checksum="2A3E5E318011228D" modified="2014-04-16" version="1">KGNQKSTKTPSTNSKLSGKRPKSSTSIVSNKASGKQKPGSGKHGKKVDSTSNVESAVGQVPPEDIFPLVLTGATQELFQCRADEDVTQENPFKILRKEEILQDMKTRAAVSDFHPVKQLVLNYPGEELLLVFDRDFSYGQSFYLAGTEEAKERILQAPRTTVQEPTDADVVEEESLLEMPVYKTPEPHPWVSLGSEVEIEEESVKDSKKRLRYRASRIRREFGAQVQFTDRNASASKDGYIECPPYQDKSFCIKQLERDTGTQAIPDLWDSSTQTQWKFPKNTWTQYVPREFTEEEKENCMYSENLRNFITSVALRFEIAVQQNEIMDVFFDDWKALGEEESTFGGKADTHLKEYQSFTDLHFSKQKSISYINWHPTINGVIAVAVTERLCFEDRINLSTKLLLSPSLILFWSFSDPINPQLLLECPDDIYCFEFCPSDPNIIVGGCINGQVVLWDISAYREWVQSSRTGGGGKQTPANHGFEEKQENETPIVRYCAVSAIENGHKAPITDVQWLPDSYEISRAGVPLENKDGVCVQIVTCAPDCSVLFWDIRPPRVAAHSMTDRKQKPEERPLDNPHGVPSTFKHLDLTWKPLIKVAFSKIETSGEYSPLKFSRRDGIHDNKMFEDKSLQNLEKNEGGVNYGMLRVPSAKHLKTLDDVSTQIFVGTEDGELIYTDWKMEKDNDSGKMISSKPSHRFVAHDGPVHTVKRSPFFNDIILSVGGWTFAIWKEGVMSGPILQSSCSKKRCTVGHWSLSRPGVFFIGKEDGNIEVWDLLEKTHEPSQTQNITTASITCIKPWIVSSKQHFLAVSDELGTLHILEIPWTLRRPSSNEKSSMSSYFEREVSHLEYFVKRREFRAKEKKDMEAAEQRKKMEPIIPEKSSEQIEEEVQKGYENYLELEKKILKEMGLLPEEEEKLPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEP4</accession>
    <name>W5MEP4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glypican-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cell membrane</location>
            <topology evidence="2 16">Lipid-anchor</topology>
            <topology evidence="2 16">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEP4"/>
    <dbReference type="FunCoup" id="W5MEP4">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006853"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006861.1">
        <property type="protein sequence ID" value="ENSLOCP00000006853.1"/>
        <property type="gene ID" value="ENSLOCG00000005673.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244897"/>
    <dbReference type="HOGENOM" id="CLU_024658_2_0_1"/>
    <dbReference type="InParanoid" id="W5MEP4"/>
    <dbReference type="OMA" id="CNSYCRN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005673">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019803">
        <property type="entry name" value="Glypican_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF8">
        <property type="entry name" value="GLYPICAN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01207">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13 16" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="14 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868041" description="Glypican-1" evidence="18">
        <location>
            <begin position="19"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="552" mass="61736" checksum="298FFF91F05550C8" modified="2014-04-16" version="1">MDLRLSFFFCFLATPIVGENTSGKARSCSDVRQFYSGKGFHLVGVPQTEISGEHLRICPQGYTCCTSEMEESLSTLSRREFEGLVKEAGHSLQLSLTTQYKSFDGYFLDLLNQSERSLQDSFPAVYGELYTQNARLFKDLYAELRRYYRGSNINLEEVLNEFWVRLLERLFKAQNPQYVFGDEYLECAVKQTETLRPFGDVPRELKLKATRAFIAARSFVQGLGVSSEVVRKVSQVPLSPECTRALMKLMYCPHCRGMPSVKPCINYCRNVMKGCLANQADLDTEWRNLIESMLQVADRFNGPSSVEVVIGSIHTRISEAMVNMLDNSAHFTSKVFQACGTPRVGGTQVSGSEDKKKRGKSPVDEKAGLGLEKLVSDVTGKLREMQQYWVQLPNALCTDKVSAAPASEDKCWNGMSRASYLPEVMGDGLASQINNPEVEIDITKPDMTIRQQIMQLKIMTNRLRNAFNGNDVDFQDTSDDVSGSGSGACVEELCPRSTDRNTIPNTDQPIQYAIPAENKKVVKGTASQALPHSTLLLLSLAVVLFRREVNWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF83</accession>
    <name>W5MF83_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF83"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007050.1">
        <property type="protein sequence ID" value="ENSLOCP00000007042.1"/>
        <property type="gene ID" value="ENSLOCG00000005832.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVRJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00420000030019"/>
    <dbReference type="HOGENOM" id="CLU_2020366_0_0_1"/>
    <dbReference type="InParanoid" id="W5MF83"/>
    <dbReference type="OMA" id="RQPQQED"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005832">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="127" mass="13805" checksum="7A4C6DA715FA3922" modified="2014-04-16" version="1">KPLPQGAPGGMIGILGGVVAAGLIIGVAVTVFVVYRRQQKTRTETDNDLTDLPPAHKPAPPPPKKKTSEMKSHLTADDIQVVHLDKPEEEMQKLPLQPPYYDMAPSESTLYSDKSVSSTAHRQELLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF98</accession>
    <name>W5MF98_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GRB2 related adaptor protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF98"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007057"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007065.1">
        <property type="protein sequence ID" value="ENSLOCP00000007057.1"/>
        <property type="gene ID" value="ENSLOCG00000005845.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156254"/>
    <dbReference type="HOGENOM" id="CLU_073617_1_0_1"/>
    <dbReference type="InParanoid" id="W5MF98"/>
    <dbReference type="OMA" id="NKGDMLK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005845">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09941">
        <property type="entry name" value="SH2_Grb2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="63"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="25160" checksum="0473BEBEA1A16115" modified="2014-04-16" version="1">VGRMEAIALYTFKATEGDELSFQKGDVLMITNMEDDPNWYTAELRGRKGYVPKNYINVRPHAWFAGRISRQAAEGRLRHRDCGIFLLRESESAPGEFSLSVSYGDHVQHFKVLKDRGGQYFIWDELFSSLNQLVEFYRTTSIAKERTVFLRDGERVLGRPRQARALFDFTPQHLSQLRFLRGDVIDLLDCADSGRWKGRCQGRVGFFPPEYVQPIYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFE7</accession>
    <name>W5MFE7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFE7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007106"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007114.1">
        <property type="protein sequence ID" value="ENSLOCP00000007106.1"/>
        <property type="gene ID" value="ENSLOCG00000005883.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162428"/>
    <dbReference type="HOGENOM" id="CLU_036904_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFE7"/>
    <dbReference type="OMA" id="EEMEMCL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005883">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072558">
        <property type="term" value="C:NLRP1 inflammasome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050729">
        <property type="term" value="P:positive regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000007">
        <property type="entry name" value="Caspase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF3">
        <property type="entry name" value="CASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="8">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="7">
        <location>
            <begin position="180"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="30747" checksum="C570E42403E88EC6" modified="2014-04-16" version="1">HAPIAQLCQILTLQIYCIGEKNNRTRLAIIINNMKFRFVDSRDGSEVDTKQMKTLLEALDYRVEVVENKTAEEMEMCLRSFSKREEHKMSDSTFVVIMSHGTEEGICGVDYNPQHSRSETILTTKAIGRIFSNDHSLNLVGKPKIIIIQACRGDHKGFVMIPTNKGDDEECLTKYDSDSPLNKIHKESDLVFFCSTTPGTRAYRHRKNGTMFIQRLVETFRNHAWNFHIEDLFKKVQRSFHNFPQQLPCKERTTLLRNFYLFPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG07</accession>
    <name>W5MG07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NSFL1 cofactor p47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">p97 cofactor p47</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">Golgi stack</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG07"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007316"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007324.1">
        <property type="protein sequence ID" value="ENSLOCP00000007316.1"/>
        <property type="gene ID" value="ENSLOCG00000006055.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055567"/>
    <dbReference type="HOGENOM" id="CLU_029402_0_0_1"/>
    <dbReference type="InParanoid" id="W5MG07"/>
    <dbReference type="OMA" id="NKDHTDK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006055">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990730">
        <property type="term" value="C:VCP-NSFL1C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14348">
        <property type="entry name" value="UBA_p47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000020">
        <property type="entry name" value="NSFL1 (p97) cofactor (p47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000093">
        <property type="entry name" value="NSFL1 (P97) cofactor (P47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000001">
        <property type="entry name" value="NSFL1 (P97) cofactor (P47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF24">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="13">
        <location>
            <begin position="180"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="12">
        <location>
            <begin position="292"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="48"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="41142" checksum="E5F483D6457DB245" modified="2014-04-16" version="1">KMADREDSVREFVTVTGVEEERARFFLESAGWDLQLALASFFEDGADDDIVTLPQPEGSSTSRSTAPSEHRVTSFRDLMHEDEEESDEEEGQRFYAGGSERSGQQIVGPPKKKSSNEVVEDLFKGAKEHGAVPVERASKGPGESSRARPFAGGGYRLGTESGEESAYVAGERKQSESAQDVHVVLKLWKTGFSLDNGELRSYSDPENSQFLESIRRGEIPVELRRLARGGQVNLDMEDHREEDFSKPKTMFKAFAGEGKKLGSATPQVIGSIVGTQREADEAQASSEIIINEEEPVTNIQIRLADGGRLVQRFNLTHRISDIRQFVVSARPAMKDVDFVLMTTFPSRELTKESLTLKEADLLNAVIVQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGW0</accession>
    <name>W5MGW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aquaporin-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer. The tetramers can form oligomeric arrays in membranes. The size of the oligomers differs between tissues and is smaller in skeletal muscle than in brain. Interaction between AQP4 oligomeric arrays in close-by cells can contribute to cell-cell adhesion. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGW0"/>
    <dbReference type="FunCoup" id="W5MGW0">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007619"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007628.1">
        <property type="protein sequence ID" value="ENSLOCP00000007619.1"/>
        <property type="gene ID" value="ENSLOCG00000006310.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156037"/>
    <dbReference type="HOGENOM" id="CLU_020019_3_3_1"/>
    <dbReference type="InParanoid" id="W5MGW0"/>
    <dbReference type="OMA" id="AMCHLWA"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006310">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009992">
        <property type="term" value="P:intracellular water homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000009">
        <property type="entry name" value="aquaporin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF34">
        <property type="entry name" value="AQUAPORIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02016">
        <property type="entry name" value="AQUAPORIN4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="35476" checksum="F3CD2EF7D4C97136" modified="2014-04-16" version="1">MKGSYAQVILEKSKARCCSWRCSPLCGREHIMAAFKGIWTQSFWRAVSGEFLATLIFVLLCLGSTINWSAKEKQIPADLVLISLCFGLSIATMVQCFGHISGAHINPAVTAAMVCTKKLSLAKAVFYVIAQCLGAIVGAGILYLVTPSDVRGTLGVTAVSPKISVGHALVVELFITFELVFTVFATCDSKRRDLKGSAALAIGFAVTIGHLFAINYTGASMNPARSFGPAVITGLWEDHWVYWVGPVLGGIIAGALYEYLYCPDPDMKKRLKEAFSKGSYSSGKYKEVEDCRSQGEIEDLAIKPGTVHVIDVDRDEKKDPTSEVLSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH02</accession>
    <name>W5MH02_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dickkopf WNT signaling pathway inhibitor 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630539.1">
        <property type="nucleotide sequence ID" value="XM_006630476.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH02"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007661"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007670.1">
        <property type="protein sequence ID" value="ENSLOCP00000007661.1"/>
        <property type="gene ID" value="ENSLOCG00000006347.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686002"/>
    <dbReference type="KEGG" id="loc:102686002"/>
    <dbReference type="CTD" id="30197"/>
    <dbReference type="eggNOG" id="KOG1218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165723"/>
    <dbReference type="HOGENOM" id="CLU_080459_0_0_1"/>
    <dbReference type="InParanoid" id="W5MH02"/>
    <dbReference type="OMA" id="HIRNHRI"/>
    <dbReference type="OrthoDB" id="4321958at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006347">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23272">
        <property type="entry name" value="Dkk1_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000001">
        <property type="entry name" value="Dickkopf WNT-signaling pathway inhibitor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048500">
        <property type="entry name" value="DIKK1/2/4_C-subdom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048499">
        <property type="entry name" value="DIKK1/2/4_C-subdom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047304">
        <property type="entry name" value="Dkk1_Cys2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF11">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21481">
        <property type="entry name" value="DIKK1-2-4_C-subdom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21479">
        <property type="entry name" value="DIKK1-2-4_C-subdom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868176" evidence="8">
        <location>
            <begin position="23"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="9">
        <location>
            <begin position="74"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 1" evidence="11">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 2" evidence="10">
        <location>
            <begin position="202"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="250" mass="27299" checksum="002E3136F7890258" modified="2014-04-16" version="1">MHLATIPVLLTACFGLYGYSHAVNAGSGLFNSNAIKNLSGGAPNPSYPVSASPDILMYDSGNKNIGADTFQSYSCLDDEECAVDEYCLGSRGVCLSCKKRRKRCVRDAMCCPGNHCSNGLCLPNDPDTTYHAVVDDTIPDRLVDEDQFTVEPHPRRTIVPTGPQSLKGQEGEVCLRSSDCAEGLCCARHFWSKICKPVLKEGQVCTKHRRKGTHGLEIFQRCDCADGLACRTQQGDHSSKASRLHTCQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH70</accession>
    <name>W5MH70_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Leucine-rich repeat-containing G-protein coupled receptor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH70"/>
    <dbReference type="FunCoup" id="W5MH70">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007729"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007738.1">
        <property type="protein sequence ID" value="ENSLOCP00000007729.1"/>
        <property type="gene ID" value="ENSLOCG00000006401.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157925"/>
    <dbReference type="InParanoid" id="W5MH70"/>
    <dbReference type="OMA" id="PPGNCSM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006401">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021888">
        <property type="term" value="P:hypothalamus gonadotrophin-releasing hormone neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15361">
        <property type="entry name" value="7tmA_LGR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF67">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866234" description="Leucine-rich repeat-containing G-protein coupled receptor 4" evidence="21">
        <location>
            <begin position="25"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="666"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="708"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="754"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="789"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="559"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="967" mass="104985" checksum="2D12C34F354EDF17" modified="2014-04-16" version="1">MKLWAARISYAGWFLICGLAAGQGESSPAPCPTPCRCDGDGGADCSGKGLPAVPAGLSAFTYYLDISMNNITELPASVFRNLPYLEELRLAGNDLAFIHPEALSGLQHLKVLMLQNNQLKTVPSKALKGLHALQSLRLDANHITVVPEDSFEGLLQLRHLWLDDNSLMEVPVAPLSNLPSLQALTLALNRISHIPDSAFANLSSLVVLHLHNNKIKEIGEHCFNGLENLETLDLNYNNLLEFPEAIKALPNLKELGFHSNNIAVIPDGAFSQNPLLRAIHLYDNPLSFVGSTAFRNLSELHSLIVRGASMVLRFPDLNGTENLESLTLTGTKIDTIPASLCEDLKLLRTLDLSYNEIEELPSFQGCTKLEEITLQHNRIKGIQADVFRGLASLRLLDLSRNQIRSIHKDAFMSLTSLTNLDLSSNALLTLPTSGLGGLNQLKLAGNMDMKGSLSAKDLQKLRTLSVPYAYQCCAFGGCDAYLNTNPDQGSKSDGSRKKIMYSPGEEVCEGSVAEDEGERIQLAVHCSPPTGAFKPCEHLLGSWMIRLTVWFICLVALLFNLLVLVTTFSSSAAVSPAKLLVGLIAASNLLMGVYTGMLSVLDAASWGHFADFGVWWETGAGCRVTGFLAIFSSEASILFLTLAAVERSVTMRDLAKKGKSRRHRQFRAAAGMLGVSATVAACLPLFHVGDFSSSPLCLPFPTGEAPSLGFTVTLVLLNSTAYLLMAVVYTKLYCGLEKTDLLESSPQASMVKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFLPLPACLNPVLYAFFNPQFKEDWRQIRLCGRWREAKPNAGTAAAVAAGSAAAGLQTGHISPDFYYDCGVYSPLQDNLAVCDCCESLLLAKPVACKHLVKAHSCPALAVVPCQRSDGYWSDCGTPSAQSEYADEGDSFVSDSSDQVQACGRACFYQSRGFPLVHYSYNIPRIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHA1</accession>
    <name>W5MHA1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Reticulon 4 receptor-like 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Nogo receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007760"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007769.1">
        <property type="protein sequence ID" value="ENSLOCP00000007760.1"/>
        <property type="gene ID" value="ENSLOCG00000006422.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157112"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_6_1"/>
    <dbReference type="InParanoid" id="W5MHA1"/>
    <dbReference type="OMA" id="HRKPGKN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006422">
        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="428" mass="48498" checksum="6B070271DB80174A" modified="2014-04-16" version="1">LSPPGCGLEFLLVLCGLELSWSCPRHCVCYTSPTTVSCQAHNFLAVPEGIPPHSERIFLQNNRIHRLLRGHFSPHTVTLWMYSNNITYIEPSTFHGFTQLEELDLGDNRFLRALAADTFHGLSRLHALHLYRCGLSSLPSGIFQGLSNLQYLYLQDNRIEILQDDLFVDLVNLSHLFLHGNQLRSLHQNTFRGLGTLDRLLLHQNRLHWVHHWAFHDLKRLTTLYLFNNSLAELSGECLALLPSLEYLRLNDNPWECGCPARSLWDWLQRFRGSVSSVVCRAPPELSGRDLKQLHKEDFPHCSGSESLHQIKTSTWTTADKVSLKKEXPGRARNCTRHRPRGPKKGLNEVHVLEEMADKEYSAPEFPGDGKYDHTGGAPRRKSKCTPRTPVRPPSGVKQATNRAHSLLGGHLSRALSLLAPLTAIILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI19</accession>
    <name>W5MI19_LEPOC</name>
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        <recommendedName>
            <fullName evidence="5">SEA domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="52190" checksum="152E9CA0534EFCEF" modified="2014-04-16" version="1">MGGEYRALLFLLTGASFIITSLPDDSSSVYGSLELDVTTLNVLSVNTSDIQDEVHRGASSVNITRPYTVTFPSVSAQALESLLNVTVGLEVPGLGCAFVNISELQAAVSTLFHSLAVQTLSETANLPPALLIPMDDKYQATVQYNLSREEVDSPGAILKSLLLALSHKTLSTTKSPMTPTHTTVNNYTSVTTRATPTTTTRVTTSPTTHTTMDDESSASGEEEPLTSTTTRPAPTTTTRVTTSTTTHTTMDDESSASGEEEPLTSTTTRPTPTTTTRVTSSPTTQIIADDDFSGSGEEPFSSATTRPTPTTTTTAITSPTTRIIVDDDFSGSGEDPFTRPTPTTTTRVTTSPTTHITKRDDVTDSGIEEPYIPVTTRSTAKLTTTTRGTSSPTTHTIEDNHSSGSGEEEPFTSTTTRPTETLTTVVSPPPETLTTLHVFLPTTGESLIFQNVTAVHVTMTLRGLKLQTTKGTSTTTIPANTTTTATPTPSTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI72</accession>
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        <submittedName>
            <fullName evidence="4">Zgc:153896</fullName>
        </submittedName>
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    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Lepisosteidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000008091.1">
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        <property type="gene ID" value="ENSLOCG00000006693.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697707"/>
    <dbReference type="KEGG" id="loc:102697707"/>
    <dbReference type="eggNOG" id="KOG2645">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163876"/>
    <dbReference type="HOGENOM" id="CLU_017594_1_2_1"/>
    <dbReference type="InParanoid" id="W5MI72"/>
    <dbReference type="OMA" id="HGMTTIK"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF65">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7-LIKE PRECURSOR"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5004866275" evidence="3">
        <location>
            <begin position="22"/>
            <end position="487"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
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        </location>
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        <location>
            <begin position="467"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="55637" checksum="2AB4F7806B89259B" modified="2014-04-16" version="1">MATISRWFFLSLVLLGQEAKGRPVPGTRRKTFNKLLVISFDGFRWDYDQDVETPNLDYLVHEGVKAKYITPPMITMTSPSHFTTITGRWIEDHGVVHNMMFNPKTLLKLSHKATQNRSEWWDNGVMPLWITAQNQGLKTASFHYPGGGANYSGQSVYRSLVESYNHPDSNETEWRENIETVMDWFSKEDFDFVTLYYGEPDKVGHQKGPETEERRAIIRQIDKTIGFLLEAIERHNLKDRLNVIITSDHGMTTIKKAPVVEEIKLSNYMSFIKLVHFDLLDYGGFGMITPKAGKEEEVYLSLKNVHPHLRVYKKAELPESFHLAKHDRIQPIIVVGDLGYNMNSRVIFYVNKGDHGFSNEEMDMKMIFRAFGPDFKKNYLAEAFDSINIYPLMCKLLGVTPAPNNGSLAFTEGMLVERFDLDPSPVKPEGSPLSASFVALIMLCSLLGVLSLLFISYAGHAVYKRNKGSEPQKEEKPRNEEKKTTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJD6</accession>
    <name>W5MJD6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S15a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the 40S ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJD6"/>
    <dbReference type="FunCoup" id="W5MJD6">
        <property type="interactions" value="1282"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008495"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008505.1">
        <property type="protein sequence ID" value="ENSLOCP00000008495.1"/>
        <property type="gene ID" value="ENSLOCG00000007017.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183198"/>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_1"/>
    <dbReference type="InParanoid" id="W5MJD6"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007017">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="154" mass="17174" checksum="B6D9560D619C3F7A" modified="2014-04-16" version="1">MEEAGGGDGARALTSWSVTLSAGIMVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJP8</accession>
    <name>W5MJP8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein S100-A16-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MJP8">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008607"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008617.1">
        <property type="protein sequence ID" value="ENSLOCP00000008607.1"/>
        <property type="gene ID" value="ENSLOCG00000007099.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SGQU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288572"/>
    <dbReference type="HOGENOM" id="CLU_138624_3_0_1"/>
    <dbReference type="InParanoid" id="W5MJP8"/>
    <dbReference type="OMA" id="YIANSMS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007099">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF115">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN U-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="92" mass="9970" checksum="E2A6117DE45CFDC2" modified="2014-04-16" version="1">MEAAIQTMVKTFQTSSKGKDSLSGKEFQKLVQTQLSNILSDTESAKAVKEMKEGLDTNKDGKVSFQEFMSLVGYIGGMLSQQRSSETXTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK80</accession>
    <name>W5MK80_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polyhomeotic homolog 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK80"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008789"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008800.1">
        <property type="protein sequence ID" value="ENSLOCP00000008789.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2390104at2759"/>
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        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007258">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09577">
        <property type="entry name" value="SAM_Ph1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polyhomeotic-like protein 2 isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
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        <property type="entry name" value="SAM"/>
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    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
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    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF88">
        <property type="entry name" value="POLYHOMEOTIC-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21319">
        <property type="entry name" value="zf-FCS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51024">
        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FCS-type" evidence="11">
        <location>
            <begin position="778"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="927"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="216"/>
            <end position="277"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <end position="410"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <end position="457"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="472"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="661"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="741"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="830"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="237"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <end position="457"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="9">
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            <begin position="477"/>
            <end position="492"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="9">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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            <end position="911"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="991" mass="105413" checksum="A93F2632C04EF2AB" modified="2014-04-16" version="1">MENETSTTTSAPATTTASSPSSRTQAPPPPLYSAPTDRQAVQVIQQAIHRPQSMAAQYLQQMYAAQQQHLMLQTAALQQQHLSSTQLQSLATVQQATLTGGRQSSSPNGSVSQQSNVSQTSINLPSSPVTAQIISRTQSSSSTAAGGAISQQAMLLGNSSPTLSTSQAQMYLRAQMLILTPTATVAAVQSELPVVSSASSPSASTQVQSLTLRAPPSALPSTQTVPLKATTQGQPQIPTHPKMSLCPLKSTQQPEAQGDGGKKGDSPGAEPRNTPVSRLPSAHQLIAPAPFPPVQPHALMKHQLHCPAGHKGAHHQLIIQQQPVAPHRQVQPIALQVTTQETPPPSQHCLSVQPQVAPPTNLVSVQSQHCTSISSAAAPPAPSPEQSQPAAQPTVVVSPPPTHSPTSHSPTIIIQPQALVGTQAQALVSAPMQLGPAQPPPLSSAQVPSSLSSHLSPLSSSLASIDSLPQSALVPPATVPTPQPPQHSPPPQAAAGPLSSPPPPPSGPPELPHPSPTSLQPLPLPSVQALAVQSEVLPAAPMLLSEEELPVAEALVQMPFQTLPPPQTVAVDLQVQPAAPVETPVVQQVYEMEGMCAEEMREDGLSCPLRDRTPTPPALSPPAIPERHCEDMSMHSENCSAVTNLPCVTSTGNASVIRSSADPTYVNSSPPPLLPAVVRSTSKPPQASLPGVPDSKPPQAIVKPHILTHLIEGFVIQEGLEPFPVSRSSLMVQQQVKQPEAQVLKSNGGQPEALLEPEQPENSSDTDMDDMTAEDGVEEAATEVLKCEFCGKRGYAHKFLRSKRFCSMSCAKRFNVSCTKRVSMMKADKVGRWARKPDGRRGRRPSGVEGGSREHFLRQLAVPYNNTEEVQRGASQSPREEEPPVPMTTRLRRQSERERERERDVRMREPSDAAESLPAAQADPTQWTVNDVWAFIYTLPGCQDIAEEFRSQEIDGQALLLLTEDHLMSAMNIKLGPALKICARINSLKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKC4</accession>
    <name>W5MKC4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translocon-associated protein subunit beta</fullName>
            <shortName evidence="11">TRAP-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Signal sequence receptor subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TRAP-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKC4"/>
    <dbReference type="FunCoup" id="W5MKC4">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008833"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008844.1">
        <property type="protein sequence ID" value="ENSLOCP00000008833.1"/>
        <property type="gene ID" value="ENSLOCG00000007294.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005125"/>
    <dbReference type="HOGENOM" id="CLU_102025_1_0_1"/>
    <dbReference type="InParanoid" id="W5MKC4"/>
    <dbReference type="OMA" id="YGFFNYT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007294">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008856">
        <property type="entry name" value="TRAP_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12861:SF3">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12861">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, BETA SUBUNIT PRECURSOR TRAP-BETA SIGNAL SEQUENCE RECEPTOR BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05753">
        <property type="entry name" value="TRAP_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016400">
        <property type="entry name" value="TRAP_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866242" description="Translocon-associated protein subunit beta" evidence="13">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="196" mass="21403" checksum="F79B31F18F3A048C" modified="2014-04-16" version="1">YAIFSFLQMRVLWLCAVLAVVVLVRGEEGARLLASKSLLNRYAVEGRDLTLQYNIYNVGTSAALEVELSDDSFPPEDFGIVSGMLNVKWDRIAPYPCASNVSHTVVLRPLKAGYFNFTSATVSYLAQEGGQVVVGYTSAPGQGGILAQREFDRRFSPHYFVSQHSFAVFTLISLGSTLLILLSLSITVQSPNSPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKI2</accession>
    <name>W5MKI2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MPV17 mitochondrial inner membrane protein like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013631">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKI2"/>
    <dbReference type="FunCoup" id="W5MKI2">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008891"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008902.1">
        <property type="protein sequence ID" value="ENSLOCP00000008891.1"/>
        <property type="gene ID" value="ENSLOCG00000007339.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111088"/>
    <dbReference type="HOGENOM" id="CLU_049109_4_2_1"/>
    <dbReference type="InParanoid" id="W5MKI2"/>
    <dbReference type="OMA" id="AGWWRVL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007339">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF39">
        <property type="entry name" value="MPV17-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="22416" checksum="020EF2556DA64074" modified="2014-04-16" version="1">QSGDWISIFKQAKRFPWITNVTLYGCLFAGGDLVHQCLTHRDGLDWRRTRNVAVVAFSFHGNFNYLWMRALERRFPGRSARMVLQKLLLDQTVASPVATSVFYTGVSLLDGKDDVLQDWREKFLNTYKTGLMYWPFVQLLNFLLVPLYLRTTFTGCCAFLWATFLCFSRHSGDGTAAAAWAWLTAPGAGQQKEQPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKK1</accession>
    <name>W5MKK1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the Deltex family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01032425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKK1"/>
    <dbReference type="FunCoup" id="W5MKK1">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008910"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008921.1">
        <property type="protein sequence ID" value="ENSLOCP00000008910.1"/>
        <property type="gene ID" value="ENSLOCG00000007354.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUYA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161404"/>
    <dbReference type="HOGENOM" id="CLU_030422_1_0_1"/>
    <dbReference type="InParanoid" id="W5MKK1"/>
    <dbReference type="OMA" id="EVCAMLH"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007354">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16711">
        <property type="entry name" value="RING-HC_DTX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039399">
        <property type="entry name" value="Deltex_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="191"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="374" mass="39927" checksum="6BC2DC8487125A56" modified="2014-04-16" version="1">VSLAAMSVRAGRGGDEVLVSQAVWDYLSAAGQPWLAEFQSGHGLSAGVVRRAERGGCCTVKLRSPGGEGPGSGPAPVSPAARRAFMDLCRSARKEMNRKEGAPKRKRSLMPCLGGGEADGEGSLLPPQPRRSQRQQLRCRKAADLGGGLCAPAPPPRDLLPRREAELGGGGAGGGAGGGALPTEVEDGGVCSICMGEMVERTTLDRCGHAFCRTCLDRAFQVKRACPVCRLVYGQLIGNQPANGTMTVERDPDLELPGHEGYGCIFIIYSFPPGTQAQEHLNPGVRYPGTDRVAYLPDSPEGNRVLGLLRRAFEQRLIFTIGTSMTTGMSNVITWNDIHHKTSVWGGPRCFGYPDPTYLVRVTEELREKGITAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKL1</accession>
    <name>W5MKL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="19">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01005195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640336.1">
        <property type="nucleotide sequence ID" value="XM_006640273.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKL1"/>
    <dbReference type="FunCoup" id="W5MKL1">
        <property type="interactions" value="1885"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008920"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008931.1">
        <property type="protein sequence ID" value="ENSLOCP00000008920.1"/>
        <property type="gene ID" value="ENSLOCG00000007358.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699172"/>
    <dbReference type="KEGG" id="loc:102699172"/>
    <dbReference type="CTD" id="5501"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153472"/>
    <dbReference type="InParanoid" id="W5MKL1"/>
    <dbReference type="OMA" id="EEHEIRY"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007358">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="21">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="323" mass="37015" checksum="DD6F2509DE608DC0" modified="2014-04-16" version="1">MADTDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKKPNASRPVTPPRNMITKQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKM8</accession>
    <name>W5MKM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Corepressor interacting with RBPJ, CIR1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008937"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008948.1">
        <property type="protein sequence ID" value="ENSLOCP00000008937.1"/>
        <property type="gene ID" value="ENSLOCG00000007371.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3794">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111135"/>
    <dbReference type="HOGENOM" id="CLU_035642_2_0_1"/>
    <dbReference type="InParanoid" id="W5MKM8"/>
    <dbReference type="OMA" id="CSMYSIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007371">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040014">
        <property type="entry name" value="CIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151">
        <property type="entry name" value="CBF1 INTERACTING COREPRESSOR CIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151:SF2">
        <property type="entry name" value="COREPRESSOR INTERACTING WITH RBPJ 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="52665" checksum="F671AC46DE0C0B22" modified="2014-04-16" version="1">MGKSFANFMCKKDFHPASKSNIKKVWMAEQKITFEKTKQEELMQQYLKEQETYNNRLLMGDDRVKNGLNFMYEAPPGASKGICADQSTKKQEGESDYKFEWQKVAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTDRECPLFGLSGINASSMANDGAAGPSMHPSELMAEMRNSGFALKQSVLGRNVTLCDPSQEYVASEEEEDPEVEFLKSLTTKQKQKLLRKLDHLEKKEKKKKRKGKQKKKKSKSKHKKLQKRSSSSSSDSDSDSDPESHRKRQAEGQKKKKNKKSRRDSSADSSADSESEDRSRSKKRAAARTGRQKRSRSPSTDRSRSGSRKVQGEKRRDDRHVPKQRSGSHGRQGDREDKDKGGKSSSTRESHKSNSTEGVAGNGPQHRCRSRERHADRGERVRSRSKEKHRERKGRDRSDSRGRHRSRSGSPGRERPRKNCERRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLG9</accession>
    <name>W5MLG9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="EMBL" id="AHAT01009960">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG25"/>
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        <location>
            <begin position="85"/>
            <end position="104"/>
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    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009228.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="165" mass="18728" checksum="5B81FA0FED39E4EE" modified="2014-04-16" version="1">GRGGRGAVVRGRRGGEEQEECGCQLLCAAQLEEARGALSLSEQGRDALRREQLEAERRLRESRDEGEGLRREGAELRRSLADLAKERDTLSTSNTQLRDSLRAAESDRISVKRQCEEKEQKLAVMEESRAASEREAGELRAGLREVERSRLEARRELQELRRQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLX8</accession>
    <name>W5MLX8_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2 12">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the NDC80 complex.</text>
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        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Chromosome</location>
            <location evidence="12">Centromere</location>
            <location evidence="12">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SPC24 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045419"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368011"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009387.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="204" mass="23408" checksum="5613420A98242A86" modified="2014-04-16" version="1">CLGMSAMHEGIQALEEVGEVLLDCITNSNAEKTVKTAMEKQTLILSRLLETRKSAAQLVSDLLHTEEKVAHDLLDIQSQRNGVLEELDKLEKELQKSKSRKDMLQAEIEFLQKEIDSLREAEREVQVLQAEVDEDTTEIIPSTKYIVQLYHKVTKIKWDFNTEPDILRGVHFGKDIVTPINLDTREHSEGFISDYLWSLVSTEW</sequence>
</entry>
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    <accession>W5MM15</accession>
    <name>W5MM15_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Spotted gar</name>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000007762.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34756:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="150"/>
        </location>
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        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="188" mass="19837" checksum="71199A1C1F17CEC5" modified="2014-04-16" version="1">MGTSESKVKVAVATPKLEPPSRPVRNKHLQSVIDPRSPSAGIDRTPIQVGAVSSLTPVRGGLSEGAVLEDDPRSPTLGISRTPMKDIMAATVTSFVRRLNDLFLDEEGRAEEGAPETVPRDPYPGVQDEPFVAPPALSETPQLDSPGAVPQNLQAGAQVCREPSRFGCGSPAKDVPRFQTPLLPGSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN62</accession>
    <name>W5MN62_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NK1 transcription factor related 2-like,a</fullName>
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    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NK-1 homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN62"/>
    <dbReference type="FunCoup" id="W5MN62">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000009832.1">
        <property type="protein sequence ID" value="ENSLOCP00000009821.1"/>
        <property type="gene ID" value="ENSLOCG00000008089.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162535"/>
    <dbReference type="HOGENOM" id="CLU_063378_0_0_1"/>
    <dbReference type="InParanoid" id="W5MN62"/>
    <dbReference type="OMA" id="TGNMIFH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008089">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000315">
        <property type="entry name" value="NK1 homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF17">
        <property type="entry name" value="NK1 TRANSCRIPTION FACTOR-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="278" mass="31028" checksum="43644991B7D083F0" modified="2014-04-16" version="1">MLEYQDRGVTMTPCHRISFSIVDILDPNKFTSKKPISFSPGEKFSHAAERESLGRPAEFISNEDPFSRDKDSGGEDRNGQAGCDKADDSERTSLTRQENVTSLERHSEDRDINSYDVDSLANDDVDQTKQKRRRSDHGCAKPRRARTAFTYEQLVALENKFRATRYLSVCERLNLALSLSLTETQVKIWFQNRRTKWKKQNPGADSTIQPGTNSLSRVTSSPGPSGSGTINFQTFHTFSPGNVVFHAAGAVPLSSTGGLLNSFLHTGYLQPTFYSPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP18</accession>
    <name>W5MP18_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zgc:92606</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642471.1">
        <property type="nucleotide sequence ID" value="XM_006642408.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP18"/>
    <dbReference type="FunCoup" id="W5MP18">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010127"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010139.1">
        <property type="protein sequence ID" value="ENSLOCP00000010127.1"/>
        <property type="gene ID" value="ENSLOCG00000008340.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692047"/>
    <dbReference type="KEGG" id="loc:102692047"/>
    <dbReference type="eggNOG" id="KOG1654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166772"/>
    <dbReference type="HOGENOM" id="CLU_119276_0_0_1"/>
    <dbReference type="InParanoid" id="W5MP18"/>
    <dbReference type="OMA" id="VYEEHHD"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008340">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000172">
        <property type="entry name" value="Gamma-aminobutyric acid receptor-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="5">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="13472" checksum="7FE4C89A0D1A4193" modified="2014-04-16" version="1">MGSRYQRSVPLPVRRAEGQRIREKHPDKIPIIVERVARARVPDLQKKKYLVPSDLTVGQFCFLIRQRISLRPEEALFFFVDNALPPSGATLAQIYQDHHAEDLFLYVAYSDESVYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQD3</accession>
    <name>W5MQD3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidyl-[protein] + S-adenosyl-L-methionine = N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19369"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:11600"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16367"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.85"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01003930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQD3"/>
    <dbReference type="FunCoup" id="W5MQD3">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010592"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010607.1">
        <property type="protein sequence ID" value="ENSLOCP00000010592.1"/>
        <property type="gene ID" value="ENSLOCG00000008698.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153577"/>
    <dbReference type="InParanoid" id="W5MQD3"/>
    <dbReference type="OMA" id="QHIDGIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008698">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070472">
        <property type="term" value="P:regulation of uterine smooth muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19176">
        <property type="entry name" value="SET_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1420.10:FF:000001">
        <property type="entry name" value="histone-lysine N-methyltransferase setd3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025785">
        <property type="entry name" value="SETD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044428">
        <property type="entry name" value="SETD3_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51565">
        <property type="entry name" value="SAM_MT85_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="96"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="533"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="568"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="594" mass="67214" checksum="0B031FD57043E7DB" modified="2014-04-16" version="1">MGKKSRVKTQKSGTGAASVISPKEMMNLVSDLLQKCSGPAPAPGKEWEEYIQIRSLVEKIRKKQKGPGLSVVFDGTRDDFFPELMSWAAENGASCEGFEISNFAEEGYGLKATRDIKAEEMFLWIPRKMLMTVESAKNSVLGPLYSQDRILQAMGNVTLAFHLLCERANPASFWLPYIRTLPSEYDIPLYFEEDEVHYLQSTQAIHDVFSQYKNTARQYAYFYKIIQTHPNASKLPLKDSFTYDDYRWAVSSVMTRQNQIPTEDGSHVTLALIPLWDMCNHTNGLITTGYNLEDDRCECVALQDYKQDEQIYIFYGTRSNAELVIHNGFFHDNNSHDRVKIKLGVSKSERLYAMKAEVLARAGIPSSSIFALHCSEPPISAQLLAFLRVFCMTEDELKEYLVGDHAIDKIFTLGNAEFPVSWENEIKLWTFLETRTTLLLKTYKTSTEDDRSLLEKPDLSFHSKVAIKLRLAEKEILEKAMASGKSKREHFQKQLEEGAPLPKYEESNISLLENSVSDSKLPIILRKLEEADEAGEEELKMNEALSDENAGGDVLVNGDSKQLSGTETENRETPYGETGDAKQNGKDVSEAERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR32</accession>
    <name>W5MR32_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted frizzled-related protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR32"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010841"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010857.1">
        <property type="protein sequence ID" value="ENSLOCP00000010841.1"/>
        <property type="gene ID" value="ENSLOCG00000008904.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160608"/>
    <dbReference type="HOGENOM" id="CLU_054647_0_0_1"/>
    <dbReference type="InParanoid" id="W5MR32"/>
    <dbReference type="OMA" id="CINGYHE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008904">
        <property type="expression patterns" value="Expressed in heart and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07444">
        <property type="entry name" value="CRD_SFRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03580">
        <property type="entry name" value="NTR_Sfrp1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000003">
        <property type="entry name" value="Secreted frizzled-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000001">
        <property type="entry name" value="secreted frizzled-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041761">
        <property type="entry name" value="SFRP5_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF46">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866651" description="Secreted frizzled-related protein 1" evidence="12">
        <location>
            <begin position="35"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="13">
        <location>
            <begin position="54"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="14">
        <location>
            <begin position="187"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="36271" checksum="1012D131D731F8B6" modified="2014-04-16" version="1">VSFRNMAIPRNQASPTKAMALALGLFFLTSVASAEEYDYYSWQSDNFHNGRFYTKQPQCVDIPADLRLCHNVGYKKMRLPNLLDHESMPEVKQQAGSWVPLLAKRCHADTQVFLCSLFAPVCLDRPIYPCRSLCEAVRDSCAPVMETYGFPWPEMLQCEKFPIDNDLCIPMQFSGNHATQTPASKVCPPCDNELKADTIMEHFCASDFALKMKIKEVKKEKGDRKLIAAQKKKKVLKMGVLRKKDLKKLVLYIKNGANCPCTQLDNLGTNFLIMGRKVDQQLLLMSIHKWEKKSKELKFAVKYMKSHQCPTYHTVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRI5</accession>
    <name>W5MRI5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Solute carrier family 25 member 28</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630495.1">
        <property type="nucleotide sequence ID" value="XM_006630432.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRI5"/>
    <dbReference type="FunCoup" id="W5MRI5">
        <property type="interactions" value="1792"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010994"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011010.1">
        <property type="protein sequence ID" value="ENSLOCP00000010994.1"/>
        <property type="gene ID" value="ENSLOCG00000009024.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694998"/>
    <dbReference type="KEGG" id="loc:102694998"/>
    <dbReference type="CTD" id="81894"/>
    <dbReference type="eggNOG" id="KOG0760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157049"/>
    <dbReference type="HOGENOM" id="CLU_015166_3_1_1"/>
    <dbReference type="InParanoid" id="W5MRI5"/>
    <dbReference type="OMA" id="WRPMRGM"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009024">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000027">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000031">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF20">
        <property type="entry name" value="MITOFERRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="87"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="185"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="276"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="42272" checksum="B7514CE5EDF9D47F" modified="2014-04-16" version="1">MEADGFVRGRRMSADTPGGEGAVAGASAGAEIRWLGGRLWDVSEGIVGSLSPRIGAGELELQAAQLKAPPEAAGIPEPEYEGLPQGASTSTHMLAGAVAGIMEHCLMYPIDCVKTRMQSLQPEPAARYRNVMDALWRIVRTEGIWRPMRGLNITATGAGPAHALYFACYEKLKKTLSDVIQPGANSHLANGAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYRSVLDCMRAVWQKEGAAAFYRSYTTQLTMNIPFQALHFMAYEYLQETLNPHRRYNPTSHMVSGALAGALAAAATTPLDVCKTLLNTQESLALHSLNVSGHISGLAHAFRTVYRLGGIPAYFKGVQARVIYQMPSTAISWSVYEFFKYFITKHQHEKRKSHRDGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRI9</accession>
    <name>W5MRI9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ras-related protein Rab-20</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Cytoplasmic vesicle membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
            <orientation evidence="18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01003441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRI9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010998"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011014.1">
        <property type="protein sequence ID" value="ENSLOCP00000010998.1"/>
        <property type="gene ID" value="ENSLOCG00000009026.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161024"/>
    <dbReference type="HOGENOM" id="CLU_041217_12_0_1"/>
    <dbReference type="InParanoid" id="W5MRI9"/>
    <dbReference type="OMA" id="DQTECEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009026">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04126">
        <property type="entry name" value="Rab20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001257">
        <property type="entry name" value="RAB20, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041836">
        <property type="entry name" value="Rab20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24073">
        <property type="entry name" value="DRAB5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="239" mass="26550" checksum="894645430B49261F" modified="2014-04-16" version="1">AKMKKPDVKLVILGDMNVGKTSLLHRYIERKFKDTISTVGGAFFLKQWGPYNISIWDTAGREQFHGLGSMYCRGAAAVILTYDVTNWQSLVELEQRFLSLTDTANSDCIFAIVGNKADLTNLKAAAAETGEERESNGNLAGAQFPASPKMHKQVQPEDAVALYHRILRYKMLEEKCSPPAEAMCFETSAKTGYNVDLLFERVFDMVVPGILKKKTEAMSNTVDLEDCSDMQRTKLGCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUH9</accession>
    <name>W5MUH9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUH9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012038"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012059.1">
        <property type="protein sequence ID" value="ENSLOCP00000012038.1"/>
        <property type="gene ID" value="ENSLOCG00000009848.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S65W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162700"/>
    <dbReference type="HOGENOM" id="CLU_027317_1_0_1"/>
    <dbReference type="InParanoid" id="W5MUH9"/>
    <dbReference type="OMA" id="EWRTTIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF112">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="84"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="184"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="280"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="379"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="478"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="544" mass="60350" checksum="33DCB77756B5E574" modified="2014-04-16" version="1">VFVGETVTLNCEVRGSVDWKYYWALEKQGRMVTLRSSSGTYGSQYILRAVSMFDSGQYWCQAERGTDQSDSGFLTLTVIEGQTPAVVTIDPPDSVVFSGERVTLSCVVKAASGGWRYSWARNTKGRLESLFRSSAEDQKNYTLSPATVSDSGQYWCQAERGNKPIIQTHPGFLILSVSGTWPRPILTQNPSSGEIYTGDRVTLSCGVGGDSVDWKYLWYKDIQEAALLNTDSSRTDGSSYTISSAALSHSGEYWCRARRGSVVYYTDHSDSLQIEIIARPQAALTLESGWTEIFTTESVTLRCEIEMGSAGWSYKWYRNGQELPVDSSRRNGDTYTILSAAQLHNGEYTCRGESDHNKLFYSNISNPVKLKVSGKWPKPALTVFPTRGQIYTGDRVTLSCGVGGGSVGWKYLWYKDTQGEVLLNTDSSKTDGSSYTISSAALSHSGEYWCRARRGSVFYYTDRSDSLKLEIIGIWPKPVLTVFPTRGQIYTGDRVTLNCGVGGDFVGWKYLCDTDSSRTNGSSYAIIYAALSHSGEYWCRARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUW0</accession>
    <name>W5MUW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex I-B18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase B18 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFB7 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631829.1">
        <property type="nucleotide sequence ID" value="XM_006631766.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUW0"/>
    <dbReference type="FunCoup" id="W5MUW0">
        <property type="interactions" value="1664"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012169"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012190.1">
        <property type="protein sequence ID" value="ENSLOCP00000012169.1"/>
        <property type="gene ID" value="ENSLOCG00000009951.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690541"/>
    <dbReference type="KEGG" id="loc:102690541"/>
    <dbReference type="CTD" id="4713"/>
    <dbReference type="eggNOG" id="KOG3468">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018759"/>
    <dbReference type="HOGENOM" id="CLU_154847_1_0_1"/>
    <dbReference type="InParanoid" id="W5MUW0"/>
    <dbReference type="OMA" id="FVYQCAH"/>
    <dbReference type="OrthoDB" id="268414at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009951">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008698">
        <property type="entry name" value="NDUB7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20900:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20900">
        <property type="entry name" value="NADH:UBIQUINONE OXIDOREDUCTASE B18-LIKE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05676">
        <property type="entry name" value="NDUF_B7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="121" mass="14626" checksum="99DA92C76269B30A" modified="2014-04-16" version="1">MGAHLVRRYVTSPDTEPDPLKPAGFAPDFGFPNRKERVMVATQQQMDDAQLPLSQRDYCAHYLIKLMKCNRDYWPNFLACKQERHDWDYCEHQDYVMRLKEYERERRLLMRKKRLEETKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVE6</accession>
    <name>W5MVE6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat and SOCS box containing 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVE6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012355"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012376.1">
        <property type="protein sequence ID" value="ENSLOCP00000012355.1"/>
        <property type="gene ID" value="ENSLOCG00000010097.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686691"/>
    <dbReference type="KEGG" id="loc:102686691"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157160"/>
    <dbReference type="HOGENOM" id="CLU_000134_4_1_1"/>
    <dbReference type="InParanoid" id="W5MVE6"/>
    <dbReference type="OMA" id="QHHKITG"/>
    <dbReference type="OrthoDB" id="10069455at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010097">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000016">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF17">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="226"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="28956" checksum="62ED5F19649B58FB" modified="2014-04-16" version="1">MNDFESDWSLLHDAAFNGHLLSLHKLIAKGYSVNLSTLDRVTPLHGACLQGHVLCAKHLIENGANVNVATVDWNTPLFNACSRGNTACVNLLLQKRAEPQGANDLASPIHEAAKHGHTDCIELLVKHGADIDYQVKHLGSPLYVACLGQHLESVTKLLQSGANVNSGKHLDSPLHAAAQNSRPDFVRVLLAHDADVNIRNAEGKQPRDLAPPNSDVEKLLRQGEGPTSLMQICRRSIRKDLGRARLSEVFGLPIPAELKHFLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVG6</accession>
    <name>W5MVG6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVG6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012375"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012396.1">
        <property type="protein sequence ID" value="ENSLOCP00000012375.1"/>
        <property type="gene ID" value="ENSLOCG00000010117.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166785"/>
    <dbReference type="HOGENOM" id="CLU_049505_0_0_1"/>
    <dbReference type="InParanoid" id="W5MVG6"/>
    <dbReference type="OMA" id="HVMLANQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010117">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21980">
        <property type="entry name" value="HMG-box_HMG20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051965">
        <property type="entry name" value="ChromReg_NeuronalGeneExpr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46040:SF4">
        <property type="entry name" value="HMG BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="351" mass="40777" checksum="E7CB63C4756F1752" modified="2025-10-08" version="2">MSSGGQLVPIHPLMSALQPLHNIILDEQQEAKDPDTEEPKSKKRKGGWPKGKKRKPPKEYPAPRAPTTGYVIFVNEKKVQLKMDHPDLPFTEITKMLGVQWSQLSMEEKQRYNNAADRDKQRYVRELKAYQNSEAYKAFLKRKTLNKVKDVCGIECVDSEFENEVFALSQLDGDENGDLFCRTCNQYFSSLHNKKEHLMGKQHLQNLTEEFEKETLGFCKEQEEELEGADDESEEEEEEETDLAHLCGLTSQHSFTSVDISLLEEFLIKQMKLREFELSELKATLDREMEKHENLNKHLQELQRQKINLETELENLKVCGGTLESQMDSLKMVPLFQFHIQVIDRDTPHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW28</accession>
    <name>W5MW28_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chromosome 5 open reading frame 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631919.2">
        <property type="nucleotide sequence ID" value="XM_006631856.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW28"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012587"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012611.1">
        <property type="protein sequence ID" value="ENSLOCP00000012587.1"/>
        <property type="gene ID" value="ENSLOCG00000010282.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690804"/>
    <dbReference type="KEGG" id="loc:102690804"/>
    <dbReference type="eggNOG" id="ENOG502S2NF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000037499"/>
    <dbReference type="HOGENOM" id="CLU_900038_0_0_1"/>
    <dbReference type="InParanoid" id="W5MW28"/>
    <dbReference type="OrthoDB" id="5846619at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010282">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037645">
        <property type="entry name" value="KCT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502:SF0">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17818">
        <property type="entry name" value="KCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="34368" checksum="DAA6F5569501DC40" modified="2014-04-16" version="1">MLSFRCYVRQSPSFSARVMRNWDRASPVYRSKMATCRKMERRYVFYFSVFFFLLPVTCSSNMSDPQGLNTPNNSTSQPPRISVEPEKNMQGSNKDTSSDSAAPRILPTPAPATTVSTTSTPTPAAKTTTTAAHKPSSTSATSAATTPQAGATSSPSAAGRDGTRDDTLLEEPIYDNDDTSPSSPAEDTEDYGEGYQDGEEEGDHDGGEENQVDSIYSVKGSNAMGSRFKDTTIYTPEDEDSHFFFHLVIIAFLVAIVYITYHNKRKIFLLAQRRRWRDGLCSRSVEYHRLDQNVNEAMPSLKMTNDYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWT2</accession>
    <name>W5MWT2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zgc:154054</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWT2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012841"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012867.1">
        <property type="protein sequence ID" value="ENSLOCP00000012841.1"/>
        <property type="gene ID" value="ENSLOCG00000010472.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPQJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156526"/>
    <dbReference type="HOGENOM" id="CLU_027046_3_0_1"/>
    <dbReference type="InParanoid" id="W5MWT2"/>
    <dbReference type="OMA" id="ICHPEKD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010472">
        <property type="expression patterns" value="Expressed in ovary and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF27">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868905" evidence="4">
        <location>
            <begin position="24"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="5">
        <location>
            <begin position="103"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="6">
        <location>
            <begin position="393"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="537" mass="61760" checksum="8FE5AF829E8D5AB7" modified="2014-04-16" version="1">MRCKHGSLLFIVMLASLLSFSWYTNNIRGTGKEIHEELFSITLYQRLQSTEEKSMKLSHELKQVLDHLKFLVQEQKNASSSNQESHTYFKIKNLNKNMLTEIQISNAYIYLPHLKDNENSLLPNVMLGHGRTGVPLVLGIPTVKREKQSYLLNTLSSLLSELSVEEKSDCVIIVFIAEVDEQYVNSVAESIKKNFPNDVESGLLEVVSPSPYFYPNFTNLKETFGDTKERVKWRTKQNLDYSFLMLYAQDKGSLYIQLEDDIVAKPGYSEIMQTFANQQRNNEWLILEFSQLGFIGKMFRTSDLPLIVEFILMFHKDKPIDWLLDHILWVKACNPEKDALHCNRQKDSLRIRYKPSLFQHVGLHSSLRGKIQNLKDKDFGKHTLFIGHPNPPANINTSLSAYQSHTLVRAYQGVDFFWGLTPVSGDYIKIEFHQPLTLKGYRFKSGNIETNGDKFYNTTVEVLPADASVGYKMQKGEMSKYEQTKDGFFRIGAFVNGIAEGEIDPEFNKISAIRLFIHSDSDVWVLLSEVRVFTVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXT1</accession>
    <name>W5MXT1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BRICK1 subunit of SCAR/WAVE actin nucleating complex</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRK1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630743.1">
        <property type="nucleotide sequence ID" value="XM_006630680.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXT1"/>
    <dbReference type="SMR" id="W5MXT1"/>
    <dbReference type="FunCoup" id="W5MXT1">
        <property type="interactions" value="985"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013190"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013218.1">
        <property type="protein sequence ID" value="ENSLOCP00000013190.1"/>
        <property type="gene ID" value="ENSLOCG00000010770.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692606"/>
    <dbReference type="KEGG" id="loc:102692606"/>
    <dbReference type="CTD" id="55845"/>
    <dbReference type="eggNOG" id="ENOG502S3PY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011082"/>
    <dbReference type="HOGENOM" id="CLU_175202_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXT1"/>
    <dbReference type="OMA" id="WEQREFI"/>
    <dbReference type="OrthoDB" id="1883432at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010770">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008064">
        <property type="term" value="P:regulation of actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000017">
        <property type="entry name" value="BRICK1, SCAR/WAVE actin-nucleating complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033378">
        <property type="entry name" value="BRICK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33668">
        <property type="entry name" value="PROTEIN BRICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33668:SF1">
        <property type="entry name" value="PROTEIN BRICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="75" mass="8731" checksum="637B5476A9B4C457" modified="2014-04-16" version="1">MAGQEDPVQREIHQDWANREYIEVITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTKGETLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYT4</accession>
    <name>W5MYT4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 129, E3 ubiquitin protein ligase</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM129 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYT4"/>
    <dbReference type="FunCoup" id="W5MYT4">
        <property type="interactions" value="1670"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013543"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013572.1">
        <property type="protein sequence ID" value="ENSLOCP00000013543.1"/>
        <property type="gene ID" value="ENSLOCG00000011038.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013284"/>
    <dbReference type="HOGENOM" id="CLU_048119_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYT4"/>
    <dbReference type="OMA" id="KFATGPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011038">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018801">
        <property type="entry name" value="TM129"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31322">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TM129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31322:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TM129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10272">
        <property type="entry name" value="Tmpp129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="376" mass="42607" checksum="C75FE5BBF46503B4" modified="2014-04-16" version="1">MEHPEVTFSLAYVVFVVCFVLTPNEFRSAGVTVQNIFSGWLGSEDLGFVQYHIRRTSVTILVHSVLPLGYYLGMCLAAADKNLWRIHQASDEWRIYFAAAVSLPVISGMLVFYWSRNRWGKHPISKTLAAHALPQSSWRAVASSINTEFRRIDKFATGDPGARVIVTDTWVMKVTTYRVHIALQQDIHLTVTDSKQHELSPDSNTPVQMLTISVASVNPRVKPFDISLARLNSVEYGELREKLHAPIRNAANVVIHQTLSDLFLETFKSHVEVNESYSLPSNQDLDPCIGCMQVTANMKLVKLCQDNSDGACQQCYCRPMWCLTCLGKWFASRQDQQHPETWLSSRVPCPTCRARFCILDCCVVRANFRSYEEQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYW8</accession>
    <name>W5MYW8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD repeat domain 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013577"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013606.1">
        <property type="protein sequence ID" value="ENSLOCP00000013577.1"/>
        <property type="gene ID" value="ENSLOCG00000011052.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153859"/>
    <dbReference type="InParanoid" id="W5MYW8"/>
    <dbReference type="OMA" id="MNIPLTC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011052">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000172">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003556">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="487"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="583"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="625"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="667"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="7">
        <location>
            <begin position="802"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="303"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="940" mass="106795" checksum="A3340978A62942EE" modified="2014-04-16" version="1">MGLTKQYLRFAASAVFGVIGSQKANIAYVTLRGGEKGRYVAVAACEHVFIWDVRKAEKVLILKGNKQEVTYLCPSPDGIHLAVGYEDGSIRIFSLLGGESNVSFNGHKSAVTVIRYDELGARLVSGAKDTDVIVWDVINECGLYRLKGHKDAITQALFLKNRNLLVTSSKDTFVKWWDLDTQHCFKTMVGHRSEVWGMVLLNEEKRIVTGSADSELRAWDITYLQENKEEGEPEVKKPKALLDDRDDQEEELDENPAERILSCQKAGSVLRDGRDRVVTMTTDLKSRMLACHGTDAILEVFHVLSEAEVKRRLEKRMKKAKKRAKSLMSRDGKETAEELVERKLEDEIQRLTHIKASAKIRSVQRLGRNTSPTRIAQRLFVWRINHQSDYPINVIPLSFSNMQQDIWTPRDRTRLLRFFESGWVIQSHLEKSFCFFNRSTLQVIRTMACDYALCSLFVPGDRQIVIGTKSGKIQIFDLASGSLLETTDAHNGALWSICLCPDQRGIVTGGADKTVKFWQFELVKDKESGQSKRLTVSHTRTLQLDEDVLCVRYSPDQRLLAASLLDCTVKVFYADTLKFFLSLYGHKLPVLCMDISHDSALIATGSADRNVKIWGLDFGDCHRSLFAHDDSVMFLQFVPKTHLFFTAGKDKKIKQWDADKFEHVQTLEGHHREVWCLAVSPSGDYVVSSAHDKSLRLWERTREPIILEEEKEMAREAEYEESLAKGDEPVVPGETQGEAGLAGKKTIETVKAAERIMEALELYREETRKLEEHSAACSAAGRKLPPPTMSPILVAFGNISPSQYVLDVIRKVRSSELEESLLVLPFSYVPDLLSLFNEFIRGGLEVELVCRCLFFLLRIHFGQITSSQMLLTVIDDLRKNTISRVRQIRDILGFNIAGLQFLQREIENKEDVTFFADATDRFEEKKRKRKKRERAILTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ01</accession>
    <name>W5MZ01_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Ephrin-B2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217324.1">
        <property type="nucleotide sequence ID" value="XM_015361838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ01"/>
    <dbReference type="FunCoup" id="W5MZ01">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013610"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013639.1">
        <property type="protein sequence ID" value="ENSLOCP00000013610.1"/>
        <property type="gene ID" value="ENSLOCG00000011084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685349"/>
    <dbReference type="KEGG" id="loc:102685349"/>
    <dbReference type="CTD" id="30219"/>
    <dbReference type="eggNOG" id="KOG3858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155868"/>
    <dbReference type="HOGENOM" id="CLU_072080_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ01"/>
    <dbReference type="OMA" id="VPYPPKH"/>
    <dbReference type="OrthoDB" id="6250301at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011084">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048514">
        <property type="term" value="P:blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10426">
        <property type="entry name" value="Ephrin-B_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000020">
        <property type="entry name" value="Ephrin-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034255">
        <property type="entry name" value="Ephrin-B_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF18">
        <property type="entry name" value="EPHRIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="18">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="37625" checksum="C4F774F410D8BD29" modified="2014-04-16" version="1">MKAIMRDSTWKYCFGVLIIACKVVLSRTAVLESIYWNTTNSKFIPGQGLVLYPQIGDKMDIVCPRVDSGSGEQVEYYKLYMVPREQVESCSITKADTPLLNCVKPDQDVKFTLKFQEFSPNLWGLEFLRNHDYFIISTSNGTMEGLENQEGGVCQTKSMKIIMKVGQDPNEPISPKDYPTRNPPKHHDGEKDHENEVTNNAEDKPGNGPRTGPNNDKEGQSSSIIGSEVALFAGITSGCVIFIVIIITLVVLLLKYRRRHRKHSPQHTATLSLSTLATPKRGGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZQ7</accession>
    <name>W5MZQ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase</fullName>
            <ecNumber evidence="14">2.4.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-[N-acetyl-alpha-neuraminosyl-(2-&gt;6)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81643"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="Rhea" id="RHEA-COMP:19720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="ChEBI" id="CHEBI:231970"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:81644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-[N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81659"/>
            <dbReference type="Rhea" id="RHEA-COMP:14417"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:139598"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:81660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = a beta-D-galactosyl-(1-&gt;3)-[N-acetyl-alpha-neuraminyl-(2-&gt;6)]-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11136"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:140764"/>
            <dbReference type="EC" id="2.4.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11137"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.3" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01000271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZQ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013866"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013895.1">
        <property type="protein sequence ID" value="ENSLOCP00000013866.1"/>
        <property type="gene ID" value="ENSLOCG00000011284.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689937"/>
    <dbReference type="KEGG" id="loc:102689937"/>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159930"/>
    <dbReference type="HOGENOM" id="CLU_032020_0_1_1"/>
    <dbReference type="InParanoid" id="W5MZQ7"/>
    <dbReference type="OMA" id="TEDHSKY"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011284">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000015">
        <property type="entry name" value="Lactosylceramide alpha-2,3-sialyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941:SF1">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="397" mass="46810" checksum="AD84FDFC05B2D7BF" modified="2014-04-16" version="1">MCQYVMKTTVRKAFFLTLIMSLICLILYGFVFDVKKIYDIRKGDIWSRFLFVEDTLILPPLKRDDFKFMPHSDYDNLYLHDPHHRQTTCPESVMLSKDPEFKKAFIPNIQIFMHKEHFSIKEWNRLIHFNNPFGYMDANYTVLREAVQLIPKLQNYQLLPVPKTDKEGCIRCAVVASGGILNGSRKGKEIDDHDYVFRMNLAVTKGYEEDVGRKTSVYVHSAHALTTAEYFFGANGIPHDEGIRYVMIPEGMRDFQWLTGLLKNSTVATGPYKGFRPWKLYSGQFDPRRFFVLHPDFLRYVRNRFLRASQLNTEDWQIYRPTNGAFTMILALHTCDVVDAYGFITADHAKYPNYYFDKKKTEVIFYLNHDYDLEIRTWKKLHDAKIVNLYQRTEDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZZ8</accession>
    <name>W5MZZ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Family with sequence similarity 83 member Fb</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZZ8"/>
    <dbReference type="FunCoup" id="W5MZZ8">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000013986.1">
        <property type="protein sequence ID" value="ENSLOCP00000013957.1"/>
        <property type="gene ID" value="ENSLOCG00000011357.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692152"/>
    <dbReference type="KEGG" id="loc:102692152"/>
    <dbReference type="CTD" id="566780"/>
    <dbReference type="eggNOG" id="ENOG502QPW1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164021"/>
    <dbReference type="HOGENOM" id="CLU_019056_2_1_1"/>
    <dbReference type="InParanoid" id="W5MZZ8"/>
    <dbReference type="OMA" id="MIENPPQ"/>
    <dbReference type="OrthoDB" id="6103632at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011357">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="3">
        <location>
            <begin position="15"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="543" mass="58693" checksum="2D25B5521C3931E4" modified="2014-04-16" version="1">MADSQLACMDDDHVNEKVPESKPEFYYSEEQRGAVEQLLKNGDGAFRMRIKQDDIKEFLSSREIRRIRETFREYETDGEDKLGSEPPGAAGSKADSGVHSTYWPKMSDTEVPPLDIGWPDTGFYKGVTRVTVHTHPPKENGPHIKAVVRRLIQEARKVLAVVMDSLTDVQIFQDLLDASAKRGVPVYVVLDPAGAPQFTDMCHSLHINGRHLQNLRVRTVEGIGLALSFGKIPGALSSKYMLVDGEKVVSGSYSFTWTASRMDRNLITVMTGQVVDFFDHDFRELYAASLPVDLYKELGVPAPPEPSPAPVKTESRPPTAPATSRFQAMDIHGGPPGNIRAPAHKYHNPKYSLVVGNTLGMSTSCQELAGNGGGSPGIMRRFLQGEKASSERADTVSPLPGPGAQDAEGKSGTKKPGGLSLKKQRSSIRFYLGRGKSGKQSEVAGSNATPPGNATPPGNAGPAPALGRHPLQPSNSSQALEDSFEVVIKPETPLPTPDRKDKRGSKHVSRSVSLRTVSNANGEGIQGSKEAVGRSNKKECILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N021</accession>
    <name>W5N021_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01017451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N021"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013980"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014009.1">
        <property type="protein sequence ID" value="ENSLOCP00000013980.1"/>
        <property type="gene ID" value="ENSLOCG00000011376.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160702"/>
    <dbReference type="InParanoid" id="W5N021"/>
    <dbReference type="OMA" id="WQTEMME"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011376">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05605">
        <property type="entry name" value="STKc_GRK4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF27">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="14">
        <location>
            <begin position="58"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="188"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="451"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="561"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="563"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="593" mass="67813" checksum="83BEB4D5B70B4EDB" modified="2014-04-16" version="1">MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISECEELRKSIDREYYSLCYRQPIGKLLFRQFCETRPEFQRCIKFLNSVVEYEVTPDEKLGANGKEIISTYLSPESSDYIPEISLDLINETKEKHELSPCKELFSHCVNEELSVALGVPVFVVLQTTSHKEHIELGFYLFSRLPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGEAMALNEKQILEKVNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEDRALFYAAEILCGLEDLHREFIVYRDLKPENILLDDYGHIRISDLGLAVKIPEGETIRGRVGTVGYMAPEVLNNQRYALCPDYWGLGCLIYEMIAGQSPFRGRKEKVKREEVDKRVLEIEESYSDKFSEEAKCVCKLLLTKDPKERLGCRGEGASEVKRHPFFKSINFKRLEAGILEPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDQTDDDFYSKFATGSVSIPWQNEMIETECFSELNTFGTIGELPQDLDRSYLPEPPKKGILHRFFRRQHHQNNSNSPSNSKTHSNHHVNSNHVTSNSTGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2K4</accession>
    <name>W5N2K4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="6 14">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="6 14"/>
    <dbReference type="EMBL" id="AHAT01019074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632475.2">
        <property type="nucleotide sequence ID" value="XM_006632412.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2K4"/>
    <dbReference type="FunCoup" id="W5N2K4">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014863"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014892.1">
        <property type="protein sequence ID" value="ENSLOCP00000014863.1"/>
        <property type="gene ID" value="ENSLOCG00000012081.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683317"/>
    <dbReference type="KEGG" id="loc:102683317"/>
    <dbReference type="CTD" id="64841"/>
    <dbReference type="eggNOG" id="KOG3396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008666"/>
    <dbReference type="HOGENOM" id="CLU_072095_1_0_1"/>
    <dbReference type="InParanoid" id="W5N2K4"/>
    <dbReference type="OMA" id="NQRYDWI"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012081">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000031">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="45"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21306" checksum="0846DE5674227906" modified="2014-04-16" version="1">MSPACGMILDDTPLFDPLLLQELDWSANTVPFSPPISPSQPGEGLVLRPLCTADLNRGFFKVLSQLTLASDVTPEQFIKKFEHMKLTGDYYVIVVEDTSLGQIVATATLIVEHKFIHSCAKRGRIEEVVVSDICRGKQLGKLLVSCLTLLSRKLDCYKITLECAPKNVAFYEKFGYSASDETYMQRRFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N374</accession>
    <name>W5N374_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mitogen-activated protein kinase kinase kinase 14a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N374"/>
    <dbReference type="FunCoup" id="W5N374">
        <property type="interactions" value="1051"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015083"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015112.1">
        <property type="protein sequence ID" value="ENSLOCP00000015083.1"/>
        <property type="gene ID" value="ENSLOCG00000012256.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156497"/>
    <dbReference type="HOGENOM" id="CLU_010641_1_0_1"/>
    <dbReference type="InParanoid" id="W5N374"/>
    <dbReference type="OMA" id="IDIWSSC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012256">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000383">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF9">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="222"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="784" mass="87717" checksum="1D3208FDBFF16004" modified="2014-04-16" version="1">KLCCVSIIAQAECECEGKQEFSPTCSEGPFIGSPHCFRSSLDHNNVARTTGHGEGHTSRRKERRKRKRQRQRGERERQRKPSTIPEQESSFHSSFQAQCTGKCASINRKRCGFNDKQGKLWKVSLLPPRGLQSLALSEDGQESPPDSPDSSAAGCRLAVQALRGSVSLGERPYMKPFFQEVKRDACAQGEEGRWGEEEINEGILLNENLKPQNYGYREGEEYSICSHVQNGSFGEVYQIQDKSTGFKCAAKKILLDNFNYEEVGSWSALNSPQIVQLFGAVREGGFVTLFMDLKSGSVGQLLKERGRFPEDRALHYHSQVLEALVHLEKRRVLHRDIKADNVLLSENGEDAFLCDFGHSERLDFKGQYHRAVVGEGLHGTETHMAPEVVRGEPCSAKADVWSSCCMMLHMLNGCHPWTRYYTHPLCLKIANEPPPVNEIPPTCSPYTADVIRAGLEKDPSKRGSAAELKKKVDKALEEVGGLTSPVKGPYQEPASAKPALDSCMNNDPLEPLLTPPSPFRSQQEPLRFMPEHEGSWRRNAKDKKQDKQEEEYKEEFVPELPKSLCAQPVPRPVPQKQESCTTTVPKQEMQQLERAFLMTSLSQAHPPELQEQLLSCLSSDSYSHWDPGDKPQDSGRWSLSVRDDLSSGVFSFNSQTDGHSFSMDTWLGPNSEPPSCFNGVDVLIRDFEGEWLQIREAPKVKVGHIATGISEQISEAAFSLVMSEGDPVHYDDEVCDSGIRLCCVSAPDSGHGWSWRIRDGELETPISPRLCTRLQCQIILHIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4S3</accession>
    <name>W5N4S3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:82559"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Membrane</location>
            <topology evidence="28">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630080.1">
        <property type="nucleotide sequence ID" value="XM_006630017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4S3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015632"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015661.1">
        <property type="protein sequence ID" value="ENSLOCP00000015632.1"/>
        <property type="gene ID" value="ENSLOCG00000012708.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691675"/>
    <dbReference type="KEGG" id="loc:102691675"/>
    <dbReference type="eggNOG" id="KOG3787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155397"/>
    <dbReference type="InParanoid" id="W5N4S3"/>
    <dbReference type="OMA" id="FENDAMN"/>
    <dbReference type="OrthoDB" id="5877963at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012708">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033229">
        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF109">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 28" id="KW-0769">Symport</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="9 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 28" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="520" mass="57293" checksum="84BE7B50D978A1FE" modified="2014-04-16" version="1">MGRKGYNFKMFLKKNWLLLSTVTAVMLGIGLGVLVREYASFSHLDIYYFGFPGEILMRMLKLIILPLIASSVISGVASLDVGLSTKIGLRAITYYLSTTILAVILGIVLVMTIKPGVSQKSEDNDNKSVHEITTLDAILDLLRNMFPENLIQTCFQQYKTHRKELEKPPDKPEQNTTPGSPVTAVMTTAVRENMTEDYTIVGQYSNGINVLGLIVFCLVFGSIIGQMGERGRILRELFEAINEATMKIVKIIMCYMPFGIMFLIAGKIIEVDDWDIFRKLGLYMVTVLCGFAIHGIVCLPLLYFIIVRKNPFTFMLGMAQALVTALMISSSSATLPVTIRCAEENNKIDKRITQFMLPIGATVNMDGSALYEAVAAIFVAQMNDYTLDVGKIITISITSTLASIGAAGIPSAGPVTILIVLSAVGLPAEDVMIIYTIDWLVDRFRTMINVLGDAFGAGIVHELSVKELESNDLTSDRETVSPLDTEPMTDDNECEKKYCINEGFSADKDDSLLHTETSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5D0</accession>
    <name>W5N5D0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongation factor-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5D0"/>
    <dbReference type="FunCoup" id="W5N5D0">
        <property type="interactions" value="1528"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015839"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015869.1">
        <property type="protein sequence ID" value="ENSLOCP00000015839.1"/>
        <property type="gene ID" value="ENSLOCG00000012862.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688296"/>
    <dbReference type="KEGG" id="loc:102688296"/>
    <dbReference type="CTD" id="79631"/>
    <dbReference type="eggNOG" id="KOG0462">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074806"/>
    <dbReference type="HOGENOM" id="CLU_002794_3_1_1"/>
    <dbReference type="InParanoid" id="W5N5D0"/>
    <dbReference type="OMA" id="FARCDIQ"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012862">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor like GTPase 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Elongation factor like GTPase 1"/>
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    </dbReference>
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        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor Tu GTP-binding domain-containing protein 1"/>
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    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056752">
        <property type="entry name" value="EFL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25118">
        <property type="entry name" value="EFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="33"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="460"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="926"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1143" mass="126594" checksum="728C9BEE378006DA" modified="2014-04-16" version="1">MLVKIFENAFNFGQSGMGQTSLDKIIALQKKAANIRNICILAHVDHGKTTLADCLVASNGIISSRLAGKLRYLDSREDEQIRGITMKSSAISLHFSSDGKDYLINLIDSPGHVDFSSEVSTAVRLCDGAIVIVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLITELKLTSQEAYTHLQKMLEQVNAVTGALFTSKVLKERAEKTDALGPCESVSGEQVYDWSSGLDESDDSQLYFSPDQGNVVFASAVDGWGFSIQQFAQLYSQKMGIKTEVLLKTLWGDFYLNAKAKKIMKGAQSKGKKPLFVQLVLDNIWSLYDAVVTRRDKDKIDKMVNSLGLKVSARDSRHTDPRVLLSAICSQWLPVSQAVLTMVCNKLPSPLDIPAERVEKLMCVGVRTFNSLPEKTQELKRAFLECKSEGDTPVIVFVSKMFAVDTKGLPQNKKRPLTQEEIALRREQARRRHAERLAGSQEPDSPEQPETGSGSVPDAAASCGESTKGVDMLSGQMETMALKANRPEEENKEHFIAFARVYSGVVRRGQRVFVLGPKYDPAEASHKLPEGCSAADDLPSVPHLACCTLENLYLLMGRELEELEEVPSGNVLGIGGMEEWILKSATISTSPACPPFTPLNFEATPIVRVAVEPKHPSEMPKLVRGMKLLNQADPCAEVLIQETGEHVLVTAGEVHLQRCLDDLRQRFAKIEIGVSEPIIPFKETVIRPPKVDMVNEDIGKQQKVAVIHQVREEQLKYPEGIQVDSNGLVTLTTSNKLATIGVRAMPLPEEVTHLLEQSSDLIRTMEHFNLSVKEGKKALEINPKMLESIHDFKEKLESLLQGRKWRNAVNHIWTFGPRRCGPNILLNNLEGYQRPSVWQCLDTERVEAGQYRDLDNSIVSGFQLATLSGPMCEEPLMGVCFSVELWDMKAPQVPAAAPPPAQAAPKADSPGPAPHTAGEEALGLQAQLAAPPSKRRSEAAVPDCYGPFSGQLIAAMKEACRYAFQAKPQRLMAAMYTCEIMATADVLGRVYGVLSKREGRVLLEEMKEGTDMFIIKAILPVAESFGFADEIRKRTSGLASPQLVFSHWEVISSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5R9</accession>
    <name>W5N5R9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NEDD4 binding protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000015978"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5N630</accession>
    <name>W5N630_LEPOC</name>
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            <fullName evidence="2">Transmembrane protein 101</fullName>
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        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="258" mass="28852" checksum="4B606E0A1C8A7041" modified="2014-04-16" version="1">QTIMAAPSRKNVLRLLSRFGAIILTRFGFWNCFSMLMLFAERADVNRKPDINMPYLYVDMGAAVLCASFMSFGVKRRWFALGAALQLAVSTYASYIGGQVYYGDWLKVRMYSRAIAIIGGFLVLASGAGEVYRQKPRTRSLQSTGQVFIGIYLICVAYSLQHSQEDRLAYLNHIPGGEITLQLLFVLYGVLALSFLSGYYVHLASQILAILLPLVLLFIDGNVGYWHHDRHVEFWNQMKLIGQNAGIFGAVLILATDG</sequence>
</entry>
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    <accession>W5N7U1</accession>
    <name>W5N7U1_LEPOC</name>
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        <recommendedName>
            <fullName evidence="16">Cell division control protein 6 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDC6-related protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">p62(cdc6)</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
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        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with PCNA, ORC1, cyclin-CDK. Interacts with HUWE1. Interacts with ANKRD17. Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6. Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin. Interacts with TTC4. Interacts (via Cy motif) with CCNF; the interaction takes place during G2 and M phase. Interacts with CDH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217823.1">
        <property type="nucleotide sequence ID" value="XM_015362337.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7U1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016700"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016730.1">
        <property type="protein sequence ID" value="ENSLOCP00000016700.1"/>
        <property type="gene ID" value="ENSLOCG00000013535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693245"/>
    <dbReference type="KEGG" id="loc:102693245"/>
    <dbReference type="CTD" id="990"/>
    <dbReference type="eggNOG" id="KOG2227">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063498"/>
    <dbReference type="HOGENOM" id="CLU_012774_3_0_1"/>
    <dbReference type="InParanoid" id="W5N7U1"/>
    <dbReference type="OMA" id="WPTDEVY"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013535">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000265">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000058">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000547">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="207"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="21">
        <location>
            <begin position="463"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="558" mass="60659" checksum="A8104B6A64C19CA4" modified="2014-04-16" version="1">MPTTRSRSQPTLQYPRRKSSRAAAAKPQPVARAEPPPSGAVLPLSPRKRLGAENGCNLPPGVLGSPPKQSRLRDPSPARGSRRLSFDENVPCRDAPSPVKPAAPSCPAEVPSSPKLGTPQRACTTPTAKGSPARSPFRCRQETPGSSPAGAQGERRPPVVRLFANDNSRFQQVKQALHTSLPERLLSRDRESAVIRSFLCERVKGRRPGSLYISGAPGTGKTACLTRILQDCREELQGAQCVFINCMTLRSSQAVFALVAERLGAAGGQSQPKDSDRRLEKLVTRPGPMVLLVLDEMDQLDSKAQDVLYTIFEWPWLASSRLCLIGIANALDLTDRILPRLQSRPHCRPLLLNFPPYSRQELTAIVQDRLAQVSGEPVLDGVAVQFCARKVSAVSGDARKALDICRRAVEMVESDVRSQKASESKGAPRASLPQVSRVLSEVYGERMAGGEAECFPLQQKLLVCALLLQSQHGKSRELPLGKLHEVYSRLCRQRQVSAVASGECLSLCTLLESRGVFGLKKAKEARLTKVSLKMQEKDVEHALKDKALLGSILAAGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7V5</accession>
    <name>W5N7V5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CST complex subunit CTC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7V5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016714"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016744.1">
        <property type="protein sequence ID" value="ENSLOCP00000016714.1"/>
        <property type="gene ID" value="ENSLOCG00000013558.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RBD3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011553"/>
    <dbReference type="HOGENOM" id="CLU_1681931_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7V5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013558">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029156">
        <property type="entry name" value="CTC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042617">
        <property type="entry name" value="CTC1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865:SF2">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15489">
        <property type="entry name" value="CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="16871" checksum="C16BE9184B63E738" modified="2014-04-16" version="1">PTLCAAHGATQPCFSQVVLCCCVLGPTVCTATLSQLCDTQVVLCVVLLCLRAHSVYCRSVPLNCVTVEQLGNGQSWPADSSGHPSSETPLPIMLLGEWGAGQPRCIMGRVRCHVVCVLFLQLQWVCSLCSSVFKQGRCTRNYPPCHSDSGVFQAEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N932</accession>
    <name>W5N932_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="1109"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000017141"/>
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        <property type="protein sequence ID" value="ENSLOCP00000017141.1"/>
        <property type="gene ID" value="ENSLOCG00000013897.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_026736_0_0_1"/>
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        <property type="component" value="Linkage group LG3"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 3"/>
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            <end position="137"/>
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        </source>
    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAK2</accession>
    <name>W5NAK2_LEPOC</name>
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        <submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006744">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000014344.1"/>
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        <property type="component" value="Linkage group LG1"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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        <property type="entry name" value="FKBP4/5/8-like"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="FK506-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="351" mass="37866" checksum="F5209434A67CDEA1" modified="2014-04-16" version="1">RVALRMQGRGAASSWVSVTPSGWRAVSRRTLERGSGVETPKLGSVCRVRVQSQAADDPGAQADRPTGVDAQERQEDTHTAGLDGSPSSVVQVPLSEWVELRLGEGQCDVIEGCLESMRAGGVCEVIHSPWVCACSSSPWTATRDPRGEWRSGAERLRVELQSFSPGRESWEMSLPEKWAWIRAHKQRGGERFRAGDVWGAADCYGRALRLLATLSRGGAGASGALGSSPDDRGDEDKATEGQSVSEGEYRRVRSELHANMSLCQLKLGQPHKARVSAAQATELDPASTKAWFRLGLACSELGELDAARTGLTRVLELQPGSASALRALRDIEAREKAISCQLGRALSKMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND40</accession>
    <name>W5ND40_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trans-Golgi network integral membrane protein 2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND40"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018549"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018581.1">
        <property type="protein sequence ID" value="ENSLOCP00000018549.1"/>
        <property type="gene ID" value="ENSLOCG00000015074.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693735"/>
    <dbReference type="KEGG" id="loc:102693735"/>
    <dbReference type="CTD" id="10618"/>
    <dbReference type="eggNOG" id="ENOG502S6YU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064712"/>
    <dbReference type="HOGENOM" id="CLU_816263_0_0_1"/>
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    <dbReference type="OrthoDB" id="5846619at2759"/>
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        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015074">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="C:trans-Golgi network"/>
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    </dbReference>
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        <property type="term" value="C:trans-Golgi network transport vesicle"/>
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    </dbReference>
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        <property type="entry name" value="KCT2"/>
    </dbReference>
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        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502:SF0">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17818">
        <property type="entry name" value="KCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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        <location>
            <begin position="24"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
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            <begin position="24"/>
            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
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            <begin position="61"/>
            <end position="104"/>
        </location>
    </feature>
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        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
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        </location>
    </feature>
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        <location>
            <begin position="229"/>
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        </location>
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        <location>
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            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="340" mass="36827" checksum="F550EB4E6C0F1758" modified="2014-04-16" version="1">MCRVVIILSVVWLFLAVASTGNAADVNSRNNQSTTSKGEIGSPSVGQKTSSTSHQDQSQTIDQNAASHVESSTTVQTAVVFSGPSTSANRSGNPSVATKQTQDSVAEKPRTTNIQEQSEQGGNSPEGDKDKTTNPDSVQDPPNKDPQSKSTSVPDKQTTSEPISDTEKTQSTEEKNSGAAPGTKMVTSTEQRHPDTAEKQPEIVSTRTPTAPMTGESQDNDQDKKPTEAQEEGEGDEGEEGEEDEGEVEDKGEKTDDVSVDDSKDNSRLNNQDSSGLQPKDDSESSHFFAYLVSSAMLVAVLYIGYHNKRKIIAYVLEGRRSRTLRRPKSNEYQRLEQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEB6</accession>
    <name>W5NEB6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEB6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018975"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019007.1">
        <property type="protein sequence ID" value="ENSLOCP00000018975.1"/>
        <property type="gene ID" value="ENSLOCG00000015420.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001335_2_1_1"/>
    <dbReference type="InParanoid" id="W5NEB6"/>
    <dbReference type="OMA" id="FIPRTIQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
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            <begin position="328"/>
            <end position="347"/>
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    </feature>
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        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="596" mass="68657" checksum="538463263DC16A89" modified="2014-04-16" version="1">WLDFANAYGSLPHKLIEEAMCHYFIPRTIQDIIKGYLSNIKMRFSVGNSITSWQGLCTISVTLFLMAMNLIIESAQQESRGPNMNSGITVLPIRSFIDDLTVTTTTQVQARWVLRALDTAVKWARMKFKPQKWGSLVIRRGEITDRFGLSVQGEQILSITAERIKCLGKWFDATLKDTNNTREVEKQTLKVWLYQYGLLPRLLWPLMLYEIATTTVEFMERKINSFLEFPLALLASVSSRSSKLQLPILSLVEEFKVANVRLAVGLRVSKDDSIRKAKIQTRSGSKWNVASAVESAESQLKYQKIIGQPTVGRQGLVFSGKYKKKIKESSFNSRRDKKEGRRGSKPIQLGKQETWARWETTDRKLAWKDWKLEPLQIQFLLRSVYDVLPTPSNLHRWGPTESPLNTVWKNRQGRYRWRHDKLLRSLALNLDTFRKGMKPQSARSNISFIKAGEKSTAVAGVRPAGILQRHFSRNWSMEVDLDKRHLFPDIVQTNLRPDVVMSPSQRKVIILIELTVPWEERCQEAFERKKARYAELVDNIRSKGWKVWSFPVEVGCRDFPAPSLWRMFGELGISGKDRNSAVQQMIEAAEKDSCWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEN9</accession>
    <name>W5NEN9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEN9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019098"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019130.1">
        <property type="protein sequence ID" value="ENSLOCP00000019098.1"/>
        <property type="gene ID" value="ENSLOCG00000015518.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159084"/>
    <dbReference type="HOGENOM" id="CLU_027239_1_2_1"/>
    <dbReference type="InParanoid" id="W5NEN9"/>
    <dbReference type="OMA" id="WDKHMDD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015518">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="58"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="35363" checksum="47E47B913BD37759" modified="2014-04-16" version="1">SKINMNKAFQTELTKRMEAAKHMKEEDKLYRYNGLLYPTIMCSPENLKELETFEAREDDIMLAAYPKCGFNWMIGVLHKIVAAATGRSNENPNNHPKFPPMLEFLDPAKQKTLAAAPSPRVVGTHVPPQNFPKSFFEKKTKILVVFRNPKDTAVSFFHFTNGNPVLPTAESWDKFFSDFISGEVPWGSYFDHALAWEEHMDNPNVMVVTYEYLKENLSEAVQQIASFFNCSLSEEQIQTITQESTFKAMKENSKNSHGPMSNVFFRKGEVGDWKNQFSDAQSQEMDAKFEQFLAGTKLGAQLQYDVYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF43</accession>
    <name>W5NF43_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zgc:152830</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01031481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5NF43"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019252"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019284.1">
        <property type="protein sequence ID" value="ENSLOCP00000019252.1"/>
        <property type="gene ID" value="ENSLOCG00000015639.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2597">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063255"/>
    <dbReference type="HOGENOM" id="CLU_013734_0_1_1"/>
    <dbReference type="InParanoid" id="W5NF43"/>
    <dbReference type="OMA" id="ISCFKAP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015639">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:manganese ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine_aapep/pepB"/>
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    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
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        <property type="entry name" value="DIPEPTIDASE B, ISOFORM A"/>
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            <begin position="357"/>
            <end position="364"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00631"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="525" mass="56608" checksum="9A39EB475E35A88F" modified="2025-10-08" version="2">MVRVLRAAGSADCFVTVQPVEYTTDCQDQSQNRLGLKASAPAGVWGSDWTSPAPLSLSAQVDRGLCEEVVVLKVPGLPGNRLVFACTGPVNRDYDDIRRFSDAAVSGVKRAVKAGVQRPLLVCPPHSSYAESPLVAVLGALHALYVPLELREAAVMEKDHKVETLGVWVPEETQGRRITHLATALESGRVVCRDIGGSDPERMAAPRVAQYVQDLFKGTEVKVSVISHIGELQKEYPCLAAVNRCANAVVRHQARVIKLEYCGEGPIENTLLLVGKGITYDTGGADIKAGGVMAGMHRDKCGSAAVAGFFQVLAELKPKHLKVLGAMAMVRNSVGADCYVSDELIVSRAGQRIRVGNTDAEGRMVMADLLCEMKEKAVSEVAPEIFTIATLTGHAIRAMGSDYSIIMDNGEARKAGNAHQWQQAGEVLGDVFEVSMIRRDDFEFHRGKSELDIEYTTIHTLNNCTHYTPEPLVLHIAVSGLLHHGTDSEQPLRYSHIDIAGSSGPFPGIPTGAPILAMATRYVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFG4</accession>
    <name>W5NFG4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01037480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFG4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019373"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019405.1">
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        <property type="gene ID" value="ENSLOCG00000015738.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000290187"/>
    <dbReference type="HOGENOM" id="CLU_1302213_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFG4"/>
    <dbReference type="OMA" id="TEHRAQC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015738">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="23394" checksum="0C3E1777416C5CFF" modified="2014-04-16" version="1">KPRELPPPVTLPCGAPRAAGPPPREARESPAGPPRVLKPASPADRRMSPRCPEPQSPEPRAQSPEPQREPRAPESRVQSPRESPESRAPESRVQSPRESPESQSPRVPESRVPESRVQGSERAQSPRVQSPEPQREPRVQSPRVQSPRVQSPESRVQSPESRAPESRAPESRAPEPQSPRVQSPESRVQSPERAPEPQSPESRAPERAQSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFU2</accession>
    <name>W5NFU2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Scavenger receptor class A member 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SCARA5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SCARA5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFU2"/>
    <dbReference type="FunCoup" id="W5NFU2">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019501"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019533.1">
        <property type="protein sequence ID" value="ENSLOCP00000019501.1"/>
        <property type="gene ID" value="ENSLOCG00000015840.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183074"/>
    <dbReference type="HOGENOM" id="CLU_041152_1_0_1"/>
    <dbReference type="InParanoid" id="W5NFU2"/>
    <dbReference type="OMA" id="LCDEVST"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015840">
        <property type="expression patterns" value="Expressed in zone of skin and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070287">
        <property type="term" value="F:ferritin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000011">
        <property type="entry name" value="Scavenger receptor class A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03070">
        <property type="entry name" value="SCARA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034726">
        <property type="entry name" value="SCARA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF24">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0410">Iron transport</keyword>
    <keyword evidence="5 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="9">
        <location>
            <begin position="86"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="399"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="345"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="424"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="437"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="500" mass="55552" checksum="94843A3DE6E39024" modified="2014-04-16" version="1">MENKVMYMSSYEERENGSFCEERFDGMNSRSLSKLNLCEEASAKLARKKDHCCSHLNSLSAIKYAILSLYILVLLSIFGLCIAVSRLQVSSEKQEALKDNVTLLSERFQNLQLSMSQLSTNNDFLENIWKLENLFQNHSQTLKQLSQAVRRLEQDVHNLQEQSNETTSFVSQLRDVLRDLSFSNQRNVTVLSTDMARVKISLQKQDSRVKEASDQVDNLKDKLEDMQQILNIVNGTFTNDMGIHHTKIQDLQIQIFNVTEDTRTIRVAQINMEQQLRNEVAILNNITEDLRLKDWEHSVALKNLTLVEGPPGPKGEKGDIGALGAPGLPGLPGVRGLIGEKGIQGPRGLKGLAGNDGLPGQKGEPGPKGIEGERGEKGEKGGQGDKGDIGEKTVQEDIIRLVNGSGPHEGRVEVYHEKRWGTVCDDVWDKKDGDVVCRMLGYKGAKEIHKTARFGQGSGMIWMDDVACIGTEDSIQQCRFSGWGKTNCGHVEDAGVTCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI79</accession>
    <name>W5NI79_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related and estrogen-regulated growth inhibitor</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01033536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633693.1">
        <property type="nucleotide sequence ID" value="XM_006633630.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI79"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020338"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020372.1">
        <property type="protein sequence ID" value="ENSLOCP00000020338.1"/>
        <property type="gene ID" value="ENSLOCG00000016464.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686679"/>
    <dbReference type="KEGG" id="loc:102686679"/>
    <dbReference type="CTD" id="85004"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159750"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_7_1"/>
    <dbReference type="InParanoid" id="W5NI79"/>
    <dbReference type="OMA" id="KELCGEY"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016464">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000942">
        <property type="entry name" value="Ras-related and estrogen-regulated growth inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="199" mass="22517" checksum="A071A4E6CA14D66C" modified="2014-04-16" version="1">MAKSAEVKLAVFGRAGVGKSALVVRFLTKRFIWEYDPTLESTYRHQANIDDEVVSMEILDTAGQEDTQQKEGHMRWGDGFVIVYDITDRGSFDEVVLLKNLLDEIRKPKNVTLILVGNKADLEHSRQVSTEEGEKLATEMACAFYECSACTGEGCIAEAFYELCREVRRRKMVQGKTRRRSSTTHVKQAINKMLTKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI98</accession>
    <name>W5NI98_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Visinin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI98"/>
    <dbReference type="FunCoup" id="W5NI98">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020357"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020391.1">
        <property type="protein sequence ID" value="ENSLOCP00000020357.1"/>
        <property type="gene ID" value="ENSLOCG00000016480.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156513"/>
    <dbReference type="HOGENOM" id="CLU_072366_1_0_1"/>
    <dbReference type="InParanoid" id="W5NI98"/>
    <dbReference type="OMA" id="HISRREM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016480">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF101">
        <property type="entry name" value="VISININ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="204" mass="23676" checksum="081E00F2FD1220C6" modified="2014-04-16" version="1">MSLWHGFLEETETMGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLDEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNNDDQITLDEFKEAAKSDPSIVLLLQCDIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIF9</accession>
    <name>W5NIF9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Prolyl endopeptidase</fullName>
            <ecNumber evidence="6">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S9A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01009150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIF9"/>
    <dbReference type="FunCoup" id="W5NIF9">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020418"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020453.1">
        <property type="protein sequence ID" value="ENSLOCP00000020418.1"/>
        <property type="gene ID" value="ENSLOCG00000016532.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063426"/>
    <dbReference type="InParanoid" id="W5NIF9"/>
    <dbReference type="OMA" id="LDPWFSH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016532">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF3">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="8">
        <location>
            <begin position="17"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="7">
        <location>
            <begin position="494"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="722" mass="82234" checksum="5450B26BBD3CC708" modified="2025-10-08" version="2">MSRQSQKLVINMSFQYPEAYRDESVVDDYHGDKIPDPYCWLEDPDSERTKAFVNAQNQLTVPFLEGCPVRNLFKDRMTELYDYPKYSCPFKRGNRYFHFYNTGLQNQSVMYVQDSLDSEPRVFLDPNKFSEDGTIALRGYTFTEDGEYFAYGVSASGSDWVEIKFLRVDGPVELEDRLERVKFSCMSWTHDGKGLFYNSYPIQDGKNTQGTETSTNLHQKLYYHVLGTPQSEDVLCVEFPENPKWMGGAEVSDDGRYVLLSIHEGCDPVNRLWYCDLQEVPEGIKGILPWVKLIDNFDAEYEYITNEGSVFTFKTNLNAPRYRIININFDDPDITNWKELIPEHEKDVIVFASCAHEHFLFVCFLHDVKNVLTMYSLETGEHLKTFLLDVGSVVGFTGRKKDSDIFYYFTSFLSPAIIYHCDLTKDPLEPTVFREVTVKGFDPSDYQTTQVFYPSKDGTQIPMFIVHKKGINMDSSHPALLYGYGGFNISITPSYSVSRLIFVRHLGGVLAIANIRGGGEYGETWHKGGMLAQKQNCFNDFQCAAEYLVKQGYTSSSKLTINGGSNGGLLVAACVNQRPDLFGCAVAQVGVMDMLKFHKFTIGHAWTTDFGCSEIKEQYEWLIKYSPLHNIKVPEENGVQYPAVLLLTADHDDRVVPLHSLKYIATLQYMVSQNPKQTNPLFIHVDTKSGHGAGKPTSKVIQEVADMYAFISRCLDIPWINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIV0</accession>
    <name>W5NIV0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIV0"/>
    <dbReference type="FunCoup" id="W5NIV0">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020559"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020594.1">
        <property type="protein sequence ID" value="ENSLOCP00000020559.1"/>
        <property type="gene ID" value="ENSLOCG00000016642.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004799"/>
    <dbReference type="HOGENOM" id="CLU_031002_1_0_1"/>
    <dbReference type="InParanoid" id="W5NIV0"/>
    <dbReference type="OMA" id="MQLRYHD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016642">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="52"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="468" mass="51064" checksum="9157AEBCB5E7C1D3" modified="2014-04-16" version="1">MTAQPTEFIRGSSTEASRSLFPTLSWEAVFQGGRAGFVTMATSSSSTRPYEIIVFGASGFTGQFVAEEVARTSAEGPKGTLKWAVAGRCRQKLEKVLDQVAETLSKPELKSEVDIIIADVGEPDSLAAMCKQGVIVLNCVGPYRFYGEPVVKACVENGAHCIDICGEPQFLEGMQLNYNKEAAEKGVYIIGSCGFDSIPADMGVLYTRDQFKGTLTAIESFLTVHTGPEGACVHDGTWQSAVYGFADSAKLRSLRRKFGYKPLPVVGSKIKKRYRHVLWLLHDFQSFWGSEASCLLLKEQYQHMSSEEQPVQYGAYAGVGGIGSVVKLMFAGMLFWFLAKFSFGRNLLIKFPEFFSFGVFSKEGPTKKQMEGSSFCFSFIGEGYTEDQDPEQGKPNSKIRIQVKGPEAGYVATPIAMVQAAITLLNEPKSLPKKGGVYSPGAAFSKTNLIERLNKHGIQFVVVGTAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJF5</accession>
    <name>W5NJF5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01035404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJF5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020800.1">
        <property type="protein sequence ID" value="ENSLOCP00000020764.1"/>
        <property type="gene ID" value="ENSLOCG00000016799.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159280"/>
    <dbReference type="HOGENOM" id="CLU_298957_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJF5"/>
    <dbReference type="OMA" id="RFTTNCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016799">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055163">
        <property type="entry name" value="ALK/LTK-like_GRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535:SF3">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12810">
        <property type="entry name" value="ALK_LTK_GRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867837" description="receptor protein-tyrosine kinase" evidence="17">
        <location>
            <begin position="25"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="18">
        <location>
            <begin position="495"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="852"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="943"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="950"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1004" mass="107725" checksum="FF89E0A2F8DB3A57" modified="2014-04-16" version="1">MTRKGLYFFLLTAVFLSEPRCSLLKTSTGAPLSPVLIHSSGEAGGKRDNNQTYFSRLKRKTLSVDFAIPSLFRTYLSELIKRPLNGECLEHDVCYTVRSNLRLHCAPLQKTVSSFLDSKLRAPESAETQADNSSDPGQLPYTRRRPLSRVLNLGLSKVSRKSNQVVIEIGEKVVRAGCGGLRAEEDGPSVPLEMDLSRMLEWWLGAEGGRLRVRLMPERRAQIPGREESYSAAIRAADPRLILQIASRGSDQTAAGDPGDAPGLSRRSHAYWNFTWASEDELQFPLASPQPELHCPDRRPCAQEDYPEFSWSITQAQGLWSDWGGWEEGQFLLLNGSGQWGPWALSPWLWRGGAPCALELAVFLHPQQSGRYTVRLVERHQPPRTILRTDAPRRAGWALVRLALGLSWAPFRISAEYSGHGGHGGQADRDTAALDPRFFTNCTSASPPSPRITLQGRYRCRSGPDISLGQMCDFQTDCPHGDDEGEMCRQFHPGSYCSFEEGDCGWQTTSLSDVSWVVLKSQPGGATDSCASTQGAVLALHGGEAAELRSPLFPPPLRNAPCQVRFRLCQRGHQQRALSLWVVENGTGLEEKQRVWQSGGSTQGWGWSLVSLPLYGVTDWFWLQFSADRGDGFGPAITVDNISLSPECFLASNGDLPPQVTPVTVATVPPEVTNEHLMIDPPEEPVSDPVSMENLIFSTCGATGSRGPTPTQCSNAYRHSKVNVTVVTEGPLRGVQIWRIPATGDYRITGYGAAGGRSVEVTERSHGVYVVAEFLLQKDELLYILVGQQGEDACPNPDPAFNQLCLGESSLTDNKTRTMTAGGGGGGGGATYVFKVVGGVPVPLLIAAGAGGRGYSSGQSKDEEEEELLETDPSVPGQNGRSGAAGGGGGWNDTSPVPWAGQPLVLGALGGQPCPQSLPGWKTRGGFGGGGGACSAGGGGGGYIGGSTSQDDDPTEDGGDGVSFISPEGKAYLMPLKGRTRGPGATCQRLCIVSVTAACALCCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJK2</accession>
    <name>W5NJK2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 38</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC38 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJK2"/>
    <dbReference type="FunCoup" id="W5NJK2">
        <property type="interactions" value="377"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020811"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020847.1">
        <property type="protein sequence ID" value="ENSLOCP00000020811.1"/>
        <property type="gene ID" value="ENSLOCG00000016836.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2610">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002669"/>
    <dbReference type="HOGENOM" id="CLU_029972_1_2_1"/>
    <dbReference type="InParanoid" id="W5NJK2"/>
    <dbReference type="OMA" id="YAFNDVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016836">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd05804">
        <property type="entry name" value="StaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033891">
        <property type="entry name" value="TTC38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263:SF4">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="472" mass="53995" checksum="E0DD4462FE448AF3" modified="2014-04-16" version="1">KTLEMLSASLRDCKAWQSEGLPLSTTSNEACKFYDATLSQYVGWRNDQTLGGIEGCLSKIHAADPNFVMGHVIGTGLELIGTGRSVLLDEKLASAVRKMVELSKSQPLTERERLHVSAVEMFSKGSFPKACLFWEEILMDHPTDMLALKFGHDTYFYMGHQAQMRDSVARVLPHWKPHMPHYSYLKGIYSFGLMETKRYDEAEKVAKEGLALNPGDGWAVHTVAHVHEMKAEVQKGLKFMEQRENYWKECDMLACHNYWHWALYFIEKGEYEDALTIYDNHIARRCLSSGAMLDTVDSCSMLYRLQMEGVMVGDRWKELLNITRPHSEDHVLQFNDLHFLMTSLGAKDESTTRRLVESLRELAKSPGENYQHQMAQDMGLPMCEALVEFDKGNYSRAVELLLPIRYSFDKIGGSDAQRDVFCQLLIHAAMKSENKRHQKLARCLLMERDASRPNSPLTDRLIKKAMTVHNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJU5</accession>
    <name>W5NJU5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="34">Thromboxane-A synthase</fullName>
            <ecNumber evidence="9">1.14.14.1</ecNumber>
            <ecNumber evidence="10">4.2.1.152</ecNumber>
            <ecNumber evidence="33">5.3.99.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="35">Cytochrome P450 5A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Hydroperoxy icosatetraenoate dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="37">Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + AH2 = (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:48856"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:48857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:48636"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:57446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:48637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:55556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59720"/>
            <dbReference type="ChEBI" id="CHEBI:131859"/>
            <dbReference type="EC" id="4.2.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:55557"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde</text>
            <dbReference type="Rhea" id="RHEA:48644"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:90694"/>
            <dbReference type="ChEBI" id="CHEBI:566274"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>prostaglandin H2 = thromboxane A2</text>
            <dbReference type="Rhea" id="RHEA:17137"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:57445"/>
            <dbReference type="EC" id="5.3.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:17138"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 38">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Microsome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 39">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="9"/>
    <dbReference type="EC" id="4.2.1.152" evidence="10"/>
    <dbReference type="EC" id="5.3.99.5" evidence="33"/>
    <dbReference type="EMBL" id="AHAT01023549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023551">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJU5"/>
    <dbReference type="FunCoup" id="W5NJU5">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020904"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020940.1">
        <property type="protein sequence ID" value="ENSLOCP00000020904.1"/>
        <property type="gene ID" value="ENSLOCG00000016908.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684731"/>
    <dbReference type="KEGG" id="loc:102684731"/>
    <dbReference type="CTD" id="6916"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157903"/>
    <dbReference type="HOGENOM" id="CLU_001570_5_2_1"/>
    <dbReference type="InParanoid" id="W5NJU5"/>
    <dbReference type="OMA" id="WPHPETF"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016908">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106256">
        <property type="term" value="F:hydroperoxy icosatetraenoate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004796">
        <property type="term" value="F:thromboxane-A synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000003">
        <property type="entry name" value="cytochrome P450 3A12-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050705">
        <property type="entry name" value="Cytochrome_P450_3A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302:SF47">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24302">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="26" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="14 38 39" id="KW-0349">Heme</keyword>
    <keyword evidence="22 38 39" id="KW-0408">Iron</keyword>
    <keyword evidence="27" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="28" id="KW-0456">Lyase</keyword>
    <keyword evidence="25 40" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 38 39" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0492">Microsome</keyword>
    <keyword evidence="23 39" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="21 39" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="42" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 40" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20 40" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="38">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00013084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00033404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00036475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00038872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00040834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00042726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00047827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00054825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="546" mass="63067" checksum="EE8F84DAB15D36E7" modified="2014-04-16" version="1">MDAAVDILKLVPVKVNVMSVSVSLLVVFLVLLYWYSVYPYSVLARIGINHPKPMPFFGNMFLFREGFWDAHNELIKKYGRVCGYYLGRRPIIVISEPDMIKQILVKDFTNFPNRMHLRLSTKPISESILFLRDERWKRVRSILTPTFSAAKMKEMVPLINIAADTLVENLKSFADSGQSFDVHRCLGSFTMDVIASVGFGTQVDSQRNPDNPFVKHAKQFFSFTFFRPMVMLLIAFPFLISPLIRIVPNKRRDELNNFFVSSIKNMIKHREEQPANERRRDFLQLMLDVKSTKECVSLEDFDVVNQTDVDAPTRTKQYEEPLKKAQKRILTEDEILGQAFIFLLAGYETTSSTLAFACYQMALNMECQKKVFSEVDEFYTRHDTPDYNNVQELQYLDIVISETLRMFPPGFRFARDAAEDCTVNGQFIPRGATVEIPVGYLHYNADYWPEPERFIPERFTAEAKAERHPFAYLPFGAGPRSCVGMRLALLEAKIAMLRIFRRFQFETCPQTEIPLELKSNSTLGPKNGIFLKIIHRQPVACHKEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJW5</accession>
    <name>W5NJW5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member A1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626277.1">
        <property type="nucleotide sequence ID" value="XM_006626214.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJW5"/>
    <dbReference type="FunCoup" id="W5NJW5">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020924"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020960.1">
        <property type="protein sequence ID" value="ENSLOCP00000020924.1"/>
        <property type="gene ID" value="ENSLOCG00000016927.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688288"/>
    <dbReference type="KEGG" id="loc:102688288"/>
    <dbReference type="CTD" id="10559"/>
    <dbReference type="eggNOG" id="KOG2234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182827"/>
    <dbReference type="HOGENOM" id="CLU_024645_1_0_1"/>
    <dbReference type="InParanoid" id="W5NJW5"/>
    <dbReference type="OMA" id="KCYVIAS"/>
    <dbReference type="OrthoDB" id="408493at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016927">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005456">
        <property type="term" value="F:CMP-N-acetylneuraminate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015782">
        <property type="term" value="P:CMP-N-acetylneuraminate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="36753" checksum="08D734B824455070" modified="2014-04-16" version="1">MAHEKVTVLFKMYCLAVMTLVAASYIVVLRYTRTLSSDLYFSTTAVCSTEVIKLILSLGMLAKETGSFGRLKLSITENICGSPKELIKLSVPSVVYAVQNNMAFIALSNLDAAVYQVTYQLKIPCTALCTVLMLQRSLSKLQWFSVFMLCGGVTLVQWEPSVATKVQVEQNPFLGFVAVAIAVLCSGFAGVYFEKVLKSTDTSLWVRNIQMYLSGIVVTLIGVFATDGAAVMEKGFFFGYTPWVCLVIFLSSVGGLYTSIVVKYTDNIMKGFSAAAAIILSTIASVTLFGLQITATFATGTLVVCISIYLYGLPKQDTTKLVRDEVEREAKAKLLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJW8</accession>
    <name>W5NJW8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable arginine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of arginine to tRNA(Arg) in a two-step reaction: arginine is first activated by ATP to form Arg-AMP and then transferred to the acceptor end of tRNA(Arg).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01011930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020927"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020963.1">
        <property type="protein sequence ID" value="ENSLOCP00000020927.1"/>
        <property type="gene ID" value="ENSLOCG00000016928.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063407"/>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="InParanoid" id="W5NJW8"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016928">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="13">
        <location>
            <begin position="462"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="577" mass="65287" checksum="3C0D378360BD8473" modified="2014-04-16" version="1">MACYFRRTIASKLSKVLGSSEESFIAAVSAVPVSKKQQSPDFRVSVSSLVQNGLLQSAGDIQTRTELLGQQIKRDSVVEDVGVGTGVIHFKINRALLAEKVLQEISIKGSDFGVKSELFNQQQKGKKTVVEFSSPNIAKKFHAGHLRSTIIGNFIANLKEVLGNCVIRINYLGDWGMQFGLLGVGFQQFGSEEELKVNPLQHLFDVYVRVNKEAERDENMQEAAREFFRRLETGESGALSLWQRFREISIVEYRRVYERLRLKAMSSLHVNFEENQEQMYVPIICGDCILLALRTGTGVVDLSPAKDMSSFTTVVRSDGTSLYITRDLAAAVDRKNKYDFDEMIYVVGLFTLIYFSPINLRLRVLGARSVASEEQVLLLIIGLRVKTAVTLYIERYLPRHRARPLHSHWEWASGPEMQRSHKKADSDWLFSHIQDFKGPLLSDYRFDWDRVLQSQGDTGVFLQYTHARLRSLLRMCDGGEDRNFDPSHLQDHEATAILQHLLRYDEVLLQSSQDLQPKHLVNFLLTLCRLVAVAHKTLPVKGSPPAVAQARLQLFRSTCIVLASGLRILGITPVEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK10</accession>
    <name>W5NK10_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase 12-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK10"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021005.1">
        <property type="protein sequence ID" value="ENSLOCP00000020969.1"/>
        <property type="gene ID" value="ENSLOCG00000016963.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078084"/>
    <dbReference type="KEGG" id="loc:107078084"/>
    <dbReference type="eggNOG" id="ENOG502QSC4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164581"/>
    <dbReference type="HOGENOM" id="CLU_1140216_0_0_1"/>
    <dbReference type="InParanoid" id="W5NK10"/>
    <dbReference type="OMA" id="RICKRSH"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016963">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056226">
        <property type="entry name" value="WH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057602">
        <property type="entry name" value="Zfn-CCCH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24356">
        <property type="entry name" value="WHD_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25261">
        <property type="entry name" value="zf-CCCH_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="236" mass="27111" checksum="C366635070020883" modified="2014-04-16" version="1">MAVDVTKFLCRNRGCLLYRELKQMMPLSEEQLIEILKDSSKFVIIKGREGTIRGNNLSPDSTIIAKSSLRLCTRYPDCEKCDDLHLCKYFVCGHCFIASGKCRNSHDIHSDHNGAVLRAHFLQDLDEAELFQLLLQNDSYLLPEVCRHYNQGNEEYGSCDYKKNCCKLHVCRYYLQGNCRFGAHCWKSHSFDQNTCLTLKGWGVSEDKIGDLPDIYKNKHRIKEAEEPVSAGVFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKT1</accession>
    <name>W5NKT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein odd-skipped-related 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Odd C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626406.1">
        <property type="nucleotide sequence ID" value="XM_006626343.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKT1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021240"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021276.1">
        <property type="protein sequence ID" value="ENSLOCP00000021240.1"/>
        <property type="gene ID" value="ENSLOCG00000017185.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000021277.1">
        <property type="protein sequence ID" value="ENSLOCP00000021241.1"/>
        <property type="gene ID" value="ENSLOCG00000017186.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697666"/>
    <dbReference type="KEGG" id="loc:102697666"/>
    <dbReference type="CTD" id="130497"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158993"/>
    <dbReference type="HOGENOM" id="CLU_051854_0_0_1"/>
    <dbReference type="OMA" id="CIFCKEE"/>
    <dbReference type="OrthoDB" id="9451254at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017185">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001655">
        <property type="term" value="P:urogenital system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000254">
        <property type="entry name" value="Odd-skipped related transciption factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000090">
        <property type="entry name" value="Odd-skipped-related transciption factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000311">
        <property type="entry name" value="protein odd-skipped-related 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF5">
        <property type="entry name" value="PROTEIN ODD-SKIPPED-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="253" mass="28625" checksum="2F7673637874A9A1" modified="2014-04-16" version="1">MGSKTLAAPVPIHPSLQLANYSLLQASNGFQIPSDHIPNIYGFSALHAVHLHQWTLGYPPMALPRSTFSKLPGLVDGRFQLPAIPIFPHIVQPKQDITNAPVKNKPRFDFANLAVAATQEDHLKAEDLSSAPAIGSLLDVTKLSPERKPSRGRLPSKTKKEFVCKFCGRHFTKSYNLLIHERTHTDERPYTCDICHKAFRRQDHLRDHRYIHSKEKPFKCQECGKGFCQSRTLAVHKTLHMQVKELKPSKIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLL1</accession>
    <name>W5NLL1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger BED-type containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217186.1">
        <property type="nucleotide sequence ID" value="XM_015361700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLL1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021557.1">
        <property type="protein sequence ID" value="ENSLOCP00000021520.1"/>
        <property type="gene ID" value="ENSLOCG00000017415.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687911"/>
    <dbReference type="KEGG" id="loc:102689825"/>
    <dbReference type="eggNOG" id="KOG1121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163186"/>
    <dbReference type="HOGENOM" id="CLU_009123_12_2_1"/>
    <dbReference type="InParanoid" id="W5NLL1"/>
    <dbReference type="OMA" id="CHIPYPC"/>
    <dbReference type="OrthoDB" id="1607513at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017415">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF2">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE ZBED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="692" mass="78829" checksum="376EEE9CD9CB06C6" modified="2014-04-16" version="1">MEIKCSESSPSDLKLVAHPRAKSKVWKYFGFDTDAEGCILHWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPDEFCEFVKSNTEQMREAFATAFSKIKPESSHHFPQDALLMKPSYGYENRKHQDLTLAVVSFICEGMYPVSVVDEPAFKTLLKTADSRYELPSRNYVSTKAIPQKYYLVREALFKELADVVWCAVSTDLWRSETQNRTYISLSSHFLNRCSVNSLVLLTRCLKTFEVPEDNSAENITRALYEAFVEWGINSKVYGATTNCSPDITKACSLLDLPVHMPCFGHTVNKGVEKAFELPKLSTFLRRCRKLVEYFQQSNEAMYMLREKQKLQGLPQCMLISDRVTCWVTTLAMLQRLKEQQFVIASVLVDDSNNHHLMLETTEWSIVDDLVDLLQPFRQAADMMMACKYPTISMVKPLLHMLLNTTLKAKDSDLKEVSMAKEVIAKELSSTYQQTPEIDMFLNVATFIDPRYKKLPFLSPFERSQVENRVIEEAKTVLEKCKEGCAADEPLQFSDEPPVKRQATSSPPPSSHAGNLLAVIFCPSGTDENQEELHAQVMEELSNFKSQKVLGLNEDPLRWWSDRLALFPILPKVLQKYWCVPATSVFPERLFTSSGHVLSGKRNRLAPAHVDQQVFLYENSRNGYEAELAGEDEGEWGIEQDQAIVVGDAFSMQGSFINAKDIGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLY5</accession>
    <name>W5NLY5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Forkhead box B2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626943.1">
        <property type="nucleotide sequence ID" value="XM_006626880.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLY5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021681.1">
        <property type="protein sequence ID" value="ENSLOCP00000021644.1"/>
        <property type="gene ID" value="ENSLOCG00000017539.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686191"/>
    <dbReference type="KEGG" id="loc:102686191"/>
    <dbReference type="CTD" id="442425"/>
    <dbReference type="eggNOG" id="KOG3562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161970"/>
    <dbReference type="HOGENOM" id="CLU_040357_2_1_1"/>
    <dbReference type="InParanoid" id="W5NLY5"/>
    <dbReference type="OMA" id="HPPAQTM"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017539">
        <property type="expression patterns" value="Expressed in brain and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20043">
        <property type="entry name" value="FH_FOXB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000082">
        <property type="entry name" value="forkhead box protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047389">
        <property type="entry name" value="FOXB1_B2_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF215">
        <property type="entry name" value="FORKHEAD BOX PROTEIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="310" mass="34529" checksum="8E7D0AF22670DD7B" modified="2014-04-16" version="1">MPRPGKNSYSDQKPPYSYISLTAMAIQSSQEKMLPLSDIYKFIMDRFPYYRENTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPDCGDMFENGSFLRRRKRFKLMRSDHLASKNSQMLHYFHHQHHQAKLGIGTPETTGGVGRLPQFQTYSINGGQSSGFKHPFAIENIIGRDYKGMMASGLPIASVMHHLGYPVPSQISNMVNSMWPHVGMLDSMAPMPISSDYGPFGVPMKTLCHTTGQTMPAVPLPIKPTPSLGQVSAIPTLTGNAAHLCPSSASSLLEKTLPDMIEGKGNSLHPALMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMY0</accession>
    <name>W5NMY0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMY0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021989"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022028.1">
        <property type="protein sequence ID" value="ENSLOCP00000021989.1"/>
        <property type="gene ID" value="ENSLOCG00000017886.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182934"/>
    <dbReference type="HOGENOM" id="CLU_009579_31_1_1"/>
    <dbReference type="InParanoid" id="W5NMY0"/>
    <dbReference type="OMA" id="TENTHFC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF417">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="192" mass="21681" checksum="8F50D0AA7CF1FA31" modified="2025-10-08" version="2">MNVTGMTENTHFCYDSVNKSCLKHVYVATIRTPLYLILVAVIVFTVCGNLVVIISIAHFRQLHTPTNFLVLSLAVVDLLIGGFIMPPSMVRSLETCWYYGDLFCKIYTTIDVMLCTTSIFHLSFISIDRYYAVCEPLKYQTKITIYVALFMVVISWSVSAVIGFGLIFLELNIKGIEDVYYENVYCVGGCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN16</accession>
    <name>W5NN16_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 51b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
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        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN16"/>
    <dbReference type="FunCoup" id="W5NN16">
        <property type="interactions" value="1025"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022064.1">
        <property type="protein sequence ID" value="ENSLOCP00000022025.1"/>
        <property type="gene ID" value="ENSLOCG00000017922.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_061821_0_0_1"/>
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    <dbReference type="OMA" id="GCAFNTG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
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    </dbReference>
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        <property type="term" value="F:NAD transmembrane transporter activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
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        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052465">
        <property type="entry name" value="Mito_NAD+_Carrier"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER SLC25A51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131">
        <property type="entry name" value="SD08549P"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="183"/>
            <end position="266"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="267" mass="30443" checksum="705A25745D3FE914" modified="2014-04-16" version="1">KHYLCGSCAAVTNIAITFPIQKVLFRQQLYGLRTRDAVRQLQKDGLRNLYRGILPPLVQKTSTLALMFGLYEDFSSLLLRHTSAPELLTRSLAAVLAGTTEAIFTPFERVQTLLQDHKHHHRFNNTFHAFRRLRREHGFREYYRGLGPILLRNGPSNALFFGLRGPIKQCLPEANTRAAHLVNDFICGGVLGAMLGFLFYPLNVVKARMQSQVGGDFPSFRTVLVTIWRERDGKVTHLFRGAHLNYHRSLLSWGIINATYELLLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNT3</accession>
    <name>W5NNT3_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Single-pass membrane protein with coiled-coil domains 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629387.1">
        <property type="nucleotide sequence ID" value="XM_006629324.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199542.1">
        <property type="nucleotide sequence ID" value="XM_015344056.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199543.1">
        <property type="nucleotide sequence ID" value="XM_015344057.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199544.1">
        <property type="nucleotide sequence ID" value="XM_015344058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNT3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022333.1">
        <property type="protein sequence ID" value="ENSLOCP00000022292.1"/>
        <property type="gene ID" value="ENSLOCG00000018191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689983"/>
    <dbReference type="KEGG" id="loc:102689983"/>
    <dbReference type="eggNOG" id="ENOG502RYUI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013440"/>
    <dbReference type="HOGENOM" id="CLU_1229530_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNT3"/>
    <dbReference type="OMA" id="MSTNFHA"/>
    <dbReference type="OrthoDB" id="6112619at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018191">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027895">
        <property type="entry name" value="DUF4533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040004">
        <property type="entry name" value="SMCO3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35972">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35972:SF1">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15047">
        <property type="entry name" value="DUF4533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="215" mass="23517" checksum="71E93BF0E054E5DD" modified="2014-04-16" version="1">MSWSDTFFPANPRRREEVVRLSRELLTLMEGNFRATSWLAGLLNQELKISVDQHKSVKENCEALIAAIAGIQSEVARIDERLRDKLEPALYRRLHNLTVPISERVKVASKVFSSAFGILRTGAAAVPLISKGAVLSRMVASLGRIGSSVVSTVALGVLGLGVDMLVSAILGAVERDNLERVIQEYREALADFRPASEQYQANITDVIATVKAMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNV9</accession>
    <name>W5NNV9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P2Y purinoceptor 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196965.1">
        <property type="nucleotide sequence ID" value="XM_015341479.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196966.1">
        <property type="nucleotide sequence ID" value="XM_015341480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022318"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022359.1">
        <property type="protein sequence ID" value="ENSLOCP00000022318.1"/>
        <property type="gene ID" value="ENSLOCG00000018217.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683577"/>
    <dbReference type="KEGG" id="loc:102683577"/>
    <dbReference type="eggNOG" id="ENOG502QSTF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234621"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NNV9"/>
    <dbReference type="OMA" id="RFLGICF"/>
    <dbReference type="OrthoDB" id="10018446at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018217">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031686">
        <property type="term" value="F:A1 adenosine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045030">
        <property type="term" value="F:G protein-coupled UTP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF17">
        <property type="entry name" value="P2Y PURINOCEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="43"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="37946" checksum="062E2C41CD0DB4EF" modified="2014-04-16" version="1">MMATLNKSESNQSSPYRCSFDESFKYLLLPVSYGLVFLIGMALNCIAMYVIVFRTKRWKPSTIYMFNLIVSDTLYIFTLPFLIYYYAGENDWPFTEPFCKIVRFLFYTNLYGSILFLSCISIHRFLGICYPMQSLQWVSKHRTRLMSVGVWIAVITFQSPILYFSRTTEVGEVNMCYDTTSSELFEDFLVYSSFISIMLFCIPFVVVVVCYGLMVRKLLEPSAGGSCSSKSKQKSIRMIIIVLVVFMVCFLPFHLTRTLYYILRYLGKDISCSALEGASIAYKVTRPLASANSCLDPILYFLAGQGIRKKQSVEKKKLSLKAGTAKLSSTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP56</accession>
    <name>W5NP56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuropeptide Y receptor type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NPY-Y2 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015205143.1">
        <property type="nucleotide sequence ID" value="XM_015349657.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP56"/>
    <dbReference type="FunCoup" id="W5NP56">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022415"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022456.1">
        <property type="protein sequence ID" value="ENSLOCP00000022415.1"/>
        <property type="gene ID" value="ENSLOCG00000018314.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698548"/>
    <dbReference type="KEGG" id="loc:102698548"/>
    <dbReference type="CTD" id="449010"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155973"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_1_1"/>
    <dbReference type="InParanoid" id="W5NP56"/>
    <dbReference type="OMA" id="SEKWPSG"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018314">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000158">
        <property type="entry name" value="Neuropeptide Y receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001358">
        <property type="entry name" value="NPY2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF22">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01014">
        <property type="entry name" value="NRPEPTIDEY2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="70"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="378" mass="42745" checksum="0E4511511BA1E8C5" modified="2014-04-16" version="1">MGPLDLSNSSEGAALVPGSALSTRWHQGSTSSGRTDLHKPGFVTDVTKHLGVQITLIAAYSLIILLGLIGNSLVIYMIVRYRNMRTVTNFFIANLALADLMVDSLCLPFTLVYTLMDEWKFGAVLCHLVPYAQALSVHVSILTLTVIALERHRCIVFHLGHRISKPLSFGIIALTWVAAAILASPLAIFREYRYEEIPFINLKIAVCSEKWPHGSNRDGIIYSLSMLLLQYVVPLGIISYAYVRIWFKLKNHVSPSNRSESVYRRRKTTKMLALVVVVFGVCWLPFHMFQLASDLDLIMKFKDYKLLYTVFHVIAMCSTFANPLLYGWMNKNYRNGFLMFFRCEHKPETIHPEGSFRTRSHRALTLTGPSIGQRPTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP94</accession>
    <name>W5NP94_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="W5NP94"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022453"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022494.1">
        <property type="protein sequence ID" value="ENSLOCP00000022453.1"/>
        <property type="gene ID" value="ENSLOCG00000018352.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160481"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="InParanoid" id="W5NP94"/>
    <dbReference type="OMA" id="SYPYFET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018352">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04116">
        <property type="entry name" value="Rab9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000360">
        <property type="entry name" value="RAB9B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041824">
        <property type="entry name" value="Rab9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF17">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="203" mass="22858" checksum="80D7B454E7DF8618" modified="2014-04-16" version="1">TMSGKSLLLKVILLGDGGVGKSSLMNRYVTDRFDSQSFHTIGVEFLNRDLEVDGRFVTLQIWDTAGQERFKSLRTPFYRGADCCLLTFALDDAQSFRNLGCWKKEFVFYSDVRDAERFPFVVLGNKVDKEDRQVSEEEARAWCQENGGCPYFETSAKDDTNVGEAFEAAVRQVLAAEEQLDHSLLGSAIDLHGNRKPTRTSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVF8</accession>
    <name>W5LVF8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cold-inducible RNA-binding protein B-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVF8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000115"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000115.1">
        <property type="protein sequence ID" value="ENSLOCP00000000115.1"/>
        <property type="gene ID" value="ENSLOCG00000000106.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153524"/>
    <dbReference type="InParanoid" id="W5LVF8"/>
    <dbReference type="OMA" id="CHPEDAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000106">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12449">
        <property type="entry name" value="RRM_CIRBP_RBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000174">
        <property type="entry name" value="cold-inducible RNA-binding protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034278">
        <property type="entry name" value="RBM3/CIRBP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="22847" checksum="84C6FBC6F5873695" modified="2014-04-16" version="1">KDAVIPSWWRRGGRIFKMSDEGKLFVGGLSFDTNEQSLEDVFSKYGTVSEVVVIKDRETQRSRGFGFVTFENPDDAKDAMMAMNGKSVDGRMIRVDEAGKSSGRSGGYRGGSSGGRGFFRGGRGRGGCCYSQGKGWPHGKRSYGGDRSYGGDRSYGSGDRSYSGGYNRSSSGGGGGGYSSGGYYNRDSRGQGSYGDRSGGSYRDGYDSYATHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW39</accession>
    <name>W5LW39_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW39"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000346.1">
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        <property type="gene ID" value="ENSLOCG00000000320.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
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    <dbReference type="InParanoid" id="W5LW39"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
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        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="36508" checksum="8B4C18D7648745AC" modified="2014-04-16" version="1">ESPLNEFLIEPLFARELRTLTIHLLFNHRVPERDMITFLSRFVDVQGEGKKDLDVLKVWTGKRWYTVRLRPKPSECEGVVHPPAYFSIGPNRGYLFYPGQPVTCKKCFQRGHVATNCPGGVCRKCKATIHDTKIKTHRNEEPLRNFYIEPLFARELRPLTVHRFNPYTVEEDILSFLRRFLDVQDEVVKLLDKRKYWTGKRRYRARFRFSAQAADGLLHPPPSFTIGSNKGYLFYLTCRRCGQEGHMALNCVVQICKRCDGIGHIMSACKEVIKCNLCENQGHVYKDCPKRAGSYSMAISKPQRVEEAAPADLVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWC9</accession>
    <name>W5LWC9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein polyglycylase TTLL10-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWC9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000436"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000436.1">
        <property type="protein sequence ID" value="ENSLOCP00000000436.1"/>
        <property type="gene ID" value="ENSLOCG00000000400.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160919"/>
    <dbReference type="HOGENOM" id="CLU_1590205_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWC9"/>
    <dbReference type="OMA" id="DIRLWIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000400">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027752">
        <property type="entry name" value="TTLL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46810">
        <property type="entry name" value="INACTIVE POLYGLYCYLASE TTLL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46810:SF1">
        <property type="entry name" value="INACTIVE POLYGLYCYLASE TTLL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="19885" checksum="2C108E3DE2C57C16" modified="2014-04-16" version="1">MVFFRHGYVRLTCDPYDPSSDDLSAHLTNQYMQKKNPLYNEVKEETVWSMERFNDYVNEKFRAVRALPQDWVLSVFTKRMQQIMTQCFLAVRPKLERKLGYFDLIGCDFMIDEDFKVWLLEMNCNPALHTNCQVLKEVVPSVVNETLDLTIEIFNKCRKKQSLAPLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWT7</accession>
    <name>W5LWT7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWT7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000594"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000595.1">
        <property type="protein sequence ID" value="ENSLOCP00000000594.1"/>
        <property type="gene ID" value="ENSLOCG00000000540.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSRZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271914"/>
    <dbReference type="HOGENOM" id="CLU_013137_8_4_1"/>
    <dbReference type="InParanoid" id="W5LWT7"/>
    <dbReference type="OMA" id="YRGQVEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000540">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002490">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF130">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="32"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="164"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="249" mass="27527" checksum="6804887CC2D27C2A" modified="2014-04-16" version="1">MVSDLSVRFLISEMKSDQSECLCVTLLLLLQSSVSKSESFQVHGPAAPVVVSPGEDAVLPCYLSPSISAVDLEIRWFRGDYNSPVRLYQNLNYNTKIQNPAYRDRAELFLQELPRGNVSLKLTDVRLSDHGQYKCLVESVDHYEDTLISLAVRDVSVSLHSPGGGQTQLLCRSEGWFPAPAVIWTDRDGHDVTSLSSSTVGKDSQGLLSVNSYIPVQQESNIFSCLVRSTQSKADGESQLHIPSKYCIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWW9</accession>
    <name>W5LWW9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWW9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000626"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000628.1">
        <property type="protein sequence ID" value="ENSLOCP00000000626.1"/>
        <property type="gene ID" value="ENSLOCG00000000568.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1ZB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063722"/>
    <dbReference type="HOGENOM" id="CLU_1100624_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWW9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000568">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008677">
        <property type="entry name" value="MRVI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352">
        <property type="entry name" value="LYMPHOID-RESTRICTED MEMBRANE PROTEIN, JAW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352:SF4">
        <property type="entry name" value="LYMPHOID-RESTRICTED MEMBRANE PROTEIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05781">
        <property type="entry name" value="MRVI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="164"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="253" mass="28078" checksum="2F0F98B03A21ED9A" modified="2014-04-16" version="1">MSGSPPRSPPVLQRSHSLSSLPPCRDPWPDWTQGSGNEQNPGTQDTNNTSLPSKKQLRQQAKDQEVHHTSETTAGCRQDPTPSQEVISTMSGQRPSGSVDKERGSGESEEDSDSDDLGTSLDEQSILERLGLNSMQLTEEETESAFAQLSLAFRCDQYTLKRRLQAEERARDIAEENFRQELENGRATLQTLKGLFVDSKRGRIVQKLEQSLEVLSNTIERISSTAELLGAVHQVTAGQQLHMREGHLAYIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZM3</accession>
    <name>W5LZM3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZM3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001580"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001585.1">
        <property type="protein sequence ID" value="ENSLOCP00000001580.1"/>
        <property type="gene ID" value="ENSLOCG00000001388.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155379"/>
    <dbReference type="InParanoid" id="W5LZM3"/>
    <dbReference type="OMA" id="ECIGATK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001388">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070778">
        <property type="term" value="P:L-aspartate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098712">
        <property type="term" value="P:L-glutamate import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAACS"/>
    </dbReference>
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        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF93">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0769">Symport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="633" mass="68622" checksum="607131E75F3EA941" modified="2014-04-16" version="1">MLKERVNLKIRAFQNFPKTPEKSPPAPYSSRLECNPVTVTFTGRLCDTDGLPAHDSEGGYTVLFSQSCANIMPKQVEVRMHESHLEPIEPEATPPRCGGICGKMARNMLLTLTVLGVILGAVAGALLRFASPIPSDIIMVIAFPGDILMRMLKMLILPLIISSLITGLAGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLLIHPGNPKLKARLGEGNKNDEVSSLDAFFDLIRNLFPENLVQACFQQIQTVAKKVPVVPDYSDEENTNSTMDDLLVNATVAPETVYMTKKALEFKGGMNVLGLIGFFIAFGICMGKMGEKAKLMLEFFNILNEIVMRLVIIVMWYSPLGIACLICGKIISIKDLEVVARQLGMYMVTVIIGLIIHGAIFLPLLYFIIVRKNPFTFFIGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGINLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTQDISLLVAVDWLLDRFRTSVNVVGDSFGAGIVYHLSKAELDNLDAQHGHHDDIELTKTQSLYDDLNHRENNSNQCVYAAHNSVLVDECKGISTRCSVVLWAVFGLCAPVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0P6</accession>
    <name>W5M0P6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0P6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001953"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001958.1">
        <property type="protein sequence ID" value="ENSLOCP00000001953.1"/>
        <property type="gene ID" value="ENSLOCG00000001697.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1585919_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0P6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001697">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="17831" checksum="14ABDC82944DA1AC" modified="2014-04-16" version="1">MGVRPSPPLLALMGIRLEGLIPAATLPLLLTMVLFLGPLIQMAMDCPWDFIDGLKVGIVFQFSYTAVFGAYTAFIFIRTGECLTLPCMCVCVCVCVCVWPCCPSLSAPGLYVSLFLSQPLCWVVLCCCAGLSLSPCVGCCVAVRVSLSAPVLGVVWLCGSLPLSQPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0V6</accession>
    <name>W5M0V6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0V6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002018.1">
        <property type="protein sequence ID" value="ENSLOCP00000002013.1"/>
        <property type="gene ID" value="ENSLOCG00000001746.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RY7H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234590"/>
    <dbReference type="HOGENOM" id="CLU_087168_1_0_1"/>
    <dbReference type="InParanoid" id="W5M0V6"/>
    <dbReference type="OMA" id="NERFVMC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001746">
        <property type="expression patterns" value="Expressed in bone element and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF18">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="21824" checksum="30D9D3CC3DCB72C1" modified="2014-04-16" version="1">MCLEQCARASLFPLAALSILANLLLYFPNGETRYASGNQLTNFVWFFGGIIGGGLLVIAPAWMLRGPEGRCCSAREGHVACAEGCPMLSSLVATLFGMTGSLYCLLVSALALTEGPYCISATGWDYPFANSSGSYLFNTTRWSSCKEPARIVVWNASLFSILLGLGATEFIIFFFQLLNTFLLGLCGTCAGCCRQQRYYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M130</accession>
    <name>W5M130_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="9">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01003332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M130"/>
    <dbReference type="FunCoup" id="W5M130">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002087"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002092.1">
        <property type="protein sequence ID" value="ENSLOCP00000002087.1"/>
        <property type="gene ID" value="ENSLOCG00000001784.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT01030000234646"/>
    <dbReference type="InParanoid" id="W5M130"/>
    <dbReference type="OMA" id="PIERRYF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
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    <dbReference type="Bgee" id="ENSLOCG00000001784">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010510">
        <property type="term" value="P:regulation of pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.20:FF:000001">
        <property type="entry name" value="[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.565.10:FF:000007">
        <property type="entry name" value="Mitochondrial pyruvate dehydrogenase kinase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF21">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
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    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="238"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="415" mass="47857" checksum="CE04AC9051E360F9" modified="2014-04-16" version="1">MRLFRLLLKNSTPKQIEYYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLSQPSVRLVQTWYMQSFLELLQYENKRPEDPHVLDDFLDVLIKIRNRHNDVVPTMAQGVIEYKEKYGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGNDTNPAHPKHIGSIDPTCNVAEVVKDAYETAKMLCEQYYMVAPELKIEEFNAKAPQKPIQVVYVPSHLFHMLFELFKNSMRATVELHEGKKEGCPPIKTLVTLGMEDLSIKISDKGGGVPLRKIDRLFNYMYSTAPRPSLEPSRAAPLAGFGYGLPISKLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYQTGPEADDWSNPSSEPRDASKYKANRGRAHTGRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1A8</accession>
    <name>W5M1A8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AlkB homolog 4, lysine demthylase</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AHAT01005628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1A8"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000002170.1">
        <property type="protein sequence ID" value="ENSLOCP00000002165.1"/>
        <property type="gene ID" value="ENSLOCG00000001872.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3959">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006344"/>
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    <dbReference type="OMA" id="MNTLRPC"/>
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        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001872">
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        <property type="term" value="C:contractile ring"/>
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        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
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        <property type="term" value="F:demethylase activity"/>
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    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
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    <dbReference type="PANTHER" id="PTHR12463:SF0">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="271" mass="30501" checksum="D2638A1D0A31394F" modified="2014-04-16" version="1">FMADEEVLQANCACKGIRSCLICENRNGKLDNRINVQQPRRVFVYEPSSGLAVRAEAPGVQESFPFPGVLLWENFVSEAEEQELVSAMDRDAWRDSQSGRRKQDFGPKVNFKKRRVRLGGFSGLPPCSRELVRRMGRAPLLGGFQPVEQCNLDYSPQRGSAIDPHLDDAWLWGERLVTLNLLSPTVLTMSRERAGAGGVWVLVPLPRRALLALYGEARHQWEHAVRRQDIRDRRVCVTFRELSAEFLSGGEQENLGSQLLDVALSFQGIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1K5</accession>
    <name>W5M1K5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GREB1 like retinoic acid receptor coactivator</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GREB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M1K5">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002263"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002268.1">
        <property type="protein sequence ID" value="ENSLOCP00000002263.1"/>
        <property type="gene ID" value="ENSLOCG00000001913.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQXD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008041"/>
    <dbReference type="InParanoid" id="W5M1K5"/>
    <dbReference type="OMA" id="CHQYMEF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001913">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:kidney development"/>
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        <property type="term" value="P:morphogenesis of an epithelium"/>
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    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR028422">
        <property type="entry name" value="GREB1"/>
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        <property type="entry name" value="GREB1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046927">
        <property type="entry name" value="GREB1-like_C"/>
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    <dbReference type="InterPro" id="IPR048657">
        <property type="entry name" value="GREB1-like_cpSF2"/>
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        <property type="entry name" value="GREB1_N"/>
    </dbReference>
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        <property type="entry name" value="TAGT"/>
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    <dbReference type="PANTHER" id="PTHR15720:SF12">
        <property type="entry name" value="GREB1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF20692">
        <property type="entry name" value="cpSF2-GREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20688">
        <property type="entry name" value="GREB1_2nd"/>
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    </dbReference>
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        <property type="entry name" value="GREB1_N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF20691">
        <property type="entry name" value="TAGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GREB1 N-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like second" evidence="9">
        <location>
            <begin position="377"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like circularly permuted SF2 helicase" evidence="11">
        <location>
            <begin position="732"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="domain" description="TET-Associated Glycosyltransferase" evidence="10">
        <location>
            <begin position="1576"/>
            <end position="1800"/>
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    </feature>
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            <begin position="1821"/>
            <end position="1982"/>
        </location>
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            <begin position="60"/>
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        <location>
            <begin position="1177"/>
            <end position="1289"/>
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        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="87"/>
            <end position="98"/>
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    </feature>
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        <location>
            <begin position="245"/>
            <end position="263"/>
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            <begin position="282"/>
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            <begin position="327"/>
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        <location>
            <begin position="370"/>
            <end position="382"/>
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        <location>
            <begin position="1182"/>
            <end position="1196"/>
        </location>
    </feature>
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            <begin position="1201"/>
            <end position="1210"/>
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        <location>
            <begin position="1249"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009148"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF15782"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF20267"/>
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            <dbReference type="Pfam" id="PF20688"/>
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            <dbReference type="Pfam" id="PF20691"/>
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            <dbReference type="Pfam" id="PF20692"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002263.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1982" mass="217552" checksum="0CA19E2161A7B7FE" modified="2014-04-16" version="1">MGNSYAGQLKSARFEEALHNSIEASLRSNNPAPRPVFSQLYLEPEPYASSLEDVKPKVEGLEKEIALHPPERASQELANGQASNSTENEEDMEDEDMSDTSSPPIPYLQRPAPEGSCTLDGFCQAGKDLRLVSVAMEQIEVPPGFELVGAKSPSIPEHILVCAVDKRFLPDDSGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLVKSPQGALCKGPPICWKECKTRQPSSSVSSAKPSSSTSQTAKPDSGASNGPSSPPCLHSDSPPSQTQASSAMSVFGGQDLSRDLGYAKPLALTLPQGSRNPPTAPAAPRAGGSAHGTSSGGRPPPSPSQPPTSGAQPLCSSLGHRAPDAGDPSSPSMSSGPPKKRHRSWYPGSPVPAPPTAVPVPAVRPAVHPSGKVFFSSSPKALLPLVSGVLQPQALPAGDTVIVPDNLLNTSSVRPVILIGYGTLPYFYGNVGDVVVSPLLVSCYKNPPVSYKTLESVGVSVSQLLSVEDMILLTVQYLVRLGPDQIPLREEFEQIALKAMQGERPLQEQGVPQPGPGAASIAPAQLPWLARLAASVSQDSVQVLVTHSSLGEGISESLRSLSEVPPQQRLPDYVVVICASKMRGSEFCVLVLGKYQSRALAESMLTTNEFLKEISYELITGKVSILASHFRATSLGDNLDRQLARFQKRRGDRVTPPFQGDVGDCVESQEAAAMLPDADTGPVGDVFQIYLPQLVAARRLLSQVCSIADSGSQSLDLGRFSKVDFLVLVPPSEVLVHQTAQRIQQSGVLLDLGLEDASSATQKSDKYVVRLDSDVHSKFEAFMRKVKQNPYTLFVLIHDNSHVDLTSLMAGSVSHGELQGLADRVINCREVLSTTNLLVLQVSSFPFTLQTQQSRIGIYNEVHWPAPESLEECGPEEQVYFGLGDYSRSLQWGMASPILRCDDAFEKMVNTLLERYPRLHSMVIRSYLLIQQYTEAMMALVAVSSLRDHATPETLGIVDDLISAPGRDKSGRGHMLLIRVPSLQLAMLARERLEEVRDKLGLQFRFEVMLGSPSAELSLAKHFVARLKTWRGCENEDWVPHTYEELEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACTHVSRGAVQEPLGTMGLSEQELDKAACSEHDPDELHIDLERPQSNGSAVTGTSGSIAENGVSSSSVADSSQRPAGSGSGSGFQKVGSSLEDEGLKQECDSQESQLASNGTAAAPQPSSSSTASSSSSSSSSSQRPSQSTGGGRSSPAAQPRVVILSRAAYKLFVGERAGQMHSFSLLPHADVSWSSPLRPLFHGELGSAQQSLYYRQWTTARQHHADYSAQPGQEGASLHPRRLLLTGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEEVPDVSESTQPTTDQWPDVEAIRKMPFDLLPRDPKFRHSSPVYTDKPNKSLQGYHKEVKQEVPAKRETVSIMLTKYAAYNTFHRCEQCQHYTEATPATQLSDCTFHTFTFSSSMLGEEVQLHFVIPKSKERFFIFSQQGSHLESMVLPLVSDKDPSLLKSPIFTPTTGRHEHGLLNIFHALEGAAHLHILVVKEYEMPLYRKYWPNHILLVLPAMFNSAGVGATRFMIKELSYHNLELERNRLEEQGVKRQDVWPFVIMMDDSCVLWNSHSPQDTGSEAADAGPGFRNVSLKSVIQHAEATPKVTLYAMCGVRKWSSRLRRRGPGTGFSRCHVHDFVLLNVDLTQNVQYDLNRYVCEEVDFNLRVNSSDLLICRFNNFSLMKKHIPFGGHKDLVIKPKLLVSEKLASISPSQYVCAPDSEHMALSAPAQFLLEKFLQHSSYKLFPKAIYNSKNPVLSIDSYLNIGAEVSVFYVSSRPHSVNLDHEGLVFSGLFLYLCDSFVVADLLKKFKFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFRTANGSNERPLYFLTGRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2C2</accession>
    <name>W5M2C2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphodiesterase</fullName>
            <ecNumber evidence="11">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein. In brain, involved in cognitive function, such as learning and long-term memory.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01011610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2C2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002530"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002536.1">
        <property type="protein sequence ID" value="ENSLOCP00000002530.1"/>
        <property type="gene ID" value="ENSLOCG00000002172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165301"/>
    <dbReference type="HOGENOM" id="CLU_032104_1_0_1"/>
    <dbReference type="InParanoid" id="W5M2C2"/>
    <dbReference type="OMA" id="AYQINAQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002172">
        <property type="expression patterns" value="Expressed in ovary and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046069">
        <property type="term" value="P:cGMP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141162">
        <property type="term" value="P:negative regulation of cAMP/PKA signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000006">
        <property type="entry name" value="Phosphodiesterase 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0140">cGMP</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="170"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="501"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="254"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="559" mass="64392" checksum="C5EAA7DF0AF1390A" modified="2014-04-16" version="1">LQMAAKIIYFTVNGRAEQAEFREDCPAQDVKDLFRAAAEAGPYDILKLYNTKGNIINISPGLEPNTPQSCYKLEVVAADCNSVNVTGADLATVLGFDLSSMEKRLQSLEKKILVEAGETPSVVLEMKSQVESFREKLESVEHLSWLGLFKELSEGTHKPSPFYHKRALRKTREECERVRETFLQMSSLEVTEEVKQYLKTPTFDNWQWEDAEIMVLLRVMYTDLDFMTKFNIELEVLQEFLYEVYRRYNNIPFHNFKHCFCVTQMMYGLIWLTDLRSKFDDIDLLTMMTSAVCHDLDHTGYNNAYQINARTELALRYNDISPLENHHCAVAFEILEKTENNIFRNLSTEQYKRIREGMIKCILATDMARHNEILNKFKSILPAFDFTNKDHKDVLMMIMIKVSDISNEARPMDVAEPWLDCLLQEFFNQSDMEKLEGLPVTPFMDRDKVTKPSSQTGFIRFVLLPLFIELANLFPSLEQHIIDPVRKALDYYTEMEKALEKEKQNRAQGDQPGKVQVANTDRPREPAAPSDPKTPANGNLSRPAGPLKPPASTVPYIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2E2</accession>
    <name>W5M2E2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Kinesin family member 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002550"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002556.1">
        <property type="protein sequence ID" value="ENSLOCP00000002550.1"/>
        <property type="gene ID" value="ENSLOCG00000002182.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156463"/>
    <dbReference type="HOGENOM" id="CLU_005295_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2E2"/>
    <dbReference type="OMA" id="CVKKEKF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002182">
        <property type="expression patterns" value="Expressed in embryo and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01373">
        <property type="entry name" value="KISc_KLP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000034">
        <property type="entry name" value="Kinesin family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="34"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="800"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="921"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="628"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="974"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1289"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1384" mass="156356" checksum="7B3CA7AC560DFC13" modified="2014-04-16" version="1">MGTGIKGDSGDAAHLSCKSPLVSGTYFNSGDGDAIKVFVRVRPLTQGTGLSTDGDPSLCLTVTSPNTIRLHSKPEPRVFTYDHVADMDVSQEAVFSSVGKNIVESCVNGYNGTIFAYGQTGSGKTFTMLGPAESDNFTHDMRGVIPRSFEYLFFLINREAEKSRNGKSFLCKCSFIEIYNEQIFDLLDSASASLFLRENIKKGVFVDGAVEKIITSAGEAYQVLSTGWRNRRVASTSMNRESSRSHAVFTVTLESKETVNEIVNIRSSQLNLVDLAGSERQKDTHAEGTRLKEAGSINRSLSCLGHVIMALVDVSNGKSRHICYRDSKLTFLLRDSLGGNARTFIIANVHPGSKCFGETLSTLHFAQRAKLIKNKAVINEDTQGNVRQLQAEVKKLKEQLAQALAAQTPAGARGGPDHEGHSVPAENEHKHRFLEAMLLWEKSESEKKNLLQKVTQLEETWAKKEKFIQSNRMILKFREDHISRLEKSLKESQGGLSSQESEAAVSQLKQEIQILREQVEQHPRVAKYAVENHSLREENQRLRSLEPVKRAQELSAQVAAELEKAFREASAAEKLPGVPPLFSTPVAAENVSSVSVERLRAQLMQKQAELTTTVQAYEEYKELTKKRLLEMESEKQYLEKANQHLENILEATQAHTRQEVSQLNKKHFETIKNLTTPTRGYCLRSRLVPHFSPDHSMDLSARSLPAEDPVDDILNEPVPPEMNELACEALTEELETVQEQVSALQLKLDEEEVKNRKLLQQIGKLEEHSNKMEELLSSERSNWSKEQQNLQDHISSLEKDLREEKTRTDXRRGAGGDLEPLRTRESSFKASRSRLHCSCNSTALLTLFQVTDDGVLFFLILEDLKLEHEKLLEERRSLQDSHDTLQEVMKYESEEHKQRVAESQRENAALQAELSKLLESMKTEKERNSNLTSQLKEDKETTSKELLRALDENVDLKKHISSLTVQSEQQTAELHSLKQSLTAANTSVAELEKKCETEKEFVVDILNQTQELRNTLNEKNEMLAVLSSELEDIKEKYCAALAAREESRALIARHEKEVIDLREAMERRVSSDKIEVSGGPCLLTRKQTSLNGLFFNNLSLKPVAGLELRNEPRDISVVKKKLKQKNELKSPKITSEQNHKAVLPATPLAQTQTPKTPSSFSTDLTKVLEAQEQELENRRSSIVTLEMLVKEFSAERLAQNEEILRLKKAQLCEKENLRLELTDQCYTLQNQMAQNCGSSVEKDGNSHEAVMKELKEERKARVAAMTKLSELEVDLQRTSATLSSAETCIQQMTSELRDRSIELKELREKDLDHEKLAKEVDVLRKQVDFLAEENGKLVGHQNLNQKIQYLVKLKKENTKLIEDNEKLRLENFNLKESCKKIQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4U9</accession>
    <name>W5M4U9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
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    <sequence length="1120" mass="123621" checksum="0D585FA13B60D1A2" modified="2014-04-16" version="1">MPVQAAQWTEFLSCPICYNEFDESVHRPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQTVSLSNVAESKHYEVAKKCVEELALYLKPISGGKGVASLSQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEDALKLVLLALEDGSALSRKVLVLFVVQKLEARFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNCLRPHLELLANIDHNPDAAPPTWDQLENVMLAVKIVVHGLVDFIQNFSKKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGSNCTFAHSQEELEKYRMRNKKISGTVRPYPLPQTVLSKTAGSTAGSDSTAPKLIPRGGDACLEDLKKLARSGSSSLGALSTSPTEPYVSQIKKTGSPPKTPVNHVSATSAIPPSVGADSSSVHSKPGQFVPRVSLYPSQQADVYYQDHRTPYDVASYQQSNNYYTPPPASLQPGQCGTRYVRSSNVPEGSLPPPSVPYPDQYSSFPGRERVGSSAYPPPPPPPPPPRLYGCSMPVFLSTLRCWPPDAHDATAVSLNSGIPAGIHTDFFPCDFRESLNPRPILVFTKVLLVVLQDSYSRMQVGYEELFRRKPEQWAHYHSQKPPPLVSPALPPATQSPSPSSPLFTVDFSTEFSDNSGDYVSSKYKGEDPPSHYSPWSCGTISTCINVIDSEPKDVMANSSVELMHKHLEFLQKRAVLCNTRGETVIILSIKRRMHLFEPHRRLKDEDPIIPFGEGPIISKWGAISRSSRTGYHTTDPIQATASQGSTTKPINFRDYGPYINHSDSRWSSCGSDSSNHSSFLESDQLGVSDLKAQRKSSTTGDTLSTDLQQKRPECCFRCRSPRTEKYLVDDEPERDIELELSALDADEADVQDDKLEDALDCQLRINSHNNHVSNGSAAGNGHQIKQQDKIVMGSHLLKKIAFKKSITPAGGLGSPGLSINELDVKTDPLRAGEGRKRELTHPASNGEMLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4V4</accession>
    <name>W5M4V4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G protein-coupled receptor, class C, group 5, member Bb</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000002905.1"/>
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    <dbReference type="HOGENOM" id="CLU_044162_1_1_1"/>
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    <dbReference type="OrthoDB" id="9882719at2759"/>
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        <property type="component" value="Linkage group LG13"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein kinase activator activity"/>
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        <property type="entry name" value="GPCR_3_C"/>
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        <property type="entry name" value="RA-inducible_GPCR3"/>
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        <property type="entry name" value="G PROTEIN COUPLED RECEPTOR, CLASS C, GROUP 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511:SF9">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY C GROUP 5 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_3"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="8">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="393" mass="42862" checksum="7CB582B8DE1840E4" modified="2014-04-16" version="1">MARCSQWLALFLLLLSLLGGGSSQNSSAPRGCGLGLQASYTPLCDLEAVWGIVLEAVAGAGVLTALLLALVLLGRLPFITEPEKRSPVGLLLLLLLGVLGLFGLSFAYIIRPDERVCVARRALWGVLFSLCFSCLLAQGWRLRRLARQGKSPGAWQLCWLAISLTLVQAIIATEWLVLTVVREGQPACGYLPLDFTLASTYALVLLLASLVLAVLCLCGKFCKWKRNGVFLVLACLLSLLLWAAWSGFYLYGNRALGHPPLWDEPTLAIALVSHGWALLLLHAIPEVHCCVRPPPPPSAPDYFDTSQPPPRLRETSFDEDVALPHRPYMENQAFSFDEHNAALRAGGYRNGNLGSRPSVPFRSNVYQPTEMAVVLNGGTIPSAPPNYTGRHLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4V6</accession>
    <name>W5M4V6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Collagen alpha-1(XX) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Probable collagen protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003414"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003421.1">
        <property type="protein sequence ID" value="ENSLOCP00000003414.1"/>
        <property type="gene ID" value="ENSLOCG00000002883.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153769"/>
    <dbReference type="HOGENOM" id="CLU_002527_2_1_1"/>
    <dbReference type="InParanoid" id="W5M4V6"/>
    <dbReference type="OMA" id="DISGCYG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002883">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01482">
        <property type="entry name" value="vWA_collagen_alphaI-XII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000008">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000234">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000480">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000953">
        <property type="entry name" value="Collagen, type XX, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000638">
        <property type="entry name" value="von Willebrand factor A domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF10">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE ETA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="220"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="422"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="604"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="784"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="95"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1220"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1406"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1225"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1237"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1365"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1416"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1528"/>
            <end position="1542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1542" mass="165834" checksum="9131B44B09E5D0F1" modified="2014-04-16" version="1">MTIQCQGRLKLTVLSEDRLQMKWKEAEGPVQGYRVRVKPLSAETEHEMMLTTSSAKATVAGLVAAQEYTLQIFLLNGTQERLFAKRSFTIDRLREESRNRSEARDTRRKGLGGSGGSTAVNSTQVLQSTATAVRESSEKMQPFVIPLSSADDGPVRKRSKGGKKDRSRTPDAGEKQSHQQAEKENSRKDAPAKTVPTRPETEICLQAKKTFFCETTAPADVVLLVDGSWSIGRTNFKRVRDFLENLITPFNIASDKTRIALSQYSGDPRTEWNLNNFTTKDQLVEAVRNFRYKGGNTFTGQALMHVLKENLQVGSGARLDSAKFVVLLTDGKSQDDAIFAARTLKQAGVEIITVGVKNADEAELKQIASEPVELNVFNVNDFPLLRKLVDKLSRTLCLRIEDKSKAKVTEKPTQAAEETFPGPTNLLFSEVKSREFRMSWSPPSRPVHKYRVVYHSAEGENPQEVVLNGTVSSVLLEGLSSQTEYHISIFPVYDNSVGSGLRGSVTTLPLVPPQYLQIYSTSHNSLRVRWQPAQGATQYMVLYSALADGEPDDAKEVKFGAEQTDVEISGLQPSTDYSITLYALYNEDPSDPITAMAATQPLSPPVKLLFPQVSHSSIRVSWVPASDRVPGHQLTYSTNHGSDVKQVDVSGTTALLLRNLSSLSNYVVAVRSVYEEGLSMPITGNITTLRVPSPSELRVVGFTGSDIKVQWKPAADDVISYLLKWISLSGGKLNQLVVGGGSESAVLEAVEANAEYQISLSALYADGAQSEAVATRYSTFSGGGPSNVVIEGETVNSLLVRWEHPNAHVSEYHVVYTAMKGEPIEETLSVPGKENSVRLRSLLPDTKYSVLVTAMYRNREGGSASAHGKTSSLRVSSLNVYKSEQSSLCVSWKPHGAATGYRIVIQSLKDKTMEEQKLDSSSSSHCFTGLRPEEVYQISVYTQVQQVEGTPVTVLHFTATGPSGPVKLAPPSRSSPRPNEVCPEVTIRNTFLKGFDMMEAFGLTSSAYSSVEGVFAEPFVFNTLPSYSIFRDVQLTQSTSFIHPAGFPPEHTISFTLRLLQDTPKEPFAVWQLTDEDFQPKIGVILDPERKIIQYFSLDFRGDIQEVNFDQAQVRKLFYGSFHKVHLAVSPFSVSLYIDCQPVAESPANSLGTIPTNGFEMLGKLVKSRGPHSGSAPFQLQTFEIICNTSWASEDTCCDIPAKRDEEGCPPPAYACTCTSNIPGSPGPPGPPGKPGPRGERGEKGEQGLKGEAGPPGKLGSRGTSGPLGARGPRGMTVRGRAGPPGERGEKGDIGKPGLQGLPGPPGPKGREGFAGPKGIRGAEGSPGSPGPSGPRGFQGMPGIPGVVGERGHPGQVGPTGLSGHKGEKGEKGRVQEGARSPLLQERWCLSADEVLKLDSFLNEMSQKPVPVEEPVGPPGEPGIPGPQGAPGPRGRPGRPGTTGDPGKSGYPGEQGRSGQPGEKGSVGDSVKGPHGPRGLPGPPGESKPGAPGPRGDPGQPGKPGLSGATGPVGEPGPPGLCDNTGCQRRDPRGETHWGEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M543</accession>
    <name>W5M543_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000002973.1"/>
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        <property type="component" value="Linkage group LG14"/>
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            <end position="853"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5S5</accession>
    <name>W5M5S5_LEPOC</name>
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        <submittedName>
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        </submittedName>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="41015" checksum="64CA0D5E238D3B00" modified="2014-04-16" version="1">RRFEFLIFLFLTLDCIFLAEEVHKVHKLKCILCNFHNGTLTSIPDKDFWNITKLNVSYNNIVIKIQDIETLKKYSKLTELYLNNNNISALPGHVFDSLSELKILNIAYNSISRIEPKAFTGLDKLQELYLCHNQLRSLKAEVFANLTSLKILSLQGNRLESLEEGTFTTLQQATVNLEDNPWNCTCKLIGQLRQLKKHTVKIENQNSTTCVNPKELAVNKSTEIKTTPFPNTPLRSGTSVHPSPFSSLYATTGSNKKPSGTDHPPVGNSWHFLLGVVLIALSTSVVIACAVKSPSWYKLFFNYRHQRLREEDPELFNTSHYSHFGMDTAQLTTNTQDSKTAADDEDGFIEDGYIETADFEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5S9</accession>
    <name>W5M5S9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Complex I-B14.7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase subunit B14.7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA11 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5S9"/>
    <dbReference type="FunCoup" id="W5M5S9">
        <property type="interactions" value="1314"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003737"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003744.1">
        <property type="protein sequence ID" value="ENSLOCP00000003737.1"/>
        <property type="gene ID" value="ENSLOCG00000003164.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6F6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012434"/>
    <dbReference type="HOGENOM" id="CLU_134185_2_0_1"/>
    <dbReference type="InParanoid" id="W5M5S9"/>
    <dbReference type="OMA" id="SIEQGWE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003164">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039205">
        <property type="entry name" value="NDUFA11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21382">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="16657" checksum="22AA685394561F85" modified="2014-04-16" version="1">MGTSGIVKSRDSWCPGALVRGSKMGYWDLQDGKDCVQKTWITTKLSAALGLVGSAYHIIAFQPDSVIKAIERATAATVTMASLGAIFGMTTCLSAQVREKPDDPLNYFIGGCASGIFLGTRTHSYMTGTTACLGLGTLAAFTKIGKMEGWKIAGPPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M717</accession>
    <name>W5M717_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nuclear receptor corepressor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N-CoR nuclear receptor corepressors family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005683">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004175"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004183.1">
        <property type="protein sequence ID" value="ENSLOCP00000004175.1"/>
        <property type="gene ID" value="ENSLOCG00000003513.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155093"/>
    <dbReference type="HOGENOM" id="CLU_000922_0_0_1"/>
    <dbReference type="InParanoid" id="W5M717"/>
    <dbReference type="OMA" id="AVIPPMX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003513">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046966">
        <property type="term" value="F:nuclear thyroid hormone receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000026">
        <property type="entry name" value="Nuclear receptor corepressor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000001">
        <property type="entry name" value="Nuclear receptor corepressor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031557">
        <property type="entry name" value="N-CoR_GPS2_interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF5">
        <property type="entry name" value="NUCLEAR RECEPTOR COREPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15784">
        <property type="entry name" value="GPS2_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="438"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="638"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="639"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="494"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="677"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="985"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1103"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1493"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="1941"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2200"/>
            <end position="2256"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="53"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="76"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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            <end position="1006"/>
        </location>
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            <end position="1017"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1041"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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            <end position="1512"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1678"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1924"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2063"/>
            <end position="2076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2336" mass="261252" checksum="A7F96FE9813F183D" modified="2014-04-16" version="1">MSSSGYPAGQGAFSTEQSRYPPHSVQYTFTTARHQHLQEFAVPDYRPHLQEVSSASQLLQQQQLRRRPSLLSEFHPGTERPPDRRHGTYEQQFHTVSSQSDHEALESKRPRIEAVSEAHFQRAAAAGVLSLTHTLPDGLRASADVKKETTSFNVKTEASSPGPGTQHLVDEQDASPSKLSKEELIQSMDRVDREIAKVEQQIFKLKKKQQQLEEEAAKPVEPEKPVSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHDNIKTNQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVERIENNPRRKAKESRTREYYERQFPEIRKQREQQERFQRVGQRGTGLSATIARSEHEISEIIDGLSEQENNEKQMRQLSVIPPMMYDSEQRRVKFINMNGLMDDPMKVYKARQFMNVWTDHEKEIFKEKFVQHPKNFGLIASYLERKCVADCVLYYYLTKKNENYKTLVRRNYGRRRGRSNHQQITRPSQEEKAEEKTEEDKAEKKEDEEKKEEEEKDEKEESKDIGKDRDKCEGGGEDEEGKEQATPRGRKTANSQGRRKGRITRSMASEAAAVAAEEGPAPPPAPAEPGEPPEHPAARSAPPGPRETRLVEHGRNWSAIAKMVGTKSEAQCKNFYFNYKRRHNLDNLLQQHKQGARRSRGDRDVSQSESVASTISAPEDEENEGSNEEENPEDSEGQFCARKAPPCPSSRQKTAHGGDTRGCRHTAHVFPVPVLTPAAHTPRISDCCTDFVERLVARLSSYCLSCLFHEVFNFTTQHLKIIASEHQRAQLKLSANNIFTEAVEKKKTQSLILVEKVHNKEVKSDKHFIVSAQRIDLGAAGLNFLLSLYSKQQSSQCEVLLVRDRGFRSPPDKEKKFQRMFALDRPSLLSPAGSVLVSSAIKQGHLNVQQLQQRAAMIPPMPLPLTACRATQVSPAFGAAVNPPLSGLAMYQQQITAAVHEASHQEEKQRQDQADPERRSSSCPRTGTKSPSVLDRDAGKPFAYMPYEVKLSMEQDAQSHGNSARSASPYRLSPREFNKASPQPEASRYSVPPVLQPAPNQILPSLPDGVRVTFSRPTRPANIPSPPPLIPSSKPSDKPSFIMGGSISQGTPGTYLPSHSQTVYSQEAAKSSVGSISLGLPRQQDPSKPGDVAVSYIKQEECSPRGQAAQAEGLLSRAQYEGVVRGNPMSAIQEGSITRGTPAKVSAEGLAFRGGSITQGTPALPQSGIPADLLLKGTITKLATEDVGSPEKAREEGLSKGHVIYEGKSGHIVSYDTVKNVREGTRSPRTGHELIGVKRSYEMMEGSITRGHPVRESQVSAPYEGLISRALPREGPHADPKERTVLSGSIMQGTPRATAEAFDEGHKYGKQIKRESPPIRTFDGAITKGKPYEPVTTIKEMGRSIHEIPRQELSGQESRKTPDMAQNTRPIKMYSLNLQPLGSKPNLVLFPVTLRSLYDLXSGKAVSHERKNTLTPTQRDSVSAKSPVSGGDPLAPHSPFDPHHRGVVPGEVYRAHLPPHLDHTIAFHRALDPAATAYLFPRQPSPTGYHNTYQLYAMENTRQTILNDYITSQQMQVIPRPDVARGLSPREQQITIPYPPGARGIIDLAQMPPAILLPHPGGTSTPPMDRITYIPGTQAPFPPRAFNPASISPGHPAHHAVSSASAEREREREREREREQREREKEREKEREREQREREREQRERLFCFLFCYLVSSSRYDAPSGDAVELVSPATSPAQSQEKQDTSFSQEMTKPAQAEAGAWAVTCSLRVAEVERGFVPDPQGPIKHDLHRAVKKPYRFHVLLKHKSASEVLTGCRIDKDLFSRVKIPVLRDMLNQMLKNSLDHKYFHCMCPLKIFRLPSPAWKPEQGKAVLIQDFLGCFAAEQRSFESQMGRYRQQSESPSPQQPPSSQADMYPQVPKTHRVMTLADHISCHPVEVRRLIYRSVNLKFCGVKMSEYFLSGVTALARERKGLLSSSYEPEKRRLRLSYDAERGSDIRTPNTLPELGSVAMDTYEPISPPQSYPSMDKQEAMLQQAQRREADNPEQRSDSRSPGSVSYLPSFFTKLENTSPMVKSKKQEIFRKLNSSGGGDSDMASAQPGTEIFNLPAVTTSGGVSSRSHSFADPASNLGLEDIIRKALMGSFEDKQEEHQGANSQAISMAMGNMTTTMTTMSDARREEANPSPGTGHAAGKQKLAGKANSRKSKSPIPGQGYAGAERPSSVSSVHSEGDYHRQTPAWAWEDRPSSTGSTQFPYNPLTMRMLSTTPPTTIACGPPALQAQPGLLPQSRMWERETTPILMEQYETLSDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M830</accession>
    <name>W5M830_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyridoxal kinase</fullName>
            <ecNumber evidence="9">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively. PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>pyridoxal + ATP = pyridoxal 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:10225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>pyridoxamine + ATP = pyridoxamine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:25105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>pyridoxine + ATP = pyridoxine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:25109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01033653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M830"/>
    <dbReference type="FunCoup" id="W5M830">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004538"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004546.1">
        <property type="protein sequence ID" value="ENSLOCP00000004538.1"/>
        <property type="gene ID" value="ENSLOCG00000003790.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003874"/>
    <dbReference type="HOGENOM" id="CLU_046496_1_1_1"/>
    <dbReference type="InParanoid" id="W5M830"/>
    <dbReference type="OMA" id="AWTHQHP"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003790">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000007">
        <property type="entry name" value="Pyridoxal kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="26">
        <location>
            <begin position="89"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="308" mass="34167" checksum="763C75728DC652D7" modified="2014-04-16" version="1">EMECRVLSIQSHVVRGYVGNKSASFPLQVLGFEVDSINSVQFSNHTGYAHWKGQVLTADELHVLYEGLKLNNVNHYDYVLTGYTRDTSFLEMVVDIVQELKRQNPNLVYVCDPVLGDHGSMYVPENLYPVYKGKVVPVADIITPNQFEAEILCKRALKATEDVLFKVMDLLHAMGPDTVVITSSDLPSSLGVNYLIALGSQKKVKPDGTKVTQRIRMEIPKVDAVFVGTGDLFAAMLLAWTHHHPSDLKTACEKTVSVMHHVLKRTISCAKAMAGDGKKPSASQLELRMVQSKMDIENPEIVIEATVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M873</accession>
    <name>W5M873_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Si:ch211-117m20.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M873"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004581"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004589.1">
        <property type="protein sequence ID" value="ENSLOCP00000004581.1"/>
        <property type="gene ID" value="ENSLOCG00000003836.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S0MW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097129"/>
    <dbReference type="HOGENOM" id="CLU_1717505_0_0_1"/>
    <dbReference type="InParanoid" id="W5M873"/>
    <dbReference type="OMA" id="QCVPCIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003836">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="3">
        <location>
            <begin position="24"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="153" mass="17168" checksum="312C12E78D47EC1B" modified="2014-04-16" version="1">KELKRDRCGDGSCCWLGSKSLCRVNCGKPDADFNALVFGNDWWVGSVVRYACRPGFLLVGDPARACQSDGKWTPKPACMRVCLRGRLEINEKDIDGSCSSTCSAKTHLGPLHHGCVKIDNCVTKQSGWKRWFVRCDYCECDCFTPCELGERRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8Z5</accession>
    <name>W5M8Z5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Carnosine dipeptidase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8Z5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004854"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004862.1">
        <property type="protein sequence ID" value="ENSLOCP00000004854.1"/>
        <property type="gene ID" value="ENSLOCG00000004060.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160484"/>
    <dbReference type="HOGENOM" id="CLU_029469_3_1_1"/>
    <dbReference type="InParanoid" id="W5M8Z5"/>
    <dbReference type="OMA" id="CKGNIVM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004060">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000008">
        <property type="entry name" value="Cytosolic non-specific dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017153">
        <property type="entry name" value="CNDP/DUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF16">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037242">
        <property type="entry name" value="CNDP_dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865818" evidence="11">
        <location>
            <begin position="27"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="12">
        <location>
            <begin position="236"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="10">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="503" mass="56549" checksum="49112017D49EB924" modified="2014-04-16" version="1">VKGKQHSYSSLLFSFHLVRFMMQMACEVTPSVLDPLFQYIDGNQDKYVQRLKKWVAIESESSNSQSWKEVEKMLNVTAGQIRDLGGSVELASVGSQEMPSGESIPLPPVILAEFKKDPTKPTLCVYGHVDVQPAKKEDGWVTDPYNLTEVNGNLYGRGATDNKGPVLAWLHAVETYQALKQEIPVNIKFIIEGMEEVGSDGLETLIRQRNESFFSDVDYIVISDNVWVSRKPALTYGTRGNCYFCVEVEGPKLDLHSGVFGGSVHEPMTDLIALLGSLIEPSGRILIPGINEAVASLTEEEKKLYKGIEFDLEEQKKDMGIPRFLHDSKEEILMSRWRYPSLSIHGIEGDFSDPGLKTVIPRKVIGKFSIRQVPNMDPPVVERQVKEYLQGIFDKHKSPNKLKITMIVGARPWVADLMDPQYVAGRRAIKTVFRVDPDMIREGSTIPIAQTFQEVTGKSVMMLPIGGNDDAEHSQNEKISRFNYIQGTKLFAAYFYELSQLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M941</accession>
    <name>W5M941_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Histocompatibility (minor) 13</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M941"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004900"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004908.1">
        <property type="protein sequence ID" value="ENSLOCP00000004900.1"/>
        <property type="gene ID" value="ENSLOCG00000004095.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156478"/>
    <dbReference type="HOGENOM" id="CLU_023799_0_2_1"/>
    <dbReference type="InParanoid" id="W5M941"/>
    <dbReference type="OMA" id="FLYDIWW"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004095">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043523">
        <property type="term" value="P:regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF23">
        <property type="entry name" value="MINOR HISTOCOMPATIBILITY ANTIGEN H13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="383" mass="42359" checksum="9F1DC4976F24659A" modified="2014-04-16" version="1">MADVDQGSTFSSESASPALDSLNANVSNGTDSTNATVAKFVSTPEGTALAYGSLVFMALLPIFFGALRSVSCAKSKNSSDMPETITSRDAARFPIIASCTLFGLYIFFKIFSQEYINLLLSMYFFVLGILALSHTISPFMNKIFPANFPNKQYQLLFTQGSGENKEEIVNYEFDTKDLVCLCFSSVVGVWYVLKKHWIANNLFGLAFALNGVELLHLNNVSTGCILLGGLFVYDVFWVFGTNVMVTVAKSFEAPIKLVFPQDLLEKGLEASNFAMLGLGDIVIPGIFIALLLRFDVSLKKNTRTYFYTSFFAYIFGLGLTIFVMHTFKHAQPALLYLVPACIGFPVVVALLKGELTEMFRYEESSPLPEVASNEEKETEKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA84</accession>
    <name>W5MA84_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the methyltransferase superfamily. METTL16/RlmF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01005737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA84"/>
    <dbReference type="SMR" id="W5MA84"/>
    <dbReference type="FunCoup" id="W5MA84">
        <property type="interactions" value="1985"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005293"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005301.1">
        <property type="protein sequence ID" value="ENSLOCP00000005293.1"/>
        <property type="gene ID" value="ENSLOCG00000004429.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697714"/>
    <dbReference type="CTD" id="79066"/>
    <dbReference type="eggNOG" id="KOG2912">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016694"/>
    <dbReference type="HOGENOM" id="CLU_027534_0_0_1"/>
    <dbReference type="InParanoid" id="W5MA84"/>
    <dbReference type="OMA" id="TEFCQGH"/>
    <dbReference type="OrthoDB" id="514248at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004429">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052907">
        <property type="term" value="F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120048">
        <property type="term" value="F:U6 snRNA (adenine-(43)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051254">
        <property type="term" value="P:positive regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000062">
        <property type="entry name" value="U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017182">
        <property type="entry name" value="METTL16/PsiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037350">
        <property type="entry name" value="Mtase_ZK1128_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037350-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="567" mass="62260" checksum="B3D37C29ACA8A05D" modified="2014-04-16" version="1">MALNRSMHPRNRYKDKPPDFAYLASKYPEFRQHVHTNLTGRVGLNFKDPEAVRALTCTLLKEDFGLTIEIPLERLIPTVPLRLNYIHWVEDLIGGGSPHRGIDIGTGASCIYPLLGATMNGWYFLATEVDDICYNYAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEETAIVYDFCMCNPPFFANQLEAKGVNSRNARRPPPSSVNTGGVTEIMAEGGELEFVKRIIHDSLQLKRRLRWYSCMLGKKCSLAPLKEELKKQGVPKVTHTEFCQGRTMRWALAWSFCDDVAVPSPPSKKRKLEKARKPITFTVLGPTVRELAAKASSLGSPARGSTDTVTAWIEKTLADLRVLHKRVPCGKSELSLFLTAVENSWIHLRHKRRDRGRQLRELPRAPLEGVGGMAQTPDNPQPAAQSTNSQANPETSARGSEVIGAGNSEEAGVGTCQDCPLPDKNGSSSDRKSSTAEQEKDAAVAQLDVQDSGLDTGAIGVSPGAAGTQEAANASVTAGNSGGPSGVQHFLFKCLMNVKEEGGDVTVEMHWVEGQNRDLMNQLCTYLKNQLFRLVANPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAB5</accession>
    <name>W5MAB5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">pancreatic elastase</fullName>
            <ecNumber evidence="11">3.4.21.36</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.36" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01033735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219037.1">
        <property type="nucleotide sequence ID" value="XM_015363551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAB5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005332.1">
        <property type="protein sequence ID" value="ENSLOCP00000005324.1"/>
        <property type="gene ID" value="ENSLOCG00000004420.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695359"/>
    <dbReference type="KEGG" id="loc:102695359"/>
    <dbReference type="GeneTree" id="ENSGT01030000234528"/>
    <dbReference type="OrthoDB" id="10061449at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004420">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000122">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF0">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865787" description="pancreatic elastase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="28"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="28559" checksum="1315778955148864" modified="2014-04-16" version="1">MFLYIILASIFFVPGHAQEGKNKTSERIIGGIQVTPYSWPWQVSIRISSSDCLPHNCGGVLIQRRWVLTAASCFGSAPLVVVLGEHNIYGIEGKEQFIPVEKKIVHPSWNPNDITAGYDIALLKLTQDAILNTYVNVATLPSSGAILPSTYCYLAGWGRTSTYGQLSATLQQASILIISYSTCSSSSWWGSTVKTTMICAGGDGARSGCAGDGGGALNCYINGRYEVHGVFSFMSSLGCNVSQKPDVFTRISAYIGWINTVIASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBL7</accession>
    <name>W5MBL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myocyte enhancer factor 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBL7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005776"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005784.1">
        <property type="protein sequence ID" value="ENSLOCP00000005776.1"/>
        <property type="gene ID" value="ENSLOCG00000004811.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683003"/>
    <dbReference type="KEGG" id="loc:102683003"/>
    <dbReference type="CTD" id="100271849"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160699"/>
    <dbReference type="HOGENOM" id="CLU_022902_1_0_1"/>
    <dbReference type="InParanoid" id="W5MBL7"/>
    <dbReference type="OMA" id="TAFMSPH"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004811">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="425" mass="45959" checksum="0BE0ED8B8B37BA64" modified="2014-04-16" version="1">MGRKKIQISRIVDQRNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNRLFQYASTDMDKVLLKYTEYSEPHESRTNADILETLRRKGLGLDGSELESEEVMSAVATEKYQQLNEGMDLSITRQRYYAPPLLPSEAQYLVSGACENGFSPGNPASSGLGSHRPPPSKPPGPKPGIAGGWGSHSQPPLMPPHAGIGYSMFSHGHLSRTLAKTPPPLNLGSDGRRMEGHPGLTGTRSLGTARVLYPGVPAGNHVVTMGKTGLLGHSLGGYGLSSAGPPEYPHPGFSHSVTLQRGAISPWQQEPHHVAHSSLGMSSSGCSFSAHTSAPTPPPHPSLNLSIKSERVSPVHTCSSATPPLQHVAQHSPISNPDSTGHAPQDPYATNEREDYQKGGYISPQVLCRAHPEDKQGPPSRRSEIAENWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBR8</accession>
    <name>W5MBR8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">N-acetyl glucosamine (GlcNAc) transferase that catalyzes the transfer of GlcNAc via a beta1-&gt;3 linkage from UDP-GlcNAc to the non-reducing terminal galactose (Gal) in the linearly growing chain of N- and O-linked keratan sulfate proteoglycans. Cooperates with B4GALT4 galactosyltransferase and CHST6 and CHST1 sulfotransferases to construct and elongate mono- and disulfated disaccharide units [-&gt;3Galbeta1-&gt;4(6-sulfoGlcNAcbeta)1-&gt;] and [-&gt;3(6-sulfoGalbeta)1-&gt;4(6-sulfoGlcNAcbeta)1-&gt;] within keratan sulfate polymer. Involved in biosynthesis of N-linked keratan sulfate proteoglycans in cornea, with an impact on proteoglycan fibril organization and corneal transparency. May play a role in the maintenance of tissue architecture by suppressing cellular motility and invasion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01015178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216540.1">
        <property type="nucleotide sequence ID" value="XM_015361054.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBR8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005827"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005835.1">
        <property type="protein sequence ID" value="ENSLOCP00000005827.1"/>
        <property type="gene ID" value="ENSLOCG00000004855.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695683"/>
    <dbReference type="KEGG" id="loc:102695683"/>
    <dbReference type="CTD" id="93010"/>
    <dbReference type="eggNOG" id="KOG2287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157606"/>
    <dbReference type="HOGENOM" id="CLU_036849_5_1_1"/>
    <dbReference type="InParanoid" id="W5MBR8"/>
    <dbReference type="OMA" id="MFQWKKT"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004855">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018146">
        <property type="term" value="P:keratan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000014">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF93">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="408" mass="47456" checksum="FFE524311180D7D2" modified="2014-04-16" version="1">MLTTRDRWKIYKTACLMFFVAVITLTVLQRGSTYSGAQFREDSPSKQESLSEGVHHMQKKGLLPGTEGFWKASKQASATEKPRVTASRAPRTWDVTTTNCTANFNFTQMEWFGGLESNFKQFLLYRHCRYFPMTINHPEKCKGEIHLLIVVKSIITQYDRREVIRKTWGREKEANGKRIKTLFLLGAPSKEEEKANHQKLLEYEDYKYGDILQWDFMDSFFNLTLKEVHFLKWFSTYCENVHYIFKGDDDVFVSIENIVEFLDGSKIRNLFVGDVLYKAKPIRKKENKYYVPQALYNKTHYPPYAGGGGFLMDGPLARRLFGACETLDLYPIDDVFLGMCLEVLRVTPVKHNAFKTFGLVKNKSSKLNREPCFFKSMIVVHKLLPPDLMRMWKLVNSNLVCSRKVNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC96</accession>
    <name>W5MC96_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Apoptosis antagonizing transcription factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC96"/>
    <dbReference type="FunCoup" id="W5MC96">
        <property type="interactions" value="1189"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006005"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006013.1">
        <property type="protein sequence ID" value="ENSLOCP00000006005.1"/>
        <property type="gene ID" value="ENSLOCG00000004972.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000288"/>
    <dbReference type="InParanoid" id="W5MC96"/>
    <dbReference type="OMA" id="INFMAPN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004972">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="4">
        <location>
            <begin position="216"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="3">
        <location>
            <begin position="465"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="557" mass="63789" checksum="40A5996810E4D4FB" modified="2014-04-16" version="1">MAASISQQLEDLLNPLPKFLDPEDDQDEETKAKVIEKFDEGENEDGEAAVSGLRQRASALLAETDRRYLGRATSRKELRRDAESSDSCSSCGIRDWLLTPRFCTGEEEEEEELQEGASEEEDEEEEDSDLSDDDGTQMKALLREKTDDFVFPKETDFRKLAEGMEEEGESEEEEEEEMEGEEEEEEMEGDEEEEGMEREDDEDAVMTFSKEKVSEEVEKGKAVKNQLVLWDQLLEGRIKLQKALLSANQLPQPQTFPLFKKRGGAEFAGALKNSHKALKALQRSLLELQDQLLCQHPDTRAIVLGKTGAARHSEDDDEEEEEEEVPSEEEEGGEKRAWAGPPKRKLEMEEYPEVMAKRFAAFQPYRDSTLQKWHDKTRLTMGKMGKGFSAFERNILTQIEHVLLDRGRLLQRTQTRRSDYRVLGRPEPKIGTGNEQLGQEEEPALKANAHLKDLDEEIFDDDDFYHQLLRELIERKTSEVDPNDQVAMGRQWLAIQRLRSKIKRKVDTKASKGRKVRFHAHSKLVNFMAPVDHGAMNDDARSRLIRSPFVTGIKTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCZ6</accession>
    <name>W5MCZ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01013494">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCZ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006255"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006263.1">
        <property type="protein sequence ID" value="ENSLOCP00000006255.1"/>
        <property type="gene ID" value="ENSLOCG00000005196.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014852"/>
    <dbReference type="HOGENOM" id="CLU_019666_1_1_1"/>
    <dbReference type="InParanoid" id="W5MCZ6"/>
    <dbReference type="OMA" id="GWLKPYN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005196">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015929">
        <property type="term" value="F:hexosaminidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06565">
        <property type="entry name" value="GH20_GcnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038901">
        <property type="entry name" value="HEXDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040">
        <property type="entry name" value="BCDNA.GH04120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040:SF5">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="6">
        <location>
            <begin position="153"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="575" mass="65747" checksum="0A2CB3FB729F9B72" modified="2014-04-16" version="1">MRKPAVLRLVVLSVVVLTAVRFLLRSNVGMVLSIIERGHREIGGGAVLNEKQTPQKKEALVWARQQTSQSQEPQKSQGGEAQDSPKKPKVTPRQDTNQTPLRVVHFDLKGAAPKLTYLEQVFPLLSSLGANGILLEYEDMFPYEGDLQILRSPHAYSVEDIQKIRHLAELSKLEIIPLVQVFGHLEFALKHEKFSNLREVPGFPNSLNPHAPGSQPLVQAMLSQVLDRHPGLRWLHIGADEVFGLGQGLDSKNWLNSNNGDMGRMFLNHVREIVHFATERHPGLRLLMWDDMMRHISVDTLKASSLTSFVSPMIWDYGADLQINQIEKFISNYQAAGFEGVWFASAFKGASGVAQDWTPLTQHMKNHLSWLKVMAVMPKYPSIHFQGIALTGWQRYERYDHFTVLCELFPVGIPSLAVCLQSLAHGGFNEEAKKQVLHMLGCKNINLEKSLCCSEGGGAFPGYEVYQMVLHIRENLKSQIHQQFDNQHLKGWFSRYHRKYKFGNPRNMEFFKDKMSKLLEEWEDYLQNFRTQMEAIYFADTVEEWMEENVNEHMDKLRELAADFEQVIKSRGGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD07</accession>
    <name>W5MD07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01028901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006266"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006274.1">
        <property type="protein sequence ID" value="ENSLOCP00000006266.1"/>
        <property type="gene ID" value="ENSLOCG00000005202.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155466"/>
    <dbReference type="InParanoid" id="W5MD07"/>
    <dbReference type="OMA" id="TAGQHRE"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005202">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08691">
        <property type="entry name" value="C2_NEDL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000036">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2840:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000048">
        <property type="entry name" value="HECT, C2 and WW domain-containing E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000035">
        <property type="entry name" value="LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037795">
        <property type="entry name" value="C2_HECW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040524">
        <property type="entry name" value="HECW1_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032348">
        <property type="entry name" value="HECW_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF127">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18436">
        <property type="entry name" value="HECW1_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16562">
        <property type="entry name" value="HECW_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="168"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="790"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="967"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="1219"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="26"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="327"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="434"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="663"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1014"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="554"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1554" mass="172994" checksum="A019E7DC13278ED1" modified="2014-04-16" version="1">MAASAREHLLFVRRRSPQMRYTLSPENLQSLAEQSSGPPEPMGLQRANSDTNLVTSDSRSSLTASTYEFTLGLAQNLIISWDIKEEVDATDWIGLYHIDETCPANVWDSKNRGVNGTQKGQIVWRLEPGPYFMEPETKICFKYYHGVSGALRATTPCITVKNPGILVGAEGLAEGESVAEHCRKLVSFTLSDIRALGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIIANTTNPTWHGERYTFVALVTDVLEIEVKDKFAKSRPIIKRFLGKLIIPVQRLLERYAVGDQTLSYTLCRRLPTDHVSGQLQFRVEITSTGHEDASPDTMGSLLGATAVNGDPGSPSDDEDLPGGPHPHSQPVPGTTREPSPTGSEGSVPGEVLRNGEGQEEDETTREGVEGGAEGGFQLRCTHALRQESLNDYLDAIEAPKGPGERPLGAASPKLRSSFPTDTRLSAMLHIDSDEDEEVGLRPEPGGETEGGLLLLLNGGLEGKPPRTEGGEVEAVQPIEEIEGTAVAVAQLPEEGEPPSHSPPGPDVQEGPEEPEPGGSSQEQESEVTEGGPSSGTGTTRQSSTGCSSFDSSRYPASSSQVSGPPAGREGAGNEALDTTGRADPDFLNENCLELMWFADFSLGSSYRCSVVRSMRYPASFAHSFEEEEGGAPSSDPGGGGSSEATPTTSDVADEGGGSGQPAVGGEDPQGATAANGDEEEEEIWRRRQSLQAAGGESQEEDVRSAGASGPVDSGQSATETDGDAASQVNGHQPMRSLPSVRQEISRYQRVDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEDQASEIPPEDGDLLHHTIPEYRRDGMLPPSSTRSRLSLLLQSPSAKFLTSPDFFTVLHSNPSAYRMFTSNSCLKHMISKVRRDAHHFERYQHNRDLVAFLNMFANKQLELPRGWEMKHDHTGKPFFVDHNCRSTTFIDPRLPLQSARPSSLLAHRQHLTRQRSHSAGEVGDDSRHSGPPVLPRPSSTFNTVSRSQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPELIRNHSLREKVQFIRSEGAPGLARLSSDADLVMLLSLFEEEVMSYVPPHALLHASYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEYLDEEFHQSLQWMKDNDINDMLDLTFTVNEEVFGQITERELKPGGANIPVSEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITSLPRAHTCFNRLDLPPYPSFSMLYEKMLTAVEETSTFGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD71</accession>
    <name>W5MD71_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zona pellucida-like domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cupulin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Glycoprotein which is a component of the gelatinous extracellular matrix in the cupulae of the vestibular organ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD71"/>
    <dbReference type="FunCoup" id="W5MD71">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006330"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006338.1">
        <property type="protein sequence ID" value="ENSLOCP00000006330.1"/>
        <property type="gene ID" value="ENSLOCG00000005256.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687002"/>
    <dbReference type="KEGG" id="loc:102687002"/>
    <dbReference type="CTD" id="560177"/>
    <dbReference type="eggNOG" id="ENOG502QQQ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156527"/>
    <dbReference type="HOGENOM" id="CLU_045259_2_0_1"/>
    <dbReference type="InParanoid" id="W5MD71"/>
    <dbReference type="OMA" id="FLMPVCG"/>
    <dbReference type="OrthoDB" id="9274484at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005256">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF24">
        <property type="entry name" value="ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865983" description="Zona pellucida-like domain-containing protein 1" evidence="15">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="16">
        <location>
            <begin position="43"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="415" mass="45399" checksum="A97F7156AB826D16" modified="2014-04-16" version="1">MEQICLVILLIGKALSVGAQFNGYNCDANHHSRFPAERDISVYCGVQTITLKINFCPVLFSGYTDADLALNGRHGDAHCRGFINNNTFPTVVLFTISLNTLESCDNSLVVSSAQGMNAFGNLSLVQIGNISGYIDTPDPPTIISYLPGLLYKFSCSYPLEYLVNNTQLASSSAAISVKENNGTFLSTLNLLLYNDSTYAQQLSIPMAGLTLKTKVFAAVKATNLDRRWNVMMDYCYTTPSGNPSDELRYDLFFSCDKDPQTTVIENGKSQMGRFSFEVFRFVKHKNQKMSTVFLHCVTKLCKADDCPLLMPICGSRKKRDLSEKVTPKPQSTSGNAVITAGPIITRSDETPTNNSQLAQLNGPAFQLNSVTSALISGVIILGVMSFSFFILSLTLLKGKQHNSAVLSGTRNPVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME87</accession>
    <name>W5ME87_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01010958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME87"/>
    <dbReference type="FunCoup" id="W5ME87">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006696"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006704.1">
        <property type="protein sequence ID" value="ENSLOCP00000006696.1"/>
        <property type="gene ID" value="ENSLOCG00000005546.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156705"/>
    <dbReference type="HOGENOM" id="CLU_013615_1_3_1"/>
    <dbReference type="InParanoid" id="W5ME87"/>
    <dbReference type="OMA" id="RWAWASH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005546">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF3">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="116"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="414" mass="44464" checksum="ADDAEE144F6FDFC2" modified="2014-04-16" version="1">MAAVGDAQEAAGGGAESPGRRRRTSLLDGGEDFEMLNDEEEEDDDDDGDLPPLEDTAAGKKAAVRKEGEDGTGSEQSPAAEDSSEGEEWLDVLGNGQLRKKVLAAGRGPDSRPRKGQTVRVRLKASLQDGSLVQEEPSLDFTLGDGDVIQALDLAVQLMELEEKALIYSDAKYAYGTLGNESDPAAPPAAALSLEVQLLEASDAPDLELLPPREKIALADRKRARGNACYQRGDYVFAVNSYGIALQITESGSGVGITAQEEEELLDVKVKCLNNMAAAQLKLEHHEAALRSCVSVLAHQPDNIKALFRKGKVLALQGEYAEAIRILKRALKLEPTNKEYHSGISKPIAQNRLSPGTRVTLHKLFLHYLCEGGSEVPCVNKLGPRYCINWKWLFGATAVAIGGVALSVVIAARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEF5</accession>
    <name>W5MEF5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2-oxoglutarate and iron-dependent oxygenase domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AHAT01005137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEF5"/>
    <dbReference type="FunCoup" id="W5MEF5">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006772.1">
        <property type="protein sequence ID" value="ENSLOCP00000006764.1"/>
        <property type="gene ID" value="ENSLOCG00000005604.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1971">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153974"/>
    <dbReference type="HOGENOM" id="CLU_045835_1_0_1"/>
    <dbReference type="InParanoid" id="W5MEF5"/>
    <dbReference type="OMA" id="CQAFVDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005604">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24014">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24014:SF4">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25238">
        <property type="entry name" value="OGFOD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P4Hc"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="217"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="355" mass="40503" checksum="353BA5E6F2516C19" modified="2014-04-16" version="1">MDSTKGVVGQKFYSCACFCTDNIYLGEYKLHVKFISELQFRKDYQAVLRNHGCTTNQHFEDILKKIEAEIQRRRQLGVKSAERTAFISEFYKPLHRHVYVLQESYLAPEFLQIVNYSRSKHATVEGLHSFIQPLAAEAERVYRLPVFTKQFCTDLIEELENFEQSDSPKGRPNTMNNYGILLNELGFDEGLITPLRVDYLRPITALLYPDCGGDCLDSHKAFVVKYALGEDLDLSYHYDNAEVTLNVSLGQEFTEGNLYFGNMKEVPIHESECVEVEHRVMEGLLHRGQQLHGALPITAGQRWNLIVWMRSSEERNKLCPMCNKQPELVEGEGSADGFTKVPPSQNSTSTPCALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGE3</accession>
    <name>W5MGE3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB (POZ) domain containing 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01010825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGE3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007452"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007460.1">
        <property type="protein sequence ID" value="ENSLOCP00000007452.1"/>
        <property type="gene ID" value="ENSLOCG00000006170.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159179"/>
    <dbReference type="HOGENOM" id="CLU_015899_2_0_1"/>
    <dbReference type="InParanoid" id="W5MGE3"/>
    <dbReference type="OMA" id="KHCVDYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006170">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000004">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000004">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000078">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF3">
        <property type="entry name" value="BTB (POZ) DOMAIN-CONTAINING 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="34"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="423" mass="46578" checksum="C04E6E171C171449" modified="2014-04-16" version="1">MSETGPDPTRPDPLSGVARVSAGHVRIPLCVCGRFVLAVGSAVFDAMFNGGMATTSTEIELPDVEPAAFLALLKFLYSDEVQIGPETVMTTLYTAKKYAVPALETHCVEFLKKNLRADNAFMLLMQARLFDEPQLASLCLENIDKNTADAIAAEGFTDIDLDTLVAVLERDTLGVREVRLFGAVVRWAEAEAQRQQLQPTPENKRRVLGKSLSLIRFPLMTIEEFAAGPAQSGILMDREVVSLFLHFTVNPKPRVDFIDRPRCCLRGKECSITRFGQVESRWGYSGTSDRIRFSVNRRIFVVGFGLYGSIHGPTDYQVNIQIIHTDSNTVLGQNDTGFSCDGSANTFRVMFKEPVEILPNVNYTACATLKGPDSHYGTKGLRKVTHESPSTGTKTCFTFCYAAGNNNGTSVEDGQLPEVIFYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGE9</accession>
    <name>W5MGE9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tektin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGE9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007458"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007466.1">
        <property type="protein sequence ID" value="ENSLOCP00000007458.1"/>
        <property type="gene ID" value="ENSLOCG00000006174.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182894"/>
    <dbReference type="HOGENOM" id="CLU_033588_2_1_1"/>
    <dbReference type="InParanoid" id="W5MGE9"/>
    <dbReference type="OMA" id="HIEDRCH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006174">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF11">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="486" mass="55541" checksum="6EF9324E7AFCA2AA" modified="2014-04-16" version="1">MDFFGSTQSCAYFRPAPPVGRVLPNLSTMKDSYKCFARPQPVYHHLNLDWKPKTRYKISFGEQNDADGPSGDDTKMPPLAPSNAAPTTPEEWRRANTAHLRQSEASRRQAEKMRAETSRLIQVQDQLTRKAQSETSRRIGERMTDIAFWTNELSYEVEQLVRDSEMLKGVKTRLEKALSVTEGPKQMAKECLYHRQKKLGNELITDTVEKELVQEVSVIASCQEQIQRLIDKTMKQLETNRKVQHVMEKDIDDKHAAYHIEDRCHKLMDFTSGIAFFMDVDRMDVSFCSASVPESWAKFSENSILLSQSERAATAKVRDEVERVVEVTSREMWSQYHRVNIAFASCISETAEAKDKLQAHLAKTLQEVFQTERTISALEEAIRNKEAPLKVAQTRLKERSKRPNIELCKDPPHSKLLTEIHSINGTVASLKLHLAEARDSLQQLVNSKATLEQELFAKAHSLFIDREKCMGIRKIFPSSPRLAGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGP9</accession>
    <name>W5MGP9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNA binding motif protein 24b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635821.1">
        <property type="nucleotide sequence ID" value="XM_006635758.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213020.1">
        <property type="nucleotide sequence ID" value="XM_015357534.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213021.1">
        <property type="nucleotide sequence ID" value="XM_015357535.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGP9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007566.1">
        <property type="protein sequence ID" value="ENSLOCP00000007558.1"/>
        <property type="gene ID" value="ENSLOCG00000006244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695671"/>
    <dbReference type="KEGG" id="loc:102695671"/>
    <dbReference type="CTD" id="405801"/>
    <dbReference type="GeneTree" id="ENSGT00940000156245"/>
    <dbReference type="HOGENOM" id="CLU_065652_0_1_1"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006244">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000077">
        <property type="entry name" value="RNA-binding motif protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="230" mass="24469" checksum="ADF358D4045269FB" modified="2014-04-16" version="1">MHTTQKDTTYTKIFVGGLPYHTTDSSLRKYFEVFGEIEEAVVITDRQTGKSRGYGFVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRVMQPGFTFGVQQFPPAFIQRPYGIPAHYVYPQAFVQPSVVIPHVQPAAATAAATSPYIDYTGAAYAQYSAAATAAAAAAYEQYPYAASPAAAGYVTTAGYGYAVQQPLAAAAPGSAAAAAAAFGQYQPQQLQTDRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGX5</accession>
    <name>W5MGX5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRELI domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024330">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRELI_MSF1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="171" mass="19063" checksum="91415C1DB9354500" modified="2014-04-16" version="1">MKIWTSEHVFNHPWETVTKAAMQKYPNPMNPSVVGVDVLDRHVDPHGRLHSNRLLSTEWGLPSIVKSLIGATRTCTYIQEHSVVDPGEQTFELKSTNITLTNMVSVDERLIYRPHPQDPEKTMLTQEALITVKGVSLSSYLEGVMANTISSNANKRSPPPTAGVISLEEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH93</accession>
    <name>W5MH93_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Family with sequence similarity 210 member B</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
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        <location>
            <begin position="34"/>
            <end position="57"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="2">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="130" mass="13964" checksum="A9F3CB8C3D54B26F" modified="2014-04-16" version="1">LRLSQPPGGVPSPEQSQDGRISKTQQLRKVFKEYGAVGVTFHVGISLVSLGMFYLAISSGIDMAAVLFKLGFSEAVVQSKMAAGTSTFVLAYAVHKLFAPVRISVTLVSVPFVVRYFRKTGLFKPPASSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHN1</accession>
    <name>W5MHN1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000007890"/>
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        <property type="protein sequence ID" value="ENSLOCP00000007890.1"/>
        <property type="gene ID" value="ENSLOCG00000006530.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006530">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042344">
        <property type="entry name" value="ZCCHC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16195">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16195:SF16">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="145" mass="16405" checksum="55406659E27D4328" modified="2014-04-16" version="1">MHKISLLFLPVIFMYFVVFTVNIERIELKTQAHRKSEKLAEYLFEVLWSDSTVSTVLKAAPDLTEFLSKLPQLFPDEDFEKLIPQLPGLDSAPDPDPRCLVSLPSHVLKNDQVKHFFSAPSVAQCPQSTNFAYVSQHKASSTAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI95</accession>
    <name>W5MI95_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="26">Receptor tyrosine kinase like orphan receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI95"/>
    <dbReference type="FunCoup" id="W5MI95">
        <property type="interactions" value="800"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008104"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008114.1">
        <property type="protein sequence ID" value="ENSLOCP00000008104.1"/>
        <property type="gene ID" value="ENSLOCG00000006711.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153947"/>
    <dbReference type="InParanoid" id="W5MI95"/>
    <dbReference type="OMA" id="IQNDECP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006711">
        <property type="expression patterns" value="Expressed in heart and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05090">
        <property type="entry name" value="PTKc_Ror1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000002">
        <property type="entry name" value="Inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000116">
        <property type="entry name" value="inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000003">
        <property type="entry name" value="Inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000242">
        <property type="entry name" value="Inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000139">
        <property type="entry name" value="inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
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    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016247">
        <property type="entry name" value="Tyr_kinase_rcpt_ROR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF134">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000624">
        <property type="entry name" value="TyrPK_TMrec_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4 19" id="KW-0420">Kringle</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="25">
        <location>
            <begin position="42"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="23">
        <location>
            <begin position="169"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="24">
        <location>
            <begin position="316"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="474"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="754"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="833"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="755"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="854"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="616"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="480"/>
            <end position="488"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000624-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000624-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="938" mass="104398" checksum="32D84AD1DE5C6F79" modified="2014-04-16" version="1">VFGFGERKQKTDYNKGLLAKVENLILQCRGGCHDTSALSCDPQLTATPSWNISSDADKDYFLKLEAPMNNITTSQGQTAELHCKVSGNPTPVIRWLKNDAPVVQEPRRVSFRATSYGSRLRIRNLDTTDTGYFQCVATNVKGTVSTTGVLFVKFVPDGTVSPSIQDEYDEEGFCQPYRGIACARFIGNRSIFVDSLQMQGEIENQITAAFTMIGTSSHLSDRCSQFAIPSLCHFAFPFCDRTSGVDKPRDLCKDECEILENDLCKTEYIIARSNPVILKRLKLPNCDDLPASDSREAANCMRIGIPMAEPINKNHKCYNSSGVEYRGTVSVTKSGLQCQPWNSQYPHSHTFLAVRYPELNGGHSYCRNPGSQHEGPWCFTLDDSVRMELCEIPVCDFKESSKMGILYILVPSVAIPLAIALLFFFICVCRNNQKTSRPPAQRQPKPVRGQNVEMSMLNTYKPKSKAKELPLSAVRFMEELGECTFGKIYKGHLYLPGMDHAQLVAIKTLKDYSNPQLWGEFQQEASLMSELHHHNMVCLLGVVTQEQPICMLFEFLNQGDLHEFLIMRSPHSDVGCSSDEDGTVRSSLDHGDFLHVAIQIAAGMEYLASHFFVHKDLAARNILVGEQLQVKISDLGLSREIYASDYYRVQPKSLLPIRWMPPEAIVYGKFSTDSDIWSFGVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCPEDCPPRMYGLMTECWQENPSRRPRFKDIHTRMRTWEGISSHASSTTPSGGNATTQTTSLSASPVSNLSNPRYANYIHIPQGIPQGQIAGFMTAQMPQNQRFIPVNGYPIPTGYAAFPAAHFQPQGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSVPSTGSNHDANTPLLAHCMTLSNLPSGAAVPVFGHMNQKPMPIDPTQATLLSDSNRHMYSESVITADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIS1</accession>
    <name>W5MIS1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Early growth response 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIS1"/>
    <dbReference type="FunCoup" id="W5MIS1">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008280"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008290.1">
        <property type="protein sequence ID" value="ENSLOCP00000008280.1"/>
        <property type="gene ID" value="ENSLOCG00000006850.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688444"/>
    <dbReference type="KEGG" id="loc:102688444"/>
    <dbReference type="CTD" id="30190"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158394"/>
    <dbReference type="HOGENOM" id="CLU_043235_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIS1"/>
    <dbReference type="OMA" id="QCQRELH"/>
    <dbReference type="OrthoDB" id="8197458at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006850">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021575">
        <property type="term" value="P:hindbrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048932">
        <property type="term" value="P:myelination of posterior lateral line nerve axons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021654">
        <property type="term" value="P:rhombomere boundary formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000092">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF54">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE EGR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="276"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="435" mass="47516" checksum="F59321A86E57B628" modified="2014-04-16" version="1">MMTAKTLEKTPVSLSGFVHTLPENIYSVDEISTTLPASVAIFPNGDLGGHFDHISGVSGDGMINGDMSTEKRSLDLAYSSSFTQPTAPRNQTFTYMGKFSIDSQYPGSNWNPESVINIVSAGILGMSQPSSSSSSPASSVSPNHFSSTLSCSMAQNQPEMDHLYSPPPPYSGCGEVYQDPSAFLSTSTCPISYPPPSYSSPKPASDSALFPIIPDYSSLFQPPCQRDIQSVPDRKPFPCPLESIRVPPPLTPLNTIRNFTLGGPAADGPRLPAAYSPQNLPLRPILRPRKYPNRPSKTPVHERPYPCPAEGCDRRFSRSDELTRHIRIHTGHKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDFCGRKFARSDERKRHTKIHLRQKERKSASVAPTNTGAQNSERSVSISTSSGGVCQTGTNQLSACPSRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK38</accession>
    <name>W5MK38_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 G1</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-protein ligase G1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01005193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK38"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008747"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008757.1">
        <property type="protein sequence ID" value="ENSLOCP00000008747.1"/>
        <property type="gene ID" value="ENSLOCG00000007230.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165298"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_1_1"/>
    <dbReference type="InParanoid" id="W5MK38"/>
    <dbReference type="OMA" id="MLCLNSQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007230">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000018">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="6"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="171" mass="19600" checksum="E95FAFB942936440" modified="2014-04-16" version="1">HTMTEQSALLLRKQLAELNKNPVEGFSAGLIEDDDIYKWEVVIIGPQDTLFEGGFFKAYLTFPYDYPLRPPKMKFITEIWHPNVAKNGDVCISILHEPGEDKFGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDPNGEFKRKVARCVRKSQEMAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKP6</accession>
    <name>W5MKP6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fraser extracellular matrix complex subunit 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRAS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000007363.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in larva and 11 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:basement membrane organization"/>
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        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
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        <property type="term" value="P:fin morphogenesis"/>
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        <property type="entry name" value="Extracellular matrix protein FRAS1 isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
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        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="5"/>
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    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="4"/>
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    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Calx_beta"/>
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        <property type="entry name" value="CSPG_rpt"/>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
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        <property type="entry name" value="Furin_repeat"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="VWF_dom"/>
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    <dbReference type="PANTHER" id="PTHR45739:SF1">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="6"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="8"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="VWC_out"/>
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        <property type="entry name" value="FnI-like domain"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868340" evidence="14">
        <location>
            <begin position="26"/>
            <end position="4004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3898"/>
            <end position="3919"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="92"/>
            <end position="152"/>
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    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="156"/>
            <end position="216"/>
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        <location>
            <begin position="218"/>
            <end position="278"/>
        </location>
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    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="282"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="371"/>
            <end position="416"/>
        </location>
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            <begin position="1099"/>
            <end position="1194"/>
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            <end position="1303"/>
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        </source>
    </evidence>
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        </source>
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    <evidence type="ECO:0000259" key="15">
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        </source>
    </evidence>
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    </evidence>
    <sequence length="4004" mass="447779" checksum="9F9B8AD2E7912EFD" modified="2014-04-16" version="1">MVCRKLWVCVAVLTGFVTIPRGIDGECQYHGSLFANNTVWKPDSCQDCSCLNDIVICKMTRCQNPECDFLKGEQLRVPPNKCCPECDLQTQGFCQYEGQIYGHDSQWSISKCTVCLCSHGKVECSPKSCPLLHCGREETQLISSGDCCPKCVRNGVSCSFEGQEYKDGMEWQPRPCSKCSCRNGETICYIIECQALICKPKENFVIYPGKCCPQCVPNSCLVSGKEYKHGEQWKKNSCTACICDQGEVRCHTQTCPKLSCERGQNKVKKVGLCCEECVSSKGNCLYDNSIHYHGEMWNSTGCEFCMCDQGQVLCQKAECARVDCTWGEQLVHFPGRCCPECTSMSSFCVYKEHTKKLPNPGNAEEWSKNLEKWQDDPCKECECRDSQVICYQRSCHTCPVGTLAVTVEGECCPECLAVECHSDCLTCFQSPDHCNSCRNESLLLQGGHCVRTCSRGFFKNGRVCSACKETCATCDSEFECTLCAESLLIRNGKCVRSCGKGFYQEHTHCAACHDSCSSCQGPGEQACLSCADPSLFLRSGRCVPSCGLGFYVKQGTCYACDRSCLMCYPDSSKCLRCPSGRALHQGKCIPLCPKGYYKDKANRCSECHSSCADCSGPSASQCSVCPAGLVLHQGTCLDMCGEGFYSHLGVCKICHQSCRSCVGPKHSDCVQCLKPEEVLHSDQDHNGLLHGVCVSECKAQFYLETDMICRECHPTCMKCIGNSAHNCTACAYSNVLHEGKCLHKCPEGLYSQNNICSTCHPSCKECTGPSEAECTVCHPHTTLLDGYCRNSCPKGQYLNVVGYCTDCHSECQHCTADMRDNGSICLQCKNPKYFMLENKCIPYCLPGYYTYRGSCEKCHPSCKTCSAEDPLSCTSCHINLVLTHTGLCSTQCFPGHYVNKKGICQACDGQCLTCDVPGSCTSCRDHAKVLLFGECHESCAQQYYLDFTTRTCRECDWSCNACRGPLSTDCLQCMDGYVLQDGSCIQQCSTGFYRDGEKCQRCEVHCEECEELYQCEKCKNPYVVLQGQCVHECGKKYFLDRFLHKCIACSTDCLECDSAHQCRVCNKNTYLKDGVCTPDCGYGFNINRKNRTCKENKHAPVLYVNGSLIVKIGGTKQLDFSFMSVRDPDTSIENLIFQLVQPPNNGRLIKKINGKEFKLNREDTFTFTDLIENTVRFIHAKDKSKNGQLFLRAGDQQLFSQTETINIQAISLQVPFVLTNEVLLVNRGETVTITKSVLNIQDEDNLQDVLVMVLEPPQHGHLVHAHGDMPVQQFKPEELEQKQLKYAHDGSDGQQDTVILQMNDGHNFQNIFFHIMVAQKKGKSPHMLSIPTAWVHEGRMFQITSKYLKAKYHDLSDSEVHYTILPSKENLRYGEVVLLVPMPADGPADGWQHLPDGRVATTTTSFTQQDINEGIVWYRHFGSGSQEDNLQLQISRGGSTQALNKTQTLRIRVMPHSSQFPQLDPGSALQITAFEDHVTVLGPSALSFVDTGTPSEKFIYNITKPLAQGEGTIEHQDRPYIPVQFFTQADINQGKILYRPPMAPSHLREFSLYSFSGLPESVNFHFTVSDGEHTSPEMDLAIYLLSHHHQPPAFQIKDPFLEVSLGGKASIDQQLAVSDADTDPDDLVFELLKAPLHGTLVKKDFGSQTEMVNGEMFTFNDITRNALLYLHDGMNTEEDLMMFSVTDGISTVTVEVRVIVSGAASDGPQLASSSLLSMEVSEKSTTIIRRSHLAYVDNNSPDDKIRIQLISLPMYGVLTKTKSATVHEELLKYSSFTMEDINLERIRYSTSFETGSQPITDIFHFTVYDGDNNHLDNQMCTITITSVQNKPPVVSVTSGIMVEAGGRIQLSSNNILATDTDTQKKDLLVWLINHPKYGLIEKTVIGEISGSPEIINSDVPFSVEDILSNRMFYIQSIHKSDEHFQDSFSFYISDGYSQTEAFTININIQGKDKPPIISKNNINLEVASGVVITNSSLTVLDPDTPDNELIFTVTKMPGYGKLRKRQFYSQPLENGRILNQGSAFTYQDVLDELIVYTPDTVSVRTDELHFTLTDGVYTENGRLEFTMDGQKSEGPRLAINRGLQLTAGSVAKITDQNVKAVDIDSDELKLKYIMTTDPAVGWLQQSKNGNIAQISIKGPMKSFTQDDINKGFVEYRHEKGKAGGSFAFKFDLSDSEGNKLIDQSFYISVLEDRLPPSVIVNKGLVLDENSAKKITTFQLSATDQDSEPGELVYRITKQPLLGYLEHVATPGTQISTFTQADLASHSIQYVHTSDEEKHSDDFSFIVSDGANEITQTFYITIRPVDDSLPIVQVPGMRVQEGMRKTITEFELKTTDADTEEDSIIFTIVQAPRHGTIERTNNGQHFRQTTTFTMDDIYQNRISYNHDGSNSLKDRFTFTVSDGTNLFFVVKEREKEIVTAAPQKFKIDILPVDDGTPRIVTNLGLQWLEYMENKATNLITRKELLTMDPDTEDRQLVYEITAEPKHGYLETKLKPGTPITIFTQADINLGLIRYVLNEENILETMDTFKFLVKDSKPNVVSDNVFHIQWSLISFEYTSYNVSEKDGTVSVTVRRTGNLNRYAIVLCRTEQGTATSTSSTSSKPGQQDYVEYAGQVQFDEREDTKVCTIVINDDHIFENIESFNVELSMPVYALLGKVTRAKVNIIDTEDEPTLQFDKKTYHVNESAGFFFAPIERKGDSSSTVSALCYTVSKSAQGSSLYSLESGSDFKSRGMSSENRVILGPGVSMSTCDVKLIDDSEYELEEEFEIILADASDNARIGDVTVAKVVIDGPNDVSTVFLGNASFTLSEDKGVIEIPVIRQGSDLSSVTSVWCATRPSDPPSATPGVDYIPSSKKVEFRPGKTEEICSLTILDDNQNPTIEGTEYFVVFLSSPQRAVLTEPYEALVAINDAFQDVPSMQFEKDVYSVKEKNGVLHIPIIRTGDVTFESSVRCYTRTLSAKVMNDFEERKNTDDSRITFFKGEKVKNCTIWLNDDSVFEPEEAFQVHLGNPMGNHWSGSRVGKNDIATITITNDEDAPTIEFEEPSYQVQEPSGPDGIAEVNIKVIRKGDQNRTSKVRCSTRDGSAQSGMDYNPKSRLLRFTPGVDHILFKVEILSNEDQEWHESFTVVLGPDDPVEAVLGEITMATVTILDQEAAGSLILPAPPIVVSLSVYDCVQEVSKESSKNSPSPGYPLICVTPCDPHYPKFYLMKDLCDEAGINQSSVHFNWEVAAPTDSSGARSPFETVTDNTPYTSVNHKVLDSMYFSRRFHVRCVAQAVDKAGHLGTPLRSNIVTIGTENSICHTPVASGTARGFQAQSFIASLKYLDVKHKEHPNRIHISVQIPHQDGMLPLVSTMPLHNLHFLLSESIYRHQHICSNLVTLKDLQGISDTGFLDNLSYDSISLGPGYDRPYQFDPTVREPKTIQLYKHLNLKSCIWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVQVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIYIREDGRLVIEFKTHAKFRGQFVLEHHTLPGQRSRLMAPDHLGGIDFELQLLWSAQSFDSPYQLWRATSSYSRKDYSGEYTVFLMPCTVQPTQPWTDHGDKPLSCTAHAPEKFLVPIAFQQTNRPVPVVYSLNTEFQLCNNEKIFMIDPAVAEMSLAEMDYKGAFSMGQTLYGRVLWNPEQNLNVAYKLKLEKVYLCTGRDGYVPFFDPTGTIYNEGPQYGCIQPNKHLKHRFLLLDRKQPEVCDKYFHDVPFEAHFATDIPELQSMTAMSGVDGFTMKVDALYKVEAGHQWYLQVIYIIGPEASSGPRIQRSLTYQLKRSQRDLVDNTGRLTLDESLIYDNEGDQVKNGTNMKTLKLEMQAAAFTAQTSNFIGGAIAAVIFLSLIVFTFCFLSKKSYRKGKKKRHATNLAEKYPLNTKVEDCLRNVDHLEKNLIAKHCTVKNNILNKNQEACKVKEVKLKQVNLVKCHNNLHDGTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLI5</accession>
    <name>W5MLI5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009244"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009255.1">
        <property type="protein sequence ID" value="ENSLOCP00000009244.1"/>
        <property type="gene ID" value="ENSLOCG00000007613.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159313"/>
    <dbReference type="HOGENOM" id="CLU_000624_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLI5"/>
    <dbReference type="OMA" id="TFYHLGC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007613">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000022">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037808">
        <property type="entry name" value="C2_Dock-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF65">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="387"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1499"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="713"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="766"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="715"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1961" mass="223009" checksum="36AF4B4777B2B2CD" modified="2014-04-16" version="1">QEDLKRRSVSLDDTPRGSWASSIFDLKNSAPDCLLPSLLERNAPEEIDNRNAEKRREGRHPDLLGLFPAPDEDEAVERCTIPEVPKEHFGQRIMVKCLSLKFEIEIEPIFGVLALYDVKEKKKISENFYFDLNSDQMKSMLRPHTPHVAISTLTRSAIFSITYPSPDIFLVIKLEKVLQQGDIGECCEPYMVMKESDSAKNYEKYNCLIKSDSKTFCCSKVASPFRFGISNQNCIKTGLNTKRFTKDIFVTLGARSGFKCTCRHCSNCPKTVLPLFHQGDHLMQLHFSQLPSTQLTQQFEEEGDRLSDEDLYKFLADMRRPSSLLRRLRPVTAQLKIDISPAPDSPHYCLSPELLHVKPYPDLRVRPTKEVLEFPARYIYIPYTTYRNLLYVYPQTLNFSSRQGSVRNIAVKVQFMAGEDPSQALPVIFGKSSCGEFYKEAYTPVIYHDKTPEFYEEIKLKIPANLTDNHHFLFTFYHISCQQKQNTPLETPVGYTWIPLMQHGRLRTGSFSLPVSVEKPPPSYSVLTPDVQLPGMKWVDNHKGVFTVEVTAASSVHTQDPHLDKFFTLVHVLEEYSFPFRLKDVIITEGNVEAELKSSIGQLRSAVLETCIRFLHHLLNKLILLIVRPPVIAGQIVNLGRAAFEAMALLVNQIHKNLEGNQDQHGRNNLLSSYIHYCFHLPTLETGPPSSYTGSAPYGMPIQYATLSRATGRPSSLNLSRSKSISNSNPDLASTPTSPDDEVQKIIGNKGIDRSHSWVNSAYAPGGPKAVLRRNHPNSSSDLKQASERCPNRMSAFLEGTSHAAPTPARQTARKLLHEELALQWVVSTSTVRDAALHQAWFFFQLMVKSMAHHLYLSDRLDTPRKQRFPDRFVDDISALVCAISADIASRYHKDVELIERLNSSLAFFLNDLLSLMDRGFVFNLIRSYCKQIGNKLHTTQNPNALVALRMDFVRIVCSHEHYVTLNLPCSTLSPPASPSPSISSATSQSSAFSSHLQDLRVASMFELSVPFRQQHFLSGLLLTELALILEPDGEGVFFLHKKAISAVHNMLCSHDSDPRFTDPQVRTHLAQLYLPLIGIVIETLPQLYDFTENSPPRVRHASALADDIDSDNGSSINQSVAMAIAGSPLPHYKANPFQLPIVAGRQSSILSAESSRNLLICFLWVLKNADSEILERWFSDLSVLQINRLLDLLHLCLSCFEYKGKKAFERINSLTFKKSQDMKARLEEAILGSIGARQEMVRRSRGNNRAAERSPYGGQENVRWRKNITHWRQNTDRVDKTKAEMEHESIVDGNLATEASLVVLDTLEIIVKTVLLSETKESILGGVLRVLLHSMACNQSALFLQHCFATQRALVFKFPEMLFEEDTELCADLCLRLLRHCSSSVGTVRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGMSQNFNEEHLRRSLKTILTYAEEDLELRDTPFPEQVQDLVFNLHMILTDTVKMKEHQEDPEMLIDLMYRIAKGYQNSPDLRLTWLQNMAGKHSERGNHAEAAHCLVHSAALVSEYLNMLEDCRYLPIGCVSFQNISSNVLEESAVSDDILSPEEEGICAGKYFTETGLIGLLEQAAASFNMAAMYEAINEVYKILIPIHEANRDFKKLATIHGKLQDAFNKVNNQQTMKRMFGTYFRVGFYGCRFGDLDEQEFVYKEPSITKLAEISHRLEEFYAERFGDDIVEIVKDSNPVDKTKLDPNKAYLQITYVEPFFDTYELKERITYFDKNYNLRTFMYCTPFTLDGRAHGDLHEQYKRKTILTTSHAFPYIKTRINVTHREEIILIPIEVAIEDMQKKTQELAFATHQDPADAKMLQMVLQGSVGTTVNQGPLEVAQVFLADIPDDPKLFRHHNKLRLCFKDFTKRCEDALRKNKALIGPDQKEYHRELERNYNRLKESLHPLLNRKIPQLYKPLVMHHASQRNSFSRSSLRKVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM00</accession>
    <name>W5MM00_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrix metalloproteinase-14</fullName>
            <ecNumber evidence="6">3.4.24.80</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.</text>
            <dbReference type="EC" id="3.4.24.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="26">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="26">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.80" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01024190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639739.2">
        <property type="nucleotide sequence ID" value="XM_006639676.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM00"/>
    <dbReference type="FunCoup" id="W5MM00">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009409"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009420.1">
        <property type="protein sequence ID" value="ENSLOCP00000009409.1"/>
        <property type="gene ID" value="ENSLOCG00000007747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698043"/>
    <dbReference type="KEGG" id="loc:102698043"/>
    <dbReference type="CTD" id="10893"/>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158315"/>
    <dbReference type="HOGENOM" id="CLU_015489_8_1_1"/>
    <dbReference type="InParanoid" id="W5MM00"/>
    <dbReference type="OMA" id="AMTQHYY"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007747">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.101.10:FF:000002">
        <property type="entry name" value="Matrix metalloproteinase-14 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF138">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 26" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 31" id="KW-0732">Signal</keyword>
    <keyword evidence="12 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="22" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016797603" description="Matrix metalloproteinase-14" evidence="31">
        <location>
            <begin position="35"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="32">
        <location>
            <begin position="119"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="338"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="387"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="434"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="483"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="296"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" evidence="24">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="26">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="189"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="191"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="196"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="197"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
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        <location>
            <position position="204"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="213"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
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        <ligand>
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        <ligand>
            <name>Zn(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
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        <location>
            <position position="222"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <location>
            <position position="222"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="242"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="246"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
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        </ligand>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
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        </ligand>
    </feature>
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        <location>
            <position position="260"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        </ligand>
    </feature>
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        <location>
            <position position="391"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
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        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001718"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00012342"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00020199"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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            <dbReference type="SMART" id="SM00235"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="606" mass="70025" checksum="397C427E5313ACD3" modified="2014-04-16" version="1">MTGMGVKRRKAGLPGFCWKTCYFHILFWVVSVCGEEKTFIVETWLKNYGYLLPHDIRTSDLRSEKAMQSAVAAMQRFYGIPVTGVLDQTTIEWMRKPRCGVPDHPRISRRRRNKRYALTGQKWRQKHITYSIHNFTPKVGEKDTQKAIRQAFDVWETVTPLTFEEIPYSEIKNERKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNANHDGNDLFLVAVHELGHALGLEHSNDPSAIMAPFYQYMETHNFKLPLDDLQGIQKIYGIPTAMLEPTRPLPTLPARRTHSTSERKHDRQSRPPRPPSGDKPASPGTAKPNICDGNFNTVAFFRREMFVFKDRWFWRLRNNKVQEGYPMQIDQFWKGLPPRIDAAYERSDGKFVFFKGDKYWVFKEVTAEPGYPHSLVDLGNCLPREGIDTALRWEPVGKTYFFKGDQYWRYNEEKRTADPGYPKPITVWKGIPESPQGAFVSKEGFYTYFYKGKEYWKFDNQKLNVEPGYPKSILKDWMGCHQSDVEKSKERHLPHDDVDIMVTINDVPSTVNAIAVVIPCILSFCILVLVYTIFQFKNKGVQQNVTYYKRPVQEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM18</accession>
    <name>W5MM18_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="6">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EIF6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <text evidence="6">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639830.2">
        <property type="nucleotide sequence ID" value="XM_006639767.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM18"/>
    <dbReference type="FunCoup" id="W5MM18">
        <property type="interactions" value="1480"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <sequence length="245" mass="26668" checksum="62E3FC4D96C74525" modified="2014-04-16" version="1">MAVRASFENNNEIGCFAKLTNRYCLVAIGGSENFYSVFEGELSETIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNCLPDEVQIQRVEERLSALGNVIACNDYVALVHPDLDRETEEILADCLKVEVFRQTVAEQVLVGSYCTFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVIESVFRLSDAQPSAIATSMRDSLIDSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQI7</accession>
    <name>W5MQI7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">3-oxo-5-alpha-steroid 4-dehydrogenase</fullName>
            <ecNumber evidence="18">1.3.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14 18">Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5alpha-pregnane-3,20-dione + NADP(+) = progesterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17026"/>
            <dbReference type="ChEBI" id="CHEBI:28952"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:21954"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>androst-4-ene-3,17-dione + NADPH + H(+) = 5alpha-androstan-3,17-dione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:50816"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:50817"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01020205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQI7"/>
    <dbReference type="FunCoup" id="W5MQI7">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010646"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010661.1">
        <property type="protein sequence ID" value="ENSLOCP00000010646.1"/>
        <property type="gene ID" value="ENSLOCG00000008745.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684018"/>
    <dbReference type="KEGG" id="loc:102684018"/>
    <dbReference type="CTD" id="6715"/>
    <dbReference type="eggNOG" id="KOG1638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
    <dbReference type="HOGENOM" id="CLU_065395_1_1_1"/>
    <dbReference type="InParanoid" id="W5MQI7"/>
    <dbReference type="OMA" id="PHYALEW"/>
    <dbReference type="OrthoDB" id="5788137at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008745">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006702">
        <property type="term" value="P:androgen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000002">
        <property type="entry name" value="Steroid 5 alpha-reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016636">
        <property type="entry name" value="3-oxo-5-alpha-steroid_4-DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF57">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015596">
        <property type="entry name" value="5_alpha-SR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0726">Sexual differentiation</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="19">
        <location>
            <begin position="117"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR015596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="30212" checksum="5879B7226245C398" modified="2014-04-16" version="1">MCKALSNLFGSEAEEYYLLECMSYLMIFMAAVTFLVLLFENVPYGRYATSKYGFPVNARFAWFVQELPALVVPVLLLLTASSARASYLPNQLLLAMYLLHYFQRSLIYPFLIRGGKPTPFLSFVMAFLFCVYNGYLQGRYLSHFAKYPADWIMHPCFIIGFLMWLLGVLINVHSDHILRNLRKPEETGYKIPQGGLFEYVSGANFLGEIVEWAGFAVATCSLQSAAFAIFTSVVLSSRAVSHHGWYLQKFEDYPKSRKALVPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQN2</accession>
    <name>W5MQN2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="2">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01016274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214683.1">
        <property type="nucleotide sequence ID" value="XM_015359197.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQN2"/>
    <dbReference type="FunCoup" id="W5MQN2">
        <property type="interactions" value="996"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010691"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010706.1">
        <property type="protein sequence ID" value="ENSLOCP00000010691.1"/>
        <property type="gene ID" value="ENSLOCG00000008781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687849"/>
    <dbReference type="KEGG" id="loc:102687849"/>
    <dbReference type="CTD" id="64172"/>
    <dbReference type="eggNOG" id="KOG2707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153744"/>
    <dbReference type="HOGENOM" id="CLU_023208_1_2_1"/>
    <dbReference type="InParanoid" id="W5MQN2"/>
    <dbReference type="OMA" id="NAAMIGC"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008781">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24134">
        <property type="entry name" value="ASKHA_NBD_OSGEPL1_QRI7_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000106">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="73"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="429" mass="46763" checksum="7C2DE6455FD2E66B" modified="2014-04-16" version="1">MSLLSCFGARLCGLAFQRATVTEPHQWKLKEVLSWASYRTTCIRTVSHHRVVLGVETSCDDTGAAVMDETGSVLGESLYSQKEVHLKTGGIIPTVAQRLHREHITTVIQQALAMSGVTPNELSAVATTVKPGLALSLGVGLEYSLKFVQQYKKPFIPIHHMEAHALTVRMIHSVQFPFLVLLVSGGHCLLAVANGIDDFLLLGQSLDEAPGDTLDKVARRLSLIKHPECSTLSGGQAIEHLAKQGDRLKFYLKSPMGQHLDCNFSFAGLRNQVTQVIIKKEEGEGVQMGQMLSCANDIAAAVQHTVASHIAKRTHRAILFCKAKGLLPQNSPTLVVSGGVASNQYIKKTLKIVTDATDLTLLCPPSKFCTDNGVMIAWNGIERLREGKGILNHIEHICYEPKASFGTDISGQVKEAAIKIPRLKLKIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQT4</accession>
    <name>W5MQT4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GID complex subunit 4 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637289.1">
        <property type="nucleotide sequence ID" value="XM_006637226.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQT4"/>
    <dbReference type="FunCoup" id="W5MQT4">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010743"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010758.1">
        <property type="protein sequence ID" value="ENSLOCP00000010743.1"/>
        <property type="gene ID" value="ENSLOCG00000008829.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698969"/>
    <dbReference type="KEGG" id="loc:102698969"/>
    <dbReference type="CTD" id="79018"/>
    <dbReference type="eggNOG" id="KOG4635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044930"/>
    <dbReference type="HOGENOM" id="CLU_028759_2_0_1"/>
    <dbReference type="InParanoid" id="W5MQT4"/>
    <dbReference type="OMA" id="HWSKFQC"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008829">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="25104" checksum="42D61A9A542B08F6" modified="2014-04-16" version="1">MPVRTECCSTACAAFIASASLIPPPPINTQQPGVATSLLYSGSKFRGHQKSKGNSYDVEVVLQHVTMEDSYLCGYLKIKGLTEEYPTLTTFFAGEIISKKRPFLTRKWDADEDVDRKHWGKFQAFYQYAKTFNSDDFDYEELKNSDYVFMRWKEQFLVPDHTIKDISGASFAGFYYICFQKSTATIEGYYYHRSSEWYQSLNLTHVPEHSAPIYEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR00</accession>
    <name>W5MR00_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome complex component RRP41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Exosome component 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ribosomal RNA-processing protein 41</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC2, EXOSC7 and EXOSC9. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with DDX60. Interacts with DIS3; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR00"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010809"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010825.1">
        <property type="protein sequence ID" value="ENSLOCP00000010809.1"/>
        <property type="gene ID" value="ENSLOCG00000008878.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="HOGENOM" id="CLU_063514_0_0_1"/>
    <dbReference type="InParanoid" id="W5MR00"/>
    <dbReference type="OMA" id="RYNMAPF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008878">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000010">
        <property type="entry name" value="Exosome complex component RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="15">
        <location>
            <begin position="22"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="16">
        <location>
            <begin position="156"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="246" mass="26653" checksum="96C311F241132401" modified="2014-04-16" version="1">NMAGLELLSDQGYRLDGRKPSELRKVQARMGVFAQGDGSAYIEQGNTKALAVVYGPHEVRGSRGKALHDRAVINCQFSMATFSTAERKRRPHGDRKSSEVSQHLKQTFEAAILTQLYPRSQIDIYVKILQSDGGNYSASVNAATLAVIDAGIPLRDYVCACSAGFVEDTPLADLCHAEEAGGGTVLSLALLPQGGQIALLQMDARLHQDHLDTLMEAAMTACRSLSTVLDRVVREHLQEVTTLTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRL6</accession>
    <name>W5MRL6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Axonemal dynein light chain domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01032267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRL6"/>
    <dbReference type="FunCoup" id="W5MRL6">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011041.1">
        <property type="protein sequence ID" value="ENSLOCP00000011025.1"/>
        <property type="gene ID" value="ENSLOCG00000009045.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSV4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005554"/>
    <dbReference type="HOGENOM" id="CLU_006021_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRL6"/>
    <dbReference type="OMA" id="KKECYEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009045">
        <property type="expression patterns" value="Expressed in muscle tissue and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052845">
        <property type="entry name" value="Axonemal_dynein_LC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23052">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT CHAIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23052:SF1">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT CHAIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="876"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="925" mass="106330" checksum="3A713F3B7B705A38" modified="2014-04-16" version="1">NDFIPDEILLTLTSTVKPSSPTHVLGPPRITRTPKEFKGHGLRGADPVWRYPERRNKYKYFFDQPTSVTGAGRDISFLCDGIASKRAITPVPSPSDQRERYIHGSRTNELGLMESLIPEEYHIVKNKGVLGLECYEDKYTVLLEDSDKRLRVFPSLKPSGRLEAVQLMRVMDTMLEKAGISDDISEVTDLSQMHSLLELVKIEQNIYNVVFHELIRQITVECAERGKLLSKLRQRYILLLDRIPRQLKSLHTETVALRALDRRLTEEIVCFKTSIGQLHNELTLLRQHDLRVSKAAECAQEEMARVLQQAQKNADLVGEYHELYEMQRRRLESQVSHLTEERDLWSRVTYSLALKVIKANNLHFASKLQISEQTWSKTAEHFTILLMSKDTEDFGQILQLADQWKSLVGHFMQDLRQSENSVFEKVKMIEARIAKWQKLYEEKVKANEGSQKDLEQDLFQELKQWQEMLTYECERYGGDKLLSSQEALKTMVQLQEKWMDLALVLFRRHPGPDGETSHCQVMMMDLGRILEELHKQLGIRITGESGIHKIMLSLVGEIEFWSSRLSAVSGLSGGLPYSDWLKLQKAFSGWSKFVEETFQLIDCTHPESDQLKQKAYVRVDIEEAFHKQQEFLSMLSNIIEFENYRLKEECSAIHISLTQWILDLLLQMVPDCCDEKKLQPSSEKEAKNVVAIKRLEEDAMYLTQKLDHFSKYLTSSCQAIVEEDLQKHTAEDEPVDGLYELTKLQVEECSEWLETCQILLSEIKGCPLEQLAPSTQVVSFVTDLPPVQDSEESVVSIETDESLITGEADRHPPEVSESKSIEISKETSSLRFIGHDGNIQEKTLRELTVPLKEDDDVVDRPETPNAQRAFEALAAVDVLQRELLSAEERAQRAEKKALSTEEQLRSALEKIQNLEQQLKGRTNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRV7</accession>
    <name>W5MRV7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRV7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011132.1">
        <property type="protein sequence ID" value="ENSLOCP00000011116.1"/>
        <property type="gene ID" value="ENSLOCG00000009117.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166257"/>
    <dbReference type="HOGENOM" id="CLU_1975555_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRV7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009117">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="127" mass="14370" checksum="15B8F0E7399F8B37" modified="2014-04-16" version="1">MFVDKLYQTGTGKKEGWPNSLQTKEETAKFLTMIMFTCSAQHASVNNGQYDSYAWMPNGPTTMRQPPPKLKKDVTEEYIMNTLPDINVTLESMSVARFLSQTSPDTVSMQTHICTAWKIHGNLNSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS15</accession>
    <name>W5MS15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase SUPV3L1, mitochondrial</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Suppressor of var1 3-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
            <location evidence="3">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01003987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS15"/>
    <dbReference type="FunCoup" id="W5MS15">
        <property type="interactions" value="1331"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011174"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011190.1">
        <property type="protein sequence ID" value="ENSLOCP00000011174.1"/>
        <property type="gene ID" value="ENSLOCG00000009159.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687752"/>
    <dbReference type="KEGG" id="loc:102687752"/>
    <dbReference type="CTD" id="6832"/>
    <dbReference type="eggNOG" id="KOG0953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003100"/>
    <dbReference type="InParanoid" id="W5MS15"/>
    <dbReference type="OMA" id="QPANWYT"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009159">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.272.40:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041082">
        <property type="entry name" value="Suv3_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041453">
        <property type="entry name" value="Suv3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18147">
        <property type="entry name" value="Suv3_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18114">
        <property type="entry name" value="Suv3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="357"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="689"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="777"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="689"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00021960"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00031873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="808" mass="90444" checksum="E0718854A62E52CF" modified="2014-04-16" version="1">MSLKHSMCLLSRVSFRTVSFRTAAGALDRGYSLYHVPLRCVREPEPLGFVQCRSASSSSPARPPDTSLFVPLNIKSDYNEDGSVGTELSRPLDKSEALKVLNKFYKRKEIQRLAAENGLDARLFHQAFISFRKFVMESGAIGADLHIVLNDICCGAGHTDDLYPYFMRHAKQIFPMLDCMDDLRKISDLRVPAKWYPEARAIQRKIIFHAGPTNSGKTHHAIQRYLEAKSGVYCGPLKLLAHEIFEKSNSAGVPCDLVTGEERTFMDPEGRQSGHVACTIEMCSVNMPYEVAVIDEIQMIRDPSRGWAWTRALLGLCAEEIHVCGEAAAVNFVTELMYTTGEEVEVRNYKRLTPITLLDHALESLENLRPGDCIVCFSKNDIYSISRQIEARGLECAVIYGSLPPGTKLAQAKKFNDPDDPCKILVATDAIGMGLNLSIKRIIFSSLMKPSVNEKGEKEMDTISTSQALQIAGRAGRFSSVFKEGEVTTMHRDDLLVLKEILGRAVDPIETAGLHPTAEQIEMFAYHLPEATLSNLIDIFVSLSQVDGMYFVCNIDDFKFLADMIQHIPLNLRSRYVFCTAPINKKQPFVCTSFLKFARQFSRDEPLTFSWVCGHISWPLAPPRNIKDLIHLEAVHDVLDLYLWLSYRFMDMFPDASLVRDIQKELDNIIQIGVRNITRLIRASEASAFPSSSSSSGALSGPDEAAPLLSPFSTDRKASYQELWRARAQKPSQRRVRSSKILGFRVEDGQKGLKDSLSSRLIQEGLLTPDLLEQLQREWSSGQPPRWADSGSESGNDSKPSARGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSL1</accession>
    <name>W5MSL1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interleukin-20 receptor subunit beta-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217046.1">
        <property type="nucleotide sequence ID" value="XM_015361560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSL1"/>
    <dbReference type="FunCoup" id="W5MSL1">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011370"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011386.1">
        <property type="protein sequence ID" value="ENSLOCP00000011370.1"/>
        <property type="gene ID" value="ENSLOCG00000009321.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079194"/>
    <dbReference type="KEGG" id="loc:107079194"/>
    <dbReference type="CTD" id="100174902"/>
    <dbReference type="eggNOG" id="ENOG502S2V6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048354"/>
    <dbReference type="HOGENOM" id="CLU_074126_1_0_1"/>
    <dbReference type="InParanoid" id="W5MSL1"/>
    <dbReference type="OMA" id="QGEYERY"/>
    <dbReference type="OrthoDB" id="8704831at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009321">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:interleukin-20 binding"/>
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        <property type="term" value="P:cytokine-mediated signaling pathway"/>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interleukin 20 receptor subunit beta"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
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    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
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    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR20859:SF48">
        <property type="entry name" value="INTERLEUKIN-20 RECEPTOR SUBUNIT BETA"/>
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        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="17"/>
            <end position="123"/>
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    </feature>
    <feature type="domain" description="Interferon/interleukin receptor" evidence="3">
        <location>
            <begin position="145"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011370.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="306" mass="34588" checksum="2874998A524A1458" modified="2014-04-16" version="1">MVLRETLAAAQSSPLYLLMLTSHILAVSPGIEKLPPPQNVEVESTNMKHFLKWSPLPASFGLVSYSVQFQGEFELLYRNGSWEDVFECQLIAHSTCDMTDYIACNVDYNIRVHAQKGGQRSDWASIRQLFNSRQTKLTTPTMTVTAAREFIRVTFAEIPKSIDVILNYWKKGKESKVFQKTITSEENPFHIVNIEEGVMYCLRALAFAESINKKSESTNPQCYFVPSPVPQWLLPTVLCTSIFLALLLLISLRKYYSVLQSACCPKEPLPDVLLQDLLDYKVKTQHSSNRPSEVCDAILVLPAEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSP3</accession>
    <name>W5MSP3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Interleukin-1 receptor accessory protein-like 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">X-linked interleukin-1 receptor accessory protein-like 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. During presynaptic differentiation may regulate both synaptic vesicle accumulation in axon terminals and subsequent axon terminal remodeling.</text>
    </comment>
    <comment type="function">
        <text evidence="22">May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. May activate the MAP kinase JNK. Plays a role in neurite outgrowth. During dendritic spine formation can bidirectionally induce pre- and post-synaptic differentiation of neurons by trans-synaptically binding to PTPRD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the interleukin-1 receptor family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003461">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219526.1">
        <property type="nucleotide sequence ID" value="XM_015364040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSP3"/>
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        <property type="interactions" value="551"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011418.1">
        <property type="protein sequence ID" value="ENSLOCP00000011402.1"/>
        <property type="gene ID" value="ENSLOCG00000009346.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683961"/>
    <dbReference type="CTD" id="568868"/>
    <dbReference type="eggNOG" id="KOG3971">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286976"/>
    <dbReference type="HOGENOM" id="CLU_025552_0_1_1"/>
    <dbReference type="InParanoid" id="W5MSP3"/>
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    <dbReference type="OrthoDB" id="9925886at2759"/>
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        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009346">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045920">
        <property type="term" value="P:negative regulation of exocytosis"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001486">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000220">
        <property type="entry name" value="X-linked interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000004">
        <property type="entry name" value="X-linked interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF22">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="20" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="15 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 26" id="KW-0732">Signal</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866491" description="Interleukin-1 receptor accessory protein-like 1" evidence="26">
        <location>
            <begin position="19"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="46"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="153"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="242"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="27">
        <location>
            <begin position="403"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013309"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00057129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="712" mass="82151" checksum="4507E155957F5CE3" modified="2014-04-16" version="1">MKAPIPLLILLYTSMTKSLKVVSKRGSVDGCTDWSVDYLKYKVLLGEPVRIKCALFYGYIRANYTHAQSAGLSLMWYKSTGHGDFEEPIPFDGVRMSKEEDAIWFRPAELQDVGLYACVLRNSTYCMKVSMSLTVAENDTDLCYNSKMRYFEKGELSKSKEITCPDIEDYVLPQREPNIVWYKECKPKQWRQSIVRRRDTLIIREVREDDIGNYTCELQFGNFVVRRTTELTVTAPLTDKPPKVLFPSENKMNILEVQIGSPANLTCRAFFGYSGDVSPLIYWMKGEKFIEDLDESRVRESDIRIIREHLGEQEVSISLTIDSVEESDLGNYSCYVENGNGRRQASILLHKRELMYTVELAGGLGAILLLLVCLVTLYKCYKIELMLFYRNHFGADEVDGENKDYDAYLSYTKVDPEQWGQETREEERFALEILPDVLEKHYGYKLFIPERDLIPTGSFTNDHYEDPRLLRAYIEDVARCVDQSKRLIIVMTPSYVVRRGWSIFELETRLRNMLVTGEIKVILIECTELRGIMNYQEVEALKHTIKLLTVIKWHGPKCNKLNSKFWKHLQYEMPFKRIEPILAHEQVLDVSEQGPFGELQTVSAISMAAATSTALATAHPDLRSTFHNTYHTQMRQKHYYRSYEYDVPPTGTLPPLTTLGNQHTYCNIPMTLINGQRPQSKSPREQNLEEAHTNSAVLPLLPRETSISSVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSY6</accession>
    <name>W5MSY6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPII-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSY6"/>
    <dbReference type="FunCoup" id="W5MSY6">
        <property type="interactions" value="1572"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011495"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011512.1">
        <property type="protein sequence ID" value="ENSLOCP00000011495.1"/>
        <property type="gene ID" value="ENSLOCG00000009415.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006916"/>
    <dbReference type="HOGENOM" id="CLU_008658_3_0_1"/>
    <dbReference type="InParanoid" id="W5MSY6"/>
    <dbReference type="OMA" id="FPPHYAE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009415">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048702">
        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035138">
        <property type="term" value="P:pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000003">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000003">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000011">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF7">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="14">
        <location>
            <begin position="58"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="15">
        <location>
            <begin position="126"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="17">
        <location>
            <begin position="402"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="16">
        <location>
            <begin position="519"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="13">
        <location>
            <begin position="633"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="766" mass="86177" checksum="D8B825D3DD6E2F50" modified="2014-04-16" version="1">MATFQEFIQQNEDRDGVRFSWNVWPSSRLEATRMVVPVATLLTPLKERPDLPPIQYEPVLCSRATCRAVLNPLCQVDYRAKLWACNFCYQRNQFPPTYAGISEMNQPAELLHQFSTIEYVVQRGPQMPLIFLYVVDTCMEDEDLQALKESLQMSLSLLPPTALVGLITFGRMVQVHELGCEGISKSYVFRGTKDLSAKQLQEMLGLTKPAAAQAGRGVQVQQPPPSNSRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSIAVGLLECTFPNTGGRIMMFIGGPATQGPGMVVGDELKTPIRSWHDIEKDNAKFMKKATKHYEALASRASNSGHIIDIYACALDQTGLLEMKCCTNYTGGYMVMADSFNTSLFKQTFQRVFTKDVQGSFKMAFGGTLEIKTSREIKISGAIGPCVSLNAKGPCVSENEIGTGGTCQWKICGLDHNSTLAVYFEVVNQHNAPIPQGGRGAVQFVTQYQHSSGQRRIRVTTIARNWADAQTQIQSIAASFDQEASAILMARLAVFRAESEEGPDVLRWLDRQLIRLCQKFGDYHKDDPNSFRFSETFSLYPQFMFHLRRSPFLQVFNNSPDESSYYRHQFMRQDLTQSLIMIQPILYAYSFNGPPEPVLLDSSSILPDRILLMDTFFQILIYHGETVAQWRKAGYQDMPEYENFRHLLQAPVDDAQEILHSRFPMPRYIDTEHGGSQARFLLSKVNPSQTHNNMYAWGQESGAPILTDDVSLQVFMDHLKKLAVSSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUJ9</accession>
    <name>W5MUJ9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUJ9"/>
    <dbReference type="FunCoup" id="W5MUJ9">
        <property type="interactions" value="988"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012058"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012079.1">
        <property type="protein sequence ID" value="ENSLOCP00000012058.1"/>
        <property type="gene ID" value="ENSLOCG00000009866.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162569"/>
    <dbReference type="HOGENOM" id="CLU_014184_6_0_1"/>
    <dbReference type="InParanoid" id="W5MUJ9"/>
    <dbReference type="OMA" id="FMAIQAM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009866">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
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        <property type="project" value="AgBase"/>
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        <property type="term" value="C:synapse"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="F:G protein-coupled receptor binding"/>
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        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
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        <property type="term" value="P:entrainment of circadian clock"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
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    <dbReference type="GO" id="GO:0007213">
        <property type="term" value="P:G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007603">
        <property type="term" value="P:phototransduction, visible light"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF364">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN (G PROTEIN), Q POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="361" mass="42373" checksum="DDCA36AE84F8BA5C" modified="2014-04-16" version="1">GRMTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQSMIRAMDTLKIPYKYEHNKGNAHTVREVDVEKVSVFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRIADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUT1</accession>
    <name>W5MUT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Antizyme inhibitor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ornithine decarboxylase antizyme inhibitor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Interacts with OAZ1 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUT1"/>
    <dbReference type="FunCoup" id="W5MUT1">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012140"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012161.1">
        <property type="protein sequence ID" value="ENSLOCP00000012140.1"/>
        <property type="gene ID" value="ENSLOCG00000009927.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696000"/>
    <dbReference type="KEGG" id="loc:102696000"/>
    <dbReference type="CTD" id="317764"/>
    <dbReference type="eggNOG" id="KOG0622">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182995"/>
    <dbReference type="HOGENOM" id="CLU_026444_1_1_1"/>
    <dbReference type="InParanoid" id="W5MUT1"/>
    <dbReference type="OMA" id="AMKNFSM"/>
    <dbReference type="OrthoDB" id="5034579at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009927">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042978">
        <property type="term" value="F:ornithine decarboxylase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042177">
        <property type="term" value="P:negative regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902269">
        <property type="term" value="P:positive regulation of polyamine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000008">
        <property type="entry name" value="antizyme inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000010">
        <property type="entry name" value="Antizyme inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF7">
        <property type="entry name" value="ANTIZYME INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="11">
        <location>
            <begin position="45"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="449" mass="49226" checksum="8957077557D45ED9" modified="2014-04-16" version="1">MKGFVDEPNYTIDLLEDGTTLGDVIDNHICEQALAEKNAFFVADLGDIVKKYTLWQNVMTQIKPFYAVKFNNSQAVIEILAALGAGFACTNKSEVALVLSCGVAPENILFTSLCKQLSHVTNAAKNGIDILVCDNETELRKIARCHPNARLLLQIATETHSEGEEMSMAFGSTLKNCRHLMESAKERGVQVVGVKFHISSSCKDMQAYTHALSDARCVFDMGEEFGFNMNILDIGGGFCGSEAQLEEIYRSMRPLLDVYFPPESGVSVIAEPGSYYVASSFTLAVNIIAKKVVARDQYDHPLDVEPSLNDEPAFVYYMNDGVYGSFASKLLEKAIAAPSVHKKSSKEEPVFASSLWGPSCDGLDQVVEHCLLPELEVGDWVIFNNMGANAMGGQSIGDFQKPPIYYLISACDWYEMQDAGVTLESAMKNFLAIPSCFQLGQEDRISAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV62</accession>
    <name>W5MV62_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ankyrin repeat and sterile alpha motif domain containing 1A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012271"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012292.1">
        <property type="protein sequence ID" value="ENSLOCP00000012271.1"/>
        <property type="gene ID" value="ENSLOCG00000010031.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155806"/>
    <dbReference type="HOGENOM" id="CLU_010379_0_0_1"/>
    <dbReference type="InParanoid" id="W5MV62"/>
    <dbReference type="OMA" id="GKANVCR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010031">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09499">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09500">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000099">
        <property type="entry name" value="ankyrin repeat and sterile alpha motif domain-containing protein 1B isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041880">
        <property type="entry name" value="SAM_ANKS1_repeat1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041882">
        <property type="entry name" value="SAM_ANKS1_repeat2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF4">
        <property type="entry name" value="ANKYRIN REPEAT AND SAM DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="686"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="757"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="929"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="839"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1069"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="586"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="666"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="839"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="862"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="887"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1168" mass="127410" checksum="A8A4326A6EC80211" modified="2014-04-16" version="1">MGKEQELLEAARTGNLAAVEKLLSGKRQSAGIGGGSSGGGGSGGGGGGGHSSSSHPLSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHSEVVEALLRNEALTNIADNKGCYPLHLAAWKGDQQIVRLLIHQGLSHPKLNEQSCVDHKEFKRCGPFDPYINAKNNDNETALHCAAQYGHSEVVRLLLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLNAHPNLLSCNTKKHTPLHLAARNGHLAVVEVLLDAGMDINYQTEKGSALHEAALFGKKDVVQKLLSAGPCFNMVXPPILRGCGSVKLFPLLQVAVSIFLIEGKDSKRERPPPPSKPPPDQDEDHTGSEMRNIGLGSIRVVLGPPRPTRGYAPASSCLTLMCITQHTNSFSKNHKRQSDAAGSFSTLVPLHVNTLQEQTTKPTPPLPVVSEHSLSNFFSTRCVTGLYVNSRIADREGEEDHPYELLLSAKTKIPPGQEPPLRTESYIFNSDGDQAGGIPEQFSGLLHGSSPVLESKEEPFTVPGEAPLASQEPRTVTGEGQGEVAPPAGAAMQGPQVPIDPKAIYATVVHTKPDSREGGTLPSGRSRPPGDLKLARSLSKSDSDLLVSPPGEEDGLGSRSESVSNCSRGKKRLEKSPSFASEWDEIEKIMTLIGAGIEFSREQQPLASGGGGSGGGGAGGSRALDQPVGDWLEHLGLPQYESKLLLNGFDDLRFMGSNVMEDQDLKEIGILDQHHRRKILQAARSLPKVKALGCDGSTSLASWLDVLGLQEYLQNFLASGYRTIDSVKNLWELEIVNVLKINLLGHRKRIIASLAERPYEEPPIKPPRLSQIRCQDLVSQTSSPLSHDSYTGRSTDMLLAPGDSGKRRTEHEHDVPHRSRSERHHRSHDRHDDRPREPRLTLRPPSLATPYAPVQNWQHQPEKLIFESCGYEASYLGSMLIKELRGTESTQDACAKMRRSTEQMKKVPTIVLSITYKGVKFIDAANKRLDHRNLIRLFAVYLSPVSKNPPKDLTGLTFLTKDLQTPYSPVYIHKAFSQNLTYEIILTLGQAFEVAYQLALQAQRTKPHGVTSATGSEVIETKSTRPVPKPRGSVRKSAGAPLLESRCCYCHTCTTHRPSYLPLRSVSPGAQMEPLELETDSQSQGSATWLVDQKTDSKRTISTKYETTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW22</accession>
    <name>W5MW22_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein phosphatase, Mg2+/Mn2+ dependent, 1J</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW22"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012581"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012605.1">
        <property type="protein sequence ID" value="ENSLOCP00000012581.1"/>
        <property type="gene ID" value="ENSLOCG00000010272.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689250"/>
    <dbReference type="KEGG" id="loc:102689250"/>
    <dbReference type="CTD" id="333926"/>
    <dbReference type="eggNOG" id="KOG1323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166858"/>
    <dbReference type="HOGENOM" id="CLU_029072_2_0_1"/>
    <dbReference type="InParanoid" id="W5MW22"/>
    <dbReference type="OMA" id="RDHSMTG"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010272">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
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        <property type="entry name" value="PPM-type-like_dom_sf"/>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF849">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="78"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="492" mass="54659" checksum="E2351410E1D2CFCB" modified="2014-04-16" version="1">MISKVKNAMSTLVGGIMPHGHQHGSASIQHSGSEGLPVRFPYSRPDFLDLPVDQLQYANDHGSRPILTPQRDCRLPWRTGYAEVVNAGKSLLNEDQACSEVLFLRRSNVKQPSSSSVEEEAQQVGIPLHFWALYDGHAGSGAAIMASKFLHHVIKDQLTDILHLLENPDTAPPICLGKNGSPLQPELKTVASQGEDPETPSDPTTRFHLEKAVSLESLIMGTIETAFKQMDEQIEKEKASFSISGGCCALVVIHLMGKLYVANAGDSRAIIIHNDEVIPMSSEFTPETERQRLQYLGFLKPELLGNEFTHVEFPRRMQHKELGKKMLFRDHTMTGWAYKTIHEEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVYNSNIYIKPFLSCCPEVKVFDMTQHEFGPNDVMVLGTDGLWDVTTNREVADAVTALLSHYKPNDPLRYTLAAQDLVMRSRGVLKERGWRLANDKLGSGDDISVFVIPLNGHAAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWN2</accession>
    <name>W5MWN2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 21">5-hydroxytryptamine receptor 2A</fullName>
            <shortName evidence="21">5-HT-2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 21">Serotonin receptor 2A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">G-protein coupled receptor for 5-hydroxytryptamine (serotonin).</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Cell projection</location>
            <location evidence="1 21">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 21">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 21">Membrane</location>
            <location evidence="2 21">Caveola</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWN2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012791"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012816.1">
        <property type="protein sequence ID" value="ENSLOCP00000012791.1"/>
        <property type="gene ID" value="ENSLOCG00000010438.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102696873"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244937"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_3_1"/>
    <dbReference type="InParanoid" id="W5MWN2"/>
    <dbReference type="OMA" id="MVTIGIH"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010438">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000455">
        <property type="entry name" value="5HT2A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF30">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00516">
        <property type="entry name" value="5HT2ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0085">Behavior</keyword>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17 21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0770">Synapse</keyword>
    <keyword evidence="15 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="91"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="469" mass="52224" checksum="EB7CF006ECF9AA37" modified="2014-04-16" version="1">MNFSEVSSNSSNLTSVSFVPLNLDRRPQKPEFLLGKANGSHPCNTSMDARNLSDLSCAEGPAPQCFSMVSLLDKNWPALLTIAVIVLTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYIWPLPSELCPIWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKAFMKIIAVWTISVGISMPVPVFGLRDNSKVLKDGICFLADDNFVLIGSFVAFFIPLTIMVVTYFLTIHALQNEATMCLDELVPKPRWTSFSFLQQSSLSSEKLFRRSLSREAGPYGRRTMQSISNEQKASKVLGIVFFLFVVMWCPFFITNVLAVICKDTCDSNVIGGLLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKEEKKPLQLILVNTLPPLAYNSTQLQLGELSSLKNSSRMGGKDYSLVAIGINNFNTSKPLSGQASEKVSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXC0</accession>
    <name>W5MXC0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SREK1IP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Possible splicing regulator involved in the control of cellular survival.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192425.1">
        <property type="nucleotide sequence ID" value="XM_015336939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXC0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013057.1">
        <property type="protein sequence ID" value="ENSLOCP00000013029.1"/>
        <property type="gene ID" value="ENSLOCG00000010643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692532"/>
    <dbReference type="KEGG" id="loc:102692532"/>
    <dbReference type="CTD" id="285672"/>
    <dbReference type="GeneTree" id="ENSGT00510000047710"/>
    <dbReference type="HOGENOM" id="CLU_122662_0_0_1"/>
    <dbReference type="OrthoDB" id="5596742at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010643">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437:SF1">
        <property type="entry name" value="PROTEIN SREK1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437">
        <property type="entry name" value="SREK1IP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13917">
        <property type="entry name" value="zf-CCHC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="17930" checksum="F109C89A419DF282" modified="2014-04-16" version="1">MALPGPNKDNIRAGCKKCGYPGHLTFECRNFVRVDPRKDIVLDVSSTSSEESEEEEQEVLNERVQASSTAKSSHEEPKGGQQKKKSKDKSHKKKIKRSGTSSDEEEHHKRKKHKIHKKKRKGEKKHKKEKQHKKEGKKTKKRSHSSSSDSSSISSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXX8</accession>
    <name>W5MXX8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ring finger protein 103</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629769.1">
        <property type="nucleotide sequence ID" value="XM_006629706.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXX8"/>
    <dbReference type="FunCoup" id="W5MXX8">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013266.1">
        <property type="protein sequence ID" value="ENSLOCP00000013237.1"/>
        <property type="gene ID" value="ENSLOCG00000010801.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683035"/>
    <dbReference type="KEGG" id="loc:102683035"/>
    <dbReference type="CTD" id="7844"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006413"/>
    <dbReference type="HOGENOM" id="CLU_031351_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXX8"/>
    <dbReference type="OMA" id="PDWLAWP"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010801">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16473">
        <property type="entry name" value="RING-H2_RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042494">
        <property type="entry name" value="RNF103"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15302">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15302:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="613"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="675" mass="78314" checksum="EFECDA0418D4D9D6" modified="2014-04-16" version="1">MWLKLFFLLLYFLVLFVLARFFEAVVWYETGIFATQLVDPVTLSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKSEEEASDPVSSTSFSGEMHFYELVEDTKDGIWLVQVIAKDRDPLVSKTNWGKMVKKVSHFGIRTGTFNCSNDSRYCRKRGWLKSTLIMSVPQTNTSKGKVMLKEYNGNRIETEHIFKWMTAHVASRIKTVRLAEQLVAEWNRSDRDQVKMYLFAKLDQPPAFFSALSVKFTGRIEFIFVDVQNWSNESYMKEIGVQQIPSYILKMPEGTYRYGNSTGEFISLSAMDTFLRSVQPEVNDLFVLSLVMVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPILGFLQLPYLDSFYEYSLKLLRYADTTTIASWVRADWSFYSSHPALFLSTYLGHGLLIDYFEKKRRRNNNDDEINANNLEWLSSLWDWYTSYLFHPIASFQNFPNESDWDDDPNLLLERLAFPDLWLHPLIPTDYIKSLPTWNFKCTRAQSCDREPNAGSWRESDRELESELRSELEPSDDEWCGPEKERTPPRACVCKNVENGSCKNESDCPGNLNAKEDLEPDWSKWPNHMLHCTECVVCLENFENGCLVMGLPCGHVFHQQCIVVWLAGGRHCCPVCRWPSYKKKHRRQDQEAQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ89</accession>
    <name>W5MZ89_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SUMO-conjugating enzyme UBC9-B-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ89"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013698"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013698.1"/>
        <property type="gene ID" value="ENSLOCG00000011151.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102683539"/>
    <dbReference type="eggNOG" id="KOG0424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075088"/>
    <dbReference type="HOGENOM" id="CLU_2311003_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ89"/>
    <dbReference type="OMA" id="YVLFTQN"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011151">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0061656">
        <property type="term" value="F:SUMO conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000172">
        <property type="entry name" value="SUMO-conjugating enzyme UBC9-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="120" mass="13585" checksum="441D9A1486C47934" modified="2014-04-16" version="1">MSGIALSRLAQERKTWRKDHPFGFVAVPTKNPDGTLNLMNWECAIPGKKGTLWEGGQYKLRMLFKDDYPSSPPKCEYFLGMGEGRELIKLAYSEETAKERFISTARSPAPADHEQSAQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N092</accession>
    <name>W5N092_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cathepsin La</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017174">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N092"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014051"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014080.1">
        <property type="protein sequence ID" value="ENSLOCP00000014051.1"/>
        <property type="gene ID" value="ENSLOCG00000011433.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153321"/>
    <dbReference type="HOGENOM" id="CLU_012184_1_2_1"/>
    <dbReference type="InParanoid" id="W5N092"/>
    <dbReference type="OMA" id="VYYDEEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011433">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000332">
        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018676796" evidence="7">
        <location>
            <begin position="20"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="9">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="8">
        <location>
            <begin position="120"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="341" mass="38665" checksum="9E599645A7872F7E" modified="2014-04-16" version="1">STMHLDLSLLLLCFSAVSAAPSLDPQLDEHWELWKNWHSKKYHEKEEGWRRVVWEKNLKKIELHNLEHSLGKHSFRLGMNHFGDMTTEEFRQLMNGYKRKPSVERKLKGSMFLEPNFLEAPRQVDWREKGYVTPVKDQGQCGSCWAFSATGALEGQQFRKTGKLVSLSEQNLVDCSRPEGNEGCNGGLMDQAFRYVKDNSGLDSEESYPYIGTDDQPCRYDPQNSAVNDTGFVDIPSGKEHALMKAVAAFGPVSVAIDAGHQSFQFYQSGIYYEKECSSQDLDHGVLAVGYGFEGKDEDGKKYWIVKNSWSDKWGNKGYIYMAKDRNNHCGIATAASYPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3V8</accession>
    <name>W5N3V8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heart- and neural crest derivatives-expressed protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3V8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015317"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015346.1">
        <property type="protein sequence ID" value="ENSLOCP00000015317.1"/>
        <property type="gene ID" value="ENSLOCG00000012444.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161111"/>
    <dbReference type="HOGENOM" id="CLU_119591_0_0_1"/>
    <dbReference type="InParanoid" id="W5N3V8"/>
    <dbReference type="OMA" id="QIQAPYT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012444">
        <property type="expression patterns" value="Expressed in heart and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18952">
        <property type="entry name" value="bHLH_TS_HAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF3">
        <property type="entry name" value="HEART- AND NEURAL CREST DERIVATIVES-EXPRESSED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="76"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="191" mass="21739" checksum="52F624F362F0C0BC" modified="2014-04-16" version="1">DMNLIGSYQHHHLMREPFPFVSRCHQDNPYFQSWVVNHGEVTPELHLQPSYGSEFVVPGPAPEQGQLEALDGRMGRRRVSAPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAGETEGFKAEIKKFDNRDLKRKREANEGVPGSLAGEKRLKGRTGWPQQVWALELNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3X6</accession>
    <name>W5N3X6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G protein-coupled receptor 176</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3X6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015335"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015364.1">
        <property type="protein sequence ID" value="ENSLOCP00000015335.1"/>
        <property type="gene ID" value="ENSLOCG00000012456.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182998"/>
    <dbReference type="HOGENOM" id="CLU_045778_0_0_1"/>
    <dbReference type="InParanoid" id="W5N3X6"/>
    <dbReference type="OMA" id="VTDIYAM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012456">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15006">
        <property type="entry name" value="7tmA_GPR176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043523">
        <property type="entry name" value="GPCR_176_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="80"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="534" mass="60237" checksum="0BD70FD957387863" modified="2014-04-16" version="1">KMEEDNMGSWDSITGNGSEFFTPANRIMDFQNTSNSVLYWDNKTTKEILPLERQRYSQEQTYRDFTTSMQVVIFLGSLLGNAMVLWSTCRTTVFKSVTNRFIKNLACSGICANLVCVPFDIVLSASPRCCWWVYTLLFCKVIKFLHKVFCSVTILSFTAIALDRYYSVLYPLERKISDAKSRDLVIYIWVHALVASAPVFAVTNVTDIYAMSMCTDSLSYSLGHLVYVIVYNVTTVILPVAVVFFFMLLIRRALSASQKKKVIIAALRTPQNTISIPYVSQREAELHSMLLSMVLTFIVCSIPYMMLIVYRTILNISEISVFLLLTAIWLPKVSLLTNPLLFLTINKSVRKCLVGTIAQLHRRYSRRNIVNSGGIADATAEPNVRSGSQLLEMFNIGQQQIFKPTEEDEENETKSIGSGDFRPKAIPSTSLEIKQAAVQKFSPPHSSTDSAAQVAPATPSDVEDVNDKYATHFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKQPKLRTERKISRNNKVSIFPRVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5J6</accession>
    <name>W5N5J6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Charged multivesicular body protein 6a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635228.1">
        <property type="nucleotide sequence ID" value="XM_006635165.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5J6"/>
    <dbReference type="FunCoup" id="W5N5J6">
        <property type="interactions" value="1436"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015905"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015935.1">
        <property type="protein sequence ID" value="ENSLOCP00000015905.1"/>
        <property type="gene ID" value="ENSLOCG00000012913.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691623"/>
    <dbReference type="KEGG" id="loc:102691623"/>
    <dbReference type="CTD" id="445397"/>
    <dbReference type="eggNOG" id="KOG2910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00720000108863"/>
    <dbReference type="HOGENOM" id="CLU_086201_3_0_1"/>
    <dbReference type="InParanoid" id="W5N5J6"/>
    <dbReference type="OMA" id="RAKQPAM"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012913">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="204" mass="23784" checksum="E59DD10EEB1F630C" modified="2014-04-16" version="1">MGNIFGRKRRTRVTEQDRAVLQLKQQRDKLKQYQKKISLHLEKERLLAKQLLRDGKKEKALLLLKKKRYQDQLLDKTENQISNLEHMVQDIEFAQIEMKVIEGLKVGNECLKKMHEVMSIEEVERIMDETQDGIEYQRQIDDMLAGSLTQEDEDAVLAELEAISQGEEVELPDVPSERLPEVPEKEPEKERVKNKPKPELVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N695</accession>
    <name>W5N695_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkey-177p2.18</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N695"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016154"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016184.1">
        <property type="protein sequence ID" value="ENSLOCP00000016154.1"/>
        <property type="gene ID" value="ENSLOCG00000013113.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063883"/>
    <dbReference type="InParanoid" id="W5N695"/>
    <dbReference type="OMA" id="FCDAEHG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013113">
        <property type="expression patterns" value="Expressed in intestine"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF45">
        <property type="entry name" value="PHOSPHOLIPASE B1, MEMBRANE-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867309" evidence="1">
        <location>
            <begin position="21"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="437" mass="49118" checksum="4CE3912A900AC927" modified="2014-04-16" version="1">MRHLSLLLAVCVVCSSSVQGSNWMKKYEDGLKGYSEESLAQEFPEKIRPQNYKHPLFRCPDMSPSSSVPTSVQRVKPADIKVIAALGDSLTTAIGANASSILGIPNEYRQVSWSIGGYGTYQDVITLANIVKLFNPNVIAPALKRTVNGKPATLKETGFNLAVTGHNTYEFTQQTRHLIDTLKNYPGLSFEEDWKLLTILIGNNDICDYCKNKTLYSADSFIHNLTVALDMLYNEIPRMIVNLVEVLDMEGLREVNDGSIGCLLQSRSFCSCLVRPDTGSPELEELIELNHEFQRRMEQLIGSGRYNKDDFSVVLQPYLKKAKPPKDESGKVDYSFFAPDCFHFSIKGHEELAKGLWNNMFQPDGEKFEIENFSEPIELICPPEDHPYIYTTGHVRNTASFNIESPEIPIFLNVLHLTVPVAKIVPVFAFLIILELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6B2</accession>
    <name>W5N6B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Glypican 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <topology evidence="1 12">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015195798.1">
        <property type="nucleotide sequence ID" value="XM_015340312.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6B2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016171"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016201.1">
        <property type="protein sequence ID" value="ENSLOCP00000016171.1"/>
        <property type="gene ID" value="ENSLOCG00000013124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690053"/>
    <dbReference type="KEGG" id="loc:102690053"/>
    <dbReference type="CTD" id="221914"/>
    <dbReference type="eggNOG" id="KOG3821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244897"/>
    <dbReference type="HOGENOM" id="CLU_024658_2_1_1"/>
    <dbReference type="InParanoid" id="W5N6B2"/>
    <dbReference type="OMA" id="WNHFVEA"/>
    <dbReference type="OrthoDB" id="10010764at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013124">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019803">
        <property type="entry name" value="Glypican_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF24">
        <property type="entry name" value="GLYPICAN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01207">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869331" evidence="14">
        <location>
            <begin position="24"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="333"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="472"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="578" mass="64717" checksum="87D11B2163499B1A" modified="2014-04-16" version="1">MALRLPAPCLLALLLALCRGAAGGRSCSEVRLAYGAKGFSLATAPQTQISGEHLRLCPQDYTCCSSEMEEGLARQSETDFLAAVDETSNFLLTTFTQRHRKFDEFFRELIDVAEKSMNQMFTKTYGRLYTQNAAVFQDLFTELRRYYTGGSVSLGEVLSDFWARLVERVFSLVNPQYQFSDEYLECVSKHAEQLQPFGDLPRKLRIQVSRAFIAARALVQGLATGRDIASKAAKPSPSRECVRALMRMSYCPLCRGLPTLRPCHAFCRNVMKGCLANQADLDAEWNNFIDALMQVAEKLEGPFNMELATDSIAIKISEAIMFMQENSVQTSTKVFQGCGSPRPAPARTRRSPGREEGKRRFRTYSPEEKPTTAAGTNLDRLVADLKERLRPMKRFWVTLPHTLCNDEKMAADVTNEEKCWNGQGRGRYLPDVTGDGLVNQINNPEVEVDIARPDVKTRQQIMELRVATNRLRQAQSGHDSDFQDSSEDGSGSGTGERYSEDWPGYGPNTYNYGKPPRTDPGQSKPPRTRDRPKWTRPNQHQNSGSRVKATGAGPPTVQSTLILPFACSLFLLGAPLWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7D9</accession>
    <name>W5N7D9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat domain 72</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7D9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016548"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016578.1">
        <property type="protein sequence ID" value="ENSLOCP00000016548.1"/>
        <property type="gene ID" value="ENSLOCG00000013418.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160298"/>
    <dbReference type="InParanoid" id="W5N7D9"/>
    <dbReference type="OMA" id="YHCSSRM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013418">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000247">
        <property type="entry name" value="WD repeat-containing protein 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049916">
        <property type="entry name" value="WDR72-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057848">
        <property type="entry name" value="WDR72_alpha-sol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099">
        <property type="entry name" value="RABCONNECTIN-3B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44099:SF2">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23123">
        <property type="entry name" value="WDR72_alpha-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="58"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="464"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="566"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="WDR72-like alpha-solenoid" evidence="5">
        <location>
            <begin position="887"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1112" mass="122990" checksum="D4A1DD337EA1EEA7" modified="2014-04-16" version="1">MKSSEQYLTMWSKKPPSHSITAIMLADDQATIVTGSREGQICLWDLSSDLKISPKALLFGHTASITCVAKASEFDKKPYVVSASNNGEMSLWNVYSGECVESTLLPYVHIAICYYYCSSRVAGDGWLLCCGEYQDVLLIDAQTLQVLHKFFSRDSSDWISSLCVAHSLKTQEDSLLAISVTGVLKVWSLSSSGNSVQEKSAIYESESRSLQCFQCQGVSFCKYTESRLLVVCSDRWQVYDYSDFSLLCVVLSNAGQCFAGGELLPANRLIVWSKDRDSYIYQLSDRWAALLKVQLGSTSPLYRTAGDEERNLTSKEISAHILCYTSERKVSAQVMGFLSERKEPFYQILFCGDEQGKVTLWHLPDVPRLNSNGLPEEIVESAECRFEGCLEKNVPGEITGPLGGHSEGLEMITVTASVSIPSYHQLVCGCDNGKIIVKLALQAAQAQLLGGSSQLKDDQPLKTLQGHSGAVTSLLYPYCKSERFGSSWLLSGGQDSRVIWWDLLTGEILHTFTLQAGPVKKLLVLSENLVESDYQLPKVHLRVCCFATDNSIAILNLDGPACILHVRKHLFSVNAILWRPVEDFLIVSCVDGSVYVWEIESGTLDRHETGEVAKTILSSCDESQALLIDTGVPVPTAARPSRLHKFMQQKGCNSHKNIILPALSITIDNNCKISCHVTAFNPFSLVPLEAGPLNSGTHILTFYVEEILEKLDQASQDGTIALNTFHSFRKPKREPAFDKPATSLKRSKTIFSLQHVNGHLQTFVRDHLFQSERSLDHFPTGEVRLARQASQPVSTRGRRQQPKRLVNINLARDSAGLLLSGLFPWGTDKELDDCCARELGIIPLLTSKPFCLKSKNGHMSLILPGWHPGRLGHIVEGQPSNLIAYRVVELGKQYASSITAEIPLLEAFEDAKKDAFRLKVVSYLLSVVLVVDSLMRLTSPHGQDRNSNTKMFVVEWKIQRNGPDLLMANYLKPAREAAQYLVREASSLEKLVTSWQNQSVEVLEAVQAVLLAEVQRIIKTLRKTSISSQTPTVADSGSCEMQLPKIEGMELLELQHTQKIAPQTPVGLVKHDSGSGLAKGQAQEEVSDGCIFEDSDSTGGLSREQFPCCKMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N822</accession>
    <name>W5N822_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyribonuclease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNase I family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631146.2">
        <property type="nucleotide sequence ID" value="XM_006631083.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N822"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016781"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016811.1">
        <property type="protein sequence ID" value="ENSLOCP00000016781.1"/>
        <property type="gene ID" value="ENSLOCG00000013612.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685189"/>
    <dbReference type="KEGG" id="loc:102685189"/>
    <dbReference type="CTD" id="445317"/>
    <dbReference type="eggNOG" id="ENOG502R3BK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182846"/>
    <dbReference type="HOGENOM" id="CLU_043335_2_1_1"/>
    <dbReference type="InParanoid" id="W5N822"/>
    <dbReference type="OMA" id="NSKHHYT"/>
    <dbReference type="OrthoDB" id="10061407at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013612">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004530">
        <property type="term" value="F:deoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10282">
        <property type="entry name" value="DNase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000007">
        <property type="entry name" value="Deoxyribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016202">
        <property type="entry name" value="DNase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371">
        <property type="entry name" value="DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371:SF26">
        <property type="entry name" value="DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000988">
        <property type="entry name" value="DNase_I_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00130">
        <property type="entry name" value="DNASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00476">
        <property type="entry name" value="DNaseIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="11"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000988-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="303" mass="35125" checksum="B2BF66F572CE8EC9" modified="2014-04-16" version="1">MHKKSEVMKIASFNIQRFGENKIADKNILSVLIKTVSRYGIILILEVVDVKGKAIKKFLEELNKANKDNPYTMQISTRLGRHTYKEQFMFLYREDLVKLTGSYQYEDNQVGDEDAFAREPFVLRFTCSKTVLKDLVLIPVHTQPSSVEKELDELYDVFLAVQDKWETDNVMMLGDFNADGGYLSNKKMKKIRIRSDKNFHWLIEDEVDTTANKGNDNTYDRIVVYGDDMLDAIVPYSAKPFNFQEEFQLTDEMTLKVSDHYPVEVELKATNSQDRRPGNKRPKRTPQTQPAAHPRAGKKPRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8J1</accession>
    <name>W5N8J1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Caspase-3</fullName>
            <ecNumber evidence="10">3.4.22.56</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.</text>
            <dbReference type="EC" id="3.4.22.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.56" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01002146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8J1"/>
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    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
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        <location>
            <begin position="30"/>
            <end position="154"/>
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            <begin position="169"/>
            <end position="263"/>
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            <dbReference type="RuleBase" id="RU003971"/>
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            <dbReference type="PROSITE" id="PS50208"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="263" mass="29623" checksum="D2D7A832BF819264" modified="2014-04-16" version="1">SSQGPAVPPKQVDAKQHDSHTYRYKMNYPHLGQCIIINNKNFDKKTGMNIRNGTDVDAGNMMKVFKMLGYSVRVVNDQTCAQMETCLQSISREDHSKAASFVCVLLSHGDEGVLFGTDGCLEIKKLAGYFRGDRCRTLVGKPKLFFIQACRGTSLDGGIETDALGDDNSSEKLPVEADFLYAYSTAPGYYSWRNQVRGSIFIRRLCEMLDQHGRDLDLARILTRVSHWVALRFESDTGAWLSHGKKQMPCIISRLTRDLYFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9D4</accession>
    <name>W5N9D4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Progestin and adipoQ receptor family member Va</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="510"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017243"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017274.1">
        <property type="protein sequence ID" value="ENSLOCP00000017243.1"/>
        <property type="gene ID" value="ENSLOCG00000013987.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158844"/>
    <dbReference type="HOGENOM" id="CLU_052356_1_0_1"/>
    <dbReference type="InParanoid" id="W5N9D4"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013987">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 10 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:signaling receptor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF38">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="97"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="280"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="368" mass="42050" checksum="5F919929065CF12F" modified="2014-04-16" version="1">MLSLKLPRVFSINQVPKVFHEDSIISGYRQPCSSATDCVLSVFQLTNETLNIWTHFLPTWYFLWKLLTVVFMQDVWHDAFTWPLVVFLASCCMYPLASSCAHTFSTMSTRARHICFFFDYGALSFYSLGSAIAYSAYVIPDRWIGSTFSRYYVPIAVFNTVLCTSLACYSRLGSPFLHYNHDVTKRFPDEKRDYFRLPECERPKLSKALRVVAFAYPYLFDNIPVFYRIFFCVGEGCTDNETNLLHYRHMALAFLTGFLFATHLPERLAPGCFDYIGHSHQLFHVCGILGTHYQVESIVMDMAQRRQWLLAHGPPVTFASSFGAGLLCVVLSLGIICAFSLPLFWAPAAPRKQKRSRRPRAAPKASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAL0</accession>
    <name>W5NAL0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small EDRK-rich factor 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SERF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5NAL0"/>
    <dbReference type="FunCoup" id="W5NAL0">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017669"/>
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        <property type="protein sequence ID" value="ENSLOCP00000017669.1"/>
        <property type="gene ID" value="ENSLOCG00000014349.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_165034_1_1_1"/>
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        <property type="component" value="Linkage group LG3"/>
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    <dbReference type="Bgee" id="ENSLOCG00000014349">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
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    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
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    <dbReference type="InterPro" id="IPR040211">
        <property type="entry name" value="SERF1/2-like"/>
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    <dbReference type="PANTHER" id="PTHR13596:SF0">
        <property type="entry name" value="SI:CH211-39K3.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13596">
        <property type="entry name" value="SMALL EDRK-RICH FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="57" mass="6591" checksum="6B8BC61D505F3CEB" modified="2014-04-16" version="1">GNQRELARQKNAKKQNEQSRGKRNEDGLSAAARKQRDAEIMQQKQKKANEKPKEQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND60</accession>
    <name>W5ND60_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tubulin tyrosine ligase-like family, member 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND60"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018569"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018601.1">
        <property type="protein sequence ID" value="ENSLOCP00000018569.1"/>
        <property type="gene ID" value="ENSLOCG00000015088.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006760"/>
    <dbReference type="HOGENOM" id="CLU_018324_0_0_1"/>
    <dbReference type="InParanoid" id="W5ND60"/>
    <dbReference type="OMA" id="WTPDCKR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015088">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
        <property type="entry name" value="TTLL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="2">
        <location>
            <begin position="92"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="647" mass="74280" checksum="20F150DAC0255FCD" modified="2014-04-16" version="1">ACERGGGCCSGGQTGTSAGMSEEGDAYELFLSLHGAALKASRIPPVYWRSLHYKLTNEVFDAGQVFGIMQVEQVEGDEDEEEDEEAREEGKKKPNPGVQLVSKVIVTSESGLQAADPNSVFLVDHAWTFRVQGAREQLLQVPGLLQRMASLMHIDFHGEFPDEGVVEQVLADMWKFNQTYQLSQGTAEEKVPVWYIVDEFGSQVQHSDEPTCCMAPFFHMPEQVAYTVLWPLQDLDNGDEVTRDYAYGETDPVIRKCRLLPWEPADLRSISSSTPEFPDLYYETISWENKETLPAQIEPQVYPEDKILKVYTEMRQVSDNLSHPRFELTDEEPEADVLWMYSHIRDYRSLSGERPHVLLNQFPCETIVTVKDCLASVARRAGGGRGAPWLPRTFNLQTELPQFISHYQQNQDRGEDNHWICKPWNLARGLDTHITDNLNYIIRQRESTPKVVCKYLESPVLFEREDVGLVKFDIRYIVLLRSVRPLQLYAYDVFWLRFSNRPFSLDNFDDYQKHFTVMNYAEGAQLKQVHYDEFIPQFEKQYPGYPWASVQEEVFKAFAELFQLASSRPAPYGICPYPSSRAIYAIDLMLKWSTDAEGQRIMQPQILEVNFSPDCARACKYHPSFYNDVFSTLFLDETAGRPVTQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND99</accession>
    <name>W5ND99_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Rh family, C glycoprotein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as an ammonia transporter. May play a role in the elimination of ammonia in the gill.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND99"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018608"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018640.1">
        <property type="protein sequence ID" value="ENSLOCP00000018608.1"/>
        <property type="gene ID" value="ENSLOCG00000015114.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3796">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182844"/>
    <dbReference type="InParanoid" id="W5ND99"/>
    <dbReference type="OMA" id="YWEMHEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015114">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000001">
        <property type="entry name" value="Ammonium transporter Rh type C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF30">
        <property type="entry name" value="AMMONIUM TRANSPORTER RH TYPE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="13">
        <location>
            <begin position="68"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="490" mass="54087" checksum="597CF27ADA3F0FCF" modified="2014-04-16" version="1">MGILQSLCQSFERPKNTNVRLKLPVVCFIWQTAMIILFGIFIRYNPEADAHWPEFKKQQNITSDIENDFYFRYPSFQDVHVMIFVGFGFLMTFLKRYSFGAVGFNFLIAAFGIQWALLMQGWFHHLDFNDGKIKIGIENLINADFCVAGCLIAYGAVLGKVSAVQLMVMTLFGVTLFAVEEYIILNLIHARDAGGSMVIHVFGAYYGLAISRVLYRPNLDQSKRLQGSVYHSDVFAMIGTLFLWMFWPSFNSAIAGHGDGQHRAAINTYLCLASCVVTAVMISSVVEKKGRLDMVHIQNSTLAGGVAAGTAAEFMLTPYGCLIVGFCCGIISTLGYVYLTPFLESKLKIQDTCGIHNLHAMPGLIGGIVGAITAAAASKEVYSEEGPTQTAHCLRHSQQAPISQIQGGHQAAGIAVALAFGLVGGTIVGIILKLPIWGDPADENCFNDEVYWEVPEDEESLPPPVEYNNHMMVMKQWQNASSWDLMHCEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDE7</accession>
    <name>W5NDE7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Apoptotic protease-activating factor 1</fullName>
            <shortName evidence="9">APAF-1</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Oligomerizes upon binding of cytochrome c and dATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDE7"/>
    <dbReference type="FunCoup" id="W5NDE7">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018656"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018688.1">
        <property type="protein sequence ID" value="ENSLOCP00000018656.1"/>
        <property type="gene ID" value="ENSLOCG00000015153.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157710"/>
    <dbReference type="HOGENOM" id="CLU_005071_1_0_1"/>
    <dbReference type="InParanoid" id="W5NDE7"/>
    <dbReference type="OMA" id="GAYLKWW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015153">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043293">
        <property type="term" value="C:apoptosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000081">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000001">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.370:FF:000001">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000135">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000196">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000502">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017251">
        <property type="entry name" value="Apaf-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041452">
        <property type="entry name" value="APAF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048975">
        <property type="entry name" value="WHD_APAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22845">
        <property type="entry name" value="APOPTOTIC PROTEASE-ACTIVATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22845:SF5">
        <property type="entry name" value="APOPTOTIC PROTEASE-ACTIVATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17908">
        <property type="entry name" value="APAF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21296">
        <property type="entry name" value="WHD_APAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037646">
        <property type="entry name" value="Apop_pept_activating-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CARD" evidence="11">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="655"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="745"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="887"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1052"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1093"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1135"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1261" mass="142084" checksum="A7493F89AB6682EB" modified="2014-04-16" version="1">EVMEEQARSRLLCSRSKLEDDIKALYLMDHMVSDEVLSADEEETVKAKATRREQAAALIDLILRKDNHAYVSLYNALIRESYSDLASLLQKDLPLISPRADKSFPDGESPGVQAVLREGGVPQRPVVFVKRPELVNRIREKLYKLLAAPGWVTVFGMAGFGKSVLAAEAVRDNELLTVCFPGGVHWLAVGQLDKAGLLVKMQSLCFRLDLDSQFCQRPPSTIEEAKDRLRFLMIRKYPRSLLILDDVWDGAVLRAFDIQCRVLLTTRDRSLTDCVAGQKFEVSVGRGLEEDKALEVLAVFVSKKVAALPEQARQIVQECKGSPLVVSLIGALLREFPDRWAYYLRQLQNKQFKRIRKSTSYDYEALDQAMSASIEVLPDDHKDFYKDLTVLEKDVKVPVKVLSVLWDLEPEEVEDILQEFVNKSLLFRDSNKTPYLYYLHDLQLDFLTEQNRYRIQELHSKVVHQYQKYYKNAHPTAGDEDCLYWYRYLGYHMARGNLHEELYSFLFSLDWVKTKAQIMGPASLINDYVEYSSILDKENSTVRENFQEFLSLNGHLLEQRPFPDVVQLGLCQPSSSEVYRQARLKAEEEARKGAFYLDWINKSSLESLSRLIVQPHKGSIYYACFSRDGEKIASCGADRTVQVFRSVTGEKVLDIVAHEEEVLCCAFSPDDKLIATCSTDRKIKIWNAERGKLIRIYEEHSEQVNHCQFTNSSYRILLATGSNDCFLKLILKLWNINKPTCQNTLFGHTEAVNHCCFSPDDAHLASCSSDGTLKLWQVSSGNEWKSIDVFSYFPSSPDSREEGDVIVKCGAWSPDGSRIIAAAKNSVFVFDADTCDLLSEIKTSRHGTIQFCDFCPSGQLVAIALSHYTVEKTQLWEIESNKKVADCSGHLSWVHCVKFSPDGSLLLSSSDDQTVRLWETKKVHTSSAVSLKRDSDVLFDHNEISVLAPDSRNRLQLRSGNTGEVVYQSEEQESRIRSSCLCKEPAVSALGRDDGVVKVLDIPNGNTVSTLRGHTKPVQHCEFTADGRTLITCSEDTTIRVWDWRSSECKVLKGHTEQVKKCVLLDSSRLLSWSFDGTVKVWNMATGELIQDIFCHKGAILSCDVSPDGKKFSSASADKTAKVWSFASSGLLHTLTGHKACVRSVRFSGDGACLATGDDHGEIRIWGVVDGALLQICTRGDKDSMESLHGGWVTDLHFSPDGEMLVSTGGYIKWWDVKTGRALQTFYTTGTNLKGIHVSPDFRTFVTIDNIGILYVLKKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDJ8</accession>
    <name>W5NDJ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycerophosphodiester phosphodiesterase domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDJ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018707"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018739.1">
        <property type="protein sequence ID" value="ENSLOCP00000018707.1"/>
        <property type="gene ID" value="ENSLOCG00000015200.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107077912"/>
    <dbReference type="CTD" id="54857"/>
    <dbReference type="eggNOG" id="KOG2258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159625"/>
    <dbReference type="HOGENOM" id="CLU_024259_3_0_1"/>
    <dbReference type="InParanoid" id="W5NDJ8"/>
    <dbReference type="OMA" id="DPPGCCS"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015200">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOINOSITOL INOSITOLPHOSPHODIESTERASE GDPD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="10">
        <location>
            <begin position="254"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="568" mass="64377" checksum="56114F31AE02C566" modified="2014-04-16" version="1">MTWTGGVRSQPAECAGGAEGDTEGGRHKTGGAESGSMPQDSFCRKCLRGVYSCRWRSGSQNKHRCSCCWFFFLALACLLKAGWIYVCWAAINDRDDVNWKAFSLLRFRWVNWFIVVVIMSSVLVSYSCLLLVFALFQVALREPLNLHCTHKVLLLLAVLIIAAGVAGICLEWKEEWLTVFMSLQMTGPFLQIGGVVSVSLLGWFVFQRFFMARRAVSRVVILLAYLVVGAAVFLSPLLISSPCFLEKHNLPPKPALIGHRGAPTMSPENTMLSFRRSLECDVKAFETDVIISQNGIPFLMHDDKSLRRTTDVSEKFPGRVENASHTFTWEELKTLNAGDWFLKSDPFHTVSSLSEEDKAAIRNQTIPALTELLDLAKQHNTSIIFDLKGDSDELNVTLRTVQAILDSNIPQERVLWLPSRYRKLVKMKAPDFKHVYANKSLMEDDGGDQLNLKYSSLTPAEIRDLRRGNVSVNLYVVNESWLFSLLWCSGVTSVTTNTCRHFKNMTQPAWILAPDTYTMLWITVDSVFLLVILGLFLLQRRRFTRGTENEEHRNGYSVWNEQELDTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDY1</accession>
    <name>W5NDY1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centromere protein I</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-I/CTF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDY1"/>
    <dbReference type="FunCoup" id="W5NDY1">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018840"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018872.1">
        <property type="protein sequence ID" value="ENSLOCP00000018840.1"/>
        <property type="gene ID" value="ENSLOCG00000015310.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013235"/>
    <dbReference type="InParanoid" id="W5NDY1"/>
    <dbReference type="OMA" id="RVFKNYY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015310">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000939">
        <property type="term" value="C:inner kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22647">
        <property type="entry name" value="CTF3_NTD_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012485">
        <property type="entry name" value="CENP-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208:SF2">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07778">
        <property type="entry name" value="CENP-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="702" mass="80430" checksum="3CBC671AF4434AAD" modified="2014-04-16" version="1">RRREAEDPLQLALLYFCQVEAGTPWKGNDVLEKHFEAVERVAFSRGLSPEGIEILLDFALGLRMGSKCGIRVLKCLIPASVIPESAVVKGVSWFCVGKMPPNMQIMFIRWFITMFDLIESKERLHSLYGFFFCFLSDENLCPYICHLLYLLTRRESEYSLVKPYRLRKLMNLQSKMGEQPFLMVLLSLYKVFCPEMVSLTIPARIKNGFKNYDSTWKNALSAVQKRNKAEIVMELQAAVSVRPQSASRKRKRNCHLSVPPLGSCAPPPGAREDGVLGSRVFPLEQLRTFPQLLQNIHLLELPAQMGSVLNSPLVLHYINCITDDSVFLRLDYWLGHALHEEFLFCKDTSYENNSEAIEFFSSLITAQQFLQEGFLSTEVFTLKFLRVWDGSLFRSQILSLLSYIPVIPCSDVKQLLFEPLIQLFFSCSLYFKCSVIECLNSMLVKWLTWHAVFASEDQFDMSISGHSPTNMSLSGIMSSVVELVEFVGRISTIALHMEGYNTLLLHFVLNFYETVCDMYVKYDFPLAIMPPAGVFYPALLSKDPVSVNQLCHIMFRYRVNLLSAKDPEKQGKKTFLVNSKTYRKYNQYVVAMVWCLWSSKAFMAGMEIEINPDLLAQAGVAEYRSTFNIVHHPALTGYAIDFFHKCWPESKQLNLSAIKQGRHWDWYLEYLFTQGFDGLREFIESSIRRLSGPAGLNSSRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFV9</accession>
    <name>W5NFV9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Probable proton-coupled zinc antiporter SLC30A3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Proton-coupled zinc antiporter SLC30A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">Solute carrier family 30 member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Solute carrier family 30 member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Zinc transporter 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Zinc transporter 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Electroneutral proton-coupled antiporter concentrating zinc ions into a variety of intracellular organelles including endosomes, zymogen granules and mitochondria. Thereby, plays a crucial role in cellular zinc homeostasis to confer upon cells protection against its potential cytotoxicity. Regulates the zinc concentration of milk, through the transport of zinc ions into secretory vesicles of mammary cells. By concentrating zinc ions into lysosomes participates to lysosomal-mediated cell death during early mammary gland involution.</text>
    </comment>
    <comment type="function">
        <text evidence="24">Probable proton-coupled zinc ion antiporter mediating the import of zinc from cytoplasm into synaptic vesicles and participating to cellular zinc ion homeostasis in the brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="31">Homodimer. Interacts (via lysosomal targeting motif) with AP3D1; in AP-3-mediated transport to lysosomes. Interacts with TMEM163.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
            <location evidence="4">Synaptic vesicle membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Synapse</location>
            <location evidence="23">Synaptosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Zymogen granule membrane</location>
            <topology evidence="30">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625738.2">
        <property type="nucleotide sequence ID" value="XM_006625675.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199414.1">
        <property type="nucleotide sequence ID" value="XM_015343928.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFV9"/>
    <dbReference type="FunCoup" id="W5NFV9">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019518"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019550.1">
        <property type="protein sequence ID" value="ENSLOCP00000019518.1"/>
        <property type="gene ID" value="ENSLOCG00000015851.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698596"/>
    <dbReference type="KEGG" id="loc:102698596"/>
    <dbReference type="CTD" id="7780"/>
    <dbReference type="eggNOG" id="KOG1482">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156072"/>
    <dbReference type="HOGENOM" id="CLU_013430_0_1_1"/>
    <dbReference type="InParanoid" id="W5NFV9"/>
    <dbReference type="OMA" id="VNIMAYI"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015851">
        <property type="expression patterns" value="Expressed in embryo and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042589">
        <property type="term" value="C:zymogen granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062111">
        <property type="term" value="P:zinc ion import into organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000011">
        <property type="entry name" value="zinc transporter 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF30">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0050">Antiport</keyword>
    <keyword evidence="22" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="21" id="KW-0458">Lysosome</keyword>
    <keyword evidence="20 35" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10 35" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 35" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="36">
        <location>
            <begin position="80"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="37">
        <location>
            <begin position="310"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00037129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00040652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00042040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00042216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00053997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00060459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00061875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00070392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00076850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00077285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="400" mass="43911" checksum="E19406090381C55C" modified="2014-04-16" version="1">MDTAVGSEKSHLINEKNTKMYTLKLKSPFPGSKEGYPNFPFENGDTATAIELKRPVSVHCHGNRAVCGESHEKQLARKKLYIASLVCLIFMIGEVIGGYLAHSLAIMTDAAHLLTDFGSMMVSLFSLWISSRPPTKIMSFGWHRSEILGAFISVLSIWIVTGVLVYLAVERIVNNNYEIDGHVMLITSGCAVGVNVIMALILHQSSTLHSHSHGSGYHKIDEGGESPISHGHSHSLLGAHGNTSVRAAFIHVIGDLLQSVGVLVAAIIIYFRPEYKIADPICTFLFSVFVLGTTTTILRDVFRILMEGAPKGIEFNSVKDVLLSLKAVKAMHSLHLWALTLGQTLLSVHIAIEENCDPQSVLKEATELLQTKFGFYSTTIQVEHYSEDMAHCIQCQDPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGP6</accession>
    <name>W5NGP6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF4E-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625857.1">
        <property type="nucleotide sequence ID" value="XM_006625794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGP6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019805"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019838.1">
        <property type="protein sequence ID" value="ENSLOCP00000019805.1"/>
        <property type="gene ID" value="ENSLOCG00000016072.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689159"/>
    <dbReference type="KEGG" id="loc:102689159"/>
    <dbReference type="eggNOG" id="ENOG502S4SY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159932"/>
    <dbReference type="HOGENOM" id="CLU_111706_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGP6"/>
    <dbReference type="OMA" id="VNDADHM"/>
    <dbReference type="OrthoDB" id="19729at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016072">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008606">
        <property type="entry name" value="EIF4EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669:SF14">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05456">
        <property type="entry name" value="eIF_4EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="12944" checksum="4FECE930FD99C77B" modified="2014-04-16" version="1">MSSGCQKTTSRAIPTRRVIVNDATQMPIDYSTTPGGTLFSTTPGGTKIIYDRKFLMECRNSPVARTPPRFLPNIPGVTSPNREEDKPGTNHTEPNHVATNSNHLDKPSGDDAQFEMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGT6</accession>
    <name>W5NGT6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CTCK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGT6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019845"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019878.1">
        <property type="protein sequence ID" value="ENSLOCP00000019845.1"/>
        <property type="gene ID" value="ENSLOCG00000016106.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171304"/>
    <dbReference type="HOGENOM" id="CLU_057140_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGT6"/>
    <dbReference type="OMA" id="CEKEIIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016106">
        <property type="expression patterns" value="Expressed in intestine and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47246">
        <property type="entry name" value="MUCIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47246:SF1">
        <property type="entry name" value="MUCIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="VWFC" evidence="7">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="6">
        <location>
            <begin position="144"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="162"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26020" checksum="8F17827894FC0A64" modified="2014-04-16" version="1">MLHAEKHKDICVSDCSNCKGPLGEPKKTGETWETNCHICTCSNITNTEECTPKEIKSPPACKDNEILVTYNATGCCQMQYCVEKTCEYGGVKYKVGDEWKDYSKPCNSYVCQKSGITTKTKMCPAQNCPEDQRILDSDKCCYMCNETCYPKKSTVDVTVDNCIGQVEMTSCEGHCESQPQLESVGGIFRMKQTCHCCLETSIQTRNATLNCDLNTPKLYSYKYITGCKCLACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH34</accession>
    <name>W5NH34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein Rap-1b</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Probable GTP-binding protein that possesses GTPase activity. May play a role in endothelial cell polarity and endothelial barrier function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01008861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208343.1">
        <property type="nucleotide sequence ID" value="XM_015352857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH34"/>
    <dbReference type="FunCoup" id="W5NH34">
        <property type="interactions" value="1496"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019943"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019976.1">
        <property type="protein sequence ID" value="ENSLOCP00000019943.1"/>
        <property type="gene ID" value="ENSLOCG00000016180.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697231"/>
    <dbReference type="KEGG" id="loc:102697231"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154429"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="W5NH34"/>
    <dbReference type="OMA" id="AAQFNCA"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016180">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071320">
        <property type="term" value="P:cellular response to cAMP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000301">
        <property type="term" value="P:negative regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032486">
        <property type="term" value="P:Rap protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04175">
        <property type="entry name" value="Rap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000182">
        <property type="entry name" value="ras-related protein Rap-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038851">
        <property type="entry name" value="Rap1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="185" mass="20914" checksum="1F5F649D2FD254DF" modified="2014-04-16" version="1">MMREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLVRQINRKTPAPGKARKKSNCQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI77</accession>
    <name>W5NI77_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metalloproteinase inhibitor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tissue inhibitor of metalloproteinases 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI77"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020336"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020370.1">
        <property type="protein sequence ID" value="ENSLOCP00000020336.1"/>
        <property type="gene ID" value="ENSLOCG00000016462.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159601"/>
    <dbReference type="HOGENOM" id="CLU_084029_0_0_1"/>
    <dbReference type="InParanoid" id="W5NI77"/>
    <dbReference type="OMA" id="GCRIKPC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016462">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="NTR_TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.370.10:FF:000001">
        <property type="entry name" value="Metalloproteinase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000005">
        <property type="entry name" value="Metalloproteinase inhibitor 3 precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF22">
        <property type="entry name" value="METALLOPROTEINASE INHIBITOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="6" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
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    <feature type="chain" id="PRO_5004867796" description="Metalloproteinase inhibitor 3" evidence="13">
        <location>
            <begin position="32"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="14">
        <location>
            <begin position="32"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="34"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="44"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="153"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030101"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="219" mass="25268" checksum="3DEEB5A527BD82FD" modified="2014-04-16" version="1">QTSERMSLLVCPLLSVLFVLSSLLMNPFTEACSCALSHPQDAYCNSDIVIRAKVVGKKLLKDGPFGTMRYTVKQMKMYKGFNKVQHVQYIYTDASESLCGVKFDVNKYQYLITGRVFDGKVYTGLCNFNEKWERLTLSQKKGINHRYQLGCNCRIKPCHYLPCFVTSKNECLWTDMLSYYGYPGYQSRHYACIQQKEGYCSWYRGMSSRDKTIINATDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIF3</accession>
    <name>W5NIF3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclear receptor coactivator 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIF3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020447.1">
        <property type="protein sequence ID" value="ENSLOCP00000020412.1"/>
        <property type="gene ID" value="ENSLOCG00000016524.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683089"/>
    <dbReference type="eggNOG" id="KOG2372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155141"/>
    <dbReference type="HOGENOM" id="CLU_007095_2_0_1"/>
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    <dbReference type="OMA" id="SDTQAAF"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
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        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016524">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:response to oxidative stress"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LysM"/>
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        <property type="entry name" value="LysM_dom_sf"/>
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    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF68">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="4">
        <location>
            <begin position="716"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="435"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="877" mass="98427" checksum="74D5B22F352650AD" modified="2014-04-16" version="1">MEKKERKPSYFARLKKRKQIKQNSERTADKSSEGLQPQTGQVKQDKTSISEAGLSLPSDLKKSTKENTSDEDKKKKNCQRKEIRRSDLKKNCGVDDNRNKTRTKKEKRRVIQKPSGTVEYTAGPKDTLNCIALKFNITPNRLVEINRLFSHTIVPGQTLFVPDVTQQPTETNSLSVSPCSPASAFSSDDPEFDKMSDADSARKASKPLHRILSSASEEEEPSTVKFLKMSCRYITDGKGVVGGVMIVTPNNIMFDPHKSDPLVIENGCEEYGLICPMEEVVSIALYNDISHMKIKDALPSDLPKDLCPLYRPGEWEDLSSEQDINPFSRFKAMSKEKCLASVDEAVYVKHESETTEKSLSDEGFTEIEPLVTGNTDESLIEPLANGICTGSSDDTQIKKGTLEALVHQSSADKELVAHKEHTLYPVVSMGLEEREDPELEKVGSNQNAQETPVYIHTEIPKGKALKEGKLPDDGRGGETAHATAEPSYVDSAESETPNDTCLVTSVQETTMDQNRMLSSCERAEVSAKKNQPGPDQSTDALNGDGPLSVPQSEEVEQQNRESEIKSWLFKRIQGPIEDMLPSKEEKSKKPPMFLCIKVGRPMRKTFACQSASPAHQYGRRGKQPEYWFAVPQERVDHLYAFFVQWSPDIYGNDAKEQGFIVVEKDELDMIDNFFGDPVPKSWEIITVNEAKRRQSYCSYEDEDEEDLLPALNDQSVLLEDTHIEKLAQCLPARTQGYPWRLVYSTAVHGTSLKTLYRNLAHLDSPVLLVIKDVDNQVFGALTSHPLKVSDHCYGTGETFLYTFSPEFKIFRWSGENSYFIKGDTDSVELGGGGGHFGLWLDADLYHGSSYPCYTFQNETLSKKEDFIVQDLEVWTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKK8</accession>
    <name>W5NKK8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKK8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021167"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021203.1">
        <property type="protein sequence ID" value="ENSLOCP00000021167.1"/>
        <property type="gene ID" value="ENSLOCG00000017128.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170074"/>
    <dbReference type="HOGENOM" id="CLU_000680_32_6_1"/>
    <dbReference type="InParanoid" id="W5NKK8"/>
    <dbReference type="OMA" id="IRCISEK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="176"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="47919" checksum="BC00C4C8E36E7FC2" modified="2014-04-16" version="1">VAIRERNEARKKMLQRPTRTSTAEFSRKRTLAKRLCRYRKREWQNRMLQETEELYRNKETRKVYQKITENKNGFQPRLAMCVDKEGNLIGDGRKILERWAEYFEELLNDAGEMDETGEEEEADEDTTERESCWNPPTVFEFKEAIRNMKNNKAPEEDAITAEMLKYDEEELADTSYKIISIILGEKWSITLVCPIHKKGSKLDCLNYCRISLLNVAYKVFPKVLASRLEAHAEQVLGHTQCGFRCNQSTTDHIFTIRCISEKCYEYNIDVHKLFIDNKRAYDSVDRAYLYQTLGQLGVAWQLIRLVKMTMTKVKVQNQESHEFQVRQGLRQGHALSSLLVNLVLESVKRNIDINKGGTLLNRGPYLAYVDDIDLLGRNIWTLKENFIQLEEALTRTGLTINMAKIKYMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKX5</accession>
    <name>W5NKX5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EARP complex and GARP complex interacting protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat EIPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032618">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKX5"/>
    <dbReference type="FunCoup" id="W5NKX5">
        <property type="interactions" value="1067"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021284"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021320.1">
        <property type="protein sequence ID" value="ENSLOCP00000021284.1"/>
        <property type="gene ID" value="ENSLOCG00000017215.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1007">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111137"/>
    <dbReference type="HOGENOM" id="CLU_050772_0_0_1"/>
    <dbReference type="InParanoid" id="W5NKX5"/>
    <dbReference type="OMA" id="HQFLALH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017215">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060341">
        <property type="term" value="P:regulation of cellular localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000156">
        <property type="entry name" value="protein TSSC1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059104">
        <property type="entry name" value="Beta-prop_EIPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040323">
        <property type="entry name" value="EIPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14205:SF15">
        <property type="entry name" value="EARP AND GARP COMPLEX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14205">
        <property type="entry name" value="WD-REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23609">
        <property type="entry name" value="Beta-prop_EIPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EIPR1-like beta-propeller" evidence="6">
        <location>
            <begin position="7"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="225"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="388" mass="43823" checksum="A929380AC4172A1E" modified="2014-04-16" version="1">MEDDAPVIYGLEFQARALTAQTAETDAIRFLVGTQSLKFDNQQIHIIDFDDENNIINKNVLLHQVGEIWHISTSPADKSVLSTCYNKTSDSKVVTCAAVWRMPKELESGSHESPDDSSSNPQTLELLCHLDNTAHGNMACVLWEPMGDGKRLISLADNHLLLWDLQENCTKATISSSATLEGKGQLKFTSGRWSPHHNCTQVATANDTAIRGWDIRSMSQMYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDVRNISEPVKSLEEHSHWVWSVRYNHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDEDDLSDPEDNHQEEKSKEPLQDSIIATYEEHEDSVYAVEWSSADPWLFASLSYDGRLVINRVPRALKYRILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLP5</accession>
    <name>W5NLP5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLP5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021554"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021591.1">
        <property type="protein sequence ID" value="ENSLOCP00000021554.1"/>
        <property type="gene ID" value="ENSLOCG00000017449.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RDRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183023"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NLP5"/>
    <dbReference type="OMA" id="FCEFTIL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF860">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="46"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="36644" checksum="0FB021E8F7476633" modified="2014-04-16" version="1">FIFKLLETMNSSDVTVFTLHGLNDTRTNKSIFFTFTVLFYLFIIFFNLILIAAVLLEKSLHEPMYIFICNLCINALYGSAGLYPKLLNDFLSDTHVISYTACITQLFVIYSFVFCEFTILTMMAFDRYIAICRPLEYHAVMTPLTVVKFLMFSWLFPLFEMMIAVLLTLRLPLCGSHIDKFYCENWSVVKLSCVDTTLNNLYGYILILKYTVQSLFVIFSYILIIRTCLMSNESRKKFMQTCLPHLITLISFLVTMLFDVMYTRYGSRNTPQILRNIMAVEFLVIPPLLNPLIYGLNLTQIRRTVFKLCNRKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLT4</accession>
    <name>W5NLT4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Lysophosphatidic acid receptor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLT4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021630.1">
        <property type="protein sequence ID" value="ENSLOCP00000021593.1"/>
        <property type="gene ID" value="ENSLOCG00000017488.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV8W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NLT4"/>
    <dbReference type="OMA" id="TINSRHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017488">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15155">
        <property type="entry name" value="7tmA_LPAR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF41">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01067">
        <property type="entry name" value="P2Y5ORPHANR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="50"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="316"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="364" mass="40483" checksum="E62D1E2C106CAFAC" modified="2014-04-16" version="1">DHLTDPPVMASLGLNESGGDRPEDCAIDDSFKYNLYGAVYSVVFVLGLITNCASLFVFCFRMKLRNETTLFMTNLAVSDLLFVFTLPFKIFYNLNRHWPFGDGLCKVSGTAFLTNIYGSMLFLTCISMDRFLAIVYPFRSRAVRTRRNAGIMCAGVWLLVLGGGISVSFFSTTNVSNSSTTCFEGFSKKTWRTYLSKITIFIELVGFLIPLLLNLGCSSMVLRTLRKPATLCQIGTNKERVLRMIVVHVAIFVVCFVPYNSVLFLYAMVRSQALASCWLERFARTLYPITLCIATLNCCFDPFVYYFTSESFQKSLSTGKTPAKNDGTLKSEQPLSTKGSPPSTTKGGSGDEHRTASNGRELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM86</accession>
    <name>W5NM86_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NM86"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021745"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021782.1">
        <property type="protein sequence ID" value="ENSLOCP00000021745.1"/>
        <property type="gene ID" value="ENSLOCG00000017640.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_045922_2_0_1"/>
    <dbReference type="InParanoid" id="W5NM86"/>
    <dbReference type="OMA" id="NIRCLNC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
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        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="173" mass="19921" checksum="1F8B5F6470A1F0E5" modified="2014-04-16" version="1">SEVCVKHRDEEFMKEFELLGLASDDTRWVIVNMYDPFLSDDDITAFLEMHVESASAPKRIYDRFGYWTGRRQYEVTLKVNKNYEGGFAHPPALFALGGSRGYLFYRDQPPFCRKCHEIGHVEKDCRTTSCTRCGAEGHTKRTCRIKVCSLCGADTHLYRACLKRDLTFVAAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMD3</accession>
    <name>W5NMD3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized LOC107075466</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMD3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021829.1">
        <property type="protein sequence ID" value="ENSLOCP00000021792.1"/>
        <property type="gene ID" value="ENSLOCG00000017687.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107075466"/>
    <dbReference type="eggNOG" id="ENOG502R546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008158"/>
    <dbReference type="HOGENOM" id="CLU_703924_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMD3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017687">
        <property type="expression patterns" value="Expressed in zone of skin and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039051">
        <property type="entry name" value="SE-CTX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472:SF10">
        <property type="entry name" value="RAPUNZEL 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472">
        <property type="entry name" value="RICIN B-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="383" mass="44537" checksum="0388E6ED517A2121" modified="2014-04-16" version="1">MEFLLQNRKCVDVGTDVLDILARKAAELHPALKLLTFISRQILDCPESTEAQYFIGQLSKLNEKIGSIQTDFKDLQSEIEKSFMNQQYYNDEITILHHYDLFLKLMKTYTADKKDESDKIHFFDQVDGTDVTIMELYEGVMGSNNQRPILDIIRDNKKRSLQSVEQFCIHLKKLFVMELVVLCTHSKLKGNGKEEKLMEEWGEKLKDVNRKIKEVIDDCTDNFAVQARSDMEGLLQGGGADKDDEELPKQLLSFLEKKYNWVSWSVRIFNPSESRIWKLLAGNKYTSISGCNHFSVELQNIQLVVSYCQHPKPIPKDDIEKLVETMGWRRNTAGVVKTLHRRFPRYLAHVVSTVKNVKEANNFKDDHYYFKKHNVAYICIHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMP6</accession>
    <name>W5NMP6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TLR adaptor interacting with endolysosomal SLC15A4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639334.1">
        <property type="nucleotide sequence ID" value="XM_006639271.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219535.1">
        <property type="nucleotide sequence ID" value="XM_015364049.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219536.1">
        <property type="nucleotide sequence ID" value="XM_015364050.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219537.1">
        <property type="nucleotide sequence ID" value="XM_015364051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMP6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021905"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021943.1">
        <property type="protein sequence ID" value="ENSLOCP00000021905.1"/>
        <property type="gene ID" value="ENSLOCG00000017801.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685624"/>
    <dbReference type="KEGG" id="loc:102685624"/>
    <dbReference type="eggNOG" id="ENOG502QTA8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011425"/>
    <dbReference type="HOGENOM" id="CLU_081014_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMP6"/>
    <dbReference type="OMA" id="MFHDSSP"/>
    <dbReference type="OrthoDB" id="9892060at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017801">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035751">
        <property type="term" value="P:regulation of lysosomal lumen pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034121">
        <property type="term" value="P:regulation of toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027869">
        <property type="entry name" value="TASL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14889">
        <property type="entry name" value="RCG36411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14889:SF3">
        <property type="entry name" value="TLR ADAPTER INTERACTING WITH SLC15A4 ON THE LYSOSOME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15133">
        <property type="entry name" value="TASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="33286" checksum="F3BDF6E72287B333" modified="2014-04-16" version="1">MLCESFLWAIPYGNDGDRDNQMPSKFEEVENYALSSVTSAINLTSNSPETELPNKPSKSKSMGRFIKKESSWNKSQSKKSEVQQCSRQISPVVHIPRQVTSSKEAYLVPSSCKSICKDYNDLQIAGDQVMPLSSSTVSFTLDSSVEFAEGPFLQSCEIPPAMETPRPSLEYVRKPIKADSSCWRVESVKEKSFLHHSRPLSNAMLNEYLEQKIMDLYKQYMVDSMLTSNSPTTILASELILTNVDQITQQISREQNMEAAKAKDMVISCLLRVASGIQSSELSTPQLQISSERSINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMQ6</accession>
    <name>W5NMQ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Suppressor of cytokine signaling 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Regulates KIT degradation by ubiquitination of the tyrosine-phosphorylated receptor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209163.1">
        <property type="nucleotide sequence ID" value="XM_015353677.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209165.1">
        <property type="nucleotide sequence ID" value="XM_015353679.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209166.1">
        <property type="nucleotide sequence ID" value="XM_015353680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMQ6"/>
    <dbReference type="FunCoup" id="W5NMQ6">
        <property type="interactions" value="903"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021915"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021953.1">
        <property type="protein sequence ID" value="ENSLOCP00000021915.1"/>
        <property type="gene ID" value="ENSLOCG00000017811.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686146"/>
    <dbReference type="KEGG" id="loc:102686146"/>
    <dbReference type="CTD" id="558703"/>
    <dbReference type="eggNOG" id="KOG4566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154847"/>
    <dbReference type="HOGENOM" id="CLU_038160_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMQ6"/>
    <dbReference type="OMA" id="PRVNHND"/>
    <dbReference type="OrthoDB" id="6270897at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017811">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="term" value="P:regulation of growth"/>
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        <property type="entry name" value="SH2_SOCS6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
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        <property type="entry name" value="Suppressor of cytokine signaling 6"/>
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        <property type="entry name" value="SH2 domain"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2"/>
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        <property type="entry name" value="SH2_dom_sf"/>
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        <property type="entry name" value="SOCS6_SH2"/>
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        <property type="entry name" value="SOCS6_SOCS"/>
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        <property type="entry name" value="SOCS_box-like_dom_sf"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF28">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SOCS"/>
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        <property type="entry name" value="SH2 domain"/>
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        <property type="entry name" value="SOCS box-like"/>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="379"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="11">
        <location>
            <begin position="481"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="530" mass="58949" checksum="C5BBE80A83618F6E" modified="2014-04-16" version="1">MKKISLKTIRKSFNLNKGKEEGDFVVVQQPALTPDFVKEESIFGGCYSKELASCDLNGDEEKGHKSRSKSESLMGTLKRRLSAKQKPKGKGGSPAVNSADDDDTFSSSSAPVGFNDVKAQRPLRSTSLRSHHYSPTPWPLRPAASEETCIKMEVKVKALVHSSSPSPALNGVRKEFHDLQMDGLFQEQSSSLKNSEAQNGDLHLNIDEHVPVVIGLTPQDYIQYTMPLDEGMYPVEGSRSFCLDSSSPMEVVSQMEGSALHVDEGQVDQDLVMSPDIFIDPSVNGLLIGTTGVVLQNPRVDAPPLSPLLPPLPSSQIQRNFPGLSGADAQVAERVRHHLNFDPNSAPGVGRVYDSVQNSGPMVVTSLTEELKKLAKQGWYWGPITRWEAEEKLANLPDGSFLVRDSSDDRYLLSLSFRSQGKTLHTRIEHSNGRFSFYEQPDVEGHTSIVDLIEHSIKDSENGAFCYSRSRLPGSATYPVRLTNPASRFMQVRSLQYLCRFVIRQYTRIDLIQKLPLPNKMKDYLQEKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMX0</accession>
    <name>W5NMX0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ras homolog family member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMX0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021979"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022018.1">
        <property type="protein sequence ID" value="ENSLOCP00000021979.1"/>
        <property type="gene ID" value="ENSLOCG00000017876.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102682621"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163656"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="W5NMX0"/>
    <dbReference type="OMA" id="THVALIC"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017876">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="196" mass="22050" checksum="14C4E2E263935F4B" modified="2014-04-16" version="1">MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRNDENVRNELSRMKQEPVKTEDGRAMAVRIGAYDYLECSAKTKEGVREVFETATRAALQKRSRPSGGCLNCCKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNA8</accession>
    <name>W5NNA8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNA8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022158.1">
        <property type="protein sequence ID" value="ENSLOCP00000022117.1"/>
        <property type="gene ID" value="ENSLOCG00000018016.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2533333_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNA8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018016">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="84" mass="8290" checksum="3153F91C3E180F19" modified="2014-04-16" version="1">GAWGGGSRGGGSEVVPGLVLGLGGCEVILQEGLQVLEGGALLGVLLPALHHELVQGAGAVLRAGHPVATLHLLQHLPVVHACAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNV8</accession>
    <name>W5NNV8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuropeptide Y receptor type 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">NPY-Y5 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNV8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022317"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022358.1">
        <property type="protein sequence ID" value="ENSLOCP00000022317.1"/>
        <property type="gene ID" value="ENSLOCG00000018216.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161766"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_1_1"/>
    <dbReference type="InParanoid" id="W5NNV8"/>
    <dbReference type="OMA" id="RHHQDTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018216">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000393">
        <property type="entry name" value="NPY5_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF10">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01016">
        <property type="entry name" value="NRPEPTIDEY5R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="55"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="440" mass="49942" checksum="5C3D0E5A0EA8C077" modified="2014-04-16" version="1">MDLEYPNGSEVSKDNTSAFFNSDFPLWEDFNSSVDDVRYFLIGLYTFISILGLLGNILILTALTRKWKEKTIINFLVGNLAFSDILVVLFCAPFTLTCVLLDHWVFGDVMCHIVPFLQCVSVMVSTLVLMSIAIVRYHMISHPLSRHLSLNAGYILLGIIWTLALSICSPLPVFHKTVDLSETFHLESLKNKSLCIESWPSGGYRTAFTISLLFVQYILPLVCLTVSHASVCRRVSSSVPSHLQVQTEEKEMINLTLQQPNQKSCIPTQFTDSEKWKLVLAQKQRKRYNKKTSSVVPAMYSHENDSAEYLHPQSQHHPSTLLLSGVPVCFELKEEEATELHKMLTISKSISRMKKRSKSVFFKLTIVVLAFAISWMPLHIFHLVTDFSANHISSRHFKLIYCICHLLGMLSCCLNPVLYGFLNNGIKSDLLSLLKCFYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP68</accession>
    <name>W5NP68_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TLR4 interactor with leucine-rich repeats</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP68"/>
    <dbReference type="FunCoup" id="W5NP68">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022427"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022468.1">
        <property type="protein sequence ID" value="ENSLOCP00000022427.1"/>
        <property type="gene ID" value="ENSLOCG00000018326.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695799"/>
    <dbReference type="KEGG" id="loc:102695799"/>
    <dbReference type="CTD" id="9865"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161975"/>
    <dbReference type="HOGENOM" id="CLU_357128_0_0_1"/>
    <dbReference type="InParanoid" id="W5NP68"/>
    <dbReference type="OMA" id="TDPCDFN"/>
    <dbReference type="OrthoDB" id="2013775at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018326">
        <property type="expression patterns" value="Expressed in embryo and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000169">
        <property type="entry name" value="TLR4 interactor with leucine rich repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868021" evidence="7">
        <location>
            <begin position="22"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="658"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="552"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="764" mass="85916" checksum="C51309E39A438EA0" modified="2014-04-16" version="1">MANLCAIYLIILLPFIYSANAICPDRCDCQNSQHLLCTNRGLRSVPKAAIQNPEEILIFSLGGNFINNISAFDFVRFSQMIRLDLQYNQLQSIHPKAFEKLSRLEELYLGNNLILSVSPGTLNSLKKLRILYANNNEIRKLTPDYFANLDNLIKLRVDGNSIEVLQDALFKSLTNLLYLHLESNKIRYIHRNAFTKLGKLRFLNLSGNKQTALRSVLTFAHLSSLTTLLLSENDIQHVGDGVFQNLKKLSKLSLSNNKISHLATQALRGLSAVRELLIDGNLLTDIPLGLLDPLEKIEELDFSHNLISEVNSLAFRESKHLKVLKLKNNRLTALSGNIFAFNNGLYSLDLNENNWTCDCRLGGFKRWMNSAHSQGKLLTVFVQCRHPLLLKGKYLDYLNSSQLLSSGNDSKVCLFSAADSLEDRTPGAPGEEGSLPRGEANIQADQGGPGSPEKLLSEKNKRKKEASAHRTRPPASARRNGSEVAGKLEQNKSLGTPANRNFTVLAEGGSLDPLSLTTPQPYEGKVEKLNLFQQENGAFPLVTDPCEFNRFFILNITAEQVSSTTASVRWKVLEHRGPKNSPVHFRVLFDRFGQPVRFHRFIYVRDQSNSVTLQELKEDTTYMVCIESVIADLVCQVASRDHCTGVVTLPEKLTAVDFQLLTVIMLGVNALLVFLVLSVWFSKFLRKRLNRRKSAVHVRQMYSTRRPLRSMGTGVSSDFTGYQASRPRNVMCAIDEADLIEFPCDRFLDNGVRRDEVIQQRFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPA4</accession>
    <name>W5NPA4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Basic helix-loop-helix family, member e23</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01035849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220451.1">
        <property type="nucleotide sequence ID" value="XM_015364965.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPA4"/>
    <dbReference type="FunCoup" id="W5NPA4">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022463"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022504.1">
        <property type="protein sequence ID" value="ENSLOCP00000022463.1"/>
        <property type="gene ID" value="ENSLOCG00000018362.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685212"/>
    <dbReference type="KEGG" id="loc:102685212"/>
    <dbReference type="eggNOG" id="KOG3898">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162992"/>
    <dbReference type="HOGENOM" id="CLU_070971_2_0_1"/>
    <dbReference type="InParanoid" id="W5NPA4"/>
    <dbReference type="OMA" id="NCPEKCT"/>
    <dbReference type="OrthoDB" id="10011855at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018362">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18953">
        <property type="entry name" value="bHLH_TS_bHLHe23_bHLHb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000026">
        <property type="entry name" value="Basic helix-loop-helix family, member e23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF53">
        <property type="entry name" value="CLASS E BASIC HELIX-LOOP-HELIX PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="114"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="237" mass="25786" checksum="C457BDDDAF74ACF4" modified="2014-04-16" version="1">MNVGEENIFHGSDKRMNDLKSIGNETLIDLTQRYGQAAFSFGAGHGAGSPGRYPMSGASDFLSGQTAKSTESGGEQTSDDDDSFDHLDPRKRSSAFDEDKHSSSLTKKPKEQRSLRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQTLTSPIPAALAPFGQAAVYPFSGTALATCAEKCTSYGGTPSSLFKHCNDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW30</accession>
    <name>W5LW30_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000337"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000337.1">
        <property type="protein sequence ID" value="ENSLOCP00000000337.1"/>
        <property type="gene ID" value="ENSLOCG00000000310.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155751"/>
    <dbReference type="HOGENOM" id="CLU_123434_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW30"/>
    <dbReference type="OMA" id="SEAHWAV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000310">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF54">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="1">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="1">
        <location>
            <begin position="119"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="166" mass="18032" checksum="94A31330EAF993AA" modified="2014-04-16" version="1">KQELKNTVFRLWSLLQVDPAGSGRVAATDAALFLKRSGLADLVLGKIWDLADAERKGFLNKQQFFVALRLVACAQNGLEVALKSLSVAVPSPKFHDTSSPLLPPGVVPIDSPWAVKPEEKLKYDAIFDSLSPVNGMLSGEKVKPVLLNSKLPVDVLGRVREKAISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWB7</accession>
    <name>W5LWB7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Cytoplasmic vesicle</location>
            <location evidence="10 11">COPI-coated vesicle membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10 11">Golgi apparatus membrane</location>
            <topology evidence="10 11">Peripheral membrane protein</topology>
            <orientation evidence="10 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWB7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000424"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000424.1">
        <property type="protein sequence ID" value="ENSLOCP00000000424.1"/>
        <property type="gene ID" value="ENSLOCG00000000389.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102688013"/>
    <dbReference type="eggNOG" id="KOG2635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017207"/>
    <dbReference type="HOGENOM" id="CLU_019988_0_0_1"/>
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    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="59"/>
            <end position="299"/>
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            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU364018"/>
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        <source>
            <dbReference type="RuleBase" id="RU366052"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51072"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="299" mass="33108" checksum="FD283E5AC947678B" modified="2014-04-16" version="1">MFCCGRSSGPSKALKLGARGKEVDNFVDKLKSEGENIILPSSGKRPSEVSKVLPPPVNMESVHLRVEEKISLTCGRDGGLQNMEVLGMITLRVSEDKSGRIRLHVHNDDKKGLQLQTHPNVDKKLFTAESLIGLKNPEKSFPLNNDVGVLKWRLQTTDESFIPLTINCWPSESGSGCDVNIEYELQEEGLELNDVVISIPVPSGVGAPLIGDLDGEYRHDSRRNVLEWCLPVIDAKNKTGSLEFSIAGQPNDFFPVHVSFVSKRNYCDIQISKVTLVDSDSPVRFSLETSFVVDKYEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWY7</accession>
    <name>W5LWY7_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWY7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000644"/>
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        <property type="protein sequence ID" value="ENSLOCP00000000644.1"/>
        <property type="gene ID" value="ENSLOCG00000000582.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000582">
        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:immunoglobulin complex"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="IgV_Light_Chain"/>
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        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
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        <property type="entry name" value="IG"/>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865241" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="125" mass="13314" checksum="A3B1853D96182293" modified="2014-04-16" version="1">THRTMMPLILLLGTLGLFAQESSRQIVLTQSPAQSVLPGSSVSISCTASSSLISGITSYLNWYLQKPGHSPQLLVCYATTRQSGVPDRFTGSGSGTAFTLTITGVQAEDAGDYYCQQCYSTPLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXU9</accession>
    <name>W5LXU9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LXU9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000960.1">
        <property type="protein sequence ID" value="ENSLOCP00000000956.1"/>
        <property type="gene ID" value="ENSLOCG00000000859.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164014"/>
    <dbReference type="HOGENOM" id="CLU_009579_23_1_1"/>
    <dbReference type="InParanoid" id="W5LXU9"/>
    <dbReference type="OMA" id="CVSFERY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="25069" checksum="DA15A4958D0C3899" modified="2014-04-16" version="1">VKADCVSPLYVINLLAANLLQIATVPIYLASFTDQRVCSWTVVYQVNVAVFEVGLLASIGFMLCISLERYLVIARPVWHHNHHSLSFVSRISLAVWGAAVLLTSIKNFYLAILFLVPLALLLIFCVSTRRALVTLSTLPPEEKKRILRTLALVLFIFIGIFAPHFVLYVITVIYNLAKVDVPQGVTDCQGVLLNIRCLHPLLDPILYFLLRTDVRESLDTVSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYA9</accession>
    <name>W5LYA9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon induced transmembrane protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYA9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001116"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001120.1">
        <property type="protein sequence ID" value="ENSLOCP00000001116.1"/>
        <property type="gene ID" value="ENSLOCG00000000993.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S9XK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182857"/>
    <dbReference type="HOGENOM" id="CLU_124511_3_0_1"/>
    <dbReference type="InParanoid" id="W5LYA9"/>
    <dbReference type="OMA" id="RYASTAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000993">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051517">
        <property type="entry name" value="IFITM_antiviral_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999:SF31">
        <property type="entry name" value="IFITM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999">
        <property type="entry name" value="INTERFERON INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="128" mass="14170" checksum="98818C694DF50143" modified="2014-04-16" version="1">ASMENLKYSPELLPMQGNSYERLKEPRVVTLPSNAAVVTIGPPAKPPRDHIIWSLFSTIYMNYCCLGFAALCYSIKARDRKALGDLEGARGYASTARCFNLTTLILFLITVLVLIILISVGVVYPMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYI5</accession>
    <name>W5LYI5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Si:ch211-246m6.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYI5"/>
    <dbReference type="FunCoup" id="W5LYI5">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001192"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001196.1">
        <property type="protein sequence ID" value="ENSLOCP00000001192.1"/>
        <property type="gene ID" value="ENSLOCG00000001058.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166136"/>
    <dbReference type="HOGENOM" id="CLU_115077_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYI5"/>
    <dbReference type="OMA" id="PCLRYQG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001058">
        <property type="expression patterns" value="Expressed in muscle tissue and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11465">
        <property type="entry name" value="bHLH_TS_scleraxis_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF40">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="69"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="179" mass="19486" checksum="5BE375A20A2F4B54" modified="2014-04-16" version="1">HGSHSVPAQPRYGMKSTGSERAGHPDCGASDLDELDSGSESSDKSTSGCSQHNGKGARRRRTRLAGVSKQRQAANARERDRTHSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLANILLLGEDCLDGQPCLKYQTILQSSTRSSNPAPRPICTFCLGNQRKMLREGEKHSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0K7</accession>
    <name>W5M0K7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cardiolipin synthase (CMP-forming)</fullName>
            <ecNumber evidence="10">2.7.8.41</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a CDP-1,2-diacyl-sn-glycerol + a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a cardiolipin + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="2.7.8.41"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.41" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01034567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0K7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001919.1">
        <property type="protein sequence ID" value="ENSLOCP00000001914.1"/>
        <property type="gene ID" value="ENSLOCG00000001653.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154169"/>
    <dbReference type="HOGENOM" id="CLU_081147_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0K7"/>
    <dbReference type="OMA" id="YKGLPCR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001653">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="242" mass="26601" checksum="EB48E913009C915A" modified="2014-04-16" version="1">NSSLSLVSDVFQCAKLLLLNKQESWVKLREFARKNAANVLSLANMIMGLSSILCSVNGDQISACWLVLIGYLLDLADGAVARQLNACSAVGAKLDDFADFTTFGIATALLLPRTSLLDSLLCIFYVLAVFVRLCFFSSAGIPFTYRGLPCIYSSAVLACTFLLTGGNMVILRVTAVAMILFMVDQGFYPHDRVLESQTWKKMVFAGGVFMVFCVSSFLTCIYYLLWSVSYILFPTALWSSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0K9</accession>
    <name>W5M0K9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger CCCH-type with G patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0K9"/>
    <dbReference type="FunCoup" id="W5M0K9">
        <property type="interactions" value="1190"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001916"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001921.1">
        <property type="protein sequence ID" value="ENSLOCP00000001916.1"/>
        <property type="gene ID" value="ENSLOCG00000001659.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694706"/>
    <dbReference type="KEGG" id="loc:102694706"/>
    <dbReference type="CTD" id="84619"/>
    <dbReference type="eggNOG" id="KOG2185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000732"/>
    <dbReference type="HOGENOM" id="CLU_040504_1_0_1"/>
    <dbReference type="InParanoid" id="W5M0K9"/>
    <dbReference type="OMA" id="QYTRGIG"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001659">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20384">
        <property type="entry name" value="Tudor_ZGPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000071">
        <property type="entry name" value="Zinc finger CCCH-type with G patch domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297:SF1">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="15">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="375"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="522" mass="57094" checksum="57CC618F41D2FD60" modified="2014-04-16" version="1">MDEGSLEAAIETYHAQLQQVETALGAGLEPSQQSDLLQLKEDLLQLIELTEASLVSVKKSRLLASLEGQPPAGDPGMRDEFAAFYAAVSEPSCAEPSTSESAPAAQRSGGGSGSPGSQQAAEEEEEEEEEEDVMSGMKVCAPYRTSWGTLEYHNAMVVGTEDSEDDEPQVRVLYVHPTQKSMKPCPFFLEDKCRFMDSCRFSHGEVVLVSELRPFQESDLSSLQEGSACLARHEDGIWYPAKITDVDSGYYTVKFDSLLLRSAVVEADGIIPPLRKDDPPSSESEDEDMVEDSAYARVVDLASEANGDWAPSSCSEFGGWEAHTRGIGSKLMAKMGYEFGKGLGRNAEGRVEPVLAVVLPKGKSLDQCAAILQKRAPGNPGGKLAKPRKKGKTRTGSSQPRTDVFDFLNSQLGDRNRPGAAEAGVPLAERNSKETYRGGETTKRSLNVRLYQAAEKVAQTEREIGRITESLSRNLGRDKAMTSHLEERLSAARKQLVQLKAQEVHIQKERKKADTHKKMTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2P7</accession>
    <name>W5M2P7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2P7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002655"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002661.1">
        <property type="protein sequence ID" value="ENSLOCP00000002655.1"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
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        <property type="entry name" value="GENE 9195-RELATED"/>
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        <location>
            <begin position="394"/>
            <end position="441"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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            <dbReference type="Pfam" id="PF07699"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002655.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="701" mass="71647" checksum="FCD86EF2C13B9164" modified="2014-04-16" version="1">MGSGRPEDCSPCPEGSFCREGSAKPSPCPASTFRRQKGGKQAEDCSLCPAGYSCPHLATVQPHSCGAGSYSDQGSAACSPCLPGHYCSHETTSREAMLSTMVCPAGLICSAGLDREPQRAAILCPKGFYCPGGSIDPNPRPCPNGTYSDQPGLRDLSDCSPCPEGKYCYSDQQPHIHGLSQPTGQCPDGYYCPVGTGYPYSFPCPPGLYRNSSHGHGGGDCVPCAPGHYCAQAATHAPAVCPKGFFCIEGSSTPEPCEEGTYSPRLALRQGSECTPCGGGRYCTGVAQTEPTGLCKEGFYCKERARSATPADGQTGGLCPVGSFCPAGSAAPSPCPTGTFSNSTGLQSPGQCAVCPPGTHCSGSNNTSPAGPCAPGYFCTGGSSSSYQHEAQEGHYTLLGAVRAEPCPLGTFQPDRAQSTCLPCLQGHFCNQTGAVQPTQCPPGHYCPPGAVLPSSCPVGSYSAQGGAEDVGHCSPCDPGMFCGSSGLSAPQGVCAPGFYCAGGATTATPVAVSSGDVCPAGYYCPLGSRHPRAHPCPPGTWSNAVGAHNESSCWPCPAGFFCNGTGLRQATGLCAPGFYCLRGATSPMPLDGVTGDKCPPGYHCPQGTAVPQPCQDGTFSNTTGSAECLECPPGFYCLLDQGVQMCPEGHYCPGGTGMDVLPCPPGTYSAAPGHSQLEQCLLCPAGMYCEEWGLAHPTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2T2</accession>
    <name>W5M2T2_LEPOC</name>
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        <submittedName>
            <fullName evidence="5">Lipocalin 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000002690.1"/>
        <property type="gene ID" value="ENSLOCG00000002295.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_094061_3_1_1"/>
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        <property type="component" value="Linkage group LG21"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Calycin"/>
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        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
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        <property type="entry name" value="LOC555483 PROTEIN-RELATED"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="chain" id="PRO_5047039585" evidence="3">
        <location>
            <begin position="20"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="4">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="191" mass="21741" checksum="9877A110F30D42A2" modified="2025-10-08" version="2">MGVQLTLLCALSASGEVQPQKDFDLQKFAGRWYRVGLAYDSPAFAPFRHMLRISKGILTPMENGNVNVTMWGARSSGCRSRMYMYEKTAVPGQFTYFSTSHNRVKDITIVETNYDEYALVMKHKKMDKEYSQVALYGRTERLRPELLEKFREFALAQGFSPESILTPPPTEDCPPGEWGVCLCFLTQRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M398</accession>
    <name>W5M398_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G-protein coupled receptor 54-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626678.1">
        <property type="nucleotide sequence ID" value="XM_006626615.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M398"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002856"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002862.1">
        <property type="protein sequence ID" value="ENSLOCP00000002856.1"/>
        <property type="gene ID" value="ENSLOCG00000002431.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683909"/>
    <dbReference type="KEGG" id="loc:102683909"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286969"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_4_1"/>
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    <dbReference type="OrthoDB" id="2132067at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in brain and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046887">
        <property type="term" value="P:positive regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15095">
        <property type="entry name" value="7tmA_KiSS1R"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KISS1 receptor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008103">
        <property type="entry name" value="KiSS_1_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="KISS1RECEPTR"/>
    </dbReference>
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        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="77"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="382" mass="42831" checksum="1C8889D6C8997646" modified="2014-04-16" version="1">MVETTPTAVNSANNSGLSFCHNGLDLSIPSEGSEAAFSLGSGCNGSIVKEQPSPPLLVDAWLVPLFFALIMLVGLIGNSLVIYVVTKHRQMRTVTNFYIANLATTDIIFLVCCVPFTATLYPLPSWVFGDFMCRIVNYLQQVTAQATCITLSAMSVDRCYATVYPLQSLRHRTPRVAMAVSVSIWLGSFVLSVPVAIYQRLESGYWYGPQTYCTESFPSAYHQKAFILYTFLAVYLLPLITICFCYAFMLKRMSRPVVEPVDNNYQVQLLAERSEAMRTKISKMVVVIVVLFTICWGPIQFFILLQAFYSNFRRSYATYKIKIWAHCMSYSNSSVNPIVYAFMGANFRKSFKKAFPFIFKQRVGTTGVAAVNTEMQFVSSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3G4</accession>
    <name>W5M3G4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kirre like nephrin family adhesion molecule 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193012.1">
        <property type="nucleotide sequence ID" value="XM_015337526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3G4"/>
    <dbReference type="FunCoup" id="W5M3G4">
        <property type="interactions" value="547"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002922"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002928.1">
        <property type="protein sequence ID" value="ENSLOCP00000002922.1"/>
        <property type="gene ID" value="ENSLOCG00000002470.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687316"/>
    <dbReference type="eggNOG" id="KOG3510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157126"/>
    <dbReference type="HOGENOM" id="CLU_013520_1_2_1"/>
    <dbReference type="InParanoid" id="W5M3G4"/>
    <dbReference type="OMA" id="EDHMASI"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002470">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05759">
        <property type="entry name" value="IgI_2_KIRREL3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF49">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="476"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="99"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="202"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="287"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="371"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="696"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="699"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="730" mass="79723" checksum="747FB71278D7C809" modified="2014-04-16" version="1">MFAQQPQDLVVVAGQPVTLHCAIPGYHGIVLWIKDGLALGVGRDLSGYPRYSVVGDHSAGEHHLRIQRAELMDDAVFECQAIQAAMRSRPARLTVLVPPDDPVINGGPVVSLRAGDPLNLTCHADNAKPAASILWIRNGEVLNGAMYSKTLVRDGRRESTVSTLYVSPSNIETGQQIVCRASNKAVPGGKETSVTIDIQHLPLVNLSVEPQPILEGNLVKFHCSAKANPPVTQYRWAKGGSVIKEVTGDVYEVIVDHSFFTEPVSCEVTNPLGSTNISRNVDVYFGPRMAAEPQSLLVDLGSDAVFNCAWTGNPSLTIVWMKRGSGVVLSNENILTLKAVRQEDAGKYVCRAVVPRVGADEKEVSLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETVSTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGGSQESLPMAVIIGVAVGAFVAFIVLMATIGAFCCTRSQRNLKGVVSAKNDIRVEIVHKDHSAARETEEHAAIKQLMIERGEFQQESVLKQLEVLQEEEKEYQHIKDPTNGYYSVNTFKEHHSTPTISLAGTQTTELRSTTTATMGKQRVPTGMSFTNIYTTLGAGPNRLYDYSQRFVLGMGSSSIELCEREFQRSSMSDSSSFLDTQCDSSVSSYSKQDGYVQFDKASKASASSSSHYSQSSSQNSDLTRPLQKRMQTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3S0</accession>
    <name>W5M3S0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat domain 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M3S0">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003028"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003034.1">
        <property type="protein sequence ID" value="ENSLOCP00000003028.1"/>
        <property type="gene ID" value="ENSLOCG00000002556.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQG5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154742"/>
    <dbReference type="InParanoid" id="W5M3S0"/>
    <dbReference type="OMA" id="MTAVENC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002556">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053210">
        <property type="entry name" value="ANKRD12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149:SF14">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="543"/>
        </location>
    </feature>
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        <location>
            <begin position="565"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="682"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1074"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1295"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1672"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="577"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="618"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="629"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="704"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="771"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="958"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1022"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1040"/>
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        </location>
    </feature>
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        <location>
            <begin position="1295"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1314"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1672"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1696"/>
            <end position="1711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2028" mass="229614" checksum="544C9EE8E920EA3A" modified="2014-04-16" version="1">IEKSGFGVAFSHPTSVRNTNFETRLFIKKSKDKISPFTKTPKVDRSELLGKEMKQKSSMKRKLPFTVSPPRHEERDSDTDKDGPDKKKVKKETGSKKSTPVNIMFGYPLSERKQMALLMQMTARDNSPDTTPNHPSQATPVQKKIPSTSSRQKDKVNKRNERGETPLHMAAIRGDVKHVKELISLGADVNVKDFAGWTPLHEACNMGYYDVAKVLIAAGAEVNTQGLDDDTPLHDASSSGHKDIVKLLLRHGGNAFQANNRGERPVDVADSEELEHLLKGEIPLSDEEDSSSEFEDPLSVNPSSVDDNMEYSDMEKDSENKQLNPAKASASGLDEYEFKDDEEEEDLSKALNDRHILRREMRQREKDEKERNNFMTKQEKNDQTNICRSKKQKSSRVLFCNSESSGDETHQERKTCSPTCSLVGAADGHKTDVRTKKDLTLTVDHKEKGKVKKKFKNQSKNKENQEVKEDGKENSKVAFFSTAPGLETPEKTREDDTFKMSFSPKDDSSVHLFHLPSVKSPKLNHSVTDKQASPLKQENAKTCLSTGVSESPLQMENVNFDHYTEADYNTESSSSKGLKHKEKSKHHQKDMGIDVEDENSTPCKEDHLGTSLDSSEGTLRKMDKEGKVIKKHKLKHKEKDKHRKEYEGAKDKHRKKEMRKEGIRNLEFDREFWKENFFKSDENDESFSGKADSSETGAPQMSPVKEEKGSKEKHVSKEKRLKEDRDKDKSQKRDKKENLFKEEKGKEIKDEKIDLLTSDKVPEDSLQDVPVVKEELEEHLLTEKDTDNDQHDALEKGAREKSDKKYSVKEKETEKLDRKHPDKEKKLKTEQNFLDKPDSHNSTDRWKEKDKTGPSHSFGEKNYKESEKLKAVLSTKKPEENKKSKDKVERKYEKEKQDKERYFGDNRDKEKNGTDKKGKTSEKGVDLGKPDRTKEKEHEKKKKEKIKDVTSSSNVKIFPEERKPSVSESSKMLHDKTFTKLKDDGLKVPEKDRRDKDREIDRYKERDKHKDKTQPNKLNKTKTNELEADKLKLKPSPAPKDARPKEKRLVNDDLMQTSFERMLSLKDQEIEQWHRKHKEKIKQKERERMKHKPGLDLNKLKNKERFKILPNEVGNSKELLRSKSSELSDLHTREKVLKEATGARSFSLDAKNLPNSGKSGLGLENSLSRSPRPEHEKSGLVSRSVSMISVASSEDSCQTTVMTPRPITEYDSDFTLEGSDSQLSFSLPLSVTHAKSPVIHETETESLLDSAQRTKASLSNKQAAHVRTTSAEDAKPAAVDGRLFVEDCRYSSLAQSNSENLRTSVAESSLAPAQENQNSPNSVFPNLNNASSLTEGPSDSGIEGHLCSGARSTHLSLPSSPLPVPNSSDQMSEDSDEALNQLINPPQTDCIKETDQEIEKLPNSELLPDKNTPCVSTEDSRIEFSSEKLISECKMKENKDSPSVNALESLPLQHSLSSNFRSSSDSTSEGQDAVEPDSSKLNLEVSHTGEEDLVMVTEQKEKPVLIVDLHSSAGSKVDEQNMENSLSNLSVDAVTECVNGPGQCASLSCTVTQQIGNNTTIKANTMCEGAMDTQEDMETDDTLNTEDTKLKPRNKSVHENLGDLQLDKYESNQPNFEANCLDLEHKIMDIAEAAEDLENNDLLDASERGKLTDTVLQAQSLPDVKFSAEAHVKTEEEEMITDRECAEETSLHQEESQGTPSTLQENSQTGPTKDPSVGGPQTGDKEADFSGSIKAKIRLTEDEDLQVHHPRKRKMPRVPQAVQANPTLQQAKEKTQQSLAAIVDSLKLEEIQPYQTERANPYYEFLHIRKKIEEKRKVLCSVVPQAPQYYDEYVTFNGSYLLDGNPLSKLCIPTITPPPSLPQPLKELFKQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNMPQDAQTDDGKTSVRDRFNARQFMSWLQDVDDKFDKLKTRLLMRQQHEAAALNAVQRLEWQLKLQELDPAAYKSISIFEIPEFYVPLVDVNDDFELTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3V8</accession>
    <name>W5M3V8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01009477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3V8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003066"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003072.1">
        <property type="protein sequence ID" value="ENSLOCP00000003066.1"/>
        <property type="gene ID" value="ENSLOCG00000002592.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158448"/>
    <dbReference type="InParanoid" id="W5M3V8"/>
    <dbReference type="OMA" id="CSMLANP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002592">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF3">
        <property type="entry name" value="TUMOR NECROSIS FACTOR ALPHA-INDUCED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="11">
        <location>
            <begin position="580"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="11">
        <location>
            <begin position="639"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="11">
        <location>
            <begin position="739"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="413"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="771" mass="84624" checksum="E5AFFE8D5F4F6D4E" modified="2014-04-16" version="1">MAERKRLPQSLEDSNLTKALFLRQNIAADLRRQRRSKPAVHHLDTLHPYSVHLSDCSASRPRIAEIVSESLFDLQLRRHLADSGCEQASTLSPLRNSGTGENNSLLDAVSLYMWGVRDSDLVLRTALYNTMDESSPAELYMLKRLLNSVMVNSAPFPFPKRKLLCQLGSEEWERIVRLVDPKSEGVAPMANSYLDIYLFVLANVIRRPIIVLGGNTKEVTPDTSESVQGPAGIYLPLLWDVEKCYHFPVVLGCVYPYSCFAPLVDFSAPGQETEILPLVTHTKNGPKDMSVHFLPECEETTKQICLQNYLQLTAVSVGKSTVPATSFCRLKGNNLPENLNLVQDYFRLVNYVCGQLSQGAEGAQAGGREQGNRSTSPSFSITRDKCITEHCLYFASKLTWPFCHECSELSNCKDTPSMAPHPRHRAGKQETREYSSGSSFYGDRPSKVQLPAGPASAPSGHTPAPDPKSQQHRSNAGHLGQAAARLDALEPKTGACPHPRTEGGEAREARRPGAQKDPAAAGPVDLLCGRCIACKRETRTFNGLCFDCLQRKAQAAEPREARLAQALAPGWQGAGSRGQQPPGERCITPGCVYFGTEQHSGYCTVCYCSRGLPLPEPPGCEADRQQAPAGQPPQIASTLRNMPKCRAAGCGMLGNPSFDGFCEKCFLTHSRRAAQSRQIYFITVTAIKPAARSPCNTFHSQTLALAKQEPLHERLQESACGEMREGPSGPSGKAQAAAALDRRPCRHRSCPNFGNSRCEGYCNHCYKKYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5M405</accession>
    <name>W5M405_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01037017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG5"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867575" evidence="1">
        <location>
            <begin position="23"/>
            <end position="183"/>
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            <dbReference type="SAM" id="SignalP"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="183" mass="18892" checksum="241DD58020704C0C" modified="2014-04-16" version="1">LQMDQRLSVALLLLGLTVLVDGQTVAAPFTSPQGNITRNGCGATKFCIETPTGCDPASSGNCLFASAARDTTSTTSISFNLQGNSSGYIAAILTTAGNNSTGDAFVCAQNASDTLFYHTVYSSNGSSLSPNIFNNTVYNVTGSANNNIISCTFVARNLTISNGKATTLYNISLAVGDFTDGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
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    <name>W5M4E3_LEPOC</name>
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        <recommendedName>
            <fullName evidence="3">Protein FAM118B</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">May play a role in Cajal bodies formation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear body</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the FAM118 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="353" mass="39837" checksum="759CAD125FF13A86" modified="2014-04-16" version="1">MASVAPVKTEKRPASADADSAAKKPRKLLPSLKTKRAPELVLVIGTGVSSAVAPQVPALRSWKGLIQALLDAANDFDLLEEEESRRFQQSLQEDKNLVHVAHDLIQKLSPRTGNVRSTFFKDCLYEVFDDLESKMEHAGKHLLRSVLQLMESGALVLTTNFDNLLEIYAAHQGTKLESLDLTDEKKVLEWAQEKRRLSVLHIHGVYTNPSGIVLHPAGYQNVLRNTEVMREIQKLYETKSFVFLGCGRTVDDTTFQALFLEAVKHKSDLEHFMLVRREDVGEFKKLRDNMLDKGIKVISYGNEYADLPEYFERLANEICTRDVAVNGWGSPDQEDVNQNGLSSQNNLFQDCHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4I2</accession>
    <name>W5M4I2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger and BTB domain containing 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000003297.1">
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        <property type="gene ID" value="ENSLOCG00000002796.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF24">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="277"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="675"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="678"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="728" mass="80752" checksum="86BB460828FBB0A1" modified="2014-04-16" version="1">MSGERNRRSLAFRGGGLAVTGASGVGGGHGNSWEAQAWQDRHLNGNRPEQEEDRDLGARGASLGTLEGGGSISDSTEAEEEEDAEGAGWGLGLREDERVAHPGGDGDGPGGSCTYRQGPLKDTGAVLKECVGELKKKKRSCRPSVFRLAPFRQTQFFKHMPFYASWLVQFPIYKYASHPLKVVLSCQMCAHGLGQDRQSRFKGSVSFFVHSKMFTCLSCDHDHRKPSGSQVSPCQDTEKPGDADGEDAEYRNKPNENSYCYQLLQELNKQRKNGILCDVNIVVSGQVFRAHKNILVAGSRFFKTLYCLTKNESCDQTTITHLDVAAVQGFSVILDFLYSGNLLLTSQNAIEVMSVASYLQMTEVVQSCRAFIKDALNISIKSEAPDSVVVDYNKRRAVTKDGKGADKKPSNFWATSILSKLSIKASGHIKEEPSDVEGSIGESYPLSSSGWGYDNSSESAENEPQGPGPVFVWNDAAIQTGRRDFKQEPGSGRRKNQTTRRYVYNMTPEPEESFDEGMFVQPSSSYSKDDLQFFSENAELANQIQYSLLQDAQPRESWENGESSASVVNKLKCPHCNYIAKHRRTLKRHLIIHSGVRSFSCDICGKLFTRREHVKRHSLVHKKDKKYKCMVCKKIFMLAASVGIRHGSRRYGVCVDCADSHQGSQEGLDAMQELEFSREDDFEENEAGEGEGEGEEDLGEEGEEQAENDQAHWEEADASEVCVTLEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4P1</accession>
    <name>W5M4P1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4P1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003349"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003356.1">
        <property type="protein sequence ID" value="ENSLOCP00000003349.1"/>
        <property type="gene ID" value="ENSLOCG00000002851.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="HOGENOM" id="CLU_1964563_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4P1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002851">
        <property type="expression patterns" value="Expressed in ovary and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF511">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="76"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="128" mass="13989" checksum="3E2CCCEE419BB041" modified="2014-04-16" version="1">VPRSVCSDSCSPGSRKATRRGQPVCCFDCVPCADGEISSQTDSLDCTKCPLETWSNKARDQCIPKEVEFLSYSESMGMVLTVVSTLGACVTTAVSGVFIFFRNTPIVRANNMELSFLLLLFLILCFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4Q2</accession>
    <name>W5M4Q2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interleukin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the IL-15/IL-21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4Q2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003360"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003367.1">
        <property type="protein sequence ID" value="ENSLOCP00000003360.1"/>
        <property type="gene ID" value="ENSLOCG00000002859.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SES1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235110"/>
    <dbReference type="HOGENOM" id="CLU_142925_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4Q2"/>
    <dbReference type="OMA" id="ETQKAQC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002859">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045954">
        <property type="term" value="P:positive regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.70">
        <property type="entry name" value="Interleukin-15/Interleukin-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003443">
        <property type="entry name" value="IL-15/IL-21_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14356">
        <property type="entry name" value="INTERLEUKIN-15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14356:SF2">
        <property type="entry name" value="INTERLEUKIN-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02372">
        <property type="entry name" value="IL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865548" description="Interleukin" evidence="9">
        <location>
            <begin position="20"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="147" mass="16512" checksum="BDFE95C3C48B58AD" modified="2014-04-16" version="1">MVKLIVCCLLAVLSSSVMGAPADDTLATLQELLKDLKHLSKNIKHDGRMLNTPLIEDIEDCCIPSALRCFKEMLPGLTTTDEQKSQKVKKMCYKMSNKTIDRTGNVCTPEEVEKASCRACDSYEMKDSKDFLNAMEKLLQKSVMKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M615</accession>
    <name>W5M615_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:dkey-89b17.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035967">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M615"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003823"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003830.1">
        <property type="protein sequence ID" value="ENSLOCP00000003823.1"/>
        <property type="gene ID" value="ENSLOCG00000003238.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164729"/>
    <dbReference type="HOGENOM" id="CLU_002678_44_18_1"/>
    <dbReference type="InParanoid" id="W5M615"/>
    <dbReference type="OMA" id="QSNSAHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003238">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000729">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001345">
        <property type="entry name" value="zinc finger protein 646"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002353">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF127">
        <property type="entry name" value="BLOODY FINGERS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="661"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="783"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="811"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="851"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="878"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="906"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="564"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="730"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="826"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="924" mass="101465" checksum="19FBF412554EC69B" modified="2014-04-16" version="1">PEMQDQARTTGFQCKQCEVVCPSWPGLLEHVESHYQHEEERRFWCEQCGRGYRHAGSLVNHKKTHEVGSFQCPVCARELSNPLALKNHLRIHTPGKNYPCPVEVGDVLDPPWGSRDRDTFLEQDPSEQADSQASVTDSSPDCGLQPDAHLEAGVDKPSTEEDRPFKCNQCEKTYRHHGSLINHKKSHQLGAYECHVCYKQFNNLAALKSHLRIHSKSKGRPAVASPFSGAAAAELSAPQNGDASHFCHLCQVAFSSEGEFQDHVLLHNSTSLSLGPPDDFSDDLSYDYTGVGSPDDGVYAHPPLIGRSDEKEEAEEEESVAAVNGQMYTCAFCGESYSDLESLKAHYLTHDAGLQDEDVPPEQEEGNPEGTPAAKGEPKVNQAAAEVTGSDERRFKCQLCGKSYRHAGSLINHKRSHQTGVFQCSICQKHYPHLAALKSHLRIHKSKPPALLLSSEGDWLSPEPLTLEGQSYVLPFSSQVPDSGLAMPQDVALPAPPDDAADASGSVCFPPFDSGLASSCPASPPTPDPGFQAERHMCADCGETYSDIAGIKSHQCPRRENLNGLTGSGGFREAGDNADVEEDGNESPVPVGSRRRKRGPAQKHVEAEERRDAEEYGELCQCSVCGNHYASLSALRSHLRCHTLPGGAGRSPEAEDEGGLLICSACGESFLTERDLEDHQLSHESELAPLPPKPESKSHACARCGAVCDDYYPLGAHRCAEDAGGEAAEAADAGGEGKAESPGALQEGPDAADRPHRCEQCGRAYRHAGSLLNHKKSHKTGVFRCFVCQKRFYNLLALKNHQRVHFDIKRHKCSECGKAFKIHKQLVSHQRTHEENRARAKELNRQLQRLLQCEHCGRTYRHAGSLLNHKNSHKTGSFSCAACQKQFSNLMALKNHRRIHTEPKRYQCPDCGKAFRVSTQLICH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M622</accession>
    <name>W5M622_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC1, PSMC2, PSMD1 and PSMD6. Part of transient complex containing PSMD5, PSMC2, PSMC1 and PSMD2 formed during the assembly of the 26S proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5B/HSM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M622"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003837.1">
        <property type="protein sequence ID" value="ENSLOCP00000003830.1"/>
        <property type="gene ID" value="ENSLOCG00000003231.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684172"/>
    <dbReference type="KEGG" id="loc:102684172"/>
    <dbReference type="CTD" id="5711"/>
    <dbReference type="GeneTree" id="ENSGT00390000013040"/>
    <dbReference type="HOGENOM" id="CLU_043710_0_0_1"/>
    <dbReference type="OrthoDB" id="10250600at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003231">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000208">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019538">
        <property type="entry name" value="PSMD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554:SF10">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13554">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10508">
        <property type="entry name" value="Proteasom_PSMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="504" mass="55302" checksum="526B4EC8F714941D" modified="2014-04-16" version="1">MATSIEGLLSELSGLDEPLEELRNLKTAVLAIPLNILGETVNGLRLEVIFSLLNTNDREQIELCGAILSRILQALDPVILAQNLKTELQCGLNHPDDLVKILAMTQIGRIVEHPEAVTEILNSQELVKQIINCIGGEKISVAKEAIKALSKMAQTKAGLDALFMGNLLKYLKDVMAISDVVRYRVYELVVEISSVSPVSLGYCANSSFISQLIGELTGDDILVRATAIEMVTTLAQSQHGRQYLAQQGIVDKISNMITGAESDPFSGFYLPGLVKFFGNLAIMDSPQQICECYPAFLKKVFEMAVGQDPTMIGVALDTLGILGSNIEGKQVLQKTGEKFQDVLKRMSNVARNATTELRVRSLEAVALLLTLPAEQQTEDLLGLTESWFNSLSSQPMEMLKSISTQPFPELHCSALKIFTGMASQPWGQKKMIDTPGFVEFIVDRSTGPDKESKDAKYELVKALVDSKTTAEIFGNQHYLRLRTYMREGPYYVTAVSSVTVEGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6D5</accession>
    <name>W5M6D5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 217 member Bb</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6D5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003943"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003951.1">
        <property type="protein sequence ID" value="ENSLOCP00000003943.1"/>
        <property type="gene ID" value="ENSLOCG00000003336.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R40C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154543"/>
    <dbReference type="HOGENOM" id="CLU_037024_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6D5"/>
    <dbReference type="OMA" id="CHMRYPL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003336">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029266">
        <property type="entry name" value="FAM217"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22145">
        <property type="entry name" value="SI:CH211-266K22.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22145:SF2">
        <property type="entry name" value="SI:CH211-266K22.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15344">
        <property type="entry name" value="FAM217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="433" mass="46703" checksum="39B3CA0A55483C12" modified="2014-04-16" version="1">MSPVLQERPGTTALKKGSSKDRIRTKASGNIGQVTSPQTQIWSCLFDSSKKGKAILSLRAQKKSGAQPKHGSPRQGKELPQAAERVKFSCKSLNSTPRRCRISGKEDGKPLLSPLAEGSWREKVLPGSCSGSETDVGSLGSGKSRPLLSLPLVPPQEKRCPELGTGQPPSLETLKLYAGEEDDGDNASDLSDSERLPVLPSPCTPPQLHLRAEVVDPSDLHPYFPGARGGGSPGYSYPDFLPPPFSSWSLRQLALFLNTEGKGAPRPKPVGQLERYLERLLQLEWLQIQTVQAEGSKSPSLVSAARPRPHTAPPAHLSSPKSLRQCQRAFPLAFLSCLGNPSAPQLSRPACPHCRVHYPFCNGACRAYAYQRHSRLSPLLERRAGACGPQRRSSSESRVASSDPRPGVQGQKPESPSPGAGHLKRMQAAGNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M990</accession>
    <name>W5M990_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Prostamide/prostaglandin F synthase</fullName>
            <ecNumber evidence="13">1.11.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxiredoxin-like 2B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>prostaglandin H2 + [thioredoxin]-dithiol = prostaglandin F2alpha + [thioredoxin]-disulfide</text>
            <dbReference type="Rhea" id="RHEA:28214"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>prostamide F2alpha + [thioredoxin]-disulfide = prostamide H2 + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:26373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:53081"/>
            <dbReference type="ChEBI" id="CHEBI:53082"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peroxiredoxin-like PRXL2 family. Prostamide/prostaglandin F synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.20" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01009756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M990"/>
    <dbReference type="FunCoup" id="W5M990">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004949"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004957.1">
        <property type="protein sequence ID" value="ENSLOCP00000004949.1"/>
        <property type="gene ID" value="ENSLOCG00000004121.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165161"/>
    <dbReference type="HOGENOM" id="CLU_094994_0_0_1"/>
    <dbReference type="InParanoid" id="W5M990"/>
    <dbReference type="OMA" id="QRPVCND"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004121">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047017">
        <property type="term" value="F:prostaglandin F synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02970">
        <property type="entry name" value="PRX_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000243">
        <property type="entry name" value="Prostamide/prostaglandin F synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF28">
        <property type="entry name" value="PROSTAMIDE_PROSTAGLANDIN F SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="241" mass="26648" checksum="5B2CC68EE25A6920" modified="2014-04-16" version="1">MLQYSLYYKTTMERKRKNGYSTKFEAAVIAVTLCYNLQDKMSGLDLAKIGVNLLKNAATGEMVELQALWQDQAVVLFFLRRFGCQICRWTAAEISKLKEDLSQNQVALVGIGPEETGLQEFKEGGFFKGDLYIDEKKQCYKDLGFKRYSALSVVPAALGKKVRDVVTKANAQGIQGNFSGDLLQSGGMLIVGKGGEKVLLHFVQDSPGDYVPLETIVQTLGIKASVQPGQRPQCNDEVCTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9D5</accession>
    <name>W5M9D5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hyaluronan synthase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9D5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004994"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005002.1">
        <property type="protein sequence ID" value="ENSLOCP00000004994.1"/>
        <property type="gene ID" value="ENSLOCG00000004185.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010337"/>
    <dbReference type="HOGENOM" id="CLU_029695_3_0_1"/>
    <dbReference type="InParanoid" id="W5M9D5"/>
    <dbReference type="OMA" id="YGVARIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004185">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050501">
        <property type="term" value="F:hyaluronan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913">
        <property type="entry name" value="HYALURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913:SF4">
        <property type="entry name" value="HYALURONAN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="544" mass="62489" checksum="6249C39F27F38819" modified="2014-04-16" version="1">TPLEPMESKQLLRRLYGVARICVTLAFALLVLGGLIYAYVAGTRLASAPYSVVSFGVYGVLLGLHILVQSTFAFAEHRRMRRRSLACSYRRSVALTISAYQEDPTYLRECLLSVRSLCYPQSKLRVIMVVDGNSPDDRYMMDMFQEVFRDKDPGCYVWQNNYHSWDPRAAAGGEGDPQREEVEALIQSRPFVCVMQKWGGKREVMYTAFKALGSSVDYVQVRAALPLQESTGQHTASKIVEDRNLYQSCSIKNTMYNKLYTENILHTRPVSLKRCCSFTSCVENISGVWGGFPTDHMYSPPSAWLCAGPLPPDPSPSAHRYTARSRCYTETPAQFLRWLNQQTRWTKSYFREWLYNALWWHKHHLWMTYESIVAGVFPFFVTATVIKLFYSGSLWHILWVLICIQVIGLAKSLYACLLRKNGVMVFMSLYAMLYMTSLLPAKYFAILTMTKSSWGTSGRRKLVGNYMPLLPLSVWGAALLAGLCYTIYRESRCDWSSQPKLAEVPFLIYGSVAYVCYWAAMLLLYWVWFRRCCRRRSMSYDVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9F2</accession>
    <name>W5M9F2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9F2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005011"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005019.1">
        <property type="protein sequence ID" value="ENSLOCP00000005011.1"/>
        <property type="gene ID" value="ENSLOCG00000004196.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ5W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163299"/>
    <dbReference type="InParanoid" id="W5M9F2"/>
    <dbReference type="OMA" id="HCNGLET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004196">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF487">
        <property type="entry name" value="SOLUBLE SCAVENGER RECEPTOR CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865735" description="SRCR domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="239"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="347"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="455"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="556"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="633"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="665" mass="70612" checksum="1ACE229106F5B144" modified="2014-04-16" version="1">MSLLLILGVLYLLPHGEAINVRLVNGHNNCSGRVEVFRNGQWGTVCDDNWDINAAQVLCREVGCGQHSLIVPLHGAYYGEGHGPVILDDVKCTGTESSLLNCKTGETKNQKCDHSEDAGVDCSVKKVPIHKGIRLVNGNNNCSGRVEVYQRGQWGTVCDDHWGKADAEVVCREVGCEGNGSSVAVHGSYYGKGSGVIHMYGGCSGNESSLLSCGAMRRFHCDHSEDVGVDCIGLETIKVRLVNGSNNCSGRVEIFHRGQWGTLCDHSWGSTTAQVACREAGCAGNGSAVAVYGAHFGQGSGPIIMTEFCSGDESSLYECGLHGMGHYGCQHTEDAGVFCSGIDTLKVRLVNGSNNCSGRVEVFHGNQWGTVCGHSWDTNGAQVLCREVGCGEAENLIPLHGAYYGEGSGPVFVQGHCTGDETSLLQCGLRTVRYFGCDHSDDASVDCSGTENIRVKLVNGHNNCSGRVEVFHKGQWGTVCDRNWNSEAARVVCREVGCGDRVSAVAVHNAHFGEGTGTSYVHGHCNGDEPSLLSCGLRDRGYSSCGHTEDAGVDCVGLCKKSNSSVKTPQSCSGRLEVFLSNKWGTVCDDNWDKAAAEVVCRQMGCGVGGSAIAIHSAHFGEGSGPIMLLNHCSGNESLLQSCGIQYMEYQGCYHSEDAAVDCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA53</accession>
    <name>W5MA53_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial ribosomal protein S22</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216500.1">
        <property type="nucleotide sequence ID" value="XM_015361014.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA53"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005270.1">
        <property type="protein sequence ID" value="ENSLOCP00000005262.1"/>
        <property type="gene ID" value="ENSLOCG00000004403.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694311"/>
    <dbReference type="KEGG" id="loc:102694311"/>
    <dbReference type="CTD" id="56945"/>
    <dbReference type="eggNOG" id="KOG3890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006095"/>
    <dbReference type="HOGENOM" id="CLU_053702_0_0_1"/>
    <dbReference type="InParanoid" id="W5MA53"/>
    <dbReference type="OMA" id="GYIELTL"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004403">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019374">
        <property type="entry name" value="Ribosomal_mS22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10245">
        <property type="entry name" value="MRP-S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="341" mass="39186" checksum="358F36A464A0D4A2" modified="2014-04-16" version="1">MAAFSATRCLFGYSAQLRNAARTPHTLLRCARKMLCSESSAGNSSPDVRKPEFSDEVIQDILTKITGMDLHKIFRPIKQELKPPIYKLMTDKQLEEATQQAVEVAKKNLKMPPVLPERKPINSVLAEDKVLEGLETAKYVFTDITYNIPHRERFIVIREPDGTLRKASWEERDRMLQIYFPKEGRRITPPPVFKEENLKVVFLQDRHQDILDLCLVQFEPDSSEYRRVHQLTYDDIEKQGKYDLLRSTRHFGGMVWYLVNAKRVDGLLIDMIQRDLIQDAASLVHLYHIVHPHCQSAAEATEKQVDGIGLVKIFAKTESQKSGYIELALQAYQETTLESSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAP2</accession>
    <name>W5MAP2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tight junction protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005451"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005459.1">
        <property type="protein sequence ID" value="ENSLOCP00000005451.1"/>
        <property type="gene ID" value="ENSLOCG00000004553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698510"/>
    <dbReference type="KEGG" id="loc:102698510"/>
    <dbReference type="CTD" id="27134"/>
    <dbReference type="eggNOG" id="KOG3580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160036"/>
    <dbReference type="InParanoid" id="W5MAP2"/>
    <dbReference type="OMA" id="VLMRPVK"/>
    <dbReference type="OrthoDB" id="418634at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004553">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090557">
        <property type="term" value="P:establishment of endothelial intestinal barrier"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905605">
        <property type="term" value="P:positive regulation of blood-brain barrier permeability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06727">
        <property type="entry name" value="PDZ1_ZO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06728">
        <property type="entry name" value="PDZ2_ZO1-like_ds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06729">
        <property type="entry name" value="PDZ3_ZO1-like_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000009">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000013">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865:SF11">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN ZO-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="22"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="186"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="685"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="3">
        <location>
            <begin position="937"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1076"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1100"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1308" mass="144398" checksum="63D3C0743168DA71" modified="2014-04-16" version="1">MAVRFKDVNPEMEEMTIWEQYTITVNKDSRMGFGLAISGGRDKPNPMTGDTAVLISDVVRNGPAAGRLQTRDRIVMVNGIAMENVTSSYAIQQLKTCGRTANITVKRPRTIQMPVAAHKAPLSRAASRGTQLSDSGSEGNYYGRSQGRREREARSATPDRNGHALPLMSGMKRLPSQDGPYRPIRATLLKKRATDEYGLKLGSQIFVKHMTPTGLAAKEGTLQEGDLILKINGMATENLSLLDTKHLVERSEGKLSLVVLRDDRQFLVSIPDIEDSEPNSDNDEGSHGDSSGMDDISDLESEPPSSTRRRKISGRKERRTGRNHKPSSTPPVSQDLTPLQVVSLKSSVQAAPLQREPSDTDSENGSLPPPPRLDTPDSRAPRSQYRVFPEAIPAKTRSSPLAPRHDSPQWSSPSSRPSLRGDLESESDRSLSPPSLRDSPSVTSQSSRYRVLPEVTQPAFRASPLALRQDPPRRGHSPGAPPPRGPSDSESDHSSPVPSRWTDTSHSIEPRNRYRLLSDVSQPRSHEPPPSLEPPRWVAPTVQPSLRAPESDSESEVNSSIPPRHDDAPDGRGKYRAFPDVGQPVGPGQDSPPRGHSPIGPPPEDFSDSETESIPSPPRRYESKGYWEQNSRGSIIPPSNGMIRPGVPMEDVSEVHSRASEEPIYSVPPDSPRVRRANSGYSSDLRTVDFVKDGSVGLRLVGGNDVGIFVGGVQPQSQAHQQGMREGDQILQVNGVDFGHFTREEAAMFLLNIRSGEQVVIETQNKMDIYRKIMQSNLGDSFFIRTHFDQVAEGSHGLNFTRGEVFRVVDTMHHGKLGTWLAVRMGNDLHELDKGTIPNYKRAEVIANMEKAQKSSAASSQVPGPRAEFWKLRGLRGAKKNVRRSRDDLLQLTIQAKYPAYEKVLLREASFKRPIAILGPLNDVAMEKLAREMPEEFEVAQMVPRSGGEGSSSVIKLEAVKQISEQDKHPLLDITPTAVERLNYIQYHPLVLFLDPHSRKDVKAMRKRLCPDSKKSSRRLYAQALKTRKYFAHLFSARIELQPGSDMWYEVLKDRICSLQTKPVWVSEVTLESGGEEELDALSRSHSSDYLSCDSRATSDYEDTDGEAYTDGEAYTDEDLDEPYGGREAPRHVGVSLARSSEPAAGEEGPPGFVPDDPRASGRRPPLLHVPEPRSRRQDDPREPSPTSFSEDDPSRFGTPEASAPRPRLESPPNLRASDSLHSLPDPPPHVTPPGGSHPEIQEEASRAVVAEPEEKKPPQPKFVVLAHHQALQWRRSQIQGSDSSDDHAENAVAAADDDDEWGPATEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC46</accession>
    <name>W5MC46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dual specificity tyrosine-phosphorylation-regulated kinase 1A</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein kinase minibrain homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:10217"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01003120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196868.1">
        <property type="nucleotide sequence ID" value="XM_015341382.2"/>
    </dbReference>
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    <sequence length="763" mass="85020" checksum="4B5B5E7E70562CFE" modified="2014-04-16" version="1">MHTGGETSACKPSSVRLAPSFSFHAAGLQMAAPMPHSHQQYSDRHQQSNDPSVTALPYSDQAQQPIANQVTPDVVMLQRRMPQTFRDPASAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPSHILDQAPKARKFFEKLSDSTWGVKKTKDGKREYKPPGSRKLHNILGVEAGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFSAAVQAMECETHCPQVRQPYPPPVGWAGGDGPQQVTVETHPVQETTFHVPPQQPNALHHHTSSNSSHHHHHHHHHHHHGQGLATRNRPRLYNSPTNSASTQDSMEVVHSHLSMTSLSSSASSSSTSSSSTGNQGNQAYQNRPVAANALDFGQNGSMDMGLGVFSNPRQETGITGHPTYQIATNTGPAHYMTEGHLGMRQGIDREESPMAGVCVQQSSVASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCS9</accession>
    <name>W5MCS9_LEPOC</name>
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        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 3</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000005118.1"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_015935_0_0_1"/>
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        <property type="component" value="Linkage group LG17"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
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    </dbReference>
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        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
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    </dbReference>
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        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 3"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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    </dbReference>
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        <property type="entry name" value="DnaJ_domain"/>
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        <property type="entry name" value="J_dom_sf"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LD25575P"/>
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    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="7" id="KW-0834">Unfolded protein response</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="394"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="353"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="504" mass="57772" checksum="4865D8B05DC79D1D" modified="2014-04-16" version="1">MVSVGPFARKVLCYFSYILVLIDLRYEGVQCGKNAGLESHLEMGKKLLAAGQLADALSHFHAAVDGDPKNYLAYYRRATVYLAMGKSKSALPDLSKVIELKPDFTSARLQRGNLLLKQGKLDEAETDFKDVLKSSPSEKEEKEARSQLLKSDEMQRLVAQAKKDYSAKDYKMAAERLDVLIEICVWDVSLRELRANCFIQMGEMGKAISDLKAASKLKNDNTDAFYKLSTIYYKLGDHEMSLNEIRECLKLDPEHKQCFSHYKQVKKLNKQILSAEELIQEQRYADAINKYQSVLKTEADVPLYTFQAKERICHCFSKNQQASEAIAICSEVLSSDPQNLSALKDRAEAYLQDEQYEEAVRDYESARENNENDREISEGLERAQRLLKQSQKRDYYKILGVKRSAQKKEIIKAYRKLALQWHPDNFQDSEEKKKAEKKFIDIASAKEVLTDPEMRRKFDQGEDPLDPENQQSGGSGPHFQRGWNTWTGFNPFGSGPFNFKFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD37</accession>
    <name>W5MD37_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Peroxisomal leader peptide-processing protease</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta-oxidation of fatty acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">The full-lengh TYSND1 is the active the proteolytic processing of PTS1- and PTS2-proteins and in self-cleavage, and intermolecular self-cleavage of TYSND1 down-regulates its protease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase S1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01025164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD37"/>
    <dbReference type="FunCoup" id="W5MD37">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006296"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006304.1">
        <property type="protein sequence ID" value="ENSLOCP00000006296.1"/>
        <property type="gene ID" value="ENSLOCG00000005229.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1320">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014627"/>
    <dbReference type="HOGENOM" id="CLU_034855_1_0_1"/>
    <dbReference type="InParanoid" id="W5MD37"/>
    <dbReference type="OMA" id="CWKSTEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005229">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031998">
        <property type="term" value="P:regulation of fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000080">
        <property type="entry name" value="peroxisomal leader peptide-processing protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="548" mass="59312" checksum="6CB7F1D4409425B2" modified="2014-04-16" version="1">VFKGRMSDMRAVEDWGCTVSVFERGLAEPLSCSGTLLDSRSGIVLCHSLVFSAFLNKSINISDYRVLFPDSFSSRLHVYVQRPEELKSVDNTKQQSHVMRQCLGKAKQTVSKPSPQKAELLMLVSNPQFQKAFTRLFNKPDEWRFCSNTGDIDLDDLKKDTEFLSWFAVLKVPGLLGRGQRALPWVSSSSLKKGDVVFACGSPFGSFCPDLFMNTLSKGIVSNVAGESRALILTDARCLPGTEGGGLFLDKGGRFCLAGLIVSPLCWRAGEWIGLTLVCSLESVVRNIWFVNKGDPCLNWILSDTPSHFVQDLPVTGEAKSVRQHPTVALVQSGELWGSGVLMNSRMMLTCRHVVNGASRVVVKINNKERLLTAVGNVVYSTKDSSPYDVAVVLLAEPLPSTTVPAVARDFRAGRVDVYVTGYGVFGQSCGPSVTSGILSRIIACDFQPAMLQTTCAVQAGASGGAVIRAATGELLGLVSSNTRDCAAGVTYSHLNFSVPVTVLSPLLRQYALTGDSGVFEELDRASDAVRAAWRLQGSHGLIPRSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDJ8</accession>
    <name>W5MDJ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lens fiber membrane intrinsic protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">MP18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">MP19</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">MP20</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Seems to be associated with itself or another lens membrane component via disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDJ8"/>
    <dbReference type="FunCoup" id="W5MDJ8">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006457"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006465.1">
        <property type="protein sequence ID" value="ENSLOCP00000006457.1"/>
        <property type="gene ID" value="ENSLOCG00000005359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSWZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244814"/>
    <dbReference type="HOGENOM" id="CLU_113769_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDJ8"/>
    <dbReference type="OMA" id="KCYMQTE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005359">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000013">
        <property type="entry name" value="lens fiber membrane intrinsic protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003935">
        <property type="entry name" value="LMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF9">
        <property type="entry name" value="LENS FIBER MEMBRANE INTRINSIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01457">
        <property type="entry name" value="LENSMEMPROT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="67"/>
            <end position="88"/>
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        <location>
            <begin position="100"/>
            <end position="121"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="174" mass="19836" checksum="8FE7009D64714FC0" modified="2014-04-16" version="1">RMYSFMGGGLFCAIVGNILLVVSTATDYWMQYRLSGAFAHQGLWRYCMSGKCYMQTDSIAYWNATRAFMILSAMSCFAGIIAGILSFAHFSAFERFNRSFAAGIMFFVSTLFVLLAMAIYTGVTVNFLGKRFGDWRFSWSYILGWVALLMTFFAGIFYMCAYRMHECRRVAGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEB9</accession>
    <name>W5MEB9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">F2R like trypsin receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014687">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006728"/>
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        <property type="gene ID" value="ENSLOCG00000005575.1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="Pro_rcpt_2"/>
    </dbReference>
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        <property type="entry name" value="Protea_act_rcpt"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
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        <property type="entry name" value="PROTEASEAR2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866041" evidence="13">
        <location>
            <begin position="20"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="104"/>
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    </feature>
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            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
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            <end position="237"/>
        </location>
    </feature>
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            <end position="284"/>
        </location>
    </feature>
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            <end position="320"/>
        </location>
    </feature>
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            <begin position="66"/>
            <end position="317"/>
        </location>
    </feature>
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            <end position="200"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="42115" checksum="6F549D73BBD1F075" modified="2014-04-16" version="1">MRLVHLFSLLVICSRQVCSQQRGFLGVETLDGTLVNQTAWDTLQSGLTTVFLPIVYIIVFVVSLPTNAMAIWVFLFRTKKKHPAAIYMANLALSDLLFVIWLPLKIAYHINGNNWIYGEGLCKVLVGFFYANMYCSILFITCLSVQRYWVIVHPLSHQRKNNKIAVYVAITVWAVIWASTTPLYLYDQTVQIKDVDITICHDITKASQINYPFGFFITMGVFAYFIPAVVCIIAYVLMLRHLASSMTDENIGKKRRKAVLLIVTVLVMFLVCFTPSNVMLLVHYSLLKQGEVNNGYGIYIATLCLASLNSCFDPFLYYYVSEEFRENVKNTLICRSVRTVERMRVSFTAMKYSKSSKSYSSDSGTRTSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEM7</accession>
    <name>W5MEM7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Yes1 associated transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the YAP1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015341377.2"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000006844.1">
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        <property type="gene ID" value="ENSLOCG00000005660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687817"/>
    <dbReference type="KEGG" id="loc:102687817"/>
    <dbReference type="CTD" id="10413"/>
    <dbReference type="eggNOG" id="KOG0940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046760"/>
    <dbReference type="HOGENOM" id="CLU_041917_0_1_1"/>
    <dbReference type="InParanoid" id="W5MEM7"/>
    <dbReference type="OMA" id="IIHPRAN"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005660">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:BMP signaling pathway"/>
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        <property type="term" value="P:cell adhesion"/>
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    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
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        <property type="term" value="P:endoderm development"/>
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        <property type="term" value="P:retinal pigment epithelium development"/>
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        <property type="term" value="P:secondary heart field specification"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:ventriculo bulbo valve development"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Putative transcriptional coactivator YAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="transcriptional coactivator YAP1 isoform X2"/>
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    <dbReference type="InterPro" id="IPR053819">
        <property type="entry name" value="TEADIR3_omega_loop"/>
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        <property type="entry name" value="YAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616:SF9">
        <property type="entry name" value="TRANSCRIPTIONAL COACTIVATOR YAP1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616">
        <property type="entry name" value="YES-ASSOCIATED PROTEIN YAP1 FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15238">
        <property type="entry name" value="TEADIR3"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="185"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
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        <location>
            <begin position="293"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="444" mass="48423" checksum="3D31BD2588F1C602" modified="2014-04-16" version="1">MDPSQHNPPAGQPIVHVRGDSETDLEALFNAVMNPKSANVPPSVPMRLRKLPDSFFKQPEPKSHSRQASTDAGTAGTLAPQHVRAHSSPASLLNAVSPGSLAGVIPPGAPPQHLRQASYEIPDDVPLPPGWEMAKTPSGQRYFLNHMDQTTTWQDPRKAMLSQMNAAAPASPMPVQQNIMNSTSGPLPEGWEQAITSDGEIYYINHKNKTTSWLDPRLDPRYALNQQRITQSAPVKQASPLPSQSPQGGVLGGNNQMRLQQLQMEKERLRLKHQELLRQVRPQELALRNQLPTSMEQDGGTQNAVSSPGMAQDVRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDSLGPSSMATQPSRFPDYLDTIPGTNVDLGTLEGDSMAVEGEELMPSLQEALSSDILNDMESVLAATKIDKESFLTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEP7</accession>
    <name>W5MEP7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor Sp7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Zinc finger protein osterix</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with RIOX1; the interaction is direct and inhibits transcription activator activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199537.1">
        <property type="nucleotide sequence ID" value="XM_015344051.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEP7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006856"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006864.1">
        <property type="protein sequence ID" value="ENSLOCP00000006856.1"/>
        <property type="gene ID" value="ENSLOCG00000005679.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682832"/>
    <dbReference type="KEGG" id="loc:102682832"/>
    <dbReference type="CTD" id="121340"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161293"/>
    <dbReference type="HOGENOM" id="CLU_019484_4_2_1"/>
    <dbReference type="InParanoid" id="W5MEP7"/>
    <dbReference type="OMA" id="EYHPFSH"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005679">
        <property type="expression patterns" value="Expressed in zone of skin and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22542">
        <property type="entry name" value="SP7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000077">
        <property type="entry name" value="Sp8 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF19">
        <property type="entry name" value="TRANSCRIPTION FACTOR SP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
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    </evidence>
    <sequence length="1862" mass="202576" checksum="E5A3A3749254A70F" modified="2014-04-16" version="1">DMTRHSSSSSASTPSDCASTPSPLTPPQLSPAGSPECSSPFGPRLPRAKQNFSALRPPPEGASCDGTPDGRLVSIGRAAGKYGRGPCKRGYQPVKMERIKVLTGTEIESDYKEPEILDTRVVMGEEALLRNMEAQGDRVMNKKTEDEGAIPENCPLLPSSAPEEIQERPNEESQLDTGQDSSSVSQEDNPTLQMELAKDPVSLLPSSIQEPIAMDAGMADSDPLMREEGSEKTPSQDEVPSLSFSQPTYMAERQEPDHQLLGSTHSLDPDLYFTAPSTPIKMVYSQHVKQQWFPGSPTPESPPDESPDLPESEGLCSPPTSPSGSYITAEGGSWTSSCTSNTSPSCSPNLITEGELQEAPACYVESLSEIGDELGEERHGADKSEGLGLVENIGFVEGRERLKRETCRPSWVTEDISPQRSSSGRSTTSGEEEGEESEGSLEPAEVLEPTELTADEDLDQDQDLDLDACVAEHFATLNAPLSPEEHRLAAADAGSLTPATCSSEVSDTDNNSPCGEMSILEFPGPCGEGSLEDERMIPASLLPFHASLIFQADSMEITLFPTEDGPGNDVDAYAAGEEEGDVDEGDDEEEEEEEEVEEEGKCVEDVNEEDTSASFLNSLSENSINEGVDESFAFQDDTEESIDSASYNGDEDERLYSTERHAELSQHFPGPDDPAEETQPKEQDSSNSGSGSESESEMEISSGSSDAEHEQKEKCPAPKSSSGASFSSKHPKVQEFIERPEETLTDQPLENVEEVREESETEEQCVDSIITVTEEPADAAYQESLQQSDITIYPQESQMVTERDVLADETTLEAELADQENANSIEYNDLKVSLDSTATNDLNKGVPPLSYPKDDQSSSNIPVSETPEIPTDVPDNLAVTASDVLISESSLDQDNLTENHPCTDEISLDPLYVSSAVYSMLAISPKKENSETNVSGRGTTPELWESGVPVSLEECCEYEAENLLTCSMAGQVINEPVVVPNIETKDTTPADHEANNNCLVNNKNSEFPEDTLLDNSDTGLLESNLSNWKSLEEISEAGGGEDGSSQFPEAQDNPSVPGESALSIVSIFEENPEDTEHKVVKNQSVRGSLQTEDATQDLCEPQPVSSNSLPEEASTSALSLTSETQVNCYPKNVPDLEKSDSTGSPGGGSTVEQNTTLGHIHISKTENVTNNEEGPSSIKSEHAFTLVSGSFGSFIPRQVSSKYKPEEALCPTSPLEIMDSVSENKAKTTESETENSTDEKSDEKTDEKKEDPVEEENTLQSLHHSEQVTCSERERGGEIDKDLDLQEEEEQQKQNADPVDNNHVSEPKSEGAFEENESTDVKIDACISEETITPIENMGSLLTESKQKAEKVTSEQSNSIPFDEQQNATESPDKIKGSDNDKQEIHHEPERSISPIKTTECHRGKEQVAEPPVPTSVQEEEKPSPSISEIPDENMLDVCNAEERKCQIEGMRDINDNHVDDSQNALDEHSETSPLSTSPVNQEPCEELCIPVQESHPIRFPSQLSTKSVSGPGQLSLVPVLDTHTDLQISAHYSQDKKTAQSQMDFLLFSFLDYCSNSVFPHSASSAVDLSVDQLDYCMETPCKSLTQTDSGPGRRVGRQCGSSHTDSSSSSERELLSPCQKNSPVPAPHAAAAEPERFQDRLPRCPMSYSHKVVSNMPSKFKTLSVGSCNDSESDDSVPDLEEPDNHPLQPADTQSQLSPSAPSGDDSMNKAKQSRSEKKARKAMSKLGLRQIHGVTRITIRKSKNILFVITRPDVFKSPASDIYIVFGEAKIEDLSQQVHKAAAEKFKVPLEPSPLITESAPSLSIKEESEDEEEVDETGLEVRDIELVMAQANVSRAKAVRALRHNKNDIVNAIMELTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFI6</accession>
    <name>W5MFI6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFI6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007145"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007153.1">
        <property type="protein sequence ID" value="ENSLOCP00000007145.1"/>
        <property type="gene ID" value="ENSLOCG00000005917.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160463"/>
    <dbReference type="HOGENOM" id="CLU_1513952_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFI6"/>
    <dbReference type="OMA" id="PNDYYFP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005917">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="178" mass="19491" checksum="6A5CD6EF207736B9" modified="2014-04-16" version="1">LSLSLSLPVSLSLPQIVLQTGFWTKYNVGTVVLSLALYFAITFFLHSPRLHQAAPNDYYFPGVSINALSNPLVWLTILLTSVTALLPSFTARALRLVLSSPSMHRVHSSEGPVELQAAFRRGDSHRRSSYAVSQGRGFGQLLTSGARVHNTAPQQDGRETAGVTTSKEMEKLEKPEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFY0</accession>
    <name>W5MFY0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFY0"/>
    <dbReference type="FunCoup" id="W5MFY0">
        <property type="interactions" value="1337"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007289"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007297.1">
        <property type="protein sequence ID" value="ENSLOCP00000007289.1"/>
        <property type="gene ID" value="ENSLOCG00000006037.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155479"/>
    <dbReference type="HOGENOM" id="CLU_141397_1_2_1"/>
    <dbReference type="InParanoid" id="W5MFY0"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006037">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="2">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="107" mass="11690" checksum="F58E5094F8D6D7C1" modified="2014-04-16" version="1">SSLTLAVLSSKVTGIGMDDLNSGVDFASASGLEKAEAAELQRMLAVEQQKAQFQAQETKVHNFTDVCWDKCVDRPGSKLDSRTETCLVNCVERFIDTTLAITSRFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG06</accession>
    <name>W5MG06_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Actin alpha 2, smooth muscle</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630430.1">
        <property type="nucleotide sequence ID" value="XM_006630367.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202174.1">
        <property type="nucleotide sequence ID" value="XM_015346688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG06"/>
    <dbReference type="FunCoup" id="W5MG06">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007315"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007323.1">
        <property type="protein sequence ID" value="ENSLOCP00000007315.1"/>
        <property type="gene ID" value="ENSLOCG00000006057.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682501"/>
    <dbReference type="KEGG" id="loc:102682501"/>
    <dbReference type="CTD" id="59"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154148"/>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="W5MG06"/>
    <dbReference type="OMA" id="PXEREIV"/>
    <dbReference type="OrthoDB" id="6953074at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006057">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090131">
        <property type="term" value="P:mesenchyme migration"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0558">Oxidation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="377" mass="42023" checksum="5A7EB6FD485AEB09" modified="2014-04-16" version="1">MCDEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGM9</accession>
    <name>W5MGM9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cathepsin Z</fullName>
            <ecNumber evidence="3">3.4.18.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity. Capable of producing kinin potentiating peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of C-terminal amino acid residues with broad specificity, but lacks action on C-terminal proline. Shows weak endopeptidase activity.</text>
            <dbReference type="EC" id="3.4.18.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.18.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01024342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGM9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007538"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007546.1">
        <property type="protein sequence ID" value="ENSLOCP00000007538.1"/>
        <property type="gene ID" value="ENSLOCG00000006242.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155569"/>
    <dbReference type="HOGENOM" id="CLU_012184_2_1_1"/>
    <dbReference type="InParanoid" id="W5MGM9"/>
    <dbReference type="OMA" id="QSWDWRN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006242">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02698">
        <property type="entry name" value="Peptidase_C1A_CathepsinX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000060">
        <property type="entry name" value="Cathepsin Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033157">
        <property type="entry name" value="CTSZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018770048" description="Cathepsin Z" evidence="13">
        <location>
            <begin position="29"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="14">
        <location>
            <begin position="65"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="34513" checksum="FDC5C93448E03834" modified="2014-04-16" version="1">TSSYPTELRLAMAGQLFLALCLCPAVLAGLHFNEKQPCYRPMSRGDLPGVQSGPRPHQYLSLSDLPKAWDWRNVNGTNYVSTTRNQHIPQYCGSCWAHGSTSALADRINIKRKGAWPSAYLSVQNVIDCGDAGSCEGGDHSGVWQYAHTHGIPDETCNNYQAKDQDCKPFNQCGTCTTFGVCNVIKNYTVWKVGDYGPVSGREKMMAEIYARGPISCGIMATAKLDAYTGGLYSEYNPQPYINHIVSVAGWGLDENGAEFWIVRNSWGEPWGEMGWLRIVTSAYKGGSGSQYNLAVEEDCYYGDPMLPKHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH88</accession>
    <name>W5MH88_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TNF receptor-associated factor</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TNF receptor-associated factor family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01035495">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640851.1">
        <property type="nucleotide sequence ID" value="XM_006640788.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222732.1">
        <property type="nucleotide sequence ID" value="XM_015367246.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222733.1">
        <property type="nucleotide sequence ID" value="XM_015367247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH88"/>
    <dbReference type="FunCoup" id="W5MH88">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007747"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007756.1">
        <property type="protein sequence ID" value="ENSLOCP00000007747.1"/>
        <property type="gene ID" value="ENSLOCG00000006409.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684289"/>
    <dbReference type="KEGG" id="loc:102684289"/>
    <dbReference type="eggNOG" id="KOG0297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161076"/>
    <dbReference type="HOGENOM" id="CLU_021061_4_1_1"/>
    <dbReference type="InParanoid" id="W5MH88"/>
    <dbReference type="OMA" id="CIVETNA"/>
    <dbReference type="OrthoDB" id="6499288at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006409">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="41"/>
            <end position="81"/>
        </location>
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        <location>
            <begin position="133"/>
            <end position="185"/>
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        <location>
            <begin position="410"/>
            <end position="556"/>
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        <location>
            <begin position="186"/>
            <end position="217"/>
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        <location>
            <begin position="317"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
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            <begin position="194"/>
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        </location>
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            <dbReference type="PROSITE" id="PS50089"/>
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            <dbReference type="PROSITE" id="PS50145"/>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="560" mass="62788" checksum="EE54EEAA155780C3" modified="2014-04-16" version="1">MAGKSTGDLYNGLSSAPGESEYPSGFPQNICDDVPQEKYLCSNCNNLLNKARQTLCGHRYCLACVNWLMRNNMNLVCKKCKEEDPNLDNENSILIPERFFNDAAINKEISELKVHCANQGCNWRSTLKNYEDHQSQCDYALIPCNIGCGHMVIRKNLASHLEKGCAKNMLVCQTCSHKMSQAEFQKHTCQSSPAKERKPSKTETREKNRQLSSAKNKDPCRFSEVGCTFKGTKEKVKEHESTGLVAHLQLLLQVISNLKVSCLTTISKGDGGKAHVSGTEFKLTELEESLASMQMQGAVEVNGDLETDGYTEPSISQGAQKEQLGEFKQKLSELQQKIYIYENIVTVLNREVEKNQAAVLALEHQNKNSQDNIANLELQVAEQQGKLAVKDLLISSLHLRISALEEASHDGTFIWKISNLSKKMQEAAAGRENNLYSPAFYTGKYGYKVCMRCYLNGDGMGKGTHISLFFVVMKGEYDALLSWPFKHKVTFFLLDQNQREHVIDAFRPDLSSTSFQRPVRDMNVASGCPLFFPHAKLKSPKHAYSKDDTLFIKCIVDTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHD8</accession>
    <name>W5MHD8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cilia and flagella associated protein 144</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000007797"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007806.1">
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        <property type="gene ID" value="ENSLOCG00000006457.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_155974_0_0_1"/>
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        <property type="component" value="Linkage group LG10"/>
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        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
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        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN FAM183B"/>
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        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="143" mass="16635" checksum="D07E701621AF6868" modified="2014-04-16" version="1">EARSRMAAKGKEKEKEPVDIVHQNAILCETIMKEQRSQKLYTQFSINPFKKLHVLPGKPMPRHANEDGKEDPNFLKIIHGARLEPTKKYTHPQTESQEIGWISTPLIVSDRSDRRINFPRQNSEITKYMDAAWRLKEQTQNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI37</accession>
    <name>W5MI37_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Single Ig and TIR domain containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SIGIRR</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI37"/>
    <dbReference type="FunCoup" id="W5MI37">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008046"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008056.1">
        <property type="protein sequence ID" value="ENSLOCP00000008046.1"/>
        <property type="gene ID" value="ENSLOCG00000006662.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="HOGENOM" id="CLU_040046_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI37"/>
    <dbReference type="OMA" id="WCTNNFR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006662">
        <property type="expression patterns" value="Expressed in intestine and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF19">
        <property type="entry name" value="SINGLE IG IL-1-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="18"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="5">
        <location>
            <begin position="174"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="367" mass="41835" checksum="8F69C6AA3131C643" modified="2025-10-08" version="2">MTLLSVSVCPDNVCSGAPEFLSPLGTPVLTSTLGAQLSLNCTARLSSDPQDPHCDSALHWLKDDQLLTNTSLYPRNDSEWSANNGSQRFVRSLLQVTVRDQGSYGVFSCRLSNATATFKLQRTGKELEHTSDWAMELLLFLSCSTGAVRYSRCRLNLRLWYKNAYGDYEMNDGKLYDAYVSYVNNEHDRKFVNFILKPHLENRYSYKLHLHDGDILPGSEPSAELLMNVSRCRRLIVVLSRAYLEQEWCTNSFREGLWRLQELCRRPVFIVFESQRRQLSPPALRLLKESGRSVALLLWGSKSMTPSSQFWKSLVLEMPRKLMYHHNTGDPQTLLQDDKDPMLTLNPDYLDCRPDPDPAGDLGELGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIB7</accession>
    <name>W5MIB7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01018093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIB7"/>
    <dbReference type="FunCoup" id="W5MIB7">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008126"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008136.1">
        <property type="protein sequence ID" value="ENSLOCP00000008126.1"/>
        <property type="gene ID" value="ENSLOCG00000006730.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155985"/>
    <dbReference type="HOGENOM" id="CLU_030988_14_4_1"/>
    <dbReference type="InParanoid" id="W5MIB7"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006730">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23793">
        <property type="entry name" value="UBCc_UBE2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000003">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="55"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="22375" checksum="57E95CBB0966AA4A" modified="2014-04-16" version="1">MSSEVQRPDDSPSTSGGSSDIDQRESVHQEQEREQTQPKKKETKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRIARQWTKRYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIF5</accession>
    <name>W5MIF5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protease serine 4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01038519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIF5"/>
    <dbReference type="FunCoup" id="W5MIF5">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008164"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008174.1">
        <property type="protein sequence ID" value="ENSLOCP00000008164.1"/>
        <property type="gene ID" value="ENSLOCG00000006753.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696352"/>
    <dbReference type="KEGG" id="loc:102696352"/>
    <dbReference type="CTD" id="777630"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159163"/>
    <dbReference type="HOGENOM" id="CLU_006842_19_2_1"/>
    <dbReference type="InParanoid" id="W5MIF5"/>
    <dbReference type="OMA" id="GNWASAC"/>
    <dbReference type="OrthoDB" id="6380398at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006753">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="231"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="50525" checksum="95191EEC86BBEC7D" modified="2014-04-16" version="1">MKQKKAAQQPPVEVRQPLNPANSGEAKPRRVRAQMTASSPQQEKARRRRGIITALIVLTVLGLLATGGYFVKRLVDSKYLFCKTSFKFIPLSSACNGIEDCAGGEDELSCVSNLTVTVIFPVRLVSNRSLLQVYDSRSDSWRLVCAEGWQPKHTQQACQQLGYTSNPSSSLEPVESVPLTVNQAFGGLMTSDPNGAFLIQDLVTDRTVCSSGMVISLTCSDCGERSSEDRIVGGSDATIQEWPWQVSLQFNEQHTCGGSLIAPRWVVTAAHCFPRAEHVLSRWSVHPGLTSLGFTGGVSVDKVIVNGQYNSYISDYDIAMMRLSSPVQVSATSRPVCLPAFNQELPSQAPLWVTGWGYRVEKGEVSTVLQQATVPLIDRARCSQSSVYGSAITPRMLCAGYLEGKVDACQGDSGGPLVYFQSSWELVGVVSWGVGCARQNRPGVYSNVQSFLNWIHVVMEKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ17</accession>
    <name>W5MJ17_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">P2X purinoceptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ17"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008376"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008386.1">
        <property type="protein sequence ID" value="ENSLOCP00000008376.1"/>
        <property type="gene ID" value="ENSLOCG00000006925.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSBN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230351"/>
    <dbReference type="HOGENOM" id="CLU_034469_7_0_1"/>
    <dbReference type="InParanoid" id="W5MJ17"/>
    <dbReference type="OMA" id="YYCSPEY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006925">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003050">
        <property type="entry name" value="P2X7_purinoceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF13">
        <property type="entry name" value="P2X PURINOCEPTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01314">
        <property type="entry name" value="P2X7RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="15">
        <location>
            <begin position="385"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="581" mass="66588" checksum="01AEBA5406F04F87" modified="2014-04-16" version="1">MPCSLLNFCEYETNKLVRIKSIPIGSLKWSINGLILGFICIMLFWNKEYQEYDQVVSSVTAKVKGVAVTNITDVGEILWDAADYSGLSQGKNSFFVMTNAIVTNKQRQGKCAEVPGSGKICYRDQDCTKGYSDQHSHGVQTGSCVKYDTTRKTCQVVAWCPIESKNKPPKPALLESAENFTVLIKNNIRFPAFNYIKRNILPHMNDTYLKSCMFSRRMDPLCPILRLGDIVQEARENFSQMAVEGGVIGIQINWHCNLDRFFHKCLPEYSFRRLDEKESNKTLYPGLNFRFARYNMNNGIEERMLFKAFGIRFDVMVFGKAGRFSIIQLVIYIGSTLSYYALTTVFIDWFITTYLYPRCCARATKNNYSEKKYETVEELNQIPLCISFVDEDQIIVVKRPLKKSLQETKPSSSQHRKPEHSFGRTRVLVNLLQSPSRNADTEIQEMQPPKGRGRSAPAWCQCGRCQPMPTPQEQLCCRRSKGQCITTSPAFPRLVLDRHTLESALLYREPLLELPQARCHGHLRHCAYSLYIEWRFGRIQTGTFAAVPSCSVWRIRDEYPSEDGRYSGLCYENMVIISSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ50</accession>
    <name>W5MJ50_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane protein 201</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM201 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ50"/>
    <dbReference type="FunCoup" id="W5MJ50">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008409"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008419.1">
        <property type="protein sequence ID" value="ENSLOCP00000008409.1"/>
        <property type="gene ID" value="ENSLOCG00000006950.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4623">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164281"/>
    <dbReference type="InParanoid" id="W5MJ50"/>
    <dbReference type="OMA" id="LCLGTMP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006950">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005521">
        <property type="term" value="F:lamin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018617">
        <property type="entry name" value="Ima1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040041">
        <property type="entry name" value="TMEM201"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018861">
        <property type="entry name" value="TMEM201_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28646">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28646:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10476">
        <property type="entry name" value="DUF2448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09779">
        <property type="entry name" value="Ima1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Ima1 N-terminal" evidence="9">
        <location>
            <begin position="45"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 201 C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="658" mass="71059" checksum="E1D6D3BDAAFD2C00" modified="2014-04-16" version="1">MEVFGQMLAEYPQLTLAGGVGATACAAGAVVYRIATRKKPTHVQVNCWFCSQDSVVPYGNRNCWDCPNCEQYNGFQENGDYNKPIPAQYMEHLNHGVSAGLPVPQTPKTLQFVNCQMLLCKKCNNNQTLKIKQLASFAPREEEKYDEEIEVYRHHIEQTYKLCRPCQTAVEYYIKHQNRQLRALLFSHQLRRGRDADKAFVQNSYSFSTPTRVILLRVLAFLACAFLVALVTCGSRDPFLTAGAPEAPVRPLASNASAPGNSSAGSTGIWPELLSLFPREVLETLQAAWDYGRSHQLAVVCTGLLACIMAVFMAGRIRLRRIDAVASVLWLLVMGVHVLERYLETGAPRWLDAVKLGTTSLCCLVGFAAAVATRKSSGQRRSRARRYLSGGSVHPLYGGDASLLSPALSGSSAFVPTPPPNLSRLISHQLAHRARRPSPSSLPGRLNRALSLGTIPSLTRTDSGYLFSGSRPSSQCKDSSPSDYYSLKPGSRPASPGPSPTPSVAGSVTSSSGSARQRRPLISPARLQLAGRRLQLFSSPAAPCAAFAPEVPAFPPHQQGTSAVHSSLQSRQRCPFTVLADARSVLEGGSVFGDAEQGKRDSSSGSSTCLVDTTTGGGADSGAGWKGLLGRSLWQGMLGVSLTLNIVLTGAYVYHSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK55</accession>
    <name>W5MK55_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK55"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008774.1">
        <property type="protein sequence ID" value="ENSLOCP00000008764.1"/>
        <property type="gene ID" value="ENSLOCG00000007244.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161975"/>
    <dbReference type="HOGENOM" id="CLU_357128_0_0_1"/>
    <dbReference type="InParanoid" id="W5MK55"/>
    <dbReference type="OMA" id="ISCAMCA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007244">
        <property type="expression patterns" value="Expressed in testis"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000169">
        <property type="entry name" value="TLR4 interactor with leucine rich repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF5">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="522"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="641" mass="71464" checksum="59BB700D9207E026" modified="2014-04-16" version="1">NITLNGKCQMKFNLRGFLKIQASLCPEACNCNDMDVFCSNSGLTYVPQILGTHSPAQYLSLGGNFIRSVGTGDFLFYTQLNRLDLQYNVISSIQPRSFESLKKLKELYLGHNRISFLVNQSFSGLSNLLYLDLNKNQLSSVQRDAFNGLVNLRKLRLSGNDVSYLPSGLFSDLKMLELLYLEDNRISSLSRNVFKGLGNLRELYLSRNNVTILSGDVFKHLRSLSDIQLDDNAITRIWNGNFLTLSELKTLSLSNNVITEIDSGAFNGLSKLKVLNVDGNRISAIGKQFLSRSLHLEEIDLSGNQIGTVEESALDGLRFLTVLKLNNNNLRYLNPGVFKTNLQLSILDLHDNPWHCNCGIIDLKNWLKSSSDRILTTFVKCSIPEKLKGMYLDFVSSEVIQSADPACPDTMLSDEPGTFMTGNRLATQRDTEEEKETHTRATATRVFTMPDSFTTVIKNTTKRHITSAESGKPQSLTTQATSSYGNRSMSQNTATPTLVTTTAFNKPSHLKNITPCDYNQNAILNISARVVTSNSAEISWNLARAGKEHVYFRIMYDQFDGNIRFSRFINVNKGNVCNLRDLRASTSYFVCVETVVNEKVCEVAPRDLCIGIVTKGEENSTPDPQLVILYISGANAVAITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML56</accession>
    <name>W5ML56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centromere protein H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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    </evidence>
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        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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    <comment type="similarity">
        <text evidence="1">Belongs to the SZRD1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="SUZ-C"/>
    </dbReference>
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        <property type="entry name" value="SZRD1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="5">
        <location>
            <begin position="106"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="159" mass="17582" checksum="092B2465A27CBAA1" modified="2014-04-16" version="1">MEDEEVAESWEEAADSGEIDRRLEEKLKISQKEKKSSRSPLKTAIVIQDDSLPAAPPPQIRILKRPSSNGSLGASQAAQRPLPQVKSLAQREAEYAEARRRILGSASPEDEPERALASVVHTERLVRVSAGPPEESRPHNHVIRQPAGPDGTQGFRQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMJ1</accession>
    <name>W5MMJ1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Family with sequence similarity 234 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMJ1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009611.1">
        <property type="protein sequence ID" value="ENSLOCP00000009600.1"/>
        <property type="gene ID" value="ENSLOCG00000007887.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R0CE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063694"/>
    <dbReference type="HOGENOM" id="CLU_036490_0_0_1"/>
    <dbReference type="InParanoid" id="W5MMJ1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007887">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="8">
        <location>
            <begin position="79"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="549" mass="58820" checksum="55BB38F6F1ACC6DE" modified="2014-04-16" version="1">MSDSASSVAEAQPLKAEEREGPPAPQKKQGGCRAGSGLSRLSHWRTAAFFLSLFLCLAIVFAFSFIIPCPVRAVSLRTWNRTFADAGAYNFLAIMDVNRDKVMDVVFAYKDSTGSLNTTCSDKNLSSPCVVLAAASGTNGSPLWERPLEAELRWAQCGVEGLGGLDTAGCLVSHSSFLTAVDQHRGQVLWTVPGPPGLDLQLPVLTAPDLDGDAVQDVVLIGVTQNMTKAIILSGKTGSGMGAEVVLEQEAVSGHLLHTTERGAPYVLLQTGSGVQGYALRTIAAQAEEGSETRLKTDSQWEAKAGPGGRVPVDRSSSVRYLTKAAQRSGVPHLLVVTAEHTRMIHGQSLETQWTVNASGVLSEPSFGYFSRDSRDETPDVVIEADVGHGMKKVLILDGSSGAVLWEETMVHRAGSPRPASVNTLSYNSVFVFWGAAARGNGTSASPKERFMYMLHPSQPDALLEKSSTPDPIAAFKAALFEKGRHACLVLLTGPEGQGAAGEVTLAKRKLKDDVPESRVLWLRGGGGPSRDQEVRDFFNNLRFKRSTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMX8</accession>
    <name>W5MMX8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Abraxas 2, BRISC complex subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the FAM175 family. Abro1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMX8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009737"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009748.1">
        <property type="protein sequence ID" value="ENSLOCP00000009737.1"/>
        <property type="gene ID" value="ENSLOCG00000008016.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTRN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063424"/>
    <dbReference type="HOGENOM" id="CLU_040659_0_0_1"/>
    <dbReference type="InParanoid" id="W5MMX8"/>
    <dbReference type="OMA" id="HHDPCAQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008016">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23524">
        <property type="entry name" value="Abraxas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023240">
        <property type="entry name" value="BRISC_Abraxas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023238">
        <property type="entry name" value="FAM175"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31728">
        <property type="entry name" value="ABRAXAS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31728:SF1">
        <property type="entry name" value="BRISC COMPLEX SUBUNIT ABRAXAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21125">
        <property type="entry name" value="MPN_2A_DUB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02053">
        <property type="entry name" value="BRISCABRO1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02051">
        <property type="entry name" value="PROTEINF175"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MPN" evidence="14">
        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="264"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="444" mass="48994" checksum="841899C3ACF20C89" modified="2014-04-16" version="1">MSCGIGGDVSIMAASISGYTFSSVCYHSANSNSDHEGFLLGEVRQEETFSISDSQISNTEFLQVVEVHNHEPCAHLFSFYDYAGKVNEENLNRILKDRRKNVIGWYRFRRNTQQQMSFREQIIHKQLTRILGVPDLVFLLFSFISTANNSTHALEYVLFRPNRSRYNQRVSLTIPNLGNTSQQEYKVSSVPNTSWNYAKVIKEHGAEFFDKDGVMKDIRTIFQVYSALQEKVQAVCAEVEQSERVKENIQEEVNKLKQHIALRKRKNAEEERRVREAAAGPAAVGGQAPVEDSESPLHSREAFPTAASDRPDPEEPLSSDVPPPYAEVVPRDSPAVPCPEPSTALLPRPQAVGSSGHVPPGLALGGAGGAEYSGNGDGSSTDSSDRRAAGQEDEEDSSSEYENLVQEPSESSDNEVPPLPQARPAALRPDGDARSSQASPIRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN67</accession>
    <name>W5MN67_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the subunit alpha (ATP5F1A), forms the catalytic core in the F(1) domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:57722"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01020077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN67"/>
    <dbReference type="FunCoup" id="W5MN67">
        <property type="interactions" value="1185"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009826"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009837.1">
        <property type="protein sequence ID" value="ENSLOCP00000009826.1"/>
        <property type="gene ID" value="ENSLOCG00000008090.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074800"/>
    <dbReference type="HOGENOM" id="CLU_022398_0_1_1"/>
    <dbReference type="InParanoid" id="W5MN67"/>
    <dbReference type="OMA" id="SMEEGGW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008090">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="197"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="527" mass="56223" checksum="A3FB565B6F6B2EFB" modified="2014-04-16" version="1">PSAILAMLGAVGRCCTGALQALKPGVNPLKVFSGTPAALYSRRNYAAQASPSAKPVTANGRIVAVIGAVVDVQFDEGLPPILNALEVAGRDTRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVIDTGAPIRIPVGPETLGRIMNVIGEPIDERGPISTKQTAPIHAEAPEFTDMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDTTSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGAEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFQVAEVFTGHLGKLVPLKETIKGFKSILAGEYDGLPEQAFYMVGPIEEVVQKAEKLAEEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNF1</accession>
    <name>W5MNF1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CNN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634943.1">
        <property type="nucleotide sequence ID" value="XM_006634880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNF1"/>
    <dbReference type="FunCoup" id="W5MNF1">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009910"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009922.1">
        <property type="protein sequence ID" value="ENSLOCP00000009910.1"/>
        <property type="gene ID" value="ENSLOCG00000008165.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686219"/>
    <dbReference type="KEGG" id="loc:102686219"/>
    <dbReference type="CTD" id="326931"/>
    <dbReference type="eggNOG" id="KOG2046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154539"/>
    <dbReference type="HOGENOM" id="CLU_055232_0_1_1"/>
    <dbReference type="InParanoid" id="W5MNF1"/>
    <dbReference type="OMA" id="DYPLEYQ"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008165">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000040">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF17">
        <property type="entry name" value="CALPONIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="36250" checksum="80409C471786F17F" modified="2014-04-16" version="1">MTQFNKGPAYGLSAEVKSKIAQKYDSQKEEELRYWIEDVTGMPIGDNFQKGLKDGVILCELINKIQPGSVKKINHSKLNWHKLENLGNFIKAILTYGLKPNDIFEANDLFENGNMTQVQTTLLALASMAKTKGIGSKVDIGVKYADKQTRQFDEEKLKAGQCVIGLQMGTNKCASQAGMTAYGTRRHLYDPKTQADRPYDQTTISLQMGTNKGASQAGMAAPGTRRDIFDQKVAQQPVDCSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCASPTEPVIHTNGSQGTGTNGSEISDSDYQAEYPEEYQGNYNDEYHGHYNDQGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPH9</accession>
    <name>W5MPH9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ring finger protein 175</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPH9"/>
    <dbReference type="FunCoup" id="W5MPH9">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010288"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010301.1">
        <property type="protein sequence ID" value="ENSLOCP00000010288.1"/>
        <property type="gene ID" value="ENSLOCG00000008446.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1734">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013075"/>
    <dbReference type="InParanoid" id="W5MPH9"/>
    <dbReference type="OMA" id="PYWERTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008446">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16475">
        <property type="entry name" value="RING-H2_RNF121-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000074">
        <property type="entry name" value="Ring finger protein 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040176">
        <property type="entry name" value="RNF121/RNF175"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407:SF2">
        <property type="entry name" value="RING FINGER PROTEIN 175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407">
        <property type="entry name" value="RNF121 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="331" mass="38661" checksum="9E4F91D783A7C3D6" modified="2014-04-16" version="1">LSRRVMAAVAAQVVEIDSLNEQRDLLKMSPEDNWKMEHEKMHIKHRGHEAMHAEMVLILIATLVVAQIVLVQWKQRHCRSYNMVTLLQMWVVPLYFTLKLYWWRFLSTWGMFSVITSYVIFRATRKPLSGRTPRMVYKWFLLIYKLSYAVGIVGYVAIMFTMFGFNLFFRIKTEDSMDFGVILLFYGLYYGVMGRDFAEICSDYMASTIGYYNVGAMPSRNLSGDICAVCGQKIFVNLDEEGIVENTYQLSCGHIFHEFCIRGWCIVGKKQTCPYCNEKVDLKKMLNNPYKSWERTHVLYGQLLDWLRYLVAWQPVIIGVVHGINYTLGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSF4</accession>
    <name>W5MSF4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">trans-L-3-hydroxyproline dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.77</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>trans-3-hydroxy-L-proline = 1-pyrroline-2-carboxylate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10320"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:39785"/>
            <dbReference type="ChEBI" id="CHEBI:57938"/>
            <dbReference type="EC" id="4.2.1.77"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proline racemase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.77" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01003879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSF4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011313"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011329.1">
        <property type="protein sequence ID" value="ENSLOCP00000011313.1"/>
        <property type="gene ID" value="ENSLOCG00000009274.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002032"/>
    <dbReference type="HOGENOM" id="CLU_036729_0_1_1"/>
    <dbReference type="InParanoid" id="W5MSF4"/>
    <dbReference type="OMA" id="SHVLWTG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009274">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050346">
        <property type="term" value="F:trans-L-3-hydroxyproline dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.10:FF:000007">
        <property type="entry name" value="Trans-L-3-hydroxyproline dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008794">
        <property type="entry name" value="Pro_racemase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33442">
        <property type="entry name" value="TRANS-3-HYDROXY-L-PROLINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33442:SF1">
        <property type="entry name" value="TRANS-3-HYDROXY-L-PROLINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05544">
        <property type="entry name" value="Pro_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029792">
        <property type="entry name" value="Pro_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00028">
        <property type="entry name" value="Proline_Racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="348" mass="37583" checksum="5110E3C0FECF295F" modified="2014-04-16" version="1">FADMDGVKIPPHDGPILSVVDMHTGGEPLRVIVSGYPEVKGESVLAKRRYVRENLDHLRRVLMFEPRGHYDMYGALVVQSELSDAHLGVLFMHNEGYSTMCGHAVIALGRFAVDYGLIEAPQSPETQVNIHCPCGLVKAFVEYCNGRAGKVRFHSVPAFAFAIDVAVSVPGYGEVLVDIGYGGAFYAIVSAETFGVDVKTSRTRDLVDIATAVSNAVKSQVELHHPTSPDLAFLYGTILTDGKDTFSQEPTANVCVFADAQVDRSPTGSGVTARIALQYYKGLIQLNETRTFQSGATGSLFTGRAVEETKCGDFKAVVVEVAGHAHYTGIASFVLENDDSLKDGFLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTB4</accession>
    <name>W5MTB4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Corticotropin releasing hormone receptor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010306">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209811.1">
        <property type="nucleotide sequence ID" value="XM_015354325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTB4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011623"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011640.1">
        <property type="protein sequence ID" value="ENSLOCP00000011623.1"/>
        <property type="gene ID" value="ENSLOCG00000009503.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683391"/>
    <dbReference type="eggNOG" id="KOG4564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156795"/>
    <dbReference type="HOGENOM" id="CLU_002753_4_1_1"/>
    <dbReference type="InParanoid" id="W5MTB4"/>
    <dbReference type="OMA" id="RINYSHC"/>
    <dbReference type="OrthoDB" id="6022368at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009503">
        <property type="expression patterns" value="Expressed in heart and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015056">
        <property type="term" value="F:corticotrophin-releasing factor receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043404">
        <property type="term" value="F:corticotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060291">
        <property type="term" value="P:long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15446">
        <property type="entry name" value="7tmB1_CRF-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000021">
        <property type="entry name" value="Corticotropin releasing factor receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000025">
        <property type="entry name" value="Corticotropin releasing hormone receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003053">
        <property type="entry name" value="GPCR_2_CRF2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003051">
        <property type="entry name" value="GPCR_2_CRF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF19">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01279">
        <property type="entry name" value="CRFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01281">
        <property type="entry name" value="CRFRECEPTOR2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868254" evidence="14">
        <location>
            <begin position="20"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="15">
        <location>
            <begin position="34"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="16">
        <location>
            <begin position="124"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="419" mass="49070" checksum="EE2D9DB12FF71E8A" modified="2014-04-16" version="1">MDLRKIIFSLFVLYPVIHCEVSEHLSLFILLKKYCEALSSFSNVTGLYCNATTDEIGTCWPKSSTGRIVERPCPEYINGVKYNTTRNAYRECLENGTWAFKSNYSNCEAILEEKRKYPMHYKIALIINYLGHCISVGALIVAFMLFLCLRSIRCLRNIIHWNLITTFILRNVMWFLLQLIDHNIHESNEPWCRLITTIYNYFVVTNFFWMFVEGCYLHTAIVMTYSTDKLRKWVFLFIGWCIPCPIIVAWAIGKLYNENEQCWFGKEPGKYIDYIYQGPVILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDISQIVFIYFNSFLQSFQGFFVSVFYCFLNGEVRSAARKRWHRWQDNHALRVRVARAMSIPTSPTRISFHSIKQTTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTV7</accession>
    <name>W5MTV7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTV7"/>
    <dbReference type="FunCoup" id="W5MTV7">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011816"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011834.1">
        <property type="protein sequence ID" value="ENSLOCP00000011816.1"/>
        <property type="gene ID" value="ENSLOCG00000009671.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260040"/>
    <dbReference type="HOGENOM" id="CLU_058290_1_0_1"/>
    <dbReference type="InParanoid" id="W5MTV7"/>
    <dbReference type="OMA" id="YDEMVNE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009671">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
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        <property type="entry name" value="14-3-3_CS"/>
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    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="6">
        <location>
            <begin position="14"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="255" mass="28815" checksum="584AF9EFC81DD2C2" modified="2014-04-16" version="1">SCLLPPQPQAAMDKSELVQKAKLAEQAERYDDMAASMKAVTEQGGELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGSDKKQQMAKEYREKIEEELKEICKDVLALLDKFLIPNASHAESKVFYLKMKGDYYRYLAEVAVGEEKNTIISNSQQAYQAAFDISKKDMQPTHPIRLGLALNFSVFYYEILNSPEQACSLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDNQGEEEAEDGKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV38</accession>
    <name>W5MV38_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prostaglandin E2 receptor EP3 subtype</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Prostanoid EP3 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts (via C-terminus) with MKLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV38"/>
    <dbReference type="FunCoup" id="W5MV38">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012247"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012268.1">
        <property type="protein sequence ID" value="ENSLOCP00000012247.1"/>
        <property type="gene ID" value="ENSLOCG00000010015.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234559"/>
    <dbReference type="HOGENOM" id="CLU_045991_3_0_1"/>
    <dbReference type="InParanoid" id="W5MV38"/>
    <dbReference type="OMA" id="HMKTRVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010015">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014827">
        <property type="term" value="P:intestine smooth muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060836">
        <property type="term" value="P:lymphatic endothelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060455">
        <property type="term" value="P:negative regulation of gastric acid secretion"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903672">
        <property type="term" value="P:positive regulation of sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000087">
        <property type="entry name" value="prostaglandin E2 receptor EP3 subtype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001244">
        <property type="entry name" value="Prostglndn_DP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000265">
        <property type="entry name" value="Prostglndn_EP3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF10">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP3 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00428">
        <property type="entry name" value="PROSTAGLNDNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00582">
        <property type="entry name" value="PRSTNOIDEP3R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="58"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="41716" checksum="1D30B3A96B23704C" modified="2014-04-16" version="1">MTSDGCDSVDTLGNSSSGAMWDPNISKTMREVNGSKNPECGSVSVAFPITMMITGMVGNSLAIMLVYISYRKRENKKKKSFLLCIGSLALTDLFGQLLTSPIVISVYRADLKWYRVDPSGRLCAFFGVCMTMFGLCPLFLASAMAVERTLAITSPHWYSNNMKTSFTKLALAFIWCSVFLFALLPIAGVGQYTLQWPGTWCFISTGDSNVAGNTFFSITFAVLGIFSLIVTFSCNVVTIKALVSRCKTKPASSQSGKQWERLTVETVIQLMGIMCVLFTCWSPLLILMLKMISTHTSSHQCKTAAFSLSPSAEIQAVCNFYLTAIRLASLNQILDPWVYLLLREILFRKFCQVANAVSSCSIDGPKETQAALDALNKQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVC2</accession>
    <name>W5MVC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leiomodin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Myofibril</location>
            <location evidence="6">Sarcomere</location>
            <location evidence="6">M line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVC2"/>
    <dbReference type="FunCoup" id="W5MVC2">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012331"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012352.1">
        <property type="protein sequence ID" value="ENSLOCP00000012331.1"/>
        <property type="gene ID" value="ENSLOCG00000010083.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685319"/>
    <dbReference type="KEGG" id="loc:102685319"/>
    <dbReference type="CTD" id="56203"/>
    <dbReference type="eggNOG" id="KOG3735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159731"/>
    <dbReference type="InParanoid" id="W5MVC2"/>
    <dbReference type="OMA" id="GMWERLG"/>
    <dbReference type="OrthoDB" id="2163268at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010083">
        <property type="expression patterns" value="Expressed in muscle tissue and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000078">
        <property type="entry name" value="Leiomodin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF3">
        <property type="entry name" value="LEIOMODIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="587" mass="67066" checksum="494B515117CDEFC6" modified="2014-04-16" version="1">MSDRNQNSDQESYGGEIDEDEILANLSPEELKQLQNEMEVIAPDEEVPVGMRQKDQTEKPPTGSFDHRSLVDYLYWQKESNRMLEEERVPTTLLPSKRNTREDHDNEGVADKKEIECYKEYVKVCDKEEDVNDIHKDMQRCSVKPGGTSACVTNKAQENMKSKKPTARSSECPVVGRKTEDQCFKTKEKRSEISKSVQTNGLHSNSEISAPETKSMKKSSEETGTADEVPEKEEKKISKLKIPNKLTAGGNFIKLTARPSGNETNLEKTLEKIRKNSPEIKDVNLNNIENIPKEMLLDYVDALKKNKHVVTFSIANTGADENVAFALANMMRENRSITTLNIESNFITGKGIIAIMRCLQFNETLTELRFHNQRHMLGHHAEMEMSRLLKANNTLLKMGYHFELPGPRMVVTNLLTRNLDRQRQKRMEEQRQQQMKEQEQTMSLFQNSLEIPPGLWEMLGGYLPAAPPTGASPDPPQAPLPLLQMKRRRESTPQTQHNTQQANLLREVKLKKTPKRRDGAHALDLSEKANLKDVIKTLKPVPRRREPPKVELTPRDQLLNDIRQSNVAYLKPVPLPKELESQETMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVM7</accession>
    <name>W5MVM7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVM7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012436"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012457.1">
        <property type="protein sequence ID" value="ENSLOCP00000012436.1"/>
        <property type="gene ID" value="ENSLOCG00000010156.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165158"/>
    <dbReference type="InParanoid" id="W5MVM7"/>
    <dbReference type="OMA" id="KGEKWID"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010156">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07222">
        <property type="entry name" value="Pat_PNPLA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033902">
        <property type="entry name" value="PNPLA4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF7">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="8"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="255" mass="28294" checksum="3E0F95E31D805AEB" modified="2014-04-16" version="1">AKMTLLNLSFAACGFLGIYHLGVACALQKHGHKLLRSVKAFAGASAGALVATVLVTGPDKIEHCKEFTYSFAEEVRRQRLGAMTPGYDFMQKLREGMEQILPPNAHEIAGNCLHVSITHSKTGKNCIVSSFASREELIKVLLASSFVPVYAGINAVEYKGEKWIDGGITNSVPILPEGRTILISPFSGQLDICPEHDGLLNFNIKVMNQNIMLSKDNFIRLNQALFPPEQKKMEKLCQNGYNDAVRFLRKENWFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVP5</accession>
    <name>W5MVP5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudouridine 5'-phosphatase</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVP5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012454"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012475.1">
        <property type="protein sequence ID" value="ENSLOCP00000012454.1"/>
        <property type="gene ID" value="ENSLOCG00000010173.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014753"/>
    <dbReference type="HOGENOM" id="CLU_045011_13_0_1"/>
    <dbReference type="InParanoid" id="W5MVP5"/>
    <dbReference type="OMA" id="IWCPHPG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010173">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="235" mass="26537" checksum="93CCCF54CC54107E" modified="2025-10-08" version="2">MSGSMEDTVMKFQPVTHVIFDMDGLLLDTERLYTVSFQEICDRFGKKYTWEVKSSVMGKKALEAAKIIRDSLELPMTAEELLEESQKKQEKLFPSAQLMPGAEKLIHHLHKHHIPMAVGTSSARVTFEMKTSRHKEFFHLFNHIVLGDDPQVKNGKPHPDAFLVCAERFNPPPSLEKCLVFEDAPNGVLAGLSAGMQVVMIPDENLNPDLTKEATLILKTMEDFKPELFGLPPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW99</accession>
    <name>W5MW99_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxiredoxin-6</fullName>
            <ecNumber evidence="12">1.11.1.27</ecNumber>
            <ecNumber evidence="13">2.3.1.23</ecNumber>
            <ecNumber evidence="2">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1-Cys peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Acidic calcium-independent phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutathione-dependent peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophosphatidylcholine acyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Non-selenium glutathione peroxidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35983"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12937"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="12"/>
    <dbReference type="EC" id="2.3.1.23" evidence="13"/>
    <dbReference type="EC" id="3.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01000139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW99"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012658"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012682.1">
        <property type="protein sequence ID" value="ENSLOCP00000012658.1"/>
        <property type="gene ID" value="ENSLOCG00000010332.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164279"/>
    <dbReference type="HOGENOM" id="CLU_042529_4_1_1"/>
    <dbReference type="InParanoid" id="W5MW99"/>
    <dbReference type="OMA" id="RLTMLYP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010332">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF4">
        <property type="entry name" value="PEROXIREDOXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 21" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="22">
        <location>
            <begin position="5"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="229" mass="25283" checksum="A2E5D5488B6EA79F" modified="2014-04-16" version="1">AMPGILLGDVFPNFEAETTIGKIKFHDFLGDSTTGWMTSFSHAVENRVTGVALDWLSLCAKLSEEFAKRNVKMIALSIDSVEDHLGWSKDIVAYNTEQPGSKLPFPIIADAKRELAVQLGMLDPDERDKDGMPLTARCVFVIGPDKKMKLSVLYPATTGRNFDEILRVIDSLQLTAQKRVATPVDWKPGECVMVLPNIPEAEAATLFPAGVFTKDLPSGKKYLRYTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWK4</accession>
    <name>W5MWK4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of KIF3A and KIF3B. Interacts with CIMAP3. Interacts with CLN3. Interacts with DCTN1. Interacts with FLCN. Interacts with AP3B1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWK4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012787.1">
        <property type="protein sequence ID" value="ENSLOCP00000012763.1"/>
        <property type="gene ID" value="ENSLOCG00000010399.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691804"/>
    <dbReference type="CTD" id="11127"/>
    <dbReference type="GeneTree" id="ENSGT00940000156386"/>
    <dbReference type="HOGENOM" id="CLU_001485_22_3_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010399">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000028">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="375"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="666"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="675"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="703" mass="80214" checksum="3276E38C9CD51871" modified="2014-04-16" version="1">MPSNKLDRSEKSEVSDNVKVVVRCRPINQKERMMGHKQAVTVDEIRGTITVNKIDSPNEPPKTFTFDTVFGPESKQLDVYNLTARPIVDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPELRGIIPNSFAHVFGHIAKAEGDTRFLVRVSYLEIYNEEVKDLLGKDQNQRLEVKERPDVGVYIKDLSGYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNQHVRMGKLHLVDLAGSERQGKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEDLKKKLEEGEEISGSEGSGSDEMEDEDGEVGEGGEKRRKRRGKKKVSPDKMVEMQAKIEEEKKALEAKLDMEEEERNKARAELEKREKDLLKAQQEHHLLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNNELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPLPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKSGRSRPKTGRRKRSSKPEAAIDSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWW8</accession>
    <name>W5MWW8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Calcium/calmodulin dependent protein kinase I</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630729.1">
        <property type="nucleotide sequence ID" value="XM_006630666.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202672.1">
        <property type="nucleotide sequence ID" value="XM_015347186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012877"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012903.1">
        <property type="protein sequence ID" value="ENSLOCP00000012877.1"/>
        <property type="gene ID" value="ENSLOCG00000010502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689592"/>
    <dbReference type="KEGG" id="loc:102689592"/>
    <dbReference type="CTD" id="794026"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156378"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="W5MWW8"/>
    <dbReference type="OMA" id="HERKICH"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010502">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000203">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="385" mass="43188" checksum="7101794735D88E76" modified="2014-04-16" version="1">MPLGDDGNAWKKKTSDIKEMYDFKEVLGTGAFSEVMLAEEKRTQRLVAIKCIPKKALEGKENSIENEIAVLHKIKHANIVTLEDIFESKSHLYLVMQLVSGGELFDRIVEKGFYTEKDASKLIRQILDAVKYLHDMGIVHRDLKPENLLYYSMEEDSKIMISDFGLSKIEGSGSVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIVHLMEKDPRKRYTCEQALQHPWIAGDTALDKNIHESVSAQIKKNFAKSKWKQAFNATAVVRHMRRLQLGTSQEGPSQNTPTSPCHGDLLVPEGEEDGGPLCDSSCSKTADGSEEHDGLLNCTYRCHPASRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX48</accession>
    <name>W5MX48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01014334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX48"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012957"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012984.1">
        <property type="protein sequence ID" value="ENSLOCP00000012957.1"/>
        <property type="gene ID" value="ENSLOCG00000010576.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155662"/>
    <dbReference type="HOGENOM" id="CLU_018371_0_1_1"/>
    <dbReference type="InParanoid" id="W5MX48"/>
    <dbReference type="OMA" id="EVFYANA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010576">
        <property type="expression patterns" value="Expressed in heart and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000003">
        <property type="entry name" value="Patatin-like phospholipase domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF46">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="16"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="51"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="389" mass="43557" checksum="C7D85DAECF27E45E" modified="2014-04-16" version="1">MSGNGSVQSGIPPFSISFSGSGFLAVYQLGAAQCLFDLAPGVIHAAPKVFGASAGSLAAAAVVCGANMECMRDEMLATARASRKHLLGPLHPSFNVFKLLECCLHRSLPDNAHELATGRLYISMTRLMDGENILMSDFMSKEELVQALLCSCFVPFYCGLKPPSFRGEHYIDGGFTNIQPIQDSSLTLTVSPFAGEVDICPCDQPPSFYHVHVNSFNFQFTLQNFFRMMDALFPPTSVALKRAYYHGYQDAEFFLQANGKQHRWEPFQLKMTEPGNIAHDSDCHMHSHLFGKDTSEMQIFIDLMGNRLPQRCTAACLHGCKEPDKMSIEIDHNSSVSARLISYLLLPYTLPAVMFFFFSHRLLIKLKDLPEEAFWLWEDMKQIAYFLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX66</accession>
    <name>W5MX66_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cystine/glutamate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Amino acid transport system xc-</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 7 member 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">xCT</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cystine(out) + L-glutamate(in) = L-cystine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:70995"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-L-cysteine(out) + L-glutamate(in) = N-acetyl-L-cysteine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:74567"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78236"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an L-alpha-amino acid(in) + L-kynurenine(out) = an L-alpha-amino acid(out) + L-kynurenine(in)</text>
            <dbReference type="Rhea" id="RHEA:71191"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc; this interaction mediates cell membrane localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200236.1">
        <property type="nucleotide sequence ID" value="XM_015344750.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX66"/>
    <dbReference type="FunCoup" id="W5MX66">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012975"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013002.1">
        <property type="protein sequence ID" value="ENSLOCP00000012975.1"/>
        <property type="gene ID" value="ENSLOCG00000010595.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698542"/>
    <dbReference type="CTD" id="23657"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166427"/>
    <dbReference type="HOGENOM" id="CLU_007946_3_0_1"/>
    <dbReference type="InParanoid" id="W5MX66"/>
    <dbReference type="OMA" id="PHWVWVL"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010595">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015811">
        <property type="term" value="P:L-cystine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000027">
        <property type="entry name" value="cystine/glutamate transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF323">
        <property type="entry name" value="CYSTINE_GLUTAMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="46"/>
            <end position="64"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="76"/>
            <end position="101"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="122"/>
            <end position="151"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
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            <begin position="163"/>
            <end position="180"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="192"/>
            <end position="214"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="234"/>
            <end position="254"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="266"/>
            <end position="288"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
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            <begin position="15"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004475"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00080978"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="498" mass="55074" checksum="789ED19F90A699A0" modified="2014-04-16" version="1">MSRTSAAVNNTYTRGYIRGNSKGSPGVESRNKDATGKVELRKKVTLLRGISIITGTIIGAGIFISPKGILKHSGSIGMSLVIWSACGVLSLFGALSYAELGTTIRKSGGHYTYMLEAFGPQIAFIGLWADLVAIRPSAIAVIALAFGQHILEPLFMPCAVPEIAVKLATAIGITAVMYLNSMSVSWSARVQICLFFCKLIAIAIIIIPGMIQLFKGETQNFENAFEAKEVQIMGLPLAFYSGMYAYSGWFYLNFVTEEVERPEKILPLAICISMAIVTFCYLLTNIAYYTVMTAEELLASEGVAVTFAEMLMGKFSVVVPVFVSLSCFGSMNGGIFAVSRTFYVASREGQLPEILSMIHIRRHTPLPAVIVLYPLALLTLFLGDLYSLLNFMSFVRWLFIGISVLGLIYLRHKHPEMQRTFKVPLFIPAFFSITCFFMVFLSLYSDPINTGIGFAITLTGIPAYYLFIVFNKRPKWFQRISDSITRFLQILLEVVPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXE4</accession>
    <name>W5MXE4_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Placenta associated 8, tandem duplicate 1</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
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    <dbReference type="EMBL" id="AHAT01013371">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006627083.3"/>
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        <property type="nucleotide sequence ID" value="XM_015337151.2"/>
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        <property type="nucleotide sequence ID" value="XM_015337157.1"/>
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    <dbReference type="AlphaFoldDB" id="W5MXE4"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013053.1"/>
        <property type="gene ID" value="ENSLOCG00000010660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692934"/>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="PLAC_motif_containing"/>
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        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
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    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="107" mass="12221" checksum="12820374B27369A9" modified="2014-04-16" version="1">MAVVTQPQFRGSAMPSEWKTGICDFCDDCGTCCYGFFCFPCLGCTIARDMDECCLCGLTMPMRSVYRTKYNIKGSLCTDFLYHQFCFVCSVCQLKRDIDKRKEEGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY18</accession>
    <name>W5MY18_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Paraspeckle component 1</fullName>
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    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PSPC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01003634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY18"/>
    <dbReference type="FunCoup" id="W5MY18">
        <property type="interactions" value="957"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013277"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013305.1">
        <property type="protein sequence ID" value="ENSLOCP00000013277.1"/>
        <property type="gene ID" value="ENSLOCG00000010832.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0115">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157358"/>
    <dbReference type="HOGENOM" id="CLU_027185_2_1_1"/>
    <dbReference type="InParanoid" id="W5MY18"/>
    <dbReference type="OMA" id="EQDMRMG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010832">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042382">
        <property type="term" value="C:paraspeckles"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12949">
        <property type="entry name" value="NOPS_PSPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12586">
        <property type="entry name" value="RRM1_PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000126">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012975">
        <property type="entry name" value="NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034522">
        <property type="entry name" value="PSP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08075">
        <property type="entry name" value="NOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="68"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="142"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="443"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="269"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="509" mass="57063" checksum="90B653DE2A13206A" modified="2014-04-16" version="1">KMANRNLKQVKIQNSSASPGARGPKRDAEPPAEQGAAAAELGAGDPLEMTLDLRNFRKPGEKTFTQRCRLFVGNLPTDLTEEDFKKLFAKYGDANEVFINRDRGFGFIRLETRTLAEIAKAELDGMILRNRPLRIRFATHGAALTVRNLSPVVSNELLEQAFAQFGPVERAIVVVDDRGRPTGKGFVEFAAKPAARKALDRCAEGAFLLTTSPRPAIVEPTEQFDDEDGLPEKLLQKTAQYHKEREQPPRFAQPGTFEFEYSSRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIEMRHEEERRRREEELMRQREQDELRRQQDGFKPNYMDNREQEMRMGDMGPRGAINMGDTYSPAAAGNQGPPQMMGMNMNNRGAISGTALGPGPAMGPEGAPNMGTPMIPDNGAMRSDRFPQGGPPPMGSPLVSRPGVEAQQAPMVGAGAVGGGPGGFGRGNQGGNFDGPNKRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYY9</accession>
    <name>W5MYY9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbonic anhydrase Xa</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not have a catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211407.1">
        <property type="nucleotide sequence ID" value="XM_015355921.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211408.1">
        <property type="nucleotide sequence ID" value="XM_015355922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYY9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013598"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013627.1">
        <property type="protein sequence ID" value="ENSLOCP00000013598.1"/>
        <property type="gene ID" value="ENSLOCG00000011070.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687497"/>
    <dbReference type="KEGG" id="loc:102687497"/>
    <dbReference type="CTD" id="641327"/>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155223"/>
    <dbReference type="HOGENOM" id="CLU_039326_7_1_1"/>
    <dbReference type="InParanoid" id="W5MYY9"/>
    <dbReference type="OMA" id="MYYQANT"/>
    <dbReference type="OrthoDB" id="5978072at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011070">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03121">
        <property type="entry name" value="alpha_CARP_X_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000002">
        <property type="entry name" value="Carbonic anhydrase-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041878">
        <property type="entry name" value="Alpha_CARP_X/XI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF208">
        <property type="entry name" value="CARBONIC ANHYDRASE XA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867015" evidence="3">
        <location>
            <begin position="22"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="4">
        <location>
            <begin position="31"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="37647" checksum="40F342FC2371760C" modified="2014-04-16" version="1">MEILWEIFFILQANFIVCISAQQNSPKIHEGWWAYKEVVQGSFVPVPSFWGLVNSAWNLCSVGKRQSPVNIETSHMIFDPFLTPLRLNTGGRKFGGTMYNTGRHVSLRLDKEHLVNISGGPMTYSHRLEEIRLHFGSEDGQGSEHLLNGQAFSGEVQLIHYNHELYTNYTEAAKSPNGLVIVSIFMKISESSNPFLNRMLNRDTITRITYKNDAYLLQGLNIEELYPETSSFITYDGSMTIPPCFETATWIVMNKPVYVTRMQMHSLRLLSQNQPSQIFLSMSDNVRPTQPLNNRCIRTNINFSVQGKDCPNNRAQKLQYRVNEWLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ20</accession>
    <name>W5MZ20_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L27, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ20"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013629"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013658.1">
        <property type="protein sequence ID" value="ENSLOCP00000013629.1"/>
        <property type="gene ID" value="ENSLOCG00000011095.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014660"/>
    <dbReference type="HOGENOM" id="CLU_143748_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ20"/>
    <dbReference type="OMA" id="NWDHTWV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011095">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000031">
        <property type="entry name" value="39S ribosomal protein L27, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868524" description="Large ribosomal subunit protein bL27m" evidence="9">
        <location>
            <begin position="19"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="147" mass="15968" checksum="1C34D21AC1E57FEC" modified="2014-04-16" version="1">MAALLSLLFKSRTGLLLSQQPLAVPARCASKKAGGSSKNVGGKSPGRRYGFKKMDAGNFVHAGNILATQRLIRWHPGSNVGIGRNNTLYALEDGVVRYTKEVYVPPPRSPEATKVICKLPKGSVLYKTFINIIPNKQEGAFKLVDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZF0</accession>
    <name>W5MZF0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 260</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5MZF0"/>
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        <property type="interactions" value="113"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013759.1"/>
        <property type="gene ID" value="ENSLOCG00000011193.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG7"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TMEM260-like"/>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
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        <location>
            <begin position="112"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="693" mass="76752" checksum="0E0121964F2AE411" modified="2014-04-16" version="1">MSAPRPGGPVSWGSWLLSGATAAGVAAVYLPRVQRTVPGGDSGELITAACELGVAHPPGYPVFTLLSRLALDLLPLGSPAYSVNVLAGLLGAMAAGALCFSVCRLHDAGPGAVLAGGAFALSRLAWQWAMVAEVFSLNNLFVGLLFSLAACFRSAETCGQRRKYGQLGALCCGLGLCNQHTFVLYVVVIVPWVLHRLHTHQELSVRTVVLLGTCFLVGFLPYLYLPLSSTLNRARWSWGDQTTLSGLVTHLLRAEYGTFSLAKTESRVGMARMLEAQLDHCVKDLSPPVLVLAGLGLILLSWDRGRRAITWLLAAMLCLYSLFFAWRANLDINRPLFLGVVERFWMQSDAVLCVLAGLGLSAACTGLGQRQGGGPLWRAAGWAVTAGLLAHLAQSNYRLCDQRDNTVVDRFARNVLRSIPSGSIVLTRGDLPGNSLRYLHFCHGLRPDLRLVEQEMMTYGWYVAKLGRHLPGVHFPGLWWDPVLSERGETFSLEQFLSHNSQRPVFACIGLPEGDPSWERAFSRWPWGVCDQLVPSSTPFHPEEWARRTRNLYNWTEPYKSFPRESWEKVANEEMWQARMKTAFFLFDLAERQSSAGGGKTHLYELSYSLYREIVETHRDYPSNWDKNLALASERLLRAGGGGHSPESLLNQSIAHFSLYLDKEGGDPQASAIREAIAHLERERRRLRELYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1P5</accession>
    <name>W5N1P5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox A11b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636125.1">
        <property type="nucleotide sequence ID" value="XM_006636062.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1P5"/>
    <dbReference type="FunCoup" id="W5N1P5">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014554"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014583.1">
        <property type="protein sequence ID" value="ENSLOCP00000014554.1"/>
        <property type="gene ID" value="ENSLOCG00000011836.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685753"/>
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    <dbReference type="eggNOG" id="KOG0487">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158311"/>
    <dbReference type="HOGENOM" id="CLU_079662_0_0_1"/>
    <dbReference type="InParanoid" id="W5N1P5"/>
    <dbReference type="OMA" id="HCYSADE"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011836">
        <property type="expression patterns" value="Expressed in intestine and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060272">
        <property type="term" value="P:embryonic skeletal joint morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="homeobox protein Hox-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="207"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="209"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="147"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="281" mass="32381" checksum="736CF6B27FB14542" modified="2014-04-16" version="1">MMDFDERVSVGSNMYLPSCTYYVSGADFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFRDYAIDASSKWHHRSNLSHCYSAEEIMHRDCLPAPTTMGEMFAKNSSTVYHSSSNSASNFYSGVGRNGVLPQAFDQFFETAYGNAEHQPSDYPGDKNTSKLPTTAMPGSDTYGETEAKERREESSSPESSSGNNEEKSNNTSGQRTRKKRCPYTKYQIRELEREFFFSVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLNRDRLQYYTTNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1U1</accession>
    <name>W5N1U1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1U1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014600"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014629.1">
        <property type="protein sequence ID" value="ENSLOCP00000014600.1"/>
        <property type="gene ID" value="ENSLOCG00000011873.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163984"/>
    <dbReference type="HOGENOM" id="CLU_1932329_0_0_1"/>
    <dbReference type="InParanoid" id="W5N1U1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011873">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039269">
        <property type="entry name" value="ANKFN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437:SF2">
        <property type="entry name" value="ANKYRIN REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437">
        <property type="entry name" value="WIDE AWAKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="131" mass="14355" checksum="1C194D6243A06BDD" modified="2014-04-16" version="1">CESPVSITGLTTVRHCDWDCESPVSITGLTTGGLYFVRVSAYNVKGYGPAQSSSPPSAAPSSWTQCCGVKQRSREQAAGVGKLLEQIQDPQYRGYFIETSRVHTPSKKLSVSRSLKHLFQSATKFVRSLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2L9</accession>
    <name>W5N2L9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box protein 38</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2L9"/>
    <dbReference type="FunCoup" id="W5N2L9">
        <property type="interactions" value="1558"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014878"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014907.1">
        <property type="protein sequence ID" value="ENSLOCP00000014878.1"/>
        <property type="gene ID" value="ENSLOCG00000012089.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSUC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013163"/>
    <dbReference type="HOGENOM" id="CLU_010774_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2L9"/>
    <dbReference type="OMA" id="ACITNNI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012089">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22107">
        <property type="entry name" value="F-box_FBXO38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042354">
        <property type="entry name" value="FBX38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14753">
        <property type="entry name" value="F-BOX ONLY PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14753:SF3">
        <property type="entry name" value="F-BOX ONLY PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="861"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1160" mass="130042" checksum="75509136512EBE39" modified="2014-04-16" version="1">MGPRRKSTRTLNSGGPAADPLRQQEPKDYINELSHEVLCHIFRYLPMQDIMCMECLSRKLREAVTLYLRVVKVVDLCAGRWWEYMPSGFTDSSFMMLLKKMPDLEQLYGLHPRYLERRRVRGHEAFSIPGVLEALQACPNLLGVETSHLELVEAIWNYMPQVHILGKFRNRNGAFPIPPENKLTIPVAAKIQTLHLVGVNVPEIPCISMLRHLYLKWVRLTKPQPFKDFLCVSLRTFVMRNCAGPTNSLKYVPLVTGLASARNLEQLELVRVPFLGGLIQHVVEDSWRSGGFRNLHTIVFGACKNALEVDLGYLIITAARRLHEVRIQPSLTKDGVFSALKMAELEFPQFETLHLGYVDEFLLQCKMSHSDLVKYGLADVIENPGIITDIGMKAVNEVFSSIKYLVIYNCPHLHNPHNWITDHSRWSRLVDLTLVRCHAIKLESFSQFVELLPSLEFISLDQMFREPPKGCARVGLSAGTGIGVSSALVSNQNSNNDNDNNNHQNNNNANAPPHRNENGEDAGAAERAAPEQRINAGGCFDSFAGGLAHLGCFDSSAGGLAHLGCFDSFSLLFSLTGPSGVQPAVKKPPIVVFDSDSEDEEEGDRMAAPTPLEPGYQEDGEKIMDTEEPVGHSSESSQYQINDQITLIIPSCRDARFPFASCFCYVIEIVTATRIEFHPQSTATNVRHKCKVTPLCSLEISSQESLRSSHLPTADSQNPQASGCFDTLGCLVCLSQDTGEFSFEWKELLIIISLYVCGLTAAPEEAVPATSSWLCGRVPPAPPSGPGEAGSEAPARTRCVLEPQADGQERTGGSSSSSEDGSHTGPRDSRAREGGHRRPVTRASSRQSCVPLVSESELSKPKPRPAVKRKRMADKSTSTSDPVTEDDHVQVLTLKSKNLVGITLTNCGITDLVLKECPKMMFIHATRCRVLKHLKVESAPIVNRFDYAQCKKLNMEQVLDQILRMPPERNRIIYMRPMQQVDSLTLERKIFSGPYPYHICIIHEFSNPPNVRNKVRVRSWMDTIANINQELIKYEFFPEATRTEEDVKRYPNYPWGRDIYTLEGVVDGAPYSMITDFPWLRSLRTAEPNSYARYDFEDDENTTIYAPRRKGQLSADICMETIGEEISELRQTRRGVFQRVVAIFIHYCDVRGEPVEDDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2U9</accession>
    <name>W5N2U9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3'-5' exoribonuclease 1</fullName>
            <ecNumber evidence="5">3.1.13.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Histone mRNA 3'-exonuclease 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01017257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627290.1">
        <property type="nucleotide sequence ID" value="XM_006627227.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2U9"/>
    <dbReference type="FunCoup" id="W5N2U9">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014958"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014987.1">
        <property type="protein sequence ID" value="ENSLOCP00000014958.1"/>
        <property type="gene ID" value="ENSLOCG00000012161.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687069"/>
    <dbReference type="KEGG" id="loc:102687069"/>
    <dbReference type="CTD" id="90459"/>
    <dbReference type="eggNOG" id="KOG0542">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="HOGENOM" id="CLU_037266_4_1_1"/>
    <dbReference type="InParanoid" id="W5N2U9"/>
    <dbReference type="OMA" id="MHSGQLM"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012161">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000015">
        <property type="entry name" value="3'-5' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000034">
        <property type="entry name" value="3'-5' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="SAP" evidence="20">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="39098" checksum="05E5EC824EFF6B4C" modified="2014-04-16" version="1">MADQKENVKFQDENKPQRNGSDSSAHSKPSCTQSGLTENDAHGPAASRGEFSNPVYKEIALANGHINRMNKDELRRKLSELKLETRGAKDVLKKRLKNYYKKQKLMQAVVTENSSTDTYYDYICVVDFEATCEENNPPDYLHEIIEFPMVLVNTHTLEIEDTFQEYVKPEINTNLSDFCVQLTGITQKTVDGADTFPTVLQKVVDWLQSKELGTKYKYAVLTDGAWDMSKFLNIQCRISRIRYPQFAKKWINIRKAYGNFYKLPRTRTKLSTMLEMLGMKYEGRPHCGLDDSRNIARIAMRMLQDGCPLRVNERMHAGQLMSVPSTARDEGAPAPRSPSCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2Z4</accession>
    <name>W5N2Z4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
            <ecNumber evidence="6">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EC" id="3.1.3.48" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01017259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2Z4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015003"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015032.1">
        <property type="protein sequence ID" value="ENSLOCP00000015003.1"/>
        <property type="gene ID" value="ENSLOCG00000012199.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159066"/>
    <dbReference type="InParanoid" id="W5N2Z4"/>
    <dbReference type="OMA" id="ELGEMDC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012199">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001714">
        <property type="term" value="P:endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903225">
        <property type="term" value="P:negative regulation of endodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14640">
        <property type="entry name" value="DSP_DUSP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000015">
        <property type="entry name" value="Dual specificity phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000093">
        <property type="entry name" value="Dual specificity protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF111">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="10">
        <location>
            <begin position="38"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="189"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="251"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="7">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="384" mass="42465" checksum="1BA0B36A59C122E9" modified="2014-04-16" version="1">IRGTYRGAIRKFTTIEMVTMDELCEMDCNVLKRLLKDDTGKCLVLDCRSFLAYSAGHIHGSVNIRCNTIVRRRAKGSVSLDQILAGDEEVKSRLKSGLYSAVILYDERTPHADSIKDDSTITLVLNALYRDSYGTEIYLLKGGYDKFFSEYPEFCLKTKTLSSNSSQSSLDATETNCTSCGTPHHDQGGPVEILPFLFLGSAYHASRKDMLDAMGISALLNVSSNCPNHFEGLYQYKCIPVEDNHKEDISSWFIEAIEYIDSVKDSNGRVLVHCQAGISRSATICLAYLMKKKRVKLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCSVEAASPSAGRGKSSTPTSPFVFSFPVSVAVHTQASSLTYLQSPITTSPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N323</accession>
    <name>W5N323_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein COX11, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with CNNM4/ACDP4. Interacts with RANBP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212139.1">
        <property type="nucleotide sequence ID" value="XM_015356653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N323"/>
    <dbReference type="FunCoup" id="W5N323">
        <property type="interactions" value="1102"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015032"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015061.1">
        <property type="protein sequence ID" value="ENSLOCP00000015032.1"/>
        <property type="gene ID" value="ENSLOCG00000012222.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698903"/>
    <dbReference type="KEGG" id="loc:102698903"/>
    <dbReference type="CTD" id="1353"/>
    <dbReference type="eggNOG" id="KOG2540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007512"/>
    <dbReference type="HOGENOM" id="CLU_045000_1_3_1"/>
    <dbReference type="InParanoid" id="W5N323"/>
    <dbReference type="OMA" id="NKLECFC"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012222">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="269" mass="30625" checksum="A77C8F2A32F741FE" modified="2014-04-16" version="1">MLVHLLFRKSFCWSENLAALTSKCRLYHKTFQNQVASRCQHGSPNHTPSQKTGLQHSGTQVRGMKAHRNPFTKDQQEEWRKRNKTILTYIVAAGVGMIGMSYAAVPLYRLYCQASGLGGTAVAGHDTEHVATMKPVRERVIKVTFNADVHASLQWNFRPQQTEIYLVPGETALAFYKAKNPTDKPIIGISTYNVVPFEAGQYFNKIQCFCFEEQRLNPNEEVDMPVFFYIDPEFDEDPRMARVKTITLSYTFFEAKEGQKLPLPGYSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7K7</accession>
    <name>W5N7K7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipase D2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01030316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7K7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016616"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016646.1">
        <property type="protein sequence ID" value="ENSLOCP00000016616.1"/>
        <property type="gene ID" value="ENSLOCG00000013467.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160229"/>
    <dbReference type="HOGENOM" id="CLU_000690_2_0_1"/>
    <dbReference type="InParanoid" id="W5N7K7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013467">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000048">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF121">
        <property type="entry name" value="PHOSPHOLIPASE D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="708"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="204"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="886" mass="101003" checksum="D04F38EA1B80170E" modified="2014-04-16" version="1">MASPEEDVVEATQATVLTRRLHRDDLPISEDELDCLREATDERPFLVIHHLQAVREKGVPLLLRDTPVTCKVDSTERHTTRSKTGHCYPLSFICNHITDPVKRKYRHFQELHRDLHSHKLMVNFLPLGRFAAERQQLGTMADELPTLHGADRARRASSKPKLLEDYLNVLLENSFCRNYHGMTGRCVVSHSHFLQELVACSETAVKEGFILKRSGGHRILGLNCFGHHQFCYRWSRRWLVVKDSFLLYMKPEAGVISFVLLFDPEFKVQVGRCYTDTKYGVCIQNFTRTLVIKCSSYRQAQWWSHEIHRLSEPCEFLQTHRFDSFAPPREDTLARWYINGREYFADVAQALEQAREEIFITDWWLSPEIYLKRPATSHYWRLDQILKRKAVRTSGVTLGSGSGWGQALGQTGLDAGTVLLLGWCGVRLGSGLDWGQVLGQAGVKLVLGSGLDWVSLSQSGDVVDSPGESNPVPETQGSVSDQRQVSPEDLTDNTRLWLGKDYSNFITKDWVQLDRPFEDNIDRTVVPRMPWRDMAAVVHGKAARDLSRHFIQRWNFTKIFKNKYKDDFYPCLLPKSHSAADSLPFIVPQTRSARVQILRSVDRWSAGTCECSIHRAYIDTIESSEHYVYIENQFFISCSDEKSVFNRIGDALVDRILRAHRSVYGHTHCSALSDVAVSASGAESSSAFLFLLFMNMVQLQHTVPCHPLAGSLMLIIVLILLLFICLMTTRCFQVIYSIGADDNCGSATTNTCEESSNTTGEVTLPCCRVLMSRVLLAADSDPCIDIQDPISDEFFQGVWNRIAESNADIYDMVFRSLPSNSIHNLRMLREYVSVERMAETDPDRAREELAGVRGYLVHFPLHFLCEEYLLPPLKSKERMVPMEVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7T0</accession>
    <name>W5N7T0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/potassium-transporting ATPase subunit beta-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7T0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016689"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016719.1">
        <property type="protein sequence ID" value="ENSLOCP00000016689.1"/>
        <property type="gene ID" value="ENSLOCG00000013536.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3927">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="HOGENOM" id="CLU_057702_1_1_1"/>
    <dbReference type="InParanoid" id="W5N7T0"/>
    <dbReference type="OMA" id="LARESWC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013536">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF26">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="250" mass="28073" checksum="E8A3C725B65453A0" modified="2014-04-16" version="1">STALILVFYLVFYTFLTGLFCLTMWVLLQTVDDYKPTYQDRLATPGLMIRPKCEALEIDYDIAKTESWDDYITALDSFLARESWCGERRRERGCTHLGSESESEGVLLWRVSRDELNGGKGGEEWVGGIIVNSVCLTHSLNVFQVIGMKPGSGESPYVTCGAKFGSATRTHTHLQFGDTHTLTAPVFPLQGLKAQRVNYSQPLVAVKFLNITFNSDVNVECKIVARDIKVDAEQRDKFAGRVSFKLRINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N858</accession>
    <name>W5N858_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Monocarboxylate transporter 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 16 member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3,3',5'-triiodo-L-thyronine(out) = 3,3',5'-triiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71815"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:71816"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:71817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3,3',5-triiodo-L-thyronine(out) = 3,3',5-triiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71811"/>
            <dbReference type="ChEBI" id="CHEBI:533015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:71812"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:71813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3,3'-diiodo-L-thyronine(out) = 3,3'-diiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71823"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:71824"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:71825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-thyroxine(out) = L-thyroxine(in)</text>
            <dbReference type="Rhea" id="RHEA:71819"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:71820"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:71821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Homodimer. Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632705.1">
        <property type="nucleotide sequence ID" value="XM_006632642.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206573.1">
        <property type="nucleotide sequence ID" value="XM_015351087.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206574.1">
        <property type="nucleotide sequence ID" value="XM_015351088.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206575.1">
        <property type="nucleotide sequence ID" value="XM_015351089.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N858"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016817"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016847.1">
        <property type="protein sequence ID" value="ENSLOCP00000016817.1"/>
        <property type="gene ID" value="ENSLOCG00000013636.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695786"/>
    <dbReference type="KEGG" id="loc:102695786"/>
    <dbReference type="CTD" id="6567"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159450"/>
    <dbReference type="HOGENOM" id="CLU_001265_59_1_1"/>
    <dbReference type="InParanoid" id="W5N858"/>
    <dbReference type="OMA" id="AWCNGSI"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013636">
        <property type="expression patterns" value="Expressed in liver and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015349">
        <property type="term" value="F:thyroid hormone transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070327">
        <property type="term" value="P:thyroid hormone transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17465">
        <property type="entry name" value="MFS_MCT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000156">
        <property type="entry name" value="monocarboxylate transporter 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000179">
        <property type="entry name" value="Solute carrier family 16 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF123">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="504"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="540" mass="58710" checksum="5D0F54157D6649AD" modified="2014-04-16" version="1">MRTESNNNAPVTETDGNPDSSENEKTGVRVVDTQPEATKCEVQIANPEHHAAGAKETHLKEKHDAAQLIETGCQEKTPASPSAGPDPEFVPPEGGFGWLVVLAATWCNGSIFGIQNSFGILHVMLVKDHQEADDQASQFKVAWVGALAMGMIFFCSPVVSMFTDHFGCRKTAVLGAVVAFIGLLTSSFATSLGLRYFTYGILFGCGASFAFQPSLVILGHYFRRRLGLVNGVVTAGSSVFSMGLPVLLKEVVEPLGLRATFQILSTFMLVQALLALTFRPLLPIVPDTTRSPLADTASCWHRSLAQIRKYFNLRVFRITTYRVWAFGVATAVLGYFVPYVHLISYVKEQFKETQKEWILLVCIGASSGVGRLVFGKIGDLIPGVKKIYMQVASFLVLGLMSMMIPQCRVFEGVIAVCLFLGLCDGCFITIMAPIAFELVGPMQASQAIGYLLGLMAVPMTAGPPIAGLLHDWFGNYHVAFYLAGVPPMVGGVVLFFVPYVHGQFQRPKPRDDPSKSKMLPPRSSANGELLPGFTDIETHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9M1</accession>
    <name>W5N9M1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Actinin, alpha 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9M1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017330"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017361.1">
        <property type="protein sequence ID" value="ENSLOCP00000017330.1"/>
        <property type="gene ID" value="ENSLOCG00000014057.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155548"/>
    <dbReference type="InParanoid" id="W5N9M1"/>
    <dbReference type="OMA" id="QQRWITV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014057">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097433">
        <property type="term" value="C:dense body"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090637">
        <property type="term" value="C:inner dense plaque of desmosome"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090636">
        <property type="term" value="C:outer dense plaque of desmosome"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030486">
        <property type="term" value="C:smooth muscle dense body"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990357">
        <property type="term" value="C:terminal web"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005915">
        <property type="term" value="C:zonula adherens"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051393">
        <property type="term" value="F:alpha-actinin binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017166">
        <property type="term" value="F:vinculin binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055001">
        <property type="term" value="P:muscle cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000113">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000105">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="31"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="144"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="746"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="809"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="430"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="915" mass="105749" checksum="831F1F6D51C63FB0" modified="2014-04-16" version="1">MDHYDGEENTDYRQQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPMTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKATIHEAWTEGKEDMLQQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQRICDQWDSLGSLTQKRSEALQRTEKLLETIDQLYLEFAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLSTAHDQFKATLPEADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTINPQEINTKWDKVRQLVPQRDQALIEEHTRQQNNERLRKQFANQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIINYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEIENQILTRDAKGISQDQMNEFRASFNHFDRDHSGTLGAEEFKACLISLGFDIANDAQKRTGIMDAEDFRTCLISMGYNLGEAEFARIMSIVDPNRLGVITFQAFIDFMSRETADTDTADQVMASFKVLAGDKNYILADELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFFETKYLGQKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA28</accession>
    <name>W5NA28_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA-binding protein 25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RNA-binding motif protein 25</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with LUC7L3 and SRRM1. Specifically associates with functional splicing complexes, including Sm proteins and U1, U2, U4, U5 and U6 snRNAs. Associates with exon junction complex (EJC) proteins, including APEX1, DDX39B, NCBP1, RBM8A and RNPS1. Interaction with NCBP1 is RNA-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NA28"/>
    <dbReference type="FunCoup" id="W5NA28">
        <property type="interactions" value="2131"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017487"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017518.1">
        <property type="protein sequence ID" value="ENSLOCP00000017487.1"/>
        <property type="gene ID" value="ENSLOCG00000014196.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111019"/>
    <dbReference type="HOGENOM" id="CLU_009855_0_0_1"/>
    <dbReference type="InParanoid" id="W5NA28"/>
    <dbReference type="OMA" id="DGCVNKK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014196">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12446">
        <property type="entry name" value="RRM_RBM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000004">
        <property type="entry name" value="RNA-binding motif protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000230">
        <property type="entry name" value="RNA-binding motif protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="88"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="PWI" evidence="17">
        <location>
            <begin position="710"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="218"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="426"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="261"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="482"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="579"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="803" mass="93937" checksum="1C13AA09AB693C5C" modified="2014-04-16" version="1">MSFPPHLNRPQIGIPPLPPGIPPPQFAGFPPAVPPGTPMIPVHMGIMTPTPAVLVPTTVPMVGKPMGPRKDFPGVRNKENEESGGPTTTVFVGNISEKASDMLIRQLLAKCGLVLSWKRVQGASGKLQAFGFCEYKEPESTLRALRLLHELQIGDKKLLVKVDAKTKAQLDEWKAKKRNSNGNAKPGDNADEEEELDEETKRRDQIVKGAIEGLIREYSSELNAPSQEADTHHRKKKKEKKEDSTLVAKNKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGKKEKERQEIERERREREKERERERERRDREREKEREREREKEREKERERERERDRDRERTKDKEREREREKERSRDRSKDRSRSREKSRDREKDKKRDREEDEEEAYERRKLERKLREKEAAYQERLKNWEIRERKKAREYAKEAEREEERRRETAKEAKRLKEFLEDYDDDRDDPKYYSNTRMWLTSRGSALQKRLRDREKEMEADERDRKREKEELEEIRQRLLAEGHPDPDAELQRMEQEAERRRQPPVKQEPESEEAEDEVEEGEEEEEEEEEDEPPESKPCLKPTLRPISSAPSVSSASGNASPNTPGDESPCGIIIPHENSPEAVPQEELRPRIGLSLKLGSSASPGQPNAGKRKKLAVDSVFNKFDDEESDELPRKRKLVPLDYGEDEKGADKGNVNSEEKRKNIKSLIEKIPTAKSELFSYPLDWSIVDTTLMERRIRPWINKKIIEYIGEEEATLVDFVCSKVMAHSTPQGILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKFGIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAW9</accession>
    <name>W5NAW9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01014852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAW9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017778"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017810.1">
        <property type="protein sequence ID" value="ENSLOCP00000017778.1"/>
        <property type="gene ID" value="ENSLOCG00000014444.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014125"/>
    <dbReference type="HOGENOM" id="CLU_018398_4_1_1"/>
    <dbReference type="InParanoid" id="W5NAW9"/>
    <dbReference type="OMA" id="DFDARYW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014444">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Release factor glutamine methyltransferase N-terminal" evidence="7">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase small" evidence="6">
        <location>
            <begin position="138"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="34169" checksum="8F3A23B4202B35F2" modified="2014-04-16" version="1">RPPPPSAGRPSVEECVDLWEGVFGERGVSEPRLSSEYIVAHVLGANTLASLARRGLAAVLTEAQTQRVWDLCARRLERMPVQYVIEEWDFRDLTLKMRPPVFIPRPETEELVGLVLTDLQHSRGPDQRGAVLASGPSLRFLEVGCGSGAISLSLLKSLPQARAVAVDRSPAAVSLAEENAHRLGLQDRLDLMELDVMTDAQAVLSHCGLFDFIVSNPPYVFSADMAALEPEILRFEDPGALDGGADGMDVIRHILSLAQQILPVGGKVYLEVEPRQPEMLQQRPGAGLQYTATHRDFSHRPRFCVLQRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC96</accession>
    <name>W5NC96_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="32">1.1.1.170</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>4alpha-carboxy-4beta-methyl-5alpha-cholest-8-en-3beta-ol + NAD(+) = 4alpha-methyl-5alpha-cholest-8-en-3-one + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47168"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:87047"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47169"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>4alpha-carboxy-4beta-methyl-5alpha-cholest-8-en-3beta-ol + NADP(+) = 4alpha-methyl-5alpha-cholest-8-en-3-one + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:46828"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87047"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:46829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>4alpha-carboxy-5alpha-cholest-8-ene-3beta-ol + NAD(+) = 5alpha-cholest-8-en-3-one + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47172"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:87055"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:47173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>4alpha-carboxy-5alpha-cholest-8-ene-3beta-ol + NADP(+) = 5alpha-cholest-8-en-3-one + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:46848"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87055"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4alpha-carboxyzymosterol + NAD(+) = zymosterone + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47164"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:143575"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>4alpha-carboxyzymosterol + NADP(+) = zymosterone + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33455"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:143575"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:33456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NAD(+) = 3-dehydro-4-methylzymosterol + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47160"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:47161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 3beta-hydroxysteroid-4alpha-carboxylate + NAD(+) = a 3-oxosteroid + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:34775"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136966"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 3beta-hydroxysteroid-4alpha-carboxylate + NADP(+) = a 3-oxosteroid + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:34771"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136966"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="31">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 34">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="32"/>
    <dbReference type="EMBL" id="AHAT01015665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC96"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018255"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018287.1">
        <property type="protein sequence ID" value="ENSLOCP00000018255.1"/>
        <property type="gene ID" value="ENSLOCG00000014825.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158229"/>
    <dbReference type="InParanoid" id="W5NC96"/>
    <dbReference type="OMA" id="STAHWFD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014825">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000252">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000251">
        <property type="entry name" value="Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0520">NAD</keyword>
    <keyword evidence="14 34" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 34" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="35">
        <location>
            <begin position="14"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00060653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00074569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="346" mass="38773" checksum="A1E6C4ECAD847DD3" modified="2014-04-16" version="1">MNSGVREVCKRCTVIGGSGFLGRHLVEKLLERGYIVSVFDILQGFELPGVTFHVGDLCDRQALLPALQGASVVFHCASPSPASDDRALFHRVNVEGTRTVLQACREAGVQRLVLTSSASVVFEGTDIKNASEDLPYARRPIDYYTETKIQQEKLVLEACDPGRGFLTVAIRPHGIFGPRDPQLVPILVDSARRGKMKFIIGFTLNMITLAYSPALLSWRAEGRVRPRELTPSCPRQAYHITNDEPVRFWEFLSRLLVGLGYEAPRYHLPYRLVYGLALLLWLLAVLLRPLLTFRPTFTPMRVALAGTHHYYSCARAKQELGYRPLVSLEEAITQTVRSYPHLRLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND12</accession>
    <name>W5ND12_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Acts as a plasma-membrane magnesium transporter. Can also mediate the transport of other divalent metal cations in an order of Ba(2+) &gt; Ni(2+) &gt; Co(2+) &gt; Fe(2+) &gt; Mn(2+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Co(2+)(in) = Co(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28578"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Mn(2+)(in) = Mn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28699"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ni(2+)(in) = Ni(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29831"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025726">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND12"/>
    <dbReference type="FunCoup" id="W5ND12">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000018553.1">
        <property type="protein sequence ID" value="ENSLOCP00000018521.1"/>
        <property type="gene ID" value="ENSLOCG00000015043.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183042"/>
    <dbReference type="HOGENOM" id="CLU_018207_3_0_1"/>
    <dbReference type="InParanoid" id="W5ND12"/>
    <dbReference type="OMA" id="NDKAYHG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015043">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF25">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="19">
        <location>
            <begin position="110"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="19">
        <location>
            <begin position="324"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="483" mass="52408" checksum="EABAD69C47BDCCC8" modified="2014-04-16" version="1">HAESRRHPERGRRASAHSEHGDRCYSETDAMLPEGRLSGEEEEEEEQEEEELREQEAARRMPKESPLAMALQILLPFLLAGFGTVSAGMVLDIVQHWEAFKNVTEIFILVPALLGLKGNLEMTLASRLSTAVNVGKMDSPIEKWNLIIGNLALKQVQATVVGFLAAVAAVVLGWIPEGKFQLSHAVLLCSSSVATAFIASLLQGIIMVGVIVGSKKTGINPDNVATPIAASFGDLITLAILAWISQGLYNCLETRPYVSSVVCAFFLALTPMWIVISSKHPASRTLLYSGWEPVITAMIISSIGGLILDKTVSDPNLAGIVVYTPVVNGIGGNLVAIQASRISTYLHFHSIPGEVPEEAKGCYCPCRTFCGSGANHRSAQVLLLLVLPGHLIFLYTIHLMKSGHTTLTPIFMTVYLVAALLQVFTLLCFADWMVHCMWRNGKDPDSFSIPYLTALGDLLGTALLALSFHFLWLIGDQDSDVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND37</accession>
    <name>W5ND37_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glypican-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND37"/>
    <dbReference type="FunCoup" id="W5ND37">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018546"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018578.1">
        <property type="protein sequence ID" value="ENSLOCP00000018546.1"/>
        <property type="gene ID" value="ENSLOCG00000015071.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244955"/>
    <dbReference type="HOGENOM" id="CLU_024658_4_1_1"/>
    <dbReference type="InParanoid" id="W5ND37"/>
    <dbReference type="OMA" id="MAPAREF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015071">
        <property type="expression patterns" value="Expressed in brain and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF4">
        <property type="entry name" value="GLYPICAN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11 14" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="12 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="45044" checksum="14F5C43C25E4B519" modified="2014-04-16" version="1">EAFDMVLRHARNYTALMFHGQFRGLAAAAAAPVAELFADISLYILGSDGSVDDMVNSFFDALFPPAYRRLLSPGAGGGASEECLRAAGRGAGAFGPYPKLIMTRMSRSLLATRVFLQALNLGIEVINTTDHLKFGKDCARSLLRLWYCPHCQGLLGARPCQGYCLAVMQACLSHVAEVQPHWEEYIEGLGALAASMRGVHDMENVLLKLYALVRDAVLHSYRNRLHLAALVSNMCGHSSQRLTRSAPPPHRPSGDPRGLKTLTFDPEETLAGRRREFIASLRGFSSFYSNLPEALCSRESAVLNDSLCWNGKEIAERFPSAGAKRAQAHGTETKNKAPEPVISQIIDKLKHINQLLRLVSAPRRRFKTRSGGGGAEQEEEVLESGDCDDEDECSGSGLGLPPRHRRLRIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDF6</accession>
    <name>W5NDF6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MCF.2 cell line derived transforming sequence</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017004">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018665"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018697.1">
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        <property type="gene ID" value="ENSLOCG00000015164.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_007130_1_0_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015164">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanine nucleotide exchange factor DBS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058918">
        <property type="entry name" value="KALRN/TRIO-like_spectrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
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    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056466">
        <property type="entry name" value="Spectrin_DBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF146">
        <property type="entry name" value="PROTO-ONCOGENE DBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23289">
        <property type="entry name" value="Spectrin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23323">
        <property type="entry name" value="Spectrin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="652"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="774"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="797" mass="89590" checksum="BBCE1594E433C0D1" modified="2014-04-16" version="1">SVVIETTIPMALKEVNEYLEKQIAYLSGGRGEESSVIITLPECSSFSEIPEEALAKVLMYLTLTPRKRQPGVKFIIILDRRLDTWMSIKTALARIATSQPLALDHLIQLCILTFIIAVSRICNVLAPLSSEQGYLVKLVVVMLSSVTDLLHYIDENQLTQDLGGTLEYCHSDWILLRSGIESFAVTVKEIAQMLQAFGTELAETELPKEVTATEFLLMSHTAKYRQLKEDIRTVMRKGRQLLSSLEASGTVEAASEQKRDASQDWETVQRLLAQLHDMETAFDGFFEKHHLKLQQYLQLCRFERSFREMELSLQQLLAQEREHPGMGDSMAQTEQFVRDLDTLDAEAQHEMSRAQILILQGHQLAASHHYALALICQRCNELRHLCDVLTAALRAKRESLGRAQDLLSRLEQAMCWCHEAAYLLADQQVDKFQSKEGAQAALRDIERFLEGTPPPLSGGPDALTLEFESILTPQLQAEIGRVHDKLGAMRGTIQNRQTSLRKLADKQVRPIQLVAPRPETPTRSKSPLFSPKHGEAAPRTVTHFCLALPSSALQVSTSRCPVTGVLSHRHPSLRKHQGTTCSTQGVKLGPRLSDLRDDSRAITSWCGQSLPRGIYSPWLYCSCAXQPGGARPTVAQAACALCCQGDLSDLGRVLMQGSFSVWISHRKGPTKMKDLARFKPMQRHLFLHERALLFCKRREEHGEGYDKTPSYSFKHCLKMSAVGITENVKGDIKKFEIWYSGREEVYVVQAPSVEVKLAWLSEIRKILTNQQKLTRDDSLQPSPASDHIQLSPPLFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDY8</accession>
    <name>W5NDY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDY8"/>
    <dbReference type="FunCoup" id="W5NDY8">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018847"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018879.1">
        <property type="protein sequence ID" value="ENSLOCP00000018847.1"/>
        <property type="gene ID" value="ENSLOCG00000015317.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160861"/>
    <dbReference type="HOGENOM" id="CLU_037860_0_1_1"/>
    <dbReference type="InParanoid" id="W5NDY8"/>
    <dbReference type="OMA" id="NESDEHH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015317">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="29"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="42587" checksum="9AEA5F01F0835F18" modified="2014-04-16" version="1">MQNKKIKMTSKAKVGKAGSKNKDDAPHELESQFILRLPPEYASTVRRVVQSGSVNLKDRLTIELHADGRHGIVRVDRVPLACKLVDLPCIIESLKTVDKKTFYKTADVCQMLVCTVDGDLYPPLEEPTGTTEPKNKKKDKDKDKKFIWNHGITHPLKNTRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSVRRWEVIAEDETKEADNHGSLANLDSSPGTSGHKLGHGSSVQRDELREIFNDISSSSEEEEDEGDRHEDEDLNIMDTEEDLERQLREKLNESDGPRDESDANSQIVMEYQVQINSVKAKLQETQEKKKHQEELIRKVENQALKNRFQTLLNEIIRQEERQLEQLASLQEQLDSLIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFJ2</accession>
    <name>W5NFJ2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase kinase</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01034930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFJ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019401"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019433.1">
        <property type="protein sequence ID" value="ENSLOCP00000019401.1"/>
        <property type="gene ID" value="ENSLOCG00000015753.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0576">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155483"/>
    <dbReference type="HOGENOM" id="CLU_006347_1_1_1"/>
    <dbReference type="InParanoid" id="W5NFJ2"/>
    <dbReference type="OMA" id="VIKLEPX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015753">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF17">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="16"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="539"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="392"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="22"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="877" mass="99020" checksum="2B0FEE1FCDFDE4AD" modified="2014-04-16" version="1">MNSSFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIVMMKDCKHSNIVAYFGSYLRRDKLWICMEYCGGGSLQDIYHVTGPLSESQIAYVSRETLQGLYYLHNKGKMHRDIKGANILLTDNGYVKLADFGVSAQITATLAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMSRALFLMTKSNFQPPKLKDKLKWTNNFHHFVKMSLTKNPKKRPTAEKLLQHPFVTQPLSRTLAIELLDKASNPDHSTYQDFDDDDPEPAVVVPHRIHSTNRNAREEKTRSEINFGQVKFDPPLRKETEPHHEPDLQLEYGQGSQGSYFFGGNKSLLKSVEEELHQRGHVAHLEDDDDDGENQHSTMKPKVPPPLPPKPKSIYIPQESPSGEDETTQGTIKRCPTSESPARPASYVPPRPPPPRLPPHKRSSLGNGVNASVNGEKENSFLQMGEKQSTMPPTVPIRKDKKDIPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCATSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLFPRRCTWLYVMNNSLLSISGKASQLYSHNLAGLFEYARQMQKLPVAIPTHKLPDKILPRKFAVSTKLPDTKGCQKCCVVRNPYTGHKYLCGAFQSSVILFEWVEPMQKFMLIKNIDFPLPCPLEVFEMLVVPEQQYPLVCVAVSKGTELNQVVRFETVNPNSTSSWFTESDAPQTCVIHVTQLERDTILVCLDRCIKIVNLQGRLKSSRKLSAELTFNFQIESIVCLQDSVLAFWKHGMQGRSFKSNEITQEISDNTRIFRLLGSDRVVVLESRPTDNPTAHSNLYILAGHENSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFN3</accession>
    <name>W5NFN3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFN3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019442"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019474.1">
        <property type="protein sequence ID" value="ENSLOCP00000019442.1"/>
        <property type="gene ID" value="ENSLOCG00000015794.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164736"/>
    <dbReference type="HOGENOM" id="CLU_697458_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFN3"/>
    <dbReference type="OMA" id="FWLPLIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015794">
        <property type="expression patterns" value="Expressed in testis"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007198">
        <property type="term" value="P:adenylate cyclase-inhibiting serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="27"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="210"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="396" mass="43795" checksum="7909C37A45446F74" modified="2014-04-16" version="1">SQSSLNFAFAIANCAVLLFTCFAGVFGNVLVIWAVCRQRSLQTSNNALLVNLATSDLLRCVIDCPLLLAIILWGYNINDLGSVLCHTQAVSFSLSCCVQLLTLASISAERYQAIAHPFKTSQRKRRVKIWIPLTWTLAVLVSSFCITIAEDTPVYVKCRGIRVEVLTSFDTFGLYILVPIWFASLTVIIGFYGRIFIVVRAHTKKIFDKGTSSLPPPAKKGKDVRIDGKQLADKTNSEEENKSKANNDTRWLGNRSVALPSISVIPEETVEERPTSHAVKTTPGCLSSLPSSTGEYSQGNLFNRERAKAGKEGKLAKRSGYIVITFIAFWMPLIISVLMNPFTLHNPGTVQKILEVETFAVALACMTAAVNPITYAVVNPQFRSEFRQLKTKYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGL0</accession>
    <name>W5NGL0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TAFA chemokine like family member 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGL0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019769"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019802.1">
        <property type="protein sequence ID" value="ENSLOCP00000019769.1"/>
        <property type="gene ID" value="ENSLOCG00000016048.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZT2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160682"/>
    <dbReference type="HOGENOM" id="CLU_126078_5_1_1"/>
    <dbReference type="InParanoid" id="W5NGL0"/>
    <dbReference type="OMA" id="TTSTHEM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016048">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040329">
        <property type="entry name" value="TAFA-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878:SF0">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="125" mass="13555" checksum="489D5EBF12E5D68F" modified="2014-04-16" version="1">MQLLKAVWALAGAAICCFVIFLIHSQFLKEGQLAAGTCEIVTLDRDSSQPRRTIARQTARCACKKGQIAGTTRARPACVDARIVKTKQWCEMLPCLEGEGCNLLVNKSGWTCTQPGGRVKTTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGS7</accession>
    <name>W5NGS7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Proton myo-inositol cotransporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">H(+)-myo-inositol symporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Solute carrier family 2 member 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">H(+)-myo-inositol cotransporter. Can also transport related stereoisomers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGS7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019836"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019869.1">
        <property type="protein sequence ID" value="ENSLOCP00000019836.1"/>
        <property type="gene ID" value="ENSLOCG00000016098.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684008"/>
    <dbReference type="CTD" id="101882338"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155870"/>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="W5NGS7"/>
    <dbReference type="OMA" id="ETGWRWM"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016098">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17360">
        <property type="entry name" value="MFS_HMIT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000105">
        <property type="entry name" value="proton myo-inositol cotransporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000177">
        <property type="entry name" value="proton myo-inositol cotransporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="18">
        <location>
            <begin position="76"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="640" mass="69496" checksum="3BB234529E5725C3" modified="2014-04-16" version="1">MSRKSSDNEYNLRSLSNLMGERRKKVNDDGERSLIKAPSSSSINSQGSTAAATGDLERAARKQFQQDVTPGFVYVLAIFSALGGFLFGYDTGVVSGAMLLLKKEMNLSALWQELLVSSTVGSAAVSALAGGALNGVFGRRVCILLASFIFSAGGIVLSVSPTKEILLVGRVVVGLGIGIASMTVPVYIAEAAPPQLRGQLVTINTLFITGGQFIASVIDGAFSYLPKDGWRYMLGLSVVPAVLQFLGFLFLPESPRWLIQKGKTQKARRVLSQIRGNQNIDEEYESIKSSIDEEEKEAGGGGLVIWRMLTYAPARRALIVGCGLQMFQQLSGINTVMYYSATILQMSGVKDDKMAIWLSAVTAFTNFIFTLFGVWLVEKVGRRKLTLGSLTGTSLSLLVLAIGFLLSAQHSPPVILHPTDPSTPNSTCSQYGFCDSCMLDPGCGFCYQENSSSIFASSCVPVNKASTEEAEWGRCSNATVMKEGIFWAYNYCPTPYSWTALLGLILYLMCFAPGMGPMPWTVNSEIYPLWARSTGNACSAGVNWTFNVLVSLTFLHVAQYLTYYGAFFLYSGLATVGFLFVFGCLPETKGRRLEEIESLFENQLCTCGASDSDEVSQDYGSVIHREENTGPENEIAYICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI90</accession>
    <name>W5NI90_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI90"/>
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        <property type="protein sequence ID" value="ENSLOCP00000020349.1"/>
        <property type="gene ID" value="ENSLOCG00000016472.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163895"/>
    <dbReference type="HOGENOM" id="CLU_000680_8_2_1"/>
    <dbReference type="InParanoid" id="W5NI90"/>
    <dbReference type="OMA" id="VAGHEMR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF85">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26844" checksum="A8AA72A4E1E8C5DE" modified="2014-04-16" version="1">EGELLRSEGTQRATGEELKTLQSKASVCDEFGSVPSGRLTADVAGHEMRVRSCKDHLHIGTWNIRSMNQGKLLWSRTKWNLYNSICWAIGHFKSDEHIIYYAGNERQRRNDVAFIIKSLARTVMKYNAVSKCLISICLRGTPINMTINQVYAPTTDAQEDKVVQFYEQVKAEIDRTCKQDIFIVMGDWNTKVGNKEEGQIVEKYGLGNRNKVGKRLIEFCKTNNLLLLANTFFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJU2</accession>
    <name>W5NJU2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJU2"/>
    <dbReference type="FunCoup" id="W5NJU2">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020901"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020937.1">
        <property type="protein sequence ID" value="ENSLOCP00000020901.1"/>
        <property type="gene ID" value="ENSLOCG00000016907.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234535"/>
    <dbReference type="HOGENOM" id="CLU_132985_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJU2"/>
    <dbReference type="OMA" id="CNLAPID"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016907">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF3">
        <property type="entry name" value="ALPHA-N-ACETYLNEURAMINIDE ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="179" mass="20663" checksum="5ECF1E4A1696F7DC" modified="2014-04-16" version="1">MMFLKCPRTKLSVWAALCVFVLCWLYIFPVYRLPSDKEIVNVVFKAGERYNYNQSCLAIEDFRKLLRDCCDPRNLFSVTKQNAPPGKILWYDGEFYYSHTVNNDSYSLFIEETPFQQPLKKCSVVGNGGILKHSGCGKEIDRADFIMRCNLPPLSEDYREDVGTKTHLVTANPSIIEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMM1</accession>
    <name>W5NMM1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exosomal polycystin 1 interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMM1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021918.1">
        <property type="protein sequence ID" value="ENSLOCP00000021880.1"/>
        <property type="gene ID" value="ENSLOCG00000017776.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694173"/>
    <dbReference type="KEGG" id="loc:102694173"/>
    <dbReference type="eggNOG" id="ENOG502S2I2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016499"/>
    <dbReference type="HOGENOM" id="CLU_079917_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMM1"/>
    <dbReference type="OMA" id="SRWNSEM"/>
    <dbReference type="OrthoDB" id="8434774at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017776">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140494">
        <property type="term" value="C:migrasome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160040">
        <property type="term" value="P:mitocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029250">
        <property type="entry name" value="ECPIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35658:SF1">
        <property type="entry name" value="CHROMOSOME 21 OPEN READING FRAME 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35658">
        <property type="entry name" value="RCG58666, ISOFORM CRA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15137">
        <property type="entry name" value="ECPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869985" evidence="1">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="23425" checksum="437B45F391A4B46F" modified="2014-04-16" version="1">MASVGLTFLLLISALCGRPAVAKHPNSTLLYDRNNSIRNCSCASEIQHCSHAQANILCSCRTIPRSSLNSVNVGLTYQGGLTVWVEDPWVLKDLVNHSCVQDLRLAACGSSPLPPEYLTLFDLKRLHIYNNAAGVRFPEQGLTITTLERSHEMSNIPPFQVSFLNVALLNGISLLKAYSVKNLPTIGEYFPNLPLPVSTEPAESQHCQVTFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN47</accession>
    <name>W5NN47_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN47"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022056"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022095.1">
        <property type="protein sequence ID" value="ENSLOCP00000022056.1"/>
        <property type="gene ID" value="ENSLOCG00000017953.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001195"/>
    <dbReference type="HOGENOM" id="CLU_068697_0_0_1"/>
    <dbReference type="InParanoid" id="W5NN47"/>
    <dbReference type="OMA" id="GMLLNRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017953">
        <property type="expression patterns" value="Expressed in larva and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039596">
        <property type="entry name" value="GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16840">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16840:SF8">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="6">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="6">
        <location>
            <begin position="94"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="232" mass="25887" checksum="BC25B4AAD71D0113" modified="2014-04-16" version="1">QFVCWQALLRCQQEKDCELAYNQYLAACEANIKGNRRQCPSHCINALIRLNQTRNGPDLETCECGQDAQCRRAKRAIEPCLPRRYPGDAAGIGCTEARQRCEDEPGCQSTLTAYLSNCGQLFNGRKCTVKCKSTIQQMLYIPSGVLLNRCVCDGVERPFCEVVKENMGKLCSVGDHGVSTSYGDTDDLYEDEDYELKVDREETDSESAGSFSRCSRSSPLLPLQLSLLLALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNX2</accession>
    <name>W5NNX2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Dopamine receptor D6b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG5"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DOPAMINER"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="82"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="121"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="215"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="283"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="452" mass="50475" checksum="015A931D206AD196" modified="2014-04-16" version="1">MENFTNATEVLSEGTDTDSTGLSVRALTGCLLFLLIVSTLLGNTLVCAAVIKFRHLRSKVTNFFVISLAVSDLFVAVLVMPWKAVTEVAGYWLFGSFCDTWIAFDIMCSTASILNLCIISVDRYWAIASPFRYERKMTQRVAFIMIGVAWTLSILISFIPVQLNWHKATVEDAPANFSNHTENCDASLNRTYAISSSLISFYIPVAIMIGTYTRIYRIAQTQIRRISSLERAVEHAQNCHHHPNDCPHEHSLKTSFKKETKVLKTLSIIMGVFVFCWLPFFVLNCMVPFCDINTYELGELPCVSDTTFNIFVWFGWANSSLNPVIYAFNADFRKAFSTILGCNRFCSSTTVEAVNFSNELVSYHHDTTFQKDAPIVFTAGQLPHAVAQSGEEVSIPFDKVSVLSDTSRSHKNLLLPAAVQFECEAEISLDTITPFTSAGPNECDAIPGQIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNX8</accession>
    <name>W5NNX8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 3-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15461"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:9831"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:70975"/>
            <dbReference type="EC" id="2.8.2.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01004086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202196.1">
        <property type="nucleotide sequence ID" value="XM_015346710.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNX8"/>
    <dbReference type="FunCoup" id="W5NNX8">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022337"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022378.1">
        <property type="protein sequence ID" value="ENSLOCP00000022337.1"/>
        <property type="gene ID" value="ENSLOCG00000018236.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691080"/>
    <dbReference type="KEGG" id="loc:102691080"/>
    <dbReference type="CTD" id="557111"/>
    <dbReference type="eggNOG" id="KOG3704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164759"/>
    <dbReference type="HOGENOM" id="CLU_017703_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNX8"/>
    <dbReference type="OMA" id="SHWRMER"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018236">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000674">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF18">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868094" description="Sulfotransferase" evidence="14">
        <location>
            <begin position="18"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="15">
        <location>
            <begin position="63"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="10">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="277"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="36848" checksum="41827CD0E3ACD4A9" modified="2014-04-16" version="1">MAHLLFSAFFLAAQTHAVPKEYFQQTEGEVLDSLKLQVGLLANETENYPPQVKPPGTNRRIPQSIIIGVRKGGTRALLEMLDIHPNIVVAATEVHFFDWDENYVKGFDWYKNLMPFSYEHQITVEKTPGYFTSPLAAERIHEMNSSIKLLLILRDPTERVISDYTQVYYNRLENRKPVQVIEDIVIRNGALNTKYKAIQRSLYDLHMENWLKYFSLDQIHIVDGDTLIKDPLPELQKVEKFLSLPPRIMPSNFYFNQTKGFYCIRSDGRERCLHESKGRPHPVVNNTVLEQLRAYFREHNNNFYRMVNRSFNWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP48</accession>
    <name>W5NP48_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member A4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP48"/>
    <dbReference type="FunCoup" id="W5NP48">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022407"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022448.1">
        <property type="protein sequence ID" value="ENSLOCP00000022407.1"/>
        <property type="gene ID" value="ENSLOCG00000018306.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182827"/>
    <dbReference type="HOGENOM" id="CLU_024645_5_1_1"/>
    <dbReference type="InParanoid" id="W5NP48"/>
    <dbReference type="OMA" id="SSCVVMI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018306">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="322" mass="36007" checksum="6FE78F473B3386DB" modified="2014-04-16" version="1">MIVIKKVRSLDVSTQQKSRFTQTGWLFLLVFSVFIYGSHAPLITLCKVDGKVPFSPSSCVVLIEVTKLLISLGLLLIQDLRSLQVTLSWGHMVPYAFPAVLYAINNNLVVYMQLYMDPSTFQVLSNLKIISTALLYSLFLNKRLLPVQWWAVCLLMMAGVCHTYSSLDHTEEEKGEAVVGTQFHISVLGLVLVLSYCFVSGLAAVYTELILKTQRLPLNLQNLFLYVFGVAVNCCAHFTNAGPGFFEGYSWVVWLIVTTQALNGLFMSLVMKHSSNITRLFVISCSMLVNAVLSVSLFSLQLTPLFFVSVVLIGLAIYLYYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV81</accession>
    <name>W5LV81_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNase P subunit 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_006643248.1">
        <property type="nucleotide sequence ID" value="XM_006643185.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LV81"/>
    <dbReference type="FunCoup" id="W5LV81">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000038"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000038.1">
        <property type="protein sequence ID" value="ENSLOCP00000000038.1"/>
        <property type="gene ID" value="ENSLOCG00000000031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689504"/>
    <dbReference type="KEGG" id="loc:102689504"/>
    <dbReference type="CTD" id="10556"/>
    <dbReference type="eggNOG" id="KOG2363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000883"/>
    <dbReference type="HOGENOM" id="CLU_048451_2_1_1"/>
    <dbReference type="InParanoid" id="W5LV81"/>
    <dbReference type="OMA" id="CYGPGIT"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000031">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000031">
        <property type="entry name" value="ribonuclease P protein subunit p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="29572" checksum="95DA8B0197E85635" modified="2014-04-16" version="1">MAVFADLNINYTDDKKQLRDIIETAAHLGYSTVAINHLIDLQPKKQEIRKPASVDELFRQLPVVQGKSRPIKVLNRLTLGVSDASHFRPSADLKYYDLVAVVPKTEKLFHAACMTFDVDIICITVTEKQPFFFKRPPVNGAIDRGVFFEITYTPAIKDSTMRRYTISNALSLMQTCKGKNVIISSGAEKPLDLRGPYDISNLGLVFGLSEGDAKAAVSTNCRATLLHAETRKTALGVIHTIKKTQTVEEDQEQEAPASKRAKTKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVV2</accession>
    <name>W5LVV2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVV2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000259"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000259.1">
        <property type="protein sequence ID" value="ENSLOCP00000000259.1"/>
        <property type="gene ID" value="ENSLOCG00000000227.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_723532_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVV2"/>
    <dbReference type="OMA" id="QGDESAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057810">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23057">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="318" mass="35542" checksum="88F29AC8F6CB55AD" modified="2014-04-16" version="1">PPPGTGRKAGRQATRREGERAEPGCPAAACVPGSRGVGLCRCATAQTPARPPKAGVADFSCHFVTTMERGSASREGRVRHAVRFVLRAGGERTELASKDEFEEEVISNALHLEPEHVYCIQGFLGKKCFDVSIILENLVRGVWEVANRRKDEKPFVDYYVEPLFARELRTLTVAMFNSYVPEEDIVCFLKRFVDIRGIGVKVIDKRGFWTGKRRFKVRFKGSAPDGLLHPPATFEIGPNQGYLYYYGQPLVCRRCRKEGHVVDCRELVCRRCEGVGHFTSEYKEEMKCNLCGGEGHVFKDCPLHVVHGSAEARPGADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWY1</accession>
    <name>W5LWY1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWY1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000638"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000640.1">
        <property type="protein sequence ID" value="ENSLOCP00000000638.1"/>
        <property type="gene ID" value="ENSLOCG00000000577.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154838"/>
    <dbReference type="HOGENOM" id="CLU_1075806_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWY1"/>
    <dbReference type="OMA" id="YGVQWGR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000577">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF87">
        <property type="entry name" value="COLLAGEN ALPHA-1(VI) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865238" description="VWFA domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="144"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="229" mass="25402" checksum="0C323F2CD7537C10" modified="2014-04-16" version="1">LSLSLSLSLLLALCDSSAFNLETENVKVFEDQSQWFGYRVLQLSPGRDKWVIVSAPLERNGTLFKCDYKSGTCSRVIDSGPGTMGLSLTARQPDELVVCGPRLQHSCGKNEYLNGVCFTLDRSLNVLPSNYTPGYQECTSKPVDIVFLIDGSGSIKRNEFSQMKVFMQEIINRFQGRGANFAVVQYSNYIRLEFDFNKYTSARSAADLINNIVQIGSSTRTAAGIKYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX34</accession>
    <name>W5LX34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LX34"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000694.1">
        <property type="protein sequence ID" value="ENSLOCP00000000691.1"/>
        <property type="gene ID" value="ENSLOCG00000000625.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LX34"/>
    <dbReference type="OMA" id="NGYHIAW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000625">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866950" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="127" mass="14009" checksum="99653AD294BF73FE" modified="2014-04-16" version="1">LSYSIQSITFSISCSVLFLSECSGQVTVTQSPSVRSVLQGQTLTVSCKTSSPVYSDNNGYHIAWYHQKSEGAPRLLIYLVNKLNSVTQSWFSGSGSGTDFTLTIKGVRTEDAADYYCQSLHNINSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX73</accession>
    <name>W5LX73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LX73"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000730"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000733.1">
        <property type="protein sequence ID" value="ENSLOCP00000000730.1"/>
        <property type="gene ID" value="ENSLOCG00000000636.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153770"/>
    <dbReference type="InParanoid" id="W5LX73"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000636">
        <property type="expression patterns" value="Expressed in bone element and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003350">
        <property type="entry name" value="Immunoglobulin light 3 variable 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="1">
        <location>
            <begin position="29"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="115" mass="12768" checksum="683E8E78B8A36D9E" modified="2014-04-16" version="1">ICRLFCVQSIVATTAAVGQALMSTAFIRRFSPLSSYLSWYLQKLGQAPQLLVYHGTTHQSGVPDRFAGIGSGTAFPLTFTVVQAEDAGDYYCQQRYSYPFTVIHAMKQKITNTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXP0</accession>
    <name>W5LXP0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lysosome-associated membrane glycoprotein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Lysosomal membrane glycoprotein which plays a role in the unfolded protein response (UPR) that contributes to protein degradation and cell survival during proteasomal dysfunction. Plays a role in the process of fusion of the lysosome with the autophagosome, thereby modulating the autophagic process. Promotes hepatocellular lipogenesis through activation of the PI3K/Akt pathway. May also play a role in dendritic cell function and in adaptive immunity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Monomer. Interacts with FURIN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Lysosome membrane</location>
            <topology evidence="3 19">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the LAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXP0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000901.1">
        <property type="protein sequence ID" value="ENSLOCP00000000897.1"/>
        <property type="gene ID" value="ENSLOCG00000000806.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695092"/>
    <dbReference type="KEGG" id="loc:102695092"/>
    <dbReference type="CTD" id="27074"/>
    <dbReference type="eggNOG" id="KOG4818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182899"/>
    <dbReference type="HOGENOM" id="CLU_055379_0_1_1"/>
    <dbReference type="InParanoid" id="W5LXP0"/>
    <dbReference type="OMA" id="ISANSLW"/>
    <dbReference type="OrthoDB" id="9428839at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000806">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000006">
        <property type="entry name" value="Lysosome-associated membrane glycoprotein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048528">
        <property type="entry name" value="Lamp2-like_luminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF30">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01299">
        <property type="entry name" value="Lamp2-like_luminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00336">
        <property type="entry name" value="LYSASSOCTDMP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51407">
        <property type="entry name" value="LAMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="14 19" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 19 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866969" description="Lysosome-associated membrane glycoprotein 3" evidence="22">
        <location>
            <begin position="21"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="23">
        <location>
            <begin position="98"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="217"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="296" mass="31821" checksum="300B12DB777781B8" modified="2014-04-16" version="1">MEPLLHVFIIVAVCAAHACGFTQQGDVRSDTGPLPAPRPAAGSRPPSEAAASGSHTATRSAEQETPPAAGHPPVSSQSPVPPLSSAGATLSPRPAAPLTGDYTVTDSKHQVCIKARLGAQFMVKKNKKTAYFNISPTSTQAQGQCGQSTAWLLLSFSEGFVNFTFVKEGSISYVDEIRASLHPTATCVSCTIPGEEYTGKIVNVKLFKTADGHAYTCNSQYEVFLAENLSLRIVNTQMQAFNITNGRFGQEEECSADWKRRTIPIILVATVVGLSLIVILSCLIIREHDRGRYERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXT5</accession>
    <name>W5LXT5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Microtubule associated protein 1S</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXT5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000942"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000946.1">
        <property type="protein sequence ID" value="ENSLOCP00000000942.1"/>
        <property type="gene ID" value="ENSLOCG00000000850.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160221"/>
    <dbReference type="InParanoid" id="W5LXT5"/>
    <dbReference type="OMA" id="HESPELH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000850">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="InterPro" id="IPR026074">
        <property type="entry name" value="MAP1"/>
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        <property type="entry name" value="MAP1A/B/S-like_MBL"/>
    </dbReference>
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        <property type="entry name" value="MAP1B/S_N"/>
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        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843:SF11">
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    </dbReference>
    <dbReference type="Pfam" id="PF25281">
        <property type="entry name" value="MBL_MAP1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Microtubule-associated protein 1A/B/S-like MBL-like" evidence="3">
        <location>
            <begin position="181"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="638" mass="70511" checksum="2597E0B9F06420BB" modified="2014-04-16" version="1">SGIRSWDVDLTACNLDEQLKLFVSRHSATFSEEVKGQRTLHHSGDVLETHVVVNPSDDFVCSEVRALISDVSRHKLLVLAGQCVEEEGDLVLQMGSFSLQDFIQIFADKEIGELLSSADPTHKASLTLAGPNSGEWKNSVLEKHNLQDFIEIKTNPPTILPEMEGLQEFTEYLSESLEPRSPFELLEPPGTVGFLKLSRPCCYIFPGGRGDSAFFAVNGFNVLVNGGSDSRACFWKLVRHLDRIDSVLLTHIGVDNLPGINSLLQRKAAELEDDQSAGSQSNEDWLKNLISPELGVVFLNAPERLKTLPSDPKVLRSCDQVALTLQYLERLSVQPEPLCRTNGAVIEPVILFQKMGVGRLELYVLNPVKGSKDLETLMQNWPGNGSPKGSEISLQCLVSICALLVWHPANPLEKIIRVLFPGCTPQGKILEGLEKLKHLDFLKHPVVSVKDLESSKSDKQPKRAESRESLKSVPKDSRPSSGTLKEKLGRGDKKPDTKAKSKVSGDALKEGPCPKDVDEKSKAKEEEKQKVDVKPKTSKEKPATKKEPAKDEKKEVKKKEEKLPAKKEENGEKKKEMVKRDTAAAKPKKDVKPEPKKEVKKEVKAEERKLTKQPVKEVKRAPGAAASAVAESKKPAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY43</accession>
    <name>W5LY43_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Dapper homolog 2-like</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5LY43"/>
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        <property type="gene ID" value="ENSLOCG00000000945.1"/>
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    <dbReference type="HOGENOM" id="CLU_888412_0_0_1"/>
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        <property type="component" value="Linkage group LG28"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="313" mass="32380" checksum="878D00C58660DE06" modified="2014-04-16" version="1">MITLTAWLMGSRASARAALRDREGLWAARLSAVPLCRRPAVRTARTVAGPPSPGTPWRCPTQKRCPPTRLAPRGPPATPGPAQGSTRSVAAPCRPPAAPSAARGPGGPGLVARTPPTTAPPSGRGPDCSPPLSSRRGPRGGSGGRCPQVTWKYSAASCRSPTFAPGWGAGPQGRPPLPRTALQRPSAGTPDSRWTPSSARTWCRGRPRRCTPTPAPSTRWRCRAPCTRPAAPRTPSRMRVAGGRRCPGVPAAPALRTLLARPGQIATSASCSGGTGAGPARCCSPPWTPAPRAAGKRRACRCRPSAAAVPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYE3</accession>
    <name>W5LYE3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYE3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001150"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001154.1">
        <property type="protein sequence ID" value="ENSLOCP00000001150.1"/>
        <property type="gene ID" value="ENSLOCG00000001027.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502T0DU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3177734_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYE3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001027">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="47" mass="5334" checksum="00654D69C6A24836" modified="2014-04-16" version="1">PSSPMKTAYKMDRSTLSCEKFPDSFSGARRKSAMALRWEAGMFTNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYG4</accession>
    <name>W5LYG4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Forkhead box F1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01010681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641203.1">
        <property type="nucleotide sequence ID" value="XM_006641140.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYG4"/>
    <dbReference type="FunCoup" id="W5LYG4">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001171"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001175.1">
        <property type="protein sequence ID" value="ENSLOCP00000001171.1"/>
        <property type="gene ID" value="ENSLOCG00000001042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685631"/>
    <dbReference type="KEGG" id="loc:102685631"/>
    <dbReference type="CTD" id="2294"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161035"/>
    <dbReference type="HOGENOM" id="CLU_039845_1_0_1"/>
    <dbReference type="InParanoid" id="W5LYG4"/>
    <dbReference type="OMA" id="KPHGQTA"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001042">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20049">
        <property type="entry name" value="FH_FOXF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000071">
        <property type="entry name" value="Forkhead box F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051770">
        <property type="entry name" value="Forkhead_box_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46262">
        <property type="entry name" value="FORKHEAD BOX PROTEIN BINIOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46262:SF1">
        <property type="entry name" value="FORKHEAD BOX PROTEIN F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="53"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="53"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="381" mass="41292" checksum="02C882B848DADC63" modified="2014-04-16" version="1">MTAEVQQPLQTPAQSSPMSAAPEKPNGQTSVMETASSTTKTKKTNAGIRRPEKPPYSYIALIVMAIQSSPTKRLTLSEIYQFLQSRFPFFRGSYQGWKNSVRHNLSLNECFIKLPKGLGRPGKGHYWTIDPASEFMFEEGSFRRRPRGFRRKCQALKPMYSMMNSLGFNHIPESYNFQGAGGALGCPPNSLSLDSGIGMMNGHLASNVEGMGLPGHSVSHLSANTGHSYMGSCTGSSGADYPHHDNSVPASPLLTSGGVMEPHSVYSSAASAWAPAASASLNNGASYIKQQPLSPCNPGANPLQPSLPTHSLDQSYLHQNGHSTTELQGIPRYHSQSPSVCDRKEFVFSFNAMTSSAMHSTGGGSYYHQQMAYQDIKPCVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ84</accession>
    <name>W5LZ84_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LZ84"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001441"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001446.1">
        <property type="protein sequence ID" value="ENSLOCP00000001441.1"/>
        <property type="gene ID" value="ENSLOCG00000001269.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162286"/>
    <dbReference type="HOGENOM" id="CLU_000680_32_6_1"/>
    <dbReference type="InParanoid" id="W5LZ84"/>
    <dbReference type="OMA" id="WECTSIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001269">
        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF26">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="258" mass="29549" checksum="94AF596F51694720" modified="2014-04-16" version="1">TRRLLDDGEIADVFPVTNGVKQECVLAPALFSMMFSAMQLDAFRNSEEGTHIRYRTVKEMVIGDFLFVDDCTLNAASEQDMQASMDRSYAACNNFGLTISTQKDRSLPPGKPHQKPCITAKGLTLNALDSFTYLGRNLSRHVNIDEEINCRIAKASSRLQANFWECTSISLNTKLKVYQAIVLPMLLYACKRWTIYSRHVRQLNHVHMTCLRKVLKIRWHDKIPDTRVWTGTGQHNIHTLLQKTRARWAGHVLHMDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZB5</accession>
    <name>W5LZB5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZB5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001472"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001477.1">
        <property type="protein sequence ID" value="ENSLOCP00000001472.1"/>
        <property type="gene ID" value="ENSLOCG00000001299.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SQQV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_5_1_1"/>
    <dbReference type="InParanoid" id="W5LZB5"/>
    <dbReference type="OMA" id="HSYATFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001299">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001878">
        <property type="entry name" value="Immunoglobulin heavy variable 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865364" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="123" mass="13471" checksum="E722AC860A18A970" modified="2014-04-16" version="1">MNMSLLFFTSTAFLLLSCVSSDIVLTQSGPQTKKPGKSATLSCTFSGFTLSGSGYYAGWVRQDPGKPLEWLGMIRGDKAYTYYADSVKGRFTVSVDSSSKSYLEMSSLKTEDTAVYYCARDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZC0</accession>
    <name>W5LZC0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01025129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZC0"/>
    <dbReference type="FunCoup" id="W5LZC0">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001477"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001482.1">
        <property type="protein sequence ID" value="ENSLOCP00000001477.1"/>
        <property type="gene ID" value="ENSLOCG00000001279.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682732"/>
    <dbReference type="KEGG" id="loc:102682732"/>
    <dbReference type="CTD" id="150094"/>
    <dbReference type="eggNOG" id="KOG0586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154989"/>
    <dbReference type="HOGENOM" id="CLU_000288_87_2_1"/>
    <dbReference type="InParanoid" id="W5LZC0"/>
    <dbReference type="OMA" id="IAQIWQH"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001279">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14071">
        <property type="entry name" value="STKc_SIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14408">
        <property type="entry name" value="UBA_SIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000154">
        <property type="entry name" value="Serine/threonine-protein kinase SIK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034672">
        <property type="entry name" value="SIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF47">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="26"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="301"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="642"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="758" mass="83795" checksum="618B947600C5DFD1" modified="2014-04-16" version="1">MVIMSECSPEPVPTQPHGRPLQVGFYEIIKTLGKGNFAVVKLARHKVTKTQVAIKIIDKTRLNSSNLEKIYREVQIMKLLNHPHIIKLYQVMETKDMLYIVTEYAKNGEMFDYLSSNGRLSEDEARKKFWQILMAVDYCHRHHIVHRDLKTENLLLDANMNIKLADFGFGNFYNAGDPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGASLPALRQRVTEGRFRIPYFMSQDCENLIRRMLVVDPAKRISIAQIKQSRWMLADPTAQQQALSVSITDYNSNLGDYSEPVLGIMQTLGIDRQRTIESLQNSSYNHFSAIYYLLLEKVREHRSSQLSRQCSTRPPRHRSGPDFAPAEAPQEPSDCLRTACPFPAVQSAPYHSDADCDSAGLFQRVVFPLEASLNGLLQNRSISPNSLLENTISEEVRPSDLEEEEGLQVAAPLCPPNNTSRRHTLAEVSAHFHQCSPPCIVVSSVNPSEGASSDSCLKSSISTGPALVKGGYTNFLASAGGTGAYIQTGTPFTQSSTLLLQPQGYQATASLPGANFQEGRRASDTSLTQGLKAFRQQLRKNTRSKGLLGLNKIKGPARQACPCPCPRAGRGALGPSLGTSSDRRSLLEEVLHQQRMLQLQHQQQQQQPSLFLSQQPAPLPSGLFPPAALLSESNALDATPLLLRGCHTPGPQRQLLSQPPERLSPISSAAHLLEARLHIHGGPTYLPRFSFQLPDCEMEDLTSSQMSSCVLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0L9</accession>
    <name>W5M0L9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:dkey-205h13.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01034568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0L9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001926"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001931.1">
        <property type="protein sequence ID" value="ENSLOCP00000001926.1"/>
        <property type="gene ID" value="ENSLOCG00000001674.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R7BT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008152"/>
    <dbReference type="HOGENOM" id="CLU_922998_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0L9"/>
    <dbReference type="OMA" id="GLCADYK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001674">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039675">
        <property type="entry name" value="CILP1/CILP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031:SF4">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="113"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="205"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="33640" checksum="CF4367F3E87A3B84" modified="2014-04-16" version="1">TFGPYYTCTEEWSLACRTGWFDDGDVTILGDKELLADLQRNHGDHICENPVDMEAQTISGRTSVLPVLMFSSYDATYGFACVNADQSDSRCEDYRVRFTCPEVFCSVSQECRTGWFSSDDPSGVGDIESITHLLQKYPEHICYNPIAIHAQTVAGIPAKHTGNTFLTYDVTYGFACINSDQSSGKCEDYRVMLTCPMDFCQVCRTGWFDIDDPSGRGDYETLQQILVEHPEGVCSDPLGIEAMTTAGTPALQTNNVFQVYDATYGFACVNAEQPPNKPCEDYKVRFTCPPQFCEHRRPPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M137</accession>
    <name>W5M137_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Insulin-like growth factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Insulin-like growth factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642516.1">
        <property type="nucleotide sequence ID" value="XM_006642453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M137"/>
    <dbReference type="FunCoup" id="W5M137">
        <property type="interactions" value="1599"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002094"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002099.1">
        <property type="protein sequence ID" value="ENSLOCP00000002094.1"/>
        <property type="gene ID" value="ENSLOCG00000001806.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684037"/>
    <dbReference type="KEGG" id="loc:102684037"/>
    <dbReference type="CTD" id="324215"/>
    <dbReference type="eggNOG" id="ENOG502S0I0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160745"/>
    <dbReference type="HOGENOM" id="CLU_092464_1_0_1"/>
    <dbReference type="InParanoid" id="W5M137"/>
    <dbReference type="OMA" id="KVQRMCA"/>
    <dbReference type="OrthoDB" id="9449995at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001806">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005159">
        <property type="term" value="F:insulin-like growth factor receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042104">
        <property type="term" value="P:positive regulation of activated T cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046628">
        <property type="term" value="P:positive regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="INSLNLIKEGF2"/>
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    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
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        <property type="entry name" value="INSULIN"/>
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    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="3 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="47"/>
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    <feature type="domain" description="Insulin-like" evidence="12">
        <location>
            <begin position="57"/>
            <end position="113"/>
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        <location>
            <begin position="60"/>
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            <begin position="72"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
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            <begin position="99"/>
            <end position="104"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00023030"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049761"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049823"/>
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        <source>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000406"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="24925" checksum="48C84FC5F1CA691B" modified="2014-04-16" version="1">MEDQQKYSYQAFCHTCLGTENRRMKMRKMSTSRQMLVFTIALTFYIMDVAKPFSYAETLCGGELVDALQFVCEDRGFYFSRPTSRSNSRRAQKGIVEECCFRSCDLNLLEMYCAKPAKSERDVSSTSLQGIPALPALKEAPRKPLTVKYSKYDVWQRKAAQRLRRGIPAILRARKFRHQAEKVKAQEQLLFHKPLITLPSKLPPAVQSSTEKSVSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M264</accession>
    <name>W5M264_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">THO complex 1</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000002472.1"/>
        <property type="gene ID" value="ENSLOCG00000002097.1"/>
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        <property type="component" value="Linkage group LG9"/>
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        <property type="term" value="C:THO complex part of transcription export complex"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Death Domain, Fas"/>
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        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
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        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
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            <dbReference type="PROSITE" id="PS50017"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="656" mass="75590" checksum="0DBCBFBB17618CFC" modified="2014-04-16" version="1">EKMSPSVFNFNDAKERFASTIRSTLDSKTVKPLINTFNQLPGNETEKKTTLDQALRWVLEGEIIKRKASCEDFLALIYLSIDGVTEGICSATTPFLLLGDVLDCLPLDQCDKIFTFVEENVSTWKSNSFYSAGKNYLLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSAFLGLNLQSQFNLDNYTVFNKNEQESTLGQKHTEEKDEGMEVEEGEMGDEEAPTPCSVPIDYNLYRKFWTLQDYFRNPVQCYDKFSWMTFLKFSDETLAVFKSYKLDDTQASKKKMEELRTSGGEHVYFAKFLTSERLMDLQLSDSNFRRHILVQYLVLFQYLKGQVKFKSSSCVLTDEQSLWMEDTTKLVYQLLRETPPDGDKFASMVEHILNTEENWNAWKNEGCPSFVKERPAEMKPIRPTRKRQAPEDFLGKGPDRKIFMGNDELTRLWNLNQDNMEACKSDSREFMPSLEEFFEEAIEQADPANMVEDEYKVVKNSNYGWRALRLLARRSPHFFQPTNQQFKNLPDYLENMVIKLAKELPPPSEEIKTGEEDDDNDDALLKESNESPDVQHKLVTNQQMDDIASKLGAQWKTLAGQLEMKAVEVREIETDSEDVEMQAKLLLVAWQDREGSQATVENLVLGLNAAGFIKIAESLTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3G8</accession>
    <name>W5M3G8_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3G8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002926"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002932.1">
        <property type="protein sequence ID" value="ENSLOCP00000002926.1"/>
        <property type="gene ID" value="ENSLOCG00000002497.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166741"/>
    <dbReference type="HOGENOM" id="CLU_409903_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3G8"/>
    <dbReference type="OMA" id="TNSGSKM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002497">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052231">
        <property type="entry name" value="Rho_GEF_signaling-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING, FAMILY G (WITH RHOGEF DOMAIN) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45845">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="670" mass="70793" checksum="EB11BEA6336B9A4B" modified="2014-04-16" version="1">MGPRDFYLQVVPFSTRSPRLALKCLSSGGCTVQEVLIPETQHAHVFTPEWLNGINKERGGGRRLETCLVTTGEGLVKVPWEDIVYPRFVPEPECPLGQGPVPNPAPPRSQGKDANSVPGRDSDNEGESQPASGGVPPVAKNATDQDKHLETLGEEEIPSPTRGEVPKEEEAGGDYVELLEFQGGTDVGGRVGSDSKRRYLEMHGICKTKTVPLCKKGKGGKGRRAKAWVHCRNEMAGGMKKTSSSSSSSSVGGHSGKGDSGSSGEELSRPALDMISLEREEKPDHSSHLSGKKWPVGSSGKGETDPGKEGDTGDGNGLLGSRDSPDGRANKNCEESGGDGEGMQTKEQSATQGTNHSTGTPEKDQSEETSGSSQVSDLMTESPQNKSETEGDQSLPGKSSADSLPTSTSKSASLPGQTNSQCHSSSGPGQDSQVAVHYGEAEEKAIPPRIKTGKAVGSRAHRRKRRVKGGRGKSKSSGQSRKADVKESTPLSEPKAPSPTPQQPELKAQPTGSTQEPPSNPPPPTPDSQSEPVQSPGVKGEGSQLSNARRDQTEYDRTASDRDAESSTAKEPEIPPPQGDSQPRAASPDHSQGNVEQNRLPDQSGGSSCQAPPLLRDLNPELLQSGMLILPGTVDRMGRALVVMEARSQGWGFRDDEVASALACFHGLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3U1</accession>
    <name>W5M3U1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3U1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003049"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003055.1">
        <property type="protein sequence ID" value="ENSLOCP00000003049.1"/>
        <property type="gene ID" value="ENSLOCG00000002597.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004550"/>
    <dbReference type="HOGENOM" id="CLU_017316_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3U1"/>
    <dbReference type="OMA" id="ALCHQDI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002597">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45153:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="235" mass="26569" checksum="C3C29731C29C6BEF" modified="2014-04-16" version="1">MCLLSLSSQGQCLFDQGLYLDALESFAKASELIPDNRSYHMRSLACLAALGQHGDCLRLASSWLETDASNPDLFVLRARLHRHFSQATLCYRDVKAALAVAPEFQEARRLLSELRGVAEGARERAVSRAVGGQLREALQKINTAVEHDPEQSHYYLFRATLHRRLRDFNAAIDDLIEVLGRGGGEQAEARRQLALTYNDFAVHCFSRRFYQEAELLLSKAIEEEKREKGLYINRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M469</accession>
    <name>W5M469_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">Neuromodulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17 20">Axonal membrane protein GAP-43</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 20">Growth-associated protein 43</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16 20">This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Cell membrane</location>
            <topology evidence="20">Peripheral membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Cell projection</location>
            <location evidence="1 20">Growth cone membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19 20">Synapse</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Cell projection</location>
            <location evidence="20">Filopodium membrane</location>
            <topology evidence="20">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="20">Palmitoylated. Palmitoylation is essential for plasma membrane association.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the neuromodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196612.1">
        <property type="nucleotide sequence ID" value="XM_015341126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M469"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003177"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003184.1">
        <property type="protein sequence ID" value="ENSLOCP00000003177.1"/>
        <property type="gene ID" value="ENSLOCG00000002709.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691988"/>
    <dbReference type="KEGG" id="loc:102691988"/>
    <dbReference type="CTD" id="2596"/>
    <dbReference type="eggNOG" id="ENOG502RXWF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111265"/>
    <dbReference type="HOGENOM" id="CLU_102989_0_0_1"/>
    <dbReference type="InParanoid" id="W5M469"/>
    <dbReference type="OrthoDB" id="9397439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002709">
        <property type="expression patterns" value="Expressed in brain and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035727">
        <property type="term" value="F:lysophosphatidic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016198">
        <property type="term" value="P:axon choice point recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031103">
        <property type="term" value="P:axon regeneration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001422">
        <property type="entry name" value="Neuromodulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018947">
        <property type="entry name" value="Neuromodulin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033137">
        <property type="entry name" value="Neuromodulin_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018243">
        <property type="entry name" value="Neuromodulin_palmitoyl_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10580">
        <property type="entry name" value="Neuromodulin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00215">
        <property type="entry name" value="NEUROMODULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00412">
        <property type="entry name" value="NEUROMODULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00413">
        <property type="entry name" value="NEUROMODULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4 20" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 20" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 20" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="15 20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13 20" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Neuromodulin N-terminal" evidence="22">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="213"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU368113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF10580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="244" mass="25929" checksum="95DD7FAC1F1C846C" modified="2014-04-16" version="1">MLCCIRRTKQVEKNEEADQKIEQDGNKPEDKAHKAATKIQASFRGHIIRKKLKDEKKEDGTAAPEAAAAADGVENKEEKASSPAAESKEPAAEAVDEPSTANAEAKREEADAVETSKDEAKPAAATDPSEKAKDVASPEEKAAAATEGENKEAPPAQESATTTSASTESAQSPNAVEPAEKKEPQQADVPAADASETTPKVQSEETDSNQPQDKQDAVEESKPAESDHTEETKVEDIKADQENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M485</accession>
    <name>W5M485_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPCR family 3 nine cysteines domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01022561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M485"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003193"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003200.1">
        <property type="protein sequence ID" value="ENSLOCP00000003193.1"/>
        <property type="gene ID" value="ENSLOCG00000002717.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="HOGENOM" id="CLU_1365862_0_0_1"/>
    <dbReference type="InParanoid" id="W5M485"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000007">
        <property type="entry name" value="Vomeronasal 2, receptor 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GPCR family 3 nine cysteines" evidence="1">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="200" mass="22762" checksum="C5E8938B112906F6" modified="2014-04-16" version="1">MATFLQAAQQEGICIEYSEAIYRTDPREKFLKVVDIVKKSTSNVQHYLKRVNYTTQKGEKVFFDDNGDPVARYAIVNWQPNNEGITTFKIIGFYDGSFPEGQQFIMNNISPVWAGHQYKIPKSVCSESCLPGTRKAIEKGKPVCCFVCIPCAEGEFSNTTGRSRLLCVCHSELYTFFCFSNNEWNCPKLYILVPKMLCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M501</accession>
    <name>W5M501_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS38</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mS38 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642074.1">
        <property type="nucleotide sequence ID" value="XM_006642011.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192731.1">
        <property type="nucleotide sequence ID" value="XM_015337245.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192732.1">
        <property type="nucleotide sequence ID" value="XM_015337246.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M501"/>
    <dbReference type="FunCoup" id="W5M501">
        <property type="interactions" value="1289"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003459"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003467.1">
        <property type="protein sequence ID" value="ENSLOCP00000003459.1"/>
        <property type="gene ID" value="ENSLOCG00000002935.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695701"/>
    <dbReference type="KEGG" id="loc:102695701"/>
    <dbReference type="CTD" id="54998"/>
    <dbReference type="eggNOG" id="ENOG502S2YD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012802"/>
    <dbReference type="HOGENOM" id="CLU_112940_0_0_1"/>
    <dbReference type="InParanoid" id="W5M501"/>
    <dbReference type="OMA" id="GWTTPKI"/>
    <dbReference type="OrthoDB" id="6139741at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002935">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23699">
        <property type="entry name" value="At5g63150_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013177">
        <property type="entry name" value="Ribosomal_mS38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035">
        <property type="entry name" value="AURORA KINASE A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08213">
        <property type="entry name" value="COX24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01155">
        <property type="entry name" value="DUF1713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein mS38 C-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="23932" checksum="A2CF84CB360BDBCD" modified="2014-04-16" version="1">MFVSRLALRLRASCTVSGCLQIPGRFGGIEGVNPPCRLPPCGRARYSSKAAVDSAHSPQRWITLSPELEELLVPRKLAVSPLESWLSLRYSLPSLGGSTDPQPLEEGVGESEPKALPPSGNPTPGADEGIRPLSCKNVLEIRRRKMNRHKYKKLLKRTKFLRRRVIEGRRKRKQGRFERDLKRIWVRAGLKKAPEGWTTPKIYIKQYQGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5I9</accession>
    <name>W5M5I9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="6">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01010575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5I9"/>
    <dbReference type="FunCoup" id="W5M5I9">
        <property type="interactions" value="1217"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003647"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003654.1">
        <property type="protein sequence ID" value="ENSLOCP00000003647.1"/>
        <property type="gene ID" value="ENSLOCG00000003079.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160726"/>
    <dbReference type="HOGENOM" id="CLU_002173_0_1_1"/>
    <dbReference type="InParanoid" id="W5M5I9"/>
    <dbReference type="OMA" id="GWCEGSS"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003079">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04021">
        <property type="entry name" value="C2_E3_ubiquitin_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000023">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000082">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF396">
        <property type="entry name" value="NEDD4-LIKE E3 UBIQUITIN-PROTEIN LIGASE WWP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="420"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="12">
        <location>
            <begin position="553"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7 8">
        <location>
            <position position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="887" mass="100411" checksum="DA1577C59DCA8DDA" modified="2014-04-16" version="1">MASASSSRSGVGQSSEKSQLTLKVLSAKPQSPKSPSRHSRLNSFVEVTADGLSSETKKTGKRCGHPELQWNEDLTLNVTPQSHLDLKLWSCHALRNELLGIASIDVYDTLKSNNGKMENVQLNLALQTENKGSLVPGGELTVCLDGLAVDLGSLPNGSAVTDKPQQSDGHGIRRTQSEDSPPSSSGSCSGPRGRSHRHSAGMARGGPVANGDPAVNGEHSPGARSRRRQTGKALSSNTGLGTPGKAEVAANGTEDGRRSPTCQTPPPSAALSTSTTPCAGTPSSGSDAPQGDTGETGGTPTPDQASAAPEQNMESLPPGWEQRVLPNGRIYYVDHNTKTTTWERPLPPGWEKRVDQRGRFYYVDHNTRTTTWQRPTAESVRNYEQWQSQRSQLQGAMQQFSQRFLYQSIGMRALQRTKLSLLNSGWYEKRQDNGRVYFVNHNTRTTQWEDPRTQGMIQEPPLPPGWEMKYTNEGVRYFVDHNSRTTTFKDPRPGFDSGSKQGGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKKPTLKDLESIDPEFHNSIMWVKENNLEECGVELYFAQDMEILGKVSTHELKTDGENELVTEDNKEEYIRLLTDWRFTRGVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLTDWQKNTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFSELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSFEQLKEKLLFAIEETEGFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6C2</accession>
    <name>W5M6C2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Synaptonemal complex protein 2-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SYCP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6C2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003930"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003938.1">
        <property type="protein sequence ID" value="ENSLOCP00000003930.1"/>
        <property type="gene ID" value="ENSLOCG00000003325.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVM5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063859"/>
    <dbReference type="HOGENOM" id="CLU_904759_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6C2"/>
    <dbReference type="OMA" id="SAMLCEH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003325">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024835">
        <property type="entry name" value="SYCP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041322">
        <property type="entry name" value="SYCP2_ARLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040560">
        <property type="entry name" value="SYCP2_SLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607:SF12">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18581">
        <property type="entry name" value="SYCP2_ARLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18584">
        <property type="entry name" value="SYCP2_SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synaptonemal complex protein 2 armadillo-repeat-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Synaptonemal complex protein 2 Spt16M-like" evidence="7">
        <location>
            <begin position="180"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36527" checksum="DDDD0702E0F6107C" modified="2014-04-16" version="1">MVQWFDKAREIWAEGGHIKNENLLNFAEDFFDFVMAVHENSNEGKSQVTKTFLHRAGQLTADAGVHISIQQEAVRKLNQILDESPHELKRQVLSSAEILSVMKLLGKRIMEGGDYDLQVALTEALCRMTTEKQRHELADGWFSVEFVAKAFKRIKDSEFETDCRKFLNLINGMQGDRRRVYSYPCLEVFLDKHELFMPADDKLEEFWIDFNLGSQSITFYISTADGEEENQWDTVCIPKDEVEYYTVKEEKGQKVLTVILKQLLNSGGREGNRIQMYFSAALDIGEAVRHVYGEPENKVSLTPKSLSIQLYTGFQSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M795</accession>
    <name>W5M795_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NCK-associated protein 5-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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        <property type="gene ID" value="ENSLOCG00000003584.1"/>
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        <property type="component" value="Linkage group LG4"/>
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    </dbReference>
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        <property type="term" value="C:microtubule plus-end"/>
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        <property type="entry name" value="NCKAP5_C"/>
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    <dbReference type="InterPro" id="IPR026163">
        <property type="entry name" value="Nckap5l"/>
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        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5"/>
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        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5-LIKE"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="1069"/>
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            <begin position="949"/>
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        <location>
            <begin position="1441"/>
            <end position="1535"/>
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        <location>
            <begin position="33"/>
            <end position="99"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1155"/>
            <end position="1182"/>
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            <begin position="15"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="1535" mass="167636" checksum="3F3D512DB153CA0B" modified="2014-04-16" version="1">MSEEAEPRPCSDAFDPGEGEEEPALEGLEAESSRELLERLRELEAENSALALANESQREAYERCLDEVANHVVQALLNQKDLREECIKLKMRVFDLERQNRTLSELFHQKLHTTAGSLQQVGLCFAKHGLLQQGRYYRPVETPAQAQTKSAGDCARNGTPGTRAPAASMDALSPFFRKKAHILEVLRKLEETDPLKFHPSAGLSSYRDIGQALLPTDPIRPGEHAVQRKASDMERHLSGDSDIHDYMNGEGALPEDNGLDYESCQSCLVFSQKSLDSLFRWKQDNAMGPTKRENTRGLDHKDAPVSIQTKESMMILPPGNANECCSPAEKTSSDFPSEPRNEKPNDLAQLLASKSYFCSLIKDTDQNGLNKQQEDSLSLEEHNNGPCEEKCCGKLSGANLPLPCQSPPRENDDLPSKQNLKHIKTVAFPSLNAGEVINTEEHYLKLEAYSSTEATNVKNTYCQQESREQMCNGLYFSESCFSKEVDSYLPASTPEKNDLNFLQQVTQEKGKLALSPTSPSCMTDTKPSPISSPSRLLKFLKIPSIGERAQASNPLRLSPQLTRSSKIPCRNNNYEVYHSPATARRAATAERDKQPAPSKNDSYPSTHSAPTSPPKPEEVLPLQKDPSYSSHSAPKASRNAKLAPSGQQQRSFQKVPHYENLSDLPTACQADGSTQVLERVNQSSFPSCSPEGHSEKQVNSLEEKRISPDHKEHHLPCGSSSSSTNPSCDPAAPDQDLYVENSVWYKIHDHHSLPNSSAVHKSQNSCLYSSMKDKHVPEAALQASDSIQSFNLPVTAKKGEPPSIPKKPVSGKSPSESSHLPFKERLAALGKLKNTEDSHTTGPFVGSPLDKKDGQCNGRVVSSSNNERSKTAERQCDRNGADHRHSKYTDSLDGKPYPKANLNSYGLKAPGPYEPGVKSLPPSTSTPKADLETFSSRIYVARADVPKIRAALPSSNPDPPQVLRNFMKCANAQNPSQNGKTAPSPQGSPTKVPFKSPTKPNQGSSSSSRGSKPTQDERVTSQKHSPRADDKSKLLANKKKNSAYSDSLPPPPPRPDAVGDKRPLVPGPQSAIEQKVMKGIEENMLKLQVQDRGQVVEVKQKTSNGIASWFGLKKSKLPALSRKADVAKLKEDKKEWKLNMPSAGKDPKVATKKKLEVESLNISKLMEKAEDLRKALEEERAYVNGVALDRASRGHSCEVVMDQNQSQLSVVYREVTSDTFMQQLLNRVDERDSSGNGLAHRRLSFDSKKSRPIFSSQRNGIISHAKSRQEVAKATDLVCKGDITSDESLTESVNSQHFAGSGSSTRTLDSGIGTFPLPDYAGCVAGKLVPKVKPRGDQEFSAPLGKAGPVTKVPRKARTLERELSSLEEAFPRDKDLSPGLYSAALEGKGSSTHLSTAIQEDVDAYGAHVQSPPSKNWTFPNLKASSGPTEVYLGMRSDVEPASHGTPFRRSVKPCAAAPSRDSDPASLALPPPLGRRAKSRTLPGPEMGKEGGLELVKEKADDVLSPGRPPALETPESLSDSLYDSLSSCGSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7N7</accession>
    <name>W5M7N7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11 12">Cytoplasmic vesicle</location>
            <location evidence="11 12">COPI-coated vesicle membrane</location>
            <topology evidence="11 12">Peripheral membrane protein</topology>
            <orientation evidence="11 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11 12">Golgi apparatus membrane</location>
            <topology evidence="11 12">Peripheral membrane protein</topology>
            <orientation evidence="11 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024175">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7N7"/>
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        <property type="interactions" value="1993"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004395"/>
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        <property type="protein sequence ID" value="ENSLOCP00000004395.1"/>
        <property type="gene ID" value="ENSLOCG00000003694.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017207"/>
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        <property type="component" value="Linkage group LG26"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="14">
        <location>
            <begin position="152"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="382" mass="42980" checksum="A7E35CE487B665C4" modified="2014-04-16" version="1">MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPLEKLYMVLITTKNSNILEDLETLRLFSRVIPEYCRVLEESEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHEEKVFRAVRETQEREAKAEMRRKAKELQQARRDAERSGKKAPGFGGFGSSGMSGSSPITITDTLIEPEKPKATPAPSRYPRSQPAWTHCLSDYSNRTGRRHLPLLCPPAVNCWPSESGSGCDVNIEYELQEEGLELNDVVISIPVPSGVGAPLIGDLDGEYRHDSRRNVLEWCLPVIDAKNKTGSLEFSIAGQPNDFFPVHVSFVSKRNYCDIQISKVTLVDSDSPVRFSLETSFVVDKYEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M845</accession>
    <name>W5M845_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TYRO protein tyrosine kinase-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DNAX-activation protein 12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYROBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M845"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004553"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004561.1">
        <property type="protein sequence ID" value="ENSLOCP00000004553.1"/>
        <property type="gene ID" value="ENSLOCG00000003818.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SCVI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198998"/>
    <dbReference type="HOGENOM" id="CLU_2108156_0_0_1"/>
    <dbReference type="InParanoid" id="W5M845"/>
    <dbReference type="OMA" id="QRQPYYK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003818">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002282">
        <property type="term" value="P:microglial cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030889">
        <property type="term" value="P:negative regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032911">
        <property type="term" value="P:negative regulation of transforming growth factor beta1 production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002283">
        <property type="term" value="P:neutrophil activation involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034241">
        <property type="term" value="P:positive regulation of macrophage fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904151">
        <property type="term" value="P:positive regulation of microglial cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032816">
        <property type="term" value="P:positive regulation of natural killer cell activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026200">
        <property type="entry name" value="Tyrobp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17554">
        <property type="entry name" value="TYRO PROTEIN TYROSINE KINASE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17554:SF2">
        <property type="entry name" value="TYRO PROTEIN TYROSINE KINASE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867759" description="TYRO protein tyrosine kinase-binding protein" evidence="16">
        <location>
            <begin position="17"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="115" mass="12795" checksum="EED3603C653EAD70" modified="2014-04-16" version="1">INWYLFLFFPPGPVVGQSFSISDCQSCYHLASGTIVGIIVGDIILTLLIALSVFCFVSRMKKNHLEALQGQCMKATKGNISMSASRKVKDDAESTYQDLQGVRNDIYSDLRRVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8N6</accession>
    <name>W5M8N6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Stress-activated protein kinase JNK</fullName>
            <ecNumber evidence="13">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. May play a role in the regulation of the circadian clock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01025228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015201900.1">
        <property type="nucleotide sequence ID" value="XM_015346414.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8N6"/>
    <dbReference type="FunCoup" id="W5M8N6">
        <property type="interactions" value="693"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004744"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004752.1">
        <property type="protein sequence ID" value="ENSLOCP00000004744.1"/>
        <property type="gene ID" value="ENSLOCG00000003968.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692400"/>
    <dbReference type="CTD" id="562720"/>
    <dbReference type="eggNOG" id="KOG0665">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153692"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="W5M8N6"/>
    <dbReference type="OMA" id="AMDMWAV"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003968">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004705">
        <property type="term" value="F:JUN kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07850">
        <property type="entry name" value="STKc_JNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000009">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000210">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008351">
        <property type="entry name" value="MAPK_JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01772">
        <property type="entry name" value="JNKMAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="384" mass="44147" checksum="D33C5A62DEEBB3AA" modified="2014-04-16" version="1">MNRNKREKEYYSLDVGDSTFTVLKRYQNLRPIGSGAQGIVCSAYDHILERSVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYLVMELMDANLCQVIQMELDHERLSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAATGLLMTPYVVTRYYRAPEVILGMGYQANVDVWSIGCIMAEMVRGSVLFPGTDHIDQWNKVIEQLGTPSQEFLMKLNQSVRTYVENRPRYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPAEVEAPPPLITDKQLDEREHAVEEWKELIYKEVLEWEERLKNGVVRGQPASLAQVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8T4</accession>
    <name>W5M8T4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Insulin-like growth factor binding protein 6b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199529.1">
        <property type="nucleotide sequence ID" value="XM_015344043.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8T4"/>
    <dbReference type="FunCoup" id="W5M8T4">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004793"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004801.1">
        <property type="protein sequence ID" value="ENSLOCP00000004793.1"/>
        <property type="gene ID" value="ENSLOCG00000004011.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687336"/>
    <dbReference type="KEGG" id="loc:102687336"/>
    <dbReference type="CTD" id="100301946"/>
    <dbReference type="eggNOG" id="ENOG502QV3Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160528"/>
    <dbReference type="InParanoid" id="W5M8T4"/>
    <dbReference type="OMA" id="CARPGRN"/>
    <dbReference type="OrthoDB" id="8875634at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004011">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031995">
        <property type="term" value="F:insulin-like growth factor II binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043567">
        <property type="term" value="P:regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000005">
        <property type="entry name" value="Insulin-like growth factor-binding protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022326">
        <property type="entry name" value="IGFBP-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022321">
        <property type="entry name" value="IGFBP_1-6_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551:SF27">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 6A PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01976">
        <property type="entry name" value="IGFBPFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01982">
        <property type="entry name" value="IGFBPFAMILY6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865861" evidence="7">
        <location>
            <begin position="26"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="8">
        <location>
            <begin position="142"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="219" mass="23082" checksum="BBCAE76F9AA33103" modified="2014-04-16" version="1">MPTPPSLQALLTLLVALLCQPGTRALGAHLVPTRGCPSCRQEGGPPRALREPGGGETGTAVLALGEPCGVYTLGCARGLRCVPRPGEKSPLQALLQGRGVCARPGRNKTGHNPVLKNLTTAVLPGGSSVPPARSPDGRSAELAPCRRLLNSVLESLKLTVIQSPKDIYIPNCDTRGFYRKNQCRSSKGTRRGECWCVDELGQPLTSPDRGDASLQCDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9T6</accession>
    <name>W5M9T6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tumor necrosis factor receptor superfamily member 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Apo-1 antigen</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Apoptosis-mediating surface antigen FAS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">FASLG receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9T6"/>
    <dbReference type="FunCoup" id="W5M9T6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005145"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005153.1">
        <property type="protein sequence ID" value="ENSLOCP00000005145.1"/>
        <property type="gene ID" value="ENSLOCG00000004304.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S19V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159540"/>
    <dbReference type="HOGENOM" id="CLU_621045_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9T6"/>
    <dbReference type="OMA" id="GVFWVQV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004304">
        <property type="expression patterns" value="Expressed in mesonephros and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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            <begin position="18"/>
            <end position="441"/>
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            <begin position="58"/>
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            <begin position="73"/>
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    <feature type="disulfide bond" evidence="16">
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            <begin position="141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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            <dbReference type="ARBA" id="ARBA00004285"/>
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            <dbReference type="ARBA" id="ARBA00015761"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="441" mass="50170" checksum="0A659A69524ABA8F" modified="2014-04-16" version="1">MKLSGWFCLWLLPLTLASHTWKESAPYSTAQWTSATLARTSRTTHRKGNGRVRRHTVSCGEHQFLDREANRCCNKCLPGHYKQSSCSDNTNQTRCAPCKEGSFMDQYNHVLTCHRCRSCDHAKLEEVKKSCNSTSNTVCGCKKGSYRKNDSQDFPCAKCSLCHNRTIKENCSENADTQCGDCVPGLYQDKHGECQPCRNCSVIQREREHCHSFSDQVADCRSCRLTFFLEFTCLLKSTNCSSFVGICKRKYRDRGNFILRCSIDSRYKLQRFGRTAQRTTKTHLQADEKNNLVTVKLWPTVKAQLRSMPGAVAAKEEPPADAWPPAVLYAIIEEVPVRRWKEFMRVLALPDGEMERVELELAPSYRERQYQMLRLWSQRRGAELQSVHAALHSMDLPGCSQELQEKLVFLMTPKPTAPALSYPSLIQYRPEAGEASAGPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAD0</accession>
    <name>W5MAD0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CLU family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="1323"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005339"/>
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        <property type="protein sequence ID" value="ENSLOCP00000005339.1"/>
        <property type="gene ID" value="ENSLOCG00000004458.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012485"/>
    <dbReference type="HOGENOM" id="CLU_003256_1_0_1"/>
    <dbReference type="InParanoid" id="W5MAD0"/>
    <dbReference type="OMA" id="HPVWDKD"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004458">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000088">
        <property type="entry name" value="Clustered mitochondria (CluA/CLU1) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CLU-central"/>
    </dbReference>
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        <property type="entry name" value="CLU_dom"/>
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        <property type="entry name" value="CLU_N"/>
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        <property type="entry name" value="CLU_prot"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
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    </dbReference>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
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    </dbReference>
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        <property type="entry name" value="CLU"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="6">
        <location>
            <begin position="374"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="676"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1338"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="731"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1371"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1386" mass="154276" checksum="B21D6220C9B53E23" modified="2014-04-16" version="1">MVSKTDDIPAAVPNKTGELTDGETHRGDVARKDSRGCAGHAVDTSVMNGDGAHEQTEECKQNGGGDLDQAEDANEQEVIVIQDTGFTVKVQAPGTEPFDLQVSPQEMVQEIHQVLMDREDTCHRTCFSLQLDGNVLDNFAELKSIEGLQEGSVLKVVEEPYTVREARIHVRHIRDLLKSLDPSDAYNGVDCNSLSFLSAFTEGDLGESGKRKKKGSELEQIDCTPPEHILPGSKDRPLSPLQPQSKDWKPLQCLKVLTMSGWNPPPGNRKMHGDLMYLYVVTVEERHVSITASTRGFYLNQSTTYIFNPKPANPSFLSHSLVELLSQISPAFKKNFTALQKKRVQRHPFERIATPFQVYSWTSPQVDHAMDCVRAEDAYTSRLGYEEHIPGQTRDWNEELQTTRELPRKNLPERLLRERAIFKVHSDFAAAATRGAMAVIDGNVMAINPGEETRMQMFIWNNIFFSLGFDVRDHYRELGGDSAAHAAPTNDLNGVRAYGAVDVEGLYTLGTVVVDYRGYRVTAQSIIPGILEREQEQSVIYGSIDFGKTVVSHPKYLELLEKTSRPLKIQRHAVLNEKAEAAELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVEGEELPPECRRLGFPRQHRHRLACLRQELIEAFVEHRYLLFMKMAALQLMQQKASRDCGGSVPPALVANGDLESSPSQDPPSQDGDEAAVPTATAANGPLPQALGQPENGECETPRSSEGKGAEENIPGLAQAKELAEAVAADDGTADAKSRDVVLNACKAVGSISNTSFDIRFNPDIFSPGVRFPEDSLEDVQKQKQLLKDAAAFLVSCQIPSLVKDFLDHSALPMDGATLTEALHQHGINVRYLGNVLDFIDKTPARAQLDHIYKIGIGELITRCAKHVFKTYLQGVELSALSAAVSHFLNCCLSSFPDAVAHLPADELVSKRKNRRRRNRVPGGGSGDNTAWASLTPSELWKNIASEAQSYYHFTLECENVDQAVDKYGLQKITLLREISVKTGFQILIKEYNFDSRHKPAFTEEDILNIFPVVKHVNPKASDAFHFFQSGQAKVQQGFLKEGCELINEALNLFNNVYGAMHVEICACLRLLARLNYIMGDYPEALSNQQKAVLMSERVLGIEHPNTIQEYMHLSLYCFANGQLSTALKLLYRARYLMLLVCGEDHPEMALLDSNIGLVLHGVMEYDLSLRFLENALAINSKYHGTRSLKVALSHHLVARVYESKAEFRSALQHEKEGYTIYKNQVGEAHEKTRESSEYLKYLTQQAVALQRTMNEIYKNGSNASIMPLKFTAPSMASVLEQLNIINGIIFIPLSQKDLENLKAEVQRRQQLQDSGKSEEQQESSQPELENKLTAEKMQSAPSAQEATAPQQPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBE8</accession>
    <name>W5MBE8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcium-activated potassium channel subunit alpha-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">BK channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">BKCA alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Calcium-activated potassium channel, subfamily M subunit alpha-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">K(VCA)alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">KCa1.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Maxi K channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Slo-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Slo1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Slowpoke homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005707"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005715.1">
        <property type="protein sequence ID" value="ENSLOCP00000005707.1"/>
        <property type="gene ID" value="ENSLOCG00000004752.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154935"/>
    <dbReference type="InParanoid" id="W5MBE8"/>
    <dbReference type="OMA" id="NINACDM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004752">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000098">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="30">
        <location>
            <begin position="379"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="30">
        <location>
            <begin position="814"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="732"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1188"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="1191"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="1222"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1290" mass="144867" checksum="87784E52144AE801" modified="2014-04-16" version="1">MLAMADGNVSEGISSSMSNNINNNINPDSSSVYMSKMDAVIIPYSADVPCDSNGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKHKEAQKINSNAGNQADGSAKGPDEKEETPASEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSDPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNSQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPTVCELCYVKLKLLLIAIEYKSDKRESSILINPGNHVKMQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLIYSKMSIYKRMKLACCFDCGRSERDCSCMSGSVHSNMDTLERAFPLSSVSVNDCSTSLRAFEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRHDPLLIPGNEQIENMDVSVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALVGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLVRQASVTTGANIPIITELAKPGKLLPLVSLSQEKNSGTNIQMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRTSLSHSSHSSHSSSKKSSSVHSIPATNRQNRSKARDSRERQNATRMNRMGQEKKWFTDEPENAYPRNIQIKPMSTHMVNQVNQYKSTSSLIPPIREVEDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCH7</accession>
    <name>W5MCH7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015223736.1">
        <property type="nucleotide sequence ID" value="XM_015368250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCH7"/>
    <dbReference type="FunCoup" id="W5MCH7">
        <property type="interactions" value="1737"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006094.1">
        <property type="protein sequence ID" value="ENSLOCP00000006086.1"/>
        <property type="gene ID" value="ENSLOCG00000005055.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684034"/>
    <dbReference type="CTD" id="1783"/>
    <dbReference type="eggNOG" id="KOG3905">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008295"/>
    <dbReference type="HOGENOM" id="CLU_021937_2_1_1"/>
    <dbReference type="InParanoid" id="W5MCH7"/>
    <dbReference type="OMA" id="FKHNVID"/>
    <dbReference type="OrthoDB" id="27603at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005055">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090235">
        <property type="term" value="P:regulation of metaphase plate congression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009794">
        <property type="term" value="P:regulation of mitotic cell cycle, embryonic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF1">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 LIGHT INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="367"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="460" mass="50883" checksum="C35B7C9229593807" modified="2014-04-16" version="1">MAPVLEKKLLGAAGAADTLEINNEDEEGQNLWSSILSEVSTRSRSKLPSGKNILVFGEDGSGKTTIMAKLQGAEHNKKGRGLEYLYLNVHDEDRDDQTRCNVWILDGDLYHKGLLKFAVSAESLKDTLAVFVADMSRPWTIMESIQKWASVLREHIDKLKIPPEQMRELEQKMVKDFQEYTEPEEATQGSPQRRGPTASGTDDEGVLLPLGDNVLTHNLGIPVLVVCTKCDAVSVLEKEHDYREEHFDFIQSHIRRFCLQYGAALIYTSVKEEKNLDLLYKYIVHKMYDFQFTTPALVVEKDAVFIPSGWDNEKKIAILHENFTTVKPEDPFEDFIMKPPVRKLVHDKEVAAEDEQVFLMKQQSLLAKQPATPTRATESPARAPSGSPRPTGRTGPANVASVSPMTAVKKPDPNMKGAAANEGVLANFFNSLLSKKTGSPGSPGAGGVQSTAKKAEAGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCL1</accession>
    <name>W5MCL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01018355">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCL1"/>
    <dbReference type="FunCoup" id="W5MCL1">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006120"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006128.1">
        <property type="protein sequence ID" value="ENSLOCP00000006120.1"/>
        <property type="gene ID" value="ENSLOCG00000005077.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154339"/>
    <dbReference type="HOGENOM" id="CLU_000288_132_1_1"/>
    <dbReference type="InParanoid" id="W5MCL1"/>
    <dbReference type="OMA" id="PEDVTXD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005077">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08687">
        <property type="entry name" value="C2_PKN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000003">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037784">
        <property type="entry name" value="C2_PKN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 25 27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="31">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="31">
        <location>
            <begin position="112"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="31">
        <location>
            <begin position="198"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="650"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="30">
        <location>
            <begin position="910"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="343"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="525"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="40"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="23">
        <location>
            <position position="775"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="780"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="793"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
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            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
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            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="977" mass="110704" checksum="951ACD7B408C0E4F" modified="2014-04-16" version="1">MAADSVQGDARSHLVSERLGLGHNLDLSDTMVQQKLDEIKDQIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNMLKKSNKKVEELHQELQELNAHIVVKDPEELLDCPRTPDTPNSEVRTSTNSSRLAALKKQNDIELKVKQGAENMILMYSNGSSKDRKLLATAQQMLQDSKTKIEFIRMQILKASQTSEVNFENSDGLAKPIISPLELRLEELRHHLRIEHAVAEGAKNVMRLLGSGKLTERKAHSEAQARFNESSQKLDLLKYSLEQRLNELPKNHPKSSLIMEELSLVASPALSPRQSIISTQNQYSTVAKPAALTGTLDVRLMGCQDLLEVVPGRSKASSVPLPGWSPSENRSSFMSRGSKNKGGSSRNLSKTDDLSNEISAVLKLDNTVVGQTSWKPISNQAWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFPRAPQMNINIATWGRLMRRAIPSVNNSSAFSPQVSELSSESPSTTQPSSPTSPSDQTVMKLDFDREPTPTPKLQSTAVETREPLPESKIPDRQVQDALATFDFLNDRNSIVVKPDSEGLEEHEVRQPDLELITDPSTDVRGQQQFHSGLSDFKCVAVLGRGHFGKVLLAEYKNTGEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHADVFSEPRAVFYAACVVLGLQFLHDHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFRDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGERDAEEVKKHPFFRIVDWTALLAKKARPPFVPTIRGREDVSNFDDEFTSEAPVLTPPREPRVLTAEEQEMFRDFDYIADWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEA0</accession>
    <name>W5MEA0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Midkine</fullName>
            <shortName evidence="10">MK</shortName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Secreted protein that functions as cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642729.1">
        <property type="nucleotide sequence ID" value="XM_006642666.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015194088.1">
        <property type="nucleotide sequence ID" value="XM_015338602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEA0"/>
    <dbReference type="FunCoup" id="W5MEA0">
        <property type="interactions" value="943"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006709"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006717.1">
        <property type="protein sequence ID" value="ENSLOCP00000006709.1"/>
        <property type="gene ID" value="ENSLOCG00000005557.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692381"/>
    <dbReference type="KEGG" id="loc:102692381"/>
    <dbReference type="CTD" id="30277"/>
    <dbReference type="eggNOG" id="ENOG502S022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007640"/>
    <dbReference type="HOGENOM" id="CLU_136864_0_0_1"/>
    <dbReference type="InParanoid" id="W5MEA0"/>
    <dbReference type="OMA" id="WSECAEW"/>
    <dbReference type="OrthoDB" id="8818336at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005557">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.60.10:FF:000002">
        <property type="entry name" value="Midkine a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.60.10">
        <property type="entry name" value="Pleiotrophin/Midkine, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000762">
        <property type="entry name" value="Midkine_heparin-bd_GF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020091">
        <property type="entry name" value="PTN/MK_diS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020089">
        <property type="entry name" value="PTN/MK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037122">
        <property type="entry name" value="PTN/MK_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020092">
        <property type="entry name" value="PTN_MK_heparin-bd_GF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850:SF2">
        <property type="entry name" value="MIDKINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850">
        <property type="entry name" value="PLEIOTROPHIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05196">
        <property type="entry name" value="PTN_MK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00269">
        <property type="entry name" value="PTNMIDKINE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00193">
        <property type="entry name" value="PTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57288">
        <property type="entry name" value="Midkine"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00619">
        <property type="entry name" value="PTN_MK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5 10" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="9 10" id="KW-0497">Mitogen</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865968" description="Midkine" evidence="10">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine N-terminal" evidence="13">
        <location>
            <begin position="26"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="12">
        <location>
            <begin position="82"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="145" mass="16055" checksum="54612CA95A98FE52" modified="2014-04-16" version="1">MRGLFSMIIFLLVVLMMVTTQAGKEKKDKKKSKGTSDCAEWTWSPCVPNSGDCGVGTREGTCKEQTKKLKCKVPCNWKKEFGADCKYKFGNWGECDTATGVKNRSGTLKKALYNAECQQNIKVTKPCPTKTKSKAKGKKGRGKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEZ9</accession>
    <name>W5MEZ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Orthopedia homeobox a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626781.1">
        <property type="nucleotide sequence ID" value="XM_006626718.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEZ9"/>
    <dbReference type="FunCoup" id="W5MEZ9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006958"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006966.1">
        <property type="protein sequence ID" value="ENSLOCP00000006958.1"/>
        <property type="gene ID" value="ENSLOCG00000005761.1"/>
    </dbReference>
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    <dbReference type="CTD" id="560759"/>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159952"/>
    <dbReference type="HOGENOM" id="CLU_056068_0_0_1"/>
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    <dbReference type="OMA" id="MLPGEDI"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005761">
        <property type="expression patterns" value="Expressed in larva and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000124">
        <property type="entry name" value="Orthopedia homeobox b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051895">
        <property type="entry name" value="OTP_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="15">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="33"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="320" mass="34112" checksum="AB23F44628690D66" modified="2014-04-16" version="1">MLSHADLLDARLGMKDAAAELLGHREALKCRLGAGGSDPGHPGDLAPGSDTVEGTTLLPGDDINNTGSNPSGVQVSTKDQEKQQQQNPNQSGGQQSQQKQKRHRTRFTPAQLNELERSFAKTHYPDIFMREELALRIGLTESRVQVWFQNRRAKWKKRKKTTNVFRAPGTLLPTHGLPQFPSAAAAAAAMGDSLCSFHANDTRWAAAGMPGVSQLQLPPALGRQQAMAQSLSQCSLGAGPPPNSMSLSNSLSAASNGSGLQSHLYQPAFPGMVPASLSGPTNVTGSPQLCSSPDSDVWRGTSIASLRRKALEHTVSMSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFJ8</accession>
    <name>W5MFJ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Docking protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOK family. Type B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069037470.1">
        <property type="nucleotide sequence ID" value="XM_069181369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFJ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007157"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007166.1">
        <property type="protein sequence ID" value="ENSLOCP00000007157.1"/>
        <property type="gene ID" value="ENSLOCG00000005930.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696019"/>
    <dbReference type="KEGG" id="loc:102696019"/>
    <dbReference type="eggNOG" id="KOG4047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160143"/>
    <dbReference type="HOGENOM" id="CLU_057256_1_0_1"/>
    <dbReference type="InParanoid" id="W5MFJ8"/>
    <dbReference type="OMA" id="KMLSIEC"/>
    <dbReference type="OrthoDB" id="6279276at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005930">
        <property type="expression patterns" value="Expressed in heart and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14678">
        <property type="entry name" value="PH_DOK4_DOK5_DOK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13164">
        <property type="entry name" value="PTB_DOK4_DOK5_DOK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000110">
        <property type="entry name" value="Docking protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000082">
        <property type="entry name" value="Docking protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037816">
        <property type="entry name" value="DOK4/5/6_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF44">
        <property type="entry name" value="DOCKING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="3">
        <location>
            <begin position="132"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="37278" checksum="24A83BFB1D3BEE02" modified="2014-04-16" version="1">MASNFNDIVKQGYVRMRSRKLGIYRRCWLVFKKSSSKGPRRLEKYPDEKSAYIRACPKVTEISNVKNVTRLPRDTKRQAVAIVFTDDTSRTFTCESELEAEEWYKILSIECLGARLNDISLGEPDLLAAGVQCEHTERFNVFLLPCPNLDIYGECMMQITHENIYLWDIHNPRTKLVTWPLCSLRRYGRDATRFTFEAGRMCDSGEGLYTFQTREGEQIYQRVHSATLAIAEQHKRVLLEMEKSSRVLSKGSEPGSYPCTPTAILPRSAFWHHITGTQGGVDTGNGDMFSSSQTDSDSDLLPARLPSERRSATALSRHPSSFPTYPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGB2</accession>
    <name>W5MGB2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGB2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007421"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007429.1">
        <property type="protein sequence ID" value="ENSLOCP00000007421.1"/>
        <property type="gene ID" value="ENSLOCG00000006143.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263497"/>
    <dbReference type="HOGENOM" id="CLU_168377_3_1_1"/>
    <dbReference type="InParanoid" id="W5MGB2"/>
    <dbReference type="OMA" id="WMEADID"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006143">
        <property type="expression patterns" value="Expressed in brain"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="67" mass="7335" checksum="983576DD9EE16713" modified="2014-04-16" version="1">FCSSSVAQARRAVDQLRLEASVDRIKISIAATDLVRFCQDHSKSDPLLTGIPTSSNPFKDKKSCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGK4</accession>
    <name>W5MGK4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">3'-phosphoadenosine 5'-phosphosulfate synthase 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGK4"/>
    <dbReference type="FunCoup" id="W5MGK4">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007513"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007521.1">
        <property type="protein sequence ID" value="ENSLOCP00000007513.1"/>
        <property type="gene ID" value="ENSLOCG00000006215.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009613"/>
    <dbReference type="HOGENOM" id="CLU_009463_3_0_1"/>
    <dbReference type="InParanoid" id="W5MGK4"/>
    <dbReference type="OMA" id="IEIYKHH"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006215">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000212">
        <property type="entry name" value="Adenylyl-sulfate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000001">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF16">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="PUA domain-like"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="53"/>
            <end position="205"/>
        </location>
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            <begin position="235"/>
            <end position="388"/>
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            <begin position="397"/>
            <end position="619"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023268"/>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="626" mass="70673" checksum="76E047FC967C3759" modified="2014-04-16" version="1">YSLPVLLLKNKCLNLSKLAGNWDLQRSTNVVYQAHHVSRSKRGQVVGTRAGFRGCTVWLTGLSGAGKTTIGFALEEYLVSHAIPCYSLDGDNIRHGLNKNLGFTSTDREENIRRIAEVAKLFADAGLVCITSFISPYTKDRNEARKIHESAGLPFFEVFINAPLDVCESRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTGEESVNECIQQVVELLKEQSIVPSAILEDVNELFVPENKINLVRADANTLPTLEITKLDLQWVQVLAEGWATPLTGFMRESEFLQVLHFGTLLDGGTINMSVPIVLPVSTENKENLDGCAAFALVYEGRRVAILRNPEFYEHRKEERCARQWGTTCTQHPYIKMVMESGDWLVGGDLEVLERIKWNDGLDQFRLTPRELKHKFKEMKADAIFAFQLRNPVHNGHALLMQDTKRRLLDRGYKRPVLLLHPLGGWTKDDDVPLAWRMKQHAAVLEERVLDPNSTIVAIFPSPMLYAGPTEVQWHCRARMIAGANFYIVGRDPAGMPHPETRKDLYEPTHGGKVLTMAPGLTSVEIIPFRVAAYNKTKKAMDFYDPERHSEFEFISGTRMRKLARDGENPPDGFMAPKAWKVLTEYYSSQQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGK5</accession>
    <name>W5MGK5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nuclear receptor subfamily 1, group D, member 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="10">
        <location>
            <begin position="239"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
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            <begin position="165"/>
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        </location>
    </feature>
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        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
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            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007514.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="39886" checksum="38BF88116436881D" modified="2014-04-16" version="1">MRYWKRERERSERAIKALYTCIQSEGYSRPHIAFTVSARLGCPCPARRCAPGVPCRRPALPPGDPRLHGQQYSQAERESRAKLPTRRNMDNSPAVRFGRIPKREKQRLLDEMQSYMNSLNESASMEIDCSPPEAPPSPGESQSEEAIGAISRAYRDIFVSGQDRLGRRGTTQSSSSSNNNNHSPVFQNSPRPESSHPHAHHQGYPSYPTSFQTAAHRCPMAPRDPNPALPFVDNSRYGYQPSTSSNQSQCPSGVGPPPPTYPTNYNGYPLAETVGQSSCPWRIGGGAKVLACPLNACPVAPAGLSSQQVWESFSQCFTPAVREVVEFAKSIPGFQSLSQQDQVMLLKAGTFQVHSTSLPQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGT9</accession>
    <name>W5MGT9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:136864</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0390 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000006293.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="InParanoid" id="W5MGT9"/>
    <dbReference type="OMA" id="IEHETAM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006293">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16967">
        <property type="entry name" value="LEYDIG CELL TUMOR 10 KDA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16967:SF1">
        <property type="entry name" value="LEYDIG CELL TUMOR 10 KDA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="86" mass="9546" checksum="939969722000B587" modified="2014-04-16" version="1">MAQGKQKFKAQRPGVKKAQQQHKNKGPKKGARAIAPKKAQVVQQQKLKKSLEVAIRSHIEQEVTQRASTSLHKRLSLLKSTEGKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MII2</accession>
    <name>W5MII2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">carbonyl reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.1.1.184</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.184" evidence="4"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MII2"/>
    <dbReference type="FunCoup" id="W5MII2">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008191"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008201.1">
        <property type="protein sequence ID" value="ENSLOCP00000008191.1"/>
        <property type="gene ID" value="ENSLOCG00000006781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683960"/>
    <dbReference type="KEGG" id="loc:102683960"/>
    <dbReference type="CTD" id="873"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046499"/>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_1"/>
    <dbReference type="InParanoid" id="W5MII2"/>
    <dbReference type="OMA" id="YWANDSV"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006781">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963:SF4">
        <property type="entry name" value="CARBONYL REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963">
        <property type="entry name" value="CARBONYL REDUCTASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="276" mass="30160" checksum="F7662EA0893C5F0B" modified="2014-04-16" version="1">MSTPRVAVVTGSNKGIGFAVVRALCKQFTGDVYITARDVGRGQAAVQSLKSEGLNPLFHQLDITDLTSIRTLRDFLKERYGGIDVLINNAGIAFKVQDTTPFGIQAEVTLRTNFFATRDICNEILPIVKPGGRVVNVSSIAGSMALKQCSPELQARFRSDDITEEELVRLMERFVEEAKSGEHTQRGWPNWGYGVSKTGLTVLSRIQARMLQKERPQDGILLNACCPGWVRTDMAGPKATKSPDEGAVTPVYLALLPPGADGPHGEFVSEKQVQAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJX6</accession>
    <name>W5MJX6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sterile alpha motif domain containing 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJX6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008685"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008695.1">
        <property type="protein sequence ID" value="ENSLOCP00000008685.1"/>
        <property type="gene ID" value="ENSLOCG00000007177.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693446"/>
    <dbReference type="CTD" id="344658"/>
    <dbReference type="eggNOG" id="KOG3829">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160075"/>
    <dbReference type="HOGENOM" id="CLU_046957_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJX6"/>
    <dbReference type="OMA" id="MLYMMNL"/>
    <dbReference type="OrthoDB" id="9943471at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007177">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09579">
        <property type="entry name" value="SAM_Samd7_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF89">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="394"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="525" mass="57952" checksum="00A231B60227F7D2" modified="2014-04-16" version="1">MTPRDQLRKMSALGEQGPLDEKHWYRLVNGMSAGAELRQRQELIIRNQMAMNPQLLAPGQHRLQGLSAPFEPRLMERELVPPSEMVPSEARQIHMSAHLGPPIPQHSSVLPGRPYPGAGYGFLPTEPIESVARRQEMIHKQNLARMEMNAILHQKELESAHQKGMMGMEAPLMYTGIQANAMAFRSRQRLPEGHLPSDVYVHRTTLEDLQGNALLMSSSPYPPISTLHRERGRRPGRRATNHKSAEGNALGPKGQSEEKVVEQSPGGSGEEKEVDCKGENGNENANKMDQSKMDVELSSGSGKNHKEYEQVLRKNCSSHDSCTESSNCNTACSTSDKDLPNPCSLDKYVYPSANPLSNLPYGFPVPGNGLLPPGSHGVFLNGEEMSSIEDIRKWTVEDVYNFINNIPGCSEYAQTFKDHIIDGETLPLLTEDHLLDTLGLKLGPALKVRSQVSRRLGSMFYMMNLPLAATAMQSVPEKGTGDQSSDINSPLTCNTVELLSSPCPRETESTKPPEQSLAQENTCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJY8</accession>
    <name>W5MJY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Akt interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641474.1">
        <property type="nucleotide sequence ID" value="XM_006641411.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015224135.1">
        <property type="nucleotide sequence ID" value="XM_015368649.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJY8"/>
    <dbReference type="FunCoup" id="W5MJY8">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008697"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008707.1">
        <property type="protein sequence ID" value="ENSLOCP00000008697.1"/>
        <property type="gene ID" value="ENSLOCG00000007183.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688939"/>
    <dbReference type="KEGG" id="loc:102688939"/>
    <dbReference type="CTD" id="64400"/>
    <dbReference type="eggNOG" id="KOG0429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010125"/>
    <dbReference type="HOGENOM" id="CLU_083049_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJY8"/>
    <dbReference type="OMA" id="WGFPEWR"/>
    <dbReference type="OrthoDB" id="5596422at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007183">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:DNA damage tolerance"/>
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        <property type="term" value="P:protein K63-linked ubiquitination"/>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="UEV_AKTIP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AKT-interacting protein-like isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
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    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
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        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBC_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="75"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="291" mass="33146" checksum="724E2C176C5FB80C" modified="2014-04-16" version="1">MNPFWSMSASTGRKRAEGEEKSGAVEQRVSPTRAPYGKKQLPSIPKNAVPITKPVSPAASSQASNGTHASYGPFYLEYSLLAEFTLVIKQKLPGIYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRVVFDIPVFNPLVDPVSGELDVKRAFTKWRRNHNHIWQVLMYARTVFYKIQTLEPLNPEAAVLYEKDIQLFKSKVVESVKLCNSHLFDQPKIDDPFAISFSPWNPSVHEEVRGRMFMHKRRPEDHHKGLQVSGLSWVKPGSTQPFSKDDNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK48</accession>
    <name>W5MK48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).</text>
    </comment>
    <comment type="function">
        <text evidence="11">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
            <location evidence="2 11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the ARPC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006640420.3"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000008767.1">
        <property type="protein sequence ID" value="ENSLOCP00000008757.1"/>
        <property type="gene ID" value="ENSLOCG00000007235.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="loc:102694512"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018018"/>
    <dbReference type="HOGENOM" id="CLU_094365_1_0_1"/>
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    <dbReference type="OrthoDB" id="200404at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007235">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARPC3"/>
    </dbReference>
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        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARP2/3_P21-Arc"/>
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    </dbReference>
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        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="178" mass="20553" checksum="A18591426C3F77FB" modified="2014-04-16" version="1">MPAYHSNLMDPDTKLIGNMALLPLRTQFKGPAPRETKDSDIIDEAIYYFKANVFFKNYEIKNEADRTLIYVTLYISECLKKLQKCNSRGQGEKEMYTLGITSFPIPGEPGFPLNAMYAKPADKQEEETMRAYLQQIRQETGLRLCEKVFDPQTDKPSKWWTCFVKKQFMNKSLSAPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML27</accession>
    <name>W5ML27_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dispatched RND transporter family member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009097.1">
        <property type="protein sequence ID" value="ENSLOCP00000009086.1"/>
        <property type="gene ID" value="ENSLOCG00000007482.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3664">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157931"/>
    <dbReference type="HOGENOM" id="CLU_007038_0_0_1"/>
    <dbReference type="InParanoid" id="W5ML27"/>
    <dbReference type="OMA" id="VARWKMH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007482">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042480">
        <property type="entry name" value="DISP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053954">
        <property type="entry name" value="DUF7023"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687:SF1">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22894">
        <property type="entry name" value="DUF7023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="971"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1057"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1118"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="3">
        <location>
            <begin position="339"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1151" mass="124929" checksum="EB50F2EDE37938CD" modified="2014-04-16" version="1">MDSEDDPSMLQSDWPEEEEDEEDEGVEGEEEEGGSPGTVPPRAAGSSRWVWGAVGWVYTRPWACCLVLALGVLLPCALSAFMFLRCPPLDIDLSYSAFEVRGHTSSERFDALTLALKAELGSWGRSRRDVDGYTAQALRDLLLQHLQRQRGGAAEGGGMSGGGGAAEGGSSGASGASETELDPELDPELDPAQAKQREAPARRGRLWPPIAPRETDRARRREMGVPREAYPHGEFVIPSYTQSHAHWRMELVFVAQGEGDPNIFTPERLRTIHAVERRVMEHPQFRQFCWKPPEVLRDPPLGPFSYCSPPXPTVFLSFFGLASIGLSCLVALFLYHVGFGVRYLGILNGVAAFVIIGIGVDDVFVFINTFQQAGHLRQPGQRMVHTVQTAGRATFLTSFTTAAAYAANTFSQIPAVHDFGLFMALVVSCCWLWVLLMLPAALCVWSRWLSPRETACLRWYERRPPRPSSNLCTAPREPQGRSCNSSELASLNQFICLFAKHLMLFCNSCNSNLVHFLRINTARRLHSIQLQQVRQLPLVEKHDCASLPRCAQHLFIRAEEIQIFDSMKFSNLFSKMLILVTCKFPHPSRLTSPQVSAALGSSAGDQGTFWKSVSPPAGLLLTVYVSKLQAGAGPPLYRFSLNASVPLPWKALTPGNGEVPSFQAFGGPHRNFTTRLTVCVAAAGQPRPAGMITSRSCDPSRGWRSEFSFYVASAEQQHSRKLYFAQLRRSPFHSRVCAAPPGCVLSSGPDGPTKGSFYTPVNRDQQGAVGSATSGFNPCENGACGRPAVRPLVDTGAMVFVVFGILGVNRTRHTGNHVIGDMGSVIYDPTFDLFREIGHLCRLCKTIGSNSHLVKPGGAQCLPSGSSLSSLLPLLHPECQSLPQPSLLPGQLSHGALGLQDGAVRWVSMAFESTTYKGKSSFQTLSDFLQWESFLQEQLSALPQSSALRRGFQTCEHWKQIFMEILGVQSALYSLLLSLAICIAAVSVFTAHLRLLLPVLLTILGENAPEPGNAFTNGFSSSAHEIKILLDSLSLQRLPLSLQRLPLSLYSVSLSPALSAVTTVIATVPLFFCVIAPFAKFGKIVALNTAVSVAFTLSVGAALLATMGPARFSRPPAAVLQAGLAVLGLGAAGVALRWALGYLGVPAAWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML71</accession>
    <name>W5ML71_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BCL2 interacting protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630463.1">
        <property type="nucleotide sequence ID" value="XM_006630400.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML71"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009130"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009141.1">
        <property type="protein sequence ID" value="ENSLOCP00000009130.1"/>
        <property type="gene ID" value="ENSLOCG00000007528.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688967"/>
    <dbReference type="KEGG" id="loc:102688967"/>
    <dbReference type="CTD" id="664"/>
    <dbReference type="eggNOG" id="ENOG502QQ4B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013415"/>
    <dbReference type="HOGENOM" id="CLU_091463_1_0_1"/>
    <dbReference type="InParanoid" id="W5ML71"/>
    <dbReference type="OMA" id="MYNGDME"/>
    <dbReference type="OrthoDB" id="5857140at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007528">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043068">
        <property type="term" value="P:positive regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF4">
        <property type="entry name" value="BCL2_ADENOVIRUS E1B 19 KDA PROTEIN-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="198" mass="22215" checksum="2FA0313F99DCC8F9" modified="2014-04-16" version="1">MSTEKQSNQDENLQGSWVELHFNNNGNGSVTQNGGQDQVPASIHNGDIENMLLDAQHESGRSSSRGSSHCDSPPRSQTPLHLRRGSEVHSSGEKNSSQSEEDYLERRREVENIMKKNADWIWDWSSRPENAPPKEFLFKHPKRSATLSMRNTSVMKKGGIFSAEFLKVFLPSLFLSHILAVGLGIYIGRRLTTSTSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLP1</accession>
    <name>W5MLP1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-type tyrosine-protein phosphatase</fullName>
            <ecNumber evidence="17">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer. Isoform 2: Homodimer. Can form oligomers. Dimerization is increased by oxidative stress and decreased by EGFR. Isoform 2 interacts with GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01004038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004039">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202503.1">
        <property type="nucleotide sequence ID" value="XM_015347017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009300"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009311.1">
        <property type="protein sequence ID" value="ENSLOCP00000009300.1"/>
        <property type="gene ID" value="ENSLOCG00000007653.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690063"/>
    <dbReference type="CTD" id="100499203"/>
    <dbReference type="eggNOG" id="KOG4228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156570"/>
    <dbReference type="HOGENOM" id="CLU_001645_8_0_1"/>
    <dbReference type="InParanoid" id="W5MLP1"/>
    <dbReference type="OMA" id="FCVHSLQ"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007653">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000007">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000022">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016336">
        <property type="entry name" value="Tyr_Pase_rcpt_a/e-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF499">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002006">
        <property type="entry name" value="PTPR_alpha_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 17" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="123"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="302"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="414"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="588"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="18">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="18">
        <location>
            <position position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="323"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine" evidence="20">
        <location>
            <position position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR002006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR002006-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR002006-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR002006-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="683" mass="78598" checksum="EC43658A79B555F6" modified="2014-04-16" version="1">MMVPVLLAWGIYTMTSPTAPSNTTEPATGMGKDYILPSVLVIFLLLMIIVLLAVYFLRFRNQGKAVVTTVDKKIPNGILEEQEQQTVVLLPRSPSVSKKYLPIPVDSLEEEYRIRSADDGKFFREEFNSLPSGNSQGSCEEANKEDNKEKNRYPNILPYDHSRVVLSQVDGNSCSDYINASYIDGYKEKNKFIAAQGPKQETVADFWRMIWEQKSATIVMLTNLKERKEDKCYQYWPDQGCWTYGNIRVSVEDFTVLVDYTIRKFCVQYQSSDGSKAPRLVTQLHFTSWPDFGVPFTPIGMLKFLKKVKTVNPSFVGPIVVHCSAGVGRTGTFIVIDAMIDMMHAEQRVDVFGFVSRIREQRSQLVQTDMQYSFIYQALLEYYLYGDTELDVSSLEGHLQKLHNTTAPSDRIGLEEEFKKLTNVRIMKENMRTGNLPANMKKNRVLQIIPYDFNRVILSMKRGQEFTDYINASFIDGYRQKDYFIATQGPIAHTVDDFWRMVWEWKSHSIVMLTELQEREQDKCFQYWPPEGSVTYSDYTVEIKGETLVDTFSVRDFVLTYKPDNQTRLVRHFHFHGWPEIGIPAEGKGMIDIIAAVQKQQQQSGNHPIIVHCSAGAGRTGTFIALSNILERVKAEGLLDVFQTVKSLRMQRPHMVQTMEQYDFCYRVVQDFVDIFSDYANFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLS8</accession>
    <name>W5MLS8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01007653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLS8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009337"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009348.1">
        <property type="protein sequence ID" value="ENSLOCP00000009337.1"/>
        <property type="gene ID" value="ENSLOCG00000007686.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00470000042444"/>
    <dbReference type="HOGENOM" id="CLU_1724338_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLS8"/>
    <dbReference type="OMA" id="ENKICAS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007686">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053116">
        <property type="entry name" value="GATA-type_Znf_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341:SF1">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="160" mass="18371" checksum="7533471FC952B4E3" modified="2014-04-16" version="1">RAPRKQQNPTRSAARYDPNFRGVTMHMEMQLNDRRADQCQLVITPHYRYCAPKSKGSLFVTAGVKKTHPISNLVSAHLNPTMLLAENKICASCDTRKTPLWRDAEDGTPLCNACGIRYKKYRIRCFHCWHIPKKEGNSNSRCFRCGNALRVAAYRKNSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMC4</accession>
    <name>W5MMC4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Macrophage-expressed gene 1 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Perforin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Pore-forming protein that plays a central role in antigen cross-presentation in dendritic cells by mediating delivery of antigens for cross-presentation. Dendritic cells bridge innate and adaptive immunity by capturing exogenous antigens on MHC class-I molecules and presenting them to naive CD8(+) T-cells. Acts by forming a pore in antigen-containing compartments, promoting the release of antigens into the cytosol, enabling generation of MHCI:peptide complexes and T-cell priming.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Phagosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MPEG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMC4"/>
    <dbReference type="FunCoup" id="W5MMC4">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009533"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009544.1">
        <property type="protein sequence ID" value="ENSLOCP00000009533.1"/>
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        <property type="component" value="Linkage group LG9"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
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        <property type="term" value="C:phagocytic vesicle membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:innate immune response"/>
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    <dbReference type="CDD" id="cd22579">
        <property type="entry name" value="MPEG1_P2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MACPF"/>
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    <dbReference type="InterPro" id="IPR039707">
        <property type="entry name" value="MPEG1"/>
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        <property type="entry name" value="MACROPHAGE-EXPRESSED GENE 1 PROTEIN"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866324" description="Macrophage-expressed gene 1 protein" evidence="21">
        <location>
            <begin position="27"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="22">
        <location>
            <begin position="36"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="687"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004265"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007256"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021365"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023130"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00045657"/>
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            <dbReference type="ARBA" id="ARBA00045689"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
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            <dbReference type="PROSITE" id="PS51412"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009533.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="727" mass="78354" checksum="53D95648F0CAE7A1" modified="2014-04-16" version="1">MAPTFRAWLQSALTLLAMTFPRSCSSLPVSQRSGSGIQECRKTLAMPVLEVLPGGGWDNLRNLDMGRVMNVSYALCQTTEDGAYLIPDEVFVVPQKVSNVETHSELIESWLQQSSSVARSINADVSFLGVLNGKFSAESQRMKTHQVRESSVTTRVQVRNFMYSVKAHPGFSFDSRFRQQAGEIAEALENNQTRRAAFLAEMLVLQYGTHVLTAVDAGASLVQEDYLRSSFLSSSQSEGFSFSAAVGLNFFNKVNVGLHGGGKDTDSLTKAYQGNVTYSVTQSHGGAPFYPGITLQKWQESTANNLVAIDRSGLPLHSVLNPATLPDLPAPSVRKLARAVRDAVRRYYTVNTHPGCVKPDSPNFNFQANVDDDSCEGPATNLSFGGVYQRCAPLSPDAGPLCRVLEQKNPQTGSLGCQQPYSPTLLRSEVREESYSRLECRRECQRCWLILSCCEQVCGDAYRVRRAQIDTYWCATEGPAPPLSGFLFGGLYGPSQQNPFTNSQACPPGFLALTLLSDGLKICQSADYEVGARFAVDFGGLFSCEAGNPLAAGRPRCPAGFSQHVAAVSDGCQVLYCVRSGVFNGGELLPVRLPPFTRPPLAGLGATNTVAVMMEGERSWIRDGQSRLWKVASPGQLQKMAEVFDGSPRGLSGGETFGVVLGAAVALALAAGAVAYGLKRRRRWGRGSQRGYDQIPSEQAHGAAGPDEVAETEMEGGGSGSTQPLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMI9</accession>
    <name>W5MMI9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxiredoxin-1</fullName>
            <ecNumber evidence="14">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer. Interacts with TIPIN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01007630">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631553.1">
        <property type="nucleotide sequence ID" value="XM_006631490.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMI9"/>
    <dbReference type="FunCoup" id="W5MMI9">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009598"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009609.1">
        <property type="protein sequence ID" value="ENSLOCP00000009598.1"/>
        <property type="gene ID" value="ENSLOCG00000007904.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682507"/>
    <dbReference type="KEGG" id="loc:102682507"/>
    <dbReference type="CTD" id="7001"/>
    <dbReference type="eggNOG" id="KOG0852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154277"/>
    <dbReference type="HOGENOM" id="CLU_042529_21_1_1"/>
    <dbReference type="InParanoid" id="W5MMI9"/>
    <dbReference type="OMA" id="HYAFGDV"/>
    <dbReference type="OrthoDB" id="185659at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007904">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045321">
        <property type="term" value="P:leukocyte activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF161">
        <property type="entry name" value="PEROXIREDOXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 14" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="6"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="15">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="198" mass="22034" checksum="897314D68215FDF3" modified="2014-04-16" version="1">MSSGNAKIGKPAPHFQATAVVDGQFKEIKLSDYRGKYVVFFFYPLDFTFVCPTEIVAFSDRAEEFRKINCEVIAASTDSQFSHLAWTNTPRKVGGLGAMKIPLVADLTQSISQDYGVLKEDEGIAYRGLFVIDDKGILRQITINDLPVGRSVDETLRLVQAFQFTDKHGEVCPAGWKPGSDTIVPDVQKSKEYFAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNB1</accession>
    <name>W5MNB1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Tartrate-resistant acid phosphatase type 5</fullName>
            <ecNumber evidence="2 6">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="7">Binds 2 iron ions per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="2 6"/>
    <dbReference type="EMBL" id="AHAT01016122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNB1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009870"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009882.1">
        <property type="protein sequence ID" value="ENSLOCP00000009870.1"/>
        <property type="gene ID" value="ENSLOCG00000008124.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016735"/>
    <dbReference type="HOGENOM" id="CLU_043332_1_0_1"/>
    <dbReference type="InParanoid" id="W5MNB1"/>
    <dbReference type="OMA" id="VWSIGNH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008124">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045453">
        <property type="term" value="P:bone resorption"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009612">
        <property type="term" value="P:response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07378">
        <property type="entry name" value="MPP_ACP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000062">
        <property type="entry name" value="Tartrate-resistant acid phosphatase type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024927">
        <property type="entry name" value="Acid_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161:SF29">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000898">
        <property type="entry name" value="Acid_Ptase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866288" description="Tartrate-resistant acid phosphatase type 5" evidence="10">
        <location>
            <begin position="22"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="9">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="162"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000898-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="325" mass="36734" checksum="31093BEAF229EB23" modified="2014-04-16" version="1">MGKVTCLLLVCLQAILSNCQSKENDYLRFVAIGDWGGSPDSPFFTPLEKAVAAEMGRVAQTVGLDFVLSLGDHFYYQGVKDIDDFRFKATFENVFADPSLLSVPWYLVAGNHDHLRNVTAQVAYSKRSKRWNFPELYYDLYFKVPHSNTSVTILLIDTVLLCGNTYDGIQPEKPENHEAAAKQFIWIKEKLEKSKSEFLIVAGHYPVWSIGHHGPTQCLVEQLRPLLKKHNVTAYLSGHDHSLQFIQEDNGMAYIVSGSGNFVDMSIYHKNKFPHAWQLFSSAVNNTFGGFAYFTVTKEKMFINYISAAGEGVYQTSLSKRSAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNE4</accession>
    <name>W5MNE4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ELOF1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Factor involved in transcription-coupled nucleotide excision repair (TC-NER), a mechanism that rapidly removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Acts as a key adapter required to anchor TC-NER factors to RNA polymerase II: stably positions UVSSA and the DCX(ERCC8) complex (also named CSA complex) on arrested RNA polymerase II, leading to neddylation and activation of the DCX(ERCC8) complex and ubiquitination of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631557.1">
        <property type="nucleotide sequence ID" value="XM_006631494.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204611.1">
        <property type="nucleotide sequence ID" value="XM_015349125.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204612.1">
        <property type="nucleotide sequence ID" value="XM_015349126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNE4"/>
    <dbReference type="SMR" id="W5MNE4"/>
    <dbReference type="FunCoup" id="W5MNE4">
        <property type="interactions" value="1627"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009903"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009915.1">
        <property type="protein sequence ID" value="ENSLOCP00000009903.1"/>
        <property type="gene ID" value="ENSLOCG00000008159.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683313"/>
    <dbReference type="KEGG" id="loc:102683313"/>
    <dbReference type="CTD" id="84337"/>
    <dbReference type="eggNOG" id="KOG3214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000053"/>
    <dbReference type="HOGENOM" id="CLU_105983_2_0_1"/>
    <dbReference type="InParanoid" id="W5MNE4"/>
    <dbReference type="OMA" id="CLDANKK"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008159">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000002">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="83" mass="9505" checksum="6212F984216F83FE" modified="2014-04-16" version="1">MGRRKSKRKPPPKKKMTGNLDTQFTCPFCNHEKSCDVKMERSRNTGIISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNI3</accession>
    <name>W5MNI3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">H6 family homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630481.1">
        <property type="nucleotide sequence ID" value="XM_006630418.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNI3"/>
    <dbReference type="FunCoup" id="W5MNI3">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009942"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009954.1">
        <property type="protein sequence ID" value="ENSLOCP00000009942.1"/>
        <property type="gene ID" value="ENSLOCG00000008191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692340"/>
    <dbReference type="KEGG" id="loc:102692340"/>
    <dbReference type="CTD" id="3167"/>
    <dbReference type="eggNOG" id="KOG0485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160392"/>
    <dbReference type="HOGENOM" id="CLU_064096_1_0_1"/>
    <dbReference type="InParanoid" id="W5MNI3"/>
    <dbReference type="OMA" id="CTQHQAH"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008191">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048916">
        <property type="term" value="P:posterior lateral line development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="272" mass="30269" checksum="949C45D8598EA081" modified="2014-04-16" version="1">MNSKEDNGSKCSPAPISSFTIQSILGTTSEGGRSGTKENSKGGLQRKRTLSVSSEEDCSAGEDSADCFCSEPGHSETCNHQHQPLNFTCLNAAKGITPGQEAIERRQILTQPLLQDYKEDKETPCSQISPISEERQRDGADKQSSSAKKKTRTVFSRSQVYQLESTFDMKRYLSSSERACLASSLQLTETQVKTWFQNRRNKWKRQLSAELEAANMAHASAQTLVGMPLVFRDNSLLRVPVPRSIAFPTPLYYPGSNLPALPLYNLYNKIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNI8</accession>
    <name>W5MNI8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01036467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNI8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009947"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009959.1">
        <property type="protein sequence ID" value="ENSLOCP00000009947.1"/>
        <property type="gene ID" value="ENSLOCG00000008194.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154558"/>
    <dbReference type="HOGENOM" id="CLU_075585_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNI8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008194">
        <property type="expression patterns" value="Expressed in bone element and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="153"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="282" mass="32457" checksum="B64BEDC3EE2B7AF8" modified="2014-04-16" version="1">MADDNLYVNVKTSRVRSQQCRALRSPSAQQEAQHTLSRCRSCVLMALGVTVCVLLATVIALGTVLWKNSLKLSNAELERIKNELERCKSKVKENQQLKNQGQVEEFMIEQEMKRQQSAHLEERKKEIDPVFCVEPYTGERKQQCCPEGWKGGDSGRCYYVSTDRRPWESASQFCSSVGAQLVVTEDKRELEMLRDLVQEHYYWMGLSRRDSSAVWTWSGGRELNNEMVTLKEGSWDGDCAYGHWSQARITLYSRKCEGHLHWICEQRCVSMPVCLSSGNKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNM9</accession>
    <name>W5MNM9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 7 member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNX3</accession>
    <name>W5MNX3_LEPOC</name>
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        </submittedName>
    </protein>
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        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="2 7">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR028739"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <sequence length="491" mass="54794" checksum="DEBE48C540BC0733" modified="2014-04-16" version="1">MNSWWKDWSSSWVYHTVVLSVFGFFANFRPAEPFLTPYLVGPYKNISEEQVTNILYPIWTYSYLALLLPVFLLTDYLRYKPIIVVQGVCLVINWTLLCFAQGMPAMKYLHFNNGIATATDVAYFSYIYCVIPSDRYQKVTSYLRTATLVGYTVGSTLGQLLVSVAGLPYFYLNAINLGSALMACLSSFLLPMPRAGGMFFNKETAAPPGEQCQDSRSHDREEDRPSTPPGDIKPPRKKGQAAGKLRRKNLGCAVHLLWTSFIDCYSSRKLIYWSLWWAFATCGYYQIFNYIQLMWNHIEPSGSSPVFNGGVEAVTTLVGAIAAFSVGFMKLDWDIWGELALGLFSAISSGAVYATVLTDSIWVSYAGYVTFKASYMLLITITTFQIAANLSLECYALVFGINTFVALLLQTILTAIVVDESGLGLSIVKQFIVYGTYYVVISLLFLGRGVYTMSRYKCRSRNDSSQDVPGDSVVISSCDEEEACLIKSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP83</accession>
    <name>W5MP83_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anti-silencing function 1A histone chaperone</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211198.1">
        <property type="nucleotide sequence ID" value="XM_015355712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP83"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010192"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010204.1">
        <property type="protein sequence ID" value="ENSLOCP00000010192.1"/>
        <property type="gene ID" value="ENSLOCG00000008391.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689123"/>
    <dbReference type="eggNOG" id="KOG3265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004692"/>
    <dbReference type="HOGENOM" id="CLU_060354_1_2_1"/>
    <dbReference type="InParanoid" id="W5MP83"/>
    <dbReference type="OMA" id="CALYKQQ"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008391">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF8">
        <property type="entry name" value="HISTONE CHAPERONE ASF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="22142" checksum="C2409646E35F5804" modified="2014-04-16" version="1">MYNLMSPVGTEKRRYSFEITFECIEDLPEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAANPGLIPDADAVGVTVVLITCTYRAQEFIRVGYYVNNEYTDPELRENPPVKPDFTKLQRNILASNPRVTRFHINWDDSSEKMDDSENTNPNMQPILPSNSIPSASKGLAVTGNSLHLMSDSLMNCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPY4</accession>
    <name>W5MPY4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.15</ecNumber>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a fatty acid(in) = a fatty acid(out)</text>
            <dbReference type="Rhea" id="RHEA:38879"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="13"/>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01007565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPY4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010443"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010457.1">
        <property type="protein sequence ID" value="ENSLOCP00000010443.1"/>
        <property type="gene ID" value="ENSLOCG00000008584.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697361"/>
    <dbReference type="KEGG" id="loc:102697361"/>
    <dbReference type="CTD" id="541410"/>
    <dbReference type="eggNOG" id="KOG1179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159323"/>
    <dbReference type="HOGENOM" id="CLU_000022_46_2_1"/>
    <dbReference type="InParanoid" id="W5MPY4"/>
    <dbReference type="OMA" id="HHGLIMR"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008584">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090434">
        <property type="term" value="F:oleoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001676">
        <property type="term" value="P:long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001579">
        <property type="term" value="P:medium-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000008">
        <property type="entry name" value="Long-chain fatty acid transport protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF7">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="23">
        <location>
            <begin position="82"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023098"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00026113"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
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            <dbReference type="ARBA" id="ARBA00041297"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="647" mass="71527" checksum="702BF3579AD56D71" modified="2014-04-16" version="1">MRTLAGATLSLGSLGLLRLFGVPWSWSLAASLGVYLGTGGWHYFYIVLRTAKRDLKGLYVLLRVKLALRRHLRAGSTIPSIFSETARAHPDEPALIYEATGEVWTFAQLEHRSNAVGQWALAWGLAPGEVAAIFMESHPLMVALWLGLAKVGVEAALINFNLRRDALLHCVSVCGARVFIFGAELADAVEEVSDSLSKAMVRFCTGDISPGRLAALGAEPLDVLLASASHVHPPRTHHKGFNDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGYHAFGMRPDDIVYDCLPLYHSAGNIMGVGQCLVNGLTVVIKKKFSASRFWDDCVKYNCTVVQYIGEICRYLLSQPSREVERQHRVRLAVGNGLRPSVWEAFTQRFNIKQVGEFYGATECNCSIANMDGKVGACGFNSRILPNVYPIRLVKVNEETMELIRDRSGLCVSCRPGEPGLLVGRINQQDPLRRFDGYANESANTKKIAYNVFKKGDSAYLSGDVLEMDELGYMYFKDRSGDTFRWRGENVSTTEVEGTLSTILGQTDVAVYGVPVPGVEGKAGMAAIADGDGRFDQERFYREILKVLPPYARPVFLRISPQVDTTGTFKIQKTRLQKEGFDPRHSSDRIFFLAARAGGYKTLDEELYSAIIGGKVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ29</accession>
    <name>W5MQ29_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Adhesion G protein-coupled receptor E5b, duplicate 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ29"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010503.1">
        <property type="protein sequence ID" value="ENSLOCP00000010488.1"/>
        <property type="gene ID" value="ENSLOCG00000008623.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165798"/>
    <dbReference type="HOGENOM" id="CLU_002753_3_7_1"/>
    <dbReference type="InParanoid" id="W5MQ29"/>
    <dbReference type="OMA" id="DKKICRD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008623">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Fibrillin 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="GAIN_B"/>
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    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
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    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
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    <dbReference type="InterPro" id="IPR003056">
        <property type="entry name" value="GPCR_2_ADGRE2_ADGRE5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF433">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01278">
        <property type="entry name" value="CD97PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866472" evidence="16">
        <location>
            <begin position="25"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="670"/>
            <end position="688"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="700"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="40"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="91"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
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        <location>
            <begin position="314"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="19">
        <location>
            <begin position="483"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="742"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="762"/>
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        </location>
    </feature>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="773" mass="85872" checksum="16F563464DFAE766" modified="2014-04-16" version="1">IVTVWFFFFLGLYLCLTLILGVMSSCPSGFTNLSNGTCTEINECRENKTICGPNATCSNTIGSYNCTCNKGYKASPGEDKFNTNLDVTCEDINECQKNKTICGPNAKCSNTIGSYNCTCNKGYKNNRMTRRYVDECTQGLICGSNANCSNSAGSYWCVCHPGFSQSENSTGNCSDIDECKMIPPVCGPQTNCNNTPGSYSCIPFCGPGLINYGNGSSQCKKLNCNSLFKNEAGQEKLPSGLKKVVNDLNDSCLMISDQSYPDGNTYLMRFLGIVDDLLLGGVLDRKSVSSFLHTTEQAMMLIAPLMKESQTWINVSTLGVDFLVSRGSDPPTGSISLSSEYAVLDTHWETVASQDNQGFAAAALVTYYDLESSVNSSFDAVFRETGEKDRTSFQINSKVVTVSVSNEHTEKLEKSVHITFSNLKILQQKAEYKTCVYWNKGEWSTQGCVAKHFNDTHTVCECDHLSSFAVLMSLYDLKDSFELQMITWVGLSLSLLCLLLCIVTFYWCRSIQGTRNTIHLHLCISLFIADLVFLAGISRTQNKGGCALVAGLLHFFFLASFCWMCLEGIQLYRMVVLVFHTTLHRLHMLAFGYGIPLVIVVISAISYPGGYGTDRHCWLALDRGLIWSFFGPVCVIIMINAFFFMITVWKLALKFSSLNPDLSNLKKIKKFTITAVAQLCILGSMWIFGCFQFEENTIVMSYLFTILNSIQGVLIFVMHCLLSKPVRDEYRKFLPPVCRTEKKPKYSEFSSSTNNSANSQSLKNSRNTGESQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRH8</accession>
    <name>W5MRH8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">m7GpppN-mRNA hydrolase</fullName>
            <ecNumber evidence="13">3.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">mRNA-decapping enzyme 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01002868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222375.1">
        <property type="nucleotide sequence ID" value="XM_015366889.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRH8"/>
    <dbReference type="FunCoup" id="W5MRH8">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010987"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011003.1">
        <property type="protein sequence ID" value="ENSLOCP00000010987.1"/>
        <property type="gene ID" value="ENSLOCG00000009013.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683093"/>
    <dbReference type="CTD" id="167227"/>
    <dbReference type="eggNOG" id="KOG2937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018878"/>
    <dbReference type="InParanoid" id="W5MRH8"/>
    <dbReference type="OMA" id="YICKEDY"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009013">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1050:FF:000001">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="19">
        <location>
            <begin position="95"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="242"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="50762" checksum="D8C561A3C4A4FEAA" modified="2014-04-16" version="1">METKRGEIPSGVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIREFAKAVFNHCPFLLPQGEDVQKVLEQWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNEDEAPHDCAAREVLEETGFDIRDRICKDTFIEQRITDQLARLYIIPGVPKETKFNPKTRKEIRNIEWFSIEKLPSHKNDMTPKSKLGLAPNKFFMAIPFMRPLREWIAKKQGESSDSDNGFPPNGSTPAKPQMDKVRSRSRQNQTIVDGSSGDSWLKQKQQKPFSQSTQHDLSEFLKVKNLRGNGKKHQDSPNLKKRANGVSNQQVKHHQSAKAEKRLQPRRLQDNFETDAVCDFCSSNDEQPLTHAEGVPTTCNGECENVLYSSIGFCSSKQWTASSFNTVFCYVQVKSWHDLTKQLGHYSPRYHVLQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSD3</accession>
    <name>W5MSD3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heteroous nuclear ribonucleoprotein H3</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSD3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011292"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011308.1">
        <property type="protein sequence ID" value="ENSLOCP00000011292.1"/>
        <property type="gene ID" value="ENSLOCG00000009247.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157720"/>
    <dbReference type="InParanoid" id="W5MSD3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009247">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12729">
        <property type="entry name" value="RRM1_hnRNPH_hnRNPH2_hnRNPF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ESRP"/>
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        <property type="entry name" value="hnRNPH3_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="8"/>
            <end position="87"/>
        </location>
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    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="108"/>
            <end position="185"/>
        </location>
    </feature>
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            <begin position="293"/>
            <end position="383"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="431" mass="46982" checksum="8B493A9A4179286D" modified="2014-04-16" version="1">MSLNEEGFVVRIRGLPWSCTQEEVAGFFSDCNIVGGVNGVCFTYSKEGRPSGEAFVELKTAEDFKNAIAKDRKYMGHRYIEVFKSNRSEMDWVLKRCGPTDYDNCSGCMVRLRGLPFGCSKEEIVQFFAGLKIVPNGITLPMDYQGRSTGEAFVQFASKEIAEKALGKHKERIGHRYIEIFKSSRNEIRAYYELPRRMLAQRPGPYDRPMMGRGGFFGPGPGRGGSGVLDQMRSGGGGYSGGYGGFDNYNGFNNYCFGNGMFEDRIRGDRGRGMGGHGYSGAGDASSGFHSGHFVHMRGLPFRATEADIANKFFSPLNPVRVHIDVGPNGKSTGEADVEFGSHEDAVAAMSKDKNHMQHRYIELFLNSTASGGSEMECIISKSGKGKTEGNNGGYGSAGRMGISGGYESGYANPDNMGGYGKHHISNITDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT37</accession>
    <name>W5MT37_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain adjacent to zinc finger domain protein 1A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011546"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011563.1">
        <property type="protein sequence ID" value="ENSLOCP00000011546.1"/>
        <property type="gene ID" value="ENSLOCG00000009448.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158135"/>
    <dbReference type="HOGENOM" id="CLU_002479_1_0_1"/>
    <dbReference type="InParanoid" id="W5MT37"/>
    <dbReference type="OMA" id="NDLVFCC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009448">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031445">
        <property type="term" value="P:regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAZ1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
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    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
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        <property type="entry name" value="WHIM2_dom"/>
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    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510:SF1">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WAC" evidence="20">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="19">
        <location>
            <begin position="392"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="1120"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="1398"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="692"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="814"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1174"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="702"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="730"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1185"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1224"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1261"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1280"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1295"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007444"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068253"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1505" mass="169055" checksum="D84553E5A781AF4C" modified="2014-04-16" version="1">EFFERTILCNSLVWSCAVTGKPGLTYQEALESERRARLNLQSFPPPLIPPLLFLTSLTHRSRLHEICDDVYSFVKDRFFLGETVEVTGSTGVRQQCKVLEILPPLQNGVVNGHLSPRMEGDSIVISDDSDEEGGSKTLKSPLLNGKPKTPVKASLFKYKVQPIKTDGCEPFTVKAAQISRKKNLFSRDRLKLLLKQHCSPQNGVIKLKAATIATYRLSEQSFSHFFPDEPPTFTFSPPSKGRGRRPKEHSSPGDLSFTSDGENAAKRKNRDKAALENEKLLKQKEEMKAMALEKAKLKKEKAEAFEAKKREKVDKEKKKEELKKMVEEERLKRKEEKERLKLEKEKEREKLREEKRKYAEYLKQWSKRREDMECDDLKELPIPLPVKTRLPPEQFGDALMVLEFLQAFGELFDLEDEFPEGVTLEVLEEALVGCDPEGPLCELLFFFLSAIFQALAEEQEEVAREQLAEADTKDLTEALDEDADPTQSAISAVASLAAAWPQLYQGCSLKQLDLDSCTLSEILRLHILASGADVTSANAKYRYQKQGGFSSMDDACVELRLGNPALLKKLSGTAVYDLLPGEKLKILHALCGKLLTLVSTRDFIEDSADVLRQAKQELRELKAEQHRREREEAASRIRKRKEEKLKEQELKIKEKLKEDEQRNGTGADAAGENLDGESFRSFLQIVPCPGREEEPEEMDTSTESHEAPRDVATEDEDESELGSKARKTKSSREPPLKESGEDGKEALSPEEEAALRQEQQLKERELLERIQKAAACTYILPLGRDRLYRRYWVFPSVSALFVEEDYSRLTEDMLVPQPVGPPSHSDGAAAPSTPKHLGPGTDPRAAADSSPRDPKPVNRPNLWSFYSSPEQLERLIEALNSRGHRENALKEALLLEKDRITELLRNCPVDRFHVPDLLQTEGRAAPVKSRNAIMSPDSPQSAEKHLETRLKDLLLDIEDRIYQGTLGTVKVMERQAWRTALESGHYELLSPDGKENGVVKTENGEGGAMEIDEANLKVSSKDRLLELKTEGQSAASTSASTPQPVNNTVHYLALALSQIEQGIERKFLKPPLDASDSGRVYKTVLDRWRDSLLCCSSLSQVFLHLSTLDRSIIWSKSILNARCKVCRKKGDAENMILCDGCDRGHHIYCVRPKLKAVPAEDWLCPECRPKQRSRRLSSRQRSSVDSEEEVESEGEENADEEQESEEEEEEEESEEEEEEERSTPHSQQGTPRQTAPTGKGGLKSSGKKGKAAPTSDSKPPLRTSSRTSARLSQEPKPAESSFTDTSSTRLRGRRAAGSAADPAGAPSEGSPQSRHPESQRKKRSLSACSMGGCAQTLEPFLILASFRFILFFFCLFSDLPSKAQVVLTPPASSSRRSSGRSQGVHELSACEQLVVELVRHEDSWPFMKLVSRTQVPDYYDIIRKPIALNVIREKVNNCEYKTASEFIEDVELMFTNCLEYNPRSTNEAKAGVRLQVYFHSQAQKLGLLTKCGDQTPPPPSKRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUX5</accession>
    <name>W5MUX5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01012853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637963.1">
        <property type="nucleotide sequence ID" value="XM_006637900.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUX5"/>
    <dbReference type="FunCoup" id="W5MUX5">
        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012184"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012205.1">
        <property type="protein sequence ID" value="ENSLOCP00000012184.1"/>
        <property type="gene ID" value="ENSLOCG00000009964.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699164"/>
    <dbReference type="KEGG" id="loc:102699164"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182803"/>
    <dbReference type="HOGENOM" id="CLU_033546_4_0_1"/>
    <dbReference type="InParanoid" id="W5MUX5"/>
    <dbReference type="OMA" id="DNLWTEP"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009964">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000011">
        <property type="entry name" value="Putative ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="318" mass="34633" checksum="FEDBEFB6EF9586E4" modified="2014-04-16" version="1">MPNIVLFSGSSHHDLSQKVADRLGLDLGKVVAKKFSNLETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASACRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIAVDNLYAEPAVLQWIKENIPEWRNCIIVSPDAGGAKRVTSIADQLKVDFALIHKERKKANEVHRMVLVGEVKDRVAILVDDMADTCGTVCHAADKLMSAGATKVYAILTHGILSGPAISRINNAAFEAVVVTNTIPQEEKMKVCSKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW02</accession>
    <name>W5MW02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetylserotonin O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Hydroxyindole O-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.4" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01012814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW02"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012561"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012584.1">
        <property type="protein sequence ID" value="ENSLOCP00000012561.1"/>
        <property type="gene ID" value="ENSLOCG00000010259.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161561"/>
    <dbReference type="InParanoid" id="W5MW02"/>
    <dbReference type="OMA" id="ADFSLHM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010259">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017096">
        <property type="term" value="F:acetylserotonin O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000358">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000146">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
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    <sequence length="359" mass="40388" checksum="AA0B497F590C3BDD" modified="2014-04-16" version="1">ADGFSLEKAKMNSVSEIEYPRKILDYMEGFLVSKTLFAACELGVFDFLQAGPPRSAAEVAGGLKTSVDGMERLLAACVGLQLLHAHTQNGEALYSNTELSNIYLTRTSPKSLYHSIQYNSQTIYLCWHYLTDAVREGRNQNEKAFGVSSCDLFDALYRSDEEMRKFMQLMNSVWNICGRDVVTAFDLSPFKTIYDLGGCSGALAKLCVREYPESMVTIFDLPKVVHTAREHFVSSEERRISFHEGDFFKDPLPEADLYILARILHDWTNTRSLELLTKVYRACKPGGGVLLIEALLNEDRSGPLTTQLYSLNMLVQTEGRERTPSEYIGLMATAGFTDIRVQRTGKIYDAILGSYHKRS</sequence>
</entry>
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    <accession>W5MW18</accession>
    <name>W5MW18_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <sequence length="719" mass="83184" checksum="79AFB9282EB303AB" modified="2014-04-16" version="1">NVTVLQVYAPTTDAPEDESECFYAKIEEALEQVPKKDIIYIMGNFNAKVGSQEEANTIGQSGLGERNKAGDCLVKFCHENHLRVSNTWFMQPKCRLYTWTAPNGPHRNQIDYILCSQRWKSSVLAIKTFPGVDCGSDHELLVATIKEKFCNIKNILLVSYAVAVKNRFELLGNEDKQPDELWQEFQSTIIKTAQEHLTYKKLKKRSNWLSAETIKSRSKKSSQSRWGPRRARKDKKAYWDHRCKQMEEECRKGHTRNLFAQVKKIQTSFISFTFTARKGAIKDKNGKVLTGQQCIRSGWREYTEKLYASTSDPEPPHDDPVEIEPAILDEEVVWALKQLPDNKAPGIDCIPAEMLMPVPPVAIKAICQKIWETSRWPKDWKRSVFIPLPKKGDTWDCCNYRTIALIPHVSKVLLKIIQQRLHPIIEAELPDSSDHITNLRWITEKCHEHQKNIYICFIDYSKAFECIELDKLWKALQELHLIQLIRSFYKNQEATVRTHGEIKKVILRIKKESEKMGLHINIKKTKIMTTAGNGEVKITINSEDIECVKVFCFLGSMIERIALGRSAMSSMHQIWKSKDISLTTKCKMVHAIVFPISMYGCESWTLNKADQKRMDAFKLWCWRRLLRIPWTARVTNKEVLERTKPEISLEGKITRLRLTYFGHKAMMLGMVSGTRRGHQRTGWLDAIKTDTGLGIEQLKGKVQDQKAWREIAYRIVESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW94</accession>
    <name>W5MW94_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Ataxin 7</fullName>
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        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <location>
            <begin position="461"/>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="868" mass="93260" checksum="B677DEB30CECBE6A" modified="2014-04-16" version="1">MSERADDDVRGEQRKAARQLRQQQQQQQLQQGEGSTAMATVGERRSLPSPEIMLGQPWSSWVDAAKLHGNDGAESEESLKECGKNREAMRLCREDMPIFGQCPAQDDFYLVMCNHCSQVVKPQAFQAHYERRHGSSSKPPPASTSLSAFPLSTPSSKNNKSGGGGGGSSNSGSGSGSSGRSSSGNGASSNSKILKPAKEKLPSISGNNRPMHMPYSKVPHDKIMTPSVKVEKMPLNARVDGASLKPVPGPTSSTTVSSSIKPGLNCPSIPKPPLLSPGQIPNGKGLLSTPDKKQDDSNISNKRQLHKRLSEREFNPDFHCGVLDLTTRKPCTRSLTCKTHSLSQRRAVLGRRKRFDALLAEHKSKTREKELHRNSECSQQTTPLREPHPPQETHQISHGNNPAESKPSAPSKAKPHNPSLPRPNIYSTHPVGNVSSDPASVHESPHHSTPAAETASRLSSDEGENEEKEDTVDKLDCHYSCYHPRPAAFCSFGSRQFGRSCYAFDRRWDRVRYALSAMVDKHVNSQMWKKIPPVSENTASTAPHKTSTSSLPMSHYGVNATGFLCPSSVMPSPVLVSPPYAQSFDSKSILSYGTTLSARTSPLSSIDHAAYNTQSRQVSSSPQMPSGHSAVPSLLSSRPQKSKPSSKSFRSKEPSATITNCNSSSSSSNNNSSSSSNSSGKKRKNSSPLPAYSSYSAESPSFKKNCAVNSGSSGSMYHASVTSSSHGSVHNTGLNCSTNRTNSLSLKHDQSGRGPPSGSPAESIKRMSVVMKSSDSTLSLGPFVHQSTEQAVNSHSGFSHPHAPMDRLEGKKRKGSPASSSVNSSSSRPNKVAKSPGVNNIHAKHGRTIPGTQGLPNNSLIQQPKARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWF9</accession>
    <name>W5MWF9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012718"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012742.1">
        <property type="protein sequence ID" value="ENSLOCP00000012718.1"/>
        <property type="gene ID" value="ENSLOCG00000010377.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168128"/>
    <dbReference type="InParanoid" id="W5MWF9"/>
    <dbReference type="OMA" id="TEANHFE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010377">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.60:FF:000003">
        <property type="entry name" value="GRIP and coiled-coil domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.60">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032023">
        <property type="entry name" value="GCC2_Rab_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051841">
        <property type="entry name" value="MT-Golgi_org_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902:SF25">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902">
        <property type="entry name" value="NUCLEAR MITOTIC APPARATUS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16704">
        <property type="entry name" value="Rab_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="7">
        <location>
            <begin position="1587"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="676"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1448"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="775"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1344"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1536"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1458"/>
            <end position="1470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1664" mass="191177" checksum="6BF4859B642896CD" modified="2014-04-16" version="1">MEENNLESVASPSTPGTGKSKLDTLSKEDLIKFSKKQVVMTQKLKAKCADLEKEVEELKAKSNSAAGDAVIQEVTERLEAVLLEKAETQQRLVLLRKENEKARQEAQDASEKLSELQNQFDQLTKEQLRKTEVLSKELDTTKEKHKDEIERLQKMLKDSVAEMENLTAQPNKRLCQEDEIRVLKEHLEVSRRGYEEKLSHLQHQLDLAEEERKKEVERLIEAHEAAQTESQKKIEHLQREILQIRKAHQEEAKELMDQVEAAAEEYEKQRDKLLQLQEQLTEQLAQQESSLQDVQEEEETGKAGDQSHTAPQDSGEEAGEVGQLKAALGVLQSQHTMMQDELTYLSNVKMQLESDLQQLKAEFNLEREELEFKIDELQMCKEESEGVAKTWKEKLQVTNEHWEMALNQHEEELRVLQARHQKELKDLEEHFLCLSERGKEKALAEIQELKGRCDRLSVEKSEAVDNYEQTKEILRSRESELNTMKDQGASAIHELQQKLRAAFNEKDSLLEQINNLEAKVEESQKNANEDLNSSVSHLQEKNEEILSLLHQKESAVQELEERLATLSLDRDSMLSSLKNSEAKLKKAREMCSTEQGKATALQQSLEGHSKNNTELKQKLSEAEASLAAALSDKETVDRQLAILESKLAEMHSEKEQQTSELKALEEELAELKETRAALHEESGQPRSEVPSSSSEEREELEQSLQSTSEERDGLKKGLEEMQHQLSEARRHIVGVFQQDASLETLEHAEEEEAGDISALVRKLLAMVLEEKQSLLLQSREKINQLLVDIEEIKEEGRQQQAELQSLVDDLSKEKTMLKQNLEDVVSDMEGLQRDLVEMKSVTEKVKVENESLLAQIAEATEKIRAKENENVDLRGESQKLSVENTGEKEELQQLLAEKEGLLSRLKADIERLQDSKVSSSAEGINVTQLSEQIANLEKENKEKDERINKIKAVAVKAKKELDNSRKEVQTLKEEMESLKVERDRLSSSVKDIIHGAEGYKNLLAEYDKQTEQLDRERERAEGSERQVAELAKRLQAVALQQEQLKSEKEDLVTRIETLHANMKQLEAQLLEAQKVKSALEKDLEGEKLLKEQKIKDHSAALKELEDLQKQHQKQKHQLQQAVQELELLRKDAQQSTLMDMEIADYDRLVKELNQKISDKESRIRELEEEDQMQRKKQEMLHEEMSSLKSQIDQGEEKTSKMKQLLVKTKKDLADSKKREADQLVLQASLKGELEANQQQLEEYKIKYKVITFKCHALLSTFGHIIVFHYKYYSVILSFILTIHENAFPADSEDCAIPNIFQAVHKSFRYEVIIKGSLLSQWSFSTCHKQREYQERIVEQLRFRLQESQQSLEVSSTELQQLQSEHDTLLERHNKILQETVVKEAELRERLCSLQSENLVLKSEHTQTVSQLSTQNEALRASFREQVRHLQEEHRKTVEVLQHQIRRLESQLLQPKEPNVTSPAPAQQMRKTLQERKAADLPTFDLHSTAREEGEGMETTETESVSSASTHLPSLEQLLNSPDPKTDPFVWQAEPSREELTQKLSTANKSIEHLNSLLHETEATNAILMEQITLLKSEVRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSERQALLPVIHTMLQLSPEEKSKLSAIAQGEEEAAAATRTTGWTSYLHSWSGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXE6</accession>
    <name>W5MXE6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Synaptonemal complex protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXE6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013055"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013083.1">
        <property type="protein sequence ID" value="ENSLOCP00000013055.1"/>
        <property type="gene ID" value="ENSLOCG00000010662.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTHX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003368"/>
    <dbReference type="HOGENOM" id="CLU_1371787_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXE6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010662">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008827">
        <property type="entry name" value="SYCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46918">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46918:SF1">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05483">
        <property type="entry name" value="SCP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23020" checksum="3444B52B6C3A01E0" modified="2014-04-16" version="1">MKKLKSAVDEALKNKQETEITCQHKISEMVTLMEKHKNQYDKNVEEKEAELHQKKKKEIELIAAKTSLEQQLSKLKVEFSNLKQQLNSEMKEKKTDPQDLNSTASTSTKETPSQKSAKNSVSEILRKAEIKYSSTKHLSWTPVKKVKNLSTPDIKNFPREIDSFKSLYSSDAHALHLCGEEFKMVSFYYIKLFLNTITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYH1</accession>
    <name>W5MYH1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Plexin-A2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198176.1">
        <property type="nucleotide sequence ID" value="XM_015342690.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MYH1">
        <property type="interactions" value="840"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013430"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013459.1">
        <property type="protein sequence ID" value="ENSLOCP00000013430.1"/>
        <property type="gene ID" value="ENSLOCG00000010923.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694000"/>
    <dbReference type="KEGG" id="loc:102694000"/>
    <dbReference type="CTD" id="5362"/>
    <dbReference type="eggNOG" id="KOG3610">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244850"/>
    <dbReference type="HOGENOM" id="CLU_001436_2_0_1"/>
    <dbReference type="InParanoid" id="W5MYH1"/>
    <dbReference type="OMA" id="KYNEQLM"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010923">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001763">
        <property type="term" value="P:morphogenesis of a branching structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003403">
        <property type="term" value="P:optic vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01181">
        <property type="entry name" value="IPT_plexin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000339">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000695">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000032">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF37">
        <property type="entry name" value="PLEXIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1245"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="18">
        <location>
            <begin position="27"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1271"/>
            <end position="1298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1901" mass="212450" checksum="8359CF43B7A510D6" modified="2014-04-16" version="1">MENHSYMQRTFLCFPKTRVWPLRVYLLVSLMLSSKCCSSQSNFISFHSENRDWAFNHLTVHQKTGSLYIGAVNRVYKLSGNLTLLVSHNTGPEDDNKSCYPPLIVQPCTEPLTPTNNVNKLLLIDYSQNRLLACGSLYQGVCKLLRLDDLFILVEPSHKKEHYLSSVNETGTMYGVIVPSQGEDGTLFIGTAVDGKQDYFPTISSRKLPKDPESSAMLDYELHSDFVSSLIKIPSDTLALVSHFDIYYVYGFASGNFVYFLTVQPETPENSISSGGSSSDLFYTSRIVRLCKDDHKFHSYVSLPMGCVRNGVEYRLLQAAYLSKPGEVLAASLNISAQDDVLFAVFSKGQKQYRRPPDDSALCVFTIMDINARIKERLQSCYQGEGNLELNWLLGKDVQCTKAPVPIDDTFCGLDINQPLGGSRLVSGLTLFTESRDRMTSVASYVYNGYCVAFVGTKSGRLKKIRVDGPPHGGVQYETVSVIKDGSPILRDMAFSMDRNYLYVMSERQVTRVPIESCEQYSTCGECLSSGDPHCGWCVLHNMCSRKDRCERAEEPFRFAATISQCVKVTVHPDSIAVSEHSVPLVVRVSDVPDLSAGITCSFGNLTEVEGQVIGNEILCVSPSSKDVPLIPTDQDWFGVELRLNSRETGQMLTSTEFKFYNCSVHQLCLSCVNSAFRCHWCKYRNLCTHDPSSCSFQEGRVNASEDCPQLVRSEEVLIPVGEVKPITLKARNLPQPQSGQRGYECVLHIQGTSHRVPALRFNSSSVQCQNSSYIYEGMKISDLAVDFSVVWNGNFIIDNPENIKVHLYKCAAQRDSCGMCLKAERKFECGWCSGEGRCTLRQHCLPHANRWLDLSSKNVKCTNPRITEVTPVAGPPEGGTRVTIHGVNLGLAFSEIKGNVQVAGVPCTPQEDGYIIAEQIVCEMNQTSPDTEPGPVQLCIGECRPELKTKSSQLYSFVMPSVQGVSPSRGPESGGTKVTIMGENLGAGSSVTVLFGNQTCEFYGRTMTEIVCYSAPSLTGVSPVQITVGVDRAQVRESLQFEYIEDPTVQRIEPEWSIASGHTPLTVIGTNLDVVQEPRIRVKYSGRESVNVCKVLNSTTMTCLAPSLTTEYRPGLDAVERADEFGFIFNNVQALLIYNNTNFIYYPNPSFEPLSVTGILEQKPGSPIILKGRNLVPPASGGAKLNYTVLIGETPCSVTVSETQLLCEPPNLTGQHKVLVQVGGLHVSPGSVHVLSDSLLTLPAIVSIAAGGGLLLIIVIIVLIAYKRKSRENDLTLKRLQMQMDNLESRVALECKEAFAELQTDINELTSDLDRAGIPYLDYRTYAMRVLFPGIEDHPVLRELEVPGNGQLSVEKALKMFAQLINNKVFLLTFIRTLELQRSFSMRDRGNVASLIMTALQGKLEYATDVLKHLLSDLIDKNLESKNHPKLLLRRTESVAEKMLTNWFAFLLHKFLKECAGEPLFMLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIEYKTLILNCVNPDNENSPEIPVKVLNCDTITQVKEKILDAVYKNMPYSQRPRAVDMDLEWRQGRMARVVLQDEDITTKIENDWKRLNTLMHYQVSDRCVVALVPKQTSSYNIPPSASISRTSISRYDSTFRYTGSPDSLRSRAPMITPDLESGVKVWHLVKNHEHGDQKEGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETLFSTVHRGSALPLAIKYMFDFLDEQADKHSIHDTDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKSSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPNYKSWVERYYADISRLPAISDQDMNAYLAEQARLHSNEFNMLSALNEIYSYVSKYSEEITAALEQDEQARKQRLAFKVEQLIGAMSLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYW1</accession>
    <name>W5MYW1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase 4, N6-adenosine</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYW1"/>
    <dbReference type="SMR" id="W5MYW1"/>
    <dbReference type="FunCoup" id="W5MYW1">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013570"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013599.1">
        <property type="protein sequence ID" value="ENSLOCP00000013570.1"/>
        <property type="gene ID" value="ENSLOCG00000011054.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682782"/>
    <dbReference type="KEGG" id="loc:102682782"/>
    <dbReference type="CTD" id="64863"/>
    <dbReference type="eggNOG" id="KOG2356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016237"/>
    <dbReference type="InParanoid" id="W5MYW1"/>
    <dbReference type="OMA" id="PLVQCGR"/>
    <dbReference type="OrthoDB" id="61116at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011054">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF4">
        <property type="entry name" value="N(6)-ADENINE-SPECIFIC METHYLTRANSFERASE METTL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="482" mass="54893" checksum="DDD78F4D62ADF787" modified="2014-04-16" version="1">MCCFNFLSTQSFSKIQSPSWHLSINEKHSSVPLPVIGLCCNMSVVLKNTKGWLLDQCSYINKSFQQCLSFISPQQKRNLRCRFKRKYFEILKPHILSKSLTNSDCLALGGAETVAQEERKITANSQKVSDEELPSITTKKRKRKHKELNQGESDLQVYHEKVRKVILEGSRALLDEGFTCGYFYSDKANEGSESVSRPSAGSRLADLCEMAKELPFATENEETSVQTVGEGANSTEHLDLFTRVTENNMDCSRLVTLMGEQYLLPPQCSFLLSDMSKMQPLLNYQKYDVIVLDPPWENKSVKRSNRYSFLPASQLKRIPVPGLSAPGCLVITWVTNRQKHLRFIREELYPHWSVEVLAEWLWVKITNSGEYVFPLDSPHKKPYEILVLGRYSGGKESSLRSSEKQEIPVPDQKLIVSVPSTLHSHKPSLADVLKEYVDPAAKCLELFARNLQPGWTSWGNEVLKFQHLSYFNVEETDPDILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZA8</accession>
    <name>W5MZA8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interleukin-2 receptor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">High affinity IL-2 receptor subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">p70-75</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZA8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013746.1">
        <property type="protein sequence ID" value="ENSLOCP00000013717.1"/>
        <property type="gene ID" value="ENSLOCG00000011165.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102688112"/>
    <dbReference type="eggNOG" id="ENOG502S0MR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000049239"/>
    <dbReference type="HOGENOM" id="CLU_541781_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZA8"/>
    <dbReference type="OMA" id="ANQGYFW"/>
    <dbReference type="OrthoDB" id="9419853at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011165">
        <property type="expression patterns" value="Expressed in bone element and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003531">
        <property type="entry name" value="Hempt_rcpt_S_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040951">
        <property type="entry name" value="IL2RB_N1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF22">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18707">
        <property type="entry name" value="IL2RB_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01355">
        <property type="entry name" value="HEMATOPO_REC_S_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866922" description="Interleukin-2 receptor subunit beta" evidence="17">
        <location>
            <begin position="29"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="132"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="398"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="420"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="554" mass="61079" checksum="A5DB2AE3CEFD515D" modified="2014-04-16" version="1">MAMNRNERVVLGMMALRLLPLLVVLAAAEVSSHVSPAVPSLTCTNDYINNISCEWRSSEAYSNTSCRVHLVRRRKQFQCDLIASDEPTVCSCSIQVTKNANTQSFTSVDECDIEVRCGDSKEPAAKIKDFKPSENVRMRPPDAPIIVNSTVSWGAGERVSRLIYEYEFQIKYKRADQTWEEAESRVVVNKENSTQLNENMLEKGALYQVKVRVRPAAKGPLKGVWSHWSPVSEWTSTVGIVKPAVKRAYNPGSNDKEIMDITLAVAIPAVIAVFLTICICGIWSPKWSYKCLHVPDPSKYFDPLNSVHGGNFQKWLSPTFSPESFDTLNSEPVSPVEVFEAKDTTSLFKKAYPSLQEHWDSSGQSSCFSNMGYFYSKHPSSYEIESCSVYFSYQPAEFDSEEGGSGDGGDTPIQTSSSYERLDPSSREQSDPEHPDSGCGMERGEEGDQETEDGREGGEEANPSLLGPGCPPPPFNPAFLPAFPPPFRLPGSHPQLLAHPPFPFPLPGFDFRAFGSGQALDSALSKASSALIQPSGGGYLSVKDVQNRYCNKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0L1</accession>
    <name>W5N0L1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vitamin D-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Gc-globulin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Group-specific component</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Interacts with LRP2; the interaction is required for renal uptake of GC in complex with 25-hydroxyvitamin D3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0L1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014170"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014199.1">
        <property type="protein sequence ID" value="ENSLOCP00000014170.1"/>
        <property type="gene ID" value="ENSLOCG00000011536.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628276_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0L1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011536">
        <property type="expression patterns" value="Expressed in liver and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005499">
        <property type="term" value="F:vitamin D binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="1.10.246.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000264">
        <property type="entry name" value="ALB/AFP/VDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020858">
        <property type="entry name" value="Serum_albumin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014760">
        <property type="entry name" value="Serum_albumin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000213">
        <property type="entry name" value="VitD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11385">
        <property type="entry name" value="SERUM ALBUMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VITAMIN D-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00273">
        <property type="entry name" value="Serum_albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00804">
        <property type="entry name" value="VITAMNDBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48552">
        <property type="entry name" value="Serum albumin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51438">
        <property type="entry name" value="ALBUMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0848">Vitamin D</keyword>
    <feature type="domain" description="Albumin" evidence="13">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="78" mass="8747" checksum="B22A5AF32A477246" modified="2014-04-16" version="1">QLFGVGGKPYEKERICQEYADFGKETFKAIGVILYSQKFSTGTFEEINAVTDEMVKLADKCCAEEASPDCYDTVVFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1S2</accession>
    <name>W5N1S2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Even-skipped homeobox 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EVX1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the even-skipped homeobox family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006636064.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5N1S2">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014581"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014610.1">
        <property type="protein sequence ID" value="ENSLOCP00000014581.1"/>
        <property type="gene ID" value="ENSLOCG00000011857.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686156"/>
    <dbReference type="KEGG" id="loc:102686156"/>
    <dbReference type="CTD" id="2128"/>
    <dbReference type="eggNOG" id="KOG0844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_045075_1_0_1"/>
    <dbReference type="InParanoid" id="W5N1S2"/>
    <dbReference type="OMA" id="NCISPRP"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011857">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072498">
        <property type="term" value="P:embryonic skeletal joint development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:ventral spinal cord interneuron specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000256">
        <property type="entry name" value="Even-skipped homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF2">
        <property type="entry name" value="HOMEOBOX EVEN-SKIPPED HOMOLOG PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="230"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="232"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="103"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="452" mass="48833" checksum="54D5CEFEA741AC2A" modified="2014-04-16" version="1">MGAVTSGPIMTTAPLRMTARKNGAMVIPPRACAAQWSLRIAGSAHPPRCLCVPVQSLFSLRLGVHTTSIMENRKEVVMFMEGGQLGTLVGKIGSNLSEAVGSPVQEPQEKMVHRNCLSPRSAPLSRERGDEEVEDVRSGVCSDIRPGGVVSSPGKPQTDLLSNKGHHSSSDTESDFYEEIDVSCTPDCTSGSMDYSNSKGQSGDSSSNSTGNSEAGKGSASQAAMACGADQMRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMTWPHPADPAFYTYMMSHAAATGNLPYPFPSHLPLPYYSHMGVGAGSTSAAPFSSPLRPLDTFRVLSHPYPRPELLCAFRHPSLYPTPGHGLGGAGGSPCSCLACHSAQPNGLSQRSSGADFTCSSTSRTDSFLTFTPSVLSKSSSVSLDQREEVPLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1Y3</accession>
    <name>W5N1Y3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gastrin-releasing peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GRP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2015" name="Mol. Biol. Evol." volume="32" first="2803" last="2817">
            <title>Prevertebrate Local Gene Duplication Facilitated Expansion of the Neuropeptide GPCR Superfamily.</title>
            <authorList>
                <person name="Yun S."/>
                <person name="Furlong M."/>
                <person name="Sim M."/>
                <person name="Cho M."/>
                <person name="Park S."/>
                <person name="Cho E.B."/>
                <person name="Reyes-Alcaraz A."/>
                <person name="Hwang J.I."/>
                <person name="Kim J."/>
                <person name="Seong J.Y."/>
            </authorList>
            <dbReference type="PubMed" id="26337547"/>
            <dbReference type="DOI" id="10.1093/molbev/msv179"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bombesin/neuromedin-B/ranatensin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="KT235776">
        <property type="protein sequence ID" value="ALD51559.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015194936.1">
        <property type="nucleotide sequence ID" value="XM_015339450.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1Y3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014642"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014671.1">
        <property type="protein sequence ID" value="ENSLOCP00000014642.1"/>
        <property type="gene ID" value="ENSLOCG00000011909.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107076065"/>
    <dbReference type="KEGG" id="loc:107076065"/>
    <dbReference type="CTD" id="2922"/>
    <dbReference type="eggNOG" id="ENOG502S4DG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174898"/>
    <dbReference type="HOGENOM" id="CLU_144892_0_0_1"/>
    <dbReference type="OMA" id="KDMMDYL"/>
    <dbReference type="OrthoDB" id="9879745at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011909">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000987">
        <property type="term" value="P:positive regulation of behavioral fear response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000874">
        <property type="entry name" value="Bombesin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16866">
        <property type="entry name" value="GASTRIN-RELEASING PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16866:SF2">
        <property type="entry name" value="GASTRIN-RELEASING PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02044">
        <property type="entry name" value="Bombesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00257">
        <property type="entry name" value="BOMBESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ALD51559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="153" mass="17372" checksum="F9E10AA1687FBE57" modified="2014-04-16" version="1">MGAEFLFWKYRSVLSFIFLIVIVYRVNFASSFPVENGAPLAKMYPRGSHWAVGHLMGKKSIDFPLGYEEGDGTLYLSTGEEAKELDRPLKWSELIKIMIRALDGNNSQMGQLLEEDIPFSSKNAWEARDKSSNLKEVTNYLLQALTMKDNNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2L3</accession>
    <name>W5N2L3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2L3"/>
    <dbReference type="FunCoup" id="W5N2L3">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014872"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014901.1">
        <property type="protein sequence ID" value="ENSLOCP00000014872.1"/>
        <property type="gene ID" value="ENSLOCG00000012065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688267"/>
    <dbReference type="KEGG" id="loc:102688267"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157423"/>
    <dbReference type="InParanoid" id="W5N2L3"/>
    <dbReference type="OMA" id="YSHFPIW"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012065">
        <property type="expression patterns" value="Expressed in larva and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015106">
        <property type="term" value="F:bicarbonate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000020">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001717">
        <property type="entry name" value="Anion_exchange"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF12">
        <property type="entry name" value="BAND 3 ANION TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00165">
        <property type="entry name" value="ANIONEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00219">
        <property type="entry name" value="ANION_EXCHANGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00220">
        <property type="entry name" value="ANION_EXCHANGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="444"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="713"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="755"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="811"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="837"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="907"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="14">
        <location>
            <begin position="112"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="14">
        <location>
            <begin position="251"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="13">
        <location>
            <begin position="416"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="961" mass="107949" checksum="B4CDD4104F66F770" modified="2014-04-16" version="1">MASRESPGAGQGLGDYQDDEKFHQTLVGTSFEDSILDPEADNINDEDDELKYQQTAQRKPTPATQDQDGSFEGSPFPQDPTGTQMEYAGQNPGVSRKLFSDPRQQNPRGNCQAYVELNELIMGKNQEQYWQETGRWIRYEESFDQTTENWGKAHIPYLTFKSLLDLRKTMTKDTVLLDIEERSLAGIAKQVVDKLVSENEIQDRDREALLRALLLRRSQTLEAQHKEEEPLTKDPQIEMETFSVTKERGSTQNVEATLVLVGAVEFLQKPTVAFVRLKEAVELDAMLEVNVPVRFLFVLIGPSQPNMEYHEIGRAISALMADRVFNSAAYLAEEKKDLTKAIAEFLDCSIVIPPCEIQNEEMLRSIVTFQKQLLKSRFRPPESELEMEAGEKLLKAAALKLEEETPADDPLKRTRRPFGGLIKDIKRRYPRYLSDITDALNPQVLAAVIFIYFAALSPAITFGGLLSEKTNRMMGVSELLVSTAVQGIIFCILGAQPILVLGFSGPLLVFEEAFYSFCKSQGMAYIVARVWVGFWLLLIVLIIVACEGSFLVRYISRFTQEIFSILISLIFIYETFSKLVKIFQAHPLLWKYDDPDANLTAVATTISTGNVTATHIPPKVYPNTALLSLVLMAGTFFLAFFLRKFKNSRFLPGKIRRIIGDFGVPISIFIMVAVDFSISDTYTQKLSVPKGLSVSNPQARDWFINPMGKNNDFPIWMMFAAAIPALLVFILIFLESQITTLIISKPERKMVKGSGFHLDLLLVVAMGGISALFGVPWLSATTVRSVTHANALTVMSKGGKPVIEKVLEQRISGLLVALLVGLSILMEPILKLIPLAVLFGIFLYMGVTSLNGIQLYDRMLLLLVPRKYHPDEAYIHWVKTWRMNLFTFIQIVCLGALWGVKSSPASLALPFVLILTIPLRMLMHGRLFTDVEMKCLDADDAKVNFEEKEGEDVYDETAMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3K8</accession>
    <name>W5N3K8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.222</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70511"/>
            <dbReference type="Rhea" id="RHEA-COMP:17919"/>
            <dbReference type="Rhea" id="RHEA-COMP:17920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="ChEBI" id="CHEBI:189633"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70531"/>
            <dbReference type="Rhea" id="RHEA-COMP:17922"/>
            <dbReference type="Rhea" id="RHEA-COMP:17923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="ChEBI" id="CHEBI:189634"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.222" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01000341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3K8"/>
    <dbReference type="FunCoup" id="W5N3K8">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015217"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015246.1">
        <property type="protein sequence ID" value="ENSLOCP00000015217.1"/>
        <property type="gene ID" value="ENSLOCG00000012361.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684219"/>
    <dbReference type="KEGG" id="loc:102684219"/>
    <dbReference type="CTD" id="5986"/>
    <dbReference type="eggNOG" id="ENOG502QV30">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160801"/>
    <dbReference type="HOGENOM" id="CLU_056611_0_1_1"/>
    <dbReference type="InParanoid" id="W5N3K8"/>
    <dbReference type="OMA" id="DTDWCPM"/>
    <dbReference type="OrthoDB" id="8959630at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012361">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033829">
        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035284">
        <property type="term" value="P:brain segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021592">
        <property type="term" value="P:fourth ventricle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021654">
        <property type="term" value="P:rhombomere boundary formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000003">
        <property type="entry name" value="Beta-1,3-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="21">
        <location>
            <begin position="139"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="45535" checksum="6B357981A4E8E4BF" modified="2014-04-16" version="1">MHLSRVGISKLCFVLSLAFCAFLLLLIPGLQPPQRPVDPPQPRPHGRTKAGWEGGVGQPPSLSGALEKAHEAQVGNPRTRGHGAKGETKDVDTLGGQAQTPKAKSSALINMAVGHKDSAPFSGSNSLHDLHGLPRPRDRLELGDIFIAVKTTRKYHKSRLNLLFQTWISRAKDQTYVFTDGEDKELRKKAGSHMINTNCSAAHTRQALCCKMSVEYDKFIESQRKWFCHVDDDNYVILPSLMRLLSSYSHTQDVYLGRPSLDHPIEAPERVKSDGLTSVKFWFATGGAGFCISRGLALKMSPWASLGNFISTAEKIRLPDDCTIGYIIEALLEVRMTHTRLFHSHLENLQRLPTDTVLGQVTLSYGGYENRRNVVSIGGGFSLAEDPTRFKTVHCLLYPDTEWCPSKTHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N424</accession>
    <name>W5N424_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4E binding protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF4E-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N424"/>
    <dbReference type="FunCoup" id="W5N424">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015383"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015412.1">
        <property type="protein sequence ID" value="ENSLOCP00000015383.1"/>
        <property type="gene ID" value="ENSLOCG00000012500.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S03G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163328"/>
    <dbReference type="HOGENOM" id="CLU_111706_0_0_1"/>
    <dbReference type="InParanoid" id="W5N424"/>
    <dbReference type="OMA" id="KQAKSCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012500">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008606">
        <property type="entry name" value="EIF4EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669:SF5">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05456">
        <property type="entry name" value="eIF_4EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="91" mass="10028" checksum="5B9D13AE88B0A6EA" modified="2014-04-16" version="1">ANCQKSKSCPIPTRVMHLKDWSQLPDCYSQTPGGTLFSTTPGGTRIIYDRKFLLDCRNSPVARTPPCFLPQIPGVTVPALAPLSKLQELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4E4</accession>
    <name>W5N4E4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4E4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015503"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015532.1">
        <property type="protein sequence ID" value="ENSLOCP00000015503.1"/>
        <property type="gene ID" value="ENSLOCG00000012593.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164992"/>
    <dbReference type="HOGENOM" id="CLU_004343_3_1_1"/>
    <dbReference type="InParanoid" id="W5N4E4"/>
    <dbReference type="OMA" id="WEAFGKF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012593">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035525">
        <property type="term" value="C:NF-kappaB p50/p65 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000004">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF9">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B P105 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="12">
        <location>
            <begin position="13"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="360" mass="40795" checksum="D77871595A469A66" modified="2014-04-16" version="1">MNYLELSPNTTHEPCKHIKIIEQPRQRGFRFRYGCEGPTHGGVQGASSEKNKKTFPAVEICNYTGPARVVVQCVTVDDPPLLHVHSLVGKQCENGMCVVGVSGPDMTVSFPNLGILHVPRRSVVDILEERLVQGWRREHCKELQHRDPTDRERDRLHRAAQQCARALDLTVVRLMFTVYLPDNNGNFVQALQPAVSSPIYDSKAPNASSLKIVRMDRTSGSAAGGEEVFLLCDKVQKDDIQVRFYQENDDGTCWEAFGKFGPTDVHRQYAIVFKTPRYFDPYITQPTSVFVHLRRRSDGETSEAKPFIYFPQKTGKERSHRKRKNPDTAEHDRSRVSNGHLQGYYCSIPDFTYTPAVTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6D2</accession>
    <name>W5N6D2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phosphodiesterase</fullName>
            <ecNumber evidence="19">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="19">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01001996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6D2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016191"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016221.1">
        <property type="protein sequence ID" value="ENSLOCP00000016191.1"/>
        <property type="gene ID" value="ENSLOCG00000013138.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682301"/>
    <dbReference type="eggNOG" id="KOG3689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155475"/>
    <dbReference type="InParanoid" id="W5N6D2"/>
    <dbReference type="OMA" id="PIWLPLA"/>
    <dbReference type="OrthoDB" id="74705at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013138">
        <property type="expression patterns" value="Expressed in intestine and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141162">
        <property type="term" value="P:negative regulation of cAMP/PKA signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000003">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000004">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000015">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5" id="KW-0140">cGMP</keyword>
    <keyword evidence="12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="10 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDEase" evidence="21">
        <location>
            <begin position="557"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="634"/>
            <end position="638"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="785"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="785"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="838"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="889" mass="101561" checksum="90F8247B2739CFD6" modified="2014-04-16" version="1">MEDFCLSENERMEAWLDDHLDFAHSYFVRKATREMVNAWFAERVHSIPPSKEGSRAQAEPGLVHQSGRQETATPGTPTRKISASEFDRPLRPIVCKDSEGSLTFVSDSEKVQVPLKAQRLQAGEVQVSDQCTRLLELVKDISSHLDVTALCHKIFLHINALIDADRYSLFLVCEDSSNEKFLVSRLFDLAEGSTLEEASNNCIRLEWNKGIVGYVAATGQPLNIKNAYEDARFNAEVDHITGYKTQSILCMPIKNHREEVVGVAQAINKKCGKNGTFTEQDEKDFSAYLAFCGIVLHNAQLYETSRLENRRNQVLLDLASLIFEEQQSLEVILKKIAATILSFMQAQGCTVFIADGDSMNSFSSVFHMEYEELGESPEVPKRESGNMLIHKTNQCNFHMHDNVCIFRTESVRPTVSETVYRRDCDVSKINYMYAQYVKNTMEPLNIRDVTKDKRLPWTNENTENSSHQIKSLLCTPIRNGKKDKVIGVCQLTNKMDEASGKIKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQVVTLEVLSYHASAAEEETQELQVTAAATVPSAQSLGLLDFSFSDFDLSDVETTQAAIRMFTDLNLLQNFQMKYENLCQWILSVKKNYRKNVAYHNWRHAFNTAQCMFAVLKTGRFQNKLNDLEILALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDQCLMILNSPGNQILSGLSLEEYKTTLKMIERAVLATDLALYMKKRGEFFELTKSNRFTWEDEYHRDLLRSMLMTACDVSAITKPWPVQRRIAELVATEFFEQGDKERRELNIEPIDLMNREKRDKIPSMQVAFIDAICIQLYETLALMSENFLPLLEGCKKNRQNWKLLAEQGEKEINGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N760</accession>
    <name>W5N760_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Ligand for cxcr3.2. Chemotactic for macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N760"/>
    <dbReference type="FunCoup" id="W5N760">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016469"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016499.1">
        <property type="protein sequence ID" value="ENSLOCP00000016469.1"/>
        <property type="gene ID" value="ENSLOCG00000013359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S7MM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160757"/>
    <dbReference type="HOGENOM" id="CLU_143902_3_0_1"/>
    <dbReference type="InParanoid" id="W5N760"/>
    <dbReference type="OMA" id="NGREACL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013359">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045236">
        <property type="term" value="F:CXCR chemokine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF198">
        <property type="entry name" value="PLATELET BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041318913" description="Chemokine interleukin-8-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="7">
        <location>
            <begin position="31"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="104" mass="11630" checksum="A6F6AFA2C63FB1F2" modified="2014-04-16" version="1">MNCKMTVIVLFVCLTYAALSEGMSLKSLGTELRCQCINKESRFIHPKLIQNVELIPSGPHCANTEVIATMKTGKKICLEPTAPWVKILIKAIMEKAEYCSDERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7C1</accession>
    <name>W5N7C1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Guanylate cyclase</fullName>
            <ecNumber evidence="2 13">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Photoreceptor outer segment membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2 13"/>
    <dbReference type="EMBL" id="AHAT01002052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7C1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016530"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016560.1">
        <property type="protein sequence ID" value="ENSLOCP00000016530.1"/>
        <property type="gene ID" value="ENSLOCG00000013396.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163785"/>
    <dbReference type="InParanoid" id="W5N7C1"/>
    <dbReference type="OMA" id="QINWTAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013396">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001214">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000019">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF458">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867374" description="Guanylate cyclase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="278"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="645"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="863" mass="96097" checksum="13AE36988DBD681F" modified="2014-04-16" version="1">RSKYSSILIVACYLLLIKAVTAWHDLDNSEYDCWPLEKPDEFNMIDCGGLEMAWVHRPPEKVVSGEEFNVTYAAVAQDSFYEWAVKNDILSHSPVIISNASAAKEFCEGHECPPNWKDANGENCCIHHANIHSCPLAFMVKGGICGPWIPDDGRIFTHTLSAAGKMTQTNWTAKVVLIHVGVTSLIAHIRVGKMQVALEAKTTVLPAIVCGDGVCEEEEICSTCPADCGECPMTASIKVAICLPIAFIFVGFIFIAVWFQYQKQKMFWDESWIIDFSQIKQDQVARTTMGSIMSVPHANSDSNASCMTTLSSCTGGLNPSRKQHFTLTGVYDGRTVAIKKIKKKTFSLTKTLRREVKQVSCARELDHPNLCKFVGGCLEVPNVAIVTEYCPKGSLNDVLLNEEIPLNWGFRFSFATDIARGISYLHQHKICHGRLKSMNCVIDDRWVCKITDYCLGTYRKEDCGEAPSTYQQRLKEVYMPPEAQSSNFEPTLAGDVFSYSIILLEIATRSDPVPAEDSSLECAWCPPLPELITGKAENTCPCPSEYVELIRRCRSHNPAHRPTFDQIKKFVHKINPNKVSPVDMMMNLMEKYSKHLEVLVAERTQDLVHEKQKTDRLLYSMLPKQVADDLRQGKPSQAQSYVSATIFFSDIVGFTQLSSTSTPYQVVEFLNKLYTTFDEIIDNYDVYKVETIGDAYMVVSGVPKENGILHASEIASMALDLVAVCKTFRIPHKPNTLLQIRAGIHSGPVVAGVVGTKMPRYCLFGDTVNTASRMESTSEALKIQCSSGTFYLLEEIGGYILTCRGSLQVKGKGDMVTYWLEGKEAAPVNKETAKTTPAVLEIEKDHYPSIPGCLNNDLHFNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7H3</accession>
    <name>W5N7H3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01024879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628775.1">
        <property type="nucleotide sequence ID" value="XM_006628712.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198610.1">
        <property type="nucleotide sequence ID" value="XM_015343124.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198611.1">
        <property type="nucleotide sequence ID" value="XM_015343125.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198612.1">
        <property type="nucleotide sequence ID" value="XM_015343126.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198613.1">
        <property type="nucleotide sequence ID" value="XM_015343127.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7H3"/>
    <dbReference type="FunCoup" id="W5N7H3">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016582"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016612.1">
        <property type="protein sequence ID" value="ENSLOCP00000016582.1"/>
        <property type="gene ID" value="ENSLOCG00000013450.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687621"/>
    <dbReference type="KEGG" id="loc:102687621"/>
    <dbReference type="CTD" id="5873"/>
    <dbReference type="eggNOG" id="KOG0081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156218"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="InParanoid" id="W5N7H3"/>
    <dbReference type="OMA" id="MHAYTED"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013450">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04127">
        <property type="entry name" value="Rab27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000402">
        <property type="entry name" value="Ras-related protein Rab-27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041837">
        <property type="entry name" value="Rab27a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="223" mass="25089" checksum="AC29547C3F5A1BF9" modified="2014-04-16" version="1">MSDGDYDYLIKFLALGDSGVGKTSFLYQYTDGKFNSKFITTVGIDFREKRVVYKSNGADGIAGRGQRIHIQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWMSQLQMHAYCENPDIVLCGNKCDLEDQRAVKAEEAQELAEKYGIPYFETSAANGQNVAKAVEVLLDLIMKRMERCVDKSWIPDGVLRSNGHSSADGLPEDKTQETGKCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAY3</accession>
    <name>W5NAY3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01014837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAY3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017792"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017824.1">
        <property type="protein sequence ID" value="ENSLOCP00000017792.1"/>
        <property type="gene ID" value="ENSLOCG00000014454.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154089"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="W5NAY3"/>
    <dbReference type="OMA" id="PSPEWDC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014454">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051019">
        <property type="term" value="F:mitogen-activated protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14089">
        <property type="entry name" value="STKc_MAPKAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000094">
        <property type="entry name" value="MAP kinase-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000156">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1170.10:FF:000001">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1170.10">
        <property type="entry name" value="MAP kinase activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027442">
        <property type="entry name" value="MAPKAPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="67"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="399" mass="44638" checksum="25389A4430D8A17C" modified="2014-04-16" version="1">CSTRSGKMSNGNEQEKPPEPQADPPNPTQTPSDGSALSSAVSSPPLFPLPGHPKLEFRRNAVTDDYKVSSQVLGLGINGRVLECSDKRTGDKCALKILYDSPKARREVELHWRVSGGPYIVRILALYENMHHGKKCLLIIMECMEGGELFSRIQVRGDQAFTEREASEIMRHIGTAIHYLHHMSIAHRDVKPENLLYTTKESNAVLKLTDFGFAKETTLHNSLQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRMGQYEFPNPEWADWRPLLSVLSVLRLCPAGVTDCLSVFQQSMVVPPTPLHTTRVLMEDKEMWDDVKEEMTSALATMRVDYDQVKIKDLDTSSNPLLNKRRKKAAAGGGGNTVCNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCE8</accession>
    <name>W5NCE8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec3 PIP2-binding N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCE8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018307"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018339.1">
        <property type="protein sequence ID" value="ENSLOCP00000018307.1"/>
        <property type="gene ID" value="ENSLOCG00000014861.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166719"/>
    <dbReference type="HOGENOM" id="CLU_015381_1_0_1"/>
    <dbReference type="InParanoid" id="W5NCE8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014861">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14683">
        <property type="entry name" value="PH-EXOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="6">
        <location>
            <begin position="191"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1028" mass="115493" checksum="CF9B96150D840078" modified="2014-04-16" version="1">MYTWPPTFSSTISGNAGSSPGGQPLAWSGRAMVGRVRAGKETPSPSLSLSPSLAVCGGDFFGLAELGAPTGKGRQLHRATLGPSGKPREGEVVTPVSPGKRLLIFNFSRRIKPVNGQGVQQSTRLGETPAGHSTSLLLFIAREKSPPGFFSSSLLCRWMAVFSLRSILQREVFTPRGERLLGVSFVWKARRKRKSAILCAAVSSDQSGQVVLVTVKADREHYKLSACWPATELTLVDGKDAVKETAEFHLHLDKVYKWVSSSSGEKTAFVSCLWKINQRYLQDKVKFINISPSILEEVLPWQQGRAVSSQVEEEEDGYQELTAREAADIQYLMEQSELAVSNAKAFTQTLHANLQALDKENLLALLDSERQVGSLVQLLEVGLEEVARIEQSLSHHDSLLRTVQQQMDSIHTRNAWLQRIDRNHGLLDAELSYLVDSLTLSEEHVWVLSEGDLGKERDVKACVAAVRALTNCANTPLTPGQRKLHAVAEQLIKFESLRQIFEQRSSLYINALIVQQDVANTGAAAVDGDRLVLPKLTDFHQQLTQYAPLMDWLRETNPTAFQHLQKVYTDIIGKLYDRQMKDLFDAAKFKVSGVKETKRFGMIPVLQGSMERAPNSSSSSSSAEWRPKRVVEQMLSELSSVLIAEQQFLTIFFALNRGDRGSKHSLSTEYPAVTISNVADQTKLPAGHKKRPCSWIRVSNTAPLVSYGPGSLDCMQELLGCVEPRLSVLLALCEKTEPLSCLWMLKETDQRLHSTLRNPHASFYCSLLFCTLQLSQQSLQGHTEALCKEIENLRLSKKARMGLLPFVPRLEEFVSEGENVLRGSERRWGLDAAYRQLFTAAFQSLESLSTQSLRTHPLVVLLLNFHRLHGFISRLHLPELESLCEEAREKSALYRQQYVTLNLGQPLGTLTEFLGGVRTCLAHGVREEEVCFKLAYSKQELRKVIERHPGREVRKTIETLYKRVSRHLAEDESLLQVVWRAMQDELTEQCRGFEQLIERCYPGARIKLDFTEEELQQYFSSVSFRDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCK8</accession>
    <name>W5NCK8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorting nexin 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015207196.1">
        <property type="nucleotide sequence ID" value="XM_015351710.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCK8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018367"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018399.1">
        <property type="protein sequence ID" value="ENSLOCP00000018367.1"/>
        <property type="gene ID" value="ENSLOCG00000014918.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688657"/>
    <dbReference type="CTD" id="29934"/>
    <dbReference type="eggNOG" id="KOG2527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153609"/>
    <dbReference type="HOGENOM" id="CLU_057172_2_2_1"/>
    <dbReference type="InParanoid" id="W5NCK8"/>
    <dbReference type="OMA" id="NMYTDYE"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014918">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051223">
        <property type="term" value="P:regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07294">
        <property type="entry name" value="PX_SNX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000002">
        <property type="entry name" value="Sorting nexin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF3">
        <property type="entry name" value="SORTING NEXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="28"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="161" mass="18853" checksum="96C227FEFC76E40F" modified="2014-04-16" version="1">MSEPTVADTRRLNSKPQDLTDAYGPPSNFLEIDVYDPQTVGVGRNRFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDEGIFEESFIEERRAGLEQFINRIAGHPLAQNERCLHMFLQEESIDRNYIPGKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCM1</accession>
    <name>W5NCM1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RAB39B, member RAS oncogene family a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Autolysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCM1"/>
    <dbReference type="FunCoup" id="W5NCM1">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018380"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018412.1">
        <property type="protein sequence ID" value="ENSLOCP00000018380.1"/>
        <property type="gene ID" value="ENSLOCG00000014927.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0091">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158132"/>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="W5NCM1"/>
    <dbReference type="OMA" id="XSITRAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014927">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120281">
        <property type="term" value="C:autolysosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04111">
        <property type="entry name" value="Rab39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000358">
        <property type="entry name" value="RAB39B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    <sequence length="217" mass="24965" checksum="D30B904FE7B99AE7" modified="2014-04-16" version="1">MLDNMEAIWLYQFRLIVIGDSTVGKSCLIRRFTEGRFAQVSDPTVGVDFFSRLVEIEPGKRIKLQIWDTAGQERFRSITRAYYRNSVGGLLLFDITNRRSFQNVHEWLEEARSHVQPLQIVFVLVGHKCDLEAQRQVGRQEAEKLAGAYGMRYVETSARDAINVEKAFTELTRDIFELVKRGDITIQEGWEGVKSGFVPNVVHSSEEVMKSDRRCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCP9</accession>
    <name>W5NCP9_LEPOC</name>
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        <submittedName>
            <fullName evidence="21">Adhesion G protein-coupled receptor A2</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG1"/>
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    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869548" evidence="16">
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            <begin position="32"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1358"/>
            <end position="1367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1381" mass="149261" checksum="A9DD3F2FECF88681" modified="2014-04-16" version="1">MRVPGAGVPFCFPLFLLLVLFAVSGSRLAKACSELTTISGCRCNDDRSKSHVTASRKKVSCIGEELTEPPQARLLPNLTVTLILNNNRISVLRNGSFFGLSALEKLDLKNNLISTILPGAFHGLSELRKLDLSNNRIGCLAPEMFQGLSILTKLNLSGNIFSTLEQKIFQELPSLKLVSFDTDFLFCDCNLRWVPGYFRSSSARLSEETLCSYPSSLRGKPLRGLRENQLTCGGPLELHTLSLLPSLRQVVFKGDRLPFQCTAALLDRTTSLHWRHNGQPVTSTPEEGIQLEDSILHDCTLITSELILWNIHVEASGEWECVVSTGRGDASRSVEIVVLENGASFCPEEKVTNNRGEFKWPKTLAGITSYQYCLQYPYPAITINGGVEQKRASRHCNRLGRWEEGDYSQCLYKNDITRVLHTFVLMPINASNALTLAHQLRTYTAEASGFTDTVDVLYVAQMMEKFMEYVGQIRELSEVLVEMGSNLMQVDEQILARAQREESACSSIVQSLESIAKWQLHGNAQDLSMSSRNIAMEAYLIKPSHYTGLSCTAYQRSEGAGAGAGGSHVGQEGAEPSHEQQLRFRCTAGTHNASLHSFPLKNSVALASVSLPPSLFPPAAPPDCKLQFLAFRTGRFFPVTGNSSALADQTWRRSVNTPVIFAGLDGCQMWNESEPVAVSLRHSFLGSAPVAAHWSFSAGDRQGGWSQEGCQLVASDSTVSTLRCAHFSNYAVLQDLPEFPSLAAVPVEVLHPVVYTCTALLLLCLFTVIITYILHHSSIRISRKSWHALLNMCFHIAMTTAVFAGGITLTSYPVVCQAVGIALHYSSLSTLLWIGISARVIYKEAVWQSPRQPEDELPVPPTQRPMLRFYLIAGGVPLIICGITAAVNINNYGDGSPYCWLVWRPSLGAFFVPAGFAVLVSWAYFLCTISCLQGPRHQSHKDLPGSSSSNSSPLPESKPALGGSASLLSTDSVVGAMHTGMALEDQYSLKAQFLAVVTTHVLYVALWSCGALAVWQGGKSQLLFSCMYGVATTVLGIFLLVHHCLRRLDVQTTWLRCCPGYHRSQPIPAYLHPCTAGTGAQTNTSERGSQIFVGCLPPGEPHNSSSARSSSTPSGISSLSGPGPCKLTNLLQVAQDNTNNATRTPSATNTNTSTSTENNVKHPNNNLLSTAGSVAPGQQQRRKVGNRTKQGASGLYHHRGEGRGHYRLKALRGGGGGGSLGALAPLGMEQLTTPQLGHKHASSENGSIHNSQSESHTSPLANGWRVGEVAATSFSEGSDGGSSGSRQKPFPLLPSVASRVATQGAQRRSASRDNLKLAAAAEKEAKRCSFPLNSGNGNFSPGTSQNGTLKGSGLELDTSGTEHSQVSVGMKSGIWKSETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEU4</accession>
    <name>W5NEU4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc-binding protein A33-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEU4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019153"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019185.1">
        <property type="protein sequence ID" value="ENSLOCP00000019153.1"/>
        <property type="gene ID" value="ENSLOCG00000015560.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158537"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_3_1"/>
    <dbReference type="InParanoid" id="W5NEU4"/>
    <dbReference type="OMA" id="DETCKTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015560">
        <property type="expression patterns" value="Expressed in zone of skin"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="320"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="159"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="516" mass="58612" checksum="2F47CA0FFB0D5761" modified="2014-04-16" version="1">ARQYPLRSSMDSAVDELSRLREDLTCPLCLDLFRSPVLRECGHHFCQECVGRCWEGPGESARCPLCGRGAVDEVVQDAPLLGSVAETVREMMGDRREATGKRKREQYCEEHGQKLKLFCQEDGKAICVACGSSQLHQSHSLAPLDEVLGKYREKLGGFLQQIRKEVKKASRSLQTAEDMSAVLKDDRESIEKTIEAEFAELHRFLDREEAAAKAKLRHAEEEKRKALERNRTRSEAEKCRLLQAKELLKDQLALHDDPAAIEKMQRCDFDERVSFTLFQKSDETCKTHYDLKDLLSRKDLTFQKPADEYISLSSEEYVGPLQYAVWKKMKDILTPAPLPVTFDPDTANPHLILSNGNTTAKTGGTRRALAPKPERFDTYLNVLGSRGFISGRHYWEVWVGNKSEWELGVAAASVERKLCYQARPADKVWIIALDRGGSYTAHDFGTVTLRLNSKPRKIGVYVDYGGGRVVFHNAEDMTVIYTFIDIFEEKIYPIFNPGHRRGPKNRKALRIVTPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF26</accession>
    <name>W5NF26_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase NEURL3-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF26"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019235"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019267.1">
        <property type="protein sequence ID" value="ENSLOCP00000019235.1"/>
        <property type="gene ID" value="ENSLOCG00000015625.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107076832"/>
    <dbReference type="eggNOG" id="KOG4625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156696"/>
    <dbReference type="HOGENOM" id="CLU_091737_1_0_1"/>
    <dbReference type="InParanoid" id="W5NF26"/>
    <dbReference type="OMA" id="FKENCAG"/>
    <dbReference type="OrthoDB" id="6078042at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015625">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000005">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF36">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEURL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NHR" evidence="5">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="196" mass="21622" checksum="74C1C6D985266BA0" modified="2014-04-16" version="1">MGQGTFKENCAGASLRRCSRRCLGPLTFHPHAKGSSISLRAGGLLAERVGCSFKDAIVFTSRPLRVGEVARLQVERGEGHWHGALRLGVASWHPSHLDPDLLPPFACPDLQQLPGFWVEPLPEECASPGNELAFWVTRRGELHFQVNGGCKHKLLGGIDISAPLWGLIDVYGQTRALWMLGKYKDGYFTRTDHVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGA7</accession>
    <name>W5NGA7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tetraspanin 33a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGA7"/>
    <dbReference type="FunCoup" id="W5NGA7">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019666"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019698.1">
        <property type="protein sequence ID" value="ENSLOCP00000019666.1"/>
        <property type="gene ID" value="ENSLOCG00000015974.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159484"/>
    <dbReference type="InParanoid" id="W5NGA7"/>
    <dbReference type="OMA" id="LVINTMC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015974">
        <property type="expression patterns" value="Expressed in mesonephros and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF154">
        <property type="entry name" value="TETRASPANIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="284" mass="32081" checksum="233A48041532DA71" modified="2014-04-16" version="1">MGRRRIDTYKEDFTFVSRIVKYLLFIFNMIFWIISLVMISVGVYARLMKHAETAMACLAVDPAILLMIIGILIFLITFCGCIGSLRENICLLQTFSICLTIIFLLQLAAGILGFVFSDKARGKVTEVINNAIVHYRDDLDLQNLIDYGQKEVSGTCKGAQGFIFSSMAGYFCYETDENRYYVCSRRICCLSVGHVVEAVINTMCGQGMQDLNYQQAEAFIHTNGCIDKLVNWIHSNLFLLGGIALGLAIPQLVGILLSQILTNQIKDQIELQKYNMQHRSDPWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGM4</accession>
    <name>W5NGM4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G patch domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGM4"/>
    <dbReference type="FunCoup" id="W5NGM4">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019783"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019816.1">
        <property type="protein sequence ID" value="ENSLOCP00000019783.1"/>
        <property type="gene ID" value="ENSLOCG00000016058.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688285"/>
    <dbReference type="KEGG" id="loc:102688285"/>
    <dbReference type="eggNOG" id="KOG0154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000025698"/>
    <dbReference type="HOGENOM" id="CLU_041240_2_0_1"/>
    <dbReference type="InParanoid" id="W5NGM4"/>
    <dbReference type="OMA" id="HSWETGH"/>
    <dbReference type="OrthoDB" id="6095487at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016058">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="461"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="55343" checksum="0EAFA8F33FB4B8BC" modified="2014-04-16" version="1">MFRAANSRSLGQVETSWHFCRTMDELVHDLVSALEESSEQARGGYAEAGDHSLSVGCLLKRQARKRRGRKRRSDNACPPWEPSHYLSEGSESSLEEQHGKDYRSASHSKDHSDSDDQLLGAKRRLTSVLEAGRAKRPMWHEDPVAEGLGSRSLRRRRKVKRMAVDPPAEVASVMTLTKQQGSASQELPPQEASTKPPEHGKSKLKKRKLTASRHGQEAADEGVVVESEDTGRANKDKMDYEDQKGSDEEMSDSETSSLSNSSDGGLYTNDEGRQGDDEQSDWFYEGEPGGACGIAGVIPWWEKEDSSDLERELSDPVFNSILTGSFPLMSKEARGGFQARLNHLHGVPSGMNRATVQQSAGKISTPGPGCNERLARFSQDPHHHDSWFSPGARREHSQLVWDARTDRGHRKNCSLKTASRQTSVHLGSLCTGDIKRRRKAAPMGGPTTTGFVGENAPPISENNVGNRMLQSMGWTPGMGLGPDGKGIAEPIRALQRPKGAGLGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHV9</accession>
    <name>W5NHV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptotagmin like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020218"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020252.1">
        <property type="protein sequence ID" value="ENSLOCP00000020218.1"/>
        <property type="gene ID" value="ENSLOCG00000016375.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160610"/>
    <dbReference type="InParanoid" id="W5NHV9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016375">
        <property type="expression patterns" value="Expressed in bone element and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08521">
        <property type="entry name" value="C2A_SLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF1">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RabBD" evidence="5">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="395"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="558"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="710" mass="81172" checksum="EE886C70BB549C34" modified="2014-04-16" version="1">YNSLAGNMDLSFLKELEREKVLEVLQRDKILRALEEERIRKLKSELQELRRKGAKSFSQHYSEKSCARCQRPLGKLWNCGAVCKGCSHRICSKCRIAITARVWKCTVCYAYREVKIRSGEWFLEERAKKFPIEAGRDAMDISIKYKIADKQETVGEKLLKSYQRLRRGLFVISIKHCITIFYPYFFRSQKLRQRLEDTGMGEPDCCCCSSLLHIHLLEQTWSKNSKGFTRSVENLFLSLTTHMKMNCITQNKNRILWTQSSDVNRTPELKMSSCVHFVFQDNVNSSNFFFIKATLFPSVPVDCGLRTQFFFLATVTLLQSHVLSVVKKLTKVPSLPILSKKTDNKEDLKSNVNEGRPTSHLYSEEQSACCFLSKESICSISSTCTELGNFDKASVTGEIELSIAYNFKLSCLEISVRACKNLAYGDTKRKRCNPYVKIYLLPDKSPHSKLKTAVKKNTVDPVFNETFRYNIERSQLETRTLQASLWHSGTLKRKVFLGEVLISLDCWKFEESDYLVKVVISDQPDRYEECVPEQYNGELMIKVKFSSLPQGSGIHFGKEGKTILVTENQIQMTDLGQLSVLIIGAKNLPSLRPDGTVNSFVKGCLILPGGREIKQKTPLSKRKACPQWNHPMVFSGIYQAEVMDSSLELSVWDSAPFGRPDRFLGGVRLEKDTAGMSFSSGLPWQQLLRRPNTWHDFTLIIQPNANMTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK16</accession>
    <name>W5NK16_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dermatan sulfate epimerase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatan-sulfate isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011995">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK16"/>
    <dbReference type="FunCoup" id="W5NK16">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020975"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021011.1">
        <property type="protein sequence ID" value="ENSLOCP00000020975.1"/>
        <property type="gene ID" value="ENSLOCG00000016968.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPWZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006522"/>
    <dbReference type="HOGENOM" id="CLU_308813_0_0_1"/>
    <dbReference type="InParanoid" id="W5NK16"/>
    <dbReference type="OMA" id="FFIHAVC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016968">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047757">
        <property type="term" value="F:chondroitin-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050654">
        <property type="term" value="P:chondroitin sulfate proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050655">
        <property type="term" value="P:dermatan sulfate proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.70:FF:000001">
        <property type="entry name" value="dermatan-sulfate epimerase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052447">
        <property type="entry name" value="Dermatan-Sulfate_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032518">
        <property type="entry name" value="HepII_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532:SF3">
        <property type="entry name" value="DERMATAN-SULFATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16332">
        <property type="entry name" value="DUF4962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48230">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="907"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="940"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="Heparinase II N-terminal" evidence="10">
        <location>
            <begin position="162"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="965" mass="110108" checksum="0DD9568E21B46A5D" modified="2014-04-16" version="1">WSGPTSDVRMRTYTRGAPTVFFITLFCFSLALSLEEDPSGGIPFLGGNYDGHPMLYFSREEVGALQRQGRGTHLHIAAKIQEAGRTMLAHPEEYLPPWSPADFSARWNEIYGNNLGVLSMFCVLFPETERALDLAREYMERMAAQPSWLVKDAPWDEVPLAHSLVGFATAYDFLYGDLSRSQQERFLQVIANTSRYMYEKSYNRGWGFQYLHNHQPTNCVALLTGSLVLMNQGYLQEAYLWTKQVLAIMEKSLVMLQDITDGSLYEGVAYGTYTSRSLFQYMFLVQRHFGIGHFHHPWLKQHFAFLYRTILPGFQRSVAIADSNYNWFYGPESQLVFLDSYVMRNGSGNWLAEMIRKNRVVDGPGQAGKGQRWCTLHTEFIWYNPNLTSTAPADFGKPRLHYFEDWGVVTYGSALPAGINRTFLSFKSGKLGGRAIFDIVHRNKYKDWIKGWRNFNAGHEHPDQNSFTFAPNGVPFITEALYGPKYTFLNNVVMFSPAASDSCFAPWEGQVTEACTSKWLKYKQGAAADCQGKVVAALERNGMVFIRGEGKGAYSPGLKIKSFQRNLLLLQPQLLLLVDHVHLESDSPVGLMSSYFHNTDLPFEETVKDGVHGAFIRHRDGLYKMFWMDDTGYSDKGVLGYRNYPRGYPYNGSNYVNVTLPLRNPNSRGVFIFFGPDMDVQSFSIRSDAQRVDIYLATSEHTYTVYLLTGEVTSKPLFAMVLADHEKVVFEKASGVKESSPAEVNNYANFVEDNLQHAKPVFQQLQKQILARVLNTDNFRKTAERLLDFSDQKKTEEIIEKMFTLKKKQNRNKNSKKGALADKLSDGLPDIFAQIEVNEKKERQKAMKRVYDEDIEEADGDARAFLDYSDLRLPKGRKSGIFKQRKYKELPMVSTDRSTDPLISASYIRLFLILNIATFFFLLALLLTRFQKTQGLHTKRCFYGIVLVDSFILLCLYSSCSRAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK80</accession>
    <name>W5NK80_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCN family member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cellular communication network factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Connective tissue growth factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with TSKU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK80"/>
    <dbReference type="FunCoup" id="W5NK80">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021039"/>
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        <property type="protein sequence ID" value="ENSLOCP00000021039.1"/>
        <property type="gene ID" value="ENSLOCG00000017020.1"/>
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    <dbReference type="eggNOG" id="ENOG502QQDX">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155019"/>
    <dbReference type="HOGENOM" id="CLU_063247_1_0_1"/>
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    <dbReference type="OMA" id="ERDPCDH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017020">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:cell adhesion"/>
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    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:endoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0031099">
        <property type="term" value="P:regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Connective tissue growth factor (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCN"/>
    </dbReference>
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        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
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        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
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        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
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        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
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        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF7">
        <property type="entry name" value="CCN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWFC_1"/>
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    </dbReference>
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        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867870" description="CCN family member 2" evidence="16">
        <location>
            <begin position="32"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="19">
        <location>
            <begin position="30"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="18">
        <location>
            <begin position="105"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="17">
        <location>
            <begin position="260"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041320"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042353"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00046496"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="353" mass="39025" checksum="D8D8D894B4B4AE42" modified="2014-04-16" version="1">FTATAMKMSAGMNNLQFTSFLCLALFYWVSAQECSGQCRCPEAPPQCPAGVSLVTDSCGCCRVCAKQLGDLCTEHDVCDPHKALFCDFGSPVNRKIGVCTAKEGSPCVFGGMVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDIRLPSPDCPAPRRVKVPGKCCEEWVCDAPRRQTMVGPALAAYREEDTYGPDPSLMRQNCLVQTTEWSACSKTCGLGISTRVTNDNRECRLEKQSRLCMVRPCESDLEQSIKKGKKCIRTPRVSKPMKFELSGCTTTKTYRPKFCGVCTDGRCCTPHRTSTLPMEFKCPDGQVMKKQMMFIKTCACHYNCPGENDIFQSVYYKRMLGDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLA6</accession>
    <name>W5NLA6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NLA6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021415"/>
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        <property type="protein sequence ID" value="ENSLOCP00000021415.1"/>
        <property type="gene ID" value="ENSLOCG00000017310.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6PI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_077975_8_1_1"/>
    <dbReference type="InParanoid" id="W5NLA6"/>
    <dbReference type="OMA" id="FRQYPNG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017310">
        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002173">
        <property type="entry name" value="TRADV8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="116" mass="12950" checksum="C64260889AB21920" modified="2014-04-16" version="1">ITITYCNFYSFSAAVHCDSISPQQTKVSGEEEGSVTLQCSYSTSNAGYRLYWYRQYPHRPLQYILYKGAGTWSGDHTADFAKDRFRSAVSGSSTTLRITKLSLSDSAVYFCALRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLS4</accession>
    <name>W5NLS4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01022327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLS4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021583"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021620.1">
        <property type="protein sequence ID" value="ENSLOCP00000021583.1"/>
        <property type="gene ID" value="ENSLOCG00000017478.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165350"/>
    <dbReference type="HOGENOM" id="CLU_000384_10_3_1"/>
    <dbReference type="InParanoid" id="W5NLS4"/>
    <dbReference type="OMA" id="DMANAFW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017478">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="5">
        <location>
            <begin position="157"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="612" mass="68707" checksum="1393A610C6E079CC" modified="2014-04-16" version="1">MSLLWDTGAQLSCLSEQSAKQCGVMFTGRELEATGVGGNRITLRETQKLKIRLGDGEVTFNAVMWVGPTPDLLGWDALKYLQGVTLTVHEERVEVNIWSFQKNELKGNSIWTMDKDECGLLDMEPATFTGKSPPYTKPYKINKEAEEGVRPVIQKLLAQGIVRKTHSTSNSPIWPVKKASGAWRFTVDYSKANQHINRLTPLVADPSAIFQLLTPEHQVFSVIDMANAFWSVPLHPEVQDWLAFVDGEQYTWTRLPQGFHNSPTVYHMALVRHLRELPPVSFVVIQYVDDILIVSPDAETHKKDLQIILDHLVKKGHRASYSKAQLCLDTVIYLGQKISPGKRELTEDWAQAIRTHPSPNTVRELRSFLGLCNFNRNWVGAFSERAKPLTDLLKGGGRSLDPIAWSPEVEHAFNDLKQALLQAPALGFPQTDQPFYLYVGEKNGFWTAVLTQEHGGRYRPVGYYSGSLDEVARGWGPCLRAVQAVCLAVQAVNNIVLDQQLIVRCPHAVHTLMSLNRVSQVNAIRWSKWWVLLEAPNITIEREGALNPATLLPDPSEAYMLEHSCDVWAMGEDEGIKIAEQPLRNEDLILFTDGTSFINSSEESGLGCDYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLU4</accession>
    <name>W5NLU4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SLIT and NTRK like family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLU4"/>
    <dbReference type="FunCoup" id="W5NLU4">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021603"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021640.1">
        <property type="protein sequence ID" value="ENSLOCP00000021603.1"/>
        <property type="gene ID" value="ENSLOCG00000017498.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQXQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160971"/>
    <dbReference type="HOGENOM" id="CLU_012706_1_0_1"/>
    <dbReference type="InParanoid" id="W5NLU4"/>
    <dbReference type="OMA" id="EPSMFIH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017498">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000001">
        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF3">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869543" evidence="10">
        <location>
            <begin position="20"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="620"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="11">
        <location>
            <begin position="216"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="11">
        <location>
            <begin position="532"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="838" mass="93955" checksum="0F64241EC500ADF2" modified="2014-04-16" version="1">LKKMLLLVLLAFSVPSSSPNSDSDLSAETCSICSCMSMENVLYVNCEKITVYRPTQLKPPSSSLYHLNFQNNLLIILYPNSFLNFTHAVSLQLGNNKLQNIEGGAFMGLSALKQLHLNNNELKMLRADTFLGIENLEYLQADYNLIKFIERGAFNKLHKLKVLILNDNLIQSLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRIVELQLEDNPWNCTCDLLPLKAWLENMPYNIYIGEAICETPSDLYGRLLKEASKQELCPMGIGSDFDIQMPPLPPENGQTTSNVAPTTIQHLITKAPKTTNPSKISGIIAGKAFPGNRNLTQTVSYQTRVPPFSPCPHPCNCKAHPSDFGISVSCQERNIKNLAELVPKPENAKKLHLSGNNIRDISLSDFQGFEGLDLLHLGSNQIVTVQKGVFANLTNLRRLYLNGNQIERLYPEMFHGLTNLQYLYLEYNAIKEVLAGTFDSMPNLQLLYLNNNVLRSLPAYIFAGVTLARLNLKNNHFMYLPVSGVLDQLTSLSQIDLEGNPWDCTCDLVALKLWLEKLNDGVASKEIKCTSPVQFSNVELKKITNEMLCPKLLMKPPFILTSSTPVITSTSPAGLGKAPPGGPVPLSIMILSILVVLILTVFVAFCLLVFVLRRNKKPAGRHEGLGNQECSSMQLHLRKHDHKSNKKDDLGGETFIPQTIEHMSKSHTCGLKDSESGLKFGDCPMQKIILRNSGEKDKDSLSRDPRKRLSTIDELDEFLPGRESNMFIQNFLENKKDFNSIGVSGFEIRYPEKPQDKKMKKSLIGGNHSKIVVEQRKSEYYELKARLQGSPDYLQVLEEQTALNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMD1</accession>
    <name>W5NMD1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rapunzel 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641837.1">
        <property type="nucleotide sequence ID" value="XM_006641774.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMD1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021827.1">
        <property type="protein sequence ID" value="ENSLOCP00000021790.1"/>
        <property type="gene ID" value="ENSLOCG00000017685.1"/>
    </dbReference>
    <dbReference type="GeneID" id="138216014"/>
    <dbReference type="KEGG" id="loc:102698790"/>
    <dbReference type="eggNOG" id="ENOG502R546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008158"/>
    <dbReference type="HOGENOM" id="CLU_703924_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMD1"/>
    <dbReference type="OMA" id="KPKFREK"/>
    <dbReference type="OrthoDB" id="8549910at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017685">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039051">
        <property type="entry name" value="SE-CTX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472:SF10">
        <property type="entry name" value="RAPUNZEL 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472">
        <property type="entry name" value="RICIN B-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="392" mass="45179" checksum="40B6C5CEDE003CC7" modified="2014-04-16" version="1">MERVEEWVMENKEKIEQGVEIMGKGCEILAATVGQLHPILEAVFVASKEILCNPESKEAKYLTEQFEKVNQRLEGIQDDIESIQRERQKISMNKQNFDLEVQMISQYEKYLEFVSAKPKFKEVRKDKFMNHFQATDGDMNLDALYNAVIGENLSGESVLETVLVTEERSRRAVEEFCAQLKKLFLVGLIAVMGHASLKEGAVGEALMREWGQRMEEVERRMKAAVDDCTNNFAAQARTDMENQVRDKQAKLDQEFVQPLLDFLGKKYDWVFWSVRVFSHSGLWFWNWLAGKKYQGSGGGNFFDVVTKHNVKVVVSYSVGPTPINKSKIQELIERQKMTSNMVDLAQSIYNGVPKCIVHAISRYKDVVESNNFPEDCYYYTKHKKAYLCIHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNB7</accession>
    <name>W5NNB7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNB7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022126"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022167.1">
        <property type="protein sequence ID" value="ENSLOCP00000022126.1"/>
        <property type="gene ID" value="ENSLOCG00000018025.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SJUD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162014"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NNB7"/>
    <dbReference type="OMA" id="WFCDYPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="35127" checksum="A482B56F1ABA21C8" modified="2014-04-16" version="1">SNTTVSQLTEFLLLGGELGHNEFYTELSIILLVVYLLVLMGNLTILTLVVADHRLHTPMYILLSNLSFIDIVITTTVIPKMISVCIKNDMAISISGCFLQMYFYLSLQSVESFLLTVMAYDRYVAICKPLHYNTIVTNGVCALLAVTAWILGLCGASIAVILATQLPFCNNQVFFWFCDYPPVVRLACMDTTFLLDLALGLALVIIYFPFTVIVWSYSKIILSVFRIGSSDGHLKAFSTCSSHLIVVLTFYLAHSCVYISVKFQNVPQRAIMVISIVNCFLTPLVNPIVYSFRNKEIKRALRKHFPRKRTFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNK1</accession>
    <name>W5NNK1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Noggin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216091.1">
        <property type="nucleotide sequence ID" value="XM_015360605.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNK1"/>
    <dbReference type="FunCoup" id="W5NNK1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022210"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022251.1">
        <property type="protein sequence ID" value="ENSLOCP00000022210.1"/>
        <property type="gene ID" value="ENSLOCG00000018109.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696589"/>
    <dbReference type="KEGG" id="loc:102696589"/>
    <dbReference type="CTD" id="30185"/>
    <dbReference type="eggNOG" id="KOG4485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006009"/>
    <dbReference type="HOGENOM" id="CLU_085186_1_0_1"/>
    <dbReference type="InParanoid" id="W5NNK1"/>
    <dbReference type="OMA" id="CMAERSC"/>
    <dbReference type="OrthoDB" id="5950649at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018109">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.520:FF:000002">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.520">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008717">
        <property type="entry name" value="Noggin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN INHIBITOR, NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494:SF4">
        <property type="entry name" value="NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05806">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008129">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3 7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004870038" description="Noggin" evidence="9">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="155"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="161"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR008129-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="24383" checksum="9EDE7160DF4ED143" modified="2014-04-16" version="1">MGFSQGLLVVLMSIHLGASQHFLRLRPSPSDTLPVPDLIEHPDPEYDPREQDLAERTLRKKLGSNFDPNFMSIGAPVLMNLSVQDAQLRVPGPMPNDIKKLDLSETPYGRRVKIGKKARRKFLQWLWTYTHCPVVYTWKDLGVRFWPRYIKEGNCFSERSCSFPEGMFCKPVKSITKTFLRWYCQGFLKQKYCTWIPVQYPVISECKCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV80</accession>
    <name>W5LV80_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LV80"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000037"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000037.1">
        <property type="protein sequence ID" value="ENSLOCP00000000037.1"/>
        <property type="gene ID" value="ENSLOCG00000000033.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="InParanoid" id="W5LV80"/>
    <dbReference type="OMA" id="WLAHITI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000033">
        <property type="expression patterns" value="Expressed in larva and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF127">
        <property type="entry name" value="SERINE PROTEASE 27-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="232" mass="24304" checksum="C05BFD301E8AB98B" modified="2014-04-16" version="1">MASLQRNGSHVCGGTLIASDWVMTAAQCLPSSVNTSEWRVFLGRQNQTSSNTLEKSTGVLNAFTSSLSGTNIALLRLDTNVSFTDYILSVCLAGAGITFTPGTQCWVTGWGNTTAALLQEKSTTIVQCDNGSSADTICTSPLDLEQGDTGGPLICQQDSSWVQAGVVSSRQGCARSSLPDVYTRVSQYKDWINTQVASATPGFVTFSSNGTDPDSGFNCSSLSVTAVAPSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVF7</accession>
    <name>W5LVF7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVF7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000114"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000114.1">
        <property type="protein sequence ID" value="ENSLOCP00000000114.1"/>
        <property type="gene ID" value="ENSLOCG00000000107.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153770"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_1_1"/>
    <dbReference type="InParanoid" id="W5LVF7"/>
    <dbReference type="OMA" id="TIRCKAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000107">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867186" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="12631" checksum="D009672DE9EAC230" modified="2014-04-16" version="1">TMMLLILLLGTLGLFVQESSGQIIMTQSPAAQSVLPGASVSISCQLSSIGRELSWYLQKPGQAPQLLVYGATNHYSGVPDRFTGSGSGTGFTLTISRVQAEDAGDYYCQHDHSFPFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVW1</accession>
    <name>W5LVW1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVW1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000268.1">
        <property type="protein sequence ID" value="ENSLOCP00000000268.1"/>
        <property type="gene ID" value="ENSLOCG00000000244.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R7EM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00420000030545"/>
    <dbReference type="HOGENOM" id="CLU_1664600_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVW1"/>
    <dbReference type="OMA" id="HANFQGY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000244">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000016">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF103">
        <property type="entry name" value="BETA_GAMMA CRYSTALLIN 'GREEK KEY' DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="2"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="174" mass="19705" checksum="6B9CC63A8328CF9E" modified="2014-04-16" version="1">MSKIIVFTKEGFTGRTAEFKNNVHDLEEKGFNNAISSLKVIGVPWVAYYENNFVGKQRVFEEGEYPTLDDKGRFSSLRMITDDLDDPEIQLFEHINYKGREVTLREETNLQAIAFSDIASSHKVKGGVWVLYGGVNRQGSQLVSFPGDNVPNYVKISFNDVASHVRPLLPKNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW93</accession>
    <name>W5LW93_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW93"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000400.1">
        <property type="protein sequence ID" value="ENSLOCP00000000400.1"/>
        <property type="gene ID" value="ENSLOCG00000000366.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153770"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LW93"/>
    <dbReference type="OMA" id="YNIHITT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000366">
        <property type="expression patterns" value="Expressed in mesonephros"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="43"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="150" mass="16503" checksum="CED36BBBDFC7A7CB" modified="2025-10-08" version="2">MVIQSRTGIRKVKYIYNIHITTDPTLADIAPVLPGFVYAISLPESSRQIVLSQSPAQSVLPGSSVMISCTTSSYITSGYSALHWYLQKPGQALQLLVYRATNHYSGVPDRFTGSGSGTDFTLTITGVQAEDAGDYYCQQSYKLHQKKIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXA2</accession>
    <name>W5LXA2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">EGF containing fibulin extracellular matrix protein 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXA2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000759"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000763.1">
        <property type="protein sequence ID" value="ENSLOCP00000000759.1"/>
        <property type="gene ID" value="ENSLOCG00000000683.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159437"/>
    <dbReference type="InParanoid" id="W5LXA2"/>
    <dbReference type="OMA" id="DPLTEHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000683">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048251">
        <property type="term" value="P:elastic fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000201">
        <property type="entry name" value="EGF-containing fibulin-like extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000290">
        <property type="entry name" value="EGF-containing fibulin-like extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000367">
        <property type="entry name" value="EGF-containing fibulin-like extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF26">
        <property type="entry name" value="FIBULIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866955" evidence="12">
        <location>
            <begin position="33"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="177"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="256"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="456" mass="50456" checksum="41FCFD89126DC77C" modified="2014-04-16" version="1">FSECLSLFLACVQTMRPLWVVLLTCALHTAISQEPSESDAFTECTDGYQWDAQTLHCKDINECETIPDACKGEMKCFNHYGGYLCLPRSASVIANNEVNAGAGDPSQSPASLPAAGHTPFNPCSLGYEPQGDTCADVDECEQDLHDCQPSQLCINTAGSFTCQCPDGYRKIGTECVDIDECRYRYCQHRCVNSPGSFSCQCEPGFQLAGNNRSCVDVDECDMGAPCQQRCYNTYGTFACRCEQGYELGEDGFACNDIDECSYSSYLCQYRCVNEPGRFSCVCPEGYQLLGTRLCQDINECETGAHQCSESQTCVNIHGGYQCVETNRCQEPYVQVSDNRCVCPVTKPGCRELPFSIVHRYMSITSDRSVPSDIFQIQATSVYPGAYNTFRIRSGDEQGDFYIRQINNISAMLVLARAVTGPREFVLDLEMISVNPLMSYQTSSALRLAVYVGPYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXD0</accession>
    <name>W5LXD0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATM interactor</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641199.2">
        <property type="nucleotide sequence ID" value="XM_006641136.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXD0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000787"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000791.1">
        <property type="protein sequence ID" value="ENSLOCP00000000787.1"/>
        <property type="gene ID" value="ENSLOCG00000000709.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684821"/>
    <dbReference type="KEGG" id="loc:102684821"/>
    <dbReference type="CTD" id="23300"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013091"/>
    <dbReference type="InParanoid" id="W5LXD0"/>
    <dbReference type="OMA" id="LCALFQH"/>
    <dbReference type="OrthoDB" id="6354171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000709">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055303">
        <property type="entry name" value="ATMIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056545">
        <property type="entry name" value="C2H2_ASCIZ_1st_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056380">
        <property type="entry name" value="Znf_C2H2_ASCIZ_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056381">
        <property type="entry name" value="Znf_C2HC_ASCIZ_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664:SF1">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24757">
        <property type="entry name" value="C2H2_ASCIZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24761">
        <property type="entry name" value="C2H2_ASCIZ_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24759">
        <property type="entry name" value="C2HC_ASCIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="778" mass="85086" checksum="40705E2CED1CDC2A" modified="2014-04-16" version="1">MAASAPGGRAARPSRSSRQEDGEEPSSQPWEMIKPSITELTKEVRTNILCTVEGCGKILPNTPALNMHLVKSHRIKDGLVNPTVRKDMKGSQKRYCCPIEGCPRGPNRPFSQFSLVKQHFMKMHAERKHKCPKCSNCYSTEWDLKRHSEDCGKVYCCTCGCPYASRAALLSHIYRTGHEIPAEHRYPPVKKRKMEGSLSGAMKLRPNEQVFQIVNIEKELSEVFVSANGACQSHSGNQNPKSTINIQKRLLPKPKVALVKVPVMQFTQLPILVSPADSSVKPFVLTVDNQGSLMSTVNLLSPSVGAMIPTLDTKALTFRDTMSASRFSIEPVSTGVQVNLETMASGNVLNYMGSRSKSTSTNIQTDISYLSKVSIPAASCAISESLVSSCSQTDISFSAQVLLPVNVETQTVPSLTKVMSSIGAQTDTSGQACFPSCSINRETQTNAATSTMVDREQVDQAIMCTNLFDNDSLPSFSVSTQTGMKDSRLGMNTVSEHRNSRDSSSFYDEKPGGVMCFSAQTDLLPQNPVADNQTQTMNLFSDLENILSDSLAGHALDSRGGLSDATTSCEAGLTSVQGQNTGIDFDIEEFLNAAHIQTQTEESELGTLSSEPALESLDIETQTDFLFFDNPAPHYGNPRTQSNDIGLEMFDTQTQTDLNFLLDTSSHIPLGNFLKQSSFSMSTESSDTETQTEINSVSQCNTNESQVKLSSTETQTVSSSFECLGNLFLTSNETQTVMDDFLIADMAWNTMESHFSSVETQTCEKLYSLFQHSEKTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ57</accession>
    <name>W5LZ57_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Matrix metallopeptidase 20a (enamelysin)</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ57"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001414"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001420.1">
        <property type="protein sequence ID" value="ENSLOCP00000001414.1"/>
        <property type="gene ID" value="ENSLOCG00000001242.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160903"/>
    <dbReference type="HOGENOM" id="CLU_015489_6_0_1"/>
    <dbReference type="InParanoid" id="W5LZ57"/>
    <dbReference type="OMA" id="RTTYWNR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001242">
        <property type="expression patterns" value="Expressed in zone of skin"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
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    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF297">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016747968" evidence="21">
        <location>
            <begin position="20"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="22">
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            <begin position="106"/>
            <end position="267"/>
        </location>
    </feature>
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            <begin position="333"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="380"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="20">
        <location>
            <begin position="429"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="17">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="159"/>
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        <ligand>
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            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="169"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="171"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
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            <name>Zn(2+)</name>
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        </ligand>
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        </ligand>
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        <ligand>
            <name>Ca(2+)</name>
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            <name>Ca(2+)</name>
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            <position position="367"/>
        </location>
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            <begin position="283"/>
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        </location>
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    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="472" mass="54128" checksum="686FFCAD18C9562D" modified="2014-04-16" version="1">MLPWSSLLFLLGNLTLVVTLPIAAESELKPEDVTFAEEYLSRFYPLREADGTRTRRQAAAAEERLRQMQEFFGLEATGRLSRQTVAVMKRERCGVPDTENYSFYPDKPRWRNSTVTYRIVKYTPDLKRKDVERTFRQALKIWSDAAPLKFVRLDHGEADIMINFGSKAHGDFFPFDGPKGVLAHAFEPGEDIGGDAHFDEDEIWTMGNKKPGYNLFTVAAHEFGHTLGLSHSKDPSALMYPNYKYYNPATYSLPRDDVLGIQELYGFPFQGRKPVALPPPEKCDPYLSFDAVAVIGRDIAFFKNSYMWLRMTWMMNWNSLREGLISSLLPSISSPVDAAYDIPAKGVAYLFTGPKFWIVQQLNMKSYSGSIYDIGFPTDVKQVDAAVHVKEYGKTYFFVGERYYRFDETWSRMDPGFPRRIRTDWPGIGSKIDAAFELAGYIHFFSGPKAYKYDYRQRQVLYAVRANAWLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ82</accession>
    <name>W5LZ82_LEPOC</name>
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        <submittedName>
            <fullName evidence="6">Leucine zipper, putative tumor suppressor family member 3a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023328">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="1032"/>
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        <property type="protein sequence ID" value="ENSLOCP00000001439.1"/>
        <property type="gene ID" value="ENSLOCG00000001268.1"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154078"/>
    <dbReference type="InParanoid" id="W5LZ82"/>
    <dbReference type="OMA" id="NGMAENR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001268">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF6">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 3-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="416"/>
            <end position="452"/>
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    </feature>
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        <location>
            <begin position="87"/>
            <end position="193"/>
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    </feature>
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        <location>
            <begin position="236"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="430"/>
            <end position="439"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="452" mass="50889" checksum="2E0F4E1FB5FB65DF" modified="2025-10-08" version="2">MTSLPTYTGSGSVYGPPHPLGPLSASTSHINRLGTAGAYDKPGCQNGLSASDSGRSSSGKSSSSCQRLSHLSDAPPLRPSPSSDDIIQDLEDRLWEKEQEVLHMRRNLDQSEAAIVQVFEEKQRVWEREMEELRQNYANKLQQVSRKAQRTQQTLQLQISRLQQDKRRLQEELAALLAQREELERKCLDYKKEQADILPRLEETKWEVCQKAGEISLLKQQLRESQAEVAQRAGEMVALRAQLRELSGQLREREEAVLGLKDSYSSKSLALERCEGELQRTLAEVSLLRDKLGVFEAEVLGLKQALVELGGAGRAAVPSPQSPPDGPPPPQADALLSLQSDEAKAQRQAEAGGLRRQLERLQGELRLERQQRERQALSFAKERHTWQGEKERVLKYQAQLQLNYVEMYQKNQALEQQVGQLSSKEDKKPSPPQLTPPWGGPSRLERIESTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZF5</accession>
    <name>W5LZF5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking kinesin protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the milton family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZF5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001512"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001517.1">
        <property type="protein sequence ID" value="ENSLOCP00000001512.1"/>
        <property type="gene ID" value="ENSLOCG00000001329.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4360">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155697"/>
    <dbReference type="HOGENOM" id="CLU_013450_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZF5"/>
    <dbReference type="OMA" id="LRTETCH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001329">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051946">
        <property type="entry name" value="Intracell_Traff-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022154">
        <property type="entry name" value="TRAK1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751:SF11">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04849">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12448">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01423">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAP1 N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking kinesin-binding protein C-terminal" evidence="7">
        <location>
            <begin position="324"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="664"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="807"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="94"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="831" mass="91173" checksum="1C2CECEB3DB701B6" modified="2014-04-16" version="1">ITNLLCPVLVPADVCNSTDLPEVEIISLLEEQLPHYKLRADTIYGYDHDDWLHTPLISPDANIDLTTEQIEETLKYFLLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQSLLKKNKTLTERNEYLEEQVEHIREEVSQLRHELSMKDELLQFYTTAAEESDADSACCTPLRCNTVSTSAFTSLKDGPAVSSSASLRAFFLNCDKKPRGAICSVRPRVSSSLESFFKLLRELEDKYAECMEMLHEAQEELKNLRNKTVPSSTPRRFHALGLFPMDSLAAEIEGTMRKELHMEDPEMEEHKLHHKRVFETVKNINQTAKQRSLLPSPMNIPGSNQSSALSSCRSTPRSSFYGSDVSNIVIDNRTNSIILETESPEGSDGDANKKPGTPGTPGSRDLEVALRRLSLRRENYLSERRFFEEERERKLRDLADGGELHSGSLTPAESIMSLGTHHSGLSVFSFGSRSYLPEKLQIVKPLEGSATLHHWQQLAQPNLGGILDSRPGVVTKGFRPLELDLEEVYQFTDFEEDEPGDLSFQSLSTSSPAPPHAEASVHYPGKCMSHTNSTYTFTTCRILHPSDELTRVTPSLSSGPMPATVCMSSLKSTPVGTPCTPRRLSLAESFTNLRDSTKTTSTSLGLVRLLQERGISAAVYNPQSWDRAATGAGTLAGPPRAPETLPSTPPNSPTTAAATATTTTSEQPQQQPGSSAPFEFKGPGGPYDNFLASKPASSILKEVRAESRSTCSSGSQTDVSVSNLNLLDKLKPFVPAGLAREGLALGCPGVTHAIGGIRRNRSYPAVVGASMQMKGPGPQSSELILGPQLPKQTSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0N0</accession>
    <name>W5M0N0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0N0"/>
    <dbReference type="FunCoup" id="W5M0N0">
        <property type="interactions" value="1743"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001937"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001942.1">
        <property type="protein sequence ID" value="ENSLOCP00000001937.1"/>
        <property type="gene ID" value="ENSLOCG00000001683.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699251"/>
    <dbReference type="KEGG" id="loc:102699251"/>
    <dbReference type="CTD" id="6175"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017839"/>
    <dbReference type="HOGENOM" id="CLU_053173_1_1_1"/>
    <dbReference type="InParanoid" id="W5M0N0"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001683">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="34244" checksum="E9D94FCBCFD2FB3F" modified="2014-04-16" version="1">MPREDRATWKSNYFMKIIQLLDDYPKCFIVGADNVGSKQMQTIRLSLRGKAVVLMGKNTMMRKAIRGHLENNPSLEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCDVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLIIQQVYDNGSVYSPEVLDITEDALQQRFLEGVRNVASVCLQIGYPTVASIPHSIINGYKRVLAVAVETDYSFPLADKVKEFLADPSAFAAATAAPVAAESAAAAPAAVKEEVKEESEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0R9</accession>
    <name>W5M0R9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Collagen type VI alpha 6 chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001976"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001981.1">
        <property type="protein sequence ID" value="ENSLOCP00000001976.1"/>
        <property type="gene ID" value="ENSLOCG00000001668.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155619"/>
    <dbReference type="InParanoid" id="W5M0R9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001668">
        <property type="expression patterns" value="Expressed in larva and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005589">
        <property type="term" value="C:collagen type VI trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22630">
        <property type="entry name" value="Kunitz_collagen_alpha6_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000016">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000118">
        <property type="entry name" value="Collagen type VI alpha 6 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000021">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF86">
        <property type="entry name" value="COLLAGEN, TYPE VI, ALPHA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865386" description="Collagen type VI alpha 6 chain" evidence="15">
        <location>
            <begin position="24"/>
            <end position="3022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1294"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1331"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="35"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="238"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="443"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="633"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="840"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="1034"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="1369"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="1571"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="2322"/>
            <end position="2465"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="16">
        <location>
            <begin position="2531"/>
            <end position="2732"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="17">
        <location>
            <begin position="2972"/>
            <end position="3022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1971"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2788"/>
            <end position="2815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2029"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2124"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2190"/>
            <end position="2200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2788"/>
            <end position="2801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3022" mass="333091" checksum="660D9A60C22CB293" modified="2014-04-16" version="1">SSMEMIQFLSAFLFAFCLPVNNAQKTVCTEETAADIVFLVDGSWSIGTENFQQIRDFLYALIDSFDVGADKVQIGLIQYSNSPRTEFTLNQYQNKQDILTYIQSLPYKGGGTRTGLGLSYMLDNHFTEAAGSRVREGVPQVAIVITDGQSQDTVKQAAEEVKNSGITLYAIGIKEAVLEELNEIASDPDDKFVYNVDDFAALQGISKNFVQVLCTTVEESKRQTLQVSKECRQATAADIVFLVDGSTSIGETNFNEVRNFLYTFVEGLDVGLNKVRVGLAQYSDEVYQEFLLNKYSDKQDILEQMQNLQYRTGGTNTGKALEFLRTQYFTEEAGSRATLGIPQVVIVITDGVSADEVREPAKKLRENVTVFVVGIGVAAYDELQEIANRPSDKFLFNIDNFEALNQLSSSLLPNVCTSIGTQKEENQLTPFLVVVIQKPMVLYIVFLVGGSTLLGSPAFQQIRSFISRIVNQLDVGINQHRVGMAQFSGDTKTEFLLNTYEKRGEVLNHLKNNFRLKGGAQRLGQALNYVHRTFYKESAGSRIAEGFRQFLIVFTSAKSEDEIQRAARIIKAEGVTVISIGLPRAPITELEVVATKPYVYQLNSQVFSKTVQEVSGIIESKEAHPCKSATVADIVFIVDESASIGTQNFRSVRNFVYKIIDGLDVDLNKVRTGVIMYSDAPRAEVYLNSFREKAEVLRYIKTLPYRGGGTNTGAALDFARQNMFVKGRGSRRSQGVQQIAIVITDGESQDNVSGPAVSLQRAGVTVFALGIKEANMKQLKQIASYPSRKFVFNVDSFAKLASVEKSLQKLLCSEIINIAFAAPRVIYSLKEGCVETEEADIYFLIDHSGSIDPLGFVDMKKFIKEMIRMFRIGPQSVRIGVVKYANTPTVEFTVTEHTNKKDLERAVERVQQLGGGTQTGDALRSMSALFQKAASSRNRKTPQFLIVITDGKSQDAVVDAAKELRAQSIMIYAIGVGDANEDELLEMSGSPDKKFYVSNFDSLRLIKNEVAQELCSEEAFFSFFLLVCKTMEADIIFLIDSSGSIHPDDYSKMKTFMESMVDRSDIGADKVQVGVLQFSSVQQEEFPLNKYEDKSGIKQAIYNIQQLGGGTLTGEALKFTSQYFDAARGGRSTVKQFLIVITDGEAQDEVAKPAQNLRNKGIIIHTIGVMNANDTQLVEISGSQDKVHSAKNFDGLQFLEKNILFQICNPDTGEESGVTLQQNIVESNMLKCHCRVHPKSGSMRVLLLEMPEQFQTNLNFSRLHQYLQNIAKHITGRTEHLLNLPLTVLSQIQYPLYCIIFNALLVSICAYFCFTPIYFEFISLTNKVFKLLTFLLHIISCYYTVPIIIHFPLYLITFLYIECRTEVADMIFLVDESTTIDSSEFVSMQKFMIAMVNNSDVGKNRVRFGAIKYSTTPTEMFRLNQFDSKQQVRDAIAAMTADGGDTYTAKALQFSQTFFTEAYGGRKSAGVPQILLVITDGEATDRYELPKSSMRVREDGISIYGIGVENATEEELKIMTEDETKVFYVNNFKGLEELQKNITKKLCNDTKPGRQACLLDYTYSCETKEADIVMLIDGSGSINPGQFQTMQNFMKDIVGSFRISKTSVQVGVAQFSTEPQKEFYLSEFDSEDAIKERILQMKQMKQSTYIGKALRFIRSFFEPSAGSRIRQFVPQNLIVITDGKSIDPVEEAAAELRALNIHIFVISIGYVDALKLQQIAGSNDRLFTVKNFNELETIKKRVVKNICDPGDEPSTSNCTIDIAVGFDISRRGRFPGLLSSQRKLEAQLLGLIRQMSYLDNLNCVSASQVIIQIGFLISENGRIIFDSNFEKYNEEIVRKVMVTLQTMEATYFTTKLLQSFLDKFKGKSTANVKVLLVFSDGLDENVELLETESENLRRGGINALLTVALEGVTNANDLQMVEFGRGFGYKVPLAIDMHNINSAMAKELDTIAERVCCNVMCKCMGQEGLRGPRGPLGIKGSPGTKGPPGHPGDEGGFGERGTPGLNGTQGIEGCPGKRGIKGPRGYRGNRGEDGDHGIDGVDGEQGMAGLPGALGERGDPGSPGRSGVRGEPGERGQPGLRGDPGDSGTDNNIRGPKGEKGNPGIQVAPQGDAGEDGTPGDAGVDGKRGERGEPGTPGLRGDPGASGPQGERGTRGLPGPPGTLGLPGRQGELGLQGPKGSVGNEGPKGQKGQPGDPGVKGSVGPNGPRGLPGIDGRDGYGSPGQKGEKGESGFPGYPGPQGEEGDLGTPGSRGPKGSRGRRGNSGSSGVPGDPGIPGASGHKVTVVPQFSLFHYFVGDKYWHKSNCFYRIHMTDCPVYPTELVVALDMSEDVTPQVFERMRNIAVNLLQDLTISESNCPTGARVAVVSYSSTTKYLIRFSDYHRKKQLIEAIKNIPPKRTTSRRNIGAAMRFVARNVFKRVRQGVLMRKVAIFISNGASQEATPIVTAALEFKALDITPVVIAFRNVPNVRRAFQVDETGSFLVSVVDRPQNQRTEVQRIQQCALCLDVCNPSEACRINLIPAPLQADVDLALVVDSSRNVPTDQYDGVKELLSSILDQLDVSSQPGTSNRGARVALVQHSTLNYPPRRGQAPVRTEFDLLKFKSRNLMKRHISESMQQLGGSSSTGHAVEWAINNLLLKAVRPRKAKAVFAFVGGETSYWDRARLGYVARRAMCQGVAVLAFSVGSDYNDTQVEDLASIPLEQHMVHLGQAKLGEIEFAQRFARAFFRVLSTGVNSYPPPALRRECANIQEPVQQAEILETPPIDRIKPKTPPPAEEYYEYTEESESIVEEESEPEEEITAGPELSQEETVSQYEETGRAVEEILFGGENYLCNTVSQRQNISDCVNVLCVVLHVNSQTNACWMWISVAFVGTICKDGIIIGQSVHAPLSGMAVVAVMRIDLIRNKSHTIQTLAETLQCKNIRHFSKPQVPSLTPLYRQDLRLCLKIYFCHKVTLCTVQASPQFFSTDACTLPKDEGDCRNFTLKWFFDGQQNECSRFWYGGCGGNRNKFETQEECEAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0T9</accession>
    <name>W5M0T9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627791.1">
        <property type="nucleotide sequence ID" value="XM_006627728.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0T9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001996"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002001.1">
        <property type="protein sequence ID" value="ENSLOCP00000001996.1"/>
        <property type="gene ID" value="ENSLOCG00000001730.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693875"/>
    <dbReference type="KEGG" id="loc:102693875"/>
    <dbReference type="eggNOG" id="KOG3885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160956"/>
    <dbReference type="HOGENOM" id="CLU_081609_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0T9"/>
    <dbReference type="OMA" id="FFWIYLS"/>
    <dbReference type="OrthoDB" id="6158176at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001730">
        <property type="expression patterns" value="Expressed in heart and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="203" mass="22888" checksum="2965FBE2FE918AB3" modified="2014-04-16" version="1">MAPLGEVGNYFGAQDALPLGNVPVLPADNPHLVHSEAGGLPRGAAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRQDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTPECVFREQFEENWYNTYSSNLYKHTDTGRRYYVALNKDGTPREGTRTKRHQRFTHFLPRPVDPDKVPELYKDILSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1C4</accession>
    <name>W5M1C4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UEV and lactate/malate dehyrogenase domains</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193802.1">
        <property type="nucleotide sequence ID" value="XM_015338316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1C4"/>
    <dbReference type="FunCoup" id="W5M1C4">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002181"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002187.1">
        <property type="protein sequence ID" value="ENSLOCP00000002181.1"/>
        <property type="gene ID" value="ENSLOCG00000001881.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684430"/>
    <dbReference type="KEGG" id="loc:102684430"/>
    <dbReference type="CTD" id="55293"/>
    <dbReference type="eggNOG" id="KOG1495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153903"/>
    <dbReference type="HOGENOM" id="CLU_039842_0_1_1"/>
    <dbReference type="InParanoid" id="W5M1C4"/>
    <dbReference type="OMA" id="CIMGANG"/>
    <dbReference type="OrthoDB" id="5405561at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001881">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042867">
        <property type="term" value="P:pyruvate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF33">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UEV" evidence="2">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="483" mass="52279" checksum="F081D9396CF3372E" modified="2014-04-16" version="1">MDLSQESIRKTLGKYKFRDVAIEELQKVHRVNPGFKPLVDTYTFSDSSQKDLLKLVGNIPVSYQGRSYNLPILLWLLDSFPFTPPICLLRPTPSMMIRVGKHVDARGRIYLPYLHNWDHPASSVNGLLSEMIAKFEEEPPLCTKPSADEKDPAELLAYVEKISGGMSTMNIGPPASVGRVYPERPVNKVTVVGGGDLGVACLMSILAKGSVDKIVLIDFSEHSSKGGTMDLEIFSLPKVEVARDLSASADSKVVVVTSNAWSDDESFVSVVQTNVDLYQGIIPCLARCSPSAVLLIASQPVDIMTHVAWKQSGLPPSRVIGTGCNLDSERLNYIVSTVLLSRAAGSQPWVVGELSDSKVPAWSSSAPATDSQPEITPGPTSTKPLTDRAFEILKGRGQRSLSVGLSVADIVHSILADQRKTHSVSTLAQGWGGVCGEVFLSLPCELGTGGVARISKLTLGQEEDLKLRNSATSLRNFIQQLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2T9</accession>
    <name>W5M2T9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal kinase-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">SCY1-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2T9"/>
    <dbReference type="FunCoup" id="W5M2T9">
        <property type="interactions" value="1701"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002697"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002703.1">
        <property type="protein sequence ID" value="ENSLOCP00000002697.1"/>
        <property type="gene ID" value="ENSLOCG00000002300.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151054"/>
    <dbReference type="HOGENOM" id="CLU_010392_0_1_1"/>
    <dbReference type="InParanoid" id="W5M2T9"/>
    <dbReference type="OMA" id="EFVVSWG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002300">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000108">
        <property type="entry name" value="N-terminal kinase-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="7">
        <location>
            <begin position="390"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="589"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="599"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="778"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="877" mass="95451" checksum="3F492619DA9D0770" modified="2014-04-16" version="1">MWSFFARDPVKDFQYEILPDTQEPSGIWTLHRGKRKLWGEILQVGLGRRGKKTKRGGQNAYSHELSMTFKNNHLNVRLCPLPYPGDLPTPRFLKRSLFPLGPIAAGSELVCHTGPGPTCSSSWCISGLWGGDWTHKNSKTVSFSFVCAKETGGNGLKKKTTAPRESGSALPPPPPVSRGPPHTESVTPASRGQSGGEDWCRRSADVWRLGCLIWEVFNGPLPRASSLRSLGKIPKQLVPHYCELVGANPKARPNPARFLQNCRGPGGFMSNSFVETNLFLEEIQIKEPAEKQKFFQELSDNLDSFPEDFCRHKVLPQLLTAFEFGNAGAVILTPLFKVGKFLSAEEYQQKIIPVIVKMFSSTDRAMRIRLLQQMENFIQYLNEAAVNSQIFPHVVHGFTDTNPAIREQTVKSMLLLAPKLSETNLNAELMRHFARLQARDDQGPIRCNTTVCLGKIAPYLNATTRQRVLISAFSRATKDPFPASRAAGVLGLAATHSFYAASECACRILPVLCALTVDPDKSVRDQAFKAIKSFLSKLETVSEDPSKLAEIEKDVHSSASSPGAAAASWAGWAVTGVSSLTSKLIRTAPDHAPPATAAPAPPAGPPPNGTSGSRVEEAKKEAPRPQPASSGLADQWAAAKEQEEEEEPASDRWEEEDWGSLEEQDPEPLKVQVDPDDWNSSDWSAVSSVTKKANEKGVAGTSSTSSAVKKQSSDWSSSGWEADDSWSNEKEADGQGQSSPGDEGWGQDWEEDPGSRREAAPLALPEGTRLASEYNWDSGGGNGGGGGGKGSQNDFFLGISQRAGPTSTKTGDPWGADTAGDWGAEENWESLEAEQGLTKAELAKKKKREDRRKELEAKRAERKGAKGPLKLGARKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3F1</accession>
    <name>W5M3F1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein disulfide-isomerase A5</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01011607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3F1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002909"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002915.1">
        <property type="protein sequence ID" value="ENSLOCP00000002909.1"/>
        <property type="gene ID" value="ENSLOCG00000002474.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156797"/>
    <dbReference type="HOGENOM" id="CLU_021181_1_0_1"/>
    <dbReference type="InParanoid" id="W5M3F1"/>
    <dbReference type="OMA" id="FCKKMKP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002474">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02997">
        <property type="entry name" value="PDI_a_PDIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd03067">
        <property type="entry name" value="PDI_b_PDIR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000029">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000105">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046374">
        <property type="entry name" value="PDI_a_PDIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041865">
        <property type="entry name" value="PDI_b_PDIR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF2">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865491" description="Protein disulfide-isomerase A5" evidence="11">
        <location>
            <begin position="32"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="142"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="270"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="387"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="521" mass="59611" checksum="6D442E97D29CBED3" modified="2014-04-16" version="1">MATWNLCGSGMRGVKMCMFVYLSILPALLQAAKVSPLIEKVSDHKDFKKLLRTRTNVLILYTNSASSGESQLKLLSNVAQAVKGEGTIAWVNCGDSESRKLCKKMKVDPSSKRSGAEMIHYKDGTFHTEYNRPETFKSMVAFLRDPTGPPLWEENPEAKDVVHIENEKDFRKLLKKEERPILMMFYAPWCGVCKRMMPAFQQAATETKGRYVLAGMNVHPAEFDGLKQEFDVKGYPTFCYFEKGKFLHHYENYGATAKDIADWMKNPQPPQPKTPEIPWSEQGSAVHHLTEETFDHFLEEHPSVLVMFYAPWCGHCKKMKPEYDEAAEVLNKDKDSPGVLAAVDATIHKTIGERYHISGFPTVKYFENGEEKYTLPHLRNKDKIIEWLHNPQAPPPPEQSWEEKPSSVSHMGTEDFREALKKKKHALVMFYAPWCPHCKSAVPHFTAAAEVFKEDRKVAYAAVDCTKGQNHDLCKQEGVEGYPTFNYYNYGKYAEKYTGERGEEGFVRFMKTLRGKDQEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4P4</accession>
    <name>W5M4P4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Syntaxin 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01038741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630262.1">
        <property type="nucleotide sequence ID" value="XM_006630199.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4P4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003352"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003359.1">
        <property type="protein sequence ID" value="ENSLOCP00000003352.1"/>
        <property type="gene ID" value="ENSLOCG00000002833.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698613"/>
    <dbReference type="KEGG" id="loc:102698613"/>
    <dbReference type="CTD" id="6810"/>
    <dbReference type="eggNOG" id="KOG0810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234627"/>
    <dbReference type="HOGENOM" id="CLU_042423_2_2_1"/>
    <dbReference type="InParanoid" id="W5M4P4"/>
    <dbReference type="OMA" id="WIAICCA"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002833">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010996">
        <property type="term" value="P:response to auditory stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000022">
        <property type="entry name" value="Syntaxin 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000011">
        <property type="entry name" value="Syntaxin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF97">
        <property type="entry name" value="SYNTAXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="203"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="300" mass="34384" checksum="DF820E07FCB77FE3" modified="2014-04-16" version="1">MRDRTRELGDKAESSDEEEEGRPLMIKPGTGKTSQKAAKDENDEFFQKVREIHEGLAALTMKVNSLEVKQRTILGVALPEESMKRDLQALRDEIKMSASHIQKKLKSIEPKKEEEDGKYVPVNVRMQRTQHGILSREFVELMGRCNAVQSQYRDRNVERIQRQLKITGTNVTDEELDQMLESGQTDVFTQNILRDTHATKQALNEIETRHEEILKLERSIRDLHEMFQYLAMEVEAQGEMVNRIESNILQSSNYVEKATADTEKAKEYHVKSQKKKLCIAVCVIVLILVIIAILAGTFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M571</accession>
    <name>W5M571_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cyclin-dependent kinase 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cell division protein kinase 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cyclin-dependent kinase that phosphorylates the transcription factor ETS2 (in vitro) and positively controls its proteasomal degradation (in cells). Involved in the regulation of actin cytoskeleton organization through the phosphorylation of actin dynamics regulators such as PKN2. Is a negative regulator of ciliogenesis through phosphorylation of PKN2 and promotion of RhoA signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer with CCNQ, the interaction is required for kinase activity. Interacts with ETS2. Interacts with PRK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M571"/>
    <dbReference type="FunCoup" id="W5M571">
        <property type="interactions" value="1485"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003529"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003536.1">
        <property type="protein sequence ID" value="ENSLOCP00000003529.1"/>
        <property type="gene ID" value="ENSLOCG00000002991.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158102"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="W5M571"/>
    <dbReference type="OMA" id="WVARATN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002991">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035173">
        <property type="term" value="F:histone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07845">
        <property type="entry name" value="STKc_CDK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000289">
        <property type="entry name" value="cyclin-dependent kinase 10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000256">
        <property type="entry name" value="cyclin-dependent kinase 10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044093">
        <property type="entry name" value="STKc_CDK10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="50"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="41992" checksum="FCD476E9366BCC23" modified="2014-04-16" version="1">KLGLGCWAVASMDFSEAEPDAIKLRSIKNEKTFTVPQQDRLGNCRSVKEFEKLNRIGEGTYGIVYRARDTKNHEIVALKKVRMDKEKDGIPISSLREITLLLKLRHPNIVELKEVVVGNHLESIFLVMGYCEQDLASLLENMQSPFSEAQVKCIVLQLLRGLLYLHENFIVHRDLKVSNLLMTDKGCVKIADFGLARAYGVPLKPMTPKVVTLWYRAPELLLGTKTQTTAIDMWAVGCILAELLAHKPLLPGSSEIQQIDLIVQLLGTPNENIWPGFSRLPLVGQYTLRKQPYNNLKNKFTWLSEAGLRLLNFLFMYNPKRRATAKDCLDSSYFKEKPLPCEPDLMPTFPHHRNKRAAPVAEGQSKRLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5B6</accession>
    <name>W5M5B6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dipeptidyl peptidase like 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5B6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003574"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003581.1">
        <property type="protein sequence ID" value="ENSLOCP00000003574.1"/>
        <property type="gene ID" value="ENSLOCG00000002992.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154657"/>
    <dbReference type="HOGENOM" id="CLU_006105_4_1_1"/>
    <dbReference type="InParanoid" id="W5M5B6"/>
    <dbReference type="OMA" id="YTSTEHH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002992">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF21">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="5">
        <location>
            <begin position="133"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="4">
        <location>
            <begin position="584"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="797" mass="90655" checksum="D4D7FD119459B28C" modified="2014-04-16" version="1">CLLYVCFFRKLWNAWINLTEDLGGGSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDDTSSGSETKLSVEDLFKPDFAVHDPEAKWISDTEVVYRDRDGHVIKLDAATNDNMTLLESSTFVTFKAAKYSVSPDLRFVLLAYDVKQVYRHSYTASYSIYNILSREVLELNPPEVSDSVLQHAAWGVQGQQLIYIFENNIYYQSDVKSNSLRLTLSGKEGVIFNGIADWLYEEEILKSHIAHWWSPDGERLAFLMINDSLVSNMALPHFTGSLYPRGKQYPYPKKQAGQTNPTVKLYVVNLYGPTHTQELKPPDSFKAREYYVVMMKWISSTKTAVRWLNRLQNISILTVCDTTVGACLEKHEERSEVWLSKQNEEPVFSKDGSKFFLTVPVKQGGRGEFHHIAMFTEQSKTGKTVRHLTSGNWEVTKILAYDENNQTLYFLSTEASTMWRHLYSVSTTDLFPRRCLTCEFSKTNCTFVSADISPNNQYFLLHCKGHGIPTVTVHPLNNPKNNYILEDNTLLRRVVKEKRIQKTEFKAVSIDGFELPLKVSYPVDFSESKQYGLLLIVDGAPGSQSVNDKFLLDWDSVLVSSDGVIVARFDGRGSGFQGQKILQQVHQRLGLVESEDQLAALGYFLKLPYIDPNRIGIFGKGYGGFITLMMLKSSEKQFKCAVAMSPMTDWKLYASAFSERYIGMPSKEESKYQASSVLRNIQGLKEQNLMLIHGTADANIHFQHTAELIKHLIRAGANYTMQIYPDEGHFVSEKSRQHLYRTLITLFRECLKEDTLLLPEEPEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5Q8</accession>
    <name>W5M5Q8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Organic solute carrier partner 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5Q8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003723.1">
        <property type="protein sequence ID" value="ENSLOCP00000003716.1"/>
        <property type="gene ID" value="ENSLOCG00000003127.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686470"/>
    <dbReference type="KEGG" id="loc:102686470"/>
    <dbReference type="CTD" id="100334608"/>
    <dbReference type="GeneTree" id="ENSGT00390000004808"/>
    <dbReference type="HOGENOM" id="CLU_039360_1_0_1"/>
    <dbReference type="OrthoDB" id="2157380at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003127">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="42824" checksum="D64D0F1E52FBFE0F" modified="2014-04-16" version="1">MSMRTLPLLFINLGGEMLYILDQRLRAQNIPADKAKKVMNDIITTMFNKKFMEELFKPQELYSKKALRTVFDRLAHASIMRLNQASMDKLYDLMTMAFKYQVLLCPRPKDILLVSFNHMDAIKEFIRDSPSILNQVDETFRQLIEMYSCLPAGEFQLVRQTLLIFFQDMHIRVSIFLKDKVQNSNGRFVLPTSGPVPWGTELPGLIRIFNGSGEEVKSLEFPSGGNYTSPLREGSFDLYGERVIKLGTNMYSVSRPVETHMSGTSKSSVSHTKENAAPNPLAKEELNLLARLMGGLEVKKPANTDGGFRVNLFTTDEEEEEALISRPPELSYQVINIQATKDQHANAELARIMGEFTEEPGPAQSPGSKGDNLLAMMDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M626</accession>
    <name>W5M626_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Centrosomal AT-AC splicing factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M626"/>
    <dbReference type="FunCoup" id="W5M626">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003834"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003841.1">
        <property type="protein sequence ID" value="ENSLOCP00000003834.1"/>
        <property type="gene ID" value="ENSLOCG00000003234.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS8B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007799"/>
    <dbReference type="HOGENOM" id="CLU_041370_1_0_1"/>
    <dbReference type="InParanoid" id="W5M626"/>
    <dbReference type="OMA" id="MIQDEYT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003234">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028015">
        <property type="entry name" value="CCDC84-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31198:SF1">
        <property type="entry name" value="CENTROSOMAL AT-AC SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31198">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14968">
        <property type="entry name" value="CCDC84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="348" mass="39760" checksum="8AEE15BA2A16B7D3" modified="2014-04-16" version="1">MGAFYCSVCKQTSFAGKGHMYAKTHQNKLKAVLSKFLDKVKEARRCLKSPRVEKFDPTEHERHVWCYCCGQEVRRHVTNGNLTVLHGGLLEHLASQEHRKSTNRFWWENKADLKLKEKFLVTEDETDRCRRQRFKAEVAKALEAFEEKEDEYIKQQAAHIQAVEKQRQEILQSLLEPETEREIPNGLTQPDSWNDEASAGSSSHCGTWRPAELTSPQETGPVEDTVWAGTALSLTFIGHQDASNGGNIHTGATPPWLQDDPQEEGSRGGGREIGPSHEEFLKRKEQEKLRKLPAHRVGANFDHSSQTDAGWLPSFGRVWNSGRRWQSRHQFRAEEGELAQRKRARGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M713</accession>
    <name>W5M713_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 13">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding. The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp. Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity. Interacts with CHODL. Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 13"/>
    <dbReference type="EMBL" id="AHAT01018239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M713"/>
    <dbReference type="FunCoup" id="W5M713">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004171"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004179.1">
        <property type="protein sequence ID" value="ENSLOCP00000004171.1"/>
        <property type="gene ID" value="ENSLOCG00000003528.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183128"/>
    <dbReference type="HOGENOM" id="CLU_028946_3_0_1"/>
    <dbReference type="InParanoid" id="W5M713"/>
    <dbReference type="OMA" id="VKRCQCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003528">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000004">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 13" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="14">
        <location>
            <begin position="25"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="338" mass="37559" checksum="DA5D0207006E9BFD" modified="2014-04-16" version="1">KRLSFSVGGTQVKDVVIKPDAPSVLLLDKHADYIAAYGTKKDDYEYTLSEYLRMSGIYWGLTVMDLMGQLHRMNEDEIIEFVKQCQHESGGISASIGHDPHLLYTLSAVQILSLYDSLSVIDVDKVVEYVKGLQQEDGSFAGDKWGEIDTRFSFCAVATLALLGKLDTINVDKAVEFVLFCMNFDGGFGCRPGSESHAGQIYCCTGFLSITGQLHRVNADLLGWWLCERQLPSGGLNGRPEKLPDVCYSWWVLASLKIIGRIHWIDKNKLRSFILACQDEETGGFADRPGDMVDPFHTLFGIAGLSLLGDEQIKPVNPVFCMPEEVLQRIGVHPEILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M722</accession>
    <name>W5M722_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon regulatory factor 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M722"/>
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    <sequence length="453" mass="51849" checksum="A0065AA90A2D1AE2" modified="2014-04-16" version="1">MEDNCRNMRLREWLITQIDSGKYAGLSWENQEKTMFRIPWKHAAKQDYNQNEDAALFKAWAVYKGKYREGKDKVDPSTWKTRLRCALNKSTDFQEVPERSQLDISEPYKVYEILPEGGRRAESPSSPVIFGTGRTASPHIVTPSLHSQVIADPSFYCSIFSAPMEVEQEWRERTDADSLHYQPASSVESHSLVQSATPDSAKQVKGKFYSWTMTAVEGSETPITLQPTVCPTYSPQFQISDFLLQVRLYYQGQLVQDFTTSTPEGCRILHGSVPVENERIYGPTAVELLHFPSPQCAQASLRVTEAMARLLPHLERGVLVWVAPDGVFIKRFCQGRVYWDGPLAQHHNLPNKLEREKTCKLLDMQVFLQEVQQYLRVGRPVPHYQIELCFGEEFPDPNQSKAKKLIMAQIEPLFVRKLLMNIQRTGVEAGQRIQPLKSKSDNIQQTVLKQVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8D7</accession>
    <name>W5M8D7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        </reaction>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000003884.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Linkage group LG13"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="89"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="841"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="841"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="919"/>
            <end position="928"/>
        </location>
    </feature>
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        <location>
            <begin position="929"/>
            <end position="961"/>
        </location>
    </feature>
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        <location>
            <begin position="1000"/>
            <end position="1015"/>
        </location>
    </feature>
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        <location>
            <begin position="1023"/>
            <end position="1037"/>
        </location>
    </feature>
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        <location>
            <begin position="1043"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1061"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1091"/>
            <end position="1102"/>
        </location>
    </feature>
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        <location>
            <begin position="1141"/>
            <end position="1160"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <end position="1192"/>
        </location>
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            <begin position="1207"/>
            <end position="1222"/>
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        <location>
            <begin position="1250"/>
            <end position="1278"/>
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    </feature>
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        <location>
            <begin position="1286"/>
            <end position="1300"/>
        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1308" mass="143329" checksum="3992EED044ACD137" modified="2014-04-16" version="1">MSKAATKEKKSFSKKLFRRGSVRSVGSFMSRVLKTLSTLSHFGTDEHPVEDEKDDGGFATFKSGGKSSTQSDESDCGGFLIGDRVPGVAGLKNHGNTCFMNAVLQCLSNTELFAEYLALEQYRGDEDADDQPKANSVLLQKKGPQERGEVTEQLSGLVRALWTFEYTPQHSRDFKNVVSKNALQYRGNAQHDAQEFLLWLLDRVHEDLNNIVNPSTRSSIKPPLEDDDAAPEGPSLPLAGSFIQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKCSHCMRIGVAVPLSSTVSRLREAVSRETKIPTDQIVLTEMYYDGFHRSFCDDDDDLEIIQESDSIFAFETPEIFRPETILSQRGLHGNLNQNNLKFGTDNSRTSSFMQGAVKPVLPSNKNASSDKIILLVCNRACTGHQGRRFGHPFVLYLERTVTWDVLQKEILEKMRHLLRPGVYIQVGPFSLRVVGVVGITYLLPQEEQPLCHPTVERAYKSCGQGGPPHIKIVVEWDKETKDYLFGHTEDEYIPDIGPVLVWVSAHFPDTCWTKLVVGSFPGALQLAPDDAWRCPHCKQLQQGSIKLSLWTLPDVLIIHLKRFRQEGDRRMKMQNMVKFPLMGLDMTPHVVKRSQSSWSLPSHWSPWRRPYGLGRDPEDYLYDLYAVCNHHGTMHGGHYTAYCKNSVDGQWYCFDDSDVQPISEDEVCKQTAYILFYQRRTTIPSWSANSSVAGSTSSSLCEHWVNRLSGSKQPSVASAASSRRTSLASLSESVEFTGDRSEDDGGFSTRPFVRSVQRQSLSSRSSIASPLAVIENGIKPSWSLSAKLQRRSNSPSHFSLDSHSSPTLERIGEASDDKVSTSCFGSYSKHEHKPSGKAPLAVMEGVTSDENGHKRSVVSLEEPYCKIPGQTEKRSSKAESTDNNNQISSADQGGFGCSSSTKDVKNRSGSGASAQKLESMATKRSSTKAVLEPEKSSKKRQTISSAHEPPASQVTSSPQVKSSSKASSSKEKVSSTKPGSSKMSSSTTQPKKEPSQAQEGTLSGAAQQKQRISSTPQSSTSPSPTGRKTSTGIERSSSSGKSKLAEKSVSRESTRTSPLTDKPAGSAPSRTGTPARSPLPKTSEGGRVEGRTVRSSSSSSSVTSLRSPSVSSKMDLRRNSKSEDKGLSFFKSALRQKETRRSADFGKTTMLAKKASEGTLRSGSGNKGSDTGPTEGTGKPLRQVSPEPKTSKTAVKDKESSKTSTPVKRSILSTGKSKSSTSDIGAQSPANGKKPLEKTSSSRKLSSSMQKSARSTQKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8U3</accession>
    <name>W5M8U3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="5">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01011471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8U3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004802"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004810.1">
        <property type="protein sequence ID" value="ENSLOCP00000004802.1"/>
        <property type="gene ID" value="ENSLOCG00000004019.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158407"/>
    <dbReference type="HOGENOM" id="CLU_002738_0_1_1"/>
    <dbReference type="InParanoid" id="W5M8U3"/>
    <dbReference type="OMA" id="CIQTLGT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004019">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032228">
        <property type="term" value="P:regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13364">
        <property type="entry name" value="PH_PLC_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08558">
        <property type="entry name" value="PI-PLCc_eukaryota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000025">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000017">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF102">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE C-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="8">
        <location>
            <begin position="461"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="548"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="884" mass="97507" checksum="10F6C7C47E41076A" modified="2014-04-16" version="1">LPCSPLTPDPRPQDPQAQKAAGSRKKTVSFSSMPSEKKVSSAADCLAFMQCGCELKKVRPNARLYSRFYTLDPDLRCLRWEPSKKEGDRARLDIASIREVRTGKSTDTFRHHGPAEQLADEAAFSIIHGDGYQSLDLAALSADVANIWVTGLRFLLSHPGPGGGEALEGSPGSVMRAAWLAEEFRRADEDGFGIVPEDTAVGTVCRLCPGVKETRVRRKFKEIQCQKEKLTSRVTLEEFQEAFCELCTRPDVYFLLAQLSKERESLDAQDLRLFLETEQGLALAMPETCLDIIRRHEPSAQGRERGLLGVDGFTRFLLSPECQLFDPEHRRVCQDMGLPLSHYYISTSHRSHLVGGQAQGALDLAGLVRALKLGCRCLELAASDGAGGEPVLGDRLHQQQHQRAGASVAPVGLRSALEVVNKYAFLSSPFPLLLYLRQRCSPGQQRALARLLREAFGGRRSLGEGVARRLASDTPGELVAYTKRHLSRVRPSPARLDSSNPNPQDFWKCGCQLVAMNFQTAGAMMDLHAGRFAPNGGCGYVLRPAVMRDEVSYFSANSQGCVPGVPPQTLRVKVISGHSFPKPQGAGAKGEVIDPYVVLELHGIPADCAEQRTRTAAQNQDDPLFDETFEFTVNLPELALLRFVVLDDDYIGDDFIGQYTIAFECLQPGYRTVPLLSLTGEPLPRAALFVHVAVTNRRGGGKAQRRGLSVRRARRSREHVSLRHTGIRPLDEAFRPAATPLRESPSLCVCVQNATLCFKELCGLPPASNLKQCIQSLAPRLQTPEGPPCATLVLKEGYPCLEPLGSLSDTTRKLLTAYDTMIAEHKQLIENADGVWEKIAQVQRDGMEFHEELQKLGEREGLKERKLSKAVESFTWNITVLKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M945</accession>
    <name>W5M945_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">V-set and transmembrane domain containing 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M945"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004904"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004912.1">
        <property type="protein sequence ID" value="ENSLOCP00000004904.1"/>
        <property type="gene ID" value="ENSLOCG00000004090.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUVT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008566"/>
    <dbReference type="InParanoid" id="W5M945"/>
    <dbReference type="OMA" id="GDFCLAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004090">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001953">
        <property type="entry name" value="V-set and transmembrane domain containing 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF8">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867477" evidence="5">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="37327" checksum="39D1DA1473516DD3" modified="2014-04-16" version="1">MKISSVVIVLLTRALIGEVCGALNVTVSPGPITVSMEGENVTLSCHVSQRKKSNSHLVIRWVFSLVSGEEHLIVKMNMRRVQVYGNDTRRFNQQKQMLVEETAGTIYNLVLLNISQEDKGHYMCKVQEIRRHRNRWRASSNGTAATELRVRVSPVVRGNEGIWRLFEDVYLCAVLICSVGIMCVFVFTVILICQCLHRKSRLKASYYLVKSPQNSSGETVTSVVSSSPVLPKKEKKFKKKKSKENADVPPEIPAKAPIADMPRRPKLLKAQPRKAVLPRIAEESLTYAELELVKPQPETRAACTGTVYAQILFEEKQLCCNLFDLIISKSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9L6</accession>
    <name>W5M9L6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dolichyldiphosphatase</fullName>
            <ecNumber evidence="11">3.6.1.43</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a di-trans,poly-cis-dolichyl diphosphate + H2O = a di-trans,poly-cis-dolichyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14385"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="EC" id="3.6.1.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dolichyldiphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.43" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01025375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640716.1">
        <property type="nucleotide sequence ID" value="XM_006640653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9L6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005075"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005083.1">
        <property type="protein sequence ID" value="ENSLOCP00000005075.1"/>
        <property type="gene ID" value="ENSLOCG00000004245.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691369"/>
    <dbReference type="KEGG" id="loc:102691369"/>
    <dbReference type="CTD" id="57171"/>
    <dbReference type="eggNOG" id="KOG3146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013112"/>
    <dbReference type="HOGENOM" id="CLU_074922_1_2_1"/>
    <dbReference type="InParanoid" id="W5M9L6"/>
    <dbReference type="OMA" id="VYATLIW"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004245">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03382">
        <property type="entry name" value="PAP2_dolichyldiphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000003">
        <property type="entry name" value="Dolichyldiphosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039667">
        <property type="entry name" value="Dolichyldiphosphatase_PAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF1">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="12">
        <location>
            <begin position="63"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="27449" checksum="933C53C1D04C5323" modified="2014-04-16" version="1">MAAAEECSVPPRWQSVSLTHVEYPSGDLTGELLAYSSLLPIFILVSFVTLIVFKRELHTISFFGGLVLNEGVNWVLKHIFREPRPCEGAHTTLTTEYGMPSSHSQFIWFFFIYSFLFLYLRMHQTNNARCLELLWRHILSICLLTVALSVSYSRVYLLYHSWSQVIYGGVAGSIIGAVWFFITQELLTPLFPKIAAWPVSEFFLIRDTSLIPNILWFEYTVTRAEARNRQRKLGTKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9N4</accession>
    <name>W5M9N4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. BTN/MOG family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9N4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005093"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005101.1">
        <property type="protein sequence ID" value="ENSLOCP00000005093.1"/>
        <property type="gene ID" value="ENSLOCG00000004258.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSRZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271914"/>
    <dbReference type="HOGENOM" id="CLU_013137_22_2_1"/>
    <dbReference type="InParanoid" id="W5M9N4"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004258">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00098">
        <property type="entry name" value="IgC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002490">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF130">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865898" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="41"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="146"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="292"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="51233" checksum="F5B346B90EC57781" modified="2014-04-16" version="1">MKSDQLKWLHVTFLLVHQCSVSQSDTFRVFGPSAPVVVFRGEDTVLPCYLSPNISAVNLEIRWFREDYTAPVCLYRYGRYNFNNQITSYRGRAELFPEEFKKSNVSLKLKSVRHSDHGLYKCMVKSQQWYEEANIYLAVRALGTHPSVSLLPPGREQSQLLCRTEGWYPEPVVIWTDRDGNNVTSLSTTTAKQDSQGLFSVSSYIPVNQESTVFSCMVRSPLPEPDWESQLYIFVENISTCGPWALPSSVIPVASVVAVVLLVIQWKKMNGMYSSVVAAVLAEHADRLNKYQLGVNQLVKIAYDTKIGSCHLTLDPNTVHCLLSLSEKNRKVTRGEKQPYPDHPERFDFWQQVLCGEGLTGRCYWEAEWSGDWAYIGVTYKGIKRKGEGDDTHLGHNDKSWCLQRWGSNSSAWHNNSKTDITVPLTKKTGVYLDWPVGTLSFYSVSPEIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9S5</accession>
    <name>W5M9S5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="14">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family. USP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01010528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9S5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005134"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005142.1">
        <property type="protein sequence ID" value="ENSLOCP00000005134.1"/>
        <property type="gene ID" value="ENSLOCG00000004297.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1871">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074994"/>
    <dbReference type="HOGENOM" id="CLU_008279_4_1_1"/>
    <dbReference type="InParanoid" id="W5M9S5"/>
    <dbReference type="OMA" id="RTCGSPQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004297">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000015">
        <property type="entry name" value="Ubiquitin specific peptidase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="481"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="130"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="265"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="619"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="903" mass="97282" checksum="C37EFD7D0CC067EB" modified="2014-04-16" version="1">MASHSSQYIFGEFSPDEFNQFFVTPRCYVELPPFNDKVPRVSQSSGRYCTPAVPFLTETMKLQVCGEDYQRIEFGVDEVIEPEPVGVKDSMYKVSSTLNPQAPEFILGCQPSQKVLDSLQASGSREFDSVDCQDSELPALDGSGACQDMDGAPGSLGPRERKKKKKRPPGYYNYLEGSSSSSSSNGGNSNSGGGAQAHSDAQLVVGLVNGHAVGTASLGSEEIGGEPAAAAAVSGAELPRGSPASGGAPAVAATGSIATVSVTNNQRTCESPDESSSDFTSGAASLSDSNNAVSSSSSSHSSGVAEGGRTAEQPPEPSSLENPELLASRRDSPSPTSLLLSSAASASATTDAAHAAAAATTDEEEGGHSGAPNGLPAPESQDSGEAPEDSAEPRGAANPPKSWASLFHNSKPSPGGPAAYVEVKSAPAAVVATQATAQAAEKPGEVMEGPVHVSEDPMAPKIAEMIENVKLIHKPVSLQPRGLINKGNWCYINATLQALIACPPMYHLMKSIPMFSETQRPCSSTPMIDNFVHLVNEFSNMPVPSKAKQQAAGDKIMKDIRPGAPFEPTYIYRLLTVIKSSLSEKGRQEDAEEYLGFILNGLHEEMLALKKLIAPQEEKVSTINGPEPQLGAEEENTEKEENGSEDEWEQVGPRNKSSVTRHADFIRTPITDIFGGHIRSVVYQQSSKESATLQPFFSLQLDIQSEKIRTVQEALETLVARESVQGYTTKTKQEIEISRRVTLEELPPVLVLHLKRFVYEKTGGCQKLVKNIEYPVDLEISKDLLSPGVRGKIFKGQRTYRLFAVVYHHGNSATGGHYTTDVFHIGLNGWLRIDDQAVKVINQFFVTKPTAERTAYLLYYRRVDLLNTARLLLLPPALPLLFPESRLFQRLLYFSFFWGGKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAG5</accession>
    <name>W5MAG5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Unc-13 homolog B</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005374"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005382.1">
        <property type="protein sequence ID" value="ENSLOCP00000005374.1"/>
        <property type="gene ID" value="ENSLOCG00000004466.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154929"/>
    <dbReference type="InParanoid" id="W5MAG5"/>
    <dbReference type="OMA" id="NHQDHLN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004466">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019992">
        <property type="term" value="F:diacylglycerol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061789">
        <property type="term" value="P:dense core granule priming"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016081">
        <property type="term" value="P:synaptic vesicle docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20859">
        <property type="entry name" value="C1_Munc13-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08394">
        <property type="entry name" value="C2A_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04027">
        <property type="entry name" value="C2B_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08395">
        <property type="entry name" value="C2C_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1100:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000002">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000014">
        <property type="entry name" value="protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000031">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027080">
        <property type="entry name" value="Unc-13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037302">
        <property type="entry name" value="Unc-13_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480:SF8">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01145">
        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="568"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="674"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="18">
        <location>
            <begin position="1107"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="19">
        <location>
            <begin position="1357"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1523"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="191"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="260"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="395"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1700" mass="192993" checksum="E26FE1B763E7810F" modified="2014-04-16" version="1">MSLLCVRVKKAKLHGPPDKFNAYVTLKVQNVKSTTITVRGDQPCWEQDFMFEINRLDLGLIVEVWNKGLIWDTMVGTVWIPLHSIRQSDEEGPGEWAALDAEVLMKEDEIYGTKNPTPHRILLDTRFELPFDIPEEEARYWTSKLEQINTMRLHDEYSLQQEVQKKPLPSAASQCCNWSYFGWGEQQTLEDHDSAVDDRDSDYRSETSNSIPPQYHTTAQPNSSLHQYPVPPRLQHQADSRESCADSMQSYDLDYRSKISRLSSQSSTRKDSSGDDQSASLSKDEKPTDVKDANSLNKQPDSSETSSVGDNKTQGKNESKEDPQVVAVDVTSQWKNEKGLVTEKICEDSQKGETLKKDTTVPVPISVRQPHPTSGLSMLDKILYKEACVSLDQKTENDSSQTFDPESKQLPEEKNVPQKEHKTNTETGWFSSLFKITSNDRTSMSLTPSKQDVGPPPFSPSRARWLRAINKVRVQLQEGREDGDPSKQPWIKGGPGGGLFGIDSMPDLRKKKPIPLVSDLAMSLVQSRKAGITSAMATRTSLKDEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFDVIRDVFAISKVAHAQQMKTIKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPIWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHHMTDVQGGGVVKIPDAKGDDAWKVYFDEVAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSKERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCQDLYSREYQTDPNKQEVPTDEQGPSIKNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELSVGKVSAEVMWSLFALDMKYAMEEHEKQRLCKSADYMNLHFKVKWLYNEYVKELPALKGSVPEYPAWFEQFVLQWLDENEEVSLEFLHGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPTIVGHYNRRFAKTIAKVLMQYADIVTKSFQTYCFKEKIPCILMNNIQQLRVQLEKMFEAMGAKQLDPEASDILNELQVKLNNVLDELSTVFGNSFQPRIEECVKQMGDLLSQVKGTGNVPASARNTVAADADNVLRPLMDFLDGNLMLFATVCEKTVLKRVLKDLWRIVMNTLEKAIVLPPLTDTSKNNTNLKNFQGTQLIFSAAKELGHLSKLKDHMVRDETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQTTDTLIKTFVQTQTAQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTSGMFRPFVEVTMIGPHMSDKKRKFTTKSKNNSWAPKYNETFHFILGNEDGPECYELQVCVKDYCFAREDRVLGIAVMQLRDLADKGSCACWCPLGRRVHMDETGLTVLRILSQRTNDEVAREFVKLKSEIRSAEEISGLEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAN8</accession>
    <name>W5MAN8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptic vesicle 2-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640277.1">
        <property type="nucleotide sequence ID" value="XM_006640214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAN8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005447"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005455.1">
        <property type="protein sequence ID" value="ENSLOCP00000005447.1"/>
        <property type="gene ID" value="ENSLOCG00000004555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688199"/>
    <dbReference type="KEGG" id="loc:102688199"/>
    <dbReference type="CTD" id="559162"/>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155403"/>
    <dbReference type="HOGENOM" id="CLU_001265_46_0_1"/>
    <dbReference type="InParanoid" id="W5MAN8"/>
    <dbReference type="OMA" id="LLWFIWM"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004555">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17441">
        <property type="entry name" value="MFS_SVOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000084">
        <property type="entry name" value="Synaptic vesicle 2-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047969">
        <property type="entry name" value="SVOP-like_MFS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="86"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="518"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="548" mass="61077" checksum="3F206502039A4349" modified="2014-04-16" version="1">MDDDLFQLRQLPVVKFRRTGESARSEDDIMCGEHEVQIEGVRAPLDPVELGDGAAVPREFANPTDDTFMVEDAVEAIGFGKFQWKLSILTGLSWMADAMEMMILSILAPQLHCEWRLPSWQVAFLTSVVFIGMMISSSLWGNISDKYGRKTGLKMSVLWTMYYGILSAFAPVYGWILFLRALVGFGIGGTPQSVTLYAEFLPMKSRAACILLIEMFWALGTVFEVLLAIMVMPTLGWRWLLGFSTVPLILFAFLCFWLPESARYDVLTGNQEKALHTLKRIATENGAPMPLGKLIAARQESRGKIQDLFSPQFRWTTVLLWFIWFSNAFSYYGLVLLTTELFQAGDVCGVSKGRRMEPRCSLECKYLSTDDYKDLLWTTLSEFPGLIVTLWVIDRIGRKKTMALCFFIFSICILPMFACVGRTALTVFIFIARAFIAGGFQAAYVYTPEVYPTATRALGLGTSSGMARVGALITPFISQVMLESSVYLTLSVYCSCCILAAIASCLLPIETTGRGLQESSHREWGQEMMGRASHRSGGIPQSSSGSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBE3</accession>
    <name>W5MBE3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="AHAT01019513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBE3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005702"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005710.1">
        <property type="protein sequence ID" value="ENSLOCP00000005702.1"/>
        <property type="gene ID" value="ENSLOCG00000004755.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008744"/>
    <dbReference type="HOGENOM" id="CLU_009629_2_1_1"/>
    <dbReference type="InParanoid" id="W5MBE3"/>
    <dbReference type="OMA" id="WFDQWFG"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004755">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="12"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="49786" checksum="1A3837D006B43E43" modified="2014-04-16" version="1">MQRAVAVIGGGMSGLSACYHLTRSPRISQVALLEASGRLGGWLRSTRREDGAIFEHGPRGVRPAGAVGRSTLDMVSELGLEGEVLPVLASHEASKNRFLYVGGQLHRMPSGVGGLVRTVPPFSRPLAVSVLKELLTRRGDQEDESIHGFVERRLGKELADIVMDCLCRGVFAGDCRELSLRSCFPPLFRAERDWGSVVLGLIRGGGDARLPVRRRLARGSRRRRGSHVGMILPPNDLNCHMGPRPHLCHGVPNNLAQYHVGPLSDARVVLSARHAFPVSLWLQFCSLAHRPARILPCIQSVTVAVVNLEYEGSVLPFTGFGHLVPSFENGALLGIVYDSVAFPQHNRRGDPSTRLTVRPNGLISQIYTGSILSRTHNDLVQRVSCSTMVDRQAATAAPIFQVYQTISHLVPLLLTEKIRHYISHHSLPLSLVGASFEGVSVNDVIRSGQMAAERIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBQ5</accession>
    <name>W5MBQ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Epoxide hydrolase 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBQ5"/>
    <dbReference type="FunCoup" id="W5MBQ5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005814"/>
    <dbReference type="MEROPS" id="S33.978"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005822.1">
        <property type="protein sequence ID" value="ENSLOCP00000005814.1"/>
        <property type="gene ID" value="ENSLOCG00000004839.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687771"/>
    <dbReference type="KEGG" id="loc:102687771"/>
    <dbReference type="CTD" id="253152"/>
    <dbReference type="eggNOG" id="KOG4178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156233"/>
    <dbReference type="HOGENOM" id="CLU_020336_7_3_1"/>
    <dbReference type="InParanoid" id="W5MBQ5"/>
    <dbReference type="OMA" id="YRWMVRS"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004839">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="98"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="369" mass="42453" checksum="E46674875489C7BF" modified="2014-04-16" version="1">MARLLHNLLLLAARLTLKIRVIGYWSLIYSYCGVCASVALLKLLWTILQKPSKTFQWGVREAPPACLNDTSLGTHCYVRIKESGLRFHYVAAGERGKPLLLFLHGFPEFWFSWRHQLREFKSEFRVVAVDMRGYGESDVPAGAENYRLEHLITDVRDIVECLGYNRCHLVGHDWGGMVAWLFAIHYPEMVTKLIVLNCPHPSVFADYALRRPLQLAKCSCIFFFQLPRLPELMLSVDDFKALKGFFPSRGAGVRWKSSSLRSEDTEAYLYVFSQPGALTGPLNYYRNIFSSLPLTQQEVRSSVLLLWGERDALLEQEMAEASRLYVRNHLRLNIISGAGHWLQQEQPDIVNTLMWTFLKEGEGWRCCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC57</accession>
    <name>W5MC57_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Polyamine-transporting ATPase 13A3</fullName>
            <ecNumber evidence="17">7.6.2.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Putrescine transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">ATP-driven pump involved in endocytosis-dependent polyamine transport. Uses ATP as an energy source to transfer polyamine precursor putrescine from the endosomal compartment to the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>putrescine(out) + ATP + H2O = putrescine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29995"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:29996"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.16" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01015179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC57"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005966"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005974.1">
        <property type="protein sequence ID" value="ENSLOCP00000005966.1"/>
        <property type="gene ID" value="ENSLOCG00000004949.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155941"/>
    <dbReference type="HOGENOM" id="CLU_001828_0_0_1"/>
    <dbReference type="InParanoid" id="W5MC57"/>
    <dbReference type="OMA" id="FSCFQYM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004949">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015594">
        <property type="term" value="F:ABC-type putrescine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902047">
        <property type="term" value="P:polyamine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000026">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000017">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000026">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000045">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF12">
        <property type="entry name" value="POLYAMINE-TRANSPORTING ATPASE 13A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="940"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="974"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1086"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1116"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1158"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="163"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1242"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1243"/>
            <end position="1257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1270" mass="142460" checksum="155842122799AC52" modified="2014-04-16" version="1">GLSWTMEKEDMKIVNKGQEDEMEISGYTLCRWKLALVGVGVVCTGGFLLLLLYWMPEWFVKSTCTRTTIREAKMVLLRSTDEFRTWFRAKVRVMLAPGKSPFDSLDSQDNTTLANGHTPHATDSPSRGFVKKYAEYQPVQVRYFTLHSTTYYWNDMVQNFEVLKGLEDLEVTCSSIHDDHSMGLTKDQQEYRRLFFGVNEIAVKVPSVFKLLIKEVLNPFYIFQLFSVILWSADEYYYYAGAIVFMSVISIATSLYTIKKQYVMLHDMVATHSIVRVSVCRANNEIEEALSTDLVPGDIMVIPSNGSIMPCDAVLVSGTCIVNESMLTGESVPVTKTNLPNPGKGVKGAEDDSIYSTEEHKRHTLFCGTNVIQTRFYSGELVKAVVVRTGFNTAKGQLVRSILYPKPTDFKLYRDAYLFLLCLVGVAGIGFIYSIVLSIMHKVPAKYIIIESLDIITITVPPALPAAMTAGIVYAQRRLKHIGIFCISPQRINICGQLNLVCFDKTGTLTEDGLDLWGIQRVESGRFLLSEENAYKEALVKSHFVACMATCHSLTKIEGVLSGDPLDLKMFEATGWILEEATEEETALHNRIMPTVVRPPKQLLPEPLVSTEQDMELYELPPSYEIGIVRQFPFSSALQRMSVVVRLLGEKRMDAYLKGAPEVIASLCKMETVPENFSDVLEGYTKQGFRVIALAHRKLESKLTWHKVQNINRDAIEVNMEFLGMIVMQNKLKTETPGVLEDLRSANIRTVMVTGDNMLTAISVARDCGMILPQDRVIIADALPPKDGQAAKINWHYADFPTKLASAPAAHPDEIQIKLDDGSPEDELNHVEHYHFAMSGKSFAVITEHFQDLLQKLVLHGTVFARMAPDQKTQLVEALQSVDYYVGMCGDGANDCGALKRAHSGISLSELEASVASPFTSKTPNISCVPSLIREGRAALITSFCVFKFMALYSIIQYISVTLLYSILSNLGDFQFLFIDVAIILLIVFTMSLNPAWTELVARRPPSGLISGPLLFSVLAQILICLGFQTLAFLWVKSQPWYETWAPESEHSACNMSMFTTENITHGHNKTDQEEDDHNIKNYENTTLFFVSSFQYLTVAIVFSKGKPFRQPSYKNWPFVLSAFSLYAFLLFILFYPIQAIDDFLEIVCVPYKWRITMLFIIIVNSAVSALVETFILDIVLWKLVFSRDKQGSSGITSASQTPQMGVDLRGPKVLSWLCCGKRWEPKARYMHLAQELLVDPDWPPKPKTTTEAKSHPSPENGSCPIISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCL8</accession>
    <name>W5MCL8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018358">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCL8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006135.1">
        <property type="protein sequence ID" value="ENSLOCP00000006127.1"/>
        <property type="gene ID" value="ENSLOCG00000005089.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2855565_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCL8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005089">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867946" evidence="1">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="65" mass="7451" checksum="4E7C53EA5AE147BD" modified="2014-04-16" version="1">LVRISLCTLVLLGLLLLFSKYGPKNTTKEKHHQLSFQQFYLFSSCPLLPLQYSSTNFSTHCHTCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCN6</accession>
    <name>W5MCN6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LIM domain only 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018358">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCN6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006145"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006153.1">
        <property type="protein sequence ID" value="ENSLOCP00000006145.1"/>
        <property type="gene ID" value="ENSLOCG00000005105.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157730"/>
    <dbReference type="HOGENOM" id="CLU_001357_7_1_1"/>
    <dbReference type="InParanoid" id="W5MCN6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005105">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09386">
        <property type="entry name" value="LIM1_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09387">
        <property type="entry name" value="LIM2_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000015">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000051">
        <property type="entry name" value="LIM domain transcription factor LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF5">
        <property type="entry name" value="LIM DOMAIN TRANSCRIPTION FACTOR LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="87"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="167" mass="18257" checksum="F43DA98B34364858" modified="2014-04-16" version="1">TMVNPGSSAQPPPVGAGSLSWKKCAGCGGKIADRFLLYTMDSYWHSRCLKCSYCQAQLGEIGTSCYTKSGMILCRNDYISRLFGNSGACSACGQSIPASELVMRAQGNVYHLKCFTCSTCRNRLVPGDRFHYINGNLFCEHDRPTALMNGHLNSLQTNPLLPDQKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD10</accession>
    <name>W5MD10_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01035853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD10"/>
    <dbReference type="FunCoup" id="W5MD10">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006269"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006277.1">
        <property type="protein sequence ID" value="ENSLOCP00000006269.1"/>
        <property type="gene ID" value="ENSLOCG00000005207.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161518"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_2_1"/>
    <dbReference type="InParanoid" id="W5MD10"/>
    <dbReference type="OMA" id="DQPWYFS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005207">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000167">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000458">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000229">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="59"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="18">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="238"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="503" mass="57785" checksum="583195B954AC4F3D" modified="2014-04-16" version="1">VFARGDCDIFGMENCCRKCMPCFRVFWDRIWPEFHYPVSRSAEILTVSGDVKIPLPKEKPVQFYEALFDFEARSEEELSVKEGDKLSVIEKLGDYVFAQKLSGSLESGLVPANYVTVIRDEFTNHRWYYGNISRQQAEKLLLSSPNKSGSFLVRISESNSDEYSISARNEGKVQHFRVYRSPYGAYYVSNKKQFATLEELICHYQANCKSLGVPFTQACVQQREHLNADSWEKPREEFVLQRKLGEGHFGEVWQGIWTPKKQKVAIKILKQEDMKQDEFVKEVQALKSLHHPKLIQLVAICSKGEPVYIITELMTRGNLKNYLTTPEGHRLSSAHLVYMASQVAEGMAYLEDRNIVHRDLAARNILVGDDLMCKVADFGLARIIKDNVYISNKRMQIPVRWTAPEAAVHQQFSPKSDVWSFGILLYEMITHGKLPYDGMSNRDVLEALGKGYRLPCPARCSQTIYKIMLSCWNKVSTNRPSFHALRSQLDTIYTKMNIKTIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEI6</accession>
    <name>W5MEI6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEI6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006795"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006803.1">
        <property type="protein sequence ID" value="ENSLOCP00000006795.1"/>
        <property type="gene ID" value="ENSLOCG00000005613.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163793"/>
    <dbReference type="InParanoid" id="W5MEI6"/>
    <dbReference type="OMA" id="MWRPPVP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005613">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000254">
        <property type="term" value="F:C-4 methylsterol oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="139"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="277" mass="32705" checksum="B49077E07B940978" modified="2014-04-16" version="1">LKISNKMASLCPITLLGPYLDYMKESLLQPLWTYLRTNHQEVLRSPLFPVIISVSTYFMACLLYMTLDSLSLVVPGINSYRIHPDQPVKLQSILKAIWLTVYNHVVFVFPAAAAQWYWRPPLPLEEKAPTVLEFTMGILGCTLLFDFQYYFWHMIHHKIQWLYVTFHAIHHEYFQPFCWVTQYMSAWELVSVGFWTTIDPIILQCHILTGYAFMVFNIWISVDDHSGYDFPWSLHNWIPFGLWGGSVKHDTHHQKPMTNFEPFFSHWDWLCGTHSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG26</accession>
    <name>W5MG26_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">STAM binding protein like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67C family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630431.2">
        <property type="nucleotide sequence ID" value="XM_006630368.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202168.1">
        <property type="nucleotide sequence ID" value="XM_015346682.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202169.1">
        <property type="nucleotide sequence ID" value="XM_015346683.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202170.1">
        <property type="nucleotide sequence ID" value="XM_015346684.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202171.1">
        <property type="nucleotide sequence ID" value="XM_015346685.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069045033.1">
        <property type="nucleotide sequence ID" value="XM_069188932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG26"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007335"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007343.1">
        <property type="protein sequence ID" value="ENSLOCP00000007335.1"/>
        <property type="gene ID" value="ENSLOCG00000006073.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682838"/>
    <dbReference type="KEGG" id="loc:102682838"/>
    <dbReference type="CTD" id="57559"/>
    <dbReference type="eggNOG" id="KOG2880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153710"/>
    <dbReference type="HOGENOM" id="CLU_023304_0_1_1"/>
    <dbReference type="InParanoid" id="W5MG26"/>
    <dbReference type="OMA" id="QHKNKCK"/>
    <dbReference type="OrthoDB" id="3640at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006073">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08066">
        <property type="entry name" value="MPN_AMSH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000010">
        <property type="entry name" value="AMSH-like protease isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000034">
        <property type="entry name" value="STAM binding protein like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044098">
        <property type="entry name" value="STAMBP/STALP-like_MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947:SF7">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="271"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="438" mass="49903" checksum="68FA8150D6070BAD" modified="2014-04-16" version="1">MDALGRTDPPNLQPTGNMDQTFSFSTLKKLAAEPDHTDVSLPAVERVRALSKMGCNIEINEDITPRRYFRSGVEMERMAAVYHEEGNLENAFVLYNKFITLFVEKLPSHRDYQQCTVPEKQVIMKKLQEAFPRTEKLKKLLQEKYNKEYMQHVKNQSKAVEHSSLQMQKLSLLEEEKQRVAQLRKMQIESEQFRYFEDQLRRQEQANKKDEAVQKVPEQTDGSCLARPPKNSSRLDHSHKNSQSPPVNRAFKSSATLGAIQDQKVEGLRSVVVPRDLSYKFLLLADSNTARGIETCGILCGKLTHNEFMITHVIVPKQSAGPDYCDMENVEELFSFQDQLDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHNDTGVFRLTSTGMGEVAGCKMKGFHPHSKDPPLFNVCNHVVVKDMKTTVLDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGX6</accession>
    <name>W5MGX6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGX6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007635"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007644.1">
        <property type="protein sequence ID" value="ENSLOCP00000007635.1"/>
        <property type="gene ID" value="ENSLOCG00000006325.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SM7S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158915"/>
    <dbReference type="HOGENOM" id="CLU_840815_0_0_1"/>
    <dbReference type="InParanoid" id="W5MGX6"/>
    <dbReference type="OMA" id="WSAWSHE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006325">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000414">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="46"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="150"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="41936" checksum="460BE10480EB5F13" modified="2014-04-16" version="1">ATLCLFSPFTRYSVQLRTRFHGNSRHWSEWSSALETRTEEAAPAVGPQLWRREGAVSADGRRRVTLQWKPLLQSQANGKILGYNVTCCWGGAPQGQEQQECGVLPPQRTSCVLVLPAPLCSCSLSAFNAAGHSPPARIALPDAGERVLPPPMALTVTPLDDSSLSVRWTPPLSQSVTGFVVEWCPLSEVTPCVPHWLRLAGNVTETVITEAVEPRVRYNVSVRALRQGRGGAALSLPAYSRQGAPSAGPRLRVTDTWIGGVALQWDPIPIHLRHGFIRNYTVCYCGADNRTQREYRCPAARVCASVCVLTYRIHVTASTDAGSAAGEAVTVAMVSEDGMSPVVTTSLYCVALLLLAWPDALCVSSRIKKQLWPVVPDPANSSLASWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH20</accession>
    <name>W5MH20_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin subunit alpha 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019666">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH20"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007679"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007688.1">
        <property type="protein sequence ID" value="ENSLOCP00000007679.1"/>
        <property type="gene ID" value="ENSLOCG00000006355.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158423"/>
    <dbReference type="HOGENOM" id="CLU_004111_2_0_1"/>
    <dbReference type="InParanoid" id="W5MH20"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006355">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034680">
        <property type="term" value="C:integrin alpha10-beta1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000005">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF26">
        <property type="entry name" value="INTEGRIN ALPHA-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012271870" evidence="15">
        <location>
            <begin position="16"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1094"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="449"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="512"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="574"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="16">
        <location>
            <begin position="619"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="17">
        <location>
            <begin position="765"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1141" mass="125763" checksum="AEC65E6377A52053" modified="2014-04-16" version="1">LRASVSFSLLGLCLCFNVDVKRPRIFSGPADALFGYSVLQHEAEGQKWMLVGAPWDGPANNRKGDVYKCIVGQERNSNCTKVNLGEVALQNVSRQLRNSHLGMTLSPSRPDGFVACAPLWSQECGTSVFSTGICASVDNSFQPKETIAPTAQSTTQEPVPGPARISHGSRSWPRWVRSMHHSQVGIAQCRTRVFSSGQSGTEIGHTTQVSPVQSCVICHGTSSGVQGRGMRTQPTSASGHASLPRRGKRVGIDPETQRRVEGLSCFPRQSGIWEHWSCGACFLCVIAQLENEPSEKFLLPENTEDERAERDIITISERKPFCPCNSCGFMPVEKHLDFSVSHFCSKLQGSVSVCLVFFVVSVYDCSAPCLQVGLLQYGFSVPDRDRGRPTSRFPVHILQKAPKIPFASFSNCSVGDTWLDTPSLCLPKFPSATEGRSAGRRIPGEGSWRLYNKVSVSQIGSYFGSEVCPVDVNRDGVTDVLLVAAPMYLGSGNKEAGRVYVYTLSRVLFTPNGTLQAHRKAQDARFGYAMAMVPDLNHDGYNDLLVGAPLEDGHRGAVYIYHGYGTSIVPQYKQRIPGSALSPGLQYFGRSVSSRLDLDGDELLDLAVGAQGSAVLLSSRSIVQINVTLSFQPHSINVIHKNCQRGVRESACLNATVCFHTTSRSPGTDRAPFDLHVGAMLDDRKFMTRALFDQSSQRQIQTVLQAQIDEVTCSMLPFHVFDTADYIRPIGFALRFRINDTESGPVLDEGWPTFAKRLIPFFKDCGEDDVCVTDLVLHAHMDVSGSRQEPYIVRSPRRRLAVELVLENRQENAYNTSLRLLFSRNLHFSSLSSKDEPQVKMECTALSSNSHSCNVSYPVFRSQAKVCALCLDCLFLSCVWTLSCVCPSVCFHSDSAEREETLHDNTAQLLAMVLYEPDLFITSDSNLNRYEVHPTRTPSEGIGPEFHTNFKVQNLGCYPVSNLELRVRLPSLAAADGPFLSVSEVTTQNVSGVNCSVLSDPRALRQAQRDVRPVHPGDLQHAEQLECQRASGLTTLSQQNGLLTLTPRQAKFKAVKIFSSHSLAVPESSMLSLGPAGLWRETVLEVLKGRSIPISLWILIGSIIGGLLLLALIIFLLWKLGFFSRKQREEREGECREYGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH29</accession>
    <name>W5MH29_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tumor protein p53 inducible nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH29"/>
    <dbReference type="FunCoup" id="W5MH29">
        <property type="interactions" value="646"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007688"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007697.1">
        <property type="protein sequence ID" value="ENSLOCP00000007688.1"/>
        <property type="gene ID" value="ENSLOCG00000006359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTG4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063829"/>
    <dbReference type="InParanoid" id="W5MH29"/>
    <dbReference type="OMA" id="TVHLHQP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006359">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029431">
        <property type="entry name" value="TP53INP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671">
        <property type="entry name" value="DIABETES AND OBESITY REGULATED, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671:SF0">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14839">
        <property type="entry name" value="DOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="243" mass="27264" checksum="0F15DFC7E26CB0BF" modified="2014-04-16" version="1">MFQRFTSILFGDVAEDGSSCKRAPEFSEKEDDDEWILVDYLDTCTNTCREGESVICAQEDSSLDSPALFSSISSLASTRELVDTGFLELNPCTLEESWFITPPPCFTAGGQAPGLLETSPMENLLIEHPSMSVYAVHNLHNTLKDTARRSRDQSFNSFSSRMEPQNRTGQHIGCYAAALSAHTGLFEQAKYVRLSQRTKDNAEKQHLSRSTLRRHNLVRDGGSRQVKSSAVFVHQPTQRQFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHE5</accession>
    <name>W5MHE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHE5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007804"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007814.1">
        <property type="protein sequence ID" value="ENSLOCP00000007804.1"/>
        <property type="gene ID" value="ENSLOCG00000006449.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRPK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162662"/>
    <dbReference type="HOGENOM" id="CLU_003826_4_1_1"/>
    <dbReference type="InParanoid" id="W5MHE5"/>
    <dbReference type="OMA" id="YSSMPCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006449">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="720" mass="78563" checksum="623B4C5A77A0A1F6" modified="2014-04-16" version="1">AREEYDDAMSSGQQAFLLEESDESPDVFRLSVGSLPPGQSASVSLSYVTELPLQEDGALRFCLPGVLNPRYTPAGGESVTAEAPGVLGGAVPYTLALGAHISSPSSIDRVQSNCPLSPLEYLAEDKSQAKVRVAQHRDAVQPAVCFIHWKVTSGKLLHVFFFIELRFMDLLLKTLLVLKPCWPVALGLELSSTGLMGPESDVLCHWALGSAVSSVKDTLLLLLKSLPLGCYFNIYGFGSTHESFFPESVEYSQDTMDKALAKVKTMQADLGGTEILRPLTEIYHKPCLPAHPRQLFVFTDGEVGNTKQVTDLVKTNSLSHRCFTFGIGEGASSALIRGMATEGSGHFQFITGTERLQPKVMQSLRFALQPAVREISVRWDVPSGLEVSSLSPPLKALFQGQRALLYAQLKGQCDHSAEGSVLLEYKLGDEPYKSKLSFSLKPDKESGLTIHRLGARSLLRSLEREEREQEGEEEKKAVRKRATELSTQSGVVCSHTAFIGVNKENKQPVQGPVLRRNIPRYQPEEMAYKNVAPHRVSSHFAPQVPNNHLGRHFLEIALVEHQGKTVGYQDCSQQNGSRLHLQLSSTGINNADVTLAPTGGRPSCAGAPEPQENPLLKLISLQKANGSWDLDEALLAVLGKKKKEVASKMPGKGLDKTAWATLLALLWLHGFKLDSRDEWQFVALKAVSWIKAQPGLDVAQCVQAGATLLGCQVDPQSLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI03</accession>
    <name>W5MI03_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Endophilin-A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Endophilin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Implicated in endocytosis. May recruit other proteins to membranes with high curvature.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI03"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008012"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008022.1">
        <property type="protein sequence ID" value="ENSLOCP00000008012.1"/>
        <property type="gene ID" value="ENSLOCG00000006631.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154737"/>
    <dbReference type="HOGENOM" id="CLU_047887_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI03"/>
    <dbReference type="OMA" id="MFPANYC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006631">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07592">
        <property type="entry name" value="BAR_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF63">
        <property type="entry name" value="ENDOPHILIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="20">
        <location>
            <begin position="18"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="311"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="373" mass="41789" checksum="7F6835E2EB3F9D90" modified="2014-04-16" version="1">MSVAGLKKQFYKASQMVSEKVGGAEGTKLDEEFKDLERKVDVTSKAVTEVISKTSEYLQPNPASRAKLSMLNTVSKIRGQVKSPGYPQAEGLLGECMVKYGRELGEDTNFGGALLDAGESMKRLAEVKDSLDIDVKQNFIDPLQGLCDKDLREIQHHLKKLEGRRLDYDYKKKRQGKIPDEEVRQALDKFHESKEVAETSMYNLLETDIEQVSQLSALVEAQLQYHRQAVQILEELSEKLRTSMSEASSRPRREYTPKPKPSLDYGDADQSNGGFYTPAAAAAPAARSSAWHQDFPGPVVPLSLPPFPHTMDQPCCKALYDFEPENDGELGFREGDIITLTNQIDENWYEGMLHGQSGFFPLNYVEVLVPLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIC9</accession>
    <name>W5MIC9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIC9"/>
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        <property type="protein sequence ID" value="ENSLOCP00000008138.1"/>
        <property type="gene ID" value="ENSLOCG00000006738.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000228622"/>
    <dbReference type="HOGENOM" id="CLU_929201_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIC9"/>
    <dbReference type="OMA" id="DSILTQC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006738">
        <property type="expression patterns" value="Expressed in liver"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052949">
        <property type="entry name" value="PA_immunity-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42999:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42999">
        <property type="entry name" value="ANTIBIOTIC RESISTANCE PROTEIN MCBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23625">
        <property type="entry name" value="UIM_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="300" mass="31416" checksum="FF7B970095C8C237" modified="2014-04-16" version="1">QPQVVLITGTLALGIPAFHCNLISSVECALENRVWAECALEDSILTQCALENRVWAECALEDSILTQCALEDSILTQCALEDTVWAECVLENSILTQCALENRVWAECALEDSILTQCALEDSILTQCALENGVWAECALEDSILTQCALEDSILTQCALEDSTPTQCALEDSVLKDGALEAGVLTEDVLEAGALTEEDVLEAGALTDGALEAFALIEEVLEAGALTDNALDAGALTEDVLDAGALTEDVLDAEDVLDAGALTEDVLDAGVLQNGVLEIGALTDGALEAGALTEDVLESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJT4</accession>
    <name>W5MJT4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJT4"/>
    <dbReference type="FunCoup" id="W5MJT4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008643"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008653.1">
        <property type="protein sequence ID" value="ENSLOCP00000008643.1"/>
        <property type="gene ID" value="ENSLOCG00000007141.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6TB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162189"/>
    <dbReference type="HOGENOM" id="CLU_138624_1_0_1"/>
    <dbReference type="InParanoid" id="W5MJT4"/>
    <dbReference type="OMA" id="SEMEHAP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007141">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0048306">
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        <property type="entry name" value="S-100A10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein S100"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
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        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="125" mass="13960" checksum="B0D2150A445713F8" modified="2014-04-16" version="1">NGVPARMSVCVCASLCVCESHSANMPSDLERTMETLITVFHRYADKDGDSNTLSKRELKELMEKELSSFLKSQKDPGAIDKIMRDLDQNGDGQVSFEEFVSLVVGLSIACEQCYQLHIKKQQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJY4</accession>
    <name>W5MJY4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032391">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008693"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008703.1">
        <property type="protein sequence ID" value="ENSLOCP00000008693.1"/>
        <property type="gene ID" value="ENSLOCG00000007182.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1226">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286983"/>
    <dbReference type="HOGENOM" id="CLU_011772_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJY4"/>
    <dbReference type="OMA" id="CECSMDT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007182">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000009">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF36">
        <property type="entry name" value="INTEGRIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0401">Integrin</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="17">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="18">
        <location>
            <begin position="564"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="610" mass="67125" checksum="BFA0C3DD14DA21C9" modified="2014-04-16" version="1">LPAGEPHSFTVNFKRAEGYPIDLYYLMDLSYSMKDDLENIKKLGQEILSTLQKVTNSVRIGFGCFVDKVAMPYVSTVKSKLRNPCPTRMDTCQPAFSFRNVLPLTDNAKEFEKKVSTQNISGNLDPPEAGLDALMQAAVCQEQIGWKNVTRILVYTSDDTFHTAGDGKLAGIYFPNDGLCHLNSEGLYEKSTEYDYPSVGHLSQVLAANNIQLIFAVNDKSVPIYKALHKLIPQSVVGELKDDSSNVVQLISDAYNNLSSTIYLEHQQLPQGLDITYDSHCGDGSTARGQRRAECTNIKINQAINFTVTVNSTACLDQPQTFVIKPQGINEELYVTVETLCHCDCKDHQPAXCRATNTSAPCSGRGDCMCGLCMCHGNSLHGQYCQCDDASCDRHNNKICGGNGQCECGKCRCNPGFDGTACECSTATDACGGGSGELCSGNGVCECNRCRCKEGYYGQLCTGCHNCKDDCEKFNECVTCKAKGTENCNSDCMGVQVKIVEEVKEFSCRVGSYVFRIERDSSTGLPAVIYFSDAPTTIDKTYVIVGSAVSGIILIGLAIIILYRISVEVYDRREYQNFLKEQQNVRWKETHNPLFKDATTTVVNPMFQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK60</accession>
    <name>W5MK60_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of G protein signaling 16</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK60"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008769"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008781.1">
        <property type="protein sequence ID" value="ENSLOCP00000008769.1"/>
        <property type="gene ID" value="ENSLOCG00000007246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697560"/>
    <dbReference type="KEGG" id="loc:102697560"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164081"/>
    <dbReference type="HOGENOM" id="CLU_059863_3_0_1"/>
    <dbReference type="InParanoid" id="W5MK60"/>
    <dbReference type="OMA" id="DHETRNI"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007246">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RGS"/>
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        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF245">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="57"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="192" mass="22177" checksum="E1F48EC596A51B7D" modified="2014-04-16" version="1">MCRGLASLPTTCLERAKELRARLGIFCQKADSSHSEYSNKADQVRPTPDEALKWKESFVTLLKNKHGLATFRKFLILEFSEENIAFYLACEDYKIPKSTSKLDAMAKKIYEEFIESDAPREVNIDHETRNITKFNLVKPSISCFDLAQNKIYTLMEKDSYPRFLRSALYQDLIKKATYKSAKQQISSKKSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKU8</accession>
    <name>W5MKU8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01014376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626816.2">
        <property type="nucleotide sequence ID" value="XM_006626753.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKU8"/>
    <dbReference type="FunCoup" id="W5MKU8">
        <property type="interactions" value="1835"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009007"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009018.1">
        <property type="protein sequence ID" value="ENSLOCP00000009007.1"/>
        <property type="gene ID" value="ENSLOCG00000007420.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695514"/>
    <dbReference type="KEGG" id="loc:102695514"/>
    <dbReference type="CTD" id="246175"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157298"/>
    <dbReference type="HOGENOM" id="CLU_016428_4_2_1"/>
    <dbReference type="InParanoid" id="W5MKU8"/>
    <dbReference type="OMA" id="PHYYARA"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007420">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000002">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000008">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF35">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="16" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="21">
        <location>
            <begin position="233"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="592" mass="67372" checksum="CDD4F247EF990DA2" modified="2014-04-16" version="1">MKLHFSQELAKGGGVARNGQQQDFRKIRLYACKFNAKSSLFTECRLIGMPKEKYEPPKLYTIMSAEEVANGKKSHWAELKISGRVRSLSSSLWMLTHLTALHLNDNSLTRIPPDIAKLQNLVYLNLSSNKLRSLPAELGNMVSLRELLLNNNLLRFLPYELGRLFQLQTLGLKGNPLSQDILNLYQEPNGTRKLLNYMLDNLAVYPEQLPQRPWITVKERDQMIPTAVFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEMVNSDADIISLQEVETEQYYTLFLQALKERGYDGFFCPKSRAKLVSDQERKHVDGCAIFFKTDKFTLVQKHTVEFNQVAMANSEGSEVMLNRVMTKDNVGVAVLLEVKEIFVGGMEPFPEKQLLLVANAHMHWDPEYSDVKLIQTMMFISELKNIVKKASNSAKSDSACDPGSIPIVLCADLNSLPDSGVVEYLSNGGVAENHKDFKELRYNECLMNFSCNGKNGNSDGCFTHNFQLKSAYESNLMPYTNYTYVFKGVIDYIFYSSTHMSVLGLLGPLDPQWLIDNIITGCPHPHIPSDHFTLLTQLELHPRLPSVNGLHLPIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLJ2</accession>
    <name>W5MLJ2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phospholipase A2</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01005210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLJ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009251"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009262.1">
        <property type="protein sequence ID" value="ENSLOCP00000009251.1"/>
        <property type="gene ID" value="ENSLOCG00000007625.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTYI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165179"/>
    <dbReference type="HOGENOM" id="CLU_026553_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLJ2"/>
    <dbReference type="OMA" id="HKIAPHE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007625">
        <property type="expression patterns" value="Expressed in ovary and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04704">
        <property type="entry name" value="PLA2_bee_venom_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000002">
        <property type="entry name" value="Phospholipase A2 group III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866278" description="phospholipase A2" evidence="11">
        <location>
            <begin position="24"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="12">
        <location>
            <begin position="172"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="12">
        <location>
            <begin position="393"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="541" mass="60736" checksum="A328833F365668AA" modified="2014-04-16" version="1">MQIRSELLLALFVVFGSVSVVFAEDDVIRSETAGESLDFCYLIRRSASRPGHMYYSFLRRTHFGSLLLYQSTWTEGQRLADCAASGDKTLTDSYWSLCRAEGRSNFSSIPDPRIDTSLLFGPGSPCVTKPVMDVGGNSAQRAKRDLETLKQGHTAASSPSETPRRKKRGWFIPGTLWCGTGTSAATYGDLGVFEETDTCCREHDQCENTISSFQINFGVFNHNLFTVSHCECDRRFRQCLLGVNDTVSNLVGYGFFNVLKVPCFVFEPKLQCVQMYWWGGCKSSKIAPFATFQKSVAYNSTHPAEEEQHMGWWPQARGSGVHGNSTSTPAPPAGNTTTGKPAGRPQPARKFIPLLHAVTHGRSNESSSPSKPHRQLDLENSNVLENKVLDTLYSDLESKWQSCNCYKHLDQCKYKIGPQEVKFSLKNPEPKTLYHCNCTRRMARRVGRLSEWNAVESLLSDYVAMSCFQLQQTENCMKQEGCVAVLSRATHLKRVLKDRRTITGGAPKVKRRSARPAKSKAIPRRLYSRCLRIIDSKKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM35</accession>
    <name>W5MM35_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anaphase promoting complex subunit 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM35"/>
    <dbReference type="FunCoup" id="W5MM35">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009444"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009455.1">
        <property type="protein sequence ID" value="ENSLOCP00000009444.1"/>
        <property type="gene ID" value="ENSLOCG00000007778.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZUK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000938"/>
    <dbReference type="HOGENOM" id="CLU_142923_0_0_1"/>
    <dbReference type="InParanoid" id="W5MM35"/>
    <dbReference type="OMA" id="EGTDQDQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007778">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026182">
        <property type="entry name" value="ANAPC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22526:SF2">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15243">
        <property type="entry name" value="ANAPC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047039586" evidence="7">
        <location>
            <begin position="28"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="134" mass="16100" checksum="B69BB814DA47AED7" modified="2025-10-08" version="2">MLYFWWSFLHLRMSTLFPSLFPRMTESLWFNLDRPCVDENELQQQEQQHQTWLQSIAEKDNNLMPIGKPVSEQTYDDEDEEDDEDEEDSEEDSEDDEDMQDIDEMNDYNESPDDGEINEVDMEGADQDQDQWMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMR3</accession>
    <name>W5MMR3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Complement component C7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C7 serves as a membrane anchor. During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer or dimer; as a C5b-7 complex it can also form multimeric rosettes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMR3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009672"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009683.1">
        <property type="protein sequence ID" value="ENSLOCP00000009672.1"/>
        <property type="gene ID" value="ENSLOCG00000007953.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU3G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156804"/>
    <dbReference type="InParanoid" id="W5MMR3"/>
    <dbReference type="OMA" id="CQNGGQP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007953">
        <property type="expression patterns" value="Expressed in heart and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:complement activation"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:complement activation, classical pathway"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000099">
        <property type="entry name" value="Complement component C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C7_KAZAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
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        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040729">
        <property type="entry name" value="Kazal_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF2">
        <property type="entry name" value="COMPLEMENT COMPONENT C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18434">
        <property type="entry name" value="Kazal_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21330">
        <property type="entry name" value="Kazal_C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
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        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00057">
        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="13" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="18 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="17 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9 25" id="KW-0768">Sushi</keyword>
    <keyword evidence="7" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="20" id="KW-1053">Target membrane</keyword>
    <keyword evidence="10 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="28">
        <location>
            <begin position="141"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="576"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="636"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="850" mass="95355" checksum="BE61E7FFC112542B" modified="2014-04-16" version="1">IQFSVKSYAIINKYYNFAVSFYIHIVNTVAISLLLLLEVSCMSPVNCKWGPYGDWSECDGCKKTQVRKRTVETYGQYGGSPCPGESTERRACLPTRGCPLEEGCGNRFRCSSGLCISKSLVCNGDQDCEEDGADEQGCSDQSSVCDNDQPPPKVELTGKGFDVLNGKLRGSVINTKSFGGECRKVYTADHKPSYRLTQNLVKYTFQVKIKNDFSDEFYNSSWSYKKHTEYKMTSNYDGHDSRDFTTALSNEKSHRLLVIKNEVEVAQFLNGASEDLTLSENFWKDLTLLPASYEYSAYRDLIQDYGTHFMKEGYLGGQYQALLYVDSEKMKQQGISNLDAQKCTSSSSGFLFISVKRSECNKLVEALRTAEGYKRDRIRAETQIVGGRSGFASGLNYLDLNNPTANSDLYTKWAGSVTQNPSVIKQKLRPLFELVKEVPCAAVKRYNLKRAIEEYLNENHPCNCKPCQNNGIPAVKDTLCTCVCKPDTYGPACELGTLLQEQPGVIDGSWSCWSSWSSCSQDQRVRTRTCNNPFPRMGGKFCIGEPKETRPCEDEELAYLREMEPHCFDTTLEPTKSCKPPPVIQNGFVEDPKDLYPIGSKIVYSCKERYYIVGDPVAECDEDLKWKRYPVQCMRTVCQPPDFMADVKGTPWKPSYEIGEKIVMSCPAGKQLEGVSEIKCDSSLNWSPQISNTRCKAAPVKTTVRPTHQCKPWEKADKSQCICKMPYECTSSFEVCATYVKTGQAYRLSICKIHALECQGRIYNLTEENKCTSLQSQSKACPDCQLWEKCDVGTNMCVCRENEECTKSGAHICVLAGENPAAQSMTECEAGRRRCHGENISAVSIWPCEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP42</accession>
    <name>W5MP42_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01002736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP42"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010163.1">
        <property type="protein sequence ID" value="ENSLOCP00000010151.1"/>
        <property type="gene ID" value="ENSLOCG00000008346.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694922"/>
    <dbReference type="KEGG" id="loc:102694922"/>
    <dbReference type="CTD" id="28965"/>
    <dbReference type="GeneTree" id="ENSGT00940000159700"/>
    <dbReference type="HOGENOM" id="CLU_000022_46_2_1"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008346">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000005">
        <property type="entry name" value="Solute carrier family 27 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF10">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="18">
        <location>
            <begin position="61"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="19">
        <location>
            <begin position="501"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="70409" checksum="D9C4835E6ED822E4" modified="2014-04-16" version="1">MLLEWMTALIAGLVAFHFIQKHTFPYFWKDFFYLTKVARCGVILELYKKTSKVFTVMDKFMQQAEKIPNKPFIIYEGDVYTYKYVNSRSNKVAQVFKTLGIVKKGDTVALLMNNEPDFISVWFGFCKLGCAVAFLNTNLKSKSLLHCFISCGAKVLVVGADLIDKLDDILPNLQEDHISIWVLARESQRPGVETLLDKVDIAPDGPVPAHLRAASSVKSPTLYIFTSGTTGLPKAAVITHLQSLKACAGFWAYGATKDDIIYIPLPLYHSAASLLGIGGTIELGATCILKKKFSASQFWSDCKKYNVTLFQYIGELCRYLCNQPKKEGEKDHKVRMAVGNGMRQDIWEEFLDRFGNIKMCELYGSTEGNVCFMNHTGKIGAIGRSNFFYKLFFQYELVKYDLENDEPVRNEQGFCEHVKNGETGLLLSQINSKSPFFGYVGNKNLTEKKLLRDVFRKGDVYFNTGDLLAEDHENFIYFKDRIGDTFRWKGENVATIEVADFLGWMDFIQEANVYGVEVPGHEGKTGMAAIILRPGFSFDGGRLYEHVVKHLPNYARPRFIRIQELMEVTGTLKQQKFRLVQSGYNPSSVTDPLYFLDESKKCYVALTQTIFDCIVSGQMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPN1</accession>
    <name>W5MPN1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Brambleberry</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634272.2">
        <property type="nucleotide sequence ID" value="XM_006634209.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPN1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010340"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010353.1">
        <property type="protein sequence ID" value="ENSLOCP00000010340.1"/>
        <property type="gene ID" value="ENSLOCG00000008496.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687361"/>
    <dbReference type="KEGG" id="loc:102687361"/>
    <dbReference type="CTD" id="559540"/>
    <dbReference type="eggNOG" id="ENOG502QRBT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115898"/>
    <dbReference type="HOGENOM" id="CLU_027987_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPN1"/>
    <dbReference type="OMA" id="DHSMSEF"/>
    <dbReference type="OrthoDB" id="5978806at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008496">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061472">
        <property type="term" value="P:karyomere membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007344">
        <property type="term" value="P:pronuclear fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040346">
        <property type="entry name" value="GEX1/Brambleberry"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538:SF1">
        <property type="entry name" value="PROTEIN BRAMBLEBERRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868530" evidence="3">
        <location>
            <begin position="29"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="607" mass="66808" checksum="B76337EC93135600" modified="2014-04-16" version="1">MMLMAAGLRLMSALFLWGLCTHVRPARAFFGWMTKKAAPSPAPPPPSFSEAEGGAPPPFEMSVADEHFLAEAKQLELSPLDTCHYRVIAKIKTTCSSLSEEELAKLGVVLFNCQAEVEGRRTFPCTEEMTIKECTAEMDSDTWNAYHIVSNRARSVCYATRQQHFRRKAELTVNSLISSAANQLEAMKTLKEGQLELKEVTASSVEKLLLSHGALLEQQGALQNGQQQLDSAINSNLERLTQEKELISSGQELVAQLIQRITQRMENVSEHLKGQGLELQDGHRAILADLAEVRGQAKDIYKKIEQNLSGFLLYQNRTAHFYDDLMGKLQRMNSTLGLLVHYLDVMQGRLEDRLHSIQGYLGWAGCSLAAVSTCVLHGGYFLLSAVLMSFLHTPGFSRVALLVMVLFNAVAEINQQPALDFTSLSLLLLLVSVGHWFLLQLFRGCVTLHRRRSASPPMLCPGNTASETEILKFGCLTSSTPDRDPGEAGVKLEQDSYIFDEPSMAGVSRPPQTTPSHSTPLFKSRRSLPGVALEGIRQRNLVTLLDAVSDSRSSSPNVSTASIGSMSGRQLCNGVTKMGQVCKKRAVLGQDYCRLHEGGHTSYTPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPN8</accession>
    <name>W5MPN8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204384.1">
        <property type="nucleotide sequence ID" value="XM_015348898.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204385.1">
        <property type="nucleotide sequence ID" value="XM_015348899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPN8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010347"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010360.1">
        <property type="protein sequence ID" value="ENSLOCP00000010347.1"/>
        <property type="gene ID" value="ENSLOCG00000008504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696570"/>
    <dbReference type="KEGG" id="loc:102696570"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234559"/>
    <dbReference type="HOGENOM" id="CLU_045991_3_0_1"/>
    <dbReference type="InParanoid" id="W5MPN8"/>
    <dbReference type="OMA" id="GMCQFLG"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008504">
        <property type="expression patterns" value="Expressed in muscle tissue and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15144">
        <property type="entry name" value="7tmA_PGE2_EP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000384">
        <property type="entry name" value="Prostaglandin E receptor 1a (subtype EP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001105">
        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF3">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP1 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="212"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="59"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="368" mass="40574" checksum="081F809659A05249" modified="2014-04-16" version="1">MMLAVHQFNTSGAGQDAANSRNHTWMLGDNMSVVDERVFAYKPTDPTAAGLSMTLGILSNIIALFILAKAYARMRRRSKATFLLFASSLVATDFAGHIIPGALVLRLYLVPDSYASSDALCQFLGGCMVFFGLCPLFLGCAMAAERCLGVTRPLLHSALVTSTRTKLSLAVIWLFALCVALLPCFQLGAYGMQYPGTWCFIKVDSDTTASDVIFVLLFSGLGLTSLGVSMVCNTTSGLTLVLARLRKRTYNRRAKSHDIEMVVQLVGIMVTSCICWSPLLIFGMISVTKSYSNCEVDLQYYKQLMVLGVRLASWNQILDPWVYILLRRAVLRKIYTIAKGQSNLKGSTFRRWEISSLQGSEKMAVNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ34</accession>
    <name>W5MQ34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cofactor required for Sp1 transcriptional activation subunit 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Mediator complex subunit 26</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ34"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010493"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010508.1">
        <property type="protein sequence ID" value="ENSLOCP00000010493.1"/>
        <property type="gene ID" value="ENSLOCG00000008633.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691943"/>
    <dbReference type="KEGG" id="loc:102691943"/>
    <dbReference type="eggNOG" id="KOG1105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000259"/>
    <dbReference type="HOGENOM" id="CLU_478915_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQ34"/>
    <dbReference type="OrthoDB" id="550309at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008633">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042376">
        <property type="entry name" value="MED26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031416">
        <property type="entry name" value="Med26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201">
        <property type="entry name" value="CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15693">
        <property type="entry name" value="Med26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="534" mass="59651" checksum="7FD070E4C98DE7F8" modified="2014-04-16" version="1">MALISKETCSPQQMKELLLQSMDRDANVVNMEKVLEVLSMLENYPITREALEETRLGKYVNSLRKKTLKKELSKRAKKLVKSWQKIALIEESTPASVCADRKNSPVQQMTPGISGTPAEQHRFSSVVDPDKCNIPPSFTEMHILHHTAWVRVDEAAVDSPEVIPSPTSEPMGQSGHRHFVRQETGGFLEKGTLEWPQSPSSDNSSLQQRKLKSWARVIQEPGSLETFSPASCSTPPHSPVTATSDYSLVHRYAHSSSFLTIPPSQHPQRTSHFTQQHSAIVHKAASSSPVDILSTSTHPVAEGKSQALSPPSSVGEEQVETKPKSKRGRRKGGFKDVSVCLDGLPPEPGPVKIKERRITYDPLRRKIVVVTPKTSAPSKAEHESPLPKDTGSISELRERNLQIWDSMDKSDWKEFAKNKIIQKYFSSQCKRLTVSGLEPKEYIDHLQELLSQDSIASEGWQPCVIVPTEPASHLPGVSQEVSIEDLFQIHSQHWSGVNGCYDSKSNWYDWAQCISLEPYSNKEQLGILPYVCLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR89</accession>
    <name>W5MR89_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger MYND-type containing 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR89"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010898"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010914.1">
        <property type="protein sequence ID" value="ENSLOCP00000010898.1"/>
        <property type="gene ID" value="ENSLOCG00000008947.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691818"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000527"/>
    <dbReference type="HOGENOM" id="CLU_022430_0_0_1"/>
    <dbReference type="InParanoid" id="W5MR89"/>
    <dbReference type="OMA" id="FTECSAY"/>
    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008947">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042989">
        <property type="entry name" value="ZMY15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47085">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47085:SF1">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="193"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="606"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="641" mass="71521" checksum="18EC320DA6B38BE4" modified="2014-04-16" version="1">METVTGYRDPLIDFCDAMSRWFCCYRAAVCGRTKEESRGADGNVKRCPRLPVDQASPHWFLHVLDCSPVSAKVKPWLSLASLDQSDLECTVQRLFLVTDSAGVPLGFDLLEGGTKGNVPVLRIVGLLRQCMEAPLGRGGARRPLTLTVGDRQIHRILSGNALSALGVELSKQCFTGWAPKEGFALRWPPIRHCHVCKKHAFEAPLRPCPKCQAVLYCSEQCVQADWSKSPADVSHQHCCPLLGKFMSHCSQLADMPFTYSAETTSPDFDVEHFLSSRGLNSGYWLQQSMLVHSAFQEVEPVRKQPRESSPAELAAPRQPFGPLQESEMMLSCSPPTAPSLNTPLVSWLQYYKWRGFDLSSPVAVLLSCPLTIYYIITSLVPRNFPELNILKKQSLKIHIIESEREFNMLLTFWELSDLLPHVTFELLFVGEKLPKDYDEQQLLLQRKDGHVTLESPSMASEEKTDKKSIRVRICSRAYHLFQGPKPNLVIGFRSGFGLGDSWLSTLPRLQALKVPAYFCEPSEVSCVSSQQVMSTATGGSLSPPTLNPFHCPLRITSRDSYLPCYSNAFVFHLIYKPSPAAKRDHAAAAPERPLLPHVNAACLPSASPSGALQEDVSKDGRVKLSRKERKQAARNTSRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRA9</accession>
    <name>W5MRA9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.155</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-[beta-D-GlcNAc-(1-&gt;4)]-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-[beta-D-GlcNAc-(1-&gt;4)]-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-[beta-D-GlcNAc-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16921"/>
            <dbReference type="Rhea" id="RHEA-COMP:14374"/>
            <dbReference type="Rhea" id="RHEA-COMP:14377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:139507"/>
            <dbReference type="ChEBI" id="CHEBI:139510"/>
            <dbReference type="EC" id="2.4.1.155"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 18 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.155" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01036577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRA9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010918"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010934.1">
        <property type="protein sequence ID" value="ENSLOCP00000010918.1"/>
        <property type="gene ID" value="ENSLOCG00000008962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTNG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153470"/>
    <dbReference type="HOGENOM" id="CLU_016749_1_0_1"/>
    <dbReference type="InParanoid" id="W5MRA9"/>
    <dbReference type="OMA" id="VTHQNFC"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008962">
        <property type="expression patterns" value="Expressed in embryo and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030144">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026116">
        <property type="entry name" value="GT18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052105">
        <property type="entry name" value="MGAT5_Glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15075:SF7">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 6-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15075">
        <property type="entry name" value="ALPHA-MANNOSIDE BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15024">
        <property type="entry name" value="Glyco_transf_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase family 18 catalytic" evidence="15">
        <location>
            <begin position="42"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF15024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="647" mass="72741" checksum="327504D6C2AA1544" modified="2014-04-16" version="1">LLIPTCPEGQPQVCEVPNNPKFPLCAGKVQQFLQTSWHSDPCYAFYGVDGTDCSILAYLSEREDFCPTLPSRSLAIVPDWLQESTREKREALIRTDLTPLFEVIGNSRGPAVRFMLSRIKRLSRRWAKAGEKIRQKIGHRPQQQLRVLLYPGVLAGGAGQRFGEMVEKGGPLGELVQWADVSTALYVLGHHLIFASSQPLLRSLIGAAPGKGSCPIESPLPFDLLYTDYHGLAQLQAAMGLSFHHYRCRYRVLDSFGTEPAFNLREYADQHSYSTAWGSWNLQPLQYMTMFPHTPDNSFLGFVTEELGRDLEEQEQTRRKANIAVIYGKQDYMWQQGKESYLDVLSQELEVHGTVYQEPGSSLHLPSYIINHGLLPQDQLLSLLRRAKLFVGLGFPYEGPAPLEAIASGCVFLQPRFQPPRSPQNSDFYRGKPTTRQVPSQHPYAENFIGKPYVWTVDMSNSTEVRLAVRSILHTQVEPLVPYEFTCEGMLERVFAYISHQVNQNCSIRMATFCIKETSRPLSFHSPTDFCSDSHPSWPPETALLPRLGSAGKSCTAVCHDASLVCEPALFHLLNNATAFSRMGANCSGTSAEVNHLFPAYNQRRRRCFLQREKLLFSCAGSGSLYRRLCPCRNYQQGQVALCKGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSM5</accession>
    <name>W5MSM5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSM5"/>
    <dbReference type="FunCoup" id="W5MSM5">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011384"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011400.1">
        <property type="protein sequence ID" value="ENSLOCP00000011384.1"/>
        <property type="gene ID" value="ENSLOCG00000009330.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234641"/>
    <dbReference type="InParanoid" id="W5MSM5"/>
    <dbReference type="OMA" id="GTTIFWQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009330">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015746">
        <property type="term" value="P:citrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF17">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="35596" checksum="DDDC747087792B3B" modified="2014-04-16" version="1">LRVMADNATCPPFQLGRPRYDQSSFYGRLRHYLDIVDPRTLLVSESRLQECVKLLDDFRCGTLPPGVSDAQLWEAQKIKQAIVHPDTEEKIFMPFRMSGYVPFGTPIVVGLLLPNQTLASTVFWQWLNQSHNACVNFANRNATKPTPVSRFLQGYAGAVTSAVSIAVRIGIMFSRGYQRLTGTNHLVSCPSTASANICNVVLMRHTELAEGIDVRDSSGNIVGSSKIAAKCALLETALTRVVLPMPILVLPPLVMAFLEKLPLLRAHQRLVLPVHSLVCLASFGLALPLAISLFPQISQVEVSKLEPEIAMATDCEVVTYNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSX1</accession>
    <name>W5MSX1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ubiquitin-related modifier</fullName>
            <shortName evidence="7">SUMO</shortName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636708.1">
        <property type="nucleotide sequence ID" value="XM_006636645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSX1"/>
    <dbReference type="FunCoup" id="W5MSX1">
        <property type="interactions" value="1555"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011480"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011497.1">
        <property type="protein sequence ID" value="ENSLOCP00000011480.1"/>
        <property type="gene ID" value="ENSLOCG00000009407.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682389"/>
    <dbReference type="KEGG" id="loc:102682389"/>
    <dbReference type="eggNOG" id="KOG1769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182895"/>
    <dbReference type="HOGENOM" id="CLU_148322_2_1_1"/>
    <dbReference type="InParanoid" id="W5MSX1"/>
    <dbReference type="OMA" id="SKMMNAY"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009407">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16115">
        <property type="entry name" value="Ubl_SUMO2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000022">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="15"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="94" mass="10723" checksum="ED5AEA4DB1276AC5" modified="2014-04-16" version="1">MSEEKPKEAVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSIRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU97</accession>
    <name>W5MU97_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane 4 L six family member 19</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638053.2">
        <property type="nucleotide sequence ID" value="XM_006637990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU97"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011956"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011977.1">
        <property type="protein sequence ID" value="ENSLOCP00000011956.1"/>
        <property type="gene ID" value="ENSLOCG00000009786.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682454"/>
    <dbReference type="KEGG" id="loc:102682454"/>
    <dbReference type="eggNOG" id="ENOG502S1UJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234590"/>
    <dbReference type="HOGENOM" id="CLU_087168_0_0_1"/>
    <dbReference type="InParanoid" id="W5MU97"/>
    <dbReference type="OMA" id="IINGCFG"/>
    <dbReference type="OrthoDB" id="9897613at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009786">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF22">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="200" mass="21510" checksum="3661A34C79C0F916" modified="2014-04-16" version="1">MCIARCGKALGILLIPLALVSMLANTLLLFPNLQSRYLREGHITKYATLGTGLWASGLLVLPAARVFITSSHKKGRCGFRSEMLCSILYAVIAMAGATLCFVTSGTALVEGPLCKYNETYSNGTQEQVWGTPLEHKTPSPSSWEEVCLEPQGVVLWNVVLFSTLLASSGTQALLCIIQVLNAILGFLCGPDIKHNKVSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUC2</accession>
    <name>W5MUC2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TNFAIP3 interacting protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUC2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011981"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012002.1">
        <property type="protein sequence ID" value="ENSLOCP00000011981.1"/>
        <property type="gene ID" value="ENSLOCG00000009803.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPYT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046908"/>
    <dbReference type="InParanoid" id="W5MUC2"/>
    <dbReference type="OMA" id="PDYQWHA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009803">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.990">
        <property type="entry name" value="Nemo cc2-lz domain - 1d5 darpin complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31882:SF2">
        <property type="entry name" value="TNFAIP3-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31882">
        <property type="entry name" value="TNFAIP3-INTERACTING PROTEIN COILED COIL FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="165"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="38253" checksum="51C127705812FD71" modified="2014-04-16" version="1">EELKQQLRILQKQRQELLDVNKKWDEQYKLMKQHYSLKIMEMKERLTVCDKFITEVNAEQDKKQKDFDKKLVLAKEKIEKEQEKNTKLSAELCEIKQQSLNLKEQNATLTKRKDHQECEIQRLNKVLQKSVQNRHSYSNISISKFLPGLGNPARNIFGEETATQIEVLRHQAQIYENDFKEERRDRERLKERNEELEKRCKKLQSELQILKSQRMLGQTEKKSTGKNTAQQVKQFPLSTAKQWYPPPYCCSHWAAFPPHGTVCALPHCSNPSNLCHLQDRHGDRQQHPPNYQWYVSASDQLPPDVPQPRNLPNKEANGKTKTHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUF4</accession>
    <name>W5MUF4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-iminobutanoate/2-iminopropanoate deaminase</fullName>
            <ecNumber evidence="8">3.5.99.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Translation inhibitor L-PSP ribonuclease</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex. Acts by bridging YTHDF2 and the ribonuclease P/MRP complex. RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif: cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-iminobutanoate + H2O = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:39975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:76545"/>
            <dbReference type="EC" id="3.5.99.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2-iminopropanoate + H2O = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:40671"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:44400"/>
            <dbReference type="EC" id="3.5.99.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotrimer. Interacts with YTHDF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.10" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01004716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUF4"/>
    <dbReference type="FunCoup" id="W5MUF4">
        <property type="interactions" value="1034"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012013"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012034.1">
        <property type="protein sequence ID" value="ENSLOCP00000012013.1"/>
        <property type="gene ID" value="ENSLOCG00000009827.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687981"/>
    <dbReference type="KEGG" id="loc:102687981"/>
    <dbReference type="CTD" id="10247"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00420000029792"/>
    <dbReference type="HOGENOM" id="CLU_100715_7_1_1"/>
    <dbReference type="InParanoid" id="W5MUF4"/>
    <dbReference type="OMA" id="GSYFKEP"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009827">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120241">
        <property type="term" value="F:2-iminobutanoate/2-iminopropanoate deaminase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000008">
        <property type="entry name" value="ribonuclease UK114 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF39">
        <property type="entry name" value="2-IMINOBUTANOATE_2-IMINOPROPANOATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="137" mass="14381" checksum="8BC2415556BBEEFB" modified="2014-04-16" version="1">MSAIVRRIIGTAKAPAAIGPYSQAVLVDRTMYISGQLGMDPSTGQIVPGGVTAEARQALANMGEILKTAGCDYNNVVKTTVLLADINDFNGVNDVYKQFFRSHFPARAAYQVAALPRGGRVEIEAVAVLGPIADASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWT1</accession>
    <name>W5MWT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylhydrolase ARH1</fullName>
            <ecNumber evidence="4">3.2.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ADP-ribose-L-arginine cleaving enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">[Protein ADP-ribosylarginine] hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.19" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01028349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWT1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012840"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012866.1">
        <property type="protein sequence ID" value="ENSLOCP00000012840.1"/>
        <property type="gene ID" value="ENSLOCG00000010474.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692964"/>
    <dbReference type="eggNOG" id="ENOG502QPMI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063627"/>
    <dbReference type="HOGENOM" id="CLU_047061_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWT1"/>
    <dbReference type="OMA" id="RKWEFLQ"/>
    <dbReference type="OrthoDB" id="10250509at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010474">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003875">
        <property type="term" value="F:ADP-ribosylarginine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051725">
        <property type="term" value="P:protein de-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4080.10:FF:000002">
        <property type="entry name" value="ADP-ribosylarginine hydrolase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012108">
        <property type="entry name" value="ADP-ribosylarg_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF26">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016939">
        <property type="entry name" value="ADP_ribslarg_hdr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="393" mass="43408" checksum="879E63647459FA5E" modified="2014-04-16" version="1">MCSKETPDSFNPSVSGVDSCRLQMPFKEPDKVSNPSVTLEERYVAAMVLSGAGDALGYNNGNWEFSRSGEAIHTELTKMGGLKNIDVKDFRVSDDTVMHLATAEALVQVGNKPQLPDLYHVLAQKYIDCLDDMDGRAPGVTCIETIQFLKPHVKDGWKIPFNPKGGGCGAAMRAMCIGLRFPHPSQEEQLVAVSVESGRMSHHHPTGYLGSLATALFTSYAVQGKPLEQWGRGLLDTLENAKSYVRQSGHYTEDNLRAWDYFENQWKAYLNKRGILDGTSKARFPEVYGVKERDDFYKSLSYSGWAGSSGHDAPMIAYDGLLRAGDSWTSLADHAFFHGGDSDSTAVIAAACWGAMYGFQGVPKCNYGNLEYRKRLEQLGKQLYKLSQTEKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXZ0</accession>
    <name>W5MXZ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dihydropyrimidinase like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXZ0"/>
    <dbReference type="FunCoup" id="W5MXZ0">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013277.1">
        <property type="protein sequence ID" value="ENSLOCP00000013249.1"/>
        <property type="gene ID" value="ENSLOCG00000010797.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234527"/>
    <dbReference type="HOGENOM" id="CLU_015572_2_2_1"/>
    <dbReference type="InParanoid" id="W5MXZ0"/>
    <dbReference type="OMA" id="WVTAEVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010797">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048936">
        <property type="term" value="P:peripheral nervous system neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050770">
        <property type="term" value="P:regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF57">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="184"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="697" mass="75407" checksum="BFE017DE55C270B5" modified="2014-04-16" version="1">MIEMLRKAHFGFGIMAERKRTWDKEDDLPVYLARPGTTDQVPRQKYGGMFCSVEGAFESKTIDFDALSVGRRSSRSSKSGSKGEASQEGKSPDSEQSSICTESGSGGHEEGDSGIEIKTAVGKEILQNLDELKSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKVIEANGKMVIPGGIDIHTHFQMPYRGTTTVDDFSQGTKAALAGGTTMIVDHVIPEPGSSLIEAFERWREWADDKACCDYSLHVDITHWNDSVKQEVETLIKEKGVNSFLVYMAYKDLYQMTNSELYEIFTFLGELGAIVQVHAENGEIIAQEQARMLEMGITGPEGHVLSRPEELEAEAVFRAITIASQTNCPLYVTKVMSKSAADIISQARKKGNVVFGEPISASLGTDGTHYWSKNWAKAASFVVSPPLSPDPTTPDYLNSLLASGDLNVTGSAHCTFSTAQKAIGKDDFTQIPEGVNGVEERMAIIWDKAVVTGKMDENMFVAVTSTNAAKILNLYPRKGKIAVGSDSDLVIWDSDAVRTVTAKTHNSAAEYNVFEGMELRGAPLVVICQGKIVLEDGNLHATAGSGHFIPCSPFPDFTYKRVKARKQLAVLKAVPRGMYDGPVSDFSPMSRGGTPAASARTSPTKQAPPVRNLHQSGFSLAGIPAAQGIPAEETPIRPAGRRIVVPPGGRSNITSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY02</accession>
    <name>W5MY02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="23"/>
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            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="92"/>
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        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="33"/>
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    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11471" checksum="9CDCDBB100D4598B" modified="2014-04-16" version="1">QRDWCILCVIYYLAFPAPRPCSAGEFQCLRGSCVPDDSICDFTDQCGDSSDENDCSASERCNFENGYCNMSHSKDSQTLWTSRNGLTNIKPTSDHLGNRTGIE</sequence>
</entry>
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    <accession>W5N146</accession>
    <name>W5N146_LEPOC</name>
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        <submittedName>
            <fullName evidence="8">Solute carrier family 36 member 1</fullName>
        </submittedName>
    </protein>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        </location>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="481" mass="53003" checksum="78D66F94755D4B7C" modified="2014-04-16" version="1">MTTDGANSINGTMASSKADTPNEETPLSPSHSLSPSESQDQYERIGGRAGTSFFDTLIHLLKGNIGTGLLGLPLAVRNAGLVLGPLSLLVMGIVAVHCMDLLVKCSHHLSARLGLPFLDYGEAVEHGLATGPGSWLQRHSVWGRRLVNLFLIVTQLGFCCVYFVFLSDNVKQVIEAANGTTNDCHTNLTAVIVPTFDSRLYMLCFLPAIILLVFIRNLKWLAPLSLMANLSMCSSLVLVYWYSITNISYPVSLPYVGKAKDYPLFFGTAIFAFEGIGVVLPLENKMQKPASFRRVLYLGMVIVTFLYISLGTIGYMCFGDHIGGSITLNLPDCWMYQIVKLLYCFGIFITYALQFYVPAEILIPQVVARFPERWVLVIDFSIRTLLVMLTCALAILIPELDLVISLVGSISSSFLALIFPPMLEIITFHREGLSYWVITKNVVISIIGFIGFVAGTYVSIQQIVIRSAERHAHNGTMSFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2H6</accession>
    <name>W5N2H6_LEPOC</name>
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        <recommendedName>
            <fullName evidence="15">ERO1-like protein alpha</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Oxidoreductin-1-L-alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006632490.3"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000014864.1">
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        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012049">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF1">
        <property type="entry name" value="ERO1-LIKE PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866911" description="ERO1-like protein alpha" evidence="21">
        <location>
            <begin position="25"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="20">
        <location>
            <begin position="93"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="20">
        <location>
            <begin position="393"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="468" mass="54644" checksum="D07232D72BD0916C" modified="2014-04-16" version="1">MTMRFKLKLFVLLGSLLCLHTSQDFYPGSAEHRCFCQVTGHLDDCTCDVETIDTFNNNHLFPKLQELLKMDYFRFYKVNLNKPCPFWTDSSHCGLRDCAVKPCKPNEVPDGLKSASYKYTEDASRELEECEKLEQLGAVDSSLSEETRQVLLEWSRHDDEADRFCESDDEESPDSQYVDLLLNPERFTGYKGPEAWQIWNSIYEENCFKPCTVKRPLNPLTSYRGEDEGKTFYRWLEGLCVEKRAFFRLISGLHASINIHLSARYLLDDNWYDKKWGHNITEFQQRFDAELTKSEGPKRLRNLYFLYLIELRALSKVLPFFERRSFQLYTGQSAQDQRAKDLLLELLQLADSFPLHFDENSLFAGDREEATKLKEDFKLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKQIQALPESGHLRLGFQLTRQEIVALFNAFGRISTSIKELETFRKLLSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2M8</accession>
    <name>W5N2M8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 39 member 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2M8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014887"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014916.1">
        <property type="protein sequence ID" value="ENSLOCP00000014887.1"/>
        <property type="gene ID" value="ENSLOCG00000012098.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693943"/>
    <dbReference type="KEGG" id="loc:102693943"/>
    <dbReference type="CTD" id="64116"/>
    <dbReference type="eggNOG" id="KOG2693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158926"/>
    <dbReference type="HOGENOM" id="CLU_015114_13_0_1"/>
    <dbReference type="InParanoid" id="W5N2M8"/>
    <dbReference type="OMA" id="ITMFAGE"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012098">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF2">
        <property type="entry name" value="METAL CATION SYMPORTER ZIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="480" mass="53032" checksum="EA8FEE59A7CD0BF6" modified="2014-04-16" version="1">MFNTSSYRIVAFIVRTLFLIATLHILTSKATVIYKRKSFLEDIFGYYGENETFSSGKLESLLYRVGSRRPQDNVGELEALHNSQCLSSGDILSLHELNNQSRLTMSDFEKICPAILQQAVVRPCLLSTQEPEPSVPHKPVSYHVWGYGVLAVTIINMASLLGLILVPFTKKPYFPKLLTYFTGLAIGTLYSNAVLQLIPEALGFDPKKDNYVLNAVGIFGGFYVLFFTEKILQFALKPEHKQGHSHFNTSEEESNQSNANKMGTNSVTNGELTHKTEIITVTSFSCSEEPSIGTAAQTDQDIQVSRGYCRWLSGPRIRNIKTVAWMITLSDALHNFIDGLAIGASFTVSVINGVSTSIAIVCEEFPHELGDFVILLSAGMSIPQALFFNLLSACSCYIGLILGIVVGTNFSPNVIFAFAGGMFLYIALADMFLEMNNIQGDHERNTRSKIIFFTIQNLGLLTGFSVILLITMFAGNIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5N316</accession>
    <name>W5N316_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N316"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015054.1">
        <property type="protein sequence ID" value="ENSLOCP00000015025.1"/>
        <property type="gene ID" value="ENSLOCG00000012217.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4302">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:mitotic spindle midzone"/>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MAP65_Ase1_PRC1"/>
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        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
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        <property type="entry name" value="MAP65_ASE1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="26548" checksum="D8A6A60ADDEBA45D" modified="2014-04-16" version="1">MEQGKRLEEKPAGSLSDEIVMWQNELEHLEELKKANLEEVIKKIRVELAEYWDKCRFSSDQRDSFKYFYDDNFTEELLMKHDEELLQVKMYYEKCKPLLETVERWEKNFAIFQEFERRASDPNRFSNRGGTLLKEVKERVKIQKLLPKLEEELKSSIEMWEAEQGTEFLFGGLKVMDYIANHWDEHRLLKGKEKNERVSKICGGFPWCLEWSAPVSATFEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N376</accession>
    <name>W5N376_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ectonucleotide pyrophosphatase/phosphodiesterase 7, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N376"/>
    <dbReference type="FunCoup" id="W5N376">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015085"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015114.1">
        <property type="protein sequence ID" value="ENSLOCP00000015085.1"/>
        <property type="gene ID" value="ENSLOCG00000012260.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159339"/>
    <dbReference type="HOGENOM" id="CLU_017594_1_2_1"/>
    <dbReference type="InParanoid" id="W5N376"/>
    <dbReference type="OMA" id="TNEESAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012260">
        <property type="expression patterns" value="Expressed in intestine and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
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        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
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        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
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    </dbReference>
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        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868721" evidence="3">
        <location>
            <begin position="28"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="489" mass="55476" checksum="B270AB442E701C55" modified="2014-04-16" version="1">SVLIMVSMLSVCFLLASLALRAPLSSTAPCCRNKLLLISFDGFRWDYDQDVDTPNLDAMAQDGIKATYATPPFISITSPSHFTLITGKYVENHGVIHNMWFNTTTGEKKPYYMTQFVNEWWDNGSVPIWITAQRQGLKAGSLHFPGTAATYQGEKAHLSEVEPRFYDYSNETQWRENVDKVMDWFTVQHLDFVALYFGEPDSTGHKYGPDSPQRRNMVKQVDRTVGYIRSVVDQRSLTDHLNIIITADHGMSTVHRAPVVQEITLSKIPGFSFKDLQFHIVDYGPASLLLPKEGLLDKVYNALKNAHPKLHVYKKEEMPRRLHYSKNSRILPLFLYADPGYVINGLIPVQFNNGEHGFDNDAMDMKTIFRAVGPDFKKNLEVEPFETVHVYALMCHLLRIQPEPNDGSLEVTRNILQTKTNEESACSSISICFGVLTQVFIGLTAAAGFLVVVFVIITVHTVHKRRKSQHRSKKNGLDEKCPKTKQSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N470</accession>
    <name>W5N470_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">SEC24 homolog D, COPII coat complex component</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N470"/>
    <dbReference type="FunCoup" id="W5N470">
        <property type="interactions" value="1320"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015429"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015458.1">
        <property type="protein sequence ID" value="ENSLOCP00000015429.1"/>
        <property type="gene ID" value="ENSLOCG00000012522.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182924"/>
    <dbReference type="InParanoid" id="W5N470"/>
    <dbReference type="OMA" id="RLCKHGD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012522">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060536">
        <property type="term" value="P:cartilage morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070278">
        <property type="term" value="P:extracellular matrix constituent secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000023">
        <property type="entry name" value="protein transport protein Sec24C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000020">
        <property type="entry name" value="protein transport protein Sec24D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000003">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000004">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="17">
        <location>
            <begin position="363"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="18">
        <location>
            <begin position="440"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="20">
        <location>
            <begin position="690"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="19">
        <location>
            <begin position="786"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="904"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1036" mass="113244" checksum="5BE2A8E110D7E6FD" modified="2014-04-16" version="1">MSQPGYVATPPYSQAQPGIAGYPSGFGSAPSQTLYGHYGGPPQSFSPSQPGALKPSGPAASGMPSPPGPNQFSPNNLQNGAHSQRFPPSPAVSAPSLSPYGQHPQAPYQNSPIPAPTQQLTSQMNSMQISGYGHGPMQGSQPPPSPAAQQGFQTSLPQTMSHPPLPPGSQASPIASSSFTSGAHPMGAMSSVSPMGTTVPFSGPHPGNVGFQPPTAQSGPHGFLPQQAGSYGGQMAGAQPGFPGSFPAGAASQMAGPPQKKLDPDSIPSATQVIQDDQARRGGQIYATNIRGQVPPLVTTDFDVQDQGNASPRFMRCTAYSFPCTSDLAKQCQVPLATIIKPFATIPKNETPLHVVNHGETGPLRCNRCKAYMCPFMQFIEGGRRYQCGFCSCVNEVPPFYFQHLDHIGRRVDFYERPELSLGSYEFVATLDYCKNNRPPSPPAFIFMIDVSYSNIKSGLVKLVCDELKTLLEKLPRVSKGDSVALLKMFIVTYNKILHFYNVKSNLAQPQMMVVSDVAEMFVPLLDGFLVNFHESRSVINNLLDQIPEMFADTNESETVFAPVIQAGMEALKAAECSGKLFIFHSSLPTAEAPGKLKNRDDRKLVNTEKEKILFQPQKGVYEQLTKECVSSSCCVDLFLFPTQYVDVATLGAIPLHTGGTLYKYSNFQIQADGEHFLSDLRKDVEKPIGFDGIMRIRTSTGFRATDFFGAIYMNNTTDVEMAGVDCDKAVTVEFKHDDTLSEDSGALVQCALLYTTIGGQRRLRIHNLSLNCTSQLADLYKSCETDALINFFAKSAYRAMLNQPLKTVREILVSQSAHMLACYRKCCASPSAASQLILPDAMKIFPVYMNGLMKSSALVGSPELSTDERAYHRQLVMSMDVADTQVYFYPRLMPVHNVDVSSDVMPSPVRCSEERLSDGGIFLLENGQNMFLWLGQSCPPELIQSIFNVPSFAHISTEANSLPELDNPYSKKLRFIIDCITLQRPHSMKLVIVKQKDKPEMLFRQFLVEDKGLHGGASYMDFLCYVHREIRQLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5S0</accession>
    <name>W5N5S0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor RFX3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Regulatory factor X 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5S0"/>
    <dbReference type="FunCoup" id="W5N5S0">
        <property type="interactions" value="908"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015979"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016009.1">
        <property type="protein sequence ID" value="ENSLOCP00000015979.1"/>
        <property type="gene ID" value="ENSLOCG00000012975.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244879"/>
    <dbReference type="HOGENOM" id="CLU_010393_1_1_1"/>
    <dbReference type="InParanoid" id="W5N5S0"/>
    <dbReference type="OMA" id="FYRNSSM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012975">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000017">
        <property type="entry name" value="transcription factor RFX3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007668">
        <property type="entry name" value="RFX1_trans_act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF20">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04589">
        <property type="entry name" value="RFX1_trans_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="12">
        <location>
            <begin position="181"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="675"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="747" mass="83281" checksum="DA5FCF2F7B36099F" modified="2014-04-16" version="1">MQTSEAGSDTASTVTLQTTVAAQPAVPTQVVQQVPVQQQILQVQAVQQVQHVYPAQVQYVEENNAVYTNGTIDSRTYSYSEAQMYSQNTGGNYFDTQGSSSQVTTVVSSHSMGAGGIPMGIAGGQIISSSGAYLIGNSMENSAHPTTQTTRASPATIEMAIETLQKSEGLSTQRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEQKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMALRQQPVQQKQRYKPVQKVDGMGDNFAGSGQHTPTAAEQTVIAQSQHHQQFLARNTDASRALPDFVELDLGDTIMDIITPEDIKTLQTLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSVTLEGSTITENSNVSEIEGRLPKSKLILLCKNETVLKWMCNCDHVMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLTNAMNNIPQKMIQTKVAAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESVVQRLEQDFKLTLQQQSTLEQWAAWLDNVVNQVLKPYEGRPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFDDLNTMSPANIDKDEVSDAESEMEDEMEDSGEPLAKREKAEVSQALQGALDGGVQPIILNSLPPEHILPGTPTIRHCSATGNTYASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6C7</accession>
    <name>W5N6C7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
            <ecNumber evidence="15">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01000408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635250.1">
        <property type="nucleotide sequence ID" value="XM_006635187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6C7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016186"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016216.1">
        <property type="protein sequence ID" value="ENSLOCP00000016186.1"/>
        <property type="gene ID" value="ENSLOCG00000013142.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695349"/>
    <dbReference type="KEGG" id="loc:102695349"/>
    <dbReference type="CTD" id="558084"/>
    <dbReference type="eggNOG" id="KOG3704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154768"/>
    <dbReference type="InParanoid" id="W5N6C7"/>
    <dbReference type="OMA" id="YDYGLEW"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013142">
        <property type="expression patterns" value="Expressed in liver and 10 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000194">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF7">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 3B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="17">
        <location>
            <begin position="144"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="359"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
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        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="396" mass="45392" checksum="16CBBC800A635852" modified="2014-04-16" version="1">MEYSLICHNLYAISFSPVKKKLLLLCIMLLLWVYMLYSCVGYCSTMPSLMVDSYRGKDIQTAAKRTDSGGTDLMSRLLPRTQSWVDLETDYDEPPVRTASRDLLNNEIEVDRTDDWEEMRSEQRSLEPSALSSFSNGSGTKKLPQAIIIGVKKGGTRALLEFLRVHPDIRAVGAEPHFFDRNYDNGLDWYRDLMPKTLEGQITMEKTPSYFVTREAPARICAMSRDTKLIVVVRDPVTRAISDYTQTLSKKPDIPTFESLTFKNRTTGLIDTSWSAIQIGIYAKHLENWLQYFPMGQILFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNQTKGFPCLKKAEGSSKPHCLGKTKGRTHPNIDPEVVQRLRDFYRPFNMKFYQMTGHNFGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6R7</accession>
    <name>W5N6R7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Programmed cell death 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPII-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="1522"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000016356.1">
        <property type="protein sequence ID" value="ENSLOCP00000016326.1"/>
        <property type="gene ID" value="ENSLOCG00000013249.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000160982"/>
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    <dbReference type="InParanoid" id="W5N6R7"/>
    <dbReference type="OMA" id="KYRDKAT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013249">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0001525">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:response to calcium ion"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="EFh_PEF_ALG-2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF9">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="23337" checksum="64B46624E83B18EC" modified="2025-10-08" version="2">MNQSERTTQKQKYPRMAYQYRPSQYNTPPDQGFLWNIFQRVDKDRSGSISDTELQQALSNGTWTPFNPVTVRSIISMFDRDNKGGVNFNEFSGVWKYITDWQNIFRTYDRDSSGLIDKNELKQALAGFGYRLSDQFYDILIQKFDRQRKGQVAFDDFIQCCIVLQRLTDVFRRYDTDQDGWIQVSYEQYLSMVLNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6Y7</accession>
    <name>W5N6Y7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Melanin-concentrating hormone receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">MCH-1R</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="2">Interacts with NCDN.</text>
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        <subcellularLocation>
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    <sequence length="360" mass="39605" checksum="28D038DF32C8015A" modified="2014-04-16" version="1">MTYVNSSLVSKNYSSSNQTAAERGHAPYTDVIMPSIFGVICFSGIIGNCIVIYTIVKKTKFRAKQTVPDIFIFNLSIVDLLFLLGMPFLIHQLLGNGSWCFGAAMCTIITALDSNSQIVSTYILTAMTLDRYLATVHPIQSNYVRTPCVAAAVIGLVWLLSLLTIVPVWMYAGLMPLSDGSVGCALLLPNPATDAYWFTLSQFLLAFAFPLAVICAVYFKMLQHMSSSVAPLPPRSLRLRTRKVTRMAVAICLAFFVCWAPYYILQLAHLGVQRPTVAFVYAYNIAISLGYANSCINPFLYIALSETFKRRLVVAIRPASKECRVNNSTTEGSVSLRVVPESLQQCPFLGDFSHSGLPVT</sequence>
</entry>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="226" mass="25938" checksum="113EA33D2262D2AF" modified="2014-04-16" version="1">ALAVDQSPNVMIEEEHNVTLYCSQKGTSYSGMYWYRQLPGQHLELIVYYYYDTESMEKKFEGRFSSERKDSSLYLTVRELKSADSAVYFCAKQEAHCYFPPQLQQIFKRLFEEILFVESLTCLLGISGCGQELHVSQTPLILWNNTGESVSIHCEHQGGISYNAMYWFRQLPGERIQLIVYSVVGIDPDFGELSTKKYDVKKTEAQKGSFTVKNLEPGDSVTYFCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7Q5</accession>
    <name>W5N7Q5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015348271.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5N7Q5">
        <property type="interactions" value="1719"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000016694.1">
        <property type="protein sequence ID" value="ENSLOCP00000016664.1"/>
        <property type="gene ID" value="ENSLOCG00000013512.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685933"/>
    <dbReference type="KEGG" id="loc:102685933"/>
    <dbReference type="CTD" id="6161"/>
    <dbReference type="eggNOG" id="KOG0878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158890"/>
    <dbReference type="HOGENOM" id="CLU_071479_4_1_1"/>
    <dbReference type="InParanoid" id="W5N7Q5"/>
    <dbReference type="OMA" id="HPSGYEE"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013512">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="135" mass="15836" checksum="A10626A1CB7ACF06" modified="2014-04-16" version="1">MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKKNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLMMSNKTHCAEIAHNVSSKNRKIIVERAAQLAIKVTNPNARLRSEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N854</accession>
    <name>W5N854_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calcium channel, voltage-dependent, gamma subunit 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N854"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016843.1">
        <property type="protein sequence ID" value="ENSLOCP00000016813.1"/>
        <property type="gene ID" value="ENSLOCG00000013635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244961"/>
    <dbReference type="HOGENOM" id="CLU_053704_1_1_1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013635">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000003">
        <property type="entry name" value="Voltage-dependent calcium channel gamma-7 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008369">
        <property type="entry name" value="VDCC_g5su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008368">
        <property type="entry name" value="VDCC_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF4">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01792">
        <property type="entry name" value="VDCCGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01793">
        <property type="entry name" value="VDCCGAMMA5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="275" mass="30932" checksum="1C0F1145DF2B2465" modified="2014-04-16" version="1">MSTCGRKALTLLSSVFAVCGLGLLGIAVSTDYWLYLEEGIILPQNQSTEIKMSLHSGLWRVCFLAGEERGRCFTIEYIMPMNVQMTSESTVSVLKMIRSATPFPLVSLFFMFIGFVLNNIGHIRPHRTILAFVSGIFFILSGLSLVVGLVLYISSINDEMLNRTKDNEAYFSYKYGWSFAFAAISFLLTESAGVMSVYLFMKRYTAEEIYRPHPGFYRPRLSNCSDYSGQFLHPDAWVRGRSPSDISSEASLQMNSNYPALLKCPDYDQMSSSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9P8</accession>
    <name>W5N9P8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribonuclease, RNase K a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase K family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627510.1">
        <property type="nucleotide sequence ID" value="XM_006627447.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9P8"/>
    <dbReference type="FunCoup" id="W5N9P8">
        <property type="interactions" value="330"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017357"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017388.1">
        <property type="protein sequence ID" value="ENSLOCP00000017357.1"/>
        <property type="gene ID" value="ENSLOCG00000014085.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685378"/>
    <dbReference type="KEGG" id="loc:102685378"/>
    <dbReference type="eggNOG" id="ENOG502S351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009664"/>
    <dbReference type="HOGENOM" id="CLU_140554_2_1_1"/>
    <dbReference type="InParanoid" id="W5N9P8"/>
    <dbReference type="OMA" id="SNNCFIA"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014085">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="101" mass="11273" checksum="20700DB62002BC9C" modified="2014-04-16" version="1">MASLLFCGPKLAACGIVLSIWGVIMLLLLGIFFNVHSAVLIEDVPFTEDDMLLDKNPPQKIYGLYDQVSYNCFIAAAIYLLVGGLSFCQVQLNKRKEYMVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA76</accession>
    <name>W5NA76_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Heteroous nuclear ribonucleoprotein U like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000014236.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
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    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
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            <begin position="184"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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    <sequence length="804" mass="89919" checksum="B872DCF595FE041F" modified="2014-04-16" version="1">VDVKKLKVNELKEELQRRGLDTRGLKAELAERLQAALEAEASGEGGGPPAALGEAYSGGGDAGDGEDYQDEDEEEEEEENGEDGGMEGEEPLHQGYCELSADGMNEPEIKEECESEREEHQGSPEVKQEVKEELVSESAPVTEPSQPVKPKVEEEQRHTAPHGRKRPYDESRGHSYYEHREDKRSRSPQPPAEEEEEDFDDTLVAIDTYNCDLHFKVSRDRYSGYPLTIEGFAYLWSGARVSYGVTKGRVCYELKINEEISVKHLPTSEPDPHVVRVGWSLDSCSTQLGEEPFSYGYGGTGKKSTNCKFEDYGERFGENDVLGCYIDFESGEDVEMAFSKNGQWLGEGFRVRKEELAGRALFPHVLVKNCAIEFNFGQKDEPYFPIPEGYSLIQSVPLEDRVRGTVGPSSKSECEILMLVGLPAAGKTTWAMKHAEENPEKKYNILGTNAIMEKMKVMGLRRQRNYAGRWDVLIQQATQCLNRLIQIAARKRRNYILDQTNVYGSAQRRKMRPFEGFQRKAIVICPTDEDLKERTLKRTDEEGKDVPDHAVLEMKANFTLPEAGEFLDGVTYIELQGEEAEKLVKQYNEEGRKAGPPPDKRYDNRPGGFRGRGGGYQRYDSRGGPQGGRGGYQGRGGAGGGSGGYRGGYNRGGYNQNRWGSGGNHRDSASGGRGGYNRNQQSGGGYNHNRVAPYSKGGSGGYSQAQPQTYNQGAYNQGAYNQSSYNQSYNYPGYSQYPGYNQAYTQPPATTTQGYTQQQGYNQQQYQQYAQQWSQYYQNQSQWNQYYGNYGNYGNYSGSTQGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAU9</accession>
    <name>W5NAU9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Toll-like receptor 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Monomer and homodimer. Exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand. Proteolytic processing of an insertion loop (Z-loop) is required for homodimerization upon binding to the unmethylated CpG ligand leading to its activation. Interacts with MYD88 via their respective TIR domains. Interacts with BTK. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Interacts with SMPDL3B. Interacts with CD82; this interaction is essential for TLR9-dependent myddosome formation in response to CpG stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Endomembrane system</location>
            <topology evidence="18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000017758"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017790.1">
        <property type="protein sequence ID" value="ENSLOCP00000017758.1"/>
        <property type="gene ID" value="ENSLOCG00000014430.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163875"/>
    <dbReference type="HOGENOM" id="CLU_006000_2_0_1"/>
    <dbReference type="InParanoid" id="W5NAU9"/>
    <dbReference type="OMA" id="YNQNFCR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014430">
        <property type="expression patterns" value="Expressed in bone element and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Toll-like receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410:SF3">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="16" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869492" description="Toll-like receptor 9" evidence="20">
        <location>
            <begin position="30"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="21">
        <location>
            <begin position="875"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1039" mass="115453" checksum="E76F84898CF7C9C3" modified="2014-04-16" version="1">SSPQASPRGAAALPGFLAVLVSLLPPGAATYPPFLPCGSPSPQTVDCAAHGLTRVPLFRAPTGAVTALLLSGNHISRIPADAFALFRRLETLELQWNGLLRPGVPKAGGPMVLAPRAFEPLAQLRSLDLRGNGLRAVPLLPPSLRTLDLSYNRLLLLEPPARPPALAALQTLRLDGNCYYNNPCGHSQGAPRGFLAGMGRLRELSLGHNNLTAVPGGLPAGLRRLALHYNQISAVAPGDFGQLARLSSLDLKGNCRRCEHAPSPCIKCARSALHLPAGCFASLGSLKSLNLSDNSLASLNDSVFAPLRGLEELLLSDNLLSRTSPNGNDLVLGSPAFRGFPRLRSLDLSYNYRRFTVFRRLALTEDFAGLARLQSLQITGCFFQSVDNASVAPLAGLPELRLLNLRLNFIKNIELHIFDSLAGLSYLRLSDNEISCTRCRGAPGKRPGLPDHRLTPGSSLQARGLIAPEPQVGLPLPESCTGRETLNLMRNNLVYVQKELFEGFERIECLILSHNSISQSFNGNQFILLKRLKLLDLSYNRIDLYYKAAFSELPSLEVLDLSNNNYHFQLSGMGHRLDFIAGLPSLRLLDLGFDSISDRISTALASSSLNKLVFRHNRLQDMWNKKIYFQFFRNLTNLTSLDLSNNLLTRIPPEGIYHLPPTLRDLNLKFNKLTSFPWEALSAFPCLEILDLSYNWLSTLSRSLHPGSYIPPDSGLRSLNLSSNRIHAIDRDFFRNATALASLLLSDNQLEQLDFDNFLLQRLRVLDIRWNRLVCTCDSSFYELFGNFNFSVPALTSQVTCGSPDKLRGRSVFSRDAPLCPGSRNMAAFLLSLALALPLTALPVLRQVLGWDAWYAFYVWVARAWGLFYKGGEGSAYDAFVAFDKEQESVADWVYNELRVQLEDSGAQRFRLCLEERDWLPGRSSIENLHEAVQGSRKTVFVLPRAGPAGGLLREAFFLAQQRLLEERADVAVFVLLEKGRRGSRYLQLRRLLCRETVLLWPQNPQAQGYFWHRLRCVLGRDIQASYDRKHCRGVPTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBS7</accession>
    <name>W5NBS7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transcription factor binding to IGHM enhancer 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006482">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBS7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018118.1">
        <property type="protein sequence ID" value="ENSLOCP00000018086.1"/>
        <property type="gene ID" value="ENSLOCG00000014691.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687259"/>
    <dbReference type="KEGG" id="loc:102687259"/>
    <dbReference type="CTD" id="114834"/>
    <dbReference type="eggNOG" id="KOG1318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157503"/>
    <dbReference type="HOGENOM" id="CLU_031638_1_1_1"/>
    <dbReference type="InParanoid" id="W5NBS7"/>
    <dbReference type="OMA" id="HEHACHS"/>
    <dbReference type="OrthoDB" id="6242697at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014691">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18926">
        <property type="entry name" value="bHLHzip_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="351"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="590" mass="62534" checksum="18CE4B5F9E7180A6" modified="2014-04-16" version="1">MSAVAPDQGKRSQGILLLSPALQLHPDADSIGVDGGDRGVREQVSLPGQLAPHEQPQTVFVILDSSADTVNLISLDSLLPESGIVADIDVENIVASSAETFYQLKSQPVELSPSPPPAGPASSSPGAAMTSRVLLRQQLMREQAREQERREAQQQASAAQLRPSDPSPAISVSAPSPASRPPPAQVPVEVLKVQTNLENPTKYHIQQAQRQQVKQYLSTTLGNKVATQALGVSPSPQPGSAPELTAAASSAPNSPMALLNLGSNSEKEIDDVIDDIISLESSFNDDIMSFIDTSLQMPNTLPVSGNLLDVYSGPGVAAPAITVSNSCPADLPNIKKEMTEAEAKALMKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRAREIESRQKKLEHANRSLLLRIQELEMQARLHGLATPASSIGTEPAVSLLQPQQKQGGQQLEPSSGDALSASLLPASSLGGGAALPFPSSYISPPPSTSPGGVAMNSPHDLGSLSFAELDDSAAAFNSALMSDVGLGDILMDDGGALSPVGAADPLLCSVSPGASKSSSRRSSFSMEEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCD2</accession>
    <name>W5NCD2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2 10">Belongs to the TAF1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEL7</accession>
    <name>W5NEL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TraB domain containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5NEL7"/>
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        <property type="protein sequence ID" value="ENSLOCP00000019076.1"/>
        <property type="gene ID" value="ENSLOCG00000015501.1"/>
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    <dbReference type="HOGENOM" id="CLU_034593_0_1_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
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        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
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        <property type="entry name" value="TraB_PrgY-like"/>
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    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019076.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="39837" checksum="AA440589BC7B161B" modified="2014-04-16" version="1">MDQSNNTEEEPASPDPDDEMHALPSLPPGLSDTEAVELLWQVRAQRRQASPVLPETVTQLQAPDGSVVYLVGTAHFSDSSKRDVAMVRQDLETTQSVIEKEGWSLMLRLTISDSLYSVSNLRLKVNPSSSLQGNHSLSADIKTLILTLALNLTQGSFIGSLRLLMNNSLIQASRVPFCKFHLGDRPIPVTFKRAIAALSLWQKLKLAWGLCFLSDPISKEDVEKCKQKDLLEQTMAEMIGEFPDLHRTIVAERDVYLTYTLRQAARSVEAPPNAQRVPAVVVGVVGIGHVPGIEKNWEKPLNIQEIMSVAPPSRLSRALRTVLKGAVLGALGYAFYRAGGGVGRMLLSLPAVQSLLRPLRPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF02</accession>
    <name>W5NF02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 23">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 23">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 23"/>
    <dbReference type="EMBL" id="AHAT01031398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NF02">
        <property type="interactions" value="1004"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019211"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019243.1">
        <property type="protein sequence ID" value="ENSLOCP00000019211.1"/>
        <property type="gene ID" value="ENSLOCG00000015602.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156962"/>
    <dbReference type="HOGENOM" id="CLU_001232_1_0_1"/>
    <dbReference type="InParanoid" id="W5NF02"/>
    <dbReference type="OMA" id="ECIIEPI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015602">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070776">
        <property type="term" value="C:MOZ/MORF histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:chromatin binding"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001502">
        <property type="term" value="P:cartilage condensation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010172">
        <property type="term" value="P:embryonic body morphogenesis"/>
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    </dbReference>
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        <property type="term" value="P:embryonic pattern specification"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014036">
        <property type="term" value="P:neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15618">
        <property type="entry name" value="PHD1_MOZ_MORF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15527">
        <property type="entry name" value="PHD2_KAT6A_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000123">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000132">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000035">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048589">
        <property type="entry name" value="SAMD1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF26">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21524">
        <property type="entry name" value="SAMD1_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52014">
        <property type="entry name" value="SAMD1_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18 23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAMD1-like winged helix (WH)" evidence="28">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="26">
        <location>
            <begin position="96"/>
            <end position="174"/>
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    </feature>
    <feature type="domain" description="PHD-type" evidence="25">
        <location>
            <begin position="210"/>
            <end position="269"/>
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    </feature>
    <feature type="domain" description="PHD-type" evidence="25">
        <location>
            <begin position="266"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="MYST-type HAT" evidence="27">
        <location>
            <begin position="510"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="330"/>
            <end position="409"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="422"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="792"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="880"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1630"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1790"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1933"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="2018"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="2088"/>
            <end position="2110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="452"/>
            <end position="476"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="822"/>
            <end position="833"/>
        </location>
    </feature>
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        <location>
            <begin position="883"/>
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    </feature>
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            <begin position="902"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="24">
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            <begin position="960"/>
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            <begin position="1006"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="24">
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            <begin position="1033"/>
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            <begin position="1051"/>
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            <begin position="1063"/>
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            <begin position="1167"/>
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            <begin position="1208"/>
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            <begin position="1224"/>
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            <begin position="1544"/>
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            <begin position="1652"/>
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            <begin position="1672"/>
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            <begin position="2095"/>
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    <sequence length="2225" mass="244631" checksum="0DD8741280328DB9" modified="2014-04-16" version="1">MVKLANPLYTDWILEAIKKVKKQKQRPSEERICNAVSMSHGLDRKTVLEQLELSVKDGSILKVSNKGLNSYKDPDNPGRLAFPKPRNNSASPPKASGFDWNKLIKRALEGLQEPGGSSLKSIERYLKGQRDVAASINSAGSSAALFHQQLRLAVKRALAHGRLLKQGPMYQLSGRGVASQTEVPPVDSLASLPPVRLLPHEKDKPVAEPIPICSFCLGTKEQNRDKKPEELISCADCGNSGHPSCLKFSPELTVRVKALWWQCIECKTCSSCQDQGKNADNMLFCDSCDRGFHMECCDPPLTRMPKGMWICQICRPRKKGRRLLHEKAAQIKRRYNTPLGRPKNRSKRPFKKLRGPPGRGGRRRRAGRLSRGSSSPPSSPASSSEGYPGDDRLLFGFSGDGDESPGLRFNKKTKGLIDALTKFFTPSPDGRKARTEVVDYSQQYRARKKGGRKGEAADRTDNQESREEWREGEERPPGMQDSVSEKDMELFRHIQELALQKVGVTGPPDPQVRCPSVIEFGKYEIQTWYSSPYPQEYSRLPKLYLCEFCLRYMKSRSILFQHMRKCGWFHPPANEIYRKDNVSVFEVDGNVSTIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTQNDAKGCHLVGYFSKEKHCQQKYNVSCIMILPQYQRKGYGRFLIDFSYLLSKREGQPGSPEKPLSDLGRLSYMAYWRSVVLETLHQLHDPQITIRRLSKITGICPQDITATLHHLNMLEYRRDRFVLVRREKMIAAHVARLRMNPRQLEVDPDCLRWTPVIVTNTMVSEGEEEEEEEEEEPCKENKPNLTPAPVHWRHGDEEEEDRKCFPALTNSQPSPAASPLHSAQLLADTPLAPANGERRCGRRGRKKQDWLWVKVKDGSRGERGRPRKVRREEEEDEDEEEEGAEEDETPAPPLSPPALLATETELELDPSSRLAEGDQLTRPPPIMPAPRRGRPARKKRGPRKRRLSDGSGEPQPPQTLRLGEAPAPARRGHFSDSSEEEEDDEEGLRPRSPPVLTKPSLGLKCKKTLRKRRVRQRSHPHSSVVTETISETTEVLDEPFEDSDVERPMPRLEPTFEALEEEVAPYSRGQRRYLPPRAALRHSTPAPKRKCHPTVHTESEDEDSTPVLKPAVALHAQDPGAGPPVLGEEDMMEAEPVKKKKKGWPKGKSRKPLHWKKRPGRKPGSTVSRENSEQPPPKLKLKPGRKPRSYYLQRAQEEAARQEQERKRLVQQQLLEKQKLEERAGRKSTRADEGEEEEEEEEDYDDEHKEKPKRQGRPALGTLLGVPHKHTATEEEEEEEDDDDDDDDEDEAWEENHSSSHAIPPPSSHARAPQGRADGEREEEERGSNKKAAVTTASTAGPTKSIGRRSEDHDADDEGDGHLEGKCEKNKNEKRKSQDSEEEDEEEEDEEDEEEEEPASPVRTPPVKEEPQTFLDTVGSAVREGADKQQEEEEEEEEEEEEEELEEEERRRREQEESAAAAAAVEAVTAIPVPEEPIDLQPLHEEPKPVLLIESEHQHPQSHSHTDFKEELSHHHVHHPHSHSHAPAHPHPHHASELDLETVQAVQSLTQGEAQDCEEPEPPGAYQDCEETLAACRTLQSYNHRQEGEDEAAALALVEDCGPASQHSSPLSGHAHHAAPSLPGQSVRSVSSPGLPPGTLEAGAGGGPVTGAGGPGGGGGYTQITPEHPGSLSAPSLQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYDNPSSYDSTMGGGGGGGGGGGGNGNSIAAVAVVAATASSTTSSAAVSSASSSSGSAGSAPPSSSSSSQIGALGSSCSLIQQGGGGGAGGGGNSGGSGGSNVPQPPPPPPPPSASTPGCGIKSPQSCVIERPPSSNQQPQKKVAPQQATPPQPTAPPPPPPPQQQQPQQQQQALSQCSMGNTFASTPMIMEIPESGGGGSGSGGGRSLYDRMGQDFGAGSYPQPSATFSLAKLQQLTNTIMDPHAGMPYSHSAAVTSYATSVSLSNAPPGLAQLAPSPHPPLSQTQPTMTPPPNLGSSSMNLGSPLLQCNMPGTNIGIPPPPHTQRLQGQMATVKTHLSIRSKSAPLPSGSPHQQQLYSRSSGAVTMQGTPRALAVQRGMMPNLMPTPTAYNSMNMNPLNAMPTGYRMPQPMMNSGYHGNPPYMNQPAQYPMQMQMGMMGGQAYPQQPMQPNHHGNMMYTGPSHHTYMNAGVPKQSPYMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF53</accession>
    <name>W5NF53_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat and SOCS box containing 13a, tandem duplicate 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF53"/>
    <dbReference type="FunCoup" id="W5NF53">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019294.1">
        <property type="protein sequence ID" value="ENSLOCP00000019262.1"/>
        <property type="gene ID" value="ENSLOCG00000015647.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160326"/>
    <dbReference type="HOGENOM" id="CLU_000134_4_2_1"/>
    <dbReference type="InParanoid" id="W5NF53"/>
    <dbReference type="OMA" id="AGFWVER"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015647">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000016">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF53">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="21"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="232"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="281" mass="30170" checksum="9E5511E1FB620224" modified="2014-04-16" version="1">KNALMETSTARVNIGDIGFWADRTAVHEAASHGQVLQLKQLIESGACVNVVTVDSITPLHEACLQGQTHCVRLLLAAGAQVDARNIDGSTPLCDACAAGSIECVKLLLEHGAKVNPALFTASPLHEACMSGNSDCVRLMISEGAKLEAHDCHFGTPLHAACAREHVDCVKVLLNAGAKVNAAKLHETALHHAAKVKNVDLIEMLVEFGGNVYARDNQGKKPIDYTRAGSPPLLCLQFYERNPLSLQQLSRVSLRKALGTTGLEVVAQLNIPQRIINYLSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG28</accession>
    <name>W5NG28_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Microtubule-associated protein 1 light chain 3 gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG28"/>
    <dbReference type="FunCoup" id="W5NG28">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019587"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019619.1">
        <property type="protein sequence ID" value="ENSLOCP00000019587.1"/>
        <property type="gene ID" value="ENSLOCG00000015907.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166218"/>
    <dbReference type="HOGENOM" id="CLU_119276_1_1_1"/>
    <dbReference type="InParanoid" id="W5NG28"/>
    <dbReference type="OMA" id="EMFGCFL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015907">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17236">
        <property type="entry name" value="Ubl_ATG8_MAP1LC3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000149">
        <property type="entry name" value="microtubule-associated proteins 1A/1B light chain 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027731">
        <property type="entry name" value="MAP1A/MAP1B_LC3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="141" mass="16153" checksum="807DE68C19C6BDA7" modified="2014-04-16" version="1">KMPPSEKSQHAKPFKQRKSLATRKEEVAGIRLKFPTKIPVIVERYHREKYLPSLDKTKFLVPQELTMTQFITIIRNRMSLTPSQAFYLLVNNSSLASMSLTMAEVYKDNKDEDGFLYMTYASQEMFGSCTECTAVELSKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG76</accession>
    <name>W5NG76_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Elastin microfibril interfacer 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG76"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019635"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019667.1">
        <property type="protein sequence ID" value="ENSLOCP00000019635.1"/>
        <property type="gene ID" value="ENSLOCG00000015947.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RIZH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234633"/>
    <dbReference type="HOGENOM" id="CLU_319805_0_0_1"/>
    <dbReference type="InParanoid" id="W5NG76"/>
    <dbReference type="OMA" id="VESCADI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015947">
        <property type="expression patterns" value="Expressed in larva and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000010">
        <property type="entry name" value="EMILIN-1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF1">
        <property type="entry name" value="EMILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07546">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867717" evidence="9">
        <location>
            <begin position="22"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="11">
        <location>
            <begin position="54"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="10">
        <location>
            <begin position="823"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="131"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="774"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="383"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="525"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="969" mass="107457" checksum="D50AD362640577FC" modified="2014-04-16" version="1">MAGSVPCLLLSFFTLFGGAWSVSYPQRYNLYTGQNQFQSTQSQSQNGPRAASRHRNWCAYVATRTVSCVVEDGVETYVKPDYQPCSWGQIQCSRVVTYRTYMRPRYKVAYKMVTEMEWKCCHGFSGDDCSEGPSGGQSHVTQISTARPRPKRPGQTGSNSGTVQIGGEGRGDTDKVKQLEDKIQSLTKDLHDLQSTLRGLNEKFQEEIRKPGLNGNTHFDAADSEMKDTIDVIQTKLDQLDNRTQAHDEKLVIINNHLANGKGTGNELDTTDIRVQKKFTELKSEILKDLEQRIKLCSTCQSGIEDLRRKQQEDHERIQRLEKQISSMDQRNRQTFESVQENLSRSQSCCSQVTDLRTKVSDIETKMDSISETYIVNGHLDNKNDIRSYVEDLEERLNNTEKRAEEQCASVENDLGLKDSVYANEEHLEELENLISNLNDSLASAVNSCDKTCTSSSGSGTGDEVVDTVKTLEWKVIENTGEIRKFNDRIKDLSVSGDSLFDKVVGISHEIRKIKALTGENGEHFNRIVNDIEDLENTLEVTSKNCDVCSTLQEEVGKLKEEVQKCSGQCKIILNKPTGDEQKPLDGYSVSGPSNANFNSIQGELSGVILTFSSINDTLKGLEHTIQTHSSVIYDLSNTKDKIISEIDKIQQEVSEHIEDSKGRFDHVKMGDCKQSSNALEKRITKMENLCGKLDSVSDSLQKIKDGLNKHVSSLWNCVNKLNTTVTSHSAQFDIIQSTELDGINRKLKILNSSMLYIFNEFNNFTSQDFIGLPGPPGPKGERGLQGVQGPPGREGPVGRTGPEGQRGPPGLRGEQGLPGVDARPPRVSFSAGLTHNLVNSGTIVFDKILVNDGGFYDPTTGIFTAPYDGRYYFSAILTGHKNEKIEAVLSKSNYGILRVDSAGYQPEGLENKPVAENKPTPGSLAVFNIILPLNIGDTVCIDLVMGKLADSVEPLTIFSGMLLYEDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGB0</accession>
    <name>W5NGB0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01001622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638784.2">
        <property type="nucleotide sequence ID" value="XM_006638721.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGB0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019669"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019701.1">
        <property type="protein sequence ID" value="ENSLOCP00000019669.1"/>
        <property type="gene ID" value="ENSLOCG00000015971.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693520"/>
    <dbReference type="KEGG" id="loc:102693520"/>
    <dbReference type="CTD" id="55304"/>
    <dbReference type="eggNOG" id="KOG1357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158513"/>
    <dbReference type="InParanoid" id="W5NGB0"/>
    <dbReference type="OMA" id="MFGSNAY"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015971">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000004">
        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000047">
        <property type="entry name" value="serine palmitoyltransferase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF56">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="18">
        <location>
            <begin position="163"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="548" mass="61493" checksum="D05BACDA861AC42F" modified="2014-04-16" version="1">MSMASTANIVRNGNVKFNLNQNNIKNDSITRNGFSKNCHQKNGTANGDLQKWKYKESFEQAPLYVAVLTYLGFGVVTLFGYFRDFLRAIGLEKCHIAQEREEQKDFVPLYQDFENFYTRNLYMRVRDNWNRPICSLPGPVFDLMERVTDDYNWTFRHTGKAIKNVINMGSYNYLGFAENNFLETVADTTKEYGVGVCSTRQEMGNLDIHEELEDLVATFLGVESAMTFGMGFATNSMNIPALVGKGCLILSDELNHTSLILGARLSGATIRVFKHNNMQSLEKLLRDAICSGQPRSHRPWKKILILVEGIYSMEGSIVRLPDIIALKNKYKAYLYLDEAHSIGAVGPTGRGVVELFGLDPSEIDVLMGTFTKSFGAAGGYIAGKKDLVDYLRTHSHSSVYASAMSPPVAKQIIRAMKCIMGLDGTTVGIQRVQQLAENTRYFRKKLHEMGFIIYGNEHSPVVPLLLYMPGKVGAFARKMLKRGIGVVVVGFPATPITESRARFCVSAAHTREMLDKVLNTLDELGDYLTLKYSRKTPPSKSELYDERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH92</accession>
    <name>W5NH92_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="36">Potassium channel subfamily K member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NH4(+)(in) = NH4(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28747"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="35">Homodimer; disulfide-linked. Heterodimer with KCNK2; disulfide-linked. In astrocytes, forms mostly heterodimeric potassium channels with KCNK2, with only a minor proportion of functional channels containing homodimeric KCNK1. Interacts with KCNK3 and KCNK9, forming functional heterodimeric channels. Interacts with GNG4. Identified in a complex with PSD and ARF6; interacts only with PSD that is bound to ARF6. Interacts with UBE2I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 38">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638829.1">
        <property type="nucleotide sequence ID" value="XM_006638766.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH92"/>
    <dbReference type="FunCoup" id="W5NH92">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020001"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020034.1">
        <property type="protein sequence ID" value="ENSLOCP00000020001.1"/>
        <property type="gene ID" value="ENSLOCG00000016222.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685558"/>
    <dbReference type="KEGG" id="loc:102685558"/>
    <dbReference type="CTD" id="561206"/>
    <dbReference type="eggNOG" id="KOG1418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155293"/>
    <dbReference type="HOGENOM" id="CLU_022504_6_0_1"/>
    <dbReference type="InParanoid" id="W5NH92"/>
    <dbReference type="OMA" id="SAWCFGL"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016222">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="F:outward rectifier potassium channel activity"/>
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    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
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        <property type="term" value="P:heart process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:potassium ion transmembrane transport"/>
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        <property type="entry name" value="Potassium channel subfamily K member"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="2pore_dom_K_chnl"/>
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        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
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        <property type="entry name" value="2pore_dom_K_chnl_TWIK"/>
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        <property type="entry name" value="2pore_dom_K_chnl_TWIK1"/>
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        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 1"/>
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        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
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        <property type="entry name" value="Ion_trans_2"/>
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        <property type="entry name" value="K_channel_subfamily_K_type"/>
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        <property type="entry name" value="2POREKCHANEL"/>
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        <property type="entry name" value="TWIK1CHANNEL"/>
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        <property type="entry name" value="TWIKCHANNEL"/>
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    <keyword evidence="20 36 38" id="KW-0406">Ion transport</keyword>
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            <begin position="21"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
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    </evidence>
    <sequence length="337" mass="38498" checksum="EC2D2978CD2F5654" modified="2014-04-16" version="1">MLQSLTSNSCVRLIQSNKSTWYFLFLILGYVLYLIFGAVVFSSVELPYEDELRQDLRNVKRKFLQENECLSEDRLEEFLAKALEASNYGVSMLNNATANWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRIMVFSTRRPVTYIHTHWGLSKPLVGMIHAIFLAFITASCFFIIPAVVFSALEENWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKLGITVYLLLGLIAMLVVLETFCELQQLKQFRKMFYLKREKPEDRVTILDHDHLSFSSVSDQAATLREEKITEPFVDVQPVLYTATDGSINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHE4</accession>
    <name>W5NHE4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain containing 107</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHE4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020053"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020086.1">
        <property type="protein sequence ID" value="ENSLOCP00000020053.1"/>
        <property type="gene ID" value="ENSLOCG00000016259.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695206"/>
    <dbReference type="KEGG" id="loc:102695206"/>
    <dbReference type="eggNOG" id="ENOG502QQ24">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000034107"/>
    <dbReference type="HOGENOM" id="CLU_079920_0_0_1"/>
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    <dbReference type="OMA" id="WEHSELI"/>
    <dbReference type="OrthoDB" id="9904035at2759"/>
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        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016259">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="P:protein localization to cell surface"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
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    </dbReference>
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        <property type="entry name" value="Ric3"/>
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        <property type="entry name" value="RIC3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723:SF2">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
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        <property type="entry name" value="RIC3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="303" mass="33892" checksum="F5C4DA47469D91A2" modified="2014-04-16" version="1">MVLTYSQQLTVAFAAVMCGCLVFPRMFGGGTGSKDPRAFEPHFSRKVGPGPLRGHQVNMDTGSGKGQSFENIQQMRKMMEQEIKSERLKANNKGYVFTLMPLYAIGVGVFAVYKFLKIKSSESQTGKEKTSKGAKKSEETENQLNELEQRLAQTEKMLNSILTQLDPLTTCVESVAFEQKNEIMAQLHSIRQLMKKRGMDFPPLNPEEPPCGKNLEDILQSMTAQEEETEGRTVDKQDVLEHQDPDTSLGAALESEEDKNSGSDHHMASLESSFETNIENVGAININSEEVEETGLRRRNRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHM8</accession>
    <name>W5NHM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Si:ch211-93n23.7</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHM8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020170.1">
        <property type="protein sequence ID" value="ENSLOCP00000020137.1"/>
        <property type="gene ID" value="ENSLOCG00000016315.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167263"/>
    <dbReference type="HOGENOM" id="CLU_085743_0_0_1"/>
    <dbReference type="InParanoid" id="W5NHM8"/>
    <dbReference type="OMA" id="PCHYNVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016315">
        <property type="expression patterns" value="Expressed in embryo and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001939">
        <property type="term" value="C:female pronucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042585">
        <property type="term" value="C:germinal vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001940">
        <property type="term" value="C:male pronucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044727">
        <property type="term" value="P:epigenetic programing of male pronucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678:SF1">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="207" mass="23660" checksum="724F3684725A7ED7" modified="2014-04-16" version="1">ERDGWWRSEIKDSPNPGSYHIKDFIEESRLNPVKITYSFKGSGRKYPVGVVRKGDFLLPGAYSLTNFVQQAEKRNATYCFKNSPRPNNFTLGIRDKDIDVSPCHYNVTEKPVVKLPCNHVMFRSAVQRLTFIPKEGPAPGQYHVTKQSGQHAITSCFRSTVPRLVTPRSKTPGPGTYEPLWQTTWLLKRGSATERVHGLFFRNMLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI93</accession>
    <name>W5NI93_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Extracellular tyrosine-protein kinase PKDCC</fullName>
            <ecNumber evidence="3">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Protein kinase domain-containing protein, cytoplasmic</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Protein kinase-like protein SgK493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Sugen kinase 493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Vertebrate lonesome kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:10597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01001698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218732.1">
        <property type="nucleotide sequence ID" value="XM_015363246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI93"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020352"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020386.1">
        <property type="protein sequence ID" value="ENSLOCP00000020352.1"/>
        <property type="gene ID" value="ENSLOCG00000016475.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692235"/>
    <dbReference type="KEGG" id="loc:102692235"/>
    <dbReference type="CTD" id="559418"/>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001205"/>
    <dbReference type="HOGENOM" id="CLU_044025_1_0_1"/>
    <dbReference type="InParanoid" id="W5NI93"/>
    <dbReference type="OMA" id="HEGCLLS"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016475">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000552">
        <property type="entry name" value="extracellular tyrosine-protein kinase PKDCC isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042983">
        <property type="entry name" value="PKDCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448:SF3">
        <property type="entry name" value="EXTRACELLULAR TYROSINE-PROTEIN KINASE PKDCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="183"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="538" mass="60898" checksum="7A044A02422B39FE" modified="2014-04-16" version="1">MKRRKIAVAAGFCFSFFLGTLMNLLFIPGFDRHRNNSNRVQRYYQEQQHVAGSRDLNLRDPAAPPGAYMNREPTASSQHRDLLQQIQERYDEVLRYRQNWPPPPGSGNPVRTLERRRLMDLAPRSSSEQSRATERLNNRYTEPHHRGSSSSASLYARTQVDFDLKMNSVNSRQMLGCSSIDSATNVQYLGSGYTKAVYKVALNESFSVALKSVDFGGHDMENCVKKYASPGNCYRLASYKIIKEMTLLQRLQHPNILKLYGYCYHDSNDISDTLTAITELGSPLEMIQLLQTSWEERFRICLSLVRLLHYLANSPLGSVTLLDFRPRQFVLVDGELKVTDLDDASAEEAPCSSSADCFMEFPARNFTLPCSSQGRCLAINEKRNLYNAYRFFFTYLLPHSAPAALRPLLDKIVNSTGELIWGINETLGHLEKVLHLYRNGLYLQNMTHTPRAEYKRVIDATIAGDNYRCWPSYQHEGCLLSVFSVREAIGVCESHAQCRAFVMTNQTTWTGHQLVSFKTGSASLVAAPGKVAFLKATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIF4</accession>
    <name>W5NIF4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box transcription factor T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Brachyury protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein T</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIF4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020413"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020448.1">
        <property type="protein sequence ID" value="ENSLOCP00000020413.1"/>
        <property type="gene ID" value="ENSLOCG00000016526.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157912"/>
    <dbReference type="HOGENOM" id="CLU_038303_1_1_1"/>
    <dbReference type="InParanoid" id="W5NIF4"/>
    <dbReference type="OMA" id="TGAGECP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016526">
        <property type="expression patterns" value="Expressed in embryo and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20192">
        <property type="entry name" value="T-box_TBXT_TBX19-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000002">
        <property type="entry name" value="T-box transcription factor Brachyury"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF83">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="13">
        <location>
            <begin position="47"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="390"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="436" mass="47714" checksum="D265E0C458D5B732" modified="2014-04-16" version="1">TMTSSSTESAGKNLQYRVDHLLSAVEHELQAGSEKGDPTERDLKVSLDENELWLKFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHSFPETQFIAVTAYQNEEITSLKIKYNPFAKAFLDAKERSDHKDILDDVGDNQQSGYSQIGGWFIPGTGSLCPPANPHPQFGNPLTLSSPHGCDRYSTLRNHRSAPYPSPYAHRNNSPTGYTDNSSACLSMLPAHDNWSGLQVPTHSSMLPMAHSTSPSTSSSQYPSLWSVSNSTITPVSQPGNMSNGLGSQFLRGSTAHYPSLSHPVAAPSAGSPLYDSAAPAEVHDPQYDTSPHARLPPSWASVTPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJR5</accession>
    <name>W5NJR5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cilia and flagella associated protein 61</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJR5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020874"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020910.1">
        <property type="protein sequence ID" value="ENSLOCP00000020874.1"/>
        <property type="gene ID" value="ENSLOCG00000016887.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSEC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004987"/>
    <dbReference type="InParanoid" id="W5NJR5"/>
    <dbReference type="OMA" id="RWNEGQI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016887">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120316">
        <property type="term" value="P:sperm flagellum assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038884">
        <property type="entry name" value="CFAP61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056299">
        <property type="entry name" value="CFAP61_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032151">
        <property type="entry name" value="CFAP61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21178:SF8">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23150">
        <property type="entry name" value="CFAP61_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16092">
        <property type="entry name" value="CFAP61_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 61 N-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Cilia- and flagella-associated protein 61 N-terminal" evidence="1">
        <location>
            <begin position="385"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP61 dimerisation" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1239" mass="140577" checksum="13882251F0562167" modified="2014-04-16" version="1">MRTITSSGGKEEIVTARRAESSDAPEISNLILPSTKDVFGRLNVIYLLEKANLAVTLNSANNEVIAHAAFLDYPNLDLVDQACWEPWLHNNYNIDKCTPLNTLFMHLYVARPDFCIGSSKEIVRTVFNAVTELHYIFLFAPVGSTLEPALAAVFEPVETLQTSNTDYMAFVCHRHRHCPVLHIRRARVEDHDDLIPIFAQQTDKLGSTYGDYFLAELIEAQDEDNHAAVCESEGTAVGFISLSGDINVKLLNECFELGPYHGLHKPHPDDILDQPSEPRWESGIKMILIKQSCILMVEETKSVIPQDTSTNCLQDSDLKVHTVEPFNVYQIATDTKQADVLDLSSADRDSRRESVVSDASESHLLLYYSPTENREKVRTVVYKGALNAFCIQLFVIDEKYEMRSVDFLPYIFKLYPDRAFCVITVPKLAPEFPLLQSFVRAVPYHTSTLSQELYIFHRSGLLKKFCVRLARSSDKTGVESLVNKLSLQDAVLEDLDMYCQARRDPDGTPVQAFVAEVLDQIVGILIIRNEEDIDFIRSHYNIENFIYFSHHTMEEHGQLLHFALNPIFQHYAKHFFKEALRLSHKSSLYHRVYPSHEDVKFSQNAYAHSLTSALNCMVPVRPRRQIVYPLDELGIDAPSRRISKDQVPYALNHINRKLTLEPKVTINARIVVVGASDTGISFLEVLAYCPHLQFNNITLISTHGLPGGCSGYSENKTFLATSYCYSDKDYALLSLRSWINVVVGKMQGIDRAAKHVLVSDDRKVPYDHLILCTGQQYQVPCPTGIDISQLSTNRDIAIRTGERYTGPVPSNLFTLNDDRDCLKTYNWLQHNFVHSEGNAVLFGSTIDVYTTLETMLSLGISASRIHLVQPPLDDTVSCFNNFEVERAVEEAMRRAGVTLHHNCVLAQWNDGEDTEPITSVSFTTDTKPFKLPCSVFINFYKKRIDYDAFKAINDAFLVYDGRLVIDTTFHTSDPTIRAAGPLTKFARRYHADQWTHSNFNSKEVGFQLAAAILPFFDPTLEWVADPPVEFDRLIPMFRGAKVQGGKLPGGYHYLHASKPGIGTPLAAQMSQSKYGREIVTGSSENGNYFRLHLNQYNEVETITCLSLKPFPASNYLCLYGENEQLLNGLCDRFDEGLISDFYSYFKESWCLAIYHDRFMDFKQEVLQIMASRQNPDSPSFAEIAQQIVDEELSLPEEPKQYLRRTAEECNIRKPVERSTLSYLKYNRCHLPMYALPGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMZ1</accession>
    <name>W5NMZ1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial transcription termination factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMZ1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022000"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022039.1">
        <property type="protein sequence ID" value="ENSLOCP00000022000.1"/>
        <property type="gene ID" value="ENSLOCG00000017897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694751"/>
    <dbReference type="KEGG" id="loc:102694751"/>
    <dbReference type="CTD" id="80298"/>
    <dbReference type="eggNOG" id="KOG1267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063817"/>
    <dbReference type="HOGENOM" id="CLU_058644_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMZ1"/>
    <dbReference type="OMA" id="PEAVLCN"/>
    <dbReference type="OrthoDB" id="9868878at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017897">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006393">
        <property type="term" value="P:termination of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000068">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000042">
        <property type="entry name" value="Mitochondrial transcription termination factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437:SF1">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="378" mass="42910" checksum="108F03624FAAE0B4" modified="2014-04-16" version="1">MFRLAVAPFWVHCPRVRACLSGSRSYAVSGGGNERAENKLTVNSLYSLSVDIRKIRKLKGWVLLQDIAYVDETASVLKELGAEATLIATVLEQCPEAVLCTPAEVRAQRELWMSVCPTEKELLRIIEKFPHSFFTASQHINQKANIQFFQRLKINKRTIIKLMASASQSFSNPVEQNEQMISTLQDMYRSLGGKEGNLQIWLQKLLTQNPFILMKAPETLKENLKFLQKKGFSSEELLLLLSKLRGFIAELSPCRMEETLTCSKAVLRCTEWELKEIVLKCPALLYYSVPVLSDRLQGLLSRGISVDQIKESPSVLELTTQIVQYRIQKLSASGYDIKKGSLEPLNGTNKDFELSYEKLHLRKQRPLFNPVAPLTIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN24</accession>
    <name>W5NN24_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN24"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022072.1">
        <property type="protein sequence ID" value="ENSLOCP00000022033.1"/>
        <property type="gene ID" value="ENSLOCG00000017930.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102693879"/>
    <dbReference type="eggNOG" id="ENOG502QW4X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NN24"/>
    <dbReference type="OMA" id="SIMSIIW"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017930">
        <property type="expression patterns" value="Expressed in bone element"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="49"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="37065" checksum="A1A098135DD9666A" modified="2014-04-16" version="1">MTSVITTSPVLNTTFIRPNGFYIRGFYALENSNYFFIFLGVIYVATLLANASIMSIIWFAQPLHTPKYFGVFSLALVDVSYSTSLIPKSLDLFLYGNRLVEYNTCLTQMFFVHYFSAMESYALMVLAYDRFIAICFPLRCKTINSNTKMIIVIIISWVIPFIVVLTMVSFITRLSYCKSVIVNSYFCDHGPVFKISCSDYSINWFMAAFFIVSLFFLPLALILLSYVCILVSLLKIAEADGRRKAFRTCTSHLILVAVFYVPLLATYIIAWVNVYIDTDTRILNTSLSAAIPPLLNPIIYTLKTDEIMDQIKKYIRNRKMALSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP10</accession>
    <name>W5NP10_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NP10"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022369"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022410.1">
        <property type="protein sequence ID" value="ENSLOCP00000022369.1"/>
        <property type="gene ID" value="ENSLOCG00000018268.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVH7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282520"/>
    <dbReference type="HOGENOM" id="CLU_012526_1_0_1"/>
    <dbReference type="InParanoid" id="W5NP10"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="33332" checksum="D5A8E6BD17B9C16F" modified="2014-04-16" version="1">EYNSALFIFFLILLLATFLGNLLIVVLISLDQRLHTPMYFFLWNLAVLDVLITVTVIPKLLVVLLGHKRTISFSDCFAQMYFFTTFAVVEVFLVAVMAYDRYVAIVKPLHYNNIMSSKVCITMMSSAWVLGFLVPLTPVLLASTLPFCGSNLIQHIVCDYTTVMSLACGDVTAQVSFALCLAMVVIYIPVLFVLWSYYRIICSVIKMKTLESRKKAFSMCSSHVILVFLYYMSSAVVYIGLKVESIPTDGRIFIGAVHYFLTPFINPIIYSLSNEKIKAAAHRYLKLHTSFQHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP27</accession>
    <name>W5NP27_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP27"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022386"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022427.1">
        <property type="protein sequence ID" value="ENSLOCP00000022386.1"/>
        <property type="gene ID" value="ENSLOCG00000018285.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286936"/>
    <dbReference type="HOGENOM" id="CLU_002678_42_11_1"/>
    <dbReference type="InParanoid" id="W5NP27"/>
    <dbReference type="OMA" id="GLARHMH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018285">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001804">
        <property type="entry name" value="E4F transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000736">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="110" mass="12405" checksum="15F60B76108F640E" modified="2014-04-16" version="1">TGEQPYHCTVCGKTFSISETLKRHLRIHTGERPFSCATCGKGFRESGSLRKHQRIHTGEAPYRCSECGQSFRESGKLKIHQRIHTGETPYHCTVCGKSFSLSGTLKIHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP64</accession>
    <name>W5NP64_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2</fullName>
            <ecNumber evidence="4">2.4.1.312</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Extracellular O-linked N-acetylglucosamine transferase-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glycosyltransferase-like domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37663"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:13618"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="ChEBI" id="CHEBI:137540"/>
            <dbReference type="EC" id="2.4.1.312"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 61 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.312" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01012335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635585.1">
        <property type="nucleotide sequence ID" value="XM_006635522.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212293.1">
        <property type="nucleotide sequence ID" value="XM_015356807.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212294.1">
        <property type="nucleotide sequence ID" value="XM_015356808.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212296.1">
        <property type="nucleotide sequence ID" value="XM_015356810.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069051358.1">
        <property type="nucleotide sequence ID" value="XM_069195257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP64"/>
    <dbReference type="FunCoup" id="W5NP64">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022423"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022464.1">
        <property type="protein sequence ID" value="ENSLOCP00000022423.1"/>
        <property type="gene ID" value="ENSLOCG00000018322.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697824"/>
    <dbReference type="KEGG" id="loc:102697824"/>
    <dbReference type="CTD" id="84892"/>
    <dbReference type="eggNOG" id="KOG4698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160695"/>
    <dbReference type="HOGENOM" id="CLU_020169_0_0_1"/>
    <dbReference type="InParanoid" id="W5NP64"/>
    <dbReference type="OMA" id="EFQMRVV"/>
    <dbReference type="OrthoDB" id="529273at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018322">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055001">
        <property type="term" value="P:muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001371">
        <property type="entry name" value="Protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF38">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="490"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="586" mass="67719" checksum="0D9737819A0C07B1" modified="2014-04-16" version="1">MRGPGCRMNVAAVLNGLLVSVLAAVLWKYARLREHAAVVEEELQLSRQSQELSQARIDYHAALRALQEDGTRMVCSGKMHTDRLCRFDYLCYSTEAEEFVFFHSNASVMLPNLGSRRFQPALLDLSSVEDHNTQYFNFLELPAAALKFMPKPVFVPDVALILNRFNPDNLMHIFHDDLLPIFYTMSQYSDLDDEARLVFMEGWNEGTHFDLYRLLSNKQPLLKEQLKNFGKLLCFTKSYVGLSKITTWYQYGFVQPQGPKANILVSGTEIRQFTRFFMDKLNITREEVSENEEYIVVFSRTINRLIINEAELILTFAQEFQMKTIVVSLDEHSFADIVQIISRASMLISIHGAQLVTSLFLPRGAVVVELFPYAVNPEHYTPYKTLASLPGMDLQYVAWQNTKEENSVAFPDRPWDQGGIAHLEQEEQERILKSKEVPRHLCCRNPEWLFRIYQDTKVDIPSLLEVLRQTVKSKPALKKAKPASTVHPGRVREPRCQASVQGSNEAKLTVSWQIPWNLKYLKVREVQYEVWIQEQGENTYMPYILPHQNYTFSENIKPFTTYLVWIRCIFNKTLLGPFADVLACRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV44</accession>
    <name>W5LV44_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="23">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="23">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17 23">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D-galactosyl-(1-&gt;4)-N-acetyl-D-glucosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17745"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60152"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="2.4.1.90"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:17746"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP-alpha-D-galactose = a neolactoside nLc4Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31499"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:17103"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.275"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:31500"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a beta-D-glucosylceramide + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1)-ceramide + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:79208"/>
            <dbReference type="ChEBI" id="CHEBI:83264"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:62553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a neolactoside IV(3)-beta-GlcNAc-nLc4Cer + UDP-alpha-D-galactose = a neolactoside nLc6Cer + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:90357"/>
            <dbReference type="ChEBI" id="CHEBI:144378"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:62549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an N-acetyl-beta-D-glucosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61631"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="EC" id="2.4.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:22933"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 23">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 23">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Golgi apparatus membrane</location>
            <topology evidence="23">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="W5LV44"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000001"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000001.1">
        <property type="protein sequence ID" value="ENSLOCP00000000001.1"/>
        <property type="gene ID" value="ENSLOCG00000000001.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158549"/>
    <dbReference type="HOGENOM" id="CLU_044391_1_2_1"/>
    <dbReference type="InParanoid" id="W5LV44"/>
    <dbReference type="OMA" id="CDPGGPR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000001">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003831">
        <property type="term" value="F:beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003945">
        <property type="term" value="F:N-acetyllactosamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006682">
        <property type="term" value="P:galactosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000028">
        <property type="entry name" value="beta-1,4-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF33">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 23" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 23" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 23" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 23" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="25">
        <location>
            <begin position="85"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="24">
        <location>
            <begin position="223"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="368" mass="41529" checksum="793233AB6D6ED29E" modified="2014-04-16" version="1">CMGRSLDSPCTLAILVGFQFAFVVYFSLGGFKGLVSVLVRSSDPEFDYSKPHDVYTNLSQLVPPPNPAETAGIAVATGRGHLKDCPETSPLLVGPVAVVLSSSLSLAQIQERNPLVLPGGRYLPPDCEPRHHTAVIIPYRNRQAHLRALLYHIHPFLQRQQLHYGIYIVHQAGNATFNRAKLLNVGVKEALRDEEWQCLFLHDVDLLPENDHNLYVCDRKSPKHVSVAMDKFRYRLPYPQYFGGVSAVTPDQYLKMNGFPNQYWGWGGEDDDIAARVRLSGMKIIRPSVSVGHYKMIKHRGDKGNEENPRSLRFDLLMRTRRNWNSDGVNSLTYELLSKKLEPLYTNLSVNIGEDPRKPQKKPLDTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWG7</accession>
    <name>W5LWG7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Perforin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWG7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000102.1">
        <property type="protein sequence ID" value="ENSLOCP00000000102.1"/>
        <property type="gene ID" value="ENSLOCG00000000092.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000000474.1">
        <property type="protein sequence ID" value="ENSLOCP00000000474.1"/>
        <property type="gene ID" value="ENSLOCG00000000434.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063725"/>
    <dbReference type="HOGENOM" id="CLU_039516_2_0_1"/>
    <dbReference type="OMA" id="RCERYSP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000434">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF1">
        <property type="entry name" value="PERFORIN 1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014109543" evidence="8">
        <location>
            <begin position="32"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="10">
        <location>
            <begin position="36"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="382"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="524" mass="57732" checksum="E43D1CE3E488465C" modified="2014-04-16" version="1">PPAAMASLTQQRLRPLSLALQVLALLDGHLACRTGWYTACQQAPFVPGYNLAGEGFDIVRMQRKGAYVIDVRTFLTPNQTCTLCENRLQGGQLQKLPLSVLDWRFGSQCRQQLASAVHESYEAVVSSSTSSISNQWEAGLDLDKVGKVALGGTQSQISHFAMSHARADRFVFSSDEFSCKSYRFRLTDRPPLSTDFLRHLQGLPPQYNSSTKAAYQRLIDTYGTHYVRQAELGGWLRRTTAIRSCLATLNGHTASFASECLSAQLALQLGLVGASASSARCRAVLENHDSKMGFSSGFLAHLMEVQGGQQWLREALRSGGGRPSFTDWLRSLTDFPDVVSCALHPLHELVREPRVREALKRAVRSHIQGHALQEGSAAAQSCSGVPNLHSNCCPLEPGWGRLRVTVERASGLRGDPVGQTEGYVKVWFGSRFQQTHFVKENNNPVWNAAYDFGAGHSAQQLVFEVWDKDVQHDDKLGRCTLTPQQGSHSSSCSLAQGGVFYYSYTFTCDPHLTGLSCRTYKPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWY0</accession>
    <name>W5LWY0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWY0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000637"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000639.1">
        <property type="protein sequence ID" value="ENSLOCP00000000637.1"/>
        <property type="gene ID" value="ENSLOCG00000000576.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2628274_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWY0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000576">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="1">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="78" mass="8641" checksum="871801369B6F94EC" modified="2014-04-16" version="1">ADSHNPQFTDIINQKGTEGQITMTQTPTTVSVSPGQDISLRCSTSQDISLKIVWYQLQAGYSPKLLIKTGDERFDELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXR6</accession>
    <name>W5LXR6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">medium-chain acyl-CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a medium-chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48340"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:59558"/>
            <dbReference type="ChEBI" id="CHEBI:90546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48341"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.2" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01032371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXR6"/>
    <dbReference type="FunCoup" id="W5LXR6">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000923"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000927.1">
        <property type="protein sequence ID" value="ENSLOCP00000000923.1"/>
        <property type="gene ID" value="ENSLOCG00000000802.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682727"/>
    <dbReference type="KEGG" id="loc:102682727"/>
    <dbReference type="CTD" id="6296"/>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157930"/>
    <dbReference type="InParanoid" id="W5LXR6"/>
    <dbReference type="OMA" id="HAWSNLF"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000802">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM2A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="15">
        <location>
            <begin position="80"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="16">
        <location>
            <begin position="494"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="588" mass="66031" checksum="613CFEB46E36A351" modified="2014-04-16" version="1">MAKLAFSLKVLKGPGRGCRSILALLGTRHQRRGHLSSVPRNFEDYESLRKIHNPQVPEHFNFAKDVIDKWAEKEKLGGRAESPAFWWVNDGGDQIRWTFEELALHSRRTANVLSGPCDLQRGDRVLLILPRLPEWWLVNVACMRTGTVLIPGTSQLTARDILQRLRVSQAKCIITDESLAPVINAISPECSWLKAKLLVSNKKQDGWMNLTELSKTVSSEHTCMQTRSDDPMTIFFTSGTTGSPKMTQHTHCSYGIGLAVNGRFWLDLTEEDVLWNTSDTGWAKSAWSSVFAPWTQGACVFVHRMAQFHPRTVLETLSKYPISAFCSAPTAYRMLMQEDISRYRFPYLKHCVCAGEPVNPDLLEKWKDHTGLDIYEGYGQTETVLIAGTFKGMKIKPGSLGKPSPAYNVQVVDEMGNIVPRGTEGNIAIKVKPQKPFCLFSGYTDDAVSTALCYRGDFYLTGDRGLMDEDGYLWFFGRADDVILSAGYRIGPFEVENALAEHEAVAESAVVSSPDPVRGEVVKAFVVLTPEYAAHDKQQLAAELQKHVKQVTAPYKYPRKIEFVEQLPKTVSGKIRRLELRNKEWGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYI9</accession>
    <name>W5LYI9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zgc:73226</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015215528.1">
        <property type="nucleotide sequence ID" value="XM_015360042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYI9"/>
    <dbReference type="FunCoup" id="W5LYI9">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001196"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001200.1">
        <property type="protein sequence ID" value="ENSLOCP00000001196.1"/>
        <property type="gene ID" value="ENSLOCG00000001062.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698806"/>
    <dbReference type="KEGG" id="loc:102698806"/>
    <dbReference type="eggNOG" id="ENOG502RYT0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013415"/>
    <dbReference type="HOGENOM" id="CLU_091463_1_0_1"/>
    <dbReference type="InParanoid" id="W5LYI9"/>
    <dbReference type="OMA" id="CVTIQED"/>
    <dbReference type="OrthoDB" id="5857140at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001062">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
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    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF9">
        <property type="entry name" value="BCL-2_ADENOVIRUS E1B 19KD INTERACTION PROTEIN XR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="73"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="19948" checksum="3F46B04AA2B0DB41" modified="2014-04-16" version="1">MTAPRAPSPPDSLLGSWEELQACEAGVEGPARTHSSLLQGELESILLEAQLESERGSRTDSPPQVLSPRNRASPRTESEPGNSTEHPTVQSEEWQCPNTDVDRSGSSQWVWDWASRPENLPPKEFLFQHPKQTGSLSERKRSFFSSDLLFLFIPSILASHLLTLGLGIYIGKRLAASSAGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYS3</accession>
    <name>W5LYS3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="EMBL" id="AHAT01011260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYS3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001280"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001284.1">
        <property type="protein sequence ID" value="ENSLOCP00000001280.1"/>
        <property type="gene ID" value="ENSLOCG00000001129.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT06">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159185"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_2_1"/>
    <dbReference type="InParanoid" id="W5LYS3"/>
    <dbReference type="OMA" id="ACPNTSA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001129">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09938">
        <property type="entry name" value="SH2_N-SH2_Zap70_Syk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.930.10:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000185">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000031">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000216">
        <property type="entry name" value="Tyrosine-protein kinase SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.930.10">
        <property type="entry name" value="Syk Kinase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023420">
        <property type="entry name" value="Kinase_SYK/ZAP-70_inter-SH2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035838">
        <property type="entry name" value="SYK/ZAP-70_N_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012234">
        <property type="entry name" value="Tyr_kinase_non-rcpt_SYK/ZAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000604">
        <property type="entry name" value="TyrPK_SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 19" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="10"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="163"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="329"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="335"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="609" mass="69098" checksum="2D2A5B119FB6D180" modified="2014-04-16" version="1">MPDPAADLPFFYGSISRSEAEEVLKLAGMSDGLFLLRQCLRSLGGYVLSMVCDVQFHHYPVERQLNGTYAIAGGKPHCGPAELCEYYSKDPDGLVCTLRKPCLRPPGVDPKLGVFDSLRENMLREYVRHTWNLEGEAMEQAIISQAPQLEKLIATTAHEKMPWYHGKIPRLEGERRLYSGVQPDGKFLVRDRDESGTFALSMIYGKTAYHYQIIQDKSGKYSMPEGTKFDTVWQLVEYLKLKPDGLVYTLREPCISPNCTIAQAAPVPPSAVSIRKLGANGYTPPPKAPLALGAPQRPTPMDTSVYESPYSDPEELKDKKLFLKREQLMIDEVELGSGNFGCVKKGVYKMRKKQIDVAIKVLKSENEKAVKDEMMKEAEIMHQLDNPYIVRMIGICQAESLMLVMEMAPGGPLNKFLSSRKEQISVENVVELMHQVSLGMLYLEQKNFVHRDLAARNVLLVNQHYAKISDFGLSKALGADDNYYKARTAGKWPLKWYAPECINFHKFSSKSDVWSFGITMWEAFTYGQKPYKKMKGPEVISFIEAGNRMDCPSVCPEEMYTLMKDCWIYKHEERPGFLIVEERMREYYYSISKKTTPATPTEKPPEVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZC6</accession>
    <name>W5LZC6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integrator complex subunit 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01032364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5LZC6">
        <property type="interactions" value="1111"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001483"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001488.1">
        <property type="protein sequence ID" value="ENSLOCP00000001483.1"/>
        <property type="gene ID" value="ENSLOCG00000001281.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015743"/>
    <dbReference type="HOGENOM" id="CLU_001690_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZC6"/>
    <dbReference type="OMA" id="CSEFRFY"/>
    <dbReference type="OrthoDB" id="19938at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001281">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904059">
        <property type="term" value="P:regulation of locomotor rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160060">
        <property type="term" value="P:sleep homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034474">
        <property type="term" value="P:U2 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053964">
        <property type="entry name" value="INT1_R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038902">
        <property type="entry name" value="INTS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053966">
        <property type="entry name" value="INTS1_INTS2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053965">
        <property type="entry name" value="INTS1_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022145">
        <property type="entry name" value="INTS1_RPB2-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21224">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21224:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22927">
        <property type="entry name" value="INT1_R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22929">
        <property type="entry name" value="INTS1_INTS2-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22928">
        <property type="entry name" value="INTS1_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12432">
        <property type="entry name" value="INTS1_RP2B-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 1 RPB2-binding" evidence="2">
        <location>
            <begin position="323"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 INTS2-binding" evidence="5">
        <location>
            <begin position="970"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 R3" evidence="3">
        <location>
            <begin position="1841"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 R4" evidence="4">
        <location>
            <begin position="2058"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="921"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2196" mass="245392" checksum="0692D4290A26EC3A" modified="2014-04-16" version="1">MNRPKPATIRRPSSAAKPSGHPPPGDFIALGSKSQASEPKAPAVLLKPASTSLPSDRKREAASTLPSASGLSGLTKRPKLSSTPPLSALGRLADVASVDKRAISPSIKEPSVVPIEVPPAVLLDEIEAAEAEGNDDRIEGILCGAVKQLKINRAKPDITLYLSLMFLAKIKPNVFATEGVIEALCSLLRRDASINFKAKGNSLVSVLACNLLMAAYEEDENWPEIFVKVYIEDSLGERIWVDSPHCKTFVDNIQTAFTTKMPPKSMLLQTDAGRSGGDLSAGSSPHPSTPDEDDSQTELLIAEEKLSPEDEGQVMPRYDELAESVEDYVLDVLRDQLNRRQPMDNVSRNLLRLLTATCGYKEVRLMAVQRLEMWLQNPKLTRPAQDLLMSLCMNCSTHGAEDMEVVSNLIKIRLKPKVLLNHYMLCVRELLNAHRDNLGTMVKLVIFNELSNARNPNNMQVLHTVLQHSPEQAPKFLAMVFQDLLTNKDDYLRASRALLREIIKQTKHEINFQSFCLGLMQERKETTYVDMEFKERFVIQVTDLLTVSMMLGITAQVKESGIAWDKGEKKNLEVLRAFQNQIAAIQRDAVWWLHTVVPTISKVGAKDYVHCLHKVLFTEQPETYYKWDNWPPETDRNFFLRLCSEVPLLEDTLMRILVIGLSRDLPLGPADAMELADHLVKRAAGVQSDDLEVLRVERIQLIDAVLNLCTYHHPENIQLPPGYQPPNLAISTLYWKAWLLLLVVAAFNPQKIGLAAWDGYPTLKMLMEMVMTNNYSFPPCTVADEETKNEMISRELQISQREKQEILAFESHLAAASTKQTITENNSLLLSQLTSLDPQGPPRRPPPHIQEQVKSLNQSLRLGHLLCRSRSPDFLLNIIQRQASSQSMPWLADLVQSSEGSLDVLPVQCLCEFLLHDAADDSSSAEDDEEGESKEQKAKKRHRLQKQRQLLGRLQDLLLGPKADEQTTCEVLDYFLRRLSSSQVASRVLAMKGLSLVLTEGGLRDGEEREQPMDEDSRDAELLPAYQWLLQDLPRLPLFESVRSMTSAALQQAVHMETDPQTISAYLIYLSEHAPIEEQALHNDLALDVARLIVERSTIMNNLFSRHLYRPESDAVLSALISIFSKYIRRMRKTKEGEDLYSWSESQDQVFLRWTTGETATMHILVVHAMVILLTLGPPKGDSDFYTLLDVWFPEKKPLPTAFLVDTSEEALLLPDWLKLRMIRSEVSRLVDAALQDLEPQQLLLFVQSFGIPVSSMSKLLQYLDQAVSHDPQTLEQNIMDKNYMAHLVEVQHERGATGGHTFHTLLSASLPPRRDSADANQAKVTGETPHGAVRIRPASQIPVIGPDDDLAGMFLQLFPLNVDMRWQSSNPQQVSLALQQALAQELARVRQGHPQVTGVALRLLQAVAALLTSAHSGALVLAMHRGHAVSCPLLRQLHLYQRCVPQDVAFSSLFFKAVMQMLTWLENPAVEPGPLHALLKSFAAQFSIKHRLSDVRTGFVHLAEALAYHRDSEVAVRAVIATLKAGEKCNAEPELMGKVIKGLIEVKSPYLEELLALLMTVGMETGAANPGTGPVAMVNSLLLQECEEASVKAESDSSTSDVTKTVNSSGLLIDWLELLDPEVISVLPDLQQRLLFSWNKSKGNAGAQIPSYRPYLLALLTHQSNWTTLHHCISFLLSKHKELRLEPSSSLDFLWACIHIPRIWQGRDQKTPQKRTENFVLRLKAPELISLVDLILSESEMNSHTPPQSRRNLDEASCSLIQSRLPLLHSCCHGDLESVKKVSEYLINCIKKWGESVMSKRCQNLLLQIYLQVPEVIQHVTLPGVTLSSEGAAAGSTCKLDVLVHRLITLLADTGESKSAESRMSDANMACRKLAVAHPVLLLRHLPMIAALLHGRIHLKFQEFRQQNHLTFFTHVLSILELLQPLVFHSEHQRALQDSLLTFTQVLQNFRKSSRQLSTFISKFVQFIQKYITHDASAAVPFLQKHSDILQGLSSENPDLVQLKSLLAGLTLPVRSGVSETVSEDREAEESSAGSLPLVSISASAPLTASDMAKYLKNIARGEAIEDILEVLSEVDEKSKRSPEIIPYFTSDLQRLMSSSEELCRNMAFSLTLRCIQNNPSMAADFLPTFMYCLGSGNFDVVQTALRNLPEYVLLCQEHADILLHKAFLVGIYGQIDTSSIIAESMKILRMEATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZY9</accession>
    <name>W5LZY9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SCY1-like, kinase-like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZY9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001696"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001701.1">
        <property type="protein sequence ID" value="ENSLOCP00000001696.1"/>
        <property type="gene ID" value="ENSLOCG00000001482.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691220"/>
    <dbReference type="KEGG" id="loc:102691220"/>
    <dbReference type="CTD" id="57147"/>
    <dbReference type="eggNOG" id="KOG1243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151043"/>
    <dbReference type="HOGENOM" id="CLU_015864_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZY9"/>
    <dbReference type="OMA" id="HDPELDW"/>
    <dbReference type="OrthoDB" id="9942861at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001482">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF15">
        <property type="entry name" value="PROTEIN-ASSOCIATING WITH THE CARBOXYL-TERMINAL DOMAIN OF EZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="338"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="578"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="615"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="761" mass="84526" checksum="492549F978984EBD" modified="2014-04-16" version="1">MGSDSSTLKSCTLEECQLSLPSGLTMYSALLEDGKPASVFVYRPDSEDKVNKAAKHLKTLRHPCLLRFLSCTVQAGGIHLVTEHVQPLEAMLESLSPEEICSGLYDILQALVFLHDRGKSSHNNVCSSSVFVSDDGHWKLGGMETVCKFSEASPEFLSSIGSVREQRVVPPEEKVEGFKMLLDKHGHARDAFSFGLMVENLIPLLNDYASKELLDSFRNTLQRSLLNPDPSARPALSSLLKHDFFRNDFLEVVNFLKSLTLKTEEEKNEFFKFLLDRVQNLPEELIASRLIPKLLNSLVFAEPTAVKSFLPHLLMPKKDSSENSQECLLSVSLYRKYVIPQLLKLFKVNEEHVRMVLLSHIDVYAELFSHEELKNQILPQVLLGMRDTNDCLVAMTLQSLAILVPLLGAHVVVGGERSKVFKRTTPNFTKSTEVTPEGSPVHSVNSFKTQVSQPSKVLKLFSKSSEAAEFVLENMGSLGSIAQSQQVPKKTVNRDLKKLPLNGFNDRRPPISAGEENLHGLGSIARDGEEWPDWSDAEEMEKNKTVELCVQPVDTQKTVNITRDINMEEEPWEDLETCNHGSEMTSHQASHGSAGDSTASPLGDPPKNPFPGPTKKSNALKLSSASKPKDEKRQTDWDNDVVHLSAKPSQMQKVISGGLGEEFTIEIRKKPEKDPEMDFFADMTPEIKLSSATLMFTSERTDAVFGSSLAVPSGHTELLDQESSTDRLTIIAKFAAADLADTEASGWGDSDDLNWEDESNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0H1</accession>
    <name>W5M0H1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hypoxia-inducible factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01033986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0H1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001878"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001883.1">
        <property type="protein sequence ID" value="ENSLOCP00000001878.1"/>
        <property type="gene ID" value="ENSLOCG00000001634.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156774"/>
    <dbReference type="HOGENOM" id="CLU_1387072_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0H1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001634">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000015">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF7">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="21835" checksum="A91FA3552B7FD8E1" modified="2014-04-16" version="1">PGSFPTRVVELTGYEESELLGRSVYQYYHALDSRHILKTHHNLFSKGQSSTGQYRLLVKRGGYVWVETDATVVYNSRSGQPQSVICVNYILSEPLESEVIFSLEQTEDLSPCPVAGEPLPLCAPLSGGWGQLARAPAETTTALYWERGQPGEPCATRSPGAQWGWMYITLWLLHQFCTSASGGQQGPESIRSRVNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M176</accession>
    <name>W5M176_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD repeat domain 88</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M176"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002133"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002138.1">
        <property type="protein sequence ID" value="ENSLOCP00000002133.1"/>
        <property type="gene ID" value="ENSLOCG00000001845.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107079984"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162291"/>
    <dbReference type="HOGENOM" id="CLU_1481504_0_0_1"/>
    <dbReference type="InParanoid" id="W5M176"/>
    <dbReference type="OMA" id="MENAIYI"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001845">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45048">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45048:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="86"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="19654" checksum="087D22EBE0170C96" modified="2014-04-16" version="1">MATARSSVDPLSVEEATEDTNEGDKGTPLWEHQELSQIPIKVLRGHTDAVSSCSFCFEDSRIVTSSHDRTAKLWDAESGVPLLVFDRGHSAPITGCALTPDHNRLITASWDKTMKAWDMKTGEVLWSAAHDGLLTSCSVSPDGNYVASSTDMENAIYITCVDSGEKIFKIKGGTFAFLGGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2L3</accession>
    <name>W5M2L3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01003315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2L3"/>
    <dbReference type="FunCoup" id="W5M2L3">
        <property type="interactions" value="967"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002621"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002627.1">
        <property type="protein sequence ID" value="ENSLOCP00000002621.1"/>
        <property type="gene ID" value="ENSLOCG00000002250.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153680"/>
    <dbReference type="HOGENOM" id="CLU_034296_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2L3"/>
    <dbReference type="OMA" id="IRMEYES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002250">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16550">
        <property type="entry name" value="RING-HC_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051657">
        <property type="entry name" value="RNF168/RNF169_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="376"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="462" mass="50845" checksum="000AAEB6ED284AD6" modified="2014-04-16" version="1">RKMAALRSAEYASTGPGPRPRPGAGAPPRQLTLEEARCPVCLEILLQPVTMPCGHSVCLPCFRRTVELSSLCCPLCRLRVSSWARRQAREKRLVDAELWERVRQSYPERCRRRMESSVGRMFDGELLLTGIIFCKPRKIQQEYKKQMKNKVTGSVEHEVKKKVQSHWKKDECGTLKCFQEPRAVVLSDSENEEPMGGRTKHMSAFKKTRNSPACSKRVQTLVRQRSRSCTDPEEGQLKSSCLSHVAAMVKTNVTYSTNAGILLSSENSCSFSAPILTPDKRQSGRSGAGPPAPLAGPPSKPERSISPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVVEAAIVKSTPRNISRSLQKHTSYEASPTILQKWKQIEMDRQSTKAASKGTITSSPAEEFPLRQSPEGVACQPASAKKKTPSGHAALAKESLNRSLPAAVPQADGSGHEKGKASAGNKRRLVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M304</accession>
    <name>W5M304_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GPCR family 3 nine cysteines domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M304"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002762"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002768.1">
        <property type="protein sequence ID" value="ENSLOCP00000002762.1"/>
        <property type="gene ID" value="ENSLOCG00000002357.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="HOGENOM" id="CLU_100070_0_0_1"/>
    <dbReference type="InParanoid" id="W5M304"/>
    <dbReference type="OMA" id="INCPQDY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002357">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF519">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="12">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="GPCR family 3 nine cysteines" evidence="13">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="18808" checksum="30149CE286A6D401" modified="2014-04-16" version="1">VYKAVYAIVHALHDLLFCAKEKGTTVLCGDVSRIEPWQVTKHLKRVNFVNRFGEAVYFDENGDPPAAYDIINWQLNKGVVSHVTVGHFDTSPDGGSQLVIDEDSIVWSTGREVPAGVCSESCPPGTRRAARKGQPICCFDCIPCADGTIANTTGAAECINCPQDYWSNDGKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3H5</accession>
    <name>W5M3H5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Breast carcinoma amplified sequence 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3H5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002939.1">
        <property type="protein sequence ID" value="ENSLOCP00000002933.1"/>
        <property type="gene ID" value="ENSLOCG00000002499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694320"/>
    <dbReference type="KEGG" id="loc:102694320"/>
    <dbReference type="CTD" id="8537"/>
    <dbReference type="eggNOG" id="ENOG502S50P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00650000094602"/>
    <dbReference type="HOGENOM" id="CLU_031051_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3H5"/>
    <dbReference type="OMA" id="SASVPHH"/>
    <dbReference type="OrthoDB" id="8962384at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
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</entry>
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <sequence length="507" mass="55461" checksum="627079D4EEC57411" modified="2014-04-16" version="1">KWLEAHYDPLANLYTFSSCIALGGTATPNSSQLVVGDLGTGVYNMKLKVYRGTSLVSENTLLDLPTGLVAFLMDQHEPRTPAVAVASGPFIYIYKNLRPYFKFTLPPLEVNALEQEVWSQAREDKIDPMSLKEMLEGIREKADVPLSVRSLKFLRLLRDLFVPLKVVSRDIALSLSGCVMSLPLPLRGTGGQNRSQSLVIIIIIITTTTFLLISVYFSCLSPSWKQSGARAQGDMRFRGAESVLLSSVCVCYRESTKPKYCIELSSHPVGLVRVGKNVVVGCTQETLHGFTQKGKKLWTAYLPAPITTMSLMDHHARGFQAVLVGLTNCQVHMYCDKNLVGVIKTPDMVTSICFGRYGREDGALIMTTKGTHLAVQTEQECASPRCRDEELQSPAGVTVGMRSSRVLRVLLDEELQSPAGVTVTGLAAPVSSSPPERGSASLARARACRLCSPVPCRPPPQVQGLGPSFKLTLNIQNTAACRPVMNLAVSFLYDESLYSMKSAFFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
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                <person name="Alfoldi J."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="58"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="2954"/>
            <end position="3013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2869"/>
            <end position="2902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2924"/>
            <end position="2957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2932"/>
            <end position="2955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3013" mass="347595" checksum="602BC5649EA40D51" modified="2014-04-16" version="1">SYNLPVDMLGFVLAKKNQIQVSDIDYRRYLHQWTCLPDQNDVIQAKKAYELQSDAVYKADMEWLKGIGWVPIGSLDVVKAKKAGEILSDRLYRQHPDTIKFTSVTDSLPIVLAKSNALNMNDRLYKEAWNKDKTTIHVMPDTPEIVLAKANSVTVSKKIYKQDYEDSLKKGYDMRADAIPIKAAKASRDIASDYKYKEGFRKQTGHHIGARCVKDDPLLMLALNAAKIASDAEYKKDFQKSKTKYNLPVDMLGFVLSKKCQIQVSDIDYRRYLHQWTCLPDQNDVIQAKKAYELQSDWVYKSDLEWVRGCGWMPNGSLEVNKVKRAQEILNDRLYRQHPSTVKFTSVVDLPAIVLAKNNAIQLSDLKYKEAWEKEKTISHVMPDTPAIVLSRANAINVSQKLYTQAWDEQKAKVYNIKEDAISVLKAKASRDIASDYKYKEGFRKQTGHHIGARSVKDDPLLMLAINAAKIASDAEYKKDFEKSKTKYNLPVDMLGFVLSKKCQIQVSDIDYRRYLHQWTCLPDQNDVIQAKKAYELQSDAVYKADLEWLKGIGWMPNDSLDVKKVKHAQHILSDLEYRTKLDTLKFTPVVDRVDYVTAKQAAEILNDAKYREAWETAKTKYTMLDTPLLATAREAAKNINQKLYKESWDKEKATSYFIPQDSVEIKHVKHSQGIQSNYKYKEGFRKQTGHHIGARSVKDDPLIMLAINAAKIASDAEYKKDFEKSKTSYNLPVDMLGFVLSKKCQIQVSDIDYRRYLHQWTCLPDQNDVIQAKKAYELQSDFVYKSDMEWIRGCGWVALDSVDHQKVKKAQEILNDRAYKKGAKEGFAKFTHVVDRPEIVLAKINAANLSDLKYKESFNLEKGHYIGSEDTPQLAHSREVAKIISEKVYKSNWDGTKATSYQLPPDHLPLVGAKHTKQIVSDIKYKEAHEKAKGHYLAGKDIRDFPNVVHSSAIEKMKSLMTYRKAYDESKTKIHIPFDMLSHVMAKKCQHILSEVEYRQRLHQWTCLPDQHDVIHARKANEILSDVFYKEDLTWLKGVGCYAWDTPEIVRAKKSYDLQSELLYRDAGKKEFKNFSVVTDTPVYVTSVTGYIQASDLHYREAYHKSKDKYTTVLDTVDYARTQGLKSLYSNKTYTDVWDKIKATSYKLPSDSFSLAIAKNQKNITSALKYREDYEKFKALYTLPKSVEDDPATARCLKVGKLPIDRLYKENYEKTKAKVHILPDMLEIVAAKNTQKMISEIDYRKRLHEWICLPDLQIHMHARKVNEQLSDITYKDDLNWMKGIGCNVWDTPEILRTKHSYELQSDLKYKAKAEEQKKNFTVVTDTPTYVQAVISANQLSDLVYRHDYVKNVKGTNTAPAVTVDLERAREANKIQSDNLYKEANYHLMPTGYTLPPDTPANLQAKKCSIINSNQKYKEAFELVKAKSYTVPHDSVNFVNLRKVNESINERLYRQLYERTKDKIHSVYDTPEIKQVKATQEAISDLCYKEKYFNMRGNLISMPTTPELMHCRYVNDITSDVRYKEDLQWLRGMGCFLFDTPEMVRVRNVTKLRSNYDTEAKKLHGKFSVVLDTPEYRTVTELKSHLSSLVYRAAGNEQKTKYTPILDSVDLNRAKLGQQIQSQYLYTELATKERPHFTPEAQTPALTHAREMKVITSEKKYKEQYEKMKMKYTPIADTPTLLIARKAYLNASDLRYKESFEQAKGHYRTVKDALDIIYHRKVTDDISEVKYREKYISSLGLWRSIPDRPEHFHHRMVSEAVSDVKYKEDLSWLRGIGCYVWDTPDLVLAEKNKELYSVNKYRASFEKNRANFKYTADTPVYRTAKYAAQLLDEKLYRAEYEKTKDKYSIVVDDPRNLLAKEVNKMSQWRYKQSYEKEKDKYTTVLDTPENRKHKYTKKMISDIVYKMEYNKTKAKAYTLGHDTPHLQHMKKVKERLSQLKYKEEYEKNKAHITIAPDAHEIRAAKEAYKNITNLDYKKKYEATKAKWIWTVDRPDFIHAVKTSQQQSDVDYKYDREMLKGCKLSVTEDKYLTLAKKGSEMASAVKYKEKYEQAKGTYQVVSDTPQILHAKSMASLVSENKYKEASKKELPQGSFTTLPETRDTVHVKEVSKILSKKQYQEKFEKEKGKSNYALMLAPPDVKHAMDVAKSQSNIAYKQEAKAKLHYTSVADRPDIRKATQAAKLISDIGYRDKARKEASRGGSLVNRPDIELATEVSKLTSQVKYKEKFDKELKGKRPQFDLKESKIYQTLKDANNLASEVKYKGDLKKIHKPVTDMSESLSMQHNLSTSKLSSDYQYKKKFEASKGHYHMVPDTPEQIHHKEASELQSQVKYKEKYERERGKPMLDFETPTYVTAKEAQHMQSEKLYKKDFEESIKGRNLSGLEVTPALIHVRHATKIASEKEYRRDLEEGVKGKGLTVLEETPDLLRAKNATHILNEREYKKSLEQEIKGKGMLALATDTPDFMRAKNATEILSQSKYKHTAEQDRASYTTVIDTPEIIHAQQVKNIVSQQKKYKEEAEKTMSHYVPVIDTPEMQRVRENQRNFSTQQYQIDLKNSKGKVSVVKDTPEMLRVKENQKNFSSILYKEDLGHGTAMKETPEMERVKRNQQAISTIKYKEAVGTGTAIPDLPEVKRVKDTQKNISSVVMFWKPTLTSRFPIELFTVLWSIKLNIFNLDNHKFNMLWSVLRGPETVVGCAKGRVSANCLTSSLVLYKEHLSTGTPVTYTPEMERVKRNQEHISSVLYSDSFRKQVQGKAAFVLDTPEMRRVKETQRIISGVRYHQEFEKSKGSFTPVVTDPITERVKKNMQDFSDINYRGIQRRVVEMERRRVAEHDQETITDLRVWRTNPGSVFDYDPAEDNIQSRSLHMMSVQAQRRSREHSRSASALSLSAGDEKSETSEAAEQHLSLYSNGGFFNASSSGYQHAKTTELPQQRSSSVATQQTTVSSVPSHPSTTGRTVRAMYDYTGADSDEVSFKDGDVIVNVQSIDEGWMYGTVQRTGKTGMLPANYVDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4A0</accession>
    <name>W5M4A0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the LTBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4A0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003208"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003215.1">
        <property type="protein sequence ID" value="ENSLOCP00000003208.1"/>
        <property type="gene ID" value="ENSLOCG00000002729.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160285"/>
    <dbReference type="HOGENOM" id="CLU_1032631_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4A0"/>
    <dbReference type="OMA" id="KWHEGEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002729">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000302">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF200">
        <property type="entry name" value="EGF-LIKE AND EMI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TB" evidence="9">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="29257" checksum="C58268749FC2B65E" modified="2014-04-16" version="1">MQGVCRNGECLNTQGSFRCACKPGFVQERARCVDVPGETGPCYRMVTVEGQCEHALPTELTQEMCCCTVGKAWGRGCETCPQVGTASFTKICPAGKGYSLQSYRQIVSIQPQNFHPGAAEQGPKQPGKPALTPKPMEPDRKPTPPRKNDSEDPEATTVVQPASSSTHRPRLQEVMSRPTLSPVVNVPAEPHPKMPMEIGQTKFTQTDECRLNRNICGHGECINSANGFACLCNTGYKAHAQRKYCVDDNECEAEPCGSGRGQCINTGGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4E0</accession>
    <name>W5M4E0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute carrier family 12 member 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4E0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003248"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003255.1">
        <property type="protein sequence ID" value="ENSLOCP00000003248.1"/>
        <property type="gene ID" value="ENSLOCG00000002752.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2083">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160130"/>
    <dbReference type="HOGENOM" id="CLU_017440_1_0_1"/>
    <dbReference type="InParanoid" id="W5M4E0"/>
    <dbReference type="OMA" id="IMFIIQW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
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        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:potassium:chloride symporter activity"/>
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    </dbReference>
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        <property type="term" value="P:cell volume homeostasis"/>
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        <property type="entry name" value="solute carrier family 12 member 8"/>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
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    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="212"/>
            <end position="232"/>
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            <end position="309"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="390"/>
            <end position="409"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="415"/>
            <end position="438"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="621"/>
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        <location>
            <begin position="653"/>
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            <begin position="72"/>
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            <begin position="497"/>
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        <location>
            <begin position="502"/>
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        </location>
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            <begin position="514"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="740" mass="81425" checksum="D0A29625C35A6BE3" modified="2014-04-16" version="1">MEASPFSWFVSSRCPAEDRSVLLESEKSRGVAGRVQELFHEDAQGSPSHTQPWWRVKLFVWEPVLFGTWDGVFTTCMINIFGVVLFLRTGWLVGNTGVFLGMLLVSLVVVVALVTVTSGIGVCERCSVGSGGVYSMISTVLGGRVGGTVGLLYVFGQCVAGAMYITGFAESIADVLSFNSVWAVRGISFAVLLGLLGINLAGVKWIIRLQLLLLAVLAVSTLDFVVGTFSHLDPEHGFVGYSEELLQNNTLPAYSPGESFFTVFGVFFPAATGVMAGFNMSPDLQKPDYNIPVGSLAAIGISLFLFLFFSMKMNLSNHRSNYRTVRLQIKERSLKPILPFKHSVSLPCQRQCLTSACYGSCRIKAAQQRQVFDHDPVFVLQQRGPNKTPVAAICLTSLITMAFIFIGQVNILAPIVTINFMLTYSFIDYSYFSVSMSYKLQMKLRKPLPPEGSAHNSSKPLILTKHSRYGTDDIVKTQRNGTLLEFTKDMDQIFQSPGPATEKAKKEKESAVSHRGKFRKSRKPAKQMLMDSFQLEFNSSKTADQENGEASGTEVLHSGGWSEAAITDARITPYVVFRTLMDFMLMTSNLSSTGLTEKQKLQGAEIQQLPTSLHTRLCNHWVSLIGAICSVAIMFVIQWIYAIANIAMATVLYFYIGQANPGLPTGVSANFSFFKWLQSSLKTMGRRGPAPQDQIVVTPSFAAVGMETQQLTEENADFASRDRYHHSSIITGGELTSQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5R3</accession>
    <name>W5M5R3_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Vimentin type intermediate filament associated coiled-coil protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011184">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000003155.1"/>
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        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003155">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:type III intermediate filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="20581" checksum="7A608EBB53781164" modified="2014-04-16" version="1">MSLPSPVQIKEANAHLAAVHRRVSELERRLEAAENTVREQAESLIRKDEQLRAATQEIREAKEREISFLHEKLCKSEETIQRFQNLVKEKDALIGQLRHRCRLLDNICKSRPLLDNMLAHMAEAERLGPVAGLGEAARSPPLADGEINCSPERMANHRDFSLSEDDMDEQDLEETMFGTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M665</accession>
    <name>W5M665_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Core component of the TRAPP complexes which has a function of guanine nucleotide exchange factor activity for Rab1 GTPase. Plays a role in vesicular transport from endoplasmic reticulum to Golgi and autophagy. May play a role in dendrite postsynaptic membrane trafficking.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with SDC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus</location>
            <location evidence="15">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642231.1">
        <property type="nucleotide sequence ID" value="XM_006642168.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M665"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003873"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003881.1">
        <property type="protein sequence ID" value="ENSLOCP00000003873.1"/>
        <property type="gene ID" value="ENSLOCG00000003274.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693185"/>
    <dbReference type="KEGG" id="loc:102693185"/>
    <dbReference type="CTD" id="51399"/>
    <dbReference type="eggNOG" id="KOG3369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153761"/>
    <dbReference type="HOGENOM" id="CLU_053380_1_0_1"/>
    <dbReference type="InParanoid" id="W5M665"/>
    <dbReference type="OMA" id="GQRDGIN"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003274">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trafficking protein particle complex 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trafficking protein particle complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="219" mass="24427" checksum="1B5A02D5614DBB01" modified="2014-04-16" version="1">MAIFSVYVVNKAGGLIYQYDNYVPRAEAEKTFSYPLDLVLKTHDERVIVSFGQRDGIRVGHAVLSVNGVDVNGRYTADGKEIIEYLKDPSSYPVSIRFGKPRLSSNEKLMLASMFHSLFAIGSQLSPEVGSSGIEMLETDTFKLHCFQTLTGIKFIVLADPRQSGIDALLRKIYEIYSDYALKNPFYSLEMPIRCELFDQNLKSALEVAEKTGTFGPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6D7</accession>
    <name>W5M6D7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mannosidase endo-alpha like</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6D7"/>
    <dbReference type="FunCoup" id="W5M6D7">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003945"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003953.1">
        <property type="protein sequence ID" value="ENSLOCP00000003945.1"/>
        <property type="gene ID" value="ENSLOCG00000003339.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPJV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016054"/>
    <dbReference type="HOGENOM" id="CLU_042710_1_1_1"/>
    <dbReference type="InParanoid" id="W5M6D7"/>
    <dbReference type="OMA" id="WRKNDER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003339">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11574">
        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000019">
        <property type="entry name" value="glycoprotein endo-alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF2">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="456" mass="51666" checksum="4DC5535AA9CDA922" modified="2014-04-16" version="1">MARLRKKACVALFLFTLFIFGTMMGLRTLKPSDGFSDLAPGLELMPVAGDRADRRSISNEVVASPAQPRAVGNAKTALWNSSPDKGIFYDVHIFYYVWYGNPKVDGKYLHWDHVLVPHWDPKVAASYPKGRHTPPEDIGSSFYPELGPYSSRDPVLLESHMEQLKASGAGVLVLSWYPPGLADDNGEAAEDLVPAVMDAAHRHRIKVAFHIQPYKGRTDRTVHDNIKYIIDNKRYGNHGALYRFKTSTGKILPLFYIYDSYLTQPGSWADLLTVSGSHSLRNTPYDGVFIALIVEERHKHDILSSGFDGMYTYFASNGFSFGSSHQNWKAIKTFCDANNLMFIPSVGPGYIDTSIRPWNNHNTRNRVNGKYYETSLQAALMVRPEIVTITSFNEWHEGTQIEKAVPKKTATRIYLDYQPHKPDLYLELTRKWAEQFNREKEQWLILIPTAAEPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M762</accession>
    <name>W5M762_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytospin-A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SPECC1-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Sperm antigen with calponin homology and coiled-coil domains 1-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">May interact with both microtubules and actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytospin-A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M762"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004220"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004228.1">
        <property type="protein sequence ID" value="ENSLOCP00000004220.1"/>
        <property type="gene ID" value="ENSLOCG00000003564.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153592"/>
    <dbReference type="HOGENOM" id="CLU_009328_2_0_1"/>
    <dbReference type="InParanoid" id="W5M762"/>
    <dbReference type="OMA" id="NGPCHAV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003564">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000020">
        <property type="entry name" value="Cytospin-A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF3">
        <property type="entry name" value="CYTOSPIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0303">Gap junction</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="877"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="182"/>
            <end position="272"/>
        </location>
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        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="276"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="483"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="182"/>
            <end position="200"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00033100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004220.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="994" mass="111272" checksum="01E96435C6E7602F" modified="2014-04-16" version="1">MGNQAGRVEELEQGVPSAKKSGIPAPREIPTTISRDRLSLRDQLRTASTKKVVPSASTSSLSALAKHSRPPVKAKSDGETNDKAILECQVKELLAEAKAKEFEISKLRTELKRCRDRGMLDVVTPEGTDLDLESLVAQPAEIQSLVKTMREKNRTFQRELAALREENQTLKEKCFIMEHSSLSSSNNSNASPPTSLASPAKNPVNGIVLDTSRTPINGSTLKNSSSSSSDITKGSPSPDSSEFEKIPSRSDSIGNSTGKSKGSTGPNSPQARDLSVECLTEQIQKMEESHHSTAEELQATLQELADQHQVVQELTAENERLAEEKGLLQTSLQQQRERVENLVQKNESLLARLQEQSKSEESEARAARISELEQRYAELVESSRFEREKLVDIQQQLTSSLRSLEKEHQEAQGLIRTLKEEKDLLQNRIEKEQEVGSKITRTLEECNSSMEALKVENGRLKVQMDIERQKVAELKAMQSASDNTELQHLLKAAHSEKERLELSCTELKQELLKANSEIDRVKGMLSKAEAECQRLQELCASQEKEVSSSAQRLEERAAEKDTQIKDLKETIFELEDQVEQHRAVKLHNNQTILDLENQVKKLEEQKSDLEKQIKALNKQMKDDAEEWRRFQADLQTAVVVANDIKVEAQQELRGLRRRLQEEQERSAKLAGEVEQLQGSRLKNEEAYSSDTDTSPHWCGISVSQIPTASSESGTTVKSLIKSFDSALQGKMPFILPASLFPSDLAVCPVRIFPKNSYAVSIYRCITQKLARTLKKNDKHLLILQKKCLLPGGTNTNPQETGSETALKLFFFPFPPIKIVWVFIWFSDKLTGSNDEMKPTSLMRKSPSLESVIKTPAALSGRTSSLSYPKANSKLSSQEMCFTAILKLENGGCMCSDLCCISLSSWQNIDVTNFSSSWSDGLAFCALLHTYLPAHIPYQELLSQDKSRNLTLAFQAAESVGIKPSLDINEMMHTDRPDWQSVMQYVAQIYKYFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M767</accession>
    <name>W5M767_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Docking protein 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOK family. Type B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004321">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004323">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209286.1">
        <property type="nucleotide sequence ID" value="XM_015353800.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M767"/>
    <dbReference type="FunCoup" id="W5M767">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004225"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004233.1">
        <property type="protein sequence ID" value="ENSLOCP00000004225.1"/>
        <property type="gene ID" value="ENSLOCG00000003566.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686350"/>
    <dbReference type="KEGG" id="loc:102686350"/>
    <dbReference type="CTD" id="220164"/>
    <dbReference type="eggNOG" id="KOG4047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158760"/>
    <dbReference type="HOGENOM" id="CLU_057256_1_0_1"/>
    <dbReference type="InParanoid" id="W5M767"/>
    <dbReference type="OMA" id="FQIFRRC"/>
    <dbReference type="OrthoDB" id="6279276at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="entry name" value="PH_DOK4_DOK5_DOK6"/>
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        <property type="entry name" value="PTB_DOK4_DOK5_DOK6"/>
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        <property type="entry name" value="Docking protein 4"/>
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        <property type="entry name" value="Docking protein 5"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF43">
        <property type="entry name" value="DOCKING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="3">
        <location>
            <begin position="132"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="338" mass="38768" checksum="0C7C466DE8F2A356" modified="2014-04-16" version="1">MASNFNDIVKQGYVKFRSRKLGIFRRCWLVLKKASSKGPRRLEKFPDEKAAYFRNFHKITELHNIKNITRLPRETKKHAVTIIFYDETSKTFACESELEAEEWCKLLCVECLGSRLNDISLGEPDLLAAGVQREQNERFNVYLMPTPNLDIYGECTMQITHENIYLWDIHNARVKLVMWPLSSLRRYGRDSTWFTFESGRMCDTGEGLFTFQTREGEMIYQRVHSATLSIAQQHERLMIEMEKNAQIQSGSNEGALSKSVSLPRSAYWHHITRQDSVGEIFSLQGASLGMTLVPRAQTFPSHLLEQGADEELARIRHQEQALSPSSGYGSSYSSGPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M822</accession>
    <name>W5M822_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Latexin</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the protease inhibitor I47 (latexin) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M822"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004530"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004538.1">
        <property type="protein sequence ID" value="ENSLOCP00000004530.1"/>
        <property type="gene ID" value="ENSLOCG00000003797.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYUY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063813"/>
    <dbReference type="HOGENOM" id="CLU_083048_0_0_1"/>
    <dbReference type="InParanoid" id="W5M822"/>
    <dbReference type="OMA" id="MWQNSTE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003797">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000007">
        <property type="entry name" value="latexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049897">
        <property type="entry name" value="CYSTATIN_LXN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009684">
        <property type="entry name" value="Latexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28591">
        <property type="entry name" value="LATEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28591:SF1">
        <property type="entry name" value="LATEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06907">
        <property type="entry name" value="LXN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52033">
        <property type="entry name" value="CYSTATIN_LXN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865686" evidence="6">
        <location>
            <begin position="18"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin LXN-type" evidence="7">
        <location>
            <begin position="66"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin LXN-type" evidence="7">
        <location>
            <begin position="185"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="33790" checksum="6B68779F7C10937E" modified="2014-04-16" version="1">MRWALCVALGLLPLVCGHPTSGTGQEELQDADPLRLQSGSTSGRAHEVPAETNPLPETTEVNPAMPVEELNPSHYQASRAAKVAEHYLNYHYGSPFRRFIIRQVKRATLEEIPTGAARYHIEFSLEEMVSNTTIGNCAAEIVFERTEEQKTPQVQCTCDDLLKINTSEAENSFFHQIKKSSALVSGQYIPDSYGFTAPEMTPLWHLGGVAASFVMLKESNESTLYNMAQILSLQQQESEGESLEFDYEVLLHEFISQEMIQWHMKVNWSPTEGVKVTQSNLQPKHHDHSPTTENPVINSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8H7</accession>
    <name>W5M8H7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Trophoblast glycoprotein 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8H7"/>
    <dbReference type="FunCoup" id="W5M8H7">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004685"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004693.1">
        <property type="protein sequence ID" value="ENSLOCP00000004685.1"/>
        <property type="gene ID" value="ENSLOCG00000003925.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154868"/>
    <dbReference type="HOGENOM" id="CLU_064866_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8H7"/>
    <dbReference type="OMA" id="SRALYNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003925">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052286">
        <property type="entry name" value="Wnt_signaling_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24364">
        <property type="entry name" value="LP06937P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24364:SF22">
        <property type="entry name" value="TROPHOBLAST GLYCOPROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865853" evidence="5">
        <location>
            <begin position="24"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="6">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="7">
        <location>
            <begin position="242"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="40661" checksum="14CAEA8B785A6F62" modified="2014-04-16" version="1">KMFSAVVRVSLCALLLSPGLCGACPFPCECSEAARTVKCVLKDLKDIPAGIPGYTRNLFITGNQIRRIGPKSFQGLENVTTLSLSNNRINTVESRAFAGLRSLRSLDLSTNQLSGIHPEAFLLENSSLQELDLSQALYNHSAVTPLASSLRLGGGRGLGRLLRLDLSDNGLIFLPPGMFANLSGLQQLDLSNNSLVAIMDSTFLGLDLRKLDLSLNAFKTFKEDSLRELGGQRQAQILLRDNPFVCNCDVEDFARWLNSTSGVPDADRLVCVDPRELHNVSLLSLGELALECHASKGADLALQTSYVFLGIVLGIVGVIFLFVLYLNRKGIKKWIYETRDACRELMEGYHYRYEIESDPRLAQISTTSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9Q1</accession>
    <name>W5M9Q1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase Sgk1</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Serum/glucocorticoid-regulated kinase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Protein kinase that may play an important role in cellular stress response. May be involved in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01009574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9Q1"/>
    <dbReference type="FunCoup" id="W5M9Q1">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005110"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005118.1">
        <property type="protein sequence ID" value="ENSLOCP00000005110.1"/>
        <property type="gene ID" value="ENSLOCG00000004269.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164496"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_48_1"/>
    <dbReference type="InParanoid" id="W5M9Q1"/>
    <dbReference type="OMA" id="GLMMCEM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004269">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05575">
        <property type="entry name" value="STKc_SGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000065">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000030">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="10"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="154"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="412"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="56065" checksum="D7CA7C6014339695" modified="2014-04-16" version="1">KTQKNKHPSIFLLCYSITLNMTKKQQKQYNKYKIFVTNNRDSWFVIRQYKDFVKLFKMIKKQYPDAQLNQPQRKLFGRKYTAAFIKERKMGLNDFIQKLVSNPHICQHSDVRVFLKIDENQNEETELESGSTPVCLNSPRSSLAEETQVKPSDFDYLKIIGKGSFGKVLLARHKESAKYYAVKVLQKKIILKKKEQKHIMAERSVLLKNLKHPFLVGLHYSFQTTDKLYFVLDYVNGGELFYHLQRERVFLEPRARFYAAEMASALGYLHSLHIVYRDLKPENILLDSQGHIVLTDFGLCKEGLDPNGTTSTFCGTPEYLAPEVLQKQAYDRTVDWWCLGSVLYEMLYGLPPFYSRNTAEMYNNILHKSLVLKPNVSNAGRELLEGLLQKDRRRRLGARDDFLELKSHAFFSPINWDDLVAKKITPPFIPAVSGPTDLRHFDPEFTHLPVPGSLGNTDSILVTSSVREAAGAFPGFSYVPPADDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA04</accession>
    <name>W5MA04_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01004363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA04"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005213"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005221.1">
        <property type="protein sequence ID" value="ENSLOCP00000005213.1"/>
        <property type="gene ID" value="ENSLOCG00000004365.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162286"/>
    <dbReference type="HOGENOM" id="CLU_000680_32_6_1"/>
    <dbReference type="InParanoid" id="W5MA04"/>
    <dbReference type="OMA" id="TIHMAFH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF28">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="405" mass="47361" checksum="53CFD4FD08ECEFBF" modified="2014-04-16" version="1">PPETWQTSITVPIWKDKGDVNDCSNYKLLCHTMKIFERVLDAQLRTIIDISPNQCSFVKNCRTIDAARLLMERHHEKRKTIHMAFHNLEKAFDRVPHNLIWLALRQRHVPEAYVSWIKLLYREATSSVRCVCMDTVTKDIQTRRPWTLLYANDVMIASETQHGLEQQVQEWKMRLERFGMKLNIQKTEYLECGDQTDGTIAAGGVQLNKVTQFKYLGSCVNSDCATLQDAKTRVSAAWMKWRQVTGVLCDKKIPLRLKSKVYRSVVRPVALYGTECWPTTKKHEQALHMMEMKMLRWTLGLTRLDHVMNEEVRKTPKVVPITEKMRELRLRWYGHVLRSNDTLMAETALELNVEGCRPRGRPFTRWLDRLKDNTRLAKVTCRDAADRTKWKNRCKQADSTQVGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA78</accession>
    <name>W5MA78_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA78"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005287"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005295.1">
        <property type="protein sequence ID" value="ENSLOCP00000005287.1"/>
        <property type="gene ID" value="ENSLOCG00000004427.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013867"/>
    <dbReference type="HOGENOM" id="CLU_026821_1_2_1"/>
    <dbReference type="InParanoid" id="W5MA78"/>
    <dbReference type="OMA" id="EYMANEG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004427">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2760:FF:000004">
        <property type="entry name" value="HD domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="45"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="47762" checksum="7D69C65E877EBDEF" modified="2014-04-16" version="1">DPIHGHIEMHPLLVKIIDTPQFQRLRFIKQLGPCSFVYPGATHTRFEHSIGVSYLAGRLVEVLQDRQSELKITKKDKLCVQIAGLCHDLGHGPFSHMFDAKFLLRIGKSENEHEKTSVKMFEHILQPKYIKVHKQLTRKKIEVSLIASAVCVSVEGRTDKEFLYEIVANKRNGIDVDKWDYLASVRDSYYLGMQNSFNHERLLKFVRVCEVEGGRQICTRDKEIFNLYDMFNIRFSLHKRALQHRVVNIIVHMITEAFVKAAEHITVQSSNGLYNLSTAWNISYNVFHEILNSTSEELKEAREILQRVMRRKLYKFVGETRSKPDAHKKQVRTKLVCCINHNKWGVIDLKNNSTKLCIENTKMDYGMGDNNPIDQVHFYFKNNPTKGVKMDKNQVTNILPINFSEQLVQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAF0</accession>
    <name>W5MAF0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ARF guanine nucleotide exchange factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004366">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01004367">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004368">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004369">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000005359.1"/>
        <property type="gene ID" value="ENSLOCG00000004389.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157108"/>
    <dbReference type="HOGENOM" id="CLU_000691_1_2_1"/>
    <dbReference type="InParanoid" id="W5MAF0"/>
    <dbReference type="OMA" id="EVMCAYI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004389">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
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    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="BREFELDIN A-INHIBITED GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
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    </dbReference>
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        <property type="entry name" value="DCB"/>
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    </dbReference>
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        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="12">
        <location>
            <begin position="741"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="249"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="376"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="684"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="378"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="431"/>
            <end position="446"/>
        </location>
    </feature>
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        <location>
            <begin position="694"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50190"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1905" mass="214642" checksum="0C5287D41275E394" modified="2014-04-16" version="1">MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKEETEKLSPPPGDTKAGSSTLPPVKSKANFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGSAPDSTAPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQQIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALTNHKITWSLASRKRERQKLLRRSIFSAQSVSFQEARQMEKERQRQQHLQQSPVSQHEPDSPRLRRPKEQPAKPPPQEPDGGAPPTASEAEKSAQEDPEPENGSEFCSAENEQTEADQATAAAESLSKHDAEVIDEEEAPNYEEKAQEIVQSILKEVVNTVVGDSGESAGGESAEGTPGSLEEEGGLGSDSEHVHANGIPGTPISASFAPSLPDDRLSVSSNDTQESGVTTGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKKTAGKNSWKNWMKSHELRSKVLSLQLLLSILQNAGPIFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRAGHELGMTPVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTSLGQEKPSEQETNETKHPETINRYGSINSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDRFNKEVMYAYVDQMDFQGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYDEIAGKKISMKETKELTIKSNKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASLCLEGIRCAIRIACIFAIQLERDAYVQALARFTLLTASSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKARYISGTVRSKEGPIAGTKELSSDEYVGLGLVGGNVDRKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVVRCIAQMVNSQAGNIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTNVFEKHFPATIDSFQDAVKCLSEFACNASFPDTSMEAIRLIRHCAKYVSERPQAFKDYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFEALNDVLLDDILSQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLETWDKTCNCMLDIFKTTIPHALLTWRPSGAENEPPAQVDISDKQLDSISQKSLDIQARADDQQSTGSMEKLSLENRRQSQFSVGSAVLEDGRQARTPTKAQEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDTLDADVHVDTQDQGMYRYLTSEQLFKLLDCLLESHRFAKAFNSSNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDECRREAWEEVQKQLLNVCSEAISYFLTLTSESHREAWTNLLLLFLTKVLKISDDRFKAHASRYYPLLCEIMQFDLIPELRAVLRKFYLRIGIVFKISQTPSQEDGDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAJ9</accession>
    <name>W5MAJ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate 5-kinase type 1 gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005408"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005416.1">
        <property type="protein sequence ID" value="ENSLOCP00000005408.1"/>
        <property type="gene ID" value="ENSLOCG00000004517.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159258"/>
    <dbReference type="InParanoid" id="W5MAJ9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004517">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF26">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="50"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="560"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="626"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="664" mass="73920" checksum="0504560046FCC32C" modified="2014-04-16" version="1">MDAGDTEADLKKAFITEMPSSSGQPGSGPGKKIGHRGVDASGETTYKKTTSSALKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFPDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSLFYVTRDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRMVVMNNVLPRVVRMHLKYDLKGSTYKRRASKKEREKSSPTFKDLDFMQDLQDGLMLDIDTYNALVKTLQRDCLVLESFKIMDYSLLLGVHNLDQAERERQTEGPGGSSDEKRPVAQKALYSTAMESIQGGAACGESIDTDDMMGGIPAVNGKGERLLLYIGIIDILQSYRLIKKLEHTWKALVHDGDTVSVHRPSFYADRFFKFMSTTVFRKNSSLKSSPSKKIRGPLTVPKYTGPGAAFSASQLPSERDENIYDLRGARSFPTLDDEGXPDHLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDSSEHPRYRRHTQSCGHAGSFFYPRRSHDEDLQTITVEVELKRHDSEPAVVPRPSLERSPKVVVEPDASSQLSESTSHASVEDEEDVPITDIYFFSDGGCWVYSPVLRRARRQAGSASSPPEDRSWVYSPLHYGTPPPSVSDGESETVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBF3</accession>
    <name>W5MBF3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulatory factor X-associated ankyrin-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBF3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005712"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005720.1">
        <property type="protein sequence ID" value="ENSLOCP00000005712.1"/>
        <property type="gene ID" value="ENSLOCG00000004761.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160753"/>
    <dbReference type="HOGENOM" id="CLU_000134_23_0_1"/>
    <dbReference type="InParanoid" id="W5MBF3"/>
    <dbReference type="OMA" id="EQYMTAV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004761">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000031">
        <property type="entry name" value="Ankyrin repeat, family A (RFXANK-like), 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017362">
        <property type="entry name" value="DNA-bd_RFXANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF4">
        <property type="entry name" value="DNA-BINDING PROTEIN RFXANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038034">
        <property type="entry name" value="DNA-binding_RFXANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="73"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="22881" checksum="902B6614DAEF6788" modified="2014-04-16" version="1">SPGVDGISESRSEEGQFRHSTTLTNRQRGNEVTARPATLDTLSVQQLAAQGEIVQLTEHLSKDSFLINKPDERGFTPLMWAAAFGEVAVVNLLLEKGADPHRLARERESALSLASSGGYTDIVNILLKRGVDINTYDWNGGTPLLYAVRGNHIKCVEALLGWGADLTVEADSGYSPMDLAVALGHKKVQQVIEAHILQLLKKKDVNRSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCR9</accession>
    <name>W5MCR9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SOSS complex subunit B2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleic acid-binding protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Oligonucleotide/oligosaccharide-binding fold-containing protein 2A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sensor of single-strand DNA complex subunit B2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Sensor of ssDNA subunit B2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Single-stranded DNA-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SOSS-B family. SOSS-B2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCR9"/>
    <dbReference type="FunCoup" id="W5MCR9">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006178"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006186.1">
        <property type="protein sequence ID" value="ENSLOCP00000006178.1"/>
        <property type="gene ID" value="ENSLOCG00000005133.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155812"/>
    <dbReference type="HOGENOM" id="CLU_102724_0_1_1"/>
    <dbReference type="InParanoid" id="W5MCR9"/>
    <dbReference type="OMA" id="AHGEQKN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005133">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000072">
        <property type="entry name" value="SOSS complex subunit B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF5">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="14">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="129"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="223" mass="23627" checksum="F4E82742214F1F9D" modified="2014-04-16" version="1">MSNNSNEALFLIKDVKAGLKNLNIVFIVLEIDAGRVTKTKDGHEVRSCKVADKTGSITISVWDELGSLIQPGDIIRLTRGYASMWKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNQEFLAQTNMQNKTAKAEQRSNSPTNIANSGTPVQTGNGSQQSYSTASPAPNSRDPASSFGGAGRANGRVPGNGSPSAPPGAPPGISKPSVTISNGRDPRRASKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDL8</accession>
    <name>W5MDL8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:ch211-231m23.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDL8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006477"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006485.1">
        <property type="protein sequence ID" value="ENSLOCP00000006477.1"/>
        <property type="gene ID" value="ENSLOCG00000005374.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQGM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203527"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_4_1"/>
    <dbReference type="InParanoid" id="W5MDL8"/>
    <dbReference type="OMA" id="MYFSSIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005374">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071398">
        <property type="term" value="P:cellular response to fatty acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15170">
        <property type="entry name" value="7tmA_FFAR2_FFAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013312">
        <property type="entry name" value="GPR40-rel_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822:SF8">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01904">
        <property type="entry name" value="GPR40FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="327" mass="37733" checksum="21986B15D9362C83" modified="2014-04-16" version="1">LETTTEMDLLALAVYIVTFLTGLPSNVLALYAFSLKVRLKPLPIDILLINLTVSDLIFLLFLPFKMAEAFYDQQWVLPNFLCSLSAFIFFSTIYISTLFLTAVSVDRYLGVAYPFTYKKKRHPLYVIMVCIFIWIFSSAHCSIVYITEHFRPENASDNYSLCYDDFTEEQLAILLPVRIELCVVLFFIPLIISAFCYLNFIHILNTLANINHKKKQRAIGLALGTLLVFILCFLPYNITHIVGYIHRKSPKWRRFVLLLSTFNACLDPIIFYFSSSAFQETFKKFFFIQCNCLVDLLLTFKGKMISTWSSNSSALVHSVGTHIEYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF73</accession>
    <name>W5MF73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with RAB1A and RAB10; in a GTP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01035518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF73"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007032"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007040.1">
        <property type="protein sequence ID" value="ENSLOCP00000007032.1"/>
        <property type="gene ID" value="ENSLOCG00000005818.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158674"/>
    <dbReference type="HOGENOM" id="CLU_018687_0_0_1"/>
    <dbReference type="InParanoid" id="W5MF73"/>
    <dbReference type="OMA" id="TEFPCEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005818">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000009">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000019">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064536"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00067477"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="400" mass="46741" checksum="FB097723DB58A0E8" modified="2014-04-16" version="1">SRSFWVSRIQEFRAVLSGEKIDLDALRELCFGGIPFEGGIQSLCWKILLNYLPLEQKLWDSFLKKQRELYTQFLKEMIIQPGIAKANLGLSREDVTLEDHPLNPNPESRWNNYFKDNEVLLQIDKDVRRLYPDMAFFQRPTDYPCLLILDPQNEYETLRRRVEQTTLKAQTVAKNRSGVTNVSSPGKSQNLYPSNEYEVLPNGCEAHWEVVERILFIYAKLNPGIAYVQGMNEIVGPIYYTFATDPNSEWKEHAEADTFFCFTNLMSENRDNFIKSLDDSQCGISYKMESVYSTLKEKDMELYLKLQEQNIRPQYFTFRWLTLLLSQEFLLPDVIRIWDTLFSDKDRFGFLTLVCCAMLILIRDQLLTGDFTVNMRLLQDYPISDVHTILKKAKELQDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFF9</accession>
    <name>W5MFF9_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Coiled-coil domain containing 148</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="EMBL" id="AHAT01028882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5MFF9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007126.1">
        <property type="protein sequence ID" value="ENSLOCP00000007118.1"/>
        <property type="gene ID" value="ENSLOCG00000005892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699039"/>
    <dbReference type="KEGG" id="loc:102699039"/>
    <dbReference type="eggNOG" id="ENOG502QV5F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="KLKKYWA"/>
    <dbReference type="OrthoDB" id="448087at2759"/>
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        <property type="component" value="Linkage group LG12"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in liver and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039902">
        <property type="entry name" value="CCDC148/CCDC112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549">
        <property type="entry name" value="MUTATED IN BLADDER CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="498"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="629" mass="75229" checksum="AF6810B1A1522E5C" modified="2014-04-16" version="1">MLSTYWSPIYEVGRHKFVDINTMSGRDLRMFITNHRVEDIERLTLRMKDGLGSKKYKPVDYQQLQAITEAKRFASANIELKIHKTLQAARMTKENTVTRQHRQVWCKEYARLMKAGQRLDLEIQKFLDEEGINNDFLFQMKDNELQLEKERSLFRADTVDPIWQLKDDLRSRLSEMCSYTSQHPLKTQSIQCDLILEQVEYVKKQQKDIIEKLDLECLSVEEEISATRTQAWLSSTEEIRSSLQQIPEEVLNLECPYPDLKESLLKEFQSLDEKYSSRLQNITERLNGLDRCCGWSETDHLIFQSIVDQYPHDMKDRRVLYMDMLHRLFPHKSRQELSDHERHWDWYRFTVEQKKAVLQGWTRDRTDLLMKAVMTLEEACSTYDEEVALRMDRRHQQKVCAELKEKLRQWRAHQEEVARLEGAIAARKQEEEEDRLRAKQQKDEARRNLQKEKIKRYYTQKQSRRDELERRDRQRLEELKGLLAEQARKDQERVQFRQDLLQQRRKEKESQALRLQEQEEERQRRLENLRNQVAVVADLDPVRLMGATKSSKARQNIGAEEEVALQRPLFHLYTYTDTEILSDQRVRIEQALRQAGLHNTFYAKDVLSNINPPRPPRRDMESTLFQNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFN7</accession>
    <name>W5MFN7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adhesion G protein-coupled receptor G4b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFN7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007204.1">
        <property type="protein sequence ID" value="ENSLOCP00000007196.1"/>
        <property type="gene ID" value="ENSLOCG00000005960.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="HOGENOM" id="CLU_002753_3_9_1"/>
    <dbReference type="InParanoid" id="W5MFN7"/>
    <dbReference type="OMA" id="KEETECH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005960">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF435">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
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    </feature>
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        <location>
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        <location>
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        <location>
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            <end position="356"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="155"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43536" checksum="B32618817808617F" modified="2014-04-16" version="1">MASKSRKSAKVVCTYYNDFPLFQGTNKSKILNSEVVGITVENEVISDLHDPVIITFHHYEPVPTNMSRKCVFWDMTKVKEDTWNDFGCVTKEKSKEETECHCNHLTYFAVLLEINEGPVQHLVALTWVTYFGCAVTSISCVILIVLYARQRYISLLTVTVFLFNSFLSLATLENKSLCTFVAFTQHYSLLCSFTWMSIEAYNAFRLVYNVLNFYVKNYIPILCLVGYGLPLVVVLVLVSIPDVYGLHRIRSAENTETLMCWITTPLVHYITNVSFFLVIFILNLAMLVYITNTIQKRGPWKENRIMLISVWGLTCLFGTTWGLAFLNFGALRVLAQIAFCIINSLQGFFLCVRFFLLRHTMRTRSLQDSTSGSVKQSMLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG63</accession>
    <name>W5MG63_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collagen like tail subunit of asymmetric acetylcholinesterase</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG63"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007372"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007380.1">
        <property type="protein sequence ID" value="ENSLOCP00000007372.1"/>
        <property type="gene ID" value="ENSLOCG00000006099.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157248"/>
    <dbReference type="HOGENOM" id="CLU_650464_0_0_1"/>
    <dbReference type="InParanoid" id="W5MG63"/>
    <dbReference type="OMA" id="LCLPLWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006099">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF861">
        <property type="entry name" value="ACETYLCHOLINESTERASE COLLAGENIC TAIL PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868124" evidence="5">
        <location>
            <begin position="22"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="59"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="458" mass="48181" checksum="AD9BE032225F928D" modified="2014-04-16" version="1">SSMVLIAVGFQFQLFFCYVLSQSSFMDNILSFPAALKSLDHKKKLNICCLLTPPPPPLFPPPPLLREHKSHNEKVNNNEDGGEKPTLPTCVPGPPGPSGPPGPQGPNGIPGLMGPKGEKGEIGRPGAKGRPGPPGLPGHQGPAGWPGPNGPKGEKGDPGLMGLPGARGPMGPKGMPGYKGEKGSRGDEGEQGLKGEKGNMGLPGMLGQKGEMGPKGEPGVSGHRGPTGRPGKRGKQGAKGDTGALGPIGPAGPPGPTGYPGPPGLPGSDTIKGHFMVGPKGEKGVPGLPGRCYCNQPVTVNNPPYEEPSLGVNYPRVPVIFVVNNEEELGRLNTNNALAFRKDQRSLYFKDIEGWLPIQLTPFQSIEYPPDPDGFCGDGIVQAYNGEDCDDGNKIVTDGCIKCKHAYCGDGYRYEGVEECDGKDFGYQTCKSYLPGSYGQLRCTSYCYIDSTNCKYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGJ5</accession>
    <name>W5MGJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="11">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01035498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGJ5"/>
    <dbReference type="FunCoup" id="W5MGJ5">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007504"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007512.1">
        <property type="protein sequence ID" value="ENSLOCP00000007504.1"/>
        <property type="gene ID" value="ENSLOCG00000006214.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="HOGENOM" id="CLU_021278_1_1_1"/>
    <dbReference type="InParanoid" id="W5MGJ5"/>
    <dbReference type="OMA" id="PQSTVCE"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006214">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="283" mass="32029" checksum="DF627F0A85FD8474" modified="2014-04-16" version="1">DYCRTAMAGVGLPAEPERPHHRPFLIGVSGGTASGKSTVCAKIMELLGQNEVDHHQRKVVIVSQDSFYRVLTSEQKAKALKGQYNFDHPDAFDNELMYQTLKDIVEGKAVEVPTYDFVTHSRLQDRITVYPADVVLFEGILVFYSQEIRDMFHMKLFVDTDSDVRLSRRVLRDMKRGRDLEQILSQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMVAINLIVQHIQDILNGDLCRWQRGAVHSDAQGRGYKRGLPEQGDVGNGVTTPPGKRILLEPSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHH1</accession>
    <name>W5MHH1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 13">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="10 13">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="10 13"/>
    <dbReference type="EMBL" id="AHAT01007885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007888">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHH1"/>
    <dbReference type="FunCoup" id="W5MHH1">
        <property type="interactions" value="1829"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007830"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007840.1">
        <property type="protein sequence ID" value="ENSLOCP00000007830.1"/>
        <property type="gene ID" value="ENSLOCG00000006479.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063646"/>
    <dbReference type="HOGENOM" id="CLU_010862_4_1_1"/>
    <dbReference type="InParanoid" id="W5MHH1"/>
    <dbReference type="OMA" id="MKCCHEM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006479">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
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    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
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    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
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    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 13" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="557" mass="60499" checksum="F1D75FA82490E0DA" modified="2014-04-16" version="1">GLLPGETVIKEGKASILFPSANEVFYNPVQEFNRDLTCAVLTEFVREQLSARGVRVEVPGEESRVVVHLAEDGASQGEARTAVVGECCEDGLRVLEGLAASGLRSVRFALEVPGLRSVTANDCSSRAVNLIARNALHNSVSHLVTPSHRDASMLMYEARGRKERYDVIDLDPYGSPAPFLDAAVQAVSEGGLLCVTCTDMAVMAGNCGETCYSKYGSVSIKAKYCHEMALRIILHSLDQRAGVYQRFIEPLLSVSVDFYIRVFVRVRTGQARVKSSASKQALVYNCTGCGSFHLQRLGKRTSQGNQMKYSAASGPPVGPNCEHCGYKHHLGGPIWAEPIHSPEFVQKVLSAVSANPSRFATSRRIEGILSVITEELEDVPLYYTLDSLSSTVHCNTPSLLQARSALLHAGHRVSLSHACKNALKTDAPASALWDIMRCWEKMNPVKREKLSETSPAFHILSTAPVCPACWTEDRPALLLSPTPHPAFTLLSLRALPHSPLSLPALPHCSLTRCPASLSSLSPLKSFLCKRFHQGTCTQGEQCCYSHVDQQGQSAAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIC1</accession>
    <name>W5MIC1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Semaphorin-6B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functions as a cell surface repellent for mossy fibers of developing neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010713">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008130"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008140.1">
        <property type="protein sequence ID" value="ENSLOCP00000008130.1"/>
        <property type="gene ID" value="ENSLOCG00000006724.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3611">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159170"/>
    <dbReference type="HOGENOM" id="CLU_009051_2_0_1"/>
    <dbReference type="InParanoid" id="W5MIC1"/>
    <dbReference type="OMA" id="PTPHVDY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006724">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000009">
        <property type="entry name" value="Semaphorin 6B isoform 3 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000028">
        <property type="entry name" value="semaphorin-6A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF10">
        <property type="entry name" value="SEMAPHORIN-6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868229" description="Semaphorin-6B" evidence="19">
        <location>
            <begin position="23"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="588"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="20">
        <location>
            <begin position="28"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="620"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="684"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="787"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="903" mass="99431" checksum="83AA5A044F5CAF16" modified="2014-04-16" version="1">TAMTTLAQPLILLPLLLQLAVAVFPEEMGPLSTAPVEAARRYPVFLGRPHRSAVRQEPLLIQRILQVNRTLYIGARDDLFRVDLDGVAGEEMFYSKKRTWEPNKNDIRVCRMKGKHEDECRNFMKVLLSRKEGLFICGTNAFNPLCANYTGDTLEMVGETISGMARCPYDPRHANVALFADGSLFTATVTDFLAIDAVIYRSLGDGPALRTVKHDSKWFREPYFVSAVEWGQHIYFFFREMAMEFSYLEKVVVSRVARVCKGDLGGSQRVLEKQWTSFLKARLNCSVPGDAHFYFNVLHSTSPVIRLHGRDTVLGVFSTPANSIPGSAVCAFDMQQLASVFEGRFKEQKFPESIWTAVPDELVPKPRPGACAVQGSRFNSSTTFPDEVLNFVKTHPLMDEAVPSIGQRPWIVRTMVRYQLSKIVVDTAAGPYRNHTLLFLGSDTGTVLKFLVHPQEDNRAAYSSVFLEEFEGFNPDKCGVDSPSARRMLAMSLDRPSRSLLLAFPACVVRVPLARCQQHARCMKNCIALRDPYCGWTRGSTCSFLEPGTRLPFEQDVEHGNTSHLGDCEGLLQESFVEEPEGLVSVNLLVLSAVSAFATGAALSGLAVCWFMRHQHRRRACSGGAGSSSSRRKGDKEQSMLPQGGGGSVVSVTRPCGGGDRPPPRGQSETLLAPLMVQNGWPKPVGGVMRLSQPDLDPGLLPTPEQTPLQQKRPPPGLRLSAGDPSWDQSQTYLNSAGTPCPPPSSVIYLSSKHLPTARGMRHPEEQGDPPQLAERHYLALAVHEPPPQLPLSREHGTRPGAPLRNSAGEYPYPVTPQDSPERRRVVSAPNTELDFGDSLRWNPETLNFNSNNASSSGRHHYASRAPPALGRAALHSTQGLGSLADFSKLLHKSASDRTPSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIY5</accession>
    <name>W5MIY5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lateral signaling target protein 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Zinc finger FYVE domain-containing protein 28</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lst-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199858.1">
        <property type="nucleotide sequence ID" value="XM_015344372.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIY5"/>
    <dbReference type="FunCoup" id="W5MIY5">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008344"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008354.1">
        <property type="protein sequence ID" value="ENSLOCP00000008344.1"/>
        <property type="gene ID" value="ENSLOCG00000006897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690870"/>
    <dbReference type="CTD" id="57732"/>
    <dbReference type="eggNOG" id="KOG1819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157217"/>
    <dbReference type="HOGENOM" id="CLU_007360_1_0_1"/>
    <dbReference type="InParanoid" id="W5MIY5"/>
    <dbReference type="OMA" id="CYVREVQ"/>
    <dbReference type="OrthoDB" id="20035at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006897">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15731">
        <property type="entry name" value="FYVE_LST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000092">
        <property type="entry name" value="Lateral signaling target protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043269">
        <property type="entry name" value="FYVE_LST2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051118">
        <property type="entry name" value="LST-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465:SF2">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="908"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="382"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="452"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="615"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="685"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="740"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="978" mass="106249" checksum="11B3F1A54CCB3E5C" modified="2014-04-16" version="1">MMNRFRKWLYKPKRTDPQLLAQFYYADEELNQVATELDSLDGRKDPQRCTLLVNQFRSCQDSVLNIINQIMDECIPADRANRDFCVKFPEEIRHENLGGQLWFGAECLAAGSIIMNREIESMAMRPLAKDLTRSLEEVRTITRDQALRDLNTYTDKMKEALRHFDSLFAEFELSYVSAMVPVKSPKEYYVQQEVIVLFCETVERALKLGYLTQDMIDDYEPALMFTIPRLAIVCGLVVYSEGPLNLDRKAEDMSELFRPFRTLLRKIRDLLQTLTEDELFTLERSLCISQDGEFPAVPTLPEVAVPPPAVVNPVPTPLAPPAVPKVPLQQEVPPSGPSAPGKEQQQSEQVEDADPELACSMQYDEQELEQLNMMVHRVGDEMSSLLSPPSLCQSPARRGACSGSSSTEASPRRGPAGRAGVDQGEEDRVFFMEDLDGAGEALAGIEDSPSLAWGGASCPSEPVPPGPALQRSPVQNGWPGGGSSVVEAALRQSQSCQSPDKPGLAGAPCPGPEAPEPPACLNGWEAGSEDAETAEMIAHRTGGMKLSATVIFNPKSPSLSDVTVEAAADKLLLLPQEPEPASPGPQKLSGLVATHCLLNSCVCCGSCEDSREDGLEASGDKGKCTPPSPVIHTSGSLAPSKDTATAAATTGIADSSGPLDAAEADSRLEQRSLRCVRCLDQASGTQAAPDRTPLGDAGACSHQRRAERPRQAGAGHDGRTQPAGGERELEDDGNLDSRSRSSSQDSSLSSISRSDCESVSVTTCSLSSTYTPSPVSSLTASSCTSEDLDHQEIQQALQAAKMASRDKIRSRFHSSSDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPEADDRDKQRKGQGRRSAALEDCALCQETVSSSELAAKARDGQFEDPPDWVPDEACSYCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQMKPVRVCTHCYMFHVTPFYSDKAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ27</accession>
    <name>W5MJ27_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Integrator complex subunit 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MJ27">
        <property type="interactions" value="1487"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008386"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008396.1">
        <property type="protein sequence ID" value="ENSLOCP00000008386.1"/>
        <property type="gene ID" value="ENSLOCG00000006927.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014184"/>
    <dbReference type="HOGENOM" id="CLU_007659_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJ27"/>
    <dbReference type="OMA" id="GRESKXY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006927">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056518">
        <property type="entry name" value="HEAT_Ints3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24566">
        <property type="entry name" value="HEAT_Ints3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="85"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Ints3-like C-terminal" evidence="8">
        <location>
            <begin position="470"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Ints3-like C-terminal" evidence="8">
        <location>
            <begin position="672"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="815"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="846"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="881" mass="97951" checksum="AD7442D490168465" modified="2014-04-16" version="1">KETQKRKPPSTVAGARQAARRAKMEPPSAKGRPQGRLLVSTSLDAKDELEERLDRCVGIVSSMIGGLSEREANDALTAHVCKGSQQHEEVCLGLFTLLLTEPTQAQRCYRDLTLVNRDGMNIVLMKINQILMEKFLKVQDICRTQLVWLVRELVKSGVIGADGVCMTLMKQIAGGDISNKNIWLAENVLDILVEQREWVMKSSMLIAMSVYTYLRLIVDHHGSPPLLALRQREVDFCIGLLREKFMDCFIIGRDLVRLLQNVARIPEMDLLWRDLLHNPQGLSPQFTGLSVCPLSSQSTGLSVCPSVCPLSSAAGRKLELRISATDGPMRLLCPVRVAPLLECFHSLCVCLTSTLSHSHLAPLFDNPKLDRELRAMLRDRFPEFCSSPSPPVEAVKMDESVSLEMENHVSDKEDGCYDNTEAAFSDDEEELNNKGRESKXYPLSSSVCLPGSLFPSLFQHHRGGAARPVALHSASDTEAQCEVMQEIVDLILEEDFDSEQMSALASCLAELFKGHFRGDVLPEEITEESLEESVCKPVCLIFRNLCQMQEDNSGFSVLLDMLAELYQKQPKIGYHLLYYLKASKAAVGRMSLYESFAQATALGDLHTCLMMDMKACQEDDIRLLCYLTPSIYSEVCLSVSLSLCLSVSHTALYPTLCCLSLHVLMSTVPALAEHPEALSCLLLQLRREKPSEEMVKMVLSRPCHPEDQFTTSILRHWAAKHDDLLAEHIKALLIKNNSLPPSLRLSLRSSSSKLAQLTLEQILEHLDNLRLSLSNTKHNFFSQTPILQALQHVQASCDEAHKMRFSDLFSLAEEYEDSSSKPPKSRRKAAVSSPRSRKGAAPPASNEEESGSSSASEEEDSKPKAPKRKRKGSSAVGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ33</accession>
    <name>W5MJ33_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 25">P2X purinoceptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">P2X purinoceptor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4. Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology. Could also function as an ATP-gated cation channel of lysosomal membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="25">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Functional P2XRs are organized as homomeric and heteromeric trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Membrane</location>
            <topology evidence="25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21 25">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ33"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008392"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008402.1">
        <property type="protein sequence ID" value="ENSLOCP00000008392.1"/>
        <property type="gene ID" value="ENSLOCG00000006936.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSUI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230351"/>
    <dbReference type="HOGENOM" id="CLU_034469_2_0_1"/>
    <dbReference type="InParanoid" id="W5MJ33"/>
    <dbReference type="OMA" id="NNCVPGY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006936">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.940:FF:000005">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.940:FF:000010">
        <property type="entry name" value="P2X receptor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003047">
        <property type="entry name" value="P2X4_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF18">
        <property type="entry name" value="P2X PURINOCEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01311">
        <property type="entry name" value="P2X4RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 25" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 21 25" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 21" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 21 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21 25" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="291"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="24">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="114"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="385" mass="43972" checksum="8E6D26D1190313FB" modified="2014-04-16" version="1">CFSMTQCFFEYETQRIVVIRSRKVSLINRLIQLAILSYVIGWVFVWKKGYQEFDSIISSVTTKVKGIALTNASHQGLRVWDVADYVIPPQEETSFFVMTNLIITPNQTQGSTKCIHLRDNSTICTSDSNCTAGFTDVHSNGVQTGKCVNFSGDVRTCEVRAWCPLENDLNLPRPALLKDAENFTVLIKNSIRFPKFNFNKRNILPQVKTSYLKQCIFNRVTDPNCPIFRLGDIVREAQEDFQKTAVEGGVMGIQINWDCDLDQSQNKCVPIYSFRRLDNKDPENNVAPGYNFRFAKYYKGPGEEESRTLIKAYGIRFDVMVFGKAGRFNIIPTMINIGSGLALLGVATVLCDCIVLTFMKKKTFYRLKKYKYVDDYERVNSTTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ96</accession>
    <name>W5MJ96_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="13">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640323.1">
        <property type="nucleotide sequence ID" value="XM_006640260.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ96"/>
    <dbReference type="FunCoup" id="W5MJ96">
        <property type="interactions" value="1633"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008455"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008465.1">
        <property type="protein sequence ID" value="ENSLOCP00000008455.1"/>
        <property type="gene ID" value="ENSLOCG00000006978.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696995"/>
    <dbReference type="KEGG" id="loc:102696995"/>
    <dbReference type="eggNOG" id="KOG3172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086153"/>
    <dbReference type="HOGENOM" id="CLU_099537_1_0_1"/>
    <dbReference type="InParanoid" id="W5MJ96"/>
    <dbReference type="OMA" id="HTITCET"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006978">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="127" mass="13973" checksum="CB28B868D39F9EBF" modified="2014-04-16" version="1">MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGGMGRGNIFQKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJK0</accession>
    <name>W5MJK0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJK0"/>
    <dbReference type="FunCoup" id="W5MJK0">
        <property type="interactions" value="1148"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008559"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008569.1">
        <property type="protein sequence ID" value="ENSLOCP00000008559.1"/>
        <property type="gene ID" value="ENSLOCG00000007070.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267181"/>
    <dbReference type="HOGENOM" id="CLU_075666_2_1_1"/>
    <dbReference type="InParanoid" id="W5MJK0"/>
    <dbReference type="OMA" id="HDISNRI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007070">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="136" mass="14937" checksum="0E548557A02806CC" modified="2014-04-16" version="1">MPEPAKSAPKKGSKKAVTKTAGKGGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVSDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKMNVKIVHQSAVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJQ8</accession>
    <name>W5MJQ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox D10a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636556.1">
        <property type="nucleotide sequence ID" value="XM_006636493.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJQ8"/>
    <dbReference type="FunCoup" id="W5MJQ8">
        <property type="interactions" value="473"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008617"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008627.1">
        <property type="protein sequence ID" value="ENSLOCP00000008617.1"/>
        <property type="gene ID" value="ENSLOCG00000007118.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686774"/>
    <dbReference type="KEGG" id="loc:102686774"/>
    <dbReference type="CTD" id="30404"/>
    <dbReference type="eggNOG" id="KOG0487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158266"/>
    <dbReference type="HOGENOM" id="CLU_057871_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJQ8"/>
    <dbReference type="OMA" id="DNWTDPN"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007118">
        <property type="expression patterns" value="Expressed in embryo and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046333">
        <property type="entry name" value="HXA10/ABDB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="261"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="37879" checksum="747D6FEBB02FA3CB" modified="2014-04-16" version="1">MSFPNSSPAASTFLVDSLIGACRTDSFYSSSNMYMSSSTEMGSYGMQTCGLLPSLGKRGEVSHQNMGMNVHSYIPQIDNWADPSRSCRIEQPTTQQMPTCTFPPNIKEESNCCMYSDKRAKVNSSEIPAYPSLIPESCSVDSPEIPVPGYFRLSQTYASGKNQEYSNASETSSNTILQLSRVNPKSQLSTLAEVQKKITEIPNNRDTAKTPTPVQSPEPKSSSQEEKNCSTEASASSPEVPDKDAKECKAEAPTSNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKSVNLTDRQVKIWFQNRRMKLKKMSRENRIRELTSNLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJS0</accession>
    <name>W5MJS0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RasGEF domain family, member 1Ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220582.1">
        <property type="nucleotide sequence ID" value="XM_015365096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJS0"/>
    <dbReference type="FunCoup" id="W5MJS0">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008629"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008639.1">
        <property type="protein sequence ID" value="ENSLOCP00000008629.1"/>
        <property type="gene ID" value="ENSLOCG00000007128.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694069"/>
    <dbReference type="CTD" id="327572"/>
    <dbReference type="eggNOG" id="KOG3541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155816"/>
    <dbReference type="HOGENOM" id="CLU_022907_2_0_1"/>
    <dbReference type="InParanoid" id="W5MJS0"/>
    <dbReference type="OMA" id="TWAKVQS"/>
    <dbReference type="OrthoDB" id="20825at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007128">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000008">
        <property type="entry name" value="Ras-GEF domain-containing family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000007">
        <property type="entry name" value="Ras-GEF domain-containing family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF197">
        <property type="entry name" value="RAS-GEF DOMAIN-CONTAINING FAMILY MEMBER 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="42"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="212"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="480" mass="56138" checksum="379023F8F5F414D4" modified="2014-04-16" version="1">MPLSSEENMPQTPPFVAMFSTSGYNRNLYQTNEDSYGGLYYHDNNLVSGSVEALIQLLVPNVDYYPDRTYIFTFLLSSRLFIHPYELLAKVCHLCIEQQRLCDPGADKIRLRKIAPKVLQLLTEWTDTFPYDFRDERIMRSLKELTHRLLTGDEIYRKAVQQMTQSLIRKLTTLSQYEEVLLKINATVPDRLTVLKTKPQSIQRDIVTVCSDPYTLAQQLTHIELERLSYIGPEEFVQAFVNKDPLDNEKSCFNDHKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEYFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWGKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSLTAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHINFEKFWELAKQVSEFMTWKQVECPFERDRKILQYLLTAPVFNEDALYLASYESEGPENHIEKERWKSLRSSLLNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK47</accession>
    <name>W5MK47_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NOC2-like nucleolar associated transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK47"/>
    <dbReference type="FunCoup" id="W5MK47">
        <property type="interactions" value="1654"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008756"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008766.1">
        <property type="protein sequence ID" value="ENSLOCP00000008756.1"/>
        <property type="gene ID" value="ENSLOCG00000007227.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010057"/>
    <dbReference type="HOGENOM" id="CLU_011272_1_2_1"/>
    <dbReference type="InParanoid" id="W5MK47"/>
    <dbReference type="OMA" id="GCLRYYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007227">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="643"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="699"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="773" mass="88219" checksum="77B43EB282B26226" modified="2014-04-16" version="1">MQRKLEDLSVDEFLESGFDLEDDEDYEEEMAAGQEGPSKRSADPTAASQDRRVGKKGKASEHKEQLSRLKSKDPEFYKFLEDNDRKLLNFDGSDSSEDEEDGKYHKLPAHLEEASSDEGDEDVKPAGKSKKSADVIKVTEKMVEEWKAGIKKELSPRLFREVTQAFKAAVATTQGGGGDPGRYKVEDGSVFNALVSFCIKDLFLPLQKMLLLKPNKDKMKLVLPSSSPRWQKAQTDVKMYLAGLVQQLLSCLTEASVISAVLKHANQMVPYYLCLPKQCRHFLKQLIKQWSTGEETVRVLAFLALNKICRHKQGSYLSSVLKHMYIAYVKNCKFTSPNVLPVINFMQRTLTEMYALDTQESYQQGFIYIRQLAIHLRNAMTMKKKETYQSVYNWQYVHCLYLWCRVLGTLHPSDVLEPLIYPLCQVIVGCVKLVPTIRYYPLRMHCVRALSLLSESTKTFVPVVPFLLEVFQQVDFNKKPGRVSVKPINFAVILKLSNTNLQEKAFRDGLIEQLYDLLLEYFHTQACSIGFPELALTTVVQLKAFLKECKVANYCKQIRQLLEKIQENSAHITSRRQRATFGVADRVAVEAWEKQIAEEGTPLTKYYSHWKKLREKEIQLEISGKERMEDLNLPEIKRKKLHERKEEDKKEFKDLFESDSASDDESVGFKVKAKQQKTAGAREASDDDDFLADLSDMSSEGEEGGEGEYEVEGDSDEEDAFGEDDADEEEEKNQPSKGKAKARPPKKLSQRDLEELGHGDEDMVEDLVLSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKG4</accession>
    <name>W5MKG4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Progestin and adipoQ receptor family member IIIa</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626814.1">
        <property type="nucleotide sequence ID" value="XM_006626751.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKG4"/>
    <dbReference type="FunCoup" id="W5MKG4">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008873"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008884.1">
        <property type="protein sequence ID" value="ENSLOCP00000008873.1"/>
        <property type="gene ID" value="ENSLOCG00000007313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695124"/>
    <dbReference type="KEGG" id="loc:102695124"/>
    <dbReference type="CTD" id="393683"/>
    <dbReference type="eggNOG" id="KOG0748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155291"/>
    <dbReference type="HOGENOM" id="CLU_023075_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKG4"/>
    <dbReference type="OMA" id="HSQPCPD"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007313">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF15">
        <property type="entry name" value="PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="36197" checksum="D257ECC305B8090A" modified="2014-04-16" version="1">MPQKLLKSAHYIELGSYQYWPVLVPRGIRLFTYEQIPVFLKENPYITDGYRAYLPSRLCLKSLFILSNETVNIWSHLLGFLLFFTLGVYDMIAVLPTAGASGEDYVIYSIGLFCFQVCMLCSVGYHLFCCHRSEKTSRRWMALDYAGISIGILGCYVPGVFYAFYCNNYWRQVYLITVLAMILAVFFAQIHPHYLTQQWHRLRSIIFCSVAGYGVIPTIHWVWLNGGFSALIVQAFVPRVLVMYFIAALAFLFYISKVPERYFPGHLNYLGSSHQVWHILVVLMFYWWHQTAVYIMQYRHSQPCPDLSVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML58</accession>
    <name>W5ML58_LEPOC</name>
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        <submittedName>
            <fullName evidence="5">Excision repair cross-complementation group 4</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML58"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009117"/>
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        <property type="protein sequence ID" value="ENSLOCP00000009117.1"/>
        <property type="gene ID" value="ENSLOCG00000007513.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004394"/>
    <dbReference type="HOGENOM" id="CLU_002265_1_1_1"/>
    <dbReference type="InParanoid" id="W5ML58"/>
    <dbReference type="OMA" id="MENVDIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="442"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="488"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="637" mass="71840" checksum="FD7C1A25B5C89426" modified="2014-04-16" version="1">MTGPLLEFETEMFLSLFGSDGLLVTAEGLGLDRILLQFLRVYSEEGSLVLLLNTTTPEQEFFTEKLRAEGVSHLPRTVTSEVPSGERLDVYTRGGVLFVTSRILVVDFLTDKIPASLITGILVYRAHKIIESCQEAFILRLFRQKNKTGFIKAFTDKPTAFSSGFCQVERVMRNLFVRKLFLWPRFHAAVNSVLDRHKPDVVELRVSLTPGMSAIQSSVLDIMNACLKELKRYNPTLEVEDLSLENALGRSFEKTIRHYLDPLWHQLGAKTKSLVQDLKILRTLLLYLTQYDCVTFLNLLESLRSSQKAFGSNSGWLFLDSSTSMFVNARSRVFRTLEPKDGLKKKAGATTNQEQNGEAAEVKKELVLEKSPKWEALTEVLQEIEKENNSSELGPGRVLICASDDRTCAQLQEYVTRGPEALLNRLYSRTFGRKETLLELNGEKRVPAKAKGKRGAEGGTKAKKPRAQAGKKQSKKPALTLTQMVGKGDSREDGPAEREPMGSSADEEEGMPEEEEGEEKEEEEEGLILNLSSDSYYGVMKEPLTVIHPLLGCSDPYSLTRVLHEVEPRYVVLYDAELPFVRQLEVYKASRPGQPLRVYFLIYGGSTEEQRYLTALRKEKQAFEHLIRTTESFLLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLX6</accession>
    <name>W5MLX6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin regulated spectrin-associated protein family, member 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009385"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009396.1">
        <property type="protein sequence ID" value="ENSLOCP00000009385.1"/>
        <property type="gene ID" value="ENSLOCG00000007726.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685004"/>
    <dbReference type="CTD" id="562741"/>
    <dbReference type="eggNOG" id="KOG3654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182975"/>
    <dbReference type="InParanoid" id="W5MLX6"/>
    <dbReference type="OMA" id="KAPQPMG"/>
    <dbReference type="OrthoDB" id="2125658at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007726">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAMSAP"/>
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        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
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    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF1">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF08683">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="222"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1352"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="793"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="903"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1167"/>
            <end position="1209"/>
        </location>
    </feature>
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        <location>
            <begin position="1226"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1313"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="747"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="862"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <end position="531"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="718"/>
        </location>
    </feature>
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        <location>
            <begin position="793"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="822"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="921"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="948"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="993"/>
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        </location>
    </feature>
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        <location>
            <begin position="1007"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1337"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1492" mass="167491" checksum="0F431F8BF646E5AB" modified="2014-04-16" version="1">MGDVADSKEIKKTFIVPAIKAFDHYDFTRAKIACSLTWLVAKAFGTDNVPEDLKEPFYTDQYDQEHIKPPVVNLLLSAELYCRAGSLILKSDAAKPLLGHDAVIQALAQKGLYVTDQEKLVTERDLRKKPIQMSAHLAMIDTLMMAYTVEMVSVEKVVACVQQYSPFFPDSDMPYDTEDAVTSWINKVNEHLKDIIIQEQKMRESQCSDIAGGPRSPTKWYWKLVPARYRKEQALPKQMPCIPPVDNLLKDSTDGSALAALIHFYCPEIVKLEDICLKETMSLADSLYNLQLIQEFCQENLNRCCHFTLEDMLYASSSIKNNYLVFMAELFWWFEVVKPSFVQPRVVDTEASQPVHSLRNMPSVPISNATKRSFIESPSSSDQPSLPLSPSESLMSVRHTQPQSQPPVSGVMKRSTSMSYVDGYVGTWPKEKRSSAHGVSFDIPFDNENTMQTPTAPSRGMTRSISTEGLGFKVHQMPKNLIKRNLSFQPVNGQKEKEGIEEEECPESLTGTELSSKHSGRGNNIRNQHGLSNGPVMDGHGNLTGTPSIEEALKIIHNTEKTHTLLQSDKVNNGFFLHCQDLEDSSSQPKLEEAGIDSVTEIKGAMSPDTTEVDTGIHVQTEDIQETMDEDSSLRDYTVSMDSDMDHDYELKAGNPREMISPCPSSLSAKSQAGSTASSSSGIKMTSFAEQKFKKLNNVDGRSSGSSSQKTTPESSELNIPHMVAWAQTPEESPVRQTGRDPTQLLASEMVQLRMKLEEKRRAIEAQKKKVEAAFTRHRQRMGRTAFLTVVKRKGDGTSPLREEAAGSEDEKLSTDSQPFKTADDNNLRPEKCKTDITDSMDQAQNRWLKSPNDENLGEVDLVEYTKSIEKLNASLNFLQQEMQRLAQQQEVIMQMREQQAWVISPPQPSPQKQIRDLRTATRSTGSLSPVLSSGDSPRTSHRSPTSIKRKSASFHSKTPRTPRPNELKITPFNRILTPPQSVDSLPRLRRFSPSQTQTSSFVCLGHDSRPSSETEAREKDAAERLKPDHSFPKEGSEKGVEAEPPSTAEEPKEKPTKEREEEEREIKPLESTVSEVLSQPITETFIVTPTENPGDVLGQSTKSLIEVPLSILKPLEGQELEDHGEGEVSGEVYDEDQKMCCGFFFKDDQKGEDDMAVKRAALLEKRLRRERETQQRKQQLEAEMEQKKEEARIKAEEERQKKEDEKARREFIKQEYLRRKQLKLMEDMDTVIKPRPASSKQKKPRPKSVHRDIMESPKTPARAPAVSSLSLASLNLADTDSVQSGKRTPRSSDIASGNLYYLLKSTKLKNTRPESADGFLSPCRSGSRNGEKDWENGSTTSSVASNTEYTGPKLYKEPSAKSNKHIIQNALSHCCLAGKVNEGQKNKILEQMEKSEANNFLILFRDSGCQFRSLYTYCPETEEINKLAGIGPKSITRKMIEGLYKYNSDRKQFSHIPAKTMSASVDAITIHSHLWQTKKPVTPKKVLPSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM55</accession>
    <name>W5MM55_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 3-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.7" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01034731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM55"/>
    <dbReference type="FunCoup" id="W5MM55">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009464"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009475.1">
        <property type="protein sequence ID" value="ENSLOCP00000009464.1"/>
        <property type="gene ID" value="ENSLOCG00000007785.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102689642"/>
    <dbReference type="eggNOG" id="KOG4459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159164"/>
    <dbReference type="HOGENOM" id="CLU_017820_0_0_1"/>
    <dbReference type="InParanoid" id="W5MM55"/>
    <dbReference type="OMA" id="KQCWREP"/>
    <dbReference type="OrthoDB" id="8517835at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007785">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019797">
        <property type="term" value="F:procollagen-proline 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000003">
        <property type="entry name" value="Prolyl 3-hydroxylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039575">
        <property type="entry name" value="P3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049">
        <property type="entry name" value="LEPRECAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049:SF15">
        <property type="entry name" value="PROCOLLAGEN-PROLINE 3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="10" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868412" description="procollagen-proline 3-dioxygenase" evidence="16">
        <location>
            <begin position="35"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="17">
        <location>
            <begin position="563"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="758" mass="84699" checksum="3DC1C10EC5A04A6C" modified="2014-04-16" version="1">MAFRGGNTAASDVAPGSPLLSLLLLLLLSLSARAAGALLEPVGGQLLPYDRLYYSGVRAYFTQEWDRAAEFLERSIATRAELLQVRRGCHDQCEAAAAGDSARLDSADGSLWDLAVFGLVQRRAECLKFCLEQRVTPSAIPPVSLDIQYEFTNRNPYNYLQVTYYKLGRLEKAVSAAQTFFVSNPSHLEMRNNLEKYRRMKGVREEAFFDREKERHWTLHDSGVLAESISDWPRAAEAWRECVNETLAATEDCRANCAGEALQPSEEDNQLTGTKGLYERAAGLSLQLLSCRQSCVSLVATRPGRISPIEDFLPSLLEHLHLAEFKAGALQGAVMSVRAYLLFYPEDPETLSNLQLYLRTPGGSTPESAQPREDISRYVHRSLLEKKLLYFAMENLGFKFTDPDLWTPESVIPESLHESWRAEKEKLDERQAEGQREGETDDSGFYAGGPITLPGVSITMDDATLNGTNRVVLDGVLSPEECRTILGLATAAASSGDGYRGRRSPHTPHEKFQGLTVLRALRLSQDGLVSAAEAQLLYDLGERVRSLLQSYFRSPSPLYFSFTHLVCRTAVTGDQEGRTDLSHPVHADNCLLEPDTRQCWREPPAFTHRDLSALLYLNDDFEGGDLFFTDRDAKTVTAVVSPQCGRLVGFSSGPVNPHGVSAVTSGRRCALALWFTLDRLHRDMVSRKQCAGLHHRPENHKAMDHTTLRAYTQQRHDQTDYFSVQSVHQPVVHCRGCAEFRKTINNTDHYVIMYQGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPW6</accession>
    <name>W5MPW6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPW6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010425"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010439.1">
        <property type="protein sequence ID" value="ENSLOCP00000010425.1"/>
        <property type="gene ID" value="ENSLOCG00000008573.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163144"/>
    <dbReference type="HOGENOM" id="CLU_157356_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPW6"/>
    <dbReference type="OMA" id="HWISTVL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008573">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF12">
        <property type="entry name" value="PARVALBUMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="63"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="111" mass="11945" checksum="13B93BDA4E1B3BEA" modified="2014-04-16" version="1">MAFAGILKDEDVAAALQACQAADSFNYKTFFAKVGLAGRSAEDVKKAFAVLDQDKSGFIEEDELKLFLQNFKADARVLTDAETKAFLAAGDSKLKLIFSPPCSEFAALVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPX1</accession>
    <name>W5MPX1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Alpha-aminoadipic semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="14">1.2.1.3</ecNumber>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="25">1.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Aldehyde dehydrogenase family 7 member A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Antiquitin-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Betaine aldehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Delta1-piperideine-6-carboxylate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(E)-4-hydroxynon-2-enal + NAD(+) + H2O = (E)-4-hydroxynon-2-enoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58968"/>
            <dbReference type="ChEBI" id="CHEBI:142920"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(E)-non-2-enal + NAD(+) + H2O = (E)-non-2-enoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69767"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:142592"/>
            <dbReference type="ChEBI" id="CHEBI:143908"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:69768"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:12309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:16186"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>betaine aldehyde + NAD(+) + H2O = glycine betaine + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15305"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15710"/>
            <dbReference type="ChEBI" id="CHEBI:17750"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:15306"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hexanal + NAD(+) + H2O = hexanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17120"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:88528"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:67277"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nonanal + NAD(+) + H2O = nonanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69759"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32361"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:84268"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:69760"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>octanal + NAD(+) + H2O = octanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44100"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17935"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44101"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 32">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="14"/>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.8" evidence="25"/>
    <dbReference type="EMBL" id="AHAT01002751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPX1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010430"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010444.1">
        <property type="protein sequence ID" value="ENSLOCP00000010430.1"/>
        <property type="gene ID" value="ENSLOCG00000008570.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154938"/>
    <dbReference type="HOGENOM" id="CLU_005391_1_2_1"/>
    <dbReference type="InParanoid" id="W5MPX1"/>
    <dbReference type="OMA" id="DAWKVYM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008570">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008802">
        <property type="term" value="F:betaine-aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07130">
        <property type="entry name" value="ALDH_F7_AASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000018">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000015">
        <property type="entry name" value="alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF1">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 32" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="33">
        <location>
            <begin position="65"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="active site" evidence="31">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00058360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00066671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00070398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00077960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00080350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00081296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="540" mass="58721" checksum="ED0D51E7F40DFF7B" modified="2014-04-16" version="1">AMQLIALTAVKHLALRRKVLFRGFRQTATMSTLLINQPKYSWLKELGLKEENAGVYNGAWGGKGEVITSYCPANNEPIARVRQATMAEYEETVNKAKEAWKVWADIPAPKRGEIVRQIGDALRKKIKILGSLVSLEMGKIYVEGVGEVQEYVDICDYAVGLSRMIGGPVLPSERPGHALIEQWNPVGLVGIITAFNFPVAVYGWNNAIALTCGNVCLWKGAPTTPLTSVAVTKIIAEVLEQNNLPGAVCSMVCGGADIGTSISKDERIDLVSFTGSTHVGKQVSLMVQERFGRQLLELGGNNAIVVFEDADLNLVVPSAVFAAVGTAGQRCTTTRRLLLQESIHDEVVEKIAKAYKQVRIGDPWDPSTLYGPLHTKQAVEMYKAAIEKAKEQGGTLVCGGKVIDRPGNYVEPTIITGLPHDAPIVHTETFVPILYVLKFKTEEEAFAWNNEVKQGLSSSIFTKDLGRVFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGRESGSDSWKQYMRRSTCTINYSKDLPLAQGIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSI6</accession>
    <name>W5MSI6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Notochord homeobox</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSI6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011345"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011361.1">
        <property type="protein sequence ID" value="ENSLOCP00000011345.1"/>
        <property type="gene ID" value="ENSLOCG00000009299.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0843">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154361"/>
    <dbReference type="HOGENOM" id="CLU_094819_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSI6"/>
    <dbReference type="OMA" id="YSIYCCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009299">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000378">
        <property type="entry name" value="Notochord homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF67">
        <property type="entry name" value="GNOT1 HOMEODOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="168"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="170"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="236"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="279" mass="31179" checksum="DFCEE76FF969F2F8" modified="2014-04-16" version="1">MPVWDRPRNIYLWIRTVSPTSSPGFGTMQVPAMPLGMYGYHLQSWAPVYPEYASNNSPVPKPSNGKSFTIDALLGTSEQPSATCERASPPHCGGNCQPAVPPLSLQPPLCPQMAPHLYTPSFLQSHSGYPVCYYPHLSYQEPCRGGFYTQADPHLSKNSVPSFKHKPGKSKRMRTIFTNLQLSRLEKEFARQQYMVGSERFVLASSLQLTEAQVKVWFQNRRIKWRKQTLEQQQAKLAKLGLASPEDSADSQQGSEGKQEETDGSEESVDELEVSSPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MST9</accession>
    <name>W5MST9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01010241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MST9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011448"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011465.1">
        <property type="protein sequence ID" value="ENSLOCP00000011448.1"/>
        <property type="gene ID" value="ENSLOCG00000009383.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172313"/>
    <dbReference type="HOGENOM" id="CLU_000384_33_7_1"/>
    <dbReference type="InParanoid" id="W5MST9"/>
    <dbReference type="OMA" id="DESSHYI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="192" mass="21492" checksum="D99C4A1C64052EB7" modified="2014-04-16" version="1">QSCHTSTKYPLPTVEELTSQISGSTIFTKLDLRQGYFQIPLHPDSQNLTTVVTHISVFRYTRVPFGLSSAPNCSQKMMSTIFTGIPGVVFYLDDNVVHGPTCASHDERLHKVFKVLHHNHLTLNREKCVLAAYTIEFVGFCLIAAGLTLLQSNIKEPTMPAQVASFLGMTAYYLWFLPHYSATTAQLQQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTD9</accession>
    <name>W5MTD9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEST proteolytic signal-containing nuclear protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in cell cycle regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with UHRF2/NIRF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="1455"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011648"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011665.1">
        <property type="protein sequence ID" value="ENSLOCP00000011648.1"/>
        <property type="gene ID" value="ENSLOCG00000009530.1"/>
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        <property type="component" value="Linkage group LG17"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="InterPro" id="IPR029169">
        <property type="entry name" value="PCNP"/>
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    <dbReference type="PANTHER" id="PTHR16523">
        <property type="entry name" value="PEST PROTEOLYTIC SIGNAL-CONTAINING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PEST PROTEOLYTIC SIGNAL-CONTAINING NUCLEAR PROTEIN"/>
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    <dbReference type="Pfam" id="PF15473">
        <property type="entry name" value="PCNP"/>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="149"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022059"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="167" mass="17761" checksum="7C9A652D33D17CA2" modified="2014-04-16" version="1">MADEKRTDEKLLDDGGPEEKGGKGVKTKTVSSSNGGGSNTRSADKRSAEEAAGDSMVQPAPPKVSKIGLSSQALKKPAPISIKLGASKPKESAPVLPAKKPALAAVFNEDEDSEPEEMPPEAKMRMKNIGSRDTPTSAGPNSFNKGKHGFSDHQKLWERKLKTHTDK</sequence>
</entry>
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    <name>W5MUF7_LEPOC</name>
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        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 13b</fullName>
        </submittedName>
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    <organism evidence="7 8">
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="KEGG" id="loc:102699150"/>
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    <dbReference type="GeneTree" id="ENSGT00940000154607"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
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        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
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    <sequence length="542" mass="59762" checksum="5B14949D9BA5364F" modified="2014-04-16" version="1">MSYFGQIIGAVGEFGTFQKRLLLAICLPNFFVAFHMFGQVFTGLKIQHHCNTDWILKLDRNLTQEEQWNLTIPRDKHGNYESCKMYTPVNWDIAFIKANGINSTTQCTNGWVYNSSGDTSTLVSEFNLVCDNSGLNEISQSVYMAGLLIGALVFGPLADRFGRRSIILVSLLLQLGFGVGTAFSPSIYVYIVLRFVIGMTISGIIINTFVLGTEWSGISKRSFITILSHGFFSVGLMLLAGIAYGVRNWRTLQLVLSAPAAVLCVYFWILPESARWLLTQGKKEEAKKLILEAARINKRKIPEALLDKMEAETTGKTGSMLDLYRIPYLRKITVVLSYVWFVNSLAYYGLGLNVGNFGLDIYLTQTLFGLVELPARLGAIPMIEKFGRKKCQSCILVFGGIACLFILAVPEGMPVAVTVLAVLGKFGIASSFSISYVYTAELFPTVLRQNGVGLTSMSARAAGIVAPLVRLLEVYHKAIPLVIYGVGPLVGGVLCILLPETLNTELPDHTHQLKTKSIECDGVETGKHQMEEIKTKVKSTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUW4</accession>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 128</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015344758.2"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000012194.1">
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        <property type="gene ID" value="ENSLOCG00000009955.1"/>
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    <dbReference type="eggNOG" id="ENOG502RZ1U">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TMEM128"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31134">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31134:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20479">
        <property type="entry name" value="TMEM128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="169" mass="19366" checksum="15CD7A70CA468C9C" modified="2014-04-16" version="1">MADQVVDCEFQALRNRFKHNAELLLHGNSLGTDIDNTPEDEDKKEKPLPRFNRHAVFWILASGTLTYYVDFFGVIKEDVHIKSWWFNIGLILLAICLALALFCILFLEWFCGMRDYDREYPAIAPITTAAFIAASGSFNIALWPVWSFFTPVILFTQFMGVVMFISLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV72</accession>
    <name>W5MV72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OFD1 centriole and centriolar satellite protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV72"/>
    <dbReference type="FunCoup" id="W5MV72">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012281"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012302.1">
        <property type="protein sequence ID" value="ENSLOCP00000012281.1"/>
        <property type="gene ID" value="ENSLOCG00000010040.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQR5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001798"/>
    <dbReference type="InParanoid" id="W5MV72"/>
    <dbReference type="OMA" id="SHNYSTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010040">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055289">
        <property type="entry name" value="OFD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063:SF1">
        <property type="entry name" value="OFD1 CENTRIOLE AND CENTRIOLAR SATELLITE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063">
        <property type="entry name" value="ORAL-FACIAL-DIGITAL SYNDROME 1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16045">
        <property type="entry name" value="LisH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="726"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="867"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="391"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="895"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="946"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1020" mass="117313" checksum="B727DDFC848EC678" modified="2014-04-16" version="1">SACRFNMSAAKEDVISPDEMRKRLYQTFKNRGVLDTLKTQLRNQLIQELKHPVLRGEVALKPVPVEADSVLVQASNSLVADHLRNVGYEYTLSVFYPECGLEKDKVFSTRDLLQLMKISPRSALYKSLTSSMQKDGTKGFLMKLLIELTDHHLHRERCDTDTQTDSTPQYRESLAEKLQMIDDQFAALHHQGNKWESLEAKLAEYRKEIEAQVQAEMNQQLQHFKEVELARLKLEEKEKSQKEVSELRREMERTYQIKSEALINREKNAIERLQKQQEIEEKEIYAQRQSLLKEIETVRNREAELKQRIEAFEMNCRLQDEKTKTTEEALRRRELAVKTIEDTYDQKLKNELLKYQLELKEEYLKRTEKVTEDEKRIKAESLHLRKELAVLDQKTEEQRRAIAEVKRLQVELDAAMSQISLLTQQTKLLKERLEETTDYPVLKRERLELQAQVRLITRHLEDTQEENRQFKAALRQPTAEYLALQTEVRKMENARKLDEEEFENQKQVLETQLQNEIERSAQLKAQLMECEERAEWLATHAEDVKMQLKQTQLALENEVFRNPKPSLVDRSVLDLTADRIVPPDLYVDGPVQKSRRVPGGSLFEPAAGLASTSQRHLRPRGSSLDPDTELVAGAKARIRELEKEAESLEEAYRNYQHRVIQAAALSAPPPGPQSPQQQHCVALSGISSASPSRVTFADDMLGSRELLSGQFNGHLLDSLSIFGGAETAGVQTPPERNSPRRLSSTPLSTSKRKSRRGTAEDKDHSHTASHHSLPEWQIPPRDHPSSSADISVLGSPPLKSTAREHARILQNPQELVLKFESSSVCVLCIYDMEQYHSDIPKQLESDVSHQSGENMNRHSVTTPEPAIATSQQDLESVEHQAAEQQDTEEEEEAERWELERKQREARRLQERQEAMEREQRELERLMEEEQQHREPSLKGPAGNDTASKDPEGEEADPRSKTDPLEKYMKIVMQNKERGQQEPSSSKEAREQESSIAEILSEKDESSSIAALSHDDADDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVJ6</accession>
    <name>W5MVJ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVJ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012405"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012426.1">
        <property type="protein sequence ID" value="ENSLOCP00000012405.1"/>
        <property type="gene ID" value="ENSLOCG00000010138.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182825"/>
    <dbReference type="HOGENOM" id="CLU_015718_0_1_1"/>
    <dbReference type="InParanoid" id="W5MVJ6"/>
    <dbReference type="OMA" id="EVRCGAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010138">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="56"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="255"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="456" mass="50723" checksum="F0159FEDEACE325E" modified="2014-04-16" version="1">KLSLLNTERECISIHVGQAGVQIGNACWELYCLEHGIQPDGNMPSDKTIGGGDDSFNTFFSETGSGKHVPRAVFVDLEPAVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTVGKEIIDLVLERVRKLSDQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVSEITNACFEPSNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGTDSVEGEEEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWF3</accession>
    <name>W5MWF3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor IIIB 90 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">B-related factor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Interacts with MAF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWF3"/>
    <dbReference type="FunCoup" id="W5MWF3">
        <property type="interactions" value="1475"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012712"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012736.1">
        <property type="protein sequence ID" value="ENSLOCP00000012712.1"/>
        <property type="gene ID" value="ENSLOCG00000010360.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010349"/>
    <dbReference type="HOGENOM" id="CLU_010293_2_3_1"/>
    <dbReference type="InParanoid" id="W5MWF3"/>
    <dbReference type="OMA" id="EPPCKVM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010360">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000012">
        <property type="entry name" value="Putative transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000001">
        <property type="entry name" value="transcription factor IIIB 90 kDa subunit isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="18">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="388"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="550"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="694" mass="76273" checksum="05BC64D21C1C464E" modified="2014-04-16" version="1">MSSRVCKNCGCTDIDIDQARGDAVCTGCGSVLEDNIIVSEVQFVENSGGGSSAVGQFVSSDGSGKAPVLGTGFHTSLGKESRAQTLQNGKRQIHNLGHQLQLNQHCMDTAFNFFKMAVSKHLTRGRKMMHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHMLEFGDKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVIGVVKVCEATLRKRLTEFEDTPTSQLTISEFMKVDLEQECDPPSFTAGQRKVKLQQLEHELAKKLDEVEGEISGYQDEIETELENSRPRLKGLYASYAKEDIQDDSLSLASSSLAGDDERDDEELEAAAQHLSKDFCHEVLPQSGGPGAGGEPGQASPAKQPLPLATLLGPLPTAASLGLTESLRECIATANENDGNPENGELDLNGIDDDEIDRYILNETEVKIKTELWTRENSDYLKEQKEKEERIAKEKELGIYKEQKPKRSLKKREPIRASTAGEAIEKMLEQKKISSKINYDVLRDLNSKGSGSSPSKRQEDSGDNGTGAKKTSSHRKTTSTSRALATPVSSVGKRLQPLISAQPCKKRATEQLFSSLPSAFSLLQTACSSNPPAPSDPSQPVVVESGPVQYTEEDVEEEEEEEEEPCVSALELMGANDYGYEVDDEDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZY8</accession>
    <name>W5MZY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Laminin subunit beta 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZY8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013947"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013947.1"/>
        <property type="gene ID" value="ENSLOCG00000011344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698331"/>
    <dbReference type="KEGG" id="loc:102698331"/>
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    <dbReference type="eggNOG" id="KOG0994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160731"/>
    <dbReference type="HOGENOM" id="CLU_001560_0_0_1"/>
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    <dbReference type="OrthoDB" id="8545473at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011344">
        <property type="expression patterns" value="Expressed in zone of skin and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043256">
        <property type="term" value="C:laminin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
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        <property type="term" value="P:basement membrane assembly"/>
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000073">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin subunit beta-1"/>
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    </dbReference>
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        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF268">
        <property type="entry name" value="LAMININ SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin_N"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868559" evidence="14">
        <location>
            <begin position="17"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="16">
        <location>
            <begin position="21"/>
            <end position="250"/>
        </location>
    </feature>
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            <begin position="251"/>
            <end position="312"/>
        </location>
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        <location>
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            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="376"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
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            <begin position="428"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="478"/>
            <end position="525"/>
        </location>
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        <location>
            <begin position="526"/>
            <end position="575"/>
        </location>
    </feature>
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        <location>
            <begin position="820"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1052"/>
            <end position="1158"/>
        </location>
    </feature>
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            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
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            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
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            <end position="407"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
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            <end position="490"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="538"/>
        </location>
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    </feature>
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            <end position="573"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1164" mass="128049" checksum="370EE00FA51ED0AA" modified="2014-04-16" version="1">MWILLHVVALSALALAQQDCSQGACYPSTGDLLIGRATQLKASSTCGMTGIEVFCTPLGQWKMKCCPCDSKNPYGQNAHQIQNVVAQAGPNRWWQSRKDVNPVSLQFDTDSKFLLHNLVLDFKGPRPEALVVERSLDFGRTWQPFLYLASDCRTSFPQISTSPPRSLQDVQCHTMPPLPPNSSYQDQTVHISPIRQVSAINLPQSQKIERLSGFTNLRLNLTRLGQPPRISNARAPSAFYALKKMQVQGSCFCHGHANRCVPTANQLPGIQVHSVCDCQHNTAGVNCERCADLYNDLPWRPAEERNPHQCKKCECNNHAQRCRFDPVVYEASRMVSGGVCENCQHHTTGRNCEKCTPFYYRNPQSTISRQDACLRCQCSVEGAERGGQCDPETGACFCKANVEGPRCDRCKAGYYGLSASDPQGCKKCSCSVVGSVREQPCDPLTGQCQCLPNFIGLTCDRCAPNYWNPFSRNGCEPCKCDPRNSYGTSCNQLTGQCECRPGFGGRTCTECPDNSYGDLRTRCLPCNCDPLGTVRAGCDKTTGACLCQLGVTGPRCDHCQRGHCDRYPSCPLCPSCFFSLDREMQGFSNTLDSLSKQAMTLPGSSDQDLGPRILGIESTLQQMRDRLPYPPPSDGQITEAISTLHRLRDQASRLNPDFFLVDQSTSLNRQIDSLQTELGSITLQYTSKRDSANRILNGIPTGALSTIQSAYQESADAIKKAEGTKVLVDQSASQRGDAAALEGQIQQGNKKALEKLNSQLATRPDLTPTAAQVCGSTRSTPCTPVQCDGQLCPGPSAPPCGNRATCTGALPLGNKAMADTEETRSRLRELSSKIQRAAAEIQNTQDSANRVRQSTDKLANQIKQARRDLDKDMQDTRDFVQQLREFLSDPDTDPEAIQTVSEEVLNTKLPQSLDALRSKVDEIRRIAAGLPDSRKVLESTGPELDHARELLRKAETARTQAVLMEENVNQVLDTLTSEESSLRSLETNVQDTMRIVDGVKNNINEIEGLLNPAQKTLGELAVQMDQTKPQLTELRDLVQSTQQLAQQAGGDVSRAQSEANEAGQELLLLEGQYKELMRKWSGGAGQSGGGASSTRLQELQQEANDLILQSMSMMRTLSEREGSLLEANEELLIKSRRLEGMEEELQKILDDIRQKARVLNTCQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0V8</accession>
    <name>W5N0V8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 47a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0V8"/>
    <dbReference type="FunCoup" id="W5N0V8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014267"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014296.1">
        <property type="protein sequence ID" value="ENSLOCP00000014267.1"/>
        <property type="gene ID" value="ENSLOCG00000011606.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159694"/>
    <dbReference type="HOGENOM" id="CLU_015166_16_1_1"/>
    <dbReference type="InParanoid" id="W5N0V8"/>
    <dbReference type="OMA" id="HICRLRY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011606">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000049">
        <property type="entry name" value="Solute carrier family 25 member 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 47"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="103"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="210"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="32778" checksum="2D6E836D011E6637" modified="2014-04-16" version="1">LEKGGRAMHVVDFLAGSVGGALGVVVGYPLDTVKVRIQTERKLLGAWQCIHTIAKKEGVNGFFKGLTMPLITVSISSSVTFGTYRNCLQCLRQVRCGSLEAPTSKSDIFLAGLVAGVAQVSVMSPADIVKVRLQCQTEPYRPVQGSGLLSKPKYHSPVHCLLTIAREEGMLGLYKGATALCLRDGPSFATYFLTYSVFCDWLTPAGKNQPEWWAVLVAGGCAGMCGWSIGTPMDVIKSRLQVDGVRKKRYNGIVHCISESIQKEGLTVLFKGLGLNCLRAFPVNMVVFATYEMVIQLLHKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N158</accession>
    <name>W5N158_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N158"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014367"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014396.1">
        <property type="protein sequence ID" value="ENSLOCP00000014367.1"/>
        <property type="gene ID" value="ENSLOCG00000011692.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156542"/>
    <dbReference type="HOGENOM" id="CLU_006855_7_1_1"/>
    <dbReference type="InParanoid" id="W5N158"/>
    <dbReference type="OMA" id="DYTEMYK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011692">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032328">
        <property type="term" value="P:alanine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015816">
        <property type="term" value="P:glycine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015820">
        <property type="term" value="P:L-leucine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF102">
        <property type="entry name" value="SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER SLC6A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01206">
        <property type="entry name" value="ORPHTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 9" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="569"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="682"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="730" mass="81289" checksum="44E362A99A61DC86" modified="2014-04-16" version="1">MPKNSKVTQREHSNEHVTESVADLLAHEEPLDYKSSALNVGGAVQQKGKETDDTLESAEERPAWNSKLQYILAQVGFSVGLGNVWRFPYLCQKNGGGAYLVPYFILLIIIGIPLFFLELAVGQRIRRGSIGVWNYVCPRLGGIGFSSLIVCAFVALYYNVIIGWSIFYFFQSFQYPLPWNECPLKRNGSHAIVEPECEKSSATTYFWYRQTLNISSSISDSGGLNWKMTVSLLAAWCIVCLAVIKGIQSSGKVMYFSSLFPYVVLICFLVRGLLLRGAVDGIAHMFTPKLEKMLEPQVWREAATQVFFALGLGFGGVIAFSSYNKQDNNCHFDAALVAIINFITSVLATLVVFAVLGFKANIMNEKCVVENAEKILGYLNSNVLSHDLIPPHVNFSHLSSADYAEMYGVIKTVREDDFEQLGLDACLLEDELNKKAVQGTGLAFIAFTEAMTHFPGSPLWSALFFFMLINLGLGSMIGNMEGITTPMVDTFKVRKEIFTVSCCLGAFFFGLIFVQRSGNYFVTMFDDYSAGLPLTIVVILENISVAWIYGTKRFMQDLTDMLGFRPYSFYFYMWKYVSPICLCVLVMATIIEMAISPPGYNAWIQELASERFQGYPPWALAMCFTLIAVAMLPLPIVFIARQFNLVSDGSNKLSVSYKKGCMMKDMSNLEDNDETRFILSKNPSEAPSPMPTHRSYLGPGSTSPLEMTSAAPNSRYGTGYLMATTPESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1K4</accession>
    <name>W5N1K4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1K4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014513"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014542.1">
        <property type="protein sequence ID" value="ENSLOCP00000014513.1"/>
        <property type="gene ID" value="ENSLOCG00000011799.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153342"/>
    <dbReference type="HOGENOM" id="CLU_005884_2_0_1"/>
    <dbReference type="InParanoid" id="W5N1K4"/>
    <dbReference type="OMA" id="HNHPVWD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011799">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000243">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF4">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="11">
        <location>
            <begin position="182"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="439"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="512"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="638"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="680"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="357"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="772" mass="85140" checksum="8C6E13681FD44900" modified="2014-04-16" version="1">CRKVELANRKSGKMAAVQDGPIEPESEKEIKTEILNDAAGNLAAATAVGGVLSSPPVSAAVGTPKPTSTPTEGKDQQTLLAVLQFLKKNKLVESAEILRREAGLLEESEDSQGPDAGGAGSGVGPSVEPDSGDATSLLSRVSCASTPSATPTPIPAKVTVAAGGGDNQPDVSVVLSAYSQQGDPALYQVYYSGLKKFIESVLDCHRAELSQVFYPLFVHMYLELVYNNHENEAKSFFEKFHGDQECYYRDDLRVLSGLTKKEHMKGNETLLDFRTSKFVLRISRDSYQLLKRHLQERQNNQIWNIIQEHLYIDIFDGMPRSKSQIDSMSGSLAGEARREANKAKVFYGLLKEPEIEVPLDDEDEEAENEEGKPKKKKPKKDSMGSKSKKQDPNAPAQNRIPLPELKDSDKLDKIMNMKEATKRVRLGPDNLPSICFYTFLNAYQGLTAVDFTDDSSLIVGGFADSTVRVWSVTPKKLRGVKSAADLSLIDKESDDVLERIMDEKTASESKILYGHSGPVYGTSFSPDRNYLLSCSEDGTVRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRMFAGHLADVTSTRFHPNSNYVATGSADRTIRLWDVLNGNCVRIFTGHKGPIHSLAFSPNGKFLASGATDGRVLLWDIGHGLMIGELKGHTDTVYALKFSRDGEILASGSMDNTVRLWDSVKAFDDIETDDFTAATGHIHLPENSQELLLGTYMTKSTPVVHLHFTRRNLLLAAGAYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1M8</accession>
    <name>W5N1M8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.1</ecNumber>
            <ecNumber evidence="3">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="4"/>
    <dbReference type="EC" id="1.1.1.284" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01017866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1M8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014537"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014566.1">
        <property type="protein sequence ID" value="ENSLOCP00000014537.1"/>
        <property type="gene ID" value="ENSLOCG00000011820.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164529"/>
    <dbReference type="HOGENOM" id="CLU_016132_1_0_1"/>
    <dbReference type="InParanoid" id="W5N1M8"/>
    <dbReference type="OMA" id="FELMIRG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011820">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001160">
        <property type="entry name" value="Alcohol dehydrogenase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF32">
        <property type="entry name" value="S-(HYDROXYMETHYL)GLUTATHIONE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="16">
        <location>
            <begin position="28"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="15">
        <location>
            <begin position="208"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="16">
        <location>
            <begin position="501"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="15">
        <location>
            <begin position="671"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="855" mass="93167" checksum="58BDDC84D087ECDC" modified="2014-04-16" version="1">VIQCKAAVAWEPGKPLSIEEVEVAPPKAHEVRIKIAASGVCHTDWTYVFEMTKVNQTRPYPMVLGHEGAGIVESVGPGVTKFTPGDTVIPLFLPQCGECECCLSPKTNLCDKNCCQLAVLRENTQQGVMADGTSRITCKGQQIYQFIGVSSFSEYTVVPDTSVTKIRADAPLDKVCLLGCGIPTGYGAALNTAKVEPGSSCAVFGLGAVGLAAVMGCKAAGASRIIAVDINKDKFEKAKVFGATEFVNPNNHSKPIQKVLAEMTKGGVDFSFECVGNVSVMLLILKNLPLSHVAKAGELVSLLAGMKCMKSLPRLFKLFPKIIFQKLCFQKYLKYELTNSKLLYKLLSQVWMNVACLTQMYNTLLCGLMQSILMLSLTGWKSVESVPKLVEDYMAKRLKLDEFVSHTLPLDRVNEAFDLMTSGKRFDCFLGNLMWPQEGFNVLAVPPLDFQMGSQMLPRKTSESFKIRICVQEIIKCKAAVAWEPNKPLSIEEIEVAPPQAHEVRIKVVATGVCHTDLYFLYEGNQKEGFPTVLGHEGAGIVESVGPGVTKFKAGDKVIPLFVSQCGKCKFCVNPKTNLCENSWSNERYQVMSDPTTRFSCKGKPILQFMGTSTFSEYTVVKDIAVAKIHNSAPLDKVCLLGCGISTGYGAALNTAKVEPGSSCAVFGLGAVGLAAVMGCKAAGASRIIAVDINKDKFEKAKVFGATEFVNPKDHSKPIQEVLAEMTKGGVDFSFECVGNVSVMFCIKGLFNKNLSLRSALESCVKGWGVSVLVGWTDKHDFSARPIQLISGRTWKGTFFGGWKSVESVPKLVEDYMAKRMKLDEFVSHNMPLDRVNEAFDLMTSGKSIRTVLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2L0</accession>
    <name>W5N2L0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heparanase</fullName>
            <ecNumber evidence="10">3.2.1.166</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>endohydrolysis of (1-&gt;4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan.</text>
            <dbReference type="EC" id="3.2.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.166" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01017247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2L0"/>
    <dbReference type="FunCoup" id="W5N2L0">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014869"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014898.1">
        <property type="protein sequence ID" value="ENSLOCP00000014869.1"/>
        <property type="gene ID" value="ENSLOCG00000012076.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004874"/>
    <dbReference type="HOGENOM" id="CLU_021823_0_1_1"/>
    <dbReference type="InParanoid" id="W5N2L0"/>
    <dbReference type="OMA" id="RMYLHCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012076">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060055">
        <property type="term" value="P:angiogenesis involved in wound healing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46145">
        <property type="entry name" value="HEPARANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46145:SF3">
        <property type="entry name" value="HEPARANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866916" description="Heparanase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="541" mass="60277" checksum="A0CE6CEE248A0CDC" modified="2014-04-16" version="1">DRKGNMVITVLVVVLFLGVNTANVESAREDVLRFALIKADLFQVVKKVNERFLSVTIDANLISEEKYVDILRSQKLKTLAKALSPGFLRFGGTKADFMIFSPRNDSIKQRDFKSTSEFQGNVCNRQNLPSILEERLKSEWALEATRLLEENDNLYKTIKFSETTVDTLYSFANCSGLHLIFGLNALLRTDSNKWDSENAEKLLGYCESKQYNMSWELGNEPNSFEKKAGIQVDGHQLGKDFIHLHDILRKSRLFHSSGLYGPDVGQPREHTKDLLGRFLEIGGKVIDSCTWHHYYVNGRDASLEDFLDPDVLDTLATKTEEVFRIVAESSPGKKVWLGETSSAYGGGALGLSDTYAAGFMWLDKLGMAAKLGIDVVIRQVLVGAGFYHLVDSNFDPLPDFWLSLLYKKLVGPEVLNTTAVTSQGSRTKRLRVYLHCSNRKGQNVEKGAVTLFALNLSKRTARIILPPQISNKTVDLFLLQPGEGRIDSRSVLLNGEVLKMVDEKTLPVLKGTPIAAGTPLQLPGLSFAFYVIKDAQVPACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4D8</accession>
    <name>W5N4D8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclin dependent kinase inhibitor 2B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4D8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015497"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015526.1">
        <property type="protein sequence ID" value="ENSLOCP00000015497.1"/>
        <property type="gene ID" value="ENSLOCG00000012588.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162423"/>
    <dbReference type="HOGENOM" id="CLU_000134_37_1_1"/>
    <dbReference type="InParanoid" id="W5N4D8"/>
    <dbReference type="OMA" id="SALQVMM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012588">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF8">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 4 INHIBITOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="125" mass="13553" checksum="4E54EB93DA6798F6" modified="2014-04-16" version="1">LTMTSVDQLASAAATGQTEYVLTLLQNGCDPNGTNRFGRTPIQVMMMGNTSVARLLLLYGANPNLQDVTTGMTPLHDAASGGFLDTVMILDQYHADRNITDNFNRRPVDLAKANGHEDVVDFLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4P2</accession>
    <name>W5N4P2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019039">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4P2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015601"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015630.1">
        <property type="protein sequence ID" value="ENSLOCP00000015601.1"/>
        <property type="gene ID" value="ENSLOCG00000012678.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158537"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_3_1"/>
    <dbReference type="InParanoid" id="W5N4P2"/>
    <dbReference type="OMA" id="KLHTHIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012678">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="198"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="390" mass="45174" checksum="551F375FEE47BC33" modified="2014-04-16" version="1">LLNQQRNKFYCSKEEKLCVFCENDKEALCSQCVGSESHRGHTVISAEKAVQVFRAEMQAKKNHLETLREDAKGRSVGRKSLSQLPFQSQNQASSREEKLTQLFERLQQFLLRTSIQLETELEDERKVKAQFIQNNIEVVNSITCDVSAQIQSLEEMNLQSTEKHIHPNAVSHFRNKKFDFPKQEKSQMINSEFYLGKVAYRLWKQAQAAKFTVHSVTVALDPETAHPELTLFEELTCVRYRGRRLRIPDFPSRFDQCPCVLGTPGFKDGRQYWEVRVWSKKEWDLGMATESANRKGEVRLDPAHGYYTIILREGGRYAACNSPQVPLRPPRQPTKIGIFLDYDRGRLCFLDANNMYPLYTFEHRFTETLFPFFSPGMVIDGQNVEPLKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4X4</accession>
    <name>W5N4X4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein inturned</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Inturned planar cell polarity effector homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inturned family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4X4"/>
    <dbReference type="FunCoup" id="W5N4X4">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015683"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015713.1">
        <property type="protein sequence ID" value="ENSLOCP00000015683.1"/>
        <property type="gene ID" value="ENSLOCG00000012742.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692478"/>
    <dbReference type="CTD" id="27152"/>
    <dbReference type="eggNOG" id="ENOG502QQJQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001301"/>
    <dbReference type="InParanoid" id="W5N4X4"/>
    <dbReference type="OMA" id="CAGKSIS"/>
    <dbReference type="OrthoDB" id="10038586at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012742">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043988">
        <property type="entry name" value="CCZ1/INTU_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039151">
        <property type="entry name" value="INTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21082">
        <property type="entry name" value="PROTEIN INTURNED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21082:SF4">
        <property type="entry name" value="PROTEIN INTURNED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19032">
        <property type="entry name" value="Intu_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="214"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="674"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="727"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="945" mass="104682" checksum="07A6EEFFA2A3CB18" modified="2014-04-16" version="1">MFNVGIDGRTSRPCSKNLEDFDSVRSVLLYSDLEPEWLDDVQKNGELFYLELSEGEEEVALAQSGAAQGPAANHVRFSDKEAEIITDGKRRKEGAKLEPKLKRLAKILRRKRLSRRWTDDDKGPSGCQNASQSSQPASILKNQSSQRPGVLVQQQLKDVCVYVNPKRLGNLLSTEGSRLLESLLGIVYHPSWARKVVGKGEEPEAACAEERLAVHGLVPGSPAIKCGQILIGDVLVAVNDVDVTSENIERVLSCIPGPTQVKLTFETASFDAEGTDSEHLNRKPSQPVSNLVKLLWGEDAVELQMSIGNIPHIVMYLSLKLDSESPKEEQEILYQYPVSEASRQLKGVRGIFLTLCDMLEGVTGGQIVSSTLLLGKHMVHVGYWKEGDNLLVIGLPAERVPLLHLKNVVGGLVRTLKVMYSSLDSAFCEADNMPRLDHFFCLFFQQLIQLPKPNGSPSSQPLDISSNLFLNSLPAVRWLTLPQEIKVEIDTVLSDFESSDFGDMSEDYYDMRRLYVILGSCLFYKGYLIGNHLPKEDLLDIALYCHHYCLLPLATEQRIGQLVIWREVFPQRQSQPSAEPAAPGYSEPDGRCFLLIVGLRHFMQCVLLEAGGCASAALGNPGPDCIYVDQVKATLLQLEGLDRSIEERLAAPPMPCLSCADWFLPSGRERLDSITSSPVFGKLPGSSKATSPTGRRVLFGEPALRTRRPSPQRSLSDSGSEGPLDNLSFNPGPSPHSTPDSTRKLGRRDSQGSGGSDGSAGSGSLFRITKKKHPNPFYLGTLKKSLNERETEEMYNTLKLTSGAENTLFHYVCMETVQGIFISPTHREVAQLSGSIHPQLIRNFHRCCLSIRAVFQRSLLSEDRKAADELQNVRGFGSVKEHGVLFQCKPENWTDQRKPPPSMTYWVIGRMLLEPTPQEFYVCFHDSVAEVPVEMAFRLSFGLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5C2</accession>
    <name>W5N5C2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase I subunit E</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5C2"/>
    <dbReference type="FunCoup" id="W5N5C2">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015831"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015861.1">
        <property type="protein sequence ID" value="ENSLOCP00000015831.1"/>
        <property type="gene ID" value="ENSLOCG00000012859.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018004"/>
    <dbReference type="HOGENOM" id="CLU_034953_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5C2"/>
    <dbReference type="OMA" id="DVYPFDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012859">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001188">
        <property type="term" value="P:RNA polymerase I preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="418" mass="47779" checksum="BEB2AF61827BC410" modified="2014-04-16" version="1">PSTEMAASYVWKHCNEEREERPPAAIVQFSNGKLRNADLVDFTLYRNTDLSNPRKKNRRILAAETDRLCYVGNNYGAEALKCNTLCKYYVGVLDKKTRTMNVHNVELFSMQPVIPGETVTEVDALEHKNKSYREKVDSLIEAFGTNKQKRALSSRRLNQVGSEMLHSAVAKAAENVIQKKGLQALERDVAQTEAQQEMSLLPCHADADKLEDVYLFDDIISPAEYEALEVPAQALKELSSEDVLKMREDGSPQVLIQEMQCLPKDEESREHMARCLWYLKQLIKLSSQRRINLKFSLEEGCPRIVVNRFLKTFTMETFSDGRINNTIPSSMKTKIAAYALALLLHIGDFQVDLTLLHRDLQLTENKMLDVARSMRLKIVKKSVLASGDVMEDHKIGVLELPLIMYNQRVERKKRKKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6Z3</accession>
    <name>W5N6Z3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">aromatase</fullName>
            <ecNumber evidence="11">1.14.14.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cytochrome P-450AROM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Estrogen synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of aromatic C18 estrogens from C19 androgens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>androst-4-ene-3,17-dione + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = estrone + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38195"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>testosterone + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 17beta-estradiol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38191"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.14" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01026099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6Z3"/>
    <dbReference type="SMR" id="W5N6Z3"/>
    <dbReference type="FunCoup" id="W5N6Z3">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016432.1">
        <property type="protein sequence ID" value="ENSLOCP00000016402.1"/>
        <property type="gene ID" value="ENSLOCG00000013305.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00840000129915"/>
    <dbReference type="HOGENOM" id="CLU_041874_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6Z3"/>
    <dbReference type="OMA" id="TMRFIPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013305">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019099">
        <property type="term" value="P:female germ-line sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008585">
        <property type="term" value="P:female gonad development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019100">
        <property type="term" value="P:male germ-line sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001541">
        <property type="term" value="P:ovarian follicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904076">
        <property type="term" value="P:regulation of estrogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20616">
        <property type="entry name" value="CYP19A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000032">
        <property type="entry name" value="Cytochrome P450 aromatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF43">
        <property type="entry name" value="AROMATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 16 17" id="KW-0349">Heme</keyword>
    <keyword evidence="7 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="517" mass="58836" checksum="3496B43B84DECF9F" modified="2014-04-16" version="1">STSFSYINWNMKQVPLDNVMVKMQNPVSHNITDVTPEMMSGATAILLPLLGLLVVALNCRAKQSIPGPSFCFGIGPIASYARFLWLGIGTASNYFNEKYGDIARVWINGEETLILSGSSAVYHVLKHGQYSSRFGSKKGLQCIGMYGKGIIFNSNVSLWKTVRTYFAKALTGPSLQQTVAICAVSTNKHLDCLHTVSNPEGQVNVLHFLRCTIMDINNKLFLRVPLDEEDLVHKTQRYFDSWQALLIKPDIFFKFKWLYEKHSKAAQELPEAIERLVEKKRKLLLETEKLDEIDFTSELIFAENHGDLSSGNVNQCVLEMLIAAPDTMSVSLYFMLMLLKQHPDVEQKLLEEIDTLIGDKDLQNGDLQNLKVLENFIYECLRFHPVVDFTMRRALEDDVIEGYRVKRGTNIILNMGRMHRQEFFPKPNEFSLENFENNVPSRFFQPFGSGPRSCVGKHIAMVMMKAILVTLLKRYTVSPQNGCSLSSIKHTNNLSQQPVEDRNTLDMIFIPRQKSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7I1</accession>
    <name>W5N7I1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox B13a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638382.1">
        <property type="nucleotide sequence ID" value="XM_006638319.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7I1"/>
    <dbReference type="FunCoup" id="W5N7I1">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016590"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016621.1">
        <property type="protein sequence ID" value="ENSLOCP00000016590.1"/>
        <property type="gene ID" value="ENSLOCG00000013457.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684356"/>
    <dbReference type="KEGG" id="loc:102684356"/>
    <dbReference type="CTD" id="559921"/>
    <dbReference type="eggNOG" id="KOG0487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159029"/>
    <dbReference type="HOGENOM" id="CLU_059940_1_0_1"/>
    <dbReference type="InParanoid" id="W5N7I1"/>
    <dbReference type="OMA" id="HPPDGCA"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013457">
        <property type="expression patterns" value="Expressed in larva and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000130">
        <property type="entry name" value="Homeobox protein Hox-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12284">
        <property type="entry name" value="HoxA13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="238"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="240"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="307" mass="33810" checksum="53C209A38ED68F15" modified="2014-04-16" version="1">MTTSLVLNPRWVDTVMFVYENNLDELNKNMEGLVGAGNFAANQCRNLMAHSALGGHPSTLVHGSGYSAVDVSGSGSGEPGKQCAPCPAVPQGTSTAPPLPYGYFGNGYYSCRMGRGSLKSCTQPGALSSYSAEKYMDTPVPSEEYPSRAKEFAFYHGYASPYQPMASYLDVSVVQTISGPGETRHETLLPVDSYQPWALPNGWGSQVYCPKDQSQAGHLWKSALADVVAHQQDGSSFRRGRKKRIPYTKVQLKELEKEYAANKFITKDKRRKISAATNLSERQITIWFQNRRVKEKKFVAKVKSSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N814</accession>
    <name>W5N814_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hairy-related 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01030446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N814"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016773"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016803.1">
        <property type="protein sequence ID" value="ENSLOCP00000016773.1"/>
        <property type="gene ID" value="ENSLOCG00000013605.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165786"/>
    <dbReference type="HOGENOM" id="CLU_068550_6_0_1"/>
    <dbReference type="InParanoid" id="W5N814"/>
    <dbReference type="OMA" id="PPLWRHQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013605">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11462">
        <property type="entry name" value="bHLH-O_HES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000063">
        <property type="entry name" value="transcription factor HES-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032644">
        <property type="entry name" value="HES-7_bHLH-O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="24737" checksum="C584626B702FC5DD" modified="2014-04-16" version="1">LAPIVGPVVTGQKEIPARQLVPKPLLEKRRRERINQSLETLRVLLLENTKNEKLRNPKVEKAEILESAVHFLKEEEGEEAQTGAGPRLLPRPPPPPLWRHQQTYREGMRSCLLRVGHFIAAKSRALEDSEGPGPAAWLSTPRSPGRAPWPGHLQPAVPHPAPKRPKLGVCTPPHGSHRLPESTGFCPGWQGSAGSRETDRTGAAPEGPKEVAMPGQEVWRPWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8E6</accession>
    <name>W5N8E6_LEPOC</name>
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        <submittedName>
            <fullName evidence="5">Cilia and flagella associated protein 100</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CFAP100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8E6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016905"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016935.1">
        <property type="protein sequence ID" value="ENSLOCP00000016905.1"/>
        <property type="gene ID" value="ENSLOCG00000013709.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSDI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153110"/>
    <dbReference type="InParanoid" id="W5N8E6"/>
    <dbReference type="OMA" id="SSENWIL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013709">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="157"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="391"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="608" mass="70736" checksum="A8AB72FC35AB8CD3" modified="2014-04-16" version="1">MSSPSLAPSSPGSPETVASGKLSRAHTTLLRSGTASLNPSEKSGVETKSVVTVPEDDNQVNKNPFKLPADNDIFLLRDKERERMKLVREQQKKLKIHEKTTYAGRMNTKRVETRRAMKEGEEMTMDTSTYKIISNSPINRQVLLKYRNIERESINEFISKKREMFLLQYSLAVKRDEIRKLDEVAAAEESKLEKAEQFLEDDAAMFDEFLKENDKNSVEAIKIAEQEAKVKLEKVAEKKRLSAKIVSIKSDISKYEDILKEYMTYKEFLLKLSPKEWQQKYEEKHRKNKQARAELKNKENESEQACSCHSACVREKKKRMDSRSSNVTQQGGREVTSVRDSRMSSRHSLKPSSSSRKLSSRSQIDEDNSSDDDDEEPELYFTEPQQLLDILTELEEQNLSLIQNSRETEEALEEFKHTMVNTQKKMDQETEILKQQIDILKQTIEREKERAAELELKARLFSFGQYKSEDQHTYCMLYNDVVTRVSLCSFFGNSKLNRANIQSVSAFPSSSYDIQYMYSRTAISQQYLSVLSINTTNFILLREEKIKMQKQHQEERLRKALERAQVDIKKPAGKRLMFRSQPPARKVKENKDQDITDKEKEEQLYFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8F7</accession>
    <name>W5N8F7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkey-1h4.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8F7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016916"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016946.1">
        <property type="protein sequence ID" value="ENSLOCP00000016916.1"/>
        <property type="gene ID" value="ENSLOCG00000013720.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163916"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_15_1"/>
    <dbReference type="InParanoid" id="W5N8F7"/>
    <dbReference type="OMA" id="YILANCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013720">
        <property type="expression patterns" value="Expressed in testis and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002109">
        <property type="entry name" value="Si:dkey-1h4.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF48">
        <property type="entry name" value="MULTIFUNCTIONAL ROCO FAMILY SIGNALING REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="253" mass="28364" checksum="7B171C88CAC2095D" modified="2014-04-16" version="1">DSETMDAEKLIMDSLYKQSEVLNLSHRQLFVPPPSLSHLTNLKRLFLNNNRLLLPPQEILHLQRLELLVLDDNQLTVLPSGIGALHHLNSLSVSHNPLSVLPVEIGDLTELRELWAVGCSLISLPASVGKLKKLQKIGLHNNHLSTLPPEFVHLTDLQWLNLACNELCDLPEGFGKLQALVFLNLEGNKFEHVPETLSDMVSLLALCMKSNCITSLPDDRILGFTRLTKLDLRENPLTLRPAHWKGLDFILLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N950</accession>
    <name>W5N950_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Glucagon receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635572.1">
        <property type="nucleotide sequence ID" value="XM_006635509.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N950"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017159"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017190.1">
        <property type="protein sequence ID" value="ENSLOCP00000017159.1"/>
        <property type="gene ID" value="ENSLOCG00000013910.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692630"/>
    <dbReference type="KEGG" id="loc:102692630"/>
    <dbReference type="CTD" id="562973"/>
    <dbReference type="eggNOG" id="KOG4564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165036"/>
    <dbReference type="HOGENOM" id="CLU_002753_4_0_1"/>
    <dbReference type="InParanoid" id="W5N950"/>
    <dbReference type="OMA" id="PPCWNPL"/>
    <dbReference type="OrthoDB" id="5967113at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013910">
        <property type="expression patterns" value="Expressed in liver and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004967">
        <property type="term" value="F:glucagon receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <sequence length="507" mass="58223" checksum="C03204D31876CE1C" modified="2014-04-16" version="1">MSQAFLLLTLLTFSTSIQVRSELTLTDIVKNWVSYKDECNHNISSEPPATGLVCNRTFDRYACWPDGLPNTVVNVSCPWYLPWHDRVRHGVVYRVCGLDGQWVLGPDKHPWKNTTECEVDDTDKQDYWKTLSMFWVMYTVGHSLSLGALVLALGILVAFRKLHCMRNYIHMNLFASFILRAVSILVKDALLHPPSSNFTLPKDNEVEKWLNSTDETPVGCRAALVMMQYSIMANYNWLLVEGIYLHNLLVITVFTERNYFKIYLCIAWGAPLLFVLSWVRLKYLYENIQCWERNLNMAVWWVIRSPIQLAILINFFIFIRIIQILVSKLRAHQMRYTDYKFRLAKSTLTLIPLLGIHEVVFAFVTDEHAQGSLRLVKLFFELFSSSFQGLLVAILYCFVNKEVQSELLKRWKRWKLGRDIEEEYRHTYSQTPQVRNGSTAAGAAPAPGEEHKLVGSYQNGTGHSKHSLQPPAPLEGAACTSTLKESSSLGDKAHCYEFPLESAESNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA24</accession>
    <name>W5NA24_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
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            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
        </alternativeName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000014192.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:serine-tRNA ligase activity"/>
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        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
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    </dbReference>
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        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
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    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="250"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
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        <location>
            <begin position="415"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="450"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="506" mass="57399" checksum="BA6CB5524FABC27C" modified="2014-04-16" version="1">MAAPMGVFLRGGFVSLKKPLDPRRFWGSAGGVGRRCRHTGRSSLYEHVREGYSDKPVLDMRFVCEHTDRVITEVESRKGDLRGEDVRGIVSVWQELQAVGEDIARLEQEKREVSEKVRALVEQQDKAVLPSVNGTAKWCPEKTLGHNIQRFGIPSHYELHFTNTYFNSPKKALERSGNRSWLSHCRHFHGLYNGPSIFIESPRSNRQQTIPIDSTEQDTSPSESTEQEAHTHRHTLENMRNEDPFPLFQGFVPMVVPDMLKGAVFEGCGMQPHAHQSQVYSLDPSRFPDLNLAGTGEVGVAGYFMDHAVNWRDLPVRTVCSSTCYRAETDTGREAWGLYRVHHFTKVEMFGVTADETGQESSELLLEFLSLQKEIFSDLELHYRVLDMPTQELGPPAYRKFDIEAWMPGRGSFGEISSASNCTDYQSRRLHIMYENEEGSLRYAHTVNATACAVPRTIIAILETHQTRDGTVRVPKVLQPYLGSEVIEKPRCTPLRYIGPNQPHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAB8</accession>
    <name>W5NAB8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRPM8 channel-associated factor homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCAF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAB8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017609.1">
        <property type="protein sequence ID" value="ENSLOCP00000017577.1"/>
        <property type="gene ID" value="ENSLOCG00000014278.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQUS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017365"/>
    <dbReference type="HOGENOM" id="CLU_1092246_0_0_1"/>
    <dbReference type="InParanoid" id="W5NAB8"/>
    <dbReference type="OMA" id="MWIVADE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014278">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.30:FF:000001">
        <property type="entry name" value="TRPM8 channel-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.80:FF:000001">
        <property type="entry name" value="TRPM8 channel-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.80">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.30">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042279">
        <property type="entry name" value="Pep_M60_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031161">
        <property type="entry name" value="Peptidase_M60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051244">
        <property type="entry name" value="TCAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730">
        <property type="entry name" value="EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730:SF5">
        <property type="entry name" value="SI:CH211-210B2.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13402">
        <property type="entry name" value="Peptidase_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01276">
        <property type="entry name" value="M60-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51723">
        <property type="entry name" value="PEPTIDASE_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M60" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="280" mass="32231" checksum="60DC99EE95974710" modified="2014-04-16" version="1">MAPFFRLGESSVSQWRSSARHCPAPWAELEADSVILTVPSDSVCSLDDPTPLLSLWNRITKGVAKLAATAPRFPRQERIVADVQISAGWMHSGYPIMIHLKSVKEIADLQHMKEAGMWGPIHEMGHNQQRELWDIRPHTTEATCNLWSVYIHEEVLGIPRHKAHEELRPHTRKNRIKDYVRKGATLEHWHVWTALETYLQLQEGFGWKPYMQLFSDYQEMSGTGLDNKGKMNLWAERFSLQVKRNLAPFFRAWGWPIEEPLAAKLAALPAWEENPMSSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCK6</accession>
    <name>W5NCK6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="15">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">Clathrin-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Synaptic vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006230">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCK6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018365"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018397.1">
        <property type="protein sequence ID" value="ENSLOCP00000018365.1"/>
        <property type="gene ID" value="ENSLOCG00000014912.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155068"/>
    <dbReference type="HOGENOM" id="CLU_022916_3_0_1"/>
    <dbReference type="InParanoid" id="W5NCK6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014912">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF5">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B, BRAIN ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="17">
        <location>
            <begin position="51"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="203"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="18">
        <location>
            <begin position="404"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="509" mass="56693" checksum="EA003832DA9BF267" modified="2014-04-16" version="1">MALKAIRGMVNGAMNELSSTVSGTKAAGAREHVQAVTRDYISQPRLTYKTVSGVNGPLVILDQVKFPRYAEIVHLTLPDGTRRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPTVMAEDYLDIMGMVTNCAHLTYPDNMASKKSSCGISSLSTKCNCVILIAAWPCHLQRSRSQCDKSEILKRNKDVMDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMRAVVGEEALTADDLLYLEFLQKFEKNFISQGAYENRSVYETLDIGWQLMRIFPKEMLKRIPQSTLAEFYPRDSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCN8</accession>
    <name>W5NCN8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018397"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018429.1">
        <property type="protein sequence ID" value="ENSLOCP00000018397.1"/>
        <property type="gene ID" value="ENSLOCG00000014943.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175676"/>
    <dbReference type="HOGENOM" id="CLU_1197243_0_0_1"/>
    <dbReference type="InParanoid" id="W5NCN8"/>
    <dbReference type="OMA" id="RSEGQHD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014943">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="255" mass="30374" checksum="36FA641C3DC5523A" modified="2025-10-08" version="2">MQLKGRTIRVDHVKNYRPPKDSEDIDDVTKRLREEGCAPRMPSPSSPESEEEEHTVTVKKQKKVKXEKKKKKKEKKEKKKVKEERRHARSCSAQTPPPALVMSARVKREEEDPGYEKYSHQEAPEDRPREEGRRTRPDRDHREDAGLHGDQAGGVGRERRDEGRAGGRERDGEPRRARDEEREDEAWRRGRHDDGTARERARDRDRERDRRDHRQWKSGGHSRDRSETADRERDRPDRGREHSRDAEHRRKKKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDL6</accession>
    <name>W5NDL6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ZIP4 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017025">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDL6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018725"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018757.1">
        <property type="protein sequence ID" value="ENSLOCP00000018725.1"/>
        <property type="gene ID" value="ENSLOCG00000015211.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4814">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006492"/>
    <dbReference type="InParanoid" id="W5NDL6"/>
    <dbReference type="OMA" id="NYETQMN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015211">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013940">
        <property type="entry name" value="Spo22/ZIP4/TEX11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38487:SF1">
        <property type="entry name" value="PROTEIN ZIP4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38487">
        <property type="entry name" value="TESTIS EXPRESSED 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08631">
        <property type="entry name" value="SPO22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="922" mass="103898" checksum="FEB2943590709A6E" modified="2014-04-16" version="1">REGKLTPETRDLTEELVQKQTPGSAERLIDRLFGEAARLDGELAGEVPQPQAEEHAIKLWNWAVTKHVGSAITEEQRAKVRHVACRLAYAYETKDLSEGAIRRQILMAIKTGRTWLDSRKPTLADTFFSLAVKSLEVLYSRLTARGDAKSDLNIPKTDVEKDLFRVLSYQAESAVAQGSNQEAVSCMQRCRDMLHRLPKESGYLSLMCYNFGVDTYKLKKFEESSFWLSQSYDIGKMDRKYSPGPAVQAKVLRLLATVYFEWDCQLYQEKALNAVTLANKENVHPAGLYLQVRILLKSGAPDEHLKVAVTELIDADVPLEVCLNTAKLLMAEDREALGFDFLKRVCKHHEASPEVGSALVLHIELLLQRGKELLGKQKIEDVITGHYTGRQLSPPTLDHFQLLLWDRASKNFESRNYSEALQWYNYSLSFYSAGQMDSNLAKLQRNRASCFLHLQQLDKKAKEALKEAERCEPDSIFTQFSVYKIAVLESNTDKAIEAVNAMGKQAENPVRNEDGLLVAENAASNLLCLAAQMALEKEQQDVAMKALECLCEHPRDSGQNFTALRCLVRLVLSKMGTTSEGKSNRDTNLDRLQSYLKMGTTALQKVSQANPGSNLSSEKTREAANWFRKIAWNAALQCENYPDRMRDFFVLSYRLSQFCPADKAILIGQKTCLLMAAAACLELGRTPESSEQAEKLTQALEHIDICWEVWKTLKASGDFSKDPTDTLLLLYEFEARAKLSDPNVETVLESVLQLPQIEAKTLEIIASLAMEPPAHFPSLCKKALRIALSLHKKSPSLDVSRCSQCLHSLIQLSLPTGLLEVEPRLLEEVWGYYEEALFIIGVAQGEFPEVEILWLLTRAWNTGILLYSLGQFPEAEKWCGLGMSFLRHLGSLQESYQTQMSGLYAEVLDRLDRVKRNVIDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDN8</accession>
    <name>W5NDN8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDN8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018747"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018779.1">
        <property type="protein sequence ID" value="ENSLOCP00000018747.1"/>
        <property type="gene ID" value="ENSLOCG00000015233.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3859">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158026"/>
    <dbReference type="InParanoid" id="W5NDN8"/>
    <dbReference type="OMA" id="NNGIHIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015233">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061640">
        <property type="term" value="P:cytoskeleton-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071907">
        <property type="term" value="P:determination of digestive tract left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003140">
        <property type="term" value="P:determination of left/right asymmetry in lateral mesoderm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071910">
        <property type="term" value="P:determination of liver left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035469">
        <property type="term" value="P:determination of pancreatic left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000036">
        <property type="entry name" value="septin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="39"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="321"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="437" mass="50378" checksum="C97920D859CD0CE1" modified="2014-04-16" version="1">MAATEIARQAGEGARSIPLSGHVGFDSMPDQLVNKSVNHGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPTQHNQPGVQLRSNTYELQESNVRLKLTMVNTVGFGDQINKEDSYKPIVEFIDTQFEAYLQEELKIKRTLHSYHDSRIHACLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKSDAISKSELTKFKIKITSELVSNGVQIYQFPTDDETVAEINSTMNGHLPFAVVGSTEEVKIGNKMVKARQYPWGTVQVENENHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFMGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQEEKKKLEDKKKVLDDEVNAFKQRKTATELLQSQGQPGGSQTLKREKERKNSSHYFLAHCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDR6</accession>
    <name>W5NDR6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203073.1">
        <property type="nucleotide sequence ID" value="XM_015347587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDR6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018775"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018807.1">
        <property type="protein sequence ID" value="ENSLOCP00000018775.1"/>
        <property type="gene ID" value="ENSLOCG00000015256.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683980"/>
    <dbReference type="KEGG" id="loc:102683980"/>
    <dbReference type="eggNOG" id="KOG0074">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155737"/>
    <dbReference type="HOGENOM" id="CLU_040729_12_0_1"/>
    <dbReference type="InParanoid" id="W5NDR6"/>
    <dbReference type="OMA" id="CKHISAR"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015256">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="20454" checksum="2D1C65D32B0BFBAE" modified="2014-04-16" version="1">MGLLSILRKLKSSPDQEVRILLLGLDNAGKTTLLKQLASEDVSHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWRNYFENTDVLIYVIDSADRKRFEETGQELAELLEEEKLSGVPVLVFANKQDLMTAAPAAEIAEGLNLHIIRDRIWQIQACSAVTSEGVQDGMMWVCKNIAPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEB1</accession>
    <name>W5NEB1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Kelch-like family member 13</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts with AURKB.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000018970"/>
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    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
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            <end position="200"/>
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            <begin position="1"/>
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            <begin position="1"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056604"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064290"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="694" mass="77980" checksum="1EC39DDAD9E8DA64" modified="2014-04-16" version="1">MRGRQYGHRRGAALGEGMDHPAHRGDLMSIGLHDRAPLSKGALNLYSDRGKDTEMNNSKEECSEDGKCILTRSLVEEDDPHMKVSLGSGDMGVSAHLQTSKTGNTRFFTSNTHSSVVLQGFDQLRIEGLLCDVTLLPGDGDEAFPVHRAMMASASDYFKAMFTGGMKEQDLMCIKLHGVNRIGLKKIIDFIYTAKLSLNMENLQDTLEAASFLQIIPVLDFCKVFLISGVSLENCVEVGRIANAYNLTEVDKYVNNFILKNFPSLLSTGEFVKLPFDRLAFVLSSNSLKHCSELDLFKAACRWLRYEENRMEYASKLMKNIRFPLMSPQELINHVQTVDFMRTDNICVNLLLEASNYQMMPFMQPVMQSERTAIRSDSTHLVTLGGVLRQQLVVSKELRLYDEKAHEWKALASMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRYDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRNAAGELATVECYNPRTNEWTYVAKMNEPHYGHAGTVYGGFMYISGGITHDTFQKELMCFDPDTDKWTQKAPMTTVRGLHCMCTVGDRLYVIGGNHFRGTSDYDDVLSCEYYSPALDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPEKDEWQKVFDLPESLGGIRACTLTVFPPEDNTGSPSRESPLSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEE0</accession>
    <name>W5NEE0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Regulator of G protein signaling 17</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218017.1">
        <property type="nucleotide sequence ID" value="XM_015362531.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218018.1">
        <property type="nucleotide sequence ID" value="XM_015362532.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069036054.1">
        <property type="nucleotide sequence ID" value="XM_069179953.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069036055.1">
        <property type="nucleotide sequence ID" value="XM_069179954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEE0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018999"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019031.1">
        <property type="protein sequence ID" value="ENSLOCP00000018999.1"/>
        <property type="gene ID" value="ENSLOCG00000015438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693042"/>
    <dbReference type="KEGG" id="loc:102693042"/>
    <dbReference type="CTD" id="26575"/>
    <dbReference type="eggNOG" id="KOG3589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155393"/>
    <dbReference type="HOGENOM" id="CLU_059863_0_2_1"/>
    <dbReference type="InParanoid" id="W5NEE0"/>
    <dbReference type="OMA" id="KRQQAHI"/>
    <dbReference type="OrthoDB" id="10266999at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015438">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Regulator of G-protein signaling 17"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGS"/>
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        <property type="entry name" value="RGS_sf"/>
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    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF196">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RGS" evidence="11">
        <location>
            <begin position="75"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="23689" checksum="8DF8CCBDADFBEDB3" modified="2014-04-16" version="1">MRKRQHPHSEGPPQAPDQQRPRTCCLCWCGCCRCPWNEDLMEGRERQTCTKMESIEAAEEQLEPTLEEILSWSRSFERMMYSPSGRNLFRQFLRSEYSEENLLFWLACEDLKHEKNTKAVDEKARIIYEDYVSILSPKEVSLDSRVREGINRNLLEPNCDMYNEAQLQIYTLMHRDSFPRFLNSAFYKSLVESARGSSAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFW0</accession>
    <name>W5NFW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protection of telomeres protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">POT1-like telomere end-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFW0"/>
    <dbReference type="FunCoup" id="W5NFW0">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019519"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019551.1">
        <property type="protein sequence ID" value="ENSLOCP00000019519.1"/>
        <property type="gene ID" value="ENSLOCG00000015853.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018285"/>
    <dbReference type="HOGENOM" id="CLU_019567_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFW0"/>
    <dbReference type="OMA" id="NHVHLAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015853">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04498">
        <property type="entry name" value="hPOT1_OB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20374">
        <property type="entry name" value="Pot1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000119">
        <property type="entry name" value="Protection of telomeres 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000138">
        <property type="entry name" value="Protection of telomeres 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048953">
        <property type="entry name" value="POT1_C_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21375">
        <property type="entry name" value="POT1_C_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="11">
        <location>
            <begin position="162"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="774" mass="87109" checksum="0B18DECEE90ED78F" modified="2014-04-16" version="1">MSVHILTGDAQIPSTLQRIYIKDLNHDSQCTDKYVKGTVIMKHNLIALTRGEDMLKAVIEEHAHQHAKLSETTSINVFSFGKLAKDFSDKINQGDTIVITGFRLGMSPTVNKDKRHLCQIELEERRATIYVCPAAFSGQSSSVDSSGTSEHSSNNPKPKYTYVPLNELKPGEVANVYGIVTFFKQPFQTKGTDYCLVLTITDESGVKLGCSIFNEKLEALPQIYKIGDIVRFHRIKSLLFNSKLSGMTSLGFSALTFDGRVGTPVCPRTSSKTYHFGEEDRRIVEKLRQWAATIYPNLEISLATVQPKSFFDLTCQLLAKAEISRACVLLKVWDGTKCPHRLLKILVEPSAIEGNMAAIQDLQDLIVEILVYDNHVEVAKKLKPGMYLRIYNLHAKLQASGTKEQTSNECHLGFHLHGGTSYGRGIKVLPEDSSDVQELKKALESANPEDTMDDLTLLNACCTSAIENSLNAELSPYQEDPLTVLTCHHTVKSDLLSNVKNSTPPQKFHIKAKLKSYQPDKLYQSVKLYCPKCKRVQEIPDDETIGSLFQEALNLPVLSSDSWYETFLWESSTDVGRKVAVHFVNVLGSEEHLLTLVQGATLEEVCRLSTKFESIIPIKFSEDQMTLLDLSAPFLIQGKKWSYGCKHCSCVKNIGLYLEQEMVWEPENTAEVLGVELLQYVFLMKFELDDGTASLDAILWEDAEKFFQMLPVEVMVNRAMQEKMQMTMATLCPPGKHFDELPWLEFCIGTYTTDENEDRSVYYQIFDTMVAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG01</accession>
    <name>W5NG01_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">24-hydroxycholesterol 7-alpha-hydroxylase</fullName>
            <ecNumber evidence="20">1.14.14.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Cytochrome P450 39A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Oxysterol 7-alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Steroid metabolism; cholesterol degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.26" evidence="20"/>
    <dbReference type="EMBL" id="AHAT01008375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG01"/>
    <dbReference type="FunCoup" id="W5NG01">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019560"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019592.1">
        <property type="protein sequence ID" value="ENSLOCP00000019560.1"/>
        <property type="gene ID" value="ENSLOCG00000015883.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682481"/>
    <dbReference type="KEGG" id="loc:102682481"/>
    <dbReference type="CTD" id="51302"/>
    <dbReference type="eggNOG" id="KOG0684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153141"/>
    <dbReference type="HOGENOM" id="CLU_558065_0_0_1"/>
    <dbReference type="InParanoid" id="W5NG01"/>
    <dbReference type="OMA" id="EYLLRNW"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015883">
        <property type="expression patterns" value="Expressed in zone of skin and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033782">
        <property type="term" value="F:24S-hydroxycholesterol 7-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20635">
        <property type="entry name" value="CYP39A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000060">
        <property type="entry name" value="Cytochrome P450 family 39 subfamily A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 24" id="KW-0349">Heme</keyword>
    <keyword evidence="13 24" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0492">Microsome</keyword>
    <keyword evidence="14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="24">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022848"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00049645"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056715"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066439"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071791"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00078877"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019560.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="52921" checksum="D31E666073692E98" modified="2014-04-16" version="1">MDFVILFLSVLIVLFSLYFLYLQRNQNYPPVIRGWIPWIGAAFEFSKAPLNFIAEARDKYGPVFTIIAAGKRLTFVTLHEDFQTFFTSKDVNFEQAVQAPVQHTASISRDSFLKFHPACNTLIKSRLILSNLPLLSSQLSEAFDEHLELLGTGSSGELCDLVRSAMYPAVMSNLLGKYNSPASHSDLQEFKTIFQTYDEGFEYGSQLPGIFFWKWAKSKQWLLSLLGKMILKAESSIPAEKSSKTLLQHLVTTITDRYLPNYGLLMLWASLANAIPISFWSLAFILSKPSVYQTVMEEIAVAFNGKDKRKTTVSQEELKQLPFLKQCVLEAIRLRAPGAITRMVVRPLQIQNYTIPAGDMLMLSPFWAHRNPEFFPEPEEYKPERWKKADLEKNVFLEGFVAFGGGKYQCPGRWYALMEIHMFVALALYKYEFTLLDPLAKPSSLHLVGTQQPVGSCRVQYQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGM5</accession>
    <name>W5NGM5_LEPOC</name>
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        <submittedName>
            <fullName evidence="5">GRAM domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000019784.1"/>
        <property type="gene ID" value="ENSLOCG00000016055.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Linkage group LG8"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of toll-like receptor 9 signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
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            <begin position="271"/>
            <end position="291"/>
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            <begin position="475"/>
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    </feature>
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            <begin position="109"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00568"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000019784.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="607" mass="69738" checksum="AB31C3B00A63BB4D" modified="2014-04-16" version="1">MKLCSDFRAGDAIHHVKVKQKISLLNMLKRLDKIRFRGHKRDEFLDLAESPNASDNECNDEIPVKPRASLKDTEDIRDPAGTGTLAMASAIQDFQRTESERLNEIKGHLEIALLEKHFLQEELRKLREETNVESLKQELEKERQKRIELEQKVNDVLKSRMEESQPPPKAQPAPVNGTDKRNETLCSRIQKWLYDKFGAYIEDFRFQPEENTIETEEPLSAKRLTENMRRLKRGAKPVTNFLRNLSALSNWHSVYTSAIAFIIYMNAAWHGWAIPMFLFLAILRLSLNYLIARGWRIQWSIVPEVSEPMEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADVLEKIKNLFMWVQPEITQKLYIGLWVAFISSCFLPYKLMGFIIGVYAGIKFFIIDFIFKRCPKLRDKYDTPYIVWTSLPTDPQLKERTNATVSRRLSGYEKVQPVASRSSLGTNAPCGINREEEGGRSHSTKKGAFHEIFNLADTERPLPACENGWRCCLINRDRKMPTDYIRNGVLYVTENYLCFESSKSGSSKKNKVIKLNDITDIQKYKVLSVLPGSGMGISIATPSTQKPLVFGAMIHRDEAFETIFTQYMKIMSSASNNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH42</accession>
    <name>W5NH42_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibronectin type III domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NH42">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019951"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019984.1">
        <property type="protein sequence ID" value="ENSLOCP00000019951.1"/>
        <property type="gene ID" value="ENSLOCG00000016185.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102688894"/>
    <dbReference type="eggNOG" id="KOG4221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063558"/>
    <dbReference type="InParanoid" id="W5NH42"/>
    <dbReference type="OMA" id="QIGFGTP"/>
    <dbReference type="OrthoDB" id="6129306at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016185">
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    </dbReference>
    <dbReference type="CDD" id="cd00063">
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049109">
        <property type="entry name" value="TARSH/FNDC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197:SF8">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197">
        <property type="entry name" value="TARSH-RELATED FIBRONECTIN DOMAIN-CONTAINING"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869314" evidence="2">
        <location>
            <begin position="26"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="123"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="227"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="329"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="1423"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="865"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="876"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="918"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="939"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="970"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1061"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1659" mass="183383" checksum="8745D1B1EBDBD0E1" modified="2014-04-16" version="1">MSPTTEWTLFVLFIPLFALSSTVKAERPLRPKNVKLMPDEKGLRVTWELPRDSQSRPVEHYNIGYGKSMKTLRYIKVGKNTQSQVLEDVEPGVLHFLKMTAENSDGVSQPVYKAETPPETKVPGRPLQVKGHTSEDSVSVQWKPPHSSGPDNPGRVRSYVLGVGEDNKRMHYTSLQDDQTTHETRRLASESVYVVSLQAQNALGQSSPVYRPVMTKKKASDSEELPEAKDITVRIISPQSVLVTWIDPAFEKQKDVVSASSRHYTVRYREKGESARWDYKESTQRRALIENLIADTMYEFSVRISKRERNGKWSVSVFQRTPESAPTSAPENFYVEPLKGKGTAATASWDPPQETNGRIREYILSYAPALKPFGTKSITYPGTTSSAIIDGLQPGDRYIFKIRAANRRGQGPQSKAFSVAMPTTSTIGSSSSQHSKVPDGRKKSTFRTSSNPASSSKVPWSHSTSKLPETGSRFTEEPEEPDSYTPVKASSPKPGTSHSSQNKDNETKDLIGQGPHSDSDENLEEEVDEHFEEKRVQHTKQNNAATTSDDSSSESSFKSDKISPGSVSKTRPQRPISPTNTGTNKMRRPATLPKAISPSKISKEKEDPPSTKSNAQESDFTRKIHTDSSYPNRREPDDHDKEKVPSFPSSKNFFSKKPLNSSVLFPKGGRVAVPANRQMGSGFINGRRPVGSQSRPSYTESRGSAQPETTPEQIGSSRILNSRLKPDLSSTVSQSTLSTGAQNPQSRSSHSQHLTKPQDNYQDNESTETETEEKKSASSLSYSTPSPDYDRGAQTSRLGTKEFVTSNDKHSSSPSTGTHTEAKILTSERTRSPSLSSNRFSVLRRHFPKQETKLNTSSGQRRVPSSSTRSQTSESNVAHTDSGASVTQETNEKPKVPISFPSEHASTGSSKRHPAVISESSNPVIPSDSSSSPPSQGHLDSDNPIHPKSKDEDDDYDYYGSKELYDTTDKLVSTTAEPQVTTSFLRKPQKPNPRFGKAHLPFVQSVPQRPAGGFNGKSRSPMFINRQSGSRDSESLLPSDYSKLPYVTSSHTSSERQHTASSSVPRTSSSQSSSASLPHLPPIREPNQGTRVRYPGRMDVATRVKTPSSALKPSYTQGKNGRPNLTNTNGKVVSTSDAKVKQGGRRLITGPEGVKWIVDFDKGFLMNQEGRILQDSKGRPRRVVLGEDGRTIFDDQGTPLVSPDGLALFGHGRDSRPVVNPKDKLLAVGGKPLVGLDPIKPTQKMTTTTTTTTTTTTTAATTTTTPEPTTIPTTTEMTTEESTTPFIFPTCPPGTYSQVDERGYPLLGEDGILDCYPEEFQFVQTTLSPTTTPTTPTTRESPEATTTETEEVYTEAMHFNNGPTSGFDAAGNPRFTAPYVNYIQKDPGAPCSLTEALEYLQVDVLENLFEKDRQSTHQPPKSKPHNITVVAMEGCHSFVILDWARPLKDDMVSGYMVHSASYDDVLNNRWSSSSANGTHLPVENLKPNSRYYFKVQAKNIFGLGPLSETFTYETESDDPLLIERPPGGEPIWIPFSFKYNPAQSTCKGSQYVKRTWYRKFVGVVLCNSLRYKIFMGEGLKETFYSIGDTYGHGEDHCQFVDSHMEGRTGPHYLSTYLPTVQGFFRAYRQEPVQFGLIGRRTSHPFVGWYECGVPIPGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI13</accession>
    <name>W5NI13_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRO1 domain-containing protein BROX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">BRO1 domain- and CAAX motif-containing protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BROX family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI13"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020306.1">
        <property type="protein sequence ID" value="ENSLOCP00000020272.1"/>
        <property type="gene ID" value="ENSLOCG00000016414.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698523"/>
    <dbReference type="KEGG" id="loc:102698523"/>
    <dbReference type="CTD" id="148362"/>
    <dbReference type="GeneTree" id="ENSGT00390000006681"/>
    <dbReference type="HOGENOM" id="CLU_056561_0_0_1"/>
    <dbReference type="OrthoDB" id="10266451at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016414">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd09243">
        <property type="entry name" value="BRO1_Brox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038898">
        <property type="entry name" value="BROX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032:SF13">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="5">
        <location>
            <begin position="85"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="46560" checksum="5A0CB9E104AC3E67" modified="2014-04-16" version="1">MTHWFHRNPLKATAPVSFSFYGVAASPAANKICNDLKAARARLLEMFTDPTCNPEIMKNATDSYFSLLQGFIVSLDGTTQENKMRFIQNFKWTDTLQGNTPSAQQDAVFELVSMTFNVGLWYSKFASRLAGKENITETEAKEVHKSLRIAAGIFKHLKDAHIPRLITPAEKGRDLELRVIDTYIIQCQAEAQEVTIARAIELKHNASLIGALAFETANFYKKADHTLNSLEPDYTSKWRKYFQLKQAFYMAYAYCYNGQMLLASDKCGEAVRSLREAEKYYTKAESLCKEYRQTKGPGSTAKPSEQLFFTKLGGLIKNTLEKCERENGFIYFQKVPQDPPDLELKASYGLVEPIAFEFPSVSPQCTPEVYATFDLTKNHKDDKAKPKQEEEVKPMKEPDLKTQKDTGCSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI72</accession>
    <name>W5NI72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Oxoeicosanoid receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI72"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020331"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020365.1">
        <property type="protein sequence ID" value="ENSLOCP00000020331.1"/>
        <property type="gene ID" value="ENSLOCG00000016459.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282516"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NI72"/>
    <dbReference type="OMA" id="ACKINLF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016459">
        <property type="expression patterns" value="Expressed in mesonephros"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051893">
        <property type="entry name" value="HCARs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46048:SF10">
        <property type="entry name" value="HYDROXYCARBOXYLIC ACID RECEPTOR 1-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46048">
        <property type="entry name" value="HYDROXYCARBOXYLIC ACID RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="35"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="335" mass="38560" checksum="816234596D503027" modified="2014-04-16" version="1">MAGNNSLFYCAEPHKNVGKVLVPILSLEFICGLVGNMLALWVFCFRIRSWKTYTVYMLNLVIADLLLIAGLPLRIINYHEQENWTFSEPTCRINLFMLAMNRTASICFLTTVSIDRYFKVVHPRHRFHVTTLAGAVKVACLLWVLTILMSIHLTLAKLVDINPQQEKNFTLCRSYSSYRNPTPGMIWHGVLFFMEFLVAFSLIIFCTFSILWQLRRIKMDSQAKVKHIQKLVILIMIMFAICFMPSIVAAVVALLVKSLYRDDCDKFNIFGRVFHGSLAFTYMNSVLDPVLYCFSSPSFRDNVKLTLQSICIWKKGSEVSSKHLSFSDEQWCTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIZ3</accession>
    <name>W5NIZ3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6</fullName>
            <ecNumber evidence="26">2.3.2.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">BIR repeat-containing ubiquitin-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Baculoviral IAP repeat-containing protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Ubiquitin-conjugating BIR domain enzyme apollon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the BIRC6 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.24" evidence="26"/>
    <dbReference type="EMBL" id="AHAT01001734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020602"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020637.1">
        <property type="protein sequence ID" value="ENSLOCP00000020602.1"/>
        <property type="gene ID" value="ENSLOCG00000016644.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698367"/>
    <dbReference type="CTD" id="57448"/>
    <dbReference type="eggNOG" id="KOG0895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156126"/>
    <dbReference type="InParanoid" id="W5NIZ3"/>
    <dbReference type="OMA" id="TTWDEHV"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016644">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000014">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000001">
        <property type="entry name" value="baculoviral IAP repeat-containing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022103">
        <property type="entry name" value="BIRC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12356">
        <property type="entry name" value="BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="24" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="32" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UBC core" evidence="34">
        <location>
            <begin position="4554"/>
            <end position="4721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="425"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="941"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1012"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1204"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="2926"/>
            <end position="2954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="3556"/>
            <end position="3576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="3903"/>
            <end position="3937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="4243"/>
            <end position="4262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="4794"/>
            <end position="4838"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="1589"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="33">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="33">
        <location>
            <begin position="1014"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="3560"/>
            <end position="3569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="33">
        <location>
            <begin position="4252"/>
            <end position="4262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="33">
        <location>
            <begin position="4808"/>
            <end position="4838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00039076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00060909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00069601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00075349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00079718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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        <source>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="4838" mass="526548" checksum="C3D70BB0A7BF81C4" modified="2014-04-16" version="1">[encoded data, 4838 characters]</sequence>
</entry>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <location>
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            <end position="37"/>
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            <begin position="49"/>
            <end position="70"/>
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            <end position="98"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020766.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="159" mass="16854" checksum="7990119493A14BA2" modified="2014-04-16" version="1">CAGVQMYLLCAGVQIYLLCAGVQMCLPCAGVQIYPLCAGVQMYLPCGGVQLYLLCAGVQIYPLCAGVQMYPLCAGLQMYLLCAGVQIYPLCAGVKIYLLCAGVQIYLLCAGVQMYPLCAGVKIYLLCAGVQMYPLCAGVQMYPLCAGVQIYPLCAGVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJK5</accession>
    <name>W5NJK5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Holostei</taxon>
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    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
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                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <subcellularLocation>
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        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF230">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="62" mass="7367" checksum="C316889ED9D1D970" modified="2014-04-16" version="1">MFEQVPMVEMDGMKLVQSKAIMNYISAKYNLYGKDLKERVMIDMYAEGLRDLMEMIMKHPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJY7</accession>
    <name>W5NJY7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LYR motif-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in efficient integration of the N-module into mitochondrial respiratory chain complex I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJY7"/>
    <dbReference type="FunCoup" id="W5NJY7">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000020982.1">
        <property type="protein sequence ID" value="ENSLOCP00000020946.1"/>
        <property type="gene ID" value="ENSLOCG00000016944.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S8DG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165360"/>
    <dbReference type="HOGENOM" id="CLU_151409_1_1_1"/>
    <dbReference type="InParanoid" id="W5NJY7"/>
    <dbReference type="OMA" id="YMRDWAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016944">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20262">
        <property type="entry name" value="Complex1_LYR_LYRM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045293">
        <property type="entry name" value="Complex1_LYR_LYRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF0">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="87" mass="10157" checksum="DC74A40A931174C4" modified="2014-04-16" version="1">MATSRLPPTALTLKQFIQRQKVLGLYRSMLRTIRRVPDAADRQFLQSWAREEFRRNRGATDQDAIRMMITQGNMHLQDLQKTLQLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLS7</accession>
    <name>W5NLS7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206775.1">
        <property type="nucleotide sequence ID" value="XM_015351289.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206776.1">
        <property type="nucleotide sequence ID" value="XM_015351290.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLS7"/>
    <dbReference type="FunCoup" id="W5NLS7">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021586"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021623.1">
        <property type="protein sequence ID" value="ENSLOCP00000021586.1"/>
        <property type="gene ID" value="ENSLOCG00000017481.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688307"/>
    <dbReference type="KEGG" id="loc:102688307"/>
    <dbReference type="CTD" id="404726"/>
    <dbReference type="eggNOG" id="ENOG502QUKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="HOGENOM" id="CLU_037388_0_0_1"/>
    <dbReference type="InParanoid" id="W5NLS7"/>
    <dbReference type="OMA" id="YLGFTKC"/>
    <dbReference type="OrthoDB" id="9930281at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017481">
        <property type="expression patterns" value="Expressed in embryo and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010644">
        <property type="term" value="P:cell communication by electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031444">
        <property type="term" value="P:slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
        <property type="entry name" value="Connexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF49">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118220">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="164"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="357" mass="40610" checksum="64E2FE6EB55F573B" modified="2014-04-16" version="1">MGDWSLLGKLLESAQEHSTVVGKVWLTVLFIFRILVLGAAAEKVWGDEQSGFTCDTKQPGCQSVCYDKTFPISHIRFWVLQIIFVSTPTLIYLGHILHLVRMEEKQKQKEKERITQAANQGDKQPLLGETKAKKPSVRDAQGRIQMQGVILRTYVFNIIFKTLFEVGFIVAQYFLYGFELKPLYTCDRLPCPNIVNCYISRPTEKTIFIVFMLAVACISLLLNLVEMYHLGFTKCRQGVSSKSEIASEMGSKDPNDTVVPFVPNYNYFPKQHSPTGPYQSGSRYSLSPLPEPDTAYHPYNSKAAYKQNRDNLAVERNSKPDESDLKVKKGTPVEKQRRPSRSSKHSNNKTRLDDLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLV7</accession>
    <name>W5NLV7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HEXIM P-TEFb complex subunit 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HEXIM family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLV7"/>
    <dbReference type="FunCoup" id="W5NLV7">
        <property type="interactions" value="1272"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021616"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021653.1">
        <property type="protein sequence ID" value="ENSLOCP00000021616.1"/>
        <property type="gene ID" value="ENSLOCG00000017511.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682190"/>
    <dbReference type="KEGG" id="loc:102682190"/>
    <dbReference type="CTD" id="10614"/>
    <dbReference type="eggNOG" id="ENOG502QQP8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002808"/>
    <dbReference type="HOGENOM" id="CLU_066028_0_0_1"/>
    <dbReference type="InParanoid" id="W5NLV7"/>
    <dbReference type="OMA" id="ALNPGCP"/>
    <dbReference type="OrthoDB" id="10058500at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017511">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024872">
        <property type="entry name" value="HEXIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469">
        <property type="entry name" value="HEXAMETHYLENE BISACETAMIDE INDUCIBLE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469:SF8">
        <property type="entry name" value="HEXIM P-TEFB COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15313">
        <property type="entry name" value="HEXIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02094">
        <property type="entry name" value="HEXIMFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="336" mass="36138" checksum="0F9F47903F92B35B" modified="2014-04-16" version="1">MSEPADPDSQRGPQSPGAPAGGSPEPPGQLAACEGGGAQAGARGRCGAGGPAQNGAAEEPGQARGSPEACPGEGGPAAQGRGGGALNPGCPGGGLPAIPEARLGKKKHRRRPSKKKRRWKPYFKLSWEEKKELDERETARASRVRAEMFAKGLPVAPYNTTQFLMDEHDLGEPDLNTELCGRRLGAAGRPEDTASEEDFPEPEDEEEGSGGGSDGMGRPGDGGGEFLQRDFSETYERYHVESLQNMTKQELVREYLELEKCMSRLEDENNVLRLRAAKGLDVPAESPAPEPAPDPRLQELQELQSELERLRAQNSELRLQNERWSRERGPGASPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLZ3</accession>
    <name>W5NLZ3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLZ3"/>
    <dbReference type="FunCoup" id="W5NLZ3">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021652"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021689.1">
        <property type="protein sequence ID" value="ENSLOCP00000021652.1"/>
        <property type="gene ID" value="ENSLOCG00000017547.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159475"/>
    <dbReference type="HOGENOM" id="CLU_040215_2_1_1"/>
    <dbReference type="InParanoid" id="W5NLZ3"/>
    <dbReference type="OMA" id="YRIIQAE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017547">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2440:FF:000001">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017434">
        <property type="entry name" value="Pase-1_reg-su_3B/C/D_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF13">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038207">
        <property type="entry name" value="PP1_GT_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 4" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="5">
        <location>
            <begin position="139"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="34882" checksum="9E6CAEF79FEFC118" modified="2014-04-16" version="1">FFSPPSSPSVLGFFPHKSAIPLELAMPLYLTRDDFRYKRIPKLGKPLRSCVHRTSILKVKPQDFSNHSEEKSKAKKQVSFADHKGFALTMVKIFSEFDDHIDIPLNIQELIDTVVNMSMEEDHLILDFAQPSSDYLAFRQRLEVDCVCLENCMLKEKSLVGTVKVKNLSFEKSVKMRVTFDTWKSFRDVECQYVKDTYTGSDKDTFSFEINLPSKIPPHERIEFAVLYECCGQTFWDSNQGQNYKVVRSVLKSSSQNGDKGGFQSSSAIEDLGIHCDLYGSPRCAHGIFPEWPSYAGYEKIGPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV60</accession>
    <name>W5LV60_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosomal protein L18</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="STRING" id="7918.ENSLOCP00000000017"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000017.1">
        <property type="protein sequence ID" value="ENSLOCP00000000017.1"/>
        <property type="gene ID" value="ENSLOCG00000000017.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012976"/>
    <dbReference type="InParanoid" id="W5LV60"/>
    <dbReference type="OMA" id="APLGQHT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000017">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="86" mass="9589" checksum="9FF8FC4F8584E501" modified="2014-04-16" version="1">XRVTDGARQRILKTGGQVMTFDQLALAAPKGEGTVLLSGPRKARKVYRHFGKATGTPHSHTKPYVRSKGRKFERARGRRASRGYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVA1</accession>
    <name>W5LVA1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVA1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000058"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000058.1">
        <property type="protein sequence ID" value="ENSLOCP00000000058.1"/>
        <property type="gene ID" value="ENSLOCG00000000049.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158061"/>
    <dbReference type="HOGENOM" id="CLU_082306_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVA1"/>
    <dbReference type="OMA" id="YPVEVMT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000049">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000001">
        <property type="entry name" value="Integrin alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF3">
        <property type="entry name" value="INTEGRIN ALPHA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0401">Integrin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="5">
        <location>
            <begin position="11"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="6">
        <location>
            <begin position="140"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="269" mass="30127" checksum="EAB25124291667C9" modified="2014-04-16" version="1">MSCGLLVCSDKKEVYLGDDNSLTLTFNARNEGEGGAYEAELYVIPPPEADYSGIVRNNESLSQLTCSYEMLNQTRVVICDLGNPMKSGTSLWGGLRFTVPNLDDSRRAVHFDFQIRSKNENNSRSDVVSYKLDVAVLSDVILQGVSRPDKVIFPPANWKATKRPVTEQDVGPLVHHVYELVNHGPSLISHTLLELHCPMKIQNHQLMYPVEVMTQGPLNCTTNKTINPQQLKVGRNSSTLYNNDDNNNNNNCLHLYSAFLDTPLKALYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW16</accession>
    <name>W5LW16_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW16"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000323.1">
        <property type="protein sequence ID" value="ENSLOCP00000000323.1"/>
        <property type="gene ID" value="ENSLOCG00000000297.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_988793_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW16"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="34079" checksum="56EABA59C79DC6A2" modified="2014-04-16" version="1">RPITVHMFNGNVPESIVSIFLKRFVDLQGEGKTVVDEENVWTAKWRYMARFRTSLMTPGGVLHPPATFNIGPNRGYIIYPGQPKTCRRCGQEGHLVVDCKTEICRRCGRIGHVATTCRHALVGVLSLIFKKEPLFARELRSMIVHMYNPHVPEPDIVTFLRRYVDVQGGGQKVVDEERIWTFKRRYLVRLRQCPTAPGGVLHPPANFTIGLNRGYLIYSGQPQTCRRCGQEGHLAAECSTEVCRWCGRTGHVAVVCTYQQLCNLCGESGHLYRVCPRKTRSFASMVSGKPAQPRVPKEQQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW37</accession>
    <name>W5LW37_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW37"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000344"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000344.1">
        <property type="protein sequence ID" value="ENSLOCP00000000344.1"/>
        <property type="gene ID" value="ENSLOCG00000000318.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074797"/>
    <dbReference type="HOGENOM" id="CLU_1474704_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW37"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000318">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000015">
        <property type="entry name" value="Neurofibromin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF142">
        <property type="entry name" value="NEUROFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="183" mass="20608" checksum="DD19100E49C0F36F" modified="2014-04-16" version="1">MNLLNDCSEVDDDGQQTAGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWP8</accession>
    <name>W5LWP8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWP8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000555"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000556.1">
        <property type="protein sequence ID" value="ENSLOCP00000000555.1"/>
        <property type="gene ID" value="ENSLOCG00000000505.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155806"/>
    <dbReference type="HOGENOM" id="CLU_1849685_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWP8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000505">
        <property type="expression patterns" value="Expressed in testis and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF4">
        <property type="entry name" value="ANKYRIN REPEAT AND SAM DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="139" mass="15290" checksum="3DA8FF8652F366E8" modified="2014-04-16" version="1">PCSCCFLFLFSQNLTYEIILTLGQAFEVAYQLALQAQRTKPHGVTSATGSEVIETKSTRPVPKPRGSVRKSAGAPLLESRCCYCHTCTTHRPSYLPLRSVSPGAQMEPLELETDSQSQGSATWLVDQKTDSKRTISTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXI1</accession>
    <name>W5LXI1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Growth arrest and DNA-damage-inducible, beta b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GADD45 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639842.1">
        <property type="nucleotide sequence ID" value="XM_006639779.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXI1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000842.1">
        <property type="protein sequence ID" value="ENSLOCP00000000838.1"/>
        <property type="gene ID" value="ENSLOCG00000000752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685769"/>
    <dbReference type="KEGG" id="loc:102685769"/>
    <dbReference type="GeneTree" id="ENSGT00950000182964"/>
    <dbReference type="HOGENOM" id="CLU_118164_0_0_1"/>
    <dbReference type="OrthoDB" id="5976967at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000752">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000012">
        <property type="entry name" value="growth arrest and DNA damage-inducible protein GADD45 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF5">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="160" mass="17582" checksum="C0234678917DADAD" modified="2014-04-16" version="1">MTLEEVVGCSIADQKMETVAQALEELLVTAQRQDSLTVGVYESAKLMNIDPDSVVLCILATDEEDEDDIALQIHFTLIQAFCCDNDINILRVSGVQRLAQVLGEAGADCNANEPRDLHCLLVTNPHTDSGKSQALKAVGSYCEESRCQNQWVPFLALQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXK7</accession>
    <name>W5LXK7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Deoxyribonuclease-2-alpha</fullName>
            <ecNumber evidence="4">3.1.22.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Acid DNase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Deoxyribonuclease II alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Lysosomal DNase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the clearance of nucleic acids generated through apoptosis, hence preventing autoinflammation. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver and bone marrow, where it degrades nuclear DNA expelled from erythroid precursor cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.22.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="W5LXK7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000864"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000868.1">
        <property type="protein sequence ID" value="ENSLOCP00000000864.1"/>
        <property type="gene ID" value="ENSLOCG00000000779.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2548859_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXK7"/>
    <dbReference type="OMA" id="QTWAHEG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000779">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF9">
        <property type="entry name" value="DEOXYRIBONUCLEASE-2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867270" description="Deoxyribonuclease-2-alpha" evidence="19">
        <location>
            <begin position="17"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="83" mass="8905" checksum="F7E71323DA15602C" modified="2014-04-16" version="1">FSLVQFLCLSAGGALAAVSCYNDQGSPVDWFYLYKLPQLSHHAPGSGLLYLLLQQGNDSWVKGASLVNKSDGALGRTVGQLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXR4</accession>
    <name>W5LXR4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Kininogen-1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXR4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000921"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000925.1">
        <property type="protein sequence ID" value="ENSLOCP00000000921.1"/>
        <property type="gene ID" value="ENSLOCG00000000828.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695287"/>
    <dbReference type="KEGG" id="loc:102695287"/>
    <dbReference type="CTD" id="3827"/>
    <dbReference type="eggNOG" id="ENOG502RYAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182930"/>
    <dbReference type="HOGENOM" id="CLU_548528_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXR4"/>
    <dbReference type="OMA" id="DDWHART"/>
    <dbReference type="OrthoDB" id="9937817at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000828">
        <property type="expression patterns" value="Expressed in liver and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030195">
        <property type="term" value="P:negative regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000002">
        <property type="entry name" value="Kininogen 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027358">
        <property type="entry name" value="Kininogen-type_cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050735">
        <property type="entry name" value="Kininogen_Fetuin_HRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814">
        <property type="entry name" value="FETUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814:SF12">
        <property type="entry name" value="KININOGEN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51647">
        <property type="entry name" value="CYSTATIN_KININOGEN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="8" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="2" id="KW-0840">Vasodilator</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865251" evidence="12">
        <location>
            <begin position="24"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin kininogen-type" evidence="13">
        <location>
            <begin position="30"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin kininogen-type" evidence="13">
        <location>
            <begin position="155"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="306"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="508" mass="53997" checksum="07C12F224F4C4C8F" modified="2014-04-16" version="1">MLNIKLLAIFSVSLLICLKGARSEKIQAFCDDKEVELAVDHAILEYNKNLTGGNQLALFQISEAWKEVSDTGTEISLLFLVKESDCPVGSDKVWKQCDYVANSQLRAPWPCTATVVINHQEKSQDVTSVKCVASVEPNIVPEFYLCAGCPHEIDSNSDSVTEILQLAMEKLNAQDNSSHHFIVSAVTKAVSQVVSGILYNIDFEIRLSNCSKSEVVNITSDCHPDEDRNVFAQCNSSVVVAPWRQTTDPTVTCGPQVHVPNTFARRPPGWSPFRTAAVAIDGARPVAQPLPGRLMQQDVPMIRLDRGLHSGRGHSPGHVQGPGQSHSRGQGHGQGHGQGHGQGHGQGQGKGHGQGHGQGHGQGHGQGQGQGHGQGQGQGHQEEFPPFLLQDPDVSSEESSEATGKPPVLATAESPVLVVESATQGSGPGPVIGQDLTEAGGVPLGSAPIPTNPTAPTTGPAISLGFPLRETPSFHCPSKPWKQEPPQTNPTSPPLNPPSSFDDLDLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ28</accession>
    <name>W5LZ28_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hdc homolog, cell cycle regulator</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_015194551.1">
        <property type="nucleotide sequence ID" value="XM_015339065.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ28"/>
    <dbReference type="FunCoup" id="W5LZ28">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001385"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001390.1">
        <property type="protein sequence ID" value="ENSLOCP00000001385.1"/>
        <property type="gene ID" value="ENSLOCG00000001223.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697198"/>
    <dbReference type="CTD" id="51696"/>
    <dbReference type="eggNOG" id="KOG3816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044928"/>
    <dbReference type="HOGENOM" id="CLU_027672_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZ28"/>
    <dbReference type="OMA" id="NHDIQRR"/>
    <dbReference type="OrthoDB" id="10012848at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001223">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026066">
        <property type="entry name" value="Headcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031947">
        <property type="entry name" value="Headcase_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054537">
        <property type="entry name" value="HECA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425">
        <property type="entry name" value="HEADCASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425:SF3">
        <property type="entry name" value="HEADCASE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16002">
        <property type="entry name" value="Headcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15353">
        <property type="entry name" value="HECA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Headcase N-terminal" evidence="2">
        <location>
            <begin position="83"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Headcase middle" evidence="3">
        <location>
            <begin position="340"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="543" mass="60235" checksum="8B55D305B26EE852" modified="2014-04-16" version="1">MPNQKNNKGKRNKRTNSSGDEQENGASAAATAAQSVAATLLGATAALANENSSGDNFCYVVVDNSTSGSELLHLGSKTIEAPCATPLVCSLGRSIDLEKDDYQRVVCNSEVCPYSNWMHLQCFYEWESSILVQFNCIGRARSWNEKQCRQNMWTKKGYDLAFRFCSCRCGQGHLKKDTDWYQVKRMQDDRKKKVLPEKSLGKLASGGGGESPEDPKKGRHSAGNKLAHKAASHELPRRQSIDRQNSQEKGPAGSFCGNSLGPRSPCDSPGQSPPSGFSFFSPSSFTGPRGSRHLGEFLKNAVHMESHRKHLSTGGATSRNAHGEHAGSLSLPRLASADTPVQFLRRLDLSELLAHIPRHKLNTYHVRMEDDAHAGQGEDLRKFILAALSASHRNVVNCALCHRVLPVFEQFPLVDGTLFLSPSRHDEIEYDVPCHLQGRLMHLYAICVDCLEGVHKIICIKCKSRWDGSWHQLGTMYTYDILAASPCCQARLNCKHCGKPVIDVRVGMLYFSEYSNVQQCPHCGNLDYHFVKPFSSYKVLEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ39</accession>
    <name>W5LZ39_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 25">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="7 25">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 25">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="7 25"/>
    <dbReference type="EMBL" id="AHAT01025131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ39"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001396"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001401.1">
        <property type="protein sequence ID" value="ENSLOCP00000001396.1"/>
        <property type="gene ID" value="ENSLOCG00000001205.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047027"/>
    <dbReference type="HOGENOM" id="CLU_021018_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZ39"/>
    <dbReference type="OMA" id="KFADDEW"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001205">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04608">
        <property type="entry name" value="CBS_pair_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000014">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046353">
        <property type="entry name" value="CBS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 25" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 24 25" id="KW-0129">CBS domain</keyword>
    <keyword evidence="18 25" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 25" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CBS" evidence="27">
        <location>
            <begin position="414"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="565" mass="62109" checksum="EA6A9368C701FFD2" modified="2014-04-16" version="1">MPSVPVKAENDSSAGSCPVSAQSHQGAVNGDDSTVEMKWIRPDTPSKCTWRLGAPPTESPHLHLELTKPPGIMPNILMNIGNTPLVRINKIAKAYGIKCEVLAKCEFFNAGGSVKDRISLRMVEDAEKAGILKPGDTIIEPTSGNTGRIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTSARFDSPESHVGVSWRLKNEIPNSHILDQYRNPSNPLAHYDSTAEEILQQCDGKVDMLVAGAGTGGTITGIARKLKEKCPGCKIIGVDPEGSILSQPDELNKTDKTTYEVEGIGYDFIPTVLDREVVDDWYKSNDEESFNMSRLLIKEEGLLCGGSSGSAMAAAMKAAKELEEGQRCVVILPDSIRNYMSKFLSDKWMFQKGFLTEDSMVTKPWWWNMKIQELNLSAPLTVLPTVTCQKTIEILKEKGFDQAPVVNESGLILGMVTLGNMLSSVLAGKVQLFDPVNKVLYKQFKQIHLNDNLGKLSRILEIDHFALVVHEQIQYLSDGSPCLKQMVFGVVTAIDLLNFVTAKEKKQEVSLGKRKKVVRFSNFLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZU5</accession>
    <name>W5LZU5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZU5"/>
    <dbReference type="FunCoup" id="W5LZU5">
        <property type="interactions" value="1529"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001652"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001657.1">
        <property type="protein sequence ID" value="ENSLOCP00000001652.1"/>
        <property type="gene ID" value="ENSLOCG00000001443.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695474"/>
    <dbReference type="KEGG" id="loc:102695474"/>
    <dbReference type="CTD" id="51143"/>
    <dbReference type="eggNOG" id="KOG3905">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008295"/>
    <dbReference type="InParanoid" id="W5LZU5"/>
    <dbReference type="OMA" id="KKATNCD"/>
    <dbReference type="OrthoDB" id="27603at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001443">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036372">
        <property type="term" value="P:opsin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009794">
        <property type="term" value="P:regulation of mitotic cell cycle, embryonic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097474">
        <property type="term" value="P:retinal cone cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF2">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 LIGHT INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="54902" checksum="098566C7EDCF7658" modified="2014-04-16" version="1">MAAVARSASTSSNPAVNTAGSETHNGGEEDGQNLWSSILSEVSTHSRSKLPSGKNVLVMGEDGAGKTTLIAKLQGIEEYMKGRGLEYLYFSVHDEDIDDLTRCNAWILDGDLYHKGLQKFAVSTDSLSDTVVLFVVDMSRPWTSLDSLQKWASVIREYIDKLKIPPEVMKEMEQKLVKQFQEYADPGADLTVSPQRRNPSVPDGDDESIVLPLGENTLTHNLGVPVIVVCTKCDAISSLEKEHDYKDEHFDFIQLHIRRFCLQYGAALIYTSMKENKNLDLLYKYIVHRLYGFPFNSPAQIVEKDAVFIPAGWDNEKKIAILHENFQSLKAEDNFEDIIVKPPVRKFVHEKEIAAEDDQVFLIKLQSLLAKQPPVAAGRPVDATNRGPGGSPRTPVRSGSSNVASVMPVPANAKKIDPNMKAGTTSEGVLANFFNSLLSKKTGSPGAGGGSPAGGGSCVPGSAKKSVQKLGLTDVQAELDRISSKPDTEASSPATPTSPAEDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0A5</accession>
    <name>W5M0A5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0A5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001812"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001817.1">
        <property type="protein sequence ID" value="ENSLOCP00000001812.1"/>
        <property type="gene ID" value="ENSLOCG00000001581.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158585"/>
    <dbReference type="HOGENOM" id="CLU_749170_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0A5"/>
    <dbReference type="OMA" id="REPYKIE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001581">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF258">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="3">
        <location>
            <begin position="147"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="348" mass="39012" checksum="AC3091DCA09C8109" modified="2014-04-16" version="1">TPGEQERFPERVRLAMFISGQNHTVHLFKTRDLLSEGYTEHYYERGSGLSAVSVRPQDHCCYTGWVEGARDSSVSLCSCQGFSGSLRLGEHSYTVTPETDGGPRHTLTGTRLKNMIYFQHVCSELVLNSNIMPELILISLQGSDQKWGIELFLVADHEEFQRQHGDKRRTQWKLITLAHHLKEIYSQVGIDVWLVGTEVWTEGDKVTVDYSTTETLKNFLHWREEDLLPRVPHDNAQLISGRRFKGKVLGEGLLGTMCSSSQSGGLAQSPGVSAWELAEIVAHEMGHNLEMHHDNEPGRSCQCPSWSGKCLLSAFTGISRLGQSTVFSNCSISSLKRFLEKDKASCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0Y2</accession>
    <name>W5M0Y2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01038855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0Y2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002039"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002044.1">
        <property type="protein sequence ID" value="ENSLOCP00000002039.1"/>
        <property type="gene ID" value="ENSLOCG00000001762.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159936"/>
    <dbReference type="HOGENOM" id="CLU_002360_4_1_1"/>
    <dbReference type="InParanoid" id="W5M0Y2"/>
    <dbReference type="OMA" id="CMEIVTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001762">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF15">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 16" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="840"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="943"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="975"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="37"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1013" mass="111556" checksum="6989CB7DD0C7FA9C" modified="2014-04-16" version="1">MGKERGRDHTPNQNGRDKKKKQKEKDLDELKKEVSLDDHRITLDELGRRYGVDLSRGFTTAQAVEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYSIQAAMEDEPANDNLYLGVVLAAVVIITGCFSYYQEAKSSRIMDSFKNMVPQQALVIRDGEKLQINAEEVVLGDLVEIKGGDRIPADLRIISSHGCKVDNSSLTGESEPQTRSPEYTHDNPLETRNIAFFSTNCVEEISFFLYRGAVCVCGGVGGGRGFLRLLNSGLEVGRTPISMEIEHFIHIITGVAVFLGVSFFALSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIYEADTTEDQSGSTFDKSSPTWTALAHISGLCNRATFKPGQENVLILKRDTAGDASESALLKCIELSLCNNRKQLTMKQNRNALTRTSSPSLLQLSIHEFQDNPKGHILVMKGAPERILDRCSTILIQGQEVPLDEDMKDAFQNAYMELGGLGERVLGFCHYHLSPDKFPRGFRFDTEDVNFPTEDLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVRVTTTASAAAARTRLAEPCLKNPQTVADSSSPNLRPAAGPSLLTCWAEGSPNASIDKTWPIESLSGLRVDFLAGSPNPLSMKTPGSADLSACRRRGSNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIALLTQLTLLLTHLRTVEPDGSLFSCAESPGSYWCSGPANLLVTPLSPQTYEQRKIVEFTCHTAFFSSIVVVQWADLIICKTRRNSVFQQGMKNRILIFGLFEETALAAFLSYCPGMDIALRMYPLKITWWFCAFPYSLLIFIYDEIRKLILRRNPGGWVEKETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M147</accession>
    <name>W5M147_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Interacts with target proteins during translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation and facilitate correct glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SEC61B, SEC61A1 and the SEC61 complex. Interacts with CANX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RAMP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M147"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002104"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002109.1">
        <property type="protein sequence ID" value="ENSLOCP00000002104.1"/>
        <property type="gene ID" value="ENSLOCG00000001822.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161729"/>
    <dbReference type="HOGENOM" id="CLU_1547045_0_0_1"/>
    <dbReference type="InParanoid" id="W5M147"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001822">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601">
        <property type="entry name" value="STRESS ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN SERP1/RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601:SF14">
        <property type="entry name" value="STRESS-ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="69"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="173" mass="19138" checksum="B2A56A5AB47090D4" modified="2014-04-16" version="1">MIFMADYIAGQAARRKDCAGRGGVGRRREGPSVCRRRQGPLFITAWCGATHRSWRAGSPLLISALHTSSLLLRPPPPPPPQQQQQSTARPGDRTQTHGGEKQQQVNKMVAKQRIRMANEKHSKNITQRGNVAKSTRNAQDDKVSVGPWLLALFIFVVCGSAIFQIIQSIRMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M182</accession>
    <name>W5M182_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="14">PEAMT</shortName>
            <shortName evidence="14">PEMT</shortName>
            <ecNumber evidence="14">2.1.1.17</ecNumber>
            <ecNumber evidence="14">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phospholipid methyltransferase</fullName>
            <shortName evidence="14">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PEMT</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Mitochondrion membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="14">Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="14"/>
    <dbReference type="EC" id="2.1.1.71" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01031987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M182"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002139"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002144.1">
        <property type="protein sequence ID" value="ENSLOCP00000002139.1"/>
        <property type="gene ID" value="ENSLOCG00000001840.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007041"/>
    <dbReference type="HOGENOM" id="CLU_086119_1_0_1"/>
    <dbReference type="InParanoid" id="W5M182"/>
    <dbReference type="OMA" id="PTFWNIA"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001840">
        <property type="expression patterns" value="Expressed in intestine and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005444">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="14">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="14">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="14">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="14">
        <location>
            <begin position="154"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="14">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="237" mass="26483" checksum="3776643042A2943A" modified="2025-10-08" version="2">MSGGDSPIYLEQNKKKKTSLLDCCGGLNNLDYNKIDMSVMAEVLHYVDVTDAQFSVAVLAIIFNPFFWNVVARWEHRTKGLTRVFGCPYAACYCLALLIILLNVYRSHSFTMAMNSQPKAELMERSEMFYAGAILVFIGSVFVLSSFYALGFTGTFLGDYFGILQEEKVNGFPFNVMENPMYWGSTAIYLGLALMNASPAGVVLTSVVAAAYKIAIMFEGPFTEEIYRQRISSEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1I4</accession>
    <name>W5M1I4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Ras p21 protein activator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">p120GAP</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Interacts with tyrosine-phosphorylated EPHB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626615.1">
        <property type="nucleotide sequence ID" value="XM_006626552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1I4"/>
    <dbReference type="FunCoup" id="W5M1I4">
        <property type="interactions" value="1557"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002242"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002247.1">
        <property type="protein sequence ID" value="ENSLOCP00000002242.1"/>
        <property type="gene ID" value="ENSLOCG00000001929.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688757"/>
    <dbReference type="KEGG" id="loc:102688757"/>
    <dbReference type="eggNOG" id="KOG3508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155846"/>
    <dbReference type="HOGENOM" id="CLU_010968_1_0_1"/>
    <dbReference type="InParanoid" id="W5M1I4"/>
    <dbReference type="OMA" id="KMGSYLV"/>
    <dbReference type="OrthoDB" id="1562946at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001929">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001568">
        <property type="term" value="P:blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08400">
        <property type="entry name" value="C2_Ras_p21A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13260">
        <property type="entry name" value="PH_RASA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05391">
        <property type="entry name" value="RasGAP_p120GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10354">
        <property type="entry name" value="SH2_Cterm_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10353">
        <property type="entry name" value="SH2_Nterm_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11788">
        <property type="entry name" value="SH3_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000007">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000090">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000050">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000052">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000033">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000046">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035842">
        <property type="entry name" value="RasGAP_C_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035841">
        <property type="entry name" value="RasGAP_N_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035652">
        <property type="entry name" value="RasGAP_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF146">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="265"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="337"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="460"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="565"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="21">
        <location>
            <begin position="736"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="41"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1035" mass="116523" checksum="CDC0DF0DE6420DBF" modified="2014-04-16" version="1">MMAAEVGSEEAGSVSAGTGVSAAGGPEAHFAFFAQPRLRVSESAPGAAPALLHHRPPDGPSEVVLCPVAGAGSGTGAGQPSPSGGSSSAGAGAAGPPTPEAQGIRMSPTSGGPDGPAAAFPPLPPPPPPPPVCGGLGTVDENDMLDGAEYEEEEVAFPLTAPPTNQWYHGKLDRTIAEERLRQARTPGSYLIRESDRRPGSFVLSFLSKTSVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSYVSCLLKGEKLQSPVAPPEPVEDRRRVRAKLPYTKVPDTDEISFLKGDMFIVHNELDDGWMWVTNVRTDEQGLIVNDLVEEVGREEDPHEGKVWYHGKINKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLFFRTTENIQRFKICPTPNNQFMMGGRYYNSIDDIIDHYRKEQIVEGYYLKDPVSVQHQEQVLSDLVDREEIYNTIRRKTKDAFYKNIVKKGYLLIHKGKGKRWKNLYFILEGNDAQLIYFESEKRATKPKGLIDLSVCSVYGVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAQDWMKCLQTFCSNLRKPTQPSSHKRLRQVSSLVLYVEEAHKLPVKHFTNPYCNIYLNSVQVAKTHPREGQNPVWTEEFIFDDLSSEINRFEISLSNKTKKSKDPDILFMRCQLSKLQKGQMIDEWFPLSSNVPLKGIEPGSLRVRARYSVEKIMPEEEYEDFKQLVLQKEFHAIYALAHVCGQDRTLLASILLRIFRHEKAEAPLLRTLNDREISMEDEATTLFRATTLASTLMEQYMKATATPFVHHALKDTILKIMESKQSCELNPSKLEKNEDVSINLIHLLNILSELVEKIFMAAEILPPTLRFIYGCLQKSVQQKWPKNTTMRTRVVSGFVFLRLICPAILNPRMFNIITDPPSSTAGRTLTLIAKSVQNLANLVEFGAKEPYMEGVNPFIKHNKHRMIMFLDELGNVPDLPDTTEHFRTDMSRDLAALHEICVTHSDELRTLSNERGAQQHVLKKLLAIAELLLQKQKQYSMSNSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1T6</accession>
    <name>W5M1T6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">LSM14A mRNA processing body assembly factor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LSM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1T6"/>
    <dbReference type="FunCoup" id="W5M1T6">
        <property type="interactions" value="1095"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002344"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002349.1">
        <property type="protein sequence ID" value="ENSLOCP00000002344.1"/>
        <property type="gene ID" value="ENSLOCG00000002022.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154415"/>
    <dbReference type="HOGENOM" id="CLU_019221_0_1_1"/>
    <dbReference type="InParanoid" id="W5M1T6"/>
    <dbReference type="OMA" id="WYPPPGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002022">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000006">
        <property type="entry name" value="Protein LSM14 homolog A isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
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    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
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    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF2">
        <property type="entry name" value="PROTEIN LSM14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="8">
        <location>
            <begin position="285"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="9">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="10">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="5">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="6">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010415"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="481" mass="52221" checksum="02DA2A36CFF0E684" modified="2014-04-16" version="1">MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIPPRDEVFEYIIFRGSDIKDLTVCEPPKPQCSLPQDPAIVQSSLGSSPAASFQSVASYGFSRGPVSAYSQFSPSPLVGQQFGAVGVGIGRSLTSSGTDTSTPAPLSQSNTIGSAFSPETSLLKTQLHRGRSSPQLDPLRKSPTLEQAVQTAPAAAPVPVGRRSPAARPTPAGTQKPGESQEQRRSTDAQKLPRPDAEQVRNENRDPAKRQSAPPPARRGRGGHRGRGRFGVRRDGPMKFEKDFDFESANAQFNKEEIDREFQNKLKLKDDKPDKPEKAVNGEDKGDSGVETQNSEGNADEEDPLGPNCYYDKSKSFFDNISCDDSRERRQTWAEERRMNAETFGIPLRHGRGRGGFRGRGAMGFRGGRGRGGGRGAFGTPRGGGGGSSGFRGGFRGGRGGREFSEFEYRRTTKLLHSTSGRWIQTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M400</accession>
    <name>W5M400_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M400"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000003115.1">
        <property type="protein sequence ID" value="ENSLOCP00000003108.1"/>
        <property type="gene ID" value="ENSLOCG00000002610.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166845"/>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_1"/>
    <dbReference type="InParanoid" id="W5M400"/>
    <dbReference type="OMA" id="HERIQDC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002610">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF146">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="102"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="661" mass="73818" checksum="03BE3D666D5C7370" modified="2014-04-16" version="1">MVVPGSHHTEEQVFPPPLELQNDAHVPDFQSYLQLYKKSVDEPEVFWKEVAQDFFWKSPPKGQFLQYNFDVTRGSIYVKFMEGAKTNMCYNVLDRNVKEKNLGEKVAYFWEGNSPDRQRSITYRQLLKEVCRCANVLKSMETGVGKGDRVAVYLPMIPELVCVMLACARLGAVHSVVFAGFSSESLCERILDSQCLFLVTADGVFRGEKLINLKQIADEALEKCKEKGNFTIKTCIVVRHVGDKMKNGIVSNKLQTHWTAGVDVWWDEAMASAGEECEPEWVEAEDPLFILYTSGSTGKPKGVLHSVAGYLLYTALSFKCVFDHQPGDVYWCTADIGWITGHSYITYGPLANGATSVLFEGIPVYPHVGRFWEIIEKYGVSKFYTAPTAIRLLMKYGQEPLKQFNLDSLKILGTVGEPINPEAWLWFYNVVGQQRCPVLDTFWQTETGGHVLTPLPSAIPLKPGSATLPFFGVQPAILNEHGEELEGEAEGYLVRAKTPYPGYQCFIFLIEQRNAAVLPTQSQHGKGLSGPGCRRDKDGYYWITGRIDDMLNVSGKDSKNKLLDSLMNNQAQGSQLVVAHRTTHCSMDYTRLNLVINTKKGIYCTGDAIDGLPVFPVTAGKILRRVLRQIARGEKDLGDLSTLADPRVVEVLSSQRSEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4A7</accession>
    <name>W5M4A7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coactosin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with 5-lipoxygenase (ALOX5/5LO) in a calcium-independent manner. Binds to F-actin with a stoichiometry of 1:2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the actin-binding proteins ADF family. Coactosin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641350.1">
        <property type="nucleotide sequence ID" value="XM_006641287.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4A7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003222.1">
        <property type="protein sequence ID" value="ENSLOCP00000003215.1"/>
        <property type="gene ID" value="ENSLOCG00000002734.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697266"/>
    <dbReference type="KEGG" id="loc:102697266"/>
    <dbReference type="CTD" id="23406"/>
    <dbReference type="GeneTree" id="ENSGT00390000012498"/>
    <dbReference type="HOGENOM" id="CLU_129657_1_0_1"/>
    <dbReference type="OrthoDB" id="20822at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002734">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11282">
        <property type="entry name" value="ADF_coactosin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000018">
        <property type="entry name" value="Coactosin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF29">
        <property type="entry name" value="COACTOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="142" mass="16008" checksum="FDE552C369E0D0EF" modified="2014-04-16" version="1">MATKIDKEACREAYNLVRDDNTDVNWASFVYDGSTIVPGGQGTEYEEFKNQCTDDARLFGFVRITTGDAMSKRAKFTLITWIGENVSGLQRAKISTDKTLVKDIVQNFAKEFMVSDRRELEEAYIRAELKKAGGANYDAQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4R9</accession>
    <name>W5M4R9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">EI24 autophagy associated transmembrane protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EI24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4R9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003377"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003384.1">
        <property type="protein sequence ID" value="ENSLOCP00000003377.1"/>
        <property type="gene ID" value="ENSLOCG00000002857.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3966">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018633"/>
    <dbReference type="HOGENOM" id="CLU_031164_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4R9"/>
    <dbReference type="OMA" id="HMCLLYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002857">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389:SF0">
        <property type="entry name" value="ETOPOSIDE-INDUCED PROTEIN 2.4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389">
        <property type="entry name" value="P53 INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="354" mass="40100" checksum="EA6E2B5322F12F44" modified="2014-04-16" version="1">PLPSAVDDLGDEPVMADSVRTFLYGVATGIKDSILGIGTISKLDARIQQKREEQRRRRASGALAQRRAQSEERRQESDPKLASRILQCCAWNGGVFWLSLFLFYRVFIPLLQTLTARIIGDPSLHGDVWSWLEFILTSVFSALWVLPLFVLSKIVNAIWFQDIADLAFEVSGRKPQPFPSVSKIIADMLFNLLLQALFLIQGMIVSLFPIDVVGQMISLLHMSLLYSLYCFEYRWFNHGIEMHQRLSNIERNWPYYFGFGLPMALLTALPSSYIISGCLFSILFPLFIISANEAKSPVKPYHFQLRLFSLVVLISNKLFHKTMNLQSSLTAAAPCERLASPRASPARQRPLLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4Z8</accession>
    <name>W5M4Z8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Growth differentiation factor 6a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635621.1">
        <property type="nucleotide sequence ID" value="XM_006635558.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4Z8"/>
    <dbReference type="FunCoup" id="W5M4Z8">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003456"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003463.1">
        <property type="protein sequence ID" value="ENSLOCP00000003456.1"/>
        <property type="gene ID" value="ENSLOCG00000002936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687575"/>
    <dbReference type="KEGG" id="loc:102687575"/>
    <dbReference type="CTD" id="566470"/>
    <dbReference type="eggNOG" id="KOG3900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160140"/>
    <dbReference type="HOGENOM" id="CLU_020515_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4Z8"/>
    <dbReference type="OMA" id="KKSKYRC"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002936">
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="9 12" id="KW-0339">Growth factor</keyword>
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    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
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            <begin position="22"/>
            <end position="410"/>
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        <location>
            <begin position="287"/>
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            <begin position="288"/>
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    </evidence>
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    </evidence>
    <sequence length="410" mass="46934" checksum="215A13901806F67C" modified="2014-04-16" version="1">MDAFRAAVFSLLLICLWDVPALQSAALFSSPVPRNNKGTKILNDGRKAPKSLKEILSSSTLRSHHADALKDAIVPHDYMLSIYRTYSAAEKLGLNASFFRSSKTANTITSFVDRGQDDLSHSPLRRQKYLFDVSTLSDKEELVGAELRIFRKVPGDFQMSETGLYGIQLLSCRSGRLLDSRSLDLQDSQRPGWEVLDVWELFKNHRPPAHQNQVCFELKATFGTSEREMDLRHFGFERHHRRQQEKAILVVFTRSRKKENLFNEMKEKIKSSRKAGDPSGFRLKARRRRRTAFNNRHGKRHGKKSKSRCSKKPLHVNFKELGWDDWIIAPLDYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPNSTPPSCCVPTKLSPISILYIDSGNNVVYKQYEDMVVESCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5F2</accession>
    <name>W5M5F2_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Thymocyte selection associated family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the themis family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631438.1">
        <property type="nucleotide sequence ID" value="XM_006631375.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5M5F2">
        <property type="interactions" value="708"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000003610.1"/>
        <property type="gene ID" value="ENSLOCG00000003069.1"/>
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    <dbReference type="eggNOG" id="ENOG502QSJR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063770"/>
    <dbReference type="HOGENOM" id="CLU_022319_1_0_1"/>
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        <property type="component" value="Linkage group LG6"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
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    </dbReference>
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        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
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        <property type="entry name" value="THEMIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15215">
        <property type="entry name" value="CABIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15215:SF2">
        <property type="entry name" value="PROTEIN THEMIS2"/>
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        <property type="entry name" value="CABIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="3">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="CABIT" evidence="3">
        <location>
            <begin position="272"/>
            <end position="499"/>
        </location>
    </feature>
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        <location>
            <begin position="556"/>
            <end position="676"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="612"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="665"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00006414"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="687" mass="77297" checksum="FDE5AB64C558E608" modified="2014-04-16" version="1">MAGSSGEVLSLQEYIASVDASTLPRVLQVCSGVYFQGSVYELSGSEVCLSTGDLVKVIHTELLSVSCQDVKTQETFELPASHSGLFKLVQEQVSYSSIEEIVGLMPIGGENISPFTFATCHALALGKYTLHSGEMLTFLSEETCKGQPHARCLLQNQAGSPEVLIPFSCRGEFYQYVNNNQYSISQILASPQLRCRRFLPVKAMNWNGSSGGPLIFTPVHQVQAIMHLRKNVVKFPSNLEVDVTDVTEQSKDVTFILPLSLKEVSSQPDHAFPTVAEILEEPENQTFFKCHWMSALRKGQQLIVHGHQKSHMVLASSLTGKKAKQHFFISQSYAGRLRRRPREFATVYDLFVASSKLPGLRVNVSKHCESPEEDLPSLSVGEQLEVLFTKQMELNCDGEQQIIDVLVCNRFLDEDDEDEEEQVQEQYEQICLPMYLEGNFVEKLKDNKKYSLVDIYKSFCLPLDVKVAVRDPGLENDPLGGFSALKLEQTTVEPTVLASLPDRPDVCFELPSQWLNMSLYFTDNPLPWPKDQPPKLRRETVFEVTDNFYYEFHKLISSDMPPPPRPPKRKKSSKHSPASPFPPSSVQPKGKALPTLPLEDSLPPRMDKLTISPETQSDTSLQILDHTPPLLPRKPKQRNTKTQPNEYVMRPHKDKKKKVPASPSDSDHDYETVDDSMKAAQESILFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5L6</accession>
    <name>W5M5L6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 191</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000003674.1"/>
        <property type="gene ID" value="ENSLOCG00000003111.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    </dbReference>
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        <property type="entry name" value="CACF_protein"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="183"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="275"/>
            <end position="310"/>
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        <location>
            <begin position="464"/>
            <end position="561"/>
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        <location>
            <begin position="603"/>
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    </feature>
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            <begin position="396"/>
            <end position="459"/>
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            <begin position="564"/>
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        <location>
            <begin position="279"/>
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        <location>
            <begin position="475"/>
            <end position="494"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
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        <location>
            <begin position="667"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="700"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="921" mass="107358" checksum="076D9055E05A4237" modified="2014-04-16" version="1">MCLENRRILMNNVGHNPELFRWKRLTKSKTPVQKVQLKNDDIDHWMKRVEAASEFAVSEVFSLHKPVGNSKIRGHAMALESTEQLQDHDDAFSEAQTLLSEWVNSKLRLELETEDEDGLGHVCETDRSEPAPRVQDGPDFLRYDNFDDLYSHLEQEAESSAVHSFLQDLMEKEVVESGVMDDLRMDTERKRRSDPRVTMEVRHQQVKESRARREAEQERRRRDSALQREARAAARRLLQGEERRKRLEAQQQEELIQREVVRLRKDMAEQRSVAGLARQMERERQERKRSAGGTAHAEPIAGMGVPKSSPQPLVRQAAQMQLQHEQLEAERLRRLRETEARTHILNLRRLQKHFSAWYSVVLERRLQAGKAAALCDWRSQLRAWRAWQALVWARRAERTEEELRGHNRKCQRALESDRRRLLRHFLGEWRRWCRARRERRRLQAQQEETRRKMAALISAASSGELWAGRPAGQGATASSEQHQPAAQTESTQPSAKREETDLASPPPAPAVRDGGGRPASRPKHAWQVTKKHAALTAEDLSRNAQQPPPGGAAGGGGGGFEHRHAFQQRMLEQQRRQLREQQEMILELQENQRLLMLRQEAERAQASLPAPPAQKPSSGGTALGESRSGAAVQSQGENHSKEENNTLSVSRAPLRPSCSPHPAVTAMEERAKQRAVRRKEIEEMKRKREEERIAQLQAAEEERQRQEEAERQAAAERKREERRLQRQREQERLTRLEREQQLQARASDHYLCTLLTRRGLAPWKRLLEQARRNAQLAETHHGHSLLRRCFSSWLQTAGESLAEKEARAEQLYRRILLRRCLHHWLKCKDYCSVLEEKAGRLCRARLQRKTLLALLDYCTRERMARWDREQLAERHSRRRAVVSCFRAWRGFPHLLREEREREARRERLRRRVAEVLPDFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5V0</accession>
    <name>W5M5V0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uroplakin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024158">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5V0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003758"/>
    <dbReference type="TCDB" id="8.A.90.1.3">
        <property type="family name" value="the uroplakin 2/3 (upk2/3) family"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000003764.1">
        <property type="protein sequence ID" value="ENSLOCP00000003758.1"/>
        <property type="gene ID" value="ENSLOCG00000003180.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006115"/>
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    <dbReference type="OMA" id="SFMVPPC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009952">
        <property type="entry name" value="Uroplakin-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17573">
        <property type="entry name" value="UROPLAKIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17573:SF0">
        <property type="entry name" value="UROPLAKIN-2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07353">
        <property type="entry name" value="Uroplakin_II"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865689" evidence="2">
        <location>
            <begin position="27"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="188" mass="19690" checksum="CE7C3BDE42FDC1C1" modified="2014-04-16" version="1">PANPAGAAMGALLLAALGLLLAAADADFTVNVLGESDGLVTGQLSDSVILSLPPCSLAGNTVNMVYQQGGTQNKVNLTGIFKVPVCRFKRDLITVIANSGQFTVTRNIGYQVKNLSPATNYTFQYFVGSEKSNAVQAATRTVSNYQDIDDGLPARSGAMVVITVLLSVAMFILIVGLIVTLVLAHCRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6J9</accession>
    <name>W5M6J9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-associated domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Kip1 ubiquitination-promoting complex protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222311.1">
        <property type="nucleotide sequence ID" value="XM_015366825.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6J9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004007"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004015.1">
        <property type="protein sequence ID" value="ENSLOCP00000004007.1"/>
        <property type="gene ID" value="ENSLOCG00000003381.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684569"/>
    <dbReference type="CTD" id="10422"/>
    <dbReference type="eggNOG" id="ENOG502QQQ6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014658"/>
    <dbReference type="HOGENOM" id="CLU_035938_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6J9"/>
    <dbReference type="OMA" id="WLIQNDS"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003381">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd14303">
        <property type="entry name" value="UBA1_KPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14304">
        <property type="entry name" value="UBA2_KPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17066">
        <property type="entry name" value="Ubl_KPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000006">
        <property type="entry name" value="Ubiquitin-associated domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041926">
        <property type="entry name" value="UBA1_UBAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041927">
        <property type="entry name" value="UBA2_UBAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052476">
        <property type="entry name" value="UBAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057650">
        <property type="entry name" value="UBL_UBAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46738">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46738:SF1">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23326">
        <property type="entry name" value="UBL_UBAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="194"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="300"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="417" mass="47017" checksum="B0642CE7A2237CFF" modified="2014-04-16" version="1">MFVQEEKIFAGKVLKIHICTMEGAEWLEEVTEDTTIEKLKEKCLKHYVHGSLEDPKTVTHHKLIHAASERVLADTKTVVEENIKDKDVLLLIKKRPPPPPPKMTEVSTEEKKKQEQKAPDKDAILKATANLPVRHSDRTVAQHNIRDAVPFFGHKKFQTELRKILVSLIEVAQKLLALNPDAVELFKKANAMLDEDEEDRVDEMALQQLTEMGFPESRAVKALRLNHMSVTQAMEWLIEHVDDPTVDAPLPGQETAGAAAPPAGTSIATARAPSESNAEDSKQDELTEIFKRIRRKREFRPDSRAVIALMEMGFDEKEVIDALRVNNNQQDAACDWLLGDRKPSPEDLDKGIDTNSPLFQAILENPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6U4</accession>
    <name>W5M6U4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Sphingomyelin phosphodiesterase 3</fullName>
            <ecNumber evidence="6">3.1.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Neutral sphingomyelinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Neutral sphingomyelinase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N-(15Z-tetracosenoyl)sphing-4-enine-1-phosphocholine + H2O = N-(15Z-tetracosenoyl)-sphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45320"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74450"/>
            <dbReference type="ChEBI" id="CHEBI:74535"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:45321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-(hexadecanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-hexadecanoylsphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
            <dbReference type="ChEBI" id="CHEBI:78646"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:45645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N-(tetracosanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-tetracosanoyl-sphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72965"/>
            <dbReference type="ChEBI" id="CHEBI:83360"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:45325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="3.1.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:19254"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus membrane</location>
            <topology evidence="20">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01010562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6U4"/>
    <dbReference type="FunCoup" id="W5M6U4">
        <property type="interactions" value="612"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004102"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004110.1">
        <property type="protein sequence ID" value="ENSLOCP00000004102.1"/>
        <property type="gene ID" value="ENSLOCG00000003455.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693978"/>
    <dbReference type="KEGG" id="loc:102693978"/>
    <dbReference type="CTD" id="55512"/>
    <dbReference type="eggNOG" id="ENOG502QVS2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022168"/>
    <dbReference type="HOGENOM" id="CLU_028243_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6U4"/>
    <dbReference type="OMA" id="EENGHMS"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003455">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061300">
        <property type="term" value="P:cerebellum vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006684">
        <property type="term" value="P:sphingomyelin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09078">
        <property type="entry name" value="nSMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000015">
        <property type="entry name" value="sphingomyelin phosphodiesterase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017766">
        <property type="entry name" value="Sphingomyelinase/PLipase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF8">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="33" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="34">
        <location>
            <begin position="313"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="212"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00050994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00068544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00076605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="638" mass="71182" checksum="F281CC4A070D4CF3" modified="2014-04-16" version="1">MVLYTLPFSSAFLRVLHCFAWGLIFPCYWLLDRLLASFLVTTYEKRQRSEDPCYFLAFCILISTPVYLVLLVASLPFAFLGFLIWAPLQAVRHPYIYSKRGRPDKNRAEQGTAGTPVGEWKAQGKSFCFCTANACLLPDSLARFNNLSDTQGRAREVGKRIRNGASRPQIKIYIDSPTNTSISAASFSSLISPQGGGEGPQRVAGVRRTSSLEYKVESNGEAEKEQEEGGNRDEECVPLNDCPSGVQISISAPEPEGEEDQKGQAANHKPKEGDSGSLDSRTASRESLVKFHVADGTLSNNKLTYHTSVLKKASARKRRHGDDSFDHEISAFFPANLDFLCLQEVFDKRAAGKLKRQLHRYFQYILYDVGRYAWKGCSRFKFLNSGLLLASRYPILDASYHCYPNGRGEDALAAKGALFVKVQVGSTPQDQRIVGYISCTHLHALEGDAAIRCEQLDLLLEWLAEFRKSTSHPSGGQGPEDQVAFDVILGDLNFDNSSSEDKLEQQHVLFTHYKDPCRLGAGEEKPWAVGTLLNPTGLYDEEVSSPESLQKVMENEEGRKEYLVYPTSKNHCSSQKGRKIPLKGSGRRVDYILYTEEGLQQDWKVDVEEYSFVTQLAGLTDHLAVSMRLTVSTGEEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M801</accession>
    <name>W5M801_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M801"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004509"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004517.1">
        <property type="protein sequence ID" value="ENSLOCP00000004509.1"/>
        <property type="gene ID" value="ENSLOCG00000003783.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWGX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063861"/>
    <dbReference type="HOGENOM" id="CLU_1431156_0_0_1"/>
    <dbReference type="InParanoid" id="W5M801"/>
    <dbReference type="OMA" id="ICHLERC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003783">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="24175" checksum="FB7899C9E2C9986F" modified="2014-04-16" version="1">MQLYSSPDYCCPIDPTEKVWSDKRIYAEVTSVQLGVVSMKVSVYKCSVRAKVSWNVDRRIPYQNESCPSYICPINKTRISFSLHEIPNLPEVNLELECFTKLCSEQHCAEGGSVKTSLEVRKSCSQSSVKLCYEFELSAVLGIAFGGFLIGVLLIGALWFIKTHTGYSSGLDVGSTHLSALTGCPCSLVKRQPVPTNTLPSENSSANPSIGSTQSTPTSSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAP0</accession>
    <name>W5MAP0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 53 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the VPS53 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAP0"/>
    <dbReference type="FunCoup" id="W5MAP0">
        <property type="interactions" value="1375"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005449"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005457.1">
        <property type="protein sequence ID" value="ENSLOCP00000005449.1"/>
        <property type="gene ID" value="ENSLOCG00000004547.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015165"/>
    <dbReference type="HOGENOM" id="CLU_007339_0_0_1"/>
    <dbReference type="InParanoid" id="W5MAP0"/>
    <dbReference type="OMA" id="YKFAEAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004547">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.110:FF:000001">
        <property type="entry name" value="vacuolar protein sorting-associated protein 53 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="14">
        <location>
            <begin position="41"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="15">
        <location>
            <begin position="696"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="371"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="795"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="97"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="834" mass="94215" checksum="0E5D8EAEE2F5413D" modified="2014-04-16" version="1">AMEEEELEFADELEAILHLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRLALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVVNVLEHFQKYMGIPQIRQLSERTHRVKAAQSELGTQILADFEEAFPSQGSKRPGGPSNVLRDACLVANVLDPRIRQEIIKKFIRQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGKMFPAEWCMTERVAVEFCHITRTELAKLMRARAREIEVKLLLFAIQRTTNFEGLLAKRFTGSTLSDAAGKKPEPPPPSTNPFLEDEPGSEDSVLEKEEELERPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFRAQGPPKVNSEEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALSTIFQKYLREYAWKILSGNLPKTSSSSGGLTISSLLKEKEGSEVAKFTVEELCLICSILSTAEYCLATTQQLEEKLKEKVDKSLAERINLTGEMDTFSTVISNSIQLLVQDLDAACDPALSAMSKMPWQNVEHVGDQSPYVTSVIMHIKQNVPVIRDNLASTRKYFTQFCIKFANSFIPKFINHLFRCKPISMVGAEQLLLDTHSLKTVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPSVAFVDNYIKLLADGNPETFQKILDMKGLKRSEQSSVLELFRQRLPTPPSGADGGSSLSFSTPTPEQESSRIRKLEKLIKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB31</accession>
    <name>W5MB31_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DCDC1 second doublecortin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB31"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005590"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005598.1">
        <property type="protein sequence ID" value="ENSLOCP00000005590.1"/>
        <property type="gene ID" value="ENSLOCG00000004669.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RKGR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016867"/>
    <dbReference type="HOGENOM" id="CLU_067120_0_0_1"/>
    <dbReference type="InParanoid" id="W5MB31"/>
    <dbReference type="OMA" id="WFISASK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004669">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17155">
        <property type="entry name" value="DCX_DCDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043188">
        <property type="entry name" value="DCDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056415">
        <property type="entry name" value="DCX2_DCDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46302">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46302:SF3">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24478">
        <property type="entry name" value="DCX2_DCDC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCDC1 second doublecortin-like" evidence="2">
        <location>
            <begin position="183"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="DCDC1 second doublecortin-like" evidence="2">
        <location>
            <begin position="291"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="326" mass="36029" checksum="64750DB494F8C8DC" modified="2014-04-16" version="1">MASVRRPASSSTTDSKTSGKTEGSRRPCSASPGSLQSSLEDLLVQKYNERFNATLTSTACHLPRKFLSPYESKTLKECKKRRHSAHLSLGSRPLSTAFAMGSQSRDCHSDENSSVKSNEGASECNSSTSNSINRTGPILPSKGKRPFSAPGSQLRMAKKSSQCIWCSTFQRKSPVFKYKPWIIRVIAYKNGSRCTFARVTAPNMNMLLEECTIKLKLNTAARRVFLANGEEAFEPKDIPHDADVYISSGEVFVDPFKEVKDHLSLAKEASWTMNGVVLPNDAKRGKTKVSLSQRLRRLSEKTAMRVLVFKNGTGRTGYEVTSAKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBG7</accession>
    <name>W5MBG7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBG7"/>
    <dbReference type="FunCoup" id="W5MBG7">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005726"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005734.1">
        <property type="protein sequence ID" value="ENSLOCP00000005726.1"/>
        <property type="gene ID" value="ENSLOCG00000004739.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="InParanoid" id="W5MBG7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004739">
        <property type="expression patterns" value="Expressed in embryo and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="14">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="15">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="20577" checksum="F9B04CBC53DE78C5" modified="2014-04-16" version="1">STSQTLRLPLQRALQHTDTAMAPTVTAAMGYSKEHMTLAIKLRKPIVEKMRRDRINSSIEQLRQLLGQEFLDQQPDSKLEKADILEMAVCYLRRHSQRQPVSAPSGPSAESEGYSRCVQEIKRFLSQDGTKVESQGRLLNHFQHAQLSSEKPKCQRVLPGAPVQHITIKGEPSGISPLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBH8</accession>
    <name>W5MBH8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Glypican 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <topology evidence="1 12">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_015219147.1">
        <property type="nucleotide sequence ID" value="XM_015363661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBH8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005737"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005745.1">
        <property type="protein sequence ID" value="ENSLOCP00000005737.1"/>
        <property type="gene ID" value="ENSLOCG00000004783.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682863"/>
    <dbReference type="KEGG" id="loc:102682863"/>
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    <dbReference type="eggNOG" id="KOG3821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244955"/>
    <dbReference type="InParanoid" id="W5MBH8"/>
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        <property type="component" value="Linkage group LG17"/>
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        <property type="expression patterns" value="Expressed in pharyngeal gill and 9 other cell types or tissues"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:side of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell migration"/>
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        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
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        <property type="term" value="P:regulation of protein localization to membrane"/>
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        <property type="entry name" value="Glypican"/>
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        <property type="entry name" value="Glypican_CS"/>
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        <property type="entry name" value="GLYPICAN-5"/>
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        <property type="entry name" value="GLYPICAN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867892" evidence="13">
        <location>
            <begin position="27"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005737.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="567" mass="63362" checksum="9D156D8D70EC5284" modified="2014-04-16" version="1">MGPRRLFCCLVLAVFFGLSLESPTSCDEVRKVFELRQIGPVKSLPEFPITGSDLQVCTSRNVTCCTKKMEEKYQVAARHDIQHLLQTSSSSLKFLISRNVAAFQETFETLIRHAENHTNTLFRTTYRNMAADAAGSVKELFTDVGLFVLGSEVSVEDFVNRFFDSLFPLVYNHLINPGLTDITINYAECIRMTRRDLNPFGNFPKLAVGQIGRSMLPGRVFLQALNLGIEVINTTDHLQYTKECKRALLKMQYCPHCQGLTQSKPCMGYCLNVIRGCLANMAEIDSHWREYVRSLEEVSNSMHGSYDVEQVLLNLHSLVNDAIMHAQINGPKLSVQVHKMCGHPNRKSAQSSGSPLENAKDLVSLKVPPRNTEESLASRRKEFINSLRLYQTFYAGLADHLCVSELASSDGLSCWNGADVVKSYTLRVVGNGIKAQMANPEVKVKGTDPLINQIIDKLKHINQLLQGKSIPKYGSLDLIETGSGNHEGQISGDCDDEDGCSGSGSGEMNKVLQVTKRIPEKVNDNRRRTQQTENVEIMDRSGTHSRKEANIFTFNLICVISFLLNVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC55</accession>
    <name>W5MC55_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein flightless-1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005964"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005972.1">
        <property type="protein sequence ID" value="ENSLOCP00000005964.1"/>
        <property type="gene ID" value="ENSLOCG00000004957.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156643"/>
    <dbReference type="HOGENOM" id="CLU_002568_1_0_1"/>
    <dbReference type="InParanoid" id="W5MC55"/>
    <dbReference type="OMA" id="CFHGWSA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004957">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11280">
        <property type="entry name" value="gelsolin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000033">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000050">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000019">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000020">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000030">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000031">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000034">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000021">
        <property type="entry name" value="protein flightless-1 homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="342"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="464"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="593"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="901"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="1006"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="784"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1097" mass="126607" checksum="7E9B12ADF87E39D4" modified="2014-04-16" version="1">MAATGVLPFIRGVDLSGNDFKGGYFPEHVKSMTSLRWLKLNRTGLCYLPEELASLQKLEHLSVSHNSLTTLHGELSSLPCLRAIVARANNLKNSGVPDDIFQLDDLSVLDLSYNQLTEIPRELENSKNMLVLNLSHNSIENIPNQLFINLTDLLYLDVSDNKLDSLPPQMRRLVHLQTLILNNNPLMHAQLRQLPAMVALQTLHLRNTQRTQSNMPTSLEGLTNLQGKDSADCFINREKKFDSIFKRPHVNLTFFSVYGFIKXRRRKDSSQDDQAKQVLKGMTDVAQEKNKSIEENGDMKYGDLKTRRWDKNLEKPQLDYSEFFMEDVGHIPGVTVWQIENFIPTQVDETFHGKFYEADCYIVLKTFLDENSSLNWHIFYWIGQEATLDKKACSAIHAVNLRNYLGAECRTIREEMGEESEEFSAVFGNDISYIEGGTASGFYTVEDSQYVTRNRLFRVYGKKNIRLESVALKTSSLDPRFVFMLDNGLEIFVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLGHNQETPEFWELLGGQPEEIKKHVPDDFTPVRPKLYKVGLGLGYLELPQINYKLSVEHKEKLKLDVVPEMRLVQSLLDTKSVYILDCWSDVFIWIGRKSPRLVRAAALKLGQEVCSMLHRPKHAMVIRNLEGAECQVFKSKFKNWDDVLKVDYTRNAESVLKTKGLTGKVKKDAEQKDQMKADLTALFLPRQPPMPLAEAEQMMEEWNEDLDGMEGFVLEGKKFARLPEEEFGHFHTQDCYVFLCRYWVPVEYEEDPKKGDKDKGNEEAEEEEKQPEEDFQCVVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENLKFLSHFKRKFIVHKGKRKLKDNNVQPSLYHIRTNGSALCTRTIQIPTDSSNLNSEFCFILKVPFESTDNQGIVYTWVGRAADPDEAKLAEDIMNTMFDDTYSKQVINEGEEPENFFWVGIGSQKPYDEDADYMKYARLFRCSNEKGYFSVSEKCSDFCQDDLADDDIMLLDNGKEVYMWVGTQTSQVEIKLSLKACQVYIQHMRSKDPEHPRKLRLVRKSNEPHNFTRCFHAWSSFKKPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDT3</accession>
    <name>W5MDT3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor GTPase-activating protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Zinc finger protein 289</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01036572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDT3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006542"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006550.1">
        <property type="protein sequence ID" value="ENSLOCP00000006542.1"/>
        <property type="gene ID" value="ENSLOCG00000005421.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0706">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155568"/>
    <dbReference type="InParanoid" id="W5MDT3"/>
    <dbReference type="OMA" id="PANQVCF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005421">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09029">
        <property type="entry name" value="ArfGap_ArfGap2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000004">
        <property type="entry name" value="Putative ADP-ribosylation factor GTPase-activating protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF10">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="18">
        <location>
            <begin position="11"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="169"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="356"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="551" mass="60192" checksum="9504E1361AA61285" modified="2014-04-16" version="1">MASEPNKTEILTIFKRLRSIPTNKACFDCAAKNPSWASIPYGVFLCIDCSGIHRSLGVHLSFIRSTELDSNWNWFQLRCMQVGGNANATAFFRQHGCTTNDTNAKYNSRAAQMYREKIRQLADAALSKHGTDLWIDGHGGAQAHTPEKKDSDFFAEHTQVFPANSWDMASLSDENGAVPQGPQAVEESPKLQENIKNQPEEGPSIEGLSTSPKANIEDSMRLASDEPQSSTEVKPSLIGKKKPAASKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKMREQQASEAKKQAEESIVTSMRLAYQELQIDRKTEDKKLQNLEGKKREQAERLGMGFGSRSAVSHSVLSEMQVIEQETPVAAKSSRSKLDMFDEPGFTSGPPKYKDNPFSTGDSFGSRWDSDGGSSFSSWGLEKEEPKDSEVTISSIQPTGDRLPSRRKPDSNVTVVESSEARQKFANAKAISSDMFFGRESDAEYEAKTRLERLQGSTSISSADLFGDGTDHSSAGGSTFESVLPSGPDIAQFKQGVKTVAGKMAVLANGVMNTIQDRYGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDT4</accession>
    <name>W5MDT4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Keratin 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDT4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006543"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006551.1">
        <property type="protein sequence ID" value="ENSLOCP00000006543.1"/>
        <property type="gene ID" value="ENSLOCG00000005422.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQIF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161090"/>
    <dbReference type="HOGENOM" id="CLU_012560_6_1_1"/>
    <dbReference type="InParanoid" id="W5MDT4"/>
    <dbReference type="OMA" id="EMTRNIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005422">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
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                <person name="Alfoldi J."/>
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                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
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                <person name="Lander E.S."/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2137" mass="224743" checksum="C928FCB85458DF7F" modified="2014-04-16" version="1">MSGSSLPACAECAGLQKAAATRFALAFLCAACQVMERNSNHVQDNVIECTDSAGEQIREGFISCHCLEKDHFPAGPGKSHQELEAVATKEAEEKQNRDIVQEKEGQLMEERKKRKDDKKKKDAGLKKAIEQKNKVPEHIKSSLSQPQPANPNSGTSTVTSTVSNAKRAPASSQPQAPPRYPPREVPPRFRQHEQKQLLRRGQPLPVLAATLGTRAQPSSGQPGGAPPASEGSLQNGTKAHHPGMPPIRDLVSHSPNQTDLNRSGLGSHYENSHWGSGSTGSDSNTNWDKVIVDGCDKEAWPSITGSDPELASECMDTDSASSSGSEKNLSIMASGNTGGDGDGSRHSSGHGSHFMVANGSNNVGNGSIKGPWGSSSGSMLSTCQGPGENPNSKLTESSHGKLNAWGTLGSSTNGGNPSTLNPNANHGAWPVLENSGHNQHGSVGNGNGNTSIQRSTIGQTPGSQSINSKGGVSTHASWGSLQDNTPESEVNGTSKVSLSGQPQNLNTEMNGPNNTTNLMTSSLPNSTGSMQMNELPTGHRAWRVGTGSSTQLQASSVSNGTSISQQGNSEGMNGTAWGAPPGTNYSGDKCPVPKGQAVGDTVNATLMQSGINGSAAGAASFKNNNNGVGGRGGGWDSGAAWGAGNGVGPVGIPRPWGSASSSSSSSSSSSSSNTGTKVSKGEWNSLPNNNQHSNDGGNGSRKGTNGWKSLEDDALGVGGSNGQAPQSSEQGGMWAKSGGSEGSGESTGSRSDRMSGDAPRGRDRRKANPQGPVQNVISRSDLDPRVLSNTGWGQTPIRQNTAWDIEATSCNDKKTDNGTEAWGGSVSQASDSGGWGDGPSANSNDTSSVSGWADPKPAPGWGDTKGSSGQGGWEDNSAATGVGKSNQSWGGSKEDKSSTWSDAQKVKQGWMEGQKSNQGWGTPSGAGWGGDVPRGNHWGDPPKSGSGGWDSDSDRSGSGWSETGRAGGGNTWRGSGGGNSPNTAGPSGWGDPNKANNQGQGWGEPPKPSHPAQGWGDTGKPRNSPDWNKPQDATAGPPSSWGTGTSQPGPSTNNKPSGWLGGPIPAPPKEEESTGWEEPSPESIRRKMEIDDGTSAWGDPSKYKYNNVNMWNKNSIADQETQASSQQHPPQPLPPPSAIPSKEKGWGEPYRAPPKAESSWGEPTGPPVAVDNGTSAWGKPMDTGTSWEEAGVESAGTGSWSGPAVGQQPPHKPGPKPMQDGWCGEEMPMPSMRHSSWEDEEDVEIGMWNNTPSQEMNQSGNWPSCMKKLPPKGNIKGGNKQDDVWINQFVKQFNNIGFSRESSDEPLKSNKMELPGGMLPDKRMDMDKHGLNIGEYNGVIGKSPGSRHQISKESSMERSPYFDKLSLSLSGQDGIVAEEPQNVQFSSNQNMKLPPSNNALPNQSPGSVSGLSLQNLNSVRQNVNPGFSGSNAAAQARSTQQPPAQPLNSSQPNLRAQVPPPLLSPQVPASLLKYPPNNGGLNPLFGPQQVAMLNQLSQLTQLSHISQLQRLLLQQKVQNQRSMPIGGRQQQDQQGRPLGTAHQMMPPPRHLDPSLMKQQQQQQQQPSPQPSSLHQPGLKSYLENFMPHNASDLQKEQNTLNSFSNFPIGGFPSHPNLGQADSLLHQAVKHPLSGSDGSGCYPAGSSHSHSLFSPPLSNGHAGPGSPIYHRAGRTFASNENHTLSTRGLNSNLNVSNLDIGSISFKEPQSRLKKWTALDISVNPSLDQNSSKPGAIPSGLRLEDSPFGPYDFMNTSNSPVSPPGSVGDGWPNRAKSPHGSSNVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVRDVDHLRDRNNGSTSSLNTTLPSTSAWSSIRASNYSGSLSSTAQSTSARNSDSKSTWSPGPVTNTSLAHELWKVPLPPKGITAPSRPPPGLTGQKPPSSWDNSSLRLGGGWGSSDSRYTPGSSWGDNSSGRTTNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNAVVCYSSKEEAAKAQKSLHMCVLGNTTILAEFASEEEISRFFAQGQSMTASPGWQALGVSQNRIGSIESSHPFSNRNDPNHWNGAGLSGAGSGDLHGTSLWGASNYSTSLWGNPSGSEGRGIGSPSPINSFLPVDHLTGAGDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MER0</accession>
    <name>W5MER0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaJ (Hsp40) homolog, subfamily C, member 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MER0"/>
    <dbReference type="FunCoup" id="W5MER0">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006869"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006877.1">
        <property type="protein sequence ID" value="ENSLOCP00000006869.1"/>
        <property type="gene ID" value="ENSLOCG00000005690.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159907"/>
    <dbReference type="HOGENOM" id="CLU_017633_13_3_1"/>
    <dbReference type="InParanoid" id="W5MER0"/>
    <dbReference type="OMA" id="CCEPATP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005690">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF7">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="113" mass="12268" checksum="AE3FD9E7653DB531" modified="2014-04-16" version="1">GATLITVGLGVAAAAFAGRYTFHLWKPLGQVITETAKKMPASSFSSYYKGGFEQKMTRREASLILGVSPTSNKTKIREAHRRIMVLNHPDKGGSPYLAAKINEAKDLLDSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFD1</accession>
    <name>W5MFD1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Outer dense fiber protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cenexin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Outer dense fiber of sperm tails protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ODF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFD1"/>
    <dbReference type="FunCoup" id="W5MFD1">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007090"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007098.1">
        <property type="protein sequence ID" value="ENSLOCP00000007090.1"/>
        <property type="gene ID" value="ENSLOCG00000005870.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694642"/>
    <dbReference type="CTD" id="792921"/>
    <dbReference type="eggNOG" id="ENOG502QUXQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063497"/>
    <dbReference type="InParanoid" id="W5MFD1"/>
    <dbReference type="OMA" id="HVHINDT"/>
    <dbReference type="OrthoDB" id="413404at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005870">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026099">
        <property type="entry name" value="Odf2-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162:SF8">
        <property type="entry name" value="OUTER DENSE FIBER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="11 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="115"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="320"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="531"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="675"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="847" mass="96361" checksum="B2F99BBBF3674226" modified="2014-04-16" version="1">MRRAMKTRSSSPPLHVHVDDSTPVHVHVRKCPKTSPSKAAQLKIQEAQTKKDAGNLRATARVKTRVPWIPPGKGSLRESAYKWEGPTHRLEITPAAEPEPGQAALRLADLSTDEEEALHGRISQYERKIDSLMNEVGSLKSEVELRKKEQLLERRAEQLSASQKVIEEQEEELAEVTKELAVTERENSLLRHSIEKMKEETDYSSSDHLKGQDSNGLEKQHLLLEKDALLKKLVEAEMDGAAAAKQVSALRETIGRMRTEKRMSSSDASLLARQKELLMQKLETFESTNRTLRHLLREQHGRETDAIRLSEQKEVLLKKLADNEADNTRLLLKLQEKEKELDQLQTQLETEKENARTTGELSKALESTRAHLQGQLRSREAENNRLGVQIRTLERTLTQQQGEMEHLLEQLRELKQRADADKEALKRATRAQKQRAERSEDTAGQLSAQLMEKETQLADALSASESWRSRHAQAVKDKSQLEIEVTVLNNRVTDLMDQLHGAEDKSRAEREGLLDRLHRLTSESTAGRLESQRLKASLSAVEEKLTLAQSEVQQLKASVKQYESLVESYKTQVHKTRAEADEYCLRLQQAGQDGQAARAELGREVELVRGQLLGRLAELEPLPELLRRRELELQAAQAQAQACERRSAEQAAALAELRGKVEQQGGQMEGVRERNMALLEENKQLHLRVDSLERKLEEANAQNRDLVQVIAKREETIHNNQLRLEEKSRECSTLARQLEGALEDARRQVDQTRERAVSKERAAQGKVLDLETQLSRTKTELNQLRRTKEDADRRFQSRLQDMRDRLEQSDSTNRSLQNYVQFLKASYANVFGDPALTSSALRPPSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFN3</accession>
    <name>W5MFN3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA fragmentation factor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of DFFA and DFFB. Interacts with H1-1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFN3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007192"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007200.1">
        <property type="protein sequence ID" value="ENSLOCP00000007192.1"/>
        <property type="gene ID" value="ENSLOCG00000005950.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R0RF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014490"/>
    <dbReference type="HOGENOM" id="CLU_049235_1_1_1"/>
    <dbReference type="InParanoid" id="W5MFN3"/>
    <dbReference type="OMA" id="KAEHVEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005950">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:DNA nuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000006">
        <property type="entry name" value="DNA fragmentation factor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039729">
        <property type="entry name" value="DFF40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015311">
        <property type="entry name" value="DFF40_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067:SF2">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09230">
        <property type="entry name" value="DFF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="12">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="336" mass="39102" checksum="1C99E8CF34C49831" modified="2014-04-16" version="1">SNMFGFFKKNKLVKLRGFGSNQKYGMAATSVKELLKKGCETLQVPHSGSRVCLYEDGTEVSEDYFKKLPDNTELILLAAGQTWNGFVCDVGLLLGASDLHTELLIESARGLLTDEHAPKRRKILQELLHNLKETSDLESREEDEDWFEGVDSRFKTKSAYMKYNCENRVRGYLKEVDGYTQHIKCPRARGEYKKTVDMMTEKLKAIKYNGCYFDRKEKEPNRLCTEEGWFSCQGAFDQDECLSLHSINPYGNRESRILFSTWNLDHRIEKKRTIIPTLAEALKKHKSSEINLDYFYRLLFTRENLKLVHIVCHKKSVHDLVCDKNKIYKKGKRRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH57</accession>
    <name>W5MH57_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase 25</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Interacts with CTTNBP2NL. Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. Interacts with TAOK3 (via N-terminus); the interaction promotes STK25 abundance at the level of protein expression and/or stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01015230">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637735.1">
        <property type="nucleotide sequence ID" value="XM_006637672.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH57"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007716"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007725.1">
        <property type="protein sequence ID" value="ENSLOCP00000007716.1"/>
        <property type="gene ID" value="ENSLOCG00000006383.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685622"/>
    <dbReference type="KEGG" id="loc:102685622"/>
    <dbReference type="CTD" id="449653"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153476"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="W5MH57"/>
    <dbReference type="OMA" id="YNMYIVM"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006383">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
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        <property type="entry name" value="Serine/threonine-protein kinase 24"/>
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        <property type="entry name" value="Serine/threonine-protein kinase 25"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="23"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
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            <begin position="49"/>
            <end position="76"/>
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            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
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        <location>
            <position position="52"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
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        <source>
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        <source>
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            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="427" mass="48420" checksum="D848850FCFCCF027" modified="2014-04-16" version="1">MAHLRDLRDLRNQNSRLDPEEYFTKLERIGKGSFGEVYKGINNRTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPFVTKYYGSYLKGTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGSYSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTPYLTELIDRYRRWKSEGHGDESSSDDSDMDGDGDEREQCPVWTFPTVRPSSLNKLQNGLSLPNSQNAEAVKRQPKSQCLSALVTPIFRELKEKRRASGGSLGAIEELENAFNLAEESCPGISDRLVTHMMERVRRFSLNGNTTPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHD3</accession>
    <name>W5MHD3_LEPOC</name>
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        <submittedName>
            <fullName evidence="6">Si:ch211-222n4.2</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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    </organism>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005173">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000006453.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_986818_0_0_1"/>
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        <property type="component" value="Linkage group LG20"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="entry name" value="CCDC74_C"/>
    </dbReference>
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        <property type="entry name" value="CCDC74A/CCDC74B/CCDC92"/>
    </dbReference>
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        <property type="entry name" value="CCDC92/74_N"/>
    </dbReference>
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        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 74A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14882">
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            <begin position="7"/>
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    </feature>
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            <begin position="163"/>
            <end position="286"/>
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            <begin position="70"/>
            <end position="85"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14917"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSLOCP00000007792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="32190" checksum="573EBBDCAF8A3F68" modified="2014-04-16" version="1">GMESEPRRVTALERDLQFLQQQHTETLGKLHAEIEQLRRENKVTDLQYKLIMEPKQSRKGKTANHSNFLRSSGRSSTTSSASQSGHRLSQQPSGEWDFKTALLEPHGWHPVLLIFSVQHCNNTDSGTGSGGENEVACDAAACPEQDLKGGLITSLQPLRILSSPLQPPRAPTLQECEVIIRQLYNANSLQSQELLHLKSVLKDIILNNNKITPETYILTKAYLTGTSRVPSLVEGSKCFSFHCARPDAQPSAAERVMLPALKQSLGTSFAERQKRTQAMQKNRLRKVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIE2</accession>
    <name>W5MIE2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translin-associated factor X interacting protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIE2"/>
    <dbReference type="FunCoup" id="W5MIE2">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008151"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008161.1">
        <property type="protein sequence ID" value="ENSLOCP00000008151.1"/>
        <property type="gene ID" value="ENSLOCG00000006745.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683481"/>
    <dbReference type="KEGG" id="loc:102683481"/>
    <dbReference type="CTD" id="55815"/>
    <dbReference type="eggNOG" id="ENOG502QTWK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165060"/>
    <dbReference type="HOGENOM" id="CLU_028246_0_0_1"/>
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    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006745">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032755">
        <property type="entry name" value="TSNAXIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306:SF0">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15739">
        <property type="entry name" value="TSNAXIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translin-associated factor X-interacting protein 1 N-terminal" evidence="4">
        <location>
            <begin position="110"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="333"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="704" mass="80494" checksum="17B6843204E29211" modified="2014-04-16" version="1">MFGMSVQKDCKLPPLPVSERQGLPVNCPGGSEKPVRKSRQQSDEIGSLFTLKTAHGAGATGFLSTWPAHVSSQIVHQGHRQPPGSENRIRGCGEELSGLVSKPRFLEQLESYLRRELQALDSSQLDAPERRLQAYQEVFQYFIEDLKTYKPLLSAIKNEYEIALAHLREQIRTLEPLKGMLVVVSEQCDQRVLALREEERAEVKALKQEKSHLLMIIDNMNESKNALQAQVSRLQEDLAAQYLLYREESDARKLLVNDISNMRYLQEEHKSPEQEVEEQEDAVRVRLALKVAREDLTRLQVELNRMQAEYGDVVPRRDWESLDRKHRDTLGKMEMLQKDFSQMKQEYDTLLEVHRQLAEQREGLQAELERFRGSSTPRPRWEKCADVVSGGSERWAQLTEGQSSDQLVDILLEELRGGPAKEKDFFDGLGTGEDVPVYLRYEGQIRNLKLKKSEIVNTIKEVWKDKVLEDEQKEERQNLAEFLHSFLEKRHVDGATEWAYSLVEGCRRHQDDDFISLFFSILLGKVDESVYHGQIHLLSHLLKELIHSDAAESSTLTMQEFSDALRRAFPLKMEAQIEELIQAAQAQLGSTFTYQALFTEDAEGKPGPFLKLVKKQANAEKHKYLTELRAQLGSKEQVEVADLKTAFQSIDPSLDSQILDRYLSVAFQTRSDRLEQAAPVDTDSALQRLLAAHVKRAGPLPPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ21</accession>
    <name>W5MJ21_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ21"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008380"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008390.1">
        <property type="protein sequence ID" value="ENSLOCP00000008380.1"/>
        <property type="gene ID" value="ENSLOCG00000006928.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQB2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244894"/>
    <dbReference type="HOGENOM" id="CLU_440716_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJ21"/>
    <dbReference type="OMA" id="WMANTEL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006928">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF42">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="9">
        <location>
            <begin position="556"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="620" mass="69872" checksum="510BB3C0440B5FF3" modified="2014-04-16" version="1">MSYYSQGGVLNPAYHQSETLEMDRSSSRTAQPLNPYDSSGPGGQRDRGAGGLSSTGMLPPYGEEGGYDTLPMRPLPPQTRPPLEHRGQTNRGFERDSVIEYNSGPQDWYQGRHHVDYQDQPDYSRREGGALRRRASRRRSLFPAGTLSATALGRLGLTAEDFREEIEREEQDLVTELAAKSIRDRVRAIRQLPMSLQDKSHIRKQVLLLVTSKRRLQLTCCTDCTESVSLFFRRLGGHLSSVRQALELWSGTLKEIGGRFGSSVLSFFTFLKWLLMFNVFSFLINFGFITVPQLLEPPGGPNVSFRGLEILTGSGYFSYTVLYYGYYSNGSVSGSSAGSSQNSTLPQYDMRLAYFFTMGTYLVLCGLCLVYSAARSFRENFMLGGELSGRAWRLLCSWDFSVVNEKAVQLRQRNLQIQLKESLSESAQKQAVSSLSEKLSHFGIHLGIWLISLSLALGSCAAVYFLCLFNLQLIENAQTSSSLQDEASTLLLPIVVSLINLLIPLLYTVLRKIEPFTVQRHQIYTLILRNVILKMSIVGVLCYYWLEKVTNSKIQCWESFVGQDLYRLVVIDFFFCLIGSFFGEFLHRVIGVRCVKSLGVPEFDIARNVLDLIYAQTLAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJJ6</accession>
    <name>W5MJJ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01040103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJJ6"/>
    <dbReference type="FunCoup" id="W5MJJ6">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008555"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008565.1">
        <property type="protein sequence ID" value="ENSLOCP00000008555.1"/>
        <property type="gene ID" value="ENSLOCG00000007066.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S34U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164599"/>
    <dbReference type="HOGENOM" id="CLU_049894_10_1_1"/>
    <dbReference type="InParanoid" id="W5MJJ6"/>
    <dbReference type="OMA" id="NDHTCFT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007066">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866362" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="19365" checksum="7A3C3E5B56B809B3" modified="2014-04-16" version="1">MKAVLVLLVCVATVCALDVWQHCSARFPQACPIQKYKDWYSVGSLCVKYYNTALNYSDAEFACRKEVPGGHLVSVHSNQSNTDLLCVVMKYNNTNPRIWLGAFELFRTGLFVWTDGTDWNYWNWVPGQPDNTANAEDCVEMNWSDTGKWNDDRCFYKKNYICAFKNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJM8</accession>
    <name>W5MJM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008587"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008597.1">
        <property type="protein sequence ID" value="ENSLOCP00000008587.1"/>
        <property type="gene ID" value="ENSLOCG00000007094.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ46">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244866"/>
    <dbReference type="HOGENOM" id="CLU_2391651_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJM8"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007094">
        <property type="expression patterns" value="Expressed in larva and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF6">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="94" mass="10570" checksum="853C66EE0E4E6BD9" modified="2014-04-16" version="1">MEICLWMLNASPKFKRDPGEDCSARCNPIYVSRIVSAMINSNDDNGVLVGKWDDDYKDGVKPTSWSDSVSILRKWHKSGGQPVKYGQCWVFAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJR5</accession>
    <name>W5MJR5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7</fullName>
            <ecNumber evidence="27">1.1.1.210</ecNumber>
            <ecNumber evidence="15">1.1.1.270</ecNumber>
            <ecNumber evidence="16">1.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">17-beta-hydroxysteroid dehydrogenase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">3-keto-steroid reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Dihydrotestosterone oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Estradiol 17-beta-dehydrogenase 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>17beta-estradiol + NADP(+) = estrone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:24618"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-dehydro-4alpha-methylzymosterol + NADPH + H(+) = 4alpha-methylzymosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:36379"/>
            <dbReference type="ChEBI" id="CHEBI:1949"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136486"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36380"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NADP(+) = 4alpha-methyl-5alpha-cholest-7-en-3-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18409"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16495"/>
            <dbReference type="ChEBI" id="CHEBI:18378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:18411"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>4alpha-methyl-5alpha-cholest-8-en-3-one + NADPH + H(+) = 4alpha-methyl-5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46832"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
            <dbReference type="ChEBI" id="CHEBI:87051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:46833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>5alpha-androstane-3beta,17beta-diol + NADP(+) = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16297"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:18329"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.210"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:16299"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>5alpha-cholest-8-en-3-one + NADPH + H(+) = 5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46852"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 3beta-hydroxysteroid + NADP(+) = a 3-oxosteroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36836"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:34789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>zymosterone + NADPH + H(+) = zymosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:33460"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Steroid biosynthesis; estrogen biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="26">Binds to the short form of prolactin receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.210" evidence="27"/>
    <dbReference type="EC" id="1.1.1.270" evidence="15"/>
    <dbReference type="EC" id="1.1.1.62" evidence="16"/>
    <dbReference type="EMBL" id="AHAT01015973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634994.2">
        <property type="nucleotide sequence ID" value="XM_006634931.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJR5"/>
    <dbReference type="FunCoup" id="W5MJR5">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008624"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008634.1">
        <property type="protein sequence ID" value="ENSLOCP00000008624.1"/>
        <property type="gene ID" value="ENSLOCG00000007122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696390"/>
    <dbReference type="KEGG" id="loc:102696390"/>
    <dbReference type="CTD" id="51478"/>
    <dbReference type="eggNOG" id="KOG1478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013340"/>
    <dbReference type="HOGENOM" id="CLU_029944_2_0_1"/>
    <dbReference type="InParanoid" id="W5MJR5"/>
    <dbReference type="OMA" id="WHNIDGY"/>
    <dbReference type="OrthoDB" id="9989144at2759"/>
    <dbReference type="UniPathway" id="UPA00770">
        <property type="reaction ID" value="UER00758"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007122">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047024">
        <property type="term" value="F:5-alpha-androstane-3-beta,17-beta-diol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006703">
        <property type="term" value="P:estrogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08941">
        <property type="entry name" value="3KS_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000289">
        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052834">
        <property type="entry name" value="3KSR/17beta-HSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042829">
        <property type="entry name" value="HSD17B7/Erg27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE_17-BETA-HYDROXYSTEROID DEHYDROGENASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="3 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 33" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00063141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00066807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00071031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00077091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00083156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00083257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="41154" checksum="63150D93C00FAC42" modified="2014-04-16" version="1">MLFCFRGCEFPMSKMEKVVLVTGANSGIGLALCERVLLHSSRIRLCLACRNMRRAEAARAGLLVAHPGADISILRLDVSSVKSVLEAAKEIKKRYQRLDYLYLNAGIMPNPQISFKAFFNGLFSRKAISMFATGEGLLTQQDCVTSDGLQEVFVTNLFGHFLLIRELEPMLCQPGRTSQLIWTSSSNARRSAFSLEDYQHSQGVEPYSSSKYASDLLSVALNRHYNKQGLYSTVICPGLVMTNLTYGILPSFFWTLILPIMWLIRIFTNTFTLTPYNGAEALYWVFIQKPEALDPRAKYHSLTSGFGNSYTEPKKMDIDEEMAEAFYVELLRLENQVLRNITQEKRNSSIRLQKAQRNQDFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJY1</accession>
    <name>W5MJY1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">adenosylhomocysteinase</fullName>
            <ecNumber evidence="7">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine. Binds copper ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21709"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="11">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01024219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJY1"/>
    <dbReference type="FunCoup" id="W5MJY1">
        <property type="interactions" value="1478"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008690"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008700.1">
        <property type="protein sequence ID" value="ENSLOCP00000008690.1"/>
        <property type="gene ID" value="ENSLOCG00000007175.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182981"/>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_1"/>
    <dbReference type="InParanoid" id="W5MJY1"/>
    <dbReference type="OMA" id="YIGVTVE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007175">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="13">
        <location>
            <begin position="194"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="225"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045926"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="435" mass="48032" checksum="DF7BCC7CD9D85056" modified="2014-04-16" version="1">TNMSEKLPFKVADISLADWGRKAIEIAENEMPGLMKMRELYGESKPLKGARVAGCLHMTLQTAVLIETLRALGAEVQWSSCNIFSTQDHAAAAIAKAGVPVYAWKGETDEEYLWCIEQTIYFKDGQPLNMILDDGGDLTNLVHKKYPKLLAGIKGISEETTTGVHNLYKMLKNGELKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCVQALRAFGARVIVTEIDPINALQASMEGYEVTTMDEACKEGNIFVTTTGCEDILLGRHFEQMKDDAIVCNIGHFDIEIDVKWLNANAQDKVNIKPQVDRYKLKNGRHIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVLAQIELWTNTSKYPLGVYFLPKKLDEEVAAAHLDKLGVKLTKLSEKQAKYLGLPLAGPFKPDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK30</accession>
    <name>W5MK30_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">B cell linker</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202191.1">
        <property type="nucleotide sequence ID" value="XM_015346705.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008739"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008749.1">
        <property type="protein sequence ID" value="ENSLOCP00000008739.1"/>
        <property type="gene ID" value="ENSLOCG00000007223.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690671"/>
    <dbReference type="CTD" id="29760"/>
    <dbReference type="eggNOG" id="ENOG502QUXR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155715"/>
    <dbReference type="InParanoid" id="W5MK30"/>
    <dbReference type="OMA" id="ERPTHDY"/>
    <dbReference type="OrthoDB" id="10044490at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007223">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09929">
        <property type="entry name" value="SH2_BLNK_SLP-76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000016">
        <property type="entry name" value="B-cell linker protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF3">
        <property type="entry name" value="B-CELL LINKER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="417"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="526" mass="58928" checksum="B42B426DC733A23B" modified="2014-04-16" version="1">MDKMRQLQKMVQDIKKNDGGIMNKFKKFQNEQVAFIRKTGKNTWDRITNKPPPTVPRRDYPDEDDEDEQWSETEFDSDYENADDHSETYEVPLEDNDDSYEPPPCEKEKRNITSVSSLSKGDYADKRTESSRLPPRKPVPSIPRPQQPVSPQKPPKPQLLVKPQKPEQDDADYICPKEDGEDDNYIEPSEKSSVQDPPAVNRDTKPTIKRSPPPGGTHEPTELYEVPDLEVIPSAFSRVNAPTPPKAQPRSAGRDGPLPISPRPPIKKLPAASEPVDEDEYEVCDPDNSESSLKSQPSTLHSLKTPAPLPREGKKPILPVKPEDQSSGSLPRKDTDEARKPVMNWEGDVKARPTAMKHNNLNTATDLPPPAARAKPQLSKGMSTTPPKLPSTVARGSLPRNGPTAAEQEAGVYSKSWYASTSERKVAEDALMKTNKDGSFLIRKSSGHDAKQPYTLVVFYKKKVYNIPVRYIESSKQYALGREKAGEEWFSSVADMIENHQHNPLVLIDSQNNTKDSTKLKYPVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLM8</accession>
    <name>W5MLM8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 45 member 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-associated sucrose transporter. May be able to transport also glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>sucrose(out) + H(+)(out) = sucrose(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018163">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLM8"/>
    <dbReference type="FunCoup" id="W5MLM8">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009287"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009298.1">
        <property type="protein sequence ID" value="ENSLOCP00000009287.1"/>
        <property type="gene ID" value="ENSLOCG00000007657.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696067"/>
    <dbReference type="KEGG" id="loc:102696067"/>
    <dbReference type="eggNOG" id="KOG0637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182914"/>
    <dbReference type="HOGENOM" id="CLU_015081_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLM8"/>
    <dbReference type="OMA" id="VPNINGS"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007657">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000069">
        <property type="entry name" value="Solute carrier family 45 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000328">
        <property type="entry name" value="Solute carrier family 45 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF7">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="412"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="755"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="800" mass="87974" checksum="3EDC7D27FC1F3716" modified="2014-04-16" version="1">MLTSMAPQNADSDTMQVENIAVAPVKKQGSSGREVESQDESVSEGSIDRIPMRRWVMHGAVMFGREFCYAMETALVTPVLLQIGLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLRWGRRRPFILALCVGVLLGVALFLNGSVIGLALGDVLNNQPIGIVLTVLGVVVLDFCADATEGPIRAYLLDVADTEEQDMALNIHAFSAGLGGAVGYMLGGLDWTHTFLGQIFKSQEQVLFFFAAILFTISVGLHLFSIEEQQYSPQQDRLDEEADTMSTVKLNGSLVAPALPPLDLIGEEQPYRLYNEAHSEHDMDMDFLDVEIVRSKSDSVLHVTDATLDLDAEALFLCDIEPSIFQDHLSSYHSTPPRRSSSAGSQEFLRAKFGRLSDFLHENERDGQETLLNNQLLEAKAPNGGLAGADTNGLHGQARTGMKQSNTSASMRRRRHTFYRQPSCTFSYYGRVGTHRYRYRRANAVVLIKSSRSLNDIYEMQKRQKKLRQRQRHQSGNTNSSGDTESEEGETETTVRLLWLSMLKMPPELLRLCLCHLLTWFSIIAEAVFYTDFMGQVIFEGDPTAAGNSTALQNYHKGVQMGCWGLVIYAATAAICSALLQKYLDNYDLSIKIIYILGTLAFSIGTAVMAIFPNVYVSMVMISSMGIISMSISYCPYALLGQYHEIKEYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGMVDAVGSVRVIPMVASGGSFLGFLSAAFLVIYPSSASEEPKEEQKKALEPGEGSGASPTEKPSVLKLTRKGTTTTTCKVECESTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN87</accession>
    <name>W5MN87_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Growth arrest-specific 2 like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GAS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN87"/>
    <dbReference type="FunCoup" id="W5MN87">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009846"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009857.1">
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    </dbReference>
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        <location>
            <begin position="26"/>
            <end position="154"/>
        </location>
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        <location>
            <begin position="219"/>
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        </location>
    </feature>
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        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
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        <location>
            <begin position="295"/>
            <end position="502"/>
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        <location>
            <begin position="522"/>
            <end position="650"/>
        </location>
    </feature>
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        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
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        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
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            <end position="479"/>
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        <location>
            <begin position="534"/>
            <end position="569"/>
        </location>
    </feature>
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        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038441"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
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        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009846.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="650" mass="71795" checksum="F3E69B6F35BD4E75" modified="2014-04-16" version="1">MADQSNIQSAASKSIRPFKSSEEYLYAMKEDLAEWLNTLYDLEITADTFMEGLETGGALCRHANNVNRAALDFQLENPEAARSMRVPRKDVVFQPRSVAPGSFVARDNVSNFIAWCRQELWIQDVLMFETNDLVLRKNEKNFVLCLLEVARRGAKFGMLAPMLIQLEEEIEEEIRDQESLAGGAQRSPPSRGYLREENPGVPLPGVPVYPSWPQRRVLCDMRNLDELVREILGRCSCPSQFPMTKVSEGKYKVGDSSALIFIRVLRTHVMVRVGGGWDTLEHYLDKHDPCRCNAFDSGTPLRTPDRRTLEPPGAGRTPRHSLPTELDASTGRAPATPRPPRDRSEPRQAPPLRSKEALPVVTRKLSGDSDSSTASSKGGGGRPSLPGSKKPGEEVVLLVSRREGKHVIERPGATHVPALRPPQPRARSQSRGRSVILRPSPPLDIPKPDSIKARPDRGRSLGPESPRKLQTSRSQSQGKPFSRGRDSEANVAQPRALPCLDPSREKELYRSFEAEFLANTQRSGFAEGGDDYGDLLAQPLQPQALQQPPLGDPNVTDSAYSSSNSSTSSLNVGNKLGVLPDLRDSTRATEAQAGGCGQSHTADPCVVLRPKKGLKKPERVPSIYKLKLRPKLRPRTDNRPEKSPSRIPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNL2</accession>
    <name>W5MNL2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RWD domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Enhancer of SUMO conjugation. Increases SUMO conjugation to proteins by promoting the: binding of E1 and E2 enzymes, thioester linkage between SUMO and ube2i/ubc9 and transfer of SUMO to specific target proteins which include hif1a, pias, nfkbia, nr3c1 and top1. Has no effect on ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNL2"/>
    <dbReference type="FunCoup" id="W5MNL2">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000009983.1">
        <property type="protein sequence ID" value="ENSLOCP00000009971.1"/>
        <property type="gene ID" value="ENSLOCG00000008213.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688106"/>
    <dbReference type="KEGG" id="loc:102688106"/>
    <dbReference type="CTD" id="25950"/>
    <dbReference type="eggNOG" id="ENOG502QSYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000954"/>
    <dbReference type="HOGENOM" id="CLU_087636_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNL2"/>
    <dbReference type="OMA" id="GITFRIQ"/>
    <dbReference type="OrthoDB" id="167315at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008213">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902073">
        <property type="term" value="P:positive regulation of hypoxia-inducible factor-1alpha signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033235">
        <property type="term" value="P:positive regulation of protein sumoylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="RWD_RWDD3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RWDD3_C"/>
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        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
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        <property type="entry name" value="RWDD3"/>
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        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15628:SF1">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC-like"/>
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    </dbReference>
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        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="263" mass="30438" checksum="313BA985D0ACF5EA" modified="2014-04-16" version="1">MSGTALEEISVLTAIYCEKDEFELLQQSDTKGFIFRIQIALERCSEKLLLKLVFQLPPEYPFCLPDISISSEQLTRKQCINIKHSLLKRASMLISEPMVHELLVWLQQNFTAITTAEQSPDYSSPGRSCGERCEQETWTALLLIDHMRAKTKYTKMIEKWTSELELTGRLFLGKLILILLQGTKENIKEYLLLQKTIKVDVDSSGKKCKEKMMRVLWEAQLPAAHKQLPAFEVKDYSSLEELKKEFELAELLELYQEFVHTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP30</accession>
    <name>W5MP30_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010139"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010151.1">
        <property type="protein sequence ID" value="ENSLOCP00000010139.1"/>
        <property type="gene ID" value="ENSLOCG00000008350.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S4JY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014131"/>
    <dbReference type="HOGENOM" id="CLU_1160783_0_0_1"/>
    <dbReference type="InParanoid" id="W5MP30"/>
    <dbReference type="OMA" id="VTIECTF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008350">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039089">
        <property type="entry name" value="IGSF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15297">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15297:SF2">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866396" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="29"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="21984" checksum="C31EF79E0F03DF12" modified="2014-04-16" version="1">MARVSSNSLDRLCIAGLLLALSIVQEACPADGCSVQVSQPGEMSVVEDTSSVTIPCAVTAPGCPGHRSVYWFVFRASSHEQLQLHTHGKYAPQGDSLDISPVHANDSGVYYCGIAFSGSTAPGARSTGKGTVLLVRGRLHVANPQGKALLSTVIVLLFLYNLAIIILLIHKKNRNVVRFFRFPQNNSTWRNNFRAVVQEMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPS1</accession>
    <name>W5MPS1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zgc:153441</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPS1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010380"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010394.1">
        <property type="protein sequence ID" value="ENSLOCP00000010380.1"/>
        <property type="gene ID" value="ENSLOCG00000008528.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162345"/>
    <dbReference type="InParanoid" id="W5MPS1"/>
    <dbReference type="OMA" id="DPWGWVD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008528">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF54">
        <property type="entry name" value="RETINOL DEHYDROGENASE 12-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="334" mass="36319" checksum="545217316DF3B08A" modified="2014-04-16" version="1">FGACLDMWDQGQWFSLLTRCGAVTVVTAISLVLLRKWISGGVCRSRARLDGKTVLITGANTGIGKETARDLARRGARVVIACRDLAKAEAAAAEIRRTTGNGNVAVRHLDLASLCSVRQFARDYLASEERLDVLINNAGVMMCPKWTTEDGFEMQLGVNHLGHFLLTNQLLGKLKSSAPSRVVNVSSIAHKGGQIHFDDIFFDKTPYSSLVSYRQSKLAVLLFSRELARRMQGSGVTSYSLHPGVIRTELGRHVLTGYPLLKAIVSGPSLLLMKTPAEGAQTSIYCAVTEGLEKYSGCYFSDCALKQPAPEGKDDQAARQLWELSAKLVGPTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPZ7</accession>
    <name>W5MPZ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitogen-activated protein kinase 15</fullName>
            <ecNumber evidence="1">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01020214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPZ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010456"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010471.1">
        <property type="protein sequence ID" value="ENSLOCP00000010456.1"/>
        <property type="gene ID" value="ENSLOCG00000008598.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159758"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_14_1"/>
    <dbReference type="InParanoid" id="W5MPZ7"/>
    <dbReference type="OMA" id="CWRETAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008598">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07852">
        <property type="entry name" value="STKc_MAPK15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000238">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000166">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="581" mass="64846" checksum="580252C0D24AFCD4" modified="2014-04-16" version="1">MNVTAVEDHISLKYEIKRRLGKGAYGIVWKAIDRKTGEIVAVKKIFDAFRNRTDAQRTFREIMFLQEFGEHPNIIKLLNVIRAQNDKDIYLVFEYMETDLHAVIKKGNLLKDIHKRYIMYQLLKATKFLHSGNVIHRDQKPSNILLDTDCFVKLCDFGLARSLYQIQEDAGNPALTEYVATRWYRAPEILLASTRYTKGVDMWSIGCILGEMLLGKALFPGTSTINQIERIMSAIPPPIHEDIYSIKSDYGASVIQRMLSRSRIPLEDLLPPSVPPDALDLLQKLLVFNPDKRLTAEEALAHPYVSRFHSPAKEPSLDYDVVLPVDDDTQLSVAEYRNKLYEMILDRKASLRLQRQQGRLKEAAVGEQQERPPEEQKEASPPAPEATLPPPGAAAQKDRAGTSPDYSPKATKTTYNPITHSPASNPSTAALQRGATAGRKLPRQTSHENVTAPPAASQGPNGLNSQAPVRGAEQHRGRSAPIARARSFSLTQNRFNNPLVRKEEPDVAAGMSVGTARLNQRSHSQTRESKPIPKFSKKTFQNASNVGAAGDPKAKLGSYAQAYGTINKTELENLLRVRSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQD4</accession>
    <name>W5MQD4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the riboflavin transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQD4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010608.1">
        <property type="protein sequence ID" value="ENSLOCP00000010593.1"/>
        <property type="gene ID" value="ENSLOCG00000008703.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003774"/>
    <dbReference type="HOGENOM" id="CLU_034789_1_0_1"/>
    <dbReference type="InParanoid" id="W5MQD4"/>
    <dbReference type="OMA" id="SWVLCMG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008703">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929:SF1">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52, RIBOFLAVIN TRANSPORTER, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="442" mass="47576" checksum="79FBBDC92FE5064B" modified="2014-04-16" version="1">IKRPPSGRLCLVSHVLVALFGMGSWISVNSLWVELPVVVNVLPEGWNLPAYLSILIAFGNVGPVVVTLTHHFAPGRLNERLVIHSIQALAVVASAFLAIFWDEIVLVSGKSHSVPYLLLTFILALVCCTSNVTFLPFMFRYPSVYIRTFFVGQGLSALFPCVVALGQGVGKLECLNGTSGNGTLSPHYLDENFPARNFFWFLFTMLGISGLSFLALSFRIPTQPAQEEKDLGSPSSKEESETHPLQNGTPHVAEDQVDVSKDSPHSAFWTRRNIYLLALLGISNALTNGILPSVQSFACLPYGTMTFHLSVVLGNIANPLACFIAMFVLCRSSAGLGAMSVAGGAFATYLLVLAALSPCPPLLGNPVGVSLVVISWIVFTALFSYLKVVIGSLLHEAGHAALLWCGVFIQAGSLIGALIMFPLVSVYDIFQRAQDCVDNCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSF6</accession>
    <name>W5MSF6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Potassium channel regulator</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639331.1">
        <property type="nucleotide sequence ID" value="XM_006639268.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSF6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011315"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011331.1">
        <property type="protein sequence ID" value="ENSLOCP00000011315.1"/>
        <property type="gene ID" value="ENSLOCG00000009277.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685011"/>
    <dbReference type="KEGG" id="loc:102685011"/>
    <dbReference type="eggNOG" id="KOG2723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161898"/>
    <dbReference type="HOGENOM" id="CLU_087954_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSF6"/>
    <dbReference type="OMA" id="SYIMDFL"/>
    <dbReference type="OrthoDB" id="10025005at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009277">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF5">
        <property type="entry name" value="POTASSIUM CHANNEL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="273" mass="31471" checksum="8403DD9B5437884B" modified="2014-04-16" version="1">MSNQDIITLNIGGQKCTTFVSTLRKHPDTRLARMLDGGDPEFRVINGQVFVDRDGSLFKYILDYMRTHHISLPPDFVDYEGLIREAEFYEVRDLAEILSKDGGRQRMEILEVRFSVQETHSFFRIFCSHCSTVEMLASRISVFAEQPTLTWNYAYQPQKPMVLVPLQRPSHHDLVFHCGTSFSASEEFAARYVTIKPDERKLINGTNILGLLVDLLLKEGFRLISTRTVSTEEKIECFTLERRTTPQILTVRDGYPAEQISHQSKHGKLLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU56</accession>
    <name>W5MU56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal trans-2-enoyl-CoA reductase</fullName>
            <ecNumber evidence="13">1.3.1.38</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in chain elongation of fatty acids. Catalyzes the reduction of trans-2-enoyl-CoAs of varying chain lengths from 6:1 to 16:1, having maximum activity with 10:1 CoA. Has no 2,4-dienoyl-CoA reductase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-decenoyl-CoA + NADPH + H(+) = decanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44960"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:44961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2E)-dodecenoyl-CoA + NADPH + H(+) = dodecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:44965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-hexenoyl-CoA + NADPH + H(+) = hexanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44956"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44957"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E)-octenoyl-CoA + NADPH + H(+) = octanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:44953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2E)-tetradecenoyl-CoA + NADPH + H(+) = tetradecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a (2E)-enoyl-CoA + NADPH + H(+) = a 2,3-saturated acyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33764"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PEX5, probably required to target it into peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.1.38" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01016456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU56"/>
    <dbReference type="FunCoup" id="W5MU56">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011915"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011936.1">
        <property type="protein sequence ID" value="ENSLOCP00000011915.1"/>
        <property type="gene ID" value="ENSLOCG00000009750.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156882"/>
    <dbReference type="InParanoid" id="W5MU56"/>
    <dbReference type="OMA" id="GYRICIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009750">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019166">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033306">
        <property type="term" value="P:phytol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000335">
        <property type="entry name" value="Peroxisomal trans-2-enoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052388">
        <property type="entry name" value="Peroxisomal_t2-enoyl-CoA_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317:SF7">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="301" mass="32107" checksum="D4A5A1A111DB7C76" modified="2014-04-16" version="1">VQDMAASSVFKFGLFNHKVAIVTGGGTGIGKAITSELVHLGCSVVISSRKLEHLEAAAKELTERIPVSSPASVTPIQCNIRSEAEVKNLVASTLSLHGRIDFLVNNGGGQFSSPVELINAKGWNAVIDTNLTGTFYCCREVYNAWMKEHGGAIVNIIADMWKGFPGMAHTGAARAAVDNLTKSLAIEWASSGVRINSVAPGTIISKTAMANYKEIGPQLFKMSVDFCPAKRLGVPEEISPAVCFLLSPAASFISGETIKVDAGQSLYSSMWKVPDHSAWPPAPEGENTDTLREIMKSKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUS3</accession>
    <name>W5MUS3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat domain 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat AIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUS3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012132"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012153.1">
        <property type="protein sequence ID" value="ENSLOCP00000012132.1"/>
        <property type="gene ID" value="ENSLOCG00000009921.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009416"/>
    <dbReference type="HOGENOM" id="CLU_015246_3_0_1"/>
    <dbReference type="InParanoid" id="W5MUS3"/>
    <dbReference type="OMA" id="FYQGPPF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009921">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000097">
        <property type="entry name" value="WD repeat domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000203">
        <property type="entry name" value="WD repeat domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="327"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="539"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="617" mass="68510" checksum="E8D7B47CB2743B79" modified="2014-04-16" version="1">ARMSYELKKVFASLPQVERGVSKILGGDPKGKNFIYTNGKSVTIRNIDNPAIADIYTEHAHQVTVAKYAPSGFYIASGDISGKVRIWDTTQKEHILKYEYQPFSGKIKDIAWTEDSKRLAIVGEGREKFGAVILWDTGSSVGEITGHNKIINSVDIKQTRPYRLVTGSDDNCAAFFEGPPFKFKFTMADHSRFVNCVRFSPDGNRCASAGADGQIFLYDGKTGEKLAALGGGKAHDGGIYAVSVGNCFNFMLKAAADKRYFLWNILENDLGVYFNMDSCLLKRHLDLFWKKLCRCFGIDEIRSPCLMFFYFRRKNNYYKQIRVFIFNKGHSKSIQSLTIHKNNGRSYIYSGSHDGHINYWDAETGENDDFSGKGHSNLVSKMAVDESNHLVTCSMDDTVRFTSVTKKEYSASDIVKMDVQPKCVSVGPGGYALAVCIGQLVLIKDKKKCFTMDNLGYEPEAGAIHPGGTTVAIGGADGKVHFYSIQGNTLKEDGKVIEAKGPVTDMAYSNDGAFLAVSDAQKVITVFTVADGYAEKNAFYGHHAKVVCLAWSPDNERFASGGMDMMVFIWTVNDADKRLKIPDAHRLHHVSGLAWLDEHTLVTTSHDCSVKQWTVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVP8</accession>
    <name>W5MVP8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVP8"/>
    <dbReference type="FunCoup" id="W5MVP8">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012457"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012478.1">
        <property type="protein sequence ID" value="ENSLOCP00000012457.1"/>
        <property type="gene ID" value="ENSLOCG00000010175.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0457">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157318"/>
    <dbReference type="HOGENOM" id="CLU_018273_4_2_1"/>
    <dbReference type="InParanoid" id="W5MVP8"/>
    <dbReference type="OMA" id="KLKFNRT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010175">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000185">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000170">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000011">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056267">
        <property type="entry name" value="Ada2b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF63">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24533">
        <property type="entry name" value="Tri-helical_Ada2b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="360"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="489" mass="54097" checksum="908AD3A1C3BAA439" modified="2014-04-16" version="1">AHAPALEAVQLQQQAAAACNMADLGKKYCVNCLADVTNLRLRCTECQDIELCPECFSAGAEIGNHRRWHGYQQVDGGRFTLWGPEAEGGWTSREEQSLLDAIEQYGFGNWEDMAAHVGASRTPQEVMEHYVTMYIHGNLGKACIPDSIPNRVTDHTCPSGGPLSPSLTTPLPPLDITVAEQQQLGYMPLRDDYEIEYDQDAETLISGLSVNYDDDDVEIEMKRAHVDMYVRKLRERQRRKTIARDYNLVPAFLGKDKKEKDKEKPAAGGAATAAAVVAAAAPPAAAGSSAATPAPKRKITKEEKEQRLKLRPLCQFMACREFEEFFENMHKERVLRAKVRELQRYRRNGITRLDESVEYEAARHKREKRKENKSVAQSKRGGGGGGGSGGGGGGGGGGGKEEGKDSEFAAIENLSGFELLSDREKVLCNSLNLSPTRYLTAKTIIIKDHLQKRQGIPSKSRLPSYLDKVLKKRILNFLTESGWISRDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX78</accession>
    <name>W5MX78_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gfo/Idh/MocA-like oxidoreductase domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX78"/>
    <dbReference type="FunCoup" id="W5MX78">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012987"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013015.1">
        <property type="protein sequence ID" value="ENSLOCP00000012987.1"/>
        <property type="gene ID" value="ENSLOCG00000010603.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160837"/>
    <dbReference type="InParanoid" id="W5MX78"/>
    <dbReference type="OMA" id="NQKDYVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010603">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050463">
        <property type="entry name" value="Gfo/Idh/MocA_oxidrdct_glycsds"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43818">
        <property type="entry name" value="BCDNA.GH03377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43818:SF2">
        <property type="entry name" value="GLUCOSE-FRUCTOSE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="129"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="43401" checksum="CD57C93929E59F85" modified="2014-04-16" version="1">MLPGVGVFGTSLTARVIIPLLKNEGFAVKALWGRTQEEAEELAKEMNVPFYTNRIDDVLLHQDVDLVCINLPPPLTRQIAVKTLVCIGKNVICDRTATPLDAFRMMSAAHYYPKLMSIMGNVLRFLPAFVKMKELLEEGYVGELLVCEAQVHGGSLLGKKYNWSCDDLMGGGGLHSVGSYIIDLLTFLTGQKAAKVHGFLKTFVKQTDHIKGIRQITSDDFCTFQMVLERGACCTVTLNFNVPGEFRQEVIVVGSVGRLTVSGTDLYGQKNDAPNRELLLKDCTPMGNASLPEKAFSDIPSPYLTGTIRMVQAVRQAFEDQDDQRTWDGRPLTMAATFEDCLYALCVVDTIKKSSQSGEWQNIVVMTEEPELSPAYLISEAMRRSRMSLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXF4</accession>
    <name>W5MXF4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXF4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013063"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013091.1">
        <property type="protein sequence ID" value="ENSLOCP00000013063.1"/>
        <property type="gene ID" value="ENSLOCG00000010663.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167569"/>
    <dbReference type="InParanoid" id="W5MXF4"/>
    <dbReference type="OMA" id="KIPWRYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010663">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF51">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="4">
        <location>
            <begin position="116"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="484" mass="54210" checksum="EA5F3C8766FB10B6" modified="2014-04-16" version="1">MESNLSSRGARIAAYRGVHRTGFDMYIKDPFNKENNPQGILNFGNSENKLCYDILEERIQLEYCLSKASDSLAFRGVVARGSSRGSPRDHGEEERMNSTGPAQATSKLSRAQANCLTPELLQYSDLQGLRGFREETADFLTEYCRAPTPLNPEHIVVLNGCTSALAALTTVLMEPGDGYLIPSPYYGGIATYTWLYGEVNPVPVPLDSKISRGKGYPLMLAVDKLEDAMSEATKKGIRVRGLILTNPHNPLGVVYPAEVLMDCLEFAHRYKLHVIVDEIYMLSVYDGKAFTSIHSFESLPDPERTHVLWGFSKDFALAGLRVGTVHTRNQEVRRALIRLASFHSCAGPTQQLLTHLLQDRDWINTTFLPSNRRRLLESRAILVKGLQDIGVSVLKSSAGFFVWADLRSFLKEQSFEAEMDLWWRLIHEKVFISPGQVFSSCEPGWFRVVFSDTVENIKIGVDRIKKALGAPAIIAEKYVHHHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXN6</accession>
    <name>W5MXN6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Adenosine receptor A1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14 15">Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXN6"/>
    <dbReference type="FunCoup" id="W5MXN6">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013145"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013173.1">
        <property type="protein sequence ID" value="ENSLOCP00000013145.1"/>
        <property type="gene ID" value="ENSLOCG00000010733.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234555"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_5_1"/>
    <dbReference type="InParanoid" id="W5MXN6"/>
    <dbReference type="OMA" id="FCCKDTP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010733">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15071">
        <property type="entry name" value="7tmA_Adenosine_R_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000061">
        <property type="entry name" value="Adenosine receptor A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001068">
        <property type="entry name" value="Adeno_A1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF1">
        <property type="entry name" value="ADENOSINE RECEPTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00552">
        <property type="entry name" value="ADENOSINEA1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11 15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023139"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="347" mass="39280" checksum="BD9C11E916009AFD" modified="2014-04-16" version="1">VRKFFALEFNFTVLRMSGQLGPSQVLYIGMEVVIAVSSVLGNVMVVWAVKINRSLRDTTFCFIVSLALADIAVGALVIPLAITISIGLQTHFYSCLLVACTVLVLTQSSILALLAIAIDRYLRVKIPTSYKRVVTKKRAGAAVVACWTVSFIVGLTPMLGWNNLQKMKENGTQITSDLTVTCQFENVISMEYMVYFNFFGWVLPPLLLMVLIYVEIFYMIHKHLNKKVTANSSDPSKYYGKELKLAKSLALVLFLFAISWLPLHILNCITLFCPSCQKPMILIYIAILLTHGNSAVNPIVYAFRIKKFRSTFLKIWNHYFCCKERKRPTNFQSERSDFQGRVPQENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY29</accession>
    <name>W5MY29_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000010845.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG14"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="303" mass="34475" checksum="AD79F67E61E67D07" modified="2014-04-16" version="1">RQLSTQNQIKHLRVPGLQEHYTALFIFFLILFLATFIGNLLIVVLVALDHRLHMPMYFFLWNLAFLDILTTVSLIPKMLAVFLTIDRTISLTGCFAQMYFIISVGATEEFLVAVMAYDRYIAVVKPLHYNNIINTKVCLTITATVWVIGFLVPLVSVVLASHLPFCGSNYILHCFCDYPTVMSLACGDITVQVNFALLLAMIVIYVTFLFVLWSYCRILWSVIKMKTVESRKKAFSMCSSHMMAVFLYYVSTAVVYSGLRVESISPDGRIFIGGLNYFLVPLVNPIIYSLRNEKIKAAAQRHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N077</accession>
    <name>W5N077_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transmembrane protein 150B</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Modulator of macroautophagy that causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Represses cell death and promotes long-term clonogenic survival of cells grown in the absence of glucose in a macroautophagy-independent manner. May have some role in extracellular matrix engulfment or growth factor receptor recycling, both of which can modulate cell survival.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DRAM/TMEM150 family.</text>
    </comment>
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    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000014065.1">
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        <property type="gene ID" value="ENSLOCG00000011419.1"/>
    </dbReference>
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    <dbReference type="CTD" id="284417"/>
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        <property type="component" value="Linkage group LG12"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:autophagosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CWH43_N"/>
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        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF3">
        <property type="entry name" value="MODULATOR OF MACROAUTOPHAGY TMEM150B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
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        <location>
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            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="15">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="233" mass="25372" checksum="2BF11C2C4C258CD6" modified="2014-04-16" version="1">MWAWALLPIAMAMLGTVGFWAVFGMAVSNGSVNLTREFPYISTCGTYNPQSCIFAQVLNLGSLMVVWIVLIRYQQIKDYGQSSRVNVAGLVLGFGCALGCSFVGNFQQSVLMGAHLFGAFLAFFVGLAYFWVQAYLTYTVAPRHGGRWIGPARVGLCSACTVLIVSMSVLHKQQYRSAAAGCEWAAAMTFFLLFALFAVEFRHVDCHRYHVQKRAVPAGGAMPAEVASQAQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0C8</accession>
    <name>W5N0C8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MRN complex interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0C8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014087"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014116.1">
        <property type="protein sequence ID" value="ENSLOCP00000014087.1"/>
        <property type="gene ID" value="ENSLOCG00000011470.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107077718"/>
    <dbReference type="KEGG" id="loc:107077718"/>
    <dbReference type="CTD" id="51149"/>
    <dbReference type="eggNOG" id="ENOG502S8YD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006124"/>
    <dbReference type="HOGENOM" id="CLU_672597_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0C8"/>
    <dbReference type="OrthoDB" id="5960226at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011470">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032739">
        <property type="entry name" value="MRNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049472">
        <property type="entry name" value="MRNIP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15863">
        <property type="entry name" value="MRN COMPLEX-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15863:SF2">
        <property type="entry name" value="MRN COMPLEX-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15749">
        <property type="entry name" value="MRNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MRN complex-interacting protein N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="409" mass="46827" checksum="E14845159551DAF6" modified="2014-04-16" version="1">MVQEFQVLRCFTCKTFQVHQVKKSNKWNCKMCGEKQSLMKIYGQGSGADCRRHVQKLNAMRGEIIQAEEDHACEQARFSEDIQSQDENLSIAVEPNVEAEKEASCWSNYLQDSTEESREETTRSEEDNLYTDSSCFNVQNVINKTSRKRKKSWDSANTPTKEFTNHVFYEGVWKKCSDQEKNEKPMMDERRSLTLINRNSKLLESSTTGSPGASAAKRVSTAIKASSNPGYNAKHSELKALKWDKYLSFPSEDKKEKRDFQGQREREITVTTDARLEGDIFSIVKSIADDSYEDRRCYKVQEVHCENKTHQISTVQNQCLFPETVPETVTSSIMYQNATEIRTLSAKTGDWHSNFELNLESSDCASESQLCQDPLFPSSTINGKINPARKQYSSMSSLFQTDEDFDDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N137</accession>
    <name>W5N137_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 57</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632532.1">
        <property type="nucleotide sequence ID" value="XM_006632469.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069049334.1">
        <property type="nucleotide sequence ID" value="XM_069193233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N137"/>
    <dbReference type="FunCoup" id="W5N137">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014346"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014375.1">
        <property type="protein sequence ID" value="ENSLOCP00000014346.1"/>
        <property type="gene ID" value="ENSLOCG00000011675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694747"/>
    <dbReference type="KEGG" id="loc:102694747"/>
    <dbReference type="CTD" id="255252"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157393"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_15_1"/>
    <dbReference type="InParanoid" id="W5N137"/>
    <dbReference type="OMA" id="AYMERYT"/>
    <dbReference type="OrthoDB" id="1728874at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011675">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000458">
        <property type="entry name" value="Leucine rich repeat containing 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000230">
        <property type="entry name" value="Leucine-rich repeat-containing protein 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF62">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="26390" checksum="1CC445F8A1A30EB1" modified="2014-04-16" version="1">MGNSALKAHIETSQKTGVFQLTGKGLPEFPEDLQRLSSNLRTVDLSNNKIDVLPSFIGTFLSLKSLTLNCNKLTSLPEEIGKLKKLETLHLNGNQLQRLPVSVGQLTALRTLGLSGNRLREFPKNLGSLRHLDLLDLSKNHIQAVPAEVGTLQAIEINLNQNQISEVSPDVSKCPRLKVLRLEENCLELSSVPLSIFSDSQVSLLALEGNLFEVKKVRDLGGYDKYMERFTATKKKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N272</accession>
    <name>W5N272_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Contactin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N272"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014731"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014760.1">
        <property type="protein sequence ID" value="ENSLOCP00000014731.1"/>
        <property type="gene ID" value="ENSLOCG00000011962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3513">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164703"/>
    <dbReference type="InParanoid" id="W5N272"/>
    <dbReference type="OMA" id="MPDANPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011962">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd04969">
        <property type="entry name" value="Ig5_Contactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000054">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000600">
        <property type="entry name" value="Contactin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000273">
        <property type="entry name" value="contactin-3 isoform X1"/>
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        <property type="match status" value="10"/>
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    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
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        <source>
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    <sequence length="1047" mass="115130" checksum="5C40ECBC9E15E76A" modified="2014-04-16" version="1">TAFFLFVACKAVGVWLLWFPVGVCVPCVSGIGVLGSSYGPVFEEQPSSLLFPDGLTDEKITLACRARASPPATYSWKVNGSDVQVGSEPRYALVAGNLVISSPQRGKDSGSYQCLATNPSGTIISREASLRFGYLHEFPPDDRSLQTVYEGAGTMIPCHPPSHYPALSYRWFFNEFPTFIQPDRGRWFVSQATGNLYLSRAEPNDTGNYFCFATNNMELSIKSVFSKPNQLNVLPDATPRKSSPNIKVRFPSDTYALAGQSLQLECFAYGNPVPKLRWRKVDGTVPPKALGSMDGPILHLPELDFMDEGVYECEARNSEGRDTYQGRITVQAQPEWLQVMSDSEVEISSELRWSCAAAGKPRPSIRWLRNGQPLASQDRIEVNAGQLRFSNLSLEDSGMYQCVAENKHGTIYANAELKVAVQAPDFRLSPVRRLTPSARGGQVKMECRPRAAPRPVLFWSRGTEILSNNSRVTVTPDGSLWIQNISRSDEGKYTCFAENFLGKANSTGFLSVREATKITLVPSNADINQGENVTLQCHASHDPTMDLTFTWSMNGILLDTEDASRHFHRVEGKETVGDLMIVSGQLGHAGIYKCTAQTVVDSASASARLVVRGPPGPPGGVVVKDMNETSVELRWSRGYDNHSPIGKYTIEGRSPLSEAWKQMRTDPANIEGNAESAHVIGLMPWMDYEFRVIASNILGSGEPSMASQKVRTRPAAPMVAPSGLGGGGGDRHELIITWTSMAREYQNGEKFGYILAFRKKAAPQWHIVKVPNVESSRFVYHNESLAAYCPFEVKIKAYNEKGEGPFSQTALVYSAEEEPTETPRRVNATSLSAFEIQVSWEPLQHLSTNGILRGYEIRYWRQNEKEAAADRVRTMGLETVARVSGLRPSTLYHVTVLAYNSAGTGPPSPFTNVTTKKPPPSRAPDNVSWRIDGSWVTVAWDHVKPLKNESAVLGYKVLYKHEGQTALKVLEKGKASVRLPLPQDNGYVVLEIRAWGEGGDGAPHELIVSRDSGTGMMVQNAVKDLTPLHTVVTATAVTLTLIGLLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2W2</accession>
    <name>W5N2W2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylinositol glycan anchor biosynthesis class G (EMM blood group)</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2W2"/>
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        <property type="interactions" value="748"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014971"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015000.1">
        <property type="protein sequence ID" value="ENSLOCP00000014971.1"/>
        <property type="gene ID" value="ENSLOCG00000012169.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144269"/>
    <dbReference type="HOGENOM" id="CLU_004770_4_0_1"/>
    <dbReference type="InParanoid" id="W5N2W2"/>
    <dbReference type="OMA" id="RVKFGHD"/>
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        <property type="component" value="Linkage group LG4"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:GPI anchor biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Putative GPI ethanolamine phosphate transferase 2"/>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
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        <property type="entry name" value="Phosphodiest/P_Trfase"/>
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        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
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        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="710"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="747"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="875"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="912"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="948"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="12">
        <location>
            <begin position="642"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="976" mass="109194" checksum="733166D5FD4D34AA" modified="2014-04-16" version="1">MKIRSSVFASLCLVIEVFGIVLFLRGFFPVPVKSSLSSKSKVSDLPLEPLEGHAANSSRIPPPLFKRVVIMLVDALREDFVFGPKGKEFMPYTRHLVERGSSHSFVAKARAPTVTMPRIKALTTGSIPGFIDVVMNLNSPALQEDNLIWQAKTAGKKVVFYGDDTWIRLFPKHFMEYDGTTSFFVSDYTEVDNNVTRHLDSTLKRDDWDVLILHYLGLDHIGHLSGPHSSLIGPKLLEMDDILKKIHTALISKVKHGLLKNLLKMCVEHSRSNIPLCSGSSQLKLSGHPFALLVLIASYFKTVRIGFETPEPVEQVDFTPTLALGLGLPISQNNVGHLILPVFEENTLREQLRFLHLNGFQLSHLIKDSTPDFEKDPGYEQFRIAEKSHGSWMKLYLEGNTSEVLSNMGKKVLKQYMDALKSMSESLSKQLGKYDMYSMIVGMSIVLEVLFLLLITMAEALSGAAEVDLPLSSALCSLPFYLLCLFCSSIHVMVCTSTESTCYFCSLSWALVFAVIAFFSSLLCVILSIIGRKLFWAKPQMKNILKAQSSDSGFSELDILLLTGTLGHVLSLGASSFIEEEHQTWYFLLNTLCLAVFQDVCRKYFREKKSHRDLDENSYLPSKDDTPTVDYSSRTSALAGYEKWLALVTPLATLVCCRMLRSLNQTGVQWAHLPDLGHWLNNSEHIVELSLLAAASLVLIFLLVQRRCSLVSKIALGLGLLGVYSYRAAVGNVLFPWQQDGRDISKGTVEARFVYIFVLGILFTGAKDLLRSQVISSDTQLKSRGLWEIYSGVVLLAALLFRPHNLPVLLFCLLIQTIMAQFIWKELQYDAAQTTIMHYWFGQAFLYFQGNSNNIATVDISAGFVGLENYVEIPAIFLTAFCTYAGPLLWACHLVCYLSSERERSTAAIGHGCYCFALLRSIPAAAYIILVTALRYHLFIWSVFSPKLLYEGMHTLVTAAVCVFFTAMDQNHAVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N551</accession>
    <name>W5N551_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Cholinergic receptor, nicotinic, alpha 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N551"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015760"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015790.1">
        <property type="protein sequence ID" value="ENSLOCP00000015760.1"/>
        <property type="gene ID" value="ENSLOCG00000012810.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158062"/>
    <dbReference type="HOGENOM" id="CLU_013592_0_0_1"/>
    <dbReference type="InParanoid" id="W5N551"/>
    <dbReference type="OMA" id="VEWNISS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012810">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0095500">
        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051899">
        <property type="term" value="P:membrane depolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099171">
        <property type="term" value="P:presynaptic modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035094">
        <property type="term" value="P:response to nicotine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic alpha 6 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000051">
        <property type="entry name" value="Cholinergic receptor, nicotinic, alpha 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000022">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit alpha4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="640"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="674"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="841"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="165"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="418"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="615"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="865" mass="100083" checksum="E30FF3AD10D82DDE" modified="2014-04-16" version="1">MTTNVWLTQEWNDYRLKWDPQEYEGIKKLRIPSQHIWLPDIVLYNNADGTYEVSFYCNAVVSSSGDVFWLPPAIYKSACKIEVKNFPFDQQNCTMKFRSWTYDRTEIDLILMSDFASRDDFTPSGEWDIVSLPGRKNEDPEDATYVDITYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLAVPLIGKYLMFTMVLVTFSIVTSVCVLNIHHRSPSTHTMPEWVKRVFLDKLPAVLFMKRPDNTNVRERFRRKQQNSHPDLKAGKGDADSFYVNEESAKKYGWKISDLPENTEFRKRMTVKCNPDMEEAVDGVRFIAEHMRSEDDDESIIEDWKYVAMVIDRLFLWIFVFVCVVGTVVWSIGGCTTEGESAKKLQLGSNQWIRENNKHKHKHKCVLQRRKIKVLCTDEVNQIMETNLWLRHIWNDYKLKWIPAEFDGIEYIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKYDGTVTWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDLVLIGSKVNLKDFWESGEWEIIDAPGYKHDIKYNCCEEIYPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPMTHTMPVWVRSIFLGVLPRIMFMRRPLDPQTPKGGSKKRKNSASQQGAMNCLEYGENKLPKDQKKCLCQHPKEPSEPDTSKLSRQLSPQSINTVLAFSVVSPEIKQAIESVKYIAENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFVLVCVLGTLGLFLQPLIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5R0</accession>
    <name>W5N5R0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Centrosomal protein 131</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5R0"/>
    <dbReference type="FunCoup" id="W5N5R0">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015999.1">
        <property type="protein sequence ID" value="ENSLOCP00000015969.1"/>
        <property type="gene ID" value="ENSLOCG00000012955.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT0Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001758"/>
    <dbReference type="HOGENOM" id="CLU_010690_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5R0"/>
    <dbReference type="OMA" id="RKECTLA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012955">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010824">
        <property type="term" value="P:regulation of centrosome duplication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030465">
        <property type="entry name" value="CEP131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31540">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 131 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31540:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 131 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="641"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="702"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="787"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1161" mass="133037" checksum="08BA65A6E6F2A787" modified="2014-04-16" version="1">MHTARSPPSLPGNASVGAGDALDLSLTGSQLSVSRRPSSAFQAKHFSRSVSLACDGKGKRNALTEAGLASTRSIKNLRRSNSTTQVNQRPNTSVSEDQSDDFLALFDSSSDGRRKLASLSKASPDRTTWNILDDQPRAFPTPGSARSTSTVDSPVGMKKREAGIALAANFTANNRSNKAAVGNSVTTILHNNHSDKTFTPKSSNQKPSLNNIIKATVHDEGSLENGSLTKSQKNFSTNNNMALGSPTHLRRQEVTEEEAERFIQQVNHAAVTIQRWYRRHVQRQRAGEAALKRLLATKKREREQKAEEGKTQEKKKEEDRKQIRQEKARLARRAAIQELQQKRAQRAAEAQRVAEEELEAMRQSGKVGRKKPTKTSPSNRSSSSASDIKTKNTGEGRRVPVHKPCCSQAMVKPNNLYTGILILKGTNTICLQDPSYKSFEFNVILTLPSFLLNSAPISLTDANVNVPAELDDISNLRAGSPTDSPGRGSQCSQELLHKRMSQEEPQQGAPSSIAQSKTTLNDLLDTLKLLEEEPEKLPEPKSYRKDKYSWIDEDVDNSLTLDNLERHGKLSQPAALPDGGALLSEAKLQSIMSFLDEMEKSEQERPRSVTSGSHREVLLSEEELAGVEQASATAAEVTGSMMRLKLELEEKKRTVNMLQTALAQQRELTVRHTKETEKELNRSFQLQKEQYEATIQRHLGFIDQLIDDKKALSERCEGVINELKQVDQKYTKKIAQMQEQHDLEIKKLKELMSATEKIRREKWIDEKTKKIKEITVKGLEPEIQKLIAKHKQELKKLRAVHEAELLQADERAAQRYVRQSEELRQQLEKERDEQCQRERELAKQRYEKQLQEEEQSLQQQRRRLYSEVAEEKERLAQLASRQRAELEELRRQLEENSSLAGRALREEFDKARDEQERRHQAEMKALKERLEIEKQAWEENYMKKEEAWLLSRERELKEEVRRGRDKEIELAIQRLEEETSGAREECERTAENRVKRAREKYEAELRELERSERSLQDRNNELKKRLGELEGEVIRLQGLLQQKEQEVQDITQIRDKLAEERRSLAEVIRQEFADRLVATEEENRRMKTEVSETRARHRLEVERVTREKEEELAEVHQRVKAAILKKEETVNNLRKQYEAALKRADHLEALLEQQRKQLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6G7</accession>
    <name>W5N6G7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-glutamate cyclase</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6G7"/>
    <dbReference type="FunCoup" id="W5N6G7">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016226"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016256.1">
        <property type="protein sequence ID" value="ENSLOCP00000016226.1"/>
        <property type="gene ID" value="ENSLOCG00000013170.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002237"/>
    <dbReference type="InParanoid" id="W5N6G7"/>
    <dbReference type="OMA" id="MDSSHEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013170">
        <property type="expression patterns" value="Expressed in mesonephros and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2040.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1640.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2040.10">
        <property type="entry name" value="PSTPO5379-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.20">
        <property type="entry name" value="TON_0340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025504">
        <property type="entry name" value="GLUCM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14336">
        <property type="entry name" value="GLUCM-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate cyclase-like C-terminal" evidence="3">
        <location>
            <begin position="304"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="626" mass="68077" checksum="40134BDF57EE35E3" modified="2014-04-16" version="1">ATMLCAKSLQHSHPAAIRALIRSKNHGIKNTSGIAAGYKQANIVILHKTLADDFEAFSHANNGPLPLLYRSPPGEWTCPKLAEDSDIRTDCPEYCMFENGNFAGSIPSLMSHSEEFEDMVTFYLGCSFSFEQTLRQAGIPVRNVEQDRNVSMYKTSVPCCGVGRFQCPLVVTMRPVPEGMLDAATQATHPASDVHGAPVHIGYPGLIGVEDLSRPDYGDAVELQPGDVPVFWACGVTGVEAVRSCKSPLAFTHSPGCMFLSDCEERASAPRPSAADTPLAFRVAREPLHYSLVSASAVQRIRALETLIRTEPGQRGNRALFLPNELLKACLSLSHANSVLITTGFPTLYNRNPPEETDGLPGALAMATMLQVLNKQVVIVTDNRAFDFSKRIIHDAVDQGVLNSEVPVRSLQATSPTSVFGFLCQEGETSAPRYDHLVAIERASMAADGNYNNARKVNIQHLVDPIDTLFTTARSIPGITTTGMGRGRNEMPVWKHSGEVVSAVPTQKFTGKDESSCFPLFPGVSNWGGYAVACALYILSCCPVHDRYRRKAVGFPQHTQHSSWASALPSVRKEEKLLEILEQYDVRSGAAGNLGLEVDGLRFHDAHSTMIQQLVDIALQPTASLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6H5</accession>
    <name>W5N6H5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rho GTPase-activating protein 44</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Rho-type GTPase-activating protein RICH2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">RhoGAP interacting with CIP4 homologs protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity. Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation. In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with BST2 (via cytoplasmic domain). Interacts (probably via PDZ-binding motif) with SHANK3 (via PDZ domain); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Presynapse</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
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        <location>
            <begin position="7"/>
            <end position="247"/>
        </location>
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            <begin position="474"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="927" mass="100790" checksum="02F0D0018025208A" modified="2014-04-16" version="1">MRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSHSTHKKLTACLQGQQGVDVDKRSVRSPSKKLPLTTLAQCLVEGATVLGDDSLLGKMLKLCGETEDRLAQELILFEFQIERDVIEPLFVLAEVEIPNIQKQRKHLAKLVLDMDSARTRWQQSSKSSGLSSNLQPTGAKADALREEMEEAANKVEICRDQLSADMYSFVAKEIDYANYFQTLIEVQADYHRKSLALLQSVLPQIKAHQEAWVEKPSYGKSLEEHLAISSREIAFPIEACVTMLLECGMQEEGLFRVAPSASKLKKLKASLDCGVLDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQASNIQDQDKRLQALLSACEKLPKANSNNFRYLIKFLAKLTEYQDANKMTPGNIAIVLGPNLLWAQTEGNITEMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNVTGNYGSPVHTNHNANYSSMPSPDMDHSDRKQHDQARRPLSVATDNMMLEFYKKDGMGVRVMDTSWVSRRGSSLVRKTSSTPPSVQPPAPPAELPSLAQSLVPEQPYELSAAPCGGPPAGGAGDRASTDDVSPNWSDSCYAYPSPEDDRPPPPYPTCCPPPHHHYPRAQPSPRPAAPSPDSVTPASFSSSSSCSPHKWTGYSPLPPPPPSASPQQLDINSNPKPSFLHLPKQSSLADALHANPETNMSPLYIKTPLVLTKHDPALSLPPNLPSSAFPQWGSCACSRDRGTRLTRFLDLKTAVPFLKNKSVSSPGKLSGRKYLRDASGPGLTLLLPLSLSLPAVSKKLAPIPPKVPYGQSGGMSDQSTGQPSPVSLSPTPPSTPSPYGLGYSQGYPGLSSSGQVSPAAAPSLSSPPSLSGTLTKSRPTPKPRQRPSLPPPQPPVTPGLSGSSPQPLDHSLLDSMSPGESMSTGDFYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6J3</accession>
    <name>W5N6J3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6J3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016252"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016282.1">
        <property type="protein sequence ID" value="ENSLOCP00000016252.1"/>
        <property type="gene ID" value="ENSLOCG00000013191.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011749"/>
    <dbReference type="HOGENOM" id="CLU_086770_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6J3"/>
    <dbReference type="OMA" id="AFKRRAM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013191">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07977">
        <property type="entry name" value="TFIIE_beta_winged_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000177">
        <property type="entry name" value="Transcription initiation factor IIE subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016398">
        <property type="entry name" value="TFIIE-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="9">
        <location>
            <begin position="55"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="17"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="245"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="293" mass="33173" checksum="9A3115423A665E0B" modified="2014-04-16" version="1">MDPALLRETELFKKRALSTPAVEKRPAFSDSSSSKKKKAKYDQAGSSGSKHGTDSSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTYHLTLDEILDETKLLDIGLKQKQWLMTEALASNPKIEVRDGKYGFKPKYNLKDKKALLRLLDKHDQLGLGGVLLDDVEEGLPNAQKAIKALGDQVVFVTRPDKKKILFYNDKNFQFVVDEEFQKLWRSIPVDSMDEEKIEEYLKRQGISSMQETGPKKLAPIQKRKKPVSQKKRRFKTHNDHLAGVLEDYSEGVPAKKNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7K6</accession>
    <name>W5N7K6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pygopus family PHD finger 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in signal transduction through the Wnt pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628840.1">
        <property type="nucleotide sequence ID" value="XM_006628777.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7K6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016615"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016645.1">
        <property type="protein sequence ID" value="ENSLOCP00000016615.1"/>
        <property type="gene ID" value="ENSLOCG00000013476.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682362"/>
    <dbReference type="KEGG" id="loc:102682362"/>
    <dbReference type="CTD" id="26108"/>
    <dbReference type="eggNOG" id="ENOG502QTIZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063948"/>
    <dbReference type="HOGENOM" id="CLU_686883_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7K6"/>
    <dbReference type="OMA" id="CTHEVND"/>
    <dbReference type="OrthoDB" id="270215at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013476">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15635">
        <property type="entry name" value="PHD_PYGO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000107">
        <property type="entry name" value="pygopus homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052475">
        <property type="entry name" value="Wnt_Signal_Transd_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23194">
        <property type="entry name" value="PYGOPUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23194:SF3">
        <property type="entry name" value="PYGOPUS HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="352"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="176"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="431" mass="46312" checksum="90828BEAD2E52473" modified="2014-04-16" version="1">MSGEQEKDTISLKRNRGGDGGLDGLGGPGGPGTLLGSPDKKKRKSNTQASSFAPLSEYAPPPNPSAEHLVAANPFDDNYNTPSYKPLPSGNPYFGHPHYPGFGGYGPHRMPPHMSPRMPSAYGGPYPMRNQLHPFAQNQMGMGFNRPPGFNYGHHESPGYGNHPVFNNNMPLPPNQPFRPGPGDHFSQMPPHSMNQNSDMGPNFGQEGNSNINPPMAPNMDASPGFMQPQNNFNQTSAPMPKQDFSEATSKSVSQNPAPCKQSQNPEDNVCQDNSADLKTISRSAPANQDGSHSNTLDKLNGMHPNNDAQKNPTQPCGAVEQAPPKRSSSGSSSNKAAVLLNRPGHSSTDPVYPCGICMNEVNDDQEAILCEASCQKWFHRVCTGMTETAYNLLTAEVSAVWGCDTCMEGKGVPLMRTRELVGQQSVNAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7Z3</accession>
    <name>W5N7Z3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Aquaporin 9b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>glycerol(in) = glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:29675"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>urea(in) = urea(out)</text>
            <dbReference type="Rhea" id="RHEA:32799"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198745.1">
        <property type="nucleotide sequence ID" value="XM_015343259.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7Z3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016752"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016782.1">
        <property type="protein sequence ID" value="ENSLOCP00000016752.1"/>
        <property type="gene ID" value="ENSLOCG00000013583.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689516"/>
    <dbReference type="CTD" id="570191"/>
    <dbReference type="eggNOG" id="KOG0224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164919"/>
    <dbReference type="HOGENOM" id="CLU_020019_9_1_1"/>
    <dbReference type="InParanoid" id="W5N7Z3"/>
    <dbReference type="OMA" id="WGFAVLT"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013583">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000005">
        <property type="entry name" value="Aquaporin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF29">
        <property type="entry name" value="AQUAPORIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02019">
        <property type="entry name" value="AQUAPORIN7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="33817" checksum="F7EE3FCB09AE1BD6" modified="2014-04-16" version="1">MERKTRRNLRERFALKNSIVKEALAEFLGTFVLILFGCGSVAQTVLSRGAVGEMLTIHIGFTLGVTMAVYVAGGVSGAHVNPAVSLAMLVLGKLKLVKFPVYVLAQFLGAFAGAAAVYGLYYDAFMDYTNGILIVTGPNATAHIFASYPGRHLSILNGFIDQVIGTGALVLCILAIVDGKNNGAPKGMEPLVIGLIIMAIGVSMGLNCGYPINPARDLGPRLFTAVAGWGRDVFSAGRNWWWIPVSGPLVGGVVGAVLYLLFIELHHAEPQKHLEEENSVKEKKCRKITVDGGLKCKTFEELSRSFDFRSLNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N873</accession>
    <name>W5N873_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur clusters transporter ABCB7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-binding cassette sub-family B member 7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(glutathione)4[2Fe(III)-2S] cluster(in) + ATP + H2O = (glutathione)4[2Fe(III)-2S] cluster(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167627"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67029"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N873"/>
    <dbReference type="FunCoup" id="W5N873">
        <property type="interactions" value="826"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016832"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016862.1">
        <property type="protein sequence ID" value="ENSLOCP00000016832.1"/>
        <property type="gene ID" value="ENSLOCG00000013647.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689186"/>
    <dbReference type="KEGG" id="loc:102689186"/>
    <dbReference type="CTD" id="22"/>
    <dbReference type="eggNOG" id="KOG0057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156281"/>
    <dbReference type="InParanoid" id="W5N873"/>
    <dbReference type="OMA" id="VFHIIPI"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013647">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990748">
        <property type="term" value="P:cellular detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="137"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="468"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="748" mass="82954" checksum="ECBEFB05684FC61E" modified="2014-04-16" version="1">MALLVGSLKCGFYFQRRKLALLLQQTSSYHVLKHHNGQKNRTYHLQQKTCQVSSTCTRPAAWQNIGRDSHRQLLEATKGLKWWDVADKRTCWHGHAGGGLNADPKNVLKEVDARKILAAMFSYVWPKDRPDLRARVAVSLGLLAGAKMTNIVVPFMFKYAVDNLNQMSGNMLNLSDAPSTVATMATTVLIGYGVSRACAALFNEMRNAVFGKVAQSSIRRIAKNVFLHLHNLDLGFHLSRQTGALSKAIDRGTRGISFVLSALVFNLGPTVFEVALVSGILYYKCGGQFALVTLGTLSAYTAFTIAVTQWRTRFRIEMNKADNEAGNAAIDSLLNYETVKYFNNEKYEAERYDGFLKVYESSSLKTTSTLAMLNFGQNAIFSIGLTAIMILASQGVMTGALTVGDLVMVNGLLFQLSLPLNFLGTVYRETRQALIDMNTLFTLLSVDTKIKEKDMAPPLLISPQTATIRFEDVYFEYLEGQKVLNGVSFEVPAGKKVALVGGSGSGKSTIVRLMFRFYEPQKGSIYIANQNIRDISLESLRKAIGVVPQDAVLFHNTILYNLQYGNIHASLEDVYRVAKLAGIHDAILRMPHGYNTQVGERGLKLSGGEKQRVAIARAILKNPPILLYDEATSSLDSITEENILTSMKDMVKDRTSVFIAHRLSTTVDSDEIIVLNQGRVVERGSHQSLLSNPNSLYSELWHTQNSKVLNGNSLPSEEQKRLSRKEEERKKLQEEILNSVKGCGNCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9B2</accession>
    <name>W5N9B2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase makorin-2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase makorin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01015114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9B2"/>
    <dbReference type="FunCoup" id="W5N9B2">
        <property type="interactions" value="952"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000017221.1"/>
        <property type="gene ID" value="ENSLOCG00000013970.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013970">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein polyubiquitination"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="term" value="P:retina layer formation"/>
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        <property type="entry name" value="RING-HC_MKRN2"/>
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        <property type="entry name" value="Probable E3 ubiquitin-protein ligase makorin-1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Pneumovirus matrix protein 2 (M2), zinc-binding domain"/>
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        <property type="entry name" value="MKRN-like"/>
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        <property type="entry name" value="Znf_C3HC4_RING-type"/>
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        <property type="entry name" value="Znf_CCCH"/>
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        <property type="entry name" value="Znf_CCCH_sf"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MAKORIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAKORIN-RELATED"/>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="CCCH zinc finger"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
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            <begin position="234"/>
            <end position="288"/>
        </location>
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        </location>
    </feature>
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        <location>
            <begin position="2"/>
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        <location>
            <begin position="31"/>
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    <feature type="zinc finger region" description="C3H1-type" evidence="17">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="17">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00030863"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
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            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="417" mass="46164" checksum="B2AA3CCED04CE600" modified="2014-04-16" version="1">MTTKQVTCRYFLHGVCREGTRCMFSHDLTTSKPSTICKFYQKGQCAYGDRCRYDHIKTAAGRGAGAPGDLTEKSCSGLAAVAGAIPPAGSGRRGKRSLVLRDRALCSTSEVRMPQHRSDASDSSKFDCWGHSDEAVNPKPHSYLEAIQSGLESSASGSSVTDSQQLCPYAAAGQCHYGNSCTYLHGDVCDICGLQVLHPMDSEQRKAHEKLCMMAFEVDMERAFAAQFSQDKVCSICMEVVYEKAVPSEKRFGILSSCNHTYCLSCIRQWRCAKQFENKIIKSCPECRVVSEFVIPSSYWVEDQDEKNRLIEEFKAGVSKKPCKYFEQGRGTCPFGGKCLYMHAYPDGTRAEPDKPRKQLSSEGNVRFLNAVRLWDFIEEREQRGARQPDDEAVAELGELFLQLSGADGEGGAPASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBF6</accession>
    <name>W5NBF6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Intersex-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 29</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 29 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBF6"/>
    <dbReference type="FunCoup" id="W5NBF6">
        <property type="interactions" value="1216"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017965"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017997.1">
        <property type="protein sequence ID" value="ENSLOCP00000017965.1"/>
        <property type="gene ID" value="ENSLOCG00000014598.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRNJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164885"/>
    <dbReference type="HOGENOM" id="CLU_101133_2_0_1"/>
    <dbReference type="InParanoid" id="W5NBF6"/>
    <dbReference type="OMA" id="NHYLPGP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014598">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042462">
        <property type="term" value="P:eye photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021018">
        <property type="entry name" value="Mediator_Med29_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28314">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28314:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11568">
        <property type="entry name" value="Med29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="171" mass="18711" checksum="4CCB38FB2CFB88D2" modified="2014-04-16" version="1">NMASQQQQPQMSQAGGPQVQSGLQAPTQDFDPVHRFKMLIPQLKESLQNVMKIASLNLGHNTAIDNGVKSSDASVQRFDKSLEEFYALCDQLELCLRLAYECLSQSIDSAKHSPNLVPTATKPDTVQTESLSYSQYLSMIKSQISCAKDIHNALLECSKKIAGKGPSQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBP4</accession>
    <name>W5NBP4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/nucleoside cotransporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBP4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018053"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018085.1">
        <property type="protein sequence ID" value="ENSLOCP00000018053.1"/>
        <property type="gene ID" value="ENSLOCG00000014663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697608"/>
    <dbReference type="KEGG" id="loc:102697608"/>
    <dbReference type="CTD" id="9154"/>
    <dbReference type="eggNOG" id="KOG3747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016025"/>
    <dbReference type="HOGENOM" id="CLU_016813_3_0_1"/>
    <dbReference type="InParanoid" id="W5NBP4"/>
    <dbReference type="OMA" id="IVWHTVI"/>
    <dbReference type="OrthoDB" id="6075923at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014663">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005415">
        <property type="term" value="F:nucleoside:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901642">
        <property type="term" value="P:nucleoside transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018270">
        <property type="entry name" value="C_nuclsd_transpt_met_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00804">
        <property type="entry name" value="nupC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="SODIUM/NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590:SF22">
        <property type="entry name" value="SODIUM_NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="590"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter N-terminal" evidence="9">
        <location>
            <begin position="204"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="11">
        <location>
            <begin position="285"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter C-terminal" evidence="10">
        <location>
            <begin position="388"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="683" mass="74713" checksum="4E6EF522CEDB8EC8" modified="2014-04-16" version="1">MDLKSLTSLDNSDGQGKDNLGFDLNEEGQSCRVKTYPTQTSTSFSSGNDTDHAVTELQGEGQTNGINNDPHDSSTAHRYWSKISQPFSKAKRYCKSRGRLIKNIILAVLAAGYLAYFIAACVLNFHRAIALVVLTGLVVFFSLYDLVKKKWGKQICKCFKPVGQCFRNNLRWMRWVFIVIVLGLLVAWLVVDTSKRPEQLISFAGVCMFILLLYIFSKHPKKVSWRALFWGLGMEFALGLFIIRTNPGFVAFDWLGKQVQIFLNYTTAGSGFVFGDALINQAFAFQALPIVVFFSCVMSVLYYLGLMQWLIVKIAWIMQKTMGTSATESLSVAGNIFVGQTEAPLLIRPYLPEMTKSEIHAVMTGGFATIAGSVLGAYISFGIDASALIAASVMAAPCALALAKLSYPETEASKFKSEKEIKIDVGDEKNILEAASSGASASVSLVANIAANLIAFLALLEFMNAALRWLGGMVNYPKLTFQLICSYIFMPVAFMMGIPYEDSFIAAELIGTKLFLNEFVAYEKLSVLKKNRLNDLDKCINGELQCISVRAETICTYALCGFANFSSLGIMIGGLSSISPTRKSDIAEGVLRALFTGTCVSLVNACIAGVLFVPPSDCIRVFQNSTFSSTTPDLDSCCLDLFQSTSWNNGTAGFDEAWNGVQNAAQYLLNCCNLYNSTACTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEJ6</accession>
    <name>W5NEJ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LHFPL tetraspan subfamily member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029184">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEJ6"/>
    <dbReference type="FunCoup" id="W5NEJ6">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019055"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019087.1">
        <property type="protein sequence ID" value="ENSLOCP00000019055.1"/>
        <property type="gene ID" value="ENSLOCG00000015483.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Linkage group LG8"/>
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    </dbReference>
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        <property type="entry name" value="LHFPL"/>
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        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
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            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="234" mass="25476" checksum="4A374147DDC0093C" modified="2014-04-16" version="1">MVPGTPAAMLPAQEAAKIYRTNYVRNSRAIGVLWAIFTILFAIVNVVCFIQPYWIGDGVETPQAGYFGLFHYCIGNGMSRELTCQGSFTEFSSIPSGAFKAASFFIGLSMVLVITCIVCFALFFFCSTATVYKICGWMQLASATCLVLGCLIFPDGWDSDEVKRMCGEQTDKYTLGACSVRWAYILAIMGILDALILSFLAFVLGNRQDGLMAVELLADSKGNSLLMPVSSVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF05</accession>
    <name>W5NF05_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Carboxypeptidase A1</fullName>
            <ecNumber evidence="15">3.4.17.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.</text>
            <dbReference type="EC" id="3.4.17.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.1" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01016138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF05"/>
    <dbReference type="FunCoup" id="W5NF05">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019214"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019246.1">
        <property type="protein sequence ID" value="ENSLOCP00000019214.1"/>
        <property type="gene ID" value="ENSLOCG00000015607.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158082"/>
    <dbReference type="HOGENOM" id="CLU_019326_0_0_1"/>
    <dbReference type="InParanoid" id="W5NF05"/>
    <dbReference type="OMA" id="LGYTIMI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015607">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03870">
        <property type="entry name" value="M14_CPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000132">
        <property type="entry name" value="Carboxypeptidase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000001">
        <property type="entry name" value="Carboxypeptidase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034248">
        <property type="entry name" value="CPA_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF94">
        <property type="entry name" value="CARBOXYPEPTIDASE A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867525" description="Carboxypeptidase A1" evidence="18">
        <location>
            <begin position="17"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="19">
        <location>
            <begin position="124"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="17">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="422" mass="47940" checksum="DE46304BBB3D7FE4" modified="2014-04-16" version="1">MKGLLLLAALFVAVFSQKLFVGDQVLRVTAKDEDQITLLQELSDLEDLQLEFWKEPRHPDLPIDVHVPFASLQAVKVFLESNSISYTVMVKDLQQALLDEEQHQMKRFRSMPQDHNTDSFVYSTYHTLDEIYGWMDTLVSEHPNLVSKIQIGQSFEGRPLYVLQFSTGGADRPSIWIDTGIHSREWVTQASGVWFAKKIATDYGRDASLTSILNKMDIFLEIVTNPDGYYFTHTSNRMWRKTRSTNPGSSCRGVDPNRNWDAGFGGAGASSNPCSETYRGPYAHSESEVKSIVDFVQSHGNFKAFVSIHSYSQMLLYPYGYTRTPARDQEELHNVATKAVTALSSLYQTNYRYGSIITTIYQASGGTIDWTYNQGIKYSFTFELRDSGRYGFLLPANQIIPTAQETWLALMAIMEHARDNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF12</accession>
    <name>W5NF12_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myelin and lymphocyte protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MAL</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF12"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019253.1">
        <property type="protein sequence ID" value="ENSLOCP00000019221.1"/>
        <property type="gene ID" value="ENSLOCG00000015614.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154987"/>
    <dbReference type="HOGENOM" id="CLU_112950_0_0_1"/>
    <dbReference type="InParanoid" id="W5NF12"/>
    <dbReference type="OMA" id="YATHIVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015614">
        <property type="expression patterns" value="Expressed in embryo and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013295">
        <property type="entry name" value="MAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF12">
        <property type="entry name" value="MYELIN AND LYMPHOCYTE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01884">
        <property type="entry name" value="MALPROTEIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="18"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="154" mass="17058" checksum="175C1E8AA9ECC8A3" modified="2014-04-16" version="1">TAGDMATPSSSLPSGCGVFTTVPDVFFLPEFVFGGLVWILVASTKVYLENPQGWVMFVSVFCFVVTTLWFFLFISGVNQRSTVWSTLDVAYHALAAFFYLSASVALANVTIRLQNDSLNFKSYQEDIAAVVMSYIATLFYVLHAVFSAIRWKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFQ5</accession>
    <name>W5NFQ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 26 member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFQ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019464"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019496.1">
        <property type="protein sequence ID" value="ENSLOCP00000019464.1"/>
        <property type="gene ID" value="ENSLOCG00000015808.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286920"/>
    <dbReference type="HOGENOM" id="CLU_003182_9_4_1"/>
    <dbReference type="InParanoid" id="W5NFQ5"/>
    <dbReference type="OMA" id="IVCMAVK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015808">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140900">
        <property type="term" value="F:chloride:bicarbonate antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019531">
        <property type="term" value="F:oxalate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015116">
        <property type="term" value="F:sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902358">
        <property type="term" value="P:sulfate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="527"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="771" mass="85037" checksum="3C942E0233A8A932" modified="2014-04-16" version="1">KEIMDRSPEHNYFVVRPIYSKPTFEEDNETKARVPKTFRDKLQKSCTCSSKRALRVVKSMFPILDWLPNYRVKEWLPSDIISGVSTGLVCTLQGLAFALLVAVPPRYGLYAAFFPILPYFILGTSRHISVGPFPVTSLMVGSVVLTMTPDSNFIKTINGTSGNGTAIIDTNARDAQRVLVAITMTYLIGFFQLGMGVLQIGFIVRYLSDPLVGGFTTAAAFQVFVSQVKIILAVPTGNYNGVLSVIYPIIDIFKNIRQTNIADLVAGLLTILIVMVVKEINTKYQHKIPVPIPIEVIVTIIATGISYGVDLKSKYNANIVQNIPRGFLPPQNPDVTLFSSIVSSSFSTAVVAYAVAVSVGKVYATKHDYAIDGNQELLTFGICNIFTGAFSGFIATTALSRTAVQESTGGKTQIAGIISAALVLVVIFALGSLLEPLQKSVLAAIIIANLKGMFMQVLDVPRLWRQNKIDSIIWVFTCIGCIILGLDLGLLAGLVFELVTVVYRTQFPTCSALGNFPGTDIYKNIKDYKHVAEIPGIKIFKCNSPIFFANIDYFKDRLKAVVGVDAARVLKKRTKALRMIQNLIKKGRLRATKKGVVSTMGIDNEGFEMEKELEEMEESSIPTTEVEIRVDWDSDLPVKVAVPKVSFHSLILDFSAASFLDVVSVKSLKLVIKEFQKIGVNVYIAGYDDEIAQKMEFCGFFDDAVKKDIMFISVHDAVLFIKIQTINGAIQDPIFEKISLMQDSKEPLTFTDDETEETYDNQRRAIHGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGK5</accession>
    <name>W5NGK5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01021026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGK5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019797.1">
        <property type="protein sequence ID" value="ENSLOCP00000019764.1"/>
        <property type="gene ID" value="ENSLOCG00000016045.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183090"/>
    <dbReference type="HOGENOM" id="CLU_008917_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGK5"/>
    <dbReference type="OMA" id="WWVEVRM"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016045">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="495" mass="56761" checksum="F913F8527ED8C80E" modified="2014-04-16" version="1">MAEKRNSKDYILLFLIIFAIFVHLLICPFTKVEESFNLQAMHDILYHRLNLEKYDHHEFPGVVPRTFLGPLFISALSSPLVYLLYILDAPKFYSQVIVRGCLGLCVTFALWKLQREVRKQFGSTAAALFCFMCASQFHLAFYSSRTLPNTFALPIVLLAFTFWLQQNLSIFIWLSAFSIIVFRSELCIFLGLMLLMSLMSRRLSILQLLYMAVPAGLFCLALTIGIDSVFWKQLLWPEGQVLWYNTVLNKSSNWGTSPFLWYFYSALPRALGSTILFVPLGVLDRRTRMFLLPAVGFVFLYSFLPHKELRFIIYTFPIFNIIAARGCSYVLNNYHKSWIYKVGSLLVIGHLAANVVYTGMSLYISHFNYPGGKAMEQLHLLIPPSADVSVHIDVASAQTGVSRFLELHDNWKYDKREDIIPGDSEMEEFSHILMEADPTKLIPLKETHKIIAFIPCFKELVLNVFEVPPIHIKLENKLVLLEKISHTTPVTLGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGW9</accession>
    <name>W5NGW9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Interleukin 17 receptor A</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGW9"/>
    <dbReference type="FunCoup" id="W5NGW9">
        <property type="interactions" value="1227"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019878"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019911.1">
        <property type="protein sequence ID" value="ENSLOCP00000019878.1"/>
        <property type="gene ID" value="ENSLOCG00000016133.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV5J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159018"/>
    <dbReference type="HOGENOM" id="CLU_018087_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGW9"/>
    <dbReference type="OMA" id="WRAMCGD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016133">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11530:FF:000002">
        <property type="entry name" value="Interleukin 17 receptor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2160:FF:000002">
        <property type="entry name" value="Interleukin 17 receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2160">
        <property type="entry name" value="Interleukin-17 receptor A/B, fibronectin-III-like domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2150">
        <property type="entry name" value="Interleukin-17 receptor A/B, fibronectin-III-like domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032356">
        <property type="entry name" value="IL17R_A/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038683">
        <property type="entry name" value="IL17RA/B_FnIII-like_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043046">
        <property type="entry name" value="IL17RA/B_FnIII-like_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF13">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16556">
        <property type="entry name" value="IL17R_fnIII_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16578">
        <property type="entry name" value="IL17R_fnIII_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867734" evidence="10">
        <location>
            <begin position="21"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="11">
        <location>
            <begin position="346"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="642" mass="72994" checksum="EB860C75ADEADED3" modified="2014-04-16" version="1">MDLLLCRLGWISFWITLTASVRILEIPPINCTQNGLKCTAVKSNCLHSSWLQFMDCTPTNPLWKSEPEVGIRMDENGDEVPVMVINWRAQIDGSIRCLNGTQVHVLKLSNNDHFCVQFIFQNELKNMLNPDGNAWSFSLDRIVAEPGHTYMISVSNLPKPNIGADYYHIAKNLSVPECSDHRIRRTKLCQQKGSLWESNINLVVSGEDATIHFNTGNYSEKYVVFFKCSENDKDHKSVMKENQTSRSVTFSLSKWSPTCCNFNVEVQPFFVGCENDCIRHRKSFSFCQGTDDPNVQPNKFPYWVIPALGGVLALVVACFFFLVCRKRRKGVEPLIHNVNLDAPRRPRKVLILYSLDHPLYKEIVLKLYAFLKAECGIEVILDLLDTTWLGVVGRMQWLDWQKQQIEKSSDKILILCSRGIRAKWEAMCGNGKVLLKEDVRSSMGDMLTPALSLIIPDFLGSASFGKYMVAYFEDVSSEQDIPGPFRITVKYKLMKHFEEIYFRIVDKEKHEPGRINRVEGISQDEYCKRPCGKALQDAIDAFQAYQVENPDWFEQECVDSEEEVAESYPVDTAANSVLCCVPQLKEGLPILINEVGILRDTEELYAVTPLVQDEGESLLSCVVLPGVHPDNYQVLTSQPVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI51</accession>
    <name>W5NI51_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (32-2'-O)-methyltransferase regulator THADA</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020310"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020344.1">
        <property type="protein sequence ID" value="ENSLOCP00000020310.1"/>
        <property type="gene ID" value="ENSLOCG00000016430.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015500"/>
    <dbReference type="HOGENOM" id="CLU_002048_0_0_1"/>
    <dbReference type="InParanoid" id="W5NI51"/>
    <dbReference type="OMA" id="CTSIPEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016430">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF7">
        <property type="entry name" value="THYROID ADENOMA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="7">
        <location>
            <begin position="530"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="6">
        <location>
            <begin position="954"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="8">
        <location>
            <begin position="1255"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1008"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1008"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1928" mass="211478" checksum="B170833E64E96623" modified="2014-04-16" version="1">KMVLKKKEVKVEALILDSGQLENLGALKGDPLAEQIAEFLQECAKLTDGVQQIQLIKKVGSLLEQLSEDEAESPLMNACLETLACVFFSLHSRNPLKRALASALSTVPGCLQNQAVRCLSQRLSQSLTTCSSTHYAHLTDTITACLDGFHLATGESCIHSLLTEVLQFLQKALSEYQQQNSRLVGSPVAQAQLMQACLATVKASMLVVQRSQKQITAVLQAAPDSPLMDTLCDLLRCYTSILTEGDFVQTVQSTAGMAVVLLTRSMTGPSDCVASVVASLLLGSEERVGSAPQWLLESCGELYHCVRPTSVTLFLCHGALAMLSWRGKAARSGEERLLLLIPKALLSLDSSVRESSTAISVARVLTLWSCAALEVLQGEHCSASLRHSLTGGTELVGRLLEHIYAHWEHPLDGVRHQTKALFKNVLKLHQIATRIPDPLADPFITSLTHSLLGLEWHSKGKYGSLSCLVDYLGAESLLALDPQLPAQLLSLMGDQSLAPYASSLLETLFVNHKAQLASCHERGDSWMEMWHQTWVTPLLAALCEGQLDQTTYILDYCLPKILRCSPGSVEHMVRALQSSQHSPAGNNTDCRGALGALVTCLRAARAQGVVSSSQEGLWGGLVPLKLLSQALVHKHDQVRLDALGLICESHRSTENLSPEEMDLIRFFLPSSLNSQSPGVRQQTLSLLKKLLCRMRDSAQLLQRRLEQDRPEEQGQEHGNRTTLQTYEEFLHWLCEALFQALFPGASFPTCFTALHLLGLIADLFSFSTADSGPCLFSLGQMVTPSQAQAVLYCLASSFLEVKQLAFQLLRRLPPPALGLQEPGRLSSLLRVALDLSTSTKPFDSVTASHLLNLLVYEEGLVEALSHRAQEQCISFQPPTPGESPASKAFKLERNTLAVVLFLLACLKAEVAQAETSLLQAAASYPLYGRAHCITVALQQLDSQSLLLVAEWRPVVAELIEMSYRMSDVVSPVVQSSSPEGLIPMDTDSESSACLQQILQEIQPRDTNDFFTSPRQLGNSEPEQGGGSLEQAHAAGATGGGEEQYRVTAQMVLVCCWRTMKEVSMLLGMLCQNLPLQTEAPPREGLITEQQVEGVGRYFRQQLLQSRHRGAFELAYVGFVKLTDMLARSPGRALQQLPGRWLSEVLEEVRSSGPTSKLCATRRSAGIPFYIQALLSSEPKSSSCSLLRMTMKELTALALSMQDNPTDGSSVPQVHSLNILRALFRDTRLSENIVPYVAEGVQAAILGFTSPVWAVRNSSTLLFSTLITRIFGVKKGKDENSRKNRMTGREFFTRFPALYPFLLRQLEAVASTVDSDSGVLKLHPNLFLLLLLLGKLYPSPMDGFSSPLGLAPFVPFIARCGRSPVYRSREMAARALVPFVLVTQVPSMIQTLLQDLPECPRSATRQNHIHGTLLQVLHLLRSYQEDRHRPHSLRLPQTHDIIAAVRARLWLARRQNPCVVTRAAFLDVLACLCGPEWGPVDDEELTGLREATLVIVSDSELFDPDSFSTAVAGGTQYLLSLARIAVCASLENPVLWGAEFQGPQLLAQLLQCQVYEVRLLALEAILQQLERGPSDRHPAPCPLSTVTTILTNLAMQETHPQCMGKVLRALSLRSVASILPWQKGSEMLGEEELLHWALTVCQESTHRVENHCAALTLVSNFVVHFVEKSSQGLSKSMQLEMRTWVALVSQCCGDERPGEVKLTAAEVLVNATPCLLASAVLPLGLSDTVCLWKSLFTLLQDEDQDVRDRAADFTGNVPPQLLTDQHTGFAPSALCPALALDLGLGLLCQLFQMWKEAPAGVLILAEWLLGEGDPEREAEDGRVLDEDEFLFEKGEGNLWAERLLWVRLLDKHLSGLVSVPGTLSGIQEELVQLFGLARNLAQTSDCLLQALPPEPQFSSSADFTRLTIQRERTARALETLKATRPATLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI83</accession>
    <name>W5NI83_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tribbles homolog 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626004.1">
        <property type="nucleotide sequence ID" value="XM_006625941.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI83"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020342"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020376.1">
        <property type="protein sequence ID" value="ENSLOCP00000020342.1"/>
        <property type="gene ID" value="ENSLOCG00000016468.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684186"/>
    <dbReference type="KEGG" id="loc:102684186"/>
    <dbReference type="CTD" id="28951"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182986"/>
    <dbReference type="HOGENOM" id="CLU_000288_13_1_1"/>
    <dbReference type="InParanoid" id="W5NI83"/>
    <dbReference type="OMA" id="CFCLPPH"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016468">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000153">
        <property type="entry name" value="Tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000253">
        <property type="entry name" value="tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF15">
        <property type="entry name" value="TRIBBLES HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="61"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="345" mass="39096" checksum="BF1711680BC1C194" modified="2014-04-16" version="1">MNIQRSSPINITRYGRSRHKNHDFEDLSCLRTTESIQSFSPNLGSPSPPETPNSSHCISCIGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFDIARYQESLAAYFCLSGHENINQIVEILLGETRAYVFFEKSHGDMHSFVRTCKKLREDEAARLFHQIASAVAHCHDNGLVLRDLKLRKFVFKNEDRSLVKLESLEDAYILEGEDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTILVGRYPFHDVEPSSLFSKIRRGQFSIPETLTPKAKCLIRSILRREPTERLTSREILDHPWFSSDIHASNSGYGANEKEASDQLVPDVNMEEELDKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIN4</accession>
    <name>W5NIN4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sine oculis binding protein homolog (Drosophila) a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Implicated in development of the cochlea.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SOBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIN4"/>
    <dbReference type="FunCoup" id="W5NIN4">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020493"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020528.1">
        <property type="protein sequence ID" value="ENSLOCP00000020493.1"/>
        <property type="gene ID" value="ENSLOCG00000016593.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QZ8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154164"/>
    <dbReference type="HOGENOM" id="CLU_012732_0_0_1"/>
    <dbReference type="InParanoid" id="W5NIN4"/>
    <dbReference type="OMA" id="MAPCVIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016593">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026092">
        <property type="entry name" value="RAI2/SOBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23186">
        <property type="entry name" value="RETINOIC ACID-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23186:SF2">
        <property type="entry name" value="SINE OCULIS-BINDING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15279">
        <property type="entry name" value="SOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="587"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="719"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="754"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="899" mass="97322" checksum="E137EF65D4AE51DB" modified="2014-04-16" version="1">MAEMEKEGRPPENKRSRKPAHPVKREINEEMKSFAENTMNELLGWYGYDKVELKDGEDIEIKNYPDGESRQHISVLKENSLPKSKALDDSINSPTNANSTNSGPTPGNRMAESSSSSSASSSKDHGNVPIIVPLIPPPFIKPPAEEDVSNVQIMCAWCQKVGIKRYSLSMGSELKSFCSEKCFAACRRAYFKRNKARDEDGHGDKFPQQHFSKETPRLVFKINNDVLQVQLDRYDHIIRQLRGSDKTRPSKDISVELIEEVCDWCKHIRHTKEYLDFGAGERRLQFCSAKCLNQYKMDIFYKETQANLPAGLCNPGHPPMESKPESSNLQLLTPDSWNTPLGDLRRKAPSPGTSMAGHNQAPGPSGSASASPSEATAICSSAKIPTPGPKPHESPTLPPPVPIPAPPMGVPSGSPPMVMTHRGPIPLPFFMDHPMLPQIRSPFLRAPHTPSPNSPLPNPIIPGIGPPPPPRNLGPTSSPMHRPMLSPHIHPPTTPTMPGNPPGLMPPHPGAPMPGLPFPPVNMMPNGHMPMPQMMNFGVPSLAPLVPPPTLLVPYPVIVPLPVPIPIPVPIPFNSKASAGLRNNSENIIPSTSSEAGFSGHLLNPSPPEQGGRTEVVDLTVKAESAVKSRLSLTNLPALPQDGVIDLTVGRRSRLQHVIQRAVPSTQVKVEHEPPHSVLNLTFLSEDKRNCSGLDDESRDSCSPVDPLALPCSDPAHCGAPPAVPPPPPQQQHTLMSSSSGPEPSPAAVCNVIVNGTKTPDSTSKSASSEQRPEPRKTAQQLPLEEPAVSELESVKENNCTGAGGFQMELDTGKKVSSEEPLGSGDKQDPNLNNPADEDHAYALRMLPKAGCVIQPVPKPTEKTAITPCIISAPLLSTGPEDIEPPLKRRCLRIRNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIX0</accession>
    <name>W5NIX0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-39kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase 39 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction. Interacts with BLOC1S1. Interacts with SLC2A4. Interacts with CLOCK. Interacts with RAB5IF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the complex I NDUFA9 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633741.1">
        <property type="nucleotide sequence ID" value="XM_006633678.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIX0"/>
    <dbReference type="FunCoup" id="W5NIX0">
        <property type="interactions" value="1910"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020579"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020614.1">
        <property type="protein sequence ID" value="ENSLOCP00000020579.1"/>
        <property type="gene ID" value="ENSLOCG00000016659.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695927"/>
    <dbReference type="KEGG" id="loc:102695927"/>
    <dbReference type="CTD" id="541331"/>
    <dbReference type="eggNOG" id="KOG2865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006865"/>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="InParanoid" id="W5NIX0"/>
    <dbReference type="OMA" id="PEDQFTN"/>
    <dbReference type="OrthoDB" id="275457at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016659">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000246">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="16">
        <location>
            <begin position="59"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="42909" checksum="93AB7D9EB352787D" modified="2014-04-16" version="1">MAAVALRCRPAGVLSRFSCAGSPGVISAVSIVVQQRKIHHAVIPRGKGGRSSFSGIAATVFGATGFLGRYVVNRLGRVGSQIVIPHRCDQYDIMYLRPMGDLGQIIFLEWDARDKESIRRALAHSNVVINLVGREWETRHFSFEDVHVNIPKQIAKAAREAGIEKFIHMSHLNADIRSPSRYLRTKAVGEAVVREEFPDAIIMKPSELFGREDRFFNHFANMRWFGKAIPLIARGKKTVKQPVYVVDVAKAIVGAIKDPDSNGKTYALVGPNRYLLHDLVEYVYAVAHRPFICYPMPRPLYHLVAGFFELSPFEPWTTRDKADRFHTTDMKFPALPGLQDLGIVPTPVEQKAIEVLRRHRRYRWLEAELGETKPAKTVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJL7</accession>
    <name>W5NJL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myomesin 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NJL7">
        <property type="interactions" value="916"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020826"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020862.1">
        <property type="protein sequence ID" value="ENSLOCP00000020826.1"/>
        <property type="gene ID" value="ENSLOCG00000016832.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS6D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157057"/>
    <dbReference type="InParanoid" id="W5NJL7"/>
    <dbReference type="OMA" id="ASFRFVI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016832">
        <property type="expression patterns" value="Expressed in muscle tissue and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000029">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000197">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000222">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000233">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002172">
        <property type="entry name" value="Myomesin 1a (skelemin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000670">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF22">
        <property type="entry name" value="MYOMESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="144"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="259"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="375"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="503"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="604"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="703"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="805"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1120"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1348"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1441"/>
            <end position="1465"/>
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        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
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            <begin position="1443"/>
            <end position="1461"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
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    <sequence length="1483" mass="167613" checksum="FA8E96591E8639E4" modified="2014-04-16" version="1">MATKVAPFYQRKHKHFDQNYRSTQTRYVVQEYAASSRSRTSAVKSSSKSRLSAQSKRFHAAEEEQQQEVTYTVPPFRTRSAEEQEEYQRRVVHFGNDLAVLEGEVHRARQATREQVDKLAVQRMVEERMAAERYIHEQSMVRGPDFLVRLRSHTVWEMSPVRLFCTVEGYPSPVVKWFKDDVILDPISMMGKYRIDSKYGVHSLEIHRCDIHDTAQYTAVATNAHGQATSTAAVIVKRYRGEEEPYHSVLLPLQIPMMPEIEYTKIDISFVEKFDVTFGTEGETISLVCKMVIAPDLANLQPEAQWYRDDVRIKESKWAQMKCGGGSARLTLTHLNKDDEGLYTLRMVTRGGTSEHRAYVFVSDAPATVAGAPGAPMDITIHDANRDYVIVSWKPPNITSESPVIGYFVDRCEVGTNNWVQCNDSPIKICRYPVHGLFEGHSYIFRVRAVNKTGVSRPSRVSDAIAALDPADLERLHVIHLERGKQIVIHQDDLEGDVKIPGVPTNVHAAETNRTYVVLSWEPPVPRGREALMYYIEKCMVDGGNWQRVNTQIPVRSPRYAVFDLAEGKRYLFRVLSANKHGTSEPSEPTPPIQTEELHSVPSAPGRILATRNTKTSVVVQWDPPKQAAGLIGYYIDSSLLGSKQWAPCNHKPYKHTRFVVHGLTTGQSYIFRVKAVNELGFSEDSQESSAISVQAALTSPSPPYGITLLYCDGHSMTLGWKHPKYSGGAKVTDYYIDQREVSHLHWRETHVAPIKERIYKVENLQESKIYEFKIQAANLAGVGLSSDTSDPFNCEAWTMPEPGPSYDLSFCEIRNTSLVILWKAPLYAGKSPVTGYYVEMSTVGSEVWTTVNEKAISHCYLKVTNLEEASSYMFRVRAVNAAGKGKPSDVSEPVFVQARPDTKEVSSGVDEEGNIYLKFECNEMTDISQFIWSKSYEEIVDFSRVSIETEGTFSKIVFKNPDKEDLGTFSVAVTDTEGVSASYVLEEKELEKLMALSHEIRNPLIPLKKELSYEIFERGQVRFWLQAEKLSSAVNYTFIVNDREVTSSESHKIHCDISTGIIEMVMDKFTSQSEGTYTVQIQDGKAKNQSALVLIGDVFKAALEEAEFQRREYLRKQGPHFLEYIHWHVEEDCTVMLVCKVANVKKESVFQWFKDDEKVILDEPVDVMSGICKLPIPQFSKKDQGEYRATLKDDRGQDVSTLDVSGKVFEDIINGISKIAGASASELKIQCTPEGIRLQCFMKYYTEEMRTVWYHKDSKISSSEKMRIGGTSEMVWMQICEPTEREKGKYTIEILDAKQSYTRTIDLSGQAYTDAYAEFQRLKQVAFAEKNRGKVVGGLPDVVTIMEKKTLSLTCTVCGDPSPEVVWYKNDKEVELDDHYVVTLEQGKYASLTIKGVTTEDSGRFSMNVRNKYGGETVDITTCCKGSWLGRYAGSTVRKRLEKGDRETRQDPDDMTDGGRGDSVPVMGGVAAVFRRWGAWLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJU0</accession>
    <name>W5NJU0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sentrin-specific protease 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">SUMO-1-specific protease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sentrin/SUMO-specific protease SENP6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Also desumoylates RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with RXRA. Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation. Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015210480.1">
        <property type="nucleotide sequence ID" value="XM_015354994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJU0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020899"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020935.1">
        <property type="protein sequence ID" value="ENSLOCP00000020899.1"/>
        <property type="gene ID" value="ENSLOCG00000016904.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686455"/>
    <dbReference type="CTD" id="503939"/>
    <dbReference type="eggNOG" id="KOG0779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155724"/>
    <dbReference type="InParanoid" id="W5NJU0"/>
    <dbReference type="OMA" id="HYHESAA"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016904">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090234">
        <property type="term" value="P:regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090169">
        <property type="term" value="P:regulation of spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="sentrin-specific protease 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000010">
        <property type="entry name" value="sentrin-specific protease 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="18">
        <location>
            <begin position="672"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="324"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="811"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="847"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="927"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1079"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="847"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="871"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="883"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="951"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1108" mass="123858" checksum="3D667C172CD14480" modified="2014-04-16" version="1">MAHKRSLFIEALDRSEAKKDGGFKQTWSFSVADGSDEERQNDSDSVVSMDEPDGMNSPEGRKPTPLKHFCTGEPLKTYERRPNNHLRSFKGNAFGLNMLGAGKKLGPSAPRSVFLHGRYFQHANAPAPVVKTAAQSLDLKERKEYPPQIQRLELDSIILTCPETTEANSMVHEGEIKRQTQQKRRASSEDLISFVSSTKNVETLDPHEYLTVCGKCGKPSEDHVKCENCGNILAQDSAGSLKTSTNLAGTASPLSRPSIRRHSSGPNHLLLSKNAFSSSLTIKASQVDVGSSPPVRVTRSNLLISNGRAPLVTGTSPLVAKISKGKRQMETKQHELNDPIVLSSDDEEEEADNASTGSVNRMDSISPRPADSAHSSPAPSGGKVEAAVKDNTEQPDWLTDDYFTDVDARITLPRKARMKDQFGNVVSDKSITKRRKIGQSNTSTNSTKQPPMLESIILDCRSIRIGTLRKAVVKPVIFTVDYIQIETEASTVDKMQTITLKTSEISCCEWCNVRKLPVIFFQTTQSECFRLQDLLQMSKENGGVWYDCKGPNLDEKYIVLIFENGLSLREQAILEEILQEIGKANNIEDFLIKIPFDEANIRLVRFTKSCPDSASKEKGSESSPRVPVSTRLHASAQRYFDDDEEMAELHPTFTGPIEKLIVYPPAPAKGGISVTNEDLHCLNEGEFLNDVIIDFYLKYLVLEKLKKADAQRSHVFSSFFYKRLNQRERRSVVDPTNLPIQKRRHNRVKTWTRHVDLFQKDFIFVPINESAHWFLAVICFPGLDGPQFEPNPLYRTSESILVNSSTAEESLSSAAEEVENSTERFPSSPAFQASKCPVTKALIVSTTEESSESDCNTDQVKGVAVPSNSVKDNERANTHEFRNTQSSTTTGVNGFRADKQKAPQQCIGGLQRIRLCYGNSKNRDDDTFTFSDDQDSSLDESSEDGTLADDTLTSSESGQWNQKPTICKQPCILIMDSLRGPTRSTVVKTLREYLEVEWEVRKGNKRSFGKDVMKGSSPRVPQQDNFSDCGVYILQYVESFFENPLPSFHLPMHLTDWFPQQRMKTKREEIKELILKIQSQQQADKKGHQDTEAEIPGRSVLQNPISDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKM0</accession>
    <name>W5NKM0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01037825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKM0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021179"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021215.1">
        <property type="protein sequence ID" value="ENSLOCP00000021179.1"/>
        <property type="gene ID" value="ENSLOCG00000017137.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286899"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_2_1"/>
    <dbReference type="InParanoid" id="W5NKM0"/>
    <dbReference type="OMA" id="EHEENIH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017137">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="163"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="513"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="720" mass="80393" checksum="BF6FD217B4F8ED5A" modified="2014-04-16" version="1">RLLILLFCCEKNKMAEANVSVAQDQFSCSVCLETLKDPVSLPCGHSYCMGCIKSHWDQDDRTGVYSCPQCRQTFSPRPVLGRNTLLAEVVEKLRKRGSSTPLPPPAHSPAGPGDVLCDFCSRRKMRAVKSCLVCLASYCQTHLQPHYEAPAFKKHRLTSVSRHLQEICPDHQKLREVYCRTDQTCVCLLCSDKDHRSHDTVSTETERAEKQKQLGATQRQTQQRLEEREKELQELRQAVESLRSSGRRAVEDSERVFNELVRSVLRLGSQVKEQIEAGEREAVGQTERHIEELEQEIAELRRRDAELSQLSHTEDHIHFLQTFQALSSLWPGVSRKAPVSAESTFGAVRRLVSELKELTEDLCRQILVRIADAGGRRASPLAAAAGCQPGASGALLSYPGLQEHRLYKTDPHPPFLTAQLWAQQCHTPPPGGSISLCVPSPPSNEIPFLSLSLPVKLRSPSRSLQRPRGESSTVRDAQHTKSTVRDAQHAESTLTAPLETHSTPRAPLETHSTGVHTAGCPCMREDTAPLSCQLTLDPNTAHRHLCLSEGNRRVTWSREPQQYPDHPERFDHWQQVLCREGLSGTRCYWEVEWSGGCVAIGVMYRGISRKGRNVPSHLGHNDQSWSLCCSGSSCSFWHNKEKTAVAAPPSSRIGVCVDHRAGTLSFYSVSTALSFYQRLWSLYSAPETMSLLHRVQTTFSEPLYAGFMVGSDCSLSLPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL40</accession>
    <name>W5NL40_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Triadin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025913">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL40"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021349"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021386.1">
        <property type="protein sequence ID" value="ENSLOCP00000021349.1"/>
        <property type="gene ID" value="ENSLOCG00000017261.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_603495_0_0_1"/>
    <dbReference type="InParanoid" id="W5NL40"/>
    <dbReference type="OMA" id="TRTHESS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017261">
        <property type="expression patterns" value="Expressed in muscle tissue and 4 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867779" description="Triadin" evidence="2">
        <location>
            <begin position="17"/>
            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
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        <location>
            <begin position="156"/>
            <end position="179"/>
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    </feature>
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        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="229"/>
            <end position="310"/>
        </location>
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        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="382"/>
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        <location>
            <begin position="393"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="51293" checksum="6A7FED89F7A7A15E" modified="2014-04-16" version="1">VSLISVFLFFPLPAHTVIKKVKDAKESKARDEKKAPPKEVRRPKIEKKEERVKREQKPEKKVEKAKKEVKAVEKAKSKAKPKEEKPVKKEPKEKRPVAKSKEEKKDEPKKKPSREEKKTAEATKTSTKHLRKAEPKVEESAPPKETARRKQPPIKQSERKVEKKPEAKPKAAQHTEETPPKIVTGSHQSKLEKVEEKDAKPEKVVKREEKLSTIKVSKVKKEQTKRKKTEISKEKARPPPLKHEPVVAKEKARTLTPKKGTEVAKEKPRPSPPKKESEVAKEKAHLKKVSEVKKEDKLSKGIDQESEKQKTKPASSAEKKESEAVKVEKPAKQESKAAKVEKPAQQVKKAKGKVTKEKAKEIVHTAEAKLTKSLHPPKEHPPVKAPSTQREAINQEKETPSAKSEHLVEAPETGKHTEVDKKPAKEEKVKEKDLKTSKPTTSTFAEKVVNQSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL94</accession>
    <name>W5NL94_LEPOC</name>
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        <submittedName>
            <fullName evidence="7">FinTRIM family, member 98</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NL94"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021403"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021440.1">
        <property type="protein sequence ID" value="ENSLOCP00000021403.1"/>
        <property type="gene ID" value="ENSLOCG00000017298.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286950"/>
    <dbReference type="HOGENOM" id="CLU_013137_4_1_1"/>
    <dbReference type="InParanoid" id="W5NL94"/>
    <dbReference type="OMA" id="CIETHWI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017298">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="PANTHER" id="PTHR25465">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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            <begin position="15"/>
            <end position="57"/>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="199" mass="22581" checksum="C1E31FD1B91FA865" modified="2014-04-16" version="1">YSEFSAILLGEMCTCSLCKTLLKDPVSIPCGHSYCRQCIQGYWDRPVDAGSYPCPQCGKISDRLPILYPSTFLDQLLQKLQTPLLSSALHGHDCSRPGDVACDFCTGRKLKAVKQCLTCIVSYCEKHVWQHYTVPAMQCHRLVEVTGRLEFRLCQQHNRALEVFCKTDQTLICTLCAIQEHRSHDIVVAKTEQELKQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLF1</accession>
    <name>W5NLF1_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLF1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021460"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021497.1">
        <property type="protein sequence ID" value="ENSLOCP00000021460.1"/>
        <property type="gene ID" value="ENSLOCG00000017355.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1792">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177131"/>
    <dbReference type="HOGENOM" id="CLU_339770_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017355">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="45"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="778"/>
            <end position="794"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021460.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="836" mass="91295" checksum="7953DC3D4C2ACA1C" modified="2014-04-16" version="1">MQEQPCSTRPVDQKEGTSVLGTVAPVSSTSPLPSPDSLEELSLSESPNKALSLAAPFASCPVQVADAFAQIVVQDVPLNEEVKNFECKTQTDNMTLRLDAHQADNQVRLDDVSMKIVPDLDASQIVAPSLFPLQETLNLSQDTVLDHKEEASCTLETLLADSASLKTVVMEPEKPVFCKDSEVMFSSDIKLGDSKETYEEEKMDSDREERDSYPHTSEEAAGSTLPQLLPNTETSQGVGTQHFVTYATFDPAVNFDSPPSDSKDDSKVLDVPSEDDSALNASSFKVGLPTGQAEVGHEVLLELKTSAALDTSVQKESLRELSPIDVSSGGLPQYSETVSEDKAAEMSESPTPDLVQDAYDNDLQDLTPTKHQEETPVDLVQMISDSAYEPLDPSMHLPPSTKESEGSAAPPSLPDILKSSPLGSSKPEIVSSEGTPDSETSPDQEHLQEPRDVSPSIEAAKLTAFEMDSKIPHFRETAGQVAFEEAEKKDRAFEQVADKGLSTCDLVKEAQSTTDHKDQFRMEPDGKDWMNFTCPDAAQSLGSHESQNPEPPIDLTASHAPKESVPEESDSESPMTDSLSPVLEAMAKNPAIFQVEIERYQKDTETSTTPETQSYPESGTFLEEADESVELEEAFEQEGSSEEFEMIERPPVGSRGVIDEFLETLDNSKFAKTSEAGTDDEPSPGFGKDELVPDIDTEFHVRSQLNSYTILSEPTPGPLPQKVLLDLEAPAVCSEEPKMGQAQEIVPEDVHLHDPRVDSTVEETEKLVSPPPPPPPTAEDKPSSREKARLEAPKATHLPPLNTEKGNFQHARVHHPVWHEFPSPQGPLVIRLFFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM65</accession>
    <name>W5NM65_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NM65"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021724"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021761.1">
        <property type="protein sequence ID" value="ENSLOCP00000021724.1"/>
        <property type="gene ID" value="ENSLOCG00000017619.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014380"/>
    <dbReference type="HOGENOM" id="CLU_562100_0_0_1"/>
    <dbReference type="InParanoid" id="W5NM65"/>
    <dbReference type="OMA" id="WFRSVEV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017619">
        <property type="expression patterns" value="Expressed in intestine and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052090">
        <property type="entry name" value="Cytolytic_pore-forming_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048997">
        <property type="entry name" value="Stonustoxin-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040581">
        <property type="entry name" value="Thioredoxin_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594">
        <property type="entry name" value="AIG1-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594:SF16">
        <property type="entry name" value="SI:CH211-281L24.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21109">
        <property type="entry name" value="Stonustoxin_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18078">
        <property type="entry name" value="Thioredoxin_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="273"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="368"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="486" mass="54675" checksum="81DBA7E9D4C03C38" modified="2014-04-16" version="1">MPMKVWLYPLTKLDSQAAKIVRQISIGLVRRSQRILDEMDDYIVQCQDLMKDAIAIQFPEIKQKLLRFKDLCVEYKLVFQKSLARLLPAIRGGGEEEGRLIDLLSSRERSPFRNALLVQFLEDKERELNVLRYYLQKMSDVTVAPSSSDLDQVVLNSANEYVVCFAFTSLKHREQYLTILESYLTSFERGTGEEVAPDLDPEGRPGKWFRSVEVTALTRENIRLFLDFERENKGRRNVKFCVASIDDEVNIGSSVYLFERGALASKHFELPSQPGVPAVLTTLHDSVQLEFHPPKYGAREIVRYRVLYQKPGDSAWMEVDTDDNKLEFTVSGLDLNASYLFGCQAVCKPGVSCTSNASPVCKTFPSAPPGAPQQKSTGPNSMIITWDMPTSVGQGVSVTSYEVEYREVFKGATDDQDSKTWTKLKARGRVCTLENLTAGTSYIVRVYSNCGKEGKSPPSPETQITIPKDSTVKARSEEVSHVKVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMP8</accession>
    <name>W5NMP8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine protease 23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219649.1">
        <property type="nucleotide sequence ID" value="XM_015364163.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219650.1">
        <property type="nucleotide sequence ID" value="XM_015364164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMP8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021945.1">
        <property type="protein sequence ID" value="ENSLOCP00000021907.1"/>
        <property type="gene ID" value="ENSLOCG00000017803.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686087"/>
    <dbReference type="KEGG" id="loc:102686087"/>
    <dbReference type="eggNOG" id="ENOG502QTW6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000155"/>
    <dbReference type="HOGENOM" id="CLU_055829_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMP8"/>
    <dbReference type="OMA" id="WIKGNFM"/>
    <dbReference type="OrthoDB" id="10037376at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017803">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000040">
        <property type="entry name" value="Serine protease 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000048">
        <property type="entry name" value="serine protease 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF10">
        <property type="entry name" value="SERINE PROTEASE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868006" description="Serine protease 23" evidence="11">
        <location>
            <begin position="17"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="121"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="42650" checksum="CBFDD2DC8FFB9CBC" modified="2014-04-16" version="1">MEALPTLLLLLGSVQGAYHYSPFWKPSWPRAKLPVVLPQLTEDLGAPKFNADAKLEVLSPCGPECHKQAPIPSYEDLKEFLSYETLYFNGSLTETKVGIYGFHLADLAGPQGARGRSRAKRQIYGHDSRFSIFGKNFLLNYPFSTSVKISTGCTGTLVAEKHVLTAAHCIHDGKSYVKGAQKLKVGFLKPRQKETAESNKTGSDVPEKMKFQWIRVKRTHVPKGWIKGNANDIGMDYDYALLELKKTHKRRYMKIGVSPSARELPGRRIHFSGFDNDRPGNLVYRFCEVRDETYDLLYQHCDAQPGASGSGVYVRMWNRQHRRWERKIIGIFSGHQWVDRNGSPQEFNVAVRITPLKYAQICYWIKGNYVDCRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN56</accession>
    <name>W5NN56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01004835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN56"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022065"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022104.1">
        <property type="protein sequence ID" value="ENSLOCP00000022065.1"/>
        <property type="gene ID" value="ENSLOCG00000017962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165682"/>
    <dbReference type="HOGENOM" id="CLU_012949_3_2_1"/>
    <dbReference type="InParanoid" id="W5NN56"/>
    <dbReference type="OMA" id="IARESMF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017962">
        <property type="expression patterns" value="Expressed in embryo and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF63">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY, POLYPEPTIDE F1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="532" mass="60788" checksum="4527C1A78F4A1F8F" modified="2014-04-16" version="1">MQTCGSWKTLPTLISLLIIISVPFSDGGKILVFPVDGSHWVNMNILIEEMHSRGHNITVIRGEGSWYIKENSLYYTSITIPENKDMDNKFFHSFLVKYLEVQIKESSLLTFLTLKRDFLAMISEVHRAICKMVTTMFENKQLMQRLQDTQYDLVLTDPAIAGGVLLAHYLKLPLVLNVRWITSGEGHFALAPSPLSYIPVPGSRFSDQMNVIERVKNFLYYAIILYEQTFVVDPHYRAVCDHYFGPGIDIYSLIQSADIWLMRVDFVFEFPRPTMPNVVYMGGFQCKPAKPLSPDLEEFMQSSGKHGVIIMSLGTLVNSLPIDIANEIASAFAQLPQKVIWRHQGNRPANLGNNTLLVNWMSQNDLLGHPKTRVFVAHGGTNGVQEAIYHGIPVLGLPLLFDQYDNLLRLKGKGAAKILEINKVKSNTFLQALLEVLNDPSYQMNMKKLSSLHWDQPIRPLDNAAFWIEYVIRHKGAAHLHSESYKMPWYTYHSVDVIMFLLAAVFVFIMITIAVIKFICHRIYYRRKLKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNB9</accession>
    <name>W5NNB9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNB9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022128"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022169.1">
        <property type="protein sequence ID" value="ENSLOCP00000022128.1"/>
        <property type="gene ID" value="ENSLOCG00000018027.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVH7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162014"/>
    <dbReference type="HOGENOM" id="CLU_012526_1_0_1"/>
    <dbReference type="InParanoid" id="W5NNB9"/>
    <dbReference type="OMA" id="CNDISIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="56"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="327" mass="36692" checksum="414AD730F11783AE" modified="2014-04-16" version="1">VLQLQTDLAVKMIISNQTVTNISEFIVNGFSGIQDQRSQNILFAFFLITYVLTLFGNVSILIVIKFDKSLHTPMYTLIGTLAFLDLCIPSVTVPKILTNLMFRTKSIEFGACFTQMVFYLGVGSAESFLLMVMAYDRYVAVCDPLHYSTIMSNRHLIKLHILCWVGGMMVPMLPLYLAVRLPFCGPNVVLHAFCDYSAVVRLACANVTLNAIVSMTIAMSVLLIPLAFIFFSYIKIIISVIKVASSEGRKKAFSTCASHLLVILVFYLVAVFVFITYRIQGFSDDVRIMAAVLQNVIPPLVNPIIYCLKTRDIRKSFLKFIRKQVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNF8</accession>
    <name>W5NNF8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Potassium channel tetramerization domain containing 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KCTD8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01026331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNF8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022167"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022208.1">
        <property type="protein sequence ID" value="ENSLOCP00000022167.1"/>
        <property type="gene ID" value="ENSLOCG00000018066.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161041"/>
    <dbReference type="HOGENOM" id="CLU_057051_0_1_1"/>
    <dbReference type="InParanoid" id="W5NNF8"/>
    <dbReference type="OMA" id="ISEMCTS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18396">
        <property type="entry name" value="BTB_POZ_KCTD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22218">
        <property type="entry name" value="H1_KCTD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000031">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049904">
        <property type="entry name" value="H1_KCTD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057093">
        <property type="entry name" value="H1_KCTD8_12_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF68">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23110">
        <property type="entry name" value="H1_KCTD8_12_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="31279" checksum="249C0C12603DD203" modified="2014-04-16" version="1">PREMALKENILPISEMSSPFPAVVELNVGGQVYVTKHSTLVSIPDTILHSMFTKKQVKDLPRDNRGRFFIDRDGFLFRYVLDFLRDKQLVLPDHFPEKERLLREAEHFQLNDLLKLLTPKVTKQSSMNDEGCQSDIEENSQSSDLARTMALDKKSGFITIGYRGSYTMVRDNQTDAKFRRVARIMVCGRIALAKEVFGETLNESRDPDRLPEKYTSRFYLKFTYLEQAFDRLSEAGFHMVACNSTGTAAFINKYRDDKIWSSYTEYIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNT9</accession>
    <name>W5NNT9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 181 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015197075.1">
        <property type="nucleotide sequence ID" value="XM_015341589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNT9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022298"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022339.1">
        <property type="protein sequence ID" value="ENSLOCP00000022298.1"/>
        <property type="gene ID" value="ENSLOCG00000018197.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695517"/>
    <dbReference type="KEGG" id="loc:102695517"/>
    <dbReference type="eggNOG" id="ENOG502QS8E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154730"/>
    <dbReference type="HOGENOM" id="CLU_055561_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNT9"/>
    <dbReference type="OMA" id="PNFFTDC"/>
    <dbReference type="OrthoDB" id="5981837at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018197">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029359">
        <property type="entry name" value="FAM181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053819">
        <property type="entry name" value="TEADIR3_omega_loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33766">
        <property type="entry name" value="PROTEIN FAM181B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33766:SF2">
        <property type="entry name" value="PROTEIN FAM181B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15238">
        <property type="entry name" value="TEADIR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="326" mass="36076" checksum="D2102D4B7B5033DF" modified="2014-04-16" version="1">MAVQAAIMTPHFMHFCFPGSVMEYEMDKSFDGGVLGEMDCEGDFKETTRDLLSFIDSASSNIKLALDKPVKSRRKVNHRKYLQKQIKRCTGMITPGSGGQEAVKRQGSPQSNPGTSFQCKPPPKREGIQSNLQSKSLAALFDSVKDIRAEKGKKLPLRHRNLPRSFFTEPANSSKVTSTSGMTLKDLERGNPEAAEFFELLGPDYSNMIADHDAFQGVPVRIQQEAALEPGSYDPQHLVGGFLYTEPWSTCSSPSKKTGICSLNVNENMRTIPVQPPVYCHSDSAVTSPQEENASGMPMFAHFFTDCSLPQYDYSSGYNRTNFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWG4</accession>
    <name>W5LWG4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small glutamine-rich tetratricopeptide repeat-containing protein beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-SGT</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homooligomerize.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639838.1">
        <property type="nucleotide sequence ID" value="XM_006639775.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221164.1">
        <property type="nucleotide sequence ID" value="XM_015365678.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221165.1">
        <property type="nucleotide sequence ID" value="XM_015365679.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LWG4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000471.1">
        <property type="protein sequence ID" value="ENSLOCP00000000471.1"/>
        <property type="gene ID" value="ENSLOCG00000000394.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684959"/>
    <dbReference type="KEGG" id="loc:102684959"/>
    <dbReference type="CTD" id="6449"/>
    <dbReference type="GeneTree" id="ENSGT00940000159037"/>
    <dbReference type="HOGENOM" id="CLU_044224_0_0_1"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000394">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000002">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000103">
        <property type="entry name" value="small glutamine-rich tetratricopeptide repeat-containing protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032374">
        <property type="entry name" value="SGTA_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF3">
        <property type="entry name" value="SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16546">
        <property type="entry name" value="SGTA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SGTA homodimerisation" evidence="13">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="278"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="313" mass="33805" checksum="A8C2EC002FFB90A7" modified="2014-04-16" version="1">MADTKRIAFSIIQFLHDQLNSGDLSSDAQESLEVAIQCLETAFGVSLEDQSLAVMQTLPELFVSATNKPETPQVQINAVPDTPTEEDIAEAERLKTDGNDQMKAENFGPAVEFYSKAIELNPRNAVYFCNRAAAYSKLGNYAGAVQDCEQAITIDPNYSKAYGRMGLALASLNKHSEAVSYYKKALELDPENDTYKSNLKIAEQKMREMPSPTGGMGGADLAGLLSNPGFMNMASSLMNNPQVQQLMSGMMSGAYGPMGATAGGGPGPNDLSGLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNEEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWX2</accession>
    <name>W5LWX2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWX2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000629"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000631.1">
        <property type="protein sequence ID" value="ENSLOCP00000000629.1"/>
        <property type="gene ID" value="ENSLOCG00000000570.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RTUS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1380926_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWX2"/>
    <dbReference type="OMA" id="SKIACAD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000570">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22273" checksum="1F57832197F035E7" modified="2014-04-16" version="1">SLKKTDKPVSTVQEPSSDSGKETVQINGQEIKMVIDTGSGRNLIGVERFKKVFAHRTKLKKTNKKCYAYAQISPLQCVGYFEAQLKWQENVIKDKAYGIEGNVEQLLRRKACFDLKILNVGDTVNVVDQSSERFQQLAQEYESLFKGLGQIKVTVDEKVLPVAQSLRRVPYPVIEAVNQELDKILDDGIIEQVNQGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXF7</accession>
    <name>W5LXF7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine protease 27-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LXF7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000814"/>
    <dbReference type="MEROPS" id="S01.028"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000818.1">
        <property type="protein sequence ID" value="ENSLOCP00000000814.1"/>
        <property type="gene ID" value="ENSLOCG00000000732.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155138"/>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="InParanoid" id="W5LXF7"/>
    <dbReference type="OMA" id="HDPESCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000732">
        <property type="expression patterns" value="Expressed in bone element and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF127">
        <property type="entry name" value="SERINE PROTEASE 27-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="81"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="315" mass="35011" checksum="6C38CE8CDDA2BABD" modified="2014-04-16" version="1">MRSQEVLNVKNLSSEREPENPEICTVALSLFTGEHQLLRSSALTRTEILKRMKMVFLRRLFGVMILSSFEVSSSPLSRSSIVGGEDAGEGIWPWQVFLKMKNTDGRNGSCGGSLISDVWVLTAAHCLDSLFYPTSSVVYLGAYQLDKAGSHQVNRTIADVVIHSKYTIPTKGYDIALVKLDRPVPLSRFIQTVPLAEPSDLFTSRSDCWVTGWGRVQQNSRLPFPRTLQKVKIPIHDPESCKNVYPNMKTSMICCGGQYKSTCKGDSGGALVCKKGNRWVQAGIVSYGKGCETPIPPKIFTRVSSYRSWIKIHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYE6</accession>
    <name>W5LYE6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LYE6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001153"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001157.1">
        <property type="protein sequence ID" value="ENSLOCP00000001153.1"/>
        <property type="gene ID" value="ENSLOCG00000001029.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSRZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271914"/>
    <dbReference type="HOGENOM" id="CLU_013137_8_4_1"/>
    <dbReference type="InParanoid" id="W5LYE6"/>
    <dbReference type="OMA" id="HEDETYR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001029">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002490">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF130">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="130"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="23541" checksum="39D4B3B12D24EB9A" modified="2014-04-16" version="1">TFQVVGPAAPVVVSPGEDAVLPCYLSPRENAEDLQIRWFRDDYAAPVCLYQNRRYIFEDQIPSYRGRVELFPGKLFKGNVSLRLRDVRRSDHGLYNCLVRFPRYYEVAVIDLGVRALGSQPSVSLHSPGGDQAQLLCRSEGWFPQPAVIWTDRDGNEVTSQPPTVDTDSQGLLSVSSSLSKHTGVRTGSVVCAVNTQEVFQETISLLPLAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZU7</accession>
    <name>W5LZU7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phospholipase D3-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZU7"/>
    <dbReference type="FunCoup" id="W5LZU7">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001654"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001659.1">
        <property type="protein sequence ID" value="ENSLOCP00000001654.1"/>
        <property type="gene ID" value="ENSLOCG00000001445.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685022"/>
    <dbReference type="CTD" id="558442"/>
    <dbReference type="eggNOG" id="KOG3603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183059"/>
    <dbReference type="HOGENOM" id="CLU_332868_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZU7"/>
    <dbReference type="OrthoDB" id="1923775at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001445">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032803">
        <property type="entry name" value="PLDc_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF26">
        <property type="entry name" value="PHOSPHOLIPASE D FAMILY, MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13918">
        <property type="entry name" value="PLDc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="774"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="859" mass="92933" checksum="75A60DB37CE9EB17" modified="2014-04-16" version="1">MGRRRSARLARAETPLRRSARLRDTLGGPVEEVVDMSAEQSEGEDAQDLQPRPGIRAHHSASGDPAPVEPEPESELEEPEARGRGRPRQHPQLDSMCPSVILRRLDLEDREGEETEPPRAAAPKAPTSRIPTFQSSGARLLRPVDRPPLGKPPPSQLPVRPWERAAKPHLELGWSDTALSPRPALRAETGTPRPEAAQEAGAGALAPETRGGQLERGTPAPRAEATSREASKTQPRRGPPVPSEAKPREDTPPASEEGGEEAEAEREEVGSEVGSEAEREEAGSEAEEKEAGEEEVDSEAEEKEVGSEAGEEEAEQEEAAPEAEAEGEEVGSEAEAEAEREAVVFEVGARSEKPALNPRGRRAAGGREARQEGAGRPGPRGRLRRYCSTLLCLLPPLLLLLGGGGLFLWPSGRPGLAGALLAPLDLDWLWAGDLWARQEACGSDCSLSLLESLPEGLEYPPGSPRLTPISQAWRDLLHRANHSVSIAAFYLTLRDTDLEASEPSALEGKRVFEALRGLPARGVGLRIAVNSPQTYPRDTDDLALAGAEVRPVYLQNLTGGILHTKLWEVDGQHAYLGSANMDWRSLTQVKELAVSVTNCSCLARDVSRLFQAYWLLGSPAGGAVPARWPRRLSALSSAQRPLQLQLNGLPAEVYLSSAPPPLSADGRTDDLSAILGVISDARRFVYVSVMDFLPASRYTEPARFWPPIDSALRAAACARGVEVRLLVSCWPHSDQPMFVFLESLGVLNHPPLSCTLHVKVFEVPSTKEQKMIPYARVNHAKYMVTDRVAYIGTSNWSENYFTHTAGVGLVVNQTGAPAGAGKRSVQSDLQAVFERDWDSGFAQPLSSEHAHHCGARESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M060</accession>
    <name>W5M060_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger MYND-type containing 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M060"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001767"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001772.1">
        <property type="protein sequence ID" value="ENSLOCP00000001767.1"/>
        <property type="gene ID" value="ENSLOCG00000001538.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690774"/>
    <dbReference type="KEGG" id="loc:102690774"/>
    <dbReference type="CTD" id="84217"/>
    <dbReference type="eggNOG" id="ENOG502QSY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004248"/>
    <dbReference type="HOGENOM" id="CLU_058444_1_1_1"/>
    <dbReference type="InParanoid" id="W5M060"/>
    <dbReference type="OMA" id="KILFMLY"/>
    <dbReference type="OrthoDB" id="3174329at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001538">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053248">
        <property type="entry name" value="Zinc_finger_MYND_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533:SF1">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="356" mass="39702" checksum="7988A50635091BB4" modified="2014-04-16" version="1">MSEITPLANPRGVKRLCELCQRPARLQCSHCRVTYYCDSDHQGADWVGIHEKVCQLLIPLRTAVPFHTLEVEREHHRAQTLQRQEQLIELSRATAQKKLFEGKHQEAVPAALLSLRCCVEIHGTSALQLVPSYLLLAEACTGLGNLTQAEEYLCRAEWTVLKNTECGRRLRHTLHRSLGLLHAAKGDLEGALEHLASDVYYASEEFGSDHIVTSGGYFHMANVFFRQNKMDIADSLYTEVTAIWHRHLSSLLEAHQRSASAPADPPLEHSLSEAQQAEAQQVLGAILDIREQAPRPDPTQTARAAHSLALLHFLGTNTPKVVEFGRKALRCSQLVPDCGLTEPIERLLQLAESRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1D5</accession>
    <name>W5M1D5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ladybird homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="71" mass="8196" checksum="EDBB48247B3388E1" modified="2014-04-16" version="1">MSVVFVPKKQRGKAQINHEIIQRLLDENDQLVRCITEYQQKGRATECVQYQQILHRNLVYLGNIADASPSV</sequence>
</entry>
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        <submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
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                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006639953.3"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000002761.1">
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        <property type="gene ID" value="ENSLOCG00000002346.1"/>
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        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="HAD_FCP1-like"/>
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    </dbReference>
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        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dullard_phosphatase"/>
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        <property type="entry name" value="FCP1_dom"/>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="TIM50"/>
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        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="183"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50969"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002755.1"/>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="366" mass="42475" checksum="1D508827888CF708" modified="2014-04-16" version="1">MLLRSRRIPARPPSEPSTPRSLRPRSPSRRAPPSAKAGGRAAVRGEVGLDSLQDPEEEEVHVLKPLTMQTRSKGKVSKTDSEDFKTPVKGQQLRVRFEMDESSCKNTAVRNICSPLVHFLLPGQDDEKSPQPEQEVFDYSRFNPVLKDEDNCKECDLYTFIKSIPLQSQHSKVRIRDIPVKTRSTPEATLVLDLDETLVQSSLGVIPNAEYTFLARFQDQEYKVYMKVRPYVQEFLENMTKIFEIFVYTSAKKEYAEKILDILDHQKRLIRHRLYQEDCLCIMGHYIKDLTILNRDLAKTVIVGNDPYTFPYHLMNMIPIQRWTGNKDDKALPRLAPYLEKLSDTRDFRRVLKRRTDNFRGLLSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3T3</accession>
    <name>W5M3T3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ferric-chelate reductase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01037013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3T3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003041"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003047.1">
        <property type="protein sequence ID" value="ENSLOCP00000003041.1"/>
        <property type="gene ID" value="ENSLOCG00000002589.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SCMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000051759"/>
    <dbReference type="HOGENOM" id="CLU_1528602_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3T3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002589">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900449">
        <property type="term" value="P:regulation of glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042789">
        <property type="entry name" value="FRRS1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902:SF1">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867564" evidence="1">
        <location>
            <begin position="21"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="176" mass="18160" checksum="620726BCEC58EF3A" modified="2014-04-16" version="1">MDQRLSVALLLLGLTVLVDGQTVAAPFTSPQGNITRNGCGATKFCIETPTGCDPASGGNCLFASAARDTTSTTGISFNLQGNSSGYIAAILSTADNNSMGDAFVCAQNASDTLFYRALYSSNGSSLSPQILNNAVGNITGTTMNNVIKCAFIVRNLTRSRALAQYSISLAVGSLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M409</accession>
    <name>W5M409_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3 13">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">PPIases accelerate the folding of proteins during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3 13"/>
    <dbReference type="EMBL" id="AHAT01012250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212548.1">
        <property type="nucleotide sequence ID" value="XM_015357062.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M409"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003124.1">
        <property type="protein sequence ID" value="ENSLOCP00000003117.1"/>
        <property type="gene ID" value="ENSLOCG00000002630.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683944"/>
    <dbReference type="KEGG" id="loc:102683944"/>
    <dbReference type="CTD" id="11328"/>
    <dbReference type="GeneTree" id="ENSGT00940000157125"/>
    <dbReference type="HOGENOM" id="CLU_034907_0_0_1"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002630">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000002">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051989">
        <property type="entry name" value="FKBP-like_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867235" description="peptidylprolyl isomerase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="15">
        <location>
            <begin position="48"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="15">
        <location>
            <begin position="160"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="15">
        <location>
            <begin position="272"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="15">
        <location>
            <begin position="384"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="483"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="528"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="566" mass="63256" checksum="803BA7B7FE050B06" modified="2014-04-16" version="1">MSYGLQSMFFLALLIAFAACNAPPVPLDDVVIEKTFVPDECVRAVKPGDFVRYHYHGTFPDNKKFDSSYDRGSTYNVYVGKKQLIEGMDKGLVGACVNERRLIKIPPQMAYGKEGYGDVIPPDSILHFDVLMVDIWNPEDKIQIQTYYKPENCSRTVQVSDFVRYHYNGTLLDGTLFDSSHTRLHTYDTYVGIGWLIAGMDQGLLGMCVGEKRVITVPPFLGYGENGDGSDIPGQASLVFDVVLLDFHNPKDSIAVENQVVPESCPRKSKSGDFMRYHYNGTLLDGTLFDSSYSRNRTYDTYIGKGYVIAGMDEGLLGVCIGERRRIIIPPHLAYGEEGTGTKIPGSAVLVFDVHIIDFHNPSDSVEIKTLFKPDDCKVQSKKGDFVKYHYNASLMDGTFIDSTHSYGKTYNVVLGSGQVVLGMEMGLEDMCVGEKRTLVIPPHLGYGERGVEGEVPGSAVLVFDIEMVDMEEGLPEGYMFIWNSEVSDDLFTEMDKNKDGHVDSTEFSDYIHRQVSTGKGRLAPGYDAYRIIDNMFSNQDRNGDGKITAEEFKLKDQETKEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M516</accession>
    <name>W5M516_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG20"/>
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        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040006">
        <property type="entry name" value="TNKS1BP1-like"/>
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        <property type="entry name" value="RIKEN CDNA 2900026A02 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22042">
        <property type="entry name" value="TANKYRASE 1 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="586"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="633"/>
        </location>
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        <location>
            <begin position="684"/>
            <end position="777"/>
        </location>
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        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
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        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
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        <location>
            <begin position="168"/>
            <end position="177"/>
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            <begin position="200"/>
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            <begin position="233"/>
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            <begin position="325"/>
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    </feature>
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            <begin position="359"/>
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    </feature>
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        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="777"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="777" mass="81571" checksum="09780B2782A87310" modified="2014-04-16" version="1">MAAEVGMKSGPPSLGERAGGDGRPGGGAARAHLSPLTDSSNKVPPEPTSRSQGSLILPAEPAKPLPGPKPRLTPKPFTVDRPSAIRPILAPKPSAKSEARPAPANTSKPSLGGVTGPSAKPSPNTPKPISTTPNATPEVPTGSPSFKPSPTPKPDQSPGSAAPCTPPASVQPQTVTVIGTADTPGAGQGLRGCQSAEWAGRTRAEAEPEEGGRARRAGSSITRAKSMGFLNRAESRGAEDRQQEGQNRAPGPDTPSQLRSRAKPSKPRPVSAMYPSSSNQPDASPTKDPGTRPWVAARRPLSSDLTAKFESIGLSLHSKAGRAVHSKENKPDSAKEEEEEEEEEEEEENAPAPARRRERVLERGAEGVSPRDLERETAAPETKPVPEEAEPGEEGKEETLPPTGGSIKRRISQLLDSSSSPSSGGGVNTQGSAPPLADVEPTVGVKQRIKELTATDTPPGRAEPQRRSIKPRPLPADLTRRFARENPAPSGNEATRSRSESDSDAHRKVDDLPPSPLGDPERGWKGEEGTVAEGGAHEPCALAAPPAATTPRRREEEDEEKVTEGRRESGNGGAPSPNCPSSPGVLTVRASLFDNVVERHTVGEARPLAEGGRGGEHAQRSQSVRYGVPAQDGARQRGILATASFRETPTFSTAVVPGPLAALTKVEHVYDAVGAVGERRAVSEELPAAQHEDRALTLRSRRASSGEPATPPAQGSRCLRVGALQKWRTDAPLQAGGVQSSPDSPGAEWHSRRAPGVPAELEQKEGELEKEREGELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5K1</accession>
    <name>W5M5K1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Immunoglobulin superfamily member 21</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in synaptic inhibition in the brain. Selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic NRXN2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (Ig-like 1 domain) with NRXN2 (via Laminin G-like 1 domain) in a trans-interaction manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Postsynaptic cell membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
            <topology evidence="13">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5K1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003659"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003666.1">
        <property type="protein sequence ID" value="ENSLOCP00000003659.1"/>
        <property type="gene ID" value="ENSLOCG00000003096.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQMY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002421"/>
    <dbReference type="HOGENOM" id="CLU_054054_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5K1"/>
    <dbReference type="OMA" id="YTEHPSR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003096">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099550">
        <property type="term" value="P:trans-synaptic signaling, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000509">
        <property type="entry name" value="Immunoglobin superfamily member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000858">
        <property type="entry name" value="Immunoglobin superfamily member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF121">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="62"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="371"/>
            <end position="456"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="516" mass="57142" checksum="771BC713681EFC40" modified="2014-04-16" version="1">MVGDTQPRNGEPLKTDGVWDLLQNKGKMTVFSLCFLLCADLLDLAVGYLTVTIESLPPVVVGDTVTLKCNFKTDGRLREIVWYRVTDGGTIKQKIFTYDAMFNTNYSHMEDYRRREDLVYQSTVRLPEVRISDNGPYECHVGIYDRATREKVVLASGNVFLTVMSPPLSISVVAADTPAPFSRYQAQNFTLVCIVSGGKPAPTVYFKRDGELIEVIPYIQPTAGGEGGAAGLKDSRPPRPLISRELDDTKMQKSLSLLDLDGRSARLFTENPLRGHTQGAPGFEPSPATEIIPETVVSREFPRWVQSSDPLYYFRHVHIPLNDGTVEVRAMITWTLNPQLDNDALFSCEVKHPALSMPMQAEITLAAPKGPKLLMSPTRAKVGDTVRITVQGFQNEVFPEPLFTWTRVGGRLLDGSLEHDGKELVLERVPAELNGSMYRCTAQNPLGSTDTHTRLIVFENPSMMKGTQKLNNAAPCMETLGLTTAVLILTVTLELTLQYTTSAEILLEQVENCAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M650</accession>
    <name>W5M650_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flotillin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Heterooligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M650"/>
    <dbReference type="FunCoup" id="W5M650">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003858"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003866.1">
        <property type="protein sequence ID" value="ENSLOCP00000003858.1"/>
        <property type="gene ID" value="ENSLOCG00000003267.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00560000077232"/>
    <dbReference type="InParanoid" id="W5M650"/>
    <dbReference type="OMA" id="CHARPCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003267">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000003">
        <property type="entry name" value="Flotillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031905">
        <property type="entry name" value="Flotillin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15975">
        <property type="entry name" value="Flot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0967">Endosome</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="6">
        <location>
            <begin position="114"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="243"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="455" mass="50212" checksum="2E1027F03EE7A201" modified="2014-04-16" version="1">MGNCHTVGPNEALVVSGGCCGSDEKTYMVGGWAWAWWFISDTQRSALEQSCSLNLQSSLVKKMQMFYVFHRITLEIMTLQPKCEDVETAEGVAITVTGVAQVKIMTENELLAVACEQFLGKSVQEIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVEYLSSLGKTQTAAVQRDADIGVAEAERDAGIREADCKKEMMDIKFQADTKMADSKRELEMQKAAFNQEVNTKKAEAQLAYELQAAKEQQQIRQEEIEIEVVQRKKQITIEEKEVARMDKELIGTVKRPAEAEAYKMQQLAEGQKIKRVLTAQAEAEKIRKIGEAEAGSIEAIGKAEAEKMRLKAEAYQQYGEAAKTALVLEALPKIAGKVAAPLAKTNEIVILSGDGSRVTGEVTRLLAELPVSVNALTGVDLTKIPLLQKITNPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6E2</accession>
    <name>W5M6E2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein X-linked</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6E2"/>
    <dbReference type="FunCoup" id="W5M6E2">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003950"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003958.1">
        <property type="protein sequence ID" value="ENSLOCP00000003950.1"/>
        <property type="gene ID" value="ENSLOCG00000003347.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164731"/>
    <dbReference type="HOGENOM" id="CLU_021097_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6E2"/>
    <dbReference type="OMA" id="NDESEAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003347">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000054">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000209">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000170">
        <property type="entry name" value="Zinc finger protein 711 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002525">
        <property type="entry name" value="Zinc finger protein Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000461">
        <property type="entry name" value="Zinc finger X-chromosomal protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006794">
        <property type="entry name" value="Transcrp_activ_Zfx/Zfy-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04704">
        <property type="entry name" value="Zfx_Zfy_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="591"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="706"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="735"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="675"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="765" mass="84603" checksum="2B38A70E99D29632" modified="2014-04-16" version="1">MDEDVTGLALHPQEPKIFFDGSDADAEMQAAAHADEGEIVVEVQETVFVSEAGGAGVAVHGFSPEELDPVVIQDAIENVVAEYVQCSEAEGPGDVLIPDEMLGSDAPAGVSAEACVIPAGGLSEAGGVVLHAGTALADIPVEALQVDVVPEEVLVSECDPAGAIDAEQDRTTCGDYLMISLDEADKIVQHGSGEIAVDAGAEEQTPSPKEDGGEVIKVYIFKAESGEEDLGGTVDIEESDAEHEHGVGLLEPSGHGPREKMLYVTVGGSQPDEEDLNAAEVGGEVYMEVVVGGEEAMAHEQQYDNTVLSKDFMPVAWAAAYGNDESEACENRNGAASALLHIDESASAGKGAKQKTKRRKKIDARQFQTAIIIGPYGQPLTVYPCVLCGKKFKSRGFLRRHMRNHHEVLNKKKYQCTDCDFTTNKKASLHNHMEVHKLTGKAEKAVECEDCGKEFSHAGALYTHKMVHRDKGATKMHRCKFCDYETAEQGLLNRHLLAVHSKNFPHICVECGKGFRHPSELKKHMRTHTGEKPYQCLYCEYKSADSSNLKTHVKTKHSKEMPYKCERCFQTFPEAEELLHHALSHEEAKTHQCAHCDHKSSNSSDLKRHVISVHTKDYPHKCDVCGKGFHRPSELKKHMAAHRSKKVHQCRHCSFKISDPFVLSRHILSVHTKDQAYPGRRRRKPFGPRGELKKAGGKAHRERRVYQCQYCDYSTGDASGFKRHVISIHTKDYPHRCEYCTKGFRRPSEKNQHIMRHHKDMVPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6V9</accession>
    <name>W5M6V9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA thioesterase 9, tandem duplicate 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl coenzyme A hydrolase family.</text>
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        <property type="gene ID" value="ENSLOCG00000003460.1"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <sequence length="434" mass="48950" checksum="C3CF3128E6C7FC2D" modified="2014-04-16" version="1">FFRLRLLNAASAVAARALSSGSAGSQGRSPDMAEGHSHLLVNDAVRNRLRQIVGASTNWSDHQQALRERASLTSQLATCQEDLPARKMKDSYIEVHLPLGSQPELREKYLNVHNSVRFGRILEDLDSLAVLICYSHTKRDTPQRSPLSIVTALVDKIDMRKNIIYPDCDIKFTGHVTWVGKTSIEAKMHMSQYHDGAYSPVLDATFVMVARDPENKRAAFVNPLQPDGPEEEKLFQQGEINKARRVELSTASLLKVAPTAEERKIVHSMFLNTLDPKTVSFRSRVLPANSVWMEDGKLKGLEICHPQERNIFNRIFGGFLMRKAYELGWANACSYGGSRPHLVAVDDILFQKPVELGSLLLLSSQVCYTEEKYIQVRVHTEVFDALTRQHNTTNVFHFTFCSQEEVPAFPKTYGESMLYLDGKRHFDVTMKGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M731</accession>
    <name>W5M731_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="HOGENOM" id="CLU_008078_2_0_1"/>
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    <dbReference type="OMA" id="RHYSKAG"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
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        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003545">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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    </dbReference>
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        <property type="term" value="P:regulation of synaptic plasticity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Junctophilin"/>
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        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
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        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF7">
        <property type="entry name" value="JUNCTOPHILIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MORN"/>
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        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="421"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="639"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="767" mass="83381" checksum="5D97F2A663BEC219" modified="2014-04-16" version="1">MSSGGRFNFDDGGSYCGGWEDGKAHGHGICTGPKGQGEYSGSWSHGFEVVGVYTWPSGNTYQGTWAQGKRHGIGVESKGKWVYKGEWTHGFKGRYGIRESTGSNAKYEGTWSNGLQDGYGTETYSDGGTYQGQWVGGMRHGYGVRQSVPYGMAAVIRSPLRTSINSLRSEHSNGTALHPDGAGASAAGSPAVSRGGFVLSMHSDAELLKGKKKGLFRRSLLSGLKLRKSESKSSLASQRSKQSSFRSEAGMSTVSSAASDINSTISLGEGEADLSVIEDDVDATTTETYVGEWKNDKRAGFGVSRRSDGLHYEGEWVGNKRHGYGCTTFPDGTKEEGKYKQNVLVSGKRKNLIPLRASKIREKVDRAVEAAERACDIAKQKAEIALSRTAHARVKAEAAVSAAQKAQEEARLARITAKEFSPSFQHRGNGVECQRPKHQNSSENDIEVISTGTPDSPELYMKGTTPPDLTPDLSPVPSRPSTPPPSPHKPTHRTKNARFLRQSAVDDDRGTGEIQVLLEGRGGGGGGGEYLRPNSWIEDKRPAEGGGGSRGGSRGSSRSNTPSTQEDTRARGSNGHKHASSNHKPRERWADCPTTVSWTTHRSLSESLANSRLLEQDEEKLSNYEMEMKPLKRMDSYMQETHPQKHYGSRGSPSPAEDYHAEARPHGHGLQRLRPKSQAKENFRLACTAEPAVQKLDSLGMGEKLDSRPLWRDLTLSPPQKSLPIALEHDEENLTELKSNSGSSSILVVMVILLNIGVAILFIHFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M800</accession>
    <name>W5M800_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M800"/>
    <dbReference type="FunCoup" id="W5M800">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004508"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004516.1">
        <property type="protein sequence ID" value="ENSLOCP00000004508.1"/>
        <property type="gene ID" value="ENSLOCG00000003779.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154827"/>
    <dbReference type="HOGENOM" id="CLU_062642_1_0_1"/>
    <dbReference type="InParanoid" id="W5M800"/>
    <dbReference type="OMA" id="TGCKHAS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003779">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20526">
        <property type="entry name" value="CYCLIN_CCNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000006">
        <property type="entry name" value="Cyclin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000052">
        <property type="entry name" value="cyclin-I isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="52"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="250"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="355" mass="40022" checksum="9B5E7EBBCDB8E04A" modified="2014-04-16" version="1">MKFSEPLESQRLSFLLEMATSREAAMWKVYVPKNPTNQDTDISPAQRDEVVRWLTDLHRRLQLYPGTLSLGISTLDRFLAIVKARPKYLRCIAISCYFLAAKTTEEDERIPALRHLAKNSCCGCSPSEILRMERIVLDKLSWDLHTATPLDFLHIFHAMVLSSRSQISAIVPGMNPSQHVYLLTRQLHHCLADHTLLQFKGSTLALAMVTLELEKCCPDWFALTADLLKKLQMDSSQLIRCRELVARRLSPHQAPTPPNTVYIYQPPPPPAPRDPRSTSRPEGAPTRPRPLIRPGWKPASAKRKVEEMEVDEFYDGIKRLYNEDSAPEGGGRRPSSRPRAGGPTAVRGGIPPGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M871</accession>
    <name>W5M871_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prostaglandin F2-alpha receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Prostanoid FP receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M871"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004579"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004587.1">
        <property type="protein sequence ID" value="ENSLOCP00000004579.1"/>
        <property type="gene ID" value="ENSLOCG00000003835.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234559"/>
    <dbReference type="HOGENOM" id="CLU_045991_3_1_1"/>
    <dbReference type="InParanoid" id="W5M871"/>
    <dbReference type="OMA" id="ILGHRNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003835">
        <property type="expression patterns" value="Expressed in muscle tissue and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004958">
        <property type="term" value="F:prostaglandin F receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000129">
        <property type="entry name" value="Prostaglandin F2-alpha receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000141">
        <property type="entry name" value="PglndnF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF4">
        <property type="entry name" value="PROSTAGLANDIN F2-ALPHA RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00855">
        <property type="entry name" value="PRSTNOIDFPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="43"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="373" mass="41388" checksum="25C3BEEECA8C224D" modified="2014-04-16" version="1">TAMSGNITVTLGLSAVNSSNTTRTRVELSVIVSIISMTVGILSNSLALFILVKAYRRFKLKSKASFLLFATGLVVTDFLGHLINGSIVLHVYCLHKEWERFDPQKVLCGFFGASMVFFGLCPLLLGSVMAVERCVGVTMPIFHSTKVTSRHMKKLMAVIWLSAMLVALLPIVAGKPYEVQKSGSWCFFKMGKSDWLDVFFPMLFSVLGLLSLAVSILCNTVTGVTLIRSKMKNQGHRKGTSHHFEMICQLLAIMLVSCICWGPFLVNVIIGSTQTQDDRSYERMLLAVRMATWNQILDPWVYILLRKAVLKKLFLITKECCGHKVISIYKWNCSSIKGSMKDSSISSRPDFSVSSSVRKSTPNTVTHCSAPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M872</accession>
    <name>W5M872_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentraxin (PTX) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M872"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004588.1">
        <property type="protein sequence ID" value="ENSLOCP00000004580.1"/>
        <property type="gene ID" value="ENSLOCG00000003837.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234935"/>
    <dbReference type="HOGENOM" id="CLU_714848_0_0_1"/>
    <dbReference type="InParanoid" id="W5M872"/>
    <dbReference type="OMA" id="DSEQWIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003837">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865693" description="Pentraxin (PTX) domain-containing protein" evidence="2">
        <location>
            <begin position="37"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="3">
        <location>
            <begin position="39"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="40469" checksum="D8314B015F2269B1" modified="2014-04-16" version="1">MLSGRKMLLWHLSDKKALGCPLFILLLLCNTPEGDGYFLGGSKAVFPSGCSQTWTIAEDVHVPQLTQLTVCVDIRLRASGDWTAFTYSTTQSQSQDLAITGDKKNVYVWLLGVQFPFPVNLSLNNWYHVCLKLNGQKQSISLDINGSPSVLKVNTTVVPPGGALVLGCKDSSTQRPSQNPGQAELYLFRVWSDINSHGTCEDGNVIGWDSEQWIFSKETLVPDASLTCSLTSTTVPPVANPSQGYKRVRRQASGSTSTAPSANTPSTTTQAVSGSGATVYSAATPSAPAVSNSTTTATNAAGTVSNTTDNPAEPLSTPAVSTAAAHSTSITTTASETTVFNSTTSTRASSTTAISPESPATQNMSDAVTPASNTTSTVSNTTASPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M988</accession>
    <name>W5M988_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Premelanosome protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PMEL/NMB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M988"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004947"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004955.1">
        <property type="protein sequence ID" value="ENSLOCP00000004947.1"/>
        <property type="gene ID" value="ENSLOCG00000004129.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV5K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183188"/>
    <dbReference type="HOGENOM" id="CLU_017264_0_0_1"/>
    <dbReference type="InParanoid" id="W5M988"/>
    <dbReference type="OMA" id="RDQDWLG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004129">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045219">
        <property type="entry name" value="PKAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046846">
        <property type="entry name" value="PKAT_KLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059017">
        <property type="entry name" value="PMEL_NMB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861:SF1">
        <property type="entry name" value="MELANOCYTE PROTEIN PMEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861">
        <property type="entry name" value="MELANOCYTE PROTEIN PMEL 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20433">
        <property type="entry name" value="PKAT_KLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26141">
        <property type="entry name" value="PMEL_NMB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="6">
        <location>
            <begin position="248"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="675" mass="70957" checksum="55D1127D97990348" modified="2025-10-08" version="2">MSTLQILLTGAFRDSTSSRSVHPRKGFIRYRSWNSRMYPMWKQGDHHSRNCWTGGDVTFNIINDSPTLTGAKVSFNIEIRFPGNQTVLPDGQVVWAENCTINGTHFRKGEAVYPDHNSSEVWNGTFPDGTPFPRNSNKKPRFVFVWKTWGRYWQVAGGPSSSLTIGTGNVPLGSYNMEVVIFHCRGKDKFIPLGYASSQFSITDQIPFAVSLSQMNDLNQSDSNFIQNRAIAFTVTLHDPSQYLQAADISFNWDFGDNSGALISRELTVTHTYLTPGSFQPQVVLQATIPNVGCGTTAAPTSSPQNSNPSSCPLLFVSPAPADPSASPAAVTVNSPTAVNIDLAVPVSPGDDIALAASAQPAETPVEGEAAASAAPAATPVVSAAAPAAQDNTVLLEGTVALAASVEAGASVTPAAAGEAVQTASPEMAVAPAASTTVLAEGGTQAVTPTGAEDVVVVVAKRQAPETPANGCFIYRYGSFSTSVDVIQGIESVNIVEVANVVLETEEEQNAVDLTVTCQGSLPNEVCMVVSDADCAMPVQTVCSAVQQLPECQLILRQFFNDTGVFCINVTLTNNVSLAATSARVSVTTGSSSSTAGTVAMALGILTVACAVAAVAVTYSRRTKQYQPLREDPAGSSSGSSSGSPRRSSFPMLLWNLLGRQTAGESSPLLQGRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA10</accession>
    <name>W5MA10_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MA10">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005219"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005227.1">
        <property type="protein sequence ID" value="ENSLOCP00000005219.1"/>
        <property type="gene ID" value="ENSLOCG00000004351.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4833">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005742"/>
    <dbReference type="HOGENOM" id="CLU_002623_1_0_1"/>
    <dbReference type="InParanoid" id="W5MA10"/>
    <dbReference type="OMA" id="PMAEMNF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004351">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21094">
        <property type="entry name" value="Nup188_SH3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="11">
        <location>
            <begin position="29"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="534"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1515"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1706"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1706"/>
            <end position="1719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1751" mass="196734" checksum="695770411CEDAC35" modified="2014-04-16" version="1">MADSEMCVRTSRELWTILLGRSALREPAQIEAELDRNWERLLSGLCYYKPPSSSAAEKLKSDKEVAQPLKDFGLRISKLLGLDEQQSVQVLQCYLQEDYRGTRDALKSVLQDERQSQALLLKIADYYYEERICLLRCVLLLLTYFQDERHPYRSEYANCVNKLEKDLVSNYRQQFEDLFKAEAPTWETHGNLMTERQVSRWFLQCLREQVLLLEIIFLYYAYFEMAPNDLLAFTKIFKEQGFGRRQTNRHLVDKNMDALVDRIGYFSSLILVEGMDIDFLHKCAIEDRTEQHQFSNTPELNKEMDQIFLTFGDISHHAPVLLAWVLLRHTLSPEDSSPVIRRIGNTSLQLGVFQYLTHMLRALGSSGNNCTASTARMCIYGLISFVITSFEEETLGSQQVVIYFQHLIDAACEVLAAPSLAELFWETKPNMGLGMILDSAVGMFPQKISPLLQILTALLSDKSTAKKVYSFLDKMSFYTEVYKHKPNDILSREDETLWRRQTPKLLYPLGLGQTNLRMPQGTLGQVIPGEEGYMVRWDYSYSSWTLFTCEIEMLLHVVSTADVIQHCKRVKPILDLVHKVISTDWSISDCLLPITSRIYMLLQRLTSVINPPIDVIASCVNCLTVLATRMPSKVWSALHHTGFLPFASTPLSTMAQSISSAEGMKAGNYGNLLILIEQPHGEYAVTIAFLRLVTTLVKGQLGSTQNKGLIPCVLLVLKEMLPTYHKWRYNTYGVRERIGCLILELIHAILNLSPEGESQGRSVPNLQSLCIYSLANTEAGQAVVSIMGIGVDTIDVVMAAQPSSGSSEGPGQMLIQTVKLAFSVTNNVIRLKPPSDVVSPLEQALTQHDAGGHGNNLIAILAKYIYHKHDPALPRLSIQLLKRLATVAPMSVYACLGSDAAAIRDTFLTRLQSKTEDMRIKVMILEFLTVAVETQPGLIELFLNLEVKNGAEGSKEFLLGEWSCLQVVLDLIDSEQQGKYWCPPLLHRAALAFLHALWQDRRDSAMSVLRAKEKFWENLTTPLFGTLPNPSDTTEPCILETCALVMKIICLEIYYVVRGSLEQPLKDMLKKLSSNKRYEYWSNYVKSLVCHMAEMEEDGIRSFTECQMLVSAWRMVLILSTSHSDVMQLTEDSTRLKLFMDVLDGTKAVLLVPTSVPCLRLGSMLATLLLILLKQWKSVIESAPPILPPLALILESILQADRQLMERTKAKVFSALISVLQIQELNGSQVPQLPQLLQCVCETMQEEALALMDSTRHMALTGDSTADDKDSMETDAVPRALHKDQKDGVCVLALHLAKELCKVDEGGDHWLEVMRKIPVLPSILSALELSLRVKQNLYFTEAALHLLLILARTQQGASAVAGAGVIQSICLPLLSVYEITNGGSQGSGFSRKSQDVPSWPGVYRLSMSLMESLLKTLRYNFLNEALDFVGVHQERILQCLNAVRTVQSLACLDEADHTVGFLLQLSNFCKEWQFHLPQLLRDVQVNLCYLCQTCTYLLHSKKMLHHYLQKAKNGDAMPPGHLPRPQRAPQTPSKQLSSEREEAEQKALLAVQYTLLRILSKTLAALRYFTPDFCQILLDQSLDLAEYNILFVLSFTTPAFDSDVAPSFGTLLATINVALSMLGEVEKRKEPASLSVGSLASSEETQALRSLLIFTMENCFYVLISQAVRYLKDPAVHLRDKQRMKQELSSELSTLLSSLSRYFRRGSPSSPAGGLLPSPQSKPTTPGAKPGLEVQEPLIQLVQAFVRHVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA62</accession>
    <name>W5MA62_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zmp:0000001114</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003157">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA62"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005271"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005279.1">
        <property type="protein sequence ID" value="ENSLOCP00000005271.1"/>
        <property type="gene ID" value="ENSLOCG00000004407.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165120"/>
    <dbReference type="HOGENOM" id="CLU_006842_19_3_1"/>
    <dbReference type="InParanoid" id="W5MA62"/>
    <dbReference type="OMA" id="KIIVIQI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004407">
        <property type="expression patterns" value="Expressed in ovary"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="CUB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="8">
        <location>
            <begin position="122"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="396"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="646" mass="70006" checksum="70134E9373B8173A" modified="2014-04-16" version="1">MSFDLYAKYGNNRTLTLTSPKKPYYQWRLRVPSGHVVRLVIVTLNGASPGNCAAHKLSAYDFLLPLQNKIIARWCGVPVTITPPVIKLTSSGNVMLVTFSFNRQKDSAIFKAYFQAVPKAGCGGSLPVWNGTVTSPYYPSHYPPNVDCTWTINAPPRGYLLSVTVVVLDIQDSPASHHCDKDWLDINGVRLCTPVADSSRKRVYSSPVTLHFHSDESITRRGFFILYRAFSRDSPCPKQFQCSNGRCVPLKKLCDGVKDCTDGSDEAKCAACGPGEVNCGNGQCRPHVSLCGGQSSCGDSSEEVDCAGDCFLHCPNGICIPQSSVCDGIVDCKDRSDEINCTRALHKACSPSSYKCLNGRCLSKLNPECDGVKDCADGSDEAGCGCGSRPRKKSKIVGGGDAQTGEWPWQVSLQMGRYGHVCGASVISSRWLLSAAHCFQDSESIRWPGRVPRFLFSSSRQARTALASRSAGRGGAGALSRTAVKRILTHGRYDASSLDYDLALLELATPLEYSGTVRAVCLPPAAHPFSVGQSCFVTGWGLLEEDVGSLSLMLQKAEVQIINQTVCNKYLGNSLTARMLCAGYLTGRVDACQGDSGGPLVCQALTGQWFLAGVVSWGEGCGRWHRPGVYARLTEFRDWIQEQMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBK9</accession>
    <name>W5MBK9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain containing 12a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003131">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBK9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005768"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005776.1">
        <property type="protein sequence ID" value="ENSLOCP00000005768.1"/>
        <property type="gene ID" value="ENSLOCG00000004788.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU3U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063516"/>
    <dbReference type="InParanoid" id="W5MBK9"/>
    <dbReference type="OMA" id="YCSKAQQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004788">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052958">
        <property type="entry name" value="IFN-induced_PKR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289:SF13">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="758" mass="87426" checksum="9784A5B4BA59E322" modified="2014-04-16" version="1">MPNFCAAPNCTRKSTQSDLAFFRFPRDPTRCKLWVENCRRADLEGKTSDQLNKHYRLCAKHFEASMICKSSPYRTVLKDSAVPTIFDLTSHLNNPHSRHRKRIKELTEEELRKIKERRLEASCEQVETNKENEISERQEGGESVEDAPPMTPEEKEIRDYLKSLFEIIVVIGKQNIPLNGHAEHEEGSESFTPSNFQALLEYRINAGDEVLRKRFEMAAVNVEYCPALQQRRMLEVCESCIREELLQEIRECRFFSLVTDHAVEIGGEDHLPLFLRFVDQTNCLREEFIGFLPCEGDDEALADRLLTEITEKWGLNMEYCRGQAHVSSGVFVCKMKSVATRLMEKYPMAVYTPCSTCALNIFLANSLPFTSVQVVMSTLKKIDSFFSKSALARAELEHAISIFHQNNEAKANELKEVCRTSWTERHDTFELAVDLLESLLLCMDSIHFNEDLKWNDRITSEAYVISETLADFEFVVTLVVLKYALSFTRAFGKNLQGQTLDVYFAANSLTAVLHSLNEVMDNIEVYHEFWFEEAINLAAAMEIPVKVPRLYLRKQRAETSLEIQPESYFKEFLSVPVVGRVIQELKDVFSENHLKALKCLSLVPFVMGHLKFNTSEENNADIYKNDLPNPDTLPAELHCWRIKWKHRGKEFSLPSTIYDTLQLSEVKFFPNVYSFLKVLCTLPVLNLENSKYGVARKRLKAYLADTPVSHRSRSLALLNINYDVKHDLDFMVDTYTKMYPEKEEDLYKIKSAETVNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBY8</accession>
    <name>W5MBY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xyloside xylosyltransferase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216702.1">
        <property type="nucleotide sequence ID" value="XM_015361216.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216703.1">
        <property type="nucleotide sequence ID" value="XM_015361217.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBY8"/>
    <dbReference type="FunCoup" id="W5MBY8">
        <property type="interactions" value="600"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005905.1">
        <property type="protein sequence ID" value="ENSLOCP00000005897.1"/>
        <property type="gene ID" value="ENSLOCG00000004904.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697515"/>
    <dbReference type="CTD" id="152002"/>
    <dbReference type="eggNOG" id="KOG3765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158154"/>
    <dbReference type="HOGENOM" id="CLU_048175_0_0_1"/>
    <dbReference type="InParanoid" id="W5MBY8"/>
    <dbReference type="OMA" id="NVWCIFT"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004904">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140560">
        <property type="term" value="F:xylosyl alpha-1,3-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042465">
        <property type="entry name" value="XXLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46612">
        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46612:SF1">
        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="374" mass="42615" checksum="F2E7A10B5E440641" modified="2014-04-16" version="1">MGILRSSPNTMARASAFRSYQLLLLLAAALAVVAFYYFGAERQSFSSTTRRLKETQASLNANRNDADLSSDTGAGHPDLYYHALMMFTKVDRNRGLQEKFRVALRSMAAHGGFADGEVLVLHFVSDAPSRELGERMLPELLRDATFRYEVVFHDVSTLTEKLFPIVEAMQKHFSAGSGAYYSDSIFFLSVAMHRIMPADLKQIVQLDLDLKYRSNIRELFQEFTRFPAGAVIGIAREMQPVYRHTFWQYRRENPKTAVGGPPPDGQPGFNSGVMLLDLEAMRRSALYNQLLEPARVARLADKYHFQGHLGDQDFFTMIGMEHPGLFHALDCGWNRQLCTWWREHGYGDVFQLYYQCDLPVRIYHGNCNTPIPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCA7</accession>
    <name>W5MCA7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DBIRD complex subunit ZNF326</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 326</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the AKAP95 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCA7"/>
    <dbReference type="FunCoup" id="W5MCA7">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006016"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006024.1">
        <property type="protein sequence ID" value="ENSLOCP00000006016.1"/>
        <property type="gene ID" value="ENSLOCG00000004998.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUAZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063777"/>
    <dbReference type="HOGENOM" id="CLU_024193_2_0_1"/>
    <dbReference type="InParanoid" id="W5MCA7"/>
    <dbReference type="OMA" id="XMAFTCS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004998">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044609">
        <property type="term" value="C:DBIRD complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007071">
        <property type="entry name" value="AKAP95"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034736">
        <property type="entry name" value="ZF_C2H2_AKAP95"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12190">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN AKAP 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12190:SF1">
        <property type="entry name" value="DBIRD COMPLEX SUBUNIT ZNF326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04988">
        <property type="entry name" value="AKAP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51799">
        <property type="entry name" value="ZF_C2H2_AKAP95"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2 AKAP95-type" evidence="14">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2 AKAP95-type" evidence="14">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="204"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="416"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="452" mass="50965" checksum="8615DD291C1D3556" modified="2014-04-16" version="1">MSHGCSQRRYAGPVSRPFDSFEGNSLERFGPYESYDSGSSRGSRDLYRSGFGSSFGGGFDSSFPNNQFDNDVSSWESSYLRSGLRSGFMDNAGRGGYSSYGSLSSPHMKPAPVGSRGRGMPAYPQNKFGGRSSDLGGPTAVRGRGRGPMGQFGNPPPGFQIDYQHQPAFRGVKRKMMAPSRPNTFVKKQKLTKTSLAPKGKIFLSVVPGTNSPSEATMEEEKQRKIEARREKQRRRREKNNEKYGDGYRLAFTCTFCKFRTFEEKDIENHFESPSHKETLDYIQKQAKFDDGVINFLHESMVNKFRKTVSRKLNNNHPEQTLEEIHKELMEGVTEEDYMRKVEVIHCVACRAHVPAVLSSVQQHLKSSDHQKNKVLYKEQLKKESVLTATSILNNPIVKARYEKYIKGEDPFEVEAKDEPVEGSQQDSYQNDADLTQFDDGLGEDSVELEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCC0</accession>
    <name>W5MCC0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ripply3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ripply family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCC0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006037.1">
        <property type="protein sequence ID" value="ENSLOCP00000006029.1"/>
        <property type="gene ID" value="ENSLOCG00000005009.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S482">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111429"/>
    <dbReference type="HOGENOM" id="CLU_127183_1_0_1"/>
    <dbReference type="InParanoid" id="W5MCC0"/>
    <dbReference type="OMA" id="PATCQIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005009">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028127">
        <property type="entry name" value="Ripply_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16770">
        <property type="entry name" value="PROTEIN RIPPLY-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16770:SF4">
        <property type="entry name" value="PROTEIN RIPPLY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14998">
        <property type="entry name" value="Ripply"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="148" mass="16484" checksum="E4F4D5ADDB453E16" modified="2014-04-16" version="1">MEPPARTLNVHVTRVCPCCRVSPVGQPGHPAQQKCDSAIWRPWVLTSRDVQLRHGKSDSGSLNSLKKPTSKGAFGFQHPVRLYMPRSKTQEYLRHMGEKVLSSFPVQATIHFYNDDTESEEEEEEEEEYEAGATELTGLGPGDGGTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDR1</accession>
    <name>W5MDR1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + 2 S-adenosyl-L-methionine = a 5'-end (5'-bismethylphospho)-ribonucleoside-RNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58640"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15182"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142777"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + S-adenosyl-L-methionine = a 5'-end (5'-methylphospho)-ribonucleoside-RNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58656"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15181"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142776"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01018761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDR1"/>
    <dbReference type="FunCoup" id="W5MDR1">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006520"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006528.1">
        <property type="protein sequence ID" value="ENSLOCP00000006520.1"/>
        <property type="gene ID" value="ENSLOCG00000005406.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153993"/>
    <dbReference type="HOGENOM" id="CLU_082749_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDR1"/>
    <dbReference type="OMA" id="LNHHDQG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005406">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000632">
        <property type="term" value="P:negative regulation of pre-miRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000138">
        <property type="entry name" value="BCDIN3 domain containing RNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
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        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF1">
        <property type="entry name" value="RNA 5'-MONOPHOSPHATE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="12">
        <location>
            <begin position="42"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="29957" checksum="F47F527E65527F77" modified="2014-04-16" version="1">RTNMAAPAGDGVKEECEQVIQDDPGAARFGNFINYYTFNPPENRLSLIPANLLEELGYSSDNSAPILILDVGCNSGDLSVALYRHLLGQDGSPGETQPSRELHLLGCDLDEALIQRAQQSNPFPETVSFTQLDVTQEQACRAQLGGYLGRFGRASFDLGLCLAVTMWVHLNHGDPALLALLSRLPRLCQHLLLEAQPWKCYRAAARRLRRLGRRDFEHFKELGVRGDVAGQAREHLERRCGMELVRAFGSTAWDRSLLLFRRRGAGGDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDW9</accession>
    <name>W5MDW9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Synapse defective Rho GTPase homolog 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDW9"/>
    <dbReference type="FunCoup" id="W5MDW9">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006578"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006586.1">
        <property type="protein sequence ID" value="ENSLOCP00000006578.1"/>
        <property type="gene ID" value="ENSLOCG00000005452.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234635"/>
    <dbReference type="HOGENOM" id="CLU_005764_1_0_1"/>
    <dbReference type="InParanoid" id="W5MDW9"/>
    <dbReference type="OMA" id="YMVCNAT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005452">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000031">
        <property type="entry name" value="rho GTPase-activating protein 100F isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052118">
        <property type="entry name" value="Rho-GAP_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057459">
        <property type="entry name" value="SYDE1/2_C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 100F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN SYDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25336">
        <property type="entry name" value="C2_SYDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="479"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="635"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="898"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="931"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1018" mass="111965" checksum="FF534774EACB47DE" modified="2014-04-16" version="1">VTVNKMQEWMQRGRMLSSEMKQRIAGSSLCGQGPPSSLAGRAIRESFILVIHSSKQLGAMPTVKSVIVDCLTFQTNENHTDMRLTANSITVSKKRNWLQQSSSRSQTLEDSRVPAAAEEDHGHVAPSPSPSSESQMLEGAVTPLEHRTLRSALPHHRPVDPTEENDADDEGEIWYNPIPEDEEPEVLQGSIPEQSLSAQPLSPPAQGPQSSGAGPRDGDCAGGPLSCDTSEDCQGAPASKMNHTNATHSTECVQLHRQMFTCKPADESTAPLPTGAAHSFKTILLAVTGRAGRISVYLYLIDSPSTGFSPPSSPNPSKKGSSINWSFPDKIKSPGTVRKLSLKMKKLPELSRKLSVKGAPSGSAQPEQQPSSPKSSGSSSGAESSQRRRPHPSPGAGAAASRNVISRYHLDSSVSTQQSFSKKKSSRRSKSASKGGYLSDGDSPELVAKSGKHNAEKAGKGKEAPVSCTQRLPGTEIDIDAFRHYSFVDQPKCSHYISGLMSVHFYGAEDLKPPRIDSRDVYCAIQVDSVNKARTALLTCRTTFLDMDHTFNIDLENAQHLKLVVFSWDPAPRRNKVCCHGTVVLPTLFRVTKTHQLAVKLEPRGLIYVKLTLMEQWENSLDSPEGDHDPVVFGVEAWRVVERENAGLMVPLLMEKCITEIEKRGCQVVGLYRLCGSAAVKKELREAFERDSKGVELSESQYPDINVITGVLKDYLRELPCPLITKQLYEAVLESMAKRPLRMGPRGCENDPSDSELTVGLLDCLPDVEKATLKMLLDHLKLVASCQEVNKMTCQNLAVCFGPVLLSQRQEASSHSNRVFIDSEELASALDFKKHIEVLHYLLQLWPACIRRGKCGHWLNFSLIQGSIFQLSTQYLRRRRQRPQALNLSEAEIAGVLRPRPGRLDSPSNRYAGDWSSCGESYLKPPKEDAGGEADYDDVPSEDAESSEEEEPEQEVEQSLSPPDLPAKQHTYQAYMKIQEISPGLSHQVNLKDLQDSIDTLIGNLERELNKSKLTVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME13</accession>
    <name>W5ME13_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kinetochore associated 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005130">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006622"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006630.1">
        <property type="protein sequence ID" value="ENSLOCP00000006622.1"/>
        <property type="gene ID" value="ENSLOCG00000005474.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007883"/>
    <dbReference type="HOGENOM" id="CLU_231522_0_0_1"/>
    <dbReference type="InParanoid" id="W5ME13"/>
    <dbReference type="OMA" id="FYELYLC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005474">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903394">
        <property type="term" value="P:protein localization to kinetochore involved in kinetochore assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055403">
        <property type="entry name" value="ARM_KNTC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055404">
        <property type="entry name" value="ARM_KNTC1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055405">
        <property type="entry name" value="ARM_KNTC1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052802">
        <property type="entry name" value="KNTC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055402">
        <property type="entry name" value="KNTC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019527">
        <property type="entry name" value="RZZ-complex_KNTC1/ROD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15688">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15688:SF1">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24520">
        <property type="entry name" value="ARM_KNTC1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24516">
        <property type="entry name" value="ARM_KNTC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24515">
        <property type="entry name" value="ARM_KNTC1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24506">
        <property type="entry name" value="KNTC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10493">
        <property type="entry name" value="Rod_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KNTC1 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="KNTC1 first ARM-repeats" evidence="5">
        <location>
            <begin position="355"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="KNTC1 second ARM-repeats" evidence="4">
        <location>
            <begin position="706"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="KNTC1 third ARM-repeats" evidence="3">
        <location>
            <begin position="1283"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="domain" description="RZZ complex subunit KNTC1/ROD C-terminal" evidence="1">
        <location>
            <begin position="1544"/>
            <end position="2092"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2173" mass="247336" checksum="44A5537E9DB4C2C3" modified="2014-04-16" version="1">SPNLYASHSSNGCVVVADKTVVLLDQSCLSVLLQLQFDSDVDTVGLCQEGQFLVVGERNGNIHLNYVPEKKTVLTKALVPKPRCDNEKTYKNLLIQEDTSLPGIYHMYFLVSDGFFQISNLQLEKIHMAIAKTDFGTLKELQNPIKIAFFSTEERHTHGCQHAEIAELANNIQLVIGGSGDHVLSTWKVDLVQKTLCLNNVMDSSLVTGVRKTQILGNVMFVLDNENFLSMWDIYSLVMVWCWPSQCIQDFLLTTEGDSASVATQEDANLKLIILTAQDNTQNRNLTVYSVPSMSLLYSLEVSDVSFLVQTGISTDTIYLLEGISENQLCSSEGMVSAIVLRCLTEALPENRLSRLLYKHKFEEAEQFAIQFGLDVELVYKVRLNFVLEKLASVSAGGYGQAVWMELVEEAKTNLHKIVDEQFTVQYCISTPWPTFDTAQEMLNYAKNKLLKRDEQKTSAYIFAPQVLEALAKLTTFYGSCGPEKFSGISWIEFLNSTDLLSDIMWYLKERDLRSAQYLWLRHQPEFERKFDENMLETLLSTIPEDVPSRDLCLWFKSVITPFVRRIVPKGQKSLVRWLEQRSRNLELTEKSNWPENGLEMAELYFVSRNPNELGLSSSWLWIPLKEDGDCEEVEQLKALVRNLRQMLDLYKKYNCRLPLSDFEKENMTTIAFLMLDKVLAPELIPSTMETVIKPYAAEHNLQLEELLLQYIKDLLERCSSRTASLFETEGEAKAMAVLSCMTDTDLIFDAVLQVMYKAVVPWSEAVEHLVQQHLEMDHPKVLQLQESYRLMEMKKLLRGYGIRGFSLSDDKQAMQMVKYILKQDLPSSLDDALKVAEAYKLSTIEIYILRIVHFISQGKREECLNLLKRLPGEEAECIAERLAMWARLELQDQTDVSEEKRKTHQIAVAHVMVEILKFLLSIQKDNTLKRMQCENNLKMFEAIAHLQEDFDIFLSLEDYESPAVLAQFQEEHIRAYESTRSRARTEKASVVAKDPDGKTKTISTEAGLYRLALLLQRSEEELGADLAMRALAVGKVEKALKICCELYQHHCNEKTGQVLFNAARDLCQMLKANTPMIIPDGMNLPAVIYELACQAATICSADLLLDCQELCKNTLAAMDVYRQCQIDDYGFISKTSSLGTDRDPYGEWTFEDFFNEDGIVLDPITVLPVLYKITTSFVPHSEDSKLYPLDCSCLSNCSYTEGKNHLRQIKGPINEMVQNLHECSQLELALRLMMNCFGTCLQHITSNNMDLTLSSKLHDLQHLLEDRNFVVSLGERTISVIKDVSLALLHKVFNCRVVDCDLAIGYCCLLPKKDVFEKLWHVINNTWQNYNKILAVAAIGAHLSVLYGEGEEHQKFLSLIADAEWGIELGKLGISFQAVFRQTPEKKRDLISTLVKNRNVNSELILKYCRTFNLETDSTLNLYIKTLLLHGTSIDQSEDEPATDIGSTLDHSDALARALKIIPLLKSANELVISLNTAIIKLSPYDYETIEGILRVIQTTDEKITSFHLDQALGLLQHLKSYKRVSTPMDIEHQYLQENSLPRSPLTHTRLPFHLIFFKTSLYFWKIISAELSEETFPTLLLISKLMKVCLDKLYMSAVNHVFDKRLKPQSLKPSNGRKPHVMTKETIKTMETIKTYLLSIKNPEWAAAAAHKIAQELPTGILKTTSLNFCLLLAEKWLKTSSPEDPAHAKAEIFLEKLKLQYQRSATENTLISYHLNSPEYLKSTGFPAKLIVSLYEHSSIEQRLKNPAGRNYPDIHAVAREIASINNIDLSKIRDLLLDKWLCQSTAPAVKVSTSFNIQEDPELMRVIYLLQMYPMDFSARMLYAIVTAEVSPISNSGPRLTFGNRSRALMCLIQIADTKTLESLLKTSIEKIKHYLKYCIYLSEFEVLNIPYTVESFHSSPKEGMIKGLWKNHSHEPRAVRLVAELSLEYQVYDPQLWNGVLQKLISFSMTCYLRKVLVALTAVHSLWQVPNFIRAWKSVILAPFLSASIPLSPQQLEMFYQTFVLLLKCPVLFDLDLIGIAKQFAQFDLPAFTLGSLLLIPCSEKKEQQVQGFLSSSNLVTVLQQVEEHMNTGEVAGFSTQIKDMVLNFMCRNKLYEKIVSSKLFPLLKHHVIVTNQVKDLMDYLVNSNRLDDAATLAADHMKLRGKHVPANMTALEVLQEFLKNYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME81</accession>
    <name>W5ME81_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Si:dkey-238d18.5</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME81"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006690"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006698.1">
        <property type="protein sequence ID" value="ENSLOCP00000006690.1"/>
        <property type="gene ID" value="ENSLOCG00000005542.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286924"/>
    <dbReference type="HOGENOM" id="CLU_020480_1_0_1"/>
    <dbReference type="InParanoid" id="W5ME81"/>
    <dbReference type="OMA" id="YVFRMER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005542">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484:SF7">
        <property type="entry name" value="MYELIN-ASSOCIATED GLYCOPROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484">
        <property type="entry name" value="SI:CH211-171H4.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="147"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="318"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="511" mass="56739" checksum="7B641BBB9A9DA731" modified="2014-04-16" version="1">SLLFDVNLDDLCCVVKVCRVIHVSSTFNEWTAETPFQLSALLGSCVVIPCNFNYPGSERSVSQMTGIWLTESYEYIYHPSGSVIDRFRGRTALVGDLGQKNCSLKLDPVKQKDSGHYFFRIEIHGLDNYSYAKNKTSVFVKDTADPPSISLKGEATAGIDLSASCSVYHTCPVHPPTITWSHNSMMPTVYSEPMGNGQWNLTSVLTFKPQASDHRKTLTCRSEYRGRKAAENSIILNVTCFKPVVELKGHISFLLLLQKNRIPPYYSEFWINAKLNLFCDLQILWFKKGGGISSLQSCPSHILDDRDSELKFLKPDAPVNVRMEFNQRGIKEGDSIIFRCSSDSNPPAHSYQWYNVKGVQTVRLQSERDTFTVDNVTRHSGAFYCTAQNRLGQSNSTAIQSNVEYAPEIKGSSCNLGNSWIICQCIVDSNPPAEIKWLFPDGTLLNRSSERQDSVTVDTLQGAVGFIQSISCYARNTHGNSTVELYVPSAGSALYVALPVSILVVAVIGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFB5</accession>
    <name>W5MFB5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MTSS I-BAR domain containing 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFB5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007074"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007082.1">
        <property type="protein sequence ID" value="ENSLOCP00000007074.1"/>
        <property type="gene ID" value="ENSLOCG00000005856.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRG4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183156"/>
    <dbReference type="InParanoid" id="W5MFB5"/>
    <dbReference type="OMA" id="WEDFNAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005856">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07643">
        <property type="entry name" value="I-BAR_IMD_MIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22060">
        <property type="entry name" value="WH2_MTSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000010">
        <property type="entry name" value="Metastasis suppressor 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF8">
        <property type="entry name" value="PROTEIN MTSS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="608"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="775"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="253"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="538"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="645"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="791"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="834" mass="89376" checksum="AB5DE712BBBCF06C" modified="2014-04-16" version="1">METVEKECGALGGLFQAIVNDMKSSYPIWEDFSAKATKLHSQLRTTVLAAVAFLDAFQKVADMATNTRGATRDIGSALTRMCMRHRSIEAKLRHFTNALMESLITPLQDRIEDWKKTANQLDKDHAKEYKRARHEIKKKSSDTLKLQKKARKGRGDLQPQLDSALQDVNDMYLLMEETEKQAVRRALVEERGRFCTFISLLQPVVNGEIAMLGEITHLQAIIDDLTVLTADPHKLPPASEQVIKDLKGSDYSWSYQTPPSSPSSSGSRKSSMCSSVNSAHSSASRSSSGSQTHSPSSSYRYRSLAQPPPGGAQRLSSVSSHDSGFISQDAAVYSKPPSPMPSDITSQKSSSSASSEASETCQSVSECSSPTSVSSTSTAGAWPLIHSYLPHCPVDHGHFGSSFATFRPAISHTGSIRPFSVILPASPPYNCPPGSNTYSPTSKVPCWKDWSKPGPYDQPLVSTLQRRKEPIERLRESEQGPPPPGYAGLHPEDPQRTRMTPATIAAKHGEEVSPAASDLAMVLTRGLSMEHQKSSRDSLQYSSGYSTQTTTPSCSEDTIPSQVSDYDCYSVNGDAEGDVQTEFDKSSTIPRNSNIAQNYRRMIQTKRPASTAGLPSGVGAPGAHPGGGGGGGGGSGTPGVATIRRTPSTKPSVRRTLSNAGPIPIRPPIVPVKTPTVPDSPSYTGPPARVGSEECVFYADDTSPNAMDYVKASPKRLSLPNTAWGSGAGLEMSVYAQPPPPLSTEEDLLLAANRHSLVEKIGELVASAHALGEGQFPFPSLPGDDPGSDPQAPPPGPAPSQDPAPGGEDMLVAIRRGVRLRKTLTNDRSAPRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFZ4</accession>
    <name>W5MFZ4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosomal protein S6 kinase related</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFZ4"/>
    <dbReference type="FunCoup" id="W5MFZ4">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007303"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007311.1">
        <property type="protein sequence ID" value="ENSLOCP00000007303.1"/>
        <property type="gene ID" value="ENSLOCG00000006050.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160082"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="InParanoid" id="W5MFZ4"/>
    <dbReference type="OMA" id="FPYEMPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006050">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="96"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="396" mass="44799" checksum="140C7F84071DE28F" modified="2014-04-16" version="1">MGADVSNHSKGQPLLEDPRCAPGWRGLLSSVGLSFPAGLCRWGGLRFAPRRTITEGRRPEPPAPEEPFLEWPLPGFISLFLPEFPHRSRLPGPERFQILGCIAKGSYGPILKVRDRTQQRTYAVKVLPKCEILRHGVLAQSKEEVTIQRQVRHPFLHTLQDCWQTQRHLFIMCEYCSTGDLYTYWTMAGRFGEEAVRVFAAELGSALGFLHDFGIIHRDVKMENVLLNDQGKKRVAVEPQTRQASQSGTLTALCRPSSPTAPEVLSGGPYNHAADWWSLGVLLFSLATGKFPVAPRPDHRSMLQEVRSCGYEMPDTFSPELGLLISELLCQNPMCRLRRLERFRKQPFFRGVTFDPQLLQKTPVCLILELKQRPDRPARAGRGLELDVFKTFDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG49</accession>
    <name>W5MG49_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG49"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007358"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007366.1">
        <property type="protein sequence ID" value="ENSLOCP00000007358.1"/>
        <property type="gene ID" value="ENSLOCG00000006091.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002407"/>
    <dbReference type="HOGENOM" id="CLU_117340_1_0_1"/>
    <dbReference type="InParanoid" id="W5MG49"/>
    <dbReference type="OMA" id="IDCADFP"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006091">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00922">
        <property type="entry name" value="Cyt_c_Oxidase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013288">
        <property type="entry name" value="Cyt_c_oxidase_su4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF13">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01873">
        <property type="entry name" value="CYTCOXIDASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="178" mass="20597" checksum="BE41D9CDCA550DDE" modified="2014-04-16" version="1">MDSDVPAVAAARREPGRGTAAGVRGSVRTASHGHGVAKQADMSLPMYWDRRDTPLPDVPYQRELSGPQESLKLKERGSWKELTREEQIALYRMMFKQSYAEMKKPSNEWKTVVGGVFIFIGITSLVVWWQRVFVYPPRPHTLTDEWKAMQAKRMIDMRVNPVEGLSAKWDYERKEWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGE5</accession>
    <name>W5MGE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGE5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007454"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007462.1">
        <property type="protein sequence ID" value="ENSLOCP00000007454.1"/>
        <property type="gene ID" value="ENSLOCG00000006172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6PI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00830000128446"/>
    <dbReference type="HOGENOM" id="CLU_077975_8_1_1"/>
    <dbReference type="InParanoid" id="W5MGE5"/>
    <dbReference type="OMA" id="YCALRVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006172">
        <property type="expression patterns" value="Expressed in mesonephros"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002173">
        <property type="entry name" value="TRADV8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867751" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="13"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="13369" checksum="67519E99B24C67DD" modified="2014-04-16" version="1">MEKILFILMFIIPAGMTAEDLISSLQTKVSRKEGESVTLRCSYDTSDEYVYLYWYRQYSDQAPQYILRKGARSYSNTHTADFAKDRFSSTADRTFTTLTISDLTVGDTAVYLCALRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGP5</accession>
    <name>W5MGP5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Epstein-Barr virus induced 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EBI3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGP5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007554"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007562.1">
        <property type="protein sequence ID" value="ENSLOCP00000007554.1"/>
        <property type="gene ID" value="ENSLOCG00000006256.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXJ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160050"/>
    <dbReference type="HOGENOM" id="CLU_047259_2_1_1"/>
    <dbReference type="InParanoid" id="W5MGP5"/>
    <dbReference type="OMA" id="CEVNPER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006256">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045523">
        <property type="term" value="F:interleukin-27 receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042098">
        <property type="term" value="P:T cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056621">
        <property type="entry name" value="FN3_IL27B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003530">
        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053073">
        <property type="entry name" value="IL11/IL27_subunit_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483:SF2">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24031">
        <property type="entry name" value="FN3_IL27B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01354">
        <property type="entry name" value="HEMATOPO_REC_L_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868165" evidence="5">
        <location>
            <begin position="21"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="130"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="172" mass="19112" checksum="6A60C5C322DB65E8" modified="2014-04-16" version="1">HAVLLGAALFLGAASQSAHGKAEPPLALYSLPQLDVQCWSPSYPQRVLCTWKVQPEPDLPTSYTATYRKGLLGVQIPCEVNPERPRSCVMENLEMFSVEPYFVNVTATNALGSTTRLLPFILEKIVKPDPPVDIKVTLRSSRRLTVEWAPPPSWTDPTLFQLKYQVQYSDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGQ1</accession>
    <name>W5MGQ1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylcholine:ceramide cholinephosphotransferase 1</fullName>
            <ecNumber evidence="12">2.7.8.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Sphingomyelin synthase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphing-4-enine 1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:36079"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:73203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.27" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01004122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGQ1"/>
    <dbReference type="FunCoup" id="W5MGQ1">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007560"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007568.1">
        <property type="protein sequence ID" value="ENSLOCP00000007560.1"/>
        <property type="gene ID" value="ENSLOCG00000006259.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158306"/>
    <dbReference type="HOGENOM" id="CLU_027104_1_0_1"/>
    <dbReference type="InParanoid" id="W5MGQ1"/>
    <dbReference type="OMA" id="HWPLRCP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006259">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006686">
        <property type="term" value="P:sphingomyelin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09514">
        <property type="entry name" value="SAM_SGMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000040">
        <property type="entry name" value="Phosphatidylcholine:ceramide cholinephosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF28">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="17">
        <location>
            <begin position="18"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="417" mass="49014" checksum="3EDAEAA8C8875716" modified="2014-04-16" version="1">FPLPHPCLETEMKKLVSWSAAEVSHWLTEEGMQEYSEHFRHLNGQALLQLTEEDFRKPPFSLVTSDNGRQLLDRIETLKIEHHIEVHKNGHANGHLIMGGDGDATSKAKRNGVPNGFRKEMVQIPMPEPEHSPYPLEWVKTGVAFFYALSCFVLTTVMISVVHERVPPKEENPPLPDKFFDLFDRVEWAFSICEINGMVLVGLWLLQWILLKYKSIIGRRFFFIVGTLYLYRCITMYITTLPVPGMHFKCSPKLFGDWEAQMRRVMKMIAGGGLSITGSHTMCGDYLYSGHTVMLTLTYLFIKEYSPKRFWWYHWICWTLSAVGIFCILLAHDHYTVDVVVAYFITTRLFWWYHTMANQQVLKETSQSNFFSRVWWYRLFQYFEQNVHGIVPRNYQWPFSWRTLKYNGAVKYSRIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH32</accession>
    <name>W5MH32_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000007691"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007700.1">
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        <property type="gene ID" value="ENSLOCG00000006373.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015248"/>
    <dbReference type="HOGENOM" id="CLU_1340265_0_0_1"/>
    <dbReference type="InParanoid" id="W5MH32"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006373">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866132" description="Small ribosomal subunit protein mS29" evidence="8">
        <location>
            <begin position="17"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="205" mass="23130" checksum="9E350F299B1CB67C" modified="2014-04-16" version="1">HHTPSLLWYLLHHTHTLSLCAHPHYSCIALSPSLQWYLSHHTHAHSLTTHHTHCSCAHLTTSHCRHPHSPSLPVSLPAHLWVKNCRELLPSSSRPSRFDQPIQASQWLRNFRVTNERFLSQIKTTRQYVWSKRESTDAGRPLGELVDQGLTRVKSSSDVASALMRELRGQCGGGAFRLAVAVDGVNALWGRTALRKEDKSPVRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHI4</accession>
    <name>W5MHI4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cysteine protease</fullName>
            <ecNumber evidence="18">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01015231">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHI4"/>
    <dbReference type="FunCoup" id="W5MHI4">
        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007843"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007853.1">
        <property type="protein sequence ID" value="ENSLOCP00000007843.1"/>
        <property type="gene ID" value="ENSLOCG00000006470.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063000"/>
    <dbReference type="InParanoid" id="W5MHI4"/>
    <dbReference type="OMA" id="PDETFHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006470">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF39">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 18" id="KW-0645">Protease</keyword>
    <keyword evidence="12 18" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="19">
        <location>
            <begin position="44"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="406" mass="45797" checksum="187CEB9F511A1F87" modified="2025-10-08" version="2">MSVHCVAATLTYDTLRFGEFEDFPETEEPVWILGREFSALTEKDEILSDIASRLWFTYRRNFQPIGGTGPTSDTGWGCMLRCGQMILGQALVCRHLGRDWRWIRGQKQKEEYFSILNAFIDKKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLSAFDSWSQLAMHVAMDNTVVIEDIKRLCMPWLDFAGAAAAPGLEGERELNGCAEGACALAGAEGEAALWRPLVLLIPLRLGLSDINEAYIETLKNCFMLPQSLGVIGGKPNSAHYFIGYVGDEMIYLDPHTTQPAVEPSDDGQVPDDTYHCQHPPCRMHIRELDPSIAVGFFCHTEDDFDDWCMRIRKLSGSREGLPMFELVDSQPSHFTSVLPRTALKYTWSCCKNSDRLERFFDSEDEEFEILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHY7</accession>
    <name>W5MHY7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01006007">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006008">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHY7"/>
    <dbReference type="FunCoup" id="W5MHY7">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007996"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008006.1">
        <property type="protein sequence ID" value="ENSLOCP00000007996.1"/>
        <property type="gene ID" value="ENSLOCG00000006621.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157430"/>
    <dbReference type="HOGENOM" id="CLU_006650_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHY7"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006621">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14569">
        <property type="entry name" value="DSP_slingshot_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11652">
        <property type="entry name" value="SSH-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE SLINGSHOT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23040">
        <property type="entry name" value="PH_SSH1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="12">
        <location>
            <begin position="251"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="310"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="375"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="461"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="518"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="630"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="693"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="834"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="981"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="636"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="737"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1066"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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            <dbReference type="ARBA" id="ARBA00048336"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
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            <dbReference type="PROSITE" id="PS50056"/>
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            <dbReference type="PROSITE" id="PS51998"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1092" mass="119039" checksum="47B6A6D59FC4EF54" modified="2014-04-16" version="1">MALVTVQRSPTPSNTSSPCVSEADSGEEERRSQPRSFSESFLTVKGAALFLPRGNGSPASSTPRASQRRNKHAGDLQQHLQTMFTLLRPEDNIRLAVRLESAYPQCTRYMVVVSTNGRQDTEESIVLGMDFSSRDSSTCTVGLVLPLWSDTLIHLDGDGGFSVSTVNRVHVFKPVSVQAMWSALQSLHKACEVARCHNYYPGSLFLTWVSYYQSRISSDQMCVNEWNAMRDVQSHRADSPVLFTDVPTERERTERLIKTRLREIMMQKDLENVTSKEIRTELEMQMVCNLREFKEFIDNEMIVILGQMDSPTEIFDHVFLGSEWNASNLEELQNSGVRYILNVTREIDNFFPGMFEYHNIRVYDEEATDLLAYWNDTYKFISKAKKAGAKCLVHCKMGVSRSASTVIAYAMKEYGWDLERAFDYVKERRAVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSEHHEPLCKPQLSLGPRDTRRSSSSSNNNNSTPQSLEHFLGMAVLQALTGSPAETEAEAGRDELSPSGEAPATAYQDQPTSNGLCDLSPAEDLPSPLLDPPLLPLLLSIPMPHLMMARSEVAPALPEHKAESPVLSASVPVPRPATVEGMGVGDVGPGYPMSPGTPMAVSPLSPPGAAVPGAPSAEDDINNNDTGGDTGSAGSSSLSTDSIDFFSAREKFLELSQDGRARTLSNSRPEEQAAGRTPYPKGPGQELPLSEDPSATPSAEEPPKEYDSPHCARHPPGLTGLSSSESERVRHLKYESAFSLETGAALLLWCPLVARVFSFLSPPPCQEPPGTEPGCAVPHSQSENGVSVRHIVTELESISQASGGKGEPAAAAAAAPPPGDPDPGQGLPPGEKGAPRTEPPPAPPEPVAFLRLEGATEHSSDTDPPAALPPPCRAEHPERIRETLQAFLCEAAEGQELSTVRQRALEIEARIRRAGLTPPSLMKRSASLAKLGCLELSADDLCQWDSQTPVPTSPAGARQPEALPCPSPEDASKKQRVVLRTPEHETRPASPQSTLDFQQGGEEGLPLSTGHAPGNTPPPAPLVAMAARQQHGRTHPLRRLKNSDKRRSSSSLYNTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI65</accession>
    <name>W5MI65_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">DS cell adhesion molecule like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01038555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008074"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008084.1">
        <property type="protein sequence ID" value="ENSLOCP00000008074.1"/>
        <property type="gene ID" value="ENSLOCG00000006671.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155354"/>
    <dbReference type="HOGENOM" id="CLU_001038_0_1_1"/>
    <dbReference type="InParanoid" id="W5MI65"/>
    <dbReference type="OMA" id="GHTMELP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006671">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd05734">
        <property type="entry name" value="Ig_DSCAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20958">
        <property type="entry name" value="IgI_5_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule"/>
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    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule a"/>
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    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000104">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000167">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
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    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000219">
        <property type="entry name" value="Down syndrome cell adhesion molecule homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000229">
        <property type="entry name" value="Down syndrome cell adhesion molecule homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000264">
        <property type="entry name" value="Down syndrome cell adhesion molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DS cell adhesion molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF72">
        <property type="entry name" value="CELL ADHESION MOLECULE DSCAML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1628"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="155"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="266"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="354"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="448"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="546"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="636"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="730"/>
            <end position="824"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="828"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="923"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1025"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1129"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1229"/>
            <end position="1324"/>
        </location>
    </feature>
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        <location>
            <begin position="1314"/>
            <end position="1403"/>
        </location>
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        <location>
            <begin position="1419"/>
            <end position="1513"/>
        </location>
    </feature>
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        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1752"/>
            <end position="1778"/>
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    </feature>
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        <location>
            <begin position="1817"/>
            <end position="1841"/>
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            <begin position="1877"/>
            <end position="1900"/>
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            <begin position="1984"/>
            <end position="2026"/>
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            <begin position="495"/>
            <end position="510"/>
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            <begin position="1769"/>
            <end position="1778"/>
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            <begin position="1817"/>
            <end position="1826"/>
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            <begin position="1877"/>
            <end position="1899"/>
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        <location>
            <begin position="2010"/>
            <end position="2021"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2084" mass="228510" checksum="6CCFBE85FB32FF33" modified="2014-04-16" version="1">GLERSPGIIVTTPARSCAGPITARSCRLCTTFLRNTPAIYGMWLVTFFLLYSLREVRSEDAGTRLYFVNATLQQVTFSNSVGVSLPCPAGGSPAAALRWYLATGDDIYDVPHIRHVHANGTLQLYPFLPSAFNSFIHDNDYFCTAENSAGKIRSPSIHVKAVFREPYTVRVDDQRATRGNVAVFKCLILSAVQEYISVVSWEKDTVSIVPGNRFFITSYGALYISDVQKEDALSTYRCITKHKYSGETRQSNGARLSVLDPMENPPSILDRFQSGEVWAGLSVELPCIASGYPNPAIRWLKDGRPLPADSRWTRRLSGLTVSDLRPEDSGNYICEVTNSFGSAEVRGHLNVIDPLRVTLTPKNLKTGISNTVILTCAVQGSPHYTVQWYRNTELVVPDDHISIRGINNETLLINSAQKTHSGAYQCFATRKGQTAQDFSIILLEDGTPRIVSSFSERVVAPGEQFSLMCAAKGAPPPTVTWTLDDEPITRDSGHRSNQYTLSDGSTVSHMNVSSPQIRDGGVYRCVARNSAGSAEYQARINVRGPPSIRAMRNITAVAGRDTFINCRVIGYPYYSIKWYKDSLLLPDNHRQMVYDNGTLKLMDVQKGMDEGEYLCSVLIQPQLSISQSVHVTVKVPPLIQPFEFPPASIGQLLYIPCVVSSGDMPIRITWRKDGQVIMSGSGVTIETKEFMSSLQISSVSLKHNGNYTCIASNDAATVSRERQLIVRVPPRFVVQPNNQDGIYGKAGVLNCSVDGYPPPEVMWKHAKGSGNPQQYHPVPLTGRIQILANGSLLIRHVLEEDIGYYLCQASNGVGTDISKSMFLTVKIPAMITSHPNTTIAIKGQQKELECMARGERPIIIRWEKGDTVIDTDRNTRYSITTKDNAEEVVSTLKVSCVCRGVSVWIVQYSVSLCQCLYCTVQQPPDPPELEVKEVKARSMNLRWTQRFDGNSIITSFDIEYKNKSDSWDFKHATRNISPTINQANIVELHPARVYSIRMYSFNKIGRSEASKELTISTEEAAPDGPPLDVTLQPMTSQSIRVTWKAPKKELQNGVIRGYQIGYRENGPGSNGQYTISNMKATGDSEVYTLDNLKKFAQYGVVVQALNRAGTGPSSTEINCTTLEDVPSQPPENVRVMSITSDVAVLSWSEPPRITLNGVLKGYRIIYWSIFPDGEWGEMQNITTTRERVELKGLEKFTNYSVQVLAYTQAGDGVRSRMLNVQTKEDNPGPPAGIKAVPSSASSVVVSWLPPTKPNGIILKYTIFCSSPGSGQPAPSEYESSPEQLFYRIAHLTRGQQYLIWAAAVTSAGRGNISEKVTVEPAGKAPAKIISFGGTVTTPWMRDVRLSCTSVGEPAPTIKWTKDSEDLAIPVTVDGHRLILANGTLVLRAVKAEDSGYYTCTATNTLGFDTIIVNLLVQVPPDQPRLTVSTTSTSSITLAWIPGDNGGSSIRGFVLQYSVDNTEEWKDVFISSTERSFKLDNLRCGTWYKVKLAAKNSVGAGRISEIIEAKTHGREPQFNKDQHLFTHINSTHARLNLQGWSSGGCPITAILLEFRPKGTWAWHALRTNITSDVFLAELREATWYELKMKACNSAGCGNETSQFATLDYDGSTIPPIKSARGEGDDVKKLFSIACPVILVTLGVALLFIIRKKRKEKRLKRLRDAKSLAEMLISSKNNRSFDTPVKGPPQGPRLHIDIPRVQLLIEDKEGIKQLGEDKATIPVTDTEFSQSVNPQSFCTGVSLHHPALIQNTGPLIDMSDIRPGTNPVSRKSVKSAHSTRNRYSSQWTLTKCQASTPARTLTSDWRTVGSQHGITVTESDSYSASLSQDTDKGRNSMVSTESASSTYEELARAYEHAKLEEQLQHAKFEITECFISDSSSDQMTTGTNENADSMTSMSTPSEPGICRFTASPPKPQDYDRGKNVAVPIPHRANKSDYCNLPLYMKTDPFFRKQPELHDPCPVVPPREASIRSLAARAYHTQAARHMTLDPGSSSKQALTLAHPGVGMGGSSGTATLPQRTLTMPGSAAPSTSAAAAAASASASGANPTAAAKIGGSRDSLLEGNSAGLGRLQKQGAGAYSKSYTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK76</accession>
    <name>W5MK76_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myosin heavy chain 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK76"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008785"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008796.1">
        <property type="protein sequence ID" value="ENSLOCP00000008785.1"/>
        <property type="gene ID" value="ENSLOCG00000007263.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155421"/>
    <dbReference type="HOGENOM" id="CLU_910713_0_0_1"/>
    <dbReference type="InParanoid" id="W5MK76"/>
    <dbReference type="OMA" id="CEIKMAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007263">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="12">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="178"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="271"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="306" mass="34053" checksum="87C0FCAEEA5B5482" modified="2014-04-16" version="1">MIALSSSQFCMRRCQGRLFRPAPCCPDPVACGGRGAGQAALPLPPAGRLRLNTPKEGEDGNLDSSSVEYSPQAQDSPNPDRQQPRFSPCEIKMAKKPGTALSEDEKFLFLDKNINSPLAQADWSAKKLVWVPSERHGFEAASMKEEHGDEVLVELTENGKKVTFNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKMLPIYSEKIIEMYKGKKRHEVPPHIYSITDNAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDTSAVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML30</accession>
    <name>W5ML30_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pannexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PANX</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Structural component of the gap junctions and the hemichannels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the pannexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009089"/>
    <dbReference type="GlyCosmos" id="W5ML30">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000009100.1">
        <property type="protein sequence ID" value="ENSLOCP00000009089.1"/>
        <property type="gene ID" value="ENSLOCG00000007487.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT58">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153972"/>
    <dbReference type="HOGENOM" id="CLU_050054_1_0_1"/>
    <dbReference type="InParanoid" id="W5ML30"/>
    <dbReference type="OMA" id="TESAHMG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007487">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032732">
        <property type="term" value="P:positive regulation of interleukin-1 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000990">
        <property type="entry name" value="Innexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039099">
        <property type="entry name" value="Pannexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15759">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15759:SF5">
        <property type="entry name" value="PANNEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00876">
        <property type="entry name" value="Innexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51013">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="13">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="12">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600990-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600990-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU010713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="433" mass="48887" checksum="7508738551FB126B" modified="2014-04-16" version="1">MAIAHLASEYVFSDFLLKEPSESKYKGVRLELAVDKMVTCIAVGLPLLLISLAFAQEVSVGTQISCFSPTNFSWRQAAYVDSFCWAAVQQQHLSHSDAGLLPLWLHKFFPYVLLLVAILMYVPSLFWRFTATPHLFSDLNFIMEELDRSYNRAIKLAKNLVTSGVVDSSLPGDACRMKKNSLDRNEGCLKYPLVEQYLKTKAASKGLIIKYLICRILTFVNVLFACIYLGYYINLASITDEFKCNIRTGVLKNDTVIPKFLQCKLIAVGIFQLLSYINLIVYILLAPAIIYTTLAPLWKKADFLKVYQILPTFETFSITSGFYNDLSLYLLFLDENLSELKSYKRLKVLEHLQNNGEEPFDLMCLLTNLGQVKTDAVDGKILQNQSNNRVAGAEPENDHVQLQGKNVTSKNLSLADGNNEDTSVRQRMLDSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLE7</accession>
    <name>W5MLE7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COG4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLE7"/>
    <dbReference type="FunCoup" id="W5MLE7">
        <property type="interactions" value="1657"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009206"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009217.1">
        <property type="protein sequence ID" value="ENSLOCP00000009206.1"/>
        <property type="gene ID" value="ENSLOCG00000007581.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154065"/>
    <dbReference type="HOGENOM" id="CLU_014853_2_0_1"/>
    <dbReference type="InParanoid" id="W5MLE7"/>
    <dbReference type="OMA" id="RASECQQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007581">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006029">
        <property type="term" value="P:proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000002">
        <property type="entry name" value="Conserved oligomeric Golgi complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1970:FF:000001">
        <property type="entry name" value="Conserved oligomeric Golgi complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="13">
        <location>
            <begin position="147"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="26"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="745" mass="83992" checksum="F0DD3ABB36C6EB59" modified="2014-04-16" version="1">MAESSSAPAPRPSGGLARSSAVTMDAIEAITELEELERTYQRLCDEERETESQLEALVSQQSNIESKMQALQRMGPNLQLIEGDASQLSGMISFTCSLAENVSSKVRQLGISQSRLLSFPTSPFFFLYPLSLSLCAGSAVDASLSLLKEAEQRLKTLVRERLDQAIKAGDLAQVERFFKIFPLLGLHEQGLARFAQYLCSQLASKAEENLLLAVGGELSERRAALVFADTLTLLLEGIARVIETHQPIVETYYGPGRLYMLIKHLQGECDRQAEKVVDKFIQQRDYHRKFQIVQNSMMRTAATERIEPRDLDPVLLEVTMMNARAELYLRFLRRRVTADFEVGDGTAPESTVQEHQQSLEKLLKHCLLSRNMQELIGYYIPMEEYYMRETVNKAVAMDTYEKGQLTSSMVDDCFYIVKKCIGRALSSSSIDCLCAMINHSTSVLESDFREVLYNKLRQGFPATTLQDIQRGVSSAVSLMQSSLQQGKFNTMGIESSEEAKTSFLVTLNNVEVCSENIMTLKLNLENDCAKLFNQGFGGGEQARAKIESCLSDLVSVSSKFKDLLQEGLTELNNTAIKPQVKPWVSSFLSISHNIEEEDFNEYEANDPWVQQFVVNLEQLMAEFKAGLSPVIYDILTSLMTSLIALELEKIVFKCTFSRLGGLQFDKELRSLVAYLTTVTTWTIRDKFARLTQMATILNLERVTEILDYWGPNSGPLTWRLTPAEVRQVLALRIDFRSEDIKRLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLM0</accession>
    <name>W5MLM0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-type tyrosine-protein phosphatase C</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Leukocyte common antigen</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01016047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009279"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009290.1">
        <property type="protein sequence ID" value="ENSLOCP00000009279.1"/>
        <property type="gene ID" value="ENSLOCG00000007650.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683459"/>
    <dbReference type="KEGG" id="loc:102683459"/>
    <dbReference type="CTD" id="5788"/>
    <dbReference type="eggNOG" id="KOG4228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159457"/>
    <dbReference type="HOGENOM" id="CLU_001645_2_2_1"/>
    <dbReference type="InParanoid" id="W5MLM0"/>
    <dbReference type="OMA" id="ECEDYNC"/>
    <dbReference type="OrthoDB" id="5794147at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007650">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14558">
        <property type="entry name" value="R-PTP-C-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14557">
        <property type="entry name" value="R-PTPc-C-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000033">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000042">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF539">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866235" description="Receptor-type tyrosine-protein phosphatase C" evidence="19">
        <location>
            <begin position="26"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="20">
        <location>
            <begin position="651"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="21">
        <location>
            <begin position="830"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="20">
        <location>
            <begin position="942"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="21">
        <location>
            <begin position="1133"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="28"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="988"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1234"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="116"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="996"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1260"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1291"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1309"/>
            <end position="1321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1321" mass="147699" checksum="3B95CE7780A1BE4C" modified="2014-04-16" version="1">MTGLFGLKLTLCVGLLVLGIVCEDAGPTSPTVAHSNTTETSTTVNPTISTSNQTHNATSLKPAGATSHFNVSPTSTFPGSSDLPSTVPSSLPAHTVTSTAQSNVSDSTTPHNSTLTGRTDSTSSISSASTRSGSSDLTSTVSSSSPPSTVTSTANSSVSNSTTPPTPTSSGGTSSNISTTVSSSSPTVTVNTTAQTLVSNSATSPTPSAADNLEILVCKNTTSHDCKRINLTDGLSIDVNKTSKLCRNNSSDNLKINCQNKNNSFERNCVFLSTLNECEDYNCSVTSGNQKRTITINVNPDLDTFQLQSAQNGTTITLEWEKVTQNCAGLKFEYTCDGTHNSSTENESKHIFEKLTPYKTYTCEANAKYKNISTRKTKIITTDCDINMTLNLTAEEKSIDLVWEIKTEKQCNSVEFQCIYFCSRDDKKKDQPSTNLTILEGKAEHKATESNLQPYTNYTCHVIALYNNQQKKNVSMRNQTKIGTPQKVQNIQGKVNPHNTVKITCDKPVTFNGPSGFFKAQIKDKDYTAQKSKNCDFVFDDLDYLTNYTFVIWANNTKNEGQKSFYSIHTKYNDKALIGFLAFLIIVTSVALLFVLYKIYLLQKRNSNNNEETLELITPEDEAQLLNVEPISAEHLLDTYKRKVADEGRLFLAEFQSIPRVFSKFAVKDARKACNQNKNRYVDILPYDYNRVQLSMINGEVGSDYINASFIDGYKEPRKYIAAQGPKDETVNDFWRMIWEQQCSIIVMVTRCEEGNRNKCAQYWPSMDREAEIFGDFAVKINGENQCPDYIIRNLNVTNRKEKNSERAVTHIQFTSWPDHGVPGDPHLLLKLRRRVNAFNNFFSGPIVVHCSAGVGRTGTYIGIDAMMEGLEAEGRVDIYGYVVKLRRQRCLMVQVEAQYILIHQALIEHNQFGETEIPLSELHSTLNNLKKKDPPSDPTLLEGEFQRLPKYRNWRVQNTGTNEENKKKNRYSNAIPYDYNRVPIKLEDEKSQESEHEEDTEDSSDDDDEDSTKYINASFMDGYWGQRSLIAAQGPLPDTIADFWQMVFQKKIKIIVMLTECTEGDKDFCSKYWHDEKKLYDEIEVGVVDYNVCPEYIIRSIEIKHTKRKESRRIYQYHFQKWSGRDLPENPQDLIALIRSIKQKIPQKSGKDNKSVPVVVHCNDGSTRTGTFCALWNILDSADTENLVDVFQVVKNLRRERQGMLSSFEHYQFLYDAIASAYPAQNGEVKPARAADSVEVVNEGKKESVEEPVPQSTEKPQKASSKESEPADSTSASTEGKKKPSSSQEVPKESSSTEKVCEGDSEDSTSNGPTPNVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM62</accession>
    <name>W5MM62_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629487.1">
        <property type="nucleotide sequence ID" value="XM_006629424.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM62"/>
    <dbReference type="FunCoup" id="W5MM62">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009471"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009482.1">
        <property type="protein sequence ID" value="ENSLOCP00000009471.1"/>
        <property type="gene ID" value="ENSLOCG00000007804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694406"/>
    <dbReference type="KEGG" id="loc:102694406"/>
    <dbReference type="CTD" id="7480"/>
    <dbReference type="eggNOG" id="KOG3913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160653"/>
    <dbReference type="HOGENOM" id="CLU_033039_1_3_1"/>
    <dbReference type="InParanoid" id="W5MM62"/>
    <dbReference type="OMA" id="INAHNRN"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007804">
        <property type="expression patterns" value="Expressed in zone of skin and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013302">
        <property type="entry name" value="Wnt10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF76">
        <property type="entry name" value="PROTEIN WNT-10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01893">
        <property type="entry name" value="WNT10PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866302" description="Protein Wnt" evidence="12">
        <location>
            <begin position="29"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="405" mass="45267" checksum="DE223A7053A89F9F" modified="2014-04-16" version="1">MGIPHGLSWGRVLILAGLLRSPACTVLGNDILGLKVPRDPVLTPNTVCLALPGLSKRQMSLCVRSPDVTASALQGIRIALHECRHQLRDQRWNCSSLESQGKLPHQSAILRRGFRESAFTLSLLAAGVAHSVASACSLGKLRGCGCEAKRRLDDDKIRLKLTQLQLQTLQRTPGLRGPQPASHVPLHPSALLSPLPEDAPSLQDTWEWGGCSHDTRFGERFSRDWLDSRESPRDIHARMKIHNNRVGRQVVTDNMKRKCKCHGTSGSCQFKTCWYVTPEFRLVGALLREKFHSAVFINSQNKNTGVFNPWARGAGGRRRVSRELVYFEKSPDFCEREPALDSLGTQGRICNKSSGGMDSCSSLCCGRGHNILKQTRSERCHCRFHWCCYVMCEECRVTEWVNVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMC2</accession>
    <name>W5MMC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 51</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMC2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009531"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009542.1">
        <property type="protein sequence ID" value="ENSLOCP00000009531.1"/>
        <property type="gene ID" value="ENSLOCG00000007850.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047925"/>
    <dbReference type="HOGENOM" id="CLU_095080_1_1_1"/>
    <dbReference type="InParanoid" id="W5MMC2"/>
    <dbReference type="OMA" id="LWHQSNS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007850">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="6">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21316" checksum="815A9AF59CCCD1CB" modified="2014-04-16" version="1">FTGAPKGRKMYGPPVDFSFKCLSSLADVLSEHPNVGLKPVHRTPEGKFCSKALRLSNNILSNLSDFATASTALLDDPKQLSWIDLSFNDLSTIDPILTEFRELRVLYLHGNSISRLSEVDKLAALPHLQSLTLHGNTLENEKDYRNYVISALPHLKSLDFSAVTKQDRVTAQIWRRGSSRPKRSRKTPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNX7</accession>
    <name>W5MNX7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metaxin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial outer membrane import complex protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in transport of proteins into the mitochondrion. Essential for embryonic development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with MTX2/metaxin-2. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with ARMC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNX7"/>
    <dbReference type="FunCoup" id="W5MNX7">
        <property type="interactions" value="1729"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010098.1">
        <property type="protein sequence ID" value="ENSLOCP00000010086.1"/>
        <property type="gene ID" value="ENSLOCG00000008303.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182919"/>
    <dbReference type="HOGENOM" id="CLU_044137_5_1_1"/>
    <dbReference type="InParanoid" id="W5MNX7"/>
    <dbReference type="OMA" id="FPYNLYY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008303">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03212">
        <property type="entry name" value="GST_C_Metaxin1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF34">
        <property type="entry name" value="METAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="16">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="17">
        <location>
            <begin position="126"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="230" mass="26032" checksum="55B710F46EC5DCEA" modified="2014-04-16" version="1">MAATMELYCWKGDWGLPSVNTECLTVLKYNADYDLSAKEGADTLAFVSLLEQKLLPALIYAQWVDSKNYVDVTRRWYAENIPFPLNFYLPNRMHARQLERLRLVTGDSGLEPGEEIEKELYRDALECMTLLSQRLGQHKFFFGDSPSSLDAYVFGHLAPLLKAKLPNSKLQQHLQSLDNLCQFCTSILQLYFPSEGSGEPAGPPFPILFLLFRAGAFPLVTCVQHSALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPE4</accession>
    <name>W5MPE4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5MPE4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010253"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010266.1">
        <property type="protein sequence ID" value="ENSLOCP00000010253.1"/>
        <property type="gene ID" value="ENSLOCG00000008433.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204465"/>
    <dbReference type="HOGENOM" id="CLU_332512_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPE4"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="425"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="3">
        <location>
            <begin position="738"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="861" mass="100433" checksum="F15632FCC24178B8" modified="2014-04-16" version="1">QVTPTWFSDHRLLTVSFGVEDPRFQAGYWKLNKQVLHEERYRRKIEAALKNLEKRRELFSSAVHWWESCKQTLAMVTRRYCWIRRSLEERQCAQLQEELEALYEQLNQGGAFNGEKIANVRSLLREKWLQRAKNFLFRLRGVWKEGEEQCSPQFFSAERKQQERCCFESIRREDGTAVTDLPGMLECATGFYTALFQEKEIDASIKERLLEGVEKAVDDPASLVGPVTGAEIKTALFGLKKGTVPGVDGLPAEFYRTFWDQLEPFLVQVFNEALDSNELINSFVVIPGRVLVVDGQIGRDSLRLINVYAPAQRRLRKDFFTVLEPLLMTNKKIILGDWWEDVKRRLKRETIRYCRRRRKKKRLKNFSFDCSEGNLSCLFKMVRKDQKKMVFDGIQRKDGGVTMGAREMVEEATKFYKEFFREEPVSALLYVIYLEPLLEMIRKHKEINGIMIPGSRGQQAKLAAYADDVVLMLDSDMGLLRAIEACNSFGQVTGAKINTTKSKMKYFGIWKTRKETIGGLTLVPGPIKILGVNFAIRGSGHLNWKEKINFLKIKLGRWKNVQLSYSSKIMVVKSDGLSALVFLAYVFPLPPSLRKEVIRSVFTFIWSNKNEYIRRVDMYRKIEEGGRDVPNIPLKLDAIFVSCLCRALKTDVMHKSQYFVRLWFSWTMPRLRPISNTGPKADVTPWYYNHAGKWLKNHPEGKDEIFVIQQKALYNLVNEQPGMALIGKKKENWIRVQPKELDYSYKDLNWLCVWNRLPVREVMFRHKLTKTKHCPYSECEETETIKHVFWECVTAKQVWDKCQQFLTCIQPGFLLTSEKVTFAWKGTERKGTVDWVLWLVISIIKHFLWSARCAQIRDGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPH7</accession>
    <name>W5MPH7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proline-rich coiled-coil 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRRC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002746">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPH7"/>
    <dbReference type="FunCoup" id="W5MPH7">
        <property type="interactions" value="722"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010286"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010299.1">
        <property type="protein sequence ID" value="ENSLOCP00000010286.1"/>
        <property type="gene ID" value="ENSLOCG00000008454.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUZV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003837"/>
    <dbReference type="HOGENOM" id="CLU_041959_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPH7"/>
    <dbReference type="OMA" id="ELFPDQW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008454">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010669">
        <property type="term" value="P:epithelial structure maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001757">
        <property type="term" value="P:somite specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="PRRC1 isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
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    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
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        <property type="entry name" value="PRRC1"/>
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    <dbReference type="PANTHER" id="PTHR23276">
        <property type="entry name" value="PROTEIN PRRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23276:SF2">
        <property type="entry name" value="PROTEIN PRRC1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-canonical purine NTP phosphatase/PRRC1" evidence="5">
        <location>
            <begin position="260"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="441" mass="46563" checksum="9151F3EC25705D92" modified="2014-04-16" version="1">MMEESGIETTPPATPPPPSTAVVGPAPPALSLGMSSSLTLPSTTGISSYPPQGFSTPPASAPPIRSSLPPSFGPMTSASPLPPPGTAPYSSPLAAYPAGPAPPLSTAPPPGPVISAPPQAPPTSGFSMGASYDITRGHAGRTPQTPLMPTFSSPPVVTDITDPNIYCLRISPYMSNAETNTGPDIKFTSEHVCPSYTLTPLPEPVVSGNSSFIKGVAGNPMVKSVLDKTKHSVESMITTLDPGMAPYIKSSGDLDIVVTSTKEVKVAAVRDAFQEVFGLAVVTGEASQSNIAPQPVGYAAGLKGAQERIDSMRRVGMIHEKQPVVSVENFIAELFPDKWFDIGCLILEDPGHGIHIESFTQATPVPLEYVQQAQSLTPPDYNLRWSGLLVTVGEVLERNLPHISRTDWHVMLTGMSRRQMIYSAAKALAGMYKQHLPPRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUB3</accession>
    <name>W5MUB3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="4 15">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="4 15"/>
    <dbReference type="EMBL" id="AHAT01016458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016460">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUB3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011972"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011993.1">
        <property type="protein sequence ID" value="ENSLOCP00000011972.1"/>
        <property type="gene ID" value="ENSLOCG00000009793.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158216"/>
    <dbReference type="HOGENOM" id="CLU_009311_2_1_1"/>
    <dbReference type="InParanoid" id="W5MUB3"/>
    <dbReference type="OMA" id="CAEDRGY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009793">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14059">
        <property type="entry name" value="STKc_MAP3K12_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000087">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000095">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017419">
        <property type="entry name" value="MAP3K12_MAP3K13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF14">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038165">
        <property type="entry name" value="MAPKKK12_MAPKKK13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="196"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="563"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="743"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="810"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="202"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038165-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR038165-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="960" mass="106630" checksum="5DBCA092B71B9C5A" modified="2014-04-16" version="1">MHTHSTMGSPPEVLAWTSSPSLLVGKLKDELLSTGNPPNKMTATPPSPPPPQIIEDLPPLQQPLEEELVLGSSPPNTVLTSVSEDSSREQRHFDNSVLQLQENEEVDTTAPQGQGCGSGGGDGGGCPADHSGSSGVQTHAHDDIKMHFQRTGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDTWEVPFEEISELQWLGSGAQGAVFLGKFHSEEVAIKKVREQKETDIKHLRKLKHPNIIAFKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKISPRLLVDWAYSSELHLNFVHINSIVKRDLGDVSVLVTHNDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQGKPRNRPSFRQILLHLDIASADVLGTPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRRDELRHALDIREHYEKKLERANNLYMELSAIMLQLEVREKELIKREQAVEKKYPGTYKRHPVRPMVRPNAVEKLIKKKGSMAHKPGVQAAKRPDLLRSDGIPSVEVLPSPSPLSGSPKVSTPPGKARYRSKPRHRRGNSKGSHSEFSGILKNVAAAPEEQQPLQHYHQHHHHHAREDAVANCANNLRYFGPAAALRSPQTDHLQRRVSGSSPDLISTAMEADSRERATPDRSGPGSLCPCCQAHPFPGCPHCQETAGERFPAPSHPKRPSYSLLSTYESHPQQAEHGLPASKDKDGVPHDNSPHDITPPGLPRSLRPLRKDGDDSSEEEEGEVDSEVEFPRRQRPHRCMSSFQSYSTFSSENLSVSDGEEGNTSDHSHSGPLEQLSNSQEERLDELLSQTPELPIDISTQSDGLSDKECAVRRVKTQISLGKLCTDEHSYENPLHYGESDCDSSEAECSDATVRNNKPCGTSTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW52</accession>
    <name>W5MW52_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MAGUK p55 scaffold protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW52"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012611"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012635.1">
        <property type="protein sequence ID" value="ENSLOCP00000012611.1"/>
        <property type="gene ID" value="ENSLOCG00000010293.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156444"/>
    <dbReference type="HOGENOM" id="CLU_001715_5_0_1"/>
    <dbReference type="InParanoid" id="W5MW52"/>
    <dbReference type="OMA" id="PACWIRP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010293">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="7">
        <location>
            <begin position="60"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="134"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="222"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="5">
        <location>
            <begin position="403"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="612" mass="69832" checksum="8F5F1443B19D2A0E" modified="2014-04-16" version="1">IALCPLGLSEILTNVVEEVKVSISRDINGADILYSLLNAPWLQSLLKVYECLQQYLQRNPSPFLPYASGLSQEVMSDLRMSASSPDARELLILLRNPHMQAVLSAHDTVAQKDFEPVLPPLPDNIPEEEEAMRIVCLVKNKQPLGATIKRNEITGEIVVARVIHGGLADRSGLLYAGDKLVEVNGHSVEGLEPEQIIQILAQSHGTIVFKLVPISERPVNSQTMMYVRAMTDYSPQQDPAIPCADAGMAFRKGDILEIVDQTDFLWWQARKLPNTSCCAGLIPSANLLKRRKQREFWWSQPLQPHTCVKISPELTHKFKEDMMAIDEKCVETEELREEEDGFSALNQGLYLAGFRRSLRLCRRKSRSSHQQSCYARCPSSCYSALASPYEEVVRYQRHPEDRHRLIVLIGPSGVGVNELRRRLIEINPKIFQGAVPHTTRPPKSYEENGREYHFVTKELFENMIHSNRFLEYGEYKGHLYGTSIDSVKEVLENGKICIIDLEPQGIQGVRTHELKAYIIFIKPSSIIRMKQTRKSARIITDYYVNRPFKEEDFQEMEEMAKKVEAQYCQFFDHVIVNDSLQDSCVQLLTAVRRAQDEPQWVPALWLRPAEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWP1</accession>
    <name>W5MWP1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01013285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012800"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012825.1">
        <property type="protein sequence ID" value="ENSLOCP00000012800.1"/>
        <property type="gene ID" value="ENSLOCG00000010445.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690523"/>
    <dbReference type="KEGG" id="loc:102690523"/>
    <dbReference type="CTD" id="9867"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154585"/>
    <dbReference type="HOGENOM" id="CLU_026830_1_0_1"/>
    <dbReference type="InParanoid" id="W5MWP1"/>
    <dbReference type="OMA" id="NHSEGEC"/>
    <dbReference type="OrthoDB" id="21204at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010445">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16465">
        <property type="entry name" value="RING-H2_PJA1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000152">
        <property type="entry name" value="E3 ubiquitin-protein ligase Praja-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="628"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="121"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="423"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="702" mass="78499" checksum="127C2A19DFE3B4AE" modified="2014-04-16" version="1">MGQEAGKPAWPKPAGGYQTITGRRYGRRHAYIGFRPTLAKQNIPSPENDMEWQGLEMSSVHEENPLSSNGQITSTLQAILPVESNSVHPEASGTNFFKDVPRVKHVASQKGNEATSFSYKKLKSDCDDPNSKKHLGPKKSSEACAWPTFEDQKERSRSPIGLSFVNIDSYEPDSSDGEEDELSGNSSIEAHPGLQKRLDNMICELEKEFDYLSGLHSYLCTKTCENHEPLPKSSCPEKDIEEPQQNSFVSQKSNSSLREESINLSNICNDRQKKHEDSEINVSLVSECTPQKSFDWNNDQGVSQTEMVVRPKIRKQSTDSHLEKSKGSQLDDGQHAFCRKTQAANNTWSSIPPFFLTQTEKPEKEFLYENSCTDSAPDELRPFEGKQERMEMHNRNKHDEEDDDLWEDLEDFGEKCASFIKDDNSSECSEGEWSATCTSESGVDKQQCSSGESWETLPGLDEPEHDLQSNSSSLDDIPDISFNPEGPMLLEEGEIPWLPYQEETDSSSDEETDGMSHFVHPGLFMLDGNNNFEDDSSMSEDFDAEWRLLDEFGEGFGVAQAISYVDPQLLTYMALEERLAQAMEAALAHLESLAIDVEQAHPPATEEIIDCLPQITVLEDHHGQEQCCAICCCEYIKDEIATELPCHHVFHKTCVSLWLQKSGTCPVCRHVLSPALPEAAVATSFVSDHDTPPSTHSAAGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXA0</accession>
    <name>W5MXA0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin-13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXA0"/>
    <dbReference type="FunCoup" id="W5MXA0">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013009"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013037.1">
        <property type="protein sequence ID" value="ENSLOCP00000013009.1"/>
        <property type="gene ID" value="ENSLOCG00000010620.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063347"/>
    <dbReference type="HOGENOM" id="CLU_005996_3_0_1"/>
    <dbReference type="InParanoid" id="W5MXA0"/>
    <dbReference type="OMA" id="KYPAEMA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010620">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007634">
        <property type="term" value="P:optokinetic behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000107">
        <property type="entry name" value="Importin-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040944">
        <property type="entry name" value="Importin_rep_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040520">
        <property type="entry name" value="Importin_rep_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18786">
        <property type="entry name" value="Importin_rep_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18806">
        <property type="entry name" value="Importin_rep_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="962" mass="107226" checksum="A2899E39D086E08B" modified="2014-04-16" version="1">AGMEPGRPAHSPDSLEFTVENVEKALHQLYYDPNIENKNLAQKWLMQAQVSPQAWQFCWVLLSPEKVQEIQYFGASALHTKISRYWGDIPAEQYDTLKTQLFGQITRFASGPKIVLTRLCVALASLALNMMPEAWPAAVSEMVRVFQAEGAGVDGRARCLALLELLTVLPEEFQTSRLPQFRRGQVRGALGQEWAAVFPLLQQLLRQADSPGFIKAKVLKCLSSWVLLDVPLNESEGLLQECFAALCDPELFDTAVEAIVNAISQPDSQRYVNTLLKLVPQVLQLQEQLRQAVQSGDMETSHGICRIAVALGENHSRALLEQVEQWQGFLALVNMIMFCTGIPGHYPVNETTSSLTLTFWYTLQVGIVSWEFQAITAPCKLQSKPCPYLYLVSILVFLEQYTDVKNSLMDLSRSREQAHSTGVDISDTLMYVFEMLGVELLSNLYDKLGRLLTNTEQPSSWQHTEALLYGFQSIAETIDVNYSDVIPGLIGLIPRISINNVQLADTVMFTIGALAEWLADHPVMLSSVLPLVLHALGNPELSVSSVSTLKKICRECKHDLPPYAANIVAVSQEVLIKQIHKTSQCMWLMQALGFLLSALPVEEILRNLHSLITPYIQQLEKLADETPNPSNKLAIIHILGLLSNLFTTLDVTRQEEEGEGAPPVKTAPPEPGPNPVVVVLQQVFALIQTVLSKWLNDSQVVEAVCAIFEKSVKTLLHDFAPMVSQLSEMLGQMYSTIPQASALDLTRQMVHIFASETDHFPPIKALFELVTSVTLTLFQQGPRDHPDIVDSFMQLQAQALKRKPDLFLSENLDVKAVFHCGVLSLKFPEAPTVKSTCLFFTELLPHCADTPPIGQVVQEDGKLLLQAVLEAVGGQASRSLMDQFAEVLFALNKHCFNCLALWLKEALQAQGFPSSRVSTEQKDTFSQQILRERVNKRRVKEIVKEFTLLCRGLHGTEYAADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXL7</accession>
    <name>W5MXL7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Neurobeachin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat neurobeachin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MXL7">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000013154.1">
        <property type="protein sequence ID" value="ENSLOCP00000013126.1"/>
        <property type="gene ID" value="ENSLOCG00000010703.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155041"/>
    <dbReference type="HOGENOM" id="CLU_000218_0_1_1"/>
    <dbReference type="InParanoid" id="W5MXL7"/>
    <dbReference type="OMA" id="IVFKVME"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010703">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001375">
        <property type="entry name" value="Neurobeachin-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF115">
        <property type="entry name" value="NEUROBEACHIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16057">
        <property type="entry name" value="DUF4800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="10">
        <location>
            <begin position="1934"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="9">
        <location>
            <begin position="2042"/>
            <end position="2334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="2538"/>
            <end position="2579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1345"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1421"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1914"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1346"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1423"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2744" mass="309917" checksum="ADA523661D599311" modified="2014-04-16" version="1">YCCVNEMYFFSLQKDPDYLKLWLEIFVSSYEKCLDADFEKPPTRLEEIPPVMSLLPDNILQVMRHQLLQCVQKVSDGLEEEQQTLALLLVKFLIVICRNLSNVEEIGTCSYINHIITMTTLYIQQLKSKTKEKELADQTPAEEFVRHALAFCESLYDPYYNWRHRMSGVQVNTVEKSRQKYKAAPLTVEFIPFFYRKSCFYDSVNSQFLNWKVGNQFMFARDKRGTMVIVLKCRLKCLLNMILALLSSHSGGFVKGCVNEFQYTQKDKLGVGCLRNLPLHRVLRDSINPNTAQKTNLNEQRVLLMVRPTDANIKVKENTETGGSNSDYWHNTFTSLKENLPETIENQFISVATPGFQGIFGNRNQFERIIRRLVNQLVVNARCKAEVKSKKDFANKLLTEMNEYQVFQGRLDCLAVSSIKALTTVMLKSPAAKYIMLSLLQKFHLIEVLSSLGQPSRDLLKELMNMAVEGDPASVGILGISNVQPLLLLIQWLPHLESHDLQIFISDWLKRICCINRQSRTTCVNASMVIHILDTLNCHSRLHRTCAENLISLFGSLGSQSLSSKELLQLMRLLRTDDPKQAHPYVVPVLRAILGMARKQGLENALQYFDLSPSMAGISVPTVQRWPGYAFSFFAWLCLDQDQQGSGISSKGEKRKQLYSFFTASGTGFEAFISSSGVLVVAVCTKKEYTTVMLPDHCFCDSLWHSIAVVHIPGKRPFGQSLIYIYVNGQQKLSAPLKFPTMTEPFISCCIGSAGHRTTTPPPSQIPDPPFSHLSAPGRSSLGGILSAPTWGGLLGKPESVTKLISAGTQDSEWGSPTSLEGQLGSVMVFHDALQPAQVKLLFAAGPNCISPFKSQESELGDLPSKLLLYYSPKACKNPICLDLSPNHFNGRLTGNKVVNWDIKDMINCIGGLNVLYPLLEQISNLSGAVKSEDASGSEYITTELCTPSDGDWVILPSTKAPEARLEKNVVATFLLMIKHFIQRHPINQENLIHSHGVATLGALLQKLPSSLVDVNVLVAVQLLTEQVSIETNPQLLQQMYHHLLFNFNIWNQGDFPLRIGHIQYMSTIIKDSRKQFRKKYGVQFLLDTIRLYYGTQSKRSDLSEDDMKTIRASLCGLLKYYISKGVVQEELQSILGYIAAIGDEEQLCGILEVLLNLLQTSAARDQLFLLLFEKAFKKVSHASLNSLNLMLGQQMTHVYLVRRQPEYSEVDNRRNEIRFRRGQIYSNEIPITVSLIKNLLNQVLTSDPVVNYKDLMSVVHLTHKAGLNIRLLVCKKLYQLLQSQQDAAQQISRQLGWQETLVKLFLRDSSEAQEGSNSSSNKADTASTSSLDFTKNRVSVSSSLLEKKGSGDVHGRLEDDKNGVEGHSLADNKSIDSLSQSLDNGDLDSLDTPAEATTKPWASKAGGLSLDLSRTHLYEQVDSGSQTPGSMPSTPSPLETSRPFPGLQAERETSSFMDDSFIFTDDFSLNESFASAERVEEELTGMLTEILLCVMWRGLDGSDDAAWLDRGQVFSALTKMGTTNELLQPADEIKLSLLEKMLEWAVTDNREATAATLPVHTENAVRLLHIVQDFLQAEGLVNPAMWTEKVLEEAVTLMDSLMVWYSSGTQWMQLSQVGIRLLLGFMAQENLQVCAMATAKLNTILQTKAIESQDEACYLLGKLEGILSKSIREQTETYSFLIPLIRTLLSKIYKLLYMELHLPSLPDTNGSPSFFEDFQVYCSSEEWRVYLDKYIIPYMKQYEIEMFSRGHEAMAVYWKDCYEAFMVNLHKREREKGESKLKFQELFVEPFTRKARQENLRYNSMLKLINNQQNTVLRQWKAARRGLVCERGPWAHRQQSKMHWKVSSAENYSRMRLKLVQNYNFDPHREASALRDNLDSPGTGVQLYQPTTESLLLEAVKQAKVNDLEDDMLELPEDDPTGGNNLNETEEQSQKEKLVISEDCELITVMDVISGRLEVTTQHIYFYDGSVEKEEGVGYDFKWPLLQIREVHLRRFNLRRSALEIFLIDQTNYFLNFKKEVRNKVYSRILSLRSPNLYGTRSPQELLKASGLTQKWVNREITNFDYLIQLNTIAGRTYNDLAQYPVFPWILSDYTSEELDLNNPLVFRDLSKPIGVVNEKNAKSVREKYESFEDPTGTIDKFHYGTHYSNAAGVMHYLIRVEPFTSLHIQLQSGRFDCADRQFHSIPATWQALVDNPNDVKELIPEFFYFPEFLENQNEFDLGCLQISKERVNDVILPKWARGPEDFIYKHRKALESEYVSAHLHEWIDLIFGYKQRGPAAVEALNVFYYCTYEGAVDLDAVADEKERKALEGMISNFGQTPCQLLKEPHPVRLSAEEVEKRKARQDTCPLSIFEHLPELKSFFVEGISDNIPIVKAVVPKNQSRSFISQGSPDNLITVSQNCLIGTHGWLPYDKNISNYFTFIKDPTVSNSKTQRFINGPLSPGLEITAKLFVMSHDGKLLFSGGHWDNSLRVTSLTKGKLVGQHIRHMDIVTCLSSDYCGIHLISGSRDTTCMVWQILHQGGVPVGLSPKPVQILYGHTNEIVSVSISTELDMAISGSTDGTVVIHTIRRGQYMRSLRPPCESSLPVTILNLAISWEGHLVVYTSIEGKSTLKDKNALHLYSVNGKHLCSETLKEQVTDMCVSGEYIVLGSAQGYLSIRDLYSLCLCTAPMAMRVPVHCISVSKENSHALVGLEDGKLIIVGVGKPAEMRSGQITRKLWGSSKRLTQISSGETEYNTQDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY46</accession>
    <name>W5MY46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY46"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013333.1">
        <property type="protein sequence ID" value="ENSLOCP00000013305.1"/>
        <property type="gene ID" value="ENSLOCG00000010855.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW4X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5MY46"/>
    <dbReference type="OMA" id="YSINWFL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
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        <location>
            <begin position="49"/>
            <end position="300"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    <sequence length="336" mass="38436" checksum="4D5CC62D02D583A8" modified="2014-04-16" version="1">MTSQMSTTPVLNTTFIRPYGFYIRGFYDLENSNYYLIFLGVIYVATLLANALIMSIIWFAQPLHTPKYFGVFSLALVDVSYSTSLIPKSLDMFIYGNRLVEYNTCLTQMFFVQYFSAMESYALIVLAYDRFIAICFPLRCQTINSNTKMIIVIIISWFIPFIVVLTMVCFVTRLSYCKSVIVNSFFCDHGPVFKISCSDYSINWFLASFFVVSLLFFPLALILLSYVCIVVALLKIAEIDSRRKAFKTCTSHLILVAIFYVPLLVTNIIAWVNVNIDTNTRILNTSLSVSIPPLLNPIIYTLKTEEIMVQIKKYIRNSGDKIGNNHQASDSTLMYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY89</accession>
    <name>W5MY89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="17">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the RNA polymerase I (Pol I) complex consisting of 13 subunits: a ten-subunit catalytic core composed of POLR1A/RPA1, POLR1B/RPA2, POLR1C/RPAC1, POLR1D/RPAC2, POLR1H/RPA12, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk subunit POLR1F/RPA43 protruding from the core and additional subunits homologous to general transcription factors POLR1E/RPA49 and POLR1G/RPA34. Part of Pol I pre-initiation complex (PIC), in which Pol I core assembles with RRN3 and promoter-bound UTBF and SL1/TIF-IB complex. Interacts (via dock II domain) with TOP2A; this interaction may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with CAVIN1; this interaction induces the dissociation of Pol I complex paused at rDNA terminator sequences. Interacts with MYO1C. Interacts with ERBB2. Interacts with DDX11. Interacts with RECQL5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the RNA polymerase beta' chain family.</text>
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        <property type="gene ID" value="ENSLOCG00000010881.1"/>
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    <dbReference type="GeneTree" id="ENSGT00920000149138"/>
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        <property type="component" value="Linkage group LG4"/>
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        <property type="term" value="C:chromosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:neural crest cell development"/>
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        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
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        <property type="entry name" value="RNA polymerase II, clamp domain"/>
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        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
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        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
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        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
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        <property type="entry name" value="RNA_pol_asu"/>
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        <property type="entry name" value="RNA_pol_N"/>
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        <property type="entry name" value="RNA_pol_Rpb1_1"/>
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        <property type="entry name" value="RNA_pol_Rpb1_3"/>
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        <property type="entry name" value="RNA_pol_Rpb1_5"/>
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        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb1_1"/>
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb1_2"/>
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        <property type="entry name" value="RNA_pol_Rpb1_3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05000">
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb1_5"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 17" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="19">
        <location>
            <begin position="297"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1366"/>
            <end position="1487"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
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            <begin position="1372"/>
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            <begin position="1413"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1423"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1433"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065144"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004279"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00663"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013348.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1712" mass="191979" checksum="340874B9680BA641" modified="2014-04-16" version="1">MLFAKDAPWRRLEGMSFGIYSTDEIRKLSVKTITNPRFLDNVGNPATNGLYDLALGPADSKEVCTTCMQDFNKCPGHLGHIELPLPVYNPMFFDKSTLLCLGSSFSCHMLTCSRAAVHLLLNQLRVLEAGALNAVYDLEAELNRYLEGNVSASGVEIQEVLEDYTKKILRTAIGYSSPVKNVCEIKNNLISNFWKLHVKSRKCPNCKTGRSVVRKEHNSKLIVTLPSSVHKASKTGEANQKETEVINEAQLGKRGYLTPSTTKEHVLRLWENEGFFLKYLFSGLEVYENSKSCFNPDVFFLENLVVPPSRYRPINRLGDQMFTNGQTVNLQAVMKDNTVIRKLLALMAQEKEKALEHPETETAEVSVVPALEVESNQTDRAFLSSIPGQTLTDKLYNVWIHLQSHVNIVFDSDMDKLMGDKFPGIRQMLEKKEGLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKELRQAVINGPDVHPGASMVISEDGSRTVLSSTNRTQREAIAKQLLTPCTGPQRMSMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYNLVSTDQQYLVPKDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQYMELVYRGLTDKRGRVRVLPPAVLKPCALWTGKQVVSTLLINVIPEKHLPLNLIGKAKIPSKAWIKEPPRPVPGYNPDSMCESQVIIQDGELLCGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAHLQLYRGFTMAGVEDILVKPGAKSTECGAKAVRAAFNLPETVPESEARDKWHDAHLSPDQRDFNMVDLKFKEQANLVSNGINKVCMPLGLHRRFPENNLQLMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQPYDVSPRAGGFVSGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPRQFPFIRDNYEVIKKSKCLDEALSKMDSEASSKHFRAIQKWKTKHENSSPRQGAFLLFSQKKLAKLQAHCEQGSLVNSRTLATQQLIERWYSLDKEGHRKYARKTERCPDPCLAVHRPDISFGSVSETFESIVDSYLSDYQAQQSSADCEKSLSVESLKSLLHLKWQRALCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASSSIKTPMMSIPVLNTKKALKKVKKLRKKLTRVCLSEVLQRVDVKESLSVQEKLKKSRVYQVTFHFLPPERYQHDKPLSPKQILHFMETRFFRILLEAIRKRSLKLASLNSVNTSKATQRDLHDSEEPQKSHSVLKSGVNKAEDDREGEGEEDARIVDAEADEGDADATDTRRRQKQEEEVDYDSEEEGDNDEANELQEEEEGPEGRQENEEEMETGQVPPQSTAALPAAKNTELDEVRINTVLGTSSSIESYRYDTEQGLWCEVTLNLPVTKVYFDLTSLIASQAQSAVVHETKGITRCLLNESTNSRGEKELVLNTEGINLTEVCKYSDILDLKRLYCNDVHAMANTYGIEVALRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGIKSNSSPLQQMTFETSFKFLKDATMLGAYDELKSPSACLVVGKVVKGGTGLFDLKQPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYP8</accession>
    <name>W5MYP8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial enolase superfamily member 1</fullName>
            <ecNumber evidence="3">4.2.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-fuconate dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:22772"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:21291"/>
            <dbReference type="ChEBI" id="CHEBI:37448"/>
            <dbReference type="EC" id="4.2.1.68"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.68" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01017628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYP8"/>
    <dbReference type="FunCoup" id="W5MYP8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013507"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013536.1">
        <property type="protein sequence ID" value="ENSLOCP00000013507.1"/>
        <property type="gene ID" value="ENSLOCG00000011003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690820"/>
    <dbReference type="KEGG" id="loc:102690820"/>
    <dbReference type="CTD" id="55556"/>
    <dbReference type="eggNOG" id="ENOG502QU7C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014290"/>
    <dbReference type="HOGENOM" id="CLU_030273_2_0_1"/>
    <dbReference type="InParanoid" id="W5MYP8"/>
    <dbReference type="OMA" id="SGAIDVC"/>
    <dbReference type="OrthoDB" id="14161at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011003">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050023">
        <property type="term" value="F:L-fuconate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03324">
        <property type="entry name" value="rTSbeta_L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000007">
        <property type="entry name" value="Mitochondrial enolase superfamily member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034610">
        <property type="entry name" value="L-fuconate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00111">
        <property type="entry name" value="L-fuconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="11">
        <location>
            <begin position="199"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="442" mass="49655" checksum="3FA8F36AFDF6AB01" modified="2014-04-16" version="1">MVKGKITKLSVSDVRFPTSLEQHGSDAMHTDPDYSVAYVVIETEGGLKGYGLTFTLGKGTEIVVCAVQALSSLVIGKDSEEIVNDFRSFYRLLTSDGQMRWLGPEKGVVQLAAAAVLNAVWDLWARAEGKPLWKLLVDMDPEKLISCIDFRYITDALTEKEALDILRKARDGKCKREEHMLKEGYPAYTTSCAWIGYPDEQLKQLCSKALSEGWTRFKVKVGADLNDDIRRCRLLREMIGPDKTLMIDANQRWDVAEAIEWVSRLVEFKPLWIEEPTSPDDILGHAAIAKALAPLGIGVATGEQCHNRVMFKQLLQSQALRFVQIDSCRLGSVNENLAVLLMAEKFQVPVCPHAGGVGLCELVQHLILFDYIAVSGSLENRMCEYVDHLHEHFTNPVVIKNASYMPPKNPGYSTEMKDDSAKRHQFPDGEVWRELRASRRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYX3</accession>
    <name>W5MYX3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone-lysine N-methyltransferase SETMAR</fullName>
            <ecNumber evidence="12">2.1.1.357</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60308"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.357"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.357" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01031664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYX3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013582"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013611.1">
        <property type="protein sequence ID" value="ENSLOCP00000013582.1"/>
        <property type="gene ID" value="ENSLOCG00000011063.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698683"/>
    <dbReference type="KEGG" id="loc:102698683"/>
    <dbReference type="CTD" id="6419"/>
    <dbReference type="eggNOG" id="KOG1082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162663"/>
    <dbReference type="HOGENOM" id="CLU_020840_3_3_1"/>
    <dbReference type="InParanoid" id="W5MYX3"/>
    <dbReference type="OMA" id="VDSMVPK"/>
    <dbReference type="OrthoDB" id="616263at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011063">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140954">
        <property type="term" value="F:histone H3K36 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="296" mass="32781" checksum="E8D0BD22342E5DCC" modified="2014-04-16" version="1">MSWNSEYDVCCGLENLPVFVENKINKEDLPQFQYSPEQVPGPGTDTDPSEVTFPGCGCLATSCLSGSCTCLERHGQMYDGNLRLRDQEENEEHSRPVFECNVMCRCSENCANRVVQRGLGFRLEVCQTHTKGWGLRTLEPIQKGRFVCEYAGEVIGFAEACRRQASQKPEDMNYIIAVKEHTGGSGTIETFVDPARVGNVGRFLNHSCQPNLFMVPVRVDSLVPRLALFAGRDIASGEELVFDYSGRYKNSLGNGQAPNTISAESWMRKPCYCGSQDCCGFLPLDISVINNGEVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZX4</accession>
    <name>W5MZX4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Survival of motor neuron 1, telomeric</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Gem</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMN family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015338737.2"/>
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    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000011339.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="loc:102694198"/>
    <dbReference type="CTD" id="6606"/>
    <dbReference type="eggNOG" id="KOG4327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153352"/>
    <dbReference type="InParanoid" id="W5MZX4"/>
    <dbReference type="OMA" id="DETVNGM"/>
    <dbReference type="OrthoDB" id="197400at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011339">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097504">
        <property type="term" value="C:Gemini of Cajal bodies"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:axon arborization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of axon extension involved in axon guidance"/>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:spliceosomal snRNP assembly"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22852">
        <property type="entry name" value="SMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMN_N"/>
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    </dbReference>
    <dbReference type="CDD" id="cd20398">
        <property type="entry name" value="Tudor_SMN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040424">
        <property type="entry name" value="Smn1"/>
    </dbReference>
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        <property type="entry name" value="SMN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049481">
        <property type="entry name" value="SMN_G2-BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010304">
        <property type="entry name" value="SMN_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047298">
        <property type="entry name" value="Tudor_SMN_eumet"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267:SF1">
        <property type="entry name" value="SURVIVAL MOTOR NEURON PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267">
        <property type="entry name" value="SURVIVAL MOTOR NEURON-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20636">
        <property type="entry name" value="SMN_G2-BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06003">
        <property type="entry name" value="SMN_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20635">
        <property type="entry name" value="SMN_YG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="12">
        <location>
            <begin position="82"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013933.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="279" mass="30461" checksum="F780D866BED30F19" modified="2014-04-16" version="1">MANGYKEVLFTRGIGQSDDSDIWDDTALIKAYDKAVASFKNALKSEDNTELPKKGKSPAGKKRKSNKKSKSRKKNNAIPDKEWKIGDSCISVWSEDGIAYPATIKSVDHEKGTCIVVYTDYGNEEEQSLLDLQSQNSEEEVEMGNAAQENDPEFSTDDSDRSSTPHLPSQAKPKARSPPGPPLWNRGFPPPPPPMPGFGMAEPRIDGPAAAFPGWPPLIPGGPPLIPPPPPLGPDFTEDYEALGSMLIAWYMSGYHTGYYLGLKQSQRDSASGNKSQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0K3</accession>
    <name>W5N0K3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">G-protein coupled receptor 74</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0K3"/>
    <dbReference type="FunCoup" id="W5N0K3">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014162"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014191.1">
        <property type="protein sequence ID" value="ENSLOCP00000014162.1"/>
        <property type="gene ID" value="ENSLOCG00000011523.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286926"/>
    <dbReference type="InParanoid" id="W5N0K3"/>
    <dbReference type="OMA" id="TRPVYWC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011523">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15980">
        <property type="entry name" value="7tmA_NPFFR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000137">
        <property type="entry name" value="neuropeptide FF receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005397">
        <property type="entry name" value="NPFF_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF132">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01572">
        <property type="entry name" value="NPFFRECEPTR2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="65"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="429" mass="49112" checksum="BA623C315D58A5D4" modified="2014-04-16" version="1">SKMNTQLDPNISYIDRKSWHFENNSQEYFYPPYNTTYVGFYLHQPPVAAVFIVSYLLIFLVCMVGNGIVCFIVLRSKHMRTVTNLFILNLAVSDLLVGIFCMPTTLLDNIITGWPFGSLVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTSTLIIVIIWVLAISIMCPSGVMLQVTKEQNVRVLLGEGNKTNPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYARIGITLFKTAVPLGEKPGHENRQTGSKKKQRVIKMLLIVALLFILSWLPLWTLMMLSDYANLTEYQYRVINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAAFKFQLCSANIERRDTYSHKVQGNAVLPAGIQVNKDPISMNTNSIDNHSSKRNNNQNEQDLIMEDLEKVSNDNGIQRDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N123</accession>
    <name>W5N123_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N123"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014332"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014361.1">
        <property type="protein sequence ID" value="ENSLOCP00000014332.1"/>
        <property type="gene ID" value="ENSLOCG00000011665.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102697830"/>
    <dbReference type="eggNOG" id="KOG3587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155025"/>
    <dbReference type="HOGENOM" id="CLU_037794_5_0_1"/>
    <dbReference type="InParanoid" id="W5N123"/>
    <dbReference type="OMA" id="EIHNPLE"/>
    <dbReference type="OrthoDB" id="8918229at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011665">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000021">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF104">
        <property type="entry name" value="GALECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="134" mass="15342" checksum="EB9215DD3DEDF513" modified="2014-04-16" version="1">MSEFEVKNMELRPGDKLKIKGKILEDAERFQIDLGRGPEDLALHFNPRFHDGADGEVIVCNSKREGSWGSEQRDRTFPFQRGAEVKLTVKVTGQGFEVELPDGRELFFPDHLGLDTLTYIRVRGHFKVTSFKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5N141</accession>
    <name>W5N141_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gasdermin pore forming domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N141"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014350"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014379.1">
        <property type="protein sequence ID" value="ENSLOCP00000014350.1"/>
        <property type="gene ID" value="ENSLOCG00000011677.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155880"/>
    <dbReference type="HOGENOM" id="CLU_2025947_0_0_1"/>
    <dbReference type="InParanoid" id="W5N141"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011677">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF3">
        <property type="entry name" value="DEAFNESS, AUTOSOMAL DOMINANT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="122" mass="13344" checksum="2C7104C43E5E642C" modified="2014-04-16" version="1">MEHSLVQQSRGRRGEVFCLLKEKVFTTQTCSIAEQVQEQESCGGRLSFAPRRIQVSVNENGSLQLDSNVILEIPPKTTLAYSIIELDVKLNGEYGEQAVVRRSLPSGAVQGGEGRSPAQGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2W4</accession>
    <name>W5N2W4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NBR1 autophagy cargo receptor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2W4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014973"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015002.1">
        <property type="protein sequence ID" value="ENSLOCP00000014973.1"/>
        <property type="gene ID" value="ENSLOCG00000012171.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4582">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016335"/>
    <dbReference type="HOGENOM" id="CLU_014175_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2W4"/>
    <dbReference type="OMA" id="CEERTIQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012171">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14319">
        <property type="entry name" value="UBA_NBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000033">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000072">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000199">
        <property type="entry name" value="next to BRCA1 gene 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056893">
        <property type="entry name" value="UBA_Nbr1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF2">
        <property type="entry name" value="NEXT TO BRCA1 GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24932">
        <property type="entry name" value="UBA_NBR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="935"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="712"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="772"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="862"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="636"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="732"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="775"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="879"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="988" mass="108245" checksum="59AE4083D037D7D3" modified="2014-04-16" version="1">AMDSPVSLKAHFKGRAESLLVSDSERTRWEAVEVAVKHSFRLSDVQIKYLDEDNEEVSINSQEEYEEALKSAKKQGNRLRVNVYEVKGSSGTGERERGGGKRDHDVITTGVKPMMHCSAPAKPVSQNVPAASPVKETLVIKEVKVNRENDQAPPAWFTSYMEKFKDQLVKEAVERICQDFSERFCIQKVSQPSSEEPGSVAAQVQEVSSTAADRPKLPACQSCQAQTQRGSYLCSVCPSYTLCEQCSYGHDPTHSLVKIRTLVPPLFDCGTSVTGESGRLQKRIDKSFRKAEKQRLKAEKRQLKAEVREIKKQLRMERRSAQWSAAGEGAADPVLLQPRGTVASGAQSPKQSCAPMVPTMTAVFLDENLPDGTRLQPGTKFIKYWRMKNTGTVSWTVETKLKFMWGNLDLATGEKKEVVVPFLQPGQVGVVCVEFVAPGQEGTYTSHWRLAHRGEQFGPRVWCSIVVDPLTAEYCAEGQDPALCSKPEDIFVHMILNSEMCPCVALITNMLFSMLIQYNEVFFGLHTSSFMLCASFPRLEGVVRSRWRVVRLCVSAADMTPCMSPLPCDGPLLEKPGLGLIEEEAEGSALKPLLGVPLGEQPQAGSGPAQEEGEEDISGTQFVCETVIRSLTLEEAPDHTPPRRNKLDSQRSKSCVQRGGKGFMSSSLLVDSLQGMITVECVVLEESRSSLHKKASDVQVCKAQLPVQLLPPEECEESDIESVHAGSGSEREGDEEMAQDDQDEVQSQGSSASSEDYIIILPDCFDTSRPLGESMYSSALSQPGTEASSMETDSAPEQSELGAPEGQRAAAVPGHSVNDMLCASQTLDAVPLTPEVVPTPATLQPRSWRECLTTHQLWDAVRVRDGDSLSDPGGGSRPHPPEEEQARQSEESDSHARSQGGIAGGLVKGALLVAASAYKALFTGQPVPAQPPADSASQQAAMMEVLLEMGFGDRQLNQRLLKKHNYNLLDVVNELVQMTDNDWYSTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2X2</accession>
    <name>W5N2X2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Delta-1-pyrroline-5-carboxylate synthase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="20">Glutamate 5-kinase</fullName>
                <shortName evidence="20">GK</shortName>
                <ecNumber evidence="20">2.7.2.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Gamma-glutamyl kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Gamma-glutamyl phosphate reductase</fullName>
                <shortName evidence="20">GPR</shortName>
                <ecNumber evidence="20">1.2.1.41</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Glutamate-5-semialdehyde dehydrogenase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="20">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="23" type="primary">ALDH18A1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Can form homodimers/multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">In the N-terminal section; belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="20"/>
    <dbReference type="EC" id="1.2.1.41" evidence="20"/>
    <dbReference type="EMBL" id="AHAT01017297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2X2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014981"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015010.1">
        <property type="protein sequence ID" value="ENSLOCP00000014981.1"/>
        <property type="gene ID" value="ENSLOCG00000012172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044903"/>
    <dbReference type="InParanoid" id="W5N2X2"/>
    <dbReference type="OMA" id="PPMFIVD"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012172">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04256">
        <property type="entry name" value="AAK_P5CS_ProBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000010">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000011">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041744">
        <property type="entry name" value="G5K_ProBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005766">
        <property type="entry name" value="P5_carboxy_syn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01092">
        <property type="entry name" value="P5CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036429">
        <property type="entry name" value="P5C_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 20" id="KW-0521">NADP</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 20" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="22">
        <location>
            <begin position="56"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="21">
        <location>
            <begin position="348"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR036429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="782" mass="85107" checksum="7E4EFC6A9ED23ADF" modified="2014-04-16" version="1">IMLRRLALCSRFKVATGSALSRISPHFTRAASQAKFPLTRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMSVPVLEARACAAAGQSGLMALYEAMFTQYSTCTAQILVTNLDFHDDQKRRNLNSTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMKADLLIALSDVEGLYDSPPGSDDAKLIDIFYPGDQQSITYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTHPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARHGGRVLAALLPEQRSEIICHLADLLTEKKDDILAANKKDMEVAVSGGKLSPPMLKRLSLSTAKLNSLAIGLRQIAVSSRDSVGQVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVAALAIASGNALLLKGGKEASNTNQTLHQLTQEALSIHGVKDAVQLVSTHEEVEDLCRLDKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGICHVYIDSEASVDKAIKIIRDSKCDYPAACNAMETLLVHRDLLRTPVFDQIIDMLRVERVKIHAGPRFASYLTFSPSEVKSLRTEYGDLECCIEVVDSMQEAIDHIHKYGSSHTDVIVTENEETAEQFLQQLDSACVFWNASTRFSDGYRFGLGAEVGISTARIHARGPVGLEGLLTTKWVLKGDGHTVADFSEQGSMKYIHENIPVIQRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N360</accession>
    <name>W5N360_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat and fibronectin type III domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015069"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015098.1">
        <property type="protein sequence ID" value="ENSLOCP00000015069.1"/>
        <property type="gene ID" value="ENSLOCG00000012248.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159856"/>
    <dbReference type="HOGENOM" id="CLU_006446_0_0_1"/>
    <dbReference type="InParanoid" id="W5N360"/>
    <dbReference type="OMA" id="RSPNMLC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012248">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061172">
        <property type="term" value="P:regulation of establishment of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039269">
        <property type="entry name" value="ANKFN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437">
        <property type="entry name" value="WIDE AWAKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="415"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="998"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1292" mass="145631" checksum="07C00AAFA93B90B8" modified="2014-04-16" version="1">MPSGETTSLLTQSVAADLELRARPLDRKAPVLGKTQSLDNTPVPVSPYSGQEGTALRKRRRLPCSPGGLLWNTAGAQQRRYDRDETLPASAEKHNSNSDKNDPVFIWGISHRPVLRKSISVDDRLFQQPHKEQHKLLSRLERSKNKLRSIHSLGSPSRCETQRQTENKIARFAQRLSQKPRDQSQSDALNKDIRHLALTGSPGTSQSLDRNFSVSMTQQMQNLHLSHAKKSSGPSSPSAAKRLYRNLSEKLKGSHSSFDEAYFFGRSDRFRKTSVNVQCSEALFEAVEQQDLDAVQILLYQYTADELDLNTPNSEGLTPLDIAIMTNNVPMAKLLLKAGAKESPHFVSLESRALHLTTVVQEAQQRVSELSAQVLSEGLNLDSSEKEKQLKAWEWRYKLYKRIKTGFEHARAPETPSSVRLSVTSSTSLTVTFQEPLSINSAVVTKYKAVEWSYLKDFSSLAGELIMDNLQTLRCTITGLTTGKAYYVQVSAYNMKGWGPAQLATPPFAVPSNWKECDGREPRHRGQIEAMERLLQQVRATHHHYSCGDTSKLQNSSRKQSVSRSLKHLFHSSNKFVKTLKRGLYVAAVFYHKDSLLVTNEDQIPIVEIDDSYTSSIMQDFLWFTKLSCMWEDVRWLRQSMSVSMSSSSTLQARQKMLSAAGQLQNLLGTHNLGRVYYEPIKDRHGNVLIVTIREMESLYSFFNGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYQRRSQHRLSPGLYLGYLKLCSSVDQIKVLVPQRLPNVLCHFKIRDNCNVSRDEWEWLQKLSGPAYVESADQTSVCHTPPFFYELQMAVKGLLRQINLPLHQAKLFRIYTQEVLELGHNVSILLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELVHFCTYKEKFISLYCRLSAVLDLDSLITQQSLREAISDTEVATAKQRHQHVLDYIQQMDEMWREMRWIMDALQYARYKQPVGGVPITWFVDTTVEHSVEKNDSTSSNMDYLPTPSPSPEMRRRKAVSDSQPGSDEDGCSEVFLPTDSDYDSSDALSPRELDLVYSSSQDISQQAVCGLSGSAPDVLQVHDLKYSFCLKEDTGVSSGFDTEGEFCSKNMEDLTLSGLTSKGADKGSRGKQFGSKKKLLSKQQHHSYFSGHHRWLRVHSESQAVSLSEGVYTQQRETDLPLESTTGGAMNIFTELPRSSFLPHASSLPEDGKNRHRESKQNVRRIFVEPCKKTSLSEDTHTWTLNVGESEAATGVVVGFECAVAQDVDSDDQTNSQVSEIFSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3V6</accession>
    <name>W5N3V6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arylsulfatase G</fullName>
            <ecNumber evidence="11">3.1.6.1</ecNumber>
            <ecNumber evidence="14">3.1.6.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">N-sulfoglucosamine-3-sulfatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin.</text>
            <dbReference type="EC" id="3.1.6.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an aryl sulfate + H2O = a phenol + sulfate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140317"/>
            <dbReference type="EC" id="3.1.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="11"/>
    <dbReference type="EC" id="3.1.6.15" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01000343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3V6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015315"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015344.1">
        <property type="protein sequence ID" value="ENSLOCP00000015315.1"/>
        <property type="gene ID" value="ENSLOCG00000012439.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685202"/>
    <dbReference type="KEGG" id="loc:102685202"/>
    <dbReference type="eggNOG" id="KOG3867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159093"/>
    <dbReference type="HOGENOM" id="CLU_006332_13_6_1"/>
    <dbReference type="InParanoid" id="W5N3V6"/>
    <dbReference type="OMA" id="HVACRCQ"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012439">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033889">
        <property type="term" value="F:N-sulfoglucosamine-3-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.10:FF:000006">
        <property type="entry name" value="Arylsulfatase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000031">
        <property type="entry name" value="arylsulfatase G isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="17">
        <location>
            <begin position="39"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="529" mass="57788" checksum="8D3F65E76F2E6359" modified="2014-04-16" version="1">MPAVSVPLLLSGAVFTALVYFTTNHLGRRDTYSYLKKKPNFIIVLADDIGWGDLGLNRPGMNHTPNLDRMAQEGMRFTDFHSPASTCSPSRAALLTGRHGLRNGVTHNFAVGSVGGLPLNETVLAEVLQAAGYYTAMIGKWHLGHRGPYHPSHRGFDYYFGIPYSNDMGCTDTPGYDIPSCPPCARDPLLDGDEHKECYSRIALPLFENLRIVEQPLNLWTLAEQYTQKAIDIIHHARASGQSFFLYVALAHMHVPLSAASREPSITHIDPYAASLEEMDSFVGRIKTASDASDKENTLIWFTGDNGPWEKKCQFSGSVGPFLGRWQTDRGGSSAKRTTWEGGHRVPTIAYWPGKIPANVTSSALLSGLDIFPTVLSLAGESLPSQRQYDGIDVTKVLLHQSDTGHKSLFHPNSGAAGHFGDLQTVRLGQYKAFYITGAAEACGGGTGQEMVHDPPLIFDLSRDEGEGVPLDSSSAEYSMVLERVEKERQAVLTDIAMDNVSRADYAIDPSAAPCCDRSHVACRCRGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3W1</accession>
    <name>W5N3W1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein HB9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <sequence length="314" mass="35021" checksum="EC2A531CB3F64AC2" modified="2014-04-16" version="1">MEKSKNFRIDALLAVDPPKVQTSPLALVTSLSSSSISSSGSVTASELNNSTDSLRTETPSPPRMSSCGLIPKPGFLNATHSGMVGLHPQSSSGIPAQALYGHPMYTYSAAALGQHPALSYSYPHGSHHHHSSEPIKLTAGTFQLDHWLRVSTAGMMLPKMPDFNTQAQSNLLGKCRRPRTAFTSQQLLELEHQFKLNKYLSRPKRFEVATSLMLTETQVKIWFQNRRMKWKRSKKAKEQAAQDAEKQKGGKNNQDKSDGYQEQTDYQKGDSVKSSRMRDFRDSDDDEGEGFLYNSSDCSSEDERNHNRQISPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N690</accession>
    <name>W5N690_LEPOC</name>
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        <recommendedName>
            <fullName evidence="17">Probable proton-coupled zinc antiporter SLC30A4</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zinc transporter 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Probable proton-coupled zinc ion antiporter mediating zinc import from cytoplasm potentially into the endocytic compartment. Controls zinc deposition in milk.</text>
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    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
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        <property type="component" value="Linkage group LG3"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Cation_efflux"/>
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        <property type="entry name" value="Cation_efflux_CTD"/>
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        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
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        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
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        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF27">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="22">
        <location>
            <begin position="123"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="23">
        <location>
            <begin position="342"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="435" mass="47132" checksum="29C428E0E734E9E1" modified="2014-04-16" version="1">PPPVGTMSGAGVLQRLRRVFQGKPPEWDLSDTAAFDFSDELADEETPGFNKLRVVVSGEPGDFPAGASANGTAISAETDDDDSLLGSSSSLQSPGIAPDPCEGCTKKTHRLKQSRAMKKLGFAALLYLLFMIGELVGGYVSNSLAIMTDALHMITDLIGIVVSLLALWLSAKPPTKRFTFGLHRLEVISAAISVLLIYILTGALLYEAVQRTLHQDFDINGDVMLITAAVGVAVNVLMGFLLNQSGHLHSHSHSHSHSHGPSPAAHSSGQSHSSLAVRAAFIHALGDLLQSIGVLVAAYIVRFKPEYKIADPICTYIFSVLVLFTTVRIIWDAGIIVLEGVPRHLDVARIKEDLLKLEDVHSVEDLNVWALTTDKTAAVVHLQLLPSSATKWEEVQSKARLLLLNTYGVYRCTVQLQSFRQRPLKSCTKCQVARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N724</accession>
    <name>W5N724_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01030187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N724"/>
    <dbReference type="FunCoup" id="W5N724">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016433"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016463.1">
        <property type="protein sequence ID" value="ENSLOCP00000016433.1"/>
        <property type="gene ID" value="ENSLOCG00000013333.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158980"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="W5N724"/>
    <dbReference type="OMA" id="ESRFPEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013333">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="70"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="38949" checksum="92CC3D52E7EDA3F2" modified="2014-04-16" version="1">MSNKENQEPRSALRQPFGTVPGTMGPQRVLRNPVSEATSHNLIMGPGRVAVSSNQSSCLRPAKRFSIKDFDIGRPLGKGKFGNVYLARDRKLKFIVALKVLFKSQIEKEGVEHQLRREIEIQSHLRHPNILRFYNYFHDRTRVYLILEYAPQGEMYKQLQRCGRFNDQRTATYMEELADALQYCHERKVIHRDIKPENLLLGFRGELKIADFGWSVHAPSLRRRTMCGTLDYLPPEMIEGRTHDEKVDLWCIGVLCYECLVGNPPFETSSHTETYKRITKVDLHFPKTVSEGARDLISKLLRHSPTMRLSLKGVQEHPWVKSNSRRVLPPVFTPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7C7</accession>
    <name>W5N7C7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum membrane sensor NFE2L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Nuclear factor erythroid 2-related factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Nuclear factor, erythroid derived 2, like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">NFE2L1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7C7"/>
    <dbReference type="FunCoup" id="W5N7C7">
        <property type="interactions" value="954"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016536"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016566.1">
        <property type="protein sequence ID" value="ENSLOCP00000016536.1"/>
        <property type="gene ID" value="ENSLOCG00000013411.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="HOGENOM" id="CLU_024173_1_0_1"/>
    <dbReference type="InParanoid" id="W5N7C7"/>
    <dbReference type="OMA" id="KPWRDPF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013411">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF31">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE SENSOR NFE2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BZIP" evidence="26">
        <location>
            <begin position="702"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="517"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="629"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="805"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="811"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00020485"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022968"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="831" mass="90023" checksum="DC14FC4318F45FDF" modified="2025-10-08" version="2">MPHWGALDLPAGRRLQIHGCKEMLYLKKYFTEGLIQFTILLSLIGVRVDVDSYLSSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDDYFTARRLLSEVRALDRFLVPSTELSTWLVHRDPEGVGSAGQSSPGIALDNGGGLEDVGNPEGSAMRGEGPPPGSGYGLAGAEESLGAVAPQEGEEGEGGGRGEGDGGDDLTKEDRDQILILFSKTGPPVSDCSLINLSKRKLRETNGSKPSPCVQKLALQSRDSQRPLCGMVTGPQLGQSGRSLPAKLPGLGDQTTLSLQECLRLLEATFPFGDEAEFPGSVTPEVHEVGPSTSQGPLLSPLLPETDAPLDLEQQWQDIMAIMELQDMEVNSVTDSSYLNGNTSSSGNSSGMDTGSVESFGLSSPTPIHQDVSLHQASLPSCSQDFPAFFSPDLDSGCSLEAPGQPSALLSLSSSNSTDVNSTFGATNLTGIFLPPALNGTGNSTLEPAMPDPLGGLLEEAMLDEISLLDLAMEEGFSQAQASQLEEELDSDSGLSLDSSHSPVSPSSSETSSSSSSSSASVSFSEEGAVGYSTDSEAVADLEEGAVGGYQPEYSKFCRMSYQDPALFHQLPHLEHVSHNHTYNLPLSSHAEKPQAAGAGKKPSREKPQHQPPQDLLDKQSSRDERRARAMKIPFSNDKIINLPVEEFNELLAKHHLSEAQLALVRDIRRRGKNKMAAQNCRKRKLDTILNLEHGVEELRRDKTRLLKEKMEFVRSIRQMKQKVHSLYQEVFSSLRDEHGRPYPASDYALQYGSDGSVLLIPRSLAAEQARKQERKHKDRKKKTTQRSTSALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7J6</accession>
    <name>W5N7J6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Hepatocyte nuclear factor 4, gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7J6"/>
    <dbReference type="FunCoup" id="W5N7J6">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016605"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016635.1">
        <property type="protein sequence ID" value="ENSLOCP00000016605.1"/>
        <property type="gene ID" value="ENSLOCG00000013459.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4215">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158224"/>
    <dbReference type="InParanoid" id="W5N7J6"/>
    <dbReference type="OMA" id="WLTNETC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013459">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06960">
        <property type="entry name" value="NR_DBD_HNF4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06931">
        <property type="entry name" value="NR_LBD_HNF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000007">
        <property type="entry name" value="Hepatocyte nuclear factor 4 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000012">
        <property type="entry name" value="Hepatocyte nuclear factor 4, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049636">
        <property type="entry name" value="HNF4-like_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049635">
        <property type="entry name" value="HNF4_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="61"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="151"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="51742" checksum="EE28B831ACCAE509" modified="2014-04-16" version="1">RLLERMTLSESLLDMDVPNYSEGLDPDYTTLEFENTHVLYGDTDPVPVEPAAMDTPESGLSSLCSICGDRATGKHYGASSCDGCKGFFRRSIRKRHVYSCRFNRQCVIDKDKRNQCRSCRLNKCFHAGMKKEAVQNERDRISSRRNAYEAQSLPPITVLTQAESLSRQISLPSPGVPTDISAKKTASITDVCDSMKQQLLVLVEWAKYIPAFCELPLDDQVSLLRAHAGEHLILGVAKRSVPYKDILLLGNDHIIHRNCPEAEICRVANRILDELVTPFQEIQIDDNEYASLKAIVFFDPDARSLGDPSKIKSMRCQVQMSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMVEQLQFVKLFGLAKVDSLLQEMLLGGTAAEPTHFHHPVHPHVAQDPLTGQTVLISTLPTPVHPEQIATPETPLPSPPQGSGPDIYKVITDQAVGVPGQYVPITQSPDVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7U0</accession>
    <name>W5N7U0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymethylenebutenolidase homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dienelactone hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015210458.1">
        <property type="nucleotide sequence ID" value="XM_015354972.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7U0"/>
    <dbReference type="FunCoup" id="W5N7U0">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016699"/>
    <dbReference type="ESTHER" id="lepoc-w5n7u0">
        <property type="family name" value="CMBL"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000016729.1">
        <property type="protein sequence ID" value="ENSLOCP00000016699.1"/>
        <property type="gene ID" value="ENSLOCG00000013544.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698493"/>
    <dbReference type="CTD" id="134147"/>
    <dbReference type="eggNOG" id="KOG3043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000183"/>
    <dbReference type="HOGENOM" id="CLU_054590_8_2_1"/>
    <dbReference type="InParanoid" id="W5N7U0"/>
    <dbReference type="OMA" id="QCGAKHI"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013544">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000178">
        <property type="entry name" value="Carboxymethylenebutenolidase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042946">
        <property type="entry name" value="CMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46812">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46812:SF1">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="245" mass="27985" checksum="2DD4156E7EE32804" modified="2014-04-16" version="1">MANEARPCPCDLGDKLEYGGQGEEVQVEHLRAYVSKPGSSTDKAVIVIQDIYGWQLPNTRYMADMLASSGYIAICPDFFVGKEPWDPSKDWSTFQEWLQDRSATNINREADAVLGFLKERCGARRIAVVGFCWGGIATHHLILHYPELKAGVSVYGIIRDSEDRYSLRSPTLFLFAENDQVIPLDQVSTLEKKLQEHCTVDFKVKVFAGQTHGFVHRKREDINPNDRPFIEEARRDMLNWLNKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7X9</accession>
    <name>W5N7X9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033578">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033579">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7X9"/>
    <dbReference type="FunCoup" id="W5N7X9">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016738"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016768.1">
        <property type="protein sequence ID" value="ENSLOCP00000016738.1"/>
        <property type="gene ID" value="ENSLOCG00000013571.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3611">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158036"/>
    <dbReference type="HOGENOM" id="CLU_005410_1_0_1"/>
    <dbReference type="InParanoid" id="W5N7X9"/>
    <dbReference type="OMA" id="YHTRSTC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013571">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000048">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000021">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000003">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057563">
        <property type="entry name" value="Sema5A/B-like_TSP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF78">
        <property type="entry name" value="SEMAPHORIN-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23260">
        <property type="entry name" value="TSP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867365" description="Sema domain-containing protein" evidence="13">
        <location>
            <begin position="30"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="972"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="41"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1078" mass="120451" checksum="7B11716A11369546" modified="2014-04-16" version="1">MALATGGGPARHLLSLQGLLLLLCSRAAGWTQDPQCLRAEHPVIAFADIQPWIHTFRTEDTVDYSQLTFDPGQNELIVGARNHLFRLRLEDLSLIQGAEWQCDEFTKRACFSKGKSEDECQNYVRVLLVNGGWLFTCGTNAFTPICTNRTLTNLTEVHDQISGMARCPHNPLHNSTALITSSGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTIFSRAARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEIPFYYNELQGTFLLPELDLIYGIFTTNVNSIAASAVCVFNLSAVTQAFSGPFKYQENSRSAWLPYPNPNPDFQCGTIDFGSNGNLTERNLQDAQKFILMHEVVQPAAPVPYFMEDNVRFSHIVVDVVQGKDMLFHIVYLATDYGTIKKVLSPMNQSSSSCLLDEIELFPPSKRQPIRSLLILHSQSVLFVGLRDRVVKIPLMRCSIHRTQEGCVGARDPYCGWDLVLKRCTALEESLSMSQWEQSITECPTKNVTVDGGFGPWSPWKPCSHSDGGSVGPCLCRARSCDSPSPQCGGRACEGSSVEVTNCSSTRNGGWTPWSAWSHCSTSCGIGFQVRQRSCSNPAPRHGGRVCVGQNREERYCNEHLPCPPHVYWSSWGPWERCTAPCGGGVQARRRTCEKGKDCPGCSLEYQSCNTLSCPEMKKTTPWTPWAPVNISDNGGHYEQRFRYTCKARVPELSLLEVGRQRIEMRYCSSDGSTGCSTDGDLLRSGRFTAHTVNGGWSSWTPWSQCSRDCSRGIRSRKRVCNSPEPRYGGMPCLGPSLEYQECNITPCPVDGSWSCWSPWSKCSVTCGGGHYMRTRTCTSPPPAFGGDICLGLHTEEALCNTQPCPESWSEWSRWSDCDSSGTQLRVRRCDVLFPTGKQCWGNNTEARACLSDSNLIPEISIARSSREERRCGDFNLFHMIAVGLSSSILGCLITLLVYTYCQRYQQQSHDATVVHPISAAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFQPHLAGKTYTNAYFTDLNTYDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8L7</accession>
    <name>W5N8L7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hairy and enhancer of split-related protein HELT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HES/HEY-like transcription factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630162.1">
        <property type="nucleotide sequence ID" value="XM_006630099.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8L7"/>
    <dbReference type="FunCoup" id="W5N8L7">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016976"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017006.1">
        <property type="protein sequence ID" value="ENSLOCP00000016976.1"/>
        <property type="gene ID" value="ENSLOCG00000013768.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691597"/>
    <dbReference type="KEGG" id="loc:102691597"/>
    <dbReference type="CTD" id="391723"/>
    <dbReference type="eggNOG" id="KOG4304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160388"/>
    <dbReference type="HOGENOM" id="CLU_084227_0_0_1"/>
    <dbReference type="InParanoid" id="W5N8L7"/>
    <dbReference type="OMA" id="GHTHANA"/>
    <dbReference type="OrthoDB" id="6371181at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013768">
        <property type="expression patterns" value="Expressed in embryo and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11408">
        <property type="entry name" value="bHLH-O_HELT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000054">
        <property type="entry name" value="hairy and enhancer of split-related protein HELT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="11">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="235" mass="26497" checksum="DEB7820B52E1C2B6" modified="2014-04-16" version="1">MTSRMKERKKTPVSHKVIEKRRRDRINRCLNELGKTVPMALAKQNSGKLEKAEILEMTVQYLRALHSADFPRGREKGELLAEFANYFHYGYHECMKNLVHYLTTVERMETKDTKYARILAFLQSKSRVVTEPVFGSIGTLPDASDYLCQLHSTVDYQSHSPADSVFPPAPGHFSWHSSARSPSLPYPSVPISASPPQHGGYLSPVQGLDHHHYLNFIGHPHANAFSLHGAQHAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5N992</accession>
    <name>W5N992_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 17">Peroxisome proliferator-activated receptor gamma</fullName>
            <shortName evidence="17">PPAR-gamma</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Nuclear receptor subfamily 1 group C member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PPARG</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. May play a role in the regulation of circadian rhythm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer with other nuclear receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N992"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017201"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017232.1">
        <property type="protein sequence ID" value="ENSLOCP00000017201.1"/>
        <property type="gene ID" value="ENSLOCG00000013954.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158273"/>
    <dbReference type="HOGENOM" id="CLU_007368_4_2_1"/>
    <dbReference type="InParanoid" id="W5N992"/>
    <dbReference type="OMA" id="EMPFWPL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013954">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030522">
        <property type="term" value="P:intracellular receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010887">
        <property type="term" value="P:negative regulation of cholesterol storage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045923">
        <property type="term" value="P:positive regulation of fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06965">
        <property type="entry name" value="NR_DBD_Ppar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06932">
        <property type="entry name" value="NR_LBD_PPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000017">
        <property type="entry name" value="Peroxisome proliferator-activated receptor gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000010">
        <property type="entry name" value="Peroxisome proliferator-activated receptor gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003074">
        <property type="entry name" value="1Cnucl_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003077">
        <property type="entry name" value="PPAR-gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022590">
        <property type="entry name" value="PPARgamma_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF488">
        <property type="entry name" value="PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12577">
        <property type="entry name" value="PPARgamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01288">
        <property type="entry name" value="PROXISOMEPAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01291">
        <property type="entry name" value="PROXISOMPAGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0010">Activator</keyword>
    <keyword evidence="9 17" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="18">
        <location>
            <begin position="106"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="19">
        <location>
            <begin position="249"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="484" mass="55548" checksum="97137CBDFEC48318" modified="2014-04-16" version="1">MVDTQMPVWPMNFGLNAVDLADLDDSSQSFDMKPFTTVDYSSISSLAYETSPLPSEDLAQFDYMYDFKMNENPNTIKMEPHSPPQYSEKGQPYSKLLEDNANSMLNIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLVYDRCDLNCRIHKKSRNKCQYCRFQKCLMVGMSHNAIRFGRMPQAEKEKLLAEFSSDTDHMNPESADLRALAKHLYDSYLKSFPLTKAKARAILSGKTGDIAPFVIHDMKSLTAGEQLIHCKQVPLLEHRQEQGFSHEPNDEAELRFFQRCQVRSVEAVREVTEFAKSIPGFINLDLNDQVTLLKYGVHEVLISMWASLMNKDGTLISYGQIFMTREFLKSLRKPFCEMMEPKFDFSVKFNALELDDSDMALFLAVIILSGDRPGLVNVKPIEDLQDTVLQALELQLKLNHPESLQLFAKLLQKMTDLRQIVTDHVRLMQLLKKTEVDMCLHPLMQEIIKDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9A6</accession>
    <name>W5N9A6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9A6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017215"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017246.1">
        <property type="protein sequence ID" value="ENSLOCP00000017215.1"/>
        <property type="gene ID" value="ENSLOCG00000013966.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183074"/>
    <dbReference type="InParanoid" id="W5N9A6"/>
    <dbReference type="OMA" id="TCMGDIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013966">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003543">
        <property type="entry name" value="SR-AI/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF24">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01408">
        <property type="entry name" value="MACSCAVRCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="374"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="425" mass="45464" checksum="1B2552CF74FE7C73" modified="2014-04-16" version="1">MMAKWNKCTDDTEQITFVDNSLDACSLNADSLKNKESEEMKKKLCSLRTAIIVLYVIVMCVMAGFIYIIAGTGKTKEALGTVSGNGVSKQTGGTDEINGDTQKGFTNVDEGSHSGSLIKLTQNVEALTEQVDFVKESLSRSSSALEGNISKLTEDVKQLTYTLDLKVNYTLSVIQTTESTLQDTANSINDLKDDINGIRGMVKFVNSTCMGDISIHSQNIDQITSALRNVTVTTKWLKEKHTNIATEVKEEMAILKNITEDLRLKDWEQSLALSNITAIQGPPGPRGEKGNKGEGGFPGIPGPPGVSGLPGQKGDKGERGIGIPGIPGPPGPKGSAGVIGLQDFQRLKGEKGEKGDPGVMAEPSSSPPAPAAVVRLVNGLTNNEGRVEVLHDGEWGTICDDKWDLLDGMVVCKMLGFRTSLKVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9R6</accession>
    <name>W5N9R6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat and death domain containing 1A</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9R6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017375"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017406.1">
        <property type="protein sequence ID" value="ENSLOCP00000017375.1"/>
        <property type="gene ID" value="ENSLOCG00000014104.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154170"/>
    <dbReference type="HOGENOM" id="CLU_000134_51_1_1"/>
    <dbReference type="InParanoid" id="W5N9R6"/>
    <dbReference type="OMA" id="RFYKWEQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014104">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052457">
        <property type="entry name" value="Ankyrin-DD_containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24125">
        <property type="entry name" value="ANKYRIN REPEAT AND DEATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24125:SF0">
        <property type="entry name" value="ANKYRIN REPEAT AND DEATH DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="413"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="500" mass="56453" checksum="16A942143E15DB6A" modified="2014-04-16" version="1">VLRSEKEFHDAAKRNDTDRMQELIRRGVDVKAKNNIDRKALHWAAGAGHEQALRLLLQHDAAVDDEDCFGMNALLLASWFGHLKILQILVNAGAKINCENKNGLNLLHCAAQRGHIKVMEFIMEDLEDVRVDKLDKSGRTAFHLAAEHGQLELVTTIISLPRVFFLRKKYFDEQNTALHLAAKNGYSKVLQKIVETGVDVDEKNIEGLTALHFSAEGGHYECVKLLLESGCDINAQTYKNMSALHYVALHGYENVVRLLLEGGINMDAVNNQHATPLHIAVLSNFPGTVKLLIDDKCDLDIADSRQQTALHIAAEHGRQDIAEMILIAGVNLKLLDKQGKTSLDVAARGNHVNLVDMIIKADRFYKWENDNLNSDSSSWVGKHLSFRQDHRLETQHIRSVIWRLATKYLKPGEWKKLAHYWKFTEAHIRAIEQQWTGTKSYKEHGHRMLLIWLHGVIVAGENPIKGLYEGLVGIARRDLAESIRRKANADTNSPKKCITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9X6</accession>
    <name>W5N9X6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein PALS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein associated with Lin-7 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632749.1">
        <property type="nucleotide sequence ID" value="XM_006632686.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206664.1">
        <property type="nucleotide sequence ID" value="XM_015351178.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206665.1">
        <property type="nucleotide sequence ID" value="XM_015351179.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206666.1">
        <property type="nucleotide sequence ID" value="XM_015351180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9X6"/>
    <dbReference type="FunCoup" id="W5N9X6">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017435"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017466.1">
        <property type="protein sequence ID" value="ENSLOCP00000017435.1"/>
        <property type="gene ID" value="ENSLOCG00000014151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688859"/>
    <dbReference type="KEGG" id="loc:102688859"/>
    <dbReference type="CTD" id="252845"/>
    <dbReference type="eggNOG" id="KOG0609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156087"/>
    <dbReference type="HOGENOM" id="CLU_001715_5_4_1"/>
    <dbReference type="InParanoid" id="W5N9X6"/>
    <dbReference type="OMA" id="FSHRTMT"/>
    <dbReference type="OrthoDB" id="43580at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014151">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016332">
        <property type="term" value="P:establishment or maintenance of polarity of embryonic epithelium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002011">
        <property type="term" value="P:morphogenesis of an epithelial sheet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000105">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015145">
        <property type="entry name" value="L27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035601">
        <property type="entry name" value="MPP5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09060">
        <property type="entry name" value="L27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="119"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="178"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="256"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="345"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="18">
        <location>
            <begin position="479"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="37"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="675" mass="76671" checksum="903B5A8D7600E8E8" modified="2014-04-16" version="1">MTTSHMNGHVTEDSDSDMKSGELLAHEEPQWHREMAVDCPGDLGSRAMPVRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLSQNPRIGIDNPTFEPIEGLPPQGPGSTSRILDLEDLLASLKHIQYSLSDSQSQEDISVVLQLIQKTDFQNAFQIHNAVALHMNKPSPPFPLTSNAQDLSQEVQAILRSSQQKDGLELNALLTSPHVQALMQAHDQVAEQELQPEPAGPQDGLYETLTQYGGETVKIVRIEKARDIPLGATVRNDMDSVVISRIVKGGAAEKSGLLHEGDEILEINGIEIRGKDVNEVFDILADMHGTLTFVLIPSQQSKPPPIKETVVHFKAHFDYDPSDDPYVPCRELGLCFQKGDILHVISQEDPNWWQAYRDGDEDNQPLAGLIPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKLLYNANKNEDYDNEEILTYEEMALYHQPANRKRPIALIGPPGCGQNELRQRLMANEPERFAAAVPHTTRSRRDNEVNSRDYHFVSRQAFEMDVGANKFIESGEFEKNLYGTSTDSVRQVINSGKICLLSLHTQSLKVLRSSDLKPYIIFIAPPNQERLRALLAKEGKNPKPEELRDIIEKAREMEQNYGHLFDAAIVNMDQDKAYQELLRLINKLDTEPQWVPSSWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAH8</accession>
    <name>W5NAH8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Si:ch211-107o10.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAH8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017637"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017669.1">
        <property type="protein sequence ID" value="ENSLOCP00000017637.1"/>
        <property type="gene ID" value="ENSLOCG00000014321.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165857"/>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_1"/>
    <dbReference type="InParanoid" id="W5NAH8"/>
    <dbReference type="OMA" id="NWDDLNW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014321">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF26">
        <property type="entry name" value="RETINOL DEHYDROGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="36173" checksum="7B5D1B749A2AC76F" modified="2014-04-16" version="1">LSGCAKGIRDFVQEHSSGITVAVVAARGVGLVVLRRWFAGGKCKSRARLDGKTILITGANTGIGKATAEELACRGARVILGCRDLTRANKAAEEIRQNSGNGNVVVKQLDLASLQSVRQLAKDILESEDRLDILINNAGIMMCPQWKTRDGFEMQLAVNHLGHFLLTNSLLDLLKKSSPSRIINVSSLAHEKGQINFDDINLEASYDPVTSYRQSKLANVLFTRELAARLKGTGVTANCLHPGVIRTELGRHLMPTIPVWKKVVFFPFLLLVFKTPRQGAQTTIHCAVDEDLEGVTGVYFADCAPKETAPQGQEDEAAKRLWDLSAQMVGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAR3</accession>
    <name>W5NAR3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAR3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017722"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017754.1">
        <property type="protein sequence ID" value="ENSLOCP00000017722.1"/>
        <property type="gene ID" value="ENSLOCG00000014394.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1376">
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    <dbReference type="HOGENOM" id="CLU_015718_0_1_1"/>
    <dbReference type="InParanoid" id="W5NAR3"/>
    <dbReference type="OMA" id="YMASCIL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014394">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:microtubule"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural constituent of cytoskeleton"/>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:mitotic cell cycle"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="alpha_tubulin"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
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        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
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        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
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        <property type="entry name" value="Tubulin"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
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        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="56"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="255"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="50667" checksum="2FB34ADCB85D2551" modified="2014-04-16" version="1">SLSAPLFQRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLSDQCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGVDSVEGEAEEGEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC69</accession>
    <name>W5NC69_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC69"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018260.1">
        <property type="protein sequence ID" value="ENSLOCP00000018228.1"/>
        <property type="gene ID" value="ENSLOCG00000014805.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5YU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097126"/>
    <dbReference type="HOGENOM" id="CLU_090787_0_0_1"/>
    <dbReference type="InParanoid" id="W5NC69"/>
    <dbReference type="OMA" id="VKCNKDK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014805">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14278">
        <property type="entry name" value="UBA_NAC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
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        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="23055" checksum="02E33ED9C78D29F0" modified="2014-04-16" version="1">VMCCWVCEKSLGTVRRCGRCKKVYYCSQECQLKHWPLHKELCWNSKMLTEDVDCFVSHSAETQLTMPAPLSEQDNIMGLNMLDHAVTVKVKHNKSKHTMVISEGTNGEECFSLISSRLQIPQEKMRLISKGKVIHKDNILNFLKENALFQAFGEQAECEDGLDQRDIEVLMSQLSVERNIAVRALHKTGDLIDAIFYISDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCI8</accession>
    <name>W5NCI8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Docking protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOK family. Type A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCI8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018347"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018379.1">
        <property type="protein sequence ID" value="ENSLOCP00000018347.1"/>
        <property type="gene ID" value="ENSLOCG00000014900.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687260"/>
    <dbReference type="KEGG" id="loc:102687260"/>
    <dbReference type="CTD" id="101882881"/>
    <dbReference type="eggNOG" id="KOG4047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159868"/>
    <dbReference type="HOGENOM" id="CLU_030101_2_0_1"/>
    <dbReference type="InParanoid" id="W5NCI8"/>
    <dbReference type="OMA" id="DWTQKLC"/>
    <dbReference type="OrthoDB" id="6020914at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014900">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01203">
        <property type="entry name" value="PTB_DOK1_DOK2_DOK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037751">
        <property type="entry name" value="Dok1/2/3_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF14">
        <property type="entry name" value="DOCKING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="5">
        <location>
            <begin position="161"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="494"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="551" mass="62101" checksum="31C5FF380AB70AAD" modified="2014-04-16" version="1">MEEAIRKQGVLYLQQQRFGKKWKKVWSTVYGESTCSIARMEFFECKDSGAHTEKPDKALRKQENKKVIKLSDCIRVSEVEMDGCPKDCVPFLVETTEKLFVFAAESAELEDWTQKLCETAFPMNWNERPSLSKRNSLQHSVRGDSSAAPMEENSLYNTRESVKEFKVTIRKTEATERCRLRGMYILRADFENLVLKDTKTGEALYTWPYRFLRRFGRDKVTFSFEAGRRCTSGEGNFEFETRQGNSLFQAIESAINVQKSTLAHHMKHFSSTEGDALPSPRSQAVGASGTYSMVSEVQGEVQQGSRQRMDLPSETLLSGVKSLTLDSRSTPRKPQVKTIASCPILSQEDQTYSQISMAPGEPLDHGKDRGPQKSQGKSSHRESEYAVPFDTIAKNLASTFRTFSIPTPEETGSERGRGKVPLEAPDPLYDSIDESAMNRAKIHKPAAYTRAEHIYDEPEGCAVAAATGTSLYDDPEEVKGYAWKLQAASMDPAGHEYPYNPSVDDYAVPKPPKKVLSTEESDEDVQDQEEENLDTTYDNVMLKSMEKMNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCV1</accession>
    <name>W5NCV1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Fibroblast growth factor receptor</fullName>
            <ecNumber evidence="26">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25 26">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="26"/>
    <dbReference type="EMBL" id="AHAT01006201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCV1"/>
    <dbReference type="FunCoup" id="W5NCV1">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018460"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018492.1">
        <property type="protein sequence ID" value="ENSLOCP00000018460.1"/>
        <property type="gene ID" value="ENSLOCG00000014985.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155860"/>
    <dbReference type="InParanoid" id="W5NCV1"/>
    <dbReference type="OMA" id="WIFVPCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014985">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05857">
        <property type="entry name" value="IgI_2_FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05098">
        <property type="entry name" value="PTKc_FGFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000007">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000020">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000252">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000011">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028174">
        <property type="entry name" value="FGF_rcpt_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016248">
        <property type="entry name" value="FGF_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF131">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000628">
        <property type="entry name" value="FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26 28" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19 29" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="23" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13 26" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 26 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 26 30" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8 26" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18 26" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="246"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="348"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="33">
        <location>
            <begin position="569"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="872"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="31">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="872"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="27">
        <location>
            <position position="714"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="575"/>
            <end position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28 30">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="653"/>
            <end position="655"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="28">
        <location>
            <position position="718"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="28">
        <location>
            <position position="732"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="149"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="271"/>
            <end position="323"/>
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    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="370"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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            <dbReference type="ARBA" id="ARBA00004514"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000628"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-1"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000628-2"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000628-3"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="913" mass="101777" checksum="AC2B8A7BC39C31BE" modified="2014-04-16" version="1">MPVAAGACPCAPSCWTVEHAPVLRPGAGPPLCTAPPTAATWAWSACCWPGGRTLFSVTTKAPPPCTRLAISRWQSGRPSADDPWLPCCALLTSRRGLRRMLPWTLVFLWAVLAQVSFSEGRTTITDQVPPETQAEPYTLYPGERLELRCGAKEEGQALVWTKDHVTLQDGEHTRLREALLEIEGVETADSGLYACVSQGPFSNHSIYFSVNVTADTLASSEDEDDDESSSEETKLSSSQKLLPMAPQWAQPEKMEKKLHAVPASKTVKFRCQASGNPVPTLKWYKNGKEFKREQRIGGFKVREHMWTIIMESVVPSDKGNYTCVVENKYGSINHTYQLDVVERSPHRPILQAGLPANRTVVVGSDVEFVCKVFSDPQPHIQWLKHIEVNGSRVGPDGLPYVRVLKTAGLNTTDKEMEVLQLKNVSFDDAGEYTCLAGNSIGFSHHTAWLTVLKAMPPSPVPSQTYLEVLIYCVGFFLICIMVVIAIIFKMKSTAKKSDFNSQLAVHKLAKSIPLRRQVTVSVDSSSSFHSGVMLVRPSRLSSSGTPMLSGVSEYELPQDPRWELSRDRLVLGKPLGEGCFGQVVMGEALGLDKEKPNRVTKVAVKMLKPDATEKDLSDLISEMEMMKIIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYCYNPDQVPVEHMSIKDLVSCAYQVARGMEYLSSKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSTCTHELYMMMRDCWHAVPSQRPTFKQLVEDLDRTLAMTSNQEYLDLSVPLDQYSPSYPDTRSSTCSSGEDSVFSHEGGAEEPCLPKFPPHPNGVAFKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND75</accession>
    <name>W5ND75_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alpha-mannosidase</fullName>
            <ecNumber evidence="17">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:56052"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28729"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="3.2.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01004844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND75"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018584"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018616.1">
        <property type="protein sequence ID" value="ENSLOCP00000018584.1"/>
        <property type="gene ID" value="ENSLOCG00000015097.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691193"/>
    <dbReference type="CTD" id="4122"/>
    <dbReference type="eggNOG" id="KOG1958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234638"/>
    <dbReference type="InParanoid" id="W5ND75"/>
    <dbReference type="OMA" id="CPWGQHP"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015097">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004572">
        <property type="term" value="F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000009">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF57">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="19">
        <location>
            <begin position="503"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1177" mass="134291" checksum="4AA6DE5FBFDA290D" modified="2014-04-16" version="1">MKLKKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQGVGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVLELTETGAGAPGGQIPFRSHNGSWVVPFDGRPAFLSVSPQDCQFALGNQGQKTDLQTLDVYSLLSFDNVDGGVWKQGFEITYDPSEWDNEPLQVFVVPHSHNDPGWIKTFDKYYFDQTQHIINNMLVKLQEDPRRKFIWSEISFFAKWWESVDMQKREAMSKLIRSGQVEMVTGGWVMTDEANVHYFAMIDQLIEGHQWLEKNIGVTPRSGWAVDPFGHSATMSYVLKRANLTSMLIQRVHYSIKKHFSATQSLEFMWRQAWDPASSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRVNCPWKVPPRAIVEANVAERAKLLLDQYRKKSKLFRSKVVLVPLGDDFRYDKAQEWDQQYINYQKLFDYMNSHPEMHVHAQFGTLTDYFDAVYTTNGVAHGTRPPDYPVLSGDFFAYADREDHYWSGFYTSRPFYKNLDRVLESHLRGAEILFSLALAHSRHAGMEGRYPTSDYSLLTDARRNIGLFQHHDAITGTAKEVVVIDYGVRLLRSLVGLKRVIINAAHFLILRNKDQYRFYQTEPFLETDDRRATQDSLPQRTLIELDSTPRYLVVFNPVEQERLCLVTVLVNSARVRVLTEDGQTLPVQLSAQWTSPSEMSQDGFQASFATRLPALGLAVFQLYDSFDSALTLRSDTVLRLNGRGLTPRGVDPLPLRVHASDQHDFYIQSQNLLLSFSGSTGLLESVTRKEDQQEVKLQMEFWIYGTRASKDKSGAYLFLPDGEPKPYMQKEPPIVRVVEGPLFCEVVTYYQHFQLTVRIHNLPGVDGLSLDITTLVDIRDQTNKELAMRLVTNIQSESFFFTDLNGFQIQPRRYFKKLPLQANFYPMPTMAYIQDSQYRLTLHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKRTENKFRILLERRVSASKHAGFLSTVSSMFHSLISHIFGGSTQEVQDSQPVSFPSLLSHMTSVYLNYGFLAFPVLPKKRGVPPLRSFAPLSGTMPCDFHLLNLRNIQNQGEQTPSLDTALILHRKGFDCSLEAWNLGFNCTTTQGKLSLSGLFQALDVQSVQPMSLSLMYAGTPLSNGSLIKMDPMEISTFKIRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDK0</accession>
    <name>W5NDK0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDK0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018709"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018741.1">
        <property type="protein sequence ID" value="ENSLOCP00000018709.1"/>
        <property type="gene ID" value="ENSLOCG00000015202.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102692141"/>
    <dbReference type="eggNOG" id="KOG2514">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158600"/>
    <dbReference type="HOGENOM" id="CLU_006685_1_3_1"/>
    <dbReference type="InParanoid" id="W5NDK0"/>
    <dbReference type="OMA" id="SQAFHPL"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015202">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF28">
        <property type="entry name" value="ANOCTAMIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="685"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="735"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="12">
        <location>
            <begin position="79"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="11">
        <location>
            <begin position="307"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="887"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="923" mass="107808" checksum="8056B30164ADF776" modified="2014-04-16" version="1">MEESSSGSPEPAVTNGKTPHPAIKDVNILLDESEGVNSPSKDDDSLLHPGTLTSTSEEASRGEGAAEGVKEKRKSSGLFFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLTNSDIIFLKLHAPWEVLCRYAELMNIRMPFRRKIYYLHRRYKFMSRMEKRISRFRGWLPRKPMKFDKDTLPDLEENDSYTAPFSQQRIHHFIINNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNSSYEAAFPLHEGSYRSKNSIRTHGAENHRHLLYECWAWWGVWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLFPTAVVGLLVFLYGVFTLDSCQVSKEVCQATDIIMCPLCDKYCPYMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVLAYDWDLIDWAEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVSASGIFFMILVVIAAVFGIVIYRVVTVSTFAAFDWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINRWKLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQEHGHKAKASFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFPLAPVLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGQAGEKCMVGYVNATLSIFQVSDFENRSEPRTDGTDMFGSPIKYCRYRDYREPPNSKQPYTYTLQFWHVLAARLAFIIVFEHLVFTIKNLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERNERKKNGKTHNNEWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG81</accession>
    <name>W5NG81_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adenosylhomocysteinase like 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG81"/>
    <dbReference type="FunCoup" id="W5NG81">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019640"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019672.1">
        <property type="protein sequence ID" value="ENSLOCP00000019640.1"/>
        <property type="gene ID" value="ENSLOCG00000015950.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182981"/>
    <dbReference type="InParanoid" id="W5NG81"/>
    <dbReference type="OMA" id="IVITCTX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015950">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:S-adenosylmethionine cycle"/>
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        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF2">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="7">
        <location>
            <begin position="380"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="68458" checksum="0A93F823CF7F618D" modified="2014-04-16" version="1">LRIRHPSPHVSVCVCVSLERGLCEIAAMSVQVVAAKMAEVELKDIPNKDLPPVPQVAPKAEEKPAVKNEPNAAAAASEATASGDPSPPTPSPNPMKMPQASAMKRTDPQQNGGEALVNRDGTVVEAPRTVKKIQFADQKQEFNKRPTKIGRRSLSRSISQSSTDSYSSAASYTDSSDDETSPRDKQQKNSKGSSDFCIKNIKQADFGRREIEIAEQEMPALMALRKRAQGEKPLAGAKIVGCTHITAQTAVLMETLSALGAQCRWAACNIYSTQNEVAAALAEGGFAVFAWKGESEDDFWWCIDRCVNVEGWQPNMILDDGGDLTHWIYKKYPNMFKKIKGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCAALKAMGSIVYVTEIDPICALQACMDGFRLVKLNEVIRQVDIVITCTGNKNVVVREYLDRMKNGCIVCNMGHSNTEIDVASLRTPELTWERVRSQVDHVIWPDGKRIVLLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDEYVASLHLPTFDAHLTELTDEQAKYLGLNKNGPFKPNYYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG99</accession>
    <name>W5NG99_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein 395b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008417">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008418">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG99"/>
    <dbReference type="FunCoup" id="W5NG99">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019658"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019690.1">
        <property type="protein sequence ID" value="ENSLOCP00000019658.1"/>
        <property type="gene ID" value="ENSLOCG00000015964.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW7P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157136"/>
    <dbReference type="HOGENOM" id="CLU_032989_2_0_1"/>
    <dbReference type="InParanoid" id="W5NG99"/>
    <dbReference type="OMA" id="WSSVVFW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015964">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031940">
        <property type="entry name" value="DUF4772"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF6">
        <property type="entry name" value="ZINC FINGER PROTEIN 395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15997">
        <property type="entry name" value="DUF4772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="334"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="440"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="498" mass="54616" checksum="B910607BBAB4FAFF" modified="2014-04-16" version="1">MATVVPKRLGKRSLLGTLVCAPCPSGDGLLVPGQIEAVHSEPGGAVRSCTVALEDGQLQEYSQDSIVGPGFQSLSRFKLYPGQKCFFFLTGSLGIIFEINHFVVLISISFALVSLSLQHCQKSLLNSSPYHNSLTFTSVSFLIQRPPPNSLTYKHQISNWIEHIVSRKHSVHMCFLMAAMVLTSLSCSPAVQSPPKKETVSGDCGIAVAGVTECGELSDSGSSGYWSWDLGNTSPTPSPPMAEDGPQTTPIDEGLDMELDHVLFDEPTPRKRKNSMKVAYKCLWPTCGKILTSVVGIKRHIRTQHLGQSSDLERSRREEDFYYTEIRREQEAALPCSPQLSCASPPRPTQQGPMGPGVCVSEQPTSGAQQPSPLSQSAPSAFWQVRSEHSYQANPPVRVTLSPVSPSTFSWTPSPPVLQSQQVASFRSRSVSVGEQWLQQHSVPPRTHAASSSPPRGHSSSRKTRGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH11</accession>
    <name>W5NH11_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA helicase B</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH11"/>
    <dbReference type="FunCoup" id="W5NH11">
        <property type="interactions" value="610"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019920"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019953.1">
        <property type="protein sequence ID" value="ENSLOCP00000019920.1"/>
        <property type="gene ID" value="ENSLOCG00000016165.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691212"/>
    <dbReference type="KEGG" id="loc:102691212"/>
    <dbReference type="CTD" id="92797"/>
    <dbReference type="eggNOG" id="ENOG502QWCN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006913"/>
    <dbReference type="InParanoid" id="W5NH11"/>
    <dbReference type="OMA" id="KIKHAWA"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016165">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17933">
        <property type="entry name" value="DEXSc_RecD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058839">
        <property type="entry name" value="WHD_HELB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF6">
        <property type="entry name" value="DNA HELICASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13604">
        <property type="entry name" value="AAA_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25894">
        <property type="entry name" value="WHD_HELB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase B winged helix" evidence="5">
        <location>
            <begin position="249"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="4">
        <location>
            <begin position="867"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="394"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="944"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1014"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="956"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1075"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1094"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1126" mass="125387" checksum="32DA0B0546D73063" modified="2014-04-16" version="1">MFYLGAPTGDRTLVGYILPRKAETAVKDEDESDSEEEDEDEPEFLDFKEMSSLSSGGLLVKGSYPRRREVDFQDTNLKKKCRVVGRFTLLSPWWEVTSPVRQRNSKVFLLGFPFYSLRTELGSDSRAIISLFLKACKVTPEHVTKFMDWLPDWMSLNFSNLRELLKDFGTEDCNGYKAIADSMLSSVNHSSAGRAVWAASMHPYVMKYLPVLLPRQFENLVAADEKPQKHAGTEDKNEAPDYRSLLGKLEEVIKTDVWKLGFSSVTYKELGLIRCEATWDAFWHSGLLPQIPDLQRCALMLYDKLKRLCRSRGSTFVYQKVLSDCPDVNQSVKALEALRFLTEQEVVVQEKQRVFLRQYYRYETGIAKCVGRVVAGTPWRIDLDVREALRAARPKGGEVGQDGRRAEAETESAADGAPSDVTADPARPEDLSDGREHRSRGAAEGQAAGEAELDPDQVRAAAMICANPVTVISGKGGCGKTTVVSLVFRAALKGLPSEEEEVRKACEDFENDCGASEEWNTFSEVPRQAQDKTGKEAVDSQEPIRVLLTAPTGRAASLLKKRTGLSAYTLHQVVWSFMQAKKAEDGAPKEWKFAKVQAFVVDEGSLVSVQILHSVLNMLVRYAQLKKLILLGDVRQLPSIEPGNVLADMFSSLSEVHWAVEMKTNHRAESQLIVNNAGQISDMGKKRSYYPLEFDATVELGGSGPSVTMPTLDKSFVFICLPTEANTCELQDAIKLLLKSAPGLTDHNSSQFIAFRRKDCDLINELCCKHYSGHNTRNHKGKLKFQPGDKVCCTKNGNVLERTRERDLNQEVDEKHKETVRLCNGEIFFITRDEEKDKKRYLTLVDGDCRELCVWFRELQRECRLKHAWARTIHTFQGSEAETIVYALSDGRGENWQHIYTAVTRGRRRVYVVGNKDALKNAVERSIPVRNTQLKEMVRKELNKRNQATSSTPHRAPSGPSSTPSTSLSANSQRLPQDQCSTTPLLRQNLLVRDSGTADVSLLDDIAFSQAYSWSPMDTQSPPVPNAENAGKGKTNGLDSGALPGPCDHQGSSGDSFRNSETDSRSPCGVKRQKSTSNGSHTPSKISREVAAESPLSSSRLQSLSLASPSSSSRARQLFPPPPPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH50</accession>
    <name>W5NH50_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">YEATS domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 diacetylated at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac) or histone H3 diacetylated at 'Lys-14' and 'Lys-27' (H3K14ac and H3K27ac). Also able to recognize and bind crotonylated histone H3. May also recognize and bind histone H3 succinylated at 'Lys-122' (H3K122succ); additional evidences are however required to confirm this result in vivo. Plays a key role in histone variant H2AZ1/H2A.Z deposition into specific chromatin regions: recognizes and binds H3K14ac and H3K27ac on the promoters of actively transcribed genes and recruits NuA4-related complex to deposit H2AZ1/H2A.Z. H2AZ1/H2A.Z deposition is required for maintenance of embryonic stem cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of numerous complexes with chromatin remodeling and histone acetyltransferase activity. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with MLLT10/AF10. Also interacts with the SWI/SNF component SMARCB1/BAF47, TACC1 and TACC2, and the nuclear matrix protein NUMA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633642.1">
        <property type="nucleotide sequence ID" value="XM_006633579.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH50"/>
    <dbReference type="FunCoup" id="W5NH50">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019959"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019992.1">
        <property type="protein sequence ID" value="ENSLOCP00000019959.1"/>
        <property type="gene ID" value="ENSLOCG00000016192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692619"/>
    <dbReference type="KEGG" id="loc:102692619"/>
    <dbReference type="CTD" id="8089"/>
    <dbReference type="eggNOG" id="KOG3149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155811"/>
    <dbReference type="HOGENOM" id="CLU_051385_3_0_1"/>
    <dbReference type="InParanoid" id="W5NH50"/>
    <dbReference type="OMA" id="VKPYHNE"/>
    <dbReference type="OrthoDB" id="16041at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016192">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16909">
        <property type="entry name" value="YEATS_GAS41_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1970:FF:000002">
        <property type="entry name" value="YEATS domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YEATS" evidence="14">
        <location>
            <begin position="15"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="226" mass="26418" checksum="C64DBC309A4E7E2F" modified="2014-04-16" version="1">MFKRMAEFGPDSGGRVKGVTIVKPIVYGNVARYFGKKREEDGHTHQWTVYVKPYRNEDMSAYVKKIQFKLHESYGNPLRVVTKPPYEITETGWGEFEIIIKIFFIDPNERPVTLYHLLKLFQSDSSAMPKKTVVSEFYDEMIFQDPTAMMQQLLTTSRQLTLGAYKHETEFAELEMKTKEKLEAAKKKTSVEITELKERLKASRETINFLKSEIRKLEEEDQVKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH75</accession>
    <name>W5NH75_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial fission regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in mitochondrial aerobic respiration. Regulates mitochondrial organization and fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MTFR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625949.1">
        <property type="nucleotide sequence ID" value="XM_006625886.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203517.1">
        <property type="nucleotide sequence ID" value="XM_015348031.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203522.1">
        <property type="nucleotide sequence ID" value="XM_015348036.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH75"/>
    <dbReference type="FunCoup" id="W5NH75">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019984"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020017.1">
        <property type="protein sequence ID" value="ENSLOCP00000019984.1"/>
        <property type="gene ID" value="ENSLOCG00000016211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690111"/>
    <dbReference type="KEGG" id="loc:102690111"/>
    <dbReference type="CTD" id="113115"/>
    <dbReference type="eggNOG" id="ENOG502QUU8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183215"/>
    <dbReference type="HOGENOM" id="CLU_059135_0_0_1"/>
    <dbReference type="InParanoid" id="W5NH75"/>
    <dbReference type="OMA" id="VCQKKEY"/>
    <dbReference type="OrthoDB" id="2133332at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016211">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007972">
        <property type="entry name" value="Mtfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215:SF2">
        <property type="entry name" value="MITOCHONDRIAL FISSION REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215">
        <property type="entry name" value="PROTEIN OF UNKNOWN FUNCTION DUF729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05308">
        <property type="entry name" value="Mito_fiss_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="368" mass="40381" checksum="6EF99EE36880A7C1" modified="2014-04-16" version="1">MSLFADLLDILRYILDYFGVPPDMLLPVWENQLCGQYRSIIRMIGTNLPLSPCPRVHFQIPLQACARPSTLNSSDENCAVPSFADVMWVAHDEGESYARLRNEVHPLDGKPVAFFTAPPTCLSVPSQHAQGVLEKKTTQPPTNSDALKKITALEDELLRLRAQIAMIVTTPPGPLCSEYESARAPGTPRMSRLPQVLTSTPVPAPPPAPPPLAPPLPPAPSCATPDLSVTDLIRQRRAASRTEQSHRERALPSSSSLPSMLDVLKDMNKVRLRTVERSPGGTPVMKKDKKIISSADPAALIAAALKRKFAHRYKEDSFDKENRPFETSPFGSPEIPRLPYQVTKGTGKLPPLKSDLLSLTPTIKANRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHP8</accession>
    <name>W5NHP8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="4">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01001684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638696.1">
        <property type="nucleotide sequence ID" value="XM_006638633.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHP8"/>
    <dbReference type="FunCoup" id="W5NHP8">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020157"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020190.1">
        <property type="protein sequence ID" value="ENSLOCP00000020157.1"/>
        <property type="gene ID" value="ENSLOCG00000016326.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693775"/>
    <dbReference type="KEGG" id="loc:102693775"/>
    <dbReference type="CTD" id="64263"/>
    <dbReference type="eggNOG" id="KOG2698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013481"/>
    <dbReference type="HOGENOM" id="CLU_049768_1_3_1"/>
    <dbReference type="InParanoid" id="W5NHP8"/>
    <dbReference type="OMA" id="ITSCMMD"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016326">
        <property type="expression patterns" value="Expressed in embryo and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050936">
        <property type="term" value="P:xanthophore differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF6">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="11">
        <location>
            <begin position="61"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="239" mass="26948" checksum="4486D814A7697E02" modified="2014-04-16" version="1">MELHKAATEMNGVCNGKIVTEFNCRNGFSELAIEAKKAAVQCKHDKSRKKCEDESNLQALEDAYTTILQGLGENTDRQGLLMTPRRAAKAMQFLTKGYHETIYEILNDAIFDEDHDEMVIVKDIDMFSLCEHHLVPFFGRVHIGYLPSKKVVGLSKLARIVEIYSRRLQVQERLTKQIAVAISEALKPAGVAVVIEAAHMCMVMRGVQKMNSRTVTSTMLGVFREDPKTREEFLSLIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIJ3</accession>
    <name>W5NIJ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">SIM bHLH transcription factor 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015205072.1">
        <property type="nucleotide sequence ID" value="XM_015349586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIJ3"/>
    <dbReference type="FunCoup" id="W5NIJ3">
        <property type="interactions" value="607"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000020452"/>
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        <property type="protein sequence ID" value="ENSLOCP00000020452.1"/>
        <property type="gene ID" value="ENSLOCG00000016561.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687809"/>
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    <dbReference type="eggNOG" id="KOG3559">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000156143"/>
    <dbReference type="HOGENOM" id="CLU_010044_4_0_1"/>
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    <dbReference type="OrthoDB" id="6021714at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016561">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="term" value="P:axon guidance"/>
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        <property type="term" value="P:dopaminergic neuron differentiation"/>
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        <property type="term" value="P:hypothalamus cell differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="single-minded homolog 1 isoform X1"/>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
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        <property type="entry name" value="PAS domain"/>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
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    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
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    <dbReference type="InterPro" id="IPR000014">
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    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
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        <property type="entry name" value="PAS_fold"/>
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    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
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        <property type="entry name" value="SIM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF22">
        <property type="entry name" value="SINGLE-MINDED HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF06621">
        <property type="entry name" value="SIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
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        <property type="entry name" value="BHLH"/>
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        <property type="entry name" value="SIM_C"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="77"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Single-minded C-terminal" evidence="14">
        <location>
            <begin position="336"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="355"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
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            <begin position="393"/>
            <end position="403"/>
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    </feature>
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            <begin position="420"/>
            <end position="429"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="537"/>
            <end position="548"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="772" mass="86558" checksum="65995543197E3AB5" modified="2014-04-16" version="1">MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRIVFPEGLGEAWGHPSRTSSLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVADLTGYEPQDLIEKTLYHHVHSCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWIQSYATIVHNSRSSRPHCIVSVNYVLTETEYKGLQLSLDQVTSNKPSFSYANSTPTITDSRKPSKSRVARTKSKARASPYPQYSGFHTERSESDHESQWGGSPLTDSASPQLLDHGEGPHSQHPDVSCAYRQYSDRSSLCYGFTLDHAARLSEERHSHYHSQACDGGRCEAGRYFLGTPQPARESWWSTSRSLLTVPKSSPENREGFDNCMPHIASVHSVHVRGHWDEDSVVSSPDPGSASDSGDRYRSDHFQSSPHEPSKIETLIRATQQMIKEEESRLQLRKANQDHLAPLNGVAKTHSSCFGTDFPQTQPQGVVCRGPAVTSSALCDHLQHRDRKMISPHDNEQDNSPTSLSRISSPSSDRISKSGLSLTKDYLQSDLSPHQTHGNHCTSSPSYYSSHHRQYFDKHAYSLTGYALEHLYDAETLRNYSLGCNGSHYDVTSHLRMQQDPAQGHKGTSVIITNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKR8</accession>
    <name>W5NKR8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKR8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021227"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021263.1">
        <property type="protein sequence ID" value="ENSLOCP00000021227.1"/>
        <property type="gene ID" value="ENSLOCG00000017175.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683844"/>
    <dbReference type="KEGG" id="loc:102683844"/>
    <dbReference type="CTD" id="221458"/>
    <dbReference type="eggNOG" id="KOG4280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157697"/>
    <dbReference type="InParanoid" id="W5NKR8"/>
    <dbReference type="OMA" id="DFDLWYQ"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017175">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000039">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056524">
        <property type="entry name" value="KIF6/9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF67">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23735">
        <property type="entry name" value="KIF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="5"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="518"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="609"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="696"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="640"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="757"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="770"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="826" mass="93026" checksum="6663FD4172704270" modified="2014-04-16" version="1">MVKQTIQIFARVKPAKRSTAVYSVDHDEHGSSSVEFLVPRDLADGFINNKKESYRFRFQKVFDQGAKQEDIFENIAKPVADSVLAGYNGTIFAYGQTGSGKTFTITGGPERYSDRGIIPRTLSYLYQHFSKDSSKVYTAYISYLEIYNDCGYDLLDPRHEAARLEDLPKVTIMEDPEQNIHLKNLSIQQSASEEEALNLLFLGDTNRMIAETPMNQASTRSHCIFTVHLSSREPGSATVCRSKLHLVDLAGSERVSKTGVGGQLLTEAKYINLSLHYLEQVIIALSEKNRSHIPYRNSMMTSVLRDSLGGNCMTTMIATLSVDKRNIDESISTCRFAQRVALIKNEALLNEELDPALMILRLKKEIQSLREELAMVTGEQRTDQLTEDDFHMLQEKVALFLQDSDPEAALDLGPDLRKIQHCFRLLKGMVTEQQCNQGRGDEHPSSLHQADQKDQDCPTPMEMKRLRELLQQRDNEITILVNMLKREKKRAQDAVSQLELGSNGFVISRSSYRTSDAENAEGSWESSNPRQTSGSISSVSTVPHPLHKRREVEMSLGRKEAFEIFRRDHEDRINIEDNKALLKQRFAEAKALGEQVNKARGRVNELKAQLEQRRTRRAAHGVTGGTADPAETDPVEEQLRAQIEEEKKNYKATFGRLKALKTEIEHLQLLLERAKVKLQKDFEEWWSEEAYRLQQAPPSEGLGQTSGWTPQEDAPRFQPKPLITRESSVTSGASEKEFPVRGVAEPYSPSARDLRNFNPSLSSTSRGSADRSALVSQSSSLSSELRTPGYTESSIPLTGDQQTDADILAFVRARQNLLQRTGSSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKW5</accession>
    <name>W5NKW5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dual specificity protein phosphatase 23</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Low molecular mass dual specificity phosphatase 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01029904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKW5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021274"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021310.1">
        <property type="protein sequence ID" value="ENSLOCP00000021274.1"/>
        <property type="gene ID" value="ENSLOCG00000017207.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161329"/>
    <dbReference type="HOGENOM" id="CLU_047330_4_2_1"/>
    <dbReference type="InParanoid" id="W5NKW5"/>
    <dbReference type="OMA" id="PAHYQYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017207">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14504">
        <property type="entry name" value="DUSP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000063">
        <property type="entry name" value="Dual specificity phosphatase 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="12"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="80"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="150" mass="16665" checksum="3CADB9717846A1EB" modified="2014-04-16" version="1">AAVVMASLTPPNFSWVEPKKLAGMALPRMPAHYQFLLDNGIKHLVTLCERKPPYHDSCPGLTLHHIKIDDFCPPSMDQIKRFLAIVEEANAKGEGVAVHCLLGHGRTGTMLACYLVKKRKITGVDAIAEIRSIRRGSIETYEQEKAVVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL60</accession>
    <name>W5NL60_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Erythrocyte membrane protein band 4.1 like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL60"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021369"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021406.1">
        <property type="protein sequence ID" value="ENSLOCP00000021369.1"/>
        <property type="gene ID" value="ENSLOCG00000017276.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3527">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155617"/>
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        <property type="component" value="Linkage group LG1"/>
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    <dbReference type="Bgee" id="ENSLOCG00000017276">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
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        <property type="term" value="C:plasma membrane"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
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    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Band_4.1_C"/>
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        <property type="entry name" value="Ez/rad/moesin-like"/>
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        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="SAB_dom"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF41">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 2"/>
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        <property type="entry name" value="Second domain of FERM"/>
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        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="215"/>
            <end position="496"/>
        </location>
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            <begin position="1"/>
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            <begin position="9"/>
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    <evidence type="ECO:0000259" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="980" mass="108875" checksum="3D2E408046C285E0" modified="2014-04-16" version="1">MTTEVGTEAELKRESEKPDELPVTPEEQTAAPETQPPGEAAEALDPPTPQQTSPAAERKEREQAAGKGLSRFLPAWLRKPKSPSQAEPTESSPAEEDGGEGGEQKQKSPPPSPSAPQPEGKEEQSGEGAGVPPAEDRHEQEELKADEKEEKHSISSAETQPAKEERREDTLDKDTDLKEEVKDVGGDKKPEEHAQKPALNADKPSVKPPKKMKMVTCKVTLLDGTDHPFEIEKRAKGLILFQKVCEHLNLLEKDYFGLAYKDNTDQKCWLDPSKEIKRQIRNSPWQFAFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHSLLGSYTLQAEQGDYDPEEHHLDYTSDFQFAPSQTKEMEEKVIELYKTHRGLTPAQADAQFLENAKKLSMYGVDLHHAKDSEGVDIMLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKIRPGETEQFESTIGFKLPNHKAAKKLWKVCVEHHTFFRLVAPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPYFERTSSKRVSRSLDGAPVVNIADQSLPPDAAGKADGVAGRESGDAGTLPRTALELSQESKTKSLRVKGENIYVRHSNLMLEDLDKTQEDVLKHQASISELKRSFMESSPEPRPNQWEKRLTATLPLQTQGSSDDKQKPETCEQAEVEIEETVVIEEIRRTPKVKTGAQAAVSPEIGDSSVKQEKEERRASTSSGSHSESEDEAEYHPEVTGVSPDQVKAGPEEKGSQQESVHAAETPAGEVAVPLSSSDAAVEEDVEEPKDQEHEEEDGRTETEELPEAATVEEPRVNGDMFHGETEHIPQVICCSEPPVVKTEMVTISDAFAAQKMEISTKEVPIVHTETKTITYEASQLDGGGEGEPGILMTAQTITSESLCTTTTTHITKTVKGGLSETRIEKRIVITGDADIDHDEALAQAIKEAKEQHPDMSVTRVVVHKETELTEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMJ2</accession>
    <name>W5NMJ2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box D2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635124.1">
        <property type="nucleotide sequence ID" value="XM_006635061.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMJ2"/>
    <dbReference type="FunCoup" id="W5NMJ2">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021851"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021889.1">
        <property type="protein sequence ID" value="ENSLOCP00000021851.1"/>
        <property type="gene ID" value="ENSLOCG00000017747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688996"/>
    <dbReference type="KEGG" id="loc:102688996"/>
    <dbReference type="CTD" id="2306"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157140"/>
    <dbReference type="HOGENOM" id="CLU_040357_5_0_1"/>
    <dbReference type="InParanoid" id="W5NMJ2"/>
    <dbReference type="OMA" id="NLNTCHY"/>
    <dbReference type="OrthoDB" id="5402974at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017747">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20046">
        <property type="entry name" value="FH_FOXD1_D2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF361">
        <property type="entry name" value="FORKHEAD BOX PROTEIN D4-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="85"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="85"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="356" mass="38693" checksum="B8A559C4F49CEA69" modified="2014-04-16" version="1">MTLGTEMSDNSVLSEDVDIDVVGDMSVVKDGKYHDDFHSDNDSDDNSSRNAGEGAPSPALSGSECTSARGGGGGGEKAGKNTLVKPPYSYIALITMAILQSPKKRLTLSEICEFISNRFPYYREKFPAWQNSIRHNLSLNDCFVKIPREPGNPGKGNYWTLDPESADMFDNGSFLRRRKRFKRHQSTEILRDPNSFLPGFGYGPYGYNYSIQLQNYHHHHGGAFPFQPPQCALPSPASIFQAPHIPSLLGSELTRKPFYPQLSPTVPSLQPLKADSSGQARPSFSIDNIIGVSSAASTPASPYPSQTGSQAQILAVLTPALTSASSHLNLPHENLLQSAGGQAFSSKITNLNSCHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMW0</accession>
    <name>W5NMW0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ovarian cancer G-protein coupled receptor 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NMW0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022008.1">
        <property type="protein sequence ID" value="ENSLOCP00000021969.1"/>
        <property type="gene ID" value="ENSLOCG00000017866.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SPVS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164014"/>
    <dbReference type="HOGENOM" id="CLU_009579_23_0_1"/>
    <dbReference type="InParanoid" id="W5NMW0"/>
    <dbReference type="OMA" id="PWEDSIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017866">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="47"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="33977" checksum="35B88174A218735E" modified="2014-04-16" version="1">QHSILKMDSLNFSNTTEGNSTDDNKLLKMKTFHLVIYSCFFVVVLPINFLALYGLFRLIKTDFPLPVYIINLLLSDVTRIAILPLFIDSTSNDYECPSHCGAGIMTYTLCVVAGVGFLLCISVERYVALAHPLWYRYHRSPKSAVLISLSVWVLGIAFMVGELIGSTIAYWLFLVVIFPLPLLVLVLSCAGIRRAVGRAVSVPEEEKTRIRRLMILLLGIFTGMYGPIFFCYSYFSAAAGEDAAYSNVLEVCLLTAWAIMNLSPLVDPILYIFLRRDVRDALGCLSLCLRPADCRRPPPARQGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMZ9</accession>
    <name>W5NMZ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 229A</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM229 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMZ9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022047.1">
        <property type="protein sequence ID" value="ENSLOCP00000022008.1"/>
        <property type="gene ID" value="ENSLOCG00000017905.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUNK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010899"/>
    <dbReference type="HOGENOM" id="CLU_048581_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMZ9"/>
    <dbReference type="OMA" id="FHLVFYP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017905">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="327" mass="38354" checksum="9AC3ADA915545E9B" modified="2014-04-16" version="1">SRLPQKDLSSTEKAGKSMQKLPAWMRLYFYGMHGITLDIILSSAQIFVKTNDFKMLGFSSPFLCVVHSMTHFALEKVYLQRKSFHRCPVVFHCILYPSVYIFLHILVEKNVASLNHVKRVSVAHLAVQYLIALYYAYLFHKKFLRLQYHCLGSNQAELILERSLRGLPGIIRFVFFGMHGFLDEVIFTSIFNLIENDDRTLTGHTSLWSFLMYGSCSFVVEKLYLYLHYKRGWNAWKRLPIYIGFVYTWEFSWGLILRQYNACSWDYSHYPLNFKGLITLLYLPGWICLSLYQDILSNILLRVECAEGKNTQTCVINGQHRVKNHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPA2</accession>
    <name>W5NPA2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPA2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022461"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022502.1">
        <property type="protein sequence ID" value="ENSLOCP00000022461.1"/>
        <property type="gene ID" value="ENSLOCG00000018360.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161664"/>
    <dbReference type="HOGENOM" id="CLU_1307299_0_0_1"/>
    <dbReference type="InParanoid" id="W5NPA2"/>
    <dbReference type="OMA" id="PMERGSH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018360">
        <property type="expression patterns" value="Expressed in liver and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="229" mass="24663" checksum="2E6EAD81ED306ED6" modified="2014-04-16" version="1">METSKQGCLSAPAPSKQLVQPTSSSVTVWGEDRMHSMQPPMPTVYLNTLGLPMYPQLQPLPSQDSVTIHLSVPQLHESRGSVDPKQSLPTLTLSIVNGMPMLQQGQRLAGTVAGKPRSAGKHVCEHCGRDCLKPSVLEKHLRCHTGERPYPCTTCGIAFKTQSNLYKHRRTQAHARLSSESEKGGSSSQEGSQGSTETCPESHSLGSLEGPQSEESGSFEKESATCPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV88</accession>
    <name>W5LV88_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LV88"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000045"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000045.1">
        <property type="protein sequence ID" value="ENSLOCP00000000045.1"/>
        <property type="gene ID" value="ENSLOCG00000000039.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="HOGENOM" id="CLU_006842_0_4_1"/>
    <dbReference type="InParanoid" id="W5LV88"/>
    <dbReference type="OMA" id="NDSMICA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000039">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF127">
        <property type="entry name" value="SERINE PROTEASE 27-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="37"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="318" mass="34116" checksum="99692AB2B872B0D9" modified="2014-04-16" version="1">TRAPLTTPVTPSITYPTPPPTPIAPNECGKVPLNPRIVGGNPAQEGSWPWQASLHYYGQHMCGGSLINQQWVLTAAHCISETSELKVYLGRQTQAGPNPQELSKKVKQVILHPGYSSSNNDIALLELRSPVTFTNYIQPICLAANSSSFYTGTSCWVAGWGFTSEFMSLPGNQTLQEVQLPIIGNRQCGCLYDLNYGPGSVNDSMICAGQPNGSKDTCRGDEGGPLICKQGSSWVQAGIVRPGEGCGQTGLPGLYTRVSQYEDWIYQMVGMATPGFVTFSSNGSDPERNFTCSPPSSTPISSSDITVIMISPSLRISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVD8</accession>
    <name>W5LVD8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVD8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000095.1">
        <property type="protein sequence ID" value="ENSLOCP00000000095.1"/>
        <property type="gene ID" value="ENSLOCG00000000084.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT3N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010118"/>
    <dbReference type="HOGENOM" id="CLU_024117_3_0_1"/>
    <dbReference type="InParanoid" id="W5LVD8"/>
    <dbReference type="OMA" id="DLGMRYD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000084">
        <property type="expression patterns" value="Expressed in larva and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08586">
        <property type="entry name" value="PI-PLCc_BcPLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF113">
        <property type="entry name" value="SI:DKEY-266F7.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="19"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="269" mass="30520" checksum="E5FB95C8011CB3D8" modified="2014-04-16" version="1">DWDSTPDALWPNWMADISDSKSVAELSIPGTHNTMAFYGGALVECQRWPLSQQYKAGIRFVDIRCRHIENNLTIHHDVVYQQAILGDVFRDTIAFLKENTGEAILMRIKKEYDETGCTQPFDKTLKNKIEAYGQSNFWVKNQIPTLGEARGKIVIIENYPCASVGINYWSLDIADDYHVPTIFHTPQKWSGVKDHLEKARYGSCSQIFLTFASGSSGGAYPNAVADRVNPELYYYLNGFGDQTIRWGIIVMDFPASILVHKVLFLQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW89</accession>
    <name>W5LW89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000396"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000396.1">
        <property type="protein sequence ID" value="ENSLOCP00000000396.1"/>
        <property type="gene ID" value="ENSLOCG00000000361.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_988793_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW89"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="282" mass="32104" checksum="7F4760A48E193BA1" modified="2014-04-16" version="1">QVITVHVYNPFVRNDDIFLFLSLYVDVVSDCTRVVDRLGVWTGRRQFAVILRPYPTSLDGFKHPPASFALGSDRGYLFYAGQPKTCRRCQETGHTADTCSQVRCRNCNELGHLMRDCKKGLIXIVWRGRCRKNRDTKNVWVHMFNPHVPEADIVTFLQRYMDIHGEGQKIINKEGDWTCKRQYMVRFHASDVEVGGVMSPPANFNIRPNKGYVLYPGHPRTCCRCGQLGHISVDCTTEMCRRCGRAGHVAAVCKNPLVCNLCGEQGHSYRMCLKKARSFASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWQ7</accession>
    <name>W5LWQ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein Rab-1</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Small GTP-binding protein rab1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Protein transport. Probably involved in vesicular traffic from ER to Golgi.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_006643280.1">
        <property type="nucleotide sequence ID" value="XM_006643217.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LWQ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000564"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000565.1">
        <property type="protein sequence ID" value="ENSLOCP00000000564.1"/>
        <property type="gene ID" value="ENSLOCG00000000512.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682683"/>
    <dbReference type="KEGG" id="loc:102682683"/>
    <dbReference type="eggNOG" id="KOG0078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165742"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="InParanoid" id="W5LWQ7"/>
    <dbReference type="OMA" id="KVGENTD"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000512">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001018">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867243" description="Ras-related protein Rab-1" evidence="12">
        <location>
            <begin position="19"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="22641" checksum="2F7E5E8BF02412CB" modified="2014-04-16" version="1">MDRMADFLLLKIILVGDALVGKTCILQRYVDHSFDHSYVSTIGIDFKVKTLHVNGRFIKLQIWDTAGQERFHTISTRYFRGAHGLLIIYDITNVQSFDNVVGWTKDVDTNAGENVDRIILGNKCDLEDKRVVSKEKGQQLAWEHGMKFLETSAKENINIEKAFSILVEDILKKNHSNSQRLDVVNLKKSEKKRCCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWZ5</accession>
    <name>W5LWZ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FMR1 autosomal homolog 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoplasmic ribonucleoprotein granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LWZ5"/>
    <dbReference type="FunCoup" id="W5LWZ5">
        <property type="interactions" value="1023"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000652"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000654.1">
        <property type="protein sequence ID" value="ENSLOCP00000000652.1"/>
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    <sequence length="683" mass="76545" checksum="021579E72C3963EF" modified="2014-04-16" version="1">MEELTVEVRGSNGAYYKGFVKDVHDDSLTIVFENNWQPERQVPFSDVRLPPPPDAKKEIGEGEEVEIYSRANDQEPCGWWLAKVRMMKGDFYVIEYAACDATYNEIVTFERLRPVNLNKPVTKNTFFKCTVPVPEDLRVACENELAHKDFKKAIGACRIFYSVEIGQLVILSTNEATIKRVNILSDMHLRSIRTKLMLMSRNEEATKHLESTKQLAAAFHEEFTVREDLMGLAIGSHGSNIQQARKVPGVTAIELEEDSGTFRIYGESAEAVKKARSYLEFIEDSAQVPRHLVGKVIGKAGKVIQEIVDKSGVVRVRIEGDNDNRHPREEGMVPFTFVGTKESIGNVHVLLEYHISYLNEVEQLRMERLQIDEQLRQIGMGFRPSSSRTAEKDKGYTTDESTSASSLHGSRSYSGRGRGRRGPNYTSGYGTNSEQSNASETESDRKDELSDWSLAGEDRDRELRHQRDSRRRPGTGRGRGGTGGRGRAAGRGSAISSISSVLKDPDSNPYSILDNTETDQTADTDASDSHHSASRRRRSRRRRTDGDATVIDGMTESDSASVNENGIVDDLEAKPQRRHRSRKRRLRPPAEDRQPVTVADYISRAESQSRQNPPKDNLKKNKKEMGQVDLIEEHSTLEKMINGPSNGSGDELGKFQCTPTERTQKVTYQGDGNNQETVLNGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX09</accession>
    <name>W5LX09_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LX09"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000666"/>
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        <property type="gene ID" value="ENSLOCG00000000606.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OMA" id="RISHIVC"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="197"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="234"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
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            <begin position="87"/>
            <end position="232"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="260" mass="29744" checksum="955372CF8420E82B" modified="2014-04-16" version="1">QFIVFLFSSLKLEHYTKLFIIFLILFLVTFFSNFFILMLVILDNPAPHLLPSFPSCYQFFSGYDNTISLPSCFAQMYFYISLGAVEIFLVAAMAYVAVVKPHHYNIIINPKICIKMAAIAWMFGFLAPFLSVVFASSLKFCGSNRISHIVCDYPTVKSLACGDDYDYDYENYCHDCNIQYIPFIFVLCSYCRIVWAVTKMKTIESRKKAFSMCSSHVTVVFLYYVSAAMVFIGLRAEGIPHNVCIFFRAMCNCLTPLVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXU5</accession>
    <name>W5LXU5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Atlastin 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034222">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069036480.1">
        <property type="nucleotide sequence ID" value="XM_069180379.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069036481.1">
        <property type="nucleotide sequence ID" value="XM_069180380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXU5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000952"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000956.1">
        <property type="protein sequence ID" value="ENSLOCP00000000952.1"/>
        <property type="gene ID" value="ENSLOCG00000000849.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697332"/>
    <dbReference type="KEGG" id="loc:102697332"/>
    <dbReference type="CTD" id="25923"/>
    <dbReference type="eggNOG" id="KOG2037">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158566"/>
    <dbReference type="HOGENOM" id="CLU_021447_2_0_1"/>
    <dbReference type="InParanoid" id="W5LXU5"/>
    <dbReference type="OMA" id="QYQKNME"/>
    <dbReference type="OrthoDB" id="7788754at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000849">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.420:FF:000001">
        <property type="entry name" value="Atlastin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000314">
        <property type="entry name" value="Atlastin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="13">
        <location>
            <begin position="40"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="514" mass="56865" checksum="6BADF53D3A3F1861" modified="2014-04-16" version="1">MGGASEPRPVQIVSVHKEDHSFSLDAKALEAVLLRPEVRDLDVAVVSVAGAFRKGKSFLLDFMLRYMKRKDEAGDWLGSDTEPLTGFSWRGGSDPETTGIQIWSEVFVVEKPNGKEVAVLLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFLKPFQSLMFLIRDWSFPYEYSYGLKGGMQFLDKRLQVKDAQHEEIQTVRKHIHSCFSNVSCFLLPHPGLKVATSPSFSGQLSDIAAEFKSELQTFIPLVLQPQKLMEKEINGAKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEANNLAAVAAAKDLYYKQMEKVCGGDLPYVAPESLEEKHAFHCAEALELFGRTKKMGGATFSERYRQQLAGDLEQLWADFQKHNQSKNVFSAFRTPAVLFVLLAGLYLLSGLAGFVGLALVAGLCNCGIGLALAAMLAWALIRYSGQYREVGQAIDSSAGFMLEKATVVINSTRGAAVQQGKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY68</accession>
    <name>W5LY68_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="W5LY68"/>
    <dbReference type="FunCoup" id="W5LY68">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001075"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001079.1">
        <property type="protein sequence ID" value="ENSLOCP00000001075.1"/>
        <property type="gene ID" value="ENSLOCG00000000963.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164842"/>
    <dbReference type="HOGENOM" id="CLU_048061_4_0_1"/>
    <dbReference type="InParanoid" id="W5LY68"/>
    <dbReference type="OMA" id="CFRINNC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000963">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="161" mass="18222" checksum="F2A2CA5560023AFE" modified="2014-04-16" version="1">CFRINNCVGELNQKYFIQFLFYTGLASLYSIALVVSAWVWRIRSEREGEGAREEDGGPSKHLIVAHYIILLVESVLFGVFVTVIFYDQLVSIITDETPIEQIRNRLMKDKAAGPHQKRKPKLLLLREVFGRGSVLCWLFPLRSGPPSSGGISYSALPDYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZI4</accession>
    <name>W5LZI4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Potassium voltage-gated channel subfamily Q member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009374">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009375">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009376">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZI4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001541"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001546.1">
        <property type="protein sequence ID" value="ENSLOCP00000001541.1"/>
        <property type="gene ID" value="ENSLOCG00000001349.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159209"/>
    <dbReference type="HOGENOM" id="CLU_011722_8_3_1"/>
    <dbReference type="InParanoid" id="W5LZI4"/>
    <dbReference type="OMA" id="YATCLHM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001349">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF7">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="177"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="17">
        <location>
            <begin position="459"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="434"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="694" mass="77168" checksum="C75962282FB6B777" modified="2014-04-16" version="1">MVGSPSNNGGMRVLSPPAAAGDRKLEFVALTAVHTERSEPSTPERGPLSHRTGLLGTPLPVPPGSRPNGSSSSKRYRKLQNCLYNVLERPRGWAFIYHAFIFLLVFGCLVLSVFSTIPEHQKFANEGLFILVCHHGAWVRGAWYSLSPWRRGQGCVHRSAPPPLMLFCPVLPACDVSDFIVFVASVAVIAAGTQGNIFATSALRSMRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLVLIFASFLVYLAEKDSNTEFSTYADSLWWGTITLTTIGYGDKTPRTWLGRVLAAGFALLGVSFFALPAGILGSGFALKVQEQHRQKHFEKRRMPAANLIQAAWRLYSTDVNHSYLTATWYFYDSMLPSFRELTVLFSHLQRQRSAKKVLPISHHTLLSGLRPYGSPCLPGDSGKMGFRDRIRMNNSRSQALRSKASPAAPTGPAVPTGGRRSPSSESVPEATSPGRVQKSWSFNDRTRFRTSLRLKPRPSADVEAPAEDGAEDKAPHCDVTMEEVIPAVKTLIRAVRILKFLVAKRKFKETLRPYDVKDVIEQYSAGHLDMLGRIKSLQTRVDQIVGRGAIQPDRKVRSEKGEKTPPELDPLDELSMMGRVVKVEKQVQSIEHKLDLLLNFYTQCLKKGSALSLATMPLTLLEPDLTSDYHSPTDQQELFPSANTLNISRSESSTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2W9</accession>
    <name>W5M2W9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2W9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002727"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002733.1">
        <property type="protein sequence ID" value="ENSLOCP00000002727.1"/>
        <property type="gene ID" value="ENSLOCG00000002329.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="HOGENOM" id="CLU_086545_1_0_1"/>
    <dbReference type="InParanoid" id="W5M2W9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002329">
        <property type="expression patterns" value="Expressed in brain"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF519">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865567" description="Receptor ligand binding region domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="8">
        <location>
            <begin position="78"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="20126" checksum="94B4BAF84243A916" modified="2014-04-16" version="1">MSKSILAFFMLSFSFQLNADSTCRMLEKFGSTGLFKDGNLLIAGVFPVFTTEEDIYVSFKTQPQKAKCVGFDLRAFRWTQAMIFAIEEINKDNSLLPNITLGYRVLDTCASSTLTVRAALAILNGVEASNSTCSSSTPAVVTSQSVDISRVLGPFGITLVKLSNMYIYCRWTFATVLLCYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2X2</accession>
    <name>W5M2X2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2X2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002730"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002736.1">
        <property type="protein sequence ID" value="ENSLOCP00000002730.1"/>
        <property type="gene ID" value="ENSLOCG00000002337.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178524"/>
    <dbReference type="HOGENOM" id="CLU_1165504_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2X2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002337">
        <property type="expression patterns" value="Expressed in intestine and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="26033" checksum="3DF8CB372B7CE375" modified="2014-04-16" version="1">MAALGATLAVLLILSMVIIGLLVFKIYKTKTDWKKLSEASIFRSSIGRGSSANDGMQFVNDGYKNGDDASSVDSKCPDEVDHNIYVNLQSKTQELPEPVSFSAAPALASDLLGDSSSLAGSDKTESEKEVKPILTKERRNEEGYKSVWFKEDIDPNAKEEVVIIPDNPETEAVEDYESLAGDDSDKDPESPTAQRKASLEDGEIKFTKTSAVRFQDPKAHSGDKYTLDTDNEEDNISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M353</accession>
    <name>W5M353_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Golgi membrane protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLM family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M353"/>
    <dbReference type="FunCoup" id="W5M353">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002811"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002817.1">
        <property type="protein sequence ID" value="ENSLOCP00000002811.1"/>
        <property type="gene ID" value="ENSLOCG00000002400.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063675"/>
    <dbReference type="HOGENOM" id="CLU_052047_1_0_1"/>
    <dbReference type="InParanoid" id="W5M353"/>
    <dbReference type="OMA" id="CQGNINT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002400">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026139">
        <property type="entry name" value="GOLM1/CASC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896:SF8">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15896">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 2/GP73-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02084">
        <property type="entry name" value="GOLM1CASC4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="203"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="123"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="425" mass="47524" checksum="43563905DB0B0370" modified="2014-04-16" version="1">MAGLGNSRRGGRSLLLIGALIACTVVLGFNYWISSSRNIELQTRVYELEGNVRRAASERGAVELKKNEFQEQLQRQSEQMNQIESLYRQQLQSAQASWKGEKAVLLLNISSSARTIKTMKDQFNTLFKDMGKLQQELQECQRNQSSLQRKITSDMSQCNAQITEVKEGYLQQLSALKKDALQKEEKITTEICIGVSLQPKAVSTGVKTRATDVKGIEKQEKEGTKQVPVHPRNSSHETEEKPVQTEVQNPKPAELETTEIPENTGSRKNVDVQQPKTVAVKQQDKDEAEKPELTEIKENDLENQKNEGGKDKIEDLENDDHKGNGTEEEVEREQLIQDELEILDDSPNGDLIADAAIKPSVAPLVRSGAFCGDNGKQNNELVMGDELADYNGDEENVGEFEADKQAELAENTGVDQDHQLFNEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3N7</accession>
    <name>W5M3N7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626629.1">
        <property type="nucleotide sequence ID" value="XM_006626566.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3N7"/>
    <dbReference type="FunCoup" id="W5M3N7">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002995"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003001.1">
        <property type="protein sequence ID" value="ENSLOCP00000002995.1"/>
        <property type="gene ID" value="ENSLOCG00000002554.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691857"/>
    <dbReference type="KEGG" id="loc:102691857"/>
    <dbReference type="CTD" id="493869"/>
    <dbReference type="eggNOG" id="KOG1651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159371"/>
    <dbReference type="HOGENOM" id="CLU_029507_0_1_1"/>
    <dbReference type="InParanoid" id="W5M3N7"/>
    <dbReference type="OMA" id="PTWNFCK"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002554">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000049">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013376">
        <property type="entry name" value="Glut_perox_Gpx7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02540">
        <property type="entry name" value="gpx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF7">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="24104" checksum="36082F79D82B87D9" modified="2014-04-16" version="1">MDTLSSYPLKSSVPKARMFLVLFTMTICTAGLFLLQNKLFKPRKNKDFYSLEVRDLRGRTVSLEKYRGKASLVVNVASHCEHTERNYKALQEIHRELGTSHFNVLAFPCGQFGDTEPGSNRDIEAFAKANYGVTFPIFGKIKIMGSEADPAFKYITDSVQKLPKWNFWKFLINPDGHVVKYWKPEEPIENIKQEAAALVREIILKKRVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3P7</accession>
    <name>W5M3P7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 6, regulatory subunit 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642532.1">
        <property type="nucleotide sequence ID" value="XM_006642469.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3P7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003005"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003011.1">
        <property type="protein sequence ID" value="ENSLOCP00000003005.1"/>
        <property type="gene ID" value="ENSLOCG00000002546.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687118"/>
    <dbReference type="KEGG" id="loc:102687118"/>
    <dbReference type="CTD" id="55291"/>
    <dbReference type="eggNOG" id="KOG2073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009899"/>
    <dbReference type="InParanoid" id="W5M3P7"/>
    <dbReference type="OMA" id="NEMRHAD"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002546">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF12">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 6 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="712"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="861"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="894"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="921" mass="103625" checksum="03D4CD0B428F4AF1" modified="2014-04-16" version="1">MFWKFDLHTTSHIDTLLEKEDVTLTEVMDEEDVLQECKAQNRKLVEFLVKPQCMEDLVTFITQEPSEDMDEKIRYKYPNISCELLTSDVAQVNDRLGEDESLLMKLYGFLQNESPLNPLLASFFSKVLSILISRKPEQIVEFLRKKEDFVDLMIKHIGTSAIMDLLLRMLTCIEPQQLRQDVLNWLNEEKVIQRLVDMVQPNQDEDRHSNASQSLCEIIRLSRDQMFQVQGCTEPDPLLATLEKQETVEQLLSNIFDKEKNESAIVSVIQILLTLFETRRPAFEGHLEICPPGLNHPSFSVNQSVLEAIRPQLKHFHQLLLEPPKKNVMKTTWGVLDPPVGNTRLNVVRLVASLLQTNTHIINLELIDLNTVGVILDMYFKYIWNNFLHLQVEICTAMILAIPPAQNENAEINRDAGEEQVRDNVLVKHLFQKCQLLQRILDAWESNEKEQSEGGRRRGYMGHLTRIANSIVHNSEKGPNSALIQQLISDLPDDVRERWEAFTSGPLAETNKKNTVDLVNTHHIHSSSDDEVDFKESGFHQDSSLQQAFSDYQMQQMTSNFIEQFGFNDEEFADQDDVVDIPFDRISDINFSLNTNESANIALFEACCKEKIQQFEDAGSDEEDIWDEKDVTFAPESQRRPRSSGSTDSEESTDSEEEDVKRDPFEPPNTNSDDRMEVDANEAPGWTANFDDIPMDTGSTAVVAQSAAPVTVGSSEGWNSSEPISGKEGGWADFTDFSPEISPKDPLRSSSPVAMETSSETADPLGVNLPAQPEEMERWPVDSTHPQAAPSAKMDAGSDGEEEEDRITETVTNGSMKETVSLTVDAKTETAVFKSEEEKLSTSEDASVKYAVPDTAETDKSVPTSPQLASNCQKQSLKHLEKSKSPSNETLNGPHDEVTAMEEAKTDQRVPSPEAAVNGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3X6</accession>
    <name>W5M3X6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferric-chelate reductase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01037015">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037019">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037020">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3X6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003090.1">
        <property type="protein sequence ID" value="ENSLOCP00000003084.1"/>
        <property type="gene ID" value="ENSLOCG00000002605.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S94W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000051759"/>
    <dbReference type="HOGENOM" id="CLU_1006606_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3X6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002605">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900449">
        <property type="term" value="P:regulation of glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042789">
        <property type="entry name" value="FRRS1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902:SF1">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="28481" checksum="16830BF065C069BA" modified="2014-04-16" version="1">IVKNLTTTNGTATILYNISLAVGNVTNGNVSVIVCPSSSPTSTTSSSTRSSSNSTMSFNTTSTNSTTPLNPTVSSSSNSAVPSATIGALTGSITSDQCGKTKICKAVPKNCNPADTGDCLFMSGKSTLGGVFLELSGLSSGYIATILSSDNITGDAYVCAQAQNKTLRFLRADYNRSALVEKSANDIDTIKGSLNGSILQCSFTAKNILNSRSKAAATPYYVILAVGNFTNDTLSAPDVRLRTDSKVDLGLSSASAPALTAVNGMLILVSGLIFGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4R0</accession>
    <name>W5M4R0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynamin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4R3</accession>
    <name>W5M4R3_LEPOC</name>
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        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL53</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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            <dbReference type="ARBA" id="ARBA00042721"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="108" mass="11808" checksum="B02B0558866C85C7" modified="2014-04-16" version="1">KMAAPRGAVVLKTVKKLVVQFCPFESNVRSVREFLAVVGTEKARSTNANCEVIADVKHDRSDPIVDVTFADGERLVMKAANLSSTEMLSALRSRCLEKDPQAKEGAKM</sequence>
</entry>
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    <accession>W5M4S0</accession>
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            <fullName evidence="2">Sorting nexin-30</fullName>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
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                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
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                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
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                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the sorting nexin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="24"/>
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        <location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="435" mass="49679" checksum="2AA44907B7B86E6C" modified="2014-04-16" version="1">TMSAGTPKSLPSSGQKSIHDISHPLSSEDTILTPSPDLPNTSSIGEKDLSLPNGTPVDTSSPASSSSLLNRLQLDDDLDGETRDLFVAVDDPKKHVSTMETYITYRVTTKTTRTEFDLPEYSVRRRYQDFDWLRNKLEESQPTHLIPPLPEKFVMKGVVDRFSEEFVETRRRALDKFLRRIADHPVLSFNEHFNVFLSAKDLNSYKKQGMALLTKMGESVKYVTGGYKLRGRPLEFAAMGDYLDMFTQKLGTIDRIAQRIIKEQSEYLVELREYGPVYSTWAAAETELQEPLEGVSGCVANCCTALEELTEDMSEDFLPVLREYVLYVESMKNVLKKRDQVQAEYEAKLEAVAFRKEERTGVPTDVEKCQDRVECFNADLKADWDRWQNNKRQDFRQLLTGMADKNIQCYEKCLAAWESIVPLLQDKQDAKGETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M523</accession>
    <name>W5M523_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RIMS-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIMBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003481"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003488.1">
        <property type="protein sequence ID" value="ENSLOCP00000003481.1"/>
        <property type="gene ID" value="ENSLOCG00000002948.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3632">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183203"/>
    <dbReference type="HOGENOM" id="CLU_001979_1_0_1"/>
    <dbReference type="InParanoid" id="W5M523"/>
    <dbReference type="OMA" id="VSAPMPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002948">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12014">
        <property type="entry name" value="SH3_RIM-BP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12012">
        <property type="entry name" value="SH3_RIM-BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12013">
        <property type="entry name" value="SH3_RIM-BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000023">
        <property type="entry name" value="RIMS-binding protein 2 isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000006">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000072">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000643">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000016">
        <property type="entry name" value="RIMS-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057884">
        <property type="entry name" value="FN3_RIM-BP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035753">
        <property type="entry name" value="RIM-BP_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035755">
        <property type="entry name" value="RIM-BP_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057950">
        <property type="entry name" value="RIMB1/RIM3A-C-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040325">
        <property type="entry name" value="RIMBP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF20">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25523">
        <property type="entry name" value="Ig_RIMBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25566">
        <property type="entry name" value="RIMB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="599"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1577"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1717"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="215"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="510"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1143"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1232"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1286"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1670"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="62"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="525"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1143"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1290"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1310"/>
            <end position="1321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1780" mass="196080" checksum="D73C4A6D3BE0BE58" modified="2014-04-16" version="1">MSAKDRDASLIKPAGTGGTNIAALRASILGKRHHSLYEGLNGCEVGGFGKNGTDYGYLVRQNSELLRALDELEKTCAKLREENSLLRKSSSPETEEKVKRLKRKNAELAVIAKRLEERARKLQEANLKVVNAPALVRGGGVEQYKRAFARQRARDLAQHADTLLSKDKEIAALQQECRALQARLGQGKGSPGLLATSEFETLLRESQKEVLRLQRQLSVSSAKEPASGTGQEKAQSENPSPVTDEGPRPPEDTSGLEEQRPSVTPEALSSQPEDQRLQELESELSKKRKECESLEHEVKKRQRKCLDLEHELEDVRGKHASLEEEARRLRLKAQLLKQVQANNDELRDSLSTVTAQRNSALQENQRLQAKLENLEQVLKHMREVAERRQQLELEHEEALTILKLKQEEVKRLQKAQVAAKKEHEGVVQLLENTLDCMQVKVQELEEKCRSQSEQFGLLSHELERFRLQAAKFDLLSSSLDPPSPLTNGLELSGEKDLEVTPPAAHTALAAEPAKEQPLEAEELSVQHQEQPTASSTNSEPEPAAGTPKPDSRQLPPKSESLPHSTPKSCPTPEVDTASEVEELDIDVSPIPEPENRAPAKLQVFIARYSYNPFDGPNENPEAELPLTAGEYIYVYGDMDEDGFYEGELMDGRRGLVPSNFVERISDDDMLSFHPPEVSDLSHNSFQESSFHSSSEKHLRLSIHSTEKTDPSVPEEEENLSGLGKVDSLMSNGFDLDMEEVGDDAVPYPRKLTLIKQLAKSIIIGWDPPLVPAGWGNVWSYNVYVDKELRLNVPFGSQTKAVLERLDVNLKAYRVSVQSVTEKGDSDQLRCSILVGRDVCVAPTQLKVESTTATSASLAWLPSNSNYVHAVFLNDEECEIVKAGCYSHCLSNLRPNLRYRVKVEARPHRTPWELPLDRRERKCAVTSFITLTAGPPDAPLDVQVELGPSPGIALVSWLPVTIDAAGTSNGVRVTGYAIYADKQKVLEVASPTAGSVLVGPSQMHILQAAQELTVRTMSTYGESTDSVPQSFSQPRLPASLCQPHPVPAACWRSACTAPQCVGTPWTPQLPQPLYSLPLLFGLSRQLPSPPAKHPCPPFPKQTSVTAWVLLSTERRGQNSTKDSESVIVKRPSVSITDFLEEAVSSADPSSSVAEMSVESSQGLPGESAGRTEDQVPSGSHSHCLDTSEIDVVLQDPVNDHRLSEYQTESSRGSDLSDILEEDEEDLCSDALGTEEGEEQSGGLGVGDGSKPSASAQPDTWETDSDEEILEKILKLPLQTHHSKQLFSIPEVTEEEDESQELEDGESPDVSETPHHGSEKRPTIPETFHGARKLASGRNPTLLAEANNHRVETGNKASEYQEEDFDSGGSVYIHSKEPRMRRCHEREPKDSHLLAEKSGDRLRREALLQTQRDHISSELLKSGLHAGKYYSSRGGRGNNASVGNGVEIDIEYGTEDDEEALVYRPAAVHLGQMHAGWWVDGTQSEGPEELSDCSSPCESVTSEGRRRELKRQGMVSRSRKRSFTCCVETLLRVPPSALKRLLTKGGSLFLSLIFTSNAPRAETPEATECSEVEGLAADSTVRIFVALFQYDPAVMSPNPDAAEEELPFKEGQIIKIYGDKDADGFYRGESGGRLGYVPCNMVSEIQVEDEETRDQLLQQGFLSAEASMEKIGTRSHAQLPRRPVPPPKPRRSRKGVCCFPLCPSRSGPPCPPGSGPHSVAPRKMVAIFDYDPRESSPNVDIEAELTFSAGDIIVVFGDMDDDGFFYGESNGQQGLVPSNFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M534</accession>
    <name>W5M534_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MORN repeat-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M534"/>
    <dbReference type="FunCoup" id="W5M534">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003492"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003499.1">
        <property type="protein sequence ID" value="ENSLOCP00000003492.1"/>
        <property type="gene ID" value="ENSLOCG00000002968.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684487"/>
    <dbReference type="KEGG" id="loc:102684487"/>
    <dbReference type="CTD" id="254956"/>
    <dbReference type="eggNOG" id="KOG0231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018089"/>
    <dbReference type="HOGENOM" id="CLU_117237_0_0_1"/>
    <dbReference type="InParanoid" id="W5M534"/>
    <dbReference type="OMA" id="NGRMEGK"/>
    <dbReference type="OrthoDB" id="300500at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002968">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042814">
        <property type="entry name" value="Morn5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46437">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46437:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="172" mass="20002" checksum="BC7D3974C92133D3" modified="2014-04-16" version="1">MEYIGSRYEGDFKNRRMEGRGEYTLPTDTRYVGEMKDGMFHGEGTLYFPGGSKYEGTWERGIAKKGKYTFADGLEYKETDWEYCDGYERRFYTEVCDGLKPAGQSQLTNLDPPRVIPEGCYDCGDGFYNPNTRVITDYKNQFLRNADDDEHEWIIRTCRKGWDEIVGHKPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5L5</accession>
    <name>W5M5L5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein kinase 40</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be a negative regulator of NF-kappa-B and p53-mediated gene transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01009501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5L5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003673"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003680.1">
        <property type="protein sequence ID" value="ENSLOCP00000003673.1"/>
        <property type="gene ID" value="ENSLOCG00000003116.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182986"/>
    <dbReference type="HOGENOM" id="CLU_035107_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5L5"/>
    <dbReference type="OMA" id="HGIFKDR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003116">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13974">
        <property type="entry name" value="STKc_SHIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000269">
        <property type="entry name" value="Serine/threonine-protein kinase 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024236">
        <property type="entry name" value="Ser/Thr_kinase_40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF16">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="48"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="472" mass="53656" checksum="29C6910B36DEFF1C" modified="2014-04-16" version="1">MCSFLLERFKRMSKRRASERGAGETSARASKVLCSGISGNNAKRAGPFILGPRLGNSPVPSIVQCLARKDGTDDFYQLKILTLEERIDKGGETQEERQGKMLLHTEYSLLSLLHNQDGVVHHHGLFQDRAYETVEDHEANKIRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETIVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSDNTVCLIRKLLVLDPQQRLTAAEVLESLSSIIASWQSMSSLSGPLQVVPDIDDQVNHPEHLQEAKVTEESSQYEFENYMRQQLILAEEKNTIHEAKSFLHKRQFGNIPPVRRLGHDAQPVSPLDAAILAQRYLRKSHCCELQCSSVTESMCQVCGEEPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6E4</accession>
    <name>W5M6E4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ventricular zone expressed PH domain-containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MELT/VEPH family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6E4"/>
    <dbReference type="FunCoup" id="W5M6E4">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003952"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003960.1">
        <property type="protein sequence ID" value="ENSLOCP00000003952.1"/>
        <property type="gene ID" value="ENSLOCG00000003346.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018660"/>
    <dbReference type="HOGENOM" id="CLU_010394_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6E4"/>
    <dbReference type="OMA" id="WIRIMLL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003346">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042471">
        <property type="term" value="P:ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01264">
        <property type="entry name" value="PH_MELT_VEPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000138">
        <property type="entry name" value="Ventricular zone-expressed PH domain-containing protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039888">
        <property type="entry name" value="Melted-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21630:SF10">
        <property type="entry name" value="VENTRICULAR ZONE-EXPRESSED PH DOMAIN-CONTAINING PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21630">
        <property type="entry name" value="VEPH-A/MELTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="690"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="807" mass="90889" checksum="C41DDC40DAF512DB" modified="2014-04-16" version="1">MHHLFSLVLGHRDLSRAGDLFSLEDAEIEGCLSEALEEIKAISCSPDYLTNDNDQAVVEICITRITTAIRETHSIEKHGKALVSLWESCLEYNLKPEGKDEDTPHAKIASDIMSCVLQNYDRPPVMALAVPVAVKFLQRGSRDLCRNMSSYLSLAAIAKADLLADHTEAITKSVLQGKATFVRFKSRVLLGRPDGCNSFIWEFSSMEKSLSHFYLQKITFLLQLYILHTWELSSSVPSLISYLNDPSVNDSILNVLVEISLCNPSVVAHYLPNLKAVGNEFPNLLGQIAKLYGAVGRTDEEQARSCLVYLVTTLANMEHAFHHTLLLEIKGITDAFSSILGAYSKDVYRMSNSFTAIAKLLVKKLESDRSLSRVEDKAEDPPKACDVNPTVKHDGESEKLQVKIQAFEEKMMSENSTPGPVRRYSLSQAAKEERKEMRFNRSKSLALHVVRSKSMNSEHSQEEDGGDLPAEVCLSERSPSQESCGTAVQGNSSADSVRESCQGGNAQTDALNGQNEDRLCQHLRDNLSKMKAFSAEMVKQIPVPEQCIIEVSDSIRSCVAKLFFSCPMKGQYCLYSKSSFSLSSRQPRVWIQIMLLYRQSMSCEPLASDDKAVQLLKSMWEKTQLKGAHSFETAMMQSAFPHKKDLEALHTRLEEARFYDLFGYSEETGGWLCFMCNNPEKATVVNQDGQPLIEGKLKEKQVRWRFIKRWKTRYFTLAGNQLLFRKGKSKDDLDDSPLELSKVQSVKAVAKKRRDRSLPRAFEIFTESKTYVLKAKDEKNAEEWLQCISVAVAQAKERENRESMTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7R0</accession>
    <name>W5M7R0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MARVEL domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7R0"/>
    <dbReference type="FunCoup" id="W5M7R0">
        <property type="interactions" value="830"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000004426.1">
        <property type="protein sequence ID" value="ENSLOCP00000004418.1"/>
        <property type="gene ID" value="ENSLOCG00000003718.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000049375"/>
    <dbReference type="HOGENOM" id="CLU_138621_0_0_1"/>
    <dbReference type="InParanoid" id="W5M7R0"/>
    <dbReference type="OMA" id="AAHKYEG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003718">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF28">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="179" mass="20240" checksum="2662506D90918774" modified="2014-04-16" version="1">THPSPPLEEKDTAMPPQQQVNRTSLSVKKEFLKSFIGILRVLQILVGAGLWITIAANKYEGSIHFVLFVAVLFWLLTLALYFVTLLDRQDFVPFLGGDRWLLSNTLHDLLATLLFFVALGIMIYKTDKNSFCNLELYKHLCLYKVYLTASVFCCLCAAAYLVSAIYGSCRKCKGKQTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7U7</accession>
    <name>W5M7U7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="26 27">Fatty acid 2-hydroxylase</fullName>
            <ecNumber evidence="27">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="24">Catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. FA2H is stereospecific for the production of (R)-2-hydroxy fatty acids. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-saturated fatty acid + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (R)-2-hydroxy fatty acid + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38855"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:76177"/>
            <dbReference type="ChEBI" id="CHEBI:83955"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:38856"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>docosanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = 2-hydroxydocosanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:39819"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:76722"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:39820"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hexadecanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxyhexadecanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:75927"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:38552"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>octadecanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxyoctadecanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:39815"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57562"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:39816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>tetracosanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxytetracosanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38559"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:75935"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38560"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="29">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27 28">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="27 28">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="25">Sphingolipid metabolism; galactosylceramide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="27"/>
    <dbReference type="EMBL" id="AHAT01010547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7U7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004455"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004463.1">
        <property type="protein sequence ID" value="ENSLOCP00000004455.1"/>
        <property type="gene ID" value="ENSLOCG00000003741.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002142"/>
    <dbReference type="InParanoid" id="W5M7U7"/>
    <dbReference type="OMA" id="WTIIEYV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003741">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120520">
        <property type="term" value="F:free fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000011">
        <property type="entry name" value="Fatty acid 2-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18 27" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 27" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 29" id="KW-0349">Heme</keyword>
    <keyword evidence="15 27 29" id="KW-0408">Iron</keyword>
    <keyword evidence="5 27" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16 27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 27 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 27 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0492">Microsome</keyword>
    <keyword evidence="14 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 28" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="32">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="29">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="29">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051625"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052862"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00053017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054885"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00069158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="382" mass="44355" checksum="B42FF245EAAA31F0" modified="2014-04-16" version="1">FQLSQSPMSSPAPVRYFSESEVSQHRSPSSCWVLLANRVYDVTGFLRRHPGGEALILSRAGTDVSRQMEGPPHRHSENARRWMEQYYIGELRKDPGDTQATETVRERRQGSHQTPEEETGASRCSSVNTETDLVDWRKPLLWQVGHLGEKYDDWVHQPVDRPIRLFYSDVIEACSKTSWYMVPIVWLPVVAYLSWYCFSSLARGDTRLFASFTTDYSIPIHKYTFPFMFLLGMFLWSLVEYLIHRFLFHMKPPASNYYLITFHFLLHGQHHKSPYDGSRLVFPPIPASIVIVAFYGLIRLLLPEALGVSIFVGGLCGYVVYDMIHYYLHYGSPSKGSYMYGLKAYHVKHHFEHQRSGFGITTRFWDHPFHTLIPEETFEKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8M1</accession>
    <name>W5M8M1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tetraspanin 31</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629282.1">
        <property type="nucleotide sequence ID" value="XM_006629219.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8M1"/>
    <dbReference type="FunCoup" id="W5M8M1">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004729"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004737.1">
        <property type="protein sequence ID" value="ENSLOCP00000004729.1"/>
        <property type="gene ID" value="ENSLOCG00000003959.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686463"/>
    <dbReference type="KEGG" id="loc:102686463"/>
    <dbReference type="CTD" id="6302"/>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160351"/>
    <dbReference type="HOGENOM" id="CLU_088363_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8M1"/>
    <dbReference type="OMA" id="AWGIMEN"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003959">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF3">
        <property type="entry name" value="TETRASPANIN-31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="22964" checksum="CF69A051586669F1" modified="2014-04-16" version="1">MVCGGFTCSKNALCSLNVVYMLVGLLLIGVAAWGKGFGIVSSIHIIGGVIAVGVFLLLIAVVGLIGAINHHQVMLFFYMVILFLVFLFQFGVSCSCLAINRDQQVKLLNSTWGIMGNATRQDLETRLNCCGLLNTTEAQAQFLNDVGACHAECKLKASCHTCGEKMLQHSTEALQILGGVGLFFSFTEILGVWLAVRYRNQKDPRANPSAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9P7</accession>
    <name>W5M9P7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tripartite motif containing 42</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9P7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005106"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005114.1">
        <property type="protein sequence ID" value="ENSLOCP00000005106.1"/>
        <property type="gene ID" value="ENSLOCG00000004271.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079104"/>
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    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064220"/>
    <dbReference type="HOGENOM" id="CLU_023245_0_0_1"/>
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    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004271">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16579">
        <property type="entry name" value="RING-HC_PML_C-V"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF229">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="516"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="619" mass="70521" checksum="CC0A6C5A83C93A7D" modified="2014-04-16" version="1">MSEAKDEAETRWWRRGSTERENSGDKTPSTESLPKSPGKQLYLKNLHKKERLPVGSAPGATDSRTELAHLDGSIYQHLTCPSCKNIFSQPCVLPCNHSLCKHCLGAIKKKASRDESNLQFAMCPVCNLLHRCEHNGTFNFPENHLLRNIVKRYSQQSKTRSSKKKQGLAFEEFVNCELCLKDKNISERYCKTCMLNYCKKCLKKLHSNPAFQFHMLTKPVNKEEGELACFYHPEKVLTYYCFTDGATICEECMKVNHISHERAEISEAYINRVKSLNAALEQAGRIKDACEADITDVKLFTCRLKCEGVELLRRVTEGFLSLHKKLTEKEEEITEVIEMLLMKSQEEADQFISYSSKHLSFLEGLVQYSQEAQKERNKAVFLQGIDNTVSEIYTVLDEIYQPNKSLKEEPLKNLTVDFESVMDGLSDLYTKYLIFDKNIKKPNVQRTYSAALTAPSITSIGAAAEVVEKEESQNSIQDWPEENNMRVVSSDTGGNSNSEELELLPAGDRSEDKLHLPRPPVIYQHNVKGVSAEIFWMVPLGEQVDTFDVKFQEIVSIRSTNVLPQDQGEMVFGIKMCSFRAGPLKPKAQYLFTVRALNKYGHGKWSCPYWVMTEDADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBK2</accession>
    <name>W5MBK2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein GBX-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Gastrulation and brain-specific homeobox protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May act as a transcription factor for cell pluripotency and differentiation in the embryo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636368.1">
        <property type="nucleotide sequence ID" value="XM_006636305.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBK2"/>
    <dbReference type="FunCoup" id="W5MBK2">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005761"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005769.1">
        <property type="protein sequence ID" value="ENSLOCP00000005761.1"/>
        <property type="gene ID" value="ENSLOCG00000004803.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682186"/>
    <dbReference type="KEGG" id="loc:102682186"/>
    <dbReference type="CTD" id="2637"/>
    <dbReference type="eggNOG" id="KOG0489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154365"/>
    <dbReference type="HOGENOM" id="CLU_052189_0_0_1"/>
    <dbReference type="InParanoid" id="W5MBK2"/>
    <dbReference type="OMA" id="EDECNRK"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004803">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021549">
        <property type="term" value="P:cerebellum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050935">
        <property type="term" value="P:iridophore differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334">
        <property type="entry name" value="HOMEOBOX PROTEIN GBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334:SF3">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="239"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
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        <location>
            <begin position="138"/>
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        </location>
    </feature>
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        <location>
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            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00060134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00071240"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00076268"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="37448" checksum="EFA401847BEC901A" modified="2014-04-16" version="1">MSAAFSPSLMMMQRPLGSTTAFSIDSLIGGPPQPSPGHFVYTGYPMFMPYRPVVLPPPPPPPPTLPQSALQPGLPPTHPHHQIPSLPSSFCSSLAQGMALTSTLMATLPGGFSSSPQHQEAARKFGSQSLQAVFDKTEDVRPDGDEGKTFLGKDNAVLPPFQASEPLQSSIARAQSKDDTKEDDSNRKDESFSMDSDLDYSSDENAASQTLCQKDDGDPTGLEESGHGSNGTNSTTSTGKNRRRRTAFTSEQLLELEKEFHCKKYLSLTERSQIAHALKLSEVQVKIWFQNRRAKWKRVKAGNVNSKTGEPSRNPKIVVPIPVHVSRFAIRSQHQQLEQARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC10</accession>
    <name>W5MC10_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ETS transcription factor ERG</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003127">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC10"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005919"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005927.1">
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        <property type="gene ID" value="ENSLOCG00000004920.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160662"/>
    <dbReference type="HOGENOM" id="CLU_045216_0_1_1"/>
    <dbReference type="InParanoid" id="W5MC10"/>
    <dbReference type="OMA" id="RVPQQEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004920">
        <property type="expression patterns" value="Expressed in mesonephros and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000010">
        <property type="entry name" value="Fli-1 proto-oncogene, ETS transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000039">
        <property type="entry name" value="Friend leukemia integration 1 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF216">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR ERG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PNT" evidence="10">
        <location>
            <begin position="113"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="309"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="257"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="53394" checksum="089A27EF04FE227F" modified="2014-04-16" version="1">CGSPPQEALSVVSEDQSLFECAYGTPHLPKTEMTASSSSEYGQTAKMSPRVPQQDWLSQPARVTIKMECNTSQVNGSRRSSPDGCGLVKAGKLASGSDGGPMSYGSYMEDKHIPPPNMTTNERRVIVPADPTLWTTEHVRQWLEWAVKEYVLLDVDVSLFQNIDGKELCKMSKEDFQRLTPSYNAEILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGATFIFPNTSVYPDAAPRVSTRPDIAYEAARRSGWTPQAVSSSKGTQPSSSVVTKNEEQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSNCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSMYKYPSDLSYMSSYHAHQQKMNFVAPHPPALPVTSSSFFAAPSPYWNSPTGTIYPNSRHPAAHMPSHLGTYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDG6</accession>
    <name>W5MDG6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="17">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:42713"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="20">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01025162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDG6"/>
    <dbReference type="FunCoup" id="W5MDG6">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006425"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006433.1">
        <property type="protein sequence ID" value="ENSLOCP00000006425.1"/>
        <property type="gene ID" value="ENSLOCG00000005332.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154908"/>
    <dbReference type="HOGENOM" id="CLU_020965_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDG6"/>
    <dbReference type="OMA" id="VARCYEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005332">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016215">
        <property type="term" value="F:acyl-CoA desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045850">
        <property type="entry name" value="TRM2_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025823">
        <property type="entry name" value="TRM2B_chor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904:SF1">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51621">
        <property type="entry name" value="SAM_MT_RNA_M5U_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="4 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="653"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="23">
        <location>
            <begin position="268"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="22">
        <location>
            <begin position="513"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="756" mass="84561" checksum="2DC22182CE7E8A5F" modified="2014-04-16" version="1">SWEERLADAVTPLWRLSYEDQLRVKYEAQRTVLLRLSAQLAQGADPLSPLPWTSGQLGFPLLPILPSPVTDGYRNKSTFSVNRGPDGNPKTVGFYVGTGKARDIVCVRGDHLQNMPAKHKLVARCYEEFIRLSPLEPCLLFHEGGHWREITVRTNAAGSTMAIVYFHPQRLTQEEVCAHKAALTDYFARGPGKVCQLDSLYFQESSGTRCSHEDSPYQLLRGEPHIYEELLGLSFRISADAFFQVNTGGAEVLYRAVGERGFAEGAGTLLDVCCGTGALGLSLSRRARKVVGIEVVEQAVRDARHNAALNGVPNCRFLPGKAEEVLPGLVPSLGSEPDLLAVVNPSRAGLHYRVTRALRNCTALRRLVYVSCKPEGEAMRNFRDLCCPPDGQKKLTGHPFVPTSATPVDMFPHTAHCELLLVFESYSPLLPPLPSRMCVTESMRPHTHRPKNGEVATESTVEDVFDETYREKEGPKPPRKIVWRNVILMSLLHAGALYGLTLVSSASVLTLMWAVLCFLLSALGVTAGAHRLWSHRSYKAKLPLRIFLAFANSMAFQNDIYEWARDHRAHHKYSETDADPHNSGRGFFFAHIGWLLVRKHPDVIEKGSKLELTDLKADPVVMFQRKNYKMSVVLMCFVFPTVVPWYLWGESLWVGYFIPGILSAAHLWGNRPYDVHISPRENRFVTFSAIGEGFHNYHHTFPYDYATSEFGCKLNLTTCFIDFMCFVGLATDCKKVPQELVMARAQRTGDGSHRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME60</accession>
    <name>W5ME60_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Autophagy/beclin-1 regulator 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the DCX(AMBRA1) E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat AMBRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME60"/>
    <dbReference type="FunCoup" id="W5ME60">
        <property type="interactions" value="1470"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006669"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006677.1">
        <property type="protein sequence ID" value="ENSLOCP00000006669.1"/>
        <property type="gene ID" value="ENSLOCG00000005523.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016223"/>
    <dbReference type="HOGENOM" id="CLU_008882_0_0_1"/>
    <dbReference type="InParanoid" id="W5ME60"/>
    <dbReference type="OMA" id="XAVIGDG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005523">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
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    <sequence length="1252" mass="137527" checksum="E1C945D663048308" modified="2014-04-16" version="1">MKVTHQKNSVRILSGRERGAQGFGSQRLLQQLVEEKARCMKWQSQKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGKCIHSLVGHRRTPWCVTFHPTISGLVASGCLDGEVRIWDLHGGSENWFTESNAAIASLAFHPTAQLLLIATNNEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHNLLTAIVNPSNQQNDDDSEIPMDSVEMPHFRQRSLLQSQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPTEESAVPPSLPSGRYTSLRDRPAYQGCVQHLGMVCFCSRCSVARNPPSSPLPGEDAAGSQAAGSEAAPHASTFTSARTEPRLPSEQRGSERPSAFSSVHSSTAGNTLRNLWPGPGRRGLLGLMANQPLGHQQMTRDPSWRLAGTFWNTQSETGDPGSSGGGGMPPPRTSASSVSLLSVLRQQEGGSPSPVYTSATEGRGFHVPADGGGGGSGSGSTTAAAANNNNNGCTVSTSSGHHPLDEASQSSPASIRNVLQCNLSRYFMEYDRMQDMEQAMSGEGTQPQPTEELLNNNIDSDRPGPSHYQPPHSSENSSNLFRGHLNRCRACHNLLTFNHDTQRWERTGQTSSTTSEEGTSWQAPNPAFEGTGGGTREPQPPERRGGAPGPSPATPQGEQTVGLVYNQETGQWERVYGQGAASRPGNVPQEALNQEMPEENSDEDSLRRRLLESSLISLSRYDISGSRDHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSLRYQQNRLRPSSDGPASNPSQSMENNEVEFEEFEDNGDRARHRTPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNATINVLVPNCKIYNDASCDISADGQLLAVFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPTTDHMVAQVFRLQQPHGGETSMRRVFNVVYPMAPDQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPVDSRSESDAQSDHVNEPIFAINNNSGSRNTERAGTSRATWRSDRDLGLMNAIGLQPRHPAPSVTSQGTQTPILQLQNAETQTDRDLQETGTSRATNVCPRHRCVIEHSSIKHHEHPKFYMLLCPKSISKSANVGNLSVKRHLEREGSEYGTSEDALSRIRRLIAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF79</accession>
    <name>W5MF79_LEPOC</name>
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        <submittedName>
            <fullName evidence="11">Zinc finger protein 408</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01030877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000007038.1"/>
        <property type="gene ID" value="ENSLOCG00000005829.1"/>
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        <property type="component" value="Linkage group LG27"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
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        <property type="entry name" value="PRDM17_PR-SET"/>
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        <property type="entry name" value="SET_dom_sf"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
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        <location>
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        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="591"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="591"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="686" mass="76350" checksum="CFA772419D18E312" modified="2014-04-16" version="1">CIRSSSPDPESPCPALLRTLPPGLALGPSLARGSCIGVWCVGKAVHKGAFFGPLENCAHPTGVDETEEQGRGPNSEQTIDPLPLEEAGNWIRFACRAGSEGEWNACVLRLRGRPYVKALEDIEPGVELLLRPGEEGEVSAEEGQGTRGQEAQSKDETVKTEERHARGEALDSETEEAPRLPGNNAGRRSPEINNSSHNIDPKESHEQAVPKPQGAHKDSRNHLPRDSEETDSAQIDEDGSPEALNNPGASSDRPSKAAQGDDGDSVTETPQVEGAHKEPEEGGRIGEEGTGGFRASLRLAAKPRKASDARKRKYKCDECEKCFFQLCHLKKHKFTHRDYKPYLCNECGKSYSSQESFRAHVLQHKGERPFKCQHCDKTYGLKRDLKEHEVLHTGERPFSCDICGKSFARRPSLRIHKEIHRMKELNVKVSKACKCTVCDKELANSGSLKNHMRLHTGERPYPCSHCGKTFRQRGNLLGHLRIHTGEKPYKCEYCEQRFSQIPELRRHLISHTGEVYLCPVCGKALRDPHTLRAHERLHSGDRPYKCDQCGKAYTLATKLRRHQKSHLEEKPYKCQTCGAGYTLMQSLVRHQLSHKRKTSRRPRADKKASVQPQGVVGSSTETGQTVQGQARGHIQLNDDIIEIIISDSHNKCIIVEEDKSHSNVVILQEDEGLNSVAETVEIETGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFX8</accession>
    <name>W5MFX8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex I-30kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">NADH-ubiquinone oxidoreductase 30 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. Interacts with NDUFAF3. Interacts with RAB5IF. Found in subcomplexes containing subunits NDUFS2, MT-ND1 and NDUFA13.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFX8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007287"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007295.1">
        <property type="protein sequence ID" value="ENSLOCP00000007287.1"/>
        <property type="gene ID" value="ENSLOCG00000006031.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017480"/>
    <dbReference type="InParanoid" id="W5MFX8"/>
    <dbReference type="OMA" id="LRFNSWI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006031">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.80:FF:000002">
        <property type="entry name" value="NADH dehydrogenase iron-sulfur protein 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01357">
        <property type="entry name" value="NDH1_NuoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="13">
        <location>
            <begin position="81"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="25470" checksum="9B12741EB2322701" modified="2014-04-16" version="1">MAASLVRFVRGGFSRSFNFATRSAAVVPARSEGTATETRPTVRPKDEVTHKQLSAFGEYVAEILPKYVQQVQVTCFNELEVMIHPDGVIPVLTFLRDHTNAQFKSLADLTAVDIPSRQNRFEIVYNLLSLRYNSRIRVKTYTDELTTVDSAVPVHQAANWYEREKKIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVEVGSVSRSQPVSPLLGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG29</accession>
    <name>W5MG29_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG29"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007338"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007346.1">
        <property type="protein sequence ID" value="ENSLOCP00000007338.1"/>
        <property type="gene ID" value="ENSLOCG00000006078.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173222"/>
    <dbReference type="HOGENOM" id="CLU_179694_0_0_1"/>
    <dbReference type="InParanoid" id="W5MG29"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006078">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="69" mass="7684" checksum="2EBD9D3885F03512" modified="2014-04-16" version="1">MAAAAALERSYIEICGFERETLQKFRDITVDPGVNALPGGVKYPDSAGGFHYEECDKLLSVTSNRFIQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIN7</accession>
    <name>W5MIN7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rhotekin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIN7"/>
    <dbReference type="FunCoup" id="W5MIN7">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008246"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008256.1">
        <property type="protein sequence ID" value="ENSLOCP00000008246.1"/>
        <property type="gene ID" value="ENSLOCG00000006821.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688034"/>
    <dbReference type="KEGG" id="loc:102688034"/>
    <dbReference type="CTD" id="219790"/>
    <dbReference type="eggNOG" id="ENOG502QRWR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157470"/>
    <dbReference type="HOGENOM" id="CLU_025066_1_0_1"/>
    <dbReference type="InParanoid" id="W5MIN7"/>
    <dbReference type="OMA" id="GNHKMVI"/>
    <dbReference type="OrthoDB" id="5817051at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006821">
        <property type="expression patterns" value="Expressed in ovary and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13249">
        <property type="entry name" value="PH_rhotekin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012966">
        <property type="entry name" value="AHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF21">
        <property type="entry name" value="RHOTEKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08174">
        <property type="entry name" value="Anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="4">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="313"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="645" mass="71882" checksum="E42FD7B9745A074A" modified="2014-04-16" version="1">MDQRRDIQNFKRNERTRVTVSSCSSLGMEIKRKKIKESTVILQQEDCDLQEKIDFEIRMREGAYKLLVASTQRDQVLSASKNLLTCNARIEAYMAELQKKGEVHILINAARRSSDGVPKDRIACKGKVAISGLRIPLMWKDTDHFNNRGSSRRVAVFCLMKMGAEVFDTDMVIVDRSVTDICFDGLTIFKEASPDFELKLEVYSCAMEEETSLVNTPKKLAKKLGSSLSKSTGKKHCPLQEGGDPESFFQSSLQALGAKYSLLAHTTLGLAQAEEGFQSHTLTVPPNEDSSFWLPLYGNVCCRLVAQPECMTQDMMSGFLNQQQNIVGVPRCSRLFCVLRAGQLLCYYTPEEIEAKLDPAIVIPINKETRIRAIDKDSKKRSHSFTIINPRAGEAVTNVFVAECKEELQKWMEGFWQHFYDLSQWKHCCSDLMKIEVVSPRRPPLFLTKQATSVYHDLSIDSPTKLESLTDLIHHKIEETGGRFLIGQEEDPVAPRWASLFEGSHPMVVQKCVLSPARGSETPSPGAAAAKKRRAPPPPPAKAVALGPAASHALPREEAGSWRRPAVRAGRPSLDSKLSAVIQQLQRPSVPSRKKPPRHQEGTGAENRPPELPARPVPAPRPRLSSLKEKMKPKPRIPALDGVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJN2</accession>
    <name>W5MJN2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Endosomal/lysosomal proton channel TMEM175</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Potassium channel TMEM175</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 175</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJN2"/>
    <dbReference type="FunCoup" id="W5MJN2">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008591"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008601.1">
        <property type="protein sequence ID" value="ENSLOCP00000008591.1"/>
        <property type="gene ID" value="ENSLOCG00000007095.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015667"/>
    <dbReference type="HOGENOM" id="CLU_052593_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJN2"/>
    <dbReference type="OMA" id="FFFPVSY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007095">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31462">
        <property type="entry name" value="ENDOSOMAL/LYSOSOMAL POTASSIUM CHANNEL TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31462:SF5">
        <property type="entry name" value="ENDOSOMAL_LYSOSOMAL PROTON CHANNEL TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="57727" checksum="F639AC0EE48FA973" modified="2014-04-16" version="1">MEKKSTRTTDPSSFLQSVTLSEKDGHSSTQSSHRLLAFSDSLISIIATVMILPIAHTKIQQDQELQKSLQQLLATKISVYLVTFLIVTVAWAAHIRLFQVIERIDDVLALLNLACMMLITFLPYTFSLMASFPENPLGILLFCGCAIVIGLIQAGIVLYGFSQPYLLIDHIQISENQNYYKYQILKVILRVPVFCFLAGIFAFIFFPLAYILLALVIFYPYLSQTLKWCCNKALGQQEEHNETMLLYTYHPKEPLSKERVEAFSDGVYAIVATLLILDICEDNVPEPSEVKERFNNSLIEALHDFGPEYLAYFGSFVTVGLLWFVHHSLFLYITKTTRFMGLLNTFSLAFIGGLPLAYQLTHEFEEKSHNELEAIQISCVIIFFAGIFQMAIWVTALFNERETLHPYVRYRGKEHAFMFAKLSIYPCVSLSTFFLTCILSEFSTPIFHLMEIIVPFAFLALRILVRIALALLKLVFCPSKPIHNSVEEDETRLPFNDVLWYIIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJX7</accession>
    <name>W5MJX7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox even-skipped homolog protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">EVX-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJX7"/>
    <dbReference type="FunCoup" id="W5MJX7">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000008696.1">
        <property type="protein sequence ID" value="ENSLOCP00000008686.1"/>
        <property type="gene ID" value="ENSLOCG00000007179.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161025"/>
    <dbReference type="HOGENOM" id="CLU_045075_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJX7"/>
    <dbReference type="OMA" id="NKGYAES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007179">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021521">
        <property type="term" value="P:ventral spinal cord interneuron specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000367">
        <property type="entry name" value="homeobox even-skipped homolog protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF1">
        <property type="entry name" value="HOMEOBOX EVEN-SKIPPED HOMOLOG PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="195"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="444" mass="47527" checksum="045C245E92183400" modified="2014-04-16" version="1">MMERIRKEMILMERGLHSPAAAKRLSNLSDSAGNAVLEALENSQHSGRLSPRLTSASLHGNLGDIPAKGKFEIESLFNPHHPNNDNTSSSEISSSENRKKIALYPEVAQEADMNSDVEVGCSAHRSPNSLSATQHKENNSKDKTTHGFLMFTYTVSFLVFSDSTSATSNTSTSSVSSLNINGNSIGSSSSSADQVRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMSWPHPADPSFYTYMMTHAAATGSLPYPFHSHMPLHYYPHVGVTAAAAAAAATGAAASPFATSIRPLDTFRALSHPYSRPELLCSFRHPGLYQSPAGLNSSAAASAAAAAAAAAAAAGAPSGTGPCSCLSCHSSQAASALGSRSASADFTCTAAGQRSESGFLPYSAAVLSKTAVPSPDQREEASLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKH4</accession>
    <name>W5MKH4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008883"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008894.1">
        <property type="protein sequence ID" value="ENSLOCP00000008883.1"/>
        <property type="gene ID" value="ENSLOCG00000007329.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158337"/>
    <dbReference type="HOGENOM" id="CLU_001582_1_0_1"/>
    <dbReference type="InParanoid" id="W5MKH4"/>
    <dbReference type="OMA" id="QKCTICT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007329">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000770">
        <property type="entry name" value="Agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000031">
        <property type="entry name" value="NtA agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000134">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000140">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000027">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000045">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000008">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000018">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000022">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000024">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003884">
        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF83">
        <property type="entry name" value="AGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00057">
        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="5 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="17 20" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="27">
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            <begin position="159"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="232"/>
            <end position="286"/>
        </location>
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    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="303"/>
            <end position="357"/>
        </location>
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    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="377"/>
            <end position="431"/>
        </location>
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    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="441"/>
            <end position="501"/>
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        <location>
            <begin position="519"/>
            <end position="566"/>
        </location>
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    <feature type="domain" description="Kazal-like" evidence="27">
        <location>
            <begin position="604"/>
            <end position="652"/>
        </location>
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        <location>
            <begin position="693"/>
            <end position="746"/>
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    <feature type="domain" description="Laminin EGF-like" evidence="26">
        <location>
            <begin position="747"/>
            <end position="799"/>
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            <begin position="811"/>
            <end position="867"/>
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            <begin position="1013"/>
            <end position="1135"/>
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            <begin position="1230"/>
            <end position="1266"/>
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            <begin position="1271"/>
            <end position="1447"/>
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            <begin position="1448"/>
            <end position="1485"/>
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            <begin position="1487"/>
            <end position="1524"/>
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        <location>
            <begin position="1534"/>
            <end position="1717"/>
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        <location>
            <begin position="1713"/>
            <end position="1749"/>
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            <begin position="1760"/>
            <end position="1938"/>
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            <begin position="872"/>
            <end position="898"/>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1152"/>
            <end position="1240"/>
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        <location>
            <begin position="1152"/>
            <end position="1200"/>
        </location>
    </feature>
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            <begin position="693"/>
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    </feature>
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            <begin position="695"/>
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            <begin position="714"/>
            <end position="723"/>
        </location>
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            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
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        <location>
            <begin position="749"/>
            <end position="766"/>
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            <begin position="768"/>
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            <begin position="1256"/>
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            <begin position="1475"/>
            <end position="1484"/>
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    </feature>
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        <location>
            <begin position="1514"/>
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        </location>
    </feature>
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            <begin position="1739"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
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            <dbReference type="ARBA" id="ARBA00016077"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000008883.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1941" mass="208719" checksum="AFAA52E1DFFB4FB3" modified="2014-04-16" version="1">MTSCQYPTPSPDRDRMYQHKVSLVVRYFMIPCNICLILLATSTLGFAVLLFLNNYKPVHFTPAQPPDGCRGMLCGFGAVCERDPADPSKGECVCKKTCPPVVAPVCGSDSSTYSNECELEKAQCATQRRLKVVRKGSCAKDPCSEVTCSYGSTCVQSSDGQSAKCMCPLSCEQEPLHRVCGSDGQDYPSECQLNQHACRLQKNIRKEHDGPCDPCQDSRSNPNVACRVEPRTRRPQTFASPESCPADREPVCASDGQTYDNKCRMERTGKLQGLELKVLHAGRCRKEDECPEGCPFNAVCQVERSGPRCSCEAIQCDGTYSPLCGTDGRTFGNNCERRRAECQARAYIPVKQGPCGLHNPSPCQDKPCGFGAACVVRNGEPVCECPDACPKVHDPVCGDDEHTYGSQCEMKAMGCVLQREIRIRHKGPCEGEEREPCSSCRFGAICDTETGRCVCPTECVASHQPVCGTDGATYANECELHVQACTQQKDLRVAAQGECKTCGDGVCSWGAMCVRNQCVCPSCAGQPGAPVCGSDGVTYDSTCDLRAASCHLRKKIDIARTGPCDEECGSSGGSGSGEDSACEQERCKKFGGSWDEDAEDDRCVCDFTCQSVPRSQVCGTDGRTYSSECQLKKTRCEKQAHVEIHSQGPCAATPSTAAPTASAQHCSQAVYGCCLDNTTFALGVGLAGCPSTCLCNSYGSYGGSCDPTTGQCSCKPGVGGPKCDRCEPGFWNFRGIVTDNMSGCTPCNCNPTGSVRDDCEQMTGLCSCKTGVTGMKCTMCLDGGASCEKGPAGQASCAALQCRFGATCAEVNGKAQCECPPPDCAEKNKTKVCGSDGVTYADRCQLRTIACRQATAISVKHVGQCSDSLGCRARGSTSSPTRHPQGPGGPRVDCSRSSGAECASVPWRVFRVKASPSLFTCRSLLLPPETIVTPAQAARPLMISRGFCQSPIPGLRGRSAGVLTLRVPLPEPAESTTALPAPEDRSSCDNAPFGCCPDSRTAASDSEGTNCPPTMKFSGFLHLEEVEGQEVFYTPEMDDPKSELFGETARSIESALNELFRKSDVQKDFKSVRVRSLAPSSSILAFVEAHFDPDTQYTVEDIENALLTQLKASKTKAISVKKPEKDNIRFSNFGLVSLPFFTTTATTTATVTMPTTTTPLPTATATTRRPSTTSRATTRRPYSNRRTTTAAPATARRPPTTTTPPVPSSTSPPLTTPPVRARLVPQPKKPPKPCESHPCRHGGTCEEDGRDFTCICPAGRGGAVCEKVIRYYIPSFGGKSYLAFTTMKAYHTMRIAMEFRASELNGLLLFNGQNGRKDFVSLALASGFVELRFNTGSGTGVLTSRVRVELGKWHQVVVTRNRRSAMLSVDSEAHVTGESPPGTDGLNLDTDLFIGGVPEELLADVRERTSASAGLVGCVRALDVNNMLYNLQENGGEVLYGSGVGECGNNPCLPNPCRNGASCLAKEAEMFHCKCVHGFSGPTCADAHNPCEPNHCHPSAQCQVLPQGGYKCVCPMGREGTHCERESRSGARETFMPFFNGDSYLELKGLQSYGHDLQQKVSLTLVFLAKDPNGMIFYNGQKTDGKGDFISLALSDGFLEFRYDLGKGPAVIRSKERIEMDVWNTVSLERASRKGEINVNDKDRVRGESPNQHTALNLKESLFIGGAPDFSRLARAAALKDGFKGAVQKITLMGTPVLKRANVLNSVDISMYQPHPCSQSVCQNGGLCRPLLEVYECVCQHGFTGGRCENAIIEKSAGEVEAIAFDGRTFLEYHNAVTKSEKALLVNRFELSIKTEATHGLLLWSGKGVERSDYIALALVDGRVQMTYDLGSKPVVLQSTVRVNTNRWVRIKASRALRDGSLQVDNEAPVTGSSPLAATQLDTDGTLWLGGLEQLAVARKLPKAYSTGFVGCVKDVVVDGLELHLVEDALNSPKILHCSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML49</accession>
    <name>W5ML49_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML49"/>
    <dbReference type="FunCoup" id="W5ML49">
        <property type="interactions" value="1745"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009108"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009119.1">
        <property type="protein sequence ID" value="ENSLOCP00000009108.1"/>
        <property type="gene ID" value="ENSLOCG00000007504.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260040"/>
    <dbReference type="HOGENOM" id="CLU_058290_1_0_1"/>
    <dbReference type="InParanoid" id="W5ML49"/>
    <dbReference type="OMA" id="AECKVFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007504">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10022">
        <property type="entry name" value="14-3-3_beta_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="6">
        <location>
            <begin position="5"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="246" mass="27931" checksum="4DCB16AE1B5B73F8" modified="2025-10-08" version="2">MKMDKSDLVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGNEKKQQMAREYREKIEAELQDICNDVLGLLDKYLIANATQAESKVFYLKMKGDYYRYLSEVASGDSKKTTVENSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEQACSLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSENQGDEADAGDGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM87</accession>
    <name>W5MM87_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tropomodulin 4 (muscle)</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM87"/>
    <dbReference type="FunCoup" id="W5MM87">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009496"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009507.1">
        <property type="protein sequence ID" value="ENSLOCP00000009496.1"/>
        <property type="gene ID" value="ENSLOCG00000007811.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158734"/>
    <dbReference type="HOGENOM" id="CLU_031052_0_1_1"/>
    <dbReference type="InParanoid" id="W5MM87"/>
    <dbReference type="OMA" id="SDAEMCD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007811">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014866">
        <property type="term" value="P:skeletal myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF9">
        <property type="entry name" value="TROPOMODULIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="348" mass="38997" checksum="7798BDDD02EE0C99" modified="2014-04-16" version="1">AARMSSKSKENYRDIDEDAILKGLSAEELEQLECELQEMDPENAMLPAGMRQRDQTKKSPTGPFNRDALLEHLEKQALELADREDLVPFTGEKKGKPFIPKQSKREIPREEQITLEPELEEALANATDAEMCDIAAILGMYTLMSNKQYYDALCCGNIVNTEGINSVVKPDKYKTYPDEPPNPTNVEETLRKIKSNDQSLTDVNLNNIKDIPIPTLKEIFEGMKTNTSVRSLSIAATRSNDPVAFAAAEMLKVNKSLQSLNIESNFITGEGMMAVMKAMRENSTLTEIKIDNQRHSLGDSVEMEIAAMLESNPSIVKIGYHFTQQGPRARAAIAITKNNDFLRQQRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMT4</accession>
    <name>W5MMT4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Semaphorin-1A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMT4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009693"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009704.1">
        <property type="protein sequence ID" value="ENSLOCP00000009693.1"/>
        <property type="gene ID" value="ENSLOCG00000007977.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3611">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165314"/>
    <dbReference type="HOGENOM" id="CLU_009051_11_2_1"/>
    <dbReference type="InParanoid" id="W5MMT4"/>
    <dbReference type="OMA" id="PGMCVRK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007977">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000016">
        <property type="entry name" value="Putative Semaphorin-6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000223">
        <property type="entry name" value="semaphorin-7A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF85">
        <property type="entry name" value="SI:CH211-113G11.6 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866339" description="Semaphorin-1A" evidence="13">
        <location>
            <begin position="21"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="2"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="634" mass="69471" checksum="EE5950A1C0FA8CE8" modified="2014-04-16" version="1">NRVFKAFTLFFLVSCKRTLTCFVEPGRFIFPKPENYTTVLHQDGSDVIYIGGQAVVYALNFTDLRAKDPKILVTLDENAKEACLGKPTVLKEQCENFITVIQRVNDSLVVCGTNAGSPKCWLMVNDTELSDTTTNGQMMAPADISPPFPSQRVVSLSAEGNLYSALSATGRQGGFIRRAYGSQKLLKSEDKWLHNPQFAGAAVIPAMPRHLEEIYFFFSEINRTATPDEDPYRARIGRVCLVDEGGSKTVLPDSWTTFLKARVRCGVEGTPQQYNRFKEAYVLTSTDKKVGVLYGIFSNAWDITVVCAYSIEEIDQSFGTSKLKGYTSPLPAHRPGTCAFKNVTSGLAAKTLGVIRDHPEIEDVIRPLGKQPLALLTSDHFTKMAADTVLAVNDEHYSVLYLGTGKGKVLKVLHTIEEVFIISQYSLFAAEAPVSLMTIDSHKGHLYVGTSLEVQRLPLADCQRYGNTCRDCVLSRDPYCGWDAAGRQCTAIPPGYNSSFGSILQNLDQSNTSVCGDSADLKAPSTDPKEVLVNPDGPILLPCPVRSYHASYTWEKDNCQKRYPCSINGGSCVLAPTPDLPLKDGVFRCMALEDGHKEEVVSYRLVLNSAIPAGPARASLLPSLLVAAAALWLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNM2</accession>
    <name>W5MNM2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-kinase anchor protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01007601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNM2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009993.1">
        <property type="protein sequence ID" value="ENSLOCP00000009981.1"/>
        <property type="gene ID" value="ENSLOCG00000008219.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SBE6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204498"/>
    <dbReference type="HOGENOM" id="CLU_1207208_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNM2"/>
    <dbReference type="OMA" id="SEGMACK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008219">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042779">
        <property type="entry name" value="MISP/MISP3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18839:SF0">
        <property type="entry name" value="MITOTIC INTERACTOR AND SUBSTRATE OF PLK1 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18839">
        <property type="entry name" value="MITOTIC INTERACTOR AND SUBSTRATE OF PLK1 MISP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25008" checksum="3E1E379F118EA8F0" modified="2014-04-16" version="1">LSGTAPSVNLEPKGPSLTEGAVGKVIILENTIRIPATLLRAAPVANDPARSHEGERAPVLDSRGMPYAPQGEEEPDGEVLLEDEPPRENPFFKLRSSMSLKPEVEQDIREAQQRERELRRQRFSLYGTAGGPRDSEEPQPATPNGLAIPSTPPPNRPVSPSPSPSPAPSARQSLGKLDVTWPPPCRPAAEPSGHTEEFPDVKGSPKTPRQKIPLLQRWETGMVNGHQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNQ8</accession>
    <name>W5MNQ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PDS5 cohesin associated factor B</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MNQ8">
        <property type="interactions" value="1511"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010017"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010029.1">
        <property type="protein sequence ID" value="ENSLOCP00000010017.1"/>
        <property type="gene ID" value="ENSLOCG00000008252.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157257"/>
    <dbReference type="InParanoid" id="W5MNQ8"/>
    <dbReference type="OMA" id="YPPAYNM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008252">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140670">
        <property type="term" value="F:cohesin unloader activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF1">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1138"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1138"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1157"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1173"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1199"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1228"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1320"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1343"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1420"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1445" mass="164911" checksum="E7A0A48DA07D5BFB" modified="2014-04-16" version="1">MAHSKARATDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSAQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFQVINNGHNQKVHMHMVDLMGSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSVSDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNDERLQVVKLLAKMFGVKDSELAAQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDLSLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYSLQGEAGKEATKQISWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETAERMKCLYYLYATLDFNAVKALNEMWKCQNMLRHHVKDLLDLIKQPKSEASSKAVFSKVMVITRNLPDPGKAQDFVKKLAQVLEEDERIRNHLETLVSPTCSCKQAEGCVREITKKLGSPKQPSNPFLEMVKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEDEGVPTEQAIRAGLELLKVLSFTHPVSFHSAETFESLLGCLKMDDEKVAEAALQIFKNTGSKMEESFPHIKSVLLPVLQHKAKKGPQRQAKYAIHCIHAIFSNRDTQFAQIFEVISKAMPFFKNKDQHRTVFLHKEEKLKRDEQCDVPETFVQLCFVFVQLCCRDSVPESRWGNKGYLHMKQWVVSVLFQIQGIKLMVRWLLGVKNNSSKSGNSTLRMLTAILHSDGDLTEQGKMGKPDMSRLRLAAGCAILKLAQEPCYHEIITLEQYQLCALVINDECYQVRQAFAQKLHKGLCRLRLPLEYLAIFALCAKDPMKERRAHARQCLVKNINIRREYLKQHAAVREKLFSLLPEYVVPYTIHLLVHDPDYVKVQDIEQLKDIKECLWFVLEILMAKNENNSHAFIRKMVENIKQTKDAQGPDDSKLNEKLYTVCDVAMNIIISKSTTYSLESPKDPVLPARFFTQPDKNFSNTKNYLPPEMKSFFTPGKPKAANVLGAVNKPLSTAGKQSQTKSSRMETISNASSNSNPSSPGRSKGRLDSTEMDHSENEDITIMKSSPMPGKKNDKRDDSDLLRQDGEKPRGRKRAAASSQDDRSGLEDSAQEQKPKRGRKRPANSSAGEAEEQQWAEEKRIQDGTLETEDEQNSPPKKGRRGRPPKSVSGGAAKEETPSKGRRGRKKAAAPAEEEAEEDEEEEDRNSENMEVKQKGGRQARTPRRSQQRVEASEATDEAVESTPQKRRGRPPKAAQSLPKKNARGSRSRAAASKENDSADEMEVSQASIEDTAQEEEEDEDEEAEEVRRKTARRDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPQ9</accession>
    <name>W5MPQ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Spectrin repeat containing, nuclear envelope family member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPQ9"/>
    <dbReference type="FunCoup" id="W5MPQ9">
        <property type="interactions" value="1012"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010368"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010381.1">
        <property type="protein sequence ID" value="ENSLOCP00000010368.1"/>
        <property type="gene ID" value="ENSLOCG00000008520.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039367"/>
    <dbReference type="HOGENOM" id="CLU_012764_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPQ9"/>
    <dbReference type="OMA" id="ACCLEDQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008520">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045111">
        <property type="term" value="C:intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057932">
        <property type="entry name" value="Spectrin_SYNE1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057933">
        <property type="entry name" value="SYNE3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF2">
        <property type="entry name" value="NESPRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25803">
        <property type="entry name" value="Spectrin_SYNE1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25804">
        <property type="entry name" value="SYNE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Nesprin-1/3 spectrin repeats region" evidence="8">
        <location>
            <begin position="336"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Nesprin-3" evidence="9">
        <location>
            <begin position="890"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="838"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="251"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="840"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="995" mass="115498" checksum="DA7AC9CEF183A7E6" modified="2014-04-16" version="1">MTQQEWDEFGESLESAQAWMQAIQERLQISDNTQGPRTELEARLRETERIYESQHEGRMKMDRVLVAGEALLQCGDEDTKQETFTKLKEVKDLWEETYTYIIHCHSRIEWVWLHWSEYLKALEEFRQWLAKTNSALECQLELQLGVQEKLWQLDHHRILLSDTENQALLLERLMEEAATLHSRTQDPTVGEEVLKGLKTSYEDIRTRSQERVLLLTKVVEQHQLYQSLAEKFRMWLLSKCEELSCYNEKTKDGTEEKLKELEELYRSVNREEKTVQEIETLAESVQANTSPQGSRRIAEEVEDLRQSWERLLQSCVHKQERAKVTLQSQAEHMAMASQLRENIAELWNRVLELSRQLEAQDGERSEEQMMVTWRRYMATRMALAEEESSIERLKAQLKGLFRLSQDTKPLSDEVLAVVKEYQSVKSKAFRLSMEMEASLRRVLQDPLRDFSQWSPSVTRTLDASADVADSSLISVHLRNIEELLLQSSLLQQQLHLLHSKQDLLSSVFSEEKADSLRLELSEAVKEREHLHSKLLQRKSKLQGLMLRTKDIDDVYGIILQKQADIRVRLAIGDELQPDVQAKTRQHNQLQAIQEDLEDVKTHIEALKSQSSSNPSDKNKISQFLAENKALSIALQDRILASRQNIWEHKNFHENLRSLEKWLMITRQKLESHGNTEEHWSADHHRAETEKVLAEFPEREIELHHLEAQGESVLAKTSPAGRELIWSNLERLKESWKILHTLSMTPSRQDGSASSLRLSYCPSSFQNDSLIWSFSNHFGMSLGGYVLTYSIDAQFFRITRKVLYLSQFTILEVQKNCKILTSATPDCFSRISDLLAPARRVAPERESPDGARPIDQKYLSAPSTSNNGREVQYAISLEVSQETPRNVDFIKLQKKFEEWLQGENHKLTRILSRRGFLSGKELKIRQKGLKDLHSHIAHGQSLFQQLMTSSGSRGVGDAELEELRYRWMLYKSKLKDSRNLTASVGPKVKYKHRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ87</accession>
    <name>W5MQ87_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01007549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ87"/>
    <dbReference type="FunCoup" id="W5MQ87">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010546"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010561.1">
        <property type="protein sequence ID" value="ENSLOCP00000010546.1"/>
        <property type="gene ID" value="ENSLOCG00000008674.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="HOGENOM" id="CLU_049894_10_2_1"/>
    <dbReference type="InParanoid" id="W5MQ87"/>
    <dbReference type="OMA" id="WICERRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008674">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="46"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="17651" checksum="F17201B0AB3B2897" modified="2014-04-16" version="1">TREHGGSVFISVSQMSSRLNSLTSQLQAQGSTGGCTKSCSSDWILFSGNCYYFSKETRDWSGAQQYCGNQDSGLAIINNDDELTHLSSKISSIHWLGLTDLDREGSWKWVDGTPVDKKYWNLGEPNNAGEEDCGELKEAKLNDGRCSEIKRWICEKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQT5</accession>
    <name>W5MQT5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 29 member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQT5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010759.1">
        <property type="protein sequence ID" value="ENSLOCP00000010744.1"/>
        <property type="gene ID" value="ENSLOCG00000008830.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182898"/>
    <dbReference type="HOGENOM" id="CLU_021611_6_1_1"/>
    <dbReference type="InParanoid" id="W5MQT5"/>
    <dbReference type="OMA" id="GSPWTTK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008830">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <sequence length="468" mass="51135" checksum="AFF753381498199D" modified="2025-10-08" version="2">MRMEGSETLQASVNSSYLNAAESLDDDQDPLLPSEAEASPRFPGTQRPHDSYNLVYAIFFLLGVGSLLPWNFFITAKHYWVYKLTNASGQTDGQVPRSNLSDYFESYLSIASTVPSVLCLVLNFLLVNKVSPAVRVLSSLAVILLVFMVTTVLVKIDTTERREEFFVVTLVSVAVVSGTSNIFTGSVFGVTGRFPMRISQALISGQAMGGTLSAVASLIDLAAAHDITDSALAYFLTADVFILLCIFAYLLLPHLHYSRYYMLPPPCGVPGNDGACAEDQQARDTGGVPPLRPILKKTAVLGLCVFYVFFISIMVFPAVSSGIQSVHKESGSPWTNEYFVPLTSFFLYNAADFCGRQATAWVQVPGPRSRLLPSLVLLRTALVPLFMFCNYQPRDHLQTVFFAQDVYPVVFISLLGLTNGYLGTLPMIYGPKVVRRDLAEAAGVVMSFFLMLGLAIGSAFSILIVHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR40</accession>
    <name>W5MR40_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-noradrenaline(out) + chloride(out) + Na(+)(out) = (R)-noradrenaline(in) + chloride(in) + Na(+)(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:72587"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>dopamine(out) + chloride(out) + 2 Na(+)(out) = dopamine(in) + chloride(in) + 2 Na(+)(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dopamine(out) + chloride(out) + Na(+)(out) = dopamine(in) + chloride(in) + Na(+)(in)</text>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000008899.1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 21" id="KW-0769">Symport</keyword>
    <keyword evidence="6 21 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
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            <name>Na(+)</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="625" mass="69919" checksum="617A9F547FA0BD1A" modified="2014-04-16" version="1">GAKMTVKKMHPLKRLPVAQMSSVVAPAKQSNTMGPKEVELILVKEQNGVQFTNSTLINPAPTSPGNEERETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLFFMVIAGMPLFYMELALGQYNREGAAGVWKICPIFKGVGFTVIIISLYVGFYYNVIIAWALFYLFSSFTGKLPWVDCNNTWNSVNCSDLWSFNSSTLNDTHKSTPAAEYFERGVLHVHESEGINDLGLPRWQLTSCLVVVIVILYFSLWKGVKTSGKVVWITATMPYVVLTVLLLRGVTLPGAIDGIKAYLSVDFLRLCEASVWIDAATQICFSLGVGFGVLIAFSSYNKFSNNCYRDAIITSSINSLTSFFSGFVIFSFLGYMSQKHKVALDEVATDGPGLVFIIYPEAIATLPGSSVWAVIFFIMLLTLGIDSAMGGMESVITGLIDEFKFLHKHRELFTLFIVVTTFLISLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGIAWFYGVDRFSDDIKEMIGHRPGLYWRLCWKFVSPCFLLFMVVVSFATFNPPYYGTYVFPVWANMIGWCVAISSMTMVPIYAIYKFCVLPGTFRDKLAYAITPEKEHHLVENGEVRQFTLHHWLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRT6</accession>
    <name>W5MRT6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="22">NudE neurodevelopment protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01042702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637450.2">
        <property type="nucleotide sequence ID" value="XM_006637387.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRT6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011111.1">
        <property type="protein sequence ID" value="ENSLOCP00000011095.1"/>
        <property type="gene ID" value="ENSLOCG00000009097.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698564"/>
    <dbReference type="KEGG" id="loc:102698564"/>
    <dbReference type="CTD" id="54820"/>
    <dbReference type="eggNOG" id="KOG1853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000111"/>
    <dbReference type="HOGENOM" id="CLU_057872_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRT6"/>
    <dbReference type="OMA" id="MVEPTTH"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009097">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007100">
        <property type="term" value="P:mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF2">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="21">
        <location>
            <begin position="156"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="202"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="41700" checksum="E87B882FCB406025" modified="2014-04-16" version="1">MQPQSWNISFHNRATAWRRSAMTEAVAPKLCSLEEELRYWKEAAEKYRQSAEEAQDELQEFQQMSRDYEQELEAELKQCEARNRELLTHNNRLRMELETYKEKFEAQHSEAFRQISTLEGDLAETTAVRDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRMNQVIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQERRPSLSAAAKEGEKAESPARPPSALPSLPGTPGGPRMTPSTFATPPSSYRRGDGLTGTPLTTSARISALNIVGELLRKVGNLEAKLASCRDFVNDQSPGRLAPCNRAGSAAPSRESPESKAAPNCLPLFDKGLAKRLEFGPAPSSVASQTAQSPQGVVKILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS75</accession>
    <name>W5MS75_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aspartyl-tRNA synthetase 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS75"/>
    <dbReference type="FunCoup" id="W5MS75">
        <property type="interactions" value="1018"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011234"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011250.1">
        <property type="protein sequence ID" value="ENSLOCP00000011234.1"/>
        <property type="gene ID" value="ENSLOCG00000009206.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
    <dbReference type="InParanoid" id="W5MS75"/>
    <dbReference type="OMA" id="LCGWVDR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009206">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00777">
        <property type="entry name" value="AspRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04317">
        <property type="entry name" value="EcAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047089">
        <property type="entry name" value="Asp-tRNA-ligase_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047090">
        <property type="entry name" value="AspRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="200"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="661" mass="74514" checksum="17644A33A2E0091B" modified="2014-04-16" version="1">MSYKAGILIRRILMKGIKWHHSARTNSTATSYRQCSQRSMTGKYLAVMRPSAILTGHNSFSSRSHTCGELRASHAGEQVTLCGWIQYQRQDLFVILRDFHGLTQLFIPQDESGAQLKEVLCDLPLESVVRVTGEVRQRPPGQENKEMPTGDIEVCVRSVEVLNACRRLPFEIKDFVKKSESLRMQYRYLDLRSVQMQYNLRLRSQMVMKMREYLCNLHVGFVDVETPTLFKRTPGGAKEFIVPSREPGRFYSLPQSPQQFKQLLMVGGIDRYFQVARCYRDEGSKPDRQPEFTQVDIEMSFVDQDGIRTLIEGLIQHSWPEDKGQIHVPFPCMTYAEAMKYYGTDKPDTRFEMKLVDISEAFKHTAIGFLQDALNKPQGCVHAFCVPNGATHFKNKDLEVLRELAKTQFNQEVSAVVVKSDGIWKSPLSKLLTESEKQQVCQMTHARAGDLVLLSAGQPQDVCPALGKLRLHCAELLEARGVGIRDPSAFHFLWVVDFPLFLPKEDEPGQLESAHHPFTAPHPLDADLLYSQPHKVRGQHYDLVLNGCEIGGGSIRIHNAKLQQYVLEETLKEDKGLLSHLIEALESGAPPHGGIALGLDRLISIIVGAASIRDVIAFPKSFRGHDLMSNAPDFVPAEDLKPYHISTTWPAGAQKNKGQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSJ6</accession>
    <name>W5MSJ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermatogenesis associated 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211423.1">
        <property type="nucleotide sequence ID" value="XM_015355937.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069050404.1">
        <property type="nucleotide sequence ID" value="XM_069194303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSJ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011355"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011371.1">
        <property type="protein sequence ID" value="ENSLOCP00000011355.1"/>
        <property type="gene ID" value="ENSLOCG00000009309.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688997"/>
    <dbReference type="KEGG" id="loc:102688997"/>
    <dbReference type="CTD" id="54558"/>
    <dbReference type="eggNOG" id="ENOG502QRV3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063821"/>
    <dbReference type="HOGENOM" id="CLU_038272_2_0_1"/>
    <dbReference type="InParanoid" id="W5MSJ6"/>
    <dbReference type="OMA" id="HGREFDD"/>
    <dbReference type="OrthoDB" id="5963614at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009309">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042769">
        <property type="entry name" value="SPATA6_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032732">
        <property type="entry name" value="SPATA6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435:SF3">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14909">
        <property type="entry name" value="SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 6 N-terminal" evidence="4">
        <location>
            <begin position="25"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="54611" checksum="6395C8B13C737F81" modified="2014-04-16" version="1">MGGLTAQNRIYRSCKMPHKVLKCIIELEIQAVTCPGVVLTDQEDIYLSVCIMGKYQKTKCLPPIFPLLFHEKLNFEKVFADAVDPGDIAELLELDTTSFELIQLVPPVGETLATLVENTREFLYPGPRLTPRYPGSEREMLMKRSISFPGIAPKVEFSTVSVIKECDLKETMQTPSPCRSTPLKPRSGRGIKKSPCSADQQSPSVCRYKQPTVASQSRSPSPYTNRRMCELSEEASQRLSHLNLGPYRFKKETDSQPPFVVRNVSSKQTSSMNDSLSYRTPSTKKSLRTPGLNVCEDPSLLGSYRPKTTRVIAEPQEGYRLRPSRGMSEDTSLETAGICRRPRRQLKAHSTPLSTVKSPPSPLLNRSSLRERFQTSPSTPTHWEEIHERVQKILKTHRSRHGMTPVWGSLTDSVHQKTSTSCNDSLCDSQVQEDSSRGLGEATVHLDNGTFWSNQAAQYTGKPHRAVFEDSLGKIYRNLFKKASSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT19</accession>
    <name>W5MT19_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Somatostatin 1, tandem duplicate 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Somatostatin inhibits the release of somatotropin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the somatostatin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638036.1">
        <property type="nucleotide sequence ID" value="XM_006637973.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MT19"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011528"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011545.1">
        <property type="protein sequence ID" value="ENSLOCP00000011528.1"/>
        <property type="gene ID" value="ENSLOCG00000009445.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696005"/>
    <dbReference type="KEGG" id="loc:102696005"/>
    <dbReference type="eggNOG" id="ENOG502S79W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047914"/>
    <dbReference type="HOGENOM" id="CLU_124515_1_1_1"/>
    <dbReference type="InParanoid" id="W5MT19"/>
    <dbReference type="OMA" id="QCYLALL"/>
    <dbReference type="OrthoDB" id="9948948at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009445">
        <property type="expression patterns" value="Expressed in embryo and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004250">
        <property type="entry name" value="Somatostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018142">
        <property type="entry name" value="Somatostatin/Cortistatin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558:SF2">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03002">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001814">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866520" evidence="9">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Somatostatin/Cortistatin C-terminal" evidence="10">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001814-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="116" mass="13184" checksum="6601F2B41DAD8938" modified="2014-04-16" version="1">MLSYRVHCTLALFGLVLAFCCVSAVSHSDLRFRRFLQKAKAAGLNKQDSSKYAIEDLISQLVQSDSEALENEMSVLDENSDARLELERSTEIGKEIPPRERKAGCRNFYWKTFTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVY3</accession>
    <name>W5MVY3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CD99 molecule (Xg blood group)</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD99 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217208.1">
        <property type="nucleotide sequence ID" value="XM_015361722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVY3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012542"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012565.1">
        <property type="protein sequence ID" value="ENSLOCP00000012542.1"/>
        <property type="gene ID" value="ENSLOCG00000010250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687711"/>
    <dbReference type="KEGG" id="loc:102687711"/>
    <dbReference type="eggNOG" id="ENOG502S70S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154344"/>
    <dbReference type="HOGENOM" id="CLU_092825_1_1_1"/>
    <dbReference type="InParanoid" id="W5MVY3"/>
    <dbReference type="OMA" id="DNQIFSN"/>
    <dbReference type="OrthoDB" id="8963727at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010250">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000391">
        <property type="term" value="P:positive regulation of neutrophil extravasation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072683">
        <property type="term" value="P:T cell extravasation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022078">
        <property type="entry name" value="CD99L2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076:SF15">
        <property type="entry name" value="CD99 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076">
        <property type="entry name" value="CD99/MIC2 PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12301">
        <property type="entry name" value="CD99L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868871" evidence="8">
        <location>
            <begin position="19"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="216" mass="22115" checksum="D0D14596B4DA18C0" modified="2014-04-16" version="1">MKYLRILLFAILTIVAQGQDGFDLSDALGGSDPVTAKPKPKPPKDTGTGDFNLGDVFDMPGPTKPPVTPKPKKPSGDEFDLGDALGGPDPHPTKQPAVPPKNPGKGGSFDNDDLGYVAGEGDYNPDKGKSGGRSGGRAEGDTQGGSDQPQAEGSGKIAGIVSAVGVALLGAASSYFAYQKKKLCFKISGGDPESGNKTQSGTQSEPQILSNLLRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXG0</accession>
    <name>W5MXG0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXG0"/>
    <dbReference type="FunCoup" id="W5MXG0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013069"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013097.1">
        <property type="protein sequence ID" value="ENSLOCP00000013069.1"/>
        <property type="gene ID" value="ENSLOCG00000010667.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162679"/>
    <dbReference type="HOGENOM" id="CLU_062642_1_0_1"/>
    <dbReference type="InParanoid" id="W5MXG0"/>
    <dbReference type="OMA" id="PQRDEIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010667">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20526">
        <property type="entry name" value="CYCLIN_CCNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000006">
        <property type="entry name" value="Cyclin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000052">
        <property type="entry name" value="cyclin-I isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="52"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="40971" checksum="5793A8B89504FBDF" modified="2014-04-16" version="1">MKCPTGSDECRLAILLEDALSRESRLWKAPVFKHSRSQGTDISPSQYEEVVLWLLELGTFFNYCPETFALAVCILNRMLASVKAQPKYLRCIAVTSLILAAKTNEEDEVIVSVKDLAVQSRCKCSPAEILRMERTILNKLHWDLYIATPVDFLNIFHAMALSSHPRLPGVLAQKIPSLQAELWTRQVQHCMACHQLSQFKGSTLALATITLELERLTPDWFSVFTDLLKKAQIDSTELIRCKELVDQYLSGLQFSLPPNTVYIFHPANRDALYCERSIPELCPAVLRKRATQPSLPGRVLCQFRKAEQHNGEVEADEFYDGFRCLYNEGIAVEVGGNDTVNPHEALQENGSPCPPLQPPVRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYE0</accession>
    <name>W5MYE0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01003653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYE0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013399"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013428.1">
        <property type="protein sequence ID" value="ENSLOCP00000013399.1"/>
        <property type="gene ID" value="ENSLOCG00000010924.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159161"/>
    <dbReference type="HOGENOM" id="CLU_004885_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYE0"/>
    <dbReference type="OMA" id="FNNHQQP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010924">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003139">
        <property type="term" value="P:secondary heart field specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21777">
        <property type="entry name" value="MobB_LATS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14398">
        <property type="entry name" value="UBA_LATS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001246">
        <property type="entry name" value="Large tumor suppressor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000199">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000029">
        <property type="entry name" value="Serine/threonine-protein kinase LATS1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF149">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LATS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="100"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="525"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="379"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="861"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="467"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="871"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="939" mass="103566" checksum="95EA7F48BCC65D7C" modified="2014-04-16" version="1">RRGAGGRAGAAMRPKTFPATPYAGNTRQRLQEIKEGLKQPAKLVSQALQGGRGAEGGRPPPQQHHPRAPQKFGNYQTALREIRRSLLPFANDSGSSPGPEVNRQMLQELVNAGCDQEMAVRALKQTGSRSIEAALEFISKMGYLDPRTEQIVRVIKQTSPGEGAFGGGLECCFFARAACDQTRVSSEPRPGVRQTPPSGRWESGVSFLFVLGGSVLEREQRGTSRVSFRAVAPGGYRAHRDGSYCVVVGKGPSRASLNLDLYEHHWAGEGAARQPPPPGPYRGEVHAPSRTNSFNSPQKVSVAVRPEPPLGPPSTITAVTSAPVQQPVKSIPVMWPEPRTAVGPCHPGWLAAQAPEAAEACPYAAGEAYAPGPRCPPPPYPKTLLLHGAPEPPEGKGPSGQAEEGSSGALAGDKAKTGRAEKSVKDKKQIQTSPVPVRKNARDEEKRESRIKSYSPFAFKFYMEQHVENVMKTYQQKLNRRMQLEQEMAKAGLSEAEQEQMRKMLYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLTRKVDTNALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDNLYFVMDYIPGGDMMSLLIRLGVFPEPLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHIRQDSMEPSDLWDELSGCRCGDRLLTLEQRAARQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQMKVINWTSTLQIPPQAKLSPEAADIIGRLCCAAEERLGGNGASEIKAHPFLEQMDFSSSLRTQPAPYLPRVRHPMDTSNFFDPVEEGGAGARGDSAGSARAGDAPGSPHSKHPEHAFYEFTFRRFFDDNGCPFRYPKPADGLPGGAGAEEQAEGCEPVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ29</accession>
    <name>W5MZ29_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Missing oocyte, meiosis regulator, homolog (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat mio family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ29"/>
    <dbReference type="FunCoup" id="W5MZ29">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013638"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013667.1">
        <property type="protein sequence ID" value="ENSLOCP00000013638.1"/>
        <property type="gene ID" value="ENSLOCG00000011101.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015038"/>
    <dbReference type="HOGENOM" id="CLU_005843_1_0_1"/>
    <dbReference type="InParanoid" id="W5MZ29"/>
    <dbReference type="OMA" id="YWIASYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011101">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060841">
        <property type="term" value="P:venous blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000103">
        <property type="entry name" value="Meiosis regulator for oocyte development"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21720">
        <property type="entry name" value="MIOS_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="11">
        <location>
            <begin position="395"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="10">
        <location>
            <begin position="741"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="877" mass="98219" checksum="1B3D3D02359CB31E" modified="2014-04-16" version="1">MSASKPDILWSPHHVDRYVICDSELSLYRIASLGSAETKAGVLQLSGETAATLLAINSDTPYMKCVAWYPRPEPECLLAVGQANGRVVLTSLGQSHNTKCKELMGKEFVPKHARQCNTLAWNPVDSNWLAAGLDKHRADFSVLIWDISSKFAPEASVPTEKLRLSAGDTDAGLVVTKPLYELGQNDACLSLCWLPRDQKLLLAGMHRNLAIFDLRNTSQKMFVNTKAIQGVTVDPHFHDRVASFFEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNVIRLYDMQHTPTPLGDETEPTIIERSVQPCENVIGSFAWHPSSQNRMVVVSPNRCMTDFTVFERISLAWSSSTSLMWACGRHLYDCTEEAEAGSLEMDIATKMRQRAQSRYGHDTEQIWRNHLLAGGDDPQLKSLWYTLYHKPLMKQYTEDLEQKNLGNRLPLVYSGIKNIVKSSSGTLESCRCWNGSDRPADVTQYRSEERSLALRLCGWIKRGPEIDVEPFLKSLEQEGEWERAAAVALFNLDIRRAIQILNTGASSGKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLSNPYLCVMFAFLTSEPGAYDGVLYESRVAVRDRVAFACMFLSDGQLPRFIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRTGDVQTASFCMLKGSPEEVVKDRRVQCWIESYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAMPHQGRGFNQYGVSSSPTKSKVTSCPGCRKPLPRCALCLMNMGTPVSSCPGTGKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSWFRDHHECPVSACTCKCMQLDTTGNLAPSDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZU0</accession>
    <name>W5MZU0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZU0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013899"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013928.1">
        <property type="protein sequence ID" value="ENSLOCP00000013899.1"/>
        <property type="gene ID" value="ENSLOCG00000011310.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155025"/>
    <dbReference type="HOGENOM" id="CLU_1165501_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZU0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011310">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000021">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF97">
        <property type="entry name" value="GALECTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="96"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="25859" checksum="82AF4991824CD98D" modified="2014-04-16" version="1">MGGPGLLVLWAGGRPSAKRLKPNVRSTVVRCALIWGVVIRRELWSIGLRFTAGLVGTPPFLVIGGRQRGGTESSIRKEGLSTCGNLESPVEVEKPGETCFESRTGPSRLVFCSARPTSCRPKFAINVGSSSSDLALHFNPRFYDYTAGGQNSIVMNSAPGGSWGREQRESHFPFKRGEKFTMSITHNGNSFGIKLPDDRVISFPNRLGRDSYDHVSFYGDIQIFSIKAEVGGAGQASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0E3</accession>
    <name>W5N0E3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TBC1 domain family member 9B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0E3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014102"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014131.1">
        <property type="protein sequence ID" value="ENSLOCP00000014102.1"/>
        <property type="gene ID" value="ENSLOCG00000011473.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699220"/>
    <dbReference type="KEGG" id="loc:102699220"/>
    <dbReference type="eggNOG" id="KOG4347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158554"/>
    <dbReference type="HOGENOM" id="CLU_003535_0_1_1"/>
    <dbReference type="InParanoid" id="W5N0E3"/>
    <dbReference type="OMA" id="CTGEVPT"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011473">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13351">
        <property type="entry name" value="PH-GRAM1_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13354">
        <property type="entry name" value="PH-GRAM2_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000013">
        <property type="entry name" value="Putative TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000041">
        <property type="entry name" value="TBC1 domain family member 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000002">
        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000130">
        <property type="entry name" value="TBC1 domain family member 9B isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
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    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="Rab-GAP-TBC_dom"/>
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    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
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        <property type="entry name" value="TCB1D9/TCB1D9B_PH-GRAM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036017">
        <property type="entry name" value="TCB1D9/TCB1D9B_PH-GRAM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF5">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 9B"/>
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        <property type="entry name" value="EFh"/>
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        <property type="entry name" value="GRAM"/>
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    </dbReference>
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        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
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        <property type="entry name" value="EF_HAND_1"/>
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        <property type="entry name" value="EF_HAND_2"/>
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        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="13">
        <location>
            <begin position="497"/>
            <end position="684"/>
        </location>
    </feature>
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        <location>
            <begin position="868"/>
            <end position="903"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="965"/>
            <end position="984"/>
        </location>
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        <location>
            <begin position="1059"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1219" mass="137887" checksum="E4296EA7004640E6" modified="2014-04-16" version="1">MWIVPEEVLLANALWVSERANPFFILQRRKGHGKGGGFTGLLVGTLDVVLDSSARVAPYRILHQTQDSQIFWSIACGSSRKEITEHWEWLEANLLQTLSIFENEEDITTFVKGKIQGIIAEENKSKETTEEEDSGKFREAELKMRKLFGMPDEEKLVNYYSCSYWKGRVPRQGWLYLSVNHLCFYSFLLGKEVTLVVQWTDVTQLEKNATLLFPESIRVSTRDSEHYFSMFLNINETFKLMEQLANIAMRQLLDNESFSADRSLPRPCKPLKNVSALKRDLDARAKNERYRATFRLTQDEKLDGHTDCTLWTPFSKMHVAGQMYVSNNYICFSSKEEDFCQLIIPLREVTIVEKADSSSVLPSPLSISTKSKMTFLFANLKDQDFLVQRISDFLQRTPEKLWHNGKEQSDPPSPGFPLSASPPSPGSSQPGRFYSPDLPTAAQGLLSIFRRDAPEDLGPKATKEKMKEELWNIHFFEYGRGVCMYRTSKTRDLVLKGIPDSLRGELWLLFSGAQNEMATHPGYYAELVEQSMGRCTLATEEIERDLHRSMPEHHAFQNEMGIAALRRVLTAYACRNPNIGYCQAMNIVTSVLLLYCSEEQAFWLLVALCERMLPDYYNTRVVGALVDQGVFEELTRECLPLLYERMQELGVISTISLSWFLTLFLSVMPFESAVLVVDCFFYEGIKLIFQVSLAVLHANMEQLLACSDEGEAMTILGRYLDNVVNKQTPSPPIPHLHALLTSGDEPPPEIDVFDLIKSSYEKFGTLRADVIEQMRFRQRLKVIQSLEDTAKRSVVRAIMTESAFGIDELEELYVLFKAKHIMSCYWGASSSAAERHDPSLPYLEQYRIDMGQFSQLFGALTPWSCGVHTLALAARLFRLLDQNKDGLINFKEFVTGLSGMYHGDMTEKLKMLFKLHLPPALCPEEAESALEATQFFTDDASPQDSPFLSEMDFLLQDVISGENAKPIATEEGTSNGNSENKKEEKPQDYKYYLRMWAKEREPKKETIKDLPKMNQEQFIELCKTLYNMFSEDSCEQELYHSIATVASLLLQIGEVGKKFSSNGGKKPEHNDVAGPVSDMPEEERPAESPGGNPKEAQVSQAAEEPAEVPQLPASDEDTKDDTSVSSYSMVSAGSLQCEDISDDTVLIGCGDQRRGSSLDGDWSISFEQILASILTESPLVDYFEKKVDIRAKMAACKAQRTVERQVSSSSECELPLLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0G1</accession>
    <name>W5N0G1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Proteinase-activated receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Coagulation factor II receptor-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Thrombin receptor-like 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0G1"/>
    <dbReference type="FunCoup" id="W5N0G1">
        <property type="interactions" value="247"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014120"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014149.1">
        <property type="protein sequence ID" value="ENSLOCP00000014120.1"/>
        <property type="gene ID" value="ENSLOCG00000011493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691805"/>
    <dbReference type="KEGG" id="loc:102691805"/>
    <dbReference type="eggNOG" id="ENOG502QU4Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244840"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5N0G1"/>
    <dbReference type="OMA" id="WGNLYGA"/>
    <dbReference type="OrthoDB" id="8716763at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011493">
        <property type="expression patterns" value="Expressed in heart and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15372">
        <property type="entry name" value="7tmA_PAR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000230">
        <property type="entry name" value="F2R-like thrombin or trypsin receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF22">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 19" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="13 19" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866976" description="Proteinase-activated receptor 4" evidence="21">
        <location>
            <begin position="25"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="231"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="81"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="136"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00053164"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="44738" checksum="8606D265FD8700FB" modified="2014-04-16" version="1">MDSPSFRQPFSVVLLFITFHTFTCEEKECVSTLQIVRSFNIHSKCNRTVLDEKDQAHVKGPVTTLLIPLLYLLALVVGLPANVTAMWVLLAKTKKMPSTILLINLTTADLLLILVLPFRIAYHFQGNNWIWGESFCRTTTAMFYGNMYGSILCLMLISIDRYIALVHPFGAKVFRSRKTSIYMSLVIWVIVIGAMLPLLLTQQSYTLNEPNITLCHDALPQKEQETFFFPYFASLFTLAFVIPFCVILFCYCSIIWTLMRSQERYTHAAKITALVLVVFIACFLPSNMILLIHYSESHLIVGKQDLYVPYLFSLALSTFNSFIDPFIYYYVSEDFRNKAIKLFVCCRTKNYFISTLSSRNQDSYSASKFRSVSTEMSALNGGQLQKNQCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0L7</accession>
    <name>W5N0L7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014176"/>
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        <property type="protein sequence ID" value="ENSLOCP00000014176.1"/>
        <property type="gene ID" value="ENSLOCG00000011534.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692407"/>
    <dbReference type="KEGG" id="loc:102692407"/>
    <dbReference type="CTD" id="64411"/>
    <dbReference type="eggNOG" id="KOG1117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158869"/>
    <dbReference type="HOGENOM" id="CLU_002900_1_0_1"/>
    <dbReference type="InParanoid" id="W5N0L7"/>
    <dbReference type="OMA" id="TLNTMEM"/>
    <dbReference type="OrthoDB" id="29546at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011534">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13253">
        <property type="entry name" value="PH1_ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13259">
        <property type="entry name" value="PH5_ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17228">
        <property type="entry name" value="RA_ARAP3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RhoGAP_ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000170">
        <property type="entry name" value="Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf-Rho-GAP_ANK-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037858">
        <property type="entry name" value="RhoGAP_ARAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899:SF4">
        <property type="entry name" value="ARF-GAP WITH RHO-GAP DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="468"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="575"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="661"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="881"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="994"/>
            <end position="1092"/>
        </location>
    </feature>
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        <location>
            <begin position="1096"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="11">
        <location>
            <begin position="1306"/>
            <end position="1400"/>
        </location>
    </feature>
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        <location>
            <begin position="1413"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1669"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1739" mass="197290" checksum="5D4F28492222B943" modified="2014-04-16" version="1">MATPCGDCSDIGKLLASIHLEKYMDSFRQFGFQKANDFYHLDNETLIQIGVTATGHRKRILKLAEQIQQTARLQSKESTLDRSWSESAINQALPQDLGPDSPSESFSNTSELIRNKSVPNIPTIIEDSKVSEDVLVKPIPKPRTVFSRPKTEQQASCPKSEPQERNLSKESSQDCSLNCIVLLQGFSPGESNTDAEGLGLEQRECVRTPERRLSHSNSLESLAKTPGNLPPVPPRMHRGIPPASFTSPGSPGRAESNQLISPLSSSPVSPCSHDEESFSIVPMLSPPSSPHSSIEMVSNDIYCGTLPGTPSNACEGLRNRPVPLPPSRQDLSPSPEKNSTLKTSFVEPNKRGLVLQEEVISPYCETVLQSKRPNQEVKFARTQSEGTTGLLKKEEDGKEEDAGRRVLARDQRISEIMHSDSMGYATVGELHVPTRSFSLPPDMYGDELNEDLTISPYASFTSLSDHVAPVISSWLDKLSPQGNYVFQKRFVKFDGKNLMYFSSEKDVYPKGVIPLAAIQMARTAKDNKFEIVTSQRTFVFRAENEGLRYDWCRTLQNRVKDQQVTSRHRFGPGSHCQKSGYLELKGTKSKIYVAINTDQVWLHKNEQCFKNGIGITLIDVRGSTIRDNKSKTFELITPHKIFSFTAECEREKKEWMEALQESIAETLSDYEVAEKIWSNRYNKICADCKAVNPDWASINLCVVICKNCAGQHRGLGTTVSKVQSLKLDTSVWSNEIVQLFIMLGNDKANEFWAARLPPAEELDCDATPEQRREFITLKYREGKYRRMHPSFSTQEELLKALCSAVAEQNLLKTVLYIFSEAEAAHLSDTNGYDKCHSPHHKSTFDSHSHTTSDFSFSDGSQSVYDEIMQPVMYSGYIYKSSALTKGTLSRKTREDFQKYWCSVERSLLFYESDRNHDPSMKIDVKDIICLGVSRPDSSNSTGFIDRFRYTFELYLSSEKLYQFGLETAEGLHSWASAIGKAFTPLSCHCLLAREFERIGRLRYKAMLDLEQWKEGYFVLQKSNLFICPGNDGAAEDIVNLKRLQELSITSQNDNNEKKDILVLVEKGRTLYLQGTGRMDFSLWYTDIQTAAGGKGNTLRDQQLSRNDIPIIVDSCIAFITQYGLRHEGIYRKNGAKSRIKLLMEEFRKDARNVKLRIGDNFIEDVTDVLKRFFREIDDPVFMADLHPMWKEAAGISNKMRRLDRYKDLIRSLPRVNRTTLAALIGHLYRVQKCADLNQMCTKNLSLLFAPSLFQTDGKGEHEVKIIEDLIDNYLYVFDIDDEQQTQIELEISLITTWKDVQLSQAGDLIIEVYLEKKLPDCCITLKVSPTMCAEELTNQVLYMRNVPAGEKDVWLTFEAIEDEELERPLHPKEKVLEQALQWCKMPNPSSAYLVVKKVPRTEAINIFTAYKCDIVKSGVLKCREEHPKMLQGNRFQERSFQIRDHKLVLLKDKKSNKPEKEWPLKTLKIYIGIRKKLKAPTQWGFTVLLDRQQLYLCCSGEAEMWDWMVSFLKAQHDDLRPPVLRRHSSSDISKQKFGTMPLVPIRGDESNSTMFSANQTLRKLHARRTLSMFFPMKMQQDSFEEQSESPEPLYEEVGDFGMQVLKSLETSFLSSTDAETQEVRGVNSAKTLDRPTSLGQRKTASLDRMIGPNPVKVLMTESPRGLTPAASLDTLRSGQPVFLDDTGQPDSKMTRPNPRKKIIGHGSPTQDRLLEELSNVFVKKNETDSPTKHDEDNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0X0</accession>
    <name>W5N0X0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DEF6 guanine nucleotide exchange factor a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0X0"/>
    <dbReference type="FunCoup" id="W5N0X0">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014279"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014308.1">
        <property type="protein sequence ID" value="ENSLOCP00000014279.1"/>
        <property type="gene ID" value="ENSLOCG00000011619.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUWV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183017"/>
    <dbReference type="HOGENOM" id="CLU_029358_1_0_1"/>
    <dbReference type="InParanoid" id="W5N0X0"/>
    <dbReference type="OMA" id="FEHYRYY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011619">
        <property type="expression patterns" value="Expressed in mesonephros and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13273">
        <property type="entry name" value="PH_SWAP-70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057836">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057837">
        <property type="entry name" value="PH_SWAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383:SF2">
        <property type="entry name" value="DIFFERENTIALLY EXPRESSED IN FDCP 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383">
        <property type="entry name" value="SWAP-70 RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25530">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="213"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="550"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="406"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="583"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="71610" checksum="D2D1413CA0F1B7D1" modified="2014-04-16" version="1">MELRGELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLCTVLSIPHDPVALEEHFRDDDDGPVSSQGYMPYLNKYILDKVEEGAFVKENFDELCWTLTAKKNYRPEKNGKSVLSMHDAFRLWCLFNFLSEDRYPLVMVPDEVEYLLKKVCVAMSMELNCVELEDYISQEAVQQSGITVWHFLDLVNSGKITRGIDKQTISMAIEEVYREIVGDVLKEGYLWKRGQLRRNWTERWFTLRPNSLAYFVSEDRKERKGSISLDRNCCVEVLPDKDGKRCMFCVKTPAKTYEMSASDTKQRQEWTTGEKTHIIHTNASQPKCLHIHALCVHEGLTREQGRREAKSNEVQQIHRFHYKSRTETSDAQFLFTSQRHAGQVLLIYGFVKISPFVCEWSEAVRGTTRCAVSQARASMQAEMALKEAEADRQRKRIRELEEMQQRLEEALHLEIKARQDEEAFRYAQARLLAEEEEKLKALMALKEEQEEYILKTQREKQELKQEMESKSRSLDEAQRQLEEVRANRHRVDQDVVAAQRKLRQASTNVKHWNVQMNRLMHPIGPGEKRPSGSFSSFRVPMQKDPGLKLQLRQRSEEKDEESKENVNRGEGAKRLSQASDSDMDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0Z8</accession>
    <name>W5N0Z8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein mL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">39S ribosomal protein L38, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phosphatidylethanolamine-binding protein family. Mitochondrion-specific ribosomal protein mL38 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0Z8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014307"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014336.1">
        <property type="protein sequence ID" value="ENSLOCP00000014307.1"/>
        <property type="gene ID" value="ENSLOCG00000011636.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141125"/>
    <dbReference type="InParanoid" id="W5N0Z8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011636">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000002">
        <property type="entry name" value="39S ribosomal protein L38, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF133">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="384" mass="44786" checksum="26819AED2D3B9285" modified="2014-04-16" version="1">MAARMVCGVVLRTGTDLGVFRRFGTSATACRRAAPLGPMPNEDIDARNLESLEKYRSYTRYLKVAEEESRKPHWWKTYRQYVVQEEEGAEKIDIGLPRLQLSRSQEVKERRKLCRENRRSSELERATRLQTLRIPLDEVKAEWERTNGPYDVQRVAEYYGIYRDLFPHATFVPRVVLRVVYGKEGEHSSPVHCGNVLTPSEAASAPHVTFGAEDGSLWTLLLTSPDEHLRDNESEYVHWLVGNIPGNMVHSGEEVCHYFPPFPAKGTGFHRFVFILFKQEGPINFKEDYRQSPCHSLTMRTFRTLDFYRKHQDAMTPAGLAFFQCQWDDSVTHTFHEMLSMKEPVFEYDRPPVYHPPQKKYPHGQPLRYLDRYRDSQEPSYGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N148</accession>
    <name>W5N148_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuritin 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N148"/>
    <dbReference type="FunCoup" id="W5N148">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014357"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014386.1">
        <property type="protein sequence ID" value="ENSLOCP00000014357.1"/>
        <property type="gene ID" value="ENSLOCG00000011681.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689326"/>
    <dbReference type="KEGG" id="loc:102689326"/>
    <dbReference type="CTD" id="449839"/>
    <dbReference type="eggNOG" id="ENOG502RZNR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063853"/>
    <dbReference type="HOGENOM" id="CLU_135101_0_0_1"/>
    <dbReference type="InParanoid" id="W5N148"/>
    <dbReference type="OMA" id="CAYWEDF"/>
    <dbReference type="OrthoDB" id="9928047at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011681">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF1">
        <property type="entry name" value="NEURITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867101" evidence="10">
        <location>
            <begin position="28"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="142" mass="15764" checksum="5313B6813A309029" modified="2014-04-16" version="1">MGFTLNGRYIALILAVQLVYLLQAVKAAGKCDTVFKGFSDCLLRLGESMVHYPQDLDEKENLKTICSYWDDFHSCATTALADCQEGATDLWEKLKKQSKNLEFRGSLFELCGGSNGAPTSTIHFGFTVFLISLSALVTWFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1N1</accession>
    <name>W5N1N1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein RRP5 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Programmed cell death protein 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for the generation of mature 18S rRNA, specifically necessary for cleavages at sites A0, 1 and 2 of the 47S precursor. Directly interacts with U3 snoRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with NF-kappa-B p50/NFKB1 and NF-kappa-B p65/RELA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014540"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014569.1">
        <property type="protein sequence ID" value="ENSLOCP00000014540.1"/>
        <property type="gene ID" value="ENSLOCG00000011818.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012228"/>
    <dbReference type="HOGENOM" id="CLU_000845_1_1_1"/>
    <dbReference type="InParanoid" id="W5N1N1"/>
    <dbReference type="OMA" id="GQYLRAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011818">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05694">
        <property type="entry name" value="S1_Rrp5_repeat_hs2_sc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05695">
        <property type="entry name" value="S1_Rrp5_repeat_hs3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05696">
        <property type="entry name" value="S1_Rrp5_repeat_hs4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05697">
        <property type="entry name" value="S1_Rrp5_repeat_hs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04461">
        <property type="entry name" value="S1_Rrp5_repeat_hs8_sc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000175">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000200">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000148">
        <property type="entry name" value="protein RRP5 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000340">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.162, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="85"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="189"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="280"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="452"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="541"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="630"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="723"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="1020"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="1210"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="1304"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="1652"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1375"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1487"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1396"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1424"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1798" mass="199513" checksum="61AF600084DC882F" modified="2014-04-16" version="1">MASVEEDFPRGGKVKRAEGSQPVRNHVEQDNLFEARDPEEGKKRKKKRTEEEKTSKKRMNREEETLKLNASKNVEILHLRNVTVGTLLLGCVKEVNDFELVVSLPSGLTGFVQITNISDSYTKLLSEQLDSAQSLEETLTLPRLFPPGMLLRCVVSSVEVTKGGHQSLKLSVNPREVNKALGAGSLKAGMLISGSVESIEDHGYLVDIGVSGAKAFLPRQKAKDLQLESKGLMVGQYLNCLLEEVKNEGRIVRLSVGPSEIAKAVADTQQGWTLNSLLPGLIVKAKVKKVTIHGPILEFLSSFTGTVDFLHIDSEKASDYTPGDTVKACVLYIDPSTRTVGLSLRPHLLHPGSKLHTVSNTQIGEVVQDCKVKSFHQHSGAILKLPDGSLVFAHKNHLKESHESFDPNRTLAGSTHTCRILDFSPLEQMLLVSLRKSVIEAPFLQYQDLQPGQLVTGIVTSLHDFGMLVKVTEHIRGMVPRTHLADIILKNPEKKYRPGDEVKCRVLTVDPKVKNLVLTRKKTLVESTLPLICTYQDAKPGQMSHCYIVSVKDFGCIVRFYGNVKGLVPVGELSMEPKVIPEKLFYVGQVVKAKVMKCDPHQEKLLLSFKGVAEDIQTVPEESSFEYEVGKTVEARIVQKTDSGLKVSILPEEAAAFLPTVHLSDHVSNCSLLWEGLREGDIISNVVCLSKSKEQIILSKKPSLKAVFEDGVVARNFAELNAGMLLAGWIKNVMSYGVFVEFPYGLVGLAPISAMSDKFVTNTGDAFKVGQTVVAKVTNLDEEKRRFLVTLKVSEVSLEREEAAALLSQCIQERNTVAQMMANQGHSELLQSLSSLKVGQKLKLTVDEVNDNGTATFKADHLNGVTILASKYHLTGVTVVEGQKVTGVILHVDFLTRQLHISLSPLLVSSKCKLKANVTMPATLQYVERDFAVIRLGETGHLTFISTKTHWNDTFRFESERLSVGQCLTVTVKKPKSEELGGFPSVTWVSGKIVGSRRERSLSQSKDEKLGGPKHPFSLGDTVKGTIKTIRPLSVLVALEGGITGSIHVSEVLEQVKPGTFPVSTLTKGSTVTARVIGGREVRSHKFLPISHPNFTFTIPELTLLPSKMEGDLPTQEEHAEALENYQPGQEVTCFVYKYNTVKKCLEVDVTPVLGGKVDLLLMSLTPKELKHPEKLCKLGQALKATVVSTSSCKTKLVLSLTGLHSLTNGSITLGTVAKIVKRVGLVVSLPFGHTGRVSVLDLDDCYRPNPLDSYHVGQVVRCCIVESEENKLQLSLRRSRTTPGSSQVVKDPEIPSIADLKEGQRLRGYVTSIKDCGVFISLSRSIIGRAQFHHVTKHFTADHSVFVKHIPLNSLLTTKVLSIDKEKGQVELSLLPEDTGGPDVLPESLGLPLRMKVEETTEDSQKKRKRQTSESEQEAIEKSKKKKKKKTSGKSEENDSGVEVYFREDEEENKKLPSKKKAQPAQTECVSPQRLHVSSGFSWDTGLSTLRPATVDPVADSSSEEEEENTEKKASHPQNSILPFNRQPIHLSKEVLILLMEPNSSLVWLQYMAFHLQATEIEQARAVAERALKTISFREEQEKLNVWVAFLNLENMYGTEESLQKVFKRAVQYCEPMAVFQQLADIYTKSNKHKEADSLYNTMLKRFRQEKSVWLSYGTFLLKQGGNDAAHRLLQRALRSLPNKEHVDVIAKFAQLEFQYGDVERAKAMFDSTLSSYPKRTDLWSVYIDLLIKHGSQKEVRNVFERVIHLSLAAKRIKFFFKRYLEYEKKHGSAETVEAVKAKALEYVEARGSVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1Z9</accession>
    <name>W5N1Z9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ninein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014658"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014687.1">
        <property type="protein sequence ID" value="ENSLOCP00000014658.1"/>
        <property type="gene ID" value="ENSLOCG00000011900.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QZCC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000095541"/>
    <dbReference type="HOGENOM" id="CLU_001462_1_1_1"/>
    <dbReference type="InParanoid" id="W5N1Z9"/>
    <dbReference type="OMA" id="QKVELLX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011900">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090222">
        <property type="term" value="P:centrosome-templated microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905">
        <property type="entry name" value="NINEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905:SF11">
        <property type="entry name" value="NINEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="251"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="685"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="431"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="949"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1033"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1142"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1211"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1341"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1512"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1612"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1869"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1992"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="691"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2099" mass="242987" checksum="D82ACC641D590F05" modified="2014-04-16" version="1">MDEAQQDQYEAQLKEVFDSFDTSGSGSLGQEELSELCRALHLEKVAPTLLHTLLPEEGLHTDRVHFEQFKDALILVLSATLGGPLSNPVDHPEPVCNSAEVVPKFVKDGKRYGRRSTPEGRDPTAGSCEVIGTVQALNEPEDSPNTSVQNDSEHCSTSVHNSEEYEAEGQLHFWNPDDLGTPRGFAPSPDELEECLQGACEELGLCRDGFASHQELLSVCARLGLEVVEDVFQKLDSDGVMRVQDFVSGIVRSNNPPTPSASTPYRQLKRHHSTQPFDESGRRTTTPSAMTSTIGLRLFSTLDDGSGYASAEQLLDTWQEEGIENGPEILQALDFGLERKVNLSELTIALENELLITKNGIHQAVLASFKAEIRYLLECVDRELREKRKVQADLEKADKLKTQMATEVDEHHSAIERLNDYNLRKLEMEHRDKLAAVKSELTRELDHIQQQATRQRAQLEREVEKVKEEEAFLRDRLSLTLKENGRLEAELLENTEKLVESENLVGKLQKNLENILKEKFGDLDPGSAEFFLQEERLRHLRSKYEEQCRELQDRIDELQEELEEYRTLGRTSRPFFKPSLSEEFDSKSPGIESDPGLGSEDCPPFNMSLEAEMLIEQLKDQHVRDLETLRGELDGKVSQYEQQLEELKGAHEQQRRELAQRCQEEKDQVQRRAEELQNLLDAAQTAQEGLEQSHAEERAELERRHAEEVAERDRLHSEDRVRLEQQHEAALQGRLEEQRQRLSGALEERERQLAEEWERQRSLMEESHREAVQELSARHSEERERLNGLVEKLKQDFLEERKELERHYSQKIRELESRFSDQEAVSERLQKDMSKLELRHQNELQDLTMRHTEEKAQWEFEKEEILRESEEEMERLRDSLKREKECVSQEFEREREMMEKSYKEQLNGLIAKNEQVQHELEAFIHTAKVKESELTKQLADLPKDLQQNLNAKDELLIQAQKKADDLELLLKQAVEDFEQEKAEIQGQLTDLEEKNKENLNLLEKQWAEIQSLVAENSRLQIRILELGTEVTKMSKLQERFEEVQKENEEALCMISHLKKRTEELEAKKESLTDLQTVKIRYPDEKTPTSESSIGTEPQVSEEKEQGCDSDSDLLKENIRKLEKPNEGIHQLQDEVEQAVKARKLCHSEMENLHEKLKQLQDQVYLHAGVQAQLENAHDRNLQLQQEVCHLEKRTSELEVLLEDDAQKIAAGEQSQKENKELRQDISRMIERTKDLEGKTKQLIDIQSRYEECTREKRKLQEEKMNLENKVHELEEKMQGVGHIQGRHSKLQKQVARMVEENSKLCAEIQELKKHEELKNLCQDTKGTKEVAEMNVHKKETFQDLNSQLEAKIQAVSELEDSCTELEREKAKLQETVAELQEKCQTLQEKMQAHRDKIKGVQCVMSVHACLGNTFTAQYSWKLSHRIYVVLECLFRDIILFLIEPSYEVVYTRSNPQFCTLSQTHLTKQLKSVSYPVCLQCRGEAGRLAEENLILKWEISKFKEEGNISQQELKDLNKSQEEMLRKIDQLKKEKTAALTMNESLKNQVLELRSRGQQLEDENGLLSHKSAQNAADVLELNRRLSELLKQREKKASSRRQHHQGELERERAALAAEFAQERSQMAARLSGLEKEMATAQERSAQLEQERALMSQELSTLKEQLCSSRDLRSELASAVSRSEKLQREKETLSEELSRCVDKVAKLGTMENQLSHLRQERQAAEQQAQNLKTQLSASLDKVVQALDEALQTVNLQTARLKSDLRVTQQEKEALKQEVMSLHKQLQNANNKNQFLELALHSSGYQSHQKKLYWEELSRLVDQEQQLLRQENERLQKEVQNTKGDLILSRDKTRQLESTILSLKQQKQQGQSSLVKAVEQEKLGVKRELDSVRKELLIANRKVSEQSESQREMESLRQENENLKARQARLETQLLELQQVRLTICHKKALQLQQLWACCFSGSKQFLQTSKHSCRSITRTKTKLDGQRSKRTSFITTKEGQEVVLLKMEERMRDVEQKLRNVKLLLQEKVTQLKDQLQKNAKADELIKDLYVENSQLLKALELTEQRQKVAEKKNYLLEEKISSLNKIVRDLSPSPLTAVPYHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2B2</accession>
    <name>W5N2B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SWI/SNF related BAF chromatin remodeling complex subunit D2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMARCD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023024">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2B2"/>
    <dbReference type="FunCoup" id="W5N2B2">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000014800.1">
        <property type="protein sequence ID" value="ENSLOCP00000014771.1"/>
        <property type="gene ID" value="ENSLOCG00000012004.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Linkage group LG15"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="SWIB_BAF60B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SWI/SNF-related matrix-associated regulator of chromatin subfamily D member 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMARCD2_SWIB_dom"/>
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        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
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        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="4">
        <location>
            <begin position="272"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="497" mass="57085" checksum="94A8526745724CF8" modified="2014-04-16" version="1">MTSRGVYPMNTGSPLNPNINPMNSGGVRIVPSMQQNSGFRAMGSAASPYHQRPGMPPSRGMPMGGMGVGPGAGPSYSSGLPMRAGLPQPAMDPFRKRLLQQQHQGLASHRRGIKRRKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIARKRMEIQEAIKKPITQKRKLRIYISNTYSPAKPEGDDAEKIASWELRVEGKLLEDPGKQKRKFSSFFKSLVIELDKELYGPDNHLVEWHRMPTTQETDGFQVKRPGDISVKCTLLLMLDHQPPQYKLDPRLARLLGVHTQTRASIMQALWLYIKTNKLQDCHEKEYINCNRYFRQIFGCSRMRFSEIPMKLAGLLQHPDPIIINHVISVDPNDQKKTACYDIDVEVDDPLKSQMSSFLSSTTNQQEIAALEMKIHETIESINQLKTQRDFMLSFSNNPQEFIQDWLKSQCRDYKLMTDTAGNPEEERRTEFYQAPWVQEAVGRYIFSKVQQFLAEFLQIWTLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3I9</accession>
    <name>W5N3I9_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206132.1">
        <property type="nucleotide sequence ID" value="XM_015350646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3I9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015198"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015227.1">
        <property type="protein sequence ID" value="ENSLOCP00000015198.1"/>
        <property type="gene ID" value="ENSLOCG00000012343.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684403"/>
    <dbReference type="KEGG" id="loc:102684403"/>
    <dbReference type="CTD" id="393674"/>
    <dbReference type="eggNOG" id="KOG2695">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164083"/>
    <dbReference type="HOGENOM" id="CLU_039586_1_0_1"/>
    <dbReference type="InParanoid" id="W5N3I9"/>
    <dbReference type="OMA" id="TRIWSFH"/>
    <dbReference type="OrthoDB" id="128867at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012343">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052254">
        <property type="entry name" value="CUL4-DDB1_E3_ligase_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472:SF1">
        <property type="entry name" value="DDB1 AND CUL4 ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23761">
        <property type="entry name" value="Beta-prop_DCAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="509" mass="57121" checksum="79A6692F716BD2D5" modified="2014-04-16" version="1">MKKGSWRDRGADRRRHHNPGWQRDHSSHRSQEERYGSSVPGTSRNVQAPPADSTSSSSRASAPDLPGFYFDPEKNRYFRLLPGHNNCNPLTREGLQKEQQESQRVRLLREDEQPRKKSPRVGLNSTLLFQKRHMGLVAESSYCRLIHELKVTSMRRHKLEVQSSDSTSPNTDNFKLIVADSACERVFTVNDVEHGGCKYGIMNFRGCSKGSLSVEMCDNLYFTNRKVNSICWASVTHSDSHVLLCLVGIAQTPGCVSLLPASLFSNSNPDQPGMLCSFKISTAWSCAWCLNPQADKNFSTGLSRRVIVTDAVTGRRQTYGISSDVLAQQFALRAPVLYNGCRSGEIFSIDLRQRARRGNSWKGGRFYQESAITSVRILHDENYLIAADMLGKIKLWDLRMSRAVQQYEGHFNEHAYLPLHVNEPERLLLAVGQDCYTRLWSLGDGRLLRSIPSPHPAGKDSVPSVVFSSQLGGRSGLPGLLMAVCQDLYYFSYNSDYQDGTLCPEGESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3P0</accession>
    <name>W5N3P0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferroptosis suppressor protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Apoptosis-inducing factor homologous mitochondrion-associated inducer of death</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">p53-responsive gene 3 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Putative FAD-dependent oxidoreductase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>menadione + NADH + H(+) = menadiol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:69695"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:69696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>menaquinone-4 + NADH + H(+) = menaquinol-4 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74079"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78277"/>
            <dbReference type="ChEBI" id="CHEBI:193091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:74080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>phylloquinone + NADH + H(+) = phylloquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74075"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18067"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:74076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ubiquinone-10 + NADH + H(+) = ubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:61984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:61985"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>6-hydroxy-FAD</name>
            <dbReference type="ChEBI" id="CHEBI:60470"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3P0"/>
    <dbReference type="FunCoup" id="W5N3P0">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015278.1">
        <property type="protein sequence ID" value="ENSLOCP00000015249.1"/>
        <property type="gene ID" value="ENSLOCG00000012383.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004582"/>
    <dbReference type="InParanoid" id="W5N3P0"/>
    <dbReference type="OMA" id="TWEIAPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008637">
        <property type="term" value="P:apoptotic mitochondrial changes"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0110076">
        <property type="term" value="P:negative regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
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        <property type="entry name" value="apoptosis-inducing factor 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="14">
        <location>
            <begin position="12"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037027"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040253"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042318"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048412"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="365" mass="39373" checksum="7AAD6BF0E578FE90" modified="2014-04-16" version="1">MGGQLSVDPSVHVVIVGGGFGALQPPGNCSPGVPFTLVDLRDAFHHNVSSLRASLQTAGFAPQTFIPYAKTFGECFWQGRMVQIDFEAQLREVLFSHLILCTGCDGPFPGKFNEVTIMGAAIGKYEDVVKQVSSSSVRVVVIGRRSTGIEMAAEIKTEYPHKKVVLIHSHVALADPELLPSVRQQAKEVLLDKGVQLVLVEKVSKLEALTNMTQKNTTVIRDKGTEITADLVIPCTGNKVNSTTYICLSGAAMVDNGALRVKAHLQVEGWENVYAVGDCADVKEPKMAYHAGLHAGVAVTNIIHSLTNKPLSCYKPGYMTMLAGRDGGVGQFSGIRLARTLVVQGKNMNLLLWKSCREMGQVALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4G6</accession>
    <name>W5N4G6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:100829</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5N4G6"/>
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        <property type="interactions" value="87"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000015554.1">
        <property type="protein sequence ID" value="ENSLOCP00000015525.1"/>
        <property type="gene ID" value="ENSLOCG00000012612.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVV5">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157357"/>
    <dbReference type="HOGENOM" id="CLU_077436_0_0_1"/>
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    <dbReference type="OMA" id="PQDPELC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012612">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="177"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="35075" checksum="ABFD4BC001ADC5D9" modified="2014-04-16" version="1">MLQQILKDMYIDPDVLEALNEEQKKILFLKMRQEQVRRWKEREESLEKEGASSVLGKPKPRKASTKNVSWLLGKDGDVHVCVIGESLGSTTSELIFSERGGRKVSGSFIRRHETGNLKSHLLNKMPSSEPFLKTKENIPAAQDDGIQLHFKGIPAHSNTAPGSLHSQEIPQDTKAYQAFSQHETNRDPQSSSIELEEENDSDTSPADPQDPELCYRPHPISRDSVKVRQEHVESQANNSDKSKDFKVIPAARKPVPESGREDGQAPPKGSFTRGRVAELAKSFNGLQVSSGKSSRAKPPVPTKPAHLQIASSSTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4R2</accession>
    <name>W5N4R2_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01002477">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4R2"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000015650.1">
        <property type="protein sequence ID" value="ENSLOCP00000015621.1"/>
        <property type="gene ID" value="ENSLOCG00000012698.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3MP">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064699"/>
    <dbReference type="HOGENOM" id="CLU_2533334_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4R2"/>
    <dbReference type="OMA" id="DFRNIEP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012698">
        <property type="expression patterns" value="Expressed in brain and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037643">
        <property type="entry name" value="HHLA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15299">
        <property type="entry name" value="HERV-H LTR-ASSOCIATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15299:SF3">
        <property type="entry name" value="HERV-H LTR-ASSOCIATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="84" mass="9619" checksum="0BC2E48F61928C40" modified="2014-04-16" version="1">SNYLFSMLSVTSHSSLALHKITILVYNISDFRNIEPSTFPMRYCYCFTNRTNDLTDFTAVLLDIMENSTSYLQELFKSSSILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5H7</accession>
    <name>W5N5H7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Placenta-specific protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023189">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5H7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015916.1">
        <property type="protein sequence ID" value="ENSLOCP00000015886.1"/>
        <property type="gene ID" value="ENSLOCG00000012904.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SEJ6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2580015_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5H7"/>
    <dbReference type="OMA" id="ACHEHRT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012904">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027941">
        <property type="entry name" value="PLAC9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15205">
        <property type="entry name" value="PLAC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8644" checksum="812A35A18768E10C" modified="2014-04-16" version="1">STGPGPGLLPRASHVSACHEHRTLHHKLNAVEQRVEDAVGKLEAEVSILLDAIEDPEWSSLLDTAGPVIDILDSKGEETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5S1</accession>
    <name>W5N5S1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Attractin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May play a role in melanocortin signaling pathways that regulate energy homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with MC4R.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023216">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023220">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630998.1">
        <property type="nucleotide sequence ID" value="XM_006630935.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N5S1">
        <property type="interactions" value="763"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015980"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016010.1">
        <property type="protein sequence ID" value="ENSLOCP00000015980.1"/>
        <property type="gene ID" value="ENSLOCG00000012964.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690801"/>
    <dbReference type="KEGG" id="loc:102690801"/>
    <dbReference type="CTD" id="569196"/>
    <dbReference type="eggNOG" id="KOG1388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155790"/>
    <dbReference type="HOGENOM" id="CLU_003930_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5S1"/>
    <dbReference type="OMA" id="CYXGTIS"/>
    <dbReference type="OrthoDB" id="9998912at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012964">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000860">
        <property type="entry name" value="Attractin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000072">
        <property type="entry name" value="Attractin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000079">
        <property type="entry name" value="Attractin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000022">
        <property type="entry name" value="Attractin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000008">
        <property type="entry name" value="Attractin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000013">
        <property type="entry name" value="Attractin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056732">
        <property type="entry name" value="GBD_ATRN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF2">
        <property type="entry name" value="DISTRACTED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24972">
        <property type="entry name" value="GBD_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="12 17" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1221"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="19">
        <location>
            <begin position="79"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="22">
        <location>
            <begin position="742"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="21">
        <location>
            <begin position="1005"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1022"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1034"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1371" mass="152283" checksum="821125E019256BF7" modified="2014-04-16" version="1">MGAENILRRGLFLFKWQNYPISGLRHINKYLVVYILFYLVLYTEAAFSKSCDKNCYSGRCFNGTCICDQGWVGDQCQHCQGRFKLTEPSGYLTDGPINYKYKTKCTWLIEGYPNAVLRLRFNHFATECSWDHMYVYDGDSIYAPLIAVFSGLIVPEVRGNETVPEVVTTSGYALLHFFSDAAYNLTGFNIFYSINSCPNNCSGHGKCTTGNSIASRVYCECDKYWKGEACDIPYCRNNCGSPDHGYCDLTGEKLCVCNDSWQGPDCSLTVPSTESYWVLPNAKPFSPSVGRASHKAVVHGKFMWVIGGYTFNYSSFQMVLNYNLESSIWNVVSINSGPLQRYGHSLALYQDDIYLYGGKIETSNGNVTDELWVFNIPNQMWSKKIPTVLVHGQQYGVEGHSAHVVEFDNGDVIMVIIFGYSSVYSYISNVQEYNLRTNTWLVPETKGAIAQGGYGHSSVYDSATKSVYVHGGYKALPANKYGLVDDLYRYEVRTRTWTILKESGFARYLHSAVIISGAMLVFGGNTHNDTSLSNGAKCFSADFLAYDIACDEWKVLPKPSLHRDVNRFGHSAVVSNGSMYIFGGFSSVLLNDILVYKPPNCEAFRAQEPCFSAGPGIRCLWIGDRCVPWDPFHMGDSNLAAQCPPKAIAADDRCYRYTDCASCTANTNGCQWCEDKKCISASSNCTMSVKNYTKCHVRNEQICSKLANCKSCSLNLNCQWDQQQQECHALPAHLCGDGWNHVGEACLRINSSRESYENAKHYCKNLSGNLASLTTAKEVDFVLDELQKYTVQKISPWVGLRKINISYWGWEDMSPFTNTTLQWLPGEPNDSGFCAFLERAEAEVVGLKANPCTAMAMADGLICEKPVVSPNQNARPCKTPCSLRTSCANCTSQAMECMWCGSTKRCVDSNAYVISFPYGQCLEWQTGDCVSQNCSGLRTCSQCLEQPDCGWCSDPSNTGKGQCIEGSYRGPMKAVGKQSHDMILDQGLCPKEKGFEWAFINCPACQCNGHSTCVNNSVCEQCRNLTTGQQCETCMPGYHGDPTNGGKCQACKCNGHANICQVLTGKCYCTTKGIKGDQCQLCDSENRYLGNPLRGTCYYNLLIDYQFTFSLLQEDDHHYTAINFMATPEQTNKNLDMSINASNNFNLNITWSVGSTAGTISGEEVPIVSKSNIKDYRDSFSCEKFSFRSNPNITFYVYVSNFSWPIKIQIAFSQHNSIMDLVQFFVTFFSCFLSLLLVAAVVWKIKQTCWASRRREQLMRERQQMASRPFASVDVALEVGGEQSDLLRGPLEGAPKPIAVEPCSGNKAAVLTVFMCLPRGSSGVPPPGQSGIAIASALIDTSQQKAMECKEKSLGLKNRKQPPPAHQGTCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6L0</accession>
    <name>W5N6L0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 2, facilitated glucose transporter member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Fructose transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glucose transporter type 5, small intestine</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="1">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015340345.2"/>
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        <property type="gene ID" value="ENSLOCG00000013204.1"/>
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    <dbReference type="KEGG" id="loc:102691862"/>
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    <dbReference type="eggNOG" id="KOG0569">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000167279"/>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GLUT"/>
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    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
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    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF99">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 3"/>
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    <dbReference type="Pfam" id="PF00083">
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        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
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    </dbReference>
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    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 14" id="KW-0813">Transport</keyword>
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            <end position="22"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="328"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="484" mass="53168" checksum="68883B019C037E98" modified="2014-04-16" version="1">MESESSPLTGLLLTSIFSAVLGSLQIGYHTGNINAPERVIIKVFNSSWQSHYNTSISETRLAFLWSLSVSGKDFGAVLGAVGVKWLADSFGRRNSILIANALSIAASLLMSIAQFCMWFEALILGRLIFGMFCGLAMSLNPLYIQEISPTALRGAFSTLNQISFTTGILLGMVMSLNVVMGSESLWGLMLQLSLVPAAMQYFTLAFCPESPRYLLINLGEEVEAETALRRLRGNSGDIRAEMMEMKQESSFSPKCITVREFVQKQSYREPIMILTVICLGANFSGFNAMINYSTDVFLRVGFEEAEYLTLGVGSVNVLFSIVSFFLVETAGRRKLLLVGFLALGVCNLLMSISSALLNIFPQMAHLHALLVFGLIAAYESGPGPISWFIGAELFNQSARPVAMSFSSILNWGSKCLLAFIFPPLMGIAGPYTYLVFMVFALIAFFFTLARVPETRGRVFNDIAAEFQQTDSLLLTPKKLKSSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
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    <name>W5N6P7_LEPOC</name>
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        <submittedName>
            <fullName evidence="7">Thyroid hormone receptor interactor 11</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="entry name" value="GRIP_dom"/>
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        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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            <begin position="1888"/>
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            <end position="973"/>
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            <end position="1174"/>
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            <begin position="1520"/>
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            <begin position="586"/>
            <end position="602"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004555"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50913"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016306.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1973" mass="224441" checksum="B71C3F7F3385B2B1" modified="2014-04-16" version="1">MSSWLGGLGSGLGQSLGQVGGSLSSFTGQLSSFTKDILLEGTEEVGDAATELHVSNSKLMEVESTYTTLKSEYERLRKIHLELEEKHEAAEIQIKQQSSQYRSLLQQKEVEISHLKARQNSLQDEVQKLQMAAQPTEPAVLPTSTTASFLPTPGPHPGFQGDEMDFGDVIWSQQEINRLSNEVSRLESEVSHWKHLSQTSKTQGAVSTDQSDVQALQRTIKELKETMSRDIDEHQHELSVLQDAHRQKTAEITRRHREELAEYEERIEELEEQLQAGGSSTGTQEHSQVLELQRTIQALEESAQEQEKKLKSMAESLAASGKKQTALLQEKELAEAENNQLMQNYRRLQNSVEELQTRVTEQEEKAMHKSHLENEITGLKKALSAAEKEISRLNSLSATESQAEVEHADILELNTIIESMRKEKEELEQQKLELLERLKSQEMQENGTRKNEELDAVLLEVEELRSELQEKELAFTEGAMEKDRLAAEVEELDRQNQEANMHLISLKDQLAQQRKDADASICRLQSELESSRKQQKDQAAMQQELEQQREKLSQSAFALNDMHMVRQQLESTVGDLKDKLKKLQEDSSHLRKENSELKKQLQETEEQLSTARSELAGSSKSAVRDEQQEQLLQQKEKEMSELKQEMSENKGLLEKVSSANYELKMENKKLKVAHTEATVKHEGLSRQVQDLQAALKKASLERDTKVEALRLEKRQLEAELNQAENRLAEQARQYQQTIMELTKARSMDATALQTEHERMVKLNQEKDCEISDLKREIEQMAADHQQTSEILSTSLCGQKQLTEVIKEKEAFIEKLKERVGETQEELEKCDRAAKEGDMIKHLLDEKEKQLGVMKEENSHLKEEIDRLRDQQSRPQPIAEPKTLDIITELETEITQLKSKNNHLEEEILALKKVMEEQKGSLLQSQQSIQMQQNELNQAKMRHEQLTLNYEKLISEKDQEIVHLQESIDQLKQQRETQVINTDSSNILQEDKTQSMNVENGNEKHDFSKVEIERLVKGIKEKEIEINLLNEKNVSLTKQLDQLVVSKDEVGKLSQIVQQKDLEIQALHARVSAGSHMQDIMYLQQQLQAYAVEREQVLAVLNEKTRENSQLKSDYHRIMDIVAAKEAALLKLQEENQRLSNMSDTGSQEMVRETIQNLSRIIREKDIEIDALTQKCQTLLTVLQTSNSGSEAQSSGVSSNQFEELLQERDTLKQQVKKMEEWKQQVITTVKNMQHESAQLQEELIKLQSQISADSDCNSKLSVDYNRLIQGYEQKEKKLGNLSQELAQVQQTICQLSNTKDVLLGKLDLVASSPDSGTPSVSEAACKVTTPLEQEDTVKRLQREQEHLQKILQEKETVIRTLQENNHRLSNSIASASESEQRGHEEAAAEIRQAKDKLGALQTSLREKDLLIKSKGDQLSSVSESLRNKENDNEVLKQAVTNLKERTLILEMDIRKLKDENEQIIAKSREKETEFRALQETNMQFSMMLREKEFESNSMKEKASALEKMLKEREQGKSGELNQLLNEVKSMQEKAVKFQQERDQVMLALKQKQMETSALQNELQHMKDKEQRLKQELERLRTHLIEIEDSYTREAVAAEDREAELRRKVVLLEEKLASSSSAVESASHQASLQVESLQEQLNTVARQRDEALMQLRTSQEQVNQYAVSLSNLQMVLEQFQQEEKAMYSAELEKYKKERAEWKRKAEKLEDRLAAMQINLDEANAALESASRLTDQLDLKEEQIEELQKQVALRQEMLEEAQNKLMNLLNSTEGKVDKVLMRNLFVGHFHTPKNKRHEALRLMGSVLGLKKDEMDKLLLEEPHGVTGWVSSWLGGRTAQSVPNTPQRPGHSSQMNSSFSEMFVKFLEVESRPTAPPLRLPVYDVKPLTAHPPGQTYSSAGSSASGASGGAKRPESSPFLAPRSAAVPLMNSAAAGAGGGGHLLMKPISDSLPTFTPVPVSADASAGAVLKDLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7M1</accession>
    <name>W5N7M1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 Z</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein Z</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin-protein ligase Z</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01031010">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7M1"/>
    <dbReference type="FunCoup" id="W5N7M1">
        <property type="interactions" value="1038"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016630"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016660.1">
        <property type="protein sequence ID" value="ENSLOCP00000016630.1"/>
        <property type="gene ID" value="ENSLOCG00000013488.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159091"/>
    <dbReference type="HOGENOM" id="CLU_1079844_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7M1"/>
    <dbReference type="OMA" id="MIAHTIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013488">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23809">
        <property type="entry name" value="UBCc_UBE2Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000263">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF26">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="147"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="258" mass="26352" checksum="4B0E60C1F7E92963" modified="2014-04-16" version="1">MAESAGEEASGIGVTGSTHSGGGAQFSPGSGNSVPGDPSGAAPDSVPSAAAAPETVPPVSTPDIPSLPASAIGVLGTAVGTAILAPSPGLGPAAPAMVGTAVLSPSIGLGVTGTPNLAGAGLLSQIHSTFWDPTVSSDWDSEKPSQQCILRIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKLITTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5N970</accession>
    <name>W5N970_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N970"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017210.1">
        <property type="protein sequence ID" value="ENSLOCP00000017179.1"/>
        <property type="gene ID" value="ENSLOCG00000013937.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYH5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000186894"/>
    <dbReference type="HOGENOM" id="CLU_031817_0_0_1"/>
    <dbReference type="InParanoid" id="W5N970"/>
    <dbReference type="OMA" id="RGEAMEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052560">
        <property type="entry name" value="RdDP_mobile_element"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688:SF1">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="74"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="400" mass="45082" checksum="21DC52D1E9DA7959" modified="2014-04-16" version="1">KSNIKIYGYHLIGAIHHVHHGVATLVKLDKNAEHVAQSWDEDTTQWFAIEIEGLTTVNIYKQPSTQFSSLPSYAGDFNCQYIKWGYQKSTTEGQALADWASNQGLILLSDKKQANTFFSARWQSGTNLDLVFFSPTNNHMPSPQHSVLSRFPNSQHRPVVTLHSALVTPTSSRSSTRWNFRKANWTKFTKETSSFPLKLPDPNSTDVKDSRFCNMIISTSNNHIPRGFRKTYISGWDEECVNLASELESATTTERAQSISLALIEYLDNKRKQEWNNTVSEIDMKHSSRRAWQTIYQLTGQGHKPQIQNQVKANDVAKVLVDSSKPTHIDSLPEEIHNSWPASSADDNSHCTFTYPELIAAISTLKAGKAPGPDHLHPEQLTHLSAPALEWLLPFLNNCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N980</accession>
    <name>W5N980_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01002194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015201210.1">
        <property type="nucleotide sequence ID" value="XM_015345724.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N980"/>
    <dbReference type="FunCoup" id="W5N980">
        <property type="interactions" value="1151"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017189"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017220.1">
        <property type="protein sequence ID" value="ENSLOCP00000017189.1"/>
        <property type="gene ID" value="ENSLOCG00000013942.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684519"/>
    <dbReference type="KEGG" id="loc:102684519"/>
    <dbReference type="CTD" id="51201"/>
    <dbReference type="eggNOG" id="KOG1315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153716"/>
    <dbReference type="InParanoid" id="W5N980"/>
    <dbReference type="OMA" id="CFVVMHI"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013942">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016188">
        <property type="term" value="P:synaptic vesicle maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="131"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="347"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="388" mass="44518" checksum="5909205435A50B90" modified="2014-04-16" version="1">MVSKLKMAPSGSGGIKRRCQRVLYWIPVLFITIIVAWSYYAYVVQLCIESMENTGEKIVCLVAYHLFFLMFSWAYWKTIFTKPLNPLKEFHLSYADRELLEREDRGEAQQEILRRIAKDLPIYTRTMSGAIRYCDRCQLVKPDRCHHCSVCDKCILKMDHHCPWVNNCVGFSNYKFFMLFLAYSLLYCVFIAATDLQYFIKFWTAGGNIHKHLIQYVNGLPDTQAKFHIMFLFFAATMFSVSLSSLFGYHCWLVSKNRSTLEAFRAPAFRHGADKNGFSLGLSKNFRQVFGDEKQYWFLPVFSSLGDGCSFPTCLVNQDPEQPATPAGHNPLVKKCDAGETHQFPAKTLRESQSRLLNAGQSWTESEGGDDKDKLGMSNPAMTIENET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9C8</accession>
    <name>W5N9C8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9C8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017268.1">
        <property type="protein sequence ID" value="ENSLOCP00000017237.1"/>
        <property type="gene ID" value="ENSLOCG00000013984.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165987"/>
    <dbReference type="HOGENOM" id="CLU_015935_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9C8"/>
    <dbReference type="OMA" id="FSMQEAQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013984">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000122">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000015">
        <property type="entry name" value="dnaJ homolog subfamily C member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF1">
        <property type="entry name" value="DNAJ (HSP40) HOMOLOG, SUBFAMILY C, MEMBER 3B PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="7" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867432" description="DnaJ homolog subfamily C member 3" evidence="12">
        <location>
            <begin position="35"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="341"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="13">
        <location>
            <begin position="395"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="452"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="347"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="501" mass="57050" checksum="58385AF3B1C71489" modified="2014-04-16" version="1">AAMEASRRKGLSGILSSLSLLCVVLDLQIDGVLGASHVEIEHHLEMGRKLLAAGQLAEALSHYHSAVEGDSKNYLTYYKRAAVFLAMGKSKSALPDLSKAIELKPDFLAARLQRGNIFLKQGSTQEAREDFEAVLRGNPGHEEARAQLAHADELDALVEEAHHAHQRGDYHGTVRVLERVIELSPWDPESRELRADCYMKLGETRKAIQDLAPTTRLRNDNRAAFLQLSRLHYSLGEHEDSLRGVRPASLWADPASPVSLPSSQQVKKLSKQLDSAEQLISESRYPEAIEKYESVMKTEPNVPFYTTRAKERICHCLSRNRQAPEAIDICSEAHQRDPRNILILRDRAEAFILNEEYEKAVEDYQEAREMDGDNQEIREGLERAQKLLKQSRKRDYYKILGVHRNANKQEIIKAYRKLAQQWHPDNFQSEEEKKQAEKKFIDIASAKEVLTDPEMRQKFDAGEDPLDPENQQGGGGHGHGDWPFEFNPFRSGNFHFKFHYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9Q0</accession>
    <name>W5N9Q0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Selenoprotein S</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the selenoprotein S family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198682.1">
        <property type="nucleotide sequence ID" value="XM_015343196.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198685.1">
        <property type="nucleotide sequence ID" value="XM_015343199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9Q0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017359"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017390.1">
        <property type="protein sequence ID" value="ENSLOCP00000017359.1"/>
        <property type="gene ID" value="ENSLOCG00000014088.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107076905"/>
    <dbReference type="GeneTree" id="ENSGT00390000015688"/>
    <dbReference type="HOGENOM" id="CLU_1421018_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9Q0"/>
    <dbReference type="OrthoDB" id="75792at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014088">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036513">
        <property type="term" value="C:Derlin-1 retrotranslocation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036502">
        <property type="term" value="C:Derlin-1-VIMP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009703">
        <property type="entry name" value="Selenoprotein_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28621">
        <property type="entry name" value="SELENOPROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28621:SF1">
        <property type="entry name" value="SELENOPROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06936">
        <property type="entry name" value="Selenoprotein_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="65"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="191" mass="21555" checksum="97536CE0869AE2BC" modified="2014-04-16" version="1">MEYPLAKTPGPGSGISQDDRLKAARLRMQKQQDALVRQTELEKPSEVCAPSPSVKADSSHNQVLKAERDPREHVAQDDARRAAWLRMQDKMDADAEHHKARLMEEAEKKQQRDKEAEEQKKKEKIEKWEFMQEGKSYRGKAKAAQQETSELAAVPKKTRDKKPLRSNDYNPLSGDGGCGSSWRASRRSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9Z6</accession>
    <name>W5N9Z6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pleckstrin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01022325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632752.1">
        <property type="nucleotide sequence ID" value="XM_006632689.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9Z6"/>
    <dbReference type="FunCoup" id="W5N9Z6">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017455"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017486.1">
        <property type="protein sequence ID" value="ENSLOCP00000017455.1"/>
        <property type="gene ID" value="ENSLOCG00000014169.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689472"/>
    <dbReference type="KEGG" id="loc:102689472"/>
    <dbReference type="CTD" id="26499"/>
    <dbReference type="eggNOG" id="ENOG502QYJ8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157229"/>
    <dbReference type="HOGENOM" id="CLU_067828_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9Z6"/>
    <dbReference type="OMA" id="VVRNWKV"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014169">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000208">
        <property type="entry name" value="Pleckstrin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000243">
        <property type="entry name" value="Pleckstrin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF2">
        <property type="entry name" value="PLECKSTRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="9"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="5">
        <location>
            <begin position="143"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="251"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="357" mass="40440" checksum="4C65DA25B5C77EE4" modified="2014-04-16" version="1">MDTMQPENAILKEGFLVKRGHIVQNWKVRWFVLLPDRLLYYKYDCSRKDSSLRGKVLLLGCQLTCPCLEYENRPLVIKLRTQKSAEYFLEACSREERDTWADSITAVIQNLCPGKAQKTLSEQTPLQMNNISLNQVVDSMHDVHSGIKLTNNVEQGSYFKNCFSGSAMVDWLVSMSFVVTRKEAVTLASALMEESYVKPVGIKSMEAIRNSNMTEQFLDDSTSLYALAESFKRKASVKSGASLSALELSGKVVKRGYLLKQGHKRKNWNVRLFVLRTEPGYLHYYDPSREHVSPVGGFPLRGCLISSLDDNGVPSGVKGKVQGNLFKIITQNDTHYFIQATSREEKMDWIVAIKQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBG8</accession>
    <name>W5NBG8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">threonine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01004956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBG8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017977"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018009.1">
        <property type="protein sequence ID" value="ENSLOCP00000017977.1"/>
        <property type="gene ID" value="ENSLOCG00000014607.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154969"/>
    <dbReference type="HOGENOM" id="CLU_008554_0_3_1"/>
    <dbReference type="InParanoid" id="W5NBG8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014607">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000002">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF51">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="405"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="779" mass="88475" checksum="A9FB2659673F2124" modified="2014-04-16" version="1">MAETIVARLAAQEEQVRLLTQEVSWLRGGVGGDPAAVAGLCSSPELETLRSENEKLRYRILHLRRSLQSESDPETGAREGEEGKETRAGKSSSREVPGNLTPNLLCLKGSASCSHGSGYRRSEDSSLLRQARHDHISVWRHRDTVSSGGAEVSSPCEEAAPGLQSLAGDVAWLAVLQFSCLTTHGWPDTAGCGGVLGTYYWGFSAPESRIMKCRLLILRFDHTKTDCIERSNTLILHLNWCRSSFVQSSCLTLGVCLSAGQLRLPQCSGDEEREAGELQGRAAIRRVHMLISHVFNTRNELFHFLKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIYKNSSTYWEGRADMETLQRLYGISFPDSKMMKEWERFQEEAKSRDHRKIGKEENPVHFRRGNAVIETQLCSWSRGCHNGRIHLRKTWSLSGHWQHYSDNMFSFPVEQDIFALKPMNCPGHCLMFSHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIENEMKGCLEFLRCVYAVFGFSFQLHLSTRPEKFLGDIQIWDQAEKQLENSLNEFGEPWKLNPGDGAFYGPKIDIKIKDAIGRYHQCATIQLDFQLPIRFNLTFVDKEGNDKVRPVIIHRAILGSVERMIAILTENYAGKWPLWLSPRQVMVVPVNPACEPYAQQVCQQFVEAGFMADVDLDSSCLLNKKIRNAQLAQYNFILVVGEKEKATGAVNVRTRDNKVHGELALAEALARLRRLRDSRSRRAEEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCA3</accession>
    <name>W5NCA3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat domain 62</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023881">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCA3"/>
    <dbReference type="FunCoup" id="W5NCA3">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018294.1">
        <property type="protein sequence ID" value="ENSLOCP00000018262.1"/>
        <property type="gene ID" value="ENSLOCG00000014830.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165793"/>
    <dbReference type="HOGENOM" id="CLU_002067_1_0_1"/>
    <dbReference type="InParanoid" id="W5NCA3"/>
    <dbReference type="OMA" id="QEDGCGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014830">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000046">
        <property type="entry name" value="WD repeat-containing protein 62 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055292">
        <property type="entry name" value="MABP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056161">
        <property type="entry name" value="WD40_MABP1-WDR62_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056162">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44813">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44813:SF1">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24780">
        <property type="entry name" value="WD40_MABP1-WDR62_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24782">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MABP1/WDR62 first WD40" evidence="5">
        <location>
            <begin position="58"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MABP1/WDR62 second WD40" evidence="6">
        <location>
            <begin position="391"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="696"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="772"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="830"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="871"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1096"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="781"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1124" mass="122547" checksum="7360C7D7A65E9931" modified="2014-04-16" version="1">MAEGAARGGGLGSRVRKLLGSPPALSLRRRSRQPPARRSVLGRVTLEKVLGITTSGASGLACDPSSGLVAYPAGCVVVLLHPKRNKQSHILNTSRKTFTALAFSRDGKYLVTGECGHVPCVRVWDVAERTQVAEVQHHKYGVACVAFSTNGSYIVSVGYEHDMTVNVWEWRKGSVIASNKVSSRVTSVSFSEDSSYFVTAGNRHVKFWYLDASKERRVNGTVPLIGRSGLLGEQRNRLFCDVACGRGQMAASTFCLTSSGLLCQFNEKRLLDSWIDLKTSAGSCLAVSEQLVFCGCTDGTVRVFNPCTLDYITTLPRPHCLGVDVAKGIAPGHLFSWHPEAVYPDTVALTYDPVTCWLTCVYSDHSVYVWDVRDVRKVGKVSSALYHSACVWSVETYPELEDSSSGCLPPGSFLTCSSDNTIRLWHCDTGPPRPHLTGYRRNLYSHDLLKIVYAGNSVQHLKDGPERGEVNGADSKAGIRVLGISPDGQHLASGDRGGNLSIYSLQFLDEVVKVEAHDSEVLCLEYYSPEPGTTLLASASRDRLIHVLSAESDYSLEQTLDDHSASITAVKFTGALPELRVVSCGADKSIYFRTAEKTSEGLSFSRSHHVVEKATLYDMALDATRTHAAIGCQDRNVRVYDVRSGKLSRCFKGSLSDEGTVLKVQMDPSGMFLATSCSDKNICIFDFYSGECVATVFGHSEIVTGMRFSQDCRHLITVAGDSCVFVWRLDSQMTNSMRKRLAEVRRAGRREGAPKTSAEANFIRRQTYITVPSSQPAQTGEEGEMEEDEQEEECLQTPARDTSDPGFVDPAFLQTNGRLPLWAKRLGAAESCAEEPPERLPGSPYQPRGRWAEQSDPQALRSVLATRLQLPLSPSPPREQGPASGGPDPEDTDFQPQSLDSLIGEEEEELSESEETFPADPDLPETSDDILYPANSTALSTVADGDFDVKELCRGGGVRRRPVEHSPDSACCVGSAESQASSQDRPGDGITPPPPPPPVSTRTTVPPLSLPLLPPDADSLSQEEEEEETSPSLAMTPEQETLLHRHFGTLADDLANEKFDTDLRDLRPSAESIFLNPRLSISARFLSRCQARSRLGAALPPRPFAPHAGISEEEPSDGTLSQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCU4</accession>
    <name>W5NCU4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCU4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018485.1">
        <property type="protein sequence ID" value="ENSLOCP00000018453.1"/>
        <property type="gene ID" value="ENSLOCG00000014989.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183115"/>
    <dbReference type="HOGENOM" id="CLU_074596_0_0_1"/>
    <dbReference type="InParanoid" id="W5NCU4"/>
    <dbReference type="OMA" id="QMCYVGE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014989">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF7">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="10">
        <location>
            <begin position="141"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="269" mass="30972" checksum="AB05D01C763CF010" modified="2014-04-16" version="1">MVKIAFNSSLAQKALGKEGDVLVAEKARFVLPVRPRIYLYEKHDDPEVATEYRNENSTGRCLLTLLGLAFILTGLIVGGACVYRYFAPKKLYHGGMHFAEMDNNLVPMDLETREPYYLPRIDEEVEIRDNVAIINVPVPQFAPTDPAAILHDFDRKLTAYLDLNLDTCFVIPLNSSVVMPPEDLLDLFVQLMTGSYKTYLIHEDLVVTERIDDVTELGFYIYNLCDDKDTYRMQHRSEIIGLQKRSLEDCFTIRHFENKFVTETRICKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE09</accession>
    <name>W5NE09_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE09"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018868"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018900.1">
        <property type="protein sequence ID" value="ENSLOCP00000018868.1"/>
        <property type="gene ID" value="ENSLOCG00000015335.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158553"/>
    <dbReference type="HOGENOM" id="CLU_1266527_0_0_1"/>
    <dbReference type="InParanoid" id="W5NE09"/>
    <dbReference type="OMA" id="DDIQETH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015335">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF15">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="218" mass="25019" checksum="17DE19A71DB7B379" modified="2014-04-16" version="1">MEEDEMLARHLQAQFDREEQLDQDSLSRRSTQRSHPSAQNGRLDYNLHFHPMLTANHFVATFPQLSGLGENRASRQRHGRPRQTQNAVQNLFDDSQGNNYEAILAFEDHQGAVVSKPKLSKREIERLPTKPFNPAYSAGKTECQICFSDYREGEQLRVLPCLHDYHAKCIDRWLKVFWSIVLMIGKNAGMLEKPHSTALSKGACKTAIRSLHSSIRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE90</accession>
    <name>W5NE90_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 9">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ADSS2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 9"/>
    <dbReference type="EMBL" id="AHAT01027837">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE90"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018949"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018981.1">
        <property type="protein sequence ID" value="ENSLOCP00000018949.1"/>
        <property type="gene ID" value="ENSLOCG00000015399.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015553"/>
    <dbReference type="HOGENOM" id="CLU_029848_3_2_1"/>
    <dbReference type="InParanoid" id="W5NE90"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015399">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000011">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF13">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE ISOZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="38"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="333"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="365"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="447"/>
            <end position="449"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="50337" checksum="A0CED2804A5F02F2" modified="2014-04-16" version="1">MSDGGSADSAGARNGAGTGVVNRGVGNQVTVVLGAQWGDEGKGKVVDLLAQDADIVCRCQGKKNLPLKVISAKVVIQLGLLQNINPQIKSIGPQGNWLTIRKQYLNEFGEFPEIAGTELHWGWGCACISEQRAATFDHLQGLSGLHFPQKRETEIEIGNATGEAGEGDVGRGWRESVVLMGLEMANILSFLEGFQERFKVLAQQYKSMYPTLEIDIDGELAKLKIYMEKIKPMVQDGVYFMYQALHGPSKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVCTGLGMPPQNVGHVYGVVKAYTTRVGIGAFPTEQNNEVGELLQKRGKEVGVTTGRKRRCGWLDLVLLKYAHMINGFTALALTKLDILDVFPEIKVGVAYKVDNELIPHFPANQEVLQKVEVQYETFPGWNTDISGARTFEELPENAQKYVRFVEKHLSVPVKWIGVGQSRESMVQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFA6</accession>
    <name>W5NFA6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial genome maintenance exonuclease 1</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MGME1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MGME1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01037501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFA6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019315"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019347.1">
        <property type="protein sequence ID" value="ENSLOCP00000019315.1"/>
        <property type="gene ID" value="ENSLOCG00000015691.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVKE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003349"/>
    <dbReference type="HOGENOM" id="CLU_068902_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFA6"/>
    <dbReference type="OMA" id="DCVAKYQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015691">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008297">
        <property type="term" value="F:single-stranded DNA exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.320.10:FF:000005">
        <property type="entry name" value="Mitochondrial genome maintenance exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03030">
        <property type="entry name" value="MGME1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31340">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE EXONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31340:SF3">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE EXONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="344" mass="38326" checksum="8422699ECDA70116" modified="2014-04-16" version="1">MRIYRLLSAVWSAKSRSLSVALVSQGSFSVVRCLSTSCPWNGRRKGSQYDSVDTEKYSSLVHSVVSSKVSYRTPASLEEEDGQLYGPVVKSKPAAKGTEAKTLKNIVPLLNNAKVLEQQTVEAELPPPMRIALRKAQGRPAAPSVTRILQHSMPLEQAFYLERWRKRMIGELGEEGFKEYTANLFRQGNQFHAALEAILMSTEVPEEAEAPAGGVCGYVQSVQHVLADISGVRAIESAVTHQTLQYVGLVDCVAQYRGKLCVIDWKTSEKPKPLLHNTFDNPLQVAAYIGAINNDDNYGFQVEDGLIVVAYKDGSPAHPHFMDSALCLEYWTKWLFRLEEYLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG71</accession>
    <name>W5NG71_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Carboxypeptidase A4</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG71"/>
    <dbReference type="FunCoup" id="W5NG71">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019630"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019662.1">
        <property type="protein sequence ID" value="ENSLOCP00000019630.1"/>
        <property type="gene ID" value="ENSLOCG00000015944.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160121"/>
    <dbReference type="HOGENOM" id="CLU_019326_0_0_1"/>
    <dbReference type="InParanoid" id="W5NG71"/>
    <dbReference type="OMA" id="WSYDSGI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015944">
        <property type="expression patterns" value="Expressed in liver and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03870">
        <property type="entry name" value="M14_CPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000132">
        <property type="entry name" value="Carboxypeptidase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000001">
        <property type="entry name" value="Carboxypeptidase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034248">
        <property type="entry name" value="CPA_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF71">
        <property type="entry name" value="CARBOXYPEPTIDASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869672" evidence="14">
        <location>
            <begin position="17"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="15">
        <location>
            <begin position="121"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="428" mass="48580" checksum="7C610C7D71BCB7C2" modified="2014-04-16" version="1">MKELLFFFALLLAVHCQKRFIGDQVLRIEPGSNEQISVLQSLQEEDDFQLDFWLHPTNPDLPVDVRVPIASLQAVKDFLESNGIRYSVMISDVQEMLEQEQKEMEANRRMERSSKSFNFAAYHTLETIYDWMDTTIKVLRYCTMIVTHYRNSLLDYENFETPCCLLVFQFSTGSSSRPAIWIDAGIHSREWVTQATAVWTANKIATDYGQDRSLTSILDMLDIYLLVVANPDGYAYTHSNNRMWRKTRSKNPGSVCVGVDPNRNWDAGFGEGPGASTNPCSDSYHGPSAQSEVEVKNVVNFIKQTGNFKGFISIHSYSQLLMYPHGYSCNNIPDYQELDAVGKSAAKALGSLYGTSYKVGSICKIIYQASGGSIDWSYNYGIKYSFAFELRDTGRYGFLLPANQIIPTASETWLALLNIMEYVRDHTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGB2</accession>
    <name>W5NGB2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Isthmin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isthmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGB2"/>
    <dbReference type="FunCoup" id="W5NGB2">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019671"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019703.1">
        <property type="protein sequence ID" value="ENSLOCP00000019671.1"/>
        <property type="gene ID" value="ENSLOCG00000015977.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR6G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159062"/>
    <dbReference type="HOGENOM" id="CLU_030263_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGB2"/>
    <dbReference type="OMA" id="FRQQNGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015977">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000033">
        <property type="entry name" value="Isthmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051867">
        <property type="entry name" value="Angio_Inhib/Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239:SF30">
        <property type="entry name" value="ISTHMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867723" evidence="7">
        <location>
            <begin position="27"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="8">
        <location>
            <begin position="287"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="462" mass="51966" checksum="9B5C1EC5D3917B19" modified="2014-04-16" version="1">MVRLAAELLLLLGLLLLTLHITVLRSSPLQQGNATSLNREQDIVLTENNLSDGGSSSTHHSSGERHGHPEHRAAHRPLPKHRSSQDNTGSSIQRDGPGSFLLDLHNFPDLSKADINGQNPNIQVTIEVVDAAEEEPDKELQKENKSSWPSPSWRNWWQLSTSTARHGDSQQEYTYDANTDDSNFLKPLSDWDRRGGPSQRSFEAKAQAEYDYIDGEGDWSSWSACSVTCGNGNQKRTRSCGYACTATESRTCDMPNCPAPGIEDTFRTAATEVSLLAGTDDFNATELFGVDTDSCERWMNCKSEFLKKYMLKVANDLPSCPCAYPTEVAYSTADLYDRGMRKEFRWKDASGPKEKLEIYKPTARYCIRSMLTFESTTLAAQHCCYDDTMKLITRGKGAGTPNLISTEFSADLHYKVDILPWIICKGDWSRYNEARPPNNGQRCEENPSDEDYYKQFQEAREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGL5</accession>
    <name>W5NGL5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATP-binding cassette, sub-family H, member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019774"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019807.1">
        <property type="protein sequence ID" value="ENSLOCP00000019774.1"/>
        <property type="gene ID" value="ENSLOCG00000016051.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166587"/>
    <dbReference type="HOGENOM" id="CLU_014367_1_0_1"/>
    <dbReference type="InParanoid" id="W5NGL5"/>
    <dbReference type="OMA" id="SRYFIER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016051">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038:SF3">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 20 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY H, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="602"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="19"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="8">
        <location>
            <begin position="454"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="684" mass="76489" checksum="CFA3CF59E545F1F1" modified="2014-04-16" version="1">EKMTDNTEASLPTWTESAIKCRGVCRSYGKLKVINNLHLTVPQGHILKDLLKLKCLNTPRISRTGIPNSVPQEGXLLQDLALYNEFTISDTFWFYGRIHGLASKETEARMTFLVDFLDLPDKSRLIRNLSGGQKRRVSLGAALLQKPELLILDEPTVGVDPVLRAKIWQHLVEIVKDGQVSVIITTHYIEEARQASVVGLMRHGRLLAEAPPDVVMRQHCAVTLESAFLQLCESSDQTSSNLNIGPEGKDKEASQSSEDSKDECRPILGNMQEGDPRFTVDWKVRARQVLPKCRNIWALMIKTFVRMKRMPGFLCFQFLLPVIQISLMCLCIGGDPRGIQVAVVNNETGNSSFSHSLLSFIDNTTVHQVFCRIPISFLLQVIVQQSEAFTGVRDGRYWGIIGFGSNFSTDLMMRMLQKSVTKQVVEGGTVHVWLDMTNRQIAFMLQKKLQEAFESFVQDKLGTLSYLVSLPVKFEEPIYGSSNPDFTSFVTPGAVLSITFYLAVGLTALSFVTERKEGLLDRCWVAGVSSLETMLAHLLSQLVIMSVQVTLLLLFVLLVFKIPNEGSLALIVILIFLQGVTGISFGLVISAAIDDEQSANQAALGIFYPNLIISGIIWPMECIPYPLRYLSMVLPQTYAAEALRCIMYKGWDLTAMMVWRGFAVTLGWNTFFLILATVILKMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGL7</accession>
    <name>W5NGL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Usherin</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008472">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008474">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008476">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000019809.1">
        <property type="protein sequence ID" value="ENSLOCP00000019776.1"/>
        <property type="gene ID" value="ENSLOCG00000016044.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158456"/>
    <dbReference type="HOGENOM" id="CLU_000067_0_0_1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016044">
        <property type="expression patterns" value="Expressed in muscle tissue and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:004110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002683">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003132">
        <property type="entry name" value="Usher syndrome 2A (autosomal recessive, mild)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000224">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000275">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000313">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000412">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000111">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000126">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000069">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000819">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000915">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000991">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001004">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001023">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001030">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001037">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001052">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001085">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001099">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001100">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001161">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001168">
        <property type="entry name" value="Usherin"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
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    <name>W5NII9_LEPOC</name>
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            <fullName evidence="11">Forkhead box protein M1</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="10">Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Also plays a role in DNA break repair, participating in the DNA damage checkpoint response. Promotes transcription of PHB2.</text>
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        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIK9</accession>
    <name>W5NIK9_LEPOC</name>
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        <recommendedName>
            <fullName evidence="3">Centrosomal protein of 162 kDa</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
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        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the CEP162 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
    <sequence length="1355" mass="154335" checksum="10473D1ACDBDA7C8" modified="2014-04-16" version="1">MSRRFTKEELDEQFEQFLKESVSDDSIDLGSSQRPSVLDSLGKAAKKETANAPPRPWWNSDDDDNDVSSGRGMLGTGKNFLKSVRSSQPIKEEDEDQKEKKPGDHQETNPVSFSRDSLEPEESVVASGPDQSAAGFGMDTLDEEEEKARFFAKLESGVSSTIDYSKLNKELDSTGSTVIATLRYKSWTIPLCSKISFSASVSPNYSEDFEDDTSEKEGHDQKSEAPAMLAKVSLLDSLESTAEAQKRNITNEKADKEMENTEPREQEMTGTGRDAVSYGQSGASDMEALHEAYRKINYSFGDSDGYHEIPEHLHSLQMLVTRQKPVSDLPTAEELMKPIGVGPGFARGFALQPVSTLVPLSSNFGHILCINWSPPSAASDDYFSGGPNKENFSGILRAREDTKTVKQSKIADEVDHLMNPQTPSKQIKSPQTKNKKTVSGSSQSTKSYGFSKSSSSVKQSSPASERKTLNQGMKRNSTKHKSPADPAAVRTKVQSYTNIYTGGDSQALHPTQSGLKMNNELIASVQSFAAFLQQQVMLSPLQDSGSREQDRVEHRNMSVKKSEEAQERWSSEHVLVEELKAQLIQKEKELKNQEEELKFAHSKEVFNLKQENYILQTKVNELAYSGKKRSWKIYSATDPVTEDKLKLIEKELKEQEILIQGYHQENEKLYRQVKELQNQNKLNEDKMFKENQRLMTELASTKEQITRNNLQKVPDNGSEQSRDHSFTELLTQLRAAQGGEARLMEEVRRLKQDKQSLELDLEVMKKERDLAKAQAVYVSGDKTFEMKVMGEKYNQEIADLKKRLQWYAENQELLDKDTMRLQAATSEIEKLKEQVEKLKAEVGNRNIQQQRKMKERVGDAKRIQDLERQVKEMEEIIRRRHPNSLPALIYAAASAGEGGAAAKPETVTFLEKRVQRLEAELQGKDEEAKRSLRAMEQQYHKIKIQYEQRISELEQLLSHKLIPEREESDQWAAEAGALGEELKQLREAYQAKEDALQREIETLQNQLLPTKQKVAENCRKSPQRPEKQVEGTYKIKLDKLNQEVAAKNKTIQELSRTVEKLQKERRSLLSRQTGDGKMNKNKSVPRADRNSTGAVEGFPATWDEKDYQPHAFAGSHISEVLQDNERLEREVERLALDMDQQRVKLQAAVAQAECEARRTQEETAEYVSSLKAEHQKELEHLVTHHALEHSSSKVAELSNKVSTQEIMIRHLRDQVSELQKTKEALAVLHVREETLQTQMAKLLEELREAKEAHTPELKHFSALERKIKNMEIRHLQREQELQQIIEQTRRIVHEEQQQEVSKWKRLAQQKTGELEAFRVELDSILDVLRELQRQGVVIPAPHTAAQRVTGLDWRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIR6</accession>
    <name>W5NIR6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATPase family AAA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NIR6">
        <property type="interactions" value="1564"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020525"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020560.1">
        <property type="protein sequence ID" value="ENSLOCP00000020525.1"/>
        <property type="gene ID" value="ENSLOCG00000016615.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074694"/>
    <dbReference type="InParanoid" id="W5NIR6"/>
    <dbReference type="OMA" id="DKQCTTN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016615">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000021">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000699">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF5">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="972"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="229"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="714"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="929"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1079"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="731"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1127"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1157"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1181"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1273"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1284"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1312"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1323"/>
            <end position="1336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1432" mass="161894" checksum="B75F58AC9D4FCD81" modified="2014-04-16" version="1">MVNTRKSSQPELRSHYISSRTRSSVKNNPSTEDHHHRVHKCWHQDRCQLRAPKDQGSDVKSSSARLSPPAKRTRKHTDPVSSDTSPPPRAKGQRVQWNMHKQQSRLSSGSGTSLQNGHSSLSRMNRGEDSGDGDHSHMNGHVEVRRSCRSKNNRFDHLNQSMLFDQLVNSTAEAVLQEMDNISNIRQSREVERLRMWTDTELENMDMYSRVKRRRKSLRRNTFGIQAHREVLSEAEGEEEGTEGDSREFIEVESQEEEEDDEDDEGDEAEEAGEENDRPYNLRQRKTVQRYQAPPIEPARKQRQSTLFDTHRSPARRSHMRVKKHAIHSSDTTSSSDEERFERRKNKSMTRARNRCLPMNLRHEDLASGILRDRVKVGASLADVDPMNLDTSVRFDSVGGLSHHIQALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDRKVSFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIAVIGATNRLDSIDPALRRPGRFDREFLFNLPDKKARKHILKIHTRDWNPKLNDSFVEELAEKCVGYCGADIKALCTETALVALRRRYPQIYASSQKFKLDVSSIVLSPQDFYYAMRNIVPASQRSLMSPGHALSAVIRPLLENTFAEVLAGLHRVFPHAELSQCDKREDLVSELLDDDLDSDDDNDGAPSIFDNPSHSGSPKKQPSANKPFLHFTMSAYHQPTSYRPRLLLAGDRGSGQSTHLAPAALHSLEKFSVHRLDLPTLYSVSAKTPEESCAQVFREARRTVPSVVYMPHVSDWWEAINDTVRTTFLTLLQDVPSFCPVLVLATSETPYAALPEEVKCIFRNSYGEVLNLKRPSEEDRRKFFQDLILVQAARPPAHRKKAGKALEELVPAEPQPPRQLSQEEQKRLEDQEENTLRELRLFLRDVTKRLATDKRFSIFSKPVDIEEVVSDYLEVIRQPMDLSTIMTKIDTYKYLTVKDFLADVDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAMFAAELDPEFNRMCEEIKESRRKRGFQTTAQQTNPPSAAAKRPGIGAEEAARSAQGSAAEGLSTKCNNHIKRKTRRRPPWGRGIIRKKKSYLKKEGEEGGGEPGGEDSEPADCGELDASTEGPEDAVGEEPPDTSLTHDESSCDAMELPAENGEDGHENGHLVSTEEESSNEPPVPSVPGPDNRPKQDESSGKLSEPGLKPTPVKQQDCMNGDESLDSLDSETLSEDKTVETVEGEKKKSAFCEKQEEAKVGSSLSGVPQETTSDLKEGDHENEGTTKMKKCKKTLSEQLKAINVEKQPEDPIEPTPPLVVDHERLSGLLELVTKKSENYTVDQLERLYSLLSQCIYLHRRDYDKTHLTEEMERTVKNFETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJC0</accession>
    <name>W5NJC0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Scavenger receptor class A member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015207736.1">
        <property type="nucleotide sequence ID" value="XM_015352250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJC0"/>
    <dbReference type="FunCoup" id="W5NJC0">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020729"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020765.1">
        <property type="protein sequence ID" value="ENSLOCP00000020729.1"/>
        <property type="gene ID" value="ENSLOCG00000016771.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684245"/>
    <dbReference type="KEGG" id="loc:102684245"/>
    <dbReference type="CTD" id="51435"/>
    <dbReference type="eggNOG" id="ENOG502QV37">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158238"/>
    <dbReference type="HOGENOM" id="CLU_027961_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJC0"/>
    <dbReference type="OMA" id="DLAQECY"/>
    <dbReference type="OrthoDB" id="5835334at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016771">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052376">
        <property type="entry name" value="Oxidative_Scav/Glycosyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39082">
        <property type="entry name" value="PHOSPHOLIPASE C-BETA-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39082:SF1">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="569"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="593" mass="65494" checksum="BE3FFCDAA6B786C1" modified="2014-04-16" version="1">MKERQHSYENQLFKEEDLTGEEEEMQSSRGRSRSGCIKCQQTRSLQLAVKVLYGFFAFLIIAVAVLASLVFRKVDSLSQEESFYNKKIAKVQESIQDLTSTNNCTAWFDVVHFRQEIGKLKKEFEDIQKMILDQEQTLDQASKTQQSLTYSNSRIVRDLQNYSIAIKMINQSLERYLDQVNGWQIVINETEVAMKSFAQDHYDVKATLQQVNSTVALSSLWINAIQRKADEETLVLHKMTAEWQNYSKVLGAMRYNSSTTSQLVRSIQTSISAIYQRLSMSSETVHDMTLHVMNLQMQLDNVTSFMDEHEENMQDLHYHSKYYENRTNERFETLEARMNSLEKEIDTISDSINATVNHVQSMYKYINIESTSCHSKMGSHTEELQNLNNTVLLLLHLSDILRQQYMLLSVRLDLDVRNLSMIMEEMKLVDTKHGQLIKNFTILKGAPGPPGPKGNKGEVGPRGPVGLTGNKGDKGSLGIPGPRGEKGFMGPKGDPGEPGTAGLNGSPGLKGAKGSLGPPGAKGERGLKGDMGPMGQDGAPGPQGPAGIQGAAGLPGIHGFQGIKGKSGPPGPHGPPGPPGPPGPSSGPHLHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJM9</accession>
    <name>W5NJM9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">CUB and Sushi multiple domains 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CSMD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000020874.1">
        <property type="protein sequence ID" value="ENSLOCP00000020838.1"/>
        <property type="gene ID" value="ENSLOCG00000016854.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685076"/>
    <dbReference type="KEGG" id="loc:102685076"/>
    <dbReference type="CTD" id="550553"/>
    <dbReference type="GeneTree" id="ENSGT00940000155701"/>
    <dbReference type="HOGENOM" id="CLU_000277_0_0_1"/>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016854">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000011">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000001">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform X1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000002">
        <property type="entry name" value="CUB and Sushi multiple domains 3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000047">
        <property type="entry name" value="CUB and Sushi multiple domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF3">
        <property type="entry name" value="CUB AND SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0768">Sushi</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869432" evidence="14">
        <location>
            <begin position="24"/>
            <end position="3563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3487"/>
            <end position="3508"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="26"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="137"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="202"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="339"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="403"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="517"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="576"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="687"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="750"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="863"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="922"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="16">
        <location>
            <begin position="1035"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="1096"/>
            <end position="1204"/>
        </location>
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            <begin position="1554"/>
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            <begin position="1731"/>
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            <begin position="2248"/>
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            <begin position="2311"/>
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            <begin position="1380"/>
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            <begin position="545"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS01180"/>
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            <dbReference type="PROSITE" id="PS50923"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000020838.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="3563" mass="389522" checksum="1ED36CCB0203EC4D" modified="2014-04-16" version="1">MTAMRKFKHLLLIFAALTTAANGQTCGGLVQGLNGTIESPGFPHGYPNYANCTWIIVTGERSRIQLSFHTFALEEDFDIVSIYDGQPQPGNLKMRLSGFFLPPPIVSTGSILALWFTTDFAVSAQGFKAIYEVLPSHTCGNPGVIPKGVVHGSRYNIGDKIRYSCVTGNVLEGHAVLTCIVSPGSGASWDFPVPFCRAEGACGGTFRGTSGMISSPHFPSEYENNADCTWSILAEPGDTIALVFTEFQLEDRYDFLEISGTEAPSIWLTGMNLPSPVISSKNWLRLHFTSDSNHRRKGFHAQYQVKKAIELKSRGVKMLPSKDTNHKNTVLSQGGMAADICPDPGIPENGKRIGSDFQVGASIQFSCDDSYVLQGSKSITCQRVTETLAAWSDHRPICRARTCGSNLRGPKGFITSPNYPVQYENNAHCVWVITASDSEKVIKLAFEEFDLERGYDTLTVGDGGKIGDARRVLYVLSGTSVPDLIVSMSNQMWLHLQSDDSIGSQGFKAIYEEIDKGGCGDPGVPAYGKRTGSSFLHGDMLTFECQAAFELVGERTISCQQNNQWSGNKPSCVFSCFFNFTAPSGIILSPNYPDEYGNNMNCVWLIIAEPGSRIHLIFSDFDVEPQFDYLTVKDEGISEATTFGTFSGKDVPSQIASNGHIMRLEFQSDHSNTGRGFNITYTTFGQNECHDPGIPINGQRFGDRFLLGSSVSFSCDEGFIKTQGSESITCVMQDGNVVWNSAVPRCEAPCGGHLTAPAGVLLSPGWPGYYKDSLNCEWVIEARKDHAIKISFERFQTEVNYDTLEIRDGPANSSPLIGEYHGTQAPHFLISTSNHMYLLFTTDNSRSSVGFQIRYESIMMESDSCLDPGIPVNGKRHGNSFAIGSRVTFSCDQGYTLSDEEPLVCERSHQWNHALPSCDALCGGYIHGRSGTVLSPGFPDFYPNSLNCTWTIEVSHGKGVQLFFHTFHLEDSHDYLLITEDGSFTEPVARLTGSVLPPSIKAGLFGTFSAQLRFVSDFSMSYEGFNITFSEYNLEPCDDPGVPAYSRRIGFQFGVGDSLIFSCFPGYRLEGNNKITCLGGGRRVWSAPLPRCVAECGASSVGSEGTLLSPNYPSNYDNNHECIYQIETEKGKGIRLTATNFHLHDGDNLRVYDGKDSSSRLLGNFTKNDMYGVIINSTSNHLWLEFNSNGSGTDRGFRLTHSSFDLVKCEEPGTPNYGYKIQDEGHFADTFVVYSCNPGYTMHGSSVLTCLSGDRRVWDKPLPSCIAECGGHITGATSGRILSPGYPAPYDNNLHCTWSIEADSGKTISLHFIVFDTEVDHDILKVWDGPREGGILLKEWSGSSLPEDTHSTFNVLTLQFDSDYFISKSGFSIQFSTTTATTCNDPGTPQNGSRYGDSREPGDTITFQCDPGYQIQGAAQITCVQLNNRFFWQPDPPTCIATCGGNVTGPAGVILSPNYPQPYPPGKECDWRIKVNPDFVIALIFKSFNMEPSYDFLHIYEGEDSNGPLIGSFQGSQAPDRIESSGNSLFLAFRSDASLGMSGFAIEYKEKPREACFDPGNIMNGTRIGTDFKLGSTVTYQCDSGYTTVGPSTITCIIGSDGKPVWERILPTCKAPCGGQYTGSEGVVLSPNYPHNYTAGQTCLYSITVSGEFVVFGQFAYFQTAMNDSVELFDGASQNARLLSSLAGTHTGETLPLATSNQILLRFSAKSGASARGFHFVYQAVPRTSDTQCSSVPEPRYGRRIGSEFSAGSIVRFECNPGYFLQGSKAIKCYAVPNALAQWNDTIPTCVVPCSGNLTERRGTILSPGFPEPYGNSLNCVWRITVTEGAGIQIQVISFATEHNWDSLEIYDGGDMAAPKLGSFSGTTAPALLNSTSNQLYLHFHTDISVVAAGFHLEYKTVGLTTCPEPLVPANGIKTGDRFMVNDVVSFSCEPGYVLQGHSHISCMPGTVRRWNYPPPLCIARCGGTLTAMTDVILSPGFPGNYPGNLDCTWKILLPVGHGAHIQFVHFSTEDNHDFLEIRNGPHHTSSLIGQFSGNQLPPALLSTTHETVIHFYSDHSENRQGFKVSYQAYELQSCQDPSPFHNGYIINSDYNAGQSITFECYPGYVLIGHPVLTCQHGVNRRWNRPFPRCEAPCGYNVTAQNGTIYSPGYPNEYPNSQDCTWLITVPQGHGIYINFTLLQTEPVTDYIAVWDGPEENYPQLGVFSGNTALESAYSSFNQVLIRFHSDFSTSGFFVLNFHAYRLKKCPAPPSVEEAEFFSEDQDYEIGDIVKYQCFPGFTLVGSEELTCKLNSHLQFEGPPPSCEAQCPLNEERTASSGVILSPGYPKTYPNSQTCSWTIKVQTGYTISIYIEMFQSEKQFDELEIFDGPAGESPLLVALSGNHTTQLNFTSKTNQLYLRWSTDHATSKKGFKIRYTASYCSVNSVPRNGGVFNRTQGNAGSRLYYFCNAGYRLVGQSNTTCKLHQNGLYHWDSPVPLCQAVSCGIPGPPGNGTFHGNQYTVGSRVHYQCNEGYRLESTRSSTAVCQEEGTWSNRGQPPQCLPVECPNIETILSEHMVWRLISGSQNEYGAQIMLSCTPGYFLGGRRIVQCLANGTWSGSEEKAACKIISCGELPSPPNGNKIGTLSTFGATAIFTCNTGYTLAGSHVRECGANGLWTSSETRCLAGHCGSPEPITNGHISGDGFSYRDTVVYQCNLGFRLIGTSVRICQQDHKWSGKAPVCVPITCGHPGNPANGRTNGSEFNLNDIVNFTCNTGYLLHGAPRAQCRVNGQWNSALPMCRVVNCSDPGFVENAIRHPQQHYPESFSYRTSIMYHCKKGFYLLGSSVLSCQSNGFWDRSLPKCLPISCGDPGTPPNAVLSGKIFSNGAVVHYSCSRGRTLIGNATRVCQEDGRWSGSPPYCSGDSPGFCGDPGIPPHGSRLGDEFRTKSLLRFTCEAGYTLIGSAERTCLPNGSWSGAHPVCEAVSCGNPGTPAFGRIVHSDGILFSSSVTYSCWEGYKTSGLTTRHCTTNGTWTGSAPDCTVISCGDPGPIANGIYIGNEYAYNKTVHYHCNPGFIMEPAGSSALLCTKDGTWNQTKPTCRALSCGQPPAIHHGKVEGSDFQWGSSISYSCFEGYQLSTPAIVACEGTGTWRGDVPHCLPVLCGDPGTPAEGHAEGKSFTYRSEVTFYCKGPYILVGSSRRVCQADGTWSGIQPTCIDPAYNMCRDPGTPPFGIPIPSQGYEVGSKIFFKCRKNYHILGSTTRTCLENLTWSGIQPECISHACRQPETPSHVDVKAIDLPTLGYTLMYTCQAGFFLAGGSEHRTCKADGKWSGKPPICKVGPKVNDKTKGADSGVQKNKIHVPADVFSLNSGWKGFYEYLGRRQSATITVNWFNATTSKVNVTLLEHSGVQLRLSGIYKKEENHLLLKVYQVRGPIEIFFSKFKNDNWAMDGYVSAESDSNTFVYQGFIHGKDFGNFQLTRQGLITTDTDLSNPYYGTNSSSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENTNGQASFENPMYDTNMKPTEAKAVRFDTTLNTVCTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK78</accession>
    <name>W5NK78_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibulin C-terminal Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK78"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021037"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021073.1">
        <property type="protein sequence ID" value="ENSLOCP00000021037.1"/>
        <property type="gene ID" value="ENSLOCG00000017021.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156642"/>
    <dbReference type="HOGENOM" id="CLU_1839608_0_0_1"/>
    <dbReference type="InParanoid" id="W5NK78"/>
    <dbReference type="OMA" id="PNDANCV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017021">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibulin C-terminal Ig-like" evidence="2">
        <location>
            <begin position="29"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="141" mass="16205" checksum="F0D6E2EC9ADA567B" modified="2014-04-16" version="1">RIRMDRTETIRCVKSCMPRDVDCILDTVHTISYTVISLPTFRDFARPEEIVFLRAVSPAYPSVQTSTPDIVFDIIEGNLHNSFDIIKRYENGMTVGVVRQVKPIIGPLHIVLKLGMNYVVRGVISHRNVVNVHIYVSEFWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK89</accession>
    <name>W5NK89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK89"/>
    <dbReference type="FunCoup" id="W5NK89">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000021084.1">
        <property type="protein sequence ID" value="ENSLOCP00000021048.1"/>
        <property type="gene ID" value="ENSLOCG00000017031.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271932"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_0_1"/>
    <dbReference type="InParanoid" id="W5NK89"/>
    <dbReference type="OMA" id="YVVMYIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="451" mass="51459" checksum="2ABAA2FB1AD23DF1" modified="2014-04-16" version="1">CSMEQSEIQAVGYCFESNSSCLKEVRSTAVYVVMYIFTVAVVMLTVCGNLVVIISISHFKQLHTPTNFLMLSLAVTDFLVGAVVMPFSIILLIETCWYFGEMFCIIYKILFFFLISVSIGNVAYIAIDRYFVVCYPLLYSTKVTVNVTAAVILLNWLISLLYNLAFINMGGTEMLNFCLGDCLLFITKSWVIIDLLCTLILPCSIMITLYFKIFVVARKHAHAISSVTEKKKLEHCFCAWSLSLMSEPHFLIKYYFSTLISIQHYKIHYFVTVHYDVRSAKGCSLSFIKINKLTSNPVSSRRRSQQPLCYNCHTTIKEFPGTAKQTKDSRIDSVQWNTNSSCLKEVRSTAVYVVMYIFTVAVVMPDGDNNKISTKSERKAAKTLGILVIVFLLCLVPYYICSLALENVDRTSLLFSLLSWLLYFNSAINPIIYALFYPWFQKSDFFLLFQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKS0</accession>
    <name>W5NKS0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">2P domain potassium channel Talk-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">TWIK-related alkaline pH-activated K(+) channel 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="23">Homodimer; disulfide-linked. Heterodimer with KCNK17 and KCNK5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKS0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021229"/>
    <dbReference type="GlyCosmos" id="W5NKS0">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000021265.1">
        <property type="protein sequence ID" value="ENSLOCP00000021229.1"/>
        <property type="gene ID" value="ENSLOCG00000017176.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159813"/>
    <dbReference type="HOGENOM" id="CLU_022504_1_0_1"/>
    <dbReference type="InParanoid" id="W5NKS0"/>
    <dbReference type="OMA" id="WLALIFN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017176">
        <property type="expression patterns" value="Expressed in zone of skin and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000098">
        <property type="entry name" value="Potassium two pore domain channel subfamily K member 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF104">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19 27" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15 27" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="7" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 27 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 27" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="29">
        <location>
            <begin position="93"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="29">
        <location>
            <begin position="187"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="26">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00065989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR038061-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="29487" checksum="8A171EC20FC94FA5" modified="2014-04-16" version="1">TLRWGIMARFHANMRVSWTALLVLGYFLYLLVGATIFQILERAAESQNRNHFQLEKLTFLKNYSCLDGPALERFVQVILDAWEKGVNPSGNSTNPSNWDFSSSFFFAGTVITTIGYGNLSPSTVSGQVFCVFYALCGIPLNLAFLNQLGKGLTAHLGRLERSVLTAGRHEQTVKVLAMSSFLVMGTLLFLVFPPVIFSYVEGWSYGEGFYYAFITLSTIGFGDYVVGTDPDKHYISIYRSLAGVWIIFGLAWLALLFNLGASVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL53</accession>
    <name>W5NL53_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PEX7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL53"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021362"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021399.1">
        <property type="protein sequence ID" value="ENSLOCP00000021362.1"/>
        <property type="gene ID" value="ENSLOCG00000017270.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157264"/>
    <dbReference type="HOGENOM" id="CLU_046581_2_1_1"/>
    <dbReference type="InParanoid" id="W5NL53"/>
    <dbReference type="OMA" id="CECNENL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017270">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059104">
        <property type="entry name" value="Beta-prop_EIPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23609">
        <property type="entry name" value="Beta-prop_EIPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EIPR1-like beta-propeller" evidence="12">
        <location>
            <begin position="43"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="91"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="135"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="24550" checksum="36974148FDD67FC9" modified="2014-04-16" version="1">LNVYESLCVQVYSVDWSLTRGETFIVSGSWDHTAKVWDPEIAKSVGTFQGHESVIYSTSWSPHIPGCFASASGDSTLRIWDLKTGGSRLVIPAHQAEILSCDWCKYDQNLIVTGAVDCSLRVWDLRNARQPLSELTGHTYAVRRVKFSPFQLTMLASCSYDFTVRFWDFSKTESLLETVEHHTEFVCGLDFNLHIPNQVVDCAWDETIKIYTPPCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLF8</accession>
    <name>W5NLF8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G protein-coupled receptor 75</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638834.1">
        <property type="nucleotide sequence ID" value="XM_006638771.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLF8"/>
    <dbReference type="FunCoup" id="W5NLF8">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021467"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021504.1">
        <property type="protein sequence ID" value="ENSLOCP00000021467.1"/>
        <property type="gene ID" value="ENSLOCG00000017362.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686571"/>
    <dbReference type="KEGG" id="loc:102686571"/>
    <dbReference type="CTD" id="10936"/>
    <dbReference type="eggNOG" id="ENOG502QVED">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007723"/>
    <dbReference type="HOGENOM" id="CLU_041999_0_0_1"/>
    <dbReference type="InParanoid" id="W5NLF8"/>
    <dbReference type="OMA" id="PFMGAPV"/>
    <dbReference type="OrthoDB" id="8732677at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017362">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15007">
        <property type="entry name" value="7tmA_GPR75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF55">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 75-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="55"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="533" mass="59120" checksum="A33A8D7B18A20553" modified="2014-04-16" version="1">MNISTPSMQLAEESLQETYNGTGNLVLPSGALIHTATLVTCSFLLVIIFCLGSYGNLVVFLSFFDPAFRKFRTNFDFMILNLSFCDLFICCVTAPMFAFVLFLDSGSGVSKEFCFTFHLTSSGFIIMSLKTVAVIALHRLRMVLGQQPNRTASFPCTLMLTVLLWTTSFTLATLVTLRTYPWKSRVCLPHFGLIGGDGKIILYLYLVDFAFCVGIVSVSYVMIAQALRKNAQVRKCPIITVDASRPQPLIATGFEGMQCTVPALYRNQTYNKLQHVQTHPYTKKMSQTGTGGTKFQLVSSVNLATAKDSKAVVTCVVIVLSVLLCCLPMGISLVQDVLSPESSFILYQFELCGFTLIFLKSGINPFIYSRNSAGLRRRVLWCLQYVALGFLCCKQKTRLRAMGKGSLEVNRNKSSHHETNSAYMLSPKHQRKLVDQACGPSHSRDSILSPKATGGHQPHAQSSSTPINTRIEPYYSIYNSSPSQEISSPNSLQPVNSTFGFAKSYVAMHYHTRKDMLQDYESTSARQIPMPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLT3</accession>
    <name>W5NLT3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cysteinyl leukotriene receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016891">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLT3"/>
    <dbReference type="FunCoup" id="W5NLT3">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021592"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021629.1">
        <property type="protein sequence ID" value="ENSLOCP00000021592.1"/>
        <property type="gene ID" value="ENSLOCG00000017487.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUJU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286937"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NLT3"/>
    <dbReference type="OMA" id="FMPYHVQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017487">
        <property type="expression patterns" value="Expressed in mesonephros and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15158">
        <property type="entry name" value="7tmA_CysLTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013310">
        <property type="entry name" value="CLT1_recept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004071">
        <property type="entry name" value="Cyst_leuk_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF45">
        <property type="entry name" value="CYSTEINYL LEUKOTRIENE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01902">
        <property type="entry name" value="CYSLT1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01533">
        <property type="entry name" value="CYSLTRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="52"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="354" mass="40501" checksum="9DC7B588A095DC9E" modified="2014-04-16" version="1">FSLSCPRVSLSRCNMLVNHSLSNNTQCPSIDDFRNQVYSTVYSIITVFGFVGNGFALFVLIRTFRQRSAFHIYMLNLAVSDLLCVSTLPLRVIYYVNKGQWNYGDFLCRISSYALYVNLYCSIFFMMAMSCTRFIAIVFPVQNLKLVNERKAKIVCVCIWVFICATSSPFLITGIHIDPLTNKTKCFEPPEKRKSISKLIILNYFSLVVGFAVPFLVILICYIGIIRTLLTGSSSMKKQKSTRTKAIRMIVIVMLAFLISFMPYHIQRTVHLDFMSKPDRTCDDVVYMQKSVVITLCLAAANSCFDPMLYFFSGENFRRRLSTFRKASMSVGPNVRRQKLDVSKSQQDEDEALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNX7</accession>
    <name>W5NNX7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 518A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Through its association with the EHMT1-EHMT2/G9A and PRC2/EED-EZH2 histone methyltransferase complexes may function in gene silencing, regulating repressive post-translational methylation of histone tails at promoters of target genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022336"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022377.1">
        <property type="protein sequence ID" value="ENSLOCP00000022336.1"/>
        <property type="gene ID" value="ENSLOCG00000018235.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162006"/>
    <dbReference type="HOGENOM" id="CLU_005711_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNX7"/>
    <dbReference type="OMA" id="SCVEECM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018235">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001423">
        <property type="entry name" value="Zinc finger protein 518A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1294"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="436"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1123"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1144"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1328" mass="145149" checksum="CE8BB4CD1181252B" modified="2014-04-16" version="1">CYQFRQSPQDGVKISGKILRFSCSECKGEAAFSPNDLLKHFQVAHQGCLPTYPCDMCNFTANDFPVLQQHRLGHRDTYVKCEICNDNVQYSLLQLTRHFSSYHSSNGHYRCEKCKFSTKDVGTFVQHIHRHSEVQYKCGKCQHVSYTKGEFQRHLLVHTGTFPFQCQFCDYGATRKDYVLKHMNAVHSDEIERKCKLKDKEDGAKTLADAKSGLKLLLKRNTAGVARDAQWRTAGFHALSGGGLLDEYGRLANPEKTLEETQQFLERTVASEKDCIQWAKARKSEQQYLSPSTPAVLKPKMESCLGSNSGFLKTGGSGSSVLMVKNKISIPPNCTTKFMGFKMVDGKRHLVLKVIPAAKLGSSPQTVAPSVEKDAKGRGPEKGMNGPICVEDVALQCSSLPVSSHSSLSQSTLPACSDSQSSINKGSSVVKELLQAENQESSSTSHTVVNDGHPISDDEQKPSSSASPTTCKVFHTTSQKAQIAHPSKAEMDQQSEHTSLVEFASNKTSPEMDKESLSSKETLLESIVVDNENNSDLNSSRQEVFSFHNYSKDRASSESSDTVKESGDQEASQLMTDVCVKDSFQSQCAKDLTGTSLTLADVLSPHSQDCKVDEEMGEIIADQLSSQGVMEKVPDSEIEVDECIATVEEDSAEPSDVLEEPEDSLFMPKITSVFSLQSEENGPQLDVNQLLEKVLETENKKASILSAQEKKELKVAAGESTVGNPCSATALGRILEEHSDAIISQQLEKERISSANQDPVKNSAATLRILQPINVTEGKKQVFLKPCQNGFALPIQVGSNQGFQLITGSSHPQINVSYLNTGKERPKKPPGLTFTLNNGRIGTTAQIVGDKKVSGGAGNGEESAVLCCGSSTGHYIVNNMPLKGSLILSNSVQSLSGERTSNLPTCFLVQRQLPMVTSTSCQSGVTVSHNIQGQPQRPVLAVPINSPEKPAVVQAGRQAFLLKCVTPVKQGTLLNSQIEAKVLNQNCTSNESGGNKVLLKIVRNASGSSFPSSTGELNGIQTLAMTNQPVYLTTGALQSSCISRSSNQSIVNVSTGYKTSGPSQDSSNEPLTVSQLLNCSSPAAVQGKSKEKESGVNSHGKIKRVCLPSPKQNHLAIRKKRQRGLLLEESSGPSPKAKKPLSKKWKETEKGPADAHELCATPVPKDVERTLRLSPFSATQRIKCPRRNQPVVVLNHPDADVPEVVNVMKTINKFKGHVLKVALSQRTIDALSELNCDPLNENPSLNIHATRSRRAKPVSPVKERYILKLRLKKTSRNKYKVVKSTSDSTEKPTFSCWFCGRIFDNQEEWIGHGQRHLMEATRDWNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-03-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q8HQN1</accession>
    <name>Q8HQN1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 25">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="6 25">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">COI</name>
    </gene>
    <organism evidence="29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="29 30"/>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2003" name="Mol. Phylogenet. Evol." volume="26" first="110" last="120">
            <title>Basal actinopterygian relationships: a mitogenomic perspective on the phylogeny of the 'ancient fish.'.</title>
            <authorList>
                <person name="Inoue J."/>
                <person name="Miya M."/>
                <person name="Tsukamoto K."/>
                <person name="Nishida M."/>
            </authorList>
            <dbReference type="PubMed" id="12470943"/>
            <dbReference type="DOI" id="10.1016/S1055-7903(02)00331-7"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2003-10" db="EMBL/GenBank/DDBJ databases">
            <title>Molecular Evidence for Permian teleosts.</title>
            <authorList>
                <person name="Dunn K.A."/>
                <person name="Yang Z."/>
                <person name="Coates M."/>
                <person name="Thomas M.G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 25">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 25">Mitochondrion inner membrane</location>
            <topology evidence="3 25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="6 25"/>
    <dbReference type="EMBL" id="AY442350">
        <property type="protein sequence ID" value="AAR16131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB042861">
        <property type="protein sequence ID" value="BAB40731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8HQN1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000025123.1">
        <property type="protein sequence ID" value="ENSLOCP00000022473.1"/>
        <property type="gene ID" value="ENSLOCG00000020981.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:807203"/>
    <dbReference type="GeneTree" id="ENSGT00390000001518"/>
    <dbReference type="HOGENOM" id="CLU_011899_7_3_1"/>
    <dbReference type="OMA" id="WAMMSIG"/>
    <dbReference type="OrthoDB" id="10002679at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000020981">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051597">
        <property type="term" value="P:response to methylmercury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01663">
        <property type="entry name" value="Cyt_c_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033944">
        <property type="entry name" value="Cyt_c_oxase_su1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="20 25" id="KW-0186">Copper</keyword>
    <keyword evidence="17 25" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 25" id="KW-0349">Heme</keyword>
    <keyword evidence="19 25" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="23 25 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22 25 29" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 25" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 25" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="21" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-1278">Translocase</keyword>
    <keyword evidence="11 25 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 25" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="27">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="AAR16131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="BAB40731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="518" mass="57177" checksum="FBA1C03FC8196F32" modified="2003-03-01" version="1">MAITRWFFSTNHKDIGTLYMVFGAWAGMVGTALSLLIRAELSQPGTLLGDDQIYNVIVTAHAFVMIFFMVMPVMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSGIEAGAGTGWTVYPPLASNLAHAGASVDLTIFSLHLAGISSILGAINFITTILNMKPPAASQYQTPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDPAGGGDPILYQHLFWFFGHPEVYILILPGFGMISHIVAYYAGKKEPFGYMGMVWAMMAIGLLGFIVWAHHMFTVGMDVDTRAYFTSATMIIAIPTGVKVFSWLATLHGGSIKWDTPLLWALGFIFLFTVGGLTGIVLANSSLDIMLHDTYYVVAHFHYVLSMGAVFAIMGAFVHWFPLFTGYTLHNTWSKIHFGVMFAGVNLTFFPQHFLGLAGMPRRYSDYPDAYALWNTISSIGSLISLVAVIMFLFIVWEAFSAKREVLSVELTSTNVEWLHECPPPYHTYEEPAFVQVQVTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVS2</accession>
    <name>W5LVS2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVS2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000229.1">
        <property type="protein sequence ID" value="ENSLOCP00000000229.1"/>
        <property type="gene ID" value="ENSLOCG00000000206.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217173_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVS2"/>
    <dbReference type="OMA" id="CIYSVCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000206">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="228" mass="24661" checksum="5EB3480FEF3B6BC7" modified="2014-04-16" version="1">VYCVYSVCIYSVYSVCECTVCLYTVCTLCVCTVCIYSVCTVCTVRVYCVCIYSVCTVCVQCECTVCVYTVCVQCVYIQCVYSVCVYNLCVLCGQCVYIQYVQCVCTVCVCVQCVCTVCVYSVCIQCVYSVYIYRISCTCVVAMEILKILHTLALPSASPLATSSCHSTCADIIAAGSPAPTEPQWLIGQHAGAPPIRPEQAGHHHHCQGHLLSMPKAHRGTGGDCAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVV5</accession>
    <name>W5LVV5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVV5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000262.1">
        <property type="protein sequence ID" value="ENSLOCP00000000262.1"/>
        <property type="gene ID" value="ENSLOCG00000000238.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SPVS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164014"/>
    <dbReference type="HOGENOM" id="CLU_009579_23_0_1"/>
    <dbReference type="InParanoid" id="W5LVV5"/>
    <dbReference type="OMA" id="LQITITL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000238">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000892">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF85">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="64"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="33474" checksum="FC31215A88B4E7A2" modified="2014-04-16" version="1">IFGSAPHLNSSLTHSPVTGSAGPMQAPLLQLLRDLQYFLDSVYQEDWSSGTVTNLFVFCVGLPVNCLAIYGLCRLVKSGMAVPVYVINLLVSDLLQLAVKPVLIARRLSPCPGLQLQRAYAASKMIHFFGLCGSIWFMVCIALERYLVVGRPLWYRSHRGLWRTGLVSLAVWAFTLAVGGLYVLGHLVVTVSEGAELLFLLIPYFLPFPLLVSSCAATGRALSGPGAGVVSARERRRVLQTLGLVLGGYAVLFLPRYLARWLLWAKGDSRPAQLFYRVTGELVGLNPLLDPVLYILVRKDVSSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW81</accession>
    <name>W5LW81_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="W5LW81"/>
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        <property type="protein sequence ID" value="ENSLOCP00000000388.1"/>
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            <end position="87"/>
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    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="128"/>
            <end position="224"/>
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    </feature>
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            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="24503" checksum="16EE8778EDCABE44" modified="2014-04-16" version="1">ETLQLEKGMVSESPVGSSVVISCTTSNKQWFASGYSALHWYLQKPGQAPQLLVYAATNKCSGSGSGTAFTLTITGVQAEDAGDYYCQQDYNFPFTQCVWYILFRMYVSSAIKIQMYSRHTVAGTEGQITMTLTPATVSVSPEQDVSLQCSASQGISWSLTWYQLKPGHSPRLLIKAGDERFHGVPTHFGGSRSGTVFTFKITGVQAEDAGDYYCQQYDSTQPSS</sequence>
</entry>
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        <recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="W5LW85"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="match status" value="1"/>
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            <begin position="28"/>
            <end position="150"/>
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    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS50835"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000000392.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="187" mass="20339" checksum="4083090578086DC9" modified="2014-04-16" version="1">MGVYRCVVTVGGQDTVSLEGHIELEGLPHFSEEPQDKTVAANHSFSLVCRAHGPPEPVHVIWLQDGAPLNKLDQPLALSPSTLTLSGTVSLSPSVCMFIFFSFSVWQADGMMASISCYLYISISLYHCQYSSVPVCHCVSAAVYQYITVSVHQCASISLCQYSSVPVSHWSVQQCASMSLCEWISVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWM0</accession>
    <name>W5LWM0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FIIND domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWM0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000527"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000528.1">
        <property type="protein sequence ID" value="ENSLOCP00000000527.1"/>
        <property type="gene ID" value="ENSLOCG00000000480.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3573">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111912"/>
    <dbReference type="HOGENOM" id="CLU_736809_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWM0"/>
    <dbReference type="OMA" id="PECASFE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:NLRP3 inflammasome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140608">
        <property type="term" value="F:cysteine-type endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Death Domain, Fas"/>
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        <property type="entry name" value="FIIND_dom"/>
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        <property type="entry name" value="NLRP_Inflammasome"/>
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    <dbReference type="PANTHER" id="PTHR46985:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23679">
        <property type="entry name" value="UPA-FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51830">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIIND" evidence="8">
        <location>
            <begin position="12"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="7">
        <location>
            <begin position="288"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000527.1"/>
        </source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42267" checksum="7685CF8BFDDECD6F" modified="2014-04-16" version="1">MSEDDTVKTPPVLPHSSFRPQTRPDGSRVRCSFAFSSRGCYACSITGLRFVMALPAEVQYWTEPFRAEPSLLSSLQPVSPTYNINCPVSEALRLVCFPHSETSPVTDIWAQLKVAHRLDGNTEIIQPTRVTDSHVEVKVDKLSPFTIVLKILGFKVEVRGQLLLFLLKLSSRQKLIILLLPSNVNVEEVKSKYTGYKFIPTFSNCVLLESTEYSLSCGIEGSTVQPKKHLFFRDYGPNFHGTFEVILSQEVKELELELFRSKSKELRVWSGKVLLEDAVEVAAPPAARDQAGGHFVDRHFTELVSRVTVVDPILDSLLQKNLITLEGYIKIRSRDTPSEKMRELLLGPMVSSGDRGKDELLRILAKEFPCLMADLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWV4</accession>
    <name>W5LWV4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nectin cell adhesion molecule 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. Acts by forming homophilic or heterophilic trans-dimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Cis- and trans-homodimer. Can form trans-heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWV4"/>
    <dbReference type="FunCoup" id="W5LWV4">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000611"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000612.1">
        <property type="protein sequence ID" value="ENSLOCP00000000611.1"/>
        <property type="gene ID" value="ENSLOCG00000000554.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWSY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165364"/>
    <dbReference type="HOGENOM" id="CLU_029618_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWV4"/>
    <dbReference type="OMA" id="CHFATFP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000554">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098631">
        <property type="term" value="F:cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001618">
        <property type="term" value="F:virus receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042267">
        <property type="term" value="P:natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000298">
        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052659">
        <property type="entry name" value="Nectin/PVR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47387">
        <property type="entry name" value="NECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47387:SF1">
        <property type="entry name" value="NECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="117"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="315" mass="33866" checksum="8E0A754F458E796F" modified="2014-04-16" version="1">QHVKVEPEVLAYPGQDVTLRCQFVQDGEAKLTQVSWIWEPVNGQKQNIAVFHPNFGASYPSSPLKGRISFINSSLENPSIHITALVLGDEGKYTCEYATYPSGNDQGTTSLFVLVKPRSSADIITVPAGDSPVAVARCVAAGGRPAADVSWETAAQGNWTTVPTQNSDGTVTMTSEYLLAPTSEDNGKDISCVVKHKTLKIPARFPLKLFVEYRLTLSDTQYSPYWASHLLQHCLTGQMPENVVISHNKLTVLKVDEAVNSTFVCEVNTRLGATREQISVIVRGPSLTPSLSGQDGRGVILSAALGSAPLGALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYZ9</accession>
    <name>W5LYZ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYZ9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001356"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001361.1">
        <property type="protein sequence ID" value="ENSLOCP00000001356.1"/>
        <property type="gene ID" value="ENSLOCG00000001206.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000265001"/>
    <dbReference type="HOGENOM" id="CLU_1532030_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYZ9"/>
    <dbReference type="OMA" id="ITPCRER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001206">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="175" mass="20335" checksum="6FECA5F4793488FA" modified="2014-04-16" version="1">MTQMVCAVSITPCRERGVNTGLQQPETGGGMRDRQADKREREKETGRPTDKWERDRQEREKRTDRRERERQADRQAGRRERNGQTGGREREERDRQTGERERQAGERETDRQAGERERQAGERETDRQAGERERQAGERETDRQAGERDRETGQRRGEGQVAALPASSLTSRTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ99</accession>
    <name>W5LZ99_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA-queuosine alpha-mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.110</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glycosyltransferase-like domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Glycosyltransferase that specifically catalyzes mannosylation of cytoplasmic tRNA(Asp) modified with queuosine at position 34 (queuosine(34)). Mannosylates the cyclopentene moiety of queuosine(34) in tRNA(Asp) to form mannosyl-queuosine(34). Mannosylation of queuosine(34) in tRNA(Asp) is required to slow-down elongation at cognate codons, GAC and GAU, thereby regulating protein translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>queuosine(34) in tRNA(Asp) + GDP-alpha-D-mannose = O-4''-alpha-D-mannosylqueuosine(34) in tRNA(Asp) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12885"/>
            <dbReference type="Rhea" id="RHEA-COMP:18572"/>
            <dbReference type="Rhea" id="RHEA-COMP:18581"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="ChEBI" id="CHEBI:194442"/>
            <dbReference type="EC" id="2.4.1.110"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:12886"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.110" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01011660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636384.1">
        <property type="nucleotide sequence ID" value="XM_006636321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ99"/>
    <dbReference type="FunCoup" id="W5LZ99">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001456"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001461.1">
        <property type="protein sequence ID" value="ENSLOCP00000001456.1"/>
        <property type="gene ID" value="ENSLOCG00000001286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685820"/>
    <dbReference type="KEGG" id="loc:102685820"/>
    <dbReference type="CTD" id="227835"/>
    <dbReference type="eggNOG" id="ENOG502QQJ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006631"/>
    <dbReference type="HOGENOM" id="CLU_033439_1_0_1"/>
    <dbReference type="InParanoid" id="W5LZ99"/>
    <dbReference type="OMA" id="HRWEYDK"/>
    <dbReference type="OrthoDB" id="10032790at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001286">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016438">
        <property type="term" value="F:tRNA-queuosine(34) beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051862">
        <property type="entry name" value="GT-like_domain_containing_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022701">
        <property type="entry name" value="QTMAN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615:SF3">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12038">
        <property type="entry name" value="QTMAN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-queuosine alpha-mannosyltransferase N-terminal" evidence="11">
        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="10">
        <location>
            <begin position="291"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="205"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="53091" checksum="22E93F8D0A56AE57" modified="2014-04-16" version="1">MSVLLIEAFYGGSHKQLIDLLQENVKDCVTYSLPAKKWHWRARTAALYFMQAVPHSVTYRVLLTSSVLNLAELTALRPDLAGLKKVLYFHENQLVYPVRKSQERDFQYGYNQVLSCLVADVVVFNSAFNMESFLTSIPTFMKLIPDHRPKDLEKLIRPKCQVLYFPIKFPDVRRFLPEHKLNFYKRRLDFEERRYPGLSGSFSGSKHCTGTELPTDNSTSDLTPEPREKVCTTEQEALNPPEPPLETGRADEAAIPHHADDKQQPSLEPGGTGVPEQRCVGPATQQRPLHIVWPHRWEHDKDPEVFFKTLLKLKERGLSFQVSVLGETFTDVPDIFAEARKDLDGYVSHWGYIPSKDDYLKALCEADVVVSTARHEFFGVAMIEAVYCGCYPLCPNALVYPEIFSAEYLYSTPEQLFKRLQDFCRRPDRARGYHAKVDTDQFSWAALCKKFRSLLAAEAREDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZF1</accession>
    <name>W5LZF1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01034115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZF1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001508"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001513.1">
        <property type="protein sequence ID" value="ENSLOCP00000001508.1"/>
        <property type="gene ID" value="ENSLOCG00000001327.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161486"/>
    <dbReference type="HOGENOM" id="CLU_1431117_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZF1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001327">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000114">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000892">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF19">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21101" checksum="7B852A389103EA9F" modified="2014-04-16" version="1">EADPAKVKVEAIVISDEELEEMEGMFQRRGRAEEEEEEYVEGLGAGEEEEEVLAMHMGNPPFLPSHHLLHAHLPHAHAEALTFHPALVSPPSPTSGPGAPGGPPSFPGLLAPDPLGPYVEDVPTCATCGKTFSCAYTLRRHAIVHTRERPYECRYCYRSYTQSGDLYRHIRKAHNQDLPLKRSRTDSEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZG2</accession>
    <name>W5LZG2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PDZ domain containing ring finger 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LZG2"/>
    <dbReference type="FunCoup" id="W5LZG2">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001519"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001524.1">
        <property type="protein sequence ID" value="ENSLOCP00000001519.1"/>
        <property type="gene ID" value="ENSLOCG00000001340.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689360"/>
    <dbReference type="KEGG" id="loc:102689360"/>
    <dbReference type="CTD" id="29951"/>
    <dbReference type="eggNOG" id="KOG0312">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183062"/>
    <dbReference type="HOGENOM" id="CLU_011096_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZG2"/>
    <dbReference type="OMA" id="TMEQDYY"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001340">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000028">
        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF6">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="143"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="522"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="394"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="778" mass="89273" checksum="DC1A1C41809E35E1" modified="2014-04-16" version="1">MGCNLCTLQKREDHYKLLYEIAQVNGKDLSKATHEEAVEAFRSAKEPIVVQVLRRSPISKSHGNSQEVQLVDVCTQTDITFEHIMALSKLRPSSPPVPDICPFLLSDSCHSLHPMEHEYYEGTEFLSNMPVDGDRADEFEYEEVELCRMNSQEKLGLTLCYRTDDEEDIGIYVSEVGPNSIAAIDGRIREGDRILQINGCDVQNREEAVALLSSEDCRNIVLLVARPDIQLDEGWIDDEHTEFLEELKMEMLEDQHKDIVQYPSNELQEPKKTEEDDGTTDTATTSSNNQEKDSGVGRTDDSVRNDESSEHENLADVQQQASVKDKQEMEQSQDMLGSNDIQCIESFASGEHIDSDCIRAQEEDCDRFRQLLELKCKIRNSGEYDLYYSRSSTIECSLKEQDGVERELRLLNEELRSIELECQNIMQAHKLQKARDQYSGMWSVKDESFHNFNTSMDLQGSKLAEINEHPEKSDKDSSSAYNTAESCRSTPLAMDRSPDHSLQRMVSITNRKNLRSSMTTCQMTSSQGNKGAFLGKAKTSGQNSTTESDEIVPDDKKRSEQERIGLQQIPYLSPCHSSQYRHANIPAHARHYQSYMHLIQQRSAVEYAQSQLSLVSMCKDLQKSSEPKMEWKVKIRSDGTRYITKRPVRDKILKERALKIKEERSGMTTDDDMMSEMKMGKYWSKEERKQHLVRAKEQRRRREFMMRSRLECLKESPQSGSEGKKEINIIELSHKKMMKKRNKKILDNWMTIQELMTHGAKSPEGTKVHNAFLSVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0G8</accession>
    <name>W5M0G8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBX domain-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">UBX domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in endoplasmic reticulum-associated protein degradation (ERAD). Acts as a platform to recruit both UBQLN1 and VCP to the ER during ERAD.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Directly interacts with VCP. Interacts with UBQLN1. Forms a complex with VCP and UBQLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0G8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001875"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001880.1">
        <property type="protein sequence ID" value="ENSLOCP00000001875.1"/>
        <property type="gene ID" value="ENSLOCG00000001630.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160205"/>
    <dbReference type="HOGENOM" id="CLU_039222_1_1_1"/>
    <dbReference type="InParanoid" id="W5M0G8"/>
    <dbReference type="OMA" id="FEPNNTS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001630">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16117">
        <property type="entry name" value="UBX_UBXN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23187">
        <property type="entry name" value="UBX7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="UBX" evidence="8">
        <location>
            <begin position="315"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="54127" checksum="E0C7B11386D43D64" modified="2014-04-16" version="1">MLWFEGSIPAAIASAKLQNSVFVVVITGDDEQSTQVISSWEDGRVTEATGQSFVAIKIDAKSETCVQFSEIYPVVCIPSSFFIGDNGIPLEVIAGSVPAAELVKRIEKVKQVLLSPFLAGQMHTQRQGGHGAGPTPPPSVAPETATPQEPISGPSGAAELAEPRPLAAAADGPAAPAASKGELAQAVGTVKQEEPSATLPETVFKAGQCRSVRDEEKPGCVVSQVQAESLSRPVEDSTSSGQISPAEESSASLDDASAGSQLEDDLGVKVESCVDRAERAARYTKTKEEMDAAKAAALQARQAELDARKESSLRERSAIARIQFRLPDGSFFTNQFPSESRLEEARQFAAQEVGNKYGNFSLATMFPRREFTGDDFDRTLLELELAPSASVVLLPTGRPANTVVQASGGGLWAVLGTLFYPLLAVWRFLSSFLLSSPPPAGPSTRQAPQPSTSAAAEPKRETVRKRVLEKRTEDFKKDGKIYRLRTQEDNEDDNNTWNGNSTQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0R2</accession>
    <name>W5M0R2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polypyrimidine tract-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221093.1">
        <property type="nucleotide sequence ID" value="XM_015365607.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0R2"/>
    <dbReference type="FunCoup" id="W5M0R2">
        <property type="interactions" value="1873"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001974.1">
        <property type="protein sequence ID" value="ENSLOCP00000001969.1"/>
        <property type="gene ID" value="ENSLOCG00000001702.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688819"/>
    <dbReference type="KEGG" id="loc:102688819"/>
    <dbReference type="CTD" id="321220"/>
    <dbReference type="eggNOG" id="KOG1190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244924"/>
    <dbReference type="HOGENOM" id="CLU_015171_7_1_1"/>
    <dbReference type="InParanoid" id="W5M0R2"/>
    <dbReference type="OMA" id="NMIYPVT"/>
    <dbReference type="OrthoDB" id="296632at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001702">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903312">
        <property type="term" value="P:negative regulation of mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12777">
        <property type="entry name" value="RRM1_PTBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12693">
        <property type="entry name" value="RRM2_PTBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12695">
        <property type="entry name" value="RRM3_PTBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000036">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000162">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000018">
        <property type="entry name" value="Polypyrimidine tract-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000032">
        <property type="entry name" value="Polypyrimidine tract-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035000">
        <property type="entry name" value="PTBP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035001">
        <property type="entry name" value="PTBP1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="75"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="195"/>
            <end position="271"/>
        </location>
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        <location>
            <begin position="374"/>
            <end position="448"/>
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    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="491"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019540"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="568" mass="61469" checksum="36B9C33786AE0EEB" modified="2014-04-16" version="1">MDGRLETDLYPLGSSYATEKDSVHDITVGTKRGSDELFSSCVSNGPYIMSSSGAANGNDSKKFKGDFRSPSAPSRVIHVRKLPNDINEAEVISLGLPFGKVTNLLMLKGKNQAFLEMNTEEAAQTMVSYYSSVTPVIRNHPIFMQYSNHKELKTDNSPNQVRAQAALQAVNAVQTGSMSMSSVDTSGMGSQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADGLTAQHAKLALDGQNIYNACCTLRINFSKLTSLNVKYNNDKSRDYTRPDLPTGDSQPSIDHQAMAAAFGAPGIISASPYAGAHGFPPAFAIQQAAGLTMPGVPGALASLAIPGAAAAAAAGRLGIHGLAGGHSVLLVSNLNAERVTPQCLFILFGVYGDVMRVKILFNKKENALVQMADGTQAQLAMSHLNGQKLHGKAIRVTLSKHTTVQLPREGQEDQGLTKDFSSSPLHRFKKPGSKNYQNIFPPSATLHLSNIPPSVVEDDLKMLFASSGAVVKAFKFFQKDRKMALIQMGSVEEAIQCLIEYHNHDLGENHHLRVSFSKSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1L4</accession>
    <name>W5M1L4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Purinergic receptor P2Y13</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="EMBL" id="AHAT01022480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1L4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002277.1">
        <property type="protein sequence ID" value="ENSLOCP00000002272.1"/>
        <property type="gene ID" value="ENSLOCG00000001964.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUS2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267167"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5M1L4"/>
    <dbReference type="OMA" id="IFYLCMY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001964">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008109">
        <property type="entry name" value="P2Y13_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF10">
        <property type="entry name" value="P2Y PURINOCEPTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01735">
        <property type="entry name" value="P2Y13PRNCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="79"/>
            <end position="101"/>
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            <end position="158"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="179"/>
            <end position="201"/>
        </location>
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            <begin position="221"/>
            <end position="240"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="286" mass="33062" checksum="678CAAD57980514B" modified="2014-04-16" version="1">YLKNLIIADLLMTVTILLKTLSEASFAPRQLKIITCRYSSVVYYFTLYISIILMTLISLDRYLKIVRPFGTSFVRNKTFGKTAAVIIWVALFSTTAMPNMILTDPRRDNASGTLNCICLKEALGQDWHYAVIKINMVIFWVCFAVMVVFYLLISKEIYESYRRSQSNNKKDKRRAKTRVYVIIVVFFLCFAPYHFARIPYILKTPGRSCSLSKSLHLVKEFTLWLTSTNTCLDPLIYLYLCKSFRRRFIRTLQLGCEYCNASEITSKRPIKLKNTETRAGTSLCPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2A5</accession>
    <name>W5M2A5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC107079557</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220166.1">
        <property type="nucleotide sequence ID" value="XM_015364680.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2A5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002519.1">
        <property type="protein sequence ID" value="ENSLOCP00000002513.1"/>
        <property type="gene ID" value="ENSLOCG00000002157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079557"/>
    <dbReference type="eggNOG" id="ENOG502SBCW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204979"/>
    <dbReference type="HOGENOM" id="CLU_1348552_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2A5"/>
    <dbReference type="OMA" id="YTSVTWY"/>
    <dbReference type="OrthoDB" id="9422899at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002157">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15193:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15193">
        <property type="entry name" value="CD83 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865533" evidence="2">
        <location>
            <begin position="23"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="18"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="203" mass="22535" checksum="65A11F8B861139CE" modified="2014-04-16" version="1">MLVYWAAILCVAVVLQRPFSKAQDLVVADCNTEATLPCTAVQRATQYRSVHWYKVDGNRTGILRKTNNKVSLYASAKEAKLGPGEALVLPSVKPEESGIYQCSLYAVLGHRNQDSLITLKVEECVTSIMSTTVPVQQRITTVLTGEENSTELCDQVILLPSFVVVSGFTAVGLLKTALSFACVAVFLKICEKREKRMERQYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2Y9</accession>
    <name>W5M2Y9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2Y9"/>
    <dbReference type="FunCoup" id="W5M2Y9">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002747"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002753.1">
        <property type="protein sequence ID" value="ENSLOCP00000002747.1"/>
        <property type="gene ID" value="ENSLOCG00000002334.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162649"/>
    <dbReference type="HOGENOM" id="CLU_001570_22_3_1"/>
    <dbReference type="InParanoid" id="W5M2Y9"/>
    <dbReference type="OMA" id="QCINHEN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002334">
        <property type="expression patterns" value="Expressed in zone of skin"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046222">
        <property type="term" value="P:aflatoxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000010">
        <property type="entry name" value="cytochrome P450 2W1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF319">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AC, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="501" mass="56105" checksum="68E27DFDA49ADB25" modified="2014-04-16" version="1">AKMFLPEGFLQLSTVTLLLAAVIVISVVYIFMGSTPESAKTPPGPPALPLLGNLLSLDLKRLDLSLVELSKTYGSVFTVHLGPMRVVVLAGYKTVKEALVDYPEEFGSRLITPIFHDINQGHGIIFANGNSWKEMRRFALSNLRDFGMGRKGSEEKIIEEIQYLTEVFEAHGGKPFDTTEPVNCAVSNIISSIVYGDRFDYADPVFQAMVKRANHMIKLSGSPAIQLYNIFPFLGFLLKARKKILMEVAENIADMKNIVKQLQQTLQEHDKRGFVDAFLIKQQELSGQRDSHFHDDNLIHASGNMFGAGTETTGTTLRWGLLLMAKYPHIQKKVQEEIEKVVGTKQPRAEHRRSMPYTDAVIHEIQRLANVVPLNLPHMTAADVHFQGYFIKKGTPVFPLLTSVLQDETQWETPHQFNPAHFLDPEGQFVKKDAFMAFSAGRRACLGESLARMLFLIFTSLLQKFTFSPPPGVQESELDLTPVLGLTMNPSLHQLCALRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3G2</accession>
    <name>W5M3G2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01032892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3G2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002926.1">
        <property type="protein sequence ID" value="ENSLOCP00000002920.1"/>
        <property type="gene ID" value="ENSLOCG00000002490.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502TDR8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590207_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3G2"/>
    <dbReference type="OMA" id="HEYHAPG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002490">
        <property type="expression patterns" value="Expressed in zone of skin and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="15593" checksum="3750894D92E987D4" modified="2014-04-16" version="1">SLSTASTVSTTSPASIVSTTSMASIVSTASTTSIASAASTGSAVSAASTTSPASMVSTVSTISIASTGSTTSTASTTATASTASTASTTSTASTRSTASAASTTSTVSTTSIASMASTASRTSRASTKSIASMASTASTTFIASTTSIASTASTTSTASTASTVTTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3K7</accession>
    <name>W5M3K7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Shox homeobox</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638959.1">
        <property type="nucleotide sequence ID" value="XM_006638896.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3K7"/>
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        <property type="gene ID" value="ENSLOCG00000002524.1"/>
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    <dbReference type="GeneID" id="102694897"/>
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    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000154287"/>
    <dbReference type="HOGENOM" id="CLU_047013_5_0_1"/>
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        <property type="component" value="Linkage group LG17"/>
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    <dbReference type="Bgee" id="ENSLOCG00000002524">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="term" value="P:bone mineralization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:pectoral fin development"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="Short stature homeobox 2"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
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    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
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        <property type="entry name" value="Paired_homeobox_Bicoid"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46255:SF2">
        <property type="entry name" value="SHORT STATURE HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="107"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="10">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="109"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="284" mass="32084" checksum="0A554167D5F334B4" modified="2014-04-16" version="1">MEELTAFVSKSFDQKAKESNKKESITYREVLESGLARARELGNSETNLQDITETSNHCPVHLYKDHVELEKEKLKEFNVTRASEGIYECKEKKEEVKSEDEDGQSKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQMHKGVILGTASHLDACRVAPYVNMGALRMPFQQVQAQLQLEGVTHTHPHLHPHLAAHAPYLMFPPPPFGLPIASLADSASAAAAVAAAAKSNSKNSSIADLRLKARKHAEALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M539</accession>
    <name>W5M539_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640898.1">
        <property type="nucleotide sequence ID" value="XM_006640835.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M539"/>
    <dbReference type="FunCoup" id="W5M539">
        <property type="interactions" value="1043"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003497"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003504.1">
        <property type="protein sequence ID" value="ENSLOCP00000003497.1"/>
        <property type="gene ID" value="ENSLOCG00000002970.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693255"/>
    <dbReference type="KEGG" id="loc:102693255"/>
    <dbReference type="CTD" id="6827"/>
    <dbReference type="eggNOG" id="KOG3490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018559"/>
    <dbReference type="HOGENOM" id="CLU_138052_3_0_1"/>
    <dbReference type="InParanoid" id="W5M539"/>
    <dbReference type="OMA" id="FDGMIAV"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002970">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000001">
        <property type="entry name" value="Transcription elongation factor SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="13">
        <location>
            <begin position="13"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="13202" checksum="1FE0FE5D95B9B874" modified="2014-04-16" version="1">MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCEAYLQMKGNREMVYDCTSSSFDGVIAMMSPEDSWVAKWQRISNFKPGVYAVTVTGRLPPGIVRELKSRGVIYKSRDTAVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M892</accession>
    <name>W5M892_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Potassium channel subfamily K member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M892"/>
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        <property type="component" value="Linkage group LG18"/>
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            <begin position="168"/>
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            <begin position="38"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
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        <source>
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        <source>
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    <sequence length="377" mass="42646" checksum="8FC844D58823434C" modified="2014-04-16" version="1">MKKQNVRTLSLILCMFSYLLVGAAVFDALESESESSRKRILEQKRNEMKKKYRFSEDDYREIERVVLQAEPHRAGRQWKFAGSFYFAITVITTIGYGHAAPGTDAGKVFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLLKKIKECLGMRNTDVSMENMVLVGFLSCIGTLCIGAAAFSHFEGWTFFHAYYYCFITLTTIGFGDFVALQKKEDLQEKTPYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASVKRERDMIALSLGDSGRSRSTLFLPMQRPSKAGSRLGSFCSCICYRSQNCDSPATSQHENLGCHTNSVYYNSISYRIDGGLLSTRDNTALSSPGSTFTSHPSFTGYLRTRRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8F5</accession>
    <name>W5M8F5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor 3B subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pre-mRNA-splicing factor SF3b 155 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Spliceosome-associated protein 155</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="1870"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004663"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004671.1">
        <property type="protein sequence ID" value="ENSLOCP00000004663.1"/>
        <property type="gene ID" value="ENSLOCG00000003899.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="LVMNYVW"/>
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        <property type="component" value="Linkage group LG12"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
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    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
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    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
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        <property type="term" value="F:mRNA binding"/>
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        <property type="term" value="P:definitive hemopoiesis"/>
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        <property type="term" value="P:hematopoietic stem cell differentiation"/>
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        <property type="term" value="P:melanocyte differentiation"/>
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        <property type="term" value="P:myeloid cell development"/>
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        <property type="term" value="P:regulation of apoptotic process"/>
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        <property type="term" value="P:spliceosomal complex assembly"/>
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        <property type="entry name" value="Splicing factor 3b, subunit 1"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015016">
        <property type="entry name" value="SF3b_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08920">
        <property type="entry name" value="SF3b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Splicing factor 3B subunit 1" evidence="13">
        <location>
            <begin position="342"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="14">
        <location>
            <begin position="1099"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="176"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="860"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08920"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22646"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004663.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1317" mass="147159" checksum="90E59D9ED8F95C1F" modified="2014-04-16" version="1">MAKIAKTHEDIEAQILEIQGMKAALLEEGERGVGLDSTGYYDQEIYGGSDSRFTGYVTSIAANEHEDDDEEDSSTSLLGQKKPGYHAPVALLNDIPQSAEQYDPFAEHRPQKIAEREDEYKARRRQMIISPERLDPFADATGGKTPDPKLQARTYMDVMREQHLTKEEKEIRQQLAEKAKSGDLKAVNGSAASQAAAKRKRRWDQTADQTPGSASTTPKKASSWDQADTASETPGHTPSQSRWDETPGRARGSETPGATPSTRMWEPTPSHTPAGAATPGRGDTPGHATPGHGGASSSVRKNRWDETPKTERGAETPGHGSGWAETPRTDRGGDSVGETPTPGASKRKSRWDETPASQMGSSTPLLTPGKTPIGTPAMNMATPTPGHLMSMTPEQLQAWRWEREIDERNRPLTDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPIGGMTGFHMQTEDRSMKAVNDQPSGNLPFLKPDDIQYFDKLLVEVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDEKNTYIRFELEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8K3</accession>
    <name>W5M8K3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Kirre like nephrin family adhesion molecule 3, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8K3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004711"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004719.1">
        <property type="protein sequence ID" value="ENSLOCP00000004711.1"/>
        <property type="gene ID" value="ENSLOCG00000003936.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160603"/>
    <dbReference type="HOGENOM" id="CLU_013520_1_2_1"/>
    <dbReference type="InParanoid" id="W5M8K3"/>
    <dbReference type="OMA" id="TNFTCQA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003936">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032836">
        <property type="term" value="P:glomerular basement membrane development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039022">
        <property type="term" value="P:pronephric duct development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039021">
        <property type="term" value="P:pronephric glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05759">
        <property type="entry name" value="IgI_2_KIRREL3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000232">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF51">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="3"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="111"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="222"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="307"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="391"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="543"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="622"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="738" mass="79194" checksum="4B7B087C84471F1A" modified="2014-04-16" version="1">HLPHILSLSSSSSVPAGRAAYFSQEPKDQVVVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWERYSIMGDASTGEHSLFIDSAELGDDAVYECQATQAALRSHRAKLTVLVPPGDPVVEGGPVVKLKAHAPHNLTCRAAGAKPAAEIAWYRDGELQESAVYSKSVMEDGKRETAVSMLLVTPEDKDTGRTFTCRVTNPAAPAGKQISVALNVQHPPSVTLSVQPQTVMEGSKVLFLCSASANPEITGYRWAKGGVPISEANGDSLEVTVDHTYFTDPVSCEVSNSVGSTNVSTLVDVQFGPRLLSEPRPLVVDIGMDAAFTCSWTGNPPLTLAWTKVGSSVVLSNGNTLQLKAVTQEDAGTYSCKAIVPRIGVAEKEVTLTVNGPPIITADATQHAAKNTKGKLECLVGSSPPPDKIVWSFGETTLASGTSGRFSVTTVTTDRGTLSSLVIAETLPEDFALRYNCTAWNRFGTGTVLVTLKEQEALPLVIIVGAAVGGGCVLLIFLITVVSLCCRNSGKGKKSARLSKSDIRVQIVHSDHNAARANEDEEDVKEPMAPNSESPGTSRTEHSDLLEEEEDERSDIKDPTNGYYNVRTHEDRHLRSGFSEYVPAARPVYTPSQLPSPSPLYGQPRVYDYSSRYGTASGGRPSYEPPPPPPERAYAPEALYPGSAYLPATYGRAFTSYVKPSAYEKVEAYEHSDQASKASSSSRFSYASSHEYGRPLHQRMQTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8Z6</accession>
    <name>W5M8Z6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prothymosin alpha-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pro/parathymosin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642006.1">
        <property type="nucleotide sequence ID" value="XM_006641943.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8Z6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004863.1">
        <property type="protein sequence ID" value="ENSLOCP00000004855.1"/>
        <property type="gene ID" value="ENSLOCG00000004066.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682611"/>
    <dbReference type="KEGG" id="loc:102682611"/>
    <dbReference type="eggNOG" id="ENOG502S79P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287715"/>
    <dbReference type="HOGENOM" id="CLU_166236_0_0_1"/>
    <dbReference type="InParanoid" id="W5M8Z6"/>
    <dbReference type="OMA" id="HETIFMT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004066">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Pro/parathymosin"/>
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        <property type="entry name" value="PROTHYMOSIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTHYMOSIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prothymosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="120" mass="12935" checksum="A097B7F9AF65CD56" modified="2014-04-16" version="1">MADTAVDTTTTTEVTPKDLKEKKEVAEEVEKTEKENGSGDAPANGTEANGADHGEETPEDEEEEDEEAEGEGEEDEEGEEGAEEEADGHPVKRPAEEEEKVETKKQKTENGDSTEAEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9F6</accession>
    <name>W5M9F6_LEPOC</name>
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        <submittedName>
            <fullName evidence="9">Src like adaptor 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006639571.3"/>
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        <property type="protein sequence ID" value="ENSLOCP00000005015.1"/>
        <property type="gene ID" value="ENSLOCG00000004198.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502QPJN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Linkage group LG18"/>
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    </dbReference>
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        <property type="term" value="C:COP9 signalosome"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
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        <location>
            <begin position="32"/>
            <end position="92"/>
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        <location>
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            <begin position="1"/>
            <end position="30"/>
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            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005015.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="267" mass="30217" checksum="5D6B225BE9B10EE7" modified="2014-04-16" version="1">MGSRPSKGRRNSSQQANLSNQQEPPQAAARDPNKYVVVALYNYPSGGPGDCSIRVGERLNILSEEGDWWRVSSTATGKESYIPSNYTAKVFHRWLFEGISREKAEELLLLPYNQAGSFMIRESQSQRGAFALSVRRSTTSSWDSVKHYRIRRLENGWFYISPRLTFASLIQLVDHYSESGEGLCCMLREPCYIQGSNNVPVLTGPPPTAVRKPTLNWKDVDRSMLFSNDKDTTEESLVSEGLREAINSYLYMTEEMGCVDCIDSWKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9J1</accession>
    <name>W5M9J1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">ADAM metallopeptidase with thrombospondin type 1 motif 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000004181.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682273"/>
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        <property type="component" value="Linkage group LG26"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057401">
        <property type="entry name" value="Adt-1/2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013277">
        <property type="entry name" value="Pept_M12B_ADAM-TS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF41">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25379">
        <property type="entry name" value="Adt-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01861">
        <property type="entry name" value="ADAMTS8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865893" evidence="19">
        <location>
            <begin position="21"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="20">
        <location>
            <begin position="225"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="300"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="329"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="346"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="475"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="540"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="544"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="946" mass="104421" checksum="2D58846F1273EFCD" modified="2014-04-16" version="1">MRLWVHLALLIFAVTNSALSAELFESEEVVPVRISGRNSGRFWKRSEDLPSYKLVAFGKDFVLNLTPDDSFLAPTIKILRIKAKYLHVLSDLDAPSAEAEKLRNINETEDGEDLRRCFYSGTVGFNQDSVVAVSLCNGIRGSFITEGDEYLIEPKVSGKGASEKSATQLHVIKRRVLASTKGESKVTFDQMEDEKQENPGGSILFKRAAEARRMARKKRFVSAARYVETLLVADPSMAQFYGEEIKHYILTLISVAAQIYKHPSIKNSVNMVVVKVLIVEDEEVGPSVSSNGGLTLRNFCSWQRHFNPSSQRHPEHYDTALLFTRQDICGHQSCDTLGVADVGTMCDPTRSCSVIEDNGLQAAFTAAHELGHVLSMPHDDSRNCERLFGDLGEHHIMAPVFIHLNKTLPWSPCSALYITEFFDNGHGDCLLDAPEKAIPLPTGLPGLAYGLDRQCQQIFGEEFMHCPNTSEADVCSQLWCRVEGQPHCTTKNGSLPWADGTACGANRSCLDGECRETEEILKPKAAVDGNWGAWGPWGQCSRTCGGGVEFSYRECTDPEPQHGGKYCEGQRVKYQSCNIQPCEDNNGKSFREEQCEKYNSFNYVDIHGNVKQWIPKYAGVSPRDRCKLFCRARGSSEFKVFEAKVIDGTPCGPDTTSICVQGQCIKAGCDQVIGSNKKLDKCGMCGGNGSSCRKITGSLNKAVYGYNDIVTIPAGATNIDVKQRSHRGIKHDGNYLAVRAEEGEYILNGNFSVSTVEQDIPVSGAVLRYSGSSTTLERLQSFHRLREPITVQLLATAGEVSPPKVKYTFFIPKDVSFTKVKGKKASPHVIQPIGMSQWSLGEWSECSKTCGSGWSRRNVDCRDGHGQLSGLCDGDLKPADIRPCGDIPCPIWQMGPWSSCSRTCGQGERRRSIFCIDYAGKTVEPENCDLAKQPNPVSGECFYQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9R6</accession>
    <name>W5M9R6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 222 member Aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9R6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005125"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005133.1">
        <property type="protein sequence ID" value="ENSLOCP00000005125.1"/>
        <property type="gene ID" value="ENSLOCG00000004291.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPV4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063811"/>
    <dbReference type="InParanoid" id="W5M9R6"/>
    <dbReference type="OMA" id="QQHLRMY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004291">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029340">
        <property type="entry name" value="FAM222"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16070:SF2">
        <property type="entry name" value="PROTEIN FAM222A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16070">
        <property type="entry name" value="PROTEIN FAM222A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15258">
        <property type="entry name" value="FAM222A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="432" mass="44741" checksum="773CBD0F0655AB05" modified="2014-04-16" version="1">LAAPMHSPRYPSPAELDAYAQKTANSPLSIKIFPTNIRVPQHKHLNRTVNGYDTTGQRYSPYPHLHTGGYQGLLAIVRAASAPTVKGVVKNSEGKRTKLSPAQIAVAPYPPPSSSTLAHGHGQIPYHTGPPKPPEAPTLSVPPNVTVAASVIPIAGGRGLALPPQSNLPSIQSIIYQINQHCQAQALQQVCPGVVVAAAAAAAATSANPSPAKQSAAAAASSNSSSNGFATGMAPQGSLAYTGAMLPAQGAEIAKAGTAYMDSMDYLLSPETCVPGGAGGVVAGGAQVSSRPYALTASAGGLDKVSSSPLNCAGMHGNFSVGQYFAPPWNSVLVTPDSDCYNPQDLAGVNTGGAGAGHRELGFPHHHHHHLPSKSLCNTSILSSSLQSLEYLINDIHPPCIKEQMLGKGYETVSVPRLLDHQHAHIRLPVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBV3</accession>
    <name>W5MBV3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01025176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBV3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005862"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="73"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="231" mass="24990" checksum="2C51502C8BB8D5BA" modified="2014-04-16" version="1">QILLCSTSSRTTRTRPASSMDFRNTRNVAVAATMSVLMSRLIVHYNSSIKLGALAFAATRMYTAQPKKNPVVYFDIAADNEPLGRVTFELNADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPQFMCQGGDFTNHNGTGGKSIYGPRFPDENFILQHTGPVGVLSMANAGPNTNGSQFFICTAKTDWLNGKHVVFGHVAEGMDVVSKMEKLGSRGGSPSKRIVITACGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCB8</accession>
    <name>W5MCB8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">von Willebrand factor C domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022006">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015357171.2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006027"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006035.1">
        <property type="protein sequence ID" value="ENSLOCP00000006027.1"/>
        <property type="gene ID" value="ENSLOCG00000005007.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688184"/>
    <dbReference type="KEGG" id="loc:102688184"/>
    <dbReference type="CTD" id="375567"/>
    <dbReference type="eggNOG" id="ENOG502QW4Q">
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_073865_0_0_1"/>
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    <dbReference type="OrthoDB" id="8574072at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    </dbReference>
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        <property type="entry name" value="BRORIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866026" evidence="8">
        <location>
            <begin position="28"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="208"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="35251" checksum="C8F4B6D4EED9BF68" modified="2014-04-16" version="1">MRHSLAMTIGILSRALLFMCCLMGVLCNPTIPLQMSQERLERALTQSKEDQASKSGLSQATQERLEEISRMGRDEVNKQGKTKGNKTSFSKGRMGWSSQDTGSKSEKQQEQQEVSLEQMDEGPTSESNVTPDSLDEYAYPDYRGKGCVDESGFVFAIGEKFTPGPSTCPCLCTEEGPLCAKPECPKVHPRCIRVDTSQCCPQCKERKNYCEFRGKTYKSLEEFKVSACEKCRCESSGEVLCTVSACPQTECVDPEYEPDQCCPICKSGPNCFADNAVIPAGREVKIDDCTICYCTYEEGTWRIERQATCSKNECKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEC3</accession>
    <name>W5MEC3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637721.1">
        <property type="nucleotide sequence ID" value="XM_006637658.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216809.1">
        <property type="nucleotide sequence ID" value="XM_015361323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEC3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006732"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006740.1">
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        <property type="gene ID" value="ENSLOCG00000005574.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683467"/>
    <dbReference type="KEGG" id="loc:102683467"/>
    <dbReference type="CTD" id="325034"/>
    <dbReference type="eggNOG" id="KOG0578">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182988"/>
    <dbReference type="HOGENOM" id="CLU_000288_26_6_1"/>
    <dbReference type="InParanoid" id="W5MEC3"/>
    <dbReference type="OMA" id="DYCNANC"/>
    <dbReference type="OrthoDB" id="2914378at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="PAK3-like"/>
    </dbReference>
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        <property type="entry name" value="PAK_BD"/>
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    </dbReference>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBD"/>
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        <property type="entry name" value="Pkinase"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="CRIB"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="254"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="529" mass="58599" checksum="91CCDC30877F38F4" modified="2014-04-16" version="1">MSDNGELEDKPPAPPVRMSSTIFSTGGKDTLSANHSSKPLPSVPEERKPRNKIISIFSADKGGRKKDKDKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNTRQKYLSFSSSEKDSFPSGTQTPAKKGMEPSPATTLKEIDDDEDEETPPPIVAPRPEHTKSVYTRSVIDPIPPPPVNPDGDSASKAADRQKKKAKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEL8</accession>
    <name>W5MEL8_LEPOC</name>
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        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219931.1">
        <property type="nucleotide sequence ID" value="XM_015364445.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEL8"/>
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        <property type="interactions" value="602"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006827"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006835.1">
        <property type="protein sequence ID" value="ENSLOCP00000006827.1"/>
        <property type="gene ID" value="ENSLOCG00000005654.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688020"/>
    <dbReference type="CTD" id="170591"/>
    <dbReference type="eggNOG" id="ENOG502S17E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161125"/>
    <dbReference type="HOGENOM" id="CLU_138624_2_1_1"/>
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    <dbReference type="OMA" id="QYSCKEG"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005654">
        <property type="expression patterns" value="Expressed in larva and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
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        <property type="entry name" value="EFh"/>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
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    </dbReference>
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        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="99" mass="11333" checksum="240973FAD317AB5A" modified="2014-04-16" version="1">MPTQLERAMDALINVFHNYSGNEGDKYKLNKGELKQLLNSELTDFLTSQKDPMLVDKIMNDLDSNKDNEVDFNEFVVLVAALTVACNDFFEEQQKKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MET8</accession>
    <name>W5MET8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MORN repeat containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MET8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006905.1">
        <property type="protein sequence ID" value="ENSLOCP00000006897.1"/>
        <property type="gene ID" value="ENSLOCG00000005712.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161806"/>
    <dbReference type="HOGENOM" id="CLU_032017_4_1_1"/>
    <dbReference type="InParanoid" id="W5MET8"/>
    <dbReference type="OMA" id="WNEDEPN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005712">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF263">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="248" mass="28036" checksum="A02D6FA2E004D3F0" modified="2014-04-16" version="1">MAASQKQQRNSQYYVGEVKSLLRDGYGLYVYPNSFFRYEGQWKLGKKHGHGKLVMKDGSYYEGEFVNGEIEGNGLRYWACSVGNTYSGQFNSGELHGYGVMQYGTGERFEGEFSFGLREGHGFLVDKEGQIYEGSFHKNKKHGEGQMTYRNGDQYEGDWILDQRQGHGVMHFADGSIYEGQWRNNLFNGQGTMIHCSGVIYEGLWTSGQPAGGASKIVIEGGDMLEVFQRSPFTLEVQLQNEEEEIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG99</accession>
    <name>W5MG99_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG99"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007408"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007416.1">
        <property type="protein sequence ID" value="ENSLOCP00000007408.1"/>
        <property type="gene ID" value="ENSLOCG00000006131.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166211"/>
    <dbReference type="HOGENOM" id="CLU_010691_0_0_1"/>
    <dbReference type="InParanoid" id="W5MG99"/>
    <dbReference type="OMA" id="PFAFPRH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006131">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045345">
        <property type="term" value="P:positive regulation of MHC class I biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045348">
        <property type="term" value="P:positive regulation of MHC class II biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001028">
        <property type="entry name" value="Class II major histocompatibility complex transactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008095">
        <property type="entry name" value="MHC_II_transact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189:SF1">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01719">
        <property type="entry name" value="MHCIIACTVATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="205"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="942" mass="105433" checksum="07EC3545E0218E69" modified="2014-04-16" version="1">MNNAAVQVYPSFPQHIIATPHTSPVGSSVNSRGPTFVLVPPVSLTSSQPQASPSSPAPGSVVGPESVNQTSVISSADSISTLSPASVSSPASTADTGMSPHKGSTAPPLQSSQCSEMPKCVEDYIATAKAFMYDNCQLMAIENEVALDSLYIDVPLVQRQIKTRTGKHVNKYQEKELLLFDMAERRKFEIKRSQLFEEAGQKPKELIALLGKAGMGKSTFIQKLCLDWSKGDFQHFELVFFFETKKLNLPDQTYSLKNLLFDIFPAPPPRNVDEVFRYILSNPQKVLIIFDGFDDFQDSEGLLQCPATSSTKDVYTIKQLFSGLFQKKLIVGSTILIAARPKEVLNQFLGKVDKIVELCGFSEEEIQLYTERYFKDTSCYDWAWTKVRNHNFLSALCFNPLICRFTCFLLENVGSKKSLPSTLTELYQQVFYQKLHMNTTKADPGKRQEKQNIPKLGALAWRGVKDHSCLLSHEDLCSTGLKEFGLNSGILTPYILKRKGDEEEFGCGFTDLFVQNFLGALHITAAKELNDKSLLTQTMLQQKKKKPQDDWLDVTRRFMVGLLFQKTQKHLDWLPGSTKKRLAKRNTIEAHLEKLRPSELSANRLLELCHCVYETGSTKLLRNLAQKLPEELSFSGTPLTPPDVFVLKYLLKETKDNQKNFSIDIQETAIDLPGLRELVGLNCVTSFRASVADTISLWECLQQTGEKDLLKDAVAKFTVNPFKARQLSDVENLNQLVQIYRQKKLLLCDSADSETRPDGDTFHVPAVNNLRKLEFALGPENGYLGFLRLVEVLPTLQWLQHLDLEGLTCNKIGDRGAEKLAEILPCLSSLETLNLSQNCIGDQGAEKLAQTLPSLLSLRSLSLYSNSIADSGAESMAQILPEMTSLTDLDVKFNKITDVGALKLSESLRRCPWIKSIAMWNHSIPHGVLEHLKQKDSRINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGC1</accession>
    <name>W5MGC1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formin binding protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGC1"/>
    <dbReference type="FunCoup" id="W5MGC1">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007430"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007438.1">
        <property type="protein sequence ID" value="ENSLOCP00000007430.1"/>
        <property type="gene ID" value="ENSLOCG00000006124.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTDD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003450"/>
    <dbReference type="InParanoid" id="W5MGC1"/>
    <dbReference type="OMA" id="QDGDGHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006124">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Formin binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053076">
        <property type="entry name" value="WW_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697:SF1">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="920"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="938"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1026" mass="110543" checksum="DDA16F0ECFEA1893" modified="2014-04-16" version="1">MGKKTRTGTGRRTILQLSPPGPRGFPAGGAGCREEGAGSGSEGSSVTEPLNIIELRVTRAVMTALMREEYEQDGSGDASHPQISKDNLMETKPPSVKATGGLSLLGAYEDSEEEDGADSQPAPSNANHNQSADIDSTLANFLAEIDAITTQPGQAAERTTVASSAPAPTPPRPDPKDQPQTPRHPAGQTGCEEAGTGAEFQYDTQYSLVGAVGVEMGDWQEVWDENTGCYYYWNTHTNEVTWQLPHYLAHQVQDLQQYPDSSSVAVNGTSTTQSGGFYSEQNSTVPAAVPAVKKEVKKEVMESVVALTSEEEERQGVAASLLAPLIPDEVKEAEERWRKKVISVVEGKQESGEGEGQPVEDPAPAEADAHSQGDPRSNAQSGESSEGEAVEEEEEMEEDTIELELALERKKAELRALEEGDGSAGGSSPCSDVSQEGGRSALLRKPKWKTSFLRSASPESNSRGSEKAGRNSPDPPDTAHSKLNEKQEEDEQVEAEVSVEKPKPRPVPKEEEETADLKFQIGELANTLTSKMEFLGINKKTISNFQLLLLQTETRITDWREGALNGNYLKRRLQEAAEQIKHYELNATPKGWLCHWDREHRRYFYVNDQTSASQWEFPDVDGEKEAEGGGTSGPAEHSSVVPGDLKPPPEPPGVYTGQRSHAQSCRAVFSLTTCASSFMGLAPVQQGMASYWTLTQPPLPPPTDPPLPDECPPPPPPPPDSPPPPPPPPLSEDGEIEEVEMEDEDGEPPAPGTEEEASRKPAQPTAGSTAKPCEPARPPSPPAKAVKRRAPSSAPGPAQKTVTIGSSPILYSQPAVSAGESTTAFWEQGAEPNSKPCLGNFIKAGVNHSVKEGTCLSISVAISMRIISYICLCTSVPAGPMMSAPVYWGVSAVAVPTPPAETGAPTGPAPPSQPPPPAPSSSVADQAAGKMQAMEKMKKVKKDKAKKSKTKMPSLVKKWQSIQKELDEEEKSSSSDEDRDQQNHKRIEEWKQQQLVTGKAGKNANFEALPVDWRDRLKRRKVMQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGS0</accession>
    <name>W5MGS0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01013597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGS0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007579"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007587.1">
        <property type="protein sequence ID" value="ENSLOCP00000007579.1"/>
        <property type="gene ID" value="ENSLOCG00000006270.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166046"/>
    <dbReference type="HOGENOM" id="CLU_025879_1_1_1"/>
    <dbReference type="InParanoid" id="W5MGS0"/>
    <dbReference type="OMA" id="YKMTPQT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006270">
        <property type="expression patterns" value="Expressed in intestine and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000167">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000191">
        <property type="entry name" value="Protein disulfide isomerase like, testis expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000456">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000042">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF58">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE-LIKE PROTEIN OF THE TESTIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868169" description="protein disulfide-isomerase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="13">
        <location>
            <begin position="373"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="506"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="530" mass="60031" checksum="886225F4327A1DF7" modified="2014-04-16" version="1">ASMKTPSLFLLSAACLCAAVAGNSTLESSGDSVPPAKKEKPAKINEENGVLVLKKSNFDQALRQHKYLLVDFHAPLSGDSQSLSREFTAAAARLKSQGSRIRLGLIDVSKEKDLANELNVTAYPALWFYISGDKENPVICPAVLRTATSIVTWLERRTGRSAELIKDRSQAEEFFKLGDVVVLGFFQDLEEGAVKTFYEAAVDIPDLPFGVTKNKSLFSKYKVRKDRDTVVLFKQSEVLKEYEVSSETLKESLIKFLRVHEMDLVTEYNGETSVKIFGSLVTNHILLFANKSAEDFRDLHGTFEAVAAEFRGEVLFIFVDTDESRNGRIFEYFRVRDVDAPAVRIVNLTDNVQYQMQAEEVTVDNVRKFCGDFIDGKAKPKLESEPIPENWDKHPVKELVAVNFDRVVFNDERNVFVMFYAPWSKECQELFPVWEKLGKVYGHHETVVIARIDSAANDVHSVLQERHPAFKFFPAVLAEKVVPYAGKRTLEDFVQFVEDQIELAKEQRAKEEEDTRKMAEPENSEGNQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGY1</accession>
    <name>W5MGY1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox C11a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGY1"/>
    <dbReference type="FunCoup" id="W5MGY1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007640"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007649.1">
        <property type="protein sequence ID" value="ENSLOCP00000007640.1"/>
        <property type="gene ID" value="ENSLOCG00000006316.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160009"/>
    <dbReference type="HOGENOM" id="CLU_472474_0_0_1"/>
    <dbReference type="InParanoid" id="W5MGY1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006316">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046333">
        <property type="entry name" value="HXA10/ABDB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
        <property type="entry name" value="DUF3528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="489"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="491"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="153"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="352"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="427"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="565" mass="62055" checksum="4F97D22C278C7C49" modified="2014-04-16" version="1">TSDFGDRAGCASNLYLPSCTYYVPEFSAVSSFLPQAPSRQINYPYSTNLSQVQPVREVSYSLDPPSKWHHRSNYTSCYSGDDLVHRECLPPSTVTEMLMKNESVYSHHHHHHHHPSSNHPSTGFYSGVGKNNVLPQGFDRFFESAYCGAEPPPENCVQKSEAGKLEAEHEPGALPSAADAEKDPEDEEATHISTNTRFFQPKRCQLPTAVIFLAALTPEPTMSCPNNVATGSFLMDSLVGPSAYRSEGYSAASGMYMQTGAEYGCTVMRNCGILPSSLPKRDELSPVSLPLGSYHPSPYLAQLDTWGGDPKGSCRTDQPVARPLPSCSFPAGNVKEESMCCMFRADVNGAKGPGEPTTYTRLGAERSPPQRAVPVPGYFRVSQGYPGEKAQEEGEFGPGFSSVGSLASPSETAAVAGVPVPEAFSENGEHDRDSGPAGVKSEDAAAAKRLAEPEPERLSKTDSSTDISDSEAKENSKAEKVAGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISRSINLTDRQVKIWFQNRRMKLKKLNRESRVRELSTGYSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHM8</accession>
    <name>W5MHM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abhydrolase domain containing 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007887"/>
    <dbReference type="ESTHER" id="lepoc-w5mhm8">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000007897.1">
        <property type="protein sequence ID" value="ENSLOCP00000007887.1"/>
        <property type="gene ID" value="ENSLOCG00000006528.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694905"/>
    <dbReference type="KEGG" id="loc:102694905"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="HOGENOM" id="CLU_032487_3_0_1"/>
    <dbReference type="InParanoid" id="W5MHM8"/>
    <dbReference type="OMA" id="SREYLQM"/>
    <dbReference type="OrthoDB" id="247542at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006528">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000597">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF96">
        <property type="entry name" value="PROTEIN ABHD15-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="52588" checksum="788D4AF001FC3F0D" modified="2014-04-16" version="1">MWDSVVFLLPVLVLLSSALLLKLVWNSASGVLWRLGLGLWARLRTRLGLPQPHSDRPQLICKPSALARFLLRHCGALAWPTLTPWPWGDPHVQTLHSLAWPAGGAAGRVDFAREHLELRDGGIVALDWAVAAAPGGSSSSSRSRAPPAPACPPILIVIPNSWGRRTLHLQLLCRLALAQGFYPVVFNRRGQGGCPLVTPALQPFGEPSDLMEAVSYIRCRHPSSSLLAVSEGSGSGLLLSYLGECGSSSYLTAAACLSPIFLGQQWFETPLPAPYRLGLLLYQKLQISRYGTALSAVMDVQRLLRCSSLREFEEIQFCSGGTGEKGKPPSPDWEAYWERNEPLRDVDEVAVPVLCLCSWDDPVRGDPHASLPLDLFQTSPYFLLALTGRGGHCGLRGGREGSACWSHSAVLEYFRVVGEFLQGEERGRDAGQLPRHRTSTLWTRRRRGTLLRRDRHSPAASAQLPEDLFQWQRSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI77</accession>
    <name>W5MI77_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Intersectin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Synapse</location>
            <location evidence="25">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020351">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008096.1">
        <property type="protein sequence ID" value="ENSLOCP00000008086.1"/>
        <property type="gene ID" value="ENSLOCG00000006667.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157065"/>
    <dbReference type="InParanoid" id="W5MI77"/>
    <dbReference type="OMA" id="XCSDLHL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006667">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097708">
        <property type="term" value="C:intracellular vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150007">
        <property type="term" value="P:clathrin-dependent synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08375">
        <property type="entry name" value="C2_Intersectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd13264">
        <property type="entry name" value="PH_ITSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11987">
        <property type="entry name" value="SH3_Intersectin1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11989">
        <property type="entry name" value="SH3_Intersectin1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11991">
        <property type="entry name" value="SH3_Intersectin1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11993">
        <property type="entry name" value="SH3_Intersectin1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Intersectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000069">
        <property type="entry name" value="Intersectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000024">
        <property type="entry name" value="Intersectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000041">
        <property type="entry name" value="Intersectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000029">
        <property type="entry name" value="Intersectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000055">
        <property type="entry name" value="Intersectin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000046">
        <property type="entry name" value="intersectin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000122">
        <property type="entry name" value="intersectin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032140">
        <property type="entry name" value="INTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF9">
        <property type="entry name" value="INTERSECTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16617">
        <property type="entry name" value="INTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16652">
        <property type="entry name" value="PH_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0168">Coated pit</keyword>
    <keyword evidence="20 28" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="16" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-0653">Protein transport</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 27" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="19" id="KW-0770">Synapse</keyword>
    <keyword evidence="13" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EH" evidence="34">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="35">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="34">
        <location>
            <begin position="217"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="35">
        <location>
            <begin position="250"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="732"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="908"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="1011"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="1083"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="1164"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="33">
        <location>
            <begin position="1246"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="31">
        <location>
            <begin position="1471"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="32">
        <location>
            <begin position="1588"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="641"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="795"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="971"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="28">
        <location>
            <begin position="350"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="28">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="825"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="846"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="971"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00074966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1730" mass="195312" checksum="9E36AF638FA08607" modified="2014-04-16" version="1">MAQFPTPFGGGLDFWAITVEERAKHDQQFHSLKPTAGFITGDQARNFFLQSGLPPPILAQIWALADLNNDGKMDLHEFSIAMKLIKLKLQGHPLPPSLPPVMKQPPCTAPTAPAFGMGGMAGMPGIPGVAPVPMAPLPVVGMSPPVVTPVPAPVPPMANGAPPMIQPLPGFAHSAATLSKGSSFSRSSTKLQKAQSFDVPNAPAASVPQDWAVPQSSRLKYRQLFNSHDKMMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGQPLPPVLPPDFIPPAFRRVRSGSGISTASLHLADQRVQEEPAEEEQQAPEKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALERQEQERRERERLEQERRRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQENIVVLKARKKTLEFELEALNDKKHQLEGKLQDIRFRLSAQRHEIESTNKTRELRIAEITHLQQQLQESQQLLGSLIPEKQSLNDQLKQVQQNSLHRDSLLTVRKALEAKESKRQQLRDQLDVVEKETRSKLLEIDVFNNQLKELREIHSRQQWQKQKEVEVEEKEQKEVELERKTAELKEDRHRRVPEEDGVSAAWSEQRDEPGGEPPPAAPAQQQWMKRVQDDDGRGRKEMQDKLNKIFMQQPEPGQLPIQAAWPPTEKSPVTGFDQDNVKVVFYRALYPFDARSHDEITIQPGDIIMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPESDVPASLKPGAGSAPKVAARVTPTSAVSAGSQPPPAETPAAPPTTTAAGSNWADFSSTWPSNNSEKTEGDGWDAWPTQPSLTVPSTGQLRQRSAFTPATITGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPMRKSMSIESGSSESPPSVKRPTPSPSKPTDPAEDQNHKCSASKFPSKEYIAMYTYESNEQGDLTFQQGDMILVTKKEGDWWTGVVGDKTGVFPSNYVKPKDSEGIGSSGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILVRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKSTPTEPPKPSGPSAVCQVIGMYDYVAQNDDELAFGKGQIINVLSKEDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEVFQKPLLESELLTEKEVGMIFVNWKELIMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPDFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPENHPDHSHLKQAQEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQTIKPLGSSGSDKVFSPKSNLQYKMYKSPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTMQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRAADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIL6</accession>
    <name>W5MIL6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hermansky-Pudlak syndrome 5 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-2 (or BLOC2) composed of HPS3, HPS5 and HPS6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HPS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030592">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030593">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIL6"/>
    <dbReference type="FunCoup" id="W5MIL6">
        <property type="interactions" value="957"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008225"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008235.1">
        <property type="protein sequence ID" value="ENSLOCP00000008225.1"/>
        <property type="gene ID" value="ENSLOCG00000006802.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155818"/>
    <dbReference type="HOGENOM" id="CLU_008734_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIL6"/>
    <dbReference type="OMA" id="EPQCMRR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006802">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031084">
        <property type="term" value="C:BLOC-2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056499">
        <property type="entry name" value="Beta-prop_HPS5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035431">
        <property type="entry name" value="HPS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056445">
        <property type="entry name" value="TPR_HPS5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287:SF18">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287">
        <property type="entry name" value="RUBY-EYE2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23756">
        <property type="entry name" value="Beta-prop_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23758">
        <property type="entry name" value="TPR_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037475">
        <property type="entry name" value="BLOC-2_complex_Hps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPS5-like beta-propeller" evidence="3">
        <location>
            <begin position="125"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="HPS5 TPR" evidence="4">
        <location>
            <begin position="696"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="628"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="920"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="923"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1013" mass="111514" checksum="E95E1B95A06E7A42" modified="2014-04-16" version="1">TVPTVPEPCSHVLAELDSLDPLLSALRLDSSRLKCTSLAASRRWLALGTSAGALHLLQREGWKQRLILTHKVRRCPPPCPAPLTEQGSLYVQTSLRQRSRSELSSVEVRRGGLVHLDSRWDVPSSREKFWRIGNKERDGEYGACFFPQTRSLGGTSPLICCARPGSRVWEASFSGEVLSTHQFKQLLACAPLPLVTHRCEPQYSPAPRSPQSIAFPRLLFLSDQYLLTWTDSAIYIFVPQSAQVLLWTEVKDLADVAVVRSELFCLHGDGRLSHLSFLPVERCVERLLRRESWVLAATVCCLFQHSIAPCRARKALPMERLEHLKAQLDGSAHRHLIGQLEEVMSTLEPVDSASSSRRSSISSHESFNVLDCGIYRVISRKGSQSDEDTSSLVSQSLSEEERLRELSSAQEEEPIEQEPQPSDGAETDRAEPALQFLPLSFRPKPPRATLQAVRDSVSSFVKKTTEKINTLQMNSDLWPRPDARGGGGRGAGPAGGVSAEPVPRRPAAPLAVGLLSSPGVACCVFCVAQGARGRPPDRAGAGAAGAEGGDGARSVSLSNPRTHAHTPCPAHARTHTPCPAHSTHTLPRAQHTHPAPRTAHTPCPARTHTPCPAHARTHTPCPARTHTPCPAHSTHTLPRARTHTPCPAHSTHTLPRAQHTHPAPRTAHTPPGPARPGPWEGERVLTRGPCLSVRAELTRSSPVVRFLEKGDLLKSLRHLRELQPWSGPLLLAHLHRLYERHGELAVRSFGQFYPTILPADVLAMSQRSHFLAYLDNLVQSQPEERRPCFLASLLQPEALRQDWLELALSHDAPQRADTLTPDGRPRWRSHLFRWGYSRLISLFIGLPADLASKQQMAETCRSHGFWLGYLSLCSELDRRAEAFSTIAWLDDMSLLEGPRGVVPESVDEWKLLLQLSQRHGDQPAAGPAPGNGVANGSADRAGRVSPENVTLRLACVVGPDRALSVLEECGARVEPGPRSGLVCELLRVAERRQRALIQAMLERCDRFLWSQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ30</accession>
    <name>W5MJ30_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008389"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008399.1">
        <property type="protein sequence ID" value="ENSLOCP00000008389.1"/>
        <property type="gene ID" value="ENSLOCG00000006938.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTX0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000221578"/>
    <dbReference type="HOGENOM" id="CLU_2078393_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJ30"/>
    <dbReference type="OMA" id="ETYINTW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006938">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13880" checksum="568E5FA7FB7430B3" modified="2014-04-16" version="1">HLDVSEEMFVWDVLAGCIDYKKLLDVVVDAFYARCGKSFLHSERNVFAVICYLATFLLEELGLQHFSNIVKSQDVNKMYKFLGFFFDVTNLSTWIKDEWSHIYDAPFVENNWITPLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJG7</accession>
    <name>W5MJG7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-ureidopropionase</fullName>
            <ecNumber evidence="9">3.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-alanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-carbamoyl-beta-alanine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes a late step in pyrimidine degradation. Converts N-carbamoyl-beta-alanine (3-ureidopropanoate) into beta-alanine, ammonia and carbon dioxide. Likewise, converts N-carbamoyl-beta-aminoisobutyrate (3-ureidoisobutyrate) into beta-aminoisobutyrate, ammonia and carbon dioxide.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, homotetramer, homooctamer; can also form higher homooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the carbon-nitrogen hydrolase superfamily. BUP family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.6" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01005183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJG7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008526"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008536.1">
        <property type="protein sequence ID" value="ENSLOCP00000008526.1"/>
        <property type="gene ID" value="ENSLOCG00000007024.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0808">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004906"/>
    <dbReference type="InParanoid" id="W5MJG7"/>
    <dbReference type="OMA" id="HWPFLRD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007024">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003837">
        <property type="term" value="F:beta-ureidopropionase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033396">
        <property type="term" value="P:beta-alanine biosynthetic process via 3-ureidopropionate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07587">
        <property type="entry name" value="ML_beta-AS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000009">
        <property type="entry name" value="Beta-ureidopropionase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="13">
        <location>
            <begin position="97"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="46424" checksum="E509FA3EC8427B3A" modified="2014-04-16" version="1">IDRDTTGKLLERSVFRALFGRDCRHMSAAQFESLESILEKHIPPEELKEVRRRLFGKELKKLEFPSDAIEAAKERDFELQGYVLEAALEQLRKPFIVRVGLVQNKIVHPTDAPVIDQITALHRRIGEIVEVASMCGVNIVCFQEAWTMPFAFCTREKAPWTEFAESAEEGHTTRFCQELAKKYNMVVVSPILERDDIHGGTLWNTAVVVSNSGNVLGKSRKNHIPRVGDFNESTYYMEGNTGHRVFQTQYGRIAVNICYGRHHPLNWFMYSMNGAEIIFNPSATVGELSEPMWPIEARNAAIANHCFTCAINRVGTEHFKNEFTSGDGKKAHSDFGYFYGSSYVAAPDGSCTPGLSRTQDGLLVAELDLNLNRQISDKWSFKMTGRYDMYAELLHKAVQPDFKPDIVKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKW4</accession>
    <name>W5MKW4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome inhibitor PI31 subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKW4"/>
    <dbReference type="FunCoup" id="W5MKW4">
        <property type="interactions" value="1022"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009023"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009034.1">
        <property type="protein sequence ID" value="ENSLOCP00000009023.1"/>
        <property type="gene ID" value="ENSLOCG00000007435.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4761">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012257"/>
    <dbReference type="HOGENOM" id="CLU_090116_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKW4"/>
    <dbReference type="OMA" id="PFGFPDI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007435">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.30:FF:000002">
        <property type="entry name" value="Proteasome inhibitor PI31 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045128">
        <property type="entry name" value="PI31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013886">
        <property type="entry name" value="PI31_Prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021625">
        <property type="entry name" value="PI31_Prot_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266">
        <property type="entry name" value="PROTEASOME INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266:SF1">
        <property type="entry name" value="PROTEASOME INHIBITOR PI31 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08577">
        <property type="entry name" value="PI31_Prot_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11566">
        <property type="entry name" value="PI31_Prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866259" description="Proteasome inhibitor PI31 subunit" evidence="13">
        <location>
            <begin position="20"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="15">
        <location>
            <begin position="45"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="14">
        <location>
            <begin position="212"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="251"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="33386" checksum="A3A104CF30628481" modified="2014-04-16" version="1">MRFHIMPTCVLWFVFHCSSRRTPQRFTGGAGPGEVRFSPGRKLRSIACPQDALVCFVHWEIVKNGYKCLGVGDQPSSSDRQSELLPAGWNSSSELYTLRYQSGDSGEQLLVKAVTVDTTLIFNVLESASGRVSDLTVSLGDYVHADSLQVFESVFKNTEELRKKVQSGLFPSASKQKTKPSEKAEKKGLRESPPCLWPDRDPLRIPQRIPRSDRQPSWQDPLAPFSAGRADLDPLGGRPGGMIVDPLRSGFPRSGFDPSAGIPGRLPPGAVPPGARFDPFGPVGANRPGPDPDHMPPPGYDDMFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML00</accession>
    <name>W5ML00_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell division cycle associated 7a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009059"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009070.1">
        <property type="protein sequence ID" value="ENSLOCP00000009059.1"/>
        <property type="gene ID" value="ENSLOCG00000007465.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155436"/>
    <dbReference type="HOGENOM" id="CLU_035988_2_0_1"/>
    <dbReference type="InParanoid" id="W5ML00"/>
    <dbReference type="OMA" id="ICVNARE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007465">
        <property type="expression patterns" value="Expressed in embryo and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902036">
        <property type="term" value="P:regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048538">
        <property type="term" value="P:thymus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF2">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="319"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="393" mass="44926" checksum="9D67CA73E04EB00A" modified="2014-04-16" version="1">PSIRMNLRSFRNTPLVPMETSSSSDDSCDSFGSDNFANTKKSLKPGSVPVKVSKILNTDSDEESFRGFEETEFSDVMGAMKVDSEEEPSAQSKRRRGSKPLKVAMKFPTRNLRKNGPAKPIPPQREDSLSDSDSDEEKGPNLMEKRAINIKENKAMLAKLMSELKTVSGIFPKRMLLPSANSPRRLSRRLVPKSFPERRSPPHTRSRSLVDGPPSPAPEDDLEDKYYLARRRRAEDDDEERAPRRRSYSGAMTIPHHVRPVEEITDSELNNICYNVREKVYNRVSVSVVLSLFFKYREXPFNQRHLALTAVQNNPLLNLCLWPYYHVGRVRKKLGQKTWFCPPCRGICNCSFCRQREGRCATGVLVYLAKYHGYDNVHAYLKSLKQELEAKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLK3</accession>
    <name>W5MLK3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cingulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cingulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLK3"/>
    <dbReference type="FunCoup" id="W5MLK3">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009273.1">
        <property type="protein sequence ID" value="ENSLOCP00000009262.1"/>
        <property type="gene ID" value="ENSLOCG00000007633.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R9EI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154489"/>
    <dbReference type="HOGENOM" id="CLU_002036_1_0_1"/>
    <dbReference type="InParanoid" id="W5MLK3"/>
    <dbReference type="OMA" id="NDETSHW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007633">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF4">
        <property type="entry name" value="CINGULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="Myosin tail" evidence="7">
        <location>
            <begin position="227"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="443"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="56341" checksum="84D7594AE8CABC48" modified="2014-04-16" version="1">MDREAAEARVRDLEDQLAGLQEELRREADSGAERDALATELVSLRAEMAEAGAARQRQEEALRQRERELTALKGALKEEVSSHDREIDQLRQQYQADIDKLRSNVEQVSMSQLSVEAERQKVNTTVKSLQKQLEESNDETSHWREQCNRLKEELRATKQELLQARLEKEEFEEELGELQERLSSMSAQLDQLQSSSPDPAQTERLTQELQLASEERDNAVLDKELLTNKLKSLEAELEAKKSSHTDRSREIRALEDKAKRLEMELEEERTSAELLTERISRSRDQIDQLRAELMQERSSKQDLELDKASMERQLKELRGRLSDLEGHQRPSANLAQLESRIQELEDRLQSEEREKSSLQSSQRRLERKVKELTIQLDEERTQFNDQKDQLALKVKALKRRVDEGEGEVERLEAARRKALRELEEQQERREQLEGKVTALEKELWRKSQSQRSVLDSTALSSDEEEGSYGPSSITSILTEGNLQTSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM19</accession>
    <name>W5MM19_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate cyclase</fullName>
            <ecNumber evidence="2">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01028299">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM19"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009428"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009439.1">
        <property type="protein sequence ID" value="ENSLOCP00000009428.1"/>
        <property type="gene ID" value="ENSLOCG00000007760.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163785"/>
    <dbReference type="HOGENOM" id="CLU_011614_4_1_1"/>
    <dbReference type="InParanoid" id="W5MM19"/>
    <dbReference type="OMA" id="KDMREPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038060">
        <property type="term" value="P:nitric oxide-cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000007">
        <property type="entry name" value="Guanylate cyclase soluble subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF10">
        <property type="entry name" value="SOLUBLE GUANYLATE CYCLASE 88E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="10">
        <location>
            <begin position="465"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="637" mass="73654" checksum="EED5F084A7128882" modified="2014-04-16" version="1">MYGLYLEAVNDYINESYGEDVWRLIEARAEIPHLKFVRHQMYNDNLILRLAKAAGEVLGKTHDELMYAFGVYMVKRIGNYGYERILKVLGRNVRDFINELDNLHEYFRFSFPKVQPPSFCVEEECETSLTLHYRSTRKGFTQFVKGQLSQVGRQFYNTDIEVEILSKEETEKMTYVVSEEQHTFVFPESFLTHTPPPTHSHLPENPKFPTAAHTDSQIYSALSALKVYKMNFDNAAFKHRMPQQKTAPGYEKLPMKRGIFFDMFPFSVIFRRDMTMYRIGDGLKEVFSDLQGKKFNEEFTLVRPMLEFSWDNIYTHLNNVFELLSKAVVESKQKDMREPPKKPLHLKGQMKYVPQWDSLIFLGTPIIETVEDMIKMGVYVNDLNLHDSSRELILAGTQQSAELQLALDQEQQKYAQLQEIIKKLDEEKKRGDSLLYAMIPKAVADRLRKGITALETCQVVFPDVTILFSDVVKFNEICIHITPMQVVDMLNEIYIVFDTLSEKHNVYKVETIRDAYMVVAGVPNKTTFHAHHICDMALDMLSSIDHLKDPSTGDNIQIRVGIHSGMVVAGVVGLKMPRYCLFGDTVNTASRMESNGVGMQIHISQTTKDHLEHEPYIIEERGKIFVKGKGYMKTYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM89</accession>
    <name>W5MM89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM89"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009498"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009509.1">
        <property type="protein sequence ID" value="ENSLOCP00000009498.1"/>
        <property type="gene ID" value="ENSLOCG00000007823.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691461"/>
    <dbReference type="CTD" id="3796"/>
    <dbReference type="eggNOG" id="KOG0246">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155570"/>
    <dbReference type="HOGENOM" id="CLU_001485_19_1_1"/>
    <dbReference type="InParanoid" id="W5MM89"/>
    <dbReference type="OMA" id="NWDTARM"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007823">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000006">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054473">
        <property type="entry name" value="KIF2A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22923">
        <property type="entry name" value="KIF2A-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 12 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="219"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="65"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="553"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="693"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="309"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="739" mass="83934" checksum="61F5FA4C51E40FDC" modified="2014-04-16" version="1">MAANFGKIVIGIYVEIKRSDGRIHQAMVTSLNEDNESVTVEWIENGDTKGKEIDLESIFALNPDIAPDEDIAPSPETPPPPTPSSVKVNKIAKNRRTLAPTKNETPSRDNRVGSARARPSQLPEQAPQPAAQQQQQQSQQQQQLQQQNARRKSNCVKEVEKLQEKRERRRLQQQELREKRAQEVDATNPNYEIMYMIRDFRASLDYRPLTTADLIEEHRICVCVRKRPLNKKEMTLKDLDVITIPSKDVVMVHEPKQKVDLTRYLENQTFRFDYAFDDTTTNEMVYRFTARPLVETIFEKGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLDLQVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCTEDVLKLIEIGNSCRTSGQTSANAHSSRSHAVFQIILRRKGKMHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKEFGISPSDIPFSQGGGSRSELSPAYEYDDFSTSPSRVKELTIDPSTVMEGRPNIHAVNQLDPLEAQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQLEQILDQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN59</accession>
    <name>W5MN59_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon stimulated exonuclease 20 like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01028255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN59"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009818"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009829.1">
        <property type="protein sequence ID" value="ENSLOCP00000009818.1"/>
        <property type="gene ID" value="ENSLOCG00000008083.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2249">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159724"/>
    <dbReference type="HOGENOM" id="CLU_022453_0_0_1"/>
    <dbReference type="InParanoid" id="W5MN59"/>
    <dbReference type="OMA" id="HAICNDF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008083">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06149">
        <property type="entry name" value="ISG20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037433">
        <property type="entry name" value="ISG20_DEDDh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF78">
        <property type="entry name" value="INTERFERON-STIMULATED 20 KDA EXONUCLEASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="205"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="12"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="386" mass="42916" checksum="03FB103DA0B3A131" modified="2014-04-16" version="1">MSDILLNLDFSCAGGFGGDKGEPHASSKHERFVRSRRYLERKGCLNKKQIPKHRPRDRDWGRDRPRDRDWGRDRDRGKDWGRPRDRDSDRGRGRDRGKDWGRPRDRKGQDQGQKQGCVQGPTPMPQHTSSHTPESPHPQQNGSRRTPAPSRPQLATSSASSPHPKPPPLSQSPPPTTAGPAFRPPGAQLLSEFQSGLPRAGQPFKCVAVDCEMVGTGPMGKRSELARCSIVGHDGDVIYDQYILPVNRVTDYRSRWSGIRPHHLRQATPFQVAKKQIMKLLQGKVVVGHAIHNDFKALEYFHPPALTRDTSRIPLLNARAGLPAAATPSLKNLTRAILSRDIQVGRKGHSSVEDARATMELYKTVEVQWESALARKSSATQDAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNW0</accession>
    <name>W5MNW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027311">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027317">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNW0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010069"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010081.1">
        <property type="protein sequence ID" value="ENSLOCP00000010069.1"/>
        <property type="gene ID" value="ENSLOCG00000008291.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161287"/>
    <dbReference type="HOGENOM" id="CLU_005350_2_1_1"/>
    <dbReference type="InParanoid" id="W5MNW0"/>
    <dbReference type="OMA" id="ELRWHAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008291">
        <property type="expression patterns" value="Expressed in bone element and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="88"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="51758" checksum="F76632DF923A40B7" modified="2014-04-16" version="1">MENVSAPTDPDCDSGSGSDAISQLSTGVPARETDRFGFILGTGLSSDCEGPPPEVIRQREVKWLEIISHWDRFLLKKSSKVKSQCRKGIPPSLRARCWPLLCAATAKMSTYNKIYEHAFCDSPHYTSSISVKERDTDDKLFTSLSSGKDAHTWDGLFSAVKLMNSSCFTRLTRLVCVSTPEICIHWFAHSLCLALTFCVNTIKMIECFPNGGGDTAIPLSLQEGVLFDSRLFSSVLRRACPVADKHLRKHGVEPLMYLTDWFMCLYTRNLPFNTLLRVWDIFFSEGAESEADFQSVKENQSRDTANQREAGQESLCVQGQEGLQSQSESDVGTDVMDLRGTEADVLTPETNLTLPEPSCDSEVNPLPTTQEQSEPRDPKTRREREEEKARKKREKEMEKERQRLERDRERERRKPQTRGKSFQVHSKCSHNMPPPGNKTTDNLVSKRNSAPYFDTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPB2</accession>
    <name>W5MPB2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fibrillin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MPB2">
        <property type="interactions" value="427"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010221"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010234.1">
        <property type="protein sequence ID" value="ENSLOCP00000010221.1"/>
        <property type="gene ID" value="ENSLOCG00000008383.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183158"/>
    <dbReference type="HOGENOM" id="CLU_000233_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPB2"/>
    <dbReference type="OMA" id="NITHRIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008383">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000023">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000044">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000058">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000071">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000086">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000087">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000097">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000131">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="2880" mass="313231" checksum="1E4A780019EDD1E1" modified="2014-04-16" version="1">FVLWGLGSVALSALSVLTVTGQIPANHEKDQFKADEEKISALSRVRRRGQQDFLRGPNVCGSRFHSYCCPGWKTLPGGNQCIVPICRNSCGDGFCSRPNMCTCSSGQIAPTCGSKSIQQCNTRCMNGGSCAEDRCHCQKGYTGTYCGQPVCEDGCQNGGRCIGPNRCACVYGFTGPQCERDYRTGPCFMQVSNQMCQGQVSGIVCTKTLCCATIGRAWGHPCETCPAQPEPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSYECKCPKGHKQNERTQKCEDIDECSTLQGVCDGGQCINTVGGYSCICPPGYMQSADGTRCFDRRGGTCFSSLVNGRCAQELTGLLSKMRCCCELGRCWALGSVPEMCPVRGSDEYRRLCIQGIPLGGFPEVDPGITGGRINHFVPSELDPTITGERVQTGQLHRPLPDFGEFKACIEKETLNQTVDICQRFTNLCLNGRCIPVPSSYRCECNMGFRQDVRGECTDIDECSSNPCTNGDCVNTPGSYYCKCHSGFQRTPTKQACIDIDECLQNGVLCKNGRCVNTDGSFQCICNAGFELTPDGKNCVDHDECATTNMCLNGMCINEDGSFKCICKPGFTLAASGRYCIDVDECQTPGMCMNGRCINTEGSFRCECLAGLAVGVDGRFCVDTHMRSTCYGAIKKGVCVRPFPGAVTKSECCCANSDHSFGEPCQPCPARNSAEFQALCGSGIGFSVEGRDINECALNPDICPNGVCENLRGSYRCICNSGYESDETGKNCVDIDECSVNRLLCDNGLCRNMPGSYSCTCPKGYVFKPDSETCEDINECESSPCANGICRNNAGSFVCECSAGSKLDSTRLICVADSLKGTCWLNIQDGRCEVNINGATLKSECCATLGAAWGSPCERCEIDTACSRGFARSKGIVCEDVNECEVFPGVCPNGRCINTKGSFKCECPEGLTLDGTGRLCVDVRLEHCYLTWDEDECTLPIPRKFRMDVCCCSIGAAWGSECEACPQPGSREYEALCPRGPGFANRGDILTGRPFYKDIIECKVFPNICTNGKCQNTIGSFRCRCDSGFALDTEGRNCTDIDECRISPDLCGSGTCVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPMLCRGGSCENTEGSYRCICPPGHQLSPGGDACIDINECDLSDSLCRNGKCVNVIGTYQCSCNSGYQATPDRQGCVDIDECTIENGGCDTHCTNSEGSYECACSDGYSLMPDHRSCTDVDECEDNPDICDGGQCTNIPGEYRCLCYDGFMTSMDMKICVDVNECDLNPNICMFGDCENTKGSFICHCQMGYSVKKGTTGCTDVDECEIGAHNCDLHASCVNVPGSFHCACQEGWVGNGIKCVDTDLDECANGTHQCSPSAKCLNIPGSYRCACAEGFSGDGYTCSDVDECAENVNLCENGQCLNTPGTYRCECEMGFTPAPDSKSCQDIDECTFQNICVFGTCKNLPGMFRCICDDGYELDRSGGNCTDIDECLNPISCVNGLCKNTAGSYECNCPPDFELNPTGVGCVDTRVGNCYLEVGFRGDNGLSCSVEIGVLVSRSSCCCSLGRAWGNPCEHCPAVNTTEYNTLCPGGEGFRTNPITILLEDIDECQELPGLCQGGNCINTFGSFQCECPSGYYLNDESRICEDIDECITHPGICGPGTCYNTLGNYTCVCPQEYMQVNGGNNCMDMRKSLCYRNYNGTACENELAFNITKRLCCCAYNVGKAWNKPCETCPTPGTSDYRTLCGNGFPGFYFDIETGKAVDIDECREIPGICANGVCINQIGSFRCECPTGFSYNDLLLICEDIDECSNGDNLCQRHADCINSPGSYRCECSDGFRLSPNGACIDRNECLEIPNVCSHGECRDLLGGYQCVCHNGFKATPDRKMCMDVDECERQPCGNGTCKNTVGSYNCLCYPGFELTHNNDCIDIDECRSSYGQLCRNGQCINSIGSFQCLCEEGYELTPDGKNCIVKTDINECLTLPGTCSPGTCQNLDGTFRCLCPPGYIVHNDNCIDINECDEDPNICLFGSCFNNLGSFECVCRPGFVLSESGRRCYDTRESFCFTKFENSMCSAPQPFNTTKAKCCCSKMPKEGWGDPCELCPKEHEDAVAFQELCPFGYGTIPGKEDTREDVNECAENPGICYNGHCINTDGSFRCECPMGYTLNYTGVHCVDIDECSVGNPCGNGTCTNVIGGFECSCEEGFEPGPMMTCEDINECGQNPLLCAFRCINTYGSYECTCPAGYVLRDDRRMCKDLDECSEGFHDCESRGMACKNLIGTFMCICPVGMVRQPDGEGCQDENECRTKPGICENGRCVNTLGSYICDCNDGFQSSATRTQCIDYREGFCFTEVLQTMCQMSSSSRNPTTRSECCCDGGRGWGNQCDLCPLPGTGPYKKLCPHGPGYTTNGTDIDECRVMPNLCKNGQCINTMGSFRCHCKQGFTSDISGTSCVDMDECVQSPKPCNFICKNTEGSYLCSCPRGYILHEDGRTCKDLDECQTKQHNCQFLCVNTIGGFTCKCPPGFTQHHTACIDNNECASQPSLCGARGICQNSPGSFSCECQRGFSLDSTGLSCDDVDECVSNHRCQHGCQNMLGSYRCSCPQGYIQHYQWNQCVDENECSNPNTCGAASCYNTLGSFKCACPSGYSFDQFTTSCEDVNECSYFKNPCSYGCSNTEGGYLCGCPTGYYRVGQGHCVSGMGFGKGQYLPLDGEGDEENALSPEACYECKINGFGKKTGRKRRSSNQTALAEDQPISLASLDIESPLNLNLKISELNPKEHILEVIPAIKPLTNHVRYVISSGNEDGFFHIQQRGGLSYLHTTKKKTWPGTYTLEITSIPLYKQKDLNKLEDSKDRDYMTGELGENLRMRLQIELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQP9</accession>
    <name>W5MQP9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S31, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQP9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010708"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010723.1">
        <property type="protein sequence ID" value="ENSLOCP00000010708.1"/>
        <property type="gene ID" value="ENSLOCG00000008798.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSX9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010017"/>
    <dbReference type="HOGENOM" id="CLU_052666_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQP9"/>
    <dbReference type="OMA" id="EGYDNYP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008798">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026299">
        <property type="entry name" value="MRP-S31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13231">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13231:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15433">
        <property type="entry name" value="MRP-S31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="344" mass="38845" checksum="1E9212BF2A443A14" modified="2014-04-16" version="1">ELVHKLQVVAFSTTTAISKEREEASGPEEVQSTTSEKEQGKSGKESLLDLLGAMKVEVTTKKKFRGLNVQKDAPGALESATSMFQKATSHIESQSVTLSPELVAAASAVASSMPNKRQAESELLQQLRKHESVSDVQRKGQTKNIGNIIADMKIGKRPNIRASARAANQIHFDDDGRGYIHDRGVNSNLEGVRNRKGLFVGKRLNIFQVPPDTAELEPRDSSPSLWDMELANQIAAVTKHPPRNGFEEMIQWTHDGKLWRYPINNEEGLEEEADIPFHEHVFLEKHLEDFPKQGPIRHFMELVITGLSKNPYLTVRQKTEHIAWFREYFQQKEDLLKEAEVYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQU0</accession>
    <name>W5MQU0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iroquois homeobox 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213086.1">
        <property type="nucleotide sequence ID" value="XM_015357600.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQU0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010749"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010764.1">
        <property type="protein sequence ID" value="ENSLOCP00000010749.1"/>
        <property type="gene ID" value="ENSLOCG00000008834.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684613"/>
    <dbReference type="KEGG" id="loc:102684613"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160429"/>
    <dbReference type="HOGENOM" id="CLU_042927_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQU0"/>
    <dbReference type="OMA" id="QVNHTST"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008834">
        <property type="expression patterns" value="Expressed in heart and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF13">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="125"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="127"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="379"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="461" mass="50697" checksum="F5628645B198275F" modified="2014-04-16" version="1">MSFPQLGYPQYLSATQAVYGGDRPGVLAASARGGSTDIGANPTATAAAVTSVLGMYANPYAAHNYSAFLPYTSADLALFSQMGSQYELKDNPGVHPASFAAHTSPAFYPYGQFQYGDPARPKNATRESTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKVTWGTRSKDDEDGNIFGSDNEGDAEKNEDDEEIDLESIDIDKIDENDGDQSNEEDEEKLETRERREIDSMEKRRALTLQAHEGFDKSKDTISTGKDPSESNNTRVLSPGGQSNLQLPANNKPKIWSLAETATTPDSAQKPTSPSAPVSHASPQIQHPAFLPSHGLYTCQIGKFHNWTNGAFLSQNSLLNVRSFLGVNQHHHHHNHSLHAQQQQSSVVASSVVALNSEKSSESLSPKHIERENVQRTESPTQPLKSSFQPVHDSPRNQQEATQRVLTALSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR36</accession>
    <name>W5MR36_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkey-152b24.8</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069041113.1">
        <property type="nucleotide sequence ID" value="XM_069185012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR36"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010845"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010861.1">
        <property type="protein sequence ID" value="ENSLOCP00000010845.1"/>
        <property type="gene ID" value="ENSLOCG00000008906.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696153"/>
    <dbReference type="KEGG" id="loc:102696153"/>
    <dbReference type="eggNOG" id="ENOG502QT3N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010118"/>
    <dbReference type="HOGENOM" id="CLU_024117_3_0_1"/>
    <dbReference type="InParanoid" id="W5MR36"/>
    <dbReference type="OMA" id="YGANDIM"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008906">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08586">
        <property type="entry name" value="PI-PLCc_BcPLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF147">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL PHOSPHODIESTERASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866412" evidence="1">
        <location>
            <begin position="34"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="2">
        <location>
            <begin position="56"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="313" mass="35271" checksum="117C0B298BEDA335" modified="2014-04-16" version="1">MIMRPKSSIIWSPKKIPLDVCLLLLLLAINCSGNRNFFDDTEAPEFNNEDWMKEIPDNTLLSAVNIPGTHDSLSLYCTRLAICQSWTLDKQLQAGIRFLDVRIQCFPLCGDPVICHGITPQFDWFSNVVKVVMNFLQAHKKETVVMRLKAELCETGVNTLVKVKNMLNNNGAAGALWESTDVPKMRQVRGKLVILQEFSGETQWIKYDSLDIGDDYQVQNINEKWTSVQNHLNSAIQGDKEKLFLTFSSGTGISATQIICALCPHHVAQTINNKLFDYLPTLASNKNRLGIIAMDFPSAQLIQKIIELNQQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRH4</accession>
    <name>W5MRH4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside-triphosphate phosphatase</fullName>
            <ecNumber evidence="3">3.6.1.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01003992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRH4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010983"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010999.1">
        <property type="protein sequence ID" value="ENSLOCP00000010983.1"/>
        <property type="gene ID" value="ENSLOCG00000009002.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286963"/>
    <dbReference type="InParanoid" id="W5MRH4"/>
    <dbReference type="OMA" id="QYDVMEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009002">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24045">
        <property type="entry name" value="ASKHA_NBD_NTPDase4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000057">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000003">
        <property type="entry name" value="ectonucleoside triphosphate diphosphohydrolase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF37">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="566"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="276"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="613" mass="69621" checksum="439C96E6E982E3A2" modified="2014-04-16" version="1">FCRLSFSCLLPASWHFSVSLFSLGVSLRQCLLFLAWLLTLSHMLNNSEPLQINVLQVKDDLHSKRSFCPSHRYLSIVETMEATDVANPSLNFGVVVDCGSSGSRVFVYYWPPHNGNPHSLLDIRQMRDHSRTPVVKKIKPGISTLALTPDKASDYLQPLLSFAAGHIPRKKHKETPLYILCTAGMRMLPESQQEAILEDLITDVPVAFDFLFSSSHAEVISGKQEGVYAWIGINFVLGRFDHAEEGEDAVVKVSPGTQNQQPISRKRTVGIIDMGGASLQIAYEVPSAITFTSPEQEEAAKSLLAEFNLGCDVEHTQHVYRVYVTTFLGFGGNMARQRYEDLLVNSSYSRSRLTGDQTGMSVSTPYPEPCLPVGLTDSVHRGGQTVFLRGHGDWLKCQEVVRPFLEESNNNISLAGVYQAPIDFHNSEFYGFSEFYYCTEDLLRIGGLYDSTKYTRAATDYCATKWSILKQRFKSSLYAPQADLSRLKYQCFKSAWMYEVLHSGFKFPKDYTSLKTAQLVYDKEVQWTLGAILFKTRFLPLRDLQQEAFRPSHSSWLRFSFVYNHYLFFACILVVLLAIFLYILRLRRIHQRELRQAETLDLLWVEEGEPLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRS7</accession>
    <name>W5MRS7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRS7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011086"/>
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        <property type="protein sequence ID" value="ENSLOCP00000011086.1"/>
        <property type="gene ID" value="ENSLOCG00000009092.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S0E1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166985"/>
    <dbReference type="HOGENOM" id="CLU_008338_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRS7"/>
    <dbReference type="OMA" id="CGDGENI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450:SF2">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="31055" checksum="277DC4D42BF3DBE2" modified="2014-04-16" version="1">AVPMPQYSFGMIDWRQQKIQDMERKTRKLLTKYGLLHPKADVQYIPRRSGGHGLTQLKTAYKSTIDGLGKYIKRGSNPEISSHTTTSNWESIKGRMKSSLIEEKINKLLSKPLHSQFYKQTFNNYKTAFGWLQNSGLKGQTESYHALSTRYHQSNILKMDVDGKCRLCHQQAEHISNIVARCSLLTPKEYTHRHNRIASYLHWSMLQELGLPVPDHWYDHQPENVVESCGDGENITIMYDMAVNTNHKTGANRPDIILHDLSKKRCFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MST6</accession>
    <name>W5MST6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Actin binding LIM protein family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABLIM3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027431">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027433">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027436">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MST6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011445"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011462.1">
        <property type="protein sequence ID" value="ENSLOCP00000011445.1"/>
        <property type="gene ID" value="ENSLOCG00000009374.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="HOGENOM" id="CLU_001357_12_3_1"/>
    <dbReference type="InParanoid" id="W5MST6"/>
    <dbReference type="OMA" id="NNHFHIQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009374">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09328">
        <property type="entry name" value="LIM2_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09329">
        <property type="entry name" value="LIM3_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000003">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000007">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000024">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000001">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF0">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="151"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="11">
        <location>
            <begin position="618"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="373"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="686" mass="77805" checksum="3E0FCE41F914394B" modified="2014-04-16" version="1">MSGGAAPYQQSSYSGGRSTSAIHCFRCRDVCKGEVVRVQNVHFHVKCFTCQVCGCDLARSGFFQKNGEYICTADYQRLYGTKCDSCGDFITGEVVSALGRTYHPKCFVCSVCRKPFPIGDRVTFSGKDCVCQQCSHTLVNTNEPIKIHGPSHCAGCKEEIKQGQSLLALEKQWHVSCFKCQTCGIVLTGEYISKDGVPYCETDYHTQFGIKCETCNRYISGRVLEAGGKHYHPTCARCARCHMMFTEGEEMYLTDSDCYIDRTRTDSISSLLQHRRTSETSISPPGSSIGSPSRVICAKVDNEILNYKDFAALPKVKAIYDVQRPELISYEPYHRYTSDDRLERYSYGEVMHTVKPKIQDIYDSIDLRQRRASSPGYIDSPSYSRQGMSPTVPRSPQHYYRSGTESGRSSPYYSQVDVRSSTPTAYQAPKHFHIPATGDPNIYRKPPIYKRHGTASDCDESMFNESRYPGLKKSSGFQHKPLPQPVYTQTPKNALTAPRARRFSSGGEEDGWNHSLNRIQGGIGRMILKEEMKARSGSYDNDPWASARNSRSGSKETLLGLGYETSLNGSPRSHYTVDNVLDFTEGQISWYVRSLKLFQPQSADYFQYDSSNAVNWGIREYKIYPYETLIVTTRGRNRLPKDVDRARLERHLSPEEFYQVFGMSIVDFDRLALWKRNELKKQARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSU2</accession>
    <name>W5MSU2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box I2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSU2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011451"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011468.1">
        <property type="protein sequence ID" value="ENSLOCP00000011451.1"/>
        <property type="gene ID" value="ENSLOCG00000009392.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166929"/>
    <dbReference type="HOGENOM" id="CLU_046860_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSU2"/>
    <dbReference type="OMA" id="AGYLWGM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009392">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF383">
        <property type="entry name" value="FORKHEAD BOX I2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="130"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="130"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="381" mass="41610" checksum="C954359F29388264" modified="2014-04-16" version="1">IMNSIDSQAHHSSPAPSPHNPHLKSTQEAPEMAVYCDNFSMYHQQSLHSTQRPGGYGLGDYAPSSNPYLWLNGPGVNSSTSYLHGNNSASFMPPSYGSQRQFLPNSSGFGGADLGWLSIASQEELLKLVRPPYSYSALIAMAIQNAPEKKLTLSQIYQYVADNFPFYKKSKAGWQNSIRHNLSLNDCFKKVPRDEDDPGKGNYWTLDPNCEKMFDNGNFRRKRKRRSDSSSGAPANAKPDESRSNPTVKPADSPTLLEPSSPELDTATEDHKSSSPPGLPSAPCFNSFFSSMTGLGSGSVNRQGSLGLVNELSARNISALSPYPANSQQESGSAELTDSLQLGRSVYYNSFSSGQGNQFNGHFYNSFSVNSLIYPREGTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT11</accession>
    <name>W5MT11_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Somatostatin</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Somatostatin inhibits the release of somatotropin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the somatostatin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637937.1">
        <property type="nucleotide sequence ID" value="XM_006637874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MT11"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011520"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011537.1">
        <property type="protein sequence ID" value="ENSLOCP00000011520.1"/>
        <property type="gene ID" value="ENSLOCG00000009439.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694961"/>
    <dbReference type="KEGG" id="loc:102694961"/>
    <dbReference type="eggNOG" id="ENOG502S11K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047914"/>
    <dbReference type="HOGENOM" id="CLU_124515_1_1_1"/>
    <dbReference type="InParanoid" id="W5MT11"/>
    <dbReference type="OMA" id="AEQDDMR"/>
    <dbReference type="OrthoDB" id="9948948at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009439">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004250">
        <property type="entry name" value="Somatostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018142">
        <property type="entry name" value="Somatostatin/Cortistatin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558:SF2">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03002">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001814">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866518" evidence="9">
        <location>
            <begin position="25"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Somatostatin/Cortistatin C-terminal" evidence="10">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001814-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="114" mass="12470" checksum="924F8D3A3F0A4C97" modified="2014-04-16" version="1">MLSSRVQCALALLCLALAVSSVSAAPSDLRLRQLLQRSLAASAGKQELARYTLAELLSELAQSENEALESDDLSRAAEQDEVRLELERSANPALAPRERKAGCKNFFWKTFTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTL5</accession>
    <name>W5MTL5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTL5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011724"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011742.1">
        <property type="protein sequence ID" value="ENSLOCP00000011724.1"/>
        <property type="gene ID" value="ENSLOCG00000009598.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="HOGENOM" id="CLU_000384_6_0_1"/>
    <dbReference type="InParanoid" id="W5MTL5"/>
    <dbReference type="OMA" id="HITHRTT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="13"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="30303" checksum="1AD46259127C4064" modified="2014-04-16" version="1">PTRRATAPPQQHQVGAPMEWVGVDVLAPFPRTEAGNCYVLVAMDYFTKWPEAYALPDQSAPTVADALLERMFARLGEVFARVCQHLGITKTRTTPLYPQSDGLMERFNRTLATQLATLVSRPQRDWDRHLPVALWAYRTAVQESTGCTPASLMLGRDMRTPVDLVFGPPPGDGSAPLAGLDYKWDLRQRMRCVHSFAWTHLTQAGVRGPAFQHGDWVYSPHCRKGLRPKLAPSWEGPAEVLGVVGEVCYRVRLRTRGRVVVLHRDRLAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTQ6</accession>
    <name>W5MTQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aldehyde dehydrogenase 1 family member A1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTQ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011765"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011783.1">
        <property type="protein sequence ID" value="ENSLOCP00000011765.1"/>
        <property type="gene ID" value="ENSLOCG00000009624.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154609"/>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_1"/>
    <dbReference type="InParanoid" id="W5MTQ6"/>
    <dbReference type="OMA" id="KTYMGPL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009624">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018479">
        <property type="term" value="F:benzaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="44"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="519" mass="57213" checksum="03D51B7F71071CD7" modified="2014-04-16" version="1">MTTAPGDSQNHEPSSGAAQQDSGFPLPVHDMEIKYTKIFINNEWHESSCNRKIQVYNPATGEKICEVEEGDKEDIDKAVKAAKEAFQLGSPWRRMDASERGQLLNKLADLVERDRQILATLEAIDSGKVFLVAYFVDLTAAIKTLRYYGGWADKIHGQTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTIIMKPAEQTPLSALHMAALIKEAGFPQGVVNIVPGYGPTAGAAIANHMEIDKVAFTGSTEVGKTIKTAAGNSNLKRVTLELGGKNPNIVFADADLDHAVEQAHHGLFFNQGQCCLAGSRVFVEESIYEEFVCKSVERAKKRVLGNPLVPGVDQGPQIDKKQFEKILDLIESGKKEGANLECGGSFWGEKGFFIQPTVFSDVTDDMRIAKEEIFGPVQQIMKFKTMDEVIKRANNTHYGLVAGVFTKEIAEKKISSTMTRAGAKWIHRVNCYNAMSSQSPFGGFKMSGNGREMGEYGLQEYTEIKTITIKIPQKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU82</accession>
    <name>W5MU82_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Heterogeneous nuclear ribonucleoprotein A/B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APOBEC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU82"/>
    <dbReference type="FunCoup" id="W5MU82">
        <property type="interactions" value="1492"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011941"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011962.1">
        <property type="protein sequence ID" value="ENSLOCP00000011941.1"/>
        <property type="gene ID" value="ENSLOCG00000009773.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154735"/>
    <dbReference type="HOGENOM" id="CLU_012062_1_1_1"/>
    <dbReference type="InParanoid" id="W5MU82"/>
    <dbReference type="OMA" id="NQGYNYW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009773">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12757">
        <property type="entry name" value="RRM1_hnRNPAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000186">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein A/B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012956">
        <property type="entry name" value="CARG-binding_factor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034846">
        <property type="entry name" value="hnRNPAB_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF1">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08143">
        <property type="entry name" value="CBFNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="87"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="171"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="359" mass="39709" checksum="E7C24C66C5FE5A7B" modified="2014-04-16" version="1">HTHRLSPSPLCCSSPLDNDQDHTTMSDTEQQFMETSENGHEGEEDLNGAEPAEQAAAAAESTAEGDSQNGASEGGQINASKSEEDAGKMFVGGLSWDTSKKDLKDYFSKFGEVTDCTIKMDPNTGRSRGFGFILFKEAASVEKVLEQKEHRLDGRQIDPKKAMAMKKEPVKKIFVGGLNPETTEEKIREYFGTFGEIESIELPMDPKTNKRRGFVFITFKEESPVKKVLEKKYHNVSGSKVTNGKDDLCEIKIAQPKEVYQQQQFGGRGGGYGGRGRGNRGGQNQNWNQGYNNYWNQGYGNQGYGYGGQQGYGGYGGYGNYDYTGYYGYGQGGYDYNQGNTSYGKTPRRGGHQSSYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUC4</accession>
    <name>W5MUC4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tectonin beta-propeller repeat containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MUC4">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011983"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012004.1">
        <property type="protein sequence ID" value="ENSLOCP00000011983.1"/>
        <property type="gene ID" value="ENSLOCG00000009804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695464"/>
    <dbReference type="KEGG" id="loc:102695464"/>
    <dbReference type="CTD" id="9895"/>
    <dbReference type="eggNOG" id="KOG3621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157283"/>
    <dbReference type="InParanoid" id="W5MUC4"/>
    <dbReference type="OMA" id="WFRTGVC"/>
    <dbReference type="OrthoDB" id="9930272at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009804">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032527">
        <property type="term" value="P:protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056499">
        <property type="entry name" value="Beta-prop_HPS5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287">
        <property type="entry name" value="RUBY-EYE2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287:SF16">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23756">
        <property type="entry name" value="Beta-prop_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06462">
        <property type="entry name" value="Hyd_WA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19193">
        <property type="entry name" value="Tectonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPS5-like beta-propeller" evidence="2">
        <location>
            <begin position="36"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1466" mass="159424" checksum="B2BF60C3A8D6D44A" modified="2014-04-16" version="1">MAAQASPAALKEFCPLYYLLNTIPAKVQRGFRSVLVYLTALDASSDYIAVGSSIGMLYLYCRRLNQMNKYNLEGKTEAITSVKLLSCFDDLVAVGTASGRVALFQLVSPLPGRNKQLRRFDVVGLHKSTVTALAWSANGMKLFSGDDKGKVVYSAIDLDQGVCNPSVLLEEQSAIVQLEYSQKVLLVSTHQHSLLFYTEEKAVQQVGTKPRKGNGKFGACFHPGLCKQSDLVVYAARPGLRLWKADVQGSVQATYILKDVFAEGVRPFELFPRAGSHATEGYRPAERQLGLLSCFLREGWVLSWNEYSIYVLDSLNQAVVGGLEGSGDIVSVSCTDHEIFLLKGDRDIIRISSRPEGLASSASDVPLWLSTPTATAPQMVQPTGAVETAQPIRTMSIIAESRDTQPDGEEDCRREGGRKEQDAVIVLEKIEVALGDPKGAPQREQASEAPLRGGSGTSESRSRSSSVTSWDSALGLGAAVEQSASELSVEGLSSSTRFSTVSTEDFEQELVVKSIRVRKKKKKKRHDSGNRLSDHSSWSEGVFSQDAVSDGTTTPVCELLSDRTSLLGSSLDLQSADSPDREGSVSGDSHSHDLFAVLTSPEVTCTRLGEPPSADLRDLPAEVLGLEAPVPQSCETSDVPLAKAPSSSPPSPRLLESDGQEVTQKDPLALQSESNRYALPGQGAKVRGSIPDCDADALLECNSLNTQASAERVSGEDGLVRSEKDESEPHVPPSHTHDLSTLDDFGLLGVGGSLEASDQNLVTPFPALHDVISHVSTLSTRAQEQEWQDAGLQCISSDEEDIYAHAVPHSASDASLVEQPLLGTNLQRLIDGHCSDAAKSDLEDSSLFKSDQFAESWMGYSGPGYGILSLVVSEKYIWCLDFKGVLYCSPLPSAGLRWQKFEDSVQQVAVSPSGSLLWKVEQKTNKAFACGKVTIKSRRHWYEAIPQTAFVALSDDTAWIIRTNGDLYLQTGLSVDRPCARAVKVDCPCPLTQITARGSVVWALTEQRGVFYREGLSSFCTEGEQWKCDAVSESQGLEPVCIALGDHNTAWALDTSGNLWFRTGITAQKPQGEDDHWWQVSITDYVVFDQCSLFQTIIQATHSVASAAQAPVEKVADRLRVAFWSQQPQCQPSLLGVNSSGVWIASGRNDFHVAKGSLIGTYWANVVPRGTASAAKWAFVIASSAPTKEGSFLWLGQSCKDLFCISDQCPQFRPSTVQLPPEVEMVHLSACQDAVWGLDTRGRVYIRTLSKSCPTGMHWTPLDLSQLGRVKLSSLSCGSQNVWACDANGMVYFRVGTQPLNPSMMLPAWISIEPPEQPVGVHLVSVYTSPNDRMLWAVDSRGYVHVRTGITEEMPVGTDWEHVPGLQAWQLAVSARTVWVRCPNGDVARRYGITEKNPAGDYWKKIPGLVSCLTVTPVDELWAVGMTGSLIRRLTKAFCHSNSRSKVSMASSSLNGDDPEDEWEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUC5</accession>
    <name>W5MUC5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">CSN12-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUC5"/>
    <dbReference type="FunCoup" id="W5MUC5">
        <property type="interactions" value="1589"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011984"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012005.1">
        <property type="protein sequence ID" value="ENSLOCP00000011984.1"/>
        <property type="gene ID" value="ENSLOCG00000009805.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001101"/>
    <dbReference type="HOGENOM" id="CLU_031567_2_0_1"/>
    <dbReference type="InParanoid" id="W5MUC5"/>
    <dbReference type="OMA" id="INRMFTL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009805">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000146">
        <property type="entry name" value="PCI domain-containing protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="213"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="402" mass="46321" checksum="BBDAE3F0834E03B5" modified="2014-04-16" version="1">ISSMAHITINQYLQQVLEATDTRDGAFCAELLSFKHPHVANPRLQLPSPEEKCQQVLEPPHDEMVAAHLRCTYAVANHDFVEAYKCQTVVVQSFLKAFQAHKEENWALPIMYAVGLDLRIFANNAEQQLAKKGKGKVGDMLEKAAEQLMSCFRICASDNRAGIDDSKKWGMLFLINQLFKIYFKINKLHLCKPLIRAIDSSNLKDEYSIAQRVTYKYYVGRKAMFDSDFKQAEEYLSFAFHHCHRSCQKNKRMILIYLLPVKMLLGHMPTHQLLRKYDLLQFADVTKAVSEGNLLLLNEALVKHETFFIRCGIFLILEKLKIITYRNLFKKVYLLLKAHQLPLDVFLVALKFMQVEDVDIDEVQCILANLIYMGHIKGYISHQHQKLVVSKQNPFPPLSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUD0</accession>
    <name>W5MUD0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NIPA like domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004717">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUD0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011989"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012010.1">
        <property type="protein sequence ID" value="ENSLOCP00000011989.1"/>
        <property type="gene ID" value="ENSLOCG00000009809.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159321"/>
    <dbReference type="HOGENOM" id="CLU_012349_2_0_1"/>
    <dbReference type="InParanoid" id="W5MUD0"/>
    <dbReference type="OMA" id="HLQQSFI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009809">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF16">
        <property type="entry name" value="NIPA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="351" mass="38367" checksum="4243F6FCACBAB5FD" modified="2014-04-16" version="1">TPFVSDIGQQFQGNTTLCESVQESCQQTQLLGILISIIGNFLISISLNIQKYTHVRVSKCPDSKPYYKSALWWCAIILMGIGELGNFTAYGFAPATLIAPLGCVSVIASAVISVIFLKETLRASDILGGALAVSGTYLLITFAPSVAQKITARVVQKYSVSWHFLIYICLEIIVFCILLYLHEKKNVKHIVVVLMLVALLASLTVISVKAVSGMITESVKGSLQLTYPIFYVMTVVMVASCAFQIRFLNQAMKLFNATEVVPINFVFFTASAIIAAGVIFYQEFYGLALLNIFMFLFGCLLSFLGVFLIARNRPESKTEHPFIDIGQVPGKTSTDKIQPETGGTSYGALDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW03</accession>
    <name>W5MW03_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SH3 domain and tetratricopeptide repeats 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MW03">
        <property type="interactions" value="1133"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012562"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012586.1">
        <property type="protein sequence ID" value="ENSLOCP00000012562.1"/>
        <property type="gene ID" value="ENSLOCG00000010260.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694600"/>
    <dbReference type="KEGG" id="loc:102694600"/>
    <dbReference type="eggNOG" id="ENOG502QUY4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063812"/>
    <dbReference type="InParanoid" id="W5MW03"/>
    <dbReference type="OMA" id="FTNEQQG"/>
    <dbReference type="OrthoDB" id="9927874at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010260">
        <property type="expression patterns" value="Expressed in mesonephros and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042772">
        <property type="entry name" value="SH3TC1/SH3TC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647:SF3">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEATS CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="263"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1163"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1305" mass="149085" checksum="423ACB52BAECABC0" modified="2014-04-16" version="1">MSSALGELPEAKQVRNGGEVNFRDPPWIQELDAMESGFGNKSKDAAIFMKLVMVRRKTGLPDEELQEMLRGKLRILENDSGEVMALFSELSARLLSIHSEEDIIVVTFKTFEEIWKFNTYYSLGLLGHCMENLLLDQVFWLHHLDEEDLGIEVLIHEETLHLMYKGILMQEESFFAVCTAEQNCESASTGSELRLQEGNVAIFEPPSDGSEWTVQLLVDGSRGSIAKPALEPVSPFHQWFLKACPENILMGNAKLLCEFPYQFAVGSCIATVGYEGSGPDELSFKSGDCVEIVGFLVSCFEWFVGKHSETGRTGLVKTSLVKPTNSICESAEVFLSEEEESLFKWKEEFFEEKSIDVLKKMCQTDVDAVYKLDKLDTQDPPGIPRQTSTFNNESDPQRQELQNKIAVCLSRLKEPHVPPKETQHEDRQSEHHHNHSLSSKEHSEPCFIINSEGDNHSADDFQTLLAFLNCSEYKEEFRNIYDFSFRFLQSMFQGYTDDEELVFYLGMARENARKKKFLWAQTRICFLLGKLCARRLKFSQARVYFEEALSVAREQFKDIFLLISIYTNLAAIYLKQKNKEKYVAIFERIAAFLMGIPNYICSTEQESEILKYVLKKAVLAQNKVAEVRTCFLLAKLYTKLNQPQSALLFTERLQILAKELSLTSKVMPSDYYLTLGKLYGAKYLPHLAVSSVKQAVFHTGATLTDCLIGTEFVMDNASRLYSLGKCGVLVPTQVAPYLNKALYFAADKKDQVLCQTISLCLSHLHQYHGMLNKAIWYIDTVISKNVWPSATHAANILIHLAWLHILNRQHHMALDILTSGLGSLSEHCTKLQKGLVYNMRAVALRYTEDIKQAAESYGKAIQICEESDFKYNRAIAQANFGFLCLHAKAKGLAELSLVRAVELFSQLEDVAHEENFITVLLELGQYYVSQTQLEKGMICYEWAFLMAIKANHVECQLQATKQLCHFYHMICPNEAQCIIYNEYMLTLVRKAGNKEWEGQILETISQLYFSLGTERAYKSALDYTKRSLGIFIDLRKKEKEAYAWLQAGKIYHILRQNELVDLYIQVAQDCCLSTADMHFVLELLEAAGDVFFNSSQDREKALTFYRDKALPIALKTGNVKTQLRLSNKLAELLLQLKFYGEAVDHAQAALAISISLGERLNERVAFHRLATLFHCLGQYEMAEHYYLKALSLCPSPLEFDEETMYYVKVYQLLGDMIFYDLKDPYDAAGYYHLALAAAMDLGNKKSQLKLCTRLATIYHNFLINRELSLFFYQKARVFAADLNVRRINLSPDQYYQSVSQYKTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX15</accession>
    <name>W5MX15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Splicing regulatory glutamine/lysine-rich protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Serine/arginine-rich-splicing regulatory protein 86</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Splicing factor, arginine/serine-rich 12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Participates in the regulation of alternative splicing by modulating the activity of other splice facors. Inhibits the splicing activity of SFRS1, SFRS2 and SFRS6. Augments the splicing activity of SFRS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX15"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012924"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012951.1">
        <property type="protein sequence ID" value="ENSLOCP00000012924.1"/>
        <property type="gene ID" value="ENSLOCG00000010547.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110872"/>
    <dbReference type="HOGENOM" id="CLU_030029_2_0_1"/>
    <dbReference type="InParanoid" id="W5MX15"/>
    <dbReference type="OMA" id="VISTREH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010547">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12519">
        <property type="entry name" value="RRM1_SREK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12260">
        <property type="entry name" value="RRM2_SREK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000084">
        <property type="entry name" value="Serine/arginine-rich splicing factor 11 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000142">
        <property type="entry name" value="splicing regulatory glutamine/lysine-rich protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034192">
        <property type="entry name" value="SREK1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF71">
        <property type="entry name" value="SPLICING REGULATORY GLUTAMIC ACID AND LYSINE RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="169"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="280"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="432"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="473"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="543"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="605" mass="67730" checksum="5FD30528F289D56D" modified="2014-04-16" version="1">GIPGTPVIQITNLSPAVSSEQMRTLFGFLGEIEELRLYPPDNAPLPFSSKVCYIKYREPSSVGVAQHLTNTVFIDRALIVVPCAEGKIPEEAKALSLLAPATTVASLIPGAGLLPIPTPAPIPSLGIPIGNLGALQAGLDPTVAALGGIPAQPPLMGNVDPSKIDEIRRTIYVGNLNSQTTTAEQLLEYFKQVGDVKFVRMAGDETQPTRFAFVEFSDQESVPRALAFNGVMFGDRPLKINHSNNAIVKPPELTPQAAAKELEDVMKRVREAQSFISAVIEPESGKSSSTGSKKAAKSRTRSRSHSRSRRKRSRSRHRSRASRRSRSKVRESHGSWSSHKKHSRSRERRHSRSRSRSRKSEQKQAAISTSLCENYVVPWAHQQNTDDGRFPLERSQAPTQDSTLWKEPVKCSYSLRDCTITSFLYNSHFAFSERKKEKESKSKGKEESSKDKEERRQKDKKGKTPPKSYTSSRRSRSPSSRTHRKRSRSRSKSLKRSRTPKRKVKSPSPRSRSKKDKKKERVRERSRERGERERSTSKKKPSKDKEKQEKAVTPIKVDKHSDKEEKEYEGGKDSIKARSGDQAKIQHNGSYSKHEGNPPVKTEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ92</accession>
    <name>W5MZ92_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 104</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TMEM104 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211816.1">
        <property type="nucleotide sequence ID" value="XM_015356330.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ92"/>
    <dbReference type="FunCoup" id="W5MZ92">
        <property type="interactions" value="594"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000013730.1">
        <property type="protein sequence ID" value="ENSLOCP00000013701.1"/>
        <property type="gene ID" value="ENSLOCG00000011150.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695601"/>
    <dbReference type="CTD" id="54868"/>
    <dbReference type="eggNOG" id="KOG3832">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001244"/>
    <dbReference type="HOGENOM" id="CLU_025541_4_0_1"/>
    <dbReference type="InParanoid" id="W5MZ92"/>
    <dbReference type="OMA" id="GHREGHP"/>
    <dbReference type="OrthoDB" id="294541at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011150">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="139"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="492" mass="55215" checksum="EAA1BE691D5F4C69" modified="2014-04-16" version="1">MAGGITDTGEPYSPYVGLVYMFNLIVGTGALTMPKAFATAGWVVSLALICFLAFMSYMTTTFVIEAMAAANAHLRWKRRETEKVINDSDSTSDYSDDEVLARDHAETHPILSVRRGVSPDHFEIVERVEMGQMASIFFNKVGLNMFYICIIVYLYGDLAIYAAAVPISLMEVACGNHSCSAGSSVKYNDTDACWGPVKRKDAYRVFLTIFTLLLGPFTFFNAQKTKYLQIMTSLMRWIAFIMMIILALIRIGKGEGKGHPPMADITGVPNLFGVCVYSFMCQHSLPSLVTPISDKKRMNCLVLSDYVLILGFYSLLSFTAIFCFNNSFLKDMYTLNFTDNCDVIDVAVLRYFLGLFPVFTISTNFPIIAVTLRNNWKTLFHREGGTYPWVVDRVIFPLITLIPPIIVAFCTHDLEYLVGITGAYAGTGIQYIIPACLVFFSRKHLEPVFGQDVVNKHRSPFRHTVWVIFVLVWAISCLIFVTANIILTETKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N018</accession>
    <name>W5N018_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013977.1"/>
        <property type="gene ID" value="ENSLOCG00000011374.1"/>
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        <property type="component" value="Linkage group LG9"/>
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        <property type="entry name" value="TRG_var/const_region"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013977.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="236" mass="26262" checksum="A8C9B24A76BFA378" modified="2014-04-16" version="1">VSVTPSDGTETFTKREGKTARINCKISNVGSCQNYIHWYQKKGEASKRILYVSLSGGDGVSDTNHGGADFQARQNGNERYLQIPKVKDSHSAMYYCACWVSHSFPVKKNQSQTISTLNKKSDHIKDDIYIGSENDSKSEQDKATLTKGSGKTARIACKYKGSGFDSAIIHWYQKKENEAMRRILYIQSVGGTPTYDNVNDKEVFKASKNSNSESELMIVGAQSSHSATYYCACWDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5N063</accession>
    <name>W5N063_LEPOC</name>
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        <submittedName>
            <fullName evidence="16">Protocadherin 12</fullName>
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    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005402">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="651"/>
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        <property type="gene ID" value="ENSLOCG00000011407.1"/>
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        <property type="component" value="Linkage group LG6"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Si:dkey-30k22.7"/>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
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        <property type="entry name" value="Cadherin_CS"/>
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        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF42">
        <property type="entry name" value="PROTOCADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
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        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866959" evidence="14">
        <location>
            <begin position="23"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="710"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="16"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="134"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="243"/>
            <end position="350"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="356"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="462"/>
            <end position="566"/>
        </location>
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    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="614"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="775"/>
            <end position="800"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="819"/>
            <end position="930"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="978"/>
            <end position="1024"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1155"/>
            <end position="1187"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="819"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="894"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="996"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1163"/>
            <end position="1179"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50268"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1187" mass="130915" checksum="DFA09F90FF6720AF" modified="2014-04-16" version="1">MILCAAQLPVLALWLVITVSSSDTIALTIQYKVLEEEPSGTMIGTLADELKVRGETGVLDNFQIVKQALPLPFVVGLQDGMVLTKGQLDRETLCRDTGLCLIAFNVLFNTGGAIGFLQVNVEVLDINDHSPTFPSAEQEIEISETAMLRMRIPLDRALDPDAGPNGLQTYSLSPNQHFALDVITHSDGTKHAELVVIKELDREVQSSFELTLVAWDKGNPPRSGSTLVKINVLDSNDNSPVFEESLLAIDLSEDTVRGTTVINLKATDPDQGANGEIEYSFSKHAPLEVQNLFSIDPKTGTVNLTGELDYEKKHTYEIDIQARDLGPNAIPSHCKLHIKVLDVNDNAPNIHFTWAPQDSAFATVSEGAAKETFLALVMVSDADSGSNGEVHCHIQHGLGHFRLKKVHRDNYMIVTNSVLDRESRVEYNLTLVAKDHGIPPLSVTKHLTVHVLDENDNPPVFSKVLYEASFEENNEPDYLILSVDAHDVDLDLNGKISYSIKESKDSDKFIIHPTTGYIHARQSFDFELFKSFTLIVEAADQGQPSLTSSATVLINIKDINDNFPIIEEPVSKRGRASITIPVNTEKGEIFIELGDIQGDRSSTYLFEYPSTTKIRAHDMDSGLNGMLHYKIIKGNTDGLFLLDQLTGEMYVNSSNATELIGKVFEIDIAVSDLGSPPLVTEANLEITFINLLDHQKNSSPGQRGQLSSTMMLAICLGATFLLLLVVVALVTTFCRTEKRDNRAYNCRQAESTYTRHPRRPQKQIQKTDIQLIPVLRGRKEEALEEDPQKTSIPKEEEIPETQAFFPPCQFNLSPSLSRTLRNQTFPDNNGTLPLHPPRTLRKPSNIEIDGSLPRTPATPYRTLRRTKNSEVQAEAPASPTPSSRLGTLRRQGSPEIQQSSEGTSDSAPSSPPFRTLRRQRNVDCKMKEDKEDHQQILRNLVRMSMAAFAEYSPIELSSASPEVQQISQLLSLLHQGQLQPRPNFRGNKYSHRAGRSGTQDTDWLSTKDSGHGESEAGDVDWDIGRDSPIDPMLGEGLDMLLSNTDDVFADAPDPAWMARLSLPLSADYRENVFVPEGHSSPETLLSPQALQEATTCFSTFGKTSGKGSALPGHLFSEVSTLFEMLLTQKADSQPQTSAEMLYRLSAAYRRSLGLDGTGGGAQQPRSPAAENNSHATYSSGMGLENHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2C0</accession>
    <name>W5N2C0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein Mdm4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MDM2/MDM4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2C0"/>
    <dbReference type="FunCoup" id="W5N2C0">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014779"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014808.1">
        <property type="protein sequence ID" value="ENSLOCP00000014779.1"/>
        <property type="gene ID" value="ENSLOCG00000012014.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQNV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063539"/>
    <dbReference type="HOGENOM" id="CLU_043544_2_0_1"/>
    <dbReference type="InParanoid" id="W5N2C0"/>
    <dbReference type="OMA" id="IPDCRRT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012014">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17673">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16784">
        <property type="entry name" value="mRING-HC-C2H2C4_MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051652">
        <property type="entry name" value="MDM2_MDM4_MUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015458">
        <property type="entry name" value="MDM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016495">
        <property type="entry name" value="p53_neg-reg_MDM_2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183">
        <property type="entry name" value="MITOCHONDRIAL UBIQUITIN LIGASE ACTIVATOR OF NFKB 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183:SF37">
        <property type="entry name" value="PROTEIN MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500699">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006748">
        <property type="entry name" value="p53_MDM_2/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DM2" evidence="11">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="489" mass="54631" checksum="5929E7CDC9489E8E" modified="2014-04-16" version="1">MTTASTSTQYPASNPSCRLLPGDSSQVQPKAPLLHILHMAGAQEDTFTLKEVMHYLGQYIMVKQLYDKQRQHLVHCQDDPLGELLEVESFSVKNPSPVYEMLKKNLIVLNCTGNADAAKTLSVAKDSNHVTSSEDPGQTSSHPCKLSQALAAEGMSTSTTQPTSHRRQRETDGDSLDDLPRSACKRPKLDVSLEEWDLSGLPWWFLGNLRSNYGRKSNGSTDIHTNQDEDTAIVSDTTDDLWFLNEAESEQVSVEMKEAALERGSDGEEEEEKKEWDKEDKEMQDDLDEDSQCLSDDTDTEISTQDAWQCTECRKYNSPMQRYCVRCWALQKDWYKDCPRLVHSISVPDIPTSMGAQEDGEDDGIDIPDCPRTVSDPVILPSHSLERQRPTDKGKGRLPRPTGISEGLESSEGDSQDMLDMAQTMGDKQEAILEPCKLCRIRPRNGNIIHGRTGHLIACFSCARKLHKFHAPCPGCGQVIQNVIKTFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2K8</accession>
    <name>W5N2K8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibroblast growth factor receptor-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Has a negative effect on cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005465">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2K8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014867"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014896.1">
        <property type="protein sequence ID" value="ENSLOCP00000014867.1"/>
        <property type="gene ID" value="ENSLOCG00000012085.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156736"/>
    <dbReference type="HOGENOM" id="CLU_038830_1_0_1"/>
    <dbReference type="InParanoid" id="W5N2K8"/>
    <dbReference type="OMA" id="DPTGQPW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012085">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000246">
        <property type="entry name" value="Fibroblast growth factor receptor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000593">
        <property type="entry name" value="Fibroblast growth factor receptor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052615">
        <property type="entry name" value="FGFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890:SF10">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867157" description="Fibroblast growth factor receptor-like 1" evidence="14">
        <location>
            <begin position="24"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="149"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="262"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="377" mass="41072" checksum="AD821E3B7780D8E4" modified="2014-04-16" version="1">MFHNAESLTACLAALVLVLSCEAKGPPRVSRPVEERVTARLGRTVRLPCPVEGDPPPLVLWVKDGRNVHQGWSRHRLARQGLRIKEVETGDAGAYACRAANGFGSITINYTLVVVNDSAAGQNRPPSAPTEPSTERVGQDPTGQPWVKPRFTQATKMRRRVLEQPVGSSVRLTCLASGSPAPAVTWWKDEQRLAPPRGGARRQWTLTLRNLQPQDSARYTCLVANSAGHINATYKVDVIVLRNSFRTLTLGTHPVNTTVEFGGTASFQCKVHSDVKPVIQWLKRVEAGAEGRSNATLEVGGQHFAVLPTGEVWSRPDGSYLNKLAIAKARDEDGGMYICLGANTMGYSFRSAYLTVLPVSEPCFLSLRSTATPSPPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N350</accession>
    <name>W5N350_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01017292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630836.1">
        <property type="nucleotide sequence ID" value="XM_006630773.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203059.1">
        <property type="nucleotide sequence ID" value="XM_015347573.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203060.1">
        <property type="nucleotide sequence ID" value="XM_015347574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N350"/>
    <dbReference type="FunCoup" id="W5N350">
        <property type="interactions" value="1442"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015059"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015088.1">
        <property type="protein sequence ID" value="ENSLOCP00000015059.1"/>
        <property type="gene ID" value="ENSLOCG00000012241.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693011"/>
    <dbReference type="KEGG" id="loc:102693011"/>
    <dbReference type="CTD" id="64429"/>
    <dbReference type="eggNOG" id="KOG1314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155642"/>
    <dbReference type="HOGENOM" id="CLU_044394_1_0_1"/>
    <dbReference type="InParanoid" id="W5N350"/>
    <dbReference type="OMA" id="GCIHAAI"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012241">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="98"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="318"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="413" mass="47757" checksum="78FBD37372F7B52F" modified="2014-04-16" version="1">MSILSSFIKFENLHELKRLCHWGPIIALTVIAVCSTMAVLDSIIWFWPLDSTGGSINFIMLINWTVLILYNYFNAMFVGPGYIPLGWKPENPQDMCYLQFCRVCQGYKAPRSHHCRKCNRCVMKMDHHCPWINNCCGHLNHAYFTSFLLLAPLGCIHATVIFVMTMYTQLYDRISFGWSSVRIDMSAVKRDHRPIIPFSVSAFAATLFAFGLALGTTIAVGMLFVIQMKVILRNKTSIEAWIEEKAKDRIQYYQTGEEFIFPYDLGSKWENFKQVFTWSGTPEGDGVEWPVHEKCHQHTLTIEQLKQKADKRVRSVQYRVIEDYNGVCCPLSKGVKTFFTTPCTEEPRIKLTKGEMIFATRSIKWWMYGDKVLGEEQTKAGVRIRGWFPRRCVEKCLYDTANDAAMNEEKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N364</accession>
    <name>W5N364_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01017266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N364"/>
    <dbReference type="FunCoup" id="W5N364">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015073"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015102.1">
        <property type="protein sequence ID" value="ENSLOCP00000015073.1"/>
        <property type="gene ID" value="ENSLOCG00000012238.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156471"/>
    <dbReference type="InParanoid" id="W5N364"/>
    <dbReference type="OMA" id="REVYDCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012238">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:retina development in camera-type eye"/>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
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        <property type="entry name" value="PDIESTERASE1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="5 14 15" id="KW-0479">Metal-binding</keyword>
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        <location>
            <begin position="484"/>
            <end position="819"/>
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            <begin position="828"/>
            <end position="862"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="838"/>
            <end position="848"/>
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        <location>
            <begin position="849"/>
            <end position="862"/>
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    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="560"/>
        </location>
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        <location>
            <begin position="560"/>
            <end position="564"/>
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            <name>AMP</name>
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        <location>
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        <ligand>
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            <label>1</label>
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    </evidence>
    <sequence length="862" mass="99618" checksum="5F68384CEF0A13CA" modified="2014-04-16" version="1">MSVTQEDVEKFLDGNPDFTKHYFDKKLKPGAISAILGQTQVQVDFTSFKELSQVEESEILFELIRDMQESINMEKVVFKTLRRISFLIHADRCSLFMYRQRNGIAELATRLFNVNKDAVMEDCLVQPDSEIVFPLDIGVVGNVAQSKKTINVKDVSQCSQYSPFVDDLTDYKTRNILATPIMNGKDVVAVIMAVNKIDGPYFTEEDEDLFLKYLNFASLNLKIYHLNYLHNCETRRGQVLLWSANKVFEELTDIERQFHKALYTVRAYLNCDRYSVGLLDMTKEKEFFDVWPVLMGEVPPYSGPVTPDGREIIFYKVIDYILHGKEEIKVIPCLNPPADHWALVSGLPTYVAESGFICNIMNATSEDMFKFQAGPLDESGWTIKNVLSLPIVNKKEEIVGVATFYNRKDGKPFDEQDETITESLTQFLGWSVLNTDTYDKMNKLENRKDIAQDMVLYHVKCRNDEIQNILKTREVYGKEPADCDEEELSYILKKELPDPRKFEIYEFRFSDFDCTELELVKCGIKMYYEVGVVKKFQIPQEVLVRFMYSVSKGYRKITYHNWRHGFNVGQTMFTLLTTGKLKRYYTDLEVMAMITAAFLHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKFLLSEESLNIYQNLNRRQIEHVIHLMDIAIIATDLALYFNNRKRTMFQKIVDLSKTYENEKEWVDFLSLETTRKEIVMAMMMTACDLSAITKPWEVQSKVALSVAAEFWEQGDLERTVLQQNPIPMMDRNKAAELPKLQCGFIDFVCSFVYKEFSRFHDAITPMYDGLMNNRKEWKARADEYEAKMKAIEEEKKRKEEEAAAAAAKKAASANPSGGSQSKTCSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N388</accession>
    <name>W5N388_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Golgi SNAP receptor complex member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">27 kDa Golgi SNARE protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Membrin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 17">Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 17">Belongs to the GOSR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638192.1">
        <property type="nucleotide sequence ID" value="XM_006638129.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N388"/>
    <dbReference type="FunCoup" id="W5N388">
        <property type="interactions" value="1545"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015097"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015126.1">
        <property type="protein sequence ID" value="ENSLOCP00000015097.1"/>
        <property type="gene ID" value="ENSLOCG00000012271.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695746"/>
    <dbReference type="KEGG" id="loc:102695746"/>
    <dbReference type="CTD" id="9570"/>
    <dbReference type="eggNOG" id="KOG3251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183192"/>
    <dbReference type="HOGENOM" id="CLU_083740_0_1_1"/>
    <dbReference type="InParanoid" id="W5N388"/>
    <dbReference type="OMA" id="LKYDSRH"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012271">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:SNAP receptor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Golgi SNAP receptor complex member 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="24514" checksum="D702A13E7C71E443" modified="2014-04-16" version="1">MESLYHQTNKQIQEVQSSMGRLETVDRQSVHLVENELQARIDQIFSSLERLEILAGKETPSRRQNAKLRVDQLKYDVQHLQSALRNFQHRRYTREAQEREREELLTRSFTTNDADTSIPIDETLQFSSSLNSAHRGMDELLNSGSSILDNLRNQRGTLKGTHKKILDVVNMLGLSNTVMRLIEKRAFQDKFIMMGGMLVTCVVMFLVVKYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5F7</accession>
    <name>W5N5F7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5F7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015866"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015896.1">
        <property type="protein sequence ID" value="ENSLOCP00000015866.1"/>
        <property type="gene ID" value="ENSLOCG00000012889.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064550"/>
    <dbReference type="HOGENOM" id="CLU_175675_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5F7"/>
    <dbReference type="OMA" id="NWAIFDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012889">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043373">
        <property type="entry name" value="IGluR_D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36687:SF1">
        <property type="entry name" value="GLUTAMATE RECEPTOR IONOTROPIC, DELTA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36687">
        <property type="entry name" value="GLUTAMATE RECEPTOR IONOTROPIC, DELTA-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868825" evidence="1">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="80" mass="8996" checksum="63D38CDEF38498D6" modified="2014-04-16" version="1">MFILIVSWSLTWDIVVSDSIIHIGAIFDESAKKDDEVFRLAVADLNLNNEILETEKITISVEFVDGNNPFQAVQEGRVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7J1</accession>
    <name>W5N7J1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox even-skipped homolog protein 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638420.1">
        <property type="nucleotide sequence ID" value="XM_006638357.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7J1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016630.1">
        <property type="protein sequence ID" value="ENSLOCP00000016600.1"/>
        <property type="gene ID" value="ENSLOCG00000013460.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691634"/>
    <dbReference type="KEGG" id="loc:102691634"/>
    <dbReference type="CTD" id="30335"/>
    <dbReference type="eggNOG" id="KOG0844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161025"/>
    <dbReference type="HOGENOM" id="CLU_096516_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7J1"/>
    <dbReference type="OMA" id="TFSGMIH"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013460">
        <property type="expression patterns" value="Expressed in ovary"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000256">
        <property type="entry name" value="Even-skipped homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF3">
        <property type="entry name" value="EVEN-SKIPPED-LIKE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="44"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="252" mass="27961" checksum="7DADE8F276A2763C" modified="2014-04-16" version="1">MKPEVHTNTLPEDKESPVSAKGQGETDQANEEASPRNPFPGAVDQSRRHRTAFTREQLSRLEQEYCKETYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRHTLTWPYPLDPSLYTYMMSQAATGLPCPFLPHVPLHLYSHLGLGSGAVLPSPFGGALRPLDPLRLSHAPYPRHEILPGLPQPSIYPPSQGLHHPAGCPCPLCVCHRGPDHLRTPSARGDPLLGLNSSLGFKSAAAPLDGREEVSVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7W5</accession>
    <name>W5N7W5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01015153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5N7W5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016724"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016754.1">
        <property type="protein sequence ID" value="ENSLOCP00000016724.1"/>
        <property type="gene ID" value="ENSLOCG00000013564.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684400"/>
    <dbReference type="KEGG" id="loc:102684400"/>
    <dbReference type="eggNOG" id="KOG1559">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043388"/>
    <dbReference type="HOGENOM" id="CLU_058704_1_1_1"/>
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    <dbReference type="OMA" id="CHEDIDH"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013564">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034722">
        <property type="term" value="F:gamma-glutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046900">
        <property type="term" value="P:tetrahydrofolylpolyglutamate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000024">
        <property type="entry name" value="Folate gamma-glutamyl hydrolase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF10">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867398" description="folate gamma-glutamyl hydrolase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 7">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011083"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615527-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="35968" checksum="53D7AD04E8CEB42D" modified="2014-04-16" version="1">MCSSAIVLSVMVGVICYLPIPAFSSLSPNYRPIIGIVAQENLPSDPHAQGSSYIAASYVKYLEAAGARVVPLRINRTKEEYTKLFYSINGLLLPGGDVDLENSQFGRVSRIFYDLAIKANDASDYFPIWGTCQGFQEMTVLTSGKNLLTLTDTKAIALPLTFTSVGPNSRLFKSFPKDLLHALTTENITSNFHKWSLTLQNFTRNMKLKRFYKILSTSTDGHKEFISTMEAHRYPFYAVQWHPEKSQFEWIEKSGMVHTTAAVDAAYYTARFFVNEARRNHHRFANKIEEEKSLIYNYSPVFTSLESIFVQNYYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8N7</accession>
    <name>W5N8N7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dynein heavy chain domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8N7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016996"/>
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        <property type="protein sequence ID" value="ENSLOCP00000016996.1"/>
        <property type="gene ID" value="ENSLOCG00000013783.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107079310"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_900037_0_0_1"/>
    <dbReference type="InParanoid" id="W5N8N7"/>
    <dbReference type="OMA" id="MNTILFM"/>
    <dbReference type="OrthoDB" id="5986589at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013783">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
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        <property type="entry name" value="DHC"/>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_C_barrel"/>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
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    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="1">
        <location>
            <begin position="133"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="34501" checksum="B5B8AD7EFECE1F87" modified="2014-04-16" version="1">MLQDLEQRVQQLASSSVILGLSAGLVRELITDRSQILNSLLESQMSKLYVSDQPAAPDISQARERLQALRESMDRNEESRGARVHRPLQQFVQKEWDSIISVVSSMLSELSQSSGCNKTLSSWDSSQTLFLLHQLESQAKLLGAYLWEETPPAVYHLSAFQNPKGFLVAVLQEQARAEQRDISNFSLQYQVLKTSAIPSSPPHSGIYITGLELHGALWDTRLGAVQETLSIQPCQLPVVWVRATADITTATSSPSSFPLYLCPVYKDLIPGDLMDTDKITYIPLVAKLPPTLCKIRRVRIIIRQLTTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8Z8</accession>
    <name>W5N8Z8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal protein L4</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8Z8"/>
    <dbReference type="FunCoup" id="W5N8Z8">
        <property type="interactions" value="1382"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017107"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017137.1">
        <property type="protein sequence ID" value="ENSLOCP00000017107.1"/>
        <property type="gene ID" value="ENSLOCG00000013867.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018145"/>
    <dbReference type="InParanoid" id="W5N8Z8"/>
    <dbReference type="OMA" id="ALYGTWR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013867">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="5">
        <location>
            <begin position="276"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="389" mass="43879" checksum="8271B7A4471DB385" modified="2014-04-16" version="1">MACARPLISVYSEKGEASGKNVVMPAIFKAPIRPDIVNFVHTNMRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRINVTQKRYAICSALAASALPALVMSKGHRIEEIPEVPLVVDDKVESYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRKGPCIVYNEDNGVTKAFRNIPGITLQNVNRLNLLRLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKVDYNLPMHKMTNTDLSRILKSPEIQKALRPPSKKIHRRVLKKNPLKNLRVMVKLNPYAQTAKRHAILRRDPTIKAKMLKPKKAKKTAKTEKTKPAANTHAAMGGIPPFPLTPLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9D7</accession>
    <name>W5N9D7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor ISY1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Interacts with CPSF3; this interaction is in an RNA independent manner. Interacts with the microprocessor complex subunits DGCR8 and DROSHA; this interaction is in an RNA dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203518.1">
        <property type="nucleotide sequence ID" value="XM_015348032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9D7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017277.1">
        <property type="protein sequence ID" value="ENSLOCP00000017246.1"/>
        <property type="gene ID" value="ENSLOCG00000013988.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690876"/>
    <dbReference type="KEGG" id="loc:102690876"/>
    <dbReference type="CTD" id="57461"/>
    <dbReference type="GeneTree" id="ENSGT00390000014109"/>
    <dbReference type="HOGENOM" id="CLU_043453_0_0_1"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013988">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="285" mass="32651" checksum="65BFFCB23BB10821" modified="2014-04-16" version="1">MARNAEKAMTALARFRQAQMEEGRVKERRPFLASECNELPKAEKWRRQIISEISKKVAQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYGRVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPVPPPRKTRAELMKDVDAEYYGYRDEDDGVLVPLEQEYEKQVITEAAEKWKAEREARLAGGGAGEKEEEEEEYIYAVQDEEHSDEEMGEQLEGEEGSQTFIAHVPVPSQKEIEEALVRRKKMELLQKYASEALMAQSEEARTLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9N9</accession>
    <name>W5N9N9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/arginine-rich splicing factor 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Delayed-early protein HRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Pre-mRNA-splicing factor SRP40</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Splicing factor, arginine/serine-rich 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632776.1">
        <property type="nucleotide sequence ID" value="XM_006632713.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9N9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017348"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017379.1">
        <property type="protein sequence ID" value="ENSLOCP00000017348.1"/>
        <property type="gene ID" value="ENSLOCG00000014075.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694417"/>
    <dbReference type="KEGG" id="loc:102694417"/>
    <dbReference type="CTD" id="335396"/>
    <dbReference type="eggNOG" id="KOG0106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164884"/>
    <dbReference type="HOGENOM" id="CLU_012062_34_2_1"/>
    <dbReference type="InParanoid" id="W5N9N9"/>
    <dbReference type="OMA" id="MRRGPPD"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014075">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12337">
        <property type="entry name" value="RRM1_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12600">
        <property type="entry name" value="RRM2_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000028">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000138">
        <property type="entry name" value="Serine/arginine-rich splicing factor 5 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047190">
        <property type="entry name" value="RRM2_SRSF4/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF63">
        <property type="entry name" value="SERINE AND ARGININE-RICH-SPLICING FACTOR 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 15" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="114"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="73"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="178"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="188"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="301" mass="34586" checksum="9761C5B88C2063A0" modified="2014-04-16" version="1">MSGCRVFIGRLSPHARERDVEKFFKGYGRIREISLKNGFGFVEFEDHRDGDDAVYELNGKELCSERVTIEHARSRRGRGGGGAGGGGRFSPRFGSYRQSRSSGSRYGPPVRTEHRIIVENLSSRISWQDLKDLMRKVGEVTFVDAHRTNRNEGVVEFASRSDMKNAIDKLDGTELNGRKLRLTEDRKRNRSRSRSRSRSYTRSRSRSHSRSRSRSRSRSRSRNRSRTRTRSRSLSRTPEKKTTGRSGPRSPSRSRSRSRSRSASHSPPPPKRQSRSRSRSHSRSRSRSRSRSRSQSAETQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAV0</accession>
    <name>W5NAV0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Synemin, intermediate filament protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005026">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAV0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017759"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017791.1">
        <property type="protein sequence ID" value="ENSLOCP00000017759.1"/>
        <property type="gene ID" value="ENSLOCG00000014429.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTUH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159268"/>
    <dbReference type="HOGENOM" id="CLU_002793_0_0_1"/>
    <dbReference type="InParanoid" id="W5NAV0"/>
    <dbReference type="OMA" id="KMLYASE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014429">
        <property type="expression patterns" value="Expressed in muscle tissue and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043034">
        <property type="term" value="C:costamere"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060053">
        <property type="term" value="C:neurofilament cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019215">
        <property type="term" value="F:intermediate filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017166">
        <property type="term" value="F:vinculin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031443">
        <property type="term" value="P:fast-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030634">
        <property type="entry name" value="SYNM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47136">
        <property type="entry name" value="SYNEMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47136:SF1">
        <property type="entry name" value="SYNEMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="11"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="454"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="988"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="189"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1084" mass="122270" checksum="800527A248C0586F" modified="2014-04-16" version="1">MLQHRRPFENEKLQLQELNRRLGQYLSRVQQLERENARLATETDALRRDRTAEWERQYAAELRALRGAVDQLALERSSAALERDRLWRECQLLTGLCSQEAGLCREIDGELRGCEKQLQVAQKKNAALEQHLLQLQDEHASLEDAHRRDLALLREQVHARAAPALATHRHQGAPALAVEDVEEYAHSLSEAWEETLEVYRRRIEELEESARLGERRLGELRREQGQCASQLEQLRAEADREARRHERLERELGHVQDACSSALEQYQVAIEQLEEERRELASIIAEKLRDHQELMQVKMGLSLEVAAYRALLEGERRDVYQWTDEYVREVPRPSDIRRAAHTYAVKQSSVTRHDGRKLYPVSMLLDTRHKEQASTKNTVSRPARFTSASIAKTVPISISDRSSEARRDIPSFTKASQASVKLFKANDSETERKSTDVQKKTVVTEEVVKHSVTDSAVNLRSSGSVNRASPGPLPTAEKEPAPENVHLKTTNITSSTKVTQPEEQSPTETKEETADTKVHLIKMEQTPKSREVKITLEGPVRKDKKPLTTEEKVLDSITMEDIIEKVVKPAGLETVLSSSPDSKITYHVEKTERGDGTTKTEIILESKVQEDLDTSDDSILEELLNKGVKKVSMEDVKGTPTGSMIENLLSLGMKGSENLENKAVNVEIIEEPLEPHSDEENEGKPPPTFFKPTSMFFQIEELENAPQVARSSQSSTEAVGFSVTDGEYGKTMPFQEVYGAQGSSYIHVPVTEYFVSTPDENVSEHEESDFPIYEHYGMAQDLSGDESYWQEEPATAQEQSFTEADHYRTTYLNTNRGDHTMTRESFPECIVEEEVEEVQVPHDVQESVLKLLKEDDMDPKKQLKGALEQLQGTVTESFKEELAILTRNGKEGSDSVAVDIRKVEQSTEDGSVTIVAEINVSQSLEDSGLLEDEIKELSEEQVMSALQSANPELHQAISRGGEKSPAGGDNREFRVKVSRDQGVRMQGVSWVESSENPENTGDEEESQSGVSKTVKHIRLGPTEKSFTFQMDVTKMTTWDPSHGYTAEHAAFTRIIQTQRVVDPRAVVSEEKRIAAIYLDSPEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB49</accession>
    <name>W5NB49_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme Q8B</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB49"/>
    <dbReference type="FunCoup" id="W5NB49">
        <property type="interactions" value="717"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017858"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017890.1">
        <property type="protein sequence ID" value="ENSLOCP00000017858.1"/>
        <property type="gene ID" value="ENSLOCG00000014507.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158965"/>
    <dbReference type="InParanoid" id="W5NB49"/>
    <dbReference type="OMA" id="CRGYPES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014507">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032836">
        <property type="term" value="P:glomerular basement membrane development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF4">
        <property type="entry name" value="ATYPICAL KINASE COQ8B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="7">
        <location>
            <begin position="268"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="68371" checksum="C00B2B3766AC97A4" modified="2014-04-16" version="1">TMWGEAKQVLCGVGRVGAAFAETQAEQLWLLASNSSLTAGLKRVQNCAEDGLRSLLAARGDQTGRVDFPEASGWGEEEELAKWAVGSEMAPEVEGPEPQSSPLQRPASQHLSQQAAGLGGPGQTRHLHQDSALGRLTAEDIRKAREAKRPIERGGSSKPARQKLSDRAKERSVPATRFSRLANFGVGLAVALGLGAIAEVTKQSLGGKQTQANGRALLDSPFLSESNAERIVDTLCKVRGAALKIGQMLSIQDSSFINPQLQKIFERVRQSADFMPAWQMTKVLQEELGADWRDRLASFEEKPFAAASIGQVHLGVLKDGREVAMKIQYPGIAQSIRSDVNNLLSVLKMSVALPQGLFAESSIEVLQRELEWECDYRREAKCAKRFRELLDGDPFFTVPAVVDDLSTERVLCMELVSGVPLDRCIDLEQETRNQICSNILHLCLRELFEFRFMQTDPNWSNFFYDAEQNKVSLLDFGACRDYSQEFTDDYIEVVHAASVGDRQRVLQKSKDLKFLTGFETKAMQEAHVDAVMILGEAFASPEPFDFSTQSTTQRIQSLVPVMLRHRLTPPPEETYSLHRKMAGSFLMCSKLGAVIPCWEMFRSIYSAYAAGRRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC57</accession>
    <name>W5NC57_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BCL2 interacting protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC57"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018248.1">
        <property type="protein sequence ID" value="ENSLOCP00000018216.1"/>
        <property type="gene ID" value="ENSLOCG00000014789.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_039135_1_0_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Bcl2-/adenovirus-E1B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12496">
        <property type="entry name" value="BNIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="440" mass="49647" checksum="AB51B0E72A9BE54C" modified="2014-04-16" version="1">MAAEVTETEAGATGDTGFNASSSGADTPRLVEKVENRPAAPAPAPGEDAPQSGSAPPSETAEGAAPRTPTKTGGPTQEAGTPDNGRKQEPAGVRTSTPVSHSLPSPTAHNGSLLERQESVATTEARLRMEGVELKEEWQDEDFPRPLPEEEEDMQMDEELFTGREGNSATQSYELNSDKKAKKKLMAPYISLTLDRTEGSVLSDEMEESGELDLDDIDTPSDNSNEFEWEVNLPESQRQGINACTEIFISKQQSSVITKVVFRIFFLSNQVIYFSNHNVNKLLNFSYIHYMAEDIQEVFRITWDHLVKVNLICSMKWLHTVMSVEWWQGEQEKLNWNSKCIIQVECTVWKFLSFQVLVIKCWVRHTDMPESILSFHSSKFSQKIKYVFSLVELAELVPMEYVSIPECIKQYEEEKNRKRRKRIDRDMQGKQEPAAPATEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCK9</accession>
    <name>W5NCK9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCK9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018400.1">
        <property type="protein sequence ID" value="ENSLOCP00000018368.1"/>
        <property type="gene ID" value="ENSLOCG00000014919.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_275559_0_0_1"/>
    <dbReference type="InParanoid" id="W5NCK9"/>
    <dbReference type="OMA" id="CAESHRE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014919">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="887"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="927"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1010"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1158" mass="119360" checksum="C7DF3813C25BB8D0" modified="2014-04-16" version="1">MANEKENPGSTGYRLQRRTPSLLKRQSLPAPGAQARLALVRRPLRDTWNLQEAPGSARGGPKEPGSECGDGLGVPGKRTSVPERRFSLDSANARPCREPVAMAAKGGKAPLKELTNRAREPGGEGAGRAAKEPSLEATFEIAPAADDPRPAFFPALLGADPDRSLPVGLLQVEEDGAGAAGGGALSPVSEHLRQVHEKLSRRRYFREPSAGQPARRCLTPVLEELALPEEEKAAPLDRICSLYRSFFAVECEGLVEQILSNPSSPAGRGGTPLLGGASAGGWLSIELEPPPQGPGLADSVVEPPCGGLEASEMSEVSLLNLDELDMELVKLKQAGGAAAAAAGGSEEPCSSPRRRSPVALLMERIQSLSSNAGSSLEGTFNVSDRKEGGQAAEADGQEGSRGCGEAGSPGAKANVTLILCPPSAAEPKCGAVDPPELAAAPGQGAVNENDTWELTLGGGEGKPATSDSKDRMSVGNSFSSDVCPLLGDLDSILSTDDSLVRSHPLISSTPLAVPDVFNFHQRATSNPAPSQREAVKANSSAGSEEGSVPPLTDRLLNSTRASCAAASGFPKPKPGTSTRRASLGGQPSGVAKNRKSLLPPPPPPPPPPPAKTGLQANCFSHPRMLDLPKPGPSRRLSLTYPSGPVGAAGSCPPSAVLRKPPRSAGKQLDGIPKPAARGQTPASSAGESSAGAPSSVLSRLSFGGRGEHLSTAGNRLQASGLPKPAASGLRAPVSRRSALGPKVPGTQPGRGTAASSTANPKELLGAPGSEVPGSSSRVRPQTPKLARKTADETLLPVAKRKKVDPVSSGIAVPKVPGSAGAPRGLRRPATRLSGRIPESRETGPPSSKGLQAGSALLTAPKLGLRPPSVCLSKRSKGAVPSADHGSATAQPQESEVSALSANVTFGAKTPPAATPEIANKGAAQSRLDPKDPSPERAAQDESSANVTFETARPAGPRTQNSVPSPPVNRTLDIASPGRAGSLQEEPAGGAAASWAGPPPQSDSPAAPLQNDDSSPSANLTFEAGERAGSPVEENLGVSSLDEQAASPPDKSQGSASCSGCAESHRESRALLARCRRLEEQLKAGSAQCELYCQENEMLQERCRLLEEQLKAGQLFLLRAKANSRLFCACTRTKSLILQRVPQKQTPTFSGEVELHIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCP2</accession>
    <name>W5NCP2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PDZ and LIM domain protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9. Interacts (via LIM zinc-binding domain) with MKRN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01006221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCP2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018401"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018433.1">
        <property type="protein sequence ID" value="ENSLOCP00000018401.1"/>
        <property type="gene ID" value="ENSLOCG00000014946.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160418"/>
    <dbReference type="HOGENOM" id="CLU_038114_1_1_1"/>
    <dbReference type="InParanoid" id="W5NCP2"/>
    <dbReference type="OMA" id="MRGHFWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014946">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09449">
        <property type="entry name" value="LIM_Mystique"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000085">
        <property type="entry name" value="PDZ and LIM domain 2 (mystique)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000130">
        <property type="entry name" value="PDZ and LIM domain 2 (mystique)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF1">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="14">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="288"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="361" mass="40080" checksum="1CD4A737F203FDBD" modified="2014-04-16" version="1">EMSLTVNLIGPSPWGFRISGGRDFKKAIAVSKVNGGSKAESAGLRLGDVIAEINGESAAEMLNVEAQNKIKSSKTKLQLVVDRSEPLSPGQTNGLTSPEHLAVRFQEALQPSRDENYNDYSFSGPPSLSPTPYSAERKRDAHTTFSTKSFQTHSWSPVEKVASPRTSSRPVSQEFVTDVRSNSLSRTPTPPGRYSPQSPREREPPLSPRRSSDAGMQRFDRDSEVYKMIQENKESRSAPRQSSTFRLLQEVLEADEKGLPEAALRFPGKLSPNAPKPSSSVAGVQKFHTCEKCGTSIVTQAVRIMEDRYRHPECYTCTQCCLNLKMRGHFWVGDEMFCEKHAKERYQGPGSTHVPMVSPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDW7</accession>
    <name>W5NDW7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cleavage stimulation factor, 3' pre-RNA, subunit 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDW7"/>
    <dbReference type="FunCoup" id="W5NDW7">
        <property type="interactions" value="729"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018826"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018858.1">
        <property type="protein sequence ID" value="ENSLOCP00000018826.1"/>
        <property type="gene ID" value="ENSLOCG00000015300.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158987"/>
    <dbReference type="InParanoid" id="W5NDW7"/>
    <dbReference type="OMA" id="HSMNTNA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015300">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12671">
        <property type="entry name" value="RRM_CSTF2_CSTF2T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.630:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000061">
        <property type="entry name" value="cleavage stimulation factor subunit 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="50"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="589"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="663" mass="70664" checksum="2880920CF825CD26" modified="2014-04-16" version="1">MSLWVPRHGLERRGSNVSCRKSRFLSKMASTVVAAAAAAGRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGLVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGDPCQPEEAPESISRAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKMLHRQAALQPLIPSSQAAQGPLSNQALVQSSVPVSQPQPMAGMHVNGAPQMMSAPQMVSGVAGQVPGQGPVVPAVGMQPQIGIPPGGPVPMDRGQVPMTDPRAPMRGGVPPVSAVVAPRGLLGDGPNDPRGGTLLSVTGEVVEPSRGFMGAPSHQGPPMHMTHDGRGPPPDMRGPHDMRGGPMGEPRGMMGDPRGPMMDQRAPPMEARGSHDSVYHEKDLLLEMLFCRMQAFRNPVLCLAPIAGGRDPRAVEARSLEARGPVPAQRVPMATGIQGPVPHTMGANVPQPQRQVHPSSSCCSCPPPHRGTGGPPKNMSGTRLQSCGSHSMFSFAKRMFAPCARGQTAGLTSRQLKVHMGPWPGGHMAPGAGRGHQAPGVPGVPQTGGFSPAQNQVTSQDHEKAALIMQVLQLTPEQIAMLPPEQRQSILILKEQIQKTAGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF10</accession>
    <name>W5NF10_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nephrocystin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the nephrocystin-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF10"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019219"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019251.1">
        <property type="protein sequence ID" value="ENSLOCP00000019219.1"/>
        <property type="gene ID" value="ENSLOCG00000015611.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU7K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007701"/>
    <dbReference type="HOGENOM" id="CLU_024987_1_0_1"/>
    <dbReference type="InParanoid" id="W5NF10"/>
    <dbReference type="OMA" id="CYLIALM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015611">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090251">
        <property type="term" value="P:protein localization involved in establishment of planar polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11770">
        <property type="entry name" value="SH3_Nephrocystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000171">
        <property type="entry name" value="Nephrocystin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039687">
        <property type="entry name" value="NPHP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030642">
        <property type="entry name" value="NPHP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15176">
        <property type="entry name" value="NEPHROCYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15176:SF1">
        <property type="entry name" value="NEPHROCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="211"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="693" mass="78001" checksum="71CC13D0F5C96AD7" modified="2014-04-16" version="1">MPPKSKSPLQTVQRNTDEIKRKVDSLFQEIQQPDATQHPKQTEAFQSRCIKLQKSVEETLKTLRALNKADEPVPVGTYDQRKREEDMRLLNLQHQLQMLAERLVPDTAISARIEKASTSKVEDEEEEEDNEEESEEEDDEEESEEEDEVGPSVNNFIAVCDFNSQQEGDLTIKKGEILRVLSKNPDGWWLAQDSKGNKGVVPKTYLRVGVNCNDEEEETETEEDSEEETDEVKQRAKTDKQKEQRKHRVDRDGKRNDGTKSNWDAVRKAVTEISASDVLSAMGAIPAGFRPSTLSKLLEEGKAFRSSHFLQPEYSQSQLAFKDLLWSPDKGSVRPRVSRVSLTLTLWSCKMIPPPGVGVQVLSRHVRLCVFDGTRVLSNIHTVRASCNNKNTRTWTFSPRVSGILPSLLDGDCFVRSNSQSPELGILFELGISYIRNATGERGELSCGWAFLKLFEASGVPVPYRTYELAVNGGTPYDKGVDVDPSIARRASSSVFQQMMTSRRQPKFFVKLKYANSKTRTALQLLPDTLVGSTCSIHLLALYRHILADALLRDRLTMQNADLICSPVLATFAQVLDQTDLLDALRSVWAEKEGSLKRSEKRDGEFMKTLFVQLYMDTVYPLLHSLALPPCQWADQETESLRWRIIAGILAVNKQKESALSSLLSEEAAQEAFDVSEVAYDFLTKARETAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFK4</accession>
    <name>W5NFK4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho guanine nucleotide exchange factor (GEF) 33</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFK4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019413"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019445.1">
        <property type="protein sequence ID" value="ENSLOCP00000019413.1"/>
        <property type="gene ID" value="ENSLOCG00000015769.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163420"/>
    <dbReference type="HOGENOM" id="CLU_018772_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFK4"/>
    <dbReference type="OMA" id="LEHANMV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015769">
        <property type="expression patterns" value="Expressed in zone of skin and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042849">
        <property type="entry name" value="ARHGEF33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46944">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46944:SF1">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="179"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="748" mass="83201" checksum="DAAB01A2CAAA1F34" modified="2014-04-16" version="1">MNLQIAELQALAAELKSGLTGALQDISNIQQRDHNLEESMMSFHSEVDEKLLGMRNSLNTFKEELNTALSQIKDINSRQREMQKGLELFQMEVGRELLSNQPDCPHRKDQKEDITADGCQYEASQAGLSTIQHYFASLQSTSSSKVSPEQESGTHQRGLCKSPVWGDGAEALSIQDMGKRQNSALELLESERMYVSYLSLLLKANITFNGSEAVHVKDKRLFPSSLRFLIQQHLELLHGLQERLLRSQWQGIVGDVFMKLASKESDFLDYYVAYLKELPECLSAVNMYCASSFKAASLLEGDGNGDELRPSLHALLLQPVQRIPEYLLLLQNLWKHTEPEHPDYYLLLICIQQFKAFTSQCGQLLLHNEELLRHDRRDLKRLPTKHLFRTADCGVKSEELSSTISPSSALLQRASQVKRSKQKLLDQMQSKRAQDWEGVTRQYDSAKNVSQEFFFSPEVEPRLRSPALQSIPEMEQEHGSSERLPCKGPPPGDGVGEFLLPGEAPVLDSLYEDEDSLHDVSLLDNGSTASSNSSVDTAFARCSRGYAGEACGGGSRLPGRGCTSPEEPGPRRSRPPQAAQRKSKSLNGLQLDGPGGTPSHSPQGSAAGGPHAKLERQSSKGSAPRRAEAERRAAPEEEGLSRDRDKVPLPSPSHQEAGGQVWCEESPWRSRPEDHGQTAFSERSRKQDQKGGFRSSFKKLFKKKSSGTDIKEKANEKPISEPLVFSDQEILKTPRSARIGEIDRGTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFK9</accession>
    <name>W5NFK9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Condensin-2 complex subunit H2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-SMC condensin II complex subunit H2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND2 H2 (condensin-2 subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFK9"/>
    <dbReference type="FunCoup" id="W5NFK9">
        <property type="interactions" value="1300"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019418"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019450.1">
        <property type="protein sequence ID" value="ENSLOCP00000019418.1"/>
        <property type="gene ID" value="ENSLOCG00000015775.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014443"/>
    <dbReference type="InParanoid" id="W5NFK9"/>
    <dbReference type="OMA" id="FDPPEHK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015775">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051306">
        <property type="term" value="P:mitotic sister chromatid separation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031737">
        <property type="entry name" value="CNDH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009378">
        <property type="entry name" value="H2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031739">
        <property type="entry name" value="Ncaph2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14324">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14324:SF3">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16858">
        <property type="entry name" value="CNDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06278">
        <property type="entry name" value="CNDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin II complex subunit H2 N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin-2 complex subunit H2 C-terminal" evidence="10">
        <location>
            <begin position="481"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="70491" checksum="BDB69FC566634374" modified="2014-04-16" version="1">MINDSAKSRFNHLLQPIRDLSKNWEVNVAAELEEYLDELDQIPISFDDGATTMNFAEAALLIQGSACVYSKKVEYLYSLVYRALDFISNKKRDKQAPSVGRDGVDNDATFAYRNDDEELLSLDDIGDNSKTNCELRKEIGSKTLTIAPLTPLALSPPDEENKINLLRFCSNKCEILASYSDFRMHNSTPHGNGTFLLVAAGDTSQSLWNLQINHKDNSVVHEDAMDVNRMVLQEVSDHEALEPALNISGEGKDTARRNAGQAFDPQGDDNEMDVEVGPVHDIHIERHQISGDRRMLRERPPVPSENEKDKEILDPWKSLDPFKASEDKPLKKGRYYIIPQRVQEVEGNKRKRKVPNKLLDFLKWFSGVSSESSGRKIKKGPTFADLEELHCKQVKEKLAAQKMLKRKTGTIYFEKCNELQNKELVNAEEQNEAEEVRHDDYLDNDEGGDFSDHEDFAEDCPAQRAEDLEVVATAPEREGSSYEDLILKHVELFIAESQKYAQETALSLKIKEWENKMGPHLTAQEERLAFDIHDYGGKIISAFDSVGERRTFASIVKGKEEYEVCRYMLASLQLANDYTVEIHEKTGLEESLDSMALTLLSKRQAHERFKTYTAPSISETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGH5</accession>
    <name>W5NGH5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cadherin-like beta-sandwich-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGH5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019734"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019766.1">
        <property type="protein sequence ID" value="ENSLOCP00000019734.1"/>
        <property type="gene ID" value="ENSLOCG00000016022.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTA2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270620_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGH5"/>
    <dbReference type="OMA" id="AWKSKQA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016022">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025883">
        <property type="entry name" value="Cadherin-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12733">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cadherin-like beta-sandwich-like" evidence="3">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin-like beta-sandwich-like" evidence="3">
        <location>
            <begin position="109"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="968"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="859"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="971"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1202" mass="133083" checksum="93BC83A80DC17F15" modified="2014-04-16" version="1">MDNCDLEKLTVSAGMLHPTFSPLLTSYKVIVPSNISKITLDLLTSDSGASYKILCGSGSMVIELKDGVNKVDIEVIAEDGTAKIYTVEVTKLSASAAELSGLEFPEGLQLQPAFATTIFEYSCTVPFNLNALTVQPKVPDSNMKVSVNEADPSDSVPLTVGDSLLEVKVSSADGTRSCIYTILITREQIPYVITFNTVKDQMEYECPVSLTAFYRPVSISGSDPKHAFSAPYIDVLTRRSKVDPLDESPLGESWKVTEYEVDRRMSAASVKCCFAYRGCEIVLKLSELGEHVTHCPHKPPSELDVKDVTDSEWYKAQSASSDSLQIETKHTLQKKIRSWEKRLQKALMEDDVDKLCHHAEEHIKLYKQRLPKSGGITLNEEGESPLDALHQVLVDYASAIKLKPRDPNLHFQLGLAFEEQYYATEIYGLKRKVEDDPQDLSSAKASGREDEILAICKLHGLHGKPTLENQLKALDAEFHQLKEQGQSARADYIQTLFIWLSKKAGKVTSSVIYSSKRCSSDGRTGVSDEESPLHQAFLKHLDAWSLNPDNWELNLYVGRFLLLQGKCKEALQHLQTALILRPSWAPTRFYTGLALLQQEGGAGSRVQEAVMYLQQGLEQLLSQQCITEQYTQERKNGLDSANLFSTVNTQLLRGCLSLGTLLKTVKCPEKTMNPEQVFHITTFSWRLRFSVADLAARSVSRCVSRGAALQQLEWTLLEAHYSLVETLLAKPRGNESWIAKRCQGLTALIRLTAIPPCRELLDMQNKVCQLGVLATPCSSHALYLLGLAQLAQYDSDPDSEQGQRAISDARLSFQASINLENMPDSGPPPEKLKTGQKWWQDWRAREKEKEKTTQQSLTAAGAAGSSSPMPAGKGATRGKGVTARGSVPTPAKASVPSRGGGTPSPRAVPMSKTTAPGSRSHGKYLVNTSLMYSIKKHRSTYPVLCTPTTFYCSFSTRWQASVVTPEKLNGASKPATKTSSKSQLAPRRSDKQECCQPPQIKPVTAEETGEAVLLRTKVEESFLSVGNITQELVPASPANSPISINRGSHTHRLGLARALSRTEDTQDQACKLYEEVITMAPEVHDAYIELADLLIKTDPLAAVDVYCKFPLKPVNEQSFDDAFITGEIVRILMKQELYEHPNLASTFIAYGKVMGLGCLEKYINILEGKYKTKLLKTIYAGIHDKSVEDQDLQEFFRFKCWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHY6</accession>
    <name>W5NHY6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">H2.0-like homeobox protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Homeobox protein HLX1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the H2.0 homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008927">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626036.1">
        <property type="nucleotide sequence ID" value="XM_006625973.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHY6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020279.1">
        <property type="protein sequence ID" value="ENSLOCP00000020245.1"/>
        <property type="gene ID" value="ENSLOCG00000016392.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690652"/>
    <dbReference type="KEGG" id="loc:102690652"/>
    <dbReference type="CTD" id="327096"/>
    <dbReference type="eggNOG" id="KOG0488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183093"/>
    <dbReference type="HOGENOM" id="CLU_043671_1_0_1"/>
    <dbReference type="InParanoid" id="W5NHY6"/>
    <dbReference type="OMA" id="GQHQYQD"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016392">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000249">
        <property type="entry name" value="H2.0-like homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052497">
        <property type="entry name" value="H2.0_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46808">
        <property type="entry name" value="H2.0-LIKE HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46808:SF1">
        <property type="entry name" value="H2.0-LIKE HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="227"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="65"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="282"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="379" mass="41614" checksum="7BC999F3A09DACCC" modified="2014-04-16" version="1">MYTTGLNPFYPSNFSLWSAYCSSGFPLDSVKKPSFCIADILHVGDAENIPGSSAIVAHLGPHSHVHSSGSPLRPSPLAPESSVFSPRVSPASSFHRHHGIHGASHGSRLQLNCSPQQAPAPASKDLKFGIDRILSTEFDPKAKECTTLRDLTSIISSNRQSGLHVSLPSANHYFASLEPGISDPTSIMSSVGCGSRQSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSRAVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKEKEQPDKSPASVAQDAEQKQNESECESGGSDSEFEDGQDKSDNDIAEHNKTRVINPGAVSLHTEEASSSKAITETTALLSSQILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIB9</accession>
    <name>W5NIB9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="19">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17 19">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01001701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000020413.1">
        <property type="protein sequence ID" value="ENSLOCP00000020378.1"/>
        <property type="gene ID" value="ENSLOCG00000016483.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183075"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016483">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19679">
        <property type="entry name" value="UBR-box_UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2670:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047508">
        <property type="entry name" value="UBR-box_UBR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="21">
        <location>
            <begin position="99"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="18">
        <location>
            <begin position="99"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1270"/>
            <end position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1764" mass="200794" checksum="C46FE7A2DCB60C39" modified="2014-04-16" version="1">MAAAEADVDASPPPNGEFLDFSPRDTALKWRHASDLPKEIYWHLAQNVPKIYCLGPNLNPKSEDLWAQLILLAPLEWYLCDEDPTVGLSKLEQANKPSHLCGHVFKVGEPTYSCRECASDPTCVLCMECFLGSVHRDHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDWTEEDPLAHLSEDMIAHAYNIFSIMLKYAVDMLTWEKEEELPTGLELLEKADTYYCMLFNDEVHTYEQVIYTLQKAVNCSQKEAVSFATTVDRDGRKSVRYGDFQFCEQAKSVIVRNTSRQSKPLKVQVMHSSVVAHQCFGLKVLSWLGHIIGYSDGLRRILCRVGLQKGPDGENSSLVDKLMLCDSKLWKGARSVYHQLFMISLLMDLKYKKLFAIQFAKNYRRLQTDFMEDDHERVMSVTSMSVQLFTVPTMARMLIVEENLMATIIRTFIEHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPSEWSDQLREKFLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCARDEKVLIEAYKKCLISLMHCHSGFADGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHVLLSRTEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLMMLQAGASVMDPNHFLMIVLSRFELYHIFSTADYGKRYGRENTNKDMIQQNNTLIEEMLHLIMMVVGERFTQGIGQVQGCDEVKREIIHQLCIRPMAHSELVKALPEDENKETGMEKVIELVASFKKPGVTGRGLYELRPECAKEFNLYFYHYSRADQSKAEEAQRKLKRQNGENTALPPPALPPFCPLFASLVNLLQCDVFLSILSTVLQWAVEPSGGYWSESMLQRVLHLIGMALLEERQQLENSSEDNDVTFNFTLKISRPGEAPSNSPSVLAMLETLQNAAHLEVHKDMISWILRTYSNIKKIREQTTPSPMSETGGQTIEEMVRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFINENKELFQQSLEELEASTSAALEHSPTSPDTALVCVGPRRTRVSEKRQVVTCILCQEEQDIRADSRAMVLAAFVQRSTVMSKNRRRPFHDPETHDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLSRSCSSYSLLCFSFSSAEQPNLSQWLKTVSWQITALHSLRRKQDSSSSPGADDGERMDENQLSEGFRPDLIPMNPYSGSIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLADEEKPLFGSLPCRQDDCLRSLTRFGAACWTVSSLTVVQNHFLRLLAALVPDDQVENTPCILDIDMLHLLVSLVLSYPALHCQDSSGLSLDTAQLHIFHLVTVAHIVQILFTSVPAETSMDQENASGEEESAACFLYSVLRLHLGSALREDLSGWHLWHCVKAGLMPYLRGAAVFFHYLNGVPPPVELQGLVLGSSQFEQLCGYLCLPPNPFQLYHANRELYDPLLQGWCSHSSIQNYLQSSTPLISYPRESNRLIDLPDDYSSLINQASSFTCPKSGGDKSRAPTLCLVCGTMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCRERYRKIQKLWRQHSVTEEIGHAQEANQTLVGIDWQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIG3</accession>
    <name>W5NIG3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Lutropin-choriogonadotropic hormone receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LHCGR</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIG3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020422"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020457.1">
        <property type="protein sequence ID" value="ENSLOCP00000020422.1"/>
        <property type="gene ID" value="ENSLOCG00000016530.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157364"/>
    <dbReference type="HOGENOM" id="CLU_006130_1_1_1"/>
    <dbReference type="InParanoid" id="W5NIG3"/>
    <dbReference type="OMA" id="ASRCKLT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004964">
        <property type="term" value="F:luteinizing hormone receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042700">
        <property type="term" value="P:luteinizing hormone signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001541">
        <property type="term" value="P:ovarian follicle development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022602">
        <property type="term" value="P:ovulation cycle process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000019">
        <property type="entry name" value="Lutropin-choriogonadotropic hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000128">
        <property type="entry name" value="Lutropin-choriogonadotropic hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002273">
        <property type="entry name" value="LSH_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF1">
        <property type="entry name" value="LUTROPIN-CHORIOGONADOTROPIC HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01144">
        <property type="entry name" value="LSHRECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005150890" description="Lutropin-choriogonadotropic hormone receptor" evidence="14">
        <location>
            <begin position="20"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="394"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022266"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361222"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="690" mass="77964" checksum="05E6A6D525C4E0F5" modified="2014-04-16" version="1">MKLSLVCMIFPLLLSTRYSISLFNFVCPEICHCSDGIIRCNNETEWLFSASRKDKCRRITLTHLSIKTITSHSFEGLVNVLRIEISQSVFLERIEALAFNNLHNLSEISIQNTKSLVNIGRRAFSNLPKLRYLSICNTGITVFPDMSTIYSIESHFILEICENLHIESIPSNAFVGMTKEHITMNLYKNGFREIQRCAFNGTKIDKLVLKGNSKLRIIHEEAFIGGIGPDVLDVSSTPIQKLPTSGLESVQILIAQSTYALKSLPPLNLFSSLRKAHLTYPSHCCAFQNWKAKEHNSLFASWANLSSQHEEDRIMSQVSSLVTQIPATASVTSSEEDSEALSLEFHYPEFDFSQPLKILQCSPKPDAFNPCEDIIGYGFLRVLIWFINILAIVGNLIVLLVVMTSQYKLTVPRFLMCNLAFANFCMGVHLLMIATVDFKTRSQYSQYAIEWQTGAGCSAAGFFTIFGSELSVYTLTVITLERWHTITYALQLERRLCRRQAVLIMMGGWLFSLTMALLPLLGVSSYMKVSICLPMDIEILPSQAYIVIILLLNVIAFFIICACYIKIFLAVRNPKFTGKNRDTRIAKKMAVLIFTDFTCMAPISFFAISAAFKFPLITVTNSKILLVLFYPINSCANPFLYAIFTKAFRRDMCILMSWFGCCKKKANMYRMSFHYADHHTGKHSSASTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ21</accession>
    <name>W5NJ21_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Prospero homeobox 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_069198617.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5NJ21">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000020666.1">
        <property type="protein sequence ID" value="ENSLOCP00000020630.1"/>
        <property type="gene ID" value="ENSLOCG00000016698.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689446"/>
    <dbReference type="CTD" id="30679"/>
    <dbReference type="eggNOG" id="KOG3779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154790"/>
    <dbReference type="HOGENOM" id="CLU_016051_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJ21"/>
    <dbReference type="OMA" id="NGDNHNF"/>
    <dbReference type="OrthoDB" id="10038576at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016698">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021884">
        <property type="term" value="P:forebrain neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070365">
        <property type="term" value="P:hepatocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070309">
        <property type="term" value="P:lens fiber cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048885">
        <property type="term" value="P:neuromast deposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048886">
        <property type="term" value="P:neuromast hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.500:FF:000001">
        <property type="entry name" value="Prospero homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.500">
        <property type="entry name" value="Homeo-prospero domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023082">
        <property type="entry name" value="Homeo_prospero_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037131">
        <property type="entry name" value="Homeo_prospero_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039350">
        <property type="entry name" value="Prospero_homeodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198">
        <property type="entry name" value="HOMEOBOX PROTEIN PROSPERO/PROX-1/CEH-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198:SF10">
        <property type="entry name" value="PROSPERO HOMEOBOX 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05044">
        <property type="entry name" value="HPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51818">
        <property type="entry name" value="HOMEO_PROSPERO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Prospero" evidence="8">
        <location>
            <begin position="580"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="448"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="740" mass="83326" checksum="1610C001DB8D7DFF" modified="2014-04-16" version="1">MPDHDSTALLSRQTKRRRVDIGVKRTVGTASVVFAKARATLFSAMNPHGSEQDVECSVVQHADGEKSNVLRKLLKRANSYEDAMIPFPGATIISQLLKNNMTKNGGTEPSFQGSGLSSTGSEIHQEDACSNSSRESPQECLSPFSRPSMSQFDIERLNDEHLRAKRARVENIIRGMSHSPSVTLRAGENDREGAPQPPSPRESYRENKRKQKLPQQQQQSFQQLVSARKEQKHEERRQLKLQLEDMQKQLRQLQEKFYQIYDSTDSENDEDGNLSEDSMHSGSMDQRAQDSTADRSDNEMSDLDPGQFIDRARALIREQSMAAENEKPKRDSQRGKAQGPPSMHAEGKQLAETLKQELNTAMSQVVDTVVKVFSKPPRPVPQVFPPLQMPRDRFAVNGENPNFHTANQRLQCFGDVIIPSPLDTFGNMQVPSSNDQTEALPLVVRKNSADQTPSAPNPGGHHHHPSLHPSPLSATVGFSPPSFRHPFPLPLMGYPFQNPLGTPSGTYPGKERASPESLDLSRETTSLRTKMSSNHMNHRSCSPTHPGSTTEGLSLSLIKSECGDLQDMSEISPYSGSTIQEGLSPNHLKKAKLMFFYTRYPSSNMLKMFFSDVKFNRCITSQLIKWFSNFREFYYIQMEKFARQAINDGVTGSEELHVSRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIVAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKD4</accession>
    <name>W5NKD4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TBC1 domain family member 30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKD4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021093"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021129.1">
        <property type="protein sequence ID" value="ENSLOCP00000021093.1"/>
        <property type="gene ID" value="ENSLOCG00000017068.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159226"/>
    <dbReference type="HOGENOM" id="CLU_012937_3_0_1"/>
    <dbReference type="InParanoid" id="W5NKD4"/>
    <dbReference type="OMA" id="CKTNNLG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017068">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000011">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000009">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032738">
        <property type="entry name" value="Tbc1d30_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15733">
        <property type="entry name" value="DUF4682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="239"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00067508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="738" mass="82696" checksum="7BBB00E132D8227D" modified="2014-04-16" version="1">MASPVQLGRSELLEIDICAGRGSGGEDGGSPPADGFSAFQRWVPARRKTVRESSQRGENEGFPAEQNGTPATRPSPTQPRNGEIVDCLLVELYDTYSGSGGKSADSVDSSTEASSSDAFLGRSNTGSNFLLELQEKHTRRHQMKYLAQKDTEELKWIVQEVNYRIGIQSAKLVRQLRRKDRLYHKLQKNSDIVTACLQAVSQKRRVDTRLKFTLEPSLGKNGFQQWYDALKAVARLPTGIPKEWRKRVWLTLADQYLHSISIDWEKTLRFAFNDRSNPDDDSLGIQIVKDLHRTGCSSYCGQEAEQDRVVLKRVLLAYARWNKAVGYCQGFNVLAALILEVVEGNEGDALKVMIYLIDKVLPDSYFANNLRALLVDMAVFRDLLRLKLPELSQHLDNLQKAANREVGGSYEPPLTNVFTMQWFLTMFATCLPHHTVLKIWDSVFFEGSEVLLRAALAIWARLGERIESCQSADEFYSTMGCLTQEMLEHNLVDSNELMQTVYSMAAFPFPQLAELREKYTYNITPFPAHVKTSSGKLGRGRDSDEEIDIDDEDALVSAIGCLGPLSGLLGPELQSYQKQLKDHKGEQSLRSCGNIAELSPGAVDASRSDYQATFNSMMMERMTTDISALKKQYIRIKRRQQQQASLVYIKPDKGPATSLLPSQIPQSPVMNHLLLGKKLKPSERGCRNAIVHVPGSGSPPTPQEDLRAKLNSPWRTHVRVHRKNVARAKGQLGYGDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKH8</accession>
    <name>W5NKH8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 13 member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021137"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021173.1">
        <property type="protein sequence ID" value="ENSLOCP00000021137.1"/>
        <property type="gene ID" value="ENSLOCG00000017106.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="InParanoid" id="W5NKH8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017106">
        <property type="expression patterns" value="Expressed in embryo and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015370">
        <property type="term" value="F:solute:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF63">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="612" mass="68014" checksum="BEC34CEF1FACDA1F" modified="2014-04-16" version="1">XKLWAARKMLLVVFIPLSLLPLPLIHPSSEASCAYVLIVTAVYWVSEAVPLGAAALVPAFLYPLFGVLKSSEVNLPPFCVTETPGPRVSRGQPGTTPSTLIYARIDFGCILLAAQDQIKDILGEYQGRRCNLGSDSGESSTFLLHSPSLSDVDSSLVLISQSGSRCKSDQGDRTLQDSSESLGYLNRDLGIGSVLTSPFWHSDHDILMVEITGTGIKFGKGLPEYKENKWNMREKTPPEIKVSKQINDNRDMTIIINNIVIPHTALIWTLHYTCSPLIVPPLPPSRYPEAKVINFGTWFIFSLPISLVMLFLTWLWLHCLFLGCNFRETCSLSKKRKTRREELSERRIQEEYEKLGPVSYPELVTGLFFLLMTLLWFTREPGFAPGWTSLFEKKGYRTDATISVLLGFLLFLIPARNPCSSASGSAGKGGRPEGGADSDPMAPMITWKDFQKLMPWEIVVTAVQLYKEKATRRVQTGGERGRELNSLSLSRREADTPPAQLLPRHRAPAVYRIPLLFLSCRSETLRINPLHTLIPATMCVSFGVMLPVGNPPNAIVFSYGHCQIKDMVKAGFGVNLIGVAVVMLAIMTWGEPLFQLSEFPDWAVLRNVTDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLB2</accession>
    <name>W5NLB2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Leucine rich repeat containing 4C, genome duplicate b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193901.1">
        <property type="nucleotide sequence ID" value="XM_015338415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193902.1">
        <property type="nucleotide sequence ID" value="XM_015338416.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193903.1">
        <property type="nucleotide sequence ID" value="XM_015338417.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193906.1">
        <property type="nucleotide sequence ID" value="XM_015338420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLB2"/>
    <dbReference type="FunCoup" id="W5NLB2">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021421"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021458.1">
        <property type="protein sequence ID" value="ENSLOCP00000021421.1"/>
        <property type="gene ID" value="ENSLOCG00000017316.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682408"/>
    <dbReference type="KEGG" id="loc:102682408"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157405"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_24_1"/>
    <dbReference type="InParanoid" id="W5NLB2"/>
    <dbReference type="OMA" id="NVDEDCG"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017316">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050770">
        <property type="term" value="P:regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000012">
        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF8">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867794" evidence="16">
        <location>
            <begin position="45"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="354"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="464"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="632" mass="70916" checksum="7DB421BE66D676E5" modified="2014-04-16" version="1">MLNKMTSYLQQTMRGPRWNRALSDPLFVLLLALQLLVVAGLVRAQTCPSVCSCSNQFSKVICTRRGLREVPDGISTNTRYLNLQENQIQVIKVDSFKHLRHLEILQLSKNHIRNIEIGAFNGLASLNTLELFDNRLTTIPNGAFEYLSKLKELWLRNNPIESIPSYAFNRVPSLRRLDLGELKRLSYISEGAFEGLSNLRYLNLGMCNLKEIPNLIPLLKLDELELSGNQLSVIRPGSFKGLIHLQKLWMMHAQIQSIERNSFDDLQSLVELNLAHNNLTLLPHDLFTPLHHLERVHLHHNPWNCNCDILWLSWWLKEMVPANTSCCARCHSPSSLKGRYIGELDQNFFTCYAPVIVEPPADLNVTEGMAAELKCRASTSLTSVSWITPNGTIMTHGAYKVRISVLNDGTLNFTNVTVQDTGTYTCMVSNSAGNTTASATLNVSATENSTFSYFTTVTVETIEPSQDEGRTTEQNVGPTATSGWEVPFTTTPLTSTKSTEKTFTIPVTEMGEGSLNGLDEVMKTTKIIIGCFVAITLMAAVMLIIFYKMRKQHHRQNHHAPTRTIEIINVDEEITGGPGLDSHLTLPALEHEHLNHYNSFKTPFNHNSLQSSAHEPLLIRANSKDNVQETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLQ1</accession>
    <name>W5NLQ1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006627928.1"/>
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    </dbReference>
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    <dbReference type="KEGG" id="loc:102682294"/>
    <dbReference type="eggNOG" id="ENOG502QUCY">
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
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        <location>
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            <end position="49"/>
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        <source>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="35589" checksum="CD76D3548991DFE4" modified="2014-04-16" version="1">MEDLSLTGTFLLTLFGEIENVRFAYFALALFVYLSIIVINLTLTLLIFLEKNLHEPMYIFLCGLSVNSLYGTAGFFPRFLADLLSDTQTITRAACFTQAFIIYSYAISEFSMLTVMAYDRYVAITEPLQYNIIMTPRHISVLMAVAWLYPVFCMGLIIMFSARLPLCGNQIERLYCSNWSIVKLSCVNTTLNNAVGFVISCISSVLPLCFILYSYIKILIVCQKSSKEFKNKALQTCLPHLISLVNYSITVLSEVILNRFETKEMLKIVPVILSLEFLIIPPLLNPLIYGLNLPEIRKRIILLFRNKKILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMJ5</accession>
    <name>W5NMJ5_LEPOC</name>
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        </submittedName>
    </protein>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
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    <name>W5NNK3_LEPOC</name>
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        <submittedName>
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                <person name="MacCallum I."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000022212.1"/>
        <property type="gene ID" value="ENSLOCG00000018111.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689299"/>
    <dbReference type="KEGG" id="loc:102689299"/>
    <dbReference type="eggNOG" id="ENOG502S0WC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003866"/>
    <dbReference type="HOGENOM" id="CLU_076106_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNK3"/>
    <dbReference type="OMA" id="RGGNMIL"/>
    <dbReference type="OrthoDB" id="9926693at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018111">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042314">
        <property type="entry name" value="TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="36065" checksum="58CB146908712CB4" modified="2014-04-16" version="1">MVPPPPANKPHVCLSTIIIMSSMALMDAYLVEQNQGPRKIGICIMVMVGDICFLIILRYVAVWVGAEVRTAKRGYAMILWFLYIFVLEIKVYFIYQNYKADRKSLDTIARKALTLLLSICIPALYIILVAIDHMEYVRAFKKKEEIRNRLFWVVVDLLDILDIQANLWEPQKKGLPLWAEGLMFFYCYILLLILPCVSLSEISMQGINIVPHKMMLYPILSLVTINIITIFIRGGNMIFYRDVRVSGILMGKNVLAIILKTCSFVQYRKQLQNAPPAFGIELQKNSVPHNQTLQAPSPVVIQNQTPMPEVTGCEHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP73</accession>
    <name>W5NP73_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkeyp-86h10.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRR15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP73"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022473.1">
        <property type="protein sequence ID" value="ENSLOCP00000022432.1"/>
        <property type="gene ID" value="ENSLOCG00000018331.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S9DE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00760000119737"/>
    <dbReference type="HOGENOM" id="CLU_164864_0_0_1"/>
    <dbReference type="InParanoid" id="W5NP73"/>
    <dbReference type="OMA" id="CRRNMKI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018331">
        <property type="expression patterns" value="Expressed in intestine and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028237">
        <property type="entry name" value="PRR15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14581">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14581:SF4">
        <property type="entry name" value="PROLINE-RICH PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15321">
        <property type="entry name" value="ATAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="115" mass="13345" checksum="833D0372AC6E2710" modified="2014-04-16" version="1">RMAERSTWWKSFTLRKKNKDPAATQYEGNPELQTTQDKTSMGFTSSSDSRENQNQNLIKEQDYEDSNIEPVFNEKTCRRNLKISRSGRFKEKSRVRATLPANNNFFEGSNASVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWK0</accession>
    <name>W5LWK0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="8">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">USB1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">3'-5' RNA exonuclease that trims the 3' end of oligo(U) and oligo(A) tracts of the pre-U6 small nuclear RNA (snRNA) molecule, leading to the formation of a mature U6 snRNA 3' end-terminated with a 2',3'-cyclic phosphate. Participates in the U6 snRNA 3' end processing that prevents U6 snRNA degradation. In addition also removes uridines from the 3' end of U6atac snRNA and possibly the vault RNA VTRNA1-1.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 3'-end uridylyl-adenosine-RNA = a 3'-end 2',3'-cyclophospho-uridine-RNA + adenosine</text>
            <dbReference type="Rhea" id="RHEA:67896"/>
            <dbReference type="Rhea" id="RHEA-COMP:17385"/>
            <dbReference type="Rhea" id="RHEA-COMP:17386"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:85644"/>
            <dbReference type="ChEBI" id="CHEBI:176518"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end uridylyl-uridine-RNA = a 3'-end 2',3'-cyclophospho-uridine-RNA + uridine</text>
            <dbReference type="Rhea" id="RHEA:46052"/>
            <dbReference type="Rhea" id="RHEA-COMP:17384"/>
            <dbReference type="Rhea" id="RHEA-COMP:17385"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:85643"/>
            <dbReference type="ChEBI" id="CHEBI:85644"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:46053"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_015195303.1">
        <property type="nucleotide sequence ID" value="XM_015339817.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LWK0"/>
    <dbReference type="FunCoup" id="W5LWK0">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000507"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000508.1">
        <property type="protein sequence ID" value="ENSLOCP00000000507.1"/>
        <property type="gene ID" value="ENSLOCG00000000465.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688752"/>
    <dbReference type="KEGG" id="loc:102688752"/>
    <dbReference type="CTD" id="79650"/>
    <dbReference type="eggNOG" id="KOG3102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004596"/>
    <dbReference type="InParanoid" id="W5LWK0"/>
    <dbReference type="OMA" id="KTVVLQY"/>
    <dbReference type="OrthoDB" id="49151at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000465">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030223">
        <property type="term" value="P:neutrophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000002">
        <property type="entry name" value="U6 snRNA phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="299" mass="33710" checksum="BC5E35E1B0C90EF9" modified="2014-04-16" version="1">MLVGYSSSSEEEEDSLAVKKRKSETLLNDSASDLKRPKFTPTPLEPGSFQQQESGARGPRRPGESDEVPSPGRHARLPVPGSVLDMFKGSEEEALPDPSRHGGRMRSFGHERGNWATYVYLPYSPDESFLELVEVLARAAQGHGVVLKRADEFHISLSQTVVLRHHWIDPFVLSLKKELANCQKFMCVADRVNVYTNQEKSRTFLGVEVSAGHPQLLEVVRAIDRTLQEFRLSTFYQKPSFHVSLAWCVGDYTETLRGMCCQELQTILDKYEDGAFQLTVPCEDIRCKSGNKVFSFPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWX0</accession>
    <name>W5LWX0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">HIVEP zinc finger 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="7918.ENSLOCP00000000627"/>
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        <property type="gene ID" value="ENSLOCG00000000567.1"/>
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        <property type="entry name" value="Transcription factor HIVEP2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
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            <begin position="192"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXJ5</accession>
    <name>W5LXJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006640493.3"/>
    </dbReference>
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        <property type="interactions" value="1487"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000000852"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000856.1">
        <property type="protein sequence ID" value="ENSLOCP00000000852.1"/>
        <property type="gene ID" value="ENSLOCG00000000772.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693325"/>
    <dbReference type="KEGG" id="loc:102693325"/>
    <dbReference type="CTD" id="9550"/>
    <dbReference type="eggNOG" id="KOG1772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154399"/>
    <dbReference type="HOGENOM" id="CLU_125101_1_1_1"/>
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    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
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    <dbReference type="Bgee" id="ENSLOCG00000000772">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
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        <property type="term" value="P:synaptic vesicle lumen acidification"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="V-type proton ATPase subunit G"/>
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        <property type="entry name" value="V_ATP_synt_G"/>
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        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="V-ATPase_G"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="57"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13478" checksum="3174535BFABDC17C" modified="2014-04-16" version="1">MASQSQGIQQLLQAEKRAAEKVAEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKTKEAAALGSHGNSAVEVDKETLDKMQKIQNSFQQNREAVLASLLSLVCEIKPEIHTNYRING</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYL3</accession>
    <name>W5LYL3_LEPOC</name>
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        <submittedName>
            <fullName evidence="7">Golgi associated kinase 1A</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GASK family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000001055.1"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="FAM198"/>
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        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAM198"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="579" mass="65438" checksum="D0CA3D81D46FC250" modified="2014-04-16" version="1">KAQRFWLKICCRRRSVVAFIFLFTLSVVMINNFPSFPAESYSLSQRERRAGAAGRKGNLRKRASVPFHVLPLNHNHGDWESGKRKEPVEHLADETHEHQPKIPKRAAAAEMTKMKVKASTLDSKGKRQTPQSPFHARGKQGNKHLQEDRKYQNPHVPVRVSPPSRVFPGTAQSALQNLSESLGLLKDKCIPDAHGNAGKAEKFLIPLLANGNLASRNIRKRKTESKPGQAKGPFLSAARRWALGVSRWKKTLSQTMLRTAGRPPPWLSQDDLRKMELLADGVVASRARVPAHGQVLKIGLQQNASQAPGGRDRLCQDGLCALIKRPEDWFEIFAFHLDRVLGLNRSLPAVARNFHSALLPYRFTSGVARPVIWWDPDIQHLADDDNDQNSFPLTWLQYQALLKKKCGNKNPASFTPCVGVRDSEWGKLAIFDFLLQVNDRLDRYCCGFKPDPSEPCVEELLHVKCRNPKELALVHILVRKADPSRLVFIDNAGRPYHPQGNLNFRLLEGIEEIPEGAVSILRSGCLQSMLLRSLYTDKEFWESQGRLKGLKQLIHTVEKRGKLLLQYIKDKNLKLGKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYM8</accession>
    <name>W5LYM8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-set and transmembrane domain-containing protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639495.1">
        <property type="nucleotide sequence ID" value="XM_006639432.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYM8"/>
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        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001235"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001239.1">
        <property type="protein sequence ID" value="ENSLOCP00000001235.1"/>
        <property type="gene ID" value="ENSLOCG00000001095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683618"/>
    <dbReference type="KEGG" id="loc:102683618"/>
    <dbReference type="eggNOG" id="ENOG502RY0S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159381"/>
    <dbReference type="InParanoid" id="W5LYM8"/>
    <dbReference type="OMA" id="NDCINEP"/>
    <dbReference type="OrthoDB" id="9870402at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001095">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902869">
        <property type="term" value="P:regulation of amacrine cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036268">
        <property type="term" value="P:swimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="V-set and transmembrane domain containing 2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF31">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="40"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="24563" checksum="BF6AB7A27E47E0C0" modified="2014-04-16" version="1">MGAFGAIIGILHYMGLYIQLSASSRQAGHAEVENHITGNALFTEVPHDITARSGQDVEMACSFRGAGSPSYSLEIQWWYIRNHKDWTDKQAWTTNQVTPQEEISKDATKISVVKVAGSNISHKLRLSSVKPSDEGTYECRVIDFSDSKARHHKVRAYLQVEPERNLGPHHLRGGPHSQDTKPGSHPSELGKHGPPHSHHKGGRELKKRSADSDNDCTDSCIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYX5</accession>
    <name>W5LYX5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">1-acylglycerol-3-phosphate O-acyltransferase ABHD5</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Abhydrolase domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors. Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37455"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74938"/>
            <dbReference type="ChEBI" id="CHEBI:74941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:37456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = 1-(9Z)-octadecenoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37443"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74928"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:37444"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37131"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:37132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37143"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74551"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:37144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37147"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74552"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:37148"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:33187"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:64839"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:33188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-octadecanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37163"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:37164"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:19710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>eicosanoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-eicosanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37451"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:37452"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01012335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYX5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001332"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001337.1">
        <property type="protein sequence ID" value="ENSLOCP00000001332.1"/>
        <property type="gene ID" value="ENSLOCG00000001181.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694098"/>
    <dbReference type="KEGG" id="loc:102694098"/>
    <dbReference type="CTD" id="566493"/>
    <dbReference type="eggNOG" id="KOG4409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016277"/>
    <dbReference type="InParanoid" id="W5LYX5"/>
    <dbReference type="OMA" id="ARDPIMD"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001181">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010891">
        <property type="term" value="P:negative regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010898">
        <property type="term" value="P:positive regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000019">
        <property type="entry name" value="1-acylglycerol-3-phosphate O-acyltransferase ABHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF34">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ABHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="27">
        <location>
            <begin position="99"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="371" mass="41643" checksum="B30AFE56E31AE201" modified="2014-04-16" version="1">MAEEAMSVERGTAHRVVMNLGIHILYYKFLRFLESTSRSWWITSWLPSWCPTSLSHLKDAEDKMLQSITSKFVKQYVSISNGNVVWTLAFNEGVPEKTPLVLIHGFGGGVGLWVLNLDALCQQRTVYAFDLLGFGRSSRPEFSSDAEEAENQFVDSIEEWRAKVGVETVILLGHNLGGYLAAAYTLRYPSRVKHLILVEPWGFPERPNSPEQDRPIPVWIKALGAMLSPFNPLAGLRLAGPLGPMLVQRLRPDFKRKYASLFDDNTVTEYIYHCNVQTPSGETAFKNMTVPYGWAKRPMLQRVGLIRDDIPIAVIYGARSCIDGNSGSTVKEMRPNSHVETIAVRGAGHYVYADQSEDFNQKVLQICSALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZR2</accession>
    <name>W5LZR2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="4">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts through its C-terminal domain (CTD) with the CTD of eIF1A (EIF1AX) or with the CTD of EIF5 (mutually exclusive) through a common binding site. Interacts with eIF1A (EIF1AX) from the location of the start codon by the 43S complex until the formation of the 80S complex. Interacts with ANXA5 in a calcium and phospholipid-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01025114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001619"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001624.1">
        <property type="protein sequence ID" value="ENSLOCP00000001619.1"/>
        <property type="gene ID" value="ENSLOCG00000001405.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162583"/>
    <dbReference type="HOGENOM" id="CLU_002656_0_2_1"/>
    <dbReference type="InParanoid" id="W5LZR2"/>
    <dbReference type="OMA" id="EFAVMLC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001405">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="18">
        <location>
            <begin position="635"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="36"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="363"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="521"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="236"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="363"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1226" mass="137714" checksum="3DB6FD5A5BC97AAE" modified="2014-04-16" version="1">MGKKQKNKGEDSSKIEGVSFSGILAELLLLGKXKEQDAAKGKGKKKKNKKTQDFDEDDILKELEELSLENQGAKSNKKAEGAEEAAEPGPTKAEKKKTRKGNKKASLDEDEDEGQVEDDVENGVQKPQKTSEPAAPPAASDSEGDDLRPGKKGRGAKKGARKAPPSESDDEDGGAKSRAADPAGDSDEDSEERSRGSRKAQQKSQRAKAGRADSGGEEEDEEEEDGSFKLKTAAQKKAEKKERERKKKEEEKARLRKLKEKDEDKEKSTEGAKKAPVPAAPAPSSEPNAVAEEGEGDKKKKKDKKKKGEKEEKEKEKKKGPSKATVRAMQEALARMKEEEERARQEEEERLRRLEEMEAQRLEQERLEQERKEKKKQKEKERKERLKKEGKLLTKAQREARARAEATLRALQAQGVEVPSKDSVPKKKPVYADKKKKKPSPQTQDEPAEATSPTLEVETAEQAPLEMDTEPLPRDGKAQANERKTETADLDDWEAMASDEEKVTSERNARACLAEMKKVHIEVKEQQQQEEEEDEEEDASEEEEEEEEEEDEESEEEEEEEEGSKVGAPVEKETPLPPAKRVRNRRTSRDVSSGTDSECDSDDGRTKEEQRYDRAKRRIEKRRAENLRNINMEKLRSPVICVLGHVDTGKTKILDKLRHTHVQDSEAGGITQQIGATNVPLETIVEQTKMVKNFDRENIKIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTLESINLLKEKKCPFIVALNKIDRLYDWRKSPETDVVTTLKKQKKNTKDEFDERAKAVIVEFAQQGLNATLFHENKDPRTFVSLVPTSAHTGDGMGNLIGLLVELTQTLLTRRLAHSDELRAQVMEVKALPGMGTTIDVILINGCLREGETIIVPGVEGPIVTQIRGLLLPPPLKELRVKNQYEKHKEVSTAQGVKILGKDLEKTLAGLPLLVAHREDEIPVLRDELVRELKQTLSSIKLEEKGVYVQASTLGSLEALLEFLRTSKVPYAGINIGPVHKKDVMKASTMLEHDPQYAVILAFDVKVERDSQELADSLGVRIFSAEIIYHLFDAFTKYREDYKKQKQDEFKHIAVFPCKLRILPQFIFNSRDPIVMGVTVEAGVVRQGTPVCVPSKSFVDIGIVTSIEINHKPVDVAKKGQEICVKIEPIPGESPKMYGRHFEATDFIVSKITRQSIDALKDWFRDEMQKSDWQLIMHNSAAFYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZY8</accession>
    <name>W5LZY8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">DIX domain containing 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DIXDC1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000001695.1"/>
        <property type="gene ID" value="ENSLOCG00000001475.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG26"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="entry name" value="Calponin-like domain"/>
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        <property type="entry name" value="CH_dom"/>
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        <property type="entry name" value="DIX"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <begin position="20"/>
            <end position="127"/>
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        <location>
            <begin position="500"/>
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            <end position="190"/>
        </location>
    </feature>
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            <end position="225"/>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
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    <sequence length="577" mass="64004" checksum="29CFA89ED656D4A9" modified="2014-04-16" version="1">MIASLSRGNLLDVLQEGFNEQQLQAYVSWVNSQLRRRPGLQPVQDLRRDLRDGVVLAHLVEIVAGELLEGVHYSPADPQEMRENVEKVLQFVASKRIHLNNHSSKFWSIKNISTSLTLAFALTTHRQPATRLVGDVHSVPGRHQAQGHHTPESFWIPAATGLAGGEPLRSGSSASSARRRSSSVDEEIENPSWSVRALVQQYEGQREAPAGDSEALPSGLSSPSPAHTPKGELAVGQSESQEAKLSAQLEEARCKAEVLVMAPLGGPVGAGEGSSWEDQLLEQQEQLEREMEEARRMISSLQKKRIVINQAAANQKQSPLETEENNIRIPLFVIINKNLNVSFKKNRKPILHDSDRPLGLSLSQDALQQRMSLQESSVLQLKQELLRASMARDELHSQNISIVRIVDKQSTNLAQAKKELGHKDRLLQQQQLKLDEALRKVAERAEEQAARGSLSPSLCACVPQSRGKSPGRRAAQSDRDSWPPSSKMSHSHSSPVLSSSATTKVLYFTDRSLTPFMVNIPKRLGEVTLRDFKAAVDREGSYRYHFKALDPEFGTVKEEVFHDDAVVPGWEGKIVAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1L3</accession>
    <name>W5M1L3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Leucine rich repeat and fibronectin type III domain containing 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cys-rich_flank_reg_C"/>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865490" evidence="18">
        <location>
            <begin position="30"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="543"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="298"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="434"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="395"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="605"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="666"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="625"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="776" mass="84442" checksum="374DE6D670012BB6" modified="2014-04-16" version="1">MRRPLLAPAGTALLLLLLLLAPAPGPSAACPFHCVCQNLSESLSTLCANKGLLFVPPNIDRRTVELRLADNFIREVGQEDFLNMSGLVDLTLSRNTIHLIRPLAFADLESLRSLHLDANRLTEVRAQDLRGLLNLQHLILNGNQLTDISARAFDDFLLTLEDLDLSYNNLRKVPWEAIQSMASLHTLNLDHNLIDHVMEGSFAELYKLARLDMTSNRLQTLPPDPLFARSQTGVVSPTPYNAVVSLNFGGNPLHCNCELLWLRRLAREDDMETCATPPHLAGRYFWSIPEEEFTCEPPLITRRTHKLWVLEGQRATLRCRAIGDPEPMMHWVSPDDRIISNSSRLASYRNGTLDILVTSVRDDGAYTCIAINAAGESTALVDLKIIPLPHRANGTAPVLHRDPGSSDITTSAKTGGVQGGGGGNGTGPEGGARLPERLVGVMDVTSTSAEVRWYVGKSSSYLVWMYQIQYNCTVDETLVYRILPSTSNNFLLKNLVAGVDYDLCVLAVFDDLATSLAATKVLGCAQFSTKDDYVHCHSLHAHFLGGTLTVIVGGVIVVTLLVFTVVMMVKYRICASAHAPLPKVTDVYSQTNGGNAPLPNGLLLRRGSLAPLPKPPPPQQLVAAADRRGPPRTPEQERAALYYGTRPQRRRGKPKYRGEGGRVGAKLALPALPPPAGEARDYGKEPRQAAPARAAGAGRGHPGCWNPPAKAKRSCSFDMGEIAATTCYSYAKRLSVIWTRRSQSVHGVLMHCAEAADLAGNALALASADELEESVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2D3</accession>
    <name>W5M2D3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2D3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002541"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002547.1">
        <property type="protein sequence ID" value="ENSLOCP00000002541.1"/>
        <property type="gene ID" value="ENSLOCG00000002174.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4686">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165198"/>
    <dbReference type="HOGENOM" id="CLU_024694_0_1_1"/>
    <dbReference type="InParanoid" id="W5M2D3"/>
    <dbReference type="OMA" id="TWTLWGG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002174">
        <property type="expression patterns" value="Expressed in embryo and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF5">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="21">
        <location>
            <begin position="11"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="441"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="479" mass="52445" checksum="913E36A53BABF1CC" modified="2014-04-16" version="1">MGKPAEKAFYRFLVLFFNCLLTFGSYFCFDIPSVLQDQFQGNLTCPNAMVANGSAPDGTGECVEGLGMTPQEYNLLYAIYAWTNAVVVILAGFLIDKLGNRSGVFLFSFLTVLGSAVFALGSHFKGTSYLLPLMLTGRLLFGSGNGSLSIVQNRITAFWFKGKELALAFGLTVAFSRLGSVLNFFFTQRVEAHFGLQWTLWGGTFLCVLGFLAAITVSTLDKVGMRQLGLDGAIQEESRKVRVQNVRQLSLRYWLLVLTIMFFYNGIFPFIADASGFWQKPHVWYSLMHAVLLCLQLVLSAAVGILIDHIGLRGVFAVGCAVLTLPVFALLAFTFVPPLVSTVWLGITYSFAAASMWPSIPLVVPRATLGTAMGLVTSVQMIGIGISNLIVGQILGTKSSDVKIPLWRWQQMMVFMLANTIACIVTSVVLNVVDQRQGGTLNKTTKRSSEAPREPPAHSSETSPLLEEEDTIRFQPIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3J3</accession>
    <name>W5M3J3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Somatolactin beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the somatotropin/prolactin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192991.1">
        <property type="nucleotide sequence ID" value="XM_015337505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3J3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002951"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002956.1">
        <property type="protein sequence ID" value="ENSLOCP00000002951.1"/>
        <property type="gene ID" value="ENSLOCG00000002505.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687524"/>
    <dbReference type="KEGG" id="loc:102687524"/>
    <dbReference type="eggNOG" id="ENOG502R5GJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182818"/>
    <dbReference type="HOGENOM" id="CLU_088274_2_1_1"/>
    <dbReference type="InParanoid" id="W5M3J3"/>
    <dbReference type="OMA" id="ERYDHAP"/>
    <dbReference type="OrthoDB" id="9945472at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002505">
        <property type="expression patterns" value="Expressed in brain and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070186">
        <property type="term" value="F:growth hormone activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060396">
        <property type="term" value="P:growth hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031667">
        <property type="term" value="P:response to nutrient levels"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000042">
        <property type="entry name" value="Somatolactin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001400">
        <property type="entry name" value="Somatotropin/Prolactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018116">
        <property type="entry name" value="Somatotropin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417:SF3">
        <property type="entry name" value="SOMATOLACTIN ALPHA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417">
        <property type="entry name" value="SOMATOTROPIN,PROLACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00103">
        <property type="entry name" value="Hormone_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00836">
        <property type="entry name" value="SOMATOTROPIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00266">
        <property type="entry name" value="SOMATOTROPIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00338">
        <property type="entry name" value="SOMATOTROPIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="27324" checksum="EC317649CB079BF5" modified="2014-04-16" version="1">MQKVKDMSVCVVLQGCVWAILLWPAWGALGYPLDCKDEQGSISRCSSISLEKLLDRVIQHAELIYRVSEESCTLFEEMFVPFTMHSQKIRGSSSCVTKAFPIPGSKSEIQQISDKWLLHSVLMLVQSWMEPLVYLQTTLDRYDDAPDTLLNKTKWVSDKLPSLEQGIVVLIRKMLDEGMLTSDYQLTLGHYDVQPEVVESVLRDYALLTCFKKDAHKMETFLKLLKCRRTDKLSCFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4W0</accession>
    <name>W5M4W0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01037292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4W0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003418"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003425.1">
        <property type="protein sequence ID" value="ENSLOCP00000003418.1"/>
        <property type="gene ID" value="ENSLOCG00000002901.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="HOGENOM" id="CLU_002274_3_2_1"/>
    <dbReference type="InParanoid" id="W5M4W0"/>
    <dbReference type="OMA" id="IFRRECQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002901">
        <property type="expression patterns" value="Expressed in pharyngeal gill"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NOD1/2 winged helix" evidence="8">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT LRR and PYD" evidence="7">
        <location>
            <begin position="176"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="41068" checksum="D69423FC772FF16E" modified="2014-04-16" version="1">IWITSRPAAAGLIPPELISRVTEVQRFDDKQKEEYIRKNCKNRTLANRIISLIKTLRSLYMLCYVPVLCWIVVTVLEHTLEKNSEESSKTLTDLYTNFLLVLLTARKGKRTNQEAILKVGNLAFESLEKNITVFYEKDLREYGININNSSLYSGVCKDIFKQDSVLFEEKVYSFLHLTVQEYFAALYVFGSYHNNYFNPLKKYKKYKDSTLKRKPKLTLNKNALERAIQSSNGHLDFFVRFLLGTEMENNQKLLKGFLSHSQDYPSDIQKTAEHIKKLIKKTSSPERCMNLFHCLSELKDQFLLDEFNVTLDAVKHSGQSLSPSQCSALAYFSLLSEKEIDVFDMSKYATSEECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5A2</accession>
    <name>W5M5A2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNRF3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01005667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5A2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003560"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003567.1">
        <property type="protein sequence ID" value="ENSLOCP00000003560.1"/>
        <property type="gene ID" value="ENSLOCG00000003019.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154006"/>
    <dbReference type="HOGENOM" id="CLU_023160_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5A2"/>
    <dbReference type="OMA" id="PAVHFHQ"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003019">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038018">
        <property type="term" value="P:Wnt receptor catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000023">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF43 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000970">
        <property type="entry name" value="Ring finger protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040700">
        <property type="entry name" value="ZNRF-3_ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18212">
        <property type="entry name" value="ZNRF_3_ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="337"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="480"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="617" mass="67281" checksum="5CEA5ACE904FD311" modified="2014-04-16" version="1">PSLSFSAQAQLALDRGAQAVIFDVTDDATAAAELRDPRGLPRPVVLVQARDAEVLMGLVNRNEEAMVQIEVKIEPNKWPHYDVGILLTVVLAMLAIIIIFAFRFKCKQNRNQPTQDSIHQQTVRAINRLGTRTYTSQGRSRNQRTRGGRDSGSSSTSTPVCAICLEEFLDGQDLRIISCSHEFHKECVDPWLLQHRTCPLCVHNIMETDGVPQSAHPSRHQQNMEHNQRVLLLHHYPRHPPLHQHPIAIPVHHYPRPPTGQLGHYGISPPLDPRALRCVPSRHLGAGPGRCSYHRNEGLLGHYHRTSTACRPPANPEPHCQPRSCAPAYCPSCAAPRGGSSSRLPFAPGRGAAPSRQDDGSCSGGSYRTERSGYLADGPASDSSSGPCHGSSSDSVLNCTDVSLQGVYGSWSTFRSSLSSDYDPFVYCGQGQGQGQGQGRGAGDSRDVGARPRSLDSMVNRTCPEEKVFSHVHYHRHRHHHYDEVDRGQGPDRGSDEELNAGAAPVPDSMIPEADPSRCQPCQCPKPAPDPHRADVPESELPADACSGTATLPPPAPQPPCCHHGPSRHHRRKKGGVPDGPAVHFHQSLDTQEDCSIYVHYGQGAGYCCPPDMPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5I6</accession>
    <name>W5M5I6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635625.1">
        <property type="nucleotide sequence ID" value="XM_006635562.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5I6"/>
    <dbReference type="FunCoup" id="W5M5I6">
        <property type="interactions" value="1208"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003644"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003651.1">
        <property type="protein sequence ID" value="ENSLOCP00000003644.1"/>
        <property type="gene ID" value="ENSLOCG00000003094.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688389"/>
    <dbReference type="KEGG" id="loc:102688389"/>
    <dbReference type="CTD" id="9694"/>
    <dbReference type="eggNOG" id="KOG3060">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011922"/>
    <dbReference type="HOGENOM" id="CLU_052388_1_0_1"/>
    <dbReference type="InParanoid" id="W5M5I6"/>
    <dbReference type="OMA" id="MSDQEGW"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003094">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000074">
        <property type="entry name" value="ER membrane protein complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="9">
        <location>
            <begin position="90"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="34426" checksum="9E5F6324E601EA35" modified="2014-04-16" version="1">MASVSELYDVTWEDMRDKLRKWREENYRNSEQIVEVGEELIHEHMSKLGDDIWIIYEQVMIAALDCSRDDLALSCLHELRKQFQGSHRVKRLAGMRLEALERYDEACKVYDTILQDDPTNTAARKRKIAILKAQGRSTEAIRELNEYLEQFVGDQEAWHELAELYINEHDYAKAAFCLEELMMTNPHNHLYCEQYAEVKYTQGGIENLELSRKYFAQALKLNNRNVRALFGLYMSASHIAANPKVSAKVKKDNVKYAAWAASQINRAYQLAGRGTKETKCSLKAVEEMLETMQITQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5L0</accession>
    <name>W5M5L0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="13">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase that plays a role in the regulation of R-loop homeostasis in both endogenous R-loop-prone regions and at sites of DNA damage. At endogenous loci such as actively transcribed genes, may act as a helicase to resolve the formation of R-loop during transcription and prevent the interference of R-loop with DNA-replication machinery. Also participates in the removal of DNA-lesion-associated R-loop. Plays an essential role for establishing pluripotency during embryogenesis and for pluripotency maintenance in embryonic stem cells. Mechanistically, prevents the polycomb repressive complex 2 (PRC2) from accessing rDNA loci and protects the active chromatin status in nucleolus.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with NOL8; the interaction is RNA-dependent. Interacts with PRC2 complex components EZH2, SUZ2 and JARID2; these interactions prevent deposition of the repressive H3K27me3 mark onto rDNA in pluripotent cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01011561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214126.1">
        <property type="nucleotide sequence ID" value="XM_015358640.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5L0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003668"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003675.1">
        <property type="protein sequence ID" value="ENSLOCP00000003668.1"/>
        <property type="gene ID" value="ENSLOCG00000003108.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693706"/>
    <dbReference type="KEGG" id="loc:102693706"/>
    <dbReference type="CTD" id="8886"/>
    <dbReference type="eggNOG" id="KOG0342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00680000100037"/>
    <dbReference type="HOGENOM" id="CLU_003041_26_5_1"/>
    <dbReference type="InParanoid" id="W5M5L0"/>
    <dbReference type="OMA" id="LMEFHSQ"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003108">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000460">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="203"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="392"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="664" mass="74917" checksum="7E3B2A45323C8B6B" modified="2014-04-16" version="1">MADLQMKLLRKKIQKRNEKNKKRNLLKKQRKEEEDDDCGGKLAAPCPDAEDERESGQERDTVKPAKKLKKTQRVSADEDAVAQGPRKKKRKIKLTNNSDGTETKKAKQEEEQEETEGEASGEVVTSVDGLEEPEQSDEEEQAAIAESEEDDEEGDGPQLPLGLTGAFEDTSFASLSGAVSEHTLKGVKEMGFEHMTEIQHKSIRPLLEGRDVLAAARTGSGKTLAFLIPAIELIYKLKFMPRNGTGVVILSPTRELAMQTYGVLKELMTHHVHTYGLIMGGSNRSAEAQKLANGINIIVATPGRLLDHLQNTPGFMYKNLQCLIIDEADRILEVGFEEELKQIIKLLPKRRQTMLFSATQTRRVEDLARISLKKEPLYVGVDDNKETATVEGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKFHYELLNYIDLPVMAIHGKQKQTKRTTTFFQFCNADSGILLCTDVAARGLDVPEVDWIVQYDPPDDPKEYIHRVGRTARGIDGRGHALLILRPEELGFLRFLKQAKVPLSEFEFSWSKISDIQSQLEKLIEKNYYLHKSAQEAYKAYVRAYDSHSLKQIYSVNTLDLQKVALSFGFKVPPYVDLNVNSSRGVKMQKRGGGGGFGYQKSKNVYKSKIFKHVSKAGADKRQFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5U7</accession>
    <name>W5M5U7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neurturin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Growth factor that supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Involved in the development of the neural crest. Might control the size of non-neuronal cell population such as haemopoietic cells. Acts by binding to its coreceptor, GFRA2, leading to autophosphorylation and activation of the RET receptor. Heparan sulfate-binding is required for signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer; disulfide-linked. Interacts with GFRA2 coreceptor and RET: forms a 2:2:2 ternary complex composed of NRTN ligand, GFRA2 and RET receptor. Also forms a 4:4:4 tetrameric complex composed of 4 copies of NRTN ligand, GFRA2 and RET receptor, which prevents endocytosis of RET.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5U7"/>
    <dbReference type="FunCoup" id="W5M5U7">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003755"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003762.1">
        <property type="protein sequence ID" value="ENSLOCP00000003755.1"/>
        <property type="gene ID" value="ENSLOCG00000003179.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWH4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182993"/>
    <dbReference type="InParanoid" id="W5M5U7"/>
    <dbReference type="OMA" id="WHTSSSR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003179">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035860">
        <property type="term" value="P:glial cell-derived neurotrophic factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19383">
        <property type="entry name" value="TGF_beta_Neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000037">
        <property type="entry name" value="neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF3">
        <property type="entry name" value="NEURTURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="114"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="220" mass="24565" checksum="8A56D81138D97E90" modified="2014-04-16" version="1">MLCGAALSVFLCRNMIPGGEGPGAWPSSSSSTISSSWLKGHHRAVRAADKPSLFRECYGLSDKGRKSAGNKSYRSYISYLLQSYTEGEVKQLISALVDRSGPEDSRLDLSLSKRTKRARKGLLKPCSLKELEVSVSQLGLGYEGDEILLFRYCSGRCQEARRNYDLTLEHMKKQGRVKKGKARHKPCCRPTEYDDDISFLDNGNKYHTIREVSARKCGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6K9</accession>
    <name>W5M6K9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 is involved in the regulation of cell adhesion and cell migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. Involved in somitogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 32">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01009721">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6K9"/>
    <dbReference type="FunCoup" id="W5M6K9">
        <property type="interactions" value="581"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004017"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004025.1">
        <property type="protein sequence ID" value="ENSLOCP00000004017.1"/>
        <property type="gene ID" value="ENSLOCG00000003331.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155503"/>
    <dbReference type="HOGENOM" id="CLU_000288_141_0_1"/>
    <dbReference type="InParanoid" id="W5M6K9"/>
    <dbReference type="OMA" id="GAINCIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003331">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10472">
        <property type="entry name" value="EphR_LBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09552">
        <property type="entry name" value="SAM_EPH-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000004">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000110">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF11">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 22 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="29">
        <location>
            <begin position="24"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="328"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="439"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="626"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="27">
        <location>
            <begin position="920"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="751"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="632"/>
            <end position="640"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 24">
        <location>
            <position position="658"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="66"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="993" mass="110953" checksum="3407F8E289605420" modified="2014-04-16" version="1">QNLITVLSDCSCFIQTLVTVFVFSEMLMDSTTATAELGWTVYPSTGWEEVSGYDENMNTIRTYQVCNVFETNQNNWVRTKYIRRRGAQRIHVEMKFSVRDCSSIPNVPGSCKETFNLYYYESDTDSVSKSSPAWMENPWIKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVKVFYRKCPRVIQNGAIFQETLSGAESTSLVTARGTCVPNAEEVDVPIKLYCNGDGEWMVPIGKCMCKAGHEAVENGTVCKACPSGSFKPAQGDDSCMQCPINSRTTSEGAVSCVCRSGHYRTETDPLEMPCTTIPSAPQNVISSVNETSLMLEWSPPREAGGREDVVYNIICKSCGAGRGACTRCGDNVQFVPRQLGLTEPRVYISDLLAHTQYTFEIQAVNGVSDQSPYSPQFSAVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKDQSEYNSSTVRSQTNTAVIRGLKPGTIYVFQVRARTVAGFGRYSGKMYFQTMTEEEYKTSLQEKLPLIIGSAAAGLVFLIAVVVIIIVCNRRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGNLKLPGKREILVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSSPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSDMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALLKGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNNRPKFGQIVNTLDKMIRNPNSLKAMTPLSSGVHLPLLDRSVPDFSSFNTVDEWLDAIKMGQYKENFANAEFTSFDVVSQMTMDDILRVGVTLAGHQKKILNSVQMMRAQMNQIQSVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6T0</accession>
    <name>W5M6T0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LIM homeobox 8a</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634822.2">
        <property type="nucleotide sequence ID" value="XM_006634759.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6T0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004088"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004096.1">
        <property type="protein sequence ID" value="ENSLOCP00000004088.1"/>
        <property type="gene ID" value="ENSLOCG00000003447.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696063"/>
    <dbReference type="KEGG" id="loc:102696063"/>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156200"/>
    <dbReference type="HOGENOM" id="CLU_027802_1_0_1"/>
    <dbReference type="InParanoid" id="W5M6T0"/>
    <dbReference type="OMA" id="DPMFVCK"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003447">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021884">
        <property type="term" value="P:forebrain neuron development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09381">
        <property type="entry name" value="LIM1_Lhx7_Lhx8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09379">
        <property type="entry name" value="LIM2_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000050">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000031">
        <property type="entry name" value="LIM homeobox 6, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF117">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="153"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="242"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="244"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="41847" checksum="028EC0200615EF7E" modified="2014-04-16" version="1">MYWKGEQMLVCSKAEDKGNALNAVSSETFLVMSEDCEGAATGSFGRCRKPRTETELSTPEEIFEDDSCSQSSSLSPSSSPQAMTSASLLGKSICTSCGLEIVDKYLLKVNDLCWHVRCLSCSVCRTSLGRHTSCYIKEKEVFCKLDYFRRYGTRCARCGRNIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRTHYDCMLDNLKRAVENGSGVNVEGAMPTEQEVNQPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSTAPVSTMQPSRLSPPMMEELQYSAYVPPDGPMLTALHSYMDAHSPSSLVFPPLLPHSMAQLPISHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7U6</accession>
    <name>W5M7U6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="29">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 29">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Golgi apparatus</location>
            <location evidence="28">trans-Golgi network membrane</location>
            <topology evidence="28">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Membrane</location>
            <topology evidence="29">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Presynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Synapse</location>
            <location evidence="26">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 29">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="29"/>
    <dbReference type="EMBL" id="AHAT01025241">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7U6"/>
    <dbReference type="FunCoup" id="W5M7U6">
        <property type="interactions" value="1446"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004454"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004462.1">
        <property type="protein sequence ID" value="ENSLOCP00000004454.1"/>
        <property type="gene ID" value="ENSLOCG00000003739.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691994"/>
    <dbReference type="KEGG" id="loc:102691994"/>
    <dbReference type="CTD" id="55361"/>
    <dbReference type="eggNOG" id="KOG2381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010434"/>
    <dbReference type="HOGENOM" id="CLU_032516_1_0_1"/>
    <dbReference type="InParanoid" id="W5M7U6"/>
    <dbReference type="OMA" id="IKCDCPL"/>
    <dbReference type="OrthoDB" id="3349449at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003739">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="24" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="21 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="12 29" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="31">
        <location>
            <begin position="92"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00037864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="447" mass="51628" checksum="D71664941792560A" modified="2014-04-16" version="1">MDETSPLVSPLRDPPDFSYCPTEPMSPRAEFGATPGSVVRVPAGSPGRSRERQPLLERDRGYSPRDPYRNEFPEDPEFREIIRKAERAIEEGIYPERIYQGSSGSYFVKDSQGKIIGVFKPKNEEPYGQLNPKWTKWLQKLCCPCCFGRDCLVLNQGYMSEAGASLVDHKLELDIVPRTKVVHLASETFNYSAIDRVKSRGKRLALEKVPRVGQRFHRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLQLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDTGNSRDADWVVVKEPIIKIAAIDNGLAFPLKHPDSWRAYPFYWAWLAQAKVPFSQEIKDHVLPKISDPNFVKDLEEDLYELFKKDPGFDRGQFQKQIAVMRGQILNLCQAMKDGKTPLQLVQMPPVIVETARAPQRANSESYTQSFQSRRPFFTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8B7</accession>
    <name>W5M8B7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GABA(C) receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627815.1">
        <property type="nucleotide sequence ID" value="XM_006627752.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069046018.1">
        <property type="nucleotide sequence ID" value="XM_069189917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8B7"/>
    <dbReference type="FunCoup" id="W5M8B7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004625"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004633.1">
        <property type="protein sequence ID" value="ENSLOCP00000004625.1"/>
        <property type="gene ID" value="ENSLOCG00000003872.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698397"/>
    <dbReference type="KEGG" id="loc:102698397"/>
    <dbReference type="eggNOG" id="KOG3643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159906"/>
    <dbReference type="HOGENOM" id="CLU_010920_0_1_1"/>
    <dbReference type="InParanoid" id="W5M8B7"/>
    <dbReference type="OrthoDB" id="442503at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003872">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <sequence length="467" mass="54143" checksum="221260E7E1AAA77E" modified="2014-04-16" version="1">MDAMHLAFRLLFLSWLWPGALLSGSHFQGKKKNKDVVLGEHRKHGSRIDFKLKKSDSTKSTLIKSEQLLRIEDHDFAMRPGFGGTAIPVGIDVQVESIDSISEVNMDFTMTLYLRHYWKDERLAFPSSNNKSRTFDSRLVKKIWVPDVFFVHSKRSFIHDTTMENIMLRVYPDGNILYSVRITVTALCSMDFSRFPLDTQNCSLELESYAYNENDLMLYWKNGNDSLRTDEILLSQFFIEEFNPSNGLAFYSSTGWYNRLYINFILRRHIFFFMLQTYFPTMLMVMLSWVSFWIDRRAVPARVSLGITTVLTMSTIITGVSASMPQVSYVKAVDIYLWASFLFVFLSVIEYAAVNYLTTIEEMKKMEKGKLPVTFNTTQAMAFDGCFHDNEIELTPFPEIPVNPEQPPARNSADAQPVEGHRLRRKRSIKSNVNYIMNNSHVIDSYSRLLFPAAYLLFNIIYWGLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M936</accession>
    <name>W5M936_LEPOC</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Lepisosteidae</taxon>
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        </lineage>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000004099.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG12"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:structural constituent of eye lens"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="21456" checksum="55495B8EBADC92AB" modified="2014-04-16" version="1">ALPLVSLLPQIIFYEDKNFQGRRYECDSDCSDFHTYLSRCNSIRVESGAWVLYERPNYMGYQYVLTRGEYPEYQRWMGLSDRLGSCKMIHFPSGTQYKIQLYDKGDFTGQVFEATEDCPSVLERYRTREVHSCKVLEGVWVFYENPNYRGRQYLLEKGEYHKPVEWGAVCPTVLSFRRITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9K9</accession>
    <name>W5M9K9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 36a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9K9"/>
    <dbReference type="FunCoup" id="W5M9K9">
        <property type="interactions" value="1853"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005068"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005076.1">
        <property type="protein sequence ID" value="ENSLOCP00000005068.1"/>
        <property type="gene ID" value="ENSLOCG00000004239.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687656"/>
    <dbReference type="KEGG" id="loc:102687656"/>
    <dbReference type="eggNOG" id="KOG0757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154369"/>
    <dbReference type="HOGENOM" id="CLU_015166_6_0_1"/>
    <dbReference type="InParanoid" id="W5M9K9"/>
    <dbReference type="OMA" id="WVMYEQM"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004239">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000074">
        <property type="entry name" value="Solute carrier family 25 member 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000124">
        <property type="entry name" value="Solute carrier family 25 member 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="118"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="226"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="313" mass="34536" checksum="7653B9B9969A6E43" modified="2014-04-16" version="1">MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLSTVNGASVNRVARVSPGPLHCLKLILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNAKEKLNGILEPDSTQVHMISAGLAGFTAITATNPIWLIKTRLQLDARNRGERRMSAFECIRKVYQSDGIRGFYRGMSASYAGISETVIHFVIYESIKRKLLQCKTASNMDDEDESVKDASDFVGLMLAAATSKTCATSIAYPHEVIRTRLREEGTKYRSFFQTLSMVMREEGYRALYRGLTTHLVRQIPNTAIMMSTYELVVYLLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9Z9</accession>
    <name>W5M9Z9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation. Plays a role in neuronal migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2. TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 assemble in a 5:5:2:1:1 stoichiometry; each is associated with a gamma-tubulin, thereby arranging 14 gamma-tubulins in a helical manner. Gamma-tubulin at the first position is blocked by TUBGCP3 at the last position, allowing 13 protafilaments to grow into a microtubule. The gTuRC (via TUBGCP3 and TUBGCP6) interacts with ACTB and MZT1; the interactions form a luminal bridge that stabilizes the initial structure during complex assembly. The gTuRC (via TUBGCP2) interacts with MZT2A/MZT2B and CDK5RAP2 (via CM1 motif); the interactions play a role in gTuRC activation. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9Z9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005208"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005216.1">
        <property type="protein sequence ID" value="ENSLOCP00000005208.1"/>
        <property type="gene ID" value="ENSLOCG00000004354.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156697"/>
    <dbReference type="HOGENOM" id="CLU_007738_1_0_1"/>
    <dbReference type="InParanoid" id="W5M9Z9"/>
    <dbReference type="OMA" id="QNMSGDP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004354">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000002">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="11">
        <location>
            <begin position="169"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="10">
        <location>
            <begin position="460"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="729"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="836" mass="96370" checksum="C5511159A79C49BC" modified="2014-04-16" version="1">SLQVKIAEFSKTPEDFLKKYDELKSRNVRNVDPLVYLLSKLTEDKETLQCLQQNAKERSEQSASASSSSTNFSIPATSTKMSMQELEELRKKLGNVAASSSVQQPSEVTRKMLRDRHNKKSPLQPIPVFPNWVYDRPALLGDFITGSSGTDSAVPIGTLPLAAQEQALVEDLLYLLIGVDGRDITAQPVIGRQSRAFSIDATLDVSIKELVNRILPVAASYSTVTRFIEEKSSFEYGQVNHALTAAMRTLMKEYMILVTQLEHLQRQGMLSLQKLWFYIQPTMRTMEILASIATSIDKGDCMGGSTLSLLHDRTFNYTGDSQAQELCLYLTKAASVPYFEILEKWIYRGIIKDPYSEFMVEEHELQKEKIQEDYNDKYWDQRYTIVQLRIPSFLQKMADKILSTGKYLNVVRECGRDVTCPDAREVLYTLKERAYVEQIEKAYNYASKVLLDFLMQEKELVTRLRSIKHYFLMDKGDFFVHFMDLTEEELKKPVDDIIPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDVITQLLRVLAIETKQEKAIINADPTEISLSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERLLCNVWISNKTAKQYSLHSAKWFAAAFTLRQRMLNFVQNIQYYMMFEVMEPTWHIMENNLKSASNIDDVLCHHTSFLDNCLKDCMLTNPELLKIFSKLMSVCVMFTNCMQRFTQSMRVDSEMSRLSLEHGTMSGPPSQSERSEEAEKKRLTSKYLAEHADALQSSSGFEATISQFDNNFSTHLLDLLDKLSIYSTNDCEHSMINIIYRLDFNGFYTERLERMSIERSQKAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAW0</accession>
    <name>W5MAW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018329">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018330">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAW0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005519"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005527.1">
        <property type="protein sequence ID" value="ENSLOCP00000005519.1"/>
        <property type="gene ID" value="ENSLOCG00000004603.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164612"/>
    <dbReference type="HOGENOM" id="CLU_002568_3_0_1"/>
    <dbReference type="InParanoid" id="W5MAW0"/>
    <dbReference type="OMA" id="VDQVPFD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004603">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000005">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000040">
        <property type="entry name" value="macrophage-capping protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF27">
        <property type="entry name" value="SCINDERIN LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="277"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="431"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="5">
        <location>
            <begin position="532"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="628" mass="69432" checksum="FA6AAD7221D0D73F" modified="2014-04-16" version="1">SPRRALSSLSDQASRDRVSGCLTMVSHKEFESAGKQPGLQIWRIENMDLKPVPKPLHGSFFTGDSYIVLQTTAAPSYNIHMWLGEWASALSSHAQTRRLRLFPVSCTRRFPALLECLLGYLSQMHKKFQIGGVASGFKHVVTNDANVKRLLHIKGRRIIRATEVDLSWGSFNKGDCFIIDLGKDIYQWCGSECNRFERLKASQVAIDIRNNERNGRAKITMVEEDAEPQEIIAILGAKPGIPPGGPDDEAVETANKNKGSLYMVSDATGSMKTSLVAQSSPFKQAMLSPDECYILDNGVDNKLFLWKGSNANTGERKAAMKTAEQFIKDKNYSSKTQMRLRRTSNLSHAGSKSFKKQQFYFDSHFHQHGLLNTKPVYWNGFSARSYCIREKHKKCTDRKSSACVNLPVLGSHPALSRTKSGGAEPQPPFSPQVKACASSLNTNDVFVLKAPDATYVWRGVGATEEEVAAAKHVVAMLKGSASEVSEGKEPAGFWAALGGKKEYQTSRSLQKAVTPPRLFGCSNKTGRLVAEEVPGDFTQADLATDDVMLLDAGDQIFLWIGNEANDVEKTGAPKIAKEYVDSDPSGRRGIPVTTVKQGHEPPTFTGWFMAWDSNMWDTDPLDRIRAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB98</accession>
    <name>W5MB98_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death 6-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Midbody</location>
            <location evidence="1">Midbody ring</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB98"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005657"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005665.1">
        <property type="protein sequence ID" value="ENSLOCP00000005657.1"/>
        <property type="gene ID" value="ENSLOCG00000004712.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163083"/>
    <dbReference type="HOGENOM" id="CLU_007181_2_0_1"/>
    <dbReference type="InParanoid" id="W5MB98"/>
    <dbReference type="OMA" id="VSHAEEM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004712">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090543">
        <property type="term" value="C:Flemming body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09240">
        <property type="entry name" value="BRO1_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09235">
        <property type="entry name" value="V_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000001">
        <property type="entry name" value="programmed cell death 6-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="5">
        <location>
            <begin position="7"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="724"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="853"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="779"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="862"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00074846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="884" mass="98187" checksum="2ACA949F27C00CB4" modified="2014-04-16" version="1">SSPRMASFISVPLKKSSEVDLVKPLSKFISSTYSAGEEQAEYIRGVEELNKLRKTALGRPLDKHESSLEILLRYYDQLCAIEPKFPFSENQLCLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNIGALASQIASEQNLDNDEGLKAAAKFYQLASGAFSHIKDTVLSALNREPTMDISPDTVGTLSHIMLAQAQEVFFLKATSDKMKDAIIAKLANQAAEFYGDAFKQCQYKENLPKYFYFQEVLPILAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAADLVKTVASRYDEYVNVKDLSDKISRALTAAKKDNDFIYHDRVPDLKDLDSIGKAALVKATAINVPMCQKFTDLFEKMVPMAVQQAVSTYSQRKSEIVNRLIGQMREATNLCNGVLASLNLPAALEDLSGDSVPQSILEKSRAIIQQGGLQSIDRLIKDLPELLQRNREILDESLKILDEEEATDTELRTKFSQRWQRTPSNDLYKPLRTEGSNFRNVLDKAVQADKVVRERYHTHCDMIALLCKPEPELNAAIPSANPAKTLQGSEVVGVLRSQLSKLDEVKREREQLENDVKSVQFDMTSKFLTALAQDGALNEEAISVTELDNIYGGYTQKVQQSLKTQEEILGNIQTSHQEFSAMKQSNTDAKEREEVLKKLASAHDSYVEISNNLKEGTKFYNDLTEILLKFQNKCSDIVFARKTERDELLKELQQSIAREPSAPSFPTVPAYQCNPSTPTVTASGPPGAPTPAPRTVFQSKPQPPARPPPPVICSAATSTSTPLPTTVVASTDAPSVSTTLSMVPPGAPSQAQGPPYPTYPGYQGYMQMPMAYNPYAFGQYNIPYMYQGPGQGSFPGAQPPQQHPYPYPQQPQQPYYPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCZ5</accession>
    <name>W5MCZ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inorganic diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01025166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCZ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006254"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006262.1">
        <property type="protein sequence ID" value="ENSLOCP00000006254.1"/>
        <property type="gene ID" value="ENSLOCG00000005179.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017004"/>
    <dbReference type="InParanoid" id="W5MCZ5"/>
    <dbReference type="OMA" id="CASQYNA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005179">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
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        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="294" mass="33091" checksum="D92D7FF6BA943133" modified="2014-04-16" version="1">MSFTIEERGNPNTLSYRVFLKDSKGKYISPFHDIPIYANETQNIFHMVVEVPRWTNAKMEIATKDALNPIKQDVKKGKLRYVANVFPHKGYIWNYGAIPQTWEDPGHKDDDTGCCGDNDPIDVCEIGSKVCSRGEVIKVKVLGTLALIDEGETDWKVIAINIDDPEAKELNDIDDVRRLKPGYLEATVDWFRKYKVPDGKPENQFAFNGEFKDKDFAIEIIKSTHGFWKALVSKKTSSGELSCTNTCISDSPFCCSEAEAQAVIDATCPCGNPEPLPSTVENKEMQTHMKYCGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD39</accession>
    <name>W5MD39_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 48 member 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRG family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629309.1">
        <property type="nucleotide sequence ID" value="XM_006629246.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD39"/>
    <dbReference type="FunCoup" id="W5MD39">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000006306.1">
        <property type="protein sequence ID" value="ENSLOCP00000006298.1"/>
        <property type="gene ID" value="ENSLOCG00000005231.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691534"/>
    <dbReference type="KEGG" id="loc:102691534"/>
    <dbReference type="eggNOG" id="ENOG502S0AI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002307"/>
    <dbReference type="HOGENOM" id="CLU_148774_0_0_1"/>
    <dbReference type="InParanoid" id="W5MD39"/>
    <dbReference type="OMA" id="VWNIVYK"/>
    <dbReference type="OrthoDB" id="5954402at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005231">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026218">
        <property type="entry name" value="HRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31525">
        <property type="entry name" value="HEME TRANSPORTER HRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31525:SF1">
        <property type="entry name" value="HEME TRANSPORTER HRG1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02095">
        <property type="entry name" value="TRNSPORTRHRG"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="144" mass="16517" checksum="DE761F565F82AAF3" modified="2014-04-16" version="1">MGINKTYISIGYATFGVLMGFSAFFAWNIAFKQPWTAAMGGLSGVLALWALITHVMYVQDYWRTWLKGLKFFLFIGGLFALLAVVAFIAFLSLAITRHQSLTDPRSYYLSCVWSFLTFKWAFLLGLYSHRYRKEFADISILSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDX4</accession>
    <name>W5MDX4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="29">Solute carrier family 25 member 30</fullName>
        </submittedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R,3R)-tartrate(in) + sulfate(out) = (2R,3R)-tartrate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73403"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30924"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(3R)-citramalate(in) + sulfate(out) = (3R)-citramalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73227"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3S)-citramalate(in) + sulfate(out) = (3S)-citramalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73223"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30936"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(S)-malate(in) + sulfate(out) = (S)-malate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:71615"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(S)-malate(out) = (S)-malate(in)</text>
            <dbReference type="Rhea" id="RHEA:74555"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(S,S)-tartrate(in) + sulfate(out) = (S,S)-tartrate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73407"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30927"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-aspartate(in) + sulfate(out) = D-aspartate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73399"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73395"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>maleate(in) + sulfate(out) = maleate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73199"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>malonate(in) + sulfate(out) = malonate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73195"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sulfate(in) = sulfate(out)</text>
            <dbReference type="Rhea" id="RHEA:34983"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>sulfate(out) + phosphate(in) = sulfate(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:71631"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>sulfate(out) + succinate(in) = sulfate(in) + succinate(out)</text>
            <dbReference type="Rhea" id="RHEA:73411"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>sulfite(in) + sulfate(out) = sulfite(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73207"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>thiosulfate(in) + sulfate(out) = thiosulfate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73215"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 28">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639078.2">
        <property type="nucleotide sequence ID" value="XM_006639015.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDX4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006583"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006591.1">
        <property type="protein sequence ID" value="ENSLOCP00000006583.1"/>
        <property type="gene ID" value="ENSLOCG00000005454.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686086"/>
    <dbReference type="KEGG" id="loc:102686086"/>
    <dbReference type="eggNOG" id="KOG0753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158961"/>
    <dbReference type="InParanoid" id="W5MDX4"/>
    <dbReference type="OMA" id="IMPALNW"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005454">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000006">
        <property type="entry name" value="brain mitochondrial carrier protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 27 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="30"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="127"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="221"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="34968" checksum="5C5301DB15F2DCCC" modified="2014-04-16" version="1">MTCLQTVEPVEITGIREEAYAHTMSNLNWKPFVYGGLASVTAECGTFPIDLTKTRLQVQGQVSDSKYREIRYRGMLHAFVRICREEGLRALYSGIAPAMLRQASYGTIKIGTYQSFKRLFVDRPEDETLVMNMLCGVLSGVISSSIANPTDVLKIRMQAQGSVVQGGMIGNFINIYQQEGTRGLWKGVSLTAQRAAIVVGVELPVYDLTKKHLILSGYMGDTVYTHFVSSFVCGLAGALASNPVDVVRTRMMNQRVLLDGTCSGYKGTLDCLVQTSRSEGFFALYKGFFPNWLRLGPWNIIFFITYEQLKKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME06</accession>
    <name>W5ME06_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Brain-specific homeobox protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME06"/>
    <dbReference type="FunCoup" id="W5ME06">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006615"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006623.1">
        <property type="protein sequence ID" value="ENSLOCP00000006615.1"/>
        <property type="gene ID" value="ENSLOCG00000005483.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165707"/>
    <dbReference type="HOGENOM" id="CLU_104234_0_0_1"/>
    <dbReference type="InParanoid" id="W5ME06"/>
    <dbReference type="OMA" id="DLPGKHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005483">
        <property type="expression patterns" value="Expressed in brain"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021982">
        <property type="term" value="P:pineal gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019827">
        <property type="term" value="P:stem cell population maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000173">
        <property type="entry name" value="brain-specific homeobox protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF16">
        <property type="entry name" value="BRAIN-SPECIFIC HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="251" mass="28909" checksum="31644683A2DBD664" modified="2014-04-16" version="1">ISEPKHTQELTVYCMLGLDMNLNFTSPVHQMPAQRPTSFFIEDILLHKPKPLRDVFPSPFTSSLASRVPLLEYGYPLMPTPTILAPHPHHPLHKPDHHPYFLTTSGMQVPALFQHHPELPGKHCRRRKARTVFSDSQLSGLEKRFEIQRYLSTPERVELATALSLSETQVKTWFQNRRMKHKKQLRKTQDDQKSPNDLDQSLENNSSENEGNEKISLDVKTASAQNTYILEENEDDVDIEDDDICSPEHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEE2</accession>
    <name>W5MEE2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">F2R like trypsin receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219626.1">
        <property type="nucleotide sequence ID" value="XM_015364140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEE2"/>
    <dbReference type="FunCoup" id="W5MEE2">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006751"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006759.1">
        <property type="protein sequence ID" value="ENSLOCP00000006751.1"/>
        <property type="gene ID" value="ENSLOCG00000005593.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698065"/>
    <dbReference type="KEGG" id="loc:102698065"/>
    <dbReference type="CTD" id="100073342"/>
    <dbReference type="eggNOG" id="ENOG502QR8S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244840"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5MEE2"/>
    <dbReference type="OMA" id="SQSHVYA"/>
    <dbReference type="OrthoDB" id="9370401at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005593">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002281">
        <property type="entry name" value="Pro_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF106">
        <property type="entry name" value="COAGULATION FACTOR II (THROMBIN) RECEPTOR-LIKE 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01152">
        <property type="entry name" value="PROTEASEAR2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867688" evidence="13">
        <location>
            <begin position="22"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="72"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="128"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="377" mass="42955" checksum="FFBE32B37E3D460D" modified="2014-04-16" version="1">MFSVKFKVIFLLSSFISSYEAGSSRGFIGTSIDEKENVFKVNKIAEETLQSGLTTVFLPIVYIIVFVVSLPTNAMAIWVFLFRTKKKHPAAIYMANLALSDLLFVIWLPLKIAYHINGNNWIYGEGLCKVLVGFFYANMYCSILFITCLSVQRYWVIAHPLSTQRKNNRFAVCLSIAIWILIWLSTTPLYLYDQTVKIGNLDITTCHDVNKVGETNYPFGYFITMGIFAYFIPSLIIVAAYFLLLRTLSDSISDENTGKKRRKAFIIIITVLVTFLICFTPSNIMLVAHYSLLKQGFENNVYGFYIITLCLASLNSCLDPFIYYFVSKEFRDSVKNTLICRSARTVERMRVSFSSMKYSKRNDIYTSSSGSTNTSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEZ4</accession>
    <name>W5MEZ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylinositol 3-kinase regulatory subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">p55PIK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PIK3R3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3K p85 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015928">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEZ4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006953"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006961.1">
        <property type="protein sequence ID" value="ENSLOCP00000006953.1"/>
        <property type="gene ID" value="ENSLOCG00000005759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693436"/>
    <dbReference type="CTD" id="100000252"/>
    <dbReference type="eggNOG" id="KOG4637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156259"/>
    <dbReference type="HOGENOM" id="CLU_007031_1_0_1"/>
    <dbReference type="InParanoid" id="W5MEZ4"/>
    <dbReference type="OMA" id="LLCMAQE"/>
    <dbReference type="OrthoDB" id="3175255at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005759">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12925">
        <property type="entry name" value="iSH2_PIK3R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09930">
        <property type="entry name" value="SH2_cSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09942">
        <property type="entry name" value="SH2_nSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000075">
        <property type="entry name" value="phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000017">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit gamma b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000001">
        <property type="entry name" value="Putative phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PI3kinase_P85_nSH2"/>
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    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT GAMMA"/>
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        <property type="entry name" value="PI3KINASEP85"/>
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        <property type="entry name" value="SH2DOMAIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="17">
        <location>
            <begin position="120"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="343"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="636"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="452"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057933"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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            <dbReference type="ARBA" id="ARBA00082483"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="739" mass="84592" checksum="80EDD68A334E360B" modified="2014-04-16" version="1">MGSEVFHYRALFEYKKERGDDISLLPGDILAVYKTALLTPEYQEGDERSPNGWLNGINERTKEKGDFPGTFVEYVGPVRVGVPAPKPRPRPVPPTPGGTQTPGAMQTGSCLGQSDVAELLPLPDQASTVVMRLTEALERQGWESEPLYRAPLSPPRHPELKQALETDPSSVELDQYELPVLAEALKSYLQDLPSPFIPAGIYSELVYIAQETPNVEECGQQLKKILESPTIPQPNHLLLLHLTRHLYRVALCGGQSQATPRLLAQSFSEALFKHSLSSADVNPEHHVKIIEALIMAGGLAEMQAAPALPPKPPKQMTPANTNGTNVTSVIKDATSMSLQEAEWYWGDISREEVNDKLRDMPDGTFLVRDASTKMQGDYTLTLRKGGNNKLIKIYHRDGKYGFSDPLTFSSVVELISHYRHESLAQYNPKLDVKLMYPISRFQQDQLVKEDNIDAVGKKLQEYHNQYQEKSKEYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCHTQERYSKEYIERFRREGNDKEIERIMMNYEKLKSRLGEIHDSKIRLEQDLKKQALDNRETDKKMNSLKPDLIQLRKIRDQYLVWLNHKGVRQKRINDWLGIKNENLDDTYFVNEEDENLPHYDEKSWFVGDLNRTQAEDLLLGKPDGAFLIRESSKKGCYACSVVVDGEVKHCVIYSTPRGYGFAEPYNLYSTLKDLVLHYHQTSLVQHNDLLNVRLAYPVYAQMPSLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF17</accession>
    <name>W5MF17_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RILP-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Rab-interacting lysosomal-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RILPL family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF17"/>
    <dbReference type="FunCoup" id="W5MF17">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006976"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006984.1">
        <property type="protein sequence ID" value="ENSLOCP00000006976.1"/>
        <property type="gene ID" value="ENSLOCG00000005774.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692576"/>
    <dbReference type="CTD" id="353116"/>
    <dbReference type="eggNOG" id="ENOG502QR9G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157897"/>
    <dbReference type="HOGENOM" id="CLU_044133_3_0_1"/>
    <dbReference type="InParanoid" id="W5MF17"/>
    <dbReference type="OMA" id="SFGQWAD"/>
    <dbReference type="OrthoDB" id="10069524at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005774">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14445">
        <property type="entry name" value="RILP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1770:FF:000002">
        <property type="entry name" value="RILP-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051241">
        <property type="entry name" value="DZIP_RILPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021563">
        <property type="entry name" value="RILP_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502:SF6">
        <property type="entry name" value="RILP-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502">
        <property type="entry name" value="ZINC FINGER PROTEIN DZIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11461">
        <property type="entry name" value="RILP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161256">
        <property type="entry name" value="RILP dimerisation region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RH1" evidence="15">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="16">
        <location>
            <begin position="281"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="243"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="315"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="45415" checksum="BF75D284E33BE203" modified="2014-04-16" version="1">MEDFGSALEKNAAELTVMDVYDIAAVVGQEFERIIDQYGCEALARLMPKVVRVLEILEVLVSRNSISPETEELRLELDRLRLERMDRLEKEKKHQKELELVEDVWRGEAQDLLSQIAQLQEENKNLLTNLSFKDTGMTEEDLQKQEGMSERERQVMKKLKEVVDKQRDEIRAKDRELTLKNEDIEALQQQQNRLMKINHDLRHRITVVEAQGKALIEQKVELEAFAQTREQELGSLRLELSKLREKGQGDPSQGSEEPKEPVYEEDLSTDKAGLDLQDPNRPRFTLQELRDVLHERNELKAKVFMLQEELAYYKSEENGEETSSSAPWPAPPQPPRPATQPESGIKRLFSFFSKDKKRNSQKNVRFEDTFGGWANKDDVYTEQAQEALQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF89</accession>
    <name>W5MF89_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Pleiomorphic adenoma gene-like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF89"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007048"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007056.1">
        <property type="protein sequence ID" value="ENSLOCP00000007048.1"/>
        <property type="gene ID" value="ENSLOCG00000005839.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164771"/>
    <dbReference type="HOGENOM" id="CLU_002678_66_1_1"/>
    <dbReference type="InParanoid" id="W5MF89"/>
    <dbReference type="OMA" id="DMMGGKS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005839">
        <property type="expression patterns" value="Expressed in embryo and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000231">
        <property type="entry name" value="PLAG1 like zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000256">
        <property type="entry name" value="PLAG1 like zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF31">
        <property type="entry name" value="ZINC FINGER PROTEIN PLAGL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="358"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="483"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="544" mass="60268" checksum="0DC6D6A605BECC45" modified="2014-04-16" version="1">HRSLEAWGAESAFSWPRPLKTAMAAAATDDPGQITAVTPEDEDECAAAAAKLFGGKPPRSERAERDDRNPRSCECEVCGALFSTQDKLRIHTYCHTGEKPFRCSQPQCAKAFVSKYKLFRHMATHSPQKTHQCTFCEKMFHRKDHLKNHLQTHDPNKEAFKCEECGKNYNTKLGYKRHVAMHAATSGDLTCKVCLQSYESTPVLLEHLKSHSGKSSGGAKEKKHPCDHCDRRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHVKKSHSQELLKIKSEPPDMLGLLNSGSPISVKEEFSPMMCMASSKDSMMSKSFSNNFPMGMYNPHLQSMSNSGVTHSLMPNSLSAAMGMGCPMESPSPIHHHSPPPQQPPPPKYQLGSTSYLLEKPLKVEMESFLMDLQSGLPVSCAESHPSPPKDGLEHQPSLDDLSGEHLLSKSPAIIAESLCAANMDFSHLLGFLPLNLPPYNPPMSSSGLVMGYSSQGDPQPPLTSLQPQPQETQGSVSSLSLGPLHPLPPVFTSSLSTTTLPRFHQAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFK4</accession>
    <name>W5MFK4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Centrosomal protein of 104 kDa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Required for ciliogenesis and for structural integrity at the ciliary tip.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with CCP110 and CEP97. Interacts with ARMC9, TOGARAM1, CCDC66 and CSPP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFK4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007163"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007171.1">
        <property type="protein sequence ID" value="ENSLOCP00000007163.1"/>
        <property type="gene ID" value="ENSLOCG00000005920.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013405"/>
    <dbReference type="HOGENOM" id="CLU_003200_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFK4"/>
    <dbReference type="OMA" id="VQGNDYN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005920">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000200">
        <property type="entry name" value="Centrosomal protein of 104 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052607">
        <property type="entry name" value="CEP104-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048739">
        <property type="entry name" value="CEP104_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048738">
        <property type="entry name" value="CEP104_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371:SF0">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 104 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371">
        <property type="entry name" value="GLYCINE-, GLUTAMATE-, THIENYLCYCLOHEXYLPIPERIDINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21038">
        <property type="entry name" value="CEP104_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21039">
        <property type="entry name" value="CEP104_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="UVR" evidence="14">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="306"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="890"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="682"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="939" mass="105935" checksum="03FB96277EF8DF31" modified="2014-04-16" version="1">MPHKIGFVVVSSSGHEDNYNAKELMVHAPTVNGWRSARFCPYPQEITLQLVERCRIRKLQLLAHQYMISAKIEFYISDSLPESFSPNPSDRFCRLGYVSLSDNEKTGFKARELKSVHVDAVGTYLKLIFHKNHINRYNLYNQVALVAINVLGDSVDSSEMDVIPTRDQLIEHYLNSIHNDVTLEGTYLSKSDSISPLDDLAFDMYQDPEVAQIIRQLDEKKHEAVRQERFDLAKKLKQAIADLQKVGEHLGRYEVEKRCAIDKEDYDLAKQKKQQMDEYRLKVYQQLEIHNLLDMGSIRKMADLPLEPLSHPASPQQVKPAEIPPQKKREKKPEKPPDPEPSLPARPMTPLQTPSPPSTVARPEPPKISVSALPYDERPLPALRKQTGERQSQLEESPPPDSSRTPRTPGITGEPEPLTEKALREATFAIEVYGEGLVAGAYSKTWSYREDALVAVYKKLMDVPSGMPKEELKTLLRAAIFLVRKSLSDKVSSVFQASLKLLKMVITQFIPKHKLGRVEAAHCLEQTWPNLILRTGDSATRLRVVATNFIQEMALFKEVRPLQVIPAELVKPMKSNTPSRLALSQVELLEKLLKELGTDGSGFTINNVMRFAIGALEHSTSEVRETASRIIQDMYRQHKSAITDYLPPDDASTRRNVLYKNIFDGFAKIDGRPAETQTKTLKKAARQEAEKEKKEEIRALQEQLAALKDLTTNGKKDKNKVKDKEAHKPTKAGVYRFRKVPPAHPPDIADDDSSFANYLDNLCIFCGEKDDSFTEEGLDLHYWKHCPMLSRCDLCRQVVEIASMSEHLLSECESKSNLTKCQRCAEAVPKDELSEHVRSKACNPPKSEKLSNHCSLCHENFTPGDEAWKAHLMGRDGCKQNSRRVPALQRTQQIQGKMGPSATKQAPLGMRGRSMATSSKIPAPRAGVNKMGGRNPVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG05</accession>
    <name>W5MG05_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01028878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG05"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007314"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007322.1">
        <property type="protein sequence ID" value="ENSLOCP00000007314.1"/>
        <property type="gene ID" value="ENSLOCG00000006058.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063836"/>
    <dbReference type="HOGENOM" id="CLU_019830_0_0_1"/>
    <dbReference type="InParanoid" id="W5MG05"/>
    <dbReference type="OMA" id="RMMSGNR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006058">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16812">
        <property type="entry name" value="RING_CH-C4HC3_MARCH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052297">
        <property type="entry name" value="RING-CH-type_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471">
        <property type="entry name" value="MARCH7/10 E3 UBIQUITIN PROTEIN LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="10">
        <location>
            <begin position="586"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="343"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="721"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="743"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="757" mass="83181" checksum="CE5B8784243D44A2" modified="2014-04-16" version="1">MDSKPRRLPFMQSSPGPSSSLSSSSSLSSASSPLGSSRLYSNRGSVLSTERYTRGTSVKVESEYQGSRYHSTPRDYSLPESRHSSWKLCSPSLTPPSVSCDRSWSVETRSKLGESERRQGTYCGLLNASQDGDSKRPKLSYSNRATYSRSVSSSGANSTGIGSISDSSWKYSPVSRLSSSSTDSTRPRRELDKRAEPSLSNLGEHSRRGGVLSSSAYLPDSATSSYAQGARPKESSFSSTRMSSSVNHYMPSEYQPSLLSRDSYRNTSRSATSTTLRAQESSQASGGLDSRNMSSRSPNWWYNPPAERVTSSSPRRSNETSDSEGRRTTRQLLSRLAFSMSSTLFSRRSSQDSSGSSSRSFESSSEDPFDVPRDENSSQSSDSRNSSPDVSERRAPILETRNSQADPESSRSAATESRSSGSWLSSSFRNRCTPLFSRRRREGRDETARMASTSEEPYGNSQYSWTRKESPEPKASAEEPEEESQGAGATASSVAAAAAAAASSASVQGTSQDERNPRISEIVPNSLFRLAMPSTLESSLSDNVMITVDIMAAGRPQPDGQENDKAAPSRDPEKLKKIQESLLLEESDDEGDLCRICQMGADSSSNPLIEPCKCTGSLQYVHQECMKKWLRSKISSEGSSLEAITTCELCKEKLHLNIENFDINELYRTHASERAEYEFISCGLYLVVLLHLCEQRFSDVLGAANDAGFFNLARTLHEHMDDLESSYEESEEEAETVSRPSFDFEDDDEEQEEDSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGM5</accession>
    <name>W5MGM5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cyclin-dependent kinase</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Galactosyltransferase-associated protein kinase p58/GTA</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01009815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGM5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007534"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007542.1">
        <property type="protein sequence ID" value="ENSLOCP00000007534.1"/>
        <property type="gene ID" value="ENSLOCG00000006226.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158459"/>
    <dbReference type="InParanoid" id="W5MGM5"/>
    <dbReference type="OMA" id="YYNVMLG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006226">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07843">
        <property type="entry name" value="STKc_CDC2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000054">
        <property type="entry name" value="Cyclin-dependent kinase 11B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000124">
        <property type="entry name" value="cyclin-dependent kinase 11B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045267">
        <property type="entry name" value="CDK11/PITSLRE_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF107">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="443"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="739"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="126"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="800" mass="93543" checksum="E34F5C0459A90F22" modified="2014-04-16" version="1">MGDEKDTWKVKTLDEILQEKKRRKEQEEKSDGKRQKNADERDSKRDSLEEGELRDHRMEITIRNSPYRREDSTEDRGEEDESLAIKPPQQVARKEKSHHRKEEKRKDKRRHRSHSADGAGKHMRVKEKEKERENERRKRQQREEQDKARREWERQKRREMARAHSRRERDRLEQLERQRERERKMREQQQKEQREQKERERRAEERRKEREVRREARAVASHHRGVKEEYGEKPKQSHRSRSPARPQRDRLEQAETRRIAVREQRQENRDLLSDLQDVSDSERKTSTAESSSGRHSSALDREKNPLFVSSGTGSEEEEEEETSSETEVEEEESGTNTEGSEQSADEVSEEEQSEEEYEEERENGNHVPAVPESRFDHDTEESVEEEEEEEVEEAEPSPHSHSHSSTPEENYIPDSPPMSPVELKKELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVRHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPLKPYTPVVVTLWYRAPELLLGAKEYSTTIDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKDLGTPSEKIWPGYNELPAVKKMSFTEYPYNNLRKRFGALLSDQGFDLMNKFLTYCPSKRITAEEALKHEYFRETPLPIEPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKDTGFHLTTTNQGASAAGPGFSLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHF0</accession>
    <name>W5MHF0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHF0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007809"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007819.1">
        <property type="protein sequence ID" value="ENSLOCP00000007809.1"/>
        <property type="gene ID" value="ENSLOCG00000006462.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685745"/>
    <dbReference type="CTD" id="114876"/>
    <dbReference type="eggNOG" id="KOG2209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155295"/>
    <dbReference type="HOGENOM" id="CLU_007105_5_1_1"/>
    <dbReference type="InParanoid" id="W5MHF0"/>
    <dbReference type="OMA" id="HAESPHF"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006462">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13285">
        <property type="entry name" value="PH_ORP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000094">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000686">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000231">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000005">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF53">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="237"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="510"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="795"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="883"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="961" mass="108511" checksum="38E66F06872856AB" modified="2014-04-16" version="1">MEVNPEDQFLHDARNGNHEGIRKVLESKKQGETDMNINCKGKSKSNLGWTPLHLACYFGHKDVVEELLKAGADVDVLNNMGDTPLHRAAFTGRKEVVMLLLQYNACATVLNGTAQTPRDVTKDVEIRNMLEAAERTEERKQEELLLEAAREGNSTTLTELLNRKKPPDINCSDLLGNTPLHCAVYRGQKQCAIKLLKSGASPNIKNQNDQTAFDLVNSAEMKQILEGNYLKGLNRNVPSFEGPLWKSSRFFGWRSYWVVLQDGVLSWYAKQSDAASNNRRQGCKSLTQAHCTVKAKDNSHFSIKCFDDSIHLFKVHPKNEVTIKKWLDTIEEHAAYSTHYCSQEQGSEEDEEDGVSVRELSDSLQKAQVCQQKLETAVSNFLAMVKNDKVAQGLLPPVLQNVKELSELSSETCSALRDCLALFSKQEGVQNLRLEQEQEKTKILSEALQTLATEHHELEQSVAKGSPPRTALSEDEFYDAVSDSDSECSLSGFETVASYSFDEETIQFSSNRSSPVSMSEENSPDNQGPQSNGIKKHRTSLPAPMFSRNDFSIWSILRKCIGMELSKITMPVIFNEPLSFLQRLTEYMEHTYLIHKANASPDSIERMKSVAAFAVSAVASQWERTGKPFNPLLGETYELVREDLGFRLISEQVSHHPPVSAFHAEGLKNDFNFHGSIYPKLKFWGKSVEAEPKGIITLELPKYNEAYTWSNPTCCVHNIIVGQLWIEQYGNVEITNHKTGEKCCLTFKACGLFGKELHKVEGYILDKSKKKLCALYGKWTECLYTVDAATFDAHKKTDKKSSEDKKSSKPSSSAEDPDEMPRPDAESVQVIPGSELLWRIAPRPPNSAQMYNFTTFAMQLNELDKEMEGVIPKTDCRLRPDIRAMENGDIDLASEEKKRLEEKQRAARKNRSKSDDEWKTRIPALGPRWFHQGPNPYNSSADWLYSSGYWDRNYSQLPDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHI3</accession>
    <name>W5MHI3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Development and differentiation-enhancing factor-like 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Promotes cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHI3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007842"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007852.1">
        <property type="protein sequence ID" value="ENSLOCP00000007842.1"/>
        <property type="gene ID" value="ENSLOCG00000006485.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165482"/>
    <dbReference type="HOGENOM" id="CLU_006942_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHI3"/>
    <dbReference type="OMA" id="KNTHCEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006485">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08834">
        <property type="entry name" value="ArfGap_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07604">
        <property type="entry name" value="BAR_ASAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11821">
        <property type="entry name" value="SH3_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000036">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035836">
        <property type="entry name" value="ASAP1-like_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF5">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="21">
        <location>
            <begin position="304"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="22">
        <location>
            <begin position="419"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="616"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="948"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="741"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="878"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="764"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="881"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112692" checksum="5B288A93ADF5CA0F" modified="2014-04-16" version="1">MPEFISVDEFIAETLEDYSSPTTSAFTTKMSSCRNAVNSIEEALDSDRTVLQKMKKGAKAKHASGQDHILHLESYINSMEKLAVNFRNNGEQELASAFNKFAEYSKELLTPMKNALQSMYHNINFSLESLVKGDLKEVKGDLKKPFDRAWKDYENKFTKIEKEKRELAKQYGMVRTEVSGGEIAEELEKERRMFQLQMCEYLIKVNEIKTKKGFDLLQNLIKHYHSQCNFFQECLAATEKLKQYIEALTTGLNSIKHRQDEEKRQLCSLRDQLRLSLQPEQKEDSLSRQAVYSMHQLQGNKQYGTEKTGCLYKKSDGLRKVWQKRKCAVQNCYLTIAHGTPNRPPAKLNLLTCQVKPSQEDKKCFDLISHNRTYHFLADDEAECVAWISVLTNSKQEALNVALDERRPSGENSIEDLTKSIIEDVRRMPGNNVCCDCRAPDPTWLSTNLGILACIECSGIHREMGVHYSRIQSLSLDKLGTSELLLAKNVGNAGFNEIVEANLPSSSLKPSADSDMAVRKDFILSKYMERQFAQHSGGSPGTLRRSLQEAVRSRDIFALLRLYGERLDLSQPLHSPVQEPGEAALHLAVLLADRTSLHMVDFLVHNCNNLDKQTVRGNTALHYCCLHNKTECLKLLLRAKANTHIKNEAGETGLDIARRLKHSHCEELVRQTSFQQIGIGQLCAELNWKRHLAKAHRAAVPCPAQLGPVKKERSCRPFSLYQASSPAGPDRGGHASVGRRLASLVRDPRRETYASPSTQTRSLDPPPPPPSSPAPPLPPRIRAPNVPPPPPPPASPPDARCDSVPGRRKRTALPSLAACEWVFFEPAPVVLPSPSAFRFSFPDYGFPGNSRGYSGPEGVYQKSALGSPKYTVTTLNALPELPEKGTGRADGEGSSPQCIYATPQPSGRARLPSSPPFRADGPEPNGASPALLGPPAPHPLPRKALAKPRVRRVKAIYDCVADHHDELTFSEGEVLVVMAEEDADWWLGYIEGHPHKRGLFPASFVHILSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHM5</accession>
    <name>W5MHM5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="4 14">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="function">
        <text evidence="14">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="4 14"/>
    <dbReference type="EMBL" id="AHAT01020936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHM5"/>
    <dbReference type="FunCoup" id="W5MHM5">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007884"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007894.1">
        <property type="protein sequence ID" value="ENSLOCP00000007884.1"/>
        <property type="gene ID" value="ENSLOCG00000006511.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="InParanoid" id="W5MHM5"/>
    <dbReference type="OMA" id="DVGDIMV"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006511">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF26">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="16">
        <location>
            <begin position="29"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="32767" checksum="F31B2CF980EE78AD" modified="2014-04-16" version="1">SNQISFSYTFEDYKETADWLLTRTDLRPKVAIICGSGLGGLADLLGDKAIFPYQDIPRFSQSTVPGHSGQLVFGKIEGKPCVCMQGRFHFYEGYALTKVTFPVRVFHLLGVKTLIVTNAAGGLNPDFQVGDIMFIKDHLNIPGITGQNPLFGPNEERFGVRFPCMSDAYDKGLLRIANKTASELGFSDFVRSGVYCMAAGPSYETVAECRLLRTLGGDAVGMSTVPEVVVARHCGLQVFGISLITNKVVMDYDSQEKTNHEEVLQTSKMRAKDLQSLVCSFLGKIEDQFQEPEKNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI01</accession>
    <name>W5MI01_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complex I assembly factor TIMMDC1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Translocase of inner mitochondrial membrane domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Participates in constructing the membrane arm of complex I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI01"/>
    <dbReference type="FunCoup" id="W5MI01">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008010"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008020.1">
        <property type="protein sequence ID" value="ENSLOCP00000008010.1"/>
        <property type="gene ID" value="ENSLOCG00000006630.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013817"/>
    <dbReference type="HOGENOM" id="CLU_068982_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI01"/>
    <dbReference type="OMA" id="SYMNFME"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006630">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055299">
        <property type="entry name" value="TIMMDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13002">
        <property type="entry name" value="C3ORF1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13002:SF1">
        <property type="entry name" value="COMPLEX I ASSEMBLY FACTOR TIMMDC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="291" mass="32237" checksum="8355575A2FEBF9EF" modified="2014-04-16" version="1">MSACPRLDKPPRGSSYSTLFWGLVPFPRVLAADGVGSGAPAENAVQLPRYISKPELPDTGWDRIKELFDRSEMQAYPEEILNIVKSGLTAAVVGMVYGGIPAARQAREHFIQQSQAEVYRHQVEAVRSAHNAAIRGFIRFGWRWSWRVAAFVTLFSTVSTGLSVYRDKNSLSHYSAAGAITGGLFRLNMGLSGMLACSAIGAVLGVPAGALMMAVQRLTGETHRERKRRERRELYELKLAEWNARLNVTEGLIDEMSKVEDGSIETDLDRIEELLNLPRNEEASASKQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ55</accession>
    <name>W5MJ55_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ55"/>
    <dbReference type="FunCoup" id="W5MJ55">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008414"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008424.1">
        <property type="protein sequence ID" value="ENSLOCP00000008414.1"/>
        <property type="gene ID" value="ENSLOCG00000006960.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157768"/>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_1"/>
    <dbReference type="InParanoid" id="W5MJ55"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006960">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000015">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="20">
        <location>
            <begin position="43"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="18">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="49173" checksum="1470F9111E4F5FF5" modified="2014-04-16" version="1">KMLNLPKLLKKSTKCNLFRLRNCIYRASPRLRYYGTAYPPETRRRVVVTGIGLVSPLGIGSSLPWERLIRAESGIISLDSEAYKNVPCKVAACVPRGEGEGQFKEEMFVSKGEMNSMSLATVMALGATELAMKDSGWYPLSTQEQFTTGVAVGMGMVPLDEIARTAVLFETKGYGKVSPFFVPRILVNMAAGHISIKYKMKGPNHAVSTACTTGAHAIGDAARFICHGDAAVMVAGGTEACIGPLSISGFARARALSTSWNAAPKLASRPFHPEREGFVMGEGAAVLVLEEYNHAIDRGATLYAEVLGYGLSGDASHITAPTTDGDGAFRCMSAAIKDAGIKPEDVTYVNAHATSTPLGDAAENTAIKRLFQKHAYSMAVSSTKGATGHLLGAAGALEAAITVLACHHGVLPPTLNLDRTDPEFDLNYVPCAAQEWKTEGRRIALSNSFGFGGTNATLCFASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJF1</accession>
    <name>W5MJF1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD synthase</fullName>
            <ecNumber evidence="7">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01019901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MJF1">
        <property type="interactions" value="580"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008510"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008520.1">
        <property type="protein sequence ID" value="ENSLOCP00000008510.1"/>
        <property type="gene ID" value="ENSLOCG00000007025.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007266"/>
    <dbReference type="InParanoid" id="W5MJF1"/>
    <dbReference type="OMA" id="NSHFLCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007025">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000113">
        <property type="entry name" value="FAD synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="21">
        <location>
            <begin position="2"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="467" mass="52069" checksum="4620779C4EF936B8" modified="2014-04-16" version="1">XTQDTNSWFLCRGLRASGVSVRRVAVVPDDVGEIAAEIAAMAPRHTFLLTSGGIGPTHDDVTFAAVAQAFGEPLHPHPELARLVQGFFGVSEPGSAPMKLALVPASARLHYGADPATGRQLRFPLVSAHNVYIFPGVPSLLEAAFEGLRHLFASPGTRFHLRELFVDADEAALAPALARAQARFGRRVGLGSYPDWLSNYHRVRLTLDSDSAESVEEARRHLLDALPPGCVVPLVAEPVAVATQEVYRLAQSGTALGLKVASAVQTIETALEQYPPSQICVGFNGGKDCTALLHLYYAVLRRKFPQAEDRVKALYIRIVSPFPEMERFLKDTIKRYDLEVYMVEGNIRQALIDVQEQHPELQAVLMGTRRTDPYSRTLTPLCPTDPGWPHYMRVNPLLDWSYHDIWEFLRTLYVPYCILYDKGYTSLGSMDNTTRNPALQFVDERGVVRHRPAYQLENEEDERNSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJG0</accession>
    <name>W5MJG0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large neutral amino acids transporter small subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-type amino acid transporter 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 43 member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-isoleucine(in) = L-isoleucine(out)</text>
            <dbReference type="Rhea" id="RHEA:70943"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-leucine(in) = L-leucine(out)</text>
            <dbReference type="Rhea" id="RHEA:73011"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-methionine(in) = L-methionine(out)</text>
            <dbReference type="Rhea" id="RHEA:70939"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-phenylalanine(in) = L-phenylalanine(out)</text>
            <dbReference type="Rhea" id="RHEA:27950"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJG0"/>
    <dbReference type="FunCoup" id="W5MJG0">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008519"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008529.1">
        <property type="protein sequence ID" value="ENSLOCP00000008519.1"/>
        <property type="gene ID" value="ENSLOCG00000007037.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTQJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153576"/>
    <dbReference type="HOGENOM" id="CLU_035676_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJG0"/>
    <dbReference type="OMA" id="CTHTIIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007037">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015804">
        <property type="term" value="P:neutral amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766:SF2">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="575" mass="63848" checksum="C17188B98C74A229" modified="2014-04-16" version="1">MAPTLATAHSRRWWMAVTAVIENLLFSAVLLGWGSLLIMLKSEGFYSYLCPEPENRTNSSLGANHTEEEMVRMNGWLTCKDQDEMLNLAFTVGSFLLSAITLPLGIVMDKYGPRKLRLLGSACFAISCLLIAYGANNPNALSFLIFIALALNGFGGMCMTFTSLTLPNMFGDLRSTFIALMIGSYASSAVTFPGVKVIYDLGVTFILILLVWAACAGLVFINCFFNWPLEPFPGPEDMDYTVKIKFSWLGFDHKITGKQFYKQVTTVGRRLSVGNSMKQNPKELAAQDGNKLCLSTVDLEVKCQPQTLVLPTFLYQGSMWHQSQLLSLMTKHLCRTALTAIWHLKSESWVMSFLSSSSVHSDSISVSLYTTIFGVLQLLCLLTAPVIGYIMDWKLKECEDSDSETQEKEPSKDQNPPKRRDRQIQKITNATRAFIFTNLLLVGFGVTCLIPDLHLQIISFILHTIVRGFIHSAVGGLYAAVYPSTQFGSLTGMQSLISALFALLQQPLFTAMMGPLNGDPLWVNIGLLGLSMLGFCLPLYLLCFRRQLVRKRKQKAEDAKIFLKINGSGAHEAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKH1</accession>
    <name>W5MKH1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKH1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008880"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008891.1">
        <property type="protein sequence ID" value="ENSLOCP00000008880.1"/>
        <property type="gene ID" value="ENSLOCG00000007331.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="HOGENOM" id="CLU_049894_7_0_1"/>
    <dbReference type="InParanoid" id="W5MKH1"/>
    <dbReference type="OMA" id="NDNKCEL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007331">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="224"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="352" mass="39088" checksum="9A7D05E0583381C4" modified="2014-04-16" version="1">MSGDVLYSNVTFCANGSATTAVKQDEVTYAEVKTARAPSAQTAPPPGAPGKQDEVTYENVGTAGSAPAQTALSPEQPVKAPSIPLPYRLAAVCLGLLCAVLLIAIIVLIFFYNGNSQNISKLVKELDHLRVNYSNLTAAHNGLEVDFRNLTATHNKLQEDFRNLTVSYSNLTGINDKLQNKTEILQSLISKLKKHFSVDQYCPVTDQNTLEKDCRPCPWGWELFSTKCYYFSTDALSWNYSRTACRKLGADLVVINNRTEQEFIQKQIKGGEYWMGLSDAAAEGTWIWVDGTQPIECFRYWMKNQPDDYENEDCLATATPTADQITSLNNWNDNKCELPLLRICEGSSLPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKN3</accession>
    <name>W5MKN3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rho-related GTP-binding protein Rho6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Rho family GTPase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Rnd1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01032428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629480.1">
        <property type="nucleotide sequence ID" value="XM_006629417.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKN3"/>
    <dbReference type="FunCoup" id="W5MKN3">
        <property type="interactions" value="665"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008942"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008953.1">
        <property type="protein sequence ID" value="ENSLOCP00000008942.1"/>
        <property type="gene ID" value="ENSLOCG00000007372.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693005"/>
    <dbReference type="KEGG" id="loc:102693005"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158666"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_1_1"/>
    <dbReference type="InParanoid" id="W5MKN3"/>
    <dbReference type="OMA" id="PIVARCK"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007372">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000569">
        <property type="entry name" value="Rho-related GTP-binding protein Rho6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="232" mass="26024" checksum="795954D60BAFF396" modified="2014-04-16" version="1">MKERRNAQPVVVRCKLVLVGDVQCGKTAMLQVIAKDCYPETYVPTVFENYTACLELEEQRVELSLWDTSGSPYYDNVRPLCYSDSDAVLLCFDVSRPDTIDSALKKWKTEILDFCPSTRILLIGCKTDLRTDVCTLMELSNQKQVPISHEQGSAMAKQLGAEAYLECSAFTSEKSIHSVFRTAALACMNRLQPPAEPSPARRLSKRLLHLPSKSELLTSTFKKEKAKSCSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNL0</accession>
    <name>W5MNL0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination factor 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mTERF domain-containing protein 1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNL0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009981.1">
        <property type="protein sequence ID" value="ENSLOCP00000009969.1"/>
        <property type="gene ID" value="ENSLOCG00000008210.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005801"/>
    <dbReference type="HOGENOM" id="CLU_042536_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNL0"/>
    <dbReference type="OMA" id="NPFWLMF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008210">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903108">
        <property type="term" value="P:regulation of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000002">
        <property type="entry name" value="transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF194">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="35174" checksum="A94B66937376EF98" modified="2014-04-16" version="1">PRLELEAAPPLSPFAELSEDDAVQISVHPVLPAVSVTLRDYVDQSETLSKLVLLGVDLSKLEQRPNVANMLLRLDFDADVKERLLFLKEVGVEETKLGPFLTKNPFILTESMEKLQARVSYLKVKKFSSEAIARMVSEAPFLLNFSVERLDNRLGFFQKQLGLNVQKTRDLVTRLPRLLCGSLEPVKENLKVCKIELGFQENEIQHIVTRVPKLLTANKKKLTGIFDYLHNTMGIPHSLIVKFPQVFNAKLLRIKERHLFLKQLGRAQYDPAEPNYVSLDRLVSLPDEEFCTAVAMASLEDFEMFQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP65</accession>
    <name>W5MP65_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637275.1">
        <property type="nucleotide sequence ID" value="XM_006637212.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP65"/>
    <dbReference type="FunCoup" id="W5MP65">
        <property type="interactions" value="1479"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010174"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010186.1">
        <property type="protein sequence ID" value="ENSLOCP00000010174.1"/>
        <property type="gene ID" value="ENSLOCG00000008372.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696275"/>
    <dbReference type="KEGG" id="loc:102696275"/>
    <dbReference type="CTD" id="325464"/>
    <dbReference type="eggNOG" id="KOG3106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004010"/>
    <dbReference type="HOGENOM" id="CLU_057784_0_0_1"/>
    <dbReference type="InParanoid" id="W5MP65"/>
    <dbReference type="OMA" id="WKSRSCE"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008372">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="24410" checksum="C1456CC788877A31" modified="2014-04-16" version="1">MNVFRLTGDLSHLAAIIILLLKIWKTRSCAGISGKSQILFALVFTTRYLDLFTSFISLYNTSMKVIYIGCAYATVYLIYVKFKATYDGNHDTFRVEFLVVPVGGLSFLVNHDFSPLEILWTFSIYLESVAILPQLFMISKTGEAETITTHYLFFLGLYRALYLINWIWRFYFEGFFDMIAIVAGVVQTILYCDFFYLYVTKVLKGKKLSLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ08</accession>
    <name>W5MQ08_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TRAF family member-associated NFKB activator</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ08"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010467"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010482.1">
        <property type="protein sequence ID" value="ENSLOCP00000010467.1"/>
        <property type="gene ID" value="ENSLOCG00000008612.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRM3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008712"/>
    <dbReference type="HOGENOM" id="CLU_877050_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQ08"/>
    <dbReference type="OMA" id="KMKEHYE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008612">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039669">
        <property type="entry name" value="TANK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024581">
        <property type="entry name" value="TBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15249">
        <property type="entry name" value="TRAF FAMILY MEMBER-ASSOCIATED NF-KAPPA-B ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15249:SF0">
        <property type="entry name" value="TRAF FAMILY MEMBER-ASSOCIATED NF-KAPPA-B ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12845">
        <property type="entry name" value="TBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tbk1/Ikki binding" evidence="5">
        <location>
            <begin position="138"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="35721" checksum="76F496746B205CD7" modified="2014-04-16" version="1">MERGLDRNIGDQLNKAYEAYRQASIEKENARKELKQKTEAYDRYICELHQQLENQKQEIASLKSQLNSVGSLSKGRVKGSDATPEIKDSHLPAQRTHETKPLSQCDHLHETNRQRQKHVMESMKTPEMTVYSSPGISDDKSKEILEAFQEIQGKFHVIQNLTRKQKDHLNKLCRANDTAQEQQFSMPIQCTDVTVEQAEGPFTSAKKSDSDKETTSASITSRGVCPEEGEFMESLSKLSIKFPPAESEYDVFLNSAPERKLDLAPGRNDHALATSLPRVTDEQTVDIPVQYAFANPTSQTPSPSTSFTHENVRGPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQP1</accession>
    <name>W5MQP1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01003926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003927">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632191.1">
        <property type="nucleotide sequence ID" value="XM_006632128.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010700"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010715.1">
        <property type="protein sequence ID" value="ENSLOCP00000010700.1"/>
        <property type="gene ID" value="ENSLOCG00000008789.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694807"/>
    <dbReference type="KEGG" id="loc:102694807"/>
    <dbReference type="CTD" id="553646"/>
    <dbReference type="eggNOG" id="KOG1357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155786"/>
    <dbReference type="HOGENOM" id="CLU_015846_7_1_1"/>
    <dbReference type="InParanoid" id="W5MQP1"/>
    <dbReference type="OMA" id="CHRAING"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008789">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000004">
        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000047">
        <property type="entry name" value="serine palmitoyltransferase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF79">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="18">
        <location>
            <begin position="175"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="568" mass="63653" checksum="DC2EE1A8AED355E3" modified="2014-04-16" version="1">MTEGSVNKPANGEGCHRPANGVKPGRNGCVRGHYVYQQAPPPKHGYPEDKVHHIACNGGLYKRPFNESFEETPMLVAVLTYMGYGILTLFGYLRDFMREWKIEKCHIAREREEQKDFVPLYQDFENFYTRNLYMRIRDSWNRPICSVPGAKFDLVERVSHDYNWTFKYTGKVVKGVINMGSYNYLGFAENTGICADAAAEISKKYGAGVASTRQEMGNLDSHEKLEKLVARFLGVEAAMAFGMGFATNSMNIPALAGKGCLILSDELNHASLVLGARLSGSTIRVFKHNNMQSLEKLLRDAIVHGQPRTHRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPSGRGVVEYFGLDPTDVDIMMGTFTKSFGAAGGYIGGRKELIDYLRTHSHSAVYATSMSPPVVEQIITSMKCIMGEDGTTIGLERVQQLAENTRYFRRKLREMGFIIYGNDDSPVVPMMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCVSAAHTKEMLDTALNVISEVGDLLQLKYSRHRPVPLLDRPFDETTYEENED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR82</accession>
    <name>W5MR82_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin/FtsZ 2-layer sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR82"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010891"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010907.1">
        <property type="protein sequence ID" value="ENSLOCP00000010891.1"/>
        <property type="gene ID" value="ENSLOCG00000008940.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154457"/>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="InParanoid" id="W5MR82"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="49"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="226"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="41882" checksum="7B64B0940544F539" modified="2014-04-16" version="1">QRECISVHIGQAAVQFGNACWELYCLEHGVRPDETVFAVITADPLDSSFSTFSSKTGSSIHTVELEPTVIDKIRIGKYCQLQHLSSSVGKGGTHYASRKEMIDPLQDSVTSVITSCTGMVFWGFLVFHSFGGGPGAGFMSLLIGGKTSKLEFSVYPDPWSPLNTHITLEHSDSSFMVGSEAAFDICINVARPSYHNLNRLQFCKIYVQCPIKAQGGRNRLNYLRNQTLLVPYPRIHFPLVTNAHIISAEKAYDEQLSVPEIANVCVDHADQFIKCDGGRGKYMACCLLYCGDVVPKDVNAAIAAIQSRNSIQLVDWCPTGFQVGFTAVSGGDLAKLVQAVCMLSDRRGCLDHTFDLKGFCPLGDIEEGEFSAAREDMAALVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRF7</accession>
    <name>W5MRF7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01010148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRF7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010966"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010982.1">
        <property type="protein sequence ID" value="ENSLOCP00000010966.1"/>
        <property type="gene ID" value="ENSLOCG00000008988.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155265"/>
    <dbReference type="HOGENOM" id="CLU_014254_3_1_1"/>
    <dbReference type="InParanoid" id="W5MRF7"/>
    <dbReference type="OMA" id="HHIRICL"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008988">
        <property type="expression patterns" value="Expressed in mesonephros and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000129">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000345">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF469">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="102"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="492" mass="55049" checksum="7EA8F9FC0512B52A" modified="2014-04-16" version="1">FYSEEGEMDFKGKGLTLNEVNPYVRSIKVLDKEFIRMRAREIRKELLQGVKKPYKKLIDVSSGDAHMAGMKPISFLRQVLAVCLYPELLHSDRFPSDVEQRAQRLLKNCEGGSLGSYSPPCGIVSIRRSVSEFISQRDGGVPSDPDNIFITSGGQDAIRCILKILVHGEGHAQTGVLMPVPSYSYFKIAVLEVGGAIVPYHLSEEQGWDMQVNELRRALQTARGHCSPRALYIINPGNPTATGHVQSRESIEEVIRFAAEEKLFLLVDEVYQQTVFGEGSAFVSYKKVLYEMGPSYSDMVELASLHSVSKGFTGECGLRCGYVEFVNLDPEVKKCAIRLFEMYLSSSITGQIALDIMTNPPGHEDPSYPIFKAESQAILETLAHNARRVQETLDSLPGVSYQPVQGSLYIFPRLHLPPRAVEQARAEGMEPDRFYCSRLLEEAGVCSTPGCDVGQREGTYHIRLCNLVPLDTMEDMLDRLNSFHPRFMQEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRX7</accession>
    <name>W5MRX7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01010162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRX7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011136"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011152.1">
        <property type="protein sequence ID" value="ENSLOCP00000011136.1"/>
        <property type="gene ID" value="ENSLOCG00000009134.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154756"/>
    <dbReference type="HOGENOM" id="CLU_013555_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRX7"/>
    <dbReference type="OMA" id="MCEVPLI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009134">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000425">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000954">
        <property type="entry name" value="Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001084">
        <property type="entry name" value="obscurin-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000211">
        <property type="entry name" value="Obscurin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000502">
        <property type="entry name" value="obscurin-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000569">
        <property type="entry name" value="obscurin-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052385">
        <property type="entry name" value="Obscurin/Obscurin-like_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971:SF4">
        <property type="entry name" value="OBSCURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971">
        <property type="entry name" value="SI:DKEY-31G6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="10"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="110"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="285"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="377"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="561"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="746"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="856"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="949" mass="104789" checksum="115167F3A7B403CB" modified="2014-04-16" version="1">MDQDLFGGAPRFLTRPKAFAVCVGRDATLSCTIVGNPVPAVNWEKGKQNITSGGRFKTVEDGDVYRLTIYELTLDDSGQYICRAKNTVGEAYAAVTLQVGFPSDLVERPPTFVEKPVSTRVSLGDDVKFYCRVLAHPEPTFTWEKDGRYVGESSRFKINSDSESTSLNIQCARSIDSGTFTCRAQNSVGRAQAAAALVVEHQDSHNLASADKGSSLLSHLQKRREEMRKSDISLYRSSDLTVDNYQSSTSSSTTSILKSTEGLSSLGLSLSQDYVRAAGLASRLPKGVFTRTCTVTEGKHAKLSCFVTGHPKPQILWMKDGIVINEGRRLLIYEDEAENFILKILYCKQDDNGLYTCTASNLAGQTYSAVLLTVREPMIPFKTKLRDVEVQEKETATLQCEVPLPATETSWYMEETPLSQSAKYHMEEDGTLRRLTICNVTTNDDAVYICEMKEGSRTVAELTVRGNITKKLPRKTILPVSDTVIFCVELEKPFENCFWTRNGERLYEDSRVSLVSSDHQYTLTIRDCRAEDSGEIAFVAGDCKTSTSFSVTAPRKHPPDPPVDPVVQDKTDTSITLCWSPPVTDRPVPISGYVVERRKVGSQTWMRCHGSDNIPCPEFTVSGLTEEGSYQFRITAVNDFGQSAFLEVPGTFYLEPKASVKTPLLDVVSTAGEEATFTVELSEVCSGAWFLDGKVLRSDELYLISRTKTTHTLVIRQVRKDLNGALVRFVGGGSESTCRLKVKEEPAKFVKKPQSPAEVTVCASEDLVLSCEVAAVGTSVVWRKGHSEVKQDQRTTLISQGTQRKLVIKNARQSDQGQYTCETAEDKVTYHVKMKETTSKFVKKSTTKTEFSSCLGETVVLSCEVSQAAAQGKWMRGGQEVKLGKDVVIETEGTTRKLKIKSTKVSDSGMYTYKLPEDEITFQVNVKGKFLKKGLMSLSCGALSVPVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSZ0</accession>
    <name>W5MSZ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger, AN1-type domain 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSZ0"/>
    <dbReference type="FunCoup" id="W5MSZ0">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011499"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011516.1">
        <property type="protein sequence ID" value="ENSLOCP00000011499.1"/>
        <property type="gene ID" value="ENSLOCG00000009414.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155716"/>
    <dbReference type="HOGENOM" id="CLU_022981_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSZ0"/>
    <dbReference type="OMA" id="ECRCGHN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009414">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01802">
        <property type="entry name" value="Ubl_ZFAND4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053061">
        <property type="entry name" value="AN1-type_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46728">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46728:SF1">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="646"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="712" mass="78248" checksum="4DD50F6C25D47CFD" modified="2014-04-16" version="1">MAHKKEPPFFNEDNVGPFHYKLPFYETMELFIETLTGTCFELRVSPFETVISVKAKIQRLEGIPVAQQHLIWNNVELEDEYCLNDYSITEGCTLKLVLAMRGGPINTRRVPMDDPVKEFAEYMDSNRDDVLEKTSPNKQVTFLVYQEGDQLNFFRVVDRGDGTLTPVSESISGGSVYNLYTEEEEESEPSPSGQQIMENSITMNKMKLLKTKMENMNLSKKPKKTAKVKPRPPICARPCTDSLNTARHQRLFRVLPQIGHSLNPSTCLPPVRDQHSSATPPVPAPPSALSSVTPASQAVLSSTCYSVKEDESWECPVMTASLSKIRSPPKVSRVKVDNSHLPKVAVLPPLSVLPNRESRDGSMEQVKSNIEGTVRREDAVLVGTHPDAFLVLDEEQANASVNAEPAQGKDSSSEMIEEATERSVAALLSKAVSSESMDTWALSTERLSQRANKALSPLQFPAQLTSNPLVIGKPQRLPKSFGYGSLRPTASRALLKSEEYSGAAEASPARTPKFRGVKVDSPGKHPEIISRTEARDITEMVNKASKEPLGSVSNLGILASLARSTSKESSLPGSCGLGRPHPAALPLPTNIHLFQDDLLRKVSPPEGAGASCMVDHFGESLSANGKHRGTPTYHLPPVKAPVAPKKKSSKHCFLCGKKTGLATSYECRCRNNFCATHRYAETHDCNYDYKTAGRRYLQESNPIISAPKLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSZ1</accession>
    <name>W5MSZ1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C1q and TNF related 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011500"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011517.1">
        <property type="protein sequence ID" value="ENSLOCP00000011500.1"/>
        <property type="gene ID" value="ENSLOCG00000009423.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT1U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159591"/>
    <dbReference type="HOGENOM" id="CLU_001074_0_3_1"/>
    <dbReference type="InParanoid" id="W5MSZ1"/>
    <dbReference type="OMA" id="AMIPWVL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009423">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF28">
        <property type="entry name" value="COMPLEMENT C1Q TUMOR NECROSIS FACTOR-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866509" evidence="6">
        <location>
            <begin position="24"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="192"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="283" mass="30315" checksum="1100560D0AF4E44F" modified="2014-04-16" version="1">SPAAAPTMLCPVVLACLLPLAAHQIVSTASRKSKVLTIDSSQLACSLPGPPGPPGVPGAPGVTGTIGRMGFPGKDGKDGKDGDKGEKGERGEEGGIVGLPGRNLPPPPHPWAVSFILHLPKSWKPMVHVFGVADYGIWGGGCTLSQTLGAVPGSDTRLVSRERIPINPNQITAPVELGSLSSEVPAVVFRXYYFTYDITLANKHLAIGLVHNGQYRIRTFDANTGNHDVASGSTVLRLQRGDEVWLQIFYSEQNGLFFDPYWTDSLFTGFLIYPDQDFLNEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU27</accession>
    <name>W5MU27_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="8">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF-3-theta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF3A</name>
        <name evidence="8" type="synonym">EIF3S10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU27"/>
    <dbReference type="FunCoup" id="W5MU27">
        <property type="interactions" value="1902"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011886"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011907.1">
        <property type="protein sequence ID" value="ENSLOCP00000011886.1"/>
        <property type="gene ID" value="ENSLOCG00000009729.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111063"/>
    <dbReference type="InParanoid" id="W5MU27"/>
    <dbReference type="OMA" id="EHITNKR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009729">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03000">
        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="315"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="790"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="558"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="671"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="790"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1093"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1117"/>
            <end position="1253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1264" mass="151374" checksum="819CCC1335473F3F" modified="2014-04-16" version="1">MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVIKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTETAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSKVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLGQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFALSKKPPKPQLMANYYNKVSTVFWKSGNALFHACTLHRLYHLSREMRKNLTQEEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVDKHRRLATLLGLQAPPTRQGLINDMVRFNLLQYVVPDVKELYNWLEVDFHPLKLCGRVTKLLNWVRDQAEKEPDLQHYVPHLQNNTILRLLQQVAQIYQSIEFSRLASLVPFVDAFQLERSIVDAARHCDLQVRIDHTSRTLSFGSDLNYSTKEDSPVGPFLQNMPSEQIRNQLTAMSSALAKAIDIVKPPSILQEREEQNQQAIAAYLKNARKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERIMQEHQEIKIKTLRERLEQIKKTELGAKAFKDIDIEDLEELDTDFIMAKQVEQLEKEKKELHERLKNQEKKIDYFERAKRLEEIPLIKKAYEEQRIKDMELWELQEEERINNMKVEREKALEHKQRMSRMMEDQEYFVSKITAARRSVYEEKLKQFQERLVEERKKRLEERKKQRKEDRRNTYYREKEEEAQRIHEEQLKKEREEQERIEREQREEEEREYQERLRKLEEQERKQRARQQEIEERERRKEEERRMPEEKGMKRWADKDEGGWRKRIEGGESDWRRPVPDRSDDDKPAFRREEEREPPFRRGGDDRGPRRGISDDRVPRRGFDDDWAPKRGVDEDRALKRGVDDDWGPKRGVDDDWGPKRGVDEDRVPKRGVDEDRGPKRGVDEDRGPRRGMDDDRGPRRGFDEDRGPRRGLDEDRGPRRGADEDWAPRRGGDDERGPRRGMDDDPKPWKPLGRPGMAGGWREREKAREESWGPPRDTGCDDGDERDRDDRDDADSERFRDRRPPREESGWRRGGGEEQSWRDSTRRDDWDRGERDRRDDRDRRDLRERRDDRDRRGPPRGEREEGGSWRRAGEEKRDERRDERDVPRRAPPRERERDRDGDRGTWRSEKDKEVPRRTKNEIDDDGWTTVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV07</accession>
    <name>W5MV07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ILRUN</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV07"/>
    <dbReference type="FunCoup" id="W5MV07">
        <property type="interactions" value="1525"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012216"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012237.1">
        <property type="protein sequence ID" value="ENSLOCP00000012216.1"/>
        <property type="gene ID" value="ENSLOCG00000009988.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043415"/>
    <dbReference type="HOGENOM" id="CLU_076188_1_0_1"/>
    <dbReference type="InParanoid" id="W5MV07"/>
    <dbReference type="OMA" id="LESCNWN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009988">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14349">
        <property type="entry name" value="UBA_CF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000015">
        <property type="entry name" value="Chromosome 6 C6orf106 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000289">
        <property type="entry name" value="Chromosome 6 open reading frame 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039517">
        <property type="entry name" value="C6orf106_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="9">
        <location>
            <begin position="84"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="287" mass="31571" checksum="6478600713B4AA0D" modified="2014-04-16" version="1">PAMEGMDMDLDPELMQKFSCMGTTDKDVLISEFQRLLGFQLNPAGCAFFLDMTNWNLQAAIGAYYDFESPNINAPSMSFVEDVTIGEGESVPPDTQFTKTWRIQNTGTESWPPGVSLKYVGGDQFGHVNMVMVRSLDPQEVTDVSVQMRSPAAPGMYQGQWRMCTATGLFYGDVIWVILSVEVGGLLGVTQQLSSFETEFNTQPHRSVEGNFNPFASPQKNKHPSSDEDNSLKDPGGPWEATPDRIQQDQNGLSHNSVNIAPNGLQNNLSVVTYSQGIHGPYPFGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVY5</accession>
    <name>W5MVY5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-binding LIM protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Actin-binding LIM protein family member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with F-actin and ABRA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVY5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012544"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012567.1">
        <property type="protein sequence ID" value="ENSLOCP00000012544.1"/>
        <property type="gene ID" value="ENSLOCG00000010240.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="InParanoid" id="W5MVY5"/>
    <dbReference type="OMA" id="NTNCAYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
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        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
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    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LIM2_abLIM"/>
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        <property type="entry name" value="LIM3_abLIM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LIM4_abLIM"/>
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        <property type="entry name" value="Actin-binding LIM protein family, member 2"/>
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        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AbLIM_anchor"/>
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        <property type="entry name" value="Actin-binding_LIM"/>
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        <property type="entry name" value="Villin_headpiece"/>
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        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="LIM"/>
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        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="84"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="152"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="15">
        <location>
            <begin position="636"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="362"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="416"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
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        <source>
            <dbReference type="PROSITE" id="PS51089"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="704" mass="79152" checksum="FE507E109682E8C7" modified="2014-04-16" version="1">MPEEKVLQQQAVHSPLEKQQGTSILCHNCGKACKGEVLRVQNKHFHLKCFVCKVCGCDLAQGGFFVRQGEYICTLDYQRLYGTRCFSCDEFIEGEVVSALGKTYHPKCFVCAVCRQPFPPGDRVTFNGKECICQKCSQPLPANSPAPVQVVRNCCGCGKDFKNEQSLVALDKHWHLGCFKCKICNKVLNAEYISKDGVPYCEVDYHAMYGIQCEQCKKYITGKVLEAGDKHYHPSCARCVRCNQMFGEGEEMYLQGSSIWHPPCRQAARVEEKHKKQVRILLSDGSSQQVTRTSSESITSVPASSTSGSPSRVIYAKLGDELLDYKDLAALPKVKAIYNIDRPDMLTYSPYISYISEDRHYGESPQLLSPTPTEGDHNDKSPGQRRASSPGSHSSLGYGRYTPSRSPQHYSRPGNSQLCTHGSLQLPPVMVETLYCMCRESQLPSSRPLYCSSGPTNTNQMGLGGQFIPTAVSTTQHLCTELFVCPLGSKRKKSKIPVFKGSESGRSTPSLSAYSDSKSPSYVQAPRHFHIPDTRTKDNIYRKPPIYKQHVLRTSWQDGDDAMKTSWLILKSEMDARSGSDDIDPRTSTCSLPIDSSQPSFPYSKSASLPGYGRNGIHKASDLTSYEAEQYSSWGMREYKVYPYEVLAVTNRARVKLPRDVDRTRLERHLSPEEFHNVFEMTIEEFDRLALWKRNDLKKKARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWA8</accession>
    <name>W5MWA8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">E3 SUMO-protein ligase RanBP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Ran-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the RanBP2 E3 ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012667"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012691.1">
        <property type="protein sequence ID" value="ENSLOCP00000012667.1"/>
        <property type="gene ID" value="ENSLOCG00000010333.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154389"/>
    <dbReference type="InParanoid" id="W5MWA8"/>
    <dbReference type="OMA" id="RCIGNHG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010333">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051168">
        <property type="term" value="P:nuclear export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14684">
        <property type="entry name" value="RanBD1_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13177">
        <property type="entry name" value="RanBD2_RanBP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000018">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000020">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000114">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022011">
        <property type="entry name" value="IR1-M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12185">
        <property type="entry name" value="IR1-M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="28" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="19" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="27" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 26" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="TPR" evidence="27">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="1132"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1304"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1412"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1481"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1532"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1587"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="1813"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="2109"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="2696"/>
            <end position="2831"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="30">
        <location>
            <begin position="2852"/>
            <end position="3008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="737"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1036"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1104"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1667"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1994"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2075"/>
            <end position="2107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2276"/>
            <end position="2312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2344"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2391"/>
            <end position="2417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="2558"/>
            <end position="2620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="28">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="1110"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1678"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1708"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="1733"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="1999"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="2075"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="2093"/>
            <end position="2105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="2355"/>
            <end position="2364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="2399"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="2586"/>
            <end position="2596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00061164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00070141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3009" mass="332511" checksum="D04160375C66CD28" modified="2014-04-16" version="1">MRRSKADVDRYVSSVQSSSPSLKEKPVKGFLFAKLYFDVKEYELAKRHVSAYLTVQERDPKAHKFLGQLYEEEGDIEKAVGCYKRSVDLNPAQKDLVLKVAQLLCSKDEPDGRAEYWVEKAAKFFPGSPAVYNLKEKLLTAKGQRGWNELLDLLQSELQAQPGDAHVNVRLVQLYRLDGRLEEAVRHCLGAERKGVLRHSLPWYTTVVQTLQDYLSLPAMSPNGKMWRRVQCELLLAHCNVLRLALSEQSVQDSVEALKSFDQAMQSVKKMATCTTDDLSEIFAEMRGHIYMHVGTLLLKMAQDRKQNWRAVTDLATLCYLVAYQVPFQFFLSFKIYTMCYEITYYLLNLCSFIALRIRGCRQMSKPGDVCPTCNVNKQLKQTRFLPSKTSLSPGSVILHGGNLQHLAWLGLQWAVMDHRPALRDWLKQLFPRLTLETSKLDTNTPESICLLDLEVFLCGVVFTSHIQLQETSNIAPSSQLQEPMCLPLQTIKLLSTDRQRSWWEAIYSLIHKKALPGTSAKLRMIVQHGLTTLRAMEKHGLQPSLAIYWAQHLAETGRNMNSYYDQKEYIGRCVHYWKVVLPLLEKIKKRRNIPEPLDPLFVHFHSKDIQVTEVKECEEQAQIAFATLLDIEGKTEDAIAALEAINSVTSNWQLAQIFQRLAEEAGNGVEENKERCTNYLQKYRQHLSEIFKANVAEVDKLPIAMEDMFDLLKDVNRQLGEAGDAIHLSEPVGASSRIKFSTPSPTKTVPSPSKRHVFSPKTPPHWAEDQKSLLQILCQQVEALKNEVHDLRHNSSGTAASPHHRLYSEAYTGDAIPEAFPAAQSFHGAPLTVSTTGPSVYYNQSPAYNSQYLLRPAANVTPTKGPVYGLNRLTPQQHMYAYQQPTHTPPLQTTSACMYSQDVYGPPLRFESPATNLLSPYSEDYYNHTVPQPSTNPPLPEPGYFTKPSAGVQPPKAAEGKTVEFGKITFGQQIPLEGPKGTGFAAGVAAQSTPAATFKFNSNFKSNDGDFTFSSAQVKSNSESLLGLLTSDMPAKAEGNLGQKGPPQEPTPSQGGIFSFSSKNTSGFSFAEAAAQSQNRPGLFGKTDQPFSFKDVSKSVFSMASAEQQEGRGGESDNESMHVEEDEDGPHFEPIVPLPEKVDVKTGEEDEEEMFCNRSKLFRFDTETKEWKERGIGSIKILRHRTSGKVRILMRREQVLKICANHYIMPDMVLKPNAGSDKSWVWHAIDYADEMPKPEQLAIRFKTAEEASLFKAKFEEAQRVVTTKGASPLKQDHQMRKEASPSAQVATRDSGFGAQFAKKEGEWDCSVCYVRNTASAVLCVACQTSNPNAKAQPGTKSPMEAAKEFTMSAPGSFKFGFGKDASKVTSSAGFGAQFGSLQMPTTFNFGSCTETSKAPIPVGFGAQFDKKPGQWDCDVCAVRNEACVSQCIACNSRNPANQATVPAPAAATPSQFNFGVNSNKTTSVITAGFGDKFSKKDGEWDCDMCLVRNTSSAVKCVSCQTPNPNSKADSTKSSAFGQSGFEAPFVKEGQWDCESCLVRNEASNTKCLSCQTARKGSAFTFGISSDKSKSLQNDLAAQFAMKEGQWDCNTCLVRNEGSSAACVSCQTPNPNAKSSTSATPSSSSFTFSLSEAATKQPPPTGFKANFDTSSTLKFSQVADKSSSGSFKFEPLPSQASNKTTSSGGFSFSMPVPPGGFKFGTSEVGKQTSTAENPTSNFAEQHREKEKASSTGTTTTLTSGKPNTFTFADLAKSSQEGDFQFGQNDPNFKGFSGAGAQLFSSSQAEPKADGPVDQDEEDMYKTEENDDIQFEPVVQMPAKVDLITGEEDEEVLYSQRVKLFRFDAESSQWKERGVGNIKLLKNQENGRLRVLMRREQVLKVCANHWITTTMNLKPLSGSDRAWMWLANDFSDGDAKLEQLAAKFKTPELAEEFKQKFEECQRLLLDIPLQTPHKLVDTGRTAHLIQKAEEMKSGLKDLKSFLTDDKAKIKEDDTQAGCSTPSSTSGLIIKPHAESTGPTLEWDNYDLQGDALDDSAETSVYASPLASSPVRKNLFRFGESTAGFSFSFQPVLSPSKSPSKLNQSRVSVGTDEDSETTQEEERDGQYFEPVVPLPDLVEVSTGEENEQVVFSHRAKLYRYDKDLSQWKERGIGDLKILQNYDTKRVRVVMRRDQVLKLCANHWITKDMKLEPMKGAERAWIWSAFDFTEGEGRVEQLAVRFKLQEVANSFKELFDEARKAQENDALVAPKTPEEKNLHKSPCGKAAVAVLEETTRDRTDLPPQGDPAPGDTVSDAAPHSPDKPSKAMISPPKFVFGSESVKNIFTKSVSFGSTAPSKELFGFSFQAPPKEGTGPSSASTQAAAESVHAFETGNAKENPALAVHPAASLGGKETVPGQAQPESSAAAAESAPEEGNLKATGSAEVLIVFEQQPTREQEEMARSLMLPCTFFCYKNKPGYMSDDQDDDEDYDTAVKKLNGKLYPDQPERQRKSEETAQAVEADPDCVIVWEKKPTPEEEKKAKDLLLPPTFFCGVSSDSEGEKPDDFEVEVRRAAQALERQEGAGSTSTEAAVSTGGDVKAAGDQAASTGARSSFPSEIAPSAQDSRPIDLSTKKTCEPGSTGSEVPIVFGFGASLGFSFADLAQNSDEFAFGKKDDNFTWSNAGAAVFGCSAVTQSEKDDEGSDEEDTANNVDIHFEPIVSLPEVDVKSGEEDEEILFKERTKLYRWDRDLNQWKERGVGDMKILYHPQKRCCRVLMRREQVLKVCANHVITQAMELKPMSTSTNALVWTATDYADGDGKVEQLAAKFKTAELAESFKRKFEECQRRMSQMDSVQLSQALELSREDNPKVFFDITIDSQPTGRVTMELFAHIVPKTTENFRALCTGEKGFGYRNSKFHRVIPDFVCQAGDITNQDGTGGKSIYGERFEDENFDVRHTGPGLLSMANNGRDTNTSQFFITLKKAEHLDFKHVAFGFVKDGMEIIKQIGELGSVTGQPRKKIVIANCGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXJ6</accession>
    <name>W5MXJ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Voltage-dependent T-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This channel gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.</text>
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    </evidence>
    <sequence length="2303" mass="257968" checksum="797DCFC5DC931ADC" modified="2014-04-16" version="1">MLVILLNCVTLGMFQPCEDSDCVSERCTILQDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFTVPRSLELNSYYHTENDDENPFICSQPRENGMRYCRHIPTLRENGLECHQDYTAYNSTDNTTCVNWNQYYVNCSAGDVNPFKGAINFDNIGFAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFMSNASTLASFSEPGSCYDELLKYLVHVVRKASRQLGHVYRTVAAKTGLRLCARTGEEQPAHKRRRRKHRRASVHHLVHHHHHHHHHYHVGNGSLRTSRPSHELGDGEMGSFHNGTGRFMLPPPSTANLHLPSTQPPTSGNTESVHSVYHADCHFEPIHCTPSPSLQCIKRNSVPSASMPKNYPTVHPSTPQELQKEKTLGDSSSSGCSGGMLTNLNIPPRPFGTMNRLLETQSAGPCKGSCKLNSNCTNLDNLTFDPESCPYCIKSINNESEGTDNETLDSDSEGVYEFTQDAQYRDHREPKRRGSKVGAQAGHVLQFWRLVCETFRKIVDSKYFGRGIMFAILINTLSMGIEFHEQPDELTNALEISNIVFTSLFALEMLLKVLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSEKDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPWAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKKEDMHGQLSCIQLPVDLGGDASKSDSETDFYSHSMEDVRKSKKEVSTSAVVPINGHVEMKNSLTPPLITHTAATPMPVPKSSGFGDPALGHESRRGSSSSMDPNCYDIKSPSSARSSPYAPWSTASSWNSRRSSWNSLGRAPSLKKQKRQSGERRSLLSGDGQSSSEEDEGSECDGGSASSSLQRHRMESLETKSSFELPPDTLQVPHYLCRSASLHSTRPPLPERQDCNGKTSPTATVPQFSLDDAQSEDDNGEDEVNMGRLARIKRWCEKHQPECCRHRNSWSLYLFPPQSKFRMTCHKIITHKMFDHIVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTVIFLAEMTVKVVALGWCFGEKTYLKSSWNILDGLLVLISVIDILVSMISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCLQANFKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEAEEAKRREEKRMKRMEKKRRNVMLTDVNWESSGCAVTEAQCKPYYSDYSPTRLLIHKMCTSHYLDLFITIVIGLNVITMAMEHYQQSKVLDEALKICNYIFTIIFVLESVFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVIQALPQVGNLGLLFMLLFFIFAALGVELFGDLECNEDHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCSQDSGTCYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAEMEAELELEMQNIGGPALLGEGLPWVAGEIRDRPDSPGGGACDPGQVKVDSPLSLEPPMGSLEGELSLMDNLSGSIYHHYALPPLPSKCTGEKQIPLAEMEALSLTSEKSWSLALTDDSMPDDINTLLLTSRDGNTQLLETQREPSVQLAENLLCVRKPAVGRTHSLPNDSYMFLPQHSGDSSSPQKVSPQKGQSGSITSVQSQPVESSACHLAVPTDLFRPISPHTHSITKSIPRIPPPHRSQTASRTLRRQVAVTNDSLDALCTEVEEDGATGQSQQNELSVLGFPSQPAVLLVPATPGASPEAVRSSVRTQHAPHDQCNVSKCPPPAPAPPAPARRNPFGGQEDPVDQEVSNIIMAGLRVERGPCLRELKKYHSVDTQGRRLLLARPASWLDDPRRHSIEICSSVESSPQRSSGSSGFVSRAESFQQPSPRHKKKMSPPCISVEPPDCQELSMNFHAGVPPPRPGRENCLRRRAPSCDSKDSLDLGGEGLPPDSSPNPKLLTLPSFSFEQTDAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYD8</accession>
    <name>W5MYD8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 236</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYD8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013397"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013426.1">
        <property type="protein sequence ID" value="ENSLOCP00000013397.1"/>
        <property type="gene ID" value="ENSLOCG00000010926.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVFM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015525"/>
    <dbReference type="HOGENOM" id="CLU_072609_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYD8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010926">
        <property type="expression patterns" value="Expressed in intestine and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020394">
        <property type="entry name" value="Uncharacterised_FAM23-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="154" mass="17285" checksum="CDBA17A5DF62C065" modified="2014-04-16" version="1">MGSGKKIKFAFCEIVQFAALCIPVFVVMQRFASIVKQVKEAGMQDGDASTAYWLIVASSVAYVTSVALVIWVPMKYMIAKKRKFFTARKGWRPVTLVYVILSTLPCFAFLIASSEVQINAAERFDMLSELPVSLVLLSFICVDIIDRLRKCRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYI3</accession>
    <name>W5MYI3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Hyperpolarization activated cyclic nucleotide-gated potassium channel 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYI3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013442"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013468.1">
        <property type="protein sequence ID" value="ENSLOCP00000013442.1"/>
        <property type="gene ID" value="ENSLOCG00000010952.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156523"/>
    <dbReference type="InParanoid" id="W5MYI3"/>
    <dbReference type="OMA" id="WESFNVF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010952">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF8">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="493"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="637" mass="72045" checksum="FECF7F086974708F" modified="2014-04-16" version="1">PPTPTPGSPPLKTNPTPAMEKKGGQPPGDRSGRDSRGPSKKSAGRAAEADAKLGPLPPSPSPSPSPQHHQQDVAVSVAAPGSQAEAEEEQQQQAPAPAPAPAGCSCPGLKALFLPKLNKHSLYVYGSEGALERECARQDQLREYLFHTFIFFHPRNYYIMVMVVMTFLNLIAIPIGIAFLDGSPSSRGFDAFNLASDTIFILDIGVNFRMGVITDDSEVVILNPKEIRNHYLKTWFALDLISAFPIDYLALIIEKIEHQSDTTSYSASKLVRIIMFARIFSLVRLLRVSRLMRFFNEWERVSNANMEAVRLFIRIICLFSMILLLCHWNGCIQFFIPVLQNFPKDCWVVKENLTNASWVEQYSFGVFRALSHMIGISYGSVDPPTGEGELWVVMTSMVSGALMYTMMVANVAAMMTNVDAPSKAYRNRFNYLEDYMTYRKLPRDLRTRISNYYQARHQGKWFDESEILNLVSKSLREEILNILCADLLNSVPIFKNRDINFINAVLLRLKYEVFQEGDIIIRQNAPGDRMFFIGHGKVLVETPTFKKELIDGDYFGEICLLTKCKRTATVRALSLCKVFSLSSECFWRILQGFPEIKEELEKTARERLLYLRESQIKHEQQVLLKCVTKDTAGETSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYK9</accession>
    <name>W5MYK9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYK9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013468"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013497.1">
        <property type="protein sequence ID" value="ENSLOCP00000013468.1"/>
        <property type="gene ID" value="ENSLOCG00000010980.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2J8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154967"/>
    <dbReference type="HOGENOM" id="CLU_020107_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYK9"/>
    <dbReference type="OMA" id="EPPKCKV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010980">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF15">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867005" description="Sushi domain-containing protein" evidence="4">
        <location>
            <begin position="31"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="93"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="206"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="319" mass="34734" checksum="09DB1A1AB36D6AB9" modified="2014-04-16" version="1">MSGWGRFCEQAVLQNGLVLILTVLVVNVAGQCSNPPSVQNAVLNDQSQLLNDFPEGTAVTYECNPGYRRASGSPSISCSNGQWSQPTLTCEKKSCGSPGEILNGRYDLSEGILFGAKARVICDEGYQLVGRERLCFADGWSSDVPLCEVVSCPDVSITNGKKLPPYGYKSFITFECDSGHKVNGTLTITCGQRLRCKDGNYHPSPPQCKVVSYPDVIITNGRKVSERLPPSGYKSFMTFECNPGYTLIGTRTITCGVNGWSTNPPACEKVLVHQLAGPPAEELTSWRIISEVMVKRGMTCLVIIIPFLYSTATQESYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYQ3</accession>
    <name>W5MYQ3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C-Jun-amino-terminal kinase-interacting protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">JNK-associated leucine-zipper protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Mitogen-activated protein kinase 8-interacting protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Sperm-associated antigen 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex. Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013512"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013541.1">
        <property type="protein sequence ID" value="ENSLOCP00000013512.1"/>
        <property type="gene ID" value="ENSLOCG00000010987.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2077">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153496"/>
    <dbReference type="InParanoid" id="W5MYQ3"/>
    <dbReference type="OMA" id="DXASREN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010987">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1770:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000700">
        <property type="entry name" value="Sperm-associated antigen 9a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF2">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RH1" evidence="17">
        <location>
            <begin position="6"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="18">
        <location>
            <begin position="506"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="864"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1256"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="60"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="437"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="735"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="864"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1335" mass="147731" checksum="286F095DE95C7719" modified="2014-04-16" version="1">MELEDGVVYQDDPGTAAMMSERVSGLASSIYREFERLIGKYDEDVVKELMPLVVAVLENLDSVFAENQEHEVELELLKEDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQGRVETLESQSRQLELKAKNYADQIGRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKFQQLTGGDPSETGTLGRVRKERPVSLGIFPLSGADPLLTADTRQGGAETPGTENWRFNDLSHPRSNTSLKVYQGFNTWVLKYSAVMDYKEEIESADQAPLTTATVVSSRTKSIFFSTATPFISSLPKQLEAEGVLKERDGKRSQEAWGNSLAPERKRSVSIGLADNEEKSEVQAIIESTPELDMDLSGYKGASTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNVVKNDLIAKVDELSCEKEVLQGELEAVKQAKTKLEEKNKELEEELKKVRAEVEEAKQKSKEEDDSDVPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRSEKYPSLSRKREGLLFSPDNFSRLFNPSSNTTKKPEPPVNVKYNAPTSHITPTVKKRSSTLSQLPSDKSKAFDFLNEEIDSGNLASRREQKRAQYRQVKDHVQKDDGRIQACGWSLPQKYKVANGGQAESRMKNLPVPVFLRPLDEKDASMKLWCAAGVNLSGGKTRDGGSIVGASVFYNDVASSEAERQRQRKGSQSSLDRLEQELKEQEKELRQHEELSSLVWICTSTHSTTKVMVIDANQPGNILENFFVCNSHVLCIASVPGARETDYPAGEEVPQEAEGGAADNASLTASVASSGSAGSDSMLGGITVVGCTTEGTATIPQTESTPVSASPIPDVQTGETGAENSAVEGGVPTAEEATEATEASAGSGDDGADISQPGIYTEHVFTDPLGVQNSAESTPVYPQRCRKLKLSQDGVTSLPEEQDLMREEAQKMSSVLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGRVLVALADGTLAIFHRGVDGQWDLTNYHLLDLGRPHHSIRCMSVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLIETNKVATGTAKGPGSVIRVYGDENSDKVTPGTYIPYCSMAHAQLCFHGHRDAVKFFAAVPGQVICSSASSGSDTLTDKPGSSASEPSSQDKLVKSVLVLSGGEGYIDFRMGDEGSAAEESDTTLQLQPFLAKAERSHLIVWQVMLSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZM7</accession>
    <name>W5MZM7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyltransferase 23, arginine</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily. METTL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211453.1">
        <property type="nucleotide sequence ID" value="XM_015355967.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZM7"/>
    <dbReference type="FunCoup" id="W5MZM7">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013836"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013865.1">
        <property type="protein sequence ID" value="ENSLOCP00000013836.1"/>
        <property type="gene ID" value="ENSLOCG00000011260.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689537"/>
    <dbReference type="CTD" id="124512"/>
    <dbReference type="eggNOG" id="KOG2793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048008"/>
    <dbReference type="HOGENOM" id="CLU_082022_1_1_1"/>
    <dbReference type="InParanoid" id="W5MZM7"/>
    <dbReference type="OMA" id="RADWSIE"/>
    <dbReference type="OrthoDB" id="407325at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011260">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035642">
        <property type="term" value="F:histone H3R17 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF164">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE METTL23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="200" mass="22349" checksum="E7335E82812A231C" modified="2014-04-16" version="1">MSEAEELRTGLKQFKFEDEGQTESQSLIVSIPEVVDPQYGMYVWPCAVVLAQYVWGHKEELGGKDVLEIGSGVSLPGVVAAKCGAHVILSDDAERPQCLENCRRSCEANGLSDMRVMGLTWGRISPDMLLLPPLDVILGSDVFYEPEDFEDVLVTISFLLRRSNRAQFWMTYQERRLVHRSSPPQVEPAVCSHSSRSISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N061</accession>
    <name>W5N061_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c oxidase assembly factor COX18</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N061"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014020"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014049.1">
        <property type="protein sequence ID" value="ENSLOCP00000014020.1"/>
        <property type="gene ID" value="ENSLOCG00000011403.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063506"/>
    <dbReference type="HOGENOM" id="CLU_029282_2_2_1"/>
    <dbReference type="InParanoid" id="W5N061"/>
    <dbReference type="OMA" id="WQRKRIV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011403">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="7">
        <location>
            <begin position="75"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="323" mass="35824" checksum="286F659C06D32713" modified="2014-04-16" version="1">MACTASCHDQRLPSREGVRLSRSPGSAVLQSRGLVSAASAASAGPSWYESLADSAPVHLTEGLLAVTQQATGLPWWASIACTTVALRTAVTLPLGTYQMAIIAKIESLQVEIGELAKRLRYEVSVRAKQLGWSEKVARYHFRKNLRRIVSELYVRDNCHPVKASLLIWVQIPMWVFLSLALRNMSLGVSDSSRAFQSELAAGGALWFPDLTLPDPTWIMPVSLGLVNLIIVEIFSLRRTEVSRFQKYITNFIRGISLVMIPIAATVPSSMTLYWLSSSCVGLGHNLLLRSPRFRRICRIPPSRADSETPYRDLASAFVTKYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N079</accession>
    <name>W5N079_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N079"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014038"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014067.1">
        <property type="protein sequence ID" value="ENSLOCP00000014038.1"/>
        <property type="gene ID" value="ENSLOCG00000011420.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157509"/>
    <dbReference type="HOGENOM" id="CLU_042804_0_0_1"/>
    <dbReference type="InParanoid" id="W5N079"/>
    <dbReference type="OMA" id="KWILYHV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011420">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="394" mass="44227" checksum="F2EC0B068079868E" modified="2014-04-16" version="1">MSRSQIAEAVAGERSGHTAVADAGYVYVWGGYVSIAEHGVFYPSDEIWLYDVDSGIWQAHPMEGDAPPPMSGTCGCSLNGEMYIFGGYNDNGYTNQLYCVNLRSEEYTWKKVSYAEGCQPTPRDKNSCWVYNDRIIYFGGYGCKRFSEISDSTSFTVDEVSWVGEIFWGWNNEVHVFDPQKLTWSEPSTRGVVPAPRAAHACATLHNKGYVCGGRVVNTRTSDIHCLDLDTWTWSEIIHTSAQVPAGRSWHTLTPVADSVLFLFGGLSANCEPLSDSWTFNTHSREWREMKHLQKNTPRLWHTACQGKGSEVIVFGGSQDDLLSVDTQGHCNDILVFQTQPCSLVRLCEDCIGKNSRVLNEQLSWLPLKLRQAIQKRILFFEIAEKSTRKDNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2Q2</accession>
    <name>W5N2Q2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nebulin-related anchoring protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014911"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014940.1">
        <property type="protein sequence ID" value="ENSLOCP00000014911.1"/>
        <property type="gene ID" value="ENSLOCG00000012095.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1702">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158418"/>
    <dbReference type="HOGENOM" id="CLU_003010_1_0_1"/>
    <dbReference type="InParanoid" id="W5N2Q2"/>
    <dbReference type="OMA" id="NERPYWT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012095">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071691">
        <property type="term" value="P:cardiac muscle thin filament assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060297">
        <property type="term" value="P:regulation of sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09446">
        <property type="entry name" value="LIM_N_RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000110">
        <property type="entry name" value="Nebulin related anchoring protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055297">
        <property type="entry name" value="NEBU/NEBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013998">
        <property type="entry name" value="Nebulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11039">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11039:SF39">
        <property type="entry name" value="NEBULIN-RELATED-ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00510">
        <property type="entry name" value="NEBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="45"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="35"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1764"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1764"/>
            <end position="1773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1788" mass="206120" checksum="5BB04467241C5EAD" modified="2014-04-16" version="1">MNVQPCARCGYIVYPAEKINCIDKTWHKACFHCEICKMILTTNNFVSHQKKPYCQVHNPKNNTFTSVYETPINLNAKKQSEAVSELKYREEGEKYKSVFHWDVKSREVEHARKISHLASQKAYQAEYEEQKSWYTGTASNPEMIRVAQAQKTISDVEYKRGHEERVSQYTSVADTNARLSHLYCSFSVTVKIKYTEEYEQLKGKGSFPAMITPAYQIAKKANTLASDVEYKRGHEERISKYTSVTDTPEVLLAKTGAQLASDYMYTEEYEQHRGKGSFPAMITPGYEVAKKATELASDIKYHQKYEREMKGKGSSAAGSAELALARDNAEKFSQHAYTEDYEEHRGKGSFPAMITPAYQIAKKAHELASDVKYKQGFSKTKGTAHFNTLTADDNLVIKNAQKINKLVSEVEYKKDLENTKGHSINYCETPQFKNVSKISKYTSDVKYKENYLNQMKGHYEGVGMDKRTLHAMKVKNLASNIAYKSEYEHEKSEHADYNYPATMTPSYETQRKLEPLKDVNYRQHVDKLKYSSVTDTPEIVQARINAQQFSDLNYRAEYEKTKTKFTLPQDLPQIKKAKANAELFSDIKYKENWEKTKSKACEMSLDDLSLQAAKASRDLASDIKYKEDFMKIKDKAVGVNMSDSRTLHSLQVAKMNNEIAYKKGSKEARSQFHLPLDMINISHAKKAQSLASDLEYRKRLHEYTVLPDDMKVKWAKKAYNLQSENQYRADLLWMKGVGWMTEGCLDVQQAKKAGELVSEKKYRQKADAVKFTQVADTPYIKHAKRSQDLQSGIVYKAGTEQMLHQYTMSKDEPLFKLAKTNAEYLSEKTYRSSWEKQKEKGFELRMDALSILTAKAKRDLASDIKYREVYEKTKGKLIGVKNVKEDSQMAHSTQVSKLQSDLEYKKEYEDSKTKHSASLDMLNMVHAKKAQCLATDTGYKTILHHYTTLPTDMKVEWAKKAYGLQSDNKYKSDLNWMRGVGWMTAGSLDVEQAKKSGELISETKYRQHPYALKFTSVKDTPEMLQAKISYNQAVDRLYREHGESVKHHYTLTKDLPEHIQAKLNAMNISETCYKQSWTKLKDAGYTFRLDAIPFQAAKASGDIFSDYKYKEEFEKLKGHMIGLKGVEDDIKIVHSVHAAQLQSDISYKKDSVRGRSQYHLPLDMMELTHAKKAQSLISDQDYKHLFHNYTSLPSDLKLQWAKKAYDLQSEKLYRSDLNFLRGVAWISTGAVQIEGSKRANELISEKKYRQHPYSFKHTAVTDSPDLVHAKFSNQITNERLYKEAGENARHNYTLTLGRPELTQAKINAANFSEIKYRESWHNLRAQGYKLTMDAIAFQTAKASGDIASDYQYRHDFVLEKGKHIGARSILDDPRLLHCMQVAKLHSEQQYKKESRSTSAQFHLPLDMVHLVHARKAQALASDQDYKTKFHSYTVLPDDMKVQWAKKAHELQSEKLYKSDLNFMRGVGWITVGTPQIETAKRAGELISEKKYRQLPDSLKFTSIADSPDIVHAKNSYLQCSERLYKSGDSELMHRYTLPPDHPDFIRARMNAQHISDKVYKTSWDQVRSTGYDLRLDAIPFQTAKASRDIASDFRYKETFVKERGHQIGLRSINDDPKMKHFLSASKLQSNNEYKKQYEETRSQYKIHTDQPSFIHAKKSQEQASNLSYRQHLHHYTCDPEQLNMKHAKQAYKLQSDVNYKSDLNWLRGIGWTPPGSHKVEMARRAAELGYAQEMNPEEAAAHYQQMMDRFITIYFPQQASQMMETQEEFQQPGVNPDASEILQVKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3R5</accession>
    <name>W5N3R5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="8">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01026321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629935.1">
        <property type="nucleotide sequence ID" value="XM_006629872.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3R5"/>
    <dbReference type="FunCoup" id="W5N3R5">
        <property type="interactions" value="549"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015274"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015303.1">
        <property type="protein sequence ID" value="ENSLOCP00000015274.1"/>
        <property type="gene ID" value="ENSLOCG00000012405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698404"/>
    <dbReference type="KEGG" id="loc:102698404"/>
    <dbReference type="CTD" id="132789"/>
    <dbReference type="eggNOG" id="KOG3148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014316"/>
    <dbReference type="HOGENOM" id="CLU_049611_0_1_1"/>
    <dbReference type="InParanoid" id="W5N3R5"/>
    <dbReference type="OMA" id="MEFSKHI"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012405">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000004">
        <property type="entry name" value="Glucosamine-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01241">
        <property type="entry name" value="GlcN6P_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF9">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="277" mass="31368" checksum="F5F0F7F3939258A4" modified="2014-04-16" version="1">MRLVILEDYALASEWAAKYIRNRIIQFRPNSDKYFTLGLPTGSTPLGCYKKLIEYHKNGDLSFKYVKTFNMDEYVGLPRDHPESYHSYMWNNFFKHIDINPANAHILDGNSHDLQAECDAFEEKISKAGGIDLFVGGIGPDGHIAFNEPGSSLVSRTRVKTLAKDTIVANARFFGNDLTKVPTMALTVGVGTVMDAREVMILITGAHKAFALYKAIEEGVNHMWTVSAFQQHPHTIFVCDEDATLELRVKTVKYFKGLMHVHNRLVDPLYSMKDYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4L4</accession>
    <name>W5N4L4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4L4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015573"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015602.1">
        <property type="protein sequence ID" value="ENSLOCP00000015573.1"/>
        <property type="gene ID" value="ENSLOCG00000012647.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688662"/>
    <dbReference type="KEGG" id="loc:102688662"/>
    <dbReference type="CTD" id="147138"/>
    <dbReference type="eggNOG" id="ENOG502QPM8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244894"/>
    <dbReference type="HOGENOM" id="CLU_013958_1_0_1"/>
    <dbReference type="InParanoid" id="W5N4L4"/>
    <dbReference type="OMA" id="KKYTLMR"/>
    <dbReference type="OrthoDB" id="1936208at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012647">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF66">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="453"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="745" mass="86460" checksum="A5EEBD615831247E" modified="2014-04-16" version="1">MEETRHRNVEFFRLESGESAATSLYDEPTSYHQTAVFTQLPSSQSLRRRRGESGHAGPAGWEEEEGDRATAGFLKPREWDPNTPLRMLPLSMEQKRNTRKQRQEKQRNIGCWESWQQSQRTSRRRLRESLANKLRDLQPWKAVLHQIEGKFGSGVMSYFTFLRCLVYLNLLNVLVITGMVIVPITVYERNSTVRQREATCKLNDSFLDLFSGMGFLGRSSLFYGFYTRRSLDSACLNIPLLFLLSVGVVLLVNLVWVVVRTVNGYKLKWMMGNRYKMTKSYKIFCGWDFCIQVPDSAALRHSNIRNDLKVDLEEERFLQRESQRTVQQWVLLYFMRAVLNLVVFVLLSGAFYLIYFATQRSQRYQQAKSGWILNLFMEYLPSITITLINFLLPFIFCKIVKFEDYSVANKVNITLVRSIFLKLASLAMFLFFMYQSVRTAQQNSKSPTCEKQCWENTIGQEMYKLLVFDFLASLGNTILVAYPRKQLVERCSWRLVQMIGKDQFLIPLNVLDIVYSQTVSWVGLFFCPLLPLLNLVKMLALFYIRKFHLFRNCAPVRRMFRASSSSVLFYFMLLLGLLMSLAALGIHFVEFKPSRACGPFQEQERVLDVATVCIQSLPNATRPVLLYLVSEAFAIPLILTEIILLTFYMSIYRANCKTIERLKDRLVMEGYDKRFLVNKLSAPLQKYERRLRTDISKGSDSEDIHSWQGVTDLQDFEPVLSRGVSVESEEDEGRSLAVMSTQNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4U6</accession>
    <name>W5N4U6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rap1 GTPase-GDP dissociation stimulator 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629968.2">
        <property type="nucleotide sequence ID" value="XM_006629905.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015201306.1">
        <property type="nucleotide sequence ID" value="XM_015345820.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4U6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015655"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015684.1">
        <property type="protein sequence ID" value="ENSLOCP00000015655.1"/>
        <property type="gene ID" value="ENSLOCG00000012715.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687826"/>
    <dbReference type="KEGG" id="loc:102687826"/>
    <dbReference type="CTD" id="5910"/>
    <dbReference type="eggNOG" id="KOG4500">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014293"/>
    <dbReference type="HOGENOM" id="CLU_021124_1_0_1"/>
    <dbReference type="InParanoid" id="W5N4U6"/>
    <dbReference type="OMA" id="NGTEHQM"/>
    <dbReference type="OrthoDB" id="26149at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012715">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000127">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000141">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000144">
        <property type="entry name" value="rap1 GTPase-GDP dissociation stimulator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="357"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="607" mass="66150" checksum="C511F674B8279183" modified="2014-04-16" version="1">MDNFCEALRKLKLTEADSTVDSLEGCLDCLLKALAHNNVEASEKIHESGTLKMFPGLLSPQSSSTPKVANVIAEVAKNEFMRSPCVEAGLIPPLVQLLHCKDQEVLLQTGRALGNICYDSQEGRSAVDEAGGAQIVAGHIRSLSHSTDPASEKLLTVFCGMLMNYSNDNDTLQAQLINMGVIPTLVKLLGIHSQNASLTEMCLIAFGNLAELESSKEQFASTNIAEELVKLFKKQAEHEKKEMIFEVLAPLAENDVIKLQLVEAGLVECLLDIVAQTVDGEKEEDIAELKTASDLMVLLLLGDESMQKLFEEGKGSVFQRVLSWVPSHNHQLQLAGALAIANFARNDGNCIHMVDTGIVQKLLDLLERHAEDGNVTVQHAALSALRNLAIPVVNKSKMLSAGVSDVVLKFLKSEMPPVQFKLLGTLRMLIDTQAEAADQLGNNVKLVERLVEWCEAKDHAGVMGESNRLLSALIRHSKSKDVVRTVIQCGGVRHLVTMATSEHMIMQNEALVALGLMAALDLGMAEKDFESADLVQVLHKLLSDEKSAPEIKYNSMILICALMGSEPLHKAVQGLTFLDVVSKLRSHENKTVAHQASLTEQRLTVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5Z2</accession>
    <name>W5N5Z2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Family with sequence similarity 181 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5Z2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016051"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016081.1">
        <property type="protein sequence ID" value="ENSLOCP00000016051.1"/>
        <property type="gene ID" value="ENSLOCG00000013032.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZWV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154730"/>
    <dbReference type="HOGENOM" id="CLU_060757_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5Z2"/>
    <dbReference type="OMA" id="SRMPRCH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013032">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029359">
        <property type="entry name" value="FAM181"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053819">
        <property type="entry name" value="TEADIR3_omega_loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33766:SF1">
        <property type="entry name" value="PROTEIN FAM181A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33766">
        <property type="entry name" value="PROTEIN FAM181B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15238">
        <property type="entry name" value="TEADIR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="256" mass="28040" checksum="AE9E01C58BBBFE0A" modified="2014-04-16" version="1">MACTDSEVKTLLNFVNLASSDIKAALDKSAPCRRYSRIPRCQSYRTSESGIGKALEDKSGTYPLETVNRNLSLAHLSEKIGSDLRQDQVPMRKRQLPASFWEEPRSSKSKHETFQAVWRKNQAVGGSGLSGSVEQIRKKCSEGPKTTSVHINRQSVAEKEPLTVEMTSLTGSASLCGCCPFQYHGQHVYQSHVVLPQSGFSDSSMWGKPSVAQTEGLDSCKDVAINGQKSHTHVVVKPIPTKPTVPSPIFSVFGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6C0</accession>
    <name>W5N6C0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tektin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6C0"/>
    <dbReference type="FunCoup" id="W5N6C0">
        <property type="interactions" value="692"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016179"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016209.1">
        <property type="protein sequence ID" value="ENSLOCP00000016179.1"/>
        <property type="gene ID" value="ENSLOCG00000013131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694954"/>
    <dbReference type="KEGG" id="loc:102694954"/>
    <dbReference type="CTD" id="64518"/>
    <dbReference type="eggNOG" id="KOG2685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182894"/>
    <dbReference type="HOGENOM" id="CLU_033588_2_1_1"/>
    <dbReference type="InParanoid" id="W5N6C0"/>
    <dbReference type="OMA" id="CMEPISG"/>
    <dbReference type="OrthoDB" id="9886517at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013131">
        <property type="expression patterns" value="Expressed in embryo and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF11">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="488" mass="56630" checksum="B87F0C5FC44908B5" modified="2014-04-16" version="1">MELLGSTQTATYARPRTSNFLPAISTMASSYKSHYPHFALTHSINQPWRTNMFYKTASAIPTLASIQRVPPDLTKAKVNFYPSNRSALVNRYTPDDWHKSNQSNYRESESSRHSAERLRRDTTRLIQDKEQLTRRNQESTNKNIGERINDIAFWRSELTHETGNMIGEINTLTDVKKRLERALAETEGPLQVAQECLYHREKRMSIDLVHDEVEQDLLKEVEMIKSCQERMRRHLDRVNAQLASNRAAQHELERDVNDKAEAHRIDDKCHQLRNTSDGISYYRGVERLDPSLSIPDSWAKFTDNNILRSQSERAASARLRDEVEILLNTTSNEMWNQFNTVNVAFTNRMAETADAKNNLQTHLAKTLQEIFQTEMLIETLKKSIRDKESPLKVAQTRLEERTRRPNVELCRDNPHHRLVSEVREIGDTIQKLKERLRDAEDTLQTLVRTKVSLEHDLSVKANSLFIDQEKCMGMRKSFPSTPRLVGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6E3</accession>
    <name>W5N6E3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01002345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6E3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016202"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016232.1">
        <property type="protein sequence ID" value="ENSLOCP00000016202.1"/>
        <property type="gene ID" value="ENSLOCG00000013151.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521246_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6E3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013151">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="177" mass="19415" checksum="5A3DE03A4A4349B1" modified="2014-04-16" version="1">MGIKSGRRRWGQTALKASDSWDDFDDSDIGVSFSKKPSIGSIKEKQNDDSFFRSTEQKPVNTYGAANRIPSNTMVNRIDTGLSNSASARQHYLRQSRYLPGVNPKNTSLIANKDPSRDLWGNASSLINKPLGPIGSGLSITRVNAEDSLSKPTDKASLKEKTLEKLEEHKGTFIGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N765</accession>
    <name>W5N765_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CD276 antigen homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212134.1">
        <property type="nucleotide sequence ID" value="XM_015356648.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212135.1">
        <property type="nucleotide sequence ID" value="XM_015356649.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N765"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016474"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016504.1">
        <property type="protein sequence ID" value="ENSLOCP00000016474.1"/>
        <property type="gene ID" value="ENSLOCG00000013368.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078539"/>
    <dbReference type="eggNOG" id="ENOG502S3W7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000073890"/>
    <dbReference type="HOGENOM" id="CLU_079088_0_0_1"/>
    <dbReference type="InParanoid" id="W5N765"/>
    <dbReference type="OMA" id="PLMGTHD"/>
    <dbReference type="OrthoDB" id="9942764at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013368">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869375" evidence="12">
        <location>
            <begin position="22"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="21"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="140"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="287" mass="31703" checksum="63102B338F84AE1B" modified="2014-04-16" version="1">MDRSAWLFAVVFIVEFTRVTPSDKLVSMHCQSEHHGKFGQDTKLSCIVNSTSSEVKIAKLLWKKESQGSVLTYDYGKKKAEDPRFELAHQSWGPNNLDVSLLVRNTKISDQGAYSCVVVTDRGFCNGVTTLQVTAAYAQPVMSSIPAENIQEDSTVEIFCNTSGGYPEGRIMWHDQYGTDWTRSAQTKAVLTEDGRYNLTSKYTVLKSSSSSLEYSCSVHNSQQVKEGTQVHHLMFGPNKMEGPSNSTAVAAVFVVVGALAAGILVLFLFRRRRNSGAGDYTAREKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N795</accession>
    <name>W5N795_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N795"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016504"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016534.1">
        <property type="protein sequence ID" value="ENSLOCP00000016504.1"/>
        <property type="gene ID" value="ENSLOCG00000013390.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3FK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164625"/>
    <dbReference type="HOGENOM" id="CLU_077975_0_0_1"/>
    <dbReference type="InParanoid" id="W5N795"/>
    <dbReference type="OMA" id="RITENQW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013390">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF28">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="120"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="287" mass="32125" checksum="5B84F284D85E577E" modified="2014-04-16" version="1">VTITQKPVITFCNPGAASVTFHCEHDQSTHYFIFWYKQSQGGELFLITYSSGTGTADTEPAFNKSKFTMARPQIKKSSLEIKNLESSDTAVYFCASRRSLHCANYEAYFGQGTKLTVLDPSVPVTSPKNLKILKPSKEEIKKKKKATLVCVATGFYPDHITVSWKVNGQDQTKGVKTDDAAIKGNDKKYSITSRLRVRETEWFNPKKKFTCITSFYNGSAYMKNEVQINGELDVNSTGCGLTADAYMGSTKNVSTYYVIFLGKSVMYGLFVTVLAWKIKRNSGKHYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7R1</accession>
    <name>W5N7R1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01030366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5N7R1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016700.1">
        <property type="protein sequence ID" value="ENSLOCP00000016670.1"/>
        <property type="gene ID" value="ENSLOCG00000013524.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="W5N7R1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013524">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR038923">
        <property type="entry name" value="Centrobin"/>
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    <dbReference type="PANTHER" id="PTHR34439">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439:SF1">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="31258" checksum="75DD712069B340D3" modified="2014-04-16" version="1">MIEQLDKTLAKVVEGWRKHEADKSDTARRLQEEKETAERAQAEQQEMLSRFEESLAQAAETLDREQRQAEELRATNQRLEQQVGELSRRLEEVARRCQALEEQRDEATRAREAAHASLDQHRASWDRRERELEDRLARQEAEFMAQLDNEKASREREAQRAQDTQRVLASVQEEVQRAERELEEAQRARDSAAMDRALAEARFEAERTKMEVELKVSVEQQVTERLKQIQEENTKATAALRETHRYCLRERHTEVLSERETHTGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8F8</accession>
    <name>W5N8F8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 22 member 5-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8F8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016917"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016947.1">
        <property type="protein sequence ID" value="ENSLOCP00000016917.1"/>
        <property type="gene ID" value="ENSLOCG00000013715.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="W5N8F8"/>
    <dbReference type="OMA" id="HYDRILP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015651">
        <property type="term" value="F:quaternary ammonium group transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015697">
        <property type="term" value="P:quaternary ammonium group transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 22 member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="21"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="546" mass="60204" checksum="D269B1FB413E0524" modified="2014-04-16" version="1">MRDYDEITSFLGEWGPFQKSVFILLSLSTVPNGYAGLAMVFISDTPPHHCTVAPSNGTPVVVDLNRSLPVESVNGELRYSRCSRYKVPGGPGAGIGNNTEGCIDGWEFSTERYTSTIVTEWNLVCEEGWKVPFSLSVFFMGVLMGSFMSGQISDRYGRKVILFITMALQTVFSLIQIASTSWEMFCILFFIVGIGQISNYVAAFVLGTELLGKSARIAFSTVGVCVFYGLGYAILPLCAYFIRSWKALLFALALPGFLYIPLWWLIPESPRWLLAQGRIKEAEAIIRSAAKKNSVTPPEVIFKAEDSIRLMRQNDTEVNQHSYTYFDLVKTTDMRNITILNILIWFVITISYFGLSLNTPNMNGDPYINCFASAAMEIVAYVAAWLLLRVAPRRTIISSTLLSGGTLLLLIQLVPSNLGILASVLAMVGKFGITAAFSVIYVSAVELFPTVVRSMGVGICSMASKIGSMISPFFAYMGAYNQVLPYILMGILTVSAGLLSLLLPETRGTDLPEDLTQVQKIVCRRGEVENRNIFNAQVPANKDPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8U5</accession>
    <name>W5N8U5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Family with sequence similarity 149 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM149 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002170">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8U5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017084.1">
        <property type="protein sequence ID" value="ENSLOCP00000017054.1"/>
        <property type="gene ID" value="ENSLOCG00000013829.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063727"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022194">
        <property type="entry name" value="DUF3719"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039630">
        <property type="entry name" value="FAM149"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31997">
        <property type="entry name" value="AGAP003710-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31997:SF2">
        <property type="entry name" value="PROTEIN FAM149A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12516">
        <property type="entry name" value="DUF3719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3719" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="51026" checksum="D75A9792A37AD700" modified="2014-04-16" version="1">MLYEGKVDARTRGLRAECGEWNQNSPHLRILGNQIAEPKDEGFRLFQRRASIPESALLHPCQDTMAENKDMSVVGRKLVPVTSPLHGTLGCRRSPSEPSPYAFLEEETYEAEGQIEEFFAYDLKEVEEDGWEFRKALKINSGVPPVSPHACIKDGVIGELFDEVWKDVVHFVMELIQKHWESDLTVIQKEEDKHCDRDFSESVFRTPVKAFSVSPSRGSETRSMHTCSTQAPRIPSALKRNLNGVMTIQAKPLQHRHPGLGEKTQCEHRDPAGTGQAAETGHAVPSPSRAPCVGSRGPAAQRRLPRPPPDSVGFRRSAVLKGTKLCALPEGLSLSPECARRNRKFPPIHSEVPEQSPSVPASRHLQRRGRDLSSRVSSAVQGDSGPRAPRGQALPLEPLSRPRTSYTFRSDASFKRLFTPVDFVCQTRIGRGPFAGNSYKPRAFSRKPATGRKKFQMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAI3</accession>
    <name>W5NAI3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2 chloride(in) + H(+)(out) = 2 chloride(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with NEDD4 and NEDD4L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632789.1">
        <property type="nucleotide sequence ID" value="XM_006632726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAI3"/>
    <dbReference type="FunCoup" id="W5NAI3">
        <property type="interactions" value="729"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017642"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017674.1">
        <property type="protein sequence ID" value="ENSLOCP00000017642.1"/>
        <property type="gene ID" value="ENSLOCG00000014329.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697299"/>
    <dbReference type="KEGG" id="loc:102697299"/>
    <dbReference type="CTD" id="557584"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153763"/>
    <dbReference type="InParanoid" id="W5NAI3"/>
    <dbReference type="OMA" id="CLDWTPW"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014329">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000001">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000002">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002247">
        <property type="entry name" value="Cl_channel-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF7">
        <property type="entry name" value="H(+)_CL(-) EXCHANGE TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01116">
        <property type="entry name" value="CLCHANNEL5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0050">Antiport</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18 23" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20 24" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 24" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="25">
        <location>
            <begin position="650"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="25">
        <location>
            <begin position="747"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00029360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="810" mass="89852" checksum="3E3ED85C68B307F0" modified="2014-04-16" version="1">MDNQGYCGESFNSLQSGTSDEDMVEIAGATLDFSVTDDVPPLDREYAGGYNTYTGEGMNGTSKLMDFLDEPVPGVGTYEDFNTIDWVREKSKDRDRHREIASKSKESTWALIQSVSDAFSGWLLMLLIGLMAGALAGGIDISAHWMTDLKDGVCLNGFWFNHEHCCWTSNETTFMERDKCPQWKSWAELIIGHSEGAFAYITNYLMYVSWALLFAFLAVSLVRAFAPYACGSGISEIKTILSGFIIRGYLGKWTLIIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHLFTKYRKNEAKRREVLSAAAAVGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSKLVLFYVEFHSPWHLLELIPFILLGIFGGLWGAFFIRTNIAWCRRRKTTKLGHYPVLEVLVVTAVTAILAFPNDYTRISTSELISELFNDCGLLDSSKLCDYINLNSSKSSNELPDRPANSNVYTAMWQLALALIFKVLITIVTFGMKVPSGLFIPSMAVGAITGRLLGVGMEQLAYYHHDWVIFKGWCTPGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADAIGREGIYEAHIRLNGYPFLEAKEEFGHKTLAMDVMRPRRSDPPLTVLTQDHMTVEDVETLISETTYSGFPVVVSHESQRLVGFVLRRDLVISIENARKRQDGIVSTSHIYFTEYMPPQPSTSPAQLKLRSIMDLSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDILKHMAQMANRDPDSILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBM6</accession>
    <name>W5NBM6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023778">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBM6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018035"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018067.1">
        <property type="protein sequence ID" value="ENSLOCP00000018035.1"/>
        <property type="gene ID" value="ENSLOCG00000014652.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166445"/>
    <dbReference type="InParanoid" id="W5NBM6"/>
    <dbReference type="OMA" id="PGEGWQD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014652">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000007">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000014">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="607"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="357"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="433"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="471"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="508"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="717" mass="76686" checksum="942D799D0EBD439C" modified="2014-04-16" version="1">PPCRQPAGGAPVQDGWSGSGADRESLTTSLSAALLPLQVLQAVGVFELQIEQLQNTNGLLLGGGCCDGGGGGGAGGRCPQADQCDTFLRACLKEYQVRVSPTGPCTFGAGSTGVLGGNSFSLRHRTPGEQVGRIGIPFKYAWPKSFSLVLEVLDHDNDTVGAGRTELLENVVAQCCLCPGLTWASGSYSDLPDMWVLGRQGCLSQYCSSNLRGPVCICTEAALKSLFSHICADCSVEKRCYTTPPTGGPEVRVPAVCRQGCHLAHGYCSTPGECKCHYGWEGPLCDRCVTFPGCVHGSCTEPWMCVCDTNWGGLLCDKECGFSRGAGPRTLGAHTGAATELGGAQCHWAERHRLFAVEHACLSNPCANGGTCVEEAEGFYCVCPEGWTGPSCTTGVNECQPNPCSQGGTCRQLQHGFECLCPPQWTGRTCQLDTNECDRGVCVHAVACHNLIGGYFCDCQPGWTGQDCDITNHTCQGKCQNGAVCEESLTGYRCQCPPGFTGSYCQTRASHCDSAPCLNGGHCAEEEETPQCHCPLGYSGKHCEEVLDLCNPNPCQQGAPCQSTEGGYLCACPQGYEGKECLSLKDTCHEPHCQGEPSGSTGSGSTLYVVMVVLLAMLVVGTTLCIYLLAQLRRRRKERQHAGQDEGINNQRECVTLIRNLGGPPPVPRCREEIELSLPPTPETKKPRRSSAKLDISNREREKLNRFHFSDSQEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBN6</accession>
    <name>W5NBN6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin maturation protease</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin aminopeptidase ACTMAP</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal N(alpha)-acetyl-L-cysteinyl-L-aspartyl-[protein] + H2O = N-terminal L-aspartyl-[protein] + N-acetyl-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:74579"/>
            <dbReference type="Rhea" id="RHEA-COMP:12669"/>
            <dbReference type="Rhea" id="RHEA-COMP:18395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64720"/>
            <dbReference type="ChEBI" id="CHEBI:78236"/>
            <dbReference type="ChEBI" id="CHEBI:193599"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:74580"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal N(alpha)-acetyl-L-cysteinyl-L-glutamyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + N-acetyl-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:74583"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:18396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:78236"/>
            <dbReference type="ChEBI" id="CHEBI:193601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:74584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + H2O = N-terminal L-aspartyl-[protein] + N-acetyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:74571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12669"/>
            <dbReference type="Rhea" id="RHEA-COMP:12693"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64720"/>
            <dbReference type="ChEBI" id="CHEBI:71670"/>
            <dbReference type="ChEBI" id="CHEBI:133063"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:74572"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + N-acetyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:74575"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:71670"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:74576"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ACTMAP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NBN6">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018045"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018077.1">
        <property type="protein sequence ID" value="ENSLOCP00000018045.1"/>
        <property type="gene ID" value="ENSLOCG00000014662.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQQD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012368"/>
    <dbReference type="HOGENOM" id="CLU_077492_1_0_1"/>
    <dbReference type="InParanoid" id="W5NBN6"/>
    <dbReference type="OMA" id="ANYEPCM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014662">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040043">
        <property type="entry name" value="ACTMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28631:SF1">
        <property type="entry name" value="ACTIN MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28631">
        <property type="entry name" value="UPF0692 PROTEIN C19ORF54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21646">
        <property type="entry name" value="ACTMAP-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="253" mass="27453" checksum="5058EDA9ABA7D2EF" modified="2014-04-16" version="1">MCSFCSFRRDLQWVLFNKYVPSLIQDGPQCGLVALWMAGHLLQRPTPLPLEQIVARALGRGYTAQGEMFSVPSTTLAAGPKAVKMSNSDSGGAVQRGHNTVHDTEQTXSSQATAAIITRSLCFSSWFTKPGVFRGLWCVLAGVLLGVARGTDTGQLQHDPQLPWLLLPRASGAPWLPRQGVEEVHLLAKQGKSLRYQLWALERLRDSNGQLCQLDPQRAQDGTRYVLPPGGVGAGLAGQVVLLHRRDQAAPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCY0</accession>
    <name>W5NCY0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCY0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018489"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018521.1">
        <property type="protein sequence ID" value="ENSLOCP00000018489.1"/>
        <property type="gene ID" value="ENSLOCG00000015020.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694020"/>
    <dbReference type="KEGG" id="loc:102694020"/>
    <dbReference type="eggNOG" id="ENOG502S61A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011425"/>
    <dbReference type="HOGENOM" id="CLU_075832_0_0_1"/>
    <dbReference type="InParanoid" id="W5NCY0"/>
    <dbReference type="OrthoDB" id="8778600at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015020">
        <property type="expression patterns" value="Expressed in liver and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034121">
        <property type="term" value="P:regulation of toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027869">
        <property type="entry name" value="TASL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14889:SF2">
        <property type="entry name" value="GENE 6377-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14889">
        <property type="entry name" value="RCG36411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15133">
        <property type="entry name" value="TASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="319" mass="34486" checksum="656AD69896F39FF0" modified="2014-04-16" version="1">MLAEGFLRAIEYREDLSATAAACPRRPRPRPSKKSHQDSWEDLHGDFVILGAVREDTPSPEQDPRQSPKQTPPLGVPRTGGASSALGSAADGGPWGLELYRSWGCTSICKDYPDLQIGGDHVGDRGSPPSGPIGLGPNCEACLGPLLQSGDLEALETLEPLPLRSGPQLPDWGAQERASGHGDTLADEGYLAMDSSISLNREPLSNSMLNTYLETKLLEVYKQYLQDRLAKCASPSRALPSSFVQYNVQQLSLQLSQEQGLDAHRARSIVINYLSTRSSSSGQISSPVLRISNMEPRKFPEPAKKKPPPLVTGVLHTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDE9</accession>
    <name>W5NDE9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 87</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDE9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018690.1">
        <property type="protein sequence ID" value="ENSLOCP00000018658.1"/>
        <property type="gene ID" value="ENSLOCG00000015160.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQN1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018647"/>
    <dbReference type="HOGENOM" id="CLU_016540_0_0_1"/>
    <dbReference type="InParanoid" id="W5NDE9"/>
    <dbReference type="OMA" id="GEWDWNT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015160">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037383">
        <property type="entry name" value="CCDC87"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16078">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16078:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="647"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="84960" checksum="35B6E0E5FFEA62D2" modified="2014-04-16" version="1">TPIRDSVKVSPASLEELCQLVHRRVQVRPEITAVSEEAQRTLAGVILAEVKLIWQDLKNLTCDPSLSSEENEALRCQMTTRIILVCEQLFLHYLCMVDTLRRRCVFSDQANLSRLGAQLSVECTRLIGTCPLSTSIICGYISMYETIIFHGSCGTGRRTHESVVERDLREIHEAIAPLDLEKVYELLPFQEEQVYARKAMQCKNFWMLIKSWCLTQRDAHVVLLKGYHSMPDLYRESLLEELGIKPERARPNSPVVLHTSVLAASLEKPVSISDDDGNKSPLQQSKADDPEADLPPLIRAVICRKEAKWLHVQETLKKLNEEEEQTPVEQFVVKEPEHPQAAVVNVTLSSQCVARPADVQVSDRVLTDTVNIRKCPPVYNDLTGEIEASTVKWLDRNLFLGQEIKDVYKELTKSVSTEYLDFDQDPLIERAAADADMPIVLASSALNRKRRHWFINPQLREPNRNNLMQRENKKNNTTNDYERPDNVSSRAYASWLQWWKSTLNFDDYLKYISTQDSDYLKVIFHLYNSDESDDEEDERRKALVSEMEYREREQRKKIAALRSQKQEFVSGLWNVNSVMLGGLGKEPELGAQKCFLQAERSQAARSGSAGTEEELLQSRLERIWEALYVPDGERLDMAIKYSSHTYRERLEEATTAWERAVQFIQQRESLLAKLERFERNASDPNRFFERGYRGTSIARLDESKRRGKLHSEISAVESLLSKAVHRVKDRFNDTVTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEK6</accession>
    <name>W5NEK6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 120 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the constitutive coactivator of PPAR-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027912">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEK6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019065"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019097.1">
        <property type="protein sequence ID" value="ENSLOCP00000019065.1"/>
        <property type="gene ID" value="ENSLOCG00000015490.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRMW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063168"/>
    <dbReference type="HOGENOM" id="CLU_007639_0_0_1"/>
    <dbReference type="InParanoid" id="W5NEK6"/>
    <dbReference type="OMA" id="MPWEVFD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015490">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18672">
        <property type="entry name" value="PIN_FAM120B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026784">
        <property type="entry name" value="Coact_PPARg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976:SF17">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="784" mass="86715" checksum="0D594F4173494358" modified="2014-04-16" version="1">SSGPFPVCSSKSFLVAVPAMGVKGLQGFVENACPGTCVAVNLKEMAEQRRRARPGRPPTLVVDGTACLRHWYSCAAWVHGGQWREYTRVLESFAGAFAEAGIRLVFVFDGAVEEAKRAEWAQRRLRHGREIRGAFRYVKAHGRQPGREMFCLPSGLATFSRFALKALGAETRCSVREADYEIAGYAALHDCMGILGQDTDFLIYDSVPYLSVSQLRLERMETVLFSRENLCRALRLRQSDLPLLACILGNDVVPQGRLEGLRNKCLAAYRQKHPGASTPADKVLAVADFISSHCCSRGGISELPLSEADQAVLEQGVQMYLLPNQQSPWVPSGSEPPSSESMNSLERYSSHDVLQVTLQGAALEKISATEPLNVDMPVPSSCLQNLSHICACIPLMGQLLNSPAATFATAVLVSAERRRMIIVTFQLSCKKTVTNLQPDSSLYGNPTKHPFLYVDENKYCNLGTILRKILPLWLLVLLIHLEVPKMHQMPFWNQPYSFMLVSLFVFKAAKEQHVRAESFMVFDVLSAGVVDCSNTLEDEEDTEFPGQAITFRPARERIYGILFAVKQGDSGPAPAVKEWFVYRGNSLRQPDLVTPAPLSLPEGTPDLKVLWFSEAPETKALRFSTFLAVFDLLDLAEELEKLESPLAAVVCLVTYIALQERSLCLEDVNAYVSQAVCVREKTDEELAGIKLPVVDPRAIELGSLFVRGLTILIAANSASGFPLKMEDLMPWKVFDGLLFHSKYLQAHSGCSEDDLLEGTECWVSQFGHLKALVTSACGRRNRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFP0</accession>
    <name>W5NFP0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
            <location evidence="1 9">Extracellular space</location>
            <location evidence="1 9">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633437.1">
        <property type="nucleotide sequence ID" value="XM_006633374.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFP0"/>
    <dbReference type="FunCoup" id="W5NFP0">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019449"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019481.1">
        <property type="protein sequence ID" value="ENSLOCP00000019449.1"/>
        <property type="gene ID" value="ENSLOCG00000015799.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687765"/>
    <dbReference type="KEGG" id="loc:102687765"/>
    <dbReference type="CTD" id="51384"/>
    <dbReference type="eggNOG" id="KOG3913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157480"/>
    <dbReference type="HOGENOM" id="CLU_033039_1_0_1"/>
    <dbReference type="InParanoid" id="W5NFP0"/>
    <dbReference type="OMA" id="TCWLQMP"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015799">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060349">
        <property type="term" value="P:bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990523">
        <property type="term" value="P:bone regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072498">
        <property type="term" value="P:embryonic skeletal joint development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030500">
        <property type="term" value="P:regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902036">
        <property type="term" value="P:regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19344">
        <property type="entry name" value="Wnt_Wnt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013304">
        <property type="entry name" value="Wnt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF70">
        <property type="entry name" value="PROTEIN WNT-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01895">
        <property type="entry name" value="WNT16PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="352" mass="39771" checksum="481AAFD090E90659" modified="2014-04-16" version="1">MEKSAKFGIHRLCIFLILFFSFFPFHSQGSWMWLGITSLSIPEKMGCGNLPLTNKQKDLCKRKPYLLPSIKEGARMGISECQTQFKYERWNCSTTSEFSVFGYELTSGTKETAFIYAVMAAGLVHTVTRSCSAGNMTECSCDTTLQGGGSPREGWHWGGCSDDIQYGMWFSRRFLDSATGNASALLAMNQHNSEAGRQAVAKMMSTDCRCHGVSGSCAVKTCWKTMSTFEKVGSFLKDRYENSIQVLDRSRKKVRRKEKDQRRVPVSKEELIYVNKSPNYCLEDRKAGIAGTRGRKCNRTSAGPDGCNLLCCGRGYNTHVVRHVERCECKFVWCCYVRCRRCESMTDMHTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFR6</accession>
    <name>W5NFR6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">AP3A hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Ectonucleotide pyrophosphatase/phosphodiesterase family member 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. Acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01008314">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008315">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFR6"/>
    <dbReference type="FunCoup" id="W5NFR6">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019475"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019507.1">
        <property type="protein sequence ID" value="ENSLOCP00000019475.1"/>
        <property type="gene ID" value="ENSLOCG00000015820.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158831"/>
    <dbReference type="HOGENOM" id="CLU_017594_1_2_1"/>
    <dbReference type="InParanoid" id="W5NFR6"/>
    <dbReference type="OMA" id="DVCIDHS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015820">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000004">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF79">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE ENPP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867699" description="bis(5'-adenosyl)-triphosphatase" evidence="22">
        <location>
            <begin position="20"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="531" mass="60168" checksum="81CE0D85793F076A" modified="2014-04-16" version="1">MLVGLFLCVLAVEVGLGVCEDTGNMTALQNTSGPPLLLVSFDGFRADYLKKFNFPNLQKFFFSDGVVVEQLTNVFVTKTFPNHYSIVTGLYAESHGMLASTMYDPVTKKNFSIANDSDPFWWNEATPIWVSAQKQGYKTAAAMWPGTDVPIHNMTSTYYLRYDSKVTFQERVGNLTKRLTEDETVKFAVLYWEEPDHAGHLYGPDNTTEMGKVLKDVDNLIGFLVDKLNQSGLWGKMNVIITSDHGMTQCSKDRLIHLDKCIDSANYTSVYLTPVAAIIPLTNATQVYNALKDCHPNMKAYLQAEIPDRFHYRDNERIQPIILVADEGWTIVQEESHLRLGDHGYDNALPSMHPFLTAHGPAFKKGYRMSSLNIVDIYPLMCHLLHMEKMPNNGSFYNSHCLLVDERCTDLPEVIGIVIGVLMVLTTLTGLFVLMKNRTPSSSRPFARLELQDDDDDDPLLGDSSKILQWTSRWNQICITFLADETDRIALWGKNCCWLLGSYLFFAKETRSWIVLGAMKKNLTKSYLFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG38</accession>
    <name>W5NG38_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01001612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG38"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019597"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019629.1">
        <property type="protein sequence ID" value="ENSLOCP00000019597.1"/>
        <property type="gene ID" value="ENSLOCG00000015909.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1033">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160736"/>
    <dbReference type="InParanoid" id="W5NG38"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015909">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19903">
        <property type="entry name" value="DSRM_EIF2AK2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000251">
        <property type="entry name" value="eukaryotic translation initiation factor 2-alpha kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000548">
        <property type="entry name" value="Z-DNA binding protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044452">
        <property type="entry name" value="EIF2AK2_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF194">
        <property type="entry name" value="DOUBLE-STRANDED RNA ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DRBM" evidence="11">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="11">
        <location>
            <begin position="100"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="404"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="690" mass="78659" checksum="21FE5CD3A81CEE47" modified="2014-04-16" version="1">MSQMNYVGRLNEYRQKHNLSLQYVEVSAEGPDHVRQFTMKVVLDSQAYPDGVGMSKREAKQNAAKNALEKLWVEVSQQNDPVNSSRESSCPSPVSTQGINHLCWLNEYGQKKGVLVQPFERSDGGPPHMPQFSCRFAIGNRNFPEAFGKNKKDAKKEAAKLAYEALQEKSTPEEEDSIIAQRAPQRAGRALRVSNIFLSLRYIQRGSAPEVSPVTGCLSNFCQPTWSCVFGKETEQGAGKVYLSRCALDSLKRFSLSHSHLPVQHHQALARHHLLHTWGKSYRCHRIKEQLTSAQTFLFIFNGIPHDTSILTTPERLNRDDESDYIIFRDSSDKKLPTGEFIGIFTSPIAIFHCLLLLPLCLLWPASVKKVKDANFTVISSHSFAPSFQMLHTCVIRVKFLQDFDLIETIDKGGFGSVFKARRKLEGKHYAVKIVKHTPKAEREVGALAGLQHQYIVRYFSSWLEDTMYQAEKTDSSSSSSSGPGTPKEFLYIQMELCEGGTLRSWIDQEQDGRPKDKEKTMSIFRQIVEGVKYIHSKQLIHRDLKPPNILFSHERTVKIGDFGLATSAINESDSNSIQRTKRTGTVTYMSPEQETQNQYGEKVDIFPLGLIFFELLWRFKTKTERQKTWHSIRNREFPSEFCKQHHNEHKLIEQMLSQEPEERPEASKIAKNLKVLLKSASELQDSKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGW1</accession>
    <name>W5NGW1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">tRNA pseudouridine 55 synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:57877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:42533"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01001657">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGW1"/>
    <dbReference type="SMR" id="W5NGW1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019904.1">
        <property type="protein sequence ID" value="ENSLOCP00000019870.1"/>
        <property type="gene ID" value="ENSLOCG00000016123.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007529"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016123">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2050:FF:000001">
        <property type="entry name" value="putative tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048742">
        <property type="entry name" value="Pus10_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21237">
        <property type="entry name" value="Pus10_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pus10 N-terminal eukaryotes" evidence="20">
        <location>
            <begin position="109"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Pus10-like C-terminal" evidence="21">
        <location>
            <begin position="289"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="535" mass="60626" checksum="47BEE3510C41E8EE" modified="2014-04-16" version="1">MLPLKEKDRTIVQTLLKAGCCPRCVLRFCCVGLQSAYRQPYQILEDELHRFAKPDESSNPTAAAEEEEDPPCKRIKLEGAEISVAGEKGTAIPLPGDSFDTQKNEQNVCVVCLGVLQDFCDKDFAQKMSRAVDSAGYQFNSLVVSVSLPPQLSVREHSVWLLVKKEVGLKGLCLGKDDVIQIKEAYKWLIHPLISQELGVPVNGKSSFEVSIAFIHPETDGDCHFLAAACSDCFKPAKNKQSVFTRMAVIKALEKIKDADFRKQFPCPPCPPVTKCVAQDIQCLHSPVFIAGRYNKYSRTLPQTPWVIDGERKMESSVEELISDHLLATFRADGFNFSSSGREDVDVRTLGKGRPFAVELLNPHRAVFTKDEIKKLQETINNSSSEKIKVRDLQIVTRDAVGHMKEGEEEKTKSYRALIWTERPIQEHDIEFINDIKELRVNQKTPLRVLHRRPLAVRQRVIHTMKAQFLDEHHFQLHLRTQALSYIKEFVHGDFGRTKPNLCNLMKTVTDILELDVESVDVDWPPAIEDEDTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHA9</accession>
    <name>W5NHA9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Leucine rich repeat containing G protein-coupled receptor 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHA9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020018"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020051.1">
        <property type="protein sequence ID" value="ENSLOCP00000020018.1"/>
        <property type="gene ID" value="ENSLOCG00000016234.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694225"/>
    <dbReference type="KEGG" id="loc:102694225"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160214"/>
    <dbReference type="HOGENOM" id="CLU_006843_0_0_1"/>
    <dbReference type="InParanoid" id="W5NHA9"/>
    <dbReference type="OMA" id="PSSMGFM"/>
    <dbReference type="OrthoDB" id="1883493at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016234">
        <property type="expression patterns" value="Expressed in larva and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000028">
        <property type="entry name" value="leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF71">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867763" evidence="19">
        <location>
            <begin position="25"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="637"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="766"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="801"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="572"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="905" mass="100000" checksum="C47CE6FE92FFA9D5" modified="2014-04-16" version="1">MLHPCTVLVIVKLLLLVADCGGTAQTGARHSDCPSRCHCEEDGMLLRVDCSDLGLTALPANLSVFTSYLDLSMNNITQLPASALSNLHFLEELRLAGNDLMHIPKGAFSGLFNLKVLMLQNNQLRQVPSEALQNLRNLQSLRLDANHIYSVPHNCFDGLVGLRHLWLDDNSLTEVPIEALSSLSTLQAMTLALNEITHIPDLAFANLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPVAIKALTNLKELGFHSNNIRSIPEHAFIGNPSLITIFFYDNPIQFVGKSAFQHLPELRTLSLNGATEITEFPDLTGTKSLESLTITGAQITSLPSAVCEQLPNLQVLDLSYNLIQHLPSFNGCKKIQRIDLHHNEIHEVKANTFQELTALRSLDLAWNKLHAVDPNAFSNLPSLIKLDLTSNLLSSLPVTGLHSLTHLKLAGNEGLQELLSADNFPKLRVMEMPYAFQCCAFVSCENHNKPTNLWDKEENSSRDELGKKETSIMGHQEDQDLEDFLLDLEEDPKAQHSVQCSPAPGPFRPCDHLFGSWLIRSGVWIIVVLSLVCNGLVAVIIFFSPTYVSPAKHLIGLLAVVNSLMGLSSGILAVVDALTFGRFAKYGAWWESGIGCKITGFMSVFASETCIFLLTTAALERGVSIKCSKRVEIKSSTNNVRIVSAFCFVLALAITVLPLLHVGEYGISSLCLPLPFGEPSSLGFMVALVLLNSLCFFVMTVTYTRLYCNLEKGELDNFWDCSMIKHVAWLLFTNCILYFPVAFLSFSSLLNLSFISPEVIKSILLVIVPLPACLNPLLYMLFNPHFKEDLSLLLKKTRLLKRSKQASLVSLNSEDAEKQSCDSTQALVTFISSEKPGNISESKSISPSHQKVEGYHSQSVTFVPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI45</accession>
    <name>W5NI45_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 117</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI45"/>
    <dbReference type="FunCoup" id="W5NI45">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020304"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020338.1">
        <property type="protein sequence ID" value="ENSLOCP00000020304.1"/>
        <property type="gene ID" value="ENSLOCG00000016439.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QXR1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013052"/>
    <dbReference type="HOGENOM" id="CLU_047657_0_0_1"/>
    <dbReference type="InParanoid" id="W5NI45"/>
    <dbReference type="OMA" id="NWEWRST"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016439">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="TMEM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31226">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31226:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15113">
        <property type="entry name" value="TMEM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="293"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="404" mass="46228" checksum="1EA790A8D7C87A83" modified="2014-04-16" version="1">MTFTSADCLSFSLCSPHIVTEYMGIRNESFMKLAAVGTWMGDFVTAWMVTDMMLQDEPYPDWGKTARRFWKKGNHRIVLFWTVLISLTSVVVVVISTDWISWDNLNRGFLPSDEVSRAFLASFILVFDLLIVMQDWEFPHFMGDLDMNLPGMSTTQVKLKLPVCKSIFKEEYHIHITGKWFNYGIICLVLILDLNMWKNQIFYKPYEYGQYVGPGEKIYTVEDPDTLSEFNKSTLTWEWRSTHIDPTYNETYSRGDMFLHSRYVGASLDVKCLAFIPSLAAFFLLGFFIWLFGRFQDSEQGPKNKDKTYERIKKKSPSEYSKDMGVTPEEPHVSMNEALKSNTPMLLSVDAPNVQSNGSASYSSTETQEIHPNIVIDSLAPTESAVSIDVYKPSPCQEGQTALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKB4</accession>
    <name>W5NKB4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Adhesion G protein-coupled receptor B3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Brain-specific angiogenesis inhibitor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor that plays a role in the regulation of synaptogenesis and dendritic spine formation at least partly via interaction with ELMO1 and RAC1 activity. Promotes myoblast fusion through ELMO/DOCK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021073"/>
    <dbReference type="MEROPS" id="P02.027"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021109.1">
        <property type="protein sequence ID" value="ENSLOCP00000021073.1"/>
        <property type="gene ID" value="ENSLOCG00000017045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689136"/>
    <dbReference type="KEGG" id="loc:102689136"/>
    <dbReference type="CTD" id="577"/>
    <dbReference type="eggNOG" id="ENOG502QRKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155081"/>
    <dbReference type="HOGENOM" id="CLU_003751_1_0_1"/>
    <dbReference type="InParanoid" id="W5NKB4"/>
    <dbReference type="OMA" id="CESKNAF"/>
    <dbReference type="OrthoDB" id="5989160at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017045">
        <property type="expression patterns" value="Expressed in brain and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15989">
        <property type="entry name" value="7tmB2_BAI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000003">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000012">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
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    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043838">
        <property type="entry name" value="AGRB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008077">
        <property type="entry name" value="GPCR_2_brain_angio_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF40">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19188">
        <property type="entry name" value="AGRB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01694">
        <property type="entry name" value="BAIPRECURSOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869874" description="Adhesion G protein-coupled receptor B3" evidence="19">
        <location>
            <begin position="26"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="876"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="910"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1018"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1096"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1123"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="21">
        <location>
            <begin position="499"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="20">
        <location>
            <begin position="691"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="22">
        <location>
            <begin position="874"/>
            <end position="1148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1515" mass="170043" checksum="AE006D7A405B68F3" modified="2014-04-16" version="1">MKAIRNLLIYIFSTYLLVMFGLTGAQDFWCSTLVKGVIYGSYSVNEMFPKNFTNCTWTLENPDPTKYSIYLKFYKRDMSCSNYALLAYQFDHYSHEKINDLLRSNESIVYLCDSKNIYIFLQYDKNFVQLRRVYPFDYPGVTHKRAEDQRSELEFLVLNKVSPSQFGCQVLCSWLENCLKAEKGTVESCGIVYTKCSCPQHLGDGESENALVLNNVVLPLNAQTEGCLTPHLRTAQVCNLSAEVKRPSKEEFGMIGEHTIKSQRPRSVHETQVPQEQADSAKFMAQTGVSGAEEWSQWSSCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCIPPQYGGRACDGPETQTKLCNIALCPVDGQWQEWSQWTECSVTCSNGTQQRSRQCSAAAHGGSECRGHWAESRECANPDCTANGQWNPWGHWSGCSKSCDGGWQRRSRVCQGAAITGQQCEGTGEEVRRCSEQRCPAPYEICPEDYAASMVWRRTPSGELAFNRCPSNATGTTSRRCSLDHRGVAFWEQPSYARCITNEYRSLQQSIKGYLAKGQRMLAGDGMSQVTKTLLDLTQRRNFYAGDLLSSVEILRNVTDTFKRANYVPSSDDVQNFFQIISNLLEEENKEKWEDAQQIYPVSVELMQVIEDFIHIVGLGMKDFHNSYLMTGNLVASIQKLPAVSVLTDINFPMKGRKGMVDWARNSEDKVVIPKGIFIPQSEELDGSTVFILGTVLYKNLGLILPSPRNYTVVNSKIIAVTVRPEPKIAESHLEIELAHLANGSMNPYCALWDSSMINDSWGSWSTKGCKTVLTDASHTKCLCDRVSTFAILAQQPREITMEYSGVPSVTLIVGCGLSCMALITLAVVYAVLWRYIRSERSIILINFCLSIICSNILILVGQTQTHNMGVCTMTTAFLHFFFLASFCWVLTEAWQSYMAVTGKVRTRLIRKRFLCLGWGLPALVVAISMGFTKAKGYGTPQYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRTGQMSEPHTGLALKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINVDTTGTFPNGHAQIMTDFEKDVDIACRSALHKDMGSCRAATITGTLSRISLNDEEEKGPEAMSYSTLPGNIMSKVMMKQPSGLHMPIGMSELSDQCINDNNSDMRRTVYLCTDDNLRAGDTEMLHPQGQMLESDYIVMPRGSGNVQPHMKDESKLNIGMDSLQHERLMHYKVNPDFNINPPGMDHINVNLDQQFAAQEHMQNIQFEPRTAVKNFLAEIEENTGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNMASMDKPTPNKHPWESYNAPCEYQNYATMNVLETDAKDSLEMTPAEWEKCVSLPLDVQEGDFQTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLA4</accession>
    <name>W5NLA4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NLA4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021450.1">
        <property type="protein sequence ID" value="ENSLOCP00000021413.1"/>
        <property type="gene ID" value="ENSLOCG00000017308.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008372"/>
    <dbReference type="HOGENOM" id="CLU_1063697_0_0_1"/>
    <dbReference type="InParanoid" id="W5NLA4"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF107">
        <property type="entry name" value="HYDROXYCARBOXYLIC ACID RECEPTOR 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="52"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="278" mass="31449" checksum="B2A4479C4D924D49" modified="2014-04-16" version="1">MWVFHTNISTILNSSTLNVTDTHCESTDLHRFPAMLWLKAAEVITAILGLPANAKVLWIILKNKADSSTSDIFTANLALMDILFCSMIALEILNTHFLNQPNISQGLYFFMGLSEIGGPFFLTCVCVDLYLAVLHPIAFMKFRDHKWRAVCSAVAWVFTVCYSTSLMIIQMDYMEVILSVIQFSILIVIVICNIYILRALKESGPGGQEVHPIKKRAYNTVLVNFLIIMISYIPAVLAFTLKSRFTNHVFSCYIIPFPLSFMCVSNCVQPFLYIYRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLL3</accession>
    <name>W5NLL3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin-19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in endosomal trafficking of the epidermal growth factor receptor (EGFR).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with EGFR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the syntaxin family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217154.1">
        <property type="nucleotide sequence ID" value="XM_015361668.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217156.1">
        <property type="nucleotide sequence ID" value="XM_015361670.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLL3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021522"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021559.1">
        <property type="protein sequence ID" value="ENSLOCP00000021522.1"/>
        <property type="gene ID" value="ENSLOCG00000017417.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="W5NLL3"/>
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    <dbReference type="OrthoDB" id="10255013at2759"/>
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        <property type="component" value="Linkage group LG14"/>
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        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:SNARE complex"/>
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        <property type="term" value="F:SNAP receptor activity"/>
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    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE"/>
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    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
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    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
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        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SYNTAXIN-19"/>
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    </dbReference>
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        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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            <begin position="209"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
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    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="195"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062006"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00069802"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="292" mass="34096" checksum="C476FC47CE0D9EDE" modified="2014-04-16" version="1">MKDRLEELRQRAKDAEMEKEKNTFADDVTDGASFSPQAVVFETEPGIENFLSEVQRIRDSINDLADEVKRFSQQQKNLVATMRRFSVMKKESSVTRDIKLQAESLHRKLDALAKRVKSTEAEHGPNAAITRIQSTQYSTLFRHFQQVMRQYNDTLVSKQDKCKQFIVLQLEVAGKEVSEDEVDEMVEQGKWEVFNENVLNEVKITKAQLTEIEQRHKELMNLESNMKDLRDLFLEIYLQVEEQGEHIENIESNIQKTQDFVQKTNEKFKLAAKYRKKNPLKKMCCCCCPCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLM0</accession>
    <name>W5NLM0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-glycan biosynthesis class W protein</fullName>
            <ecNumber evidence="5">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01005672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLM0"/>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021529"/>
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        <property type="protein sequence ID" value="ENSLOCP00000021529.1"/>
        <property type="gene ID" value="ENSLOCG00000017424.1"/>
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    <dbReference type="OMA" id="GLYVMQP"/>
    <dbReference type="UniPathway" id="UPA00196"/>
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        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017424">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="255"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="503" mass="55808" checksum="0B17798DF5880562" modified="2014-04-16" version="1">MSQKEIKEAFVSNLNGTTLGEISLGSALAPLCLLSRGLGLILYFLGKGVFPFPWKVHLLLDFTVVVLPLILSCTVLSDALHVVIVSLAAFDASMLFRVYHRRKHSPAVPFRRTFKDFLGSSLETDRVPFVTVFRVLVNVKTAISILAVDFAIFPRRYAKSETYGTGVMDFGVGAFILANSLVCPEARRKQPPPSKFAHLTKQLLSVWPLVALGLARLLSVKATGYHEHVSEYGVHWNFFFTLAIVRAVASLLLSVFPVNRSWLLAVIIGGSYQAALELTDLKSFILNGSDRTGGFVSANREGVFSVIGYVAIYMAGVQVGLHVMESRMVVKDWIKAIARLLLGSFALWAVLFVCQMFVEPVSRRMANLSFGIWTVAQSLYFLCCLSAADLVLAFAKFLSNCRSVSSSWSPSQAPQTQNSDSKSSSVDRKIREDIEGLCLIQAVNRNQLLFFMLSNIMTGLTNVMVDTIHSSSVFSVFVLVFYMFANSFIVYVLHLQRITVKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMC9</accession>
    <name>W5NMC9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U6 snRNA-associated Sm-like protein LSm6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA. Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome. Component of the heptameric LSM1-LSM7 complex, which consists of LSM1, LSM2, LSM3, LSM4, LSM5, LSM6 and LSM7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMC9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021788"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021825.1">
        <property type="protein sequence ID" value="ENSLOCP00000021788.1"/>
        <property type="gene ID" value="ENSLOCG00000017683.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154978"/>
    <dbReference type="HOGENOM" id="CLU_076902_7_4_1"/>
    <dbReference type="InParanoid" id="W5NMC9"/>
    <dbReference type="OMA" id="MYISEQK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017683">
        <property type="expression patterns" value="Expressed in ovary and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120115">
        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000010">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9 16" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="17">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="80" mass="9036" checksum="57B151B8F461B4F5" modified="2014-04-16" version="1">MSLRKQTPSDFLKQIIGRPVVVKLNSGVDNRGVLACLDGYMNIAVEQTEEYVNGQLKNKYGDAFLRGNNVLYISTQKRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NME1</accession>
    <name>W5NME1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NME1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021837.1">
        <property type="protein sequence ID" value="ENSLOCP00000021800.1"/>
        <property type="gene ID" value="ENSLOCG00000017695.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_045922_2_0_1"/>
    <dbReference type="InParanoid" id="W5NME1"/>
    <dbReference type="OMA" id="PRREEWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="146" mass="16082" checksum="47B1943D4FB6E34B" modified="2025-10-08" version="2">MIVHMFNPFVQEAEIVIFFKRFVDIKGGGVKLIDSRNYWSGKRRFMVRLKTNKSVEGGFIHPPALFSIGSNRGFLVYPGQSRVGRNCAARGHVAAECTVICCRRCCGLGHKAGQCSKPLTCNLCGEAGHLYSICPQRPRSYAGAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN31</accession>
    <name>W5NN31_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G0/G1 switch 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01016729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_015197911.1">
        <property type="nucleotide sequence ID" value="XM_015342425.2"/>
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    <dbReference type="FunCoup" id="W5NN31">
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    <dbReference type="STRING" id="7918.ENSLOCP00000022040"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022079.1">
        <property type="protein sequence ID" value="ENSLOCP00000022040.1"/>
        <property type="gene ID" value="ENSLOCG00000017937.1"/>
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        <property type="entry name" value="G0_G1 SWITCH PROTEIN 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15103">
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="111" mass="12634" checksum="DFFAA7422E4FF480" modified="2014-04-16" version="1">METMQELIPFAKEMLSQKPSRKMVRVYLVGSVLAFFGVVIGLVETVCQPFSSDAGVDEEIQRLAERERRALEAEERREMEEIATSENDRVVKPKRQVITGQRNAANRLHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNE2</accession>
    <name>W5NNE2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Actin like 7A</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the actin family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNE2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022151"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022192.1">
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        <property type="gene ID" value="ENSLOCG00000018050.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162158"/>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="W5NNE2"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="404" mass="45004" checksum="E5BF897BBD9653FC" modified="2014-04-16" version="1">TKGPAKVAKLIKINSNEGPSAPDKHPIPKSQQRVVVKRTKAVIIDLGTGYLKAGFAGQPGPSCVIPSAEGVTRRRSDVAGFSRRESFVGKELLQRPDLEWTAPLQHGIVTDWEAVERLWEYVFQQELQVPFEEHAVLVSDPPLSPTTNREKFAELMFETFSIPAIHIAYQSVLSVYSYGRTTGIVLESGHGCSYAVPIHDGYHMPSHTGRVDYGGQSLDAYLLSMLQDSGKTIPDNLHSIVEDIKKKCCYISLDTDHEMSLDKNDCAVDYELPDGHLISIGKERFACPEALFQPIALGSREPGLQTMVMNCINKCDIQIKKDLLKNIVVCGGSTMFPGFPERLQYELDKIAPKSEAAVVAVPERNYAVWRGGSILASLQAFQSLWVRKEEYEERGPFIIYRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP87</accession>
    <name>W5NP87_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Family with sequence similarity 237 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP87"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InParanoid" id="W5NP87"/>
    <dbReference type="OMA" id="NWHQTET"/>
    <dbReference type="Proteomes" id="UP000018468">
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    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040439">
        <property type="entry name" value="FAM237A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36690">
        <property type="entry name" value="PROTEIN FAM237A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36690:SF2">
        <property type="entry name" value="PROTEIN FAM237A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="87" mass="10084" checksum="33966721AD6E224F" modified="2014-04-16" version="1">PVILGTIVPECWDSSSIAVMEIKKLKVGDTVSDFWDFMIYLKLSDYPKNNTLFEDLAQLFWNIYVDCTLSRAHGLGRRYIISKKYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPB1</accession>
    <name>W5NPB1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPB1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022511.1">
        <property type="protein sequence ID" value="ENSLOCP00000022470.1"/>
        <property type="gene ID" value="ENSLOCG00000018369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161337"/>
    <dbReference type="HOGENOM" id="CLU_077059_0_0_1"/>
    <dbReference type="InParanoid" id="W5NPB1"/>
    <dbReference type="OMA" id="WTYILIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000096">
        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="35090" checksum="CFFED391F15214D8" modified="2014-04-16" version="1">VTSGNILTVKDDYLPILPLTIVKLFILVFTYIGTNFVNGMLIFTYFYHPIFYENSRYVLFVHLVINDVFELSVAMTLHIWLRVKPSVFVPLCYTLVILASNTTLNTPLNLALMSLERYIAICYPLRHSQICSLQRTYIAIGVIWIVGIIPSITDLFIVLAVEQLSFFSTSVYCIRESFYRTSYQTKIKTAIMSVYLTVVWLVIVCTYLRIMFAARSASSSEKTSAKKARNTILLHGLQLLLAMLTYIPPFTDPFLVGISTEIFLDLSFSRYYLVYIVPRVLSPVIYGLRDENFRKHLKSHMPCFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVU9</accession>
    <name>W5LVU9_LEPOC</name>
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        <submittedName>
            <fullName evidence="14">Zinc finger and BTB domain containing 37</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVU9"/>
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        <property type="gene ID" value="ENSLOCG00000000232.1"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159554"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
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        </location>
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            <end position="421"/>
        </location>
    </feature>
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            <end position="450"/>
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            <begin position="142"/>
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        </location>
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            <begin position="488"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="498" mass="54905" checksum="E601E2FBC0AE084D" modified="2014-04-16" version="1">PSMERAGSIQLEIPDFSNSVLSHLNQLRMQGRLCDIIVNVQGQSFRAHKVVLAASSPYFRDHMSLSEMSTVSLSVIRNPTVFEQLLSFCYTGRLCLQLADIISYLTAASFLQMQHIIDKCTQILESIHFKISVADVEAELSQGARGKHQDRSLESSRGGAPRSLSPRHGSPRGSRRSGGGVIDVSPQIIEHGASEGLGKEPILRINRAGQWYVETGLEAGRGGALDEDVRVVDGLRIKTERLEEWMAAETQASGEEGSSAEEVTAMMIDTTGHSSLPQEGFSMGASGAKGSQPSSSFSETDRFSPTGSVVVVAERQRAKSESPWRVDDQRQPTSQGEEQTVFDVGGYEEYLREQVGDRWFRYNPRLTCIYCCKSFNQKGSLDRHMRLHMGITPFVCRICGKKYTRKDQLEYHIRKHTGNKPFHCHVCGKSFPFQAILNQHFRKNHPGCAPQEVSHSASPETTVPSRGQNEDESPSQEEGGAAAFSEAVQPSVSTTGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW31</accession>
    <name>W5LW31_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW31"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000338.1">
        <property type="protein sequence ID" value="ENSLOCP00000000338.1"/>
        <property type="gene ID" value="ENSLOCG00000000312.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1668823_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW31"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000312">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="16130" checksum="EA3F2027BF4C6928" modified="2014-04-16" version="1">MLGESWGPTRPAPQGQEWRTQTAAPPRSTTTSRGFWEQRRELVHAGPVSGPVSCPVSGPVSGPVRCPVSGPVLGPVTCPVPGPVLGPISGPLSGPVLVPISVLVTDTVLGPLSGPVLVQLQAYTCIATHALPVCVCVCVCEGAASELRRSVGVSVRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXK1</accession>
    <name>W5LXK1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FISNA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LXK1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000858"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000862.1">
        <property type="protein sequence ID" value="ENSLOCP00000000858.1"/>
        <property type="gene ID" value="ENSLOCG00000000773.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SBIG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000253760"/>
    <dbReference type="HOGENOM" id="CLU_002274_3_0_1"/>
    <dbReference type="InParanoid" id="W5LXK1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000773">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="FISNA" evidence="5">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="828" mass="92279" checksum="5D29294E7892F76F" modified="2025-10-08" version="2">MQTLKDKHKEALKRKFEVVAEGIAKPGEQTLLNDVFTHVWITEGSCVEVNHGHEVMQVETVSKITKSEICSIKCNDIFKPLPGQTRPIRNVLMKGVAGIGKTFSVQKFILDWATGKDNQDFDFIFFLPFCKLNSIKSEMSLQELVQCFCPEIKSSANILDCCKVLFIFDGLDGSRHPLNFTRNQIWSDVKKPTSVDILIINLINGNFPVDASVWITSRPAATGLIPSELISRVTEVQGFEDKQKEEYIRKKCKSTDLADRIISHLKMLGSLYMMCYLPVFCWIVVTVLDHTLEENSGRRGKSDSTQPDSLSKLAKILQSEVSKQSLSTYSVSVPQEMDAACSLICIRRTQASFEPLGQLNIIAFRSECCSCGGLSVFHTRIGLGLNVIFRKEKICFLKPIESVILFLVQSSDAGVSVAHTLSNNITIYIRILYTLSTMKSNCINVNLVSYLHQQTALSQSHLATPPADIHVRFYLHCLSVSFKQCIVGELCQQEENSAQCTFCWAAGLKTSMNTISGEGSVEEYLCVSPCFPPSTFLTISSHLSFSSCIPRFETAVVFIILIHGLSEKDNDMMLYITVELGAVKDVDELCRCSVCRMSWCELTERCCEDLASVVQFQHSSLRELDLSFNNLGDSGVKLLSAALRDPNCKLTTLKILRCELTERCCEDLASALQSQHSSLRELSLSLNNLGDSGVKLLSAALRDPSCKLTTLRMVRCGLTERCCEDLASALQSQLSSLRELDLSYNNLRDSGVKLLSAALRDPNCKLTTLKMEMCRLTERCCEDLASVLQSQHSSLRELDLSRNNLWDLGVNLLSAALRDPSCKLTTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXP7</accession>
    <name>W5LXP7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Major histocompatibility complex class I UBA</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LXP7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000904"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000908.1">
        <property type="protein sequence ID" value="ENSLOCP00000000904.1"/>
        <property type="gene ID" value="ENSLOCG00000000807.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RQEK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="HOGENOM" id="CLU_047501_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXP7"/>
    <dbReference type="OMA" id="YFLENIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000807">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003081">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="199"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="279" mass="31834" checksum="06B91D9EEDE021B0" modified="2014-04-16" version="1">FSVTGPAHFMIMSLVVPGTHSYQYIYTATVGMSEFPEFVVVGMVDGEQVNYYDSSIKEMIPRREWVKGAVDPDFWNRGTQILLGWQQTFKAGIEILRQRFNQTGGVHTWQLMYGCELDSDGTTRGYFQFGYDGADYISLDKSTLTWTAANQRAVITKNKWDATGADARFQTNYLENTCIEWVKKYVEYGRETLLRTERPQVSVYSRVSGSQGPEVTCLATGFFPKDIVVHLQRDGQDLQEDVHSGEVLPNGDGSFQVRKSLRVSEEELKRHQYSCRVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY18</accession>
    <name>W5LY18_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LY18"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001029.1">
        <property type="protein sequence ID" value="ENSLOCP00000001025.1"/>
        <property type="gene ID" value="ENSLOCG00000000920.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164014"/>
    <dbReference type="HOGENOM" id="CLU_009579_23_0_1"/>
    <dbReference type="InParanoid" id="W5LY18"/>
    <dbReference type="OMA" id="CKVECND"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000920">
        <property type="expression patterns" value="Expressed in bone element"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000669">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="40"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="283" mass="31398" checksum="B67E3DBD952BBD8F" modified="2014-04-16" version="1">MENSTMDNGTAPDELSPRLTGAKIAEIVFCSCIFCIGLPLNCLAVIGLYNLVKANNAQPIYVINLLIADLLQLATLPFTILTFYYFQSIEIIAGLSILIIVYGLSIFVSIGFFVCISLERYVAVVHPLWHQSHRSPRFAIKVSVGVWLFPLLFCIAVTLVLFLLDLRENLPAIGSLLIFVFFFLVPLTLLVFFYVRSKAAITSSIGAPDVKKKRALRVLSAVLVLFIVVYGPFFTLSVINAFLISMGIVTSVGLSLMSVVIRNLSVIVDPILYVFLRNDEHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ71</accession>
    <name>W5LZ71_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-crystallin A chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11 12">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638940.1">
        <property type="nucleotide sequence ID" value="XM_006638877.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ71"/>
    <dbReference type="FunCoup" id="W5LZ71">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001428"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001433.1">
        <property type="protein sequence ID" value="ENSLOCP00000001428.1"/>
        <property type="gene ID" value="ENSLOCG00000001257.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691439"/>
    <dbReference type="KEGG" id="loc:102691439"/>
    <dbReference type="CTD" id="1409"/>
    <dbReference type="eggNOG" id="KOG3591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160159"/>
    <dbReference type="HOGENOM" id="CLU_095001_2_0_1"/>
    <dbReference type="InParanoid" id="W5LZ71"/>
    <dbReference type="OMA" id="QQDDHGY"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001257">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06497">
        <property type="entry name" value="ACD_alphaA-crystallin_HspB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000008">
        <property type="entry name" value="Alpha-crystallin A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003090">
        <property type="entry name" value="Alpha-crystallin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF14">
        <property type="entry name" value="ALPHA-CRYSTALLIN A CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00525">
        <property type="entry name" value="Crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="14">
        <location>
            <begin position="55"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="176" mass="20090" checksum="FAE3B80467BAC9E7" modified="2014-04-16" version="1">MDIAIQHPWFRRALASLNPSRLFDQFFGDGLFEYDLFPYAASTISPYYRQSLFRNFMESANSGMSEVRSDRDKFTIYLDVKHFSPDDLNVKIIDDYVEIQGKHGERQDDHGYISREFHRRYRLPSNMDQSGITCSLSADGILTFCGPKISSGADSSRGDRNIPVTREEKPTSAAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ79</accession>
    <name>W5LZ79_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Actin-like 6A</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022416">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ79"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001436"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001441.1">
        <property type="protein sequence ID" value="ENSLOCP00000001436.1"/>
        <property type="gene ID" value="ENSLOCG00000001256.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156305"/>
    <dbReference type="HOGENOM" id="CLU_027965_6_2_1"/>
    <dbReference type="InParanoid" id="W5LZ79"/>
    <dbReference type="OMA" id="MTEAPWN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001256">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000796">
        <property type="entry name" value="Actin like 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000004">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000009">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000375">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0558">Oxidation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="428" mass="47244" checksum="89BBC4AEA9BACFFA" modified="2014-04-16" version="1">INPSLSVADEVGALVFDIGSYTVRAGYAGEDCPKADFPTVIGVSLDREDGTAPMETEGDKGKQGTTYYIDTNQLRVPREGMEVMSPLKNGMIEDWDSFQAILDHTYKMHVKSEASLHPVLMSEAPWNTRAKREKLTELMFEHYNIPAFFLCKTAVLSSFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFMSMQCRELFQEIGVEIVPPYMIASKEPVREGASANWKKKEKLPQVTRSWHNYMCNTVIQDFQASVLQVSDSPYDEQVAAQMPTVHYELPNGYNCDFGAERLKIPEGLFDPSNAKGLSGNTMLGVGHVVTTSVGMCDIDIRPGLYGSVIVTGGNTLIQGFTDRLNRELSQKTPPSMRLKLIANNTTVERRFSSWIGGSILASLGTFQQMWISKQEYEEGGKQCVDRKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZB1</accession>
    <name>W5LZB1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Matrix metallopeptidase 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZB1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001468"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001473.1">
        <property type="protein sequence ID" value="ENSLOCP00000001468.1"/>
        <property type="gene ID" value="ENSLOCG00000001296.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160903"/>
    <dbReference type="HOGENOM" id="CLU_015489_4_2_1"/>
    <dbReference type="InParanoid" id="W5LZB1"/>
    <dbReference type="OMA" id="GINFLYV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001296">
        <property type="expression patterns" value="Expressed in liver and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF316">
        <property type="entry name" value="STROMELYSIN-2 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="15">
        <location>
            <begin position="39"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="14">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="211" mass="23713" checksum="3F670654C0222635" modified="2014-04-16" version="1">MQRFFGLKVTGRVDRATLSIMKKPRCGVPEMADYSFFPGKPKWTKKRLTYRIVRYTADISKRQVDRTVKTALRLWSKVTPLKFIRINKGEADIMIKFASCRHGDDFPFDGPGGELAHAFAPGKGLLGDAHFDRDETWSLGQSGINLLLVAAHEFGHSLGLGHSQDKDALMYPTYSYRNTAGFQLSRDDIAGIQSLYGTRRTAHRLSKTASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M138</accession>
    <name>W5M138_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase RING2</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING finger protein 1B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">RING finger protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">RING-type E3 ubiquitin transferase RING2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 27">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01034686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M138"/>
    <dbReference type="FunCoup" id="W5M138">
        <property type="interactions" value="1271"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002100.1">
        <property type="protein sequence ID" value="ENSLOCP00000002095.1"/>
        <property type="gene ID" value="ENSLOCG00000001809.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154499"/>
    <dbReference type="HOGENOM" id="CLU_056557_1_1_1"/>
    <dbReference type="InParanoid" id="W5M138"/>
    <dbReference type="OMA" id="PTEMTGD"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001809">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003161">
        <property type="term" value="P:cardiac conduction system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902254">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905304">
        <property type="term" value="P:regulation of cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032330">
        <property type="term" value="P:regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905295">
        <property type="term" value="P:regulation of neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17167">
        <property type="entry name" value="RAWUL_RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16740">
        <property type="entry name" value="RING-HC_RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000067">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000100">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037937">
        <property type="entry name" value="RING2_RAWUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="88"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="201"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="379" mass="42552" checksum="0E746304658CC2A3" modified="2014-04-16" version="1">IQYFVQSKGHISKLPRCFSLWFLIYLNTVVLVSSFERMTQTVQTNGVQPLSKTWELSLYELQRTPQEAITDGLEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARISKHNNQQALSHSIEEGLKIQALNRLQRGKKHQIENGSGAEDNGDSSHCSNASVHSNQEAGPSIKRTKTSDDSGLDMDNANENGAGDMALDGASEIELVFRPHPMLMEKDDAAQTSIGFVPRYIKTSGNATVDHLSKYLAVRLALEELRKNGDANPINVEAASEKQYTIYIPTANSQFTVLNGSFSLELVSEKYWKVNKPMELYFAPTKEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1C3</accession>
    <name>W5M1C3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Phospholipase ABHD3</fullName>
            <ecNumber evidence="3">3.1.1.32</ecNumber>
            <ecNumber evidence="4">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">Abhydrolase domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="32">Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-ditetradecanoyl-sn-glycero-3-phosphocholine + H2O = 1-tetradecanoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54456"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:45240"/>
            <dbReference type="ChEBI" id="CHEBI:64489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:54457"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1,2-ditetradecanoyl-sn-glycero-3-phosphocholine + H2O = 2-tetradecanoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54404"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:45240"/>
            <dbReference type="ChEBI" id="CHEBI:131738"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:54405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40479"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:44811"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-O-hexadecyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = nonanedioate + 1-O-hexadecyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54552"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:78208"/>
            <dbReference type="ChEBI" id="CHEBI:138269"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H2O = 5-oxopentanoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40483"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16120"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77890"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40484"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-hexadecanoyl-2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H2O = 9-oxononanoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61042"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77812"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-hexadecanoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O = glutarate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41159"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30921"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77756"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:41160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = nonanedioate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:78207"/>
            <dbReference type="ChEBI" id="CHEBI:78208"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-octadecanoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:75220"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-octadecanoyl-2-hexanoyl-sn-glycero-3-phosphocholine + H2O = hexanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54464"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17120"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-octadecanoyl-2-nonanoyl-sn-glycero-3-phosphocholine + H2O = nonanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54472"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32361"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54473"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-octadecanoyl-2-octanoyl-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138213"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:54469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-octadecanoyl-2-pentanoyl-sn-glycero-3-phosphocholine + H2O = pentanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31011"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-tetradecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76085"/>
            <dbReference type="ChEBI" id="CHEBI:86162"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-tetradecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76079"/>
            <dbReference type="ChEBI" id="CHEBI:86102"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:54397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-tetradecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 1-tetradecanoyl-sn-glycero-3-phosphocholine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:64489"/>
            <dbReference type="ChEBI" id="CHEBI:86094"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-tetradecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
            <dbReference type="ChEBI" id="CHEBI:86094"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:54389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18689"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="EC" id="3.1.1.32"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18690"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.32" evidence="3"/>
    <dbReference type="EC" id="3.1.1.4" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01004153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1C3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002180"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002185.1">
        <property type="protein sequence ID" value="ENSLOCP00000002180.1"/>
        <property type="gene ID" value="ENSLOCG00000001874.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="HOGENOM" id="CLU_032487_4_0_1"/>
    <dbReference type="InParanoid" id="W5M1C3"/>
    <dbReference type="OMA" id="LDWHGPH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001874">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000079">
        <property type="entry name" value="Abhydrolase domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000952">
        <property type="entry name" value="AB_hydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF50">
        <property type="entry name" value="PHOSPHOLIPASE ABHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01133">
        <property type="entry name" value="UPF0017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 36" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 36" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 36" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="37">
        <location>
            <begin position="153"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="35">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="35">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="35">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00059841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00071303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00082158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="424" mass="47234" checksum="C40CEAA9CD513026" modified="2014-04-16" version="1">ALRVGVRQPGGEAMTAPEFSLNVLTRDISLYLENQVKVGLFGSGVGLSLVLGFSAAYAYYYLSSVAKKPQLVSGGETFCQFLQEHCPVVSETYYPTFWCWEGRIQTLLRPFITAKPWVEYRNELLKMADGGQISLDWFDNDDSERHPDAATRPTVLLLPGLTGTSRESYILHMVQQSRELGYRCVVFNNRGVSNERLLTPRTYCAANTEDLEAAIAHIQSITSAAPLVAAGVSMGGMMLANYLGQKGRETCLRAAAVFSAGWDVFECTASLEKPLNRLLFNSYLTSCLQASVQRHRQILEKLFDIDSIMKANTIREFDERFTSIMFGYPSNDDYYRDASPCHKLKSVQVPTLCLNAADDVFSPGGAIPVEAVRQNPNLAMLVTAHGGHIGFLEGLWPRHSTYMDRVFRQFIKAIFEQGSTLQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1W8</accession>
    <name>W5M1W8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA. Interacts with SWSAP1 and ZSWIM7; involved in homologous recombination repair. Interacts with BLM; required for stimulation of BLM activity by the BCDX2 subcomplex XRCC2:RAD51D.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1W8"/>
    <dbReference type="FunCoup" id="W5M1W8">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002376"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002381.1">
        <property type="protein sequence ID" value="ENSLOCP00000002376.1"/>
        <property type="gene ID" value="ENSLOCG00000002045.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159095"/>
    <dbReference type="HOGENOM" id="CLU_058452_0_0_1"/>
    <dbReference type="InParanoid" id="W5M1W8"/>
    <dbReference type="OMA" id="QRIHTFR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002045">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19489">
        <property type="entry name" value="Rad51D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001042">
        <property type="entry name" value="DNA repair protein RAD51 homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051988">
        <property type="entry name" value="HRR_RAD51_Paralog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047323">
        <property type="entry name" value="Rad51D_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048943">
        <property type="entry name" value="RAD51D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21794">
        <property type="entry name" value="RAD51D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="14">
        <location>
            <begin position="78"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="303"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="336" mass="36523" checksum="4530BF0D3F69E459" modified="2014-04-16" version="1">MVFLREGLCPGLTEEVIQALRTQEIRTVEDLVSSELEELAQKCSISYKALVAIRRVLLTQHTGFPVSGADLYEELLSSMAILSTGNESLDVLLDAGLYTGEVTELAGGPGSGKTQVCLGVAVNISCVLKRTVLYIDTNGGLSASRLLQMVQSKTSNMEEQVEALNRILVCRVFDVFSLLETLHSLRSGGLEQASVRNGPIRAVLVDSVTAVLSHMLGGRQTEGMSLMMQVAGALRLIAKDLNVAVLVTNHVTRDGNGVLKAGLGQSWSHVPRTRVLLQRVDGPQARHSGLRWATLLKSSRQPCHQKQQFDLGSGGQPADSHPDHLQRREQEDVFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3F2</accession>
    <name>W5M3F2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 219</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3F2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002910"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002916.1">
        <property type="protein sequence ID" value="ENSLOCP00000002910.1"/>
        <property type="gene ID" value="ENSLOCG00000002482.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156397"/>
    <dbReference type="HOGENOM" id="CLU_661487_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3F2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002482">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001038">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001140">
        <property type="entry name" value="Zinc finger protein 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="59"/>
            <end position="86"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="295"/>
        </location>
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        <location>
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            <end position="323"/>
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    </feature>
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            <end position="380"/>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="455" mass="47147" checksum="25583C7F6E31EEC1" modified="2014-04-16" version="1">EPSVPPSPTPAVALFLGGADPGSPIPEPPAFRCETCGQRFTQSWFLKGHMRKHKDSLDHKCQVCGRGFKEPWFLKNHMKVHLNKLGLKTGGAGAGAGVGAGPGDPNQAIQNQAFGALYSGLLLARAGGGGGGGGGGGGNKASILGYLGLPGEAAGGTSCVERLQAAAHQVAEMGASAATGGGGERGGGGGGGVAGKMAGSSGGYHAVLTHFQDQGGQVAQLLSWLTEAACQERFQRTENGLRGTPAAPAPAPGPAPALRERVRGTGGKDCPYCGKTFRSSHHLKVHLRVHTGERPYKCPHCDYAGTQSGSLKYHLQRHHREQRNAAAAAATGASVLLPGPPGAVREAGFRGARGGGRRLPQSWATEGPRVKVPKSSRRKPLSTSKFASAAHANGKRGWPGGGFEPLDLSRRPPQDPGEEGVTLHQCLFCPFRTSSAELMAMHLQVNHTSKSRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3Y2</accession>
    <name>W5M3Y2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Period circadian clock 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009657">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3Y2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003090"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003096.1">
        <property type="protein sequence ID" value="ENSLOCP00000003090.1"/>
        <property type="gene ID" value="ENSLOCG00000002607.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3753">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160817"/>
    <dbReference type="HOGENOM" id="CLU_006667_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3Y2"/>
    <dbReference type="OMA" id="GISQCSY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002607">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000013">
        <property type="entry name" value="Period circadian protein homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057310">
        <property type="entry name" value="PER1-3_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048814">
        <property type="entry name" value="Per1-3_PAS-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022728">
        <property type="entry name" value="Period_circadian-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF13">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23170">
        <property type="entry name" value="bHLH_PER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21353">
        <property type="entry name" value="Per3-like_PAS-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12114">
        <property type="entry name" value="Period_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="668"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="780"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="862"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="668"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="785"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="862"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="872"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="905"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="926"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="959"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1006"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1025"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1051"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1165" mass="127191" checksum="7F1BDEAFB636A538" modified="2014-04-16" version="1">RGRDQAHREMMQTVQEMKKRLPSERRGHRKASTVEALNYALNCVKQVQANSEYYKLLMSNGVVLRHDATACNLEELESITSEHTLKNTDTFVAVFSLASGRIVYISEQASSILNCKRKFLDSAKFVELLYHQDINIFYSHTAQPHLPPWNMGTDSAAILFECARVRSFFCRIRSARERSFCFSRHFFHSFEALSLETLQKMRPLLCNVYQSDVMISSHDLPKHEVAMDVKVADCNCTRWIFILQVSDSAVPLLGYLPQDLIGTSVLACLHPEDRPLMLAMHRKILKYAGQSPFDHSPVRFRCQNGDYVTLDTSWSSFINPWSRKVAFVIGRHKVRTGPLNEDVFAPRCKGQLPVIDEEIKELQAQIYKLFLQPVHNNGSSGYGSLGSNGSHEHYISVASSSDSNGNVWEDAQREPVRGVEGCYVILRPKKWGLSLDLSRTQQTMRSCRYGLSAGLPLLSGKQEVRGHLTASQGSSRKQPHVPSYQQINCVDSIIRYLESYSGPALKRKCESLSNTTSSSSEEDRTPPPLLEGTQQVEEESLLATGAPDSQVSAGSGVTTTAAVVGAPLTDLTLSTKAMSVVSVTSQCSYSSTIVHVPQPESEVTALEDAPMGSEQADPPRVALCSAAAPEEFRKVGLTKEVLSAHTQKEEQDYVDRFQHRILQSPYSSYLQQDSSSKAPSHHHGDYVPRPASTTGWRKSKTGKPKHKRPKPQGSSDSYASPPESCRARRPPAAPASPLYQAWTPPEASQARAPSMGFLPPVVMPFQPAYFPLPAQVGGEAAGGMTPNSSSQQGGNPLAMQGVQPMQPAQPVHSVQAMQTMQPPLPVQQMQGMVPYVAPVMAVFVPSFPNYPGYPMYPQEVGSVGPASSPSPQAVPCPSFTPAPFPETHPPQGPYIGPPEPPLPSLSPQAPSSLGEDPDSAQPPPLFSSSRSSSPLQLNLLQEELPKPAEPQGGAALPESLHEPHTKGDDAPSESGNQDNQSTSSELLDLLLHEDSRSGTGSNVSGSGSGESGGSLGSGSNGSGSNGTSASHTGSSQSGNSSKYFASNDSSDTSRSKARKSQEVPERASFEKRAEDSLWTMIQHTPEPIMMNYQLLPRDQAQVLTEDRKKLLLLQPLQPWFTEEQKEELAEVHPWICQHGLPPEIDTQGCISCNRTADPPAEPQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5M411</accession>
    <name>W5M411_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Decapping enzyme, scavenger</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M411"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003119"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003126.1">
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        <property type="gene ID" value="ENSLOCG00000002653.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003924"/>
    <dbReference type="HOGENOM" id="CLU_1096402_0_0_1"/>
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        <property type="component" value="Linkage group LG26"/>
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    <dbReference type="Bgee" id="ENSLOCG00000002653">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DcpS/DCS2"/>
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        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="254" mass="27651" checksum="3B4EF1F61AE63303" modified="2014-04-16" version="1">GTTSTAPITCGRPPISVWVYNILEKKAEADRIVYEDPDPDVGFVLLPDFKWDQKQLDDLYLIAIVHRRDIRSLRDLTAAHLPLLLNIQQRGQVREPRAAHPDVHWSETLSRETHSKRSHQRVAGSPLGSISGKGGQVRGSGHGSSVLFLFLNLGPATSALHEHSGPENCLNWPNAFHLDVLKASSRPGCGASYGNQECPLALGGDVKGQCVPYAAEILAESRRNSEMALCGVSRIGCFPQNGSGLKAEPSHLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M491</accession>
    <name>W5M491_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centromere protein C</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-C/MIF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M491">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003199"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003206.1">
        <property type="protein sequence ID" value="ENSLOCP00000003199.1"/>
        <property type="gene ID" value="ENSLOCG00000002724.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079480"/>
    <dbReference type="KEGG" id="loc:107079480"/>
    <dbReference type="eggNOG" id="ENOG502S2KA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282920"/>
    <dbReference type="HOGENOM" id="CLU_259192_0_0_1"/>
    <dbReference type="InParanoid" id="W5M491"/>
    <dbReference type="OMA" id="HSATTIF"/>
    <dbReference type="OrthoDB" id="1939643at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002724">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028386">
        <property type="entry name" value="CENP-C/Mif2/cnp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025974">
        <property type="entry name" value="Mif2/CENP-C_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684:SF11">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11699">
        <property type="entry name" value="CENP-C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mif2/CENP-C cupin" evidence="6">
        <location>
            <begin position="1240"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="247"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="625"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="728"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="770"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="834"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="910"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="729"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="912"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1329" mass="148995" checksum="1FD3FE34A7B3E185" modified="2014-04-16" version="1">MDNKLQSLGRASPKLRYFPQKNERFETIQKGGGDMHVLDDVFEDSDSSPLSFCDMNMPSPVPKSSSTPRCLDKKLILLSQQKSESQYQIPVSHEDSAVVQKTSQNRASPLKGQGSLQMESSSQVACETDLSRESDISAVIEIEVGTLEKIPSTTEAEGMSMCCRKLPEEENAQKSFSSNNDEFESLAKKKAENPLCVPSHETHSSDEEPKKMTSSDDECALLNPSPSPKELKITDALPHEKWKKSLLDVEKQPKNSPIATTSIPKKANETSSFLQKLKNRGRSELLRTKKKESPMKDPPLPELDEDFMILEEENFKWENWISIPKKKCTSSNQPQHDINENVKKKTAKEAEPSKTSAKSPKNEHNIVDITAGENKRKEDRGKQSQKKKTKENSKKNQTNNFVKENTHVDADVVQNKKKEAQRTQSRKKRIKDSSSKKQENDYFDEDAQVQDKIKTAPKAKEAKKRKLSTEAPVPVKAKIKFTDARRNTMGTETQTNKSLNKDGDLNDHCPSYSSETEDAVIKPVKQKSKDSKGMCRSAKVTQEELLKKNIKTEKKALIGVLTSPEQIHLKPAHEKRQCKPPQPWWLMGQEESINVNLNRNGVKEATLKKHKSKQTEVLQASEAQTVMKKQHTRNTRHKVSNSTEAKAKKTTRQRKKTNTEPISDILKPSDHELKMHVRVQHEGDGQEISDAYINEEDHSISPLHSSCPNLSKAPLGKKIISDAQQLKVNTRKGKKAKPKQPRKTQSKLSKTRSNYKAAASLAMEICEEQTNSVNEEEALSSTSPPLSSRTKELASTPERTFPGHAIISEDPSEEHSLPERHILKTLSTIQKQLERPKGGYFSSPVHQKRQRKPPGNWWEPKDPGQPGSSAAKPSRKQASILKRSSVNKCMERRANNVNLKKVCSGEERKDCNTLSDTVSGPTAANLKPKHSKIKQPIIADYLNGPRNLRASLASFDAICTPLNVPQRLTPRTKESTVPRAASEKKNKILCKQKSKKTYFSPSQTDQISSAAPKSFQVHQHSKGHTQCQPVPNTQEMLGGFLPLADYHQDGVSPLRDFPASELKLNCVSIPEFCPVSEEHVSVEAQGANEEMLSSIPSDQAHHFREAQPSVSYIGKQQKRRSSLQHLRIGRHSGCASYQGYESGPSSMRTIHYEDDVLVDCEQENSFLAGKKSCLQVSENPASGMSLNQMELTTAEKERIYLWLKKSWTTTITCFAESVVLDDFDWYESSGKFVGLVKNVKCPLFSQGKILLGPFMKKNSQLVVSDAMIFNVLAGTVHVKIDLQEQNLSSGDVFCISPGQMYSVLNMCEEPALLLYTQMKYSKTNNMEDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4B8</accession>
    <name>W5M4B8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4B8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003226"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003233.1">
        <property type="protein sequence ID" value="ENSLOCP00000003226.1"/>
        <property type="gene ID" value="ENSLOCG00000002741.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="HOGENOM" id="CLU_2090278_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4B8"/>
    <dbReference type="OMA" id="GNEQSCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002741">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867595" description="Receptor ligand binding region domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13213" checksum="8BC5AD8699CC500B" modified="2014-04-16" version="1">MLLQILLCMMFLALFTSEGQPLCKRQGKPAAPHLLRENNIMIGGIFALHRDAQEKIFQFTTEPQPLKCKSFSFAEFQSVQTMIFAIEEVNNRTDLLPGISLGYKIYDSCDSLPSAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4L5</accession>
    <name>W5M4L5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the oligosaccharyltransferase (OST) complex. There are 2 OST complexes, OST-A and OST-B, which contain STT3A or STT3B as catalytic subunit, respectively. OST-A and OST-B contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, and OST-A contains DC2/OSTC and KRTCAP2/KCP2 specific accessory subunits. OST-A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the OST-A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The OST-A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01024149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4L5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003323"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003330.1">
        <property type="protein sequence ID" value="ENSLOCP00000003323.1"/>
        <property type="gene ID" value="ENSLOCG00000002777.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156655"/>
    <dbReference type="InParanoid" id="W5M4L5"/>
    <dbReference type="OMA" id="TWYAIGT"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002777">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054479">
        <property type="entry name" value="AglB-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF43">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22627">
        <property type="entry name" value="AglB_core-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="21">
        <location>
            <begin position="17"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="AglB-like core" evidence="22">
        <location>
            <begin position="533"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="726" mass="82983" checksum="D00744339740A107" modified="2014-04-16" version="1">MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFSVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDVFCAYVRSVLFTASCSPGWLMITSAALYHVLHFFHITIDIRNVCVFLAPLFSSFTAIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWSSMCSLAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVQSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEILFKSVISLVGFILLSVGTVLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFNNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLTTYMKNLDVSRPDKKSKKQQDSTYPNRSTKDTKIANCFHVGHSPPLSRQVASGMILIMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGRHIKEHDYYTPTGEFRVDRDGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGYDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4X3</accession>
    <name>W5M4X3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase TRIM39-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4X3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003431"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003438.1">
        <property type="protein sequence ID" value="ENSLOCP00000003431.1"/>
        <property type="gene ID" value="ENSLOCG00000002915.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102683306"/>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240385"/>
    <dbReference type="InParanoid" id="W5M4X3"/>
    <dbReference type="OMA" id="CGDVRMN"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002915">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="396"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="253"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="596" mass="67197" checksum="BF1B61A924049562" modified="2014-04-16" version="1">MISPGSLLSEEQLLCSICLDVFASPVSLPCGHNFCQACIGGYWDSGGACQCPLCKEAFGRRPSLQINRTLNEIAEQFKATRSSRADGGAAGPGEVPCDVCAAEKLRAVKSCLVCLASYCDEHVQPHREVGRLSRHRLVDPARSLRDKLCRKHGRQLELFCRRDQAFICRLCTKKDHRTHSTVPIEAQRLELQNNMRKTQAHLQQMAQDRLKKVEDVKKSVKLHKRWVQKEMKDSVKLCTALAHCVERSQAEVIKALEDKQRAAEVQAEGLIKELEQEMAAIKRRNAELEQLARTEDHLLFLQCFPSLCSPPQTKEWSSITLPTEPSADSSWEALTHLEERFLEELRKLKNKGPQDNPARRRSSCSWRTMVLSYMWTVILTAVLLGFYAHTHMQKQTPQDEMRNAQISSTSVRMVNVTLDSSTAHPSLILSEDGKQVRLGDAPQDLPDNPERFDQDFCVLGAEGFTSGRHYWEVEVGDKTEWDLGVARESVDRKGEITLSPEDGYWSIWLKNGKEYQALTDNAVALPLSTRPRKVGVYVDYEGGQVSFYNVEASSHIYTFMASFTEKLYPYFSPGLNSDGKNSAPLIISSPAHIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M550</accession>
    <name>W5M550_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Osteoclast-stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6F0</accession>
    <name>W5M6F0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein lin-28 homolog A</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lin-28 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG A"/>
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        <property type="entry name" value="COLDSHOCK"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="32"/>
            <end position="104"/>
        </location>
    </feature>
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            <end position="181"/>
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    </feature>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="203" mass="22175" checksum="CD48E928C2236CE3" modified="2014-04-16" version="1">MGSVSNQQFTGGCAKTEDEESPSTEEDSRSFQGSGVCKWFNVRMGFGFLSMTNREGIQLDTPVDVFVHQSKLHMEGFRSLKEGEVVEFTFKKSAKGLESLRVTGPGGVPCVGSERRPKGKSIQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPIKAQQSSPSSQGRPASLKGEEEEQGHAPLPPESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7P1</accession>
    <name>W5M7P1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="8">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01004333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7P1"/>
    <dbReference type="FunCoup" id="W5M7P1">
        <property type="interactions" value="1794"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004399"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004407.1">
        <property type="protein sequence ID" value="ENSLOCP00000004399.1"/>
        <property type="gene ID" value="ENSLOCG00000003697.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0427">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156908"/>
    <dbReference type="HOGENOM" id="CLU_030988_15_1_1"/>
    <dbReference type="InParanoid" id="W5M7P1"/>
    <dbReference type="OMA" id="WQMDIKV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003697">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="18187" checksum="FF9D54E34EA04FBF" modified="2014-04-16" version="1">TSVIGENLLHYYKRLQKELLALQNDPPPGMTLNEKSVQNTITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAM9</accession>
    <name>W5MAM9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAM9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005438"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005446.1">
        <property type="protein sequence ID" value="ENSLOCP00000005438.1"/>
        <property type="gene ID" value="ENSLOCG00000004548.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXPD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263479"/>
    <dbReference type="HOGENOM" id="CLU_024555_7_1_1"/>
    <dbReference type="InParanoid" id="W5MAM9"/>
    <dbReference type="OMA" id="HWISSAK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004548">
        <property type="expression patterns" value="Expressed in liver and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF20">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="70"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="164"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="247"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="340"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="420" mass="44530" checksum="11D167E67CA6658A" modified="2014-04-16" version="1">GRDAELVCSADCHPPCSYTWFFNGVQVANGSVVTMRPVTYTDNGVLACEASSSGTGLSVRVFRVITVADPNATVSIQPAHALPIADQPFSLTCNVSGAQVTSRQWVKDDLLLTASSTVTFSADNSTVFFSPLLLLDNAVYQCLADPGTHWISSAKYQLRVIYGPQWLTVAGPHLLAAGGNYTFACSADCFPPCLYAWELGGSPIGQGRTLTLRQVTHRDRGLLTCRASNTASGLTARASEAIAVMEPITVVAVRPAGQLPIQDQAFNLSCEVTGPVVSRSWLKDGQPLPPSDRTSFSQDNSTLSFSAALLSDDGLYQCAASNPVSNRTSPGYQLLVNYGPETPVIAGPQEVQTGRTVTLSCSAASQPPSLYYWSVNGTRAGEGSVLQIGQVSPSNTGLYTCTARNTVTNRTSSAERRLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAU3</accession>
    <name>W5MAU3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein ABHD15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha/beta hydrolase domain-containing protein 15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May regulate adipocyte lipolysis and liver lipid accumulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PDE3B; this interaction regulates PDE3B's stability and expression and, thereby, impacts the antilipolytic action of insulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAU3"/>
    <dbReference type="FunCoup" id="W5MAU3">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005502"/>
    <dbReference type="ESTHER" id="lepoc-w5mau3">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000005510.1">
        <property type="protein sequence ID" value="ENSLOCP00000005502.1"/>
        <property type="gene ID" value="ENSLOCG00000004594.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="HOGENOM" id="CLU_032487_3_0_1"/>
    <dbReference type="InParanoid" id="W5MAU3"/>
    <dbReference type="OMA" id="FENGLCW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004594">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000103">
        <property type="entry name" value="Abhydrolase domain-containing 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF78">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING 15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="476" mass="52983" checksum="5EB6A519C1FDE357" modified="2014-04-16" version="1">EMLEWFAAVFVLIAVALLWPAKRYFGFEPWPGVLLGGLGRKTARRPSRELDRCVSDPCPAAGAPAGEEPVSLICKPSALANYLLKHCVTFSNFATALTWSWRASPFLQTVFSACWPLESSVHFMREYLQLSDDGLVALDWAVAGSAHHRRRRTSSNSTSPILLVIPNSFGKITRNVLKLCQVGLAHGYLPVVFSRRSQNGTPLSTPRLQQFGDPADLREAVRYIRYRQPAGRLYAVSESTGAGLLLSYLGECGSSSYVTAAACLSPIFRCQSWFESGPPWPFRWALLLYQKACLSRYKTALGDMVQMDKLFSSCSLRALEEALFCHPALTGGWEAYWERNDPLRDVDEVAIPVLCLCSRDDPVRGAPAATLPFELFETNPHFFLLLTAQGGHCGFSTPGEEGALQWSHQALLEFFRATTDFFAAEERSKQAARRRGLGGGTNVRAFRHRSVSTCKRVPACSHNIHAIFSWQRSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB32</accession>
    <name>W5MB32_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ring finger protein 216</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637308.1">
        <property type="nucleotide sequence ID" value="XM_006637245.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216020.1">
        <property type="nucleotide sequence ID" value="XM_015360534.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069036836.1">
        <property type="nucleotide sequence ID" value="XM_069180735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB32"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005591"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005599.1">
        <property type="protein sequence ID" value="ENSLOCP00000005591.1"/>
        <property type="gene ID" value="ENSLOCG00000004666.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686282"/>
    <dbReference type="KEGG" id="loc:102686282"/>
    <dbReference type="CTD" id="54476"/>
    <dbReference type="eggNOG" id="KOG1812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048032"/>
    <dbReference type="InParanoid" id="W5MB32"/>
    <dbReference type="OMA" id="FRCENEA"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004666">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20339">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16630">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000016">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF216 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047545">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058758">
        <property type="entry name" value="UBA_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF47">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26112">
        <property type="entry name" value="UBA_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CUE" evidence="9">
        <location>
            <begin position="314"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="542"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="785"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="895" mass="101587" checksum="965D7F18D7912CFA" modified="2014-04-16" version="1">MADGEGDEEVIHLASFHRHRGEDDRIRNDLLITISDDSDDELPTLMPNNPAQIKAELEDEDDEEEDVVILEPLRPQQPLRPNVIRPAALWGGTPHQETPERQGEAPMEAPHPAAERRILLARRRLPLGNVDLESGNNALNVLSGNQKATPSSSHAPPVPRPHPPAGTETTLEQGPAGRAAVCNGEAFIAAMPEPLAHRDVRHGIPLGSDRSELRPLNDQPHTDQERQAGVVPKRTEPLHVPLPALPEQQQPHESLRFPPALGVQENQHQAPPGPVLHIPLAVNQNEPGALIDDERPGPSIPNPELIAARYDRDQIKSLVHRVAEFFPDIEEAYVEELIKSHDLNDLNLICNFLLENPNYPKKEGTSVLNQSSILLETNEDENQNKENYFDFSKLEAVDQRTAVQAADLLMADFKMLSSMDIKWALHSLRGHYAITRKALSDAIKKWRERSPESSGKKKKRKEMNQFSYIDFKFEEGTTKFERRMFFLENKRRHWKSYDKRSLHPSLQKEITFYEQKAKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFAFEDLTQCADGHLFCKECLVKYAQEAVFGSGRSELSCMDGNCTCAFPTSELEKVLPENILCKYYERQAEEAVAATCADELVRCPFCNFPALLDKEVFLFSCPNPRCRKETCRKCQILWKDHMDLTCEQVAEKDEIRLRVAFEEKMTAARVRKCHKCGTGLVKSEGCNRMSCRCGAYMCYLCREPINGYNHFCQHARSPGAQCRHCKKCSLWTDPTQDDERIIQEIQREAEEELRKKNSENTLKRVGPPPEALPPKLPRAADPGARIPLDPPPAPQHLLQGPPLLPPHMMLPRLPPQPMNMPIPAPYVPPLPNLPVNYVLPNLNLDLNLPMHYGPHHPFFRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBQ9</accession>
    <name>W5MBQ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphoinositide-3-kinase interacting protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBQ9"/>
    <dbReference type="FunCoup" id="W5MBQ9">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005818"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005826.1">
        <property type="protein sequence ID" value="ENSLOCP00000005818.1"/>
        <property type="gene ID" value="ENSLOCG00000004841.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTWD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017774"/>
    <dbReference type="HOGENOM" id="CLU_092099_0_0_1"/>
    <dbReference type="InParanoid" id="W5MBQ9"/>
    <dbReference type="OMA" id="HDQKVCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004841">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050759">
        <property type="entry name" value="Serine_protease_kringle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261:SF16">
        <property type="entry name" value="PHOSPHOINOSITIDE-3-KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261">
        <property type="entry name" value="PLASMINOGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0420">Kringle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865919" evidence="6">
        <location>
            <begin position="24"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="7">
        <location>
            <begin position="29"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="28831" checksum="59F27B70AF0A4295" modified="2014-04-16" version="1">LLLARKMLFIAPFLLLNIAFVDGASVIQECIRSNGTEYRGEKQTTASGYQCLNWTLVTRDYNITAHPDTETGVGNHSYCRNPDGSALPWCYVTSNSSEVKRQLCAFEICKDQATPAAVTEGFRASSSSEQRIFESAESLSSQSEGAAVQPVIGISQRSGPKQKKDLGTLGYVMGILMMAVIIILGVGITLGYLYKRGRDLKKQHDQRVYEREMQRITLPLSAFSNPTCEIVDENTIVISTHQTPVEDVVEGSDPLMGQAGTPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBX3</accession>
    <name>W5MBX3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PYD and CARD domain containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Inflammasome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBX3"/>
    <dbReference type="FunCoup" id="W5MBX3">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005882"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005890.1">
        <property type="protein sequence ID" value="ENSLOCP00000005882.1"/>
        <property type="gene ID" value="ENSLOCG00000004898.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3G5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164898"/>
    <dbReference type="HOGENOM" id="CLU_113553_0_0_1"/>
    <dbReference type="InParanoid" id="W5MBX3"/>
    <dbReference type="OMA" id="EVMINVY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004898">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072559">
        <property type="term" value="C:NLRP3 inflammasome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140608">
        <property type="term" value="F:cysteine-type endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08330">
        <property type="entry name" value="CARD_ASC_NALP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08321">
        <property type="entry name" value="Pyrin_ASC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033516">
        <property type="entry name" value="CARD8/ASC/NALP1_CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051249">
        <property type="entry name" value="NLRP_Inflammasome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985:SF2">
        <property type="entry name" value="APOPTOSIS-ASSOCIATED SPECK-LIKE PROTEIN CONTAINING A CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1271">Inflammasome</keyword>
    <keyword evidence="5" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="7">
        <location>
            <begin position="112"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="23037" checksum="4B1200C84B0A715D" modified="2014-04-16" version="1">MAKSVKDHILETLEDLEEKDLKKFKNKLCDREGEPKVPRGRVQKADAIDLVNLLVSYFTERGAAEVAIEVLRAINLNQEADELKNGTGKPTAFQAPSEKKMEEECRKTRFFFASTEVHFVDRHRSALIQRVTMVAPILDALLTKKYVSQEEYSLILEERTPASRMRALLTGPMVTCGKRGKDELCRILEQEQPILMEDLMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBY3</accession>
    <name>W5MBY3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01018350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBY3"/>
    <dbReference type="FunCoup" id="W5MBY3">
        <property type="interactions" value="1101"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005892"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005900.1">
        <property type="protein sequence ID" value="ENSLOCP00000005892.1"/>
        <property type="gene ID" value="ENSLOCG00000004901.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075011"/>
    <dbReference type="InParanoid" id="W5MBY3"/>
    <dbReference type="OMA" id="CLRFRHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004901">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000287">
        <property type="entry name" value="Cell division cycle 7-related protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="46"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="545" mass="59841" checksum="BDF9299210D72D07" modified="2014-04-16" version="1">CKKMHVVNKQSHESADASSFSHQVCREIKTDIESLYEAIPQLSKIFVILDKIGEGLTGTFSSVYLAEARLTSGAKEKFALKHLIPTSHPVRIAAELQCLTVAGGKENVIGVTYCFRKDDHVVIVMPYLQHESFVDIVSSMGFGEVRRYMFHLLKALRHIHSFGIIHRDIKPSNFLYSRQQQKYALVDFGLAQGTPGTQIELLRVLKAESQQGASTDNRPPAPPESKTQPCRPSPDDRPLHCPMKPPAKRSWSLAQTKHRKEAQLVNSAQRSVFGERNLNKLNTREDSLVKVMEGFLISSPVKHSKVLDVASRRMAVPARRPVAVKGAPGAQSRRPAGGCPARLTCSCHDTDRVCNMCLSRKPQVAPRAGTPGFRAPEVLTKCPHQGTAIDMWSAGVVLLSLLSGRYPFFKASDDLIALAQIMTIRGSKETIQAAKTFGKSLVCSRELPAQDLRTLCERLRGAQRRSESGGPDPSAAPGALEEPSLAARLGPPPEPPQGGQRDSGEEGWDRVPDAAYDLLDRLLDLNPATRITASQALLHPLFQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC91</accession>
    <name>W5MC91_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cadherin 16, KSP-cadherin</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01010509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC91"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006000"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006008.1">
        <property type="protein sequence ID" value="ENSLOCP00000006000.1"/>
        <property type="gene ID" value="ENSLOCG00000004983.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165234"/>
    <dbReference type="HOGENOM" id="CLU_016170_0_0_1"/>
    <dbReference type="InParanoid" id="W5MC91"/>
    <dbReference type="OMA" id="INLPTVC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004983">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000188">
        <property type="entry name" value="Cadherin 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000123">
        <property type="entry name" value="Protocadherin beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF424">
        <property type="entry name" value="CADHERIN-16 ISOFORM X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="55"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="376"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="478"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="586"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="745" mass="82259" checksum="B33DB59AE680351E" modified="2014-04-16" version="1">GVESGFLYALKPFDRERLPSYSVAVTNSTVPNGILQCITIEVFVIDMNDNVSEFSEESMTGIVQTGLIAGVPFMQVKATDLDDPTTANAELHYSLIDQSRSAPSSSMFSIDAQTGSVSLTAEGASSLDPEVCHQYNMTANVDINGKPTAYFSTDIVMIDVITNTWTSLEPVHLQENLPAPFKVFASQAQWSGSQVEDTLKRDFPELLFAISRDGIYVKSPLDRKAQAEFQIEVFVENSNGKVIAKPLELLVNKPTFSQPECHVVTKKLTTKVTCSVTQQPCVSKTEKTLTVKVLGRLLPDGYANNLQVCSDSNAISIQASIIRGVPQGSVLGAMLFSWVNCHCYANEDSTVYLCLKSDIKNSGPGYDLNDYGPFLIPEDTKVGVQIMTVVASDRDKPGTDIWFVESANKGENFKLVTVRETNKTTILIFKRERLKFNKNNEEYTRVLRGRNTAALSGAESTATVSVRVGNVNEAPILSQSSYVVTVKEEEQPSCILFTVEGSDPDSTYKYPVNYFLKGDSKKYFTINKDSGEIRGLPCDREVDSTYTVEAVEEDSGKTSLILVTNVTVFMHDVNDNVPVLVGDYSESTPRNETQALVISATDSDGTQHGGALQFSLRKDTIVHQYWRLDPVYDTHASLPMQNSYLAPGIYMVPIIISDSEIILLITPIQNCRITVTVCNVRWNCRMAVKELSRMPTVQSAVGTLLGTLGVLGKYLLLIIFFQLSQSIFKMAFKIHLATLFVWVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCP6</accession>
    <name>W5MCP6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">B-cell receptor CD22-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015224199.1">
        <property type="nucleotide sequence ID" value="XM_015368713.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCP6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006155"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006163.1">
        <property type="protein sequence ID" value="ENSLOCP00000006155.1"/>
        <property type="gene ID" value="ENSLOCG00000005113.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686857"/>
    <dbReference type="KEGG" id="loc:102686857"/>
    <dbReference type="eggNOG" id="KOG4475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222294"/>
    <dbReference type="InParanoid" id="W5MCP6"/>
    <dbReference type="OMA" id="TIKYHED"/>
    <dbReference type="OrthoDB" id="9448246at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005113">
        <property type="expression patterns" value="Expressed in bone element and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867948" evidence="3">
        <location>
            <begin position="23"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="137"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="238"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="436" mass="48343" checksum="CBC01DEE6979F8F7" modified="2014-04-16" version="1">MGVEYSLSLWGMTLLILSGVYGSDWAVSYPRRTICTVRGSSVIIRCSYNYPKEFTVHSQSVQHKVTTVMWCKTNTTCNENISIYNSDNEPFRQKIVYLGNKQKNCTLMINDVEEIDNGRYKFRFQTNNPGGQWTGNPGVTLTVSATPWKVKANSTRKKDTFKEGDSVTLMCHTGSCFPTTTELTWFKNGQPISNRQTTANTLHFNSVSYEDYGNYSCASKANTSISSPQFSLDVKYSPKSTSVSVSPSGQILEGSSVTLTCRSNANPPVKNYIWFKMSSAGVQETGQGQNLTLAEVSSGDSGHYFCGAQNKHGAENSTAVVLAVAAKQNPAVLPVVLVVILLSISALVSYICLKRKRVDPSEEESNRNTDTEETKDHTYGNMVPSHNPKRTQEQSVNEEDDVNYSTVHFLSKTTPRNELPFEETEDASVLYSIVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCP8</accession>
    <name>W5MCP8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Lysine-specific demethylase 2B</fullName>
            <ecNumber evidence="5">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">F-box and leucine-rich repeat protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="34">F-box protein FBL10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">F-box/LRR-repeat protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">JmjC domain-containing histone demethylation protein 1B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">[Histone-H3]-lysine-36 demethylase 1B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01005121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006157"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006165.1">
        <property type="protein sequence ID" value="ENSLOCP00000006157.1"/>
        <property type="gene ID" value="ENSLOCG00000005104.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1633">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154717"/>
    <dbReference type="HOGENOM" id="CLU_003540_0_1_1"/>
    <dbReference type="InParanoid" id="W5MCP8"/>
    <dbReference type="OMA" id="HTHLTHY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005104">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032452">
        <property type="term" value="F:histone demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21785">
        <property type="entry name" value="CTD_KDM2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22180">
        <property type="entry name" value="F-box_FBXL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15644">
        <property type="entry name" value="PHD_KDM2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1360:FF:000002">
        <property type="entry name" value="Lysine (K)-specific demethylase 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000005">
        <property type="entry name" value="lysine-specific demethylase 2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000020">
        <property type="entry name" value="lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000011">
        <property type="entry name" value="Lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="20" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="25" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="22" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="42" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="26" id="KW-0804">Transcription</keyword>
    <keyword evidence="23" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 36" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjC" evidence="40">
        <location>
            <begin position="150"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="39">
        <location>
            <begin position="557"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="38">
        <location>
            <begin position="610"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="374"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="701"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="933"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="37">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="701"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="720"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="37">
        <location>
            <begin position="797"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="37">
        <location>
            <begin position="832"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="37">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="37">
        <location>
            <begin position="877"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="909"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="37">
        <location>
            <begin position="961"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="37">
        <location>
            <begin position="979"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006942"/>
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            <dbReference type="ARBA" id="ARBA00013246"/>
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            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00056806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069294"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00080420"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1297" mass="146773" checksum="273C1B79C7313C2E" modified="2014-04-16" version="1">MEAHAESGRRLTQRSIGRRMYDENEDLSDVEEITSIRSFNLEEKLKSDSYNTNYVVMMDGKDFTYEYVQREALKIPLIFKTKDGLGIRRMPDPDFNVSEVKGLVGSRRAVDVMDVSTQKGTEMSMAQFVRYYETPEAERDKLFNVISLEFSHTKLENLIKRPIVVDLVDWVDNMWPQHLKERQTEATNIIADMKYPKVQKYCLMSVKGCYTDFHIDFGGTSVWYHVHRGGKVFWLIPPTPPNLSVYEEWVLSGKQSDVFLGDRVDGCQRIELKQGYTFFIPSGWIHAVYTPEDTLVFGGNILHSFNIPMQLTIYEIENRTKVHAKFRYPFYYEMCWYVLERYVYCLTKRSHLIKGFQRESMLIDEERKLSTESFFSDSQMDMEEDSCEAQSREERAGTQPASEGKDKKPRAAAAARHLKHTLSNDSDDSSKSATAAAPPDFPKTPSGSPVSEASNKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEDMKVVLKEHADDDPKLAITGVPVVCWPKKSVKQRAGSRPKGKMGASTAVKLAANRATAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTLEEEKEKFNVMLMECSICNEIVHPGCLKVKDAGGLVNDELPNCWECPKCNHAGKTGKQKRGPGFKYASNLPGSLLKEQRVNREVKEEAEPAAAAKRKAEREENPGKRKPEEPAKKRAPALLPDALPRPAPEETPLRKKRKLFDKGAEPSTRKRRKSWKTPDDAKPLLQIKTEQDEELEEEEEEDPEGEEKEAEEREAEQEREAEEEAAVAVAAAAAGPEGEREETEEEAGCEDGGEDLLAVPQKTPARESDQSRSSSPQAGPSSEGGSEPQEKGRLRARRKRRLPNKELSKELSKELNLEIQKTEDCLANENCQPLKSEPESENEEPKRTLNNGGELQPAQQQQLSPLGFNRSPPRTPVSGRPPPSRSPPKGMQMERHVIRPPPISPPPDCLPLDDGESHVMRREVWMAVFGYLTHKELCVCMRVCKTWNRWCCDKRLWTKIDLNRCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRVLLLSGCSWVAVSALCTSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQIDNRSKLRNVVDLRLAGLDITDASLRLIIRHMPLLSRLDLSYCNHITDQSINILTAVGTTTRDSLTEINLSVCNRVTDQCLTYFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGLVEEKLLQKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDT5</accession>
    <name>W5MDT5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutamate pyruvate transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamic--alanine transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutamic--pyruvic transaminase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01034964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDT5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006544"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006552.1">
        <property type="protein sequence ID" value="ENSLOCP00000006544.1"/>
        <property type="gene ID" value="ENSLOCG00000005426.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682267"/>
    <dbReference type="KEGG" id="loc:102682267"/>
    <dbReference type="CTD" id="84706"/>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159061"/>
    <dbReference type="HOGENOM" id="CLU_014254_3_1_1"/>
    <dbReference type="InParanoid" id="W5MDT5"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005426">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000226">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000345">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF311">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="14">
        <location>
            <begin position="153"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="555" mass="61821" checksum="BF686C0CBECAB5FC" modified="2014-04-16" version="1">MHRFQVMAKRSLIAGVFDHCSCIVRMNAVKTPCSIQPLKHKRHVTELQGVRRLSTAEASVAARENGKIREKTLTMETMNPQIKTVEYAVRGPIVIKAGEIERELQEGVKKPFTEVIKANIGDAHAMGQKPITFLRQVVALCTCPELLDSPAFPEDAKKRARRILQSCGGNSLGSYSASQGLDCIRENIALYMEQRDRGVPANPDNIYLTTGASDGIVSILKLLVAGEGLSRTGVMIPIPQYPLYSAAISELGAVQVNYYLDEEHCWALDVNELHKALSAARKYCNPKVLCIINPGNPTGQVQSKKCIEDVIHFAYEEKLFILSDEVYQDNIYSPECQFHSFKKVLYEMGPEYFNTVELASFHSTSKGYMGECGFRGGYMEVINLDPEVKAQMVKLLSVRLCPPVSGQAAMDVIVNPPRPGEPSFEQFIQEKNAVLNTLAEKAKLTEKILNTLPGIKCNPLQGAMYAFPRIFIPPRAVEEAKALGMPPDMLYCLKLLEETGICIVPGSGFGQKEGTYHFRMTILPPTEKLKLLLEKVRDFHIKFLQEYSTLDSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME86</accession>
    <name>W5ME86_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucolectin tachylectin-4 pentraxin-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME86"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006695"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006703.1">
        <property type="protein sequence ID" value="ENSLOCP00000006695.1"/>
        <property type="gene ID" value="ENSLOCG00000005549.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQVA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248575"/>
    <dbReference type="HOGENOM" id="CLU_072164_2_0_1"/>
    <dbReference type="InParanoid" id="W5ME86"/>
    <dbReference type="OMA" id="SFICIML"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005549">
        <property type="expression patterns" value="Expressed in bone element and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF8">
        <property type="entry name" value="SI:CH211-215K15.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866036" description="Fucolectin tachylectin-4 pentraxin-1 domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="11">
        <location>
            <begin position="26"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="172" mass="19129" checksum="3D2FFDCEFA5EF991" modified="2014-04-16" version="1">TVQHNSISPIILLLIQATMLSISPAEQNVALYGRPTQSSVLELGQAINAVDGNTESDFNRGSCSSTRLQNSPWWRVDLLAEHNISPLRITNRDDDCCRGWLAGAQVLVGNHQDHNWNQWCGEISSGGQVAREFCCLGMMGRYVSVTHHNVNRILSLCEVEVFRVPVRDENCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF46</accession>
    <name>W5MF46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024385">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639687.1">
        <property type="nucleotide sequence ID" value="XM_006639624.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069035435.1">
        <property type="nucleotide sequence ID" value="XM_069179334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF46"/>
    <dbReference type="FunCoup" id="W5MF46">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007005"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007013.1">
        <property type="protein sequence ID" value="ENSLOCP00000007005.1"/>
        <property type="gene ID" value="ENSLOCG00000005800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688272"/>
    <dbReference type="KEGG" id="loc:102688272"/>
    <dbReference type="CTD" id="9371"/>
    <dbReference type="eggNOG" id="KOG4280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="HOGENOM" id="CLU_001485_22_4_1"/>
    <dbReference type="InParanoid" id="W5MF46"/>
    <dbReference type="OMA" id="LESKMLC"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005800">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048884">
        <property type="term" value="P:neuromast development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043584">
        <property type="term" value="P:nose development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="9"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="370"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="714"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="496"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="716"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="754" mass="86291" checksum="20D835213FC3B361" modified="2014-04-16" version="1">MSKTKSSESVKVVVRCRPMNEKEVAANFERMVSVDVKLGQVSVKNPRGAPSELPKTFTFDSVYDWNSKQVDLYDETFRPLVDSVLLGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQAKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGLDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANIGPASYNVEETLTTLRYSNRAKNIKNKPRVNEDPKDALLREFQEEIARLKAQLEKRSGKRRRKRHRRHVGEGGEELGEEEEEEEEEDGEEGTEDDKDDTDYWREQQEKLEKEKKAIMEDHSLIAEEKLKLLKEKEKKMDDLKREKEAGEMLAAKVKAMESKLLVGGKNIVDHTNEQQKILELKRQEIAEQKRREREMQQQMESRDEETLELKETYSSLQQEVDIKTKKLKKLFAKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLIIENFIPMEEKNKIMNRAFLDDEDDHWKLRPITRIEDNQQMMIRPISAVGYKRPLSQHARMSMMMRPEARYRAENILLLELDLPSRTTRDYEEPAIAPKVAAALEAALQDEAEIQVDASTFESFGNRKLKSRLKTGKKASTPTSSSSPGSAGSPLYPQSRGLVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFB9</accession>
    <name>W5MFB9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA damage-inducible transcript 3 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">C/EBP zeta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">C/EBP-homologous protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">C/EBP-homologous protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">CCAAT/enhancer-binding protein homologous protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018786">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFB9"/>
    <dbReference type="FunCoup" id="W5MFB9">
        <property type="interactions" value="673"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007078"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007086.1">
        <property type="protein sequence ID" value="ENSLOCP00000007078.1"/>
        <property type="gene ID" value="ENSLOCG00000005864.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006305"/>
    <dbReference type="HOGENOM" id="CLU_993784_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFB9"/>
    <dbReference type="OMA" id="MVNLHKA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005864">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990622">
        <property type="term" value="C:CHOP-ATF3 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990617">
        <property type="term" value="C:CHOP-ATF4 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036488">
        <property type="term" value="C:CHOP-C/EBP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006983">
        <property type="term" value="P:ER overload response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046890">
        <property type="term" value="P:regulation of lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016670">
        <property type="entry name" value="DNA_damage_induc_transcript_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16833">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 3 DDIT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16833:SF0">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0346">Stress response</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="16" id="KW-0834">Unfolded protein response</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="BZIP" evidence="23">
        <location>
            <begin position="198"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="120"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="262" mass="28730" checksum="D8913989B19F6BA0" modified="2014-04-16" version="1">MTAESMTFSPYPPGVGPLCGAELEAWYEDLQDILFSDTGDLKSAQPDHCHEKEQEFLDMLETCSLAWLTDGQLWAQGEEQLCAAEPPLPAGAGGDLLPPEFFELLSEGAGETAVAEGEGALEIAGRLQGHPKEEEEEEEEKEEEAEQQQQQQYEEEKNHPHPSPASSEEDPPSPAESGSPLLPPSVSPQGGRGLAGHKRRGPQSSGAGASKKSRKERELENEQRAQELAEQNEQLKAEIERLSEEVQRTRQALIEKLVNARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGL3</accession>
    <name>W5MGL3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Collagen, type V, alpha 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214430.1">
        <property type="nucleotide sequence ID" value="XM_015358944.1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007522"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007530.1">
        <property type="protein sequence ID" value="ENSLOCP00000007522.1"/>
        <property type="gene ID" value="ENSLOCG00000006192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697959"/>
    <dbReference type="KEGG" id="loc:102697959"/>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155675"/>
    <dbReference type="InParanoid" id="W5MGL3"/>
    <dbReference type="OMA" id="WWTSRSP"/>
    <dbReference type="OrthoDB" id="8939548at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006192">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005588">
        <property type="term" value="C:collagen type V trimer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000001">
        <property type="entry name" value="Collagen alpha-1 type I chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000013">
        <property type="entry name" value="Collagen, type I, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1108">
        <property type="entry name" value="EMI DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867761" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="14">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="15">
        <location>
            <begin position="1267"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="98"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="706"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="772"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="824"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1069"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1123"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1207"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1502" mass="145423" checksum="A3BB4ACCA849E934" modified="2014-04-16" version="1">MMSFVHSRTFLFLAVSVAQVLFVKCQGGVSGDDLSCTENGQVYTNRDIWKPGPCRICVCDSGAILCDEIQCDEITNCEKPVIPDGECCPICQGNDNPTTHGGNGGRSYKGQKGEPGDVPFITGIRGRPGPMGPPGAQGYRGAHGPKGRPGPRGPPGFDGEPGVPGQPGEPGPPGHPTHPGLDSNHFASHMAGGFDEKSGLAGQVGMVPGTMGQAGARGPPGPPGQPGPAGPQGAPGESGEPGHMGPIGQRGPEGPPGKPGIDGEPGKSGQTGEIGFPGSPGARGFPGAPGLPGIKGHRGHQGPAGSRGEPGAAGSKGEPGPTGPMGGPGPQGPRGIPGERGRSGPTGPPGMRGPVGNVGKPGPMGPLGISGPPGFPGNPGLKGDAGPTGVRGPEGPQGQRGETGHQGKSGAPGLQGPTGTDGGPGAKGPAGTVGPSGPHGLLGPPGPPGPQGSTGPPGIKGQLGDTGVPGFKGEAGPKGEPGPPGAQGSIGPQGEEGKRGPRGDPGSVGPPGPVGERGAPGNRGFHGADGLPGPKGANGDRGPTGSSGPKGALGDPGRTGEPGLPGATGLTGNPGVQGAEGKPGPVGPSGEDGRPGQAGSIGTRGLPGIMGLPGPKGFNGDPGKTGEQGSAGVPGQRGPPGKDGEVGPAGPGGPAGPAGERGEQGPPGLTGFQGLPGPPGPPGESGKPGDQGIPGDAGAVGQIGPRGERGIPGERGELGAHGLQGPKGIPGAPGPDGPKGSPGPTGALGDPGPPGLQGMPGERGISGPPGPKGDRGASGEKGSEGTPGNDGARGLPGPLGPPGPHGPSGEKGEPGPKGPPGPPGSRASPGSRGEPGPTGPVGFAGPPGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSHGPPGPPGVAGLKGGRGTQGPPGVTGFPGPAGRVGPPGSTGPVGQPGPLGPPGKEGPPGLRGDTGPPGRHGERGPAGPPGSPGDKGDSGEDGPTGPDGPPGPPGTTGQRGIVGLPGQRGERGMPGLPGPSGPPGKQGATGPTGDKGPSGPVGLPGANGPAGDPGPDGPPGPDGPPGRDGVLGARGDRGDPGPAGLPGSQGAPGSPGPVGPTGDPGKRGDSGSRGPVGPPGTAGKRGLPGPQGPRGDKGDIGDQGERGQKGHRGFTGLQGLPGPPGTTGEQGAAGIVGPHGHRGPPGPVGPPGKEGYIGHPGPMGPPGSRGISGEIGPEGPPGDPGPPGPPGSPGPPTAAMEDFLDTQDYDVGPPPPPEFSEDEALPNSNKTDSFKTDPGVHATLKALSSQIDSMRSPDGTKKNPARTCEDLKQCYPQKKSGEYWVDPNQGCAEDAIKVYCNMDTGETCISANPSSIPRKTWWGTTNPTDYKPIWFGANMNGGAHFTYGNKDQSTNSVTVQMTFLRLLSKEASQTITYHCKNSVGYKDEKTGNLKKAVILKAANDLDLKAEGNSRFRYTVLEDTCYKANGEWGKTVFEYRTQKTARLPIVDIAPVDIGGSDQEFGIDIGPVCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHC5</accession>
    <name>W5MHC5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Single-strand-selective monofunctional uracil-DNA glycosylase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHC5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007784"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007793.1">
        <property type="protein sequence ID" value="ENSLOCP00000007784.1"/>
        <property type="gene ID" value="ENSLOCG00000006445.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT20">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004897"/>
    <dbReference type="HOGENOM" id="CLU_071760_2_0_1"/>
    <dbReference type="InParanoid" id="W5MHC5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006445">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017065">
        <property type="term" value="F:single-strand selective uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000017">
        <property type="entry name" value="Single-strand-selective monofunctional uracil-DNA glycosylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039134">
        <property type="entry name" value="SMUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13235">
        <property type="entry name" value="SINGLE-STRAND SELECTIVE MONOFUNCTIONAL URACIL DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13235:SF2">
        <property type="entry name" value="SINGLE-STRAND SELECTIVE MONOFUNCTIONAL URACIL DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="268" mass="28477" checksum="C5F005A082CC53B1" modified="2014-04-16" version="1">RGDQAVSLALPPAAAGLFEGTTAASRLLRAELELCARLRGLAFGEPVRYVYNPLEYAWEMHSCYVNTYCHDGQDVLFLGMNPGPFGMAQTGVVFSVPSLVDFCRAAHTDTHRHALSHPQPRGSKVVRSAKKGVRGRGGGRGVVFQPTGTARSIHTLNWVQMGVHAQKAASGRQLRQQGDSRAASGARSRLTGGSAAASVVFVAVAGGTAGTLLRKAQRRAPRRALGAAGMEVRVEGLMHPSPRNPLANKGWEGIARARLEELGILALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHJ9</accession>
    <name>W5MHJ9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01007882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007883">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007858"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007868.1">
        <property type="protein sequence ID" value="ENSLOCP00000007858.1"/>
        <property type="gene ID" value="ENSLOCG00000006499.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1959">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234638"/>
    <dbReference type="HOGENOM" id="CLU_004690_2_0_1"/>
    <dbReference type="InParanoid" id="W5MHJ9"/>
    <dbReference type="OMA" id="FIWRPSK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006499">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1360:FF:000009">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017851928" description="Alpha-mannosidase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="10">
        <location>
            <begin position="373"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="936" mass="105586" checksum="D4A7C7EF99B3C0BA" modified="2014-04-16" version="1">CSLKMAAGAASVLLLLLLVTGTRGFPARSPRHDPPGCGYQSCPATRPSMLNVHLVPHTHDDVGWLKTVDQYYYGARNDIQHAGVQYILDSVIEQLLADPRRRFIYVETAFFYRWWRQQGAQMRATVTQLVQQGRLEFVNGGWCMNDEASTHYSAIIDQMTLGLRFLNDTFGPCGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKRRRMKDKEMELLWRASESLQPPGADLFTGVLPNGYNPPAGFCWDQSCSDPPIQDDPQLEDYNADIVVKNFLDVAHNQAEMYKTNHIIMTMGSDFQYENANLWYKNLDKLIRYVNVQQSVGSNVNVLYSTPSCYLQELHRANLSWSVKRDDFFPYADGAYNFWTGYFTSRPALKRYERLSNNVLQACNQLEVLGGPSSRKGPYGDGDSDALREAMAVAQHHDAVSGTEKQHVANDYARRLAMGWARCQVLVSNSLSALSRSVTPRFYCENLNISVCPASENNSQFSVTIYNPLARSVSWTVRLPVNGTGYRVTDPRGQAVDTQVRVVWTAALTLLLSLRCVCLDSGTDSPPLAQVCVXLSQGFYWYNASSGNEDSPQTSGAYIFRPNSSTPFALCHRARARTLQTPLLTEVYQEFSPWVSQVVRLSRGQRQLELEWTVGPIPVEDGLGKEVITRFDSPMNTSGLFYTDSNGREILERRRDFRPTWDLKQTEPVAGNYYPVNTRIYMKDKQSQLTVLTDRSLGGSSIRDGSLELMLHRRLLYDDFRGVGEPLSEPGDYYDGLVVRGKLLVSLTPPGTAADTHRPEAERQALQPLLSLQPGESETGTLLEFSGLQAELPPAVHLLTVSQWDSDSVLVRLEHQFEKGDSQTHSQPVTVDLQKLFSTLEVVGVSEMSLAANQWKEDMRRLDWRGDAEWEPLHGRPGDPSAAVVRLQPMEIRTFLLRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI46</accession>
    <name>W5MI46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted frizzled-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Frizzled-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">FrzB-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636629.2">
        <property type="nucleotide sequence ID" value="XM_006636566.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI46"/>
    <dbReference type="FunCoup" id="W5MI46">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008055"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008065.1">
        <property type="protein sequence ID" value="ENSLOCP00000008055.1"/>
        <property type="gene ID" value="ENSLOCG00000006669.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683261"/>
    <dbReference type="KEGG" id="loc:102683261"/>
    <dbReference type="CTD" id="2487"/>
    <dbReference type="eggNOG" id="KOG3577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160494"/>
    <dbReference type="HOGENOM" id="CLU_058446_1_0_1"/>
    <dbReference type="InParanoid" id="W5MI46"/>
    <dbReference type="OMA" id="NAERCKC"/>
    <dbReference type="OrthoDB" id="5946121at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006669">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07441">
        <property type="entry name" value="CRD_SFRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03581">
        <property type="entry name" value="NTR_Sfrp3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000010">
        <property type="entry name" value="secreted frizzled-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000005">
        <property type="entry name" value="secreted frizzled-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035813">
        <property type="entry name" value="NTR_Sfrp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041759">
        <property type="entry name" value="SFRP3_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF97">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866153" description="Secreted frizzled-related protein 3" evidence="15">
        <location>
            <begin position="24"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="16">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="17">
        <location>
            <begin position="186"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="34"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="35735" checksum="0BFCD62E679E4E80" modified="2014-04-16" version="1">MLSYKVISFLAITCLIGIPRARAAACEPIRIPMCGSMPWNMTKMPNHLHHSTQANAILAIEQFEGLLGTNCSPDLLFFLCAMYAPICTIDFQHDPIKPCKSVCERAKCGCEPVMKKYNHTWPESLACEDLPVYDRGVCISPEAIVSAEGPDNPYYQEFNKCIPESMPDFPMDSHNGNCRGTNNERCKCEQIKLVEKTYLRKNYNYVIRAKVREIRSQRHDVSAVVEVKDVLKSSLVSIPRDIVTLHSNSGCLCPPLNANEEYIIMGYENEERSRLLFIEGSFAQKWKDRLGVKVKRWDQKLRKRNNMKKKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJR8</accession>
    <name>W5MJR8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 protein epsilon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJR8"/>
    <dbReference type="FunCoup" id="W5MJR8">
        <property type="interactions" value="1940"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008627"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008637.1">
        <property type="protein sequence ID" value="ENSLOCP00000008627.1"/>
        <property type="gene ID" value="ENSLOCG00000007116.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282547"/>
    <dbReference type="HOGENOM" id="CLU_058290_0_2_1"/>
    <dbReference type="InParanoid" id="W5MJR8"/>
    <dbReference type="OMA" id="KGCQLAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007116">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050815">
        <property type="term" value="F:phosphoserine residue binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10020">
        <property type="entry name" value="14-3-3_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="7">
        <location>
            <begin position="34"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="285" mass="32517" checksum="692149EC0799EDC0" modified="2014-04-16" version="1">EPPIDSRRLARRRAASPQPVQTPPRRLRADMGDREDLVYQAKLAEQAERYDEMVESMKKVAGMDLELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIRDYRQTVETELKSICNDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJS3</accession>
    <name>W5MJS3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox D11a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJS3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008632"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008642.1">
        <property type="protein sequence ID" value="ENSLOCP00000008632.1"/>
        <property type="gene ID" value="ENSLOCG00000007133.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161308"/>
    <dbReference type="HOGENOM" id="CLU_079662_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJS3"/>
    <dbReference type="OMA" id="CNFFTSV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007133">
        <property type="expression patterns" value="Expressed in intestine and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060272">
        <property type="term" value="P:embryonic skeletal joint morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000166">
        <property type="entry name" value="homeobox protein Hox-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
        <property type="entry name" value="DUF3528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="233"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="235"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="161"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="307" mass="35467" checksum="6C6D86B2C0228CFB" modified="2014-04-16" version="1">PHAYSAIDGQSLTHSTLLLTKREEEMTEYDDRSNCASNMYLPSCTYYVSAPDFSSVSSFLPQTTSCQINFPYSSNITQVQPVREVPFRDYALDHPTKWPYTSSFASYYSADEIMHRDLLQSPSRTEMIFKNDSVYSHHGSSNPSGNLFTSVGRNGILPQGFDQFFETGNTHPEKSNSDHFKQKTEPSFPGDAGSNRLTSESSQQKKQDPAENIEDDSSSSNCAEEKNIASSSLKSRKKRCPYSKYQIRELEREFFFNVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLNRDRLQYFTGNPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK28</accession>
    <name>W5MK28_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK28"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008737"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008747.1">
        <property type="protein sequence ID" value="ENSLOCP00000008737.1"/>
        <property type="gene ID" value="ENSLOCG00000007220.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4222">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000054197"/>
    <dbReference type="HOGENOM" id="CLU_912031_0_0_1"/>
    <dbReference type="InParanoid" id="W5MK28"/>
    <dbReference type="OMA" id="MNCKHEQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007220">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:004846">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002349">
        <property type="entry name" value="CD4-2 molecule, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422:SF6">
        <property type="entry name" value="HEMICENTIN-1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868310" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="33900" checksum="BE455D3D591B23EA" modified="2014-04-16" version="1">MMRGEICPLLCLGVFLHYAYADVFYAVINSRNLDLPCGVDTDTPDIQWKYRTPGSQEYTLILSTNTRSGATRKGQGTHLQSRTRINGRGLRISSVQKGDSGFYRCDQGGRQTEHQLLLVVVSADPPSPLLQSSKVILHCQVEGGSSPRRKWLWPNGTDAQSWGEGDMEMKGVSHTHHGAWKCQIEDNAFEATLNIYVLGIKPLTQPVLRIKEGDTVVLPCQLSASVSNMLSHLSFIRGGWKIESSQHPLLSLRSTGETGLCWNETKLKGIQNISQEKLYTDLSISLNEVSIYPCLILNLSNFSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKY1</accession>
    <name>W5MKY1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Myotubularin related protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKY1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009040"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009051.1">
        <property type="protein sequence ID" value="ENSLOCP00000009040.1"/>
        <property type="gene ID" value="ENSLOCG00000007446.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157272"/>
    <dbReference type="InParanoid" id="W5MKY1"/>
    <dbReference type="OMA" id="FNEAFLX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007446">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13341">
        <property type="entry name" value="PH-GRAM_MTMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14586">
        <property type="entry name" value="PTP-MTMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035888">
        <property type="entry name" value="MTMR3_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046352">
        <property type="entry name" value="MTMR3_PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF66">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="6">
        <location>
            <begin position="155"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="582"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="671"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="743"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="832"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="911"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="743"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="753"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="920"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="3">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="325"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="350"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="412"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1198" mass="133888" checksum="F16C88B0CF6CF840" modified="2014-04-16" version="1">MDEEGQQSLECIQANQIFPRKHPVLEEDNLQVPFPELHGEFTEYVGRAEDAIIAMSSYRLHIKFKESVVNVPLQLIESVECRDMFQLHVTCKDCKVIRCQFSTFEQCQEWLKRLTLAIRPPSRLEELFSFAFHAWCVDVYAGEKEQHGELCRPGEHVTSRFKNEAERMGFDLQNAWRITDLNNKYRLCASYPQQLVVPAWITDKELENVAGFRSWKRFPVVVYRHQSTGAVIGRCGQPEVSWWGWRNADDEHLVQSIAKACAVDCNSRKHLANGSCSRDYSNGADLSDGDFDASMTNSSEVESLAIQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMANIHSIRKSFQSLRLLCTQMPDPANWLSALEGTKWLQHLSVLLKSALLVVNALDRDHRPVLVHCSDGWDRTPQIVALSKLLLDPYYRTIEGFQVLVETEWLDFGHKFADRCGHGENSDDLNERCPVFLQWLDCVHQLQRQFPCSFEFNEAFLVKLVQHTYSCLFGTFLCNSVKEREDRHIHERTCSVWSLLRPANRAFRNMLYSSHSETVLHPVCHVRNLMLWSAVYLPNSSPTTPSDESCAPYPAPGSNPEDQPLGRLPKTRSFDNLPSACEAGGCPAPNRRSSDPSLNDKWQDHRRSLEINIGVGPDGATVQDQEARVNNLQEPGHQGGSPDGEAEEGEESVGGSVLGVDLKDREAKWVQQLVDENKEEAELSVAVGVAEGQMENILQEATKEETGIEDAQRELNSSAPGDSATLPTNVEPLPAVPVSDGKEGIESLEEKVEYTTVHLSQELQELPASDVHKLPDVSSVVFRTMANGYSQTCLGDQGVKESRGTSPQPSSPLSLPLDRPDDRTSLMESSTETLTEEVARQDVSGPSSACSLSNLPNEVECLVPCPKGEAGWEAEAERGTPKTSNVSKRPSLSALHPVPPEVSRDGAICNGDVSDGDPCGPQLPRHNGERAPLSRQVSAASCGSLNLHPRGSCSMGSQHRCLHALLGRPPPSPEQPARSHLDDDGLTLYNDIIQQRLRQIEAGHQMEVETLKKQVQELWSRLESQHLNNSLRINGEMGDEVTSIPDSECNLDPNCLSRCSTELFSEASWEQVDKQDTEVTRWYPDHLAAQCYGCESRFWLATRKHHCRDREPVDEAWHGIKMISLKSKQLIVNFNDELWQCVLLHLLRSEDPGPQSAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNC1</accession>
    <name>W5MNC1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027209">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009880"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009892.1">
        <property type="protein sequence ID" value="ENSLOCP00000009880.1"/>
        <property type="gene ID" value="ENSLOCG00000008141.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S41A">
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_766274_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNC1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
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        <property type="entry name" value="FSIP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47315">
        <property type="entry name" value="FIBROUS SHEATH INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47315:SF3">
        <property type="entry name" value="FIBROUS SHEATH-INTERACTING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="41147" checksum="9EEF43A03521EBC9" modified="2014-04-16" version="1">MASRDVKHREGVRPLAGTEVADFLLDQPLGSKTPIVPGVRTTFYTTSLCERLHQDSADFVLTDPSGRLLHSTYNSLHDPNLKQFFSQRDRQRLLVRQGLLTPQKKVICTTKEFNQYREYLTTVKLDWEKSFLREQKELVRKFLVLQEKKQIPPGITVCHLREWLLDCGSAARIWRARNGRTENQNTHSYSELAWRERLRQQLQELEVEVHRELRLERRWDKKKAPKQDMRLAAVPEEARVHSEAVSQTETHRSSALPSRATETSSSPGETSCPSEPSQKSQSTHLTHLKTSIEEQLRQRVSAEGLGPILGGLVRWVVTAVSDILIPAIQEFEESPGCPSDTSLQCCASGPGSRRELSPPRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPD0</accession>
    <name>W5MPD0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zyxin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MPD0">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010239"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010252.1">
        <property type="protein sequence ID" value="ENSLOCP00000010239.1"/>
        <property type="gene ID" value="ENSLOCG00000008407.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154273"/>
    <dbReference type="InParanoid" id="W5MPD0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008407">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09435">
        <property type="entry name" value="LIM3_Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000057">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212:SF1">
        <property type="entry name" value="ZYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212">
        <property type="entry name" value="ZYXIN/TRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="452"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="513"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="28"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="582" mass="63840" checksum="AF39EE1B57EBBD1B" modified="2014-04-16" version="1">MSDSSKPVMVSSTLTFNVSAPSFYNAPKKFASVAPPRPKLSSTQSQPLASHTSVASTAVIGRVGELPPPPPAVTEEFPPPPPPLDDSDLPAPPPEYQTTPPASEAPPPAFPEPPPPPPPEDIPSPTGAESSLPPPPPPPPPLPPACNAPVSAPQFEKIQWTITLLRTLYHLLELLCNYCYYNALSLSLKSSVAGSGAPLSMREVEELEKLTQRFIKDMENPPQIHAPSTGQHRVCSTAEQAFSHVVQSRSGLVLVCLFPADLSLISVENYISHSSSSSEXVLPHPCRTGWGSEILKDTARDFSVCVRACATSARVGGEKNQKVTRESWASLRFVILCLGSSRSLRPRFPERWSPLPVSHARLAGTASYWFLRHLGRESAPAFSDPKFKRRPAVETSLDYSCFALDDLLNLSHMEATYKTHFCRTDYRAVRGLLHSHLGGTPQCEDCYTSSLEQCSHCKEKISDRVLRAVGRSFHAHCFRCVQCGCSLEGAPFITDDADQPYCVPDYHRRFSPQCVSCKEPIVPDPGSEETVRVVALDKNFHLKCYRCEDCARPLSIEADSDGCYPLDGRILCLKCHTHRAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR88</accession>
    <name>W5MR88_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein fem-1 homolog C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FEM1-gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a CRL2 E3 ubiquitin-protein ligase complex, also named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the fem-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR88"/>
    <dbReference type="FunCoup" id="W5MR88">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010913.1">
        <property type="protein sequence ID" value="ENSLOCP00000010897.1"/>
        <property type="gene ID" value="ENSLOCG00000008945.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158626"/>
    <dbReference type="HOGENOM" id="CLU_020042_2_0_1"/>
    <dbReference type="InParanoid" id="W5MR88"/>
    <dbReference type="OMA" id="FMTTEWR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008945">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000104">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000076">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000163">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000173">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF14">
        <property type="entry name" value="PROTEIN FEM-1 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="500"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="546"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="636" mass="70621" checksum="390C8D90E82FFAE4" modified="2014-04-16" version="1">CIDLSVSHVSIFVLPLRNEMDLKTAVFNAARDGKLRLLQKLLENKAGDEVSKLMAEKTNGATPLLMAARYGHLDLVEYLLECCLAPVEVGGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLGHGASVNNTTLTNSTPLRAACFDGHLDIVKYLVEHKADLEVANRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLEIMKMLLKYGAKMEKDGYGMTPLLSASVTGHTNIVDFLTQHQQTSKMERINALELLGATFVDKKRDLLGALKYWKKAMDMRYSESGNILYKAEPKHLIMAYDYAKEVNCADELDNLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTSVSFDDLMGILSKSVLEIERAVKQTQPPPDPAQLSKALSIILHLICLLEKVPCSVEQDHFKKETIYRFLKLRPSGKNGFTPLHLAVDRNTTCVGRYPVCKFPSYQVASILVECGADVNCRDEDNNSPLHVAAFNNNPDIMNLLIACGSHFDSTNSFKQTACDLLDEKEMAKNLIQPINHTTLQCLAARVIVKHSLVYRGNIPQKLEAFVLLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSS1</accession>
    <name>W5MSS1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 12">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSS1"/>
    <dbReference type="FunCoup" id="W5MSS1">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011430"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011447.1">
        <property type="protein sequence ID" value="ENSLOCP00000011430.1"/>
        <property type="gene ID" value="ENSLOCG00000009363.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157399"/>
    <dbReference type="InParanoid" id="W5MSS1"/>
    <dbReference type="OMA" id="GSEWCIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009363">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12774">
        <property type="entry name" value="RRM2_HuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12656">
        <property type="entry name" value="RRM3_HuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000017">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034918">
        <property type="entry name" value="HuD_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="88"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="174"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="325"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="408" mass="45148" checksum="5E5F0475F69BEDF9" modified="2014-04-16" version="1">QYHICNSFGMLLFTSEGSTETQWRQAELPQLQGWAEKGMLTQPKMIISNMEPQVTNGPTATTANGPSSNSRSCPSPMQTGSSTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGASRGVGFIRFDKRIEAEEAIKGLNGQKPSGAAEPITVKFANNPSQKTSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDSMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTK7</accession>
    <name>W5MTK7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Embryonic ectoderm development</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTK7"/>
    <dbReference type="FunCoup" id="W5MTK7">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011716"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011734.1">
        <property type="protein sequence ID" value="ENSLOCP00000011716.1"/>
        <property type="gene ID" value="ENSLOCG00000009588.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047334"/>
    <dbReference type="HOGENOM" id="CLU_032683_1_0_1"/>
    <dbReference type="InParanoid" id="W5MTK7"/>
    <dbReference type="OMA" id="RDVHRNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009588">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045685">
        <property type="term" value="P:regulation of glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000056">
        <property type="entry name" value="Polycomb protein eed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="441" mass="50353" checksum="370289044E99A39F" modified="2014-04-16" version="1">RKMSESQAEAGNEMPSKKQKLSSDENSNPDLSGDENDDAISIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHGQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTFDSSTSHPLLAVAGSRGIIRVINHITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADFDLLGEKIMSCGMDHSLKLWRINSERMQKAIRGSYEYNPSKTNRERPFVSQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDRIRANESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHPKCNAAIRQTSFSRDSSILIAVCDDASIWRWDRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTR6</accession>
    <name>W5MTR6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 39A</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM39 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639253.1">
        <property type="nucleotide sequence ID" value="XM_006639190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTR6"/>
    <dbReference type="FunCoup" id="W5MTR6">
        <property type="interactions" value="1238"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000011793.1">
        <property type="protein sequence ID" value="ENSLOCP00000011775.1"/>
        <property type="gene ID" value="ENSLOCG00000009633.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686290"/>
    <dbReference type="KEGG" id="loc:102686290"/>
    <dbReference type="CTD" id="55254"/>
    <dbReference type="eggNOG" id="KOG3828">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018895"/>
    <dbReference type="HOGENOM" id="CLU_028992_0_0_1"/>
    <dbReference type="InParanoid" id="W5MTR6"/>
    <dbReference type="OMA" id="RFKQLIF"/>
    <dbReference type="OrthoDB" id="5862608at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009633">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901097">
        <property type="term" value="P:negative regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019397">
        <property type="entry name" value="Uncharacterised_TMEM39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12995">
        <property type="entry name" value="FI21814P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12995:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 39A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10271">
        <property type="entry name" value="Tmp39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="481" mass="54993" checksum="7783DF15014DF38A" modified="2014-04-16" version="1">MPGGRRGPSRQQLSRSALPSLQTLVGGGCSNGTGLRNRSSNSVGLSAPPITALITPEPVRHSRIPELPLDGSLLFEFLLFTYLLVALFVQYINIYKTVWWYPYSHPAASTSLNFHLIDYHLATFITVMLARRLVWTIISEASQTGPTSVVRYTVLIAARLTLLTLCGWVLCWTLVSLFKNHSVLNLLFLGYPFGVYVPLCCFHQESRTQTLPADCGYLEQEQMDGPLLRPKDFLTLLRENLREQFNSPTSIPAHTCPLSPDLIRNEVECLKADFNRRIKEVLFNSLFSAYYVAFLPLCFVKSTQYYDMQWSCEHLIMVWINAFVMLMSQLLPPKYCDLLHRSAAHLGKWQKLEHGSYSNAPQHVWSENTIWPQGVLVRHNRCLYKAVGPCNVAVPSDVSHARFYFLFHKPLRILNLLILIEGSVVLYQLYSLLRSEKWNHTLSLALILFCNYYVLFKLLRDRIVLGKAYSYPLHSFGIKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUB5</accession>
    <name>W5MUB5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Troponin I type 1a (skeletal, slow)</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUB5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011974"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011995.1">
        <property type="protein sequence ID" value="ENSLOCP00000011974.1"/>
        <property type="gene ID" value="ENSLOCG00000009801.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="HOGENOM" id="CLU_098686_1_0_1"/>
    <dbReference type="InParanoid" id="W5MUB5"/>
    <dbReference type="OMA" id="KQDFRAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009801">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="troponin I, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF9">
        <property type="entry name" value="TROPONIN I, SLOW SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="22061" checksum="526844E45FBD6C1D" modified="2014-04-16" version="1">KPSELQEKRKSKITASRKLMLKSLLLAKAKEELEQEIVDKEEEKERYLSERVPPLQLHGLSYNQLQELCQELHAKIDVVDEERYDIEAKVIHNTREIQDLNMKVFDLRGKFKRPALRRVRVSADAILRSLLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKPGTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUE9</accession>
    <name>W5MUE9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LON peptidase N-terminal domain and ring finger 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027704">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUE9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012008"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012029.1">
        <property type="protein sequence ID" value="ENSLOCP00000012008.1"/>
        <property type="gene ID" value="ENSLOCG00000009823.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033329"/>
    <dbReference type="HOGENOM" id="CLU_013989_1_2_1"/>
    <dbReference type="InParanoid" id="W5MUE9"/>
    <dbReference type="OMA" id="HDTGTEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009823">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16513">
        <property type="entry name" value="RING-HC_LONFs_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16514">
        <property type="entry name" value="RING-HC_LONFs_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF6">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="295"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="6">
        <location>
            <begin position="374"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="585" mass="67558" checksum="51B4F39E0F62A488" modified="2014-04-16" version="1">MDLLLCPTCHCLMSEPVTVSCGHSFCKSCLGTILPSRCQICRERLKLRDVKNIKTNVLLFSVMEKCYPEDTKLKCNLQDRLKNCEFTEALRIIDEGMRLAPNDVSLQISRAEAKLGLQRYSDALKDLDLLCEMRPTWTEVFFRKGNIFLEMGRKIEALIQYHHCLRLQHEFAPAKHEIQKILHSNGESVPEEVEDLLKVTSMYLRNACPAADPVLSEDAPLSQPLEEERVQASGETNKAKKADSCVSLSHSVSLLPARKNEEELMNRDTEHQRSEICSDVQEFPLNMLSPSDFECPLCIRLFFEPVTTPCGHTFCKSCIERSLDHNLRCPLCKQPLQEYLKNRKYNRTILLEDIMSRLFPCELAERKQIHDAEIAELSNLTKDIPIFVCTVAFPGVPCPLHIFEPRYRLMMRRCMETGTKKFGMCTYEHGKGFADYGCILEILGLDLLPDGRSYVDTIGRNRFRVTKRGQRDGYHTADIEYLEDQKVEGAELELVQRLHDSIYQQACDWYRRLNPRIRDQINKQYGHMPEREDNIQASPSGPSWCWWLLSVMQLDPMYQTTVLSLTSLRDRLGHLRVVLEYFSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUI4</accession>
    <name>W5MUI4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUI4"/>
    <dbReference type="FunCoup" id="W5MUI4">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012043"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012064.1">
        <property type="protein sequence ID" value="ENSLOCP00000012043.1"/>
        <property type="gene ID" value="ENSLOCG00000009849.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159943"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="InParanoid" id="W5MUI4"/>
    <dbReference type="OMA" id="HHEIYAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009849">
        <property type="expression patterns" value="Expressed in brain and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031489">
        <property type="term" value="F:myosin V binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000206">
        <property type="entry name" value="Ras-related protein Rab-3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="25313" checksum="1DA23BA18118CBD9" modified="2014-04-16" version="1">LPFTQMAKVDSRFGHKDSSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFSNSFVSTVGIDFKVKTVYRNDKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDICNEESFNAVQDWATQIKTYSWDNAQVILVGNKCDMDDERVVPADKGKQLADQLGFEYFEASAKENINVRQVFERLVDIICVKMSESVDTDPAMVTGAKNTRLTDNPPQLPQNCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUS5</accession>
    <name>W5MUS5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUS5"/>
    <dbReference type="FunCoup" id="W5MUS5">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012134"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012155.1">
        <property type="protein sequence ID" value="ENSLOCP00000012134.1"/>
        <property type="gene ID" value="ENSLOCG00000009918.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240385"/>
    <dbReference type="HOGENOM" id="CLU_013137_0_3_1"/>
    <dbReference type="InParanoid" id="W5MUS5"/>
    <dbReference type="OMA" id="RFAYHIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009918">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="345"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="241"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="547" mass="63393" checksum="7B7FC12CECB68BA2" modified="2014-04-16" version="1">FSGMALSSNILSEEQLRCSVCLDIFDKPVTIPCGHNFCMVCIERSWENRVLCQCPLCKEIFNKKPDLRVNRSFAEITEQFKRTRPSSSEEFSVKPGEVLCDFCTTERLNAVKSCLVCLASYCDEHIKPHYQGTAFRRHRLIDPVNNLENRICKKHERCLELFCKTDQMCICQLCTETDHRSHNTVSAETEWLEKKAQLGETEVKIQQMIQYRRKKMEEIKISMEHSKSRACREVENSFQVFSDLIRSIERSQAELLELSEEKQKAAERLAESLIKELEDEITEIQRRHAELEQLSHTEDYIDFLQVSRFYLCKKSHKTCDLPIHTDLCLADLRRAVCQLEDRLSEEIEKLPEIKMRKMRQNAVDVVLDPNTAHPSLILSADGKQARHGDIRQVFHDNPERFDACVNVLGKDGFTSGKHYWEVQVDEKTEWTLGVASESIERKGKITLSPKKGYWTVGLRNSHEYKAFSRPSSVLLLSTKPRKIGVYVDYEKGQVSFYDAEARTHIYTFTDTFKEKMYPHFSPGAHEGGRNTAPLIISPINQADYQWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV95</accession>
    <name>W5MV95_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">EvC ciliary complex subunit 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001213">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV95"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012304"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012325.1">
        <property type="protein sequence ID" value="ENSLOCP00000012304.1"/>
        <property type="gene ID" value="ENSLOCG00000010056.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692603"/>
    <dbReference type="KEGG" id="loc:102692603"/>
    <dbReference type="CTD" id="2121"/>
    <dbReference type="eggNOG" id="ENOG502QUDD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154127"/>
    <dbReference type="InParanoid" id="W5MV95"/>
    <dbReference type="OMA" id="KDGQVWS"/>
    <dbReference type="OrthoDB" id="8910527at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010056">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026501">
        <property type="entry name" value="Limbin/EVC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16795:SF13">
        <property type="entry name" value="EVC COMPLEX MEMBER EVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16795">
        <property type="entry name" value="LIMBIN/ELLIS-VAN CREVELD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="790"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="991" mass="114604" checksum="87E9052C6442BF42" modified="2014-04-16" version="1">MDGLEGLTGCTSDVFIEFVESLQLPSLLLLTVAVVFGVIVGISTSTLFYVFLLKPLLLKRKFQGCDAKRLFETDDLDKEDNQSDCISIEKKQCQNTTLNDKERKLPLNSDVAAFALKAKVVYPINQRYRPLADGASNPSLHENTRLPMLPNQMQEDSSSSSLESLSQDNEDDSGQFISSSPMPKAFQNEKFIKVNRFPETLSFTGFDARISLYCLGIQKSEQLETELWQEKNVVFLQVLQILLNAWLRKEVIDNAFHKRCLQMQERELDTLQKHPSLKLSNTEQNEDPDTPYCTLEEMERNGKDKLECQIQMILGFNRQIESLCQHLQSKSSLHSDLVDRMTGSLIENLLLMENLLNESQYSDMRNIQEKVTWWEYTTCTLESKLWLLKQEAVSRLKFTAKVLEHLTSDGELSFHEMERILSDLQCRFKEEVQQATDECAKQTKDLVNEMTKKVDTRRKKLKKIQTKERQRIQHNIQQIIDPKEFIKAYHDLLVKQRKDCCELEDKQDMRITEAVCDLWKKLHFSASQKMVDIVKELFLGALPNQTQLPLDKCDVLRQEASHDLSLHLPTEEAHANHNLEVLNQHLEQEKQTWCEEEALARVTLKHLTEQQQKLLHGTLARQQGPHNGTEELIEMKHLLLLRAVNRQFAARQFRLRVLKEMRLSKLKTLLNETRLEFTKGLETEDCVKQHFNVPKGPSKESVGSEAERRLVQESQLIRLEFQQEFLSELSAAAELIQEHTALVIGHTLAQNARQQAAPQDLQENEHWKHQLTEAATESVYVTRESVSTIIRKYYAQIQNVTEALEQERQQRLQCIGDTHDNIKNQIDLRKCLQKELANWSKKPTSTEFYQRVEIQKKKMLTQYDSDLDSAFEILRQKKSVLDHMEKHLCGKLQEAEETFVAHLAALARASLPGADSLRKDNHSDDEQPLGKKMSSLLNEESSCSLCDSSHQRESSDLPRYEQGFNLDLFEDKKKMKKKGKTESEKIYSDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW46</accession>
    <name>W5MW46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01003563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW46"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012605"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012629.1">
        <property type="protein sequence ID" value="ENSLOCP00000012605.1"/>
        <property type="gene ID" value="ENSLOCG00000010274.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214434"/>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_1"/>
    <dbReference type="InParanoid" id="W5MW46"/>
    <dbReference type="OMA" id="QLCDFFG"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010274">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000177">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000033">
        <property type="entry name" value="glycerol kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="13">
        <location>
            <begin position="52"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="14">
        <location>
            <begin position="363"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="601" mass="65965" checksum="82006997410D5579" modified="2014-04-16" version="1">MVKGEAGLCPEGAGSGRIKTAGCPAQVTTTPDKALVHSRMAASDRIMLGPLVGAIDQGTSSTRFLVFNAKTAELLSHHQVEIKQNFPKEGWVEADPKEILQSVYECMERTCEKLSQLNIDISNIKAIGVTNQRETTIVWDKETGEPLYNAIVWLDLRTQSTVERLINKTPGRNKNHLKNKTGLPISTYFSAVKLRWLMDNVEEVHSAVLSGRAMFGTVDSWLIWCLTGGRQGGVHCTDVTNASRTMLFNIHTLEWDPDLCKYFDVPMDILPKVRSSSEIYGLMKISSSLRFSGCRLCLPSKGGFGFVNSAVKLSVCFLRTSAGSTHAYTVYLLYDTHFSIKISDLFWFQCYSLIPLAHACEQLEGSVAIAGAVVQWLRDNLGIIQSSGEIEKLAAKVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNRNHFAFAALEAVCFQTREILDVMNQDSGMPLTQLQVDGGMTANKLLMQLQADILCIPVVKPSMPETTALGAAMAAGSAEGVSVWSLVPEDLTAVTCERFEPQINPEESEYRYARWKKAVQKAMDWESTEPLSDGNGETSIFSSLPLGFYILGSMLMLIGAKSIAAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWE3</accession>
    <name>W5MWE3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RUN domain containing 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUNDC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5MWE3">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012702"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012726.1">
        <property type="protein sequence ID" value="ENSLOCP00000012702.1"/>
        <property type="gene ID" value="ENSLOCG00000010363.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159175"/>
    <dbReference type="HOGENOM" id="CLU_045987_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWE3"/>
    <dbReference type="OMA" id="KQWYDKS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010363">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047340">
        <property type="entry name" value="RUNDC3A_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251">
        <property type="entry name" value="RUN DOMAIN-CONTAINING 3 PROTEIN RUNDC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251:SF1">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="4">
        <location>
            <begin position="56"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="458" mass="52032" checksum="DADC1BBFEADD1632" modified="2014-04-16" version="1">RRRRRKLEMASLSLGLNLSRKKGASRNGAVERKNLITVCRFSVKTLIDRSCFDTIDDSSSEFVNFVSILEQILSHRLKGQITWFGYESPRGFWDYIRVACGRVPHNCLSSIESMENVRSSRAKFETWRNQMYIYENLLFSIFLTSSLKLIKILSRRFYEDGAIVLGEEANLLADMLIGLNAIDFSFCLKGEGIDDNRPAIIDYTPYLKFTQSSDSISSDEEELRTLGSSGSESSTPENMGPALFMDQSSWYNKCKRVEQKYRLALEQKGYLEELVRLREAQLSESVSQNKILLQRLEETDISHKLEKEQLEYIVLELQDQLTVLKNHDLRSRQELTAHLTNQWPSPGALDTKAVALDTLAYRKHTRQWKEKSFRSLEQLSAEISLSQTSLDPAHIQDLDSKQDESHSQSEGKEDTPSLLGLCGSLTSVASYKSLASLKSSECLASPITEMTSPGFTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXE7</accession>
    <name>W5MXE7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine rich repeat containing 74A</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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            <begin position="15"/>
            <end position="38"/>
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            <begin position="25"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50222"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="450" mass="49966" checksum="51F107C961E0C335" modified="2014-04-16" version="1">MAQSSLSVESLCLETQNGSPATDPDSGDEWDTDLELNDGKETRRSVSARELYLQACRLVGVVPVSYFLRNLGNTNLNLNHHGLGPQGAQALAIALVSNMTIITLELEDNCLFAEGTKYLAEMIKGSCTIQNLNLSNNHLKSAGAIYMSKMLLDNISLRTIKLSGNEFREEDAKCFTDALSNNYRVKELDLSHNEFSEKGGEHLGQMLANNEGLEMLNLSWNRLRMKGAVALCAGLKVNITLKYLDLSWNGFGNEGALALGEALKFNNTLLHLDLSNNRITNEGVGLLCKGLVANDTLKVLRMAYNSLTVEGALALVTAVKNTPKSALEEINISNVLVNEAFLHLLELTCQEHQALEVLYGGVGGFIAKKLPRRPDPMKVIQDYLDERKLRLWDFFRNIDKDGSMRVPVSDFRKAVQQSSIPLDRFQIEELVQRLDKDRSGNVDYSFLIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYA1</accession>
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        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000013388.1">
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        <property type="gene ID" value="ENSLOCG00000010897.1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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            <end position="25"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="33753" checksum="BA605DA2E6FF0E3A" modified="2014-04-16" version="1">QKMYSLVLSCVCAVLGIFGNSLVIAMKKKSKSRARFSRMLEIHIACGNTLHLVFRVLSVTVPMSLPFCYPMSGACKFVTSVMHTGRKVSVWCIVILSWYRFCKMKRTTHVSLWVSRLERSTKVSTFLAFHWLGWGLLYAPLFLIRETLGNNSTKTCICSVANVAGRESRFLVIYSTLYLPVLQYTAAVLMTAFNVKTLHFLFKCHAHVSSCSDTLARRELCREQGAAVTLLCLVCVYDTCVLLQSILTYGFIGTLSADIGRTVFDLYGTLIPYIILLGHQDVRLKLTSFWISDRAMGVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYE9</accession>
    <name>W5MYE9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibroblast growth factor receptor-like 1</fullName>
        </recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Has a negative effect on cell proliferation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013408"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156736"/>
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        <property type="component" value="Linkage group LG4"/>
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        <property type="entry name" value="Ig_sub"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890:SF10">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="12"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="133"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="234"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="483" mass="53620" checksum="124908081A0CA175" modified="2014-04-16" version="1">MIVFSDCARGPPRIAEKIIHRQTVRIGRTMKLLCPVEGDPPPLIMWTKDGRNIHSGWMRFRILRQGLKIKEVEAEDAGTFVCKATNGFGSVNINYTLIVIDDSSTGKDGPVLPEGGGTQYTNEDMSGKQWVRPRFTQPAKMRKRVIARPVGSSVRLKCAASGNPRPDIAWLKDSKPLTPQEVGEGRKKKWTLSLKNLAPEHSGKYTCRVFNRAGEINATYKVEVIPIERTKSKPILTGTHPVNTTVDYGGTTSFQCKVRSDVKPVIQWLKRVEYGNENKYNSTIDVGEHKFVVLPTGEVWSRPDGSYLNKLLITRAKEEDAGMYICLGANTMGYSFRSAFLTVLPDPKPPSTPVLTPPSSSLPWPVIIGIPAGAVFIFGTILLWLCQTKKHCSSPVATAASAQAAHRQPPRERTCVPQVPDKDCGNYEEYLAQQQHLLAQGGPALASAMAPKMYPKIYTDIHTHTHSHVDGKIHQHQHIHYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYL0</accession>
    <name>W5MYL0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myozenin 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myozenin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYL0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013469"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013498.1">
        <property type="protein sequence ID" value="ENSLOCP00000013469.1"/>
        <property type="gene ID" value="ENSLOCG00000010984.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S22C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183027"/>
    <dbReference type="HOGENOM" id="CLU_071316_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYL0"/>
    <dbReference type="OMA" id="WIQVMPE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010984">
        <property type="expression patterns" value="Expressed in muscle tissue and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051373">
        <property type="term" value="F:FATZ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031433">
        <property type="term" value="F:telethonin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
        <property type="entry name" value="MYOZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941">
        <property type="entry name" value="MYOZENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941:SF16">
        <property type="entry name" value="MYOZENIN 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05556">
        <property type="entry name" value="Calsarcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="29645" checksum="2325EE5B772B237A" modified="2014-04-16" version="1">SVQYHTIYMNNTGGGFQNSTYSSLAKERRDMAIALSKEVEGEHLNLGKKISVPQDVQMEELALLSNKGSRMFHERQKRVQRFTLENTGESRITVSPPDQHERCRTGAKHRRWSPKKQPGKGHFLSALKNTAATKPGSPSVIAPGYAGPLKEIPHEKFNITVIPKSYHSPWQKARGENDLLVNLNVQLPEVPQMPGPSTFRCFNRVAMPFGGTAGSERIFPLPGFELSQAQTEPNLNWERINNRPNFNRAPRGWGVNYSPESSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0P5</accession>
    <name>W5N0P5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 25">Receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3 25">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3 25"/>
    <dbReference type="EMBL" id="AHAT01017686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0P5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014204"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014233.1">
        <property type="protein sequence ID" value="ENSLOCP00000014204.1"/>
        <property type="gene ID" value="ENSLOCG00000011537.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155450"/>
    <dbReference type="HOGENOM" id="CLU_003384_1_1_1"/>
    <dbReference type="InParanoid" id="W5N0P5"/>
    <dbReference type="OMA" id="GYYYEWV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011537">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048408">
        <property type="term" value="F:epidermal growth factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05108">
        <property type="entry name" value="PTKc_EGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000001">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000002">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000044">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000005">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000006">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002828">
        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="3"/>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Egfr_JX_dom"/>
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        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
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        <property type="entry name" value="Furin_repeat"/>
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        <property type="entry name" value="GF_recep_IV"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Rcpt_L-dom"/>
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        <property type="entry name" value="RTK"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR049328">
        <property type="entry name" value="TM_ErbB1"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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        <property type="entry name" value="Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR016245">
        <property type="entry name" value="Tyr_kinase_EGF/ERB/XmrK_rcpt"/>
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        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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        <property type="entry name" value="TYRKINASE"/>
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        <property type="entry name" value="FU"/>
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        <property type="entry name" value="L domain-like"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 25 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 30" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867082" description="Receptor protein-tyrosine kinase" evidence="30">
        <location>
            <begin position="19"/>
            <end position="1210"/>
        </location>
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        <location>
            <begin position="650"/>
            <end position="671"/>
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        <location>
            <begin position="716"/>
            <end position="983"/>
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        <location>
            <position position="841"/>
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        <location>
            <begin position="722"/>
            <end position="730"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="749"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00004251"/>
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            <dbReference type="ARBA" id="ARBA00011902"/>
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    <sequence length="1210" mass="134910" checksum="3899B99A18BBF500" modified="2014-04-16" version="1">MQFEIAIVLSSLLTLGCCSIQDKRVCQGLSNKLTLLGTSQDHYNNIVRMYSNCTVVMENLEITYVEKDYDLSFLKSIREVGGYVLIALNKVSRIPIDNLRIIRGHILYQNSYALAVLSNFDKQSSVNKNQSSNVGLQELSLKSLTEILKGGVKFTGNPFLCNAETIQWYDIVDKSKNPEMFLIFANNTLNCKKCDPNCFNGSCWAPGPSNCQTLTKLNCAEQCSHRCKGPKPSDCCNEHCAAGCTGPRSTDCLACRDFQDEGTCKQVCPQLMLYNPNTHQLEMNPDGKYSFGATCVKSCPHNYVVTDHGSCVRTCTANTHEVDENGVRKCKKCEGPCPKACNGLGMGNLVNVLSINASNIDSFRNCTKINGDVSILPVAFRGDSYTKTPVLDPSKLDVFKTVKEITGFLLIQAWPENMTSLSPLENLEIIRGRTKQHGTVSVAAVNIDITSLGLRSLKEISDGDVVIRGNPHLCYTNADQWKRLFKLEKQNARVSENADPTECSALTQTCDELCTSEGCWGPGPSMCFSCQHFMRQQQCVNACNVLEGLPREFIMDKKCIECDPECMPQNGTQTCTGSGPDKCAECAHFKDGPHCVHKCPSGIPGENDTFIWKYADEKKVCQLCHPNCTQGCTGPGLAGCDHQTSQLSSIAAGVVGGLLVTVIIALAIFILMRRRYIKRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIQVLGSGAFGTVYKGLWIPEGEDVKIPVAIKVLREATSPKANKEILDEAYVMASVNNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVKEHKDNIGSQYLLNWCVQIAKGMNYLEERHLVHRDLAARNVLVKTPQHVKITDFGLAKLLSADEKEYHADGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEIAGILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPRFRELIAEFSKMARDPPRYLVIQGDERMHLPSPTDSKFYRTLIDGDEMDDVVDADEYLVPYNGFFNSPSTSRMQLLHSMSLNSSMGSCTIRNGQGYPMREDSFIQRYITDPTDGAFEKDFQPVPEYVNQNGNPVSDVMNPIYQDSVVPISAVTRAQNADSQYLNSFSNTADFHEYLNTVQPALPRPLFDNPHYCDQKVTHQTSLDNPDYQQDFFPPKSKVNGHLPAAENTEYLALSAQAEKYIYSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N259</accession>
    <name>W5N259_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Family with sequence similarity 193 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM193 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005450">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N259"/>
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        <property type="interactions" value="1511"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014718"/>
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        <property type="protein sequence ID" value="ENSLOCP00000014718.1"/>
        <property type="gene ID" value="ENSLOCG00000011965.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV04">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000973"/>
    <dbReference type="InParanoid" id="W5N259"/>
    <dbReference type="OMA" id="CPYSHTA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011965">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029717">
        <property type="entry name" value="FAM193"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031802">
        <property type="entry name" value="FAM193_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15109">
        <property type="entry name" value="AGAP004327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15109:SF3">
        <property type="entry name" value="PROTEIN FAM193B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15914">
        <property type="entry name" value="FAM193_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM193 C-terminal" evidence="5">
        <location>
            <begin position="865"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="495"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="701"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="495"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="923" mass="98504" checksum="474C26D14795235C" modified="2014-04-16" version="1">MARKKSKQQGAGNKELGTGQQSLAKSPVSPGDTAGGGGDAGLDRLAKPNQECCVLCHAKWVLSPQWYNGKKKNLSPHSSRAPAFNCAALRWARRFSTTGSVPSCRCQEWRQSSAWWLCQCQPRSATASVAKAMLPSPMQVSLSHSSCKSQSCGNGYPEQSSVEWDPSSFLSAHKLSGLWNSAHANGGAHCNHGAAAHPQQGGTEGPNCHEKRPSHEAPGKSAKIAGTKVCPYSHPASQNSSATAPGSPLSTSSDLCKTTPKHLKSMCRRPTPPGEAFHSTEHHQHSDLAVPPNSPTGLSSQHSSLLPPKQSSSQHVHGTSPSPGLTTHAPFTPLVPNLHVPVSKMVAGPDNPASLHKPGACKSSHIPAVNTQHAKLAPSPTGCGHPCNGHSNGTVTSPGAGHVSSGACRDQACKGHKLANGTGAACHPPCELEEGLGEDEDSSSERSSCASSSTNQKDGKYCDCCYCEFFGHNAPPAAPTSRNYAEIREKLRSRLTRRKEELPPRQDSDPAAPGAIDHRDVDELLDFINSTEPKPVNSAKAAKRARHKQKKKEKEKAQQCSAEPLHIDARSSPPGQPQEGAPAPETEAGRLLEWPQLELERVNSFLTSRLEEIKNTIKDSIRASFSMYDLNLDVNDFPKKAATLEGNHLLSHLNGSSDLQQIDLDLSPLTLGSLKNHLVNGREGLAAQDVQRLNATPSLSKLIRVRSPERSPGRNTSPVPSREGAALGVKSKEESPDAKAAGAAGKQRKGKKQQQQQQQAPAKAVKSATGSELQKNKETPARATGSAKRPEEGKVPRPGLGGPDSGGCRPPEKNGADSQETTGGARPDLEPEARVHFLTAAGSQQPRGKSKKSKSKMDKSSSSIDDVFLPKDLDGTEMDEIDREVEYFKRLRFCLDSAKQTRQKVAVNWSNFSLKKVPSNAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2A7</accession>
    <name>W5N2A7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01017243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014766"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014795.1">
        <property type="protein sequence ID" value="ENSLOCP00000014766.1"/>
        <property type="gene ID" value="ENSLOCG00000011999.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166813"/>
    <dbReference type="HOGENOM" id="CLU_000091_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2A7"/>
    <dbReference type="OMA" id="QHYMSGT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011999">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12881">
        <property type="entry name" value="SPRY_HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14401">
        <property type="entry name" value="UBA_HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000015">
        <property type="entry name" value="LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000006">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035768">
        <property type="entry name" value="SPRY_HERC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF6">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="371"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="421"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="476"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="529"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="580"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="632"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="683"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="15">
        <location>
            <begin position="1970"/>
            <end position="2161"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="3372"/>
            <end position="3405"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="3694"/>
            <end position="3735"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="3945"/>
            <end position="3993"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="3994"/>
            <end position="4048"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="4049"/>
            <end position="4100"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="4101"/>
            <end position="4152"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="4154"/>
            <end position="4205"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="4206"/>
            <end position="4257"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="4258"/>
            <end position="4309"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="16">
        <location>
            <begin position="4449"/>
            <end position="4796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1315"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1356"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1411"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2548"/>
            <end position="2582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2762"/>
            <end position="2785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1316"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1359"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1414"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2548"/>
            <end position="2558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2559"/>
            <end position="2568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2569"/>
            <end position="2578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="2770"/>
            <end position="2785"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="4759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="4809" mass="525832" checksum="02135AAA8D69BB4A" modified="2014-04-16" version="1">[encoded data, 4809 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2K5</accession>
    <name>W5N2K5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:ch211-28p3.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2K5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014864"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014893.1">
        <property type="protein sequence ID" value="ENSLOCP00000014864.1"/>
        <property type="gene ID" value="ENSLOCG00000012083.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165556"/>
    <dbReference type="HOGENOM" id="CLU_055828_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2K5"/>
    <dbReference type="OMA" id="CPGHDNQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012083">
        <property type="expression patterns" value="Expressed in larva and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF41">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="157"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="358" mass="41248" checksum="928DC7249D96993E" modified="2014-04-16" version="1">VFFSRSQDVLTKQQEHLKRQREAVKYRIKKLTAKQTEITKKSTAVRENISKKFEDIKRVLDQDLKVTLNQLEMEANAALFSIDNLLEKCNLVTQDIEMELSELNAQLEQNNQQEQHIKTVKKERKVTAIMNISFPDSIQLDDYKSNQILSLINNLFLFIRAQIPITRKVFESYVTEVRLDPDTAHPNLVISPDGTTATYTKEWQEFPESADRFDTTLNVISCDGFSDGRLYWEADVEGKTYWELGLTYPRIPRKGREETCWLGRGEESWCVEFFNGDYTAWHNGVSHHLPISKNFKKIGTYLSYPAGLISFYGIDDQTHLFTFCAGRFNETLHLAVCPGHDNEGSNTKPIKLCSAVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4B7</accession>
    <name>W5N4B7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">NAD-dependent protein deacetylase sirtuin-7</fullName>
            <ecNumber evidence="2">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">NAD-dependent protein deacylase sirtuin-7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Regulatory protein SIR2 homolog 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">SIR2-like protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-decanoyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-decanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:70631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:17932"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:143222"/>
            <dbReference type="ChEBI" id="CHEBI:189688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:70632"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:47665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-propanoyl-L-lysyl-[protein] + NAD(+) + H2O = 3''-O-propanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:23500"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13758"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138019"/>
            <dbReference type="ChEBI" id="CHEBI:145015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:23501"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47669"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with UBTF and the RNA polymerase I complex. Interacts with components of the B-WICH complex, such as MYBBP1A, SMARCA5/SNF2H and BAZ1B/WSTF. Interacts with ELK4, leading to stabilization at target promoters for H3K18Ac deacetylation. Interacts with histone H2A and/or histone H2B. Interacts with DNMT1. Interacts with SIRT1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sirtuin family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01000349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4B7"/>
    <dbReference type="FunCoup" id="W5N4B7">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015476"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015505.1">
        <property type="protein sequence ID" value="ENSLOCP00000015476.1"/>
        <property type="gene ID" value="ENSLOCG00000012572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687441"/>
    <dbReference type="KEGG" id="loc:102687441"/>
    <dbReference type="CTD" id="51547"/>
    <dbReference type="eggNOG" id="KOG1905">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159703"/>
    <dbReference type="HOGENOM" id="CLU_023643_6_2_1"/>
    <dbReference type="InParanoid" id="W5N4B7"/>
    <dbReference type="OMA" id="SNREYCK"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012572">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097372">
        <property type="term" value="F:histone H3K18 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061697">
        <property type="term" value="F:protein-glutaryllysine deglutarylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.200:FF:000002">
        <property type="entry name" value="NAD-dependent deacetylase sirtuin-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000038">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
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    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF1">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867162" description="NAD-dependent protein deacetylase sirtuin-7" evidence="20">
        <location>
            <begin position="17"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="21">
        <location>
            <begin position="115"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050237"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051105"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051399"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052763"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062653"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074008"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="437" mass="48969" checksum="7208F737C88675B5" modified="2014-04-16" version="1">MCVFPLSCVLVVLNVGRLFRYIPTTGRMEGTARDCGRVSSRTERKAQERAKILQQEKQRKTFKLVSQILKKSESERTEEELATLQQCEKMVQELRLRQIRRDALKRKQEEVLDRPDELQRKVKELAAAVRAATYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSASDLSEAEPTLTHMCIGKLHEEKLVQHVVSQNCDGLHLRSGLPRQALSELHGNMYIEVCTACSPPEECVRLFDVTEQTGLHRHGTGRHCSRCGAELRDTIVHFGERGTLDQPLNWKGAAEAAAKADAILCLGSSLKVLKKYACLWCMNRTPNKRPKLYIVNLQWTPKDDLAALKIHGKCDDVMKLLMEELDIQIPEYDKSKDPIFSLATPLRPEEADSHHRKLIAPPSGRPAIFQGSAEECGRAPVQGGWFGRGCSKGRKKKKPLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5U4</accession>
    <name>W5N5U4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01026082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5U4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016003"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016033.1">
        <property type="protein sequence ID" value="ENSLOCP00000016003.1"/>
        <property type="gene ID" value="ENSLOCG00000012993.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164068"/>
    <dbReference type="HOGENOM" id="CLU_000022_46_2_1"/>
    <dbReference type="InParanoid" id="W5N5U4"/>
    <dbReference type="OMA" id="RTDDWIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012993">
        <property type="expression patterns" value="Expressed in liver and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001676">
        <property type="term" value="P:long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05938">
        <property type="entry name" value="hsFATP2a_ACSVL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000005">
        <property type="entry name" value="Solute carrier family 27 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF23">
        <property type="entry name" value="VERY LONG-CHAIN ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="19">
        <location>
            <begin position="60"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="20">
        <location>
            <begin position="498"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="70127" checksum="EECC4F2EC7797B1E" modified="2014-04-16" version="1">CKMHIIFAILAGLAILPLLFRSFFPYIVQDWAYMANVMGSIVKFAKRSRRNPPYLVLDRFFEQVQRHPDKPFIVSRDHTYSFRETDKLSNKFARVLRIHADLKEGNTVALYIGNEPAFIFIWLALAKIGCTVALLNCNIRSKSLLHCFSCCDAKTLIAASELTDAVVEVLPFLREQKASVFILSAESTIPGTETLIDKIDQASDEPIPQSERSSITMQSPAVFIYTSGTTGLPKAAVINQKRLLSALVVLSSVGVHSDDVVYVNLPLYHSAAFLIGFIGSIEAGSTIVLSRKFSVSQFWEDCRKYNVTVMQYIGETMRYLCNTPKKDDDRDHKVRIAIGNGLRADVWREFLTRFGSVHITEFYGATEGNIGFINYVGKIGAVGRVNYLHKKLFRYALIKYDIEKDEPVRDSQGLCLEASKGETGLLVAKITNIAPFSGYAKNHQQTDRKKLRDVFQKGDLYYNSGDLLRIDQDNFIYFQDRVGDTFRWKGENVSTTEVADIFAMVDCIEEANVYGVQVPGHEGKIGMAAVILKQGRQFDCCGMFNHVVSFLPSYARPRFVRVQNSLEVTGTFKQKKVKLVAEGFNPAVLQDPLYFLDDKEKRYVPMTQEIYDSILSMDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N845</accession>
    <name>W5N845_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Signal peptidase complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SPCS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N845"/>
    <dbReference type="FunCoup" id="W5N845">
        <property type="interactions" value="1148"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016804"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016834.1">
        <property type="protein sequence ID" value="ENSLOCP00000016804.1"/>
        <property type="gene ID" value="ENSLOCG00000013631.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009223"/>
    <dbReference type="HOGENOM" id="CLU_068714_1_0_1"/>
    <dbReference type="InParanoid" id="W5N845"/>
    <dbReference type="OMA" id="FWDDGHG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013631">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="180" mass="20167" checksum="3F6AC35B87955F81" modified="2014-04-16" version="1">MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVDISVSRVMLKNVDDFTGPRERSDLGFVTFDISAHLQPIFDWNVKQLFLYLSAEYSTKSNALNQVVLWDKIVLRGESTKLSLRDMKSKYFFFDDGNGLRANKNITLTLSWNVVPNAGILPLVTGSGHKSIPFPDSYETTKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8G4</accession>
    <name>W5N8G4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Storkhead box 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01002143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8G4"/>
    <dbReference type="FunCoup" id="W5N8G4">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016923"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016953.1">
        <property type="protein sequence ID" value="ENSLOCP00000016923.1"/>
        <property type="gene ID" value="ENSLOCG00000013723.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3897">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055589"/>
    <dbReference type="InParanoid" id="W5N8G4"/>
    <dbReference type="OMA" id="KECKDSY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013723">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019391">
        <property type="entry name" value="Storkhead-box_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040126">
        <property type="entry name" value="STOX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437:SF2">
        <property type="entry name" value="STORKHEAD-BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437">
        <property type="entry name" value="WINGED HELIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10264">
        <property type="entry name" value="WHD_Storkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix Storkhead-box1" evidence="2">
        <location>
            <begin position="115"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="801"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="973"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="984" mass="109317" checksum="35E7078088FEB15D" modified="2014-04-16" version="1">MERFLQIAPHSLAIVLSRAGAEDAPAVTDRLQHHHTGYEIFADFKAENMQHFWNKKVTDAISETFFLGWIDEHVLLIQGREDHLEVLREGWMRRSLKPPRGFEINCIRDVSPISMSPISQSQFIPLGEILCLAISAMNSARKPVTQEALMEHLATCFPGVPTPSPEILRHTLNMLVRERKIYPTPEGYFIVTPQTYFITPSLIRTNSKWYHLDERIPDRPQQCTSPQSGTITPSASGCVRERAHHKNHCDSYNCYRDDMHSLHASTLQRKSPKDLKDPYCPPSLYQAAATEKSKSTVNFSYKADTLTKHKEGGSGGEKQPKKFGLKLFRLSFKKDKTKHLATFSAQFPPEEWPLRDEDTPTTIPREVEMEIIRRINPDLTVENVMRHTALMKRLEEEKAQRSKAGSSAQHSARSKRSRGHRKSHGKSRSHSKTRASKGDPSEGSHLDLAGERDYDFYSSSLVRSPRDGTFSAAHNGGNFVLHSNPSVVESHFPVTPEWDVSGELAKRRTEMPFPEPSRGTSHSKVHRSHSHTQDRKSRNERSGKAKERSRSMDNSKGPLGAASVGTAEDFEGCSPDDRGQGRYYVDDGTLRPSQTHHYQRILLSAANFNAEVCVPDVGKGGFDDSKVCSPLEPNRTRDSLSSFSELKPCSPKPTADDYFQCNTSNETVLTAPSPLVKNDHDTLTLSDGVKKVSPGDRQTPLLNRDSCEYKEDATKGQNGISVPAPSPTPEPIPNGRLIHQHNADPCSADKRKEIFSKDTLFKPPHNPLAVGYVENPYQKSGTLRKAQHGKSAEVLGGCEHFEQPGLLSGSPGPSAPQGPDQSSGAEAAFDYYNVSDDDSDEGAHKNAEEEKSREGSGTMQWLLEREKERDLQRKFEKNLTLLSPKESENNNSQKSAHSARLDSMDSSSITVDSGFNSPRTRESLASNTSSIVESNRRQNPALSPGHVGMSSTPSFSFRTAADTPAAEPEKLQKPSNCLASITSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N957</accession>
    <name>W5N957_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vigilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01029663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="W5N957">
        <property type="interactions" value="529"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000017166"/>
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        <property type="protein sequence ID" value="ENSLOCP00000017166.1"/>
        <property type="gene ID" value="ENSLOCG00000013909.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165211"/>
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    <dbReference type="OMA" id="WGPNMKP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in testis and 10 other cell types or tissues"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd22405">
        <property type="entry name" value="KH-I_Vigilin_rpt1"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt10"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt11"/>
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    </dbReference>
    <dbReference type="CDD" id="cd22415">
        <property type="entry name" value="KH-I_Vigilin_rpt12"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd22416">
        <property type="entry name" value="KH-I_Vigilin_rpt13"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt2"/>
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        <property type="entry name" value="KH-I_Vigilin_rpt3"/>
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    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22410">
        <property type="entry name" value="KH-I_Vigilin_rpt7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="High density lipoprotein binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000061">
        <property type="entry name" value="High density lipoprotein binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000018">
        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vigilin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
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        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
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        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF67">
        <property type="entry name" value="HIGH DENSITY LIPOPROTEIN-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="147"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="219"/>
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        </location>
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    <feature type="domain" description="K Homology" evidence="9">
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    <feature type="domain" description="K Homology" evidence="9">
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    <feature type="domain" description="K Homology" evidence="9">
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            <begin position="432"/>
            <end position="500"/>
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    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="504"/>
            <end position="573"/>
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    </feature>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="621"/>
            <end position="690"/>
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    </feature>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="695"/>
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    <feature type="domain" description="K Homology" evidence="9">
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    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="841"/>
            <end position="932"/>
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    <feature type="domain" description="K Homology" evidence="9">
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            <begin position="933"/>
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    <feature type="domain" description="K Homology" evidence="9">
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        </location>
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        <location>
            <begin position="1084"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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    <sequence length="1229" mass="137616" checksum="646520EA8415E691" modified="2014-04-16" version="1">MSSVAVLTPESFAEHRSGLRDEEIAAGGAPEDEAYIPTYLEAFPPLPEKGAPGEKSNEPSGAWSKIRPIKSSVITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQKTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVTIPKEHHRFVIGKNGEKLQELELKTATKINIPRPDDPSNQIKITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAYNKTVQEISMETGARINIPPPSVNKDEIIITGEKEPVAQAIARIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILETTGVSVEMPPLDSGSETIILRGEPDKLGPALTQVYAKAKSVMVAEVTAPAWLHRFIIGKKGQNIGRITQQLPKVHIEFTDGDEKITLEGPTEEVEQAQTQIQDIIKDLLVRMDYTEVTIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSGLVRIEGDPQGVQLARRELMEMAARMENERTKDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVEKCSKYLQKVIIELVRLVNNKKIDLPTENSNSEVIVITGRKASCEAARDRILAIQRELANIKEAEVSIPSKLHNSLIGAKGRLVRSVMEDCGGVHIHFPSEGSGSDKVTIRGPSDEVEKAKRQLLQLAEEKQINNFTVELQAKPEYHKFLIGRGGGNIRKVRDRTGARIIFPSADDHEQELITIVGREEAVRQAQRELETLIKNLDNVVEDTMVADPRYHRHFVARRGQVLREIAEEYGGVTVSFPRTGVQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVTVECVIPQKYHRAVMGPKGSRVQQITRDHDVQIKFPEREESLGVEQHPQENGDVSAELDLLPKKSDIIVISGRKEKCEAASVALLALVPVTVDVEVPYDLHRYIIGQKGSGIRKMMEGYEVNISVPQPEQQSDTIKITGQVANVERARLGLLERVKELQAEQEDRALRSFKLTISVHPKYHPKIIGRKGAVISQIRKDHDVNIQFPDKGDEQQDLITITGYERNAEAAKQAIERLVANLEEMVSEDIKLDHRVHARIIGGRGKAIRKLMEEFKVDIRFPQPGSEDPDRVTVTGLPENVDNAIDHLLNLEEEYMMNVTETETMAAYMKPPSRSEDDHRGPAKGFVVRDAPWNVPGNKAPDMSSAEDFPTFGTGVAPKQMSAWGPKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9P6</accession>
    <name>W5N9P6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein polypeptide A'</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing as component of the spliceosome. Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in the spliceosome B complex. Identified in the spliceosome C complex. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B. Contributes to the binding of stem loop IV of U2 snRNA with SNRPB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628826.2">
        <property type="nucleotide sequence ID" value="XM_006628763.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9P6"/>
    <dbReference type="FunCoup" id="W5N9P6">
        <property type="interactions" value="1922"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017355"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017386.1">
        <property type="protein sequence ID" value="ENSLOCP00000017355.1"/>
        <property type="gene ID" value="ENSLOCG00000014084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696562"/>
    <dbReference type="KEGG" id="loc:102696562"/>
    <dbReference type="CTD" id="6627"/>
    <dbReference type="eggNOG" id="KOG1644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153289"/>
    <dbReference type="HOGENOM" id="CLU_061027_0_1_1"/>
    <dbReference type="InParanoid" id="W5N9P6"/>
    <dbReference type="OMA" id="QPREFPQ"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014084">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="11">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="222"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="261" mass="29269" checksum="EBCE045318681682" modified="2014-04-16" version="1">MVKLSAELIEQAAQYTNPVRDRELDLRGYKIPVLENLGATLDQFDTIDFSDNEIRKLDGFPLLKRIKTLLMNNNRICRIGEGLEQSLPNLRELVLTSNNIQELGDLDPLASVKSLTLLSLLRNPVTNKKHYRLYVINKIPQIRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIAKRTKTFTPGAALQAEKKKTGPSPADVEAIKNAIANATSLAEVERLNGLLQSGQIPGRDKKQGPAGEEEEQEEEEEQMDEDIPVNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9U2</accession>
    <name>W5N9U2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="3">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:24381"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01024789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9U2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017401"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017432.1">
        <property type="protein sequence ID" value="ENSLOCP00000017401.1"/>
        <property type="gene ID" value="ENSLOCG00000014123.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017828"/>
    <dbReference type="HOGENOM" id="CLU_033347_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9U2"/>
    <dbReference type="OMA" id="GASPIHE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014123">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071951">
        <property type="term" value="P:conversion of methionyl-tRNA to N-formyl-methionyl-tRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12230:FF:000003">
        <property type="entry name" value="methionyl-tRNA formyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="12">
        <location>
            <begin position="83"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="13">
        <location>
            <begin position="210"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="41013" checksum="B69030184AFA90E6" modified="2014-04-16" version="1">GSSAQDLSQSKARARPPWRIVFFGTDDFALESLKILTESRQKHSERLIDTLEVVTLPKELPVRRFASQNDLPIHDWPDMNLREQFDVGVVVSFGCLLRKNLIEQFPYGILNVHPSLLPRWRGPAPVFHTILNGDTVTGVTIMQIRPKRFDVGPVLSQELYPVPPNCTAEQLGASLATLGAQMLICTLKNLPERIANRREQSKDGATLAPKISTAMSWINWEEQTCDQINCLFRAISFRIPLRTLWMGETIKLLDFVGKFKTPVSEMEGAQAPAPGSIQFKKETNTLLVRCKDGWVGFKAVKLKKRLSAADFYNGYLHQYFLKKSSTPHIECLFQNPRPQPRDSHSGKEVQSEMEGLTRGRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB31</accession>
    <name>W5NB31_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkey-109j17.5</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006656">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB31"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017872.1">
        <property type="protein sequence ID" value="ENSLOCP00000017840.1"/>
        <property type="gene ID" value="ENSLOCG00000014494.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691382"/>
    <dbReference type="KEGG" id="loc:102691382"/>
    <dbReference type="eggNOG" id="KOG1121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161131"/>
    <dbReference type="HOGENOM" id="CLU_405412_0_0_1"/>
    <dbReference type="InParanoid" id="W5NB31"/>
    <dbReference type="OMA" id="LISHMKR"/>
    <dbReference type="OrthoDB" id="10057873at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014494">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="559"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="637" mass="70276" checksum="573E17A3D14ADBA5" modified="2014-04-16" version="1">MASHSRVSILDYFNIVFEGENGKIESNCKACGTRIQAKRTVTSNFVTHLKRKHQAMYDEFVKKKDIKREAYSSGGVHLAPSGRAEPPARPSHSHSHSHTHSLSLSLGTGGGGGGGVAKFERGDPRQAIISEAIAKMIIRDLQPVRIVESEGFRELLRLLEPRYTPESRKYVQQQLLPGYAYQAQLASKQALQAAESCSVTLDVWRSGPASGYLGVTCHFITSDWQIKSALLACLRLSGPRTAERILPEYDEISRAHGIAGKVFRVVADGGSDSRASFRLPGFCLQSGDEEEEEDEDEDEEGSGDEEGGGEGLCLGPRRVACFARSLARCVRDGLRASPPLALALTKAACFLNYVRATVAPEKLQPALGRAPAGERRWNAQLKAVRRLLEAADFLEDVAGRRDLALGAFEKAALRELVELLEPFEEATDMVEGDKHVPVSLALPCVLGLRKHLAESAPRQCAGLLGSLAQALDRRLARLLEDPLHVTATALDPQFKLSWSSSREQHRQVVLEEVSQHGPSPPPSQAPRPPPCKRSKLFSFMRPRPAAPARSLEQELAAYLHEDPTDEDPLHYWRRKAADFPQLAHVAKRVFTVPATSTPVERIFGTVGRIARPERCQLLPRNLETLIYLKANYRLLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBA9</accession>
    <name>W5NBA9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the THO subcomplex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7. The THO subcomplex interacts with DDX39B to form the THO-DDX39B complex which multimerizes into a 28-subunit tetrameric assembly. Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; in the complex interacts with THOC1, THOC3, THOC5, THOC7 and DDX39B. TREX seems to have a dynamic structure involving ATP-dependent remodeling. Interacts with POLDIP3 and ZC3H11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NBA9">
        <property type="interactions" value="1735"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017918"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017950.1">
        <property type="protein sequence ID" value="ENSLOCP00000017918.1"/>
        <property type="gene ID" value="ENSLOCG00000014546.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00710000106792"/>
    <dbReference type="HOGENOM" id="CLU_000511_5_0_1"/>
    <dbReference type="InParanoid" id="W5NBA9"/>
    <dbReference type="OMA" id="QERWTCI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014546">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="10">
        <location>
            <begin position="421"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="9">
        <location>
            <begin position="569"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="8">
        <location>
            <begin position="874"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1189"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="897"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1205"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1221"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1245"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1268"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1299"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1412"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1424"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1446"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1518"/>
            <end position="1588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1588" mass="181657" checksum="0C7F3F403EBAEAF5" modified="2014-04-16" version="1">KMAAVVLPTDWIKNWEKTGKNEFVQLCRNLADNKTNDDSTCLISLDIHAALYELSWHVIRGNLKLDLAASVLGDIMELREDMSSILADVFCILDIETGCLEEKNKRDHFTQLVGACLYFVSDAVLKERLDPETLESLGLIKQAQQFNQKIVKIKTKLFYKQQKFNLLREENEGYAKLITELGQDLSGNITSHLILENIKSLIGCFNLDPNRVLDIILEVYECRSEQDEFFIPLIKCYMCEPQTLCHILGFKFQFHQEPNGETPSSLYHVAAALLQHNLIELEDLYVHLLPLDPTILEEHKREIMEAKQIARKLTMVVVPSEKTDEKDKEKEKEEEKNEKPPDNQKLGLLEALLRIGDWQHAQSIMDQMPSFYATSHKPIAVALCQLLHFTIEPLYRRAGVPKGAKGCLMRPLTNKRAPKPAETFEELRKEVFSMLCYLGPHLSHDPILFAKIVRLGKGFMKEYQNESRQDEKDKMDTLLSCFLSIADQVLLPSLTLMDCNACMSEELWGLFKMFPYQHSYRYRLYGQWKNETYNSHPLLVKVKAQTVDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQWYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKERMKHDDTTISAWLQSLASLCGAVFRKYPIELAGLLQYVTNQLKAGKSFDLLILKEVVQKMAGIEITDEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHELALPLCLLMAQQRNGVVFLEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSVDVLCNQFHTPHDAAFFLSRPMYAHQILSKYDELKKAEKGNRQQQKVHKYITACDLVMAPVHEAVVSLHPPKVWDDIRPQFYATFWSLTMYDLAVPHNAYDREVNKLKVQMKAIDENPEMPPNKKKKERERCTALQEKLQEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFCTLLCYDRVFSDIIYTVASCTENEARRYGRFLCCMLETVTRWHSDRAIYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILQCYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKPYMVPENEFHHKEQPVRSATPANLQNGPGSAGKPAATTATTGKPEEGVTEETDKSKEKSQGPVKAANKTNSATTKVTTSNGNSAPNSTKAIKEKEDKEKSGKEKSKDKKEKTPAGTPEAKVTGKEKQKEERLGKDDKARETKEKTPKADKEKEKLKKDEKPGKDDKVKLSSNTEFRSSADKEPSKERDIAKEAKVKESSKGEKVAATGSLKSPVPRSESSESERELKRRKLDTHSSPSHSSTVKDNLNELKDSASKHYANHSTATQSKSKEREMDKKEMEKSRDRSKEREKKEEKDRKERKRDHSNSDRELSQELKRRKDENGTGSSKHSKSASPSDSPRSSEKEKDKSKSSKPSSKEKSDLIKPEKSSSGGKKESRHDKEKSEKKEKRDSTGAKEDKNIDTHKSSDKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBB8</accession>
    <name>W5NBB8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBB8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017927"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017959.1">
        <property type="protein sequence ID" value="ENSLOCP00000017927.1"/>
        <property type="gene ID" value="ENSLOCG00000014569.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005780"/>
    <dbReference type="HOGENOM" id="CLU_1337129_0_0_1"/>
    <dbReference type="InParanoid" id="W5NBB8"/>
    <dbReference type="OMA" id="GWINCAF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014569">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF11">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="205" mass="23197" checksum="2E857128000F2A9F" modified="2014-04-16" version="1">MAGPELLLDSSIRVWVVLPIVFVTFFVGVIRHYVTQLLHSDKKVDLQQVSDRGLGHEPSCVQPEGLACLLPTDPPVFSLRTAKVPFPLTLRFKPMLQRGIELLSLDASWVSSASWYFLNVFGLRSMYNLILGHDNAADQSRIMQDHMTGAAMAMPPDPNKAFKSEWEALEIVEHQWALETVEEELMSRDLNFGGFFSREVKTTMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBT8</accession>
    <name>W5NBT8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069043875.1">
        <property type="nucleotide sequence ID" value="XM_069187774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBT8"/>
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        <property type="interactions" value="1380"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018097"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018129.1">
        <property type="protein sequence ID" value="ENSLOCP00000018097.1"/>
        <property type="gene ID" value="ENSLOCG00000014699.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685178"/>
    <dbReference type="KEGG" id="loc:102685178"/>
    <dbReference type="CTD" id="92609"/>
    <dbReference type="eggNOG" id="KOG2832">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240503"/>
    <dbReference type="HOGENOM" id="CLU_048293_1_1_1"/>
    <dbReference type="InParanoid" id="W5NBT8"/>
    <dbReference type="OMA" id="WKSLHYY"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014699">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="14">
        <location>
            <begin position="183"/>
            <end position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="360"/>
            <end position="393"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="393" mass="43892" checksum="4573156AAEADDD1F" modified="2014-04-16" version="1">MSAGYVYNVCVRTSRGLLRLGPGIRAGPGAAVPAGIRAMHSDRPAGREDSGTGGLAQAILQDRLQQQQLKSQGEPAGDAEGQGQQGQQDRGDERKQKENTAYAKKIVLRLAGVLALGGSVGVVYLFGSSSMDEQGNKIPDEFDGDPPVIQQVRRTYKYFKDYRQMIIEPTSPKLLPDPLKEPYYQPPYTLVLELTDVLLHPEWSLVTGWRFKKRPGIDFLFQQLAPLYEIVIFTSETGMTAFPLIDSIDPQGFVMYRLFRDATRYVEGHHVKDVSCLNRDTSKVIVVDCKREAFSLQPFNGLALQKWDGNSEDRALYDLAAFLKTIALSGVEDVRTVLENYAHEEDPIAAFKRRQAQLAQEEQQRLSESSQHKSQGLSLGSIAGRFWPRSKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC12</accession>
    <name>W5NC12_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dispatched RND transporter family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dispatched family.</text>
    </comment>
    <dbReference type="FunCoup" id="W5NC12">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018171"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018203.1">
        <property type="protein sequence ID" value="ENSLOCP00000018171.1"/>
        <property type="gene ID" value="ENSLOCG00000014758.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696811"/>
    <dbReference type="KEGG" id="loc:102696811"/>
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    <dbReference type="eggNOG" id="KOG3664">
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    <dbReference type="OMA" id="MPVTIVW"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035195">
        <property type="term" value="P:miRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Dispatched RND transporter family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052081">
        <property type="entry name" value="Dispatched_Hh_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951:SF2">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951">
        <property type="entry name" value="PROTEIN DISPATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="567"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1035"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1064"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1108"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="535"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1216"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1289"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1394"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1469"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1294"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1436"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1469"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1498"/>
            <end position="1509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1509" mass="165749" checksum="A6BD229E3253A05A" modified="2014-04-16" version="1">MDACSVSEENAHVIIMDSSPAEGPEQETSLSEIPEVALCPPESPDREPRLTLIHTDGDRLSSPGHPRTASLSQLYHQVSQGCSHQSPLLKRCRYCGHRQPVTSSLCPERHSGPASPRPDCSSATVKTADSCSPSHVPSCHDTIHCHWLQGSHDAGNHQAMQHHVVTVRHERLYRIPRSYSQLVADCPVALLITCAVVFLGCSLAGILTGPLPDFSDPLSGFEPRGTEIGRRLSAWNRLQENTGPEKTLSLSPRQLTEQRVISYDEEGGGGTGKSRDWPSPRQRRMLDWDFTQDTFFCDSPGEKYAQLVFRSGNSASLWSLQAIHSMCEMEQARIRSHALFQDLCKHRGGEDGEGDTGMRECCPSWSLGNYLAVLSNATSCLGLSAAQVSESLQLLRQCAPYYHDGSLAPACAEKGKLGQCSTVPSQCKHSSAIYQILHYLVDKDFLGPQTIGYKVPSLKYSLLFLPVEKGDHMMDMYLDNLEGRDLTNDNTTITGMDLGIKKQLFQYYLARDSVYPVLAVVMLFLTMAFYLHSLFLSAMSLAAILGSLMSSYFFYKVAFRLTFFPFLNLAALLVLLGSCANQAFIFADLWSLQLSQKPPASLEKRVKRALQEAGYLILVSGLTSSAAFYSGYMSSITAVRCFAVYLGTATLISSLSSLVWLPCILILHERYAVAAAAVEAPAGKTCCALSPGGFWDTSSRKRCLFSLGQKLRGLRRGLTDTSDLLFIKILPCGVVKFRYIWVCWFAVLAAGGTYITCVDPGMKLPTLESKAAQLFRSSHPFERYDAEYSHQFMFERLKSGEEKPMTITLVWGVLPTDNGDHLDPKSNGSLVLDPEFNMSSPDAQSWLRDLCPRIQNQSFYLPISSSSEEEKQGDSVCFVERLLRWVSIRQCSESEGAYSLCCNDILFPYLPGVFEHCLGMMAAEKEAEGFLFDTGGPRFDSEGRITALILEFTTTHLHSYNYSQSAHFYQQIEAWFHREISGAPPGLRNGWFVSQLALYDLQQCLSSETLVVTGFSVALVFAMLLLTTWNIPLSIYGSAAVGGSVFVTAGLLVLLEWQLSGLEALFISAAAGLSVDFAANYCVSYSLSPHPDRLGRVAHSLKRMGCPAAIGAGAFFCMGIIMLPATALLFRKLGIFLLLVKCVACGFATFFFQSLCCFFGPQKNCGQIFWPCTTASGHYKADSESGNLLSTAAAGSSNSAANGAFGCGGRSRVRRSFGKAEGGGQLCPSQQRQRQRQGGGGGRESEQYELQPLACQLSDSFENSTCTSKLSNRPSVLSEEIQFRSLSPRKDLEEFNTEDDSEELTSRHLKTFPPPPALQTSSPYKENTLRPVVVPPSEMVQERLLCRRCRGQAGGIKHWNVSLSSSSSMEDIIISSQPPDMDHRHSLSIEGAMSSPFKRHPHRRLLSSQSQSSFEGLEDSNETCLSDVEPGPATPQPTTEAQAESQPGYLNGKRDTLRLSLKETVFEASSQGIGRGRTSQGELPVILPNSKPDLPDVWIKREGRSGDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND59</accession>
    <name>W5ND59_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hypoxanthine-guanine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:25426"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:17975"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01016966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND59"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018568"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018600.1">
        <property type="protein sequence ID" value="ENSLOCP00000018568.1"/>
        <property type="gene ID" value="ENSLOCG00000015083.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155028"/>
    <dbReference type="HOGENOM" id="CLU_073615_3_0_1"/>
    <dbReference type="InParanoid" id="W5ND59"/>
    <dbReference type="OMA" id="YQAYEIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015083">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000019">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF6">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869558" description="Hypoxanthine-guanine phosphoribosyltransferase" evidence="18">
        <location>
            <begin position="17"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="19">
        <location>
            <begin position="145"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="34838" checksum="A582B84787C74FD5" modified="2014-04-16" version="1">MLLFFFFLPLNSPARAHPHCAFARAPLAEGSAAGHARRGHARSRGVGGACRLSLFLRSATPRQRRQAGGPGVLSSCGTFAGAPGTRRGAARLGLSGGMAAAESCVVISDEEQGYALDLFCIPKHYAGDLERVYIPHGLIMDSRTERLAREIMRDMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGEIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMKTLLELLKQYNPKMVKVVSLLVKRTPRSVGYRPDYIGFEVPDKFVVGYALDYNEYFRDLNHICVISEDGKEKYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDA4</accession>
    <name>W5NDA4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWAP70 N-terminal EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDA4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018613"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018645.1">
        <property type="protein sequence ID" value="ENSLOCP00000018613.1"/>
        <property type="gene ID" value="ENSLOCG00000015122.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVV8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183017"/>
    <dbReference type="InParanoid" id="W5NDA4"/>
    <dbReference type="OMA" id="IPAFINT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015122">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057836">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383:SF2">
        <property type="entry name" value="DIFFERENTIALLY EXPRESSED IN FDCP 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383">
        <property type="entry name" value="SWAP-70 RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25530">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWAP70 N-terminal EF-hand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="608" mass="69533" checksum="302624D20C37C9C6" modified="2014-04-16" version="1">MNFKAELLKSIWYAFTSLDVEQSGKVSKSQLKVLSHNLYTALNIPHDPVALEEHFRDDDAGPVSNQGYMPYLNNYILAKAREGTFNKEAFDDLCWMMTSKKNYDTNKLGILSSRDCFRLFCLFNLLSEDTYPLVMIPDEARQTVSLVTSRHKRSWSKLFLNFHISLTPDDQKEAQCIPAFINTASFPKLLAAESKAPASSASRQVVPEYLKQPSCWSSEAYRANIKTNPGILKQALSLLSPGDGLHWRAETKQKRLSCVGGEVETDSGPDASTATKEAAAGFQCDASSRPPAIQTAVRLQAEGKTSLHRELKLRRREQRQQSPHAREEPPGLQVLQEERGRQELEQEQLVRRQQEQERRLQEEQERKRQLEVQRSLERQLEEAERVRESMAAEIARKEQEARGQRRRIEELELTQQSLERALNAEIQARLEEETARLEQERLLSQEEEKLQHLSLLKQEQDLLLQQAQLERSMLEAEVADKVQAFETASQELQRLRESRERSNHDLQVAQKKLRRASRHVKHWNVQLNRLMQPIAPGGVQDGELISESTLTETRGALSSKEFIARRCPGNMPVSECSSSSEDEGLEEDLQKISLSGANRRRMEPNGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDE1</accession>
    <name>W5NDE1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zic family member 3 heterotaxy 1 (odd-paired homolog, Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632948.1">
        <property type="nucleotide sequence ID" value="XM_006632885.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDE1"/>
    <dbReference type="FunCoup" id="W5NDE1">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018650"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018682.1">
        <property type="protein sequence ID" value="ENSLOCP00000018650.1"/>
        <property type="gene ID" value="ENSLOCG00000015155.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693820"/>
    <dbReference type="KEGG" id="loc:102693820"/>
    <dbReference type="CTD" id="7547"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160788"/>
    <dbReference type="HOGENOM" id="CLU_002678_37_1_1"/>
    <dbReference type="InParanoid" id="W5NDE1"/>
    <dbReference type="OMA" id="GTPRHHD"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015155">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000039">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="384"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="448" mass="48852" checksum="4B27C86B3B3B7315" modified="2014-04-16" version="1">MTMLLDGGPQFSALGVGGFGTPRHHEMGNRDPGLGLNPFGESSHSAAFKLSPVAHDISSSQTSAFTPQASGYATALGHHHGGQVGSYGGGAFNSARDFLFRNRGAGIGDSAAAGGQHGIFAPSAGSLHGPPGISDNPGHLLFPGLHEQTASHPSPNGHVVNGQMHLGLRGDLFGRPDPYRPVASPRTDPYATAQLHNYNHPINMNMGMNVPTHHGPGAFFRYMRQPIKQEMSCKWIDETQMNRPKKTCDRTFSTMHELVTHVSMEHVGGPEQSNHICFWEDCPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKVHESQASESSPAASSGYESSTPPALVSTSTEDPTKTPPSAVQNTPTHSEGLPPNFNEWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDG2</accession>
    <name>W5NDG2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin, light chain 7, regulatory</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625575.1">
        <property type="nucleotide sequence ID" value="XM_006625512.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDG2"/>
    <dbReference type="FunCoup" id="W5NDG2">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018671"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018703.1">
        <property type="protein sequence ID" value="ENSLOCP00000018671.1"/>
        <property type="gene ID" value="ENSLOCG00000015174.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697662"/>
    <dbReference type="KEGG" id="loc:102697662"/>
    <dbReference type="CTD" id="58498"/>
    <dbReference type="eggNOG" id="KOG0031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161682"/>
    <dbReference type="HOGENOM" id="CLU_061288_9_3_1"/>
    <dbReference type="InParanoid" id="W5NDG2"/>
    <dbReference type="OMA" id="ALTHWGQ"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015174">
        <property type="expression patterns" value="Expressed in heart and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055004">
        <property type="term" value="P:atrial cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060038">
        <property type="term" value="P:cardiac muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045823">
        <property type="term" value="P:positive regulation of heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055005">
        <property type="term" value="P:ventricular cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000007">
        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="22112" checksum="44D1005FE0969EC7" modified="2014-04-16" version="1">MSYNPAQRSARLGPLTYPHRADMASRKAATKSKAAAKRSQRGSSNVFSMFEQSQIQEFKEAFSCIDQDRDGIIKKEDLKETYSQLGKLNVSDEELEAMLQEGKGPINFTVFLTLFGEKLNGTDPEDTILAAFKLFDPSATGFVNKDEFRRLLLTQADKFTEEEVEQAFSLAPIDVSGNIDYKSLCYIITHGDEKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE96</accession>
    <name>W5NE96_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Catsper channel auxiliary subunit epsilon</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell projection</location>
            <location evidence="12">Cilium</location>
            <location evidence="12">Flagellum membrane</location>
            <topology evidence="12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CATSPERD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027838">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE96"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018955"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018987.1">
        <property type="protein sequence ID" value="ENSLOCP00000018955.1"/>
        <property type="gene ID" value="ENSLOCG00000015402.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R8SD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162691"/>
    <dbReference type="HOGENOM" id="CLU_014273_0_0_1"/>
    <dbReference type="InParanoid" id="W5NE96"/>
    <dbReference type="OMA" id="YRHCFSY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015402">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097228">
        <property type="term" value="C:sperm principal piece"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028751">
        <property type="entry name" value="CATSPERD/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053814">
        <property type="entry name" value="CATSPERD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053816">
        <property type="entry name" value="CATSPERE_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053815">
        <property type="entry name" value="CATSPERE_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053818">
        <property type="entry name" value="CATSPERE_NTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053817">
        <property type="entry name" value="CATSPERE_NTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33722:SF3">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED AUXILIARY SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33722">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN SUBUNIT DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22844">
        <property type="entry name" value="Beta-prop_CATSPERE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22850">
        <property type="entry name" value="CATSPERD-E_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22849">
        <property type="entry name" value="CATSPERE_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22841">
        <property type="entry name" value="CATSPERE_NTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22843">
        <property type="entry name" value="CATSPERE_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="886"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERE first N-terminal" evidence="14">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERE second N-terminal" evidence="15">
        <location>
            <begin position="77"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERE beta-propeller" evidence="16">
        <location>
            <begin position="206"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERE Ig-like" evidence="17">
        <location>
            <begin position="566"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERD/E C-terminal" evidence="18">
        <location>
            <begin position="714"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="934" mass="105475" checksum="AA26CE50545A7115" modified="2014-04-16" version="1">SYNEPIYNIFLFESRILLEYEGSSFTEWSYPEFCEVDNKNSPKAVLTCRIAGYHKVKPLVSENEEERHLHIGDSFFCFSWYVAKAPDSRQGMQILRVWVMDPENADPAEINQTALSPSVQSSFISKQFFTLGQDPIIKWSPTKSVQGDFNFVKGYWEIHLTDNEVQSLPNFWIEGKAVGYQNCFIASGYHTFHDVEPLLPPGGREVPLRLPRGSQLSVAWESCSLSTALLLSDFGTFITHDAFGTLAEILVAPHLLAAPAEGHFTLTDAVIVEGSILFLMDGSVYWRSYDRLLRKREEDLPSSGVRGLQYRPTCWQSYPLRDIELSIIAVWTEDQLYLGKSTFYKKADRALLAQILRLPSSCNVTIPAVAFGSRPPEVGVLVEACGAHDSSDARLFLVLYDEEAGSWFQSAFLAKLKPGSLSQGPFRLRFVQSALPSVLLWNKEALYYSSRNNSEYGLLRTPRSANLTAESGGSSIHQVMLDLSWNVVVKMENNALYFCKVGMDELVRLHMWERPGTNMALYLGPKGQMKVLRQVESAIEVHRYPLSMEVKSAVLGTAHTCTYFTFQHSMDIPAYYLDMGEQMELWAQIVYPENMGVNIQISGHRLDLLHVATRTRFEIASQVCTKNTTIKLYHWRDYSKSENYREEVSQTSGIVTLEVVPNLPGNTCNLPSNRISHIKVGCPPKRHIRISRPWGTLCEKLDLKNYSIPGSVLSNPQDQDLVVDYDWEYLGCLFRIYYATPFLPGIDLYDGDTFLKSVEANFIVWEENGRTDYSFNASMETVGCLREAQTWSSMLAGNGSFSSSTPGEAWGPHNYRSCFEVTPGVLGELSQPYEILNRSGNNFITWSQVQSGIYVFSVKILDPNFSFCDLQAVFAVQTYGVVTSNFGVFTGTTAMMVTLIVTGVLICTYFRYIKVFREMLNNPVVDKQSKSKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEI3</accession>
    <name>W5NEI3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanylate cyclase activator 1Aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEI3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019074.1">
        <property type="protein sequence ID" value="ENSLOCP00000019042.1"/>
        <property type="gene ID" value="ENSLOCG00000015471.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164333"/>
    <dbReference type="HOGENOM" id="CLU_072366_4_1_1"/>
    <dbReference type="InParanoid" id="W5NEI3"/>
    <dbReference type="OMA" id="PEANEYI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015471">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000052">
        <property type="entry name" value="Guanylate cyclase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF164">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21964" checksum="7D08997542E8BDDF" modified="2014-04-16" version="1">GTMGNASGSTVDDMQAVEIHHWYKKFMTECPSGQLTLHEFKQFFGLRGLDPEANAYIEQMFRTFDMNKDGYIDFMEYVAALSLVLRGKMEQKLRWYFKLYDADGNGCIDRYELLNIIKAIQAINGCNLEMSVEEFTNRVFEKIDVNGDGELSLDEFVEGARKDEEFMEVMMKSLDLTHIVAMIHSRRHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEM5</accession>
    <name>W5NEM5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01029119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEM5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019084"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019116.1">
        <property type="protein sequence ID" value="ENSLOCP00000019084.1"/>
        <property type="gene ID" value="ENSLOCG00000015507.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160150"/>
    <dbReference type="HOGENOM" id="CLU_001666_3_1_1"/>
    <dbReference type="InParanoid" id="W5NEM5"/>
    <dbReference type="OMA" id="VDDCWRH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015507">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18078">
        <property type="entry name" value="DEXXQc_Mov10L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000999">
        <property type="entry name" value="Mov10 like RISC complex RNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049080">
        <property type="entry name" value="MOV-10-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418">
        <property type="entry name" value="CANCER/TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418:SF1">
        <property type="entry name" value="CANCER_TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21634">
        <property type="entry name" value="MOV-10_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase MOV-10-like beta-barrel" evidence="13">
        <location>
            <begin position="605"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="11">
        <location>
            <begin position="778"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="11">
        <location>
            <begin position="890"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="12">
        <location>
            <begin position="1045"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="716"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="726"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1220" mass="133198" checksum="C0124083A5101869" modified="2014-04-16" version="1">MVTVARSLVAKLLAPLWRKAEEEEGEEGLITSGVEEIRHLHSGVVTRLCQDYGLIDELVCFTAAEVLGGLSVGAGDQVRAVAVRDRAAGGWRALQVERVTDSWEEAGGKTVDSETSPLKSLIGMVTSCEREGGFINHTTYFPQQALCDVCPGFKPVKGDWVQAQYFVSQTQWASQAKSVAPLRYHRIDQVSVSSVHGRTGVVDDKVFFTLDSLLLPDQYTPGAGDLVTVVVVESSQSMYCWRALCMAPSDRSACARSPVQPAPEWENQSLLLNKGDLEVGTDTDFGRLLLGQGRALQVWIENKGTETRHLQGCEFAGRDPEGQFTLCFPREETVPQEMETPSPPSGPVHMGHLPQRQGFLGGYGPLRRPYLGSVLRPPCSISGGGQCPYPVLRLPGPPGGGEGAAREEVEGGGGPPALDQGPGGRVCEKLSAGPDEAEPAGTTRSEIAIPPGQRVCVSVRCQARSLGRCTEILLLHFPGFTIKRRLQARVGSVEESLLQPASPYCPAQPALRARGAETLTVLPGPAPSRTLRRHLPSFLGHYPVPQRLRDCVWASGDVLVAHPELGQPLSVETLQPRFSVLLWLEELKAERELQEFGLGGAILRKSGDHLQLEVPGVAEGRPSVTVGDKVLLKKLLSGGVQVEYIAYVTEISEEELTLRVNSDFQRSYMGEPLDVEFTFNRLTMRRCHCALEQSRHLGETVLFPSSVVLQPPQWNEDWEEEVGSREATQGDQGTSQSDGRDVRMLLMDMVSVATQTKTDSRPQSRPVPSPGCFFNPALNPQQREAVKRILVGECRPMPYILFGPPGTGKTITLIEAILQVHHRLPSSRVLVCTPSNSAADLLCTRLHYSGFLQGGSLVRLNATCRLEESVAEVLRSYCRAGEDVRQAAFHRVVISTCSSAGMLYQIGLRVGHFTHVFIDEAGQASEPEILIPLGLLSERDGQIVLAGDPCQLGPVVKSRLASAFGLGVSLLERLMERPLYLRDEQRYGKGAAYNPLLVGQASGGSHFRSWLSVMALIAQPPFYNKSETSLGMLYSNRLNRDRWCSSPCFLSASQGTEMREGSNPSWFNPAEAVQAMLYCCQLAKRLYRPIAPSDIGIITPYKKQVEKIRVLLQRVGLMEIKVGSVEEFQGQEFLVIVLSTVRSNEDQLGEDGQGVLGFLANPKRFNVAITRPKALLIIVGNPHVLNKDPCFSALLQYAYDNKAFVGCEAPADLGTQSSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF34</accession>
    <name>W5NF34_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transmembrane protein 63B</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="62"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="158"/>
            <end position="178"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="711"/>
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            <begin position="120"/>
            <end position="224"/>
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        <location>
            <begin position="240"/>
            <end position="422"/>
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    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="10">
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            <begin position="433"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="779"/>
            <end position="828"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004127"/>
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            <dbReference type="ARBA" id="ARBA00007779"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="828" mass="94121" checksum="F2A66BA24A2ADBDD" modified="2014-04-16" version="1">MLPVLIATMGGLAEASNTSRDYCYSARIRSTVLQGLPFGGVPTVLALDFMCFLVLLFVFSILRKVAWDYGRLALVTDADRQRKRFSGLEEQEYVASALHSETQDRYERLTSVSSSVDFDQRDNGFCSWLTAIFRIKDEEIREKCGGDAVHYLSFQRHIIGLLVVVGVLSVGIVLPVNFSGDLLENNAYSFGRTTIANLNSGNNLLWLHTSFAFLYLLLTVYSMRRHTSKMHYKEDDLVKRTLFITGISKYAEENQIKMHFEHAYDNCKVLEARLCYNVAKLMSLDAERKKAERSKKFFTDLMEKEHMCTMINPKPCGHLCCCVIKGCEEEEAVSYYTKLAAKLKEEYRTEKEKVNTKPLGMAFVTFQNEAMTAIILKDFNACQCQGCKCRREPRSSTHSESLHVHNWNVTYAPDPQNVYWEHLSLGGLSWWLRCLVINCILFLLLFFLTTPAIIITTMDKFNVTKPVEYLNNPIITQFFPTLLLWAFSALLPTIVYYSAFFEAHWTRSGENRTTMHKCYTFLIFMVLLLPSLGLSSLDLFFRWLFDKKFLADAAVRFECVFLPDNGAFFVNYVIASAFIGNAMDLLRIPGLLMYMIRLCLARSVAERRNVKRHQAYEFQFGAAYAWMMCVFTVVMTYSITCPIIVPFGLMYMLLKHLVDRYNLYYAYLPAKLDKKIHSGAVNQVVAAPILCLSWLLFFSTVRTGFLASTSMFTFVVLIITIVICLSHVCFGHFKYLSAHNYKIDTQETGVDGVENGRPPRFSPSNKSATLYIAQVLQDPNSDETGAGSGEDDGQGSSQDEEMINAGSSINEGDFQSGEDSLIANEVHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG19</accession>
    <name>W5NG19_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 5 member 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Sodium-dependent multivitamin transporter that mediates the electrogenic transport of pantothenate, biotin, lipoate and iodide. Functions as a Na(+)-coupled substrate symporter where the stoichiometry of Na(+):substrate is 2:1, creating an electrochemical Na(+) gradient used as driving force for substrate uptake. Required for biotin and pantothenate uptake in the intestine across the brush border membrane. Plays a role in the maintenance of intestinal mucosa integrity, by providing the gut mucosa with biotin. Contributes to the luminal uptake of biotin and pantothenate into the brain across the blood-brain barrier.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-lipoate(out) + 2 Na(+)(out) = (R)-lipoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73379"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-pantothenate(out) + 2 Na(+)(out) = (R)-pantothenate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73371"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>biotin(out) + 2 Na(+)(out) = biotin(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73375"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>iodide(out) + 2 Na(+)(out) = iodide(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71207"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with PDZD11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG19"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019578"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019610.1">
        <property type="protein sequence ID" value="ENSLOCP00000019578.1"/>
        <property type="gene ID" value="ENSLOCG00000015895.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682482"/>
    <dbReference type="KEGG" id="loc:102682482"/>
    <dbReference type="CTD" id="563356"/>
    <dbReference type="eggNOG" id="KOG2349">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155731"/>
    <dbReference type="HOGENOM" id="CLU_018808_11_1_1"/>
    <dbReference type="InParanoid" id="W5NG19"/>
    <dbReference type="OMA" id="GWWGMRR"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015895">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008523">
        <property type="term" value="F:sodium-dependent multivitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015878">
        <property type="term" value="P:biotin transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015887">
        <property type="term" value="P:pantothenate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000011">
        <property type="entry name" value="sodium-dependent multivitamin transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF2">
        <property type="entry name" value="SODIUM-DEPENDENT MULTIVITAMIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="628" mass="68522" checksum="95B4E954CD211540" modified="2014-04-16" version="1">MAAMVEMRFTLVDYVIFALLLLLSSGIGLYYAFSGGRQRTMQEFLLADRSMSCLPVALSLLATFQSAVAILGVPAEIYTSGTQYWFLGTSYVLGLLIPAHIFIPMFYRLRLTSAYEYLELRFNKVVRLCGTITFIFQMVIYMGVVLYAPALALNAVTGFDLWGAVLTVGLVCTFYTALGGLKAVIWTDVFQTVVMFAGQLAVIVVGAYQAGGIEAVWKKAQEGNRISGLDLNPDPLERHTFWTLGVGGVFMMLALYGVNQAQVQRYLSSRTEKEAITSCYVVFPCQQIVLALGCLMGLVMYARYGEESPLASGYVKAKDQMVLYFVMDVLKDLPGLPGLFLACLFSGALSTISSAFNSLATVTMEDLIKPHFPDMTEYRATLLSKSLALGYGLICLGMAYIASQMGSVLQASLSIFGMIGGPLLGLFCLGMFFPWANPTGAVTGLVAGLVMAFWIGIGSFVSQTVGSDPLPPLNTTALPDTGNMTTVAMTTLVSAVANKARPTGLQHFYSLSYMWYSAHNSTTVVIVGLLVSFLTGPMKEKDVTPGTIYPVLGTLLFFLPERYREKLCCVTTLERRASEPSAQQQLMTQKTSNGNAFCTNEDKAEDEDLERRLGPVCHYTYIVHETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGU4</accession>
    <name>W5NGU4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amine oxidase</fullName>
            <ecNumber evidence="10">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01008591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGU4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019853"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019886.1">
        <property type="protein sequence ID" value="ENSLOCP00000019853.1"/>
        <property type="gene ID" value="ENSLOCG00000016112.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691383"/>
    <dbReference type="KEGG" id="loc:102691383"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166792"/>
    <dbReference type="HOGENOM" id="CLU_004498_0_4_1"/>
    <dbReference type="InParanoid" id="W5NGU4"/>
    <dbReference type="OMA" id="LWARVMH"/>
    <dbReference type="OrthoDB" id="7777654at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016112">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF14">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="11">
        <location>
            <begin position="13"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="536" mass="59269" checksum="EBC7695477A8FBCD" modified="2014-04-16" version="1">MELLDVVIVGAGLSGLTTARELLKRNAYLKILILEGKARIGGRTVTLSLPAAKGEDSWDLGGQWVGRTQTHILDLIQELGLEIYPQYTEGKKVYRLGGPEAEIRTYTSSIPPLSLMGLIDMQLLLWKIDKLRKTVSVEIPGSSPNALEYDSMTLHSFIKKNTWTQEVRAAMELSCRSVFGMETSQLSFLYFLMYGAAAGGFLALLEATAGSAQEYKVKGGTQQLSEILADRVGRQNIRLSSAVTAIHQDSGIVEVRTESEAFKCKSVIVTSPPHLASKIHYQPPLPVERESLTQNMPMGHMMKFIVTYPTTFWRENGFSGEIVTQPSSECPFSVTYDATSTSGNPALVGFIAGAQAADWCHRELEDRRDAVIQTLTKFLGPEASNYIHYAEKDWAKEQYSGGCPVNIMVPGMITYFHPSLRRPCGRIHWAGTETATLWCGYLSGAVQSGQRVALEVLGQLSPECLSQEEKEWARKSLAFPVPHRDWKIATGSSSFSAYLLLSTAALGAALLWVHPWFSQVLIERGLSILQKTYMMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHP9</accession>
    <name>W5NHP9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich PDZ-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cysteine-rich interactor of PDZ three</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRIPT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638697.1">
        <property type="nucleotide sequence ID" value="XM_006638634.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHP9"/>
    <dbReference type="FunCoup" id="W5NHP9">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020158"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020191.1">
        <property type="protein sequence ID" value="ENSLOCP00000020158.1"/>
        <property type="gene ID" value="ENSLOCG00000016330.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693968"/>
    <dbReference type="KEGG" id="loc:102693968"/>
    <dbReference type="CTD" id="9419"/>
    <dbReference type="eggNOG" id="KOG3476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183100"/>
    <dbReference type="HOGENOM" id="CLU_133934_0_0_1"/>
    <dbReference type="InParanoid" id="W5NHP9"/>
    <dbReference type="OMA" id="MPCDKCE"/>
    <dbReference type="OrthoDB" id="147332at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016330">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019367">
        <property type="entry name" value="PDZ-binding_CRIPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11805:SF1">
        <property type="entry name" value="CYSTEINE-RICH PDZ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10235">
        <property type="entry name" value="Cript"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="101" mass="11245" checksum="A96A3FC5E58665D7" modified="2014-04-16" version="1">MVCEKCEKKLGRVITPDTWKDGARNTTESGGRKLNENKALTSKKGRFDPYGKSRFAICRICKSSVHQPGSNYCQGCAYKKGICAMCGKKVLETKNYKQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHV8</accession>
    <name>W5NHV8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-lysine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.60</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the trimethylation of 'Lys-116' in calmodulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[calmodulin]-L-lysine + S-adenosyl-L-methionine = [calmodulin]-N(6)-methyl-L-lysine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21556"/>
            <dbReference type="Rhea" id="RHEA-COMP:11360"/>
            <dbReference type="Rhea" id="RHEA-COMP:11361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.60"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.60" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01001688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218806.1">
        <property type="nucleotide sequence ID" value="XM_015363320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHV8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020217"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020251.1">
        <property type="protein sequence ID" value="ENSLOCP00000020217.1"/>
        <property type="gene ID" value="ENSLOCG00000016374.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689830"/>
    <dbReference type="KEGG" id="loc:102689830"/>
    <dbReference type="CTD" id="79823"/>
    <dbReference type="eggNOG" id="KOG3201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002168"/>
    <dbReference type="HOGENOM" id="CLU_057006_0_0_1"/>
    <dbReference type="InParanoid" id="W5NHV8"/>
    <dbReference type="OMA" id="WYYLAPQ"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016374">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018025">
        <property type="term" value="F:calmodulin-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000140">
        <property type="entry name" value="Calmodulin-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025800">
        <property type="entry name" value="CaM-Lys-N-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539:SF3">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51610">
        <property type="entry name" value="SAM_CLNMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="336" mass="37927" checksum="DB9EF0AD7077CBF5" modified="2014-04-16" version="1">MEGNPTGGSGLCKNEELSGQAMSGEKDTSRQSDKHPVCRPDVARARWSLLSQVLKHKQLDNIEVQQVSVRRFSSFNLFTRTRVRDLEIEEPGDEMWFEYRSATFPQFRAFLRDNLGPIKVNEVLNSFDNTGNVCVWPSEEVLAYYCLKNRHMFRDVTVCELGGGMTCLAGLMIAISADAKEVLLSDGNEKAIQNVRKVIELNRKAGVFKTATVSSCVVRWDDESDVSALEGHFDIVICADCLFLDQYRASLVDAIKRLLKPNGSAIVLAPRRGGTLGEFCSLAERAGFSICRYENYDEHIWNLHSKMKSETSQSYDENLHYPVLLTLTWSRPQTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI32</accession>
    <name>W5NI32_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Thyroid peroxidase</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the peroxidase family. XPO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI32"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020291"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020325.1">
        <property type="protein sequence ID" value="ENSLOCP00000020291.1"/>
        <property type="gene ID" value="ENSLOCG00000016428.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158104"/>
    <dbReference type="HOGENOM" id="CLU_006087_1_0_1"/>
    <dbReference type="InParanoid" id="W5NI32"/>
    <dbReference type="OMA" id="FMAGDTR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004447">
        <property type="term" value="F:iodide peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09825">
        <property type="entry name" value="thyroid_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000001">
        <property type="entry name" value="Peroxidasin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029589">
        <property type="entry name" value="TPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF60">
        <property type="entry name" value="THYROID PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7 21" id="KW-0768">Sushi</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sushi" evidence="22">
        <location>
            <begin position="670"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="735" mass="82467" checksum="30AFAEB700230615" modified="2014-04-16" version="1">QILSFFKQAEPETQEISRAAELLETTLHILKSRTPKRNKRTIMIKDHLSMKDLKLIANLSGCLPYMQPPICPNGCLARKYRTISGACNNRNHSQWGAASTALARWLPAEYEDGRNQPKGWNAGLLYNGFPLPPVRDVSNNIMQTSNDQIVEDDSYSQMIVDWGQYIDHDISFTPQTTSKASFVEGVDCQNTCENLNPCFPIKIPPNDSFSGTRDCLPFFRSSPACFTGDEAVLFEDLKMLPQRQQMNALSSFLDASTVYGNSPSMVSKLRNFSSQEGLLAVNTKFTDSGRSYLPFVSKTPSPCAQDPRDAGGERIECFMAGDTRSSEVISLSVLHTLWVREHNRIAKALKKLNHHWTAETTYQETRKIIGALHQIITFRDYVPKILGADAFDQYIGVYGGYDSSLNPTVSNVFATAAFRFGHATVSPVTHRLDEKYQEHNNFSSVSLHETFFSPWRLIKEVGGLDPVIRGLMVNPAVVVTPDHLMNEELTKKLFVLSSPGALDLASLNLQRGRDHGLPGYNAWRKFCGFPTLESQADLHTVISNASIVQRIMDVYGHPSNIDVWLGGLVEDILPGARTGPLFACLIGKQMKMLQEGDWFWWENSGVFTQTQREEFEKHSLSRVICDNSGVTEVSLDPFRLGRYPEDFFPCDQIPGMNLDAWQENLNEERTQCGSPTKIEHGDFVFCSSPGKLVVMYSCYFGYRLEGAEEIFCTENGWSGDPPLCKGNGHLIHVTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI47</accession>
    <name>W5NI47_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TAFA chemokine like family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208438.1">
        <property type="nucleotide sequence ID" value="XM_015352952.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208439.1">
        <property type="nucleotide sequence ID" value="XM_015352953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI47"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020340.1">
        <property type="protein sequence ID" value="ENSLOCP00000020306.1"/>
        <property type="gene ID" value="ENSLOCG00000016441.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689607"/>
    <dbReference type="KEGG" id="loc:102689607"/>
    <dbReference type="CTD" id="338811"/>
    <dbReference type="eggNOG" id="ENOG502S0VQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161214"/>
    <dbReference type="HOGENOM" id="CLU_126078_1_0_1"/>
    <dbReference type="InParanoid" id="W5NI47"/>
    <dbReference type="OMA" id="ISWRARM"/>
    <dbReference type="OrthoDB" id="9924724at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016441">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051743">
        <property type="entry name" value="TAFA_chemokine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770:SF1">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867673" evidence="3">
        <location>
            <begin position="31"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="131" mass="14487" checksum="3040AE63887165A5" modified="2014-04-16" version="1">MSNRYTQKATKGKLLLIIYMISLWGKSVSAAGHHKVHHVKTGTCEVVAIHRCCNKNKIEERSQTIKCSCFPGQVAGTTRAAPSCVDASIVEQKWWCQMHPCLDGEECKVLPDLKGWSCATGNKVKTTKVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ56</accession>
    <name>W5NJ56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">ATP-binding cassette sub-family C member 10</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
            <ecNumber evidence="15">7.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Multidrug resistance-associated protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an S-substituted glutathione(in) + ATP + H2O = an S-substituted glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19121"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:19122"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Basal cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EC" id="7.6.2.3" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01001749">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020665"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020701.1">
        <property type="protein sequence ID" value="ENSLOCP00000020665.1"/>
        <property type="gene ID" value="ENSLOCG00000016723.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164531"/>
    <dbReference type="InParanoid" id="W5NJ56"/>
    <dbReference type="OMA" id="PYAWPSQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016723">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015711">
        <property type="term" value="P:organic anion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18598">
        <property type="entry name" value="ABC_6TM_MRP7_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18605">
        <property type="entry name" value="ABC_6TM_MRP7_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.1560.10:FF:000037">
        <property type="entry name" value="ATP-binding cassette subfamily C member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001090">
        <property type="entry name" value="ATP-binding cassette subfamily C member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC1_TM_dom"/>
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    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
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    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
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            <begin position="136"/>
            <end position="154"/>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="174"/>
            <end position="193"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="374"/>
            <end position="392"/>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="476"/>
            <end position="496"/>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="933"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1023"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1097"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1122"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1209"/>
            <end position="1228"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="1240"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="26">
        <location>
            <begin position="339"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="25">
        <location>
            <begin position="653"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="26">
        <location>
            <begin position="1014"/>
            <end position="1266"/>
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        <location>
            <begin position="1302"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="287"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="881"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="884"/>
            <end position="901"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004554"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00009726"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023055"/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00082787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1539" mass="167263" checksum="A3F33FBC0835DA84" modified="2014-04-16" version="1">IRSSFVGRSCSKLCHTDSLDPLPVWQNGTISPCFSQLFLGALAHSVLALSSACYLSTPSCAPAGGPPAWGWLCRAAAAALLALLFTADLAVSPLLRQPALYLDVLADGAAVLAWLVHCGALAALRRSAYGRGRGPPELLVSALLPGAALVVSLVSYLRDPGYLDPGRPLRLLRLAAASARLLLLCLYLLAWAAPGPGARASLSVNDSERAPLLAAPGPGEIVGEDGSGCISRLFYFWLNPLMQRGRRRELESYSDLYLLPRGLRTSAVREHFERCWEECRPAAQAAGPRELGRSAQKGAAGWPEPPEAPGGEGGPLSPPTPEVRLLRVLHAAFGTRYYLLGALKLAGNMLGFAGPLLLNSLVSFMEAGAAPLSRGVWCALGLFASTFLAAVLRNVFTFEVSKISLAARSAIISAIYRKALCVSGTTLARFTLGEVVNFMSTDTDRLVNFFGSFHEVWSLPVQFAITLYLLDQQVGVAFLGGLALALLLVPLNKVIASRIMENNKSMLKHKDSRVKLMTEVLSGMRVIKFYTWEKHFAEKIAGCRKQELAHLKTIKYLDAVCVYTWAALPVVISIITFITYVLLGHELTAAKVFTALALVGMLIMPLNNFPWVLNGTIEAKVSLDRIQRFLELSDQDLDAYYSSEVPEDPQTAIQLKHGAFSWQGPASRDPPAGGSLQLHSLDLTVRQGALVAVVGKVGCGKSSLLAAITGELNRCGGLLYVQGRELGFGLAAQEPWIQHASVRDNILFGKEHSARLYRAVLEACALPDDLSILPSGDLTEVGENGVTLSGGQKARLALARAVYMEKDIYLLDDPLAAVDADVASHLLEKCILGILKHKTRILCTHRTEFLDKADVVVLMDEGKIVKTGTPEEILPLVEAVPKTRKNDDNRKEKDGAGDEQRGLQPADQAPVEASLLGEEQKQSGVVSLRVYRAYWLAVGSCLALSILLSLLLMQASKNVSEWWLSNWVSHLKKTDNVSLAEGDPSLLHLSPRLLLFSLGGLLSPVPLMGSAPSPNSSSDLKYYLTVYGSIAAGNSVFTAIRAFLFAYGAVRAATAVHDRLLDRVLKATVTFFDTTPLGRIVNRFSSDLYSVDDTLPFILNILLANAFGLLGMLVVMSYGLPWILLVLLPLAGLYYLIQRYYRHSSRELKRLYSLTLSPIYTHFSETLTGLSTIRASRAAPRFEKENESRLERNQRCLFASNAAMQWLDIRLQMIGVAVVTGIAVIAVIQHQKKSVDPGLVGLSLSYALSITSLLSGLIFSFTQTETQMVSVERTEEYSTDTPIEPQGGCVEVPPSWPHRGQIEFQEAVLAYRAGLPNALDGVSVVIRPGEKVGIVGRTGSGKSTMFLALFRMVELSGGQILVDGVDTRLLGLTDLRSQLAVIPQDPFLFSGTVRENLDPRGRQPDAALLEVLEECHLTEAVARMGGLAAEVGEKGKSLSLGQRQLLCLARALLTQAKILCIDEATASVDQKTDRLLQQTIREKFREKTVLTIAHRINTIMDSDKVLVMHAGKVAEFESPSILCQREDSIFYRLVHGHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK55</accession>
    <name>W5NK55_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger CCCH-type containing 12D</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK55"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021014"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021050.1">
        <property type="protein sequence ID" value="ENSLOCP00000021014.1"/>
        <property type="gene ID" value="ENSLOCG00000017000.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155107"/>
    <dbReference type="HOGENOM" id="CLU_013020_2_1_1"/>
    <dbReference type="InParanoid" id="W5NK55"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017000">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF11">
        <property type="entry name" value="RIBONUCLEASE ZC3H12D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="287"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="517" mass="58080" checksum="1628572D12725C6B" modified="2014-04-16" version="1">MDRHQRTIERFLKLGYSRRDIVRVLESLHWDALTNDILEELLKTGSSAQPAGAPPRSPSPQLVPRGCSPAELAEEELPEPGSGLRPVVIDGSNVAMSHGDKQVFSCRGIQLAVQWFWDRGHHDITVFVPLWRKEQPKPEAPITHQHVLHELERRKILVYTPSRCVNGKRVVCYDDRFIVKLAYKSDGIIVSNDNYRDLQLEKPHWKKFIEERLLMYTFANDTFMPPDDPLGRNGPTIENFLRKTPQVPEHRWQHCPYGKKCTYGIKCKFYHPERSNQSHLSVADELRAKTRSTSPKAFPDAPAVAPRQDSARSGLSPSRQLESSSPRHSPSSPWDPPTFPRPASAGGLDSLEARISNMYVQGRPCWSGSPPPRSSSPGRPLPSHSHNHSMNCARACPGRGARTEIPRGPKPCEVVLPHGDPTPYQEQELEQYHYPGVPPKCSALPSFSWGGGQFGLLVGGQVSEPLLSEQKRTVRKQLCALFPQAAVDQVMCLHPNVLDTSSLISIIHKFRSSHYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKJ5</accession>
    <name>W5NKJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037873">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037874">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKJ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021154"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021190.1">
        <property type="protein sequence ID" value="ENSLOCP00000021154.1"/>
        <property type="gene ID" value="ENSLOCG00000017119.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXKJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286992"/>
    <dbReference type="HOGENOM" id="CLU_010468_3_1_1"/>
    <dbReference type="InParanoid" id="W5NKJ5"/>
    <dbReference type="OMA" id="MESAECE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017119">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF186">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="284" mass="31297" checksum="02CD05E0346B0B29" modified="2014-04-16" version="1">LTSQGSELRVVLLGKTGSGKSSSGNTILGRDEFKAECSSRSVTQWCQTGTSYIGGRRVTVVDTPGFFDTNQPEEDLRREIVRCVTECAPGPHAFLVVLEVRTYTPEEREAVERVQMQFGEEALKFTMVLFTHGEKLKHKSIEQFVQENEQLKGFVQKCGGGCHVFNNENRRDENQVTELVKKIEDMVWKNRGGHYTNEMLKAAQAAIEKEEECLRQESPDLDPSSIRKLAKERVAKKMLNILAGTATGALLGAFLGTVGPIVGHTVGGIVGPIVGHTVGHCGWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLK7</accession>
    <name>W5NLK7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637928.1">
        <property type="nucleotide sequence ID" value="XM_006637865.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLK7"/>
    <dbReference type="FunCoup" id="W5NLK7">
        <property type="interactions" value="1117"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021516"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021553.1">
        <property type="protein sequence ID" value="ENSLOCP00000021516.1"/>
        <property type="gene ID" value="ENSLOCG00000017411.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693316"/>
    <dbReference type="KEGG" id="loc:102693316"/>
    <dbReference type="CTD" id="84826"/>
    <dbReference type="eggNOG" id="ENOG502QV5D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018525"/>
    <dbReference type="HOGENOM" id="CLU_099529_3_1_1"/>
    <dbReference type="InParanoid" id="W5NLK7"/>
    <dbReference type="OMA" id="GLMFFTR"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017411">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="22641" checksum="583EE0593DBE6C26" modified="2014-04-16" version="1">MADLNRQLQEYLAQSKSGAKAGSQPSSSTAIDIGETSSVNGSWFGRWSSSFSGTANNSAGQGSSSGVSWPWSSEPDACLPGMSRSQRLVAFGVCIAISVLCFGLSALYAPLLLLKARKFALLWSLGSVFALLGAGILRGPSKLLTTPSPGAALYLCSLTGTLYAALSLHSTALTALGAVVQVAAIAGYIIALLPGGTAGMKFLGSMAASAIKRTVSSKTMPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM72</accession>
    <name>W5NM72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DIRAS family, GTP-binding RAS-like 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Displays low GTPase activity and exists predominantly in the GTP-bound form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the small GTPase superfamily. Di-Ras family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011289">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221008.1">
        <property type="nucleotide sequence ID" value="XM_015365522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NM72"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021731"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021768.1">
        <property type="protein sequence ID" value="ENSLOCP00000021731.1"/>
        <property type="gene ID" value="ENSLOCG00000017626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685357"/>
    <dbReference type="KEGG" id="loc:102685357"/>
    <dbReference type="CTD" id="327577"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159915"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="W5NM72"/>
    <dbReference type="OMA" id="LGPIYQL"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017626">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000303">
        <property type="entry name" value="GTP-binding protein Di-Ras2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="198" mass="22343" checksum="6B4A915606F7C9BA" modified="2014-04-16" version="1">MPEQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRDTYIPTIEDTYRQVISCDKSVCTLQITDTTGSHQFPAMQRLSISKGHAFILVYSITSRQSLEELKPIYQQVLQIKGSVENIPVMLVGNKCDETQREVETKEGEAQAAAWKCAFMETSAKMNYNVKELFQELLNLEKKRNMSLNIDGKRSSKQKRADKIKGKCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM92</accession>
    <name>W5NM92_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NM92"/>
    <dbReference type="FunCoup" id="W5NM92">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021751"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021788.1">
        <property type="protein sequence ID" value="ENSLOCP00000021751.1"/>
        <property type="gene ID" value="ENSLOCG00000017646.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686247"/>
    <dbReference type="KEGG" id="loc:102686247"/>
    <dbReference type="eggNOG" id="ENOG502RP7I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NM92"/>
    <dbReference type="OMA" id="YHIVITN"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="48"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="325" mass="36801" checksum="BAEF1599CC927059" modified="2014-04-16" version="1">MSTLNSVMSANATFVRPQFFYINGFSGIPHAQYYYVFLCFVYAVTVLANAFILFIIYIEQSLHTPKYMAVFNLAVVDMCGSTALIPKMIETFVFDSQFITYEACIANMFFVHFFFFLESLTLVVLAYDRFVAICFPLKYHIVITNSQMAVILAVVWFLTSGLVITCVGFITTLSFCKTIVIDSYFCDHGPMYRMACNNNFPSYIMAKIAITVLLFAPLVLILLSYVCIIFALLKIASGEQRLKAMKTCTSHLLLVTVFYLPISATYIAAIMSTLHPNTRIISTSLSSTIPPMLNPIIYTIKTEEIMTAIKKRYKRNKTITATNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM94</accession>
    <name>W5NM94_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 51F2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NM94"/>
    <dbReference type="FunCoup" id="W5NM94">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021753"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021790.1">
        <property type="protein sequence ID" value="ENSLOCP00000021753.1"/>
        <property type="gene ID" value="ENSLOCG00000017648.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687465"/>
    <dbReference type="KEGG" id="loc:102687465"/>
    <dbReference type="eggNOG" id="ENOG502RP7I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NM94"/>
    <dbReference type="OMA" id="HIMMGKL"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:odorant binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
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    </dbReference>
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        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF966">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="127"/>
        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="232"/>
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    </feature>
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            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="325" mass="36599" checksum="81A9F86C032C271B" modified="2014-04-16" version="1">MSSLNSTLPQNVSFVRPQFFYINGFSNIPYVKYYYIFLCIVLTVTILGNSFIMFIIYIEQSLHTPKYVVVFHLAVVDMCGSTALIPKMIETFLFDSQLISYEGCLANMFFVHSFTFMQSLTLLALAYDRFVAICFPLKYHIIITTAGMGKILAGIWILASGVVIAAVGFTTRLSFCKSIVINSYFCDHGPIYRMSCNDNFPNYVIAQIGITILLAAPLTLILSTYVCIILALLKIASEEERIKAMKTCTSHLILVAMFYLPISAIYIAANITSIDPNTRIISTSLSSTISPMMNPIIYTMKTEEVMKAVRKLCKRNKIIKPRRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMY2</accession>
    <name>W5NMY2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Trace amine associated receptor 12f</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026009">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000021991"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022030.1">
        <property type="protein sequence ID" value="ENSLOCP00000021991.1"/>
        <property type="gene ID" value="ENSLOCG00000017888.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_009579_11_0_1"/>
    <dbReference type="InParanoid" id="W5NMY2"/>
    <dbReference type="OMA" id="LIFNRQW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15312">
        <property type="entry name" value="7tmA_TAAR2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009133">
        <property type="entry name" value="TAAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF220">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01831">
        <property type="entry name" value="TRACEAMINE1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="347" mass="39230" checksum="4D29B9819D495443" modified="2014-04-16" version="1">FIMNSTTEFEEMQYCFQYLEGSCPKAHRTAVIKVAMYIFMVGTMLATVSGNLMVIISIAHFKQLHSPTNFLTLSLGCADCLLGAFVMPYSMVRSVETCWYFGDLFCKIHSSLDMTISVASILHLTFIAVDRYVAICDPLRYRSKITTFVVAIFIGISWIYSLGLGFGVVFSKINLTGIEEFVILNSCVGTCFLIFNKHWGVLAALLAFLIPGTVMISLYVKIFMVASRHVRVLNDTSGKICARNDHKRKIVENRERKAAKTLGIVMGVFLICWLPFFMATIIDPFIGFSTPVILFDALVWFGYFNSTCNPVIYGFFYPWFQKAFKIIITGKVFCYNSSSTHLFTDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN77</accession>
    <name>W5NN77_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Prion protein, related sequence 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031440">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198627.1">
        <property type="nucleotide sequence ID" value="XM_015343141.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN77"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022086"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022127.1">
        <property type="protein sequence ID" value="ENSLOCP00000022086.1"/>
        <property type="gene ID" value="ENSLOCG00000017985.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690852"/>
    <dbReference type="KEGG" id="loc:102690852"/>
    <dbReference type="eggNOG" id="ENOG502QTSK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064873"/>
    <dbReference type="HOGENOM" id="CLU_042933_0_0_1"/>
    <dbReference type="InParanoid" id="W5NN77"/>
    <dbReference type="OMA" id="RCVEEYM"/>
    <dbReference type="OrthoDB" id="7617494at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017985">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0012506">
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    </dbReference>
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        <property type="term" value="F:phosphatidylserine binding"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Prion/Doppel_b-ribbon_dom_sf"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004870019" evidence="2">
        <location>
            <begin position="27"/>
            <end position="523"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="523" mass="54354" checksum="EF16E2B6AC3269C1" modified="2014-04-16" version="1">MLRPAVRLSLLALLLLLGLHCDPAWGKGRGGGSRGGGSKSSGSKGSSWNFGKSKTGQTGSTGQTRNTGTAAGKPNPGSYPKQQYPAGGYPQQYPNQNPGGAGANPGGYPNQYPAGGYPNQNPGRAGANPGGYPNQYPAGGYPNQYPNQNPGRAGANPGGYPNQYPAGGYPNQYPGGYPNQYPARGGVNPGGYPNQYPAAGGYPHAYPGGAGYPGGGQGWGQPAGYPNWNPNNKFPSPRFGGYGHGAGGYGAGGSPFSRTAQDMGYGPSHKSKGFGKKAAMAAGVGAVAGMAVGYGLGRFPRPNFNFHSPEEAHYYNHYMWRRYGSRSTDENDYGRDYQYNPPPQGYDSFMDNCIKRPDLLPQPEATPPPASDGPAPGHTPGNAPGNPGAEPNGTLPSQVNNSTANGTQAVSDAGLAMPSPQGTGPDGEGPGIPSPSLGEQEQDDNDTVSIMEIGYPELIEQLKVRRCVEMYITYSDSYLQKQTDTRGAPNPKGAGTQQLPLCQGLVLLVTTSLTLLTSTLLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNZ9</accession>
    <name>W5NNZ9_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNZ9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022399.1">
        <property type="protein sequence ID" value="ENSLOCP00000022358.1"/>
        <property type="gene ID" value="ENSLOCG00000018257.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SYXD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067810"/>
    <dbReference type="HOGENOM" id="CLU_1869812_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNZ9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018257">
        <property type="expression patterns" value="Expressed in ovary and 4 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="121" mass="13602" checksum="8A9287416E0559AB" modified="2014-04-16" version="1">WPPAMNYSYLCAHPASHPFPSPYSAATSPPTWLDHGPFLPPIHGYPPRPLTLVSQDWGGGHVEYHHFYGLNPLALLSPYWPWPYSQPARPQGYGPGRSQAQIEGSWPEGFTLRGELRWGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP17</accession>
    <name>W5NP17_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligodendrocyte transcription factor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP17"/>
    <dbReference type="FunCoup" id="W5NP17">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022376"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022417.1">
        <property type="protein sequence ID" value="ENSLOCP00000022376.1"/>
        <property type="gene ID" value="ENSLOCG00000018275.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3898">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166262"/>
    <dbReference type="HOGENOM" id="CLU_065376_1_0_1"/>
    <dbReference type="InParanoid" id="W5NP17"/>
    <dbReference type="OMA" id="YCADRET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018275">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000031">
        <property type="entry name" value="Oligodendrocyte transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF161">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="87"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="254" mass="27304" checksum="4853E54B621EB7D9" modified="2014-04-16" version="1">MDSDAGSASSRSSSPEFVEEARAFFSNRMFRAYCRDQEQELEPEQEQEQPRTSLLPKEGSSGAGAGAGGAGGRAKPKRGLTEDDLQGLRLKVNSRERKRMHDLNQAMDGLREVMPYAQGPSVRKLSKISTLLLARNYILMLSSSLEEMKKLVSDVYGGSHPSHVPRCSPVGTSPAQLPLHPLAQSLHSLVGTTSTATHSSPSAGYLGFRPPLQTLLKELSSPSNLGVSYRHLTGMPCPCSLCQPLPASTASLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVC2</accession>
    <name>W5LVC2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVC2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000079"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000079.1">
        <property type="protein sequence ID" value="ENSLOCP00000000079.1"/>
        <property type="gene ID" value="ENSLOCG00000000069.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006605_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVC2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000069">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="277" mass="30506" checksum="776B89EB8C7671F8" modified="2014-04-16" version="1">VPARPVAGLVGDELHLGLLQGVGHLTCTGRGGRAPHNLTTTPRNLTRTKHPTISPNNTPKISQHPKISLTQSTLKSSHNTSKSHSPRVPRNLTKTIPPKTHNTPQSHHSTPKYHSNRAPHNLTIAPRNPTHTEHPTISQHPEISLTKSTPKPHSHRAPHNLTTTPRNLTYTEHPTISPQHPETPLTESTPKSHHNTPKSHSHRAPHSLTTAPQNPTHTEHPTISPQHPEISLTQSTPQSHQTIPAKSHNTPKSHSHRTPHNLTTPQNLTRTEHPTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVF1</accession>
    <name>W5LVF1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA 3'-terminal phosphate cyclase</fullName>
            <ecNumber evidence="2">6.5.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA terminal phosphate cyclase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. Likely functions in some aspects of cellular RNA processing. Function plays an important role in regulating axon regeneration by inhibiting central nervous system (CNS) axon regeneration following optic nerve injury.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="W5LVF1"/>
    <dbReference type="FunCoup" id="W5LVF1">
        <property type="interactions" value="650"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000108"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000108.1">
        <property type="protein sequence ID" value="ENSLOCP00000000108.1"/>
        <property type="gene ID" value="ENSLOCG00000000093.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063404"/>
    <dbReference type="HOGENOM" id="CLU_027882_0_1_1"/>
    <dbReference type="InParanoid" id="W5LVF1"/>
    <dbReference type="OMA" id="WSPPIDY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000093">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000002">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, RPTC, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="12">
        <location>
            <begin position="187"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="9">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="296"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005378-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005378-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="367" mass="39036" checksum="4DC2AE431FB2ABD0" modified="2014-04-16" version="1">ANMAATTFAIDGSIMEGGGQILRVSAALSCVQGASLSISKIRAGRSTPGLRPQHLSGLELVRDLCGGHLEGADVGSTEVALSSGKIKGGSHVADTKTAGSVGLLLQVSLPCVLFADAPSELCLKGGTNAEMAPQIDYTMKVFRPMVEKFGVKFDCELKMRGYYPRGGGEVIVKTNPVKELSPASLIERGSITKIYGRAFVAGVLPHKLAKDMATAAVRQIRKEIKDLYVNIQAMQEKKEEAFGNGNGIIIFAESSTGCVFAGSALGKKGVYADKVGIEAAEMLLRNVRHNGCVDEFLQDQLIVFMALANGTSRVRTGPVTLHTRTAIHVAEQLTKAKFTITKAEDQNSTNETHIIECQGVGMTNPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW73</accession>
    <name>W5LW73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">von Willebrand factor A domain containing 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LW73"/>
    <dbReference type="FunCoup" id="W5LW73">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000380"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000380.1">
        <property type="protein sequence ID" value="ENSLOCP00000000380.1"/>
        <property type="gene ID" value="ENSLOCG00000000347.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011517"/>
    <dbReference type="HOGENOM" id="CLU_504868_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW73"/>
    <dbReference type="OMA" id="LMMGIAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000347">
        <property type="expression patterns" value="Expressed in pharyngeal gill"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056475">
        <property type="entry name" value="GBD_Hemicentin/VWA7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052577">
        <property type="entry name" value="VWA7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056862">
        <property type="entry name" value="VWA7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905">
        <property type="entry name" value="NG37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF18">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING 10, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23560">
        <property type="entry name" value="GBD_Hemicentin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25107">
        <property type="entry name" value="VWA7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWA7 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Hemicentin-1-like von Willebrand factor A" evidence="6">
        <location>
            <begin position="222"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Hemicentin/VWA7 galactose-binding" evidence="5">
        <location>
            <begin position="408"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="54922" checksum="689F6F27DD8D5276" modified="2014-04-16" version="1">SSETFEEAIQSIVLSNAWVDLRHVLNEEYHFDNESFLKGKKLITDGIASVKGNIRQQNFYTARKRLGQIFHTLQDFYSHSNWIELGYKTPFSNLIQSSASLENIASKGEPTCSDCAEGTCPDNTLESILQGRKLTSGYFDLRPWASKPTGKCSHGGLSDFTSDDIPRGGISKDTYSSNHGSLHEIAAQIATTATMEQLQDLRNAAGDDEFLRLMGISRPSVLCFVIDTTGSMFDDIEEAKRRSFSIIDTKRGTSNEPSAYILVPFNDPDFGPVTRTKDPEVFKKKINALTASGGGDEPEMCLSALQEKPSPVPPPSSEIYVFTDASAKDSYLKSTIIALIESTKSVVSFLLTAGLSRRRRWSSSSRLSQDAALLYQDLAQTSGGQAVRVSKGQLSEASAIISSSAALVTLFQSVTDPGKTEEIFSFYVDPSVSNLTLYITGSFLNFKLHSPAGLSQNSSVIEGPLGRLHAVGNLYTVYLENQTGLWEINVTSSRPCSIKVTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWV6</accession>
    <name>W5LWV6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SREBF chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SCAP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWV6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000613"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000614.1">
        <property type="protein sequence ID" value="ENSLOCP00000000613.1"/>
        <property type="gene ID" value="ENSLOCG00000000555.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158130"/>
    <dbReference type="HOGENOM" id="CLU_450992_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWV6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000555">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000391">
        <property type="entry name" value="sterol regulatory element-binding protein cleavage-activating protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057042">
        <property type="entry name" value="Beta-prop_SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24017">
        <property type="entry name" value="Beta-prop_SCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="554"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="SCAP beta-propeller" evidence="9">
        <location>
            <begin position="230"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="606" mass="65592" checksum="AA2EBDF9E69C0A92" modified="2014-04-16" version="1">MAGTVIWIGILVYTDPAGLRTYLAAQVSEQSPLGEAGLPHLAVPPVFPGGDPTSTLSVLPPPEPTPLPENHSRGHGPPSQPSSDPQITWGPEDEETWRRLSFRHWPSLFSYYNITLAKRYISILPVIPVSLYLSAHDALEARHPQDTRHPQPPPASPAPPSTQADLTLYKVAALGLAAGVLLVIILFCLYRVLCPRNYGQNGVSHGRRRRGDLPCDDYGYSPPVSEISPLLLRGHNMDIECLASDGMLLASCCLAGQIRVWDAQTGDCLTVIPNHGLRRSSSGAWEQRDGWDGGSVFEPEVSDGYEGQEAGGGVPAPGGTGYPVRRRVTPSRLVLFGDQPDLTPFIDTNFTEQPPPLCPTPSGGFDFGELVQRAYKEHEASAGLPGYPPSPPTPLQRRRSLGDQPLPPPELPSPVPLDWDSAVWALELRGNLIAAGRSSGKLELWDAVEGCLRCCNEDGTSGITALAFLNNRIVAARLNGSLDFFTVDINKPPCLLQYRGTPNRGSMPPSPCYSSDDVISCQLTRSVQCAHQKPITVLRAAAGRVVTGSQDHTVRVRMIIIIIAYTYIALFWTLHSKRFTGNGVSPPPPPMCSIHLDDATAAIVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXI0</accession>
    <name>W5LXI0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BAAT/Acyl-CoA thioester hydrolase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000837"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000841.1">
        <property type="protein sequence ID" value="ENSLOCP00000000837.1"/>
        <property type="gene ID" value="ENSLOCG00000000756.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ8Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222336"/>
    <dbReference type="InParanoid" id="W5LXI0"/>
    <dbReference type="OMA" id="CCPETGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
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        <property type="term" value="P:fatty acid metabolic process"/>
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        <property type="entry name" value="Acyl-CoA thioesterase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014940">
        <property type="entry name" value="BAAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824:SF38">
        <property type="entry name" value="ACOT1 THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08840">
        <property type="entry name" value="BAAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAAT/Acyl-CoA thioester hydrolase C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="23827" checksum="4B3B16630C09B49A" modified="2014-04-16" version="1">XLEYFEEALHFFQALPQVRENSVGVLGLSKGADLALSMASFLSGISAVVSVSGCNANTISLLHYKDTTLQRVTYSSEKIKVDQASGLWNFSEAYDDPRDPGCQAVVPVERASARFLFVVGEDDKVWNASLYASEAAKRLISHGKERPEVLSFPGTGHIIEPPYFPHGHSSFHKVINAPVLCGGQAESHAFSQEEAWRRIRQFFLDNLGAAGCRQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY66</accession>
    <name>W5LY66_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger, MYND-type containing 19</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LY66"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001073"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001077.1">
        <property type="protein sequence ID" value="ENSLOCP00000001073.1"/>
        <property type="gene ID" value="ENSLOCG00000000957.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUSS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153820"/>
    <dbReference type="HOGENOM" id="CLU_084232_0_0_1"/>
    <dbReference type="InParanoid" id="W5LY66"/>
    <dbReference type="OMA" id="FYECHYP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000957">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="His-Me_finger_sf"/>
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    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
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        <property type="entry name" value="ZMYND19"/>
    </dbReference>
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        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46831">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 19"/>
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    </dbReference>
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        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 19"/>
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    </dbReference>
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        <property type="entry name" value="HNH_3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="229" mass="26657" checksum="2DD2103619F5B6D5" modified="2014-04-16" version="1">CPMSDFKLGIVRLGRVAGKTKYTLIDEQDIPLVENYAFEARMEVDADGNGAKIFAYAFDINKGRWAGRPLHELLWEKHRGGIAPCFQVVHINSVTVDNRLDNLRLVPLGWTPKPEELSSKQREQSLYWLAIQQVPADPVEEQYLELSRTRYYNANGELVEEEECSCTYYECHYPPCSLIERRLREFNICGRCQVARYCGSQCQQRDWPAHKKQCRERKRVLALESEPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYJ3</accession>
    <name>W5LYJ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">TBL1X/Y related 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat EBI family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637468.1">
        <property type="nucleotide sequence ID" value="XM_006637405.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216286.1">
        <property type="nucleotide sequence ID" value="XM_015360800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYJ3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001200"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001204.1">
        <property type="protein sequence ID" value="ENSLOCP00000001200.1"/>
        <property type="gene ID" value="ENSLOCG00000001063.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684670"/>
    <dbReference type="KEGG" id="loc:102684670"/>
    <dbReference type="CTD" id="553608"/>
    <dbReference type="eggNOG" id="KOG0273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153421"/>
    <dbReference type="HOGENOM" id="CLU_007609_2_0_1"/>
    <dbReference type="InParanoid" id="W5LYJ3"/>
    <dbReference type="OMA" id="GENQPIK"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001063">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002234">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF40">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="349"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="391"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="442"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001200.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="518" mass="55953" checksum="9602474D508423FA" modified="2014-04-16" version="1">MSISSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGALVPPAALISIIQKGLQYVEAEVSINEDGTLFDGRPIESLSLIDAVMPDVVQTRQQAYRDKLAQQQAAAAAAAAAAASATANQQGVAKNGENTANGEENGAHALPNNHADMMEVDGDVEIPQNKAMVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLSENSTGSSTQLVLRHCIREGGQDVPSNKDVTSLDWNSEGTLLATGSYDGFARIWTKDGNLASTLGQHKGPIFALKWNKKGNFILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQSNNTFASCSTDMCIHVCKLGQDRPIKTFQGHTNEVNAIKWDPTGNLLASCSDDMTLKIWSMKQDTCVHDLQAHNKEIYTIKWSPTGPGTNNPNASLMLASASFDSTVRLWDVDRGICIHTLTKHQEPVYSVAFSPDGRYLASGSFDKCVHIWNTQTGALVHSYRGTGGIFEVCWNAAGDKVGASASDGSVCVLDLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M009</accession>
    <name>W5M009_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Melanotransferrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M009"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001716"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001721.1">
        <property type="protein sequence ID" value="ENSLOCP00000001716.1"/>
        <property type="gene ID" value="ENSLOCG00000001493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697447"/>
    <dbReference type="KEGG" id="loc:102697447"/>
    <dbReference type="CTD" id="4241"/>
    <dbReference type="eggNOG" id="ENOG502QSZB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159265"/>
    <dbReference type="HOGENOM" id="CLU_011309_3_0_1"/>
    <dbReference type="InParanoid" id="W5M009"/>
    <dbReference type="OMA" id="CPVPAMT"/>
    <dbReference type="OrthoDB" id="9981115at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001493">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000108">
        <property type="entry name" value="melanotransferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018195">
        <property type="entry name" value="Transferrin_Fe_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF21">
        <property type="entry name" value="MELANOTRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00205">
        <property type="entry name" value="TRANSFERRIN_LIKE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00206">
        <property type="entry name" value="TRANSFERRIN_LIKE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00207">
        <property type="entry name" value="TRANSFERRIN_LIKE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 19 21" id="KW-0408">Iron</keyword>
    <keyword evidence="5 19" id="KW-0410">Iron transport</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 24" id="KW-0732">Signal</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867059" description="Melanotransferrin" evidence="24">
        <location>
            <begin position="20"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="25">
        <location>
            <begin position="23"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="25">
        <location>
            <begin position="360"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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            <begin position="131"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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            <begin position="187"/>
            <end position="200"/>
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    <feature type="disulfide bond" evidence="22">
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            <begin position="258"/>
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    </feature>
    <feature type="disulfide bond" evidence="22">
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
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            <begin position="373"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="470"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="498"/>
            <end position="700"/>
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    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="512"/>
            <end position="529"/>
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    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="597"/>
            <end position="611"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054140"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072985"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR002549"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-2"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-3"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR002549-4"/>
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    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="726" mass="79034" checksum="D7BF1209777CB00E" modified="2014-04-16" version="1">MIAWTTICWGLITLQAVVGLSTVRWCTVSDPERKKCEDLSKAFREGGVRPELRCVTGASALDCAAKVSGNQADAVTLSGKDIYEAGKQYKFKTAVAETYGEEVGTTYYAVAVVWRNSTGININSLAGRRSCHTGLGRTAGWNMPLGLLIDSGRMSVMGCDIKRGVSEFFSASCVPGANGYGDPPGLCQLCVGDVSGNFKCESNEKERYYSYAGAFRCLAEGTGEVAFVKHSTVRENTDGRNPDPWAQALKSASFQLLCRDGSLAEVRDYRRCHLVRVPAKAVITRADTSSAELHRMLEEGRNKFQMFQSSSYGGTNLMFSDATKQFLLAESENYQDWLGQGYINALKGMDCTKDSVPKNLRWCVLSHPEMTKCAQMAVSFQKRGLTPTIQCVSGDSYEDCIVKIKNNDADAITLDGGFIYKAGKDHGLVPAVGESYTDDTQGSSYYAVAVVKKSSLDAFNFNELKGRRSCHTGYGRTAGWNVPVGVLMEKGMIRPRGCQFAQAAGEFFRSSCVPGANQPGYPPSLCQLCVGDVSGQHKCDRSDLERYYGYSGAFRCLSESAGEVAFVKHTTVFDNTDGHNTDLWAVNLQSRDFQLLCPNGARAEVSQYARCNLARVPAHAVMVRPDTDANAVYGLLDRAQAIFGDDNNPNGFKMFDSSAFGGTDLIFKDSTVRLIGVGEKRTYTDWLGQSYMDAMNVMECNSAGAVSSVSTVLVVGLGFLLIIFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0B9</accession>
    <name>W5M0B9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Disabled homolog 2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0B9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001826"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001831.1">
        <property type="protein sequence ID" value="ENSLOCP00000001826.1"/>
        <property type="gene ID" value="ENSLOCG00000001592.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107079662"/>
    <dbReference type="eggNOG" id="KOG3535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155567"/>
    <dbReference type="HOGENOM" id="CLU_093514_1_0_1"/>
    <dbReference type="InParanoid" id="W5M0B9"/>
    <dbReference type="OMA" id="RINSTCA"/>
    <dbReference type="OrthoDB" id="10069833at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001592">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048559">
        <property type="entry name" value="DAB1/2_SBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47695">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47695:SF3">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21792">
        <property type="entry name" value="DAB2_SBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="1">
        <location>
            <begin position="44"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="182" mass="20838" checksum="4D8753DEFDE1943B" modified="2014-04-16" version="1">MATEQEQTPPLSAPVQNHNPIRIRRTSKKVTDKTPAFLMARFQGDGVRYKAKLIGVDDVPEPQGDKMCLDSMMKLKGREVAARQQGQHKQRIWLKVSTAGVKIMDEKSGQVELEHPMEQISLVKKDEFDPRAFAYIFGHEGTYKLFFIKMANAADPVIEDFKDVRQIVPLTMLNRVRDRRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0P4</accession>
    <name>W5M0P4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01028788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0P4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001951"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001956.1">
        <property type="protein sequence ID" value="ENSLOCP00000001951.1"/>
        <property type="gene ID" value="ENSLOCG00000001696.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158897"/>
    <dbReference type="HOGENOM" id="CLU_001357_0_2_1"/>
    <dbReference type="InParanoid" id="W5M0P4"/>
    <dbReference type="OMA" id="VCAGPCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001696">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000012">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000018">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF11">
        <property type="entry name" value="PAXILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="51"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="111"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="19374" checksum="D62D7C1FDBA8C75A" modified="2014-04-16" version="1">LLQKVVTALDRTWHPEHFFCAQCGSFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNALWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMGKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQHCFVKLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M156</accession>
    <name>W5M156_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alpha-L-fucosidase</fullName>
            <ecNumber evidence="13">3.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 13">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:0) + H2O = a neolactoside nLc4Cer(d18:0) + L-fucose</text>
            <dbReference type="Rhea" id="RHEA:49308"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:91119"/>
            <dbReference type="ChEBI" id="CHEBI:91121"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:49309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:1(4E)) + H2O = a neolactoside nLc4Cer(d18:1(4E)) + L-fucose</text>
            <dbReference type="Rhea" id="RHEA:48224"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:28691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:48225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-L-fucoside + H2O = L-fucose + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:12288"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28349"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="EC" id="3.2.1.51"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01009424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M156"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002113"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002118.1">
        <property type="protein sequence ID" value="ENSLOCP00000002113.1"/>
        <property type="gene ID" value="ENSLOCG00000001807.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3340">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000035378"/>
    <dbReference type="HOGENOM" id="CLU_002934_1_1_1"/>
    <dbReference type="InParanoid" id="W5M156"/>
    <dbReference type="OMA" id="LPEHKWE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001807">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000051">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000027">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031919">
        <property type="entry name" value="Fucosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018526">
        <property type="entry name" value="Glyco_hydro_29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF2">
        <property type="entry name" value="TISSUE ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16757">
        <property type="entry name" value="Fucosidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001092">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00741">
        <property type="entry name" value="GLHYDRLASE29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00385">
        <property type="entry name" value="ALPHA_L_FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="15">
        <location>
            <begin position="30"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-fucosidase C-terminal" evidence="16">
        <location>
            <begin position="379"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="site" description="May be important for catalysis" evidence="14">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="52894" checksum="4EAC552F62BFB3CD" modified="2014-04-16" version="1">LTFVTEVPFQTTMRASRLLYLSCLLVSAVRSRYAPDWASLDSRPLPSWYDEAKFGIFVHWGVFSVPGFGSEWFWWHWRGTQEPLYVDFMEKNYPPGFAYPDFAPEFRAQFFDPEQWADVFEASGAKYVVLTAKHHEGFTNWGSAESWNWNAVDTGPHRDLVGSLGSALSKRSLRYGLYHSLFEWFHPLYLADKKAAFKTQEFVFRKTLPELYDLVHLLSSSRYQPDLIWSDGDWEAPDTYWNSTQFLAWLYNDSPVRDSVVTNDRWGAGCACKHGGYFNCEDKYTPGRLPEHKWEKCTSVDTLSWGYRRDMTRDQLLDLPAIIADLVRTVSLGGNYLLNVGPTADGTLPVAFEERLRGVGGWLAVNGEAVYSSRPWRVQNENATVPLWYTSKGSDVYAIFLEWPVDNVLQLGAPKTSPASNVTLLGYPKPLRWMPRGPTGLSVFLPVLRTPLAVGWTLRLQGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1R4</accession>
    <name>W5M1R4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribonucleoside-diphosphate reductase subunit M2</fullName>
            <ecNumber evidence="4">1.17.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ribonucleotide reductase small chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ribonucleotide reductase small subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01009429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1R4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002322"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002327.1">
        <property type="protein sequence ID" value="ENSLOCP00000002322.1"/>
        <property type="gene ID" value="ENSLOCG00000002000.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013305"/>
    <dbReference type="HOGENOM" id="CLU_035339_2_2_1"/>
    <dbReference type="InParanoid" id="W5M1R4"/>
    <dbReference type="OMA" id="WISALPC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002000">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF20">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="30506" checksum="6C2828776101D39B" modified="2014-04-16" version="1">RLQDEPLLRENPRRFVIFPIQYHDIWQMYKKAEASFWTAEEVDLSKDLQHWESLKQEEKYFISHVLAFFAASDGIVNENLCRAFGLSCIPMRLAFGPGRVLNTRIAFPIFAVLLAASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFRHLVHKPSEARVKQVVADAVAIEQEFLTQALPVNLIGMNCALMKRYIEFVADRLLLELGFSKLFKVENPFDFMENISLEGKTNFFEKRVGEYQKMGVMSKTVDNTFTLDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M265</accession>
    <name>W5M265_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Laminin, gamma 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000002479.1">
        <property type="protein sequence ID" value="ENSLOCP00000002473.1"/>
        <property type="gene ID" value="ENSLOCG00000002091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688384"/>
    <dbReference type="KEGG" id="loc:102688384"/>
    <dbReference type="CTD" id="3915"/>
    <dbReference type="GeneTree" id="ENSGT00940000166185"/>
    <dbReference type="HOGENOM" id="CLU_002471_1_0_1"/>
    <dbReference type="OrthoDB" id="430826at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002091">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000067">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000193">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000758">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000018">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000105">
        <property type="entry name" value="laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000163">
        <property type="entry name" value="laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000166">
        <property type="entry name" value="laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000174">
        <property type="entry name" value="Laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000769">
        <property type="entry name" value="Laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF270">
        <property type="entry name" value="LAMININ SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865444" evidence="16">
        <location>
            <begin position="21"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="19">
        <location>
            <begin position="31"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="383"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="430"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="18">
        <location>
            <begin position="506"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="709"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="813"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="869"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="920"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="968"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1020"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1116"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1397"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1543"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="892"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="920"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="922"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="941"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="968"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="988"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1594" mass="176530" checksum="A6C2203FDEB6560B" modified="2014-04-16" version="1">MHWFTKVLTILTCLATCSNGAMDECTDDLNRPQRCMPEFVNAAFNVTVVATNTCGSPPEEYCVQTGVTGVTKSCHICDARDPRVYHGAVYLTDYNNQADTTWWQSQTMLAGIQYPNTINLTLHLGKSFDITYVRLKFHTSRPESFAIYKRTSEDGPWVPYQYYSSSCEKTYQKQNRGFIRTGEDEQQALCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCNGHASECIKNVNGRLVCNCKHNTEGDDCSVCKGFYNDRPWRRATAENPNECLPCNCNGKSSECYFDPELYRATGHGGHCRGCADNTDGPNCERCRDNYYRQRPSDRCLPCSCNAVGSLSTQCDNSGRCSCKPGVMGDKCDRCQPGYHTLTEAGCRPCSCHSAGSTQECDIQTGRCQCKENVEGFSCDRCKLGYFNLDPLNPRGCTACFCFQHSSVCDSADGYSLHTITSTFSRDDEGWTGQQREGSSVKVQWLPSQQEISLISEDYFPIYFVAPEQFLGNQFLSYGQNISLSFRVDRRDTRLSAEDIVLEGAGLRVAVPLIAQGNAYPSENKQTYVFRLHDATDYPWRPSLSHSEFQKLLYNLTSIKIRGTYSERSAGYLDDVTLVTARRGPGTPALWVERCTCPQGYQGQFCEACAPGYRRSRPSLGPFSSCEPCSCNGHSETCDPETGVCDCRDNTAGPNCERCEDGYYGDSSSGTTSDCKPCPCPAGSTCAVVPVTKEVVCTNCPSGTTGKRCELCDDGYFGDPLGQNGAVRSCNPCQCNDNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGFFGNPLAANPADKCKPCSCSPYGTVDRQTTCSPVTGQCQCLPHVINRDCSACEPGYYNLLSGQGCKRCDCNPVGSTNGQCDINTGQCECHPGVTGRQCERCEVNFFGFGSDGCKPCDCDPYGSRTAQCMEDGRCECREGFVGMHCEMCEENYFRNSSRPGCQECPSCYSLVKDKVNQQRRKLQELETLIENLGSSQEAVSDKAFEDRLKEAEKAIMDLLQEAQASKDVDQGLLDRLGDINNTLSTQWNRLQNIRKTVDNTGELADKSRTRVRDTEKLIETARQELEKAKDAINKVDIKPPTSTGDPNNMTLLAEEARKLAEKHKMDADQIEKIAKEANDTSTEAYNLLRKTLDSENKINRDIDELNRKYNEAKDLAKNLEKQANKVHAEAEEAGNKALQIYANLTSLPPVDTKTLEDEANKIKKEASDLDKLIDKTEKEYNDLRDDLKGKEVEVRKLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRGTYQEAQDILNNLKDFDRRVNDNKTAAEDAMKKIPTINATILAANEKTKQAEGALGNAAADAREAKNKAEAAQNIATEVQKNAAKTRADAEKTFEETMKVDNEVNDMMTQLADAEAELAKKKAEADQDMMMANMASNAAQEAEDNARKAKNAVKMVLTTINDLLGQLGNITNVDLSKLTDIDNSLKKAKNDMKDSDLDRKLRELNEAAKSQEDMISDYDRQIQEIKADIANLNDIKNTLPAGCFNTPSIERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3P1</accession>
    <name>W5M3P1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005648">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3P1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002999"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003005.1">
        <property type="protein sequence ID" value="ENSLOCP00000002999.1"/>
        <property type="gene ID" value="ENSLOCG00000002558.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG22"/>
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    <dbReference type="Bgee" id="ENSLOCG00000002558">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF67">
        <property type="entry name" value="PROTEIN SPINSTER HOMOLOG 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="17154" checksum="3307E7806B7B5F0B" modified="2014-04-16" version="1">MGRTHTVEACTNTGVCNLIKTVTSVPFSRSFVWSSLGCTAMAFVTGALAFWTPVFLSRAQVVQKVRQPCNQEPCDTTDSYIFGAVTIVTGIVGVFIGTSIARRLRDSIPHADPLICAVGMLGSSPCFFLAIVLAHRSIPATYVRTLLFLKSQFEVLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4H1</accession>
    <name>W5M4H1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radial spoke head 14 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038890">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038891">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038892">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01038893">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01038894">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038895">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01038896">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AHAT01038897">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5M4H1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003279"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003286.1">
        <property type="protein sequence ID" value="ENSLOCP00000003279.1"/>
        <property type="gene ID" value="ENSLOCG00000002788.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044989"/>
    <dbReference type="HOGENOM" id="CLU_057538_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4H1"/>
    <dbReference type="OMA" id="VWQHDVI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002788">
        <property type="expression patterns" value="Expressed in testis and 7 other cell types or tissues"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
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        <property type="entry name" value="RSP14"/>
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        <property type="entry name" value="RADIAL SPOKE HEAD 14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15599">
        <property type="entry name" value="RTDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="269"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="40504" checksum="3BB5AD2D4BD3BD4B" modified="2014-04-16" version="1">MATARISARLPPNIDPTRAPVAFGHRAVPKLVSALRDSDLLTRQRALMALCDLVHDPETAYQAIETGCLESLKALLKDEDSTVRLKTTEVLYLLATHNVGREALLRGDVLSPLSDLLEEPVAACRRNTHMALEMLAEFPAALLEVVAAELHAEDNGCHAFEAVNPLLALRALAAHVHHLEGALSVVDAGLVPRLVLKLQEECEEVQELILDTLTACLRVDALAALASDAVLALRDCLAHPSVGIRQRAARAMIGLSVPLEGKVRVCEEGVIPVLVGLLSDSHPDVSASAAGSLMNAAITTQGKYLALQAGAITSLLALVSSPRTDVCANALRALTALAEAPPARQELLGHVELLETRRHDTNEIISRAAATAIRVITWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5Y2</accession>
    <name>W5M5Y2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sterile alpha motif domain containing 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5Y2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003790"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003797.1">
        <property type="protein sequence ID" value="ENSLOCP00000003790.1"/>
        <property type="gene ID" value="ENSLOCG00000003206.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXXU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008161"/>
    <dbReference type="HOGENOM" id="CLU_105476_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5Y2"/>
    <dbReference type="OMA" id="RQRVPHK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003206">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09510">
        <property type="entry name" value="SAM_aveugle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039144">
        <property type="entry name" value="Aveugle-like_SAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052268">
        <property type="entry name" value="SAM_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20843">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20843:SF2">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="77"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="164" mass="18738" checksum="3DEF509A35D32652" modified="2014-04-16" version="1">MAVEAVHCNLSQNGIDHAICAEDVSVHLEAESRENGVRNEDSSLRLLEKKGSPKQQTSETDGARPGNVKLSKPVALWTQQDVCKWLKKHCPNQYQIYSNSFKQHDITGRALMRLTDKKLERMGILQESQRQYILQQVLHLRVREEVRNLQLLTQGTAILTVFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6G7</accession>
    <name>W5M6G7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NDRG family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006641181.3"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000003975"/>
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        <property type="protein sequence ID" value="ENSLOCP00000003975.1"/>
        <property type="gene ID" value="ENSLOCG00000003358.1"/>
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    <dbReference type="KEGG" id="loc:102693654"/>
    <dbReference type="CTD" id="65009"/>
    <dbReference type="eggNOG" id="KOG2931">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_035361_1_0_1"/>
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        <property type="component" value="Linkage group LG23"/>
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        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="392"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="432" mass="47707" checksum="348A6B8F93DB09B6" modified="2014-04-16" version="1">MAGLKELQFTEDKPLLPGQDTEMLERREGLRTSAQPRSSKPEAKPPTGLQRSHVIARHWLRITRRRTRGRVPENCETFLSIGDADWKEHDIETPYGMLHVVIRGAPKGNRPTILTYHDVGLNHKLCFNTFFNFEDMQEITKHFVVCHVDAPGQQTGASQFPQGYQYPTMDQLAGMLPSVIQHFGFKSIVGIGVGAGAYILAKFALIFPDLVEGLVLLNIDPNGKGWIDWAATKLSGLTSSLPDTVLSHLFSQEELMSNTELVQSYRQQIGSSINQFNLQLFWNMYNSRRDLEMNRPGTVVNAKTLQCPAMLVVGDNAPAEEGVVECNSKLDPTNTTFLKMADSGGLPQVTQPGKLTEAFKYFLQGMGYIAYLKDRRLSGGAVPSASMTRLARSRTASLTSASSVDGSRARPCTHSDSSDGVGQINHTMEVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7H3</accession>
    <name>W5M7H3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">EGF-like-domain, multiple 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5M7H3">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004331"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004339.1">
        <property type="protein sequence ID" value="ENSLOCP00000004331.1"/>
        <property type="gene ID" value="ENSLOCG00000003643.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="W5M7H3"/>
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        <property type="component" value="Linkage group LG21"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Pro-epidermal growth factor"/>
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        <property type="entry name" value="Dev_Signal_Modulators"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
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        <property type="entry name" value="EMI_domain"/>
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        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF56">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865804" evidence="8">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
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        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="298" mass="33448" checksum="A6E5CFEFA046C981" modified="2014-04-16" version="1">MHPVVLLLTFTLLILPVSCTYHFIQPGRRVCSRDVRQQLTPVSHTESFVQAVHKPYMTLCDGKRLCSTYKTVYRVSYRQVTSPEPPSYSECCPGWRRVHSHNCNQAVCMKPCMNGGTCVRPNQCACAAGWTGRYCQTDIDECNGSHSCAQQCINIVGSYQCGCPEGYQLDRDGHSCEALELSSPNKVNNIPKLSSTTAAIACQNHLEDCFSDEEAGHSNKMTEEVQNLRTRVEMLEQKLQLALAPFNSLFSLMPEDGVAERASFLSHSFQQLDRIDSLSEQIGFLEERLETCSCRDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7I1</accession>
    <name>W5M7I1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Wee1-like protein kinase</fullName>
            <ecNumber evidence="14">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01008206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7I1"/>
    <dbReference type="FunCoup" id="W5M7I1">
        <property type="interactions" value="1500"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004339"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004347.1">
        <property type="protein sequence ID" value="ENSLOCP00000004339.1"/>
        <property type="gene ID" value="ENSLOCG00000003652.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157939"/>
    <dbReference type="HOGENOM" id="CLU_000288_25_1_1"/>
    <dbReference type="InParanoid" id="W5M7I1"/>
    <dbReference type="OMA" id="TIFNHPV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003652">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000115">
        <property type="entry name" value="Wee1-like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000217">
        <property type="entry name" value="Wee1-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017164">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF72">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037281">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="272"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="548"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="278"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="619" mass="68690" checksum="57320F5CBA32D245" modified="2014-04-16" version="1">CAVQGEMSLRAASPKMGPIRQKLQFSPSDGEDDPVEEDGNSTGADSAFQELDSPVQPRQRAAKKRSEEPGDSPRRLHGEEEEDFWDEEGLGSPSPVKSPGSFLMESPSPRKSSSACRGSSPGGAYGREEREGSSSPVPDCPDTPPHKTFRKLRLFDTPHTPKSLLSKASALGSASVRRRGALFRTAEPPSKGASGGKSQQPPHININPFTPDSLLIQSAALQKNSRKRTHWNDSCGEDMEASDGEFEDELFPPSKRITITESNMKSRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLSDVLMENYRSMRYFTEMDLKDLLLQVARGLKYIHSTALVHMDIKPSNIFISRKTVPCVDDCDDDDGLSTSVVYKIGDLGHVTRISNPQVEEGDSRFLANEVLQEDYRNLTKADIFALALTVISAAGAEPMPSNGDKWHEIRQGKLPRMPQVLSLEFLELLKLMIHPDPERRPSASALIKHPVLLTAARMSADQLRVELNAEKFKNALLQKELKKAQMAKAAAEERVLFTDRMMTRSTAQTTSNRTSRLIGKKMNRSVSLTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7K4</accession>
    <name>W5M7K4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger matrin-type protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">U11/U12 small nuclear ribonucleoprotein 20 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640251.1">
        <property type="nucleotide sequence ID" value="XM_006640188.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7K4"/>
    <dbReference type="FunCoup" id="W5M7K4">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004362"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004370.1">
        <property type="protein sequence ID" value="ENSLOCP00000004362.1"/>
        <property type="gene ID" value="ENSLOCG00000003675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683348"/>
    <dbReference type="KEGG" id="loc:102683348"/>
    <dbReference type="CTD" id="55954"/>
    <dbReference type="eggNOG" id="KOG3454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009869"/>
    <dbReference type="HOGENOM" id="CLU_100385_1_0_1"/>
    <dbReference type="InParanoid" id="W5M7K4"/>
    <dbReference type="OMA" id="HNCRFSH"/>
    <dbReference type="OrthoDB" id="2417221at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003675">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000741">
        <property type="entry name" value="Zinc finger matrin-type protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465">
        <property type="entry name" value="NUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465:SF0">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="174" mass="19996" checksum="2E377F9B9A70C461" modified="2014-04-16" version="1">MGKRYYCDYCDRSFQDNMHNRKKHLNGVQHQRSKKAWFDNFRDASAILADEQTKKPCRKFLQTGQCVFGSNCRFSHMTDRDIGSLKHQIEEERRLKDDPDLGRKLSLGSIEDWLAQREKRHRAPESTSVLKAEGADSSLGSDLPPHFLSIPDLPVSLMPPPSGGWMKVPHTEWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7V5</accession>
    <name>W5M7V5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Reticulon 4 receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Nogo receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221509.1">
        <property type="nucleotide sequence ID" value="XM_015366023.2"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000004463"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004471.1">
        <property type="protein sequence ID" value="ENSLOCP00000004463.1"/>
        <property type="gene ID" value="ENSLOCG00000003749.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691368"/>
    <dbReference type="KEGG" id="loc:102691368"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160711"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_6_1"/>
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    <dbReference type="OMA" id="MWLNLLP"/>
    <dbReference type="OrthoDB" id="546383at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003749">
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    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ganglioside GM1 binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:ganglioside GT1b binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF174">
        <property type="entry name" value="RETICULON-4 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865831" evidence="17">
        <location>
            <begin position="27"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="322"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="479" mass="54010" checksum="927C4BFBA9277469" modified="2014-04-16" version="1">MKTTVAEGSRLLFLVLWLNSVPQVECCPGVCVCYSEPRTTVACQQQGLSSIPTEIPIRSQRIFLQNNKLTVVKSTSFSSCHNLTVLWLYSNNISHIEAGAFYGLERLEELDIGDNSNLRIISPTAFRGLSRLHTLHLHRCGLSELPIGVFRGLFSLQYLYLQDNNLLTLHDDTFLDLANLTYLFLHGNKIKTVSENMLRGLIGLDRLLLHQNRVTSVHRRAFHDLGKLTTFYLFNNNLTVLTGETMDYLVSLQYLRLNGNQWICDCRARSLWDWFKRFKGSSSELECHVPASLVGKDLKRLKASDLEGCLDNSHQIRTSIFSTKTRSGKLPSTETPLREGSPRCCLPESDKSSIISSKGLPDPSSYNSRQITNNPLKEKENISKPKFPDVDPTKNGTRNKQRLNDDPVGTFSNTQDDPLGKLKPDYTDNLEPSTAPSRKKKKCSKKPKPDAQCLKSKGSTSRALNLLFLPIIWFSLTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9M2</accession>
    <name>W5M9M2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 8 open reading frame 34</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5M9M2">
        <property type="interactions" value="330"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000005089.1">
        <property type="protein sequence ID" value="ENSLOCP00000005081.1"/>
        <property type="gene ID" value="ENSLOCG00000004246.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUHG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005870"/>
    <dbReference type="HOGENOM" id="CLU_030572_1_0_1"/>
    <dbReference type="InParanoid" id="W5M9M2"/>
    <dbReference type="OMA" id="DPRVYNS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004246">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040687">
        <property type="entry name" value="DUF5586"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32000:SF3">
        <property type="entry name" value="RIKEN CDNA A830018L16 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32000">
        <property type="entry name" value="SIMILAR TO HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17824">
        <property type="entry name" value="DUF5586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="614" mass="68279" checksum="93B52AE801562F7F" modified="2014-04-16" version="1">EMMTKLITETPDHPIPFLINHLQTKQGSPSKLQRTLSGSAALWADTGTTENKGTRRDFRSYEKPWQIHPKKPKKSKSDLAVSNISPPSPESKSLPRSIEHPSWDWRTRPESHDFDELNHILQESKKLGKALENLSRSIAVSDELDQDPLAFNCSLLRPRVIGEWVGREEHDADPLAAEMLQPPVPRTKTEQWESEDSSGPAGRIKWLKKKKGRAQGRDHHKKLIKKMLQKTSFYSTHPKNPSVTVEFVNYDQDCWLLTEDLDDLRMEGVTSLAPSGSKFSQGRSSYCPEPQARVTLNICSRCARLQGDSLLEARDEDLHTAQSPEQAVPEIVCPLPGAETLMEEDEEFESASQVTGPRQPVWDSELMSARGGTSPSQKQLRESFFSKELLCMEKHLAEVEKDIAKMAESTLLRSPNSPHSSLLLTPVLQGGLPSLPGPNSRPQSPGGFAGKSSPLSLQTNKPVSLATSRPQTPSNHNSRPQTPNSQSSRPRTPSGNRPVTPNSLMPRPVTPSNQGNKNENFGSQRSRPVTPTSQSGRPLLTPTGLTAGAITPENMGKAQSQRTNLPEDEFYQQLQAVRQPWLIPSDTDSDCVDQPDQDKCEFRPRSTIYSLNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA64</accession>
    <name>W5MA64_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Haptoglobin-related protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA64"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005273"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005281.1">
        <property type="protein sequence ID" value="ENSLOCP00000005273.1"/>
        <property type="gene ID" value="ENSLOCG00000004416.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159903"/>
    <dbReference type="InParanoid" id="W5MA64"/>
    <dbReference type="OMA" id="YMKISSY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004416">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000241">
        <property type="entry name" value="Haptoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="27"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="296" mass="33059" checksum="893D0CE7EF1D6087" modified="2014-04-16" version="1">MVLGLCLADKPDIQEQRHQALFRSRRMVGGVLARRVPWQTLVTLGDKIIGGGTLIGKRWVLTAGRNLFTNASRNATLYQAPAIPKVYLSITDLREREETFNEVKVDQVFLHPNFQNTSDWENDLALIRLKEDLFLDGNVKPLSLPEKDYALMGTQGDVSGWGRNALLQYSRLLKTLTLTVANHTMCKETYSSGGQVVSSTPIVDDNMFCTEATSYREDVCIGDAGGAFAVQDPKDGKVYVAGVLSFDKSCAVERYAVFMKISAYVPWIKILIWGKEKPIQTSVIFIAFGNPYHKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA73</accession>
    <name>W5MA73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">trypsin</fullName>
            <ecNumber evidence="6">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01019331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA73"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005282"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005290.1">
        <property type="protein sequence ID" value="ENSLOCP00000005282.1"/>
        <property type="gene ID" value="ENSLOCG00000004422.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244883"/>
    <dbReference type="HOGENOM" id="CLU_006842_1_7_1"/>
    <dbReference type="InParanoid" id="W5MA73"/>
    <dbReference type="OMA" id="RYCARED"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004422">
        <property type="expression patterns" value="Expressed in liver and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000166">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF7">
        <property type="entry name" value="TRYPSIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865923" description="trypsin" evidence="7">
        <location>
            <begin position="18"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="26"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="186" mass="20465" checksum="70BDDF7E1D9DE718" modified="2014-04-16" version="1">CARSLVTVALCSVPVASAPGGDDDKIIGGYECRPHSQPWQVYLTYDNGQRWCGGSLINEWWVVSAAHCYIPPPRLEVHLGEHHLFRDEGSEQHIPAAKVIRHPGYNERTTDNDFMLIKLRQAARLNQYVQPIALATSCVTAGTPCLVSGWGNQLTNDVNYASVLQCLNLPVLSDGQCRSSYGSQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA95</accession>
    <name>W5MA95_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibitor of nuclear factor kappa-B kinase subunit alpha</fullName>
            <ecNumber evidence="3">2.7.11.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear factor NF-kappa-B inhibitor kinase alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <text>L-seryl-[I-kappa-B protein] + ATP = O-phospho-L-seryl-[I-kappa-B protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
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    <comment type="subcellular location">
        <subcellularLocation>
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        <subcellularLocation>
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</entry>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <sequence length="378" mass="44716" checksum="D19EC00B54E6E432" modified="2014-04-16" version="1">NYNAETSSPFSLQESADAIISVHMLECKTSLLEAVEELRIRRDAEIRFEDQINKLVLEKQELEWEKESLQNQTDTLKSQQTDALTALKKKLKARIRGIEGEKGKHQLTTELRDKEINSLKEELKSLQVFKFSLQKKLSELEQKLQLQTQTKDNHLNQISEAEKRFETISRQCVMVKQFHEKLEQNVEEAMRLNKKLTLLNYNQEATIFRLKKDIEKLNKELIKSRVSSHCKRGDESMHRTLQEQEIQQLQHKLLVETEMTKRLTKENATIREEKQEVMNSLKHAQQLLQRQTEAIGRVELELNAQREEHQSDEKENSTSLKITGEEDKFISLTEECQNSKAIWEKEQMRLQEKIQQTQEKLRSHKEAYDYLHEQHNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAJ0</accession>
    <name>W5MAJ0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAJ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005399"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005407.1">
        <property type="protein sequence ID" value="ENSLOCP00000005399.1"/>
        <property type="gene ID" value="ENSLOCG00000004489.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002461"/>
    <dbReference type="HOGENOM" id="CLU_005034_2_1_1"/>
    <dbReference type="InParanoid" id="W5MAJ0"/>
    <dbReference type="OMA" id="WSIMPLN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004489">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000856">
        <property type="entry name" value="ER membrane protein complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867534" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="22"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="14">
        <location>
            <begin position="21"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="787"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="993" mass="111818" checksum="8402A997306A2424" modified="2014-04-16" version="1">TMAGFLAGFAFFSFLLYHSAAVFEDQVGKFDWRQQYVGRVRFAYFDTQTQASKKLLLATEQNIIASLNSRNGEIFWRHVDKSGPEGGIETMLFHGQDAVVVSGNGRLLRSWETNVGGLNWETLLDTGSFQAATFVGLQDSVKYVAVLKTGAISLHYLSNGHQRWVENLPDSETVQYQTVYSGGSGEVYVLGVVPHSHLVIIAYNIEDGEIMKQRSVEAPWLASLQPGCAVVSTGVLVCVDPTTQSLYTLPLDTDHQQDMKQLLLQSLDLEVSLGFQPVIVSTQPNPARPPLPEFFLQLGPDHHTLLQLGDGLVISLRAFQPTKSAPLIFPTVGIAFIAVRLFCLNRTEAPPISARLSKKDVKMRLASAQLCALGLLEPVRHFIDDCLTSLSLTVFLYSSHCYVWIQLFKVKCTSLCFLYIIVQIDRLKVRYLKHSEARRVMWILSLKLCKERGSVYRSADGPQRLSGSCFSPDGLLGMVLKRLSSQLILLQAWTAHLWKLFYDARKPRNHVKNEVTIETLARDEFNLQKMMVMVTASGKLFRGFRSFSSLKWGIAVETNMVKVKYKLKTTQKKSVSKHKYERSTNIDPQFSGLSSHPVGVPCFGVTSSQVGEGSPGVAVPVRYKITYMIQLLISDHAYICLMVDPFIQVTAFPSTKNVLQQLQEMASSIFFYLVDSAQGRLAGFRLRKDLSTELIWEVVIPTEVQRIVAVKGKRANEHVHSQGRVMGDRSVLFKYLNPNLLAVVTESTDAHQERSFVGIYLIDGVTGRIIHEAVQRKARGPVHFVHSENWVVYEYWNTKLRRNEFSVLELFEGTEQYNSSAFSSLDRPHAPQVLQQSYIFPSTISTLEATLTEKGITSRHLLVGLPSGAILSLPKMFLDPRRPEVPSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB15</accession>
    <name>W5MB15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DnaJ homolog subfamily C member 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CSL-type zinc finger-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Diphthamide biosynthesis protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer and homooligomer. Iron binding promotes oligomerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008221">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB15"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005574"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005582.1">
        <property type="protein sequence ID" value="ENSLOCP00000005574.1"/>
        <property type="gene ID" value="ENSLOCG00000004654.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005430"/>
    <dbReference type="HOGENOM" id="CLU_017633_7_3_1"/>
    <dbReference type="InParanoid" id="W5MB15"/>
    <dbReference type="OMA" id="LEDMTWE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004654">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000056">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000002">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45255">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45255:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DPH-type MB" evidence="16">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="152" mass="17559" checksum="68D66FB16EEEADE6" modified="2014-04-16" version="1">TDMTDESALQKDWYAVLEASPSDSLQELKQKYQKLALLYHPDKQSPDVPPGEVEQCVQRFIEVDQAWKILGNEETKKNYDLQRHAAELKQGWPIDAYVRLEDMTWQDDEQAYLYGCRCGGEYMMSKEEAEENVSVICCDTCSLSIEVKLHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBI8</accession>
    <name>W5MBI8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lymphotoxin-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">TNF-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Tumor necrosis factor ligand superfamily member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits. Interacts with TNFRSF14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBI8"/>
    <dbReference type="FunCoup" id="W5MBI8">
        <property type="interactions" value="422"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005747"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005755.1">
        <property type="protein sequence ID" value="ENSLOCP00000005747.1"/>
        <property type="gene ID" value="ENSLOCG00000004793.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S4K8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248544"/>
    <dbReference type="HOGENOM" id="CLU_070352_4_0_1"/>
    <dbReference type="InParanoid" id="W5MBI8"/>
    <dbReference type="OMA" id="ENILPWE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004793">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006053">
        <property type="entry name" value="TNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002960">
        <property type="entry name" value="TNF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF24">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01234">
        <property type="entry name" value="TNECROSISFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01236">
        <property type="entry name" value="TNFBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="THD" evidence="11">
        <location>
            <begin position="33"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="20134" checksum="24A24C8CF099BBB5" modified="2014-04-16" version="1">QSQVCVFTVCKNLQKGPSQQMALLTPSPKRSMPKAHLTAAKMETTAKLENILPWESNLGNAFTDSGFAYTDNALLIPESGRYFVYAQVTFRALMHDHGKRGCEPVRLSQTISKMTNGYPTPSDLITSFKTVECGSDTWMKSLYSGAVFLLEKGDKLMVLVNNITLVDFTDEKKTFFGAYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBJ0</accession>
    <name>W5MBJ0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01021989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBJ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005749"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005757.1">
        <property type="protein sequence ID" value="ENSLOCP00000005749.1"/>
        <property type="gene ID" value="ENSLOCG00000004794.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686971"/>
    <dbReference type="KEGG" id="loc:102686971"/>
    <dbReference type="CTD" id="85443"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159476"/>
    <dbReference type="HOGENOM" id="CLU_000288_94_2_1"/>
    <dbReference type="InParanoid" id="W5MBJ0"/>
    <dbReference type="OMA" id="VRAQKKW"/>
    <dbReference type="OrthoDB" id="1738954at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004794">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16111">
        <property type="entry name" value="DCX_DCLK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14095">
        <property type="entry name" value="STKc_DCKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000315">
        <property type="entry name" value="Calcium-dependent protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000066">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000013">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Doublecortin" evidence="13">
        <location>
            <begin position="88"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="500"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="310"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="436"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="793" mass="91430" checksum="D3394BA162E3E18F" modified="2014-04-16" version="1">MPAFKEQGPSEVASPTWRQSGQQASLPISIKDLRQANCDIRLQATYPPMSSSTFGKTLSGTTCFKNPFHHNGNGFHTIHAENSPLKPRIVTVVRPGSKRNLRKITILLNRRSVQTFEQLLADISEALGFPRWKNDRVKRLFSLKGREIRSVSDFFRGDDVFIAAGREKLTAKDFQDVLEELYPECPYSQSIVQQNWEKFQTPQTKTSKADSGFHEEPIVTKQKGPKLPKQAATNLQRVRDKARQEERLKARRWERERWERQQKELSEKNRFKGRLPHRDEDIENFAHIDLENASLCEKCKRNRGRREQCTLIQRKNVSPDKKAQGREEDQPEKLPLEKRRQKPKSSKMSEDNFQKEKHECEDTKKYRKESSWTCKEHGDMSIFHGQSKSEKTTELEDKKCAVEKQSARNPCDSEVRNENSVSERFLNGRPNFTENKSPDKAQTEKVSEDFQQKNVHKEGQREAETRKSQEEVQSSERESSRNCILKPRLVKTKSDIESYYEIGRAIGDGNFAVVKECKLRNTDFAYAMKIISKAKLKGKEHMIENEISIIKSLSHPNVVRLLQDYETESDIYLIMEIVQGGDLFDSITESVKFTEHNASLMVNDLCQALAYIHSKNIVHRDLKPENLLVQHNKDGSTTLKLADFGLAVVVTEPIFTVCGTPTYVAPEILSEKGYGLPVDMWATGVIVYILLCGFPPFRSLERDQEELFEIIQLGEYEFLSPYWDNISDGAKDLISKLLLVDPEKRYAAQQALQHPWVQSGSKNSFNLQREVTTNIERHFKNRRKREHASAADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBU5</accession>
    <name>W5MBU5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RNF166</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RING finger protein 166</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RING-type E3 ubiquitin transferase RNF166</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01010517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBU5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005862.1">
        <property type="protein sequence ID" value="ENSLOCP00000005854.1"/>
        <property type="gene ID" value="ENSLOCG00000004854.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102683008"/>
    <dbReference type="GeneTree" id="ENSGT00950000182909"/>
    <dbReference type="HOGENOM" id="CLU_092448_1_0_1"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004854">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16549">
        <property type="entry name" value="RING-HC_RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034734">
        <property type="entry name" value="ZF_C2HC_RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18574">
        <property type="entry name" value="zf_C2HC_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51803">
        <property type="entry name" value="ZF_C2HC_RNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="41"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC RNF-type" evidence="17">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="245" mass="27288" checksum="C54F8DD5EFD2ABDD" modified="2014-04-16" version="1">MMAMFRNFVSATQHRQHPQQQQQSGAAAAAPGDSLETQFSCPICLEVYHRPVSIASCGHTFCGECLQPCLQVPSPLCPLCRMPFDPKKVDKSSNVEKQLSSYKAPCRGCSKKVTLVKMRSHVSSCTKVQEQMANCPRFVPVVPTSQPIPSNIPNRSTFVCPFCGARNLDQQELVKHCMENHRNDPNKVVCPVCSAMPWGDPSYKSANFLQHLLHRHKFSYDTFVDYSIDEEAALRAALALSLSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC13</accession>
    <name>W5MC13_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC13"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005922"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005930.1">
        <property type="protein sequence ID" value="ENSLOCP00000005922.1"/>
        <property type="gene ID" value="ENSLOCG00000004926.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000153"/>
    <dbReference type="HOGENOM" id="CLU_107248_1_0_1"/>
    <dbReference type="InParanoid" id="W5MC13"/>
    <dbReference type="OMA" id="IRVKKYM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004926">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060215">
        <property type="term" value="P:primitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="137" mass="15756" checksum="20F9F3262ACE1113" modified="2014-04-16" version="1">QNDTVTIRTRKFMTNRLLQRKQMVVDVLHPGKATVPKTEIREKLAKMYKTTPDVVFVFGFRTQFGGGKTTGFAMVYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAAGKKVMPTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC74</accession>
    <name>W5MC74_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin subunit alpha 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC74"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005983"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005991.1">
        <property type="protein sequence ID" value="ENSLOCP00000005983.1"/>
        <property type="gene ID" value="ENSLOCG00000004959.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159891"/>
    <dbReference type="HOGENOM" id="CLU_004111_1_0_1"/>
    <dbReference type="InParanoid" id="W5MC74"/>
    <dbReference type="OMA" id="HTYELIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004959">
        <property type="expression patterns" value="Expressed in muscle tissue and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF90">
        <property type="entry name" value="INTEGRIN ALPHA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1014"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="279"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="341"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="400"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="444"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="609"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="778"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="877"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1066"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="877"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1088" mass="120315" checksum="17F6B20A7510DC28" modified="2014-04-16" version="1">SRILVGAPQARGFQPQHDVRTGALYQCPITAEEWDCVRVDIDKGVSPDHESKANQWLGVTVKSQGIGGKVVTCAHLYELRQWVRRQQETRDPIGRCYMLSEDLTEQDDLDGGEWKFCEGRPQGHEQFGYCQQGMSASFTPDRNYILFGAPGTYNWKGELRVQLLNQSMLYLDFFDDGPYEVADEKKQDESLIPVPYNSYLGLLFMTSPVEETLVYKTLEPSGRATTFEDVAQNSYLGFSVDSSKGITSKSELTFVAGAPRANHTGAVVLLLKDNVYRLVPMHILWGEELASSFGYSVAVADLNSDGWVDLIVGAPNYFDRKAEIGGAVYVYLNPAGRWEWARPTRLNGTYNSMFGLTVSSVGDLDQDGYGDIAVGAPFDGDGKVFIYRGSSSGIITSPAQVLDGVGVGVRRFGYSISGGLDIDGNSYPDLAVGSLGDQVVLYRSRPVIHVARQVTIKPQNIDLEQSNCKGRDGVCVVVEACFEYRAHPRTYSPHITLGVLFEADTERRKLVLPHRVNFLERTGAEPEYQHSVEVDLRGQLRTSCQMATFQLQDTLRDKLRPISISVTHSIKRARLSNVPSGQLGPLLPVLSTAMSNTLQTEVNFLREGCGEDKICQSNLQLSYRFGTRPAPADPFTPLPRDDDGMQVFSLSNQKAVALEVKVTNLPSDPRRPQDDGDDAHAAQLLVALPDTLSYSGFRGPQDKQLVCQANQNGSQAECELGNPLKRGAELTFYLILSTSGITIETTDLSVTLQLATISEQPDLKPMKARAKVVIELPLSVTGVARPHQLFFSGAVRGESAMTSLEDVGSPVEYEFTVTNPGQSLQTLGSAFLNVMWPHELANGKWLLYPTSMQFKGHPNTRCTASEPLDVLGLNRNSHSQAAQHTSSHSRKGRSHPEEQDRILTQSILGRTTPAVAASERKKSLTLDCLLGSARCMLIQCPLHSFAGSATLKVQARLWNSTFLEEFPSVSDLELLVRANITVKSTIKHLMLRDAASEIPLRIYPEHGISDQYGIPWWIILIAVLAGILVLALLVCLLWKCGFFRRAQYKDTVPQYHAVKIPREDRPQFKDGKTGTLQKKEWTTHWSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME42</accession>
    <name>W5ME42_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME42"/>
    <dbReference type="FunCoup" id="W5ME42">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006651"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006659.1">
        <property type="protein sequence ID" value="ENSLOCP00000006651.1"/>
        <property type="gene ID" value="ENSLOCG00000005506.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00390000016051"/>
    <dbReference type="HOGENOM" id="CLU_047858_1_0_1"/>
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        <property type="component" value="Linkage group LG17"/>
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        <property type="term" value="C:transcription factor TFIIF complex"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="General transcription factor IIF subunit 2"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="TFIIF_beta"/>
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        <property type="entry name" value="TFIIF_beta_HTH"/>
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        <property type="entry name" value="TFIIF_beta_N"/>
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    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="16"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="201"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="274" mass="30864" checksum="A1A71F20A4318623" modified="2014-04-16" version="1">MSEKGEVDLTGAKQNTGVWLVKVPKYLSQQWAKATGRGEVGKLKICKKRNQGKAEVSFTLNEELATITDIGDKSSTVKAPRDHPFTMQSVGGQTLAVFTESSSGQSEEKSDSSSPDLSGIKTGTEEDKIALEGVVVQRAECRPAVNENYMKLKKLQIEESSKPARLSQQLDKAVTTNYKPVANHTYNVEYEKKKKEEGKRARADKQQVLDMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILRGIGIYNVKGTHKNTWELKPEYRHYQGEDKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEJ4</accession>
    <name>W5MEJ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nitric oxide synthase</fullName>
            <ecNumber evidence="17">1.14.13.39</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Produces nitric oxide (NO) which is a messenger molecule with diverse functions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2O</text>
            <dbReference type="Rhea" id="RHEA:19897"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19898"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin</name>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="17">Binds 1 FAD.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="17">Binds 1 FMN.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the NOS family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.39" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01005815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEJ4"/>
    <dbReference type="FunCoup" id="W5MEJ4">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006803"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006811.1">
        <property type="protein sequence ID" value="ENSLOCP00000006803.1"/>
        <property type="gene ID" value="ENSLOCG00000005624.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692649"/>
    <dbReference type="KEGG" id="loc:102692649"/>
    <dbReference type="CTD" id="563654"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159752"/>
    <dbReference type="HOGENOM" id="CLU_001570_16_0_1"/>
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    <dbReference type="OMA" id="CRHIRYA"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005624">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004517">
        <property type="term" value="F:nitric-oxide synthase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045776">
        <property type="term" value="P:negative regulation of blood pressure"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007263">
        <property type="term" value="P:nitric oxide mediated signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032496">
        <property type="term" value="P:response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00795">
        <property type="entry name" value="NOS_oxygenase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nitric oxide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000039">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nitric oxide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.440.10:FF:000005">
        <property type="entry name" value="Nitric oxide synthase, inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.340.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1230.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.440.10">
        <property type="entry name" value="Nitric Oxide Synthase,Heme Domain,Chain A domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050607">
        <property type="entry name" value="NOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044943">
        <property type="entry name" value="NOS_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044940">
        <property type="entry name" value="NOS_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044944">
        <property type="entry name" value="NOS_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012144">
        <property type="entry name" value="NOS_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004030">
        <property type="entry name" value="NOS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036119">
        <property type="entry name" value="NOS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410:SF4">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02898">
        <property type="entry name" value="NO_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000333">
        <property type="entry name" value="NOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56512">
        <property type="entry name" value="Nitric oxide (NO) synthase oxygenase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60001">
        <property type="entry name" value="NOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 17" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 17" id="KW-0288">FMN</keyword>
    <keyword evidence="6 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="15 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="9 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0521">NADP</keyword>
    <keyword evidence="14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="19">
        <location>
            <begin position="545"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="736"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000333-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1145" mass="130288" checksum="4C95903BF434F068" modified="2014-04-16" version="1">MLLSWNYFQKLKNFKNSSRSIDLNNNNSFDAGAKYSKHFEDDLQKIEEEKKAQIVPEIQVEKVNNVMPAPSQMPPRSCAQNPARSRCPFFIKVKNIEDSSSLQDTLHHRSAKHLPCKPSVCQGSIMNPKCLSRGPRDKPVPADEILVQAIEFVNQYYQSFKQPRTEDHLARVEAVTMEIEKTGTYQLTFDELAFAAKQAWRNAPRCIGRIQWANLQVFDARQCTTTKEMFEFLCNHLKYATNEGNLRSAITVFPQRSDGQHDFRVWNGQLVKYAGYQMPDGTVLGDPTSLEFTELCVQLGWKPRYGRFDVLPLVLQANGEDPELFEIPPELILEVPMEHPQYEWFKELDLKWYAMPAVSNMLLEVGGLEFTACPFNGWYMGTEIGVRDFCDANRYNILEDVGRRMGLETHRLSSLWKDQALVAINVAVIHSFQKHNVTIMDHHSAAESFMKHMHNEFRLRGGCPADWVWLVPPMSGSLTPVFHQEMVSYILSPFYYYQPDPMTVHVWKDEQKRPKKRKIYFRDLARAVFFTSSLMRKVMAARVKATVLYATETGKSETFAKKLNTMLNCAFNSRVLCMEDYDFTDLEKETFLVIVTSTFGNGDSPGNGETFKKSLFALKRLKNKLRYCVFGLGSRMYPQFCAFAHAVDKKLADLGAQRLTLTGEGDEMGGQEEAFSSWAGVAFKDACETFDIRGQLNIQLPGSDRLSNTWDPKRYRLANESQTLDLINALSTIHSKDVFAMKLKKRHNLQSLHSSRSTILVELVRHGTQDLRYLPGEHIGVLPGNCAQLVSGVSEHLNDAPPSNQTLRLEVCPSTSKGGAADWETDTRFPACTLSQALTYFLDITAPPSQSLLRKLAQLAAEDSHRERLLTLAQDTKEYNTWKTFNSPTFLEVLEEFPSLQLPTTFLLSQLPLLKPRFYSVSSSVDRHPGEIHLTVAVVRYNTRDGQGPLHHGVCSTWLDTIKEGDVVPCFIRSANGFHLPEDPTAPAILVGPGTGIAPFRSFWQQRLHDLETRGVRAAPMTLVFGCRQSNTDHLYRKETLELKRRGILKDAITAYSRQPGQPKVYVQDVLRDRLSEEVVRVLHQQQGHIYICGDVRMARDVEETLAQILAHKLGMSLEQAVNYLSQLKTEKRYHEDIFGAVFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF14</accession>
    <name>W5MF14_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phospholipase A2</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01005833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF14"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006973"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006981.1">
        <property type="protein sequence ID" value="ENSLOCP00000006973.1"/>
        <property type="gene ID" value="ENSLOCG00000005777.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTYI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165341"/>
    <dbReference type="HOGENOM" id="CLU_817722_0_0_1"/>
    <dbReference type="InParanoid" id="W5MF14"/>
    <dbReference type="OMA" id="KWHTISH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005777">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04704">
        <property type="entry name" value="PLA2_bee_venom_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000002">
        <property type="entry name" value="Phospholipase A2 group III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="11">
        <location>
            <begin position="102"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="315" mass="35084" checksum="EBDE4845B0CA6D42" modified="2014-04-16" version="1">MFNGTFCAKSARVGAHDLHQVPDGVEEVRSVRDPRGGLLDCAVVSSPMEVKSFMHVCRLGLRDRRRASPAPQGQTAGAERGESQDVGGARTAPRRTKRGFTYPGTLWCGAGNNAEHYDHLGEFADTDSCCREHDHCPYVIQPFSSRYGYRNFRWHTICHCDCDNALKACLRKVNDTSSRVVGQAFFNVIQVPCFEFRYEEQCVERLWYGWCQRYEKVPIAVAQESVTYDFGGIEVIDELTIAPPARKDEEEEGARAPATATQAPPALGHMVTAAEDFIKVLATVSTSQSTVAESAKEEREQGAEQKKNKKTSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG81</accession>
    <name>W5MG81_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Cyclic nucleotide gated channel subunit beta 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG81"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007390"/>
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        <property type="protein sequence ID" value="ENSLOCP00000007390.1"/>
        <property type="gene ID" value="ENSLOCG00000006113.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162283"/>
    <dbReference type="HOGENOM" id="CLU_005746_11_0_1"/>
    <dbReference type="InParanoid" id="W5MG81"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006113">
        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="F:cGMP binding"/>
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    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
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        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
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    <dbReference type="GO" id="GO:0098655">
        <property type="term" value="P:monoatomic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0001895">
        <property type="term" value="P:retina homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyclic nucleotide gated channel beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyclic nucleotide-gated channel beta 3"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
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        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF16">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="8" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="834"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="883"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="950"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="1054"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="263"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <end position="593"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="608"/>
            <end position="648"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="668"/>
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            <begin position="1174"/>
            <end position="1202"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="19"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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            <begin position="23"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <sequence length="1220" mass="136965" checksum="7C8D09467F677330" modified="2014-04-16" version="1">MFSWVVKVVPQPPEPPKPLGPQEKVEPKPSAPEPEKKVTEKEKAKEQPPKDAAGKDTENVDPQGKDSTAKTEPEKPKAQEDKSEPPGVLTWISQGLVNVLPKPVESPQKPSPRRARNVPNPPGTTTINLQIPGHLGLGKVVPQPEEKHKAVAPAEGPNVIEWLKQGLEKVVPHPTEQPAPLNTAPQKQAAEEKKEEAPKKETGKKETAGEAPAPAPAPAPAPAPAPAPAPAPEKGFSALPRELLRTLVVSWFVQGLEKMIPQPVTKPKKRISEKENDTPEQCSVEYFSILPDPAGGDLVLEEVESDWENEPTEQQAPPAMPLEETPPLQEASQEPPTHPRILAMQQEDAETQTERWTPLLQAIQQEAEAAARAALEDRLRQEKMATEKTTEELGERAEQSVGELDDGEHFVKTAQYPGGREEQDMGLYSKIEGRQFNQIEGTKLSRLRFEDPSEEMRADSFRKPGPEPEPEPEPEAEPEAGEGGSPAQQPQRVQESPVREVPPAEDLREEAAQEAEPVTQQPDTTEALEPPAGAHQEEESQAVEEECGESSRPSIHPSVNVEDVDSDGDASGGHQKTSPVILPSSPYTSKTLTVPGVPVAARRKKLYSQEESMEDAETTVKAWPSQTSLLSEDEMRERPASAASQTSTIVNERLQELVKMFKERTERVKEKLIDPDESDKDSPSATPEKKLPPPPPPPEKKEEPAPEKEGEEHYCEMLCCKFKTMPWMTRLKEYRFPQSIDPFTNLIYVLWLFFVTAAWNWNVWLIPVRWAFPYQTPDNIHLWLLMDYLCDTIYILDILVFQPRLEFVRGGDIVCDRKDMRENYMKSHRFKMDLIALFPLDLLYLKVGVNSLLRFPRLLKYMAFFEFNDRLEAIMSKAYIYRVIRTTAYLLYSLHCNSCLFYWASDYEGLGSTKWVYDGIGNSYIRCYYFAVKTLITIGGLPDPTTLFEIIFQLINYFVGVFAFSIMIGQMRDVVGAATAGQTYYRACMDNTIKYMTSYRIPRDVQNRVKTWYDYTWQSQGMLDEQELLIQLPDKMRLDIAVDVNYSIVSKVALFQGCDRQMIYDMLKRLKSVVYLPGDYVCKKGEIGREMYIIKAGEVQVLGGPDGKTVFVTLRAGSVFGEISLLAVGGGNRRTANVVAHGFANLFILDKKDLAEILVHYPESQKLLRKKAKKMLTKDKKPTDEKAEQKTGKKAIPLQPETPKLFQAALEVTEKSGIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGL9</accession>
    <name>W5MGL9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KH-type splicing regulatory protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGL9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007528"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007536.1">
        <property type="protein sequence ID" value="ENSLOCP00000007528.1"/>
        <property type="gene ID" value="ENSLOCG00000006229.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156051"/>
    <dbReference type="HOGENOM" id="CLU_014285_1_0_1"/>
    <dbReference type="InParanoid" id="W5MGL9"/>
    <dbReference type="OMA" id="PQGANTI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006229">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22479">
        <property type="entry name" value="KH-I_FUBP2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22482">
        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22485">
        <property type="entry name" value="KH-I_FUBP2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22488">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000008">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000010">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015096">
        <property type="entry name" value="FUBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047372">
        <property type="entry name" value="KH-I_FUBP2_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047369">
        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047370">
        <property type="entry name" value="KH-I_FUBP2_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047371">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09005">
        <property type="entry name" value="FUBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="87"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="174"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="266"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="367"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="338"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="635"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="472"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="663" mass="70153" checksum="D8DFA606EBF8E639" modified="2014-04-16" version="1">MSPRTRFLNEPSRFGSRGCDHGTEIAAKIGGDAGTAPLNNTSSTESYTFTAQKRQLEDAEQPDSKKMASQSEPIGAQLAALHQQRPASTTEEYRVPDGMVGLIIGRGGEQINKIQQDSGCKVQIAPDSGGLPDRSVSLTGTPESIQKAKMLLDEIVSRGRGTPPTFHESTNGQSGSVQEIMIPAGKAGLVIGKGGETIKQLQERAGVKMILIQDGSQSANMDKPLRIIGEPYKVQQARELVQEILRERDHPGFVERNEYGSRMGGGGGGIDVPVPRHSVGVVIGRNGEMIKKIQNDAGVRIQFKPDDGTGPDKIAHIMGPPERCEHAARIIADLLQSIRSGPPGPPGPGMPPGGRGRGRGQGNWGPPGGEVTFSIPSHKCGLVIGRGGENVKAINQQTGAFVEISRQPPPNGDPNFKLFTIRGTPQQIDHAKQLIEEKIEGPLCPVGPGPGGPGPSGPMGPYNPSPYNQGPPGGPPHGGPPPPHQYPPQGWGNTYQQWQPPGPHDPSKAAAAADPSAAWAAYYAQYYQQPPAPVPGQPPAAPTGAPVPTDQPQASQAPAGQPDYTKAWEEYYKKLGKSRRWACGTELPESKGDWPYFSFPSAQAAGTAPPASGAQTDYSAAWAEYYRQQAAYYGQTGGQPAGQTAPPQQGQQVPMASRHHTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHF3</accession>
    <name>W5MHF3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2</fullName>
            <ecNumber evidence="18">2.4.3.2</ecNumber>
            <ecNumber evidence="19">2.4.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Gal-NAc6S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Monosialoganglioside sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">ST3Gal II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">ST3GalA.2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Sialyltransferase 4B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="primary">st3gal8</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="journal article" date="2015" name="Mol. Biol. Evol." volume="32" first="906" last="927">
            <title>Integrative view of alpha2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.</title>
            <authorList>
                <person name="Petit D."/>
                <person name="Teppa E."/>
                <person name="Mir A.M."/>
                <person name="Vicogne D."/>
                <person name="Thisse C."/>
                <person name="Thisse B."/>
                <person name="Filloux C."/>
                <person name="Harduin-Lepers A."/>
            </authorList>
            <dbReference type="PubMed" id="25534026"/>
            <dbReference type="DOI" id="10.1093/molbev/msu395"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:139596"/>
            <dbReference type="EC" id="2.4.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:21617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a ganglioside GA1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27938"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a ganglioside GA1 + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:88069"/>
            <dbReference type="ChEBI" id="CHEBI:90151"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a ganglioside GM1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1a (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77709"/>
            <dbReference type="ChEBI" id="CHEBI:78445"/>
            <dbReference type="EC" id="2.4.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:18022"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a globoside GalGb4Cer + CMP-N-acetyl-beta-neuraminate = a globoside MSGG + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:140623"/>
            <dbReference type="ChEBI" id="CHEBI:140691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:65373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>ganglioside GM1 (d18:1(4E)/18:0) + CMP-N-acetyl-beta-neuraminate = ganglioside GD1a (18:1(4E)/18:0) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73110"/>
            <dbReference type="ChEBI" id="CHEBI:90153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:48249"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="28">Homodimer; disulfide-linked. Homodimer formation occurs in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.2" evidence="18"/>
    <dbReference type="EC" id="2.4.3.4" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01024310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HG531977">
        <property type="protein sequence ID" value="CDI70965.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001305634.1">
        <property type="nucleotide sequence ID" value="NM_001318705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHF3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007812"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007822.1">
        <property type="protein sequence ID" value="ENSLOCP00000007812.1"/>
        <property type="gene ID" value="ENSLOCG00000006466.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688818"/>
    <dbReference type="KEGG" id="loc:102688818"/>
    <dbReference type="CTD" id="445567"/>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166842"/>
    <dbReference type="HOGENOM" id="CLU_032020_2_1_1"/>
    <dbReference type="OMA" id="HSFIIRM"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006466">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047288">
        <property type="term" value="F:beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003836">
        <property type="term" value="F:beta-galactoside (CMP) alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23966">
        <property type="entry name" value="GT29_ST3GAL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000002">
        <property type="entry name" value="CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051757">
        <property type="entry name" value="Beta-gal_alpha2-3_sialyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032">
        <property type="entry name" value="ALPHA-2,3-SIALYLTRANSFERASE ST3GAL I ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032:SF5">
        <property type="entry name" value="ST3 BETA-GALACTOSIDE ALPHA-2,3-SIALYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 37" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="36" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 37" id="KW-0808">Transferase</keyword>
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</entry>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
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                <person name="Jaffe D."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the SNAPIN family.</text>
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    <sequence length="199" mass="22569" checksum="057BAC5F707BE143" modified="2014-04-16" version="1">MHLVLLRHHDRFVYISCPPLPPPRHSFHRCQKTRRLHFPRVIAAHSSCRAYKHCHSGLHFPVGSVEGIAGMAAVAVIETPSGKDAIAEGLLDLLKPAVQQLDMHVHSVRESQVELREHIDNLATELCRINDHQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRKTMLETTGAFVPRSPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIH2</accession>
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        <submittedName>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000008191.1">
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        <property type="gene ID" value="ENSLOCG00000006767.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3784">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="HOGENOM" id="CLU_028138_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIH2"/>
    <dbReference type="OMA" id="KARFRWH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006767">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13338">
        <property type="entry name" value="FERM-like_C_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23070">
        <property type="entry name" value="PDZ_SNX27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000061">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.1520.10:FF:000003">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037827">
        <property type="entry name" value="SNX27_FERM-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF19">
        <property type="entry name" value="SORTING NEXIN-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="40"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="158"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="52977" checksum="F6DF70C8CD5744D7" modified="2014-04-16" version="1">MADEEGDGTRSGPPSLLHPPPKSGSGPCGTATTVASGPRLVRIVKSDSGYGFNVRGQVSEGGQLRSINGELYAPLQHVSAVLPGGAADRAGISKGDRILEVNGVSVEGATHKQVVDLIRAGERELVLTVLSVPQPEADSLDPGDDSSGQSFYDYTEKQAVPISVPTYKHVEQGSEKFVVYHVYMAGRQLCSKRYREFAILHQNLKREFASFTFPKLPGKWPFSLSEQQLDARRRGLEEYLEKGGSFLRGRGAGQGERWEEGQGRGQXSLPLAPPVCVSPPLPVRSVTQAVPLSLQAVDDVKKGFIKAEEKAYQLQKLAEQRKMTMYLSVLQSCEGYNEIIFPHCACDSRRKGHVITAISIQHFKLHACTEDGTLENQVIAFDWAEMQRWDTDEEGTAFCFEYARAEKKPRWVKIFTPYFNYMHECFERVFCELRWRKEVSPNPTARGLKGLSLSEYLPAVEVTQKGWRHLGGEIVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIP6</accession>
    <name>W5MIP6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein kinase, interferon-inducible double stranded RNA dependent activator</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRKRA family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020106">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214429.1">
        <property type="nucleotide sequence ID" value="XM_015358943.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIP6"/>
    <dbReference type="FunCoup" id="W5MIP6">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008255"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008265.1">
        <property type="protein sequence ID" value="ENSLOCP00000008255.1"/>
        <property type="gene ID" value="ENSLOCG00000006829.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684275"/>
    <dbReference type="KEGG" id="loc:102684275"/>
    <dbReference type="CTD" id="8575"/>
    <dbReference type="eggNOG" id="KOG3732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157618"/>
    <dbReference type="HOGENOM" id="CLU_048292_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIP6"/>
    <dbReference type="OMA" id="PEYEFEK"/>
    <dbReference type="OrthoDB" id="10056847at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006829">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19863">
        <property type="entry name" value="DSRM_PRKRA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19892">
        <property type="entry name" value="DSRM_PRKRA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000007">
        <property type="entry name" value="Double-stranded RNA-binding protein Staufen homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000005">
        <property type="entry name" value="Putative double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044467">
        <property type="entry name" value="PRKRA_DSRM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF2">
        <property type="entry name" value="INTERFERON-INDUCIBLE DOUBLE-STRANDED RNA-DEPENDENT PROTEIN KINASE ACTIVATOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="122"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="306" mass="33166" checksum="61F27D962CCC4A27" modified="2014-04-16" version="1">MSQERFPAASKRSSVDSVKQMVTGCPGKTPIQILHEYGTKTGKMPVYAMEKEEGDAHLPTFIFSVTVGCITCTGQGNSKKAAKHTAAEAALNILKEVPDGDGHSLPRTEDCIIAKHPQNQTNSIGVLQEMSVHKGWRLPEYSVSVEAGPPHKKEFTVTCKVEAFTEMGTGSSKKVAKRIAAEKMLAKLQSLSESCVISMMPKTTGFRLESLRNSSAEKITMLKRNPLSIPNTDYVQMMADLCEEQGFEITYLNIDELTVNGQYQCLAELATSPVIVCHGTGITCGNAHNDAAHSALQYVKIVAGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJF5</accession>
    <name>W5MJF5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RAB22A, member RAS oncogene family</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024237">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639721.1">
        <property type="nucleotide sequence ID" value="XM_006639658.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069035609.1">
        <property type="nucleotide sequence ID" value="XM_069179508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJF5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008514"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008524.1">
        <property type="protein sequence ID" value="ENSLOCP00000008514.1"/>
        <property type="gene ID" value="ENSLOCG00000007029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694573"/>
    <dbReference type="KEGG" id="loc:102694573"/>
    <dbReference type="CTD" id="57403"/>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157009"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="InParanoid" id="W5MJF5"/>
    <dbReference type="OMA" id="SESHRTC"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007029">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
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    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000346">
        <property type="entry name" value="RAB31, member RAS oncogene family"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="Small_GTPase"/>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
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    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="194" mass="21745" checksum="1C02EB971A9B5707" modified="2014-04-16" version="1">MALRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQERFRALAPMYYRGSAAAIIVYDITKEESFQTLKNWVKELRQHGPPNIVVAIAGNKCDLTDAREVPEKEAKDYADSIHAIFVETSAKNAININELFIEISQRIPVTDPSMGAAGKGFKLRRQASESRRSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJM1</accession>
    <name>W5MJM1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">T-lymphocyte activation antigen CD86-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196720.1">
        <property type="nucleotide sequence ID" value="XM_015341234.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJM1"/>
    <dbReference type="SMR" id="W5MJM1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008590.1">
        <property type="protein sequence ID" value="ENSLOCP00000008580.1"/>
        <property type="gene ID" value="ENSLOCG00000007086.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686603"/>
    <dbReference type="eggNOG" id="ENOG502S7QF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067879"/>
    <dbReference type="HOGENOM" id="CLU_939961_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJM1"/>
    <dbReference type="OMA" id="CASHNGY"/>
    <dbReference type="OrthoDB" id="9904387at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007086">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="HERV-H LTR-associating 2b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000910">
        <property type="entry name" value="T-lymphocyte activation antigen CD80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF2">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN CD86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="138"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="296" mass="33098" checksum="36C979BAC8C387A5" modified="2014-04-16" version="1">MNNKKDSTIFYFLCIFIYLIPLLQSISSDKIMAYVGGNIDLPCTSQKNGSEIKFLYMQKYANEDPLFVNGIHKERSTKEQISPVFRNRTSLNEFSKTVNLWDIRITDEGMYDCIIMYYPDVQVSSKFWLTVLVNYSTPVLTVPMNSSGNSTGAGCLIEITCSSSGGYPQKNIKWNLHGNESSLEKVNESIREDPVTRLFNISSTISVNASGPINVSCSVGEETSPEYEACMVKIHPPDPPTDDNSLHIAVALIGLFVIILITVLANKMKNRQVHLQEGDTHRNEHESIVLTGIVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJM7</accession>
    <name>W5MJM7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">G protein-coupled receptor 157</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJM7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008586"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008596.1">
        <property type="protein sequence ID" value="ENSLOCP00000008586.1"/>
        <property type="gene ID" value="ENSLOCG00000007093.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU1X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012992"/>
    <dbReference type="HOGENOM" id="CLU_121723_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJM7"/>
    <dbReference type="OMA" id="WGTVRFV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007093">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF47">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="173" mass="19332" checksum="268E7BE6E9EF4BF4" modified="2014-04-16" version="1">SWGVPLGITVAAVVLQKIGYDASEVSVGWCWVRIQAPDHVLWMLLTGKIWELLAYLALPVLYVLIKKHISKAHAALSEYRPILANSPLSRSRSSLADRKLTLIPIIFIALRVWSTVRFFLLLSGSPAVHNAVLVTLHVIKHIAMPSSCCCFFLLLNESIRSRVLLIGFDRHPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKK8</accession>
    <name>W5MKK8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. ICAM family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKK8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008917"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008928.1">
        <property type="protein sequence ID" value="ENSLOCP00000008917.1"/>
        <property type="gene ID" value="ENSLOCG00000007361.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159005"/>
    <dbReference type="HOGENOM" id="CLU_344730_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKK8"/>
    <dbReference type="OMA" id="ADYELHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007361">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013768">
        <property type="entry name" value="ICAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047012">
        <property type="entry name" value="ICAM_VCAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003987">
        <property type="entry name" value="ICAM_VCAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771:SF9">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03921">
        <property type="entry name" value="ICAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01472">
        <property type="entry name" value="ICAMVCAM1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="88"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="292"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="499"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="607"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="682" mass="74891" checksum="945930D370557F66" modified="2014-04-16" version="1">CPVEISPQRAVVRYGDPVTAKCTTTQTARGIGWEATVGATGMKTDVQSVTWNVSSLTDWTAEPKCYGNFLTEPKQCGKNLIIILYKYPDSVSLSSDSPVDQMVEGIQYQLQCEIKNIAPIQNLTVNWYRGQTLIHNQTFSHNTTRTPGNVSATLLFTPDRADNGQQLTCEAELNLGPEGPQPPPTNQSDPLSITMLLCPGAVPECPLEISPQRAVVRYGDPVTVNCTATQTPRGMGWEAPVGSTGMRKDVQSVIWNVSSLTHWTAEPKCFGNFNTEPRQCRKNLNIRLYKYPDSVSINSGSPVVQMVEGRQYQLQCEVQNIVPVQYLTVNWYRGQTLIHSQTFSHNTTRTPVNVSAALLFTPDRADNGQQLTCEAELNLGPEGPQPPPAKQSDPLLITLKALRSIGLSSEDCPIQIRPSRLVVRYGDPVRAECTATQPLRGMGWEATAGPTGMQIVSQVVTWNVSSLTDWTSEPKCFGNFYQTPKQCEKKLTIVLYKLPDSVSLNQTVQMVEGRQYQLQCEVQNIAPVQYLTVNWYRGKTLIHSRTFSHNTTRTPVNVSATLLFTPDRADNGQQFTCEAELNLGPEGPQPPPAKRGKPVMITVLYKPVFTSTGPTAVQVPPGTRIFLNSSADGNPPPTFEWKYSSASRVQVTTNRDTSVAAVTVNSEADAGAYTCIAANTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKR1</accession>
    <name>W5MKR1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myeloid leukemia factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034783">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKR1"/>
    <dbReference type="FunCoup" id="W5MKR1">
        <property type="interactions" value="854"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008970"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008981.1">
        <property type="protein sequence ID" value="ENSLOCP00000008970.1"/>
        <property type="gene ID" value="ENSLOCG00000007392.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005023"/>
    <dbReference type="HOGENOM" id="CLU_063313_3_0_1"/>
    <dbReference type="InParanoid" id="W5MKR1"/>
    <dbReference type="OMA" id="FDNEWRR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007392">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019376">
        <property type="entry name" value="Myeloid_leukemia_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13105">
        <property type="entry name" value="MYELOID LEUKEMIA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10248">
        <property type="entry name" value="Mlf1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="206" mass="22762" checksum="38979411812DFB31" modified="2014-04-16" version="1">DPFTAHRQHMRSLFGSFGFDPFPLSPQIQPSRTPQIQAAGALSPFGMMGAGGGFMDMFGMMSGMMENMERMSGSQMYSSSTVISYSSSGSGAPKVYQQTSEMRAAPGGIRETLQTIRDSESGLERMSIGHHIRDRGHVMERSRNRKTGDREERQDFINLEESEAAAFDEEWRRETARYPPPSLRGLDYGRERSGGGRGGQLALPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML77</accession>
    <name>W5ML77_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lysosomal amino acid transporter 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PQ-loop repeat-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 66 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Amino acid transporter that specifically mediates the pH-dependent export of the cationic amino acids arginine, histidine and lysine from lysosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192709.1">
        <property type="nucleotide sequence ID" value="XM_015337223.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192710.1">
        <property type="nucleotide sequence ID" value="XM_015337224.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192711.1">
        <property type="nucleotide sequence ID" value="XM_015337225.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192713.1">
        <property type="nucleotide sequence ID" value="XM_015337227.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML77"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009136"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009147.1">
        <property type="protein sequence ID" value="ENSLOCP00000009136.1"/>
        <property type="gene ID" value="ENSLOCG00000007530.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682271"/>
    <dbReference type="KEGG" id="loc:102682271"/>
    <dbReference type="CTD" id="54896"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003344"/>
    <dbReference type="HOGENOM" id="CLU_019699_3_0_1"/>
    <dbReference type="InParanoid" id="W5ML77"/>
    <dbReference type="OMA" id="DMCIFIQ"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007530">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080144">
        <property type="term" value="P:intracellular amino acid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000013">
        <property type="entry name" value="lysosomal amino acid transporter 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000017">
        <property type="entry name" value="lysosomal amino acid transporter 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF36">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="32472" checksum="5E43987F293EA81B" modified="2014-04-16" version="1">MPSGREQDGNFSVWCPNGSQWVWEGLQECAQDARDMASVALGLLSILCFMTSSIPQYVSSCRRGNMDQALSVWFLVFWLAGDTCNLTGSFLADQLPLQTYTAVYYVLADLLMIGMFNYYKFKNRRHSNGVVLNAVGVASLAGVSASLTLFLGAGPSVDAPLGVGGRVLLSVPSGDSGIEPFQTKEIIGFVIGSMSSVLYLCSRLPQIYTNFKRKSTEGVSYFLFALVILGNTTYGLSVLLKNPDHGHRELSYIVHHLPWLIGSLGTLSLDIVITVQFLMYRGPRSRKGEESEALISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLV7</accession>
    <name>W5MLV7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zic family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015276">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637873.1">
        <property type="nucleotide sequence ID" value="XM_006637810.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLV7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009366"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009377.1">
        <property type="protein sequence ID" value="ENSLOCP00000009366.1"/>
        <property type="gene ID" value="ENSLOCG00000007717.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696592"/>
    <dbReference type="KEGG" id="loc:102696592"/>
    <dbReference type="CTD" id="84107"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154200"/>
    <dbReference type="HOGENOM" id="CLU_002678_37_3_1"/>
    <dbReference type="InParanoid" id="W5MLV7"/>
    <dbReference type="OMA" id="PASHHGM"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007717">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000039">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="394"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="459"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="417"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="491" mass="53645" checksum="B42A0A7592E70EB1" modified="2014-04-16" version="1">MSVDALGNPMMDPAFSKRNTALRLVDLAGAHHHHHHHHTPQSMTGFPGFSSHPHSMAHTHPGEITSEPRLGPSPFGPEHMGHSAALKLSPAHHYSHHHHHHNHHMAGHSEVVSSQTGAFGPVQAAAVPYSMSHTAQALTAGSYPGHYGHHPEAGNHNLFPGLHHEQPPNGAPGGQALNGQIRLGIPGEMYVRSDHLSQVAGSRADRFTASPLHGYSGMNLNMNLGAHHHGPGAFFRYMRQPIKQELICKWIEPEHAAKKLCSKTYSTMHELVTHVTVEHVGGPEQANHICFWDDCPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFDGCDRRFANSSDRKKHSHVHTSDKPYNCKVRGCDKSYTHPSSLRKHMKVHCKSPPPSSGYESSTPSLVSPSSDSGRDPASSSHSEPLSSSQAANLSEWYVCHSSGASGIPTPPSKPASPDSADVPPYRNPEPGDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM56</accession>
    <name>W5MM56_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin accessibility complex protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA polymerase epsilon subunit p15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1. Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer with POLE3; binds to DNA. Component of the CHRAC ISWI chromatin remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity. Within the complex, the heterodimer with POLE3 interacts with SMARCA5/SNF2H; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction. Within the complex, the heterodimer with POLE3 interacts with BAZ1A/ACF1; the interactions are direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635857.1">
        <property type="nucleotide sequence ID" value="XM_006635794.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM56"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009465"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009476.1">
        <property type="protein sequence ID" value="ENSLOCP00000009465.1"/>
        <property type="gene ID" value="ENSLOCG00000007800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685204"/>
    <dbReference type="KEGG" id="loc:102685204"/>
    <dbReference type="CTD" id="54108"/>
    <dbReference type="eggNOG" id="KOG1657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166127"/>
    <dbReference type="HOGENOM" id="CLU_045277_11_0_1"/>
    <dbReference type="InParanoid" id="W5MM56"/>
    <dbReference type="OMA" id="EDIHSCS"/>
    <dbReference type="OrthoDB" id="1291358at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007800">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22924">
        <property type="entry name" value="HFD_CHRAC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000048">
        <property type="entry name" value="Chromatin accessibility complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="13">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="116" mass="12960" checksum="9BC0B7906BAEB0BD" modified="2014-04-16" version="1">MAGNNVDEKQNHTSNKHISLPISRVKLIMKSSPDVSSINQDALFLTAKATELFVQHLATYAFKKGKGKEKNSLTYSDLANAAEESETFQFLADILPRKILARDYLKSLEEKGEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPT1</accession>
    <name>W5MPT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Periostin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Osteoblast-specific factor 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with BMP1 and fibronectin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPT1"/>
    <dbReference type="FunCoup" id="W5MPT1">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010390"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010404.1">
        <property type="protein sequence ID" value="ENSLOCP00000010390.1"/>
        <property type="gene ID" value="ENSLOCG00000008539.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063860"/>
    <dbReference type="HOGENOM" id="CLU_017611_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPT1"/>
    <dbReference type="OMA" id="QWLSYHI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008539">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071307">
        <property type="term" value="P:cellular response to vitamin K"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000001">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000002">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000003">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000004">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016666">
        <property type="entry name" value="TGFBI/POSTN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF12">
        <property type="entry name" value="PERIOSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016553">
        <property type="entry name" value="BIGH3_OSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="3" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868539" description="Periostin" evidence="17">
        <location>
            <begin position="23"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="19">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="18">
        <location>
            <begin position="91"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="18">
        <location>
            <begin position="234"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="18">
        <location>
            <begin position="368"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="18">
        <location>
            <begin position="496"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="821" mass="90198" checksum="EA9D90C673D57EC6" modified="2014-04-16" version="1">MKLLTVAAVTIFVLSAFDQADSSAYDKIVTHSRIRAKNEGPNVCALQQVMGTKKKYFSTCRNWYQGAICGKKATVLYECCPGYMKLDGMRGCPAVAPIDNVYGTLGLVNAITTQDYSDRSKLREEIEGHGSYTFFAPSNEAWDLLDPEVRSGLVSNVNIELYNALHYHMVNKRLLTKDLKNGMTVESMYDKQGLLINHYSNGVVTVNCARVIHGNQIATNGVVHVIDRVITGVGNTIQDVIEVDDDLTSLSAATVASGLLDKLGEPGHYTVFAPTNEAFEKLPRAVLERIMSDKVALQALLKFHILDSVQCSEAIMAGSSYETLEGNNIEIGCDGESLTVNGIKMVKRKDVVTSNGVIHFIDQVLIPNSAKQVLELTGPSQSTFTDMIAQLGLAGSFRPEAEYTLLAPLNGAFTDEVLAMDQRRLKIILQNHILKLKVGLSQLYNGQLLETLGGKILRVFIYRTAVCIENSCMVRGSKEGRNGAIHLFREIITPAEKSMFEILKSDARFSMFLQLMDVAGLTEVLKQKGDWTLFAPTNDAFEGLTQKEIEILTSDPNALKTILLYHLSNGVFIGGGMESGVTNLLKSMAGSNLMVKAINNTILVNEVKADKSDVMATNGVVHIVKSLLYPRDIPVGNQQLLTILKRLIKHIQLKFIQGYRYQEIPLTFLTKIIKTEVREPEIKIIGGGTQTVTRVIQGGPQITKITRVVDGQPSITKVTRVIEGEPAITKVTRVIEGDPALTKVTRVIEGDPTLTKVTRVIEGTVSPGPQYAVTHVSTDGQHEIDGEIKKILSEEATKVTKLLESEAHLLEEEEIKRLLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ03</accession>
    <name>W5MQ03_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ddb1 and cul4 associated factor 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010462"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010477.1">
        <property type="protein sequence ID" value="ENSLOCP00000010462.1"/>
        <property type="gene ID" value="ENSLOCG00000008600.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182900"/>
    <dbReference type="HOGENOM" id="CLU_012381_0_1_1"/>
    <dbReference type="InParanoid" id="W5MQ03"/>
    <dbReference type="OMA" id="TICWNDR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008600">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000045">
        <property type="entry name" value="DDB1- and CUL4-associated factor 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF39">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="663"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="739"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="980" mass="109583" checksum="741961EA4E62EBDF" modified="2014-04-16" version="1">MSCSDNLLWDVRKRSIGFDHPNAVRINYLGRREFVQRLKLEATLNVHDGCVNTICWNDTGEYILSGSDDTNLVITNPYNRKVKATIRSGHRANIFSAKFMPQTNDQQIVSCSGDGIIFYTNTEKSPEINRQCQFTCHYGTAYEIITVLNDPYTFLSCGEDGTVRWFDVRMKTSCTKEECKDDILINCRRAATSISICPLVPYYLAVGCSDSSVRIYDRRMLGTRATGNYMGRGTTGMCVRFVPAHLSNKSCRVTSLCYSGDGQEILVSYSSDYIYLFDPKDDQARELKGPSEERREELRQPPVKRLRLRGDWSDTGPRARPESERERDGEQSPNVSLMQRMSDMLSRWFEEASEVAQSSRGRPQPRPRELIIRIFFPSNPLTLIPFLSLRALLHVRLLLVYAFLYHHCSPHAPFPPQTXLMSSTASSSRGSGGHSKLDKPEEPQKTQSKEESSLESMEAVQISLSSAYSSINITLSPAISSIKINSNSISHDNPNQPTTCLAVQLGKVIIVSYTPLAIVQSAIITPLTKFNSDNCLQKNALSSSENYHKKPQLLLLMLILELPLRNQGSNNLMFLFTKSSSLENSSMEQQSIVHGNAHFPYRCILDQIIFNTVSKKKKDTTIRFLGDFHMSGPAECITEMFLDDLKCICLCVAAPSALAEKPMQSKALEPPEQTGAGPAGAGAGGEESTDSSTEKASPSSPESPAAGPAEELAQGADSASSQPPEEQQAGAGLENPSESCRRTEPAGESSREQATRSYQDSDDSDDDPILIPSARYRAGQGQRFNFRGSAVGDRMIRRSAAARIQELFRRRKERREMEENETLNIRRPAVKMVYKGHRNSRTMIKESSFWGSNFVMSGSDCGHIFIWDRHTAEHLMLLEADNHVVNCLQPHPFDPILASSGIDYDIKIWSPLEESPSFNRKLAEEVITRNELMLEETRNTITVPASFMLRMLASLNHIRADRLEGDRSEGSGQENENEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQC1</accession>
    <name>W5MQC1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CREB regulated transcription coactivator 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TORC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010580"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010595.1">
        <property type="protein sequence ID" value="ENSLOCP00000010580.1"/>
        <property type="gene ID" value="ENSLOCG00000008695.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVWA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010652"/>
    <dbReference type="HOGENOM" id="CLU_019357_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQC1"/>
    <dbReference type="OMA" id="WARHALP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008695">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008140">
        <property type="term" value="F:cAMP response element binding protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071320">
        <property type="term" value="P:cellular response to cAMP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024786">
        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024785">
        <property type="entry name" value="TORC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024783">
        <property type="entry name" value="TORC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF6">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12886">
        <property type="entry name" value="TORC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12885">
        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12884">
        <property type="entry name" value="TORC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity N-terminal" evidence="11">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="12">
        <location>
            <begin position="169"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity C-terminal" evidence="13">
        <location>
            <begin position="719"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="378"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="487"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="378"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="527"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="786" mass="83337" checksum="3A42663DF3E04557" modified="2014-04-16" version="1">MSAAGAGGCGPGPGSGAGSGASNPRKFSEKIALHTQRQAEETAAFQEVMMDISSTRVSLESGRQQRERETHPGGGSNFLLLTDNTPFHFEGPFLSSLDSSRGTRHHGLVERVQRDRRFMSPLRHYRRQIDSSPYSAAYLSPPPDASWRRFWEAGYCADLLPNESRNRLRTNSDSALHTSVMNPNAQDAFGSGPGLAPQNRHSVGLIEGDGRKSKPSVICFLLPRFLNRGVALQWMYTSRVPMLSSRPKSCEVPGINPFSPSGCPLPPSSAVLSVGDRCGLRVSTGGKHFQPPLPTPLDPEEPPFPSLSGGSSTGNLASTLTQLGINPGGAYHSPVLSSSLQGNLSNPTLQSSLSNPNIQSSLSSHSFQSSLSSASLHSSLSNPSLQSSLSSSPSLQSSFSNQSLQSSLSNSSLSGQSIQSSASNPSYSSGVGSSASSSSSSSSSYTPPMSGQQQLSLSTSPRRRAQLSPLILPMGGDSRRHHVKQFSPTISPTLSSITQGVPLDTSKLPPYPYSQGGPQPPGPPTSHQPLQKGGASSPAGQLQQQQQQPPSSQHPLHPQQPSGAHPAQSQSQPPLPPPGQQPQQQHAHLQAQSAQGMRQPQDWLPSASERHAARGYLLHVTHMRERARKMNDDSSLLNSLLDDPYLDLQLTAQQTQVLSQQARAIAGRRVGLGDGSCEPIQLVKDSMASLAFSGPSDPHVLNKHNQSYGCGVGRHGNVPNIILTGDSPPGLSKEITSALAGVPGFEVDPFSLDDSLALEGLGILADGDLMLPDPSVEDSFRSDRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRH2</accession>
    <name>W5MRH2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Villin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000010997.1">
        <property type="protein sequence ID" value="ENSLOCP00000010981.1"/>
        <property type="gene ID" value="ENSLOCG00000008995.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102696587"/>
    <dbReference type="GeneTree" id="ENSGT00940000160544"/>
    <dbReference type="HOGENOM" id="CLU_002568_3_1_1"/>
    <dbReference type="OrthoDB" id="6375767at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008995">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120035">
        <property type="term" value="P:regulation of plasma membrane bounded cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000004">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000005">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000027">
        <property type="entry name" value="Villin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000005">
        <property type="entry name" value="Villin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF35">
        <property type="entry name" value="VILLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0117">Actin capping</keyword>
    <keyword evidence="12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="15">
        <location>
            <begin position="759"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="825" mass="93195" checksum="AD974871700F704B" modified="2014-04-16" version="1">MPQVKSQVSKVLNKTTPGLQIWRIENMEMVPVPPKSYGNFFEGDSYIILYTHKTSNNYTYDIHYWLGKASSQDEQGAAAIYTTQMDEHLGGTAVQHREAQGYESETFKGYFKQGLXYKKGGVASGMKHVETNTYNIKRLLHVKGKKNVVAGEVEMSWKSFNKGDVFLLDLGSFIIQWNGPESNRMERLKGMNLAKDVRDRERGGRAQVGVVEGDNEGASAELMKVMNHVLGERKELKPAIPDEVVDQTMKSSIKLFHISEAEGSLVVQEVAVKPLTQDLLKHEDCYLLDQGGIKIYIWKGKKASKKERTESLEKAEAYKKAKGYPPSTYVETVNDGAESAVFKQLFQKWTVKGQTSGLGKTHSVGKVAKVEQIKFDATSMHARPDLAAQQKMVDDGTGEAEVWRIEDLEPVPVEKKWLGHFYGGDCYLILYKYEVNNKLNYILYIWQGRHASKDEITASAYQAVILDQKYDGKPVQVRVPMGKEPPHLMAIFKGKMVVYEGGTSRAVSKEPERPVRLFHVHGTNEFNTRAIEVPARSSSLNSNDVFVLKTETCCYLWYGKGCSGDEREMAKTVTDIISKREKHVIAEGQEPADFWVTLGGKSQYSNSKRLQEKSSVITPRLYECSNQTGRFIATEITNFNQDDLDEDDIMLLDIWDEIFLWVGKGANETEKKEVVVTAQEYLQTHPGGRDPDTPIILVKQGFEPPTFTGWFLAWDPHKWSDGKSYEELKAELGNSSAIIEITVDMMKPNHKADNINMSSAVLPTFPAEKLVNCQVEDLPEGVDPSRREEYLSNNDFTDVLGMTRPEFYAMPLWKQKNLKKAKGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRW3</accession>
    <name>W5MRW3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP synthase mitochondrial F1 complex assembly factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRW3"/>
    <dbReference type="FunCoup" id="W5MRW3">
        <property type="interactions" value="840"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011138.1">
        <property type="protein sequence ID" value="ENSLOCP00000011122.1"/>
        <property type="gene ID" value="ENSLOCG00000009121.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012765"/>
    <dbReference type="HOGENOM" id="CLU_067356_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRW3"/>
    <dbReference type="OMA" id="MFYYKTD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009121">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="308" mass="34862" checksum="BC7FC50A9AE68364" modified="2014-04-16" version="1">LLMYDGGEGKMAVAVVQMACLYRGMLAVRNTGIRPLIPGFSPLQLRAFSVRKEPELEENPFYSKYQDKIQQLRKAKPEEYKSRLEKRAEGEKLPVGHCKQAEFIKLIEAELEKRSKGAPGSDGFTKDKTLDSILNIDMVKEKTGEEIAQIWKEYFATKKTICAVIPGGIFETIFRRAKKCPSFLYALPQKEGYEFFLGQWSGLELHFTSLINVQTLGENAPSQLILYHYPEFEKEKGIVLMTAEMDPKCMGVHQAQCLANQVQLFYGAKRKETFQLVDTFNHRPAEFKHMSVIAELEQSGIGPGAVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSR4</accession>
    <name>W5MSR4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">COBW domain containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSR4"/>
    <dbReference type="FunCoup" id="W5MSR4">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011423"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011440.1">
        <property type="protein sequence ID" value="ENSLOCP00000011423.1"/>
        <property type="gene ID" value="ENSLOCG00000009357.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00640000091523"/>
    <dbReference type="HOGENOM" id="CLU_017452_0_1_1"/>
    <dbReference type="InParanoid" id="W5MSR4"/>
    <dbReference type="OMA" id="HSQGFET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009357">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140827">
        <property type="term" value="F:zinc chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="8">
        <location>
            <begin position="270"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="43520" checksum="D550E7BBE038A3BE" modified="2014-04-16" version="1">VLCPVVCVDEFADKAAEGDDCPVLVPIENELNTQAAQIPVTIITGYLGAGKTTLLNYILTEQHNKRIAVILNEFGEGSALEKSLAVSQAGELYEEWLELRNGCLCCSVKDNGLKAIENLMEKKGKFDYILLETTGLADPGAVASMFWVDAELGSDIYLDGIVTVIDAKYAMQHLTEEKPEGQINEAARQIALADLAIINKTDLVGEEELCRLREVVRSTNGLVRIIETQKSRVDLSSVLDLHSFDSKAGASLQEKLLHIQTTQPHLDKSIVTVTFEVPGCISEERLNVFIQNLLWEKTIKNKTGLLMNVIRLKGILSIQNKPQKVILQSVHELYDLEETPQIWDSEEPRISRLVFIGKNLDKGILEELFITTVLNQTSNTFTFSNKDENCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT13</accession>
    <name>W5MT13_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Low density lipoprotein receptor-related protein 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <location evidence="12">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MT13"/>
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    <sequence length="853" mass="93924" checksum="89DA45229B256DD6" modified="2014-04-16" version="1">LAFTFFQQQSVPYERNEALKFFFLSYLGNAVHADHNENVYVPGISNVCGDVAEQIRASSGVITSPGWPFEYPSRINCSWNIRANPGEIITISFQDFEIQSSRRCGSDWISIGTSKNLDGYRACGSSIPAPYISSQDHVWIKFHSDDSQTGKGFRLSYITGKSEEISCDSDQFHCANGKCIPESWKCNTMDECGDNSDEELCLQPNPSAAFSFQPCAFNQFPCLSRYTRVYTCLPESLKCDGSIDCQDLGDEIDCDVPTCGELLRNFYGTFSSPNYPDFYPPGSNCTWLIDTGDHRKVILRFMDFKLDGTGYGDYVKVYDGLEENPRRLLRVLTAFDSRAPVAVVSSSGQIRVHFYADKINAARGFNVTYQVDGFCLPWEIPCGGNWGCYTEQQRCDGYWHCPNGRDEVNCSTCQEDEFPCSRNGACYPHSDRCNYQNRCPNGSDEKNCFFCQPGNFHCKNNRCVFESWVCDAQDDCGDSSDEENCPVIVPTRVITAAVIGSLICGLLLVIALGCTCKLYSLRMFERRSFETQLSRVEAELLRREAPPSYGQLIAQGLIPPVEDFPVCSANQASVLENLRLAVRSQLGFTSIRLPTSGRNSNIWRRLFNFTRSRRSGSLALVSADEGAGNSSTAHEAERIGPQRGLLPLDSDDTDTEGDRRDMPGAVGGLVAPLPQKVAPVSAVEAIVSVSVSSSRQASRDPAESAREAGTAEPRCAAPGRNQLSSALSRVTRSLRWVRFSLGRSSSASQSQSPLRHLDHASGGREDDDDVELLIPASDAASDSDLSECSRPLLEPTPEHGRAPVQPTAPRARPPSRDGPCEHCGMVHTAQIPDACLEATVKTETSDDEALLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV35</accession>
    <name>W5MV35_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Zinc finger Ran-binding domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May interfere with constitutive 5'-splice site selection.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the ZRANB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000012244"/>
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        <property type="protein sequence ID" value="ENSLOCP00000012244.1"/>
        <property type="gene ID" value="ENSLOCG00000010010.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Linkage group LG10"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:lipid antigen binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000007">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Znf_RanBP2"/>
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    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017337">
        <property type="entry name" value="ZRANB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999:SF17">
        <property type="entry name" value="ZINC FINGER RAN-BINDING DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999">
        <property type="entry name" value="ZINC FINGER RAN-BINDING DOMAIN-CONTAINING PROTEIN 2 ZRANB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn_ribbon_RanBP"/>
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    </dbReference>
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        <property type="entry name" value="UCP037956_ZnF_Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="113"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="38450" checksum="2ABD95D9A958F299" modified="2014-04-16" version="1">MSGKSFRVSDGDWICPDKKCGNVNFARRTNCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCGNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKTVGATSILKDKEKEQAGEENDEEDEDDEDDEEGDLSKYKLDDDEDDEEDDDEDADLSKYDLDASDDDAKQEAKKNSRSDSSRSSSRSSRSSSRSRSRSRSRSSSSSRSRTRSSSRDRARSRRSKSSLPRSSSRSRKGSSSPRKRSRSSSPSSSSPERGKKRSRSRSSSSGERKKRRSRSRSSERRRGSSSGSSHSGSQSSSSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWK5</accession>
    <name>W5MWK5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box protein 47</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWK5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012788.1">
        <property type="protein sequence ID" value="ENSLOCP00000012764.1"/>
        <property type="gene ID" value="ENSLOCG00000010408.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV8T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167237"/>
    <dbReference type="HOGENOM" id="CLU_048746_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWK5"/>
    <dbReference type="OMA" id="AFACVTM"/>
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        <property type="component" value="Linkage group LG14"/>
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    <dbReference type="Bgee" id="ENSLOCG00000010408">
        <property type="expression patterns" value="Expressed in testis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056622">
        <property type="entry name" value="ARM_FBXO47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038946">
        <property type="entry name" value="FBXO47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24467">
        <property type="entry name" value="ARM_FBXO47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBXO47 ARM repeats region" evidence="2">
        <location>
            <begin position="180"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24467"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="437" mass="50482" checksum="A205E79A058152F5" modified="2014-04-16" version="1">MEERARCPSGKPAQKSRHQMSRCSVSTRSQSRSTWGLFKGMPAEVFDMVLEHLSVKEMSVFSMVSKAISSHIAGYVSTKAWRRRMILQKFHHPVHKECVLEHYRSLGLLFKRCTLLLPTKERLKFIYSKLSEVPCFEINKCTVTPGCLGFASYGVFLQTLIAGWDELECHRVFSFLCNYTSLPRKLRAAVMGLPGAAPDLEVQIRLFCRSVLLDWWRDQADRLFWLTRLLQPWPFVSQARLLFIFYGPLSQDGKIEWEKMTENVVPECDLWDLAKIIRMLFSESEAKEWTADSVISILDEISASPQFWLRENLARLLILCGNRVCFNFMASKALNGRVMEISRLLVFLILVCEKDNYYMNWSVKMALQIGRLFPERRSFIQNIETAFSQVAMEMCETLMAGGQNADPNLIHNLCSILNANAHFHTEMLCMFIKEYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXV0</accession>
    <name>W5MXV0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal P4.1-associated protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centromere protein J</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Centrosome assembly and centriole elongation protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms homodimers. Associates with microtubules plus ends; binds to beta-tubulin subunits exposed on microtubule outer surface at its distal tip; also associates with microtubule lattice. Associated with the gamma-tubulin complex. Interacts with the head domain of EPB41. Interacts with LYST. Interacts with CEP152 (via C-terminus). Interacts with STIL. Forms a complex with STIL and SASS6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MXV0">
        <property type="interactions" value="1357"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013209"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013237.1">
        <property type="protein sequence ID" value="ENSLOCP00000013209.1"/>
        <property type="gene ID" value="ENSLOCG00000010780.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQR0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063927"/>
    <dbReference type="HOGENOM" id="CLU_008072_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXV0"/>
    <dbReference type="OMA" id="WNEPEDL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010780">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061511">
        <property type="term" value="P:centriole elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902117">
        <property type="term" value="P:positive regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.450.20:FF:000001">
        <property type="entry name" value="Centromere protein J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009852">
        <property type="entry name" value="CENPJ_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047002">
        <property type="entry name" value="Tcp10_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026581">
        <property type="entry name" value="TCP10L/CENPJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058029">
        <property type="entry name" value="Tubulin-bd_CENPJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331:SF27">
        <property type="entry name" value="CENTROMERE PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10331">
        <property type="entry name" value="T COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07202">
        <property type="entry name" value="Tcp10_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25779">
        <property type="entry name" value="Tubulin-bind_CPAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENPJ tubulin-binding region" evidence="14">
        <location>
            <begin position="266"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="977"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1048"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1084"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein J C-terminal" evidence="13">
        <location>
            <begin position="1122"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="28"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="296"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="540"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="707"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="661"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1182" mass="127809" checksum="4DCDB7FC580A1972" modified="2014-04-16" version="1">MSASAGLQPEPSFLARWMSCSPRAGVILDPPPALLSRAGRDGSPAAPGGDDSFSAQFLPLPVSRSSSCLSVDSLGPAGPESAAPGPAARLGSPEELDTAGQRHSAPLMRQLEQVQSSRIHASAQQALAGTASRPPSQAEGQAALAGTASGPGPRALAGTVELASAHHRGAEGRSLWSDDPSGEELCRSSGYEDEPQCSHPAARDEFAPGPAGAASPREGRPLPIREMGHVSSVKTLHISRNTVLMLVCSAIGETGNRSVTEPPGFKDLPIKPGIGGRKQTFEELLEEQMRLEEQRLGENREQGRAAAEVKAQPKRAFLKRGEGLSRFTKGKAAPVKETSKTDAKPQPKTSCQRTVAPRSSQQPVQRKTAILSKESSSERLTGPPNRSQVVSLAKGSKAQNVLGSAAGHSAAVESKQLSVSGQAQEALAVKVQGKKAGGSLPACAGPGKTAPAKQGCSEETKQCVPPKTSMHIPPPEQEYSFELSFQRKLESWEKEKQKENLELDEFELLEQAADEISFSSNSSFVQRVLHLDRLDCRRRRLSSTPIKPPKAVKTGPERSAHDRAARSPPETGDTRTEQARSESVLKDLDKGPGDGNGECDEDSDSSWQSGSASEDMDDTLTGGRAPRVGRARAGGHGCDARVRYDKTSYEDGAVRRGDXGGSPADSGPPLASDLMTRLFPALKPKPKPAPSREDDPVSTAPGHNPQSALLRERLVELETQIERFRAENSALAILRQEREAALEKLRKEVAEFEQKKAEELARLEELKKEEMKKLQKERKVFEKYAAAARAIPDKKEREEMQXLGHRLCSVGSSLTVAPPWVSAFRVSRDKLAGVFSETKGEGSPVESLKYAAESNATCISSVKSLQVTATNSPPPTKTARENSTVAEGTVGGKQNIKQSLLSKMNARLLNNYCNVLVNSIRSVPVPTAVSSIEEQVMSELRKTELHPESPVVLPASTVPTDEPQKDLCGVEENGEILEETIHPDGKIEKVLKSGIRVIVFPNGTQKEVMADGKSVKVTFFNGDVKQILADQRVVYYYADAQTTHTTYPDGLEVLQFPNNQIEKHFPDGRKEITFPDQTIKNLYPDGQEESVFPDGTIIQVKLDGSKVIEFNNGQREVHTAEYKRREYPDGTVKTVYANGQQETKYPTGRVRIKDKDGNIVMDTKVDSEQALLSPHSQSILYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY24</accession>
    <name>W5MY24_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D(1A) dopamine receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Dopamine D1 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY24"/>
    <dbReference type="FunCoup" id="W5MY24">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013283"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013311.1">
        <property type="protein sequence ID" value="ENSLOCP00000013283.1"/>
        <property type="gene ID" value="ENSLOCG00000010837.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155857"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_0_1"/>
    <dbReference type="InParanoid" id="W5MY24"/>
    <dbReference type="OMA" id="THNGQHP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010837">
        <property type="expression patterns" value="Expressed in zone of skin and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001588">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gs"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007212">
        <property type="term" value="P:G protein-coupled dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000045">
        <property type="entry name" value="D(1A) dopamine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001413">
        <property type="entry name" value="Dopamine_D1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF139">
        <property type="entry name" value="D(1A) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00565">
        <property type="entry name" value="DOPAMINED1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="38"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="448" mass="50095" checksum="F711CC5E91ACE40B" modified="2014-04-16" version="1">MDFNITTVIDSDISERDSSARVLTGCFLSLLILSTLLGNTLVCAAVTKFRHLRSKVTNFFVISLAVSDLLVAILVMPWKAITEIAGFWPFGSFCNIWVAFDIMCSTASILNLCIISVDRYWAISSPFRYERKMTPKVAFIMISVAWTLSVLISFIPVQLNWHKAQTTSFSEPNVSYRGLPLDNCDSSLNRTYAISSSLISFYIPVAIMIVTYTRIYRIAQKQIRRISALERAAESAKNRHSSMGNSSSMETESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCMVPFCEHTLPSGNDFPCISSTTFDVFVWFGWANSSLNPIIYAFNADFRRAFSILLGCNRLCPTSNAIETVSINNNGAPPPSFQYQPKGYIPKEGNVTYLIPHSILCQEEESPKKDEEAIGIKTLEKLSPAVSGNLDSEGDVCLEKINPTTQNGQHKTVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYB8</accession>
    <name>W5MYB8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epsilon-sarcoglycan</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <location evidence="4">Sarcolemma</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sarcoglycan alpha/epsilon family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYB8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013377"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013405.1">
        <property type="protein sequence ID" value="ENSLOCP00000013377.1"/>
        <property type="gene ID" value="ENSLOCG00000010901.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4482">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005672"/>
    <dbReference type="HOGENOM" id="CLU_053258_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYB8"/>
    <dbReference type="OMA" id="EPRMFNW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010901">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008908">
        <property type="entry name" value="Sarcoglycan_alpha/epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048347">
        <property type="entry name" value="Sarcoglycan_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048346">
        <property type="entry name" value="Sarcoglycan_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132">
        <property type="entry name" value="ALPHA-/EPSILON-SARCOGLYCAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132:SF17">
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    </dbReference>
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        <property type="entry name" value="Sarcoglycan_2"/>
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    </dbReference>
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        <property type="entry name" value="Sarcoglycan_2_C"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866991" description="Epsilon-sarcoglycan" evidence="19">
        <location>
            <begin position="19"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="20">
        <location>
            <begin position="61"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="449" mass="50479" checksum="FA585DEFF8B22894" modified="2014-04-16" version="1">MRSVGCFICLSVAYLVSSADVKSEAVAWRNSQIHSYFINRQRAAVRTNVFTILSRAHADRNVYPSAGVLFVHVLEREYFKGEFPPYPKPGDASNDPITFNTNLMGYPDRPGWLRYIQRTPFSDGVLYGSPTADHVGKATVIEITAYNRRTFETARHNLVINIISADDFPLPYQAEFFIKNMNVEEMLASEILGDFLGAVKNVWQPERLNAINITSALDRGGRVPLPINDLKEGVYVMVGADVPFSSCLKEVETPQNQLRCSQEMEPLISCDKKFRAQFHIDWCKISLVDITKVVSVSKEKPDPGSGVLPDMGEYNPPSDSLKSRDYFSDFLVTLAVPSAVALVLFIILGYIMCCRREGVEKRNMQTPDIQLVHHSSIQKSSKELRNMSKNREISWPLSTLPVFHPVSGEIVPPLHPDNYETTSMPLMQTQTNLQNQTQIPQQQPAGGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ59</accession>
    <name>W5MZ59_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Solute carrier family 35 member F4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ59"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013668"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013697.1">
        <property type="protein sequence ID" value="ENSLOCP00000013668.1"/>
        <property type="gene ID" value="ENSLOCG00000011125.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008727"/>
    <dbReference type="InParanoid" id="W5MZ59"/>
    <dbReference type="OMA" id="TNCPSSH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011125">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
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        <location>
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            <end position="266"/>
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    </feature>
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            <begin position="272"/>
            <end position="291"/>
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            <end position="319"/>
        </location>
    </feature>
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            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
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            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
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        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="136"/>
            <end position="289"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="490" mass="53493" checksum="1752A44A04704B9F" modified="2014-04-16" version="1">MLIAQSPRPALCPAGPRMSRTAEAGEDGGGDGQPSFALEPLKTTTLPVKASPTLPGRDSDRSPCYTDTIKPTSNGVHTIEDRILRITGYYGYYPGYSSHRREEGSESHADTPGSGDSETPSQCQSCTSTALRVLGGLLLVLSVSSSWVGTTQVVKLTFQSFSCPFFMTWFSTNWNILFFPIYYSGHLVTARQKQTPIQKFRECSRIFGEDGLTLKLFLKRTAPFSILWTLTNYLYLLALRKLTATDVSALYCCHKAFVFLLSWIVLKDRFMGVRIVAAIMAITGIVMMAYADGFHGDSIIGVAFAVGSASTSALYKVLFKMFVGSANLGEAAHFFSTLGFFNLIFISCTPVILFFTKVEHWGSLSALPWGYLCGVAGLWLAFNILVNVGLVLTYPILISIGTLLSVPGNAAVDVLKHEVIFSVVRLAATSIICLGFLLLLLPEEWDSVTLRFLATFAEKKGEEHGEELTESSVHTRSRSRANGAVSIPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZR9</accession>
    <name>W5MZR9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase</fullName>
            <ecNumber evidence="14">2.4.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-[N-acetyl-alpha-neuraminosyl-(2-&gt;6)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81643"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="Rhea" id="RHEA-COMP:19720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="ChEBI" id="CHEBI:231970"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:81644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-[N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81659"/>
            <dbReference type="Rhea" id="RHEA-COMP:14417"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:139598"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:81660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = a beta-D-galactosyl-(1-&gt;3)-[N-acetyl-alpha-neuraminyl-(2-&gt;6)]-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11136"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:140764"/>
            <dbReference type="EC" id="2.4.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11137"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.3" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01000272">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZR9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013878"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013907.1">
        <property type="protein sequence ID" value="ENSLOCP00000013878.1"/>
        <property type="gene ID" value="ENSLOCG00000011290.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159930"/>
    <dbReference type="HOGENOM" id="CLU_032020_4_1_1"/>
    <dbReference type="InParanoid" id="W5MZR9"/>
    <dbReference type="OMA" id="WDFEEQY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011290">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000015">
        <property type="entry name" value="Lactosylceramide alpha-2,3-sialyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941:SF1">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="34"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="495" mass="56758" checksum="1F60225D4C82D041" modified="2014-04-16" version="1">MRRSFVHTRGSEFSKETASVLFLDENQGRSQHALETLGSNGTLKASLDKKAETTNKRIEKDKVESDDNLKPKALSSKTFPGSKTTKDTPKPSKVGTTTKRPQEKSTTAKSKKVTLTRGTKANRMSPAEDKLEGRTKSMTPTPLVPPMNKLDFKKIPKWDFEDIYRRDSHPRQTTCPESVMLSKDPDFQKALIPDIKIFMHKEDFNTSEWNRLYHFNNPFGFMGGNYTEVKEAVQLIPKLKDYQLLPVPKNEAHGCIRCAVVGTGGILNGSRMGKEIDSHEYVFRMNGAVTLGHEDDVGNKTSVYVHTAHSLSSSISLFKRHGMSGIPHNEGTKYVMIPEGMRDFQWLIGLLKNTTVATGPYRGRKLWTYYPSQFDPNKFYVLHPDFLRYIRNRFLNSTQLQSPHWHIFRPTNGAFTLFLALHVCDVVDAYGFITADHAKYPNYYCDKQKSRVVFYINHDYNLEIRTWKKLHDTKILKLYQGTEDTQKPENEEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N136</accession>
    <name>W5N136_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurexophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be signaling molecules that resemble neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217347.1">
        <property type="nucleotide sequence ID" value="XM_015361861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N136"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014345"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014374.1">
        <property type="protein sequence ID" value="ENSLOCP00000014345.1"/>
        <property type="gene ID" value="ENSLOCG00000011674.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683613"/>
    <dbReference type="KEGG" id="loc:102683613"/>
    <dbReference type="eggNOG" id="ENOG502QQN8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182883"/>
    <dbReference type="InParanoid" id="W5N136"/>
    <dbReference type="OMA" id="TACLVVM"/>
    <dbReference type="OrthoDB" id="8690369at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011674">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF15">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038019">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867099" description="Neurexophilin" evidence="5">
        <location>
            <begin position="23"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="267" mass="30389" checksum="21CC17EA845B6D47" modified="2014-04-16" version="1">MRSIRWLVLLLINGTACLLALGQPHASQSQKGAEGERTKSVGILSGQGLSPLGRWMLHTKSRAANTTSLELPYRSPLPFSKQEFWEMLGSDLRPDPVSRVKRRPIVKTGKFKKMFGWGDFYSNIKTVRLNLLITGKIVDHGNGTFSVYFRHNSTGQGNISVSLVPPVKAVEFDLERQSVVHPKDSKIFNCRVDYEKVDRNKKTSLCNYDPSKTCFQEQTQSHVSWICSKPFKVICIYISFYSTDYRLVQKVCPDYNYHNEMPYLPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N151</accession>
    <name>W5N151_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ALX homeobox 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015197917.1">
        <property type="nucleotide sequence ID" value="XM_015342431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N151"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014360"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014389.1">
        <property type="protein sequence ID" value="ENSLOCP00000014360.1"/>
        <property type="gene ID" value="ENSLOCG00000011685.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684196"/>
    <dbReference type="CTD" id="257"/>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160669"/>
    <dbReference type="HOGENOM" id="CLU_059011_0_0_1"/>
    <dbReference type="InParanoid" id="W5N151"/>
    <dbReference type="OMA" id="GPEADYK"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011685">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000093">
        <property type="entry name" value="ALX homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF578">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="350" mass="38714" checksum="87747028C1E4CC81" modified="2014-04-16" version="1">MTTPDNSPGLESSSGSFHPGDDLPSHPHHPHQHRLSLRGEFYSSSRVGRFCESPGEFAHGGATPNPRVSPVKLQSKYLENISPGQKGLSISGKAVFYENNQEAGEKPHPKPLGYQMLPSECRPALDDCAKMKEPPAELPGDSIADSIDLSGKNKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTELTEARVQVWFQNRRAKWRKRERYGKIQEVRNHFAATYDISILPRSEAYPQIQNNLWPGNSSGSSAGGCVLSPDSVPSSCMTPYSHSHGNIPGFMGISGSPNHHPGINSLYSLHSFPASLGSHSFDPVPMPESEYKPSGLVALRMKAKEPGSLLSWPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N179</accession>
    <name>W5N179_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Solute carrier family 16 member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N179"/>
    <dbReference type="FunCoup" id="W5N179">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014388"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014417.1">
        <property type="protein sequence ID" value="ENSLOCP00000014388.1"/>
        <property type="gene ID" value="ENSLOCG00000011704.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158411"/>
    <dbReference type="HOGENOM" id="CLU_001265_59_2_1"/>
    <dbReference type="InParanoid" id="W5N179"/>
    <dbReference type="OMA" id="YQAFSVV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011704">
        <property type="expression patterns" value="Expressed in mesonephros and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905039">
        <property type="term" value="P:carboxylic acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 16 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 16, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF14">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
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            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="426"/>
            <end position="445"/>
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            <end position="470"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="482"/>
            <end position="502"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="33"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="568" mass="61797" checksum="560301CA19894F9D" modified="2014-04-16" version="1">MAIGEKKTMVKKEEEGVVLQYVDPPDGGWGWMIVLHCFLVNILVMGTLKSFGIFFVAFQDAFGGSSEQISWIGSIMSSLRLSAGPLASVACGKLGNRLTSILGAVLVSAGFLTSILATNVIFLYISMGIIVGTGFALLYQVTSVVTAMYFRKRLATAYSIGRSGMGLTFALAPFTQLLLEQYGWEGALLILGGLMLNLVASGMLLRPISVKAPELPSSSSSLLKDHASKAEKVQGHYKNCFNGCIPLTTGSIKTDSMSGEQRNVSTPEKVPINPEASQTLEPANTLIQNGVIQNGIFQNRHIKNDCNGDNMPEETKVFLEQNGKALSIEMSTSKSVSEELPKTKILDFSLLKDPFFCIYTWSLVFSQLAYFIPYFHLSARARNLGIDPMDASFIISVAGITETVAQLVSGWVADRNLVHKYHYHKAYLILCGLVNLLSPLATTYIHLMVYAVFFAIFCGGYMALLLPVLVDLVGVEKLNNSMGFSMFFVGIGCLTGPPTAGLLYDYTQTYDCSFYLAGVCYLLSSISLFLEPLARSWKAKRWGAARSGEPECRTNGCFVSDAAKNGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1Y0</accession>
    <name>W5N1Y0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase IMAP family member 4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1Y0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014668.1">
        <property type="protein sequence ID" value="ENSLOCP00000014639.1"/>
        <property type="gene ID" value="ENSLOCG00000011904.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102687447"/>
    <dbReference type="eggNOG" id="ENOG502RB0C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="HOGENOM" id="CLU_010468_2_1_1"/>
    <dbReference type="InParanoid" id="W5N1Y0"/>
    <dbReference type="OMA" id="EMYERAH"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011904">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="3"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="199" mass="21986" checksum="AA7EB3770530F003" modified="2014-04-16" version="1">MAGRELRLVVVGKTGAGKSSSGNTILGEEVFTADISSSSVTTACERASRLVRGRRVTVVDTPGLSGRQLSGEEVQTMRRCLSLSAPGPHAFLLAVPADDRYTESRRQVAERVRELFGEDVLRYTVVLLTKADQLGDRTRGDFVQENEHLQALVQACGHRHLFFVNEDEELAPAQVTELLRIVDDMVRENRENGCEFKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2E3</accession>
    <name>W5N2E3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Prostaglandin E2 receptor EP2 subtype</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid EP2 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2E3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014802"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014831.1">
        <property type="protein sequence ID" value="ENSLOCP00000014802.1"/>
        <property type="gene ID" value="ENSLOCG00000012035.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244902"/>
    <dbReference type="HOGENOM" id="CLU_045991_0_2_1"/>
    <dbReference type="InParanoid" id="W5N2E3"/>
    <dbReference type="OMA" id="VTKKWAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012035">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071380">
        <property type="term" value="P:cellular response to prostaglandin E stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15139">
        <property type="entry name" value="7tmA_PGE2_EP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000212">
        <property type="entry name" value="Prostaglandin E2 receptor EP2 subtype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000370">
        <property type="entry name" value="Prostglndn_IP_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF8">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP2 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00856">
        <property type="entry name" value="PRSTNOIDIPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="38"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="43293" checksum="FF25F2EA82137EA2" modified="2014-04-16" version="1">SQTFTLEMGTPDGCHDRRTVEPGSPVISAVMFSAGVLGNVLALILLEIRRRKHKSRQRQSLFHVLVTGLIVTDLMGTCFISPLVLASYATNRTLVSMWRNETDLENGTSTVCNYFSFSMTFFSLATLSILFAMALERCLSIGYPYFYGRHITKRCGYISIPFIYLICIVFCLLPFMKFGKYVQYCPGTWCFINMNPESLQDKVYAILYATLMLLLVISIVTCNVSVIYHLVLMHRRHKVNRGSVEKRSRKDRRSPSMTEEIEHLILLVFITVAFVICSLPLTIRVYINSSQSITGSHRTDLIALRFLSINSIIDPWLFIILSPSVLRFLRTALCRGKACQRRNDTLKPGLEMEKHIQLSLHNTTTTNVLLKSEIHNRALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2S3</accession>
    <name>W5N2S3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein O-mannose kinase</fullName>
            <ecNumber evidence="2">2.7.1.183</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein kinase-like protein SgK196</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sugen kinase 196</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-[beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-Man]-L-Thr-[protein] + ATP = 3-O-[beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52616"/>
            <dbReference type="Rhea" id="RHEA-COMP:13308"/>
            <dbReference type="Rhea" id="RHEA-COMP:13309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136709"/>
            <dbReference type="ChEBI" id="CHEBI:136710"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.183"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.183" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01017789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2S3"/>
    <dbReference type="FunCoup" id="W5N2S3">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014932"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014961.1">
        <property type="protein sequence ID" value="ENSLOCP00000014932.1"/>
        <property type="gene ID" value="ENSLOCG00000012140.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQQV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004945"/>
    <dbReference type="HOGENOM" id="CLU_067581_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2S3"/>
    <dbReference type="OMA" id="NTWHRRL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012140">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019200">
        <property type="term" value="F:carbohydrate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036268">
        <property type="term" value="P:swimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000464">
        <property type="entry name" value="Protein O-mannose kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039318">
        <property type="entry name" value="POMK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22618">
        <property type="entry name" value="PROTEIN O-MANNOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN O-MANNOSE KINASE"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="20"/>
            <end position="39"/>
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        <location>
            <begin position="82"/>
            <end position="352"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
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            <dbReference type="ARBA" id="ARBA00011932"/>
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            <dbReference type="ARBA" id="ARBA00015906"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00025665"/>
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            <dbReference type="ARBA" id="ARBA00029343"/>
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            <dbReference type="ARBA" id="ARBA00030304"/>
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            <dbReference type="ARBA" id="ARBA00030430"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014932.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="352" mass="39396" checksum="AA931EE904977B79" modified="2014-04-16" version="1">PAQEDETMVKGGGSHGGVPAVLICLGALLCANVLIYLYLDSLYQGSNLHEHLYGDCPPGHFRIGAMRNCSPWLQCEAVLKEVRKLKMISQGAVKQVFLSEWKGHKVALSKLSSLDYREDFLHGLEMLKALQSPYVVTLVGFCLDNHAIVTEYQPLGSLLNLNAVLNLEKYSNVNTWQNRFRLAIEYVEIQAFLHNSPVGVRVMCDSNDLGKTLSQYLLTTDFHLVANDLDALPLVDHDANILAKCGPRELLGEFVAPEQLWPHGEELQFTDGLMPGYDEKTDIWKIPDVVRFLLGQVEGSDVVHFHLFDTHAACKNRDPSQRPSAQEVLKVYRTVFAAMIKDSPTPHSRDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3J2</accession>
    <name>W5N3J2_LEPOC</name>
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        <recommendedName>
            <fullName evidence="3">Nucleolar protein 16</fullName>
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    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="180" mass="20955" checksum="B0D99F7E79F410C8" modified="2014-04-16" version="1">MPKAKKSSRRKKFDYTQNRKKLKLKFKKAAAPRIECAQIRNAWDDKKSVTQNLKEMGLAFDPNRSLPIQKAKVTAVEAGVSEAHSTTVKKPYVLNELAAEASLPSKNTRTLSSDMIEYVQYMIQQHNDNYKEMAKDEKNYYQDTPKQIKRKVELYRRYHPEEYAAFMDSLSDRNQDIVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4G7</accession>
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        <submittedName>
            <fullName evidence="6">RPTOR independent companion of MTOR complex 2</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG2"/>
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        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
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        <location>
            <begin position="58"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="524"/>
            <end position="744"/>
        </location>
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        <location>
            <begin position="922"/>
            <end position="994"/>
        </location>
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        <location>
            <begin position="1023"/>
            <end position="1069"/>
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        <location>
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        <location>
            <begin position="1160"/>
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            <end position="1301"/>
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        <location>
            <begin position="1023"/>
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        <location>
            <begin position="1127"/>
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        </location>
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        <location>
            <begin position="1191"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1221"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM01307"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1705" mass="191243" checksum="D61CDEDC7D0E0CE8" modified="2014-04-16" version="1">NMAVSIRGRPIRSLRMRGRNDSGEENVPLDLTREPSDNLREILQNVAKPQGVTNMRKLGHLNNFIKLLCNVGHCEEKFGFTYEEIIICLRLALLNEAKEVRAAGLRALRYLIRDSSILQKVLRLQVDYLISRCIDIQQSNEVERTQALRLVRKMITVNALLFPSSITNSLIAVGNDGLQERDRMVRACIAIICELALRNPEVVASRGGLSTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRSDVELEQILAPYTDFHYRHNPDTAEGQLKEDREARFLASKMAIVAAFRSWSGIINLCKSGSSGIQSLIGLLCIPNMEVRRGLLEVMYDIFRLPVPVVTNEFIEALLSVDPSRFQDTWRLSDGFVAAEAKIILPHRARSRPDLMDNYLALVLSAFISSGLLEGLVEVITSSDDHVSVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDISQEKRMRASAAVNCLKRFHEKKKRGPKPHSLYLDHIIRKSAAAHYRRDQHLRVQKDIFILKDTEEALMMNLRDSQILNHKQNLDWNWVLIGTILKIWRMVKLQPLCQEQLSQFVRRLLYFYKPSSKLYASLELDHSKAKQLTVVGCQFIEFLLESDEDGQPYLEDLVKDIVQWLTSSSGMKPDRSLQNNGLLNTLSQHYFLFLGSLSSHPYGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSTLDYSRDGLARVILSKILTAATDSCRLYATKHLRVLLRANIEFFSNWGIELLVTQLHDKNKTISMEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVTKQLEKWQKEYNLKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPNVYLPVHLYGQLVHDKTGCHLLEAQSIVPDLSYTVRSPMLDKWEGVKHLKAALWALGNIGTSNWGLNLLQEENVVPDIIALAQNCEVLSIRGTCLYVLGLMAKTKQGCDILKQHRWDAVRHSRRQLWPVVPDDVETLCSELPSVPSTLSLNSESTSSRHNSESESTQPSMYILDDDRCDGTGEDEDQPLFMRPKTLKDRSRLTILASSRFVRNRFINSLTLPSKKLRSSSDPKGGKTPTEPKPGLRRNRTVTEPSSYSQGDVFTPVFNGNRFSKSPTVSLETSFVGSKASEDQGSTPSIGEPELKLPRVLGGGGENHREHASRERLVGDGTAGAQFKSRSQSFNTDTTTSGISSMSSSPSRETVSGVESGTIDTDCVSLNTVVSTKTVKTLHSLTPQANHLPLSKSNSVSLVPPGSSHTLPRRAQSLKATSVTTIKSLADYNFMYTSPRDAFGYATLKRLQQQRIHPSLSHSEALASPAKDVLFTDTITMKTRSLDSRLTPRRFLKALSFASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGCSDDYIGLALPMDINEMFQIKETPYFQQKTSPPPADEHLAKFVFSESDGPGESGRNPLQALRQQISITELMNTSRSDQAQLLSNSEDTGLQEHTDNNCLYCVGVQVLGCQAHNQINSTLNRTEFNDIPYSDWCGQTIHNHLEVTPQSKFSGVSGCSDAVSQGSASSTRSTELVLGGKAISEDTPACRVLLRKEVLRLVINLSSSVGTKGHETGLLTIKEKFPYAFDDICLYSEVSHLLAHCMFRLPSRRFIQELFQDVQFTPMYEEAEAILATPPKQVSADLQPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4J1</accession>
    <name>W5N4J1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Reticulophagy regulator 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RETREG family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4J1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015550"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015579.1">
        <property type="protein sequence ID" value="ENSLOCP00000015550.1"/>
        <property type="gene ID" value="ENSLOCG00000012630.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683050"/>
    <dbReference type="KEGG" id="loc:102683050"/>
    <dbReference type="CTD" id="54463"/>
    <dbReference type="eggNOG" id="ENOG502QPW8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166973"/>
    <dbReference type="HOGENOM" id="CLU_036265_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4J1"/>
    <dbReference type="OMA" id="IKGAQLW"/>
    <dbReference type="OrthoDB" id="10029527at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012630">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057282">
        <property type="entry name" value="RETREG1-3-like_RHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043384">
        <property type="entry name" value="RETREG1/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659:SF3">
        <property type="entry name" value="RETICULOPHAGY REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="11">
        <location>
            <begin position="56"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="450" mass="49242" checksum="7194F32BF5ABAD9F" modified="2014-04-16" version="1">MASLGRGSGEDAEAGGQDGKASTESLRITEAGEMADAEMKEQVAAPRQPRDSSLFSHFVDVVTWRRPLSSTLLFAATNAVFWFVALSSWRVCYLLALSLVCLVTVQMVKDLVLSRTRGAQLWRRLSESWEVIDPKQESRPEAGQLTECWMSYSLFLQEMSSFKQQNPGKFCLLICGLCTFLAVLGRYIPGVVISYFIVLCVFLWPLLSSHEFGQWIEPVLQKLDFGVGGFLQKMKENHEKRNAKRGEENSEAELSALFPKLDSSTMCKELSISDTEASEVSWTDNGTFNLSEGHTPQTETSDDLDRPSDHEEAFAGGLSEFPSVDNGTNGDDEDLSIGLPTPSFGQTEGDRPSAGERALDLVNRMAGDVIAAAVTAAVREQLGAPSWSAAPPALDFGGETDSEDGEDFELLDQSELEQLQSELGLAQEAQEAEQKSSKPSGFLSNLLGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4T3</accession>
    <name>W5N4T3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01000356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4T3"/>
    <dbReference type="FunCoup" id="W5N4T3">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015642"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015671.1">
        <property type="protein sequence ID" value="ENSLOCP00000015642.1"/>
        <property type="gene ID" value="ENSLOCG00000012710.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689678"/>
    <dbReference type="KEGG" id="loc:102689678"/>
    <dbReference type="CTD" id="57602"/>
    <dbReference type="eggNOG" id="KOG1865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157948"/>
    <dbReference type="InParanoid" id="W5N4T3"/>
    <dbReference type="OMA" id="RSAMCDT"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012710">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904888">
        <property type="term" value="P:cranial skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF653">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="122"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="694"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="775"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="866"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1127" mass="122601" checksum="9135C88989DBDBE6" modified="2014-04-16" version="1">MPIVDKLKEALKPGRKDSADDSDLNKLLASSAKKVLLQKIEFEPASKGFSYQLEALKTKYVILNPKAEGAVGQRAADPLPMKKQVSEGVSGGQSDGIPAPQKMLFPGNRLSMKWERIYRVGAGLHNLGNTCFLNSTVQCLTYTPPLANYLLSKEHSRACHQSGFCMMCVMQNHIIQAFANTGNAIKPVSFIRDLKKIARHFRFGSQEDAHEFLRYTIDAMQKACLSGYTKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIAVEIRQAANIVRALELFVKPDVLSGENAYMCAKCKKKVPATKRFTVHRPSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSMVHSSNIKVVLNQQAYVLFYLRIPETRKNLDGPISKQGVVPSGRNSVTPEQLKKASLNGPLSSPQVGKKLESSQVRKVQAADGVGVPVPRNGSACQSPKLANGSPLLPAASRAPAGPTVIEEPLKKVKKPSPQLQARSGPSGAPNGFGGAPKPESDRRHAAESRGLSASTSMKSLTDSSPDTSEAKDGEPSGLPAPPKDASSPRGLPKGEPPARSGLSGGMASPRRGPEAPSPGGPEERSVKFKPQALTNVASEPASTISPPPAKKLALSAKKARSSQSPSGSEASFSPRHLPSDSTHPTTSRHFSSPTAAHRHRADSFHSPKLQALPKPWSDSLCSKDGHRKSAIASPFDSVGMQLPCGSPSQTNGFLPKSPEKGSSQSSPVDTTEPSYVPGPAKKPKKHKRRCPGGDDRPPSVAQNGHKEGAEEAPKKKKKRRKRRAEDEEQGVAGPARESVLSHLESPRDPPRGPGSPPRLPAPPESWTASAEPPTLRPPGEEAAPGDKKKKKRRKEKSKEGTSGKESLSKSVCDSSGIREAAVGTDADDLDKLKKKREKLKEKAERRKRKRSASDEEGRSSDPLPEESTVNGGRAGKTKPVVVWDGQVRDGYKRTPELPAAQSSSAWDGRRSCSVVTELLRHSEDRAYGAEVLTWDGEVSALSREAIEDAHIARNDTVIDEWDEDFDSGKVKKVKKYRKEKRRNFNVFQKVQNRRNLWSVTHGGRMTSLGHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5Y1</accession>
    <name>W5N5Y1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GA binding protein transcription factor subunit beta 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01026083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026084">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5Y1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016040"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016070.1">
        <property type="protein sequence ID" value="ENSLOCP00000016040.1"/>
        <property type="gene ID" value="ENSLOCG00000013022.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRTX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157875"/>
    <dbReference type="HOGENOM" id="CLU_000134_12_0_1"/>
    <dbReference type="InParanoid" id="W5N5Y1"/>
    <dbReference type="OMA" id="PXEREAL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013022">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000025">
        <property type="entry name" value="GA-binding protein subunit beta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050663">
        <property type="entry name" value="Ankyrin-SOCS_Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24193:SF76">
        <property type="entry name" value="GA-BINDING PROTEIN SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="356"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="44236" checksum="B59DBC7DC29FC8EC" modified="2014-04-16" version="1">MTSRQQAEFQNAQALLTLMSLVDLGKKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLSAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHARIVEVLLKHGADVNAKDMLKMTALHWATEHGHRDVVELLIKYGADVHAQSKFCKNALDIAVDNANEELAEILQVSMQNQINTNPESPDTVTIHTATPQFIIGPGGVVNLAGLVSAANSSKSTDEAGVSTVQFGNSSTSVLATLAALAEASAPLSNSSETPVVATEEVVTADSVDGAIQQVVSSGGQQVITIVTDGIQLGNLQTASGIGQPIIVTMPDGQQVLTVPATDIAEETVISEEPSVKRQRIEIIENHVENTGIEEKEALQKQLEEANREAQKYRQQLLKKEKEAEAYRQKLEAITRHQSHKKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N748</accession>
    <name>W5N748_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Papilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the papilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031876">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016457"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016487.1">
        <property type="protein sequence ID" value="ENSLOCP00000016457.1"/>
        <property type="gene ID" value="ENSLOCG00000013346.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156891"/>
    <dbReference type="HOGENOM" id="CLU_000660_7_0_1"/>
    <dbReference type="InParanoid" id="W5N748"/>
    <dbReference type="OMA" id="KLDSGWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013346">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22635">
        <property type="entry name" value="Kunitz_papilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000017">
        <property type="entry name" value="papilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868921" description="Papilin" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="767"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="917"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="1072"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="1166"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="16">
        <location>
            <begin position="1265"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="844"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="963"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00061693"/>
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            <dbReference type="ARBA" id="ARBA00074534"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1314" mass="143313" checksum="A5FC4A6D3F1D68E7" modified="2014-04-16" version="1">MLALLLLAVLHLILTPALCRERRQSDYWDEWEDWGECSRTCGGGITVRYRRCISQRRDGGSSCVGPDRAFRSCNIQDCPEGSRDFREEQCSQFDGADFQGKRYKWLPYYGAENNCELSCIPKGENFYYRHREAVVDGTPCHPGRRDVCVEGVCKPVGCDNMLESTQKEDPCLECGGTGKSCYPVRKTFTVLDLPKGYNQMFIIPVGATSILIREMAPTRNFLAIKNVRGEYYLNGHWVIDHSRAVHIASTVLHYERGAEGDLAPETIRGRGPTTEPLVIELISQEQNQGIEYEYYVPYRSSNEGYFWSYGSWSECSKECGSGYQSRLVFCIIDNEAYQDYLCSGRPRPASNRTCNPQPCPQTRRWKTGEWSACSATCDGGTQSRSVSCLIHDGSGSRVVEDAQCAAFSSRPSSQQACSLRQCASWSARSWSQCSVSCGEGEQTRPVVCMGTAGIRLPDYACSSLPKPETVRTCQRQGCPREIRWHIGDWGLCSKSCDVGLRERQVICADRDRNFYNGELCAADHKPSTTEQCNTQACFLPQSVPSMPDPRGYDNTRLGILVPYISEEPPTECLSGILPVLKAGLCPTKHLDIICENLLNLLFPRGGTDNRRSDTLYDRWVSQPQSCSESQYGCCPDGRTPASGPLGSGCPIHSCAETRYGCCPDGVTAAQGPDGAGCQTNTVRATHHTHYTGALCDRSAGCALVAERELFVSPLSRQQHHPAAPRREPSEACRSANYGCCYDGLTLAAGPNGEGCPGQPNHSHPLACMLPNTNGPCSDWTARWFYDPSSGGCGRFWYGGCQGNRNNFATEEECLRECRGLATQSRAYGDRHHFRLTTLAERGPAVSLENSRAQGGVRLQGSVQREDRGTLTATDTSPAKGPARLPEEGTTSTAPGHPEEERGGGSDGGDGPPRPARPAFQLPVGLVDSRTLQRLSIDKSGPSSVEGQMGQTVRLPCKVTPHSSGVTVEWRKDSQPISTPSRHRQQADGSLLVGPLSSQDTGWLLCVASSDRERDHLYIYLSVSEDARSAPASQNPSVQQGSGQVSGSRVHRYCPSHTLLQRTAPLICPSAVPIIMLLSIDKSGPSSVEGRPGQTVRLPCRVSPTSVSVEWRRNDRPLTSARHTQQSDGSLVISRLSSEDSGFYTCTASNGQQRVHRQLQLRVLGDLRITTAPNNVQVSQGSSTQLPCVVSGSDVNVGWSRNGVPVRPDGRHVLLSADGSLILNNVQPSDEGSYTCNAYTGTYSVSATAEVKVAKGTELALPSRPNTAECIDQPDLANCDLIVYAKLCSSEYYSSFCCASCSSHSQPAGRHAEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7M0</accession>
    <name>W5N7M0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Si:dkey-49n23.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015167">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7M0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016629"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016659.1">
        <property type="protein sequence ID" value="ENSLOCP00000016629.1"/>
        <property type="gene ID" value="ENSLOCG00000013481.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3611">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165085"/>
    <dbReference type="HOGENOM" id="CLU_009051_5_0_1"/>
    <dbReference type="InParanoid" id="W5N7M0"/>
    <dbReference type="OMA" id="HLMWEPV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013481">
        <property type="expression patterns" value="Expressed in liver and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF69">
        <property type="entry name" value="SEMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="12">
        <location>
            <begin position="1"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="530"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="637"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="724" mass="82565" checksum="58DDD2A59496E287" modified="2014-04-16" version="1">DLQTSNNSAAFWAYRESSSYQALLLDEDRGWLLVGAKDHIFMLRSDNLNEPARKIYWPAAKEHIEHCKLAGKNAETECSNHVRLLQPFNKTHVYTCGTGAFHPLCTYIDLGHNLEEPTFRLLRHAVESGRGKCPYSPLEPFTAKLTDGELYAGTSVDFMGTNAAIFRSPVQGISQHYIRTEANQDQWLNEPEFIGSFSIPDTHSPDDDKVYFFFKETAVESIQWDKRIYTRVARVCKNDVGGKRSLINRWTTFLKARLVCSVPGPDGMDTYFDELEDIFLLETKDSQNPLIYGVFSTSSAVFRGSAVCVYSMASIRAAFSGPFSHKEGPDYRWVEYKGRIPYPRPGTCPSETYDPLHKSTRDYPDDVISFMRGHHLMWEPVLPLNRRPIFMRTGAPYLIKKVVVDRVEAEDGQYDVLYLGTDNGKVLKIIAIPKDNWETEEIVLEELGLFQKPTSVLSMVLSTKWQQLYVSSQSGVTQVSLHRCDLYGTVCADCCLARDPYCAWDGTSCSRYFPTNKRRARRQDVKYGDPKSQCHDLGDGLDAVEDKVIYGVETSSTFLECVPKSQQTSIRWTVQHHPGKHSKELKSVERFVTMERGILIQRLEQGDAGLYSCLAEEHAFSHPITHYTLHVITQQQITAHPGKDRSGEPGLGSTPMRHSSSAIEQIQRHYKDYVQLMSVSGLSADEYCEQLWFREKRKQKMRNLKWKQAVENKKGRVRRHPASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7N2</accession>
    <name>W5N7N2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01024878">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024879">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7N2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016641"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016671.1">
        <property type="protein sequence ID" value="ENSLOCP00000016641.1"/>
        <property type="gene ID" value="ENSLOCG00000013489.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158905"/>
    <dbReference type="InParanoid" id="W5N7N2"/>
    <dbReference type="OMA" id="GWGMQIA"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013489">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010766">
        <property type="term" value="P:negative regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032801">
        <property type="term" value="P:receptor catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000006">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000008">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000047">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF282">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 15 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="20">
        <location>
            <begin position="189"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="20">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="20">
        <location>
            <begin position="430"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="20">
        <location>
            <begin position="481"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="21">
        <location>
            <begin position="573"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="311"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="16 17">
        <location>
            <position position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="908" mass="105355" checksum="444A700A9F60ED01" modified="2014-04-16" version="1">TMASVAEVRGLQTDEEDSRILRVKVIAGIGLAKKDILGASDPYTRVSLYDPVNGELTSVQTKTIKKTLHPKWNEEFFFRVHPRKHRLLFEVFDENRLTRDDFLGQVDVPLHQIPMENPNIERPFTFKDFLLHPRSHKSRVKGHLRLKMTYLPKSNGSEEDNAEQTEELDPGWEFLDQQDMAGTCQQQLPALPPGWEERQDNLGRTYYVNHDTRTTQWHRPTIQDSQAEEEQRQTIHLEAQHAFTARRQISDHVEGHDRRDSPDSWEIITEDEATIYNNHNQRSPPPPLSPMEQPLCDVQSHLADELGRSLQTSAQVAGDRRMSLPHQSHSSRRGSSQTPTLEEQPTQPVFMPTSAGLPPGWEEKQDSKGRTYYVNHNSRTTTWTRPVIQTTTEAVQATSCTSPTVCQPPLLSPETSPQHASSQKPLYETGFLPAGWEIRSAPNGRPFFIDHNTKTTTWEDPRLKIPVQMRRRLSLDPADLGPLPPGWEERVHTDGRIFYIDHNTKATQWEDPRLLNSAITGPAVPYSRDYKQKYEYFRKKLKKPADIPNRFEMKLRRAAVLEDSYRRILSVKRADFLKARLWIEFENEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLQDMESVDSEYFNSLKWILENDPTDLDLRFTIDEELFGQIQYYELCSGGTDIVVTNKNKKEYIHLVMQWRFVNRVQKQMSAFKEGFYELIPLDLIKIFDENELELLMCGLGDVDVNDWRENTKYKNGYCATQPVIQWFWKTVLLMDAEKRIRLLQFVTGTSRLPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPPYESFEELREKLHLAIENAQGFDGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7U7</accession>
    <name>W5N7U7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prostaglandin reductase 2</fullName>
            <ecNumber evidence="4">1.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">15-oxoprostaglandin 13-reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133374"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:50590"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133408"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:50586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:11918"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>13,14-dihydro-15-oxo-prostaglandin E2 + NADP(+) = 15-oxoprostaglandin E2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57402"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:11914"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15-oxoprostaglandin F1alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79072"/>
            <dbReference type="ChEBI" id="CHEBI:133411"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:50594"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADP-dependent oxidoreductase L4BD family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01031839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7U7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016706"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016736.1">
        <property type="protein sequence ID" value="ENSLOCP00000016706.1"/>
        <property type="gene ID" value="ENSLOCG00000013550.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156793"/>
    <dbReference type="HOGENOM" id="CLU_026673_29_2_1"/>
    <dbReference type="InParanoid" id="W5N7U7"/>
    <dbReference type="OMA" id="EEKCRYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013550">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08293">
        <property type="entry name" value="PTGR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037399">
        <property type="entry name" value="PTGR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF5">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="17">
        <location>
            <begin position="36"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="39254" checksum="5BC8D95302E59A79" modified="2014-04-16" version="1">IYLTHARFIGLKVNTNMIRKVVLNSRPGNNGQPVPGNFRVEEGDLPSDLKPGQVRVRTLFLSVDPYMRCRMNEDTGTDYLLPWQLSECVDGGGVGVVEDSKHAALSKGDTVTSFNWRWQTEDVVDGAGLQKLDPQLVDGHLSYFLGAVGMPGLTALLGVREKGHVTAGAGQTIVISGAAGACGSLAGQIGRLDGCSRVVGVCGSDEKCGILVSELGFTSAINYKREDVASRLRECCPKGVDVYFDNVGGPISDTVIAQMNEDSHVILCGQISQYNKDVPYPPPLTASTQELLFRKNITRERFTVLNYVEKYEEGLMQLSQWIKTGKLKALETVVQGIETMGETFCSMMEGGNIGKQIVQISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8B3</accession>
    <name>W5N8B3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein protein MPP10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the MPP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8B3"/>
    <dbReference type="FunCoup" id="W5N8B3">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016872"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016902.1">
        <property type="protein sequence ID" value="ENSLOCP00000016872.1"/>
        <property type="gene ID" value="ENSLOCG00000013681.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689918"/>
    <dbReference type="KEGG" id="loc:102689918"/>
    <dbReference type="CTD" id="10199"/>
    <dbReference type="eggNOG" id="KOG2600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011359"/>
    <dbReference type="HOGENOM" id="CLU_011271_3_1_1"/>
    <dbReference type="InParanoid" id="W5N8B3"/>
    <dbReference type="OMA" id="HFAEDFG"/>
    <dbReference type="OrthoDB" id="445326at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013681">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
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        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
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        <property type="entry name" value="snoRNP_Mpp10"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
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    <sequence length="691" mass="78325" checksum="4A4CA468DABEFA01" modified="2014-04-16" version="1">MADRELGFTLESCLNILNTNTAHPEKFLSVQDVLASDFTSLTKTLYDLHKAQEPPGAVGSPLKELVVENFDEEQIWQELELQNTAALAQFQKAVGLALRDKEIGFDEAEEEDREDGGSEEESAAELSGELGGEDSGEDGSVQEEEEEEEEERRASKPRKASAKSEALENFSDEDSDVDFDVDALERQTKRASERTRRPGGGVGSVVDDRFFKLADMEAFLEDVENKEQQGDEGDGEIDYFEDILSEEEEELGLEQKTAGRKKGKQQKSSRDLKYKDFFDPIEADVGQKGPNEDDEDLGSEEEIDEEMEDEEDMEEEEDMDEIEDNEESRQAKEALKRVTFNLSDDSEGEEVESILGGKQPSSEKAEPKSSFEKRQEKMAQRIRELEKAALEEKPWQLTGEVSAQKRPENSLLEEDLMFEQAARMAPVITEETTLLLDDIIKQRIKDQAWDDVIRKEKPKEDAFEYKKRLTLDHEKSKLSLAEVYEQEYLKQTQQKKEEEENPAHAEIQKLMDSLFLKLDGLSNFRFTPRPPVPEVKIVSNLPSIAMEEVAPVSVSDATLLAPEEIKEKNKAGDVVGESERTATDRKRERRKKKLLKRIRLKKKEKWQKPGEQAQAGDKKLSKAQATEQLKKVTKGGKAALLKDEGKDKALRSSQAFFSELQDQVKMQIKGAKNQVAKKSKRKEVSASKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8P1</accession>
    <name>W5N8P1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Si:dkey-11m19.5</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000017000"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017030.1">
        <property type="protein sequence ID" value="ENSLOCP00000017000.1"/>
        <property type="gene ID" value="ENSLOCG00000013786.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013786">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
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        <property type="term" value="C:inner dynein arm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
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        <property type="term" value="F:dynein light intermediate chain binding"/>
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    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
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    </dbReference>
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        <property type="term" value="P:flagellated sperm motility"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="AAA_6"/>
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        <property type="entry name" value="AAA_9"/>
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        <property type="entry name" value="DHC"/>
    </dbReference>
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        <property type="entry name" value="DYNC2H1-like_lid"/>
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        <property type="entry name" value="Dynein_2_N"/>
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        <property type="entry name" value="Dynein_AAA1S"/>
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        <property type="entry name" value="Dynein_AAA5_ext"/>
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        <property type="entry name" value="Dynein_HC_stalk"/>
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        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
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        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
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        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
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        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10676:SF359">
        <property type="entry name" value="DYNEIN HEAVY CHAIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_6"/>
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        <property type="entry name" value="DHC_N2"/>
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    </dbReference>
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        <property type="entry name" value="DYN_lid"/>
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        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Dynein_heavy"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="14">
        <location>
            <begin position="104"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="15">
        <location>
            <begin position="722"/>
            <end position="1078"/>
        </location>
    </feature>
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        <location>
            <begin position="1298"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein 2 heavy chain 1 cytoplasmic ATPase lid" evidence="20">
        <location>
            <begin position="1606"/>
            <end position="1703"/>
        </location>
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            <begin position="1837"/>
            <end position="2079"/>
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            <begin position="2192"/>
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    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="18">
        <location>
            <begin position="2623"/>
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        </location>
    </feature>
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            <begin position="3054"/>
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        <location>
            <begin position="1721"/>
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        </location>
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        <location>
            <begin position="2139"/>
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        </location>
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        <location>
            <begin position="2339"/>
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        </location>
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        <location>
            <begin position="1721"/>
            <end position="1732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3309" mass="374934" checksum="FA5AD722644F6982" modified="2014-04-16" version="1">MLDLWDSFVPLLQVATETVSTRLPSMVETLNSIYTSLSEELQGLVSTATTGQYLQPSQCAAVVLEELKGIAQQFQTVAGKLYDLNRTSQSLTGSPLDLSFIPSTERKLEARKGVWELLSQATMQINEWKRILFSKFVVAHAQEKVNDWLNQASLLVQTLPAQDNVFKETMQLIENFSQQLPLLLKLSSPTLKHKHWRHIFKGLGLMYETEWHLTVADLLPKSLLEYQNSITKVWQDAKAEADMEQAFRKLQRRWNVEHFRLANFIVKVCTWTGIWCSKINYLFDWLILGICSPFLCDCFENLLALAEDSVMTLSNMMLSPHISEFRQEVENWVHLLQELEELLGFWQRYQQEWVFLSKIFYETEVSIQKPELFCRFRTVDSNYRDMMQATSCSPRVLNIVRHGKKTGTPGQFQGQCLRSVFMEELSTMEKISNQLLFLLDSPRCEFPRLNFLSDGDVIKLLSLHPNHSALLPLVRKCFPSVKELVLKTLDQQHHTMTSMDLSDLQMSVIGVNGNLGEKLPFSSPLPSNYNLLAWLSDLEQRMREAVFRLLWDCIVARMTPAPVTQTETWNQYKRSSDSDEFNDPSWDLTSQFPLQCLLVAEEVLWCKEIQSSFLNQTVVKRTRLKARYATKLKGLIQIIQEHSLRYGESLDSQRVMSAFKALVLVAMKHCDLMSRFLEQKTEVESSFEWQKLLKYSLNSDPKHSGLHAKLCYVDVLGSHIPYGYEYVGPDDWMIVNTPSSDRAVLGILLSLTSYRSSLVIGPSMSGKSKTVVHLGKVLGQQVVTLQYTREMSPSIVLQMLSGAIQTGAWLVLECVNVMTQGDLSLLGQYFSDIQRALTALQKNQQLRISQTKPYEPQSFGLISFAGRTCLAKHGYGCVLIMSGSTSEIPENLRVATRPISLIQPDYLIIAEVSLAALGFSEAQSLAHRLVCLLTLAQKSLCLPQFINNCTSWLVVLKNIITTAGIHLHEHHLKTLLLEAQDEEQAILKAISLVVVTAILDPEKVSHFQSLLEDIFSASPLQGTETDEIAILSSAVTQELQEIGLHSDQWTIKNVLSLYEALKLSRAVILVGPAGSGKTACYRTLVGALKSVTLKSKWSSVDTMVIFPNSVSEEEFSGICNHDEQHGGWWRAFTKILKDSERRDFSTRERQKSQTHKGKWVVLDGEPLAEGWMDQLSTLFDLEDPYLCFSSGQKVRPSPEQVKLLMEVTELASASPSTVTRCGIVYHSGKEVWKAIWKAELETLYREFSLDQTTLRLWNRLAEDLFSSTLSFLKHKALSPVLLEGSILSADKTTAHGVQEVMSFTRILHSFLEQYLIGKEAESPSTTKLEDPGNLLSQKHLQAKNIFVVSYVWGFGGHLHPKYWLRFDAFARKSLYESWYSIEIPQEGTVYDYLINQNEGTLEMMNTGLLNSRFEKYAYLLDVLLDSRQPALLVGETGAGKTTLSQSLLNYERPHVRLPITPCLRAADVRNVLENMWSPRAKLSAAGLSPAGSKQSCLFIDDLHHTTCDVGSKISSVLETLRQCMSKGGVLTSDGYHFKLLNSGAVSYLATCVSPECCSSMTICISPRFFRLFSVLTLPSLVTEHLLSIHSPRVLHWLKDLHITAVSQYTELASCIIGATVDLYSAVKENFPPHQQQPYLLFSYHDLQKVFQGMFLWTPRTTARQYPQISSTHGSLAAVLNITRLWIHECFRTFGDRLSSENEKRVLASLLNKVSQKYFCPKESDSQKTKGENESCAMPSENNQNSEKSEIILPLPDKDDQSEDIISSDSETCQDIIFSTNFCESLQSPTQQYSLKRSSVYQEKDMDLLIQQLSSLLQWQCREEMIDKGFVSGPESKLAIFRERVQQLTHILRVLLLPRGHAALFGMGKGTGRKTLVRFAVSFSGYRLYDVNPNNETEVSEILKEASDHAGVFGGRVVILVHEEVSQSVLDKLLAVMAEGTFPGLYNKDELSAAVQKITALTKNTKNRLGEEQVLERYYKHVQKNLHVFIMLSPKCQKHAEPLKEKMLSEIFLSRLLKLCSCVEMFQPLSAETLSEVAASRLQDVILPPEEEGKGEEACSLEVLTKSMATIHQSVLRYVTHQVPGLHLFSLQVFLEFTEHFRDIFTHLWQQARAQANRVATILRRLKILTNTAKQSSQEVLFVRNELKVAQELQQQLQREVDAQRILCEQTRQRCLQEENILSDLSEQLQQAQHESQEAFQQAALDALHSLTVADIEEIRCYRVPPDGVVMVMNAVCLMFGRPRNWESSKQLIGQTSFFQDLEFYDKSGITDSLFTALGRIVVQPEFQPAVVREASRACESLCLWLHSIYQYASVQRHLAPQHNRVHQVEEHMVKSRIRLGQERVQEEAARECLEELEKQYQGASQHVGELTLQLRHADAREREAHSAAQLMHSHITNWTAAAKKATEDNHNAVGDALVLAAAASYLGPFNPDVRLEMLEKWQELCHTGEMQLNPRDQRHEVLPTTVPPVPRNIVLIPVSKELPLSKALGGCVSASETFSHRPVLQLVIRRSRCPFVQRWPLLVGSAQQAKSCAEAILEEHLSHLDLGNAHTVYSFMLRKLSQTQSMAHPWTLYNSLRMCLALGVMILVIAKATFKECFRILLFAEDENLYLPESSSQPDTWMVLCADDPTLVEKIQKGAERGLVVLVTDVERVVLSPKLLDFLQRKPEKWASGLRTDSMAAHHHFTLFLSTHLPVRALAQELDSNILKVVNVIDISLSQSELEELLLKELVLSECPEVWGQRRTVQMEKLQIVNRMQEVEDSLVEYILQSLTPLLEDPCFLSHVSECQKTMSVLHQDLKEVSVELQHHCVLLKDFDGVVKLGSALYQALQEVSRLSPLYFFPLQPFLQLFKVALGHNGRTEGANGGEFTSATMAEITHRIVSDVLSHYRPCLFQSHARFFQLLVSVALIRHNDECTSLEQQIFLKGLKDVVSSLDLHSTSTHLVKWVDEHTMREICYLENLTAFKGLSSSLLHLSEQWQEYFKFPSSTVIGPVPCSSYAQLSTLQKAVLWKTLHPEWLFAVAEDLAYTQLGQTLASVDYATTEISSQLLNLPAIPVIFLLPQKGDLGPSTHPLYWIEQAVKTQANQVRIHVISFGSHCQRESTLHTLDTCTRGGHWLVFNNCHLLDQWDSELVSQLSKLFSQTARGRRNSISVSICRPASCGERPIHPQFRLWFITRSDAPQSIPGVQEQHVTDLNCIKTTVQCGQVEKVSLRQAAAGPCPPADLQIRCAVLHSVLLQRQNYRQIAQGHLHCWTQEDLLAVLNAWEKIERLCEDPVQALEFIAGRNVYQRLRKLNLVCQVDKYSFTRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8T7</accession>
    <name>W5N8T7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Toll-like receptor 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002170">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8T7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017046"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017076.1">
        <property type="protein sequence ID" value="ENSLOCP00000017046.1"/>
        <property type="gene ID" value="ENSLOCG00000013826.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166529"/>
    <dbReference type="HOGENOM" id="CLU_006000_4_1_1"/>
    <dbReference type="InParanoid" id="W5N8T7"/>
    <dbReference type="OMA" id="PYIYFWG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013826">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090594">
        <property type="term" value="P:inflammatory response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032728">
        <property type="term" value="P:positive regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000008">
        <property type="entry name" value="Toll-like receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000137">
        <property type="entry name" value="Toll-like receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041015">
        <property type="entry name" value="TLR3_TMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF524">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17968">
        <property type="entry name" value="Tlr3_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="22" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867419" description="Toll-like receptor 3" evidence="24">
        <location>
            <begin position="26"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="25">
        <location>
            <begin position="755"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="903" mass="102822" checksum="08F2C27764D8E133" modified="2014-04-16" version="1">HGRGKMILFLVIVEVAFFTPEVCHADQKKTHCEIKNRKADCTHLKLQNVPSYLPENITALDLSHNQLKTLPSSALSRYHQLYYLDAGFNSIQAIDPQLCLTLPQLQVLKLYHNEVHVLTEAMLQNCTHLTELYLASNRLKLKGEPFKCLQSLKRLDVSRNDLTSAKLGTHPQLLNLEELILSGNAISVLKADDFSYLSNASLKILKLSALPLKKFEPGCFQCIGRLSVLVMDGSKLGHPLMAKLCSELSETKIRSLSLQDTSLLTLLNTTFKGLQSTNLTSMDLSKNEIPAVQRSSFQWLHSLENLSLAENNFKRFTKDMFIGLGSLKFLNLTKSLKKHKSSSYPVIDDFAFQNLLNLEFLAMESNAFREITGNTFTGLKSLKYLSLSHSYFNLKTVTNVTFSSLAQSPLKILDLTKTGISYLDVGAFFWLRNLSKVYMGQNKISQVLGGREFEGLNNIEEIFLSYNKKITLTPSSFNYTRTLRTLMLGSNLIDSLDLHPCPFQPLGNLTVLDLSNNNLANINAGLFSGLHDLKILKLQHNNLARLWKKANPGGPVLFLKDVPQLEVLQLDSNGFDEIPVDGFKGLFHLLELDLGLNYLNVLPDNVFNDLTSVRVLKLQKNFITSVKETVFGPVFRNLSVLHIEKNPFDCTCDSVLWFVNWLNVTNASVPQLSSQYVCNTPPKYYNSSLVLFDTSPCQDQAPFKPMFVTTSSFLLTFMAMALLIHFQGWRIQFFWNVLGNRILGYKEVDLGENRFEYDAYIVHAQEDWEWVERNLTPLEENPFTFCFEDRDFLPGTPHLENIVETIRQCRKIVFVVTEALLKDPLCRRFTVHHALHQVIEESRDSIVLIFLDDIPDHKLNHCLYIRRGMLNSRCILNWPLQRTRIPAFHQHLKVALGLSNRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N919</accession>
    <name>W5N919_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kelch-like family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007319">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007322">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632695.1">
        <property type="nucleotide sequence ID" value="XM_006632632.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N919"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017128"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017158.1">
        <property type="protein sequence ID" value="ENSLOCP00000017128.1"/>
        <property type="gene ID" value="ENSLOCG00000013889.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693950"/>
    <dbReference type="KEGG" id="loc:102693950"/>
    <dbReference type="CTD" id="56062"/>
    <dbReference type="eggNOG" id="KOG4441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159818"/>
    <dbReference type="HOGENOM" id="CLU_004253_12_0_1"/>
    <dbReference type="InParanoid" id="W5N919"/>
    <dbReference type="OMA" id="AWTVMPP"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013889">
        <property type="expression patterns" value="Expressed in heart and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18510">
        <property type="entry name" value="BACK_KLHL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18336">
        <property type="entry name" value="BTB_POZ_KLHL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000063">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000086">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000027">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF26">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="183"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="719" mass="80537" checksum="91C5BD13895574F8" modified="2014-04-16" version="1">MSVSGKKEFDVKQILRLRWRWFSHSSQSSANSGSYIQQEGFEHRGTPVQNRLKSHSRDRSGLRKTGSPVHSILAPTPGPIPVYARPPNQSWHQQNIIEHLQVNDELPKIGSPENYRKEDGKLGKEDVKATSEDPSEEEARERLSLSNYSRMNSNCSDEFFQAMNHAEQTFRKMENYLQHKQLCDVLLIAGNHKIPAHRLVLSAVSDYFAAMFTNDVREAKQEEIKMEGVDPDALKSLVHYAYTGVLELKEETIESLLAAACLLQLSQVIEVCCNFLMKQLHPSNCLGIRSFADAQGCMDLLNVAHSYTMEHFLEVIQNQEFLLLPSAEIVKLLASDDINVPDEETIFQALMMWVRHDVQSRQQDLGLLLSYIRLPLLPPQLLADLENNKMFADDLECQKLLMEAMKYHLLPERRPMLQSPRTKPRKSTVGALYAVGGMDATKGATTIEKYDLRTNTWIQVGTMNGRRLQFGVSVIDNKLYVVGGRDGLKTSNMVECYNPITKVWTTMPPMSTHRHGLGLAVLEGPMYAVGGHDGWSYLNTVERWDPQARQWNYVASMSTPRSTVGVSALNGKLFAVGGRDGSSCLRSMECFDPHTNKWSMCAPMTKRRGGVGVATYNGFLYAVGGHDAPASNHCSRLSDCVERYDPKTDAWTTVAPLSVPRDAVGVCLLGDRLYAVGGYDGQTYLNTVESYDAQNNEWNEEVPLKIGRAGACIVVVKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9P4</accession>
    <name>W5N9P4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromosome 17 open reading frame 49</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196244.1">
        <property type="nucleotide sequence ID" value="XM_015340758.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196245.1">
        <property type="nucleotide sequence ID" value="XM_015340759.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196246.1">
        <property type="nucleotide sequence ID" value="XM_015340760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9P4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017353"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017384.1">
        <property type="protein sequence ID" value="ENSLOCP00000017353.1"/>
        <property type="gene ID" value="ENSLOCG00000014080.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685177"/>
    <dbReference type="eggNOG" id="KOG4834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014721"/>
    <dbReference type="HOGENOM" id="CLU_104449_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9P4"/>
    <dbReference type="OMA" id="SMKRHAY"/>
    <dbReference type="OrthoDB" id="10021571at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014080">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397:SF2">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="21336" checksum="28C66CF2F05C9B1D" modified="2014-04-16" version="1">MTSASTKVGEIFSAAGAAFTKLGELTMQLHPVADSSPAGAKWTDTEIDMLRAAVRRFGDDLNKISSVIKDRTVSAQIKTTVKRKLYEDSGVPLSSESPKKTPKKTQVSVATTPAVITVPTSQVVVTTALQDPSPAPSAIKKQKTADVTLSALNDSDVNSDLVDIEGLETGSTTKKLNFDQESLNLDSSLIMNSSDLPLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAN0</accession>
    <name>W5NAN0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006728">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006730">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAN0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017689"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017721.1">
        <property type="protein sequence ID" value="ENSLOCP00000017689.1"/>
        <property type="gene ID" value="ENSLOCG00000014366.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162179"/>
    <dbReference type="InParanoid" id="W5NAN0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014366">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000464">
        <property type="entry name" value="Zinc finger and SCAN domain containing 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001076">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002541">
        <property type="entry name" value="Zinc finger protein 732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="37796" checksum="1E07EA0358AE4AE2" modified="2014-04-16" version="1">EEEEEAPGRAPVQGSPSRGVGQGTGKGAGGGDTRSPPGTKPHVCAECGKGFEWLQSLKEHRRVHTGERPYRCQLCGKSFLSSGHLRQHQQTHADSKPHQCAACGKRFSRAKNLEVHQRVHTGERPYLCAVCHKSFPYSSTLKQHLRTHTGERPFQCAACGKRFARAKNLKVHERVHTGARPYRCGVCGESFAKHSSGQVRYQQVDPDHKPYACSECDKAYKRMDHLKRHQQMHSGEKPFHCGTCEKRFSLRRSLEYHQRQCTGESYCKCAVCGDVFQSFSNLHRHQLIHAGEKTYQCSVCGQQCKQSNYFKKHQKVHTEDEPYYCFECEQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB60</accession>
    <name>W5NB60_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SEFIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB60"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017869"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017901.1">
        <property type="protein sequence ID" value="ENSLOCP00000017869.1"/>
        <property type="gene ID" value="ENSLOCG00000014518.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYE0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111286"/>
    <dbReference type="HOGENOM" id="CLU_764945_0_0_1"/>
    <dbReference type="InParanoid" id="W5NB60"/>
    <dbReference type="OMA" id="CSCENNE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014518">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF12">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="9">
        <location>
            <begin position="68"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="318" mass="33426" checksum="C7E5157EF254D0DD" modified="2014-04-16" version="1">QAAVQGMDRALGPWCPFAAYRGRWSLPVLVGLLLISLTVLAACVLQGTLKSWVLKVCKLEDIKGAVCRGQVVLLYPPDDEPALPALVCRLGSALAALGFSVSLDLWSQAELSALGPVPWLHARLGQLQRHGGKVVLILTRGAWERAEAWARGRLVPSGSPYSDVFSASLSCILADCLQGRAGERFLLARFESAPLLPLHAREGSHGTGSLLLPEIFRGLPLYSLPSQSLGFLTEISGPPPHLPAGSRGPSRGRRAAGLRAASRVLAGRLRGGWAGAGVGCSLSGCSQGSAGSGLGLHEEMWETVPLQPHGTPHCTPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC04</accession>
    <name>W5NC04_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:12706"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="W5NC04"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018163"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018195.1">
        <property type="protein sequence ID" value="ENSLOCP00000018163.1"/>
        <property type="gene ID" value="ENSLOCG00000014753.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102696422"/>
    <dbReference type="eggNOG" id="KOG1661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183032"/>
    <dbReference type="InParanoid" id="W5NC04"/>
    <dbReference type="OMA" id="QDSPCPI"/>
    <dbReference type="OrthoDB" id="73890at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014753">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050848">
        <property type="term" value="P:regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000027">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF19">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869138" description="Protein-L-isoaspartate O-methyltransferase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="252" mass="27069" checksum="C8B682A2663BB6A4" modified="2014-04-16" version="1">MRLVSLLATVAFPMGVLLSRTMARLSNGRTHAELITRLKDRGVITSDSVFNVMLATDRGHYTKDFPYMDAPQSIGYKATISAPHMHAHALEVLRDKLTEGATALDVGSGSGYLTACFARMVGCSGRVIGIEHIAELVEESIRNVQADDPDLLTSGRVKLLVADGRQGFPEGAPYDAIHVGAAAAAVPKALLAQLKPGGRLVLPVGPEGGSQVLEQYDRQSDGSFSRKALMGVIYVPLTDKDHQWPRYDDGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE62</accession>
    <name>W5NE62_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Polynucleotide phosphorylase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01027784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE62"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018921"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018953.1">
        <property type="protein sequence ID" value="ENSLOCP00000018921.1"/>
        <property type="gene ID" value="ENSLOCG00000015375.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1067">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014001"/>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_1"/>
    <dbReference type="InParanoid" id="W5NE62"/>
    <dbReference type="OMA" id="RFMFHYN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015375">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000958">
        <property type="term" value="P:mitochondrial mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09033">
        <property type="entry name" value="KH-I_PNPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11363">
        <property type="entry name" value="RNase_PH_PNPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11364">
        <property type="entry name" value="RNase_PH_PNPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000032">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000113">
        <property type="entry name" value="polyribonucleotide nucleotidyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000044">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000008">
        <property type="entry name" value="polyribonucleotide nucleotidyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008805">
        <property type="entry name" value="PRK11824.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252:SF0">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="693"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="796" mass="87079" checksum="8135CC530CBA30E2" modified="2014-04-16" version="1">MAAYRRCGAGISRLCSHSGALLARSPSMRHFLGLGSRVAWAQARRCHQSAWSPGTVRRVAVDAGERKLEISTGKLARFADGCAVVQLGDTSVMVTAVSKTKPTSSQFMPLVVDYRHKAAAAGRIPTNYLRRELGSTDAEILASRLIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNDPDVLAINGASAALALSDIPWNGPIGAVRVGLVDGQFLVNPTRKEMASSTLNLVVAGGPHSQVVMMEASAENVLQQDFCHAVKLGVKHAQQIILAIQQLAREQKVTKRTPQKLFVAVPEMVEQTRQVYFDPGYSTPIKHISDWQVSRDEAIKKVRLDTEELVKEKYPEADPYEVIEAFNTVSKETFRNLVMNEYRRCDGRDLTSLRSISCEVDIFRPLHGSALFQRGQTQVLCTVTFDSLESSIKTDLITSAMSGVKDKNFMLHYEFPPYATNEIGKMGGMNRRELGHGALAEKALKPVIPKDFPFTIRVTSEVLESNGSSSMASACGGSMALMDAGVPVSSAVAGVAIGLISKPNPEKPSEIQDYRLLTDILGIEDYHGDMDFKMAGTSKGITALQADIKIPGLPLKIVMEAIQQATVAKREILGIMNKAIPKPRPSRKENGPVVETVRVPVSRRVRFVGPGGYNLRRLQAETGVTISQVDEETFSVFAPTPAAMHEAQELITEICKDDQEQQLEFGAIYTATITEIRDVGVMVKLYPNMTPVLLHNSQLDHKRIKHPSALGLEVGQQIQVKYFGRDPTDGRMRLSRKVLQSPMATVVKSLSDRQSIAMGTGSAQDSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFA9</accession>
    <name>W5NFA9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PET117 cytochrome c oxidase chaperone</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET117 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFA9"/>
    <dbReference type="FunCoup" id="W5NFA9">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019318"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019350.1">
        <property type="protein sequence ID" value="ENSLOCP00000019318.1"/>
        <property type="gene ID" value="ENSLOCG00000015693.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S7B1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000059926"/>
    <dbReference type="HOGENOM" id="CLU_161486_2_0_1"/>
    <dbReference type="InParanoid" id="W5NFA9"/>
    <dbReference type="OMA" id="WDRERLH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015693">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031568">
        <property type="entry name" value="Pet117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163:SF1">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15786">
        <property type="entry name" value="PET117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867654" evidence="6">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="76" mass="8653" checksum="E67479EEBD35A85F" modified="2014-04-16" version="1">MSTTSKVVLGISLALTLGTVAGVHIKQSLDRERLREGVVRDLERQARKRENLRQLEEQISLTRELEKERERRAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHU2</accession>
    <name>W5NHU2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Centrosomal protein 290</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008652">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008653">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NHU2">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020201"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020234.1">
        <property type="protein sequence ID" value="ENSLOCP00000020201.1"/>
        <property type="gene ID" value="ENSLOCG00000016353.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPTZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111039"/>
    <dbReference type="InParanoid" id="W5NHU2"/>
    <dbReference type="OMA" id="RIEMQQR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016353">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021549">
        <property type="term" value="P:cerebellum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097711">
        <property type="term" value="P:ciliary basal body-plasma membrane docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905349">
        <property type="term" value="P:ciliary transition zone assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042462">
        <property type="term" value="P:eye photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030916">
        <property type="term" value="P:otic vesicle formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032475">
        <property type="term" value="P:otolith formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045299">
        <property type="term" value="P:otolith mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032321">
        <property type="entry name" value="Cep209_CC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026201">
        <property type="entry name" value="Cep290"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879:SF20">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16574">
        <property type="entry name" value="CEP209_CC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomal protein of 290kDa coiled-coil region" evidence="10">
        <location>
            <begin position="1289"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1821"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1884"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2038"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="66"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="698"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="784"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="900"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="962"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1087"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1290"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1372"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1467"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1535"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1623"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2282"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2351"/>
            <end position="2422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1837"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2046"/>
            <end position="2059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2475" mass="289309" checksum="042E8381E801E1E4" modified="2014-04-16" version="1">MPLKIDWGELMDVDPDALTDQESLAQKLLDFISQVEKRDLKGDSPEKIIHLFRITQAIMKMKAQEVDLALEEVEKAGEEQAKIENQLKAKVYKLEHELEMAQRTAGGRDTRFLRDEIRQLENQLDRKEKELAQVEKEINKEKKANEELTLRAEDAENENRKLKREIKHLKKKNEQLRQDIIYYRRELDQKETVQYTEKEKEAQANLAIVNRELHECLDELQRVEAERDELKAQNDNAQKALLDATKEMMVMEEDYSRMKITVKQSDILMDQMKKESEHLKLQVQELTAQIQAKAEEDDPVMAAVNLKVEEWKRILSAKDDEIMEYQQMLRELKEKLRVTQLDSDKNNVMALQQAVQERDSQIKMLTEQVEQYTSEMETNAIVIEELKKPLQKTRGICVIQQKKTEELQAKLQVAEQRVRDAERVAELAESDAKEKDKELSDALSRMTAYEAGVYGLENAVAEIKEYKKQIKTREQEMEYLTREINKQEMKINDLLDENEDFRERLGLNPKEVVDLSEFRRTKTLKQQQYRAENQILHKEIERLEEERLNLKRHIRKLVKDKGRVTYNYVSTLLGLSGDYLQLEDDFSRTSISFEKAKFTSSSINQEEMRDKNEHLLKELNDKDKELQLRMKDSAEFKAKFDELLKEKKYLEEGMKEILQVIKDAREKSQGDVVLHISSLERLINIIEVRNSPGGFDACVHLKTQVDQLTGKNEELRKEMKEARKEAANASSQLIKANEKVEHLECEIEALKQSRGSSVVFKTLNLPEEMALSSMEVISALNEYAVSLLQELKNKEATEKVLEEALEEYKRKFAVVRHQQGLLYKEYQSERESWLNEKEKFTELKNRLEEQKELDAVKIKEYNHWLEVLEMDPSDTKKQISEAARKITVLRVNEKSLTRRYTTLLEMEQHLRKENNRLKNEILEMNSAVADRIGYLQRYKDMAAFKIAALQKALDNSVSSSELERANKQYNELTIKYRDTLQRDNQLVWKTTNLEHLEGENLSLLEKIDGLNRELEITKEKLHTLEQAWEQVTKLGGEGTMDKATKAITNSEILSISKKITMLEMKELNERQRAEHAHKMYEHLRTSFKRVEDRNFELETKFAELTKQNLEAQKIEQELRDELSNSVSKAVSDADRRRIMELEKIEAELKMEVSKLRDISEVAKMQVSALEARQQSREKEVEALRRQVLDYQAQSDEKALVAKLHQHIVALQMSEATAVSKLEAALVKGQKLEAYSLRMEQKLDDKEQALYYARLEGRNRAKHLRQTIQSLRRHFSGALPLAQQEKFSKTMIQLQNDKLKILQDVQRTEQEKRKTEEKALELEIKMKGLEELISTLKDVKGAQKVTEWHKKMEELRLQDLKRSREFGFQKEEIKYLKNVIAGQERTISSLEEEVIQQKNLHEEQQLSWDQREVELERQLDLYERQQNEVLSTARKFEEATGNIPDPSLPLPQQLDFALRKIKEHLRTILETQSTCKILEEKLKEKEEALWKAEQNVLSRDKVINELRLRLPATAEREKLIAELGKRGEDPEYQQALKVAHQTIANLQARLNQKEDIVKKYQNLLARARQEQEDMTKKHEEQIRVLHQKLDLHSDTSLNKFKQTALELMKKPNIAVPTTKHLVRLAEMEQTVAEQDNSLSSLTEKLKRVTADLEKQKQITSMKMKEHENEKAKLEGKYATQSKKLREEADELREQLSQTEKEVQYLKTELEAQKEANVKSPTATMKNLVERLKSQLMLKEKQQKALSKALLELRSEMTAHAEQQIIASAAQKEQNLNVQQIVDKHTKDLKSRIQDLSEELQTAKDSLKMIKNRENSLKDEVESLNRELQRSQKSQSKLQTERDELDKENEELKKRIKRLTSSLQNKSDGEGKCATLDELQKKVKRLESELESKNTEPPVERKPMKEDKTTKEEIIRWEESKKWQAKLESVRNKLKEKEKEVDTLTKQLSTLKDLYARTDQEKAALQKKLKSRGVTVDQVVGARTLESEKMIEELKRRNSDLEHQIVLIKQQQALPRDIAMEDLNLKNRYLEEKIHSLEKQLTKEPLSRPSTSGRGSDTPSQAEHELQRQNLKLSSENLELRFQLEQARKDLPRLNDQVADLKEMCDIVKKEKTELERKLGNVRGSGRSGKTVPELEKTIGLMKKVVERVQRENEALKKAPGIVSREQLTALENENEKLKSDYEKLKLQVGGQLSMRYESKTKGMEKIISENERLRKDLKKEAEAAEKLRIAKNNLELTNEKLRVQLEETNQRLSIAESRGPQLEGADSKSWKSIVVTRMYESKMKELESDIARKNSSITDVKQLLKEAVEREQKTRLVINDLKEQIEILKHFPEDAKTESGLAKELQLFRLTNSRLEKEKAELVHQLAIYRQQRGAEADSTTEPEKAMKHDKLMEELVELQTHLKAADLDKRHMQEEVKKMKKELENFDPTFFEEIEDLKFNYNVEVKRNILLEEQLKKLSQQFGVEVDIPTNVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI95</accession>
    <name>W5NI95_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BICRA like chromatin remodeling complex associated protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001700">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218620.1">
        <property type="nucleotide sequence ID" value="XM_015363134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI95"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020354"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020388.1">
        <property type="protein sequence ID" value="ENSLOCP00000020354.1"/>
        <property type="gene ID" value="ENSLOCG00000016477.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692438"/>
    <dbReference type="KEGG" id="loc:102692438"/>
    <dbReference type="CTD" id="23506"/>
    <dbReference type="eggNOG" id="ENOG502QPQ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159766"/>
    <dbReference type="HOGENOM" id="CLU_011632_0_0_1"/>
    <dbReference type="InParanoid" id="W5NI95"/>
    <dbReference type="OMA" id="DQFHPVP"/>
    <dbReference type="OrthoDB" id="2556847at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016477">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015671">
        <property type="entry name" value="GSCR1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF2">
        <property type="entry name" value="BRD4-INTERACTING CHROMATIN-REMODELING COMPLEX-ASSOCIATED PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15249">
        <property type="entry name" value="GLTSCR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GLTSCR protein conserved" evidence="2">
        <location>
            <begin position="711"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="835"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="974"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="891"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="975"/>
            <end position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1043" mass="110947" checksum="4564B5237C92B693" modified="2014-04-16" version="1">MDDDDDRRLLDIIGDVQALNDYLHGSNSKSIDEDDLNSATFGSANSLFASASNADPSSTLKDVSNHLGNTGGDGLQLSSSLQFIEEELGGASPSMGELGEEQPFDILQKSLQEADITEQTLAQEALLESSSPSLGVAGQPFSQQVVPNSSFGAMGLSHLPVLQPPAGSHFPGMQPHGFIHQVHPQHLHNGSPSGHIQLVGSFNTQPPMMTINNLDRPQILLKPGQPSSPGMGGGLLVQRQPSHNAGGGPMFSNSAPGQVSMPFKGCGLQAPIPVQNIIIQRGPSSQPLSTMNQNLKGPISIQPKPIQVGHQTVYNISSLGIQPPPSQHQAQQSSPYASANSPQAGQQQSQQSQQMTLNIVNQPGLRKPTSGHQVINHSGSIVIQSPMGAQHQQQSSLHQNQFLLPTSLSISPSCTATHSIQALNGQVLQTQQAQLIHNPADQAFGNRTLANPSYSGAILANQSTSVQLVAGQNFASSGGQLIVNQGVVSSQLQQPPQTVVHLSASHGNVPKARPGFPAGGQAGSSMVQGMQIPNRFAVINSAGSVHPGYGAQSQQAVQGASAQQNLQQQQAPVRQQVPANMGHRFFPPASEEAVNSSQTLGNQGAQHQQVQQQQHQQCETHQFGQNQTQKEAGLFPKLQDGLRQPQLANLLGNKVNKGLSAASAPLDNVHLANKAEVQHLHQPNTQISAHKRPASQQLTKGNLILQQLRKDQASVQAVDKSPFSSVDDAIKRLLPYHVFQGSLPTDEDFKRVDNEFEAVATQVLKRTQAMLNKYRRLLLVEGKRMSPSSEMVMIDRTFNQEERANLTQDKRLALMDPDGFLEDFCCLAKPTEQAAQEALASQTPSPPNRTEKEGASSPRLTGGPQDTETKTGAPGSEQKQLCGDGVDTRKASLTPSTLLQCQSGCSPAQDRREDTKCKGTLDSAVRKASQPSPSSQSCKDSMGDPPQQEVSLSEHLETAIKSILDLKKTQKCSLNSNNNNNSSSSIVSAPPAPVPHSTTVPPSAPPTAPHPGRGSDKPLEHSQGPLADTDSVLEAAVNSILEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJP0</accession>
    <name>W5NJP0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJP0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020849"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020885.1">
        <property type="protein sequence ID" value="ENSLOCP00000020849.1"/>
        <property type="gene ID" value="ENSLOCG00000016866.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2W8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155908"/>
    <dbReference type="HOGENOM" id="CLU_2037346_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJP0"/>
    <dbReference type="OMA" id="CRTGPRN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016866">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="127" mass="14296" checksum="45D92CAD902E5DD1" modified="2014-04-16" version="1">MESLLDNPMKAVLYLKELTTIVQNQQSLIQTQRQRIDELERKVEELIGENRQLKDHHQAQTQHPPPPPPPPHQQQQLQLVPASPPSPKPGQSSPDPAEERRSPCCKSLVPQTPTTLCRAVGIARKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJY1</accession>
    <name>W5NJY1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GABA(C) receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJY1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020976.1">
        <property type="protein sequence ID" value="ENSLOCP00000020940.1"/>
        <property type="gene ID" value="ENSLOCG00000016939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156864"/>
    <dbReference type="OMA" id="CGIPNTK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016939">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19005">
        <property type="entry name" value="LGIC_ECD_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000007">
        <property type="entry name" value="Gamma-aminobutyric acid type A receptor rho2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008057">
        <property type="entry name" value="GABAAa_rho_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01670">
        <property type="entry name" value="GABAARRHO"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022269023" description="GABA(C) receptor" evidence="18">
        <location>
            <begin position="22"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="61"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="272"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="54054" checksum="B09E0C2C94CCA352" modified="2014-04-16" version="1">MMPYFEKLLFLLFCLILIGECRKHRTRRRRWTGAVEPHKHGFPLSKKTQDVTKTRKVKTDHLLRVDDHDFTMRPAFAGPAIPVGVDVQVESLDSISEVDMDFTMTLYLRHYWKDERLSFPSTTNKSMTFDGRLVKKIWVPDVFFVHSKRSFIHDTTTDNIMLRVFPDGHVLYSLRVTVTAACNMDFSRFPLDSQTCSLELESYAYTDEDLMLYWKNGDESLKTDEKISLSQFLIQKFHTTSRLAFYSSTGWYNRLYINFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVSLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVLEYAAVNYLTTVQERKERKIRDRLPCTCGLAHPRQLIMGTSYSDVDVNTTGTYGVPENGEKQERILVHLALGDDRTTSRRKNSRSYVSMWIDTHAIDKYSRIIFPGAYILFNIIYWSIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMF2</accession>
    <name>W5NMF2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AHAT01003708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632292.1">
        <property type="nucleotide sequence ID" value="XM_006632229.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015205936.1">
        <property type="nucleotide sequence ID" value="XM_015350450.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015205937.1">
        <property type="nucleotide sequence ID" value="XM_015350451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMF2"/>
    <dbReference type="FunCoup" id="W5NMF2">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021811"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021848.1">
        <property type="protein sequence ID" value="ENSLOCP00000021811.1"/>
        <property type="gene ID" value="ENSLOCG00000017706.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696707"/>
    <dbReference type="KEGG" id="loc:102696707"/>
    <dbReference type="CTD" id="4247"/>
    <dbReference type="eggNOG" id="KOG2791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007341"/>
    <dbReference type="HOGENOM" id="CLU_032753_2_1_1"/>
    <dbReference type="InParanoid" id="W5NMF2"/>
    <dbReference type="OMA" id="ISEKFMA"/>
    <dbReference type="OrthoDB" id="6019616at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017706">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="360"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
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        <location>
            <position position="73"/>
        </location>
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        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
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        <location>
            <begin position="325"/>
            <end position="426"/>
        </location>
    </feature>
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        <location>
            <begin position="364"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR607754-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="433" mass="50719" checksum="0E9C0BF9A272C3F9" modified="2014-04-16" version="1">MRFRIYKRKVFILALVVLACGFAFWSSGRQKKSELFSKEVDNVRSNRVHSTSGNRRVFNETQPEKVQKPEVDNMTLVYRGIVYQLNFDQTIRNVEKFKMRRKDDLVVVVQVHNRPEYLKLLIDSLRKAKGIENILLVFSHDYWSPEINQVVATVDFCQVLQIFFPFSIQLYPQEFPGNDPKDCPRDIPKKDALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKALKDHNGLVLLIEEDHFLSPDFYHLLQMMYTLKREQCPDCDILSLGGYAHSGFSSKASKVEVKAWKSTEHNMGMAMTRETYQQLIQCTDTFCTYDDYNWDWSLQHLTVTCLPRFWKVMVSEAPRIFHAGDCGMHHKKACMPSTQKAKIESILQSNSNLLFPKTLLINTKQPLVGVSPHVKNGGWGDIRDHELCKSYRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN23</accession>
    <name>W5NN23_LEPOC</name>
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        <submittedName>
            <fullName evidence="7">Trophoblast glycoprotein-like</fullName>
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    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01039765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000017929.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154868"/>
    <dbReference type="HOGENOM" id="CLU_064866_0_0_1"/>
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    <dbReference type="OMA" id="QCYCFGS"/>
    <dbReference type="Proteomes" id="UP000018468">
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        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR052286">
        <property type="entry name" value="Wnt_signaling_inhibitor"/>
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        <property type="entry name" value="LP06937P"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
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            <begin position="23"/>
            <end position="339"/>
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            <begin position="279"/>
            <end position="299"/>
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            <begin position="219"/>
            <end position="270"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="339" mass="38332" checksum="B337A1914F1207B2" modified="2014-04-16" version="1">ISKQPRFFLTLIMSMLLLLANADNLCPFSCACFENTETVTCLNVNMEDVPSDFPLWVQTLTVSGNNITTLIGNAFTTNGTSLELLSTLSLSDNNIQNIEASAFERLPNLKALDLSKNHLTYISSDAFRGLRELRNLSLNSCLTKSAVIQLSSALASDSLGKLKRLELSANHIKSIPPLIFDSFNLEVLILTNNSIQSVEREVVNKWLENKKMHVYLEFNPFVCDCTLESLYLWVKNSSQCPDASRLRCSEPESVKGTLVMRLKKEEVDCRNPDLETVSYVFLGIVLALIGVIFLMVLYLNREGIKRWLNNIREACRDQMEVYHYRYEQDSDPRLANVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN94</accession>
    <name>W5NN94_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">LRAT domain containing 1</fullName>
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    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206681.1">
        <property type="nucleotide sequence ID" value="XM_015351195.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5NN94">
        <property type="interactions" value="684"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022103"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022144.1">
        <property type="protein sequence ID" value="ENSLOCP00000022103.1"/>
        <property type="gene ID" value="ENSLOCG00000018002.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684382"/>
    <dbReference type="KEGG" id="loc:102684382"/>
    <dbReference type="CTD" id="151354"/>
    <dbReference type="eggNOG" id="ENOG502QPSG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161165"/>
    <dbReference type="HOGENOM" id="CLU_082142_0_0_1"/>
    <dbReference type="InParanoid" id="W5NN94"/>
    <dbReference type="OMA" id="IYEHDDQ"/>
    <dbReference type="OrthoDB" id="6157531at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018002">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
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        <property type="entry name" value="LRATD1_LRATD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46341">
        <property type="entry name" value="PROTEIN FAM84B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46341:SF1">
        <property type="entry name" value="PROTEIN LRATD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRAT" evidence="2">
        <location>
            <begin position="110"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="30314" checksum="3BFB99B7DC92EBF4" modified="2014-04-16" version="1">MGNQLDRITHLNYSELPTGDPSGIEKDELRVGVAYFFSDEEEDLDDRSQPENYKDQSPSKDGHLVLNEIEYSAFCCQECIFSKLRENEDLNIYSIKTLLTMCKPGDLLELVHVANDQPPHWTIYEGDDQVIHLHKGEIRKDSLFEISNGRLGRIVNNRYRYRPLPADLVMQNACGHLGLSSGEISWTNSESFAAWCRFGKREFKAGGEVHTAEQQYFLKIHLSKNNVHTLMFHSLEDLIRERRKVDATGKLRLLKELSIVSGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNY1</accession>
    <name>W5NNY1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 10 open reading frame 105</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004005">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5NNY1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022381.1">
        <property type="protein sequence ID" value="ENSLOCP00000022340.1"/>
        <property type="gene ID" value="ENSLOCG00000018239.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00490000043902"/>
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    <dbReference type="OMA" id="IASCIVF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
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    <dbReference type="Bgee" id="ENSLOCG00000018239">
        <property type="expression patterns" value="Expressed in heart and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039954">
        <property type="entry name" value="DUF5527"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38325:SF1">
        <property type="entry name" value="GENE, 17455-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38325">
        <property type="entry name" value="MCG55969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17665">
        <property type="entry name" value="DUF5527"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="134" mass="14606" checksum="E7C8792AE561200E" modified="2014-04-16" version="1">VCCAGKMSELVNSSAVPLNDSWTLLSRSPLPTVSPVPVPDPLPIILAVLCLFVLFASCAAFLALCRPSALERARNGHRESMPHHSGDTSEPQLRLWKRLGSLRQSLSSFRRTQRPPAPCRSAHNPLPCLDATEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP44</accession>
    <name>W5NP44_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G protein-coupled receptor 151</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPR151</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01027394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="W5NP44">
        <property type="interactions" value="967"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022403"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022444.1">
        <property type="protein sequence ID" value="ENSLOCP00000022403.1"/>
        <property type="gene ID" value="ENSLOCG00000018302.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234518"/>
    <dbReference type="InParanoid" id="W5NP44"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018302">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15002">
        <property type="entry name" value="7tmA_GPR151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="40"/>
            <end position="66"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="98"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="139"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="172"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="228"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="266"/>
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    </feature>
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        <location>
            <begin position="55"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="299" mass="33147" checksum="925C4A7E9F2B3172" modified="2014-04-16" version="1">MDKLLTSSFNFSTANSSAHKPTFPEGGGYQHLDSPELKALIPAVLGVICALGFAGNLTAIGVLVTNARKGKLSLINSLILNLTLADGLVLAFGAPFKAAAYAKASWTLGWFVCKTCNWFLHSCMAAKSFTVAVMAKACFRYVSNPTKQVTIRYKTILSILLLCWLLACLVPLPHWLFATLRREGVGLLCVQVIPASASDFMSVYVYLYPLVAFCLPLCFALLYFWRAYRRCQRRGTKTQNLRTQIRSRKLTVMLLSLALTSGTMWLPEWISWVWMRHAAPGGXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP72</accession>
    <name>W5NP72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycogen synthase kinase binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSK-3-binding protein family.</text>
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    <dbReference type="EMBL" id="AHAT01017932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015357328.2"/>
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        <property type="component" value="Linkage group LG11"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010422"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="173" mass="19218" checksum="341BF4FBED257CFD" modified="2014-04-16" version="1">MPCRKENYILLEQSVTVDSKEVDALVTKIGEALQLHSSAGANSQKTLSSCLHHSHSSSGDKHTCTSTKHSYSASHTRTSSNTKHGCCIRLRNRGLRSRASPYSIPGDYSDQDWDSLRAWNRKKIDVSSQEDDPHQLLQELILSGNLIKEAVRRLQLSTADRADLCKPADSLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP83</accession>
    <name>W5NP83_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delayed-rectifier potassium channel regulatory subunit KCNS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Delayed-rectifier K(+) channel alpha subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Delayed-rectifier potassium channel subunit Kv9.2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily S member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Potassium channel regulatory subunit that modulate the delayed rectifier voltage-gated potassium channel activity of KCNB1 and KCNB2 by altering their kinetics, expression levels, and shifting the half-inactivation potential to more polarized values. While it does not form functional channels on its own, it can form functional heterotetrameric channels with KCNB1 and KCNB2. Each regulatory subunit has unique regulatory properties that can lead to extensive inhibition, significant changes in kinetics, and/or substantial shifts in the voltage dependencies of the inactivation process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterotetramer with KCNB1 and KCNB2. Does not form homomultimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the potassium channel family. S (TC 1.A.1.2) subfamily. Kv9.2/KCNS2 sub-subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015357928.1"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000022442"/>
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        <property type="protein sequence ID" value="ENSLOCP00000022442.1"/>
        <property type="gene ID" value="ENSLOCG00000018341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687778"/>
    <dbReference type="KEGG" id="loc:102687778"/>
    <dbReference type="eggNOG" id="KOG3713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160344"/>
    <dbReference type="HOGENOM" id="CLU_011722_4_1_1"/>
    <dbReference type="InParanoid" id="W5NP83"/>
    <dbReference type="OMA" id="CCSGAYH"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018341">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily S member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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        <property type="entry name" value="BTB/POZ_dom"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
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        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
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    </dbReference>
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        <property type="entry name" value="T1-type_BTB"/>
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        <property type="entry name" value="VG_K_chnl"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="17"/>
            <end position="126"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070342"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077017"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00080555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="54136" checksum="AD9DFF7C4D593128" modified="2014-04-16" version="1">MTGQSLEDLSGADFEDSVICINVGGFKKRLTSNTLSRFPDTRLARLLRCQSKESILELCDDYDDAEKEFYFDRNPALFPYVLNFYNTGRLHVMAELCIFSFSQEIEYWGINEFFIDSCCSNVYHCRKMEPVQEDWEDKSEEGSTTSSFDEILEFYNDASKFDKQPLGSARRRIWLMLDNPGYSVASRIFSVLSILVVLGSIATMCMNSMADFMLADGEGHLLEDPRFEMVEHFGIGWFTLELVARFSVAPDLLRFFEHPLNLIDLMSILPFYLTLVINLVVESSPALANLGRVAQVLRLMRIFRILKLARHSTGLRSLGATLKNSYKEVGLLLLYLSVGVSFFSVMAYTIEREENEGLATIPACWWWATVSMTTVGYGDVVPRTIAGKLTASACILAGILVVVLPITLIFNKFSHFYKRQKQLEIAMRSCDFDEGMKEVPSVNLRNYYAHKVKSLMASLSNMSRSSPSEQSLNESMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVN8</accession>
    <name>W5LVN8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 1 open reading frame 230</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="W5LVN8"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15683">
        <property type="entry name" value="TDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="192" mass="20914" checksum="A50C22A1E2FD9E09" modified="2014-04-16" version="1">MNPALWKTYKSKVLKTLNPDFEEDAVEQTKSNEVETDLAATPEDEGASAVSQLARKMQGAGAKGWKSMSALFSREDEHKLLASESQPAADHPLAPKQEEPRLNKRATGFWDSFATKWQQQSAAMKEAESELAGEVVPEDAGSTPEEGGIEEGESNNSTKYTTLGGAEDTGFKWNFVTSKLAELKSKSQAKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW11</accession>
    <name>W5LW11_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LW11"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000318"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000318.1">
        <property type="protein sequence ID" value="ENSLOCP00000000318.1"/>
        <property type="gene ID" value="ENSLOCG00000000291.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163301"/>
    <dbReference type="HOGENOM" id="CLU_1834506_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW11"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000291">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF327">
        <property type="entry name" value="CYTOCHROME P450 2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="140" mass="15361" checksum="89B702D31C122EE4" modified="2014-04-16" version="1">MEEKILEEASHLISHLEKCSGQSMDPKTLFHNAVSNIICAILFGARYNYEDETFQSLIRMVCEITKILNGPWGMVCLLLNIPNISCPHCVKCGILMNGNKGANKEGIESSDAFPSLPCSESQVEGQDCHIVSEQQSGSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW97</accession>
    <name>W5LW97_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionyl/Leucyl tRNA synthetase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LW97"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000404"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000404.1">
        <property type="protein sequence ID" value="ENSLOCP00000000404.1"/>
        <property type="gene ID" value="ENSLOCG00000000371.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075017"/>
    <dbReference type="HOGENOM" id="CLU_1045702_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW97"/>
    <dbReference type="OMA" id="MCATEYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000371">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000174">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000640">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="31102" checksum="4C5D6E4E98B8F8CF" modified="2014-04-16" version="1">MCKQTPVVKSSRHLFLNLPKLESRLESWLDQSISTGDWTANARFITRSWIRDGLKPRCITRDLKWGTPVPHPDFSDKVFYVWFDAPIGYLSITANYTDQWEQWWKNPDQVQLYNFMAKDNVPFHSVVFPCSLLGAEDNYTLVNHLVATEYLNYEDTKFSKSRGVGVFGDMAKETGIPTDIWRFYLLIMRPESQDSTFSWADMALKNNSELLNNLGNFINRAAMFVSKFFESCVPAMELQEEDKRLLAVTSWELKQYIQLLERVRYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWC6</accession>
    <name>W5LWC6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWC6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000433"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000433.1">
        <property type="protein sequence ID" value="ENSLOCP00000000433.1"/>
        <property type="gene ID" value="ENSLOCG00000000398.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ5W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183145"/>
    <dbReference type="HOGENOM" id="CLU_002555_11_3_1"/>
    <dbReference type="InParanoid" id="W5LWC6"/>
    <dbReference type="OMA" id="GWNSSCS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000398">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="95"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="201"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="309"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="425"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="460" mass="48786" checksum="3C575ABE63D4A0A5" modified="2014-04-16" version="1">VEVYHHGQWGTVCDDDWDQSDAQVVCREVGCGEAGASVALGSARFGAGRGPILLDNVHCRGNEPTLLSCTSKGLGNHNCGHSEDAGVDCTGIRRVRLVNGTNSCSGRVEVYHHGQWGTVCDDDWDQSDAQVVCREVGCGEAGASVALGSARFGAGRGPILLDNVHCRGNEPTLLSCTSKGLGNHNCGHSEDAGVDCTGRRVRLVNGHNNCSGRVEVYHRGQWGTVCDDDWDQSDAQVVCREVGCGEAGTPVALGSARFGAGNGPILLDDVRCRGNESTLLSCTSKGLGKNNCAHSEDAGVDCTDSRGRVRLVDGPNNCSGRVEVYHRGQWGTVCDDDWDQSDAQVVCREVGCGEAGAPVALRSTRFGAGSGPILLDDVRCRGNEPTLLNCTSRGLGNHDCRHIEAAGVDCSGAVDFWETTGNRRVRLVDGPNNCSGRVEVYHRGQWGTVCDDDWDQSDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWF6</accession>
    <name>W5LWF6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="28">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Postsynaptic cell membrane</location>
            <topology evidence="28">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LWF6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000463"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000463.1">
        <property type="protein sequence ID" value="ENSLOCP00000000463.1"/>
        <property type="gene ID" value="ENSLOCG00000000420.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158016"/>
    <dbReference type="HOGENOM" id="CLU_007257_2_0_1"/>
    <dbReference type="InParanoid" id="W5LWF6"/>
    <dbReference type="OMA" id="VHMLYPL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000420">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035235">
        <property type="term" value="P:ionotropic glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06379">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13719">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000010">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000025">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000012">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000204">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000025">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000087">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049872">
        <property type="entry name" value="NMDA1-like_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049873">
        <property type="entry name" value="NMDA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 28" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20 28" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 28" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19 28" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18 28" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="16 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="12 28" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="598"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="667"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="849"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="30">
        <location>
            <begin position="471"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="31">
        <location>
            <begin position="477"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="904"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="924"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="971"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="724"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="768"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="26">
        <location>
            <position position="700"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="26">
        <location>
            <position position="730"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="780"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="986" mass="111385" checksum="8C4B2150F6C18D2C" modified="2014-04-16" version="1">MHQKQRVRVLFNNVLKQQDDYCTSCLMHVHPPLLLRGGCEPKMVNIGAVLSQKRYEQVFKDAVTQANQLYGKEKFKMNAISVTHKPNAIQMALSVCEDLISSQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAHVWFDMMREFRWNHIILIVSDDHEGRAAQKRLETLLEEKETKNKNRNYENLDQLSYDNKRGPKAEKVLQFNQDTNLTALLQEAKELEARVIILSASEEDAAAVYKAARHLNMTGSGYVWLVGEREMSGKALSEAPDGLIGLQLINGKNESAHINDAVAVVAQSILELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRKFAHYSILNYQKSRLVQVGIYNGTQVVLNNQRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMHDGTCKEEHTVNGDLIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAKTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDNKEVPGREVLGRAGTPRLPSTSLEKQDDRKSGRAEPDPKKKASFRSISSTLASSIKRRRSSKDTQYHPTDITGQLNLSDPSVSTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXU7</accession>
    <name>W5LXU7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXU7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000954"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000958.1">
        <property type="protein sequence ID" value="ENSLOCP00000000954.1"/>
        <property type="gene ID" value="ENSLOCG00000000855.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWF4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165186"/>
    <dbReference type="HOGENOM" id="CLU_079378_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXU7"/>
    <dbReference type="OMA" id="VTSKMYT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000855">
        <property type="expression patterns" value="Expressed in mesonephros and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003927">
        <property type="entry name" value="Claudin16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01447">
        <property type="entry name" value="CLAUDIN16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="252" mass="28544" checksum="377E48F9E5EA84A9" modified="2014-04-16" version="1">MSPACSKWSPPELRMIVVLQFMAFCLALVSTVFLIVATWTDCWMVNADDDLEVSQKCRGLWWECVTNTQDGIRTCDQYETILAEHPLKIVLTRALMITADILASFALIILVLGLDFIKLFKEEPHIKLRICYFAGYIFGIAGIPGMIGSVWYAVDVYVERATLVLKNVYLGMHYEFGWSCWLGMAGSTGCFLTSIVLTCCLYIFRERIDLVPDNDLEINTAPYYTNTAQEVQKDNEYGRTAAGKKYAMDSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY34</accession>
    <name>W5LY34_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LY34"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001045.1">
        <property type="protein sequence ID" value="ENSLOCP00000001041.1"/>
        <property type="gene ID" value="ENSLOCG00000000931.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RTUU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000270308"/>
    <dbReference type="HOGENOM" id="CLU_1040276_0_0_1"/>
    <dbReference type="InParanoid" id="W5LY34"/>
    <dbReference type="OMA" id="QCASIYS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000931">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="268" mass="28372" checksum="827E75501383EAE6" modified="2014-04-16" version="1">CVTVINSVYISVLQCVTVINSVCISVFQCVTVINSVCISVFQCVTVINSVCVSVLQCVTVINSVCISVLQCVSVINSVCISVFQCVTVINSVCVSVFQCLSVINSVCISVFQCVSVINSVCISVCVTVINSVCISVCETVINSVCISVFQCVSVIKSLCISVFQCVSVINSVCISVFQWVTVINSVCISVFQCVSVINSVCISVVQCVSVINSVCVSVWHNVCVSLFQCVTVINSVCISVLQCVTVINSVFISVLQCVTGINSVCISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZD3</accession>
    <name>W5LZD3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 30</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030135">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZD3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001490"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001495.1">
        <property type="protein sequence ID" value="ENSLOCP00000001490.1"/>
        <property type="gene ID" value="ENSLOCG00000001300.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRWM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165031"/>
    <dbReference type="HOGENOM" id="CLU_269471_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZD3"/>
    <dbReference type="OMA" id="ENYVDHI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001300">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052825">
        <property type="entry name" value="CCD-Prefoldin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031476">
        <property type="entry name" value="DUF4686"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34479">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34479:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15742">
        <property type="entry name" value="DUF4686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="941"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1196"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1218" mass="140217" checksum="91F834A59871A62B" modified="2014-04-16" version="1">MEEPEERAELGDIAQRLREDGLTPGATADERLRHLWRLFLRSEGSLLSVTQDLQALRTQQAAEMKEVENYVDHIRNLSEERETLTAEYERENEQLRLDLQQLRVQQAEAQLKEVEEMLEQEGLEEITHSSPSEQIAYLLVERATLLEKLEAAERKLDTQSLTGSLREVHLQEELDQIRQTLEEELRQQRESMQRTKETMNKAHGAELSDERLARQRLERDLDEASRRLAMAHEEIRRLSDELDSARQAGGQCEPELQRANEEVELLKEEVAKLKERDVTELQKAKEQNDRLDREILALRARVRHLDAERRSLLDTVLHSPFSLSFLSHTASVQQGTSTVRFECALLDEDVTDCGRRCRQEAEDQACRLRELQRRLLKLQAEHEELVERNEELEALLGEAQNRSKEERERHEGEAEGMRRKIKRLEGELSQLSQDLEGPQLKRMFLSTEDQLKSSSQEKLAVLEGRLTEEQEWRKQLEVDLTAAQGTLKKEREELHRSQGELRSLRMETQALRAGLQESRSLSTSLTQLQGEKDLLEHKVSELERAQARLESDLELQRTASRGAQEELRQSRARASELRAQVGGLKAEVGSLERDRNALRDALAEGRAQLAELQKQLNEGAQDRLRRQEEKQRLSLELQCVREQLHSAREEGRRLRQEAGRDLGSAPPDRPRDEGASQLVSLKVEMGRLQTTLEEERLLASQHQLALQAQISEAQARAKSQDALLQQKGEECRQIRQDAQRTQSLFTSAERELRYEREKNLDLKKHNALLDQEKLRVCAELKQAQARLTQLEQSSASLGGELERVQQRARELELEMARGCQSRQAQHSLEEELCSERARLLAADKKKRQVLELQQQLKSALHQLRLEETRASEADRLHRESKDMAQTLGALRAKLQEEQLQRKRLEQREEELQQQARSLREREASLTRATSELGLRLQQQEARLGVQQAEHSAAEEEHRHTQKANQNLAEQLLSCQQESERLQEELQQVLQQLDLQVRKYDEKQAQHKAKLRKAKQVFVKETSERDGRIQQLENDLALAHSLAEKEQDWIKKVTEENEKLLLEKRHLLKKLSEEEEVGHMNMRTTSTIQHRVNFLEGENRQLQDRTLQLSTQVGALERALRNVQSVCSMEELKKMFPAESLLLNGSVLQPTNPSLAPGMCSPLGILDVIRRVKTGEHPEIGNASLSQPSEIGYLNVTSTAGPTAAREHEDTPSLGSDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M067</accession>
    <name>W5M067_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Collagen alpha-1(XVI) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotrimer. Interacts with FBN1, fibronectin and integrins ITGA1/ITGB1 and ITGA2/ITGB1. Integrin ITGA1/ITGB1 binds to a unique site within COL16A1 located close to its C-terminal end between collagenous domains COL1-COL3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001774"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001779.1">
        <property type="protein sequence ID" value="ENSLOCP00000001774.1"/>
        <property type="gene ID" value="ENSLOCG00000001473.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161782"/>
    <dbReference type="HOGENOM" id="CLU_001074_2_2_1"/>
    <dbReference type="InParanoid" id="W5M067"/>
    <dbReference type="OMA" id="MGNSWQP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001473">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000094">
        <property type="entry name" value="Collagen type XVI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF372">
        <property type="entry name" value="COLLAGEN ALPHA-1(XVI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="15">
        <location>
            <begin position="25"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="278"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="544"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="958"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="996"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1459"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="836"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="888"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1031"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1106"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1146"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1205"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1266"/>
            <end position="1286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1591" mass="155958" checksum="2BC7044ADEED3E8B" modified="2014-04-16" version="1">GARCPVLQVEDWRFQPAELQSNITGFNLVRKFGLLKAPEVKKIRNPRGPIILRLGNLPLIRPTEQVFPRGLPEEFTLVITLLLKKKTARENVYLFQISDPQGYPQLSLDLNGPDRTLSLRAQAREHQGDGFVGCTFSGEGVASLFNFRWHKVALSVRAGAAALYVDCSSIETKPLDDRRVVEPHGHTLLGIRASDGAAAEFDIQQMTVYCHPGLAIQESCCEIPGARCPPDTPKSRRAAESSPRSNLVEVVPQPEGKVQSGTQDAGDKCAPCVQVNQEQRAEKGDRGAPGFHGSKGEKGERGADCVGTEAGFSGSCSEGPKGEKGAKGEVGPAGIGAPGFKGSKGEKGNKGDGGQQGVAGTPGKDGRPGEVCVVGPKGQKGQPGPVGPEGLVGEPGPPGVPGLAGIGVPGPPGIPGGPPGGKGDKGDSGMPGQEGQPGDPGPRGPGGPKGEKGDPCEVCPVLPDGLENTVALQGKPGPKGEPGAPGGGEPGLPGSPGLQGIKGDKGESGSSGLKGEKGESCELCPTLGQLPANLINAQEAKLNMKGEPGEPGATGLSGQKGEKGHTGERGPEGRTGKTGQRGLGGRDGEKGDKGDSGVPGIKGESGFKGEKGDSCDLCPALPAEAGGPQPTIRLAGLPGERGEPGTPGIGKPGEPGRPGSPGLKGEKGNQGLQGDPGTAGSAGVPGLSGEPGRSGLPGPKGEKGDACEMCPSLPEGLKDTVGIPGHKGERGEPGLPGTGIPGRHGQPGLPGATGPPGPKGSQGDPGVAGQGLPGPQGEDGQRGPPGKQGPAGKTGATGPRGVKGEKGSEGPEGPPGPLGPVGIGVMGPPGKDGIPGIPGGDGHPGTSGPKGDKGEPGDCGCQPPTFTNSGRPGAPGTLVTGNFWQPGPQGPPGPPGPPGPPGPQGFIGIPGPQGIPGNDGLPGKPGLPGEVNLLLPSDLSPLKQGLCRDCSQGLPGVPGVIGMKGEKGEQGEQGQPGAGSSDGCEKCFERLQKEEARGDNGNGGPGCPGVPGTPGIPGQRGEKGEQGPRGFPGPPGPMGPPGFPGSPGPSGLPGQQGERGLPGPAGMKGEQGPPGSPGYPGSMGPPGLHGLKGERGNPGTVGQKGESGPPGSPGYQGPAGSPGPKGDHGVSGNPGLKGDQGFQGPPGFPGQPGPPGFPGKAGPAGPQGPPAEKGSEGPRGAQGPMGPPGSPGTPGMQGPPGIEGMDGKDGKPGPRGEPGPVGPIGPRGLPGFKGKTGHTGFPGQKGDMGPQGSPGAPGRPGMEGDPGTPGLQGPAGPPGHLGAAGPMGPPGPAGPPGLGVKGERGQAGERGLPGMVGPPGPPGHPGQSGEPGSDGTPGKDGAPGQPGERGLAGQKGEAGSPGSQGPAGERGRPGLPGGSAKSDSSGIGLMGPVGPRGERGSTGAPGPAGPPGMPGMPGTDGVVNYDEVKNFIRQQVMKIFDERMAYYMSRVQLPVEMVSAPGRPGPPGKDGMPGRQGSPGMPGIPGQIGREGRQGPQGPPGVPGNKGEKGDKGVGEMGDIGPPGAPGVQGPPGYGKMGPPGPVGQQGIPGVPGSPGTPGSPGKQGRCNPSDCMGMMPVEQYQPKSMKGPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0U2</accession>
    <name>W5M0U2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Negative elongation factor complex member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021768">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0U2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001999"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002004.1">
        <property type="protein sequence ID" value="ENSLOCP00000001999.1"/>
        <property type="gene ID" value="ENSLOCG00000001721.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTMJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012665"/>
    <dbReference type="HOGENOM" id="CLU_037919_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0U2"/>
    <dbReference type="OMA" id="ASECHIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001721">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010405">
        <property type="entry name" value="COBRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503:SF3">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR COMPLEX MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06209">
        <property type="entry name" value="COBRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="57105" checksum="054F582B30BD04BF" modified="2014-04-16" version="1">MFAGLPELGISNGEDLKETLTNCTEPLKAIDQFQTENGILLPTLQSALPFLDLHGTPRLEFHQSVFDELRDKLMERVAVIAEGKDADRYVWAGGPRLTRLTRQYPRSERQPRVVQCVRASQLPSSGVVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVSEICSVDPCHKFTWCLDACIREKFVDAKRARELQGFLDGVKKGQEQVLGDLSMILCDPFASNTLVLSTVRNLQELLGQDALPRDSPDLLLLLRMLCLGQGAWDMIDSQVFKEPRLDLEVVTRFLPAMMSVVVDDYTFTVEQRLPTEEKSSLTYPTALPEAYTKFLQENRVACEMGLYYVLHITKQRNKNALQRLLPALAETHNDMALGDIFLHLFTGHLTLLSDDFGTEEFCNAVFDGFLLTSFSSKENVHRHILRLLLHLHHKVLPSRLETLLKALEPPKQAHCPSISWAGNLTKYSLCPSAPPQRQPPLQTGKAPGLRVGSSLWNAKPCPSDPYPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M165</accession>
    <name>W5M165_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CDC45 cell division cycle 45 homolog (S. cerevisiae)</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M165"/>
    <dbReference type="FunCoup" id="W5M165">
        <property type="interactions" value="1513"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002127.1">
        <property type="protein sequence ID" value="ENSLOCP00000002122.1"/>
        <property type="gene ID" value="ENSLOCG00000001829.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009662"/>
    <dbReference type="HOGENOM" id="CLU_005871_4_0_1"/>
    <dbReference type="InParanoid" id="W5M165"/>
    <dbReference type="OMA" id="EDCFMEA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001829">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="570" mass="65878" checksum="9D4564C32B54B1CA" modified="2014-04-16" version="1">MFVSDIRKEFYDVVVNQSVVITQACTGSVICHCCGRCQALFHCDHVQYTLVPVRGWQDLETAFLEHKEQFRYFVLINCGANADLLETLQPEEETVFFICDTHRPVDVINVYNDTQIKLLIRQDDDLGIPAYDDIFRDEDEDEQDSGNESDEGSEPSGKRRRFEEGALERRIERQRERREWEARRREILFDYEQYEYHGTSSAMVMFELAWVMSKDTKDMLWWSVVGLTDQWVHDRIPHMKYVTDVATLQRHVSRHNHRNEDEENSLSIDCMRISFEYDLRLALYQHWSLYESICNSCYTSSSFKLWSIHGQKKLQEFLADMGLPLKQVRQKFNSMDVSIKENLREVIEESSNKYGMKDIRIQTFGVHFGFKNKFLAGDVVHAASALLENVERDEKGTDNFIKALDCLSSRSNIEQLHQGIDLGKKKLMAVQQTVASCICTNLIISQGPFLYCYLMEGTPDVKLFSKPLSLTLLSKYLLKAFVRSTRNKRCKLLPLIMAAPLDTEKGTVIVVGIPPDSESSDKKNFFGRAFEKAAESTGSRTLHDHFDTSVIELKTEDRSKFLDALITLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1A0</accession>
    <name>W5M1A0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RAS, dexamethasone-induced 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1A0"/>
    <dbReference type="FunCoup" id="W5M1A0">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002157"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002162.1">
        <property type="protein sequence ID" value="ENSLOCP00000002157.1"/>
        <property type="gene ID" value="ENSLOCG00000001864.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165566"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_3_1"/>
    <dbReference type="InParanoid" id="W5M1A0"/>
    <dbReference type="OMA" id="SAPQENC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001864">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04143">
        <property type="entry name" value="Rhes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF5">
        <property type="entry name" value="DEXAMETHASONE-INDUCED RAS-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="31082" checksum="4AF1B8B2DCE41193" modified="2014-04-16" version="1">ENLATMIKKMTPSENEFDIPAKNCYRMVILGSTKVGKTSIISRFLNGRFDDQYTPTIEDFHRKFYSIRGDAYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNKDSFEEVQRLKQQIFETKSCLKNKTKENVDVPLVICGNKGDRELYREVQREDIDQLIAGDDKCAYFEISAKKNTNVDKMFRTLFVMAKLPHEMSPDLHRKVSVQYCDMLHRKSIKNKKFKDGDVYGIVAPFARRPSVHSDLMYIKEKAIGGGQGKDKERCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M237</accession>
    <name>W5M237_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 13 member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639532.1">
        <property type="nucleotide sequence ID" value="XM_006639469.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M237"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002445"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002451.1">
        <property type="protein sequence ID" value="ENSLOCP00000002445.1"/>
        <property type="gene ID" value="ENSLOCG00000002096.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691306"/>
    <dbReference type="KEGG" id="loc:102691306"/>
    <dbReference type="CTD" id="64849"/>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="HOGENOM" id="CLU_005170_9_1_1"/>
    <dbReference type="InParanoid" id="W5M237"/>
    <dbReference type="OMA" id="AINTWAM"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002096">
        <property type="expression patterns" value="Expressed in liver and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015746">
        <property type="term" value="P:citrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071422">
        <property type="term" value="P:succinate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF62">
        <property type="entry name" value="NA(+)_DICARBOXYLATE COTRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="600" mass="66526" checksum="B79F7ED54925B55E" modified="2014-04-16" version="1">MEAILVFAKKIWCVRRQLMLILTPIALLPLMFALPAREGKCLYVVLLMAVFWCTETLPLAVTAMLPVCLFPLLGILPSSKVCQQYFVDTNFLFLSGLVMASAIEEWNLHRRIALRVLMLVGVKPAWLIMGMMITSAFLSMWLSNTATTAMMLPIGNAILQSLFGDLEKLKEKCHLKDDAESASQMKLQVLASEKQILTGEERADLSEACDTRSASEIRKESEYQKKVWKGFLISIPYAASIGGTATLTGTSPNLILVGQLKSFFPDCDIINFGSWFVFSCPLMLLFLFLGWLWISFLYGGLNTRLCLQKKDFRAEAETKARAVIEEDYKKLGPVKFAEGAIGFFFFLFAILLFTRDPKFFPGWAQLFNKGYVSDAVTGVSIVSILFFFPSQKPSLNWWFDFRAQNTQNMPLVSWKKAQETVPWNIILLLGGGFAMAKGCEESGLSMWIGSHLQPLESVPPGVAVLLITVVVASFTEFASNTATTIIFLPVLAELAMRLQVNPLYLMIPATIGCSYAFMLPVSTPPNSIAFASGHLQVKDMVKTGVVMNILGVLSLSLAMNTWGLYVFDLHTYPSWARSTNVTTVLPGLLNSTATPLNATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M507</accession>
    <name>W5M507_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037320">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01037321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M507"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003465"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003472.1">
        <property type="protein sequence ID" value="ENSLOCP00000003465.1"/>
        <property type="gene ID" value="ENSLOCG00000002945.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="HOGENOM" id="CLU_010468_5_2_1"/>
    <dbReference type="InParanoid" id="W5M507"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF107">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="8"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="323"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="472"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="511" mass="57358" checksum="24264A18557A8176" modified="2014-04-16" version="1">APDVISHLPELRLVLLGRAGAGKSTAGNTILGREEFQTDRITTLSTKSEGVVAGRQITLVDTPGWDGTLRLDTPKKVKQEIELSASLCFLGPHALLLVIPLDSPTEKAAAKELSEIFYQNAWWHTMLLFTCKESLEGRTIEELIESAGEELHQLLKKCGDRYHVLCTKNRTNPMQIEKLLEKSEKLATENNGVYIKNEILTEQGEKEKHERRRNPKTRSEVDSPEVERPQKQKPREEELVMKEGNRNRQVMNLNLPNPKDKVELGKEPEGKPNSDSTPKALVQPNGFRSHIICFQDKYAYLWEVPQLISTAPNKNGDLEASDLPGLRLVLLGLTGSGKSGVGNTILGQEEFTTSSSAGKKECEIRKGQVFGRQVAVINMPDWFSTEFSLDEIQSQIESYVSLSEPGSYALLLVVPLDQPVGNGRRTGDAIQEVFGEQSVRFTLVLFTHGDVLKEKMSERMLECKDREVTTLQRAGQERETNQRERQDSLQTQDTERTHAVKKSSKLINTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5E0</accession>
    <name>W5M5E0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Toll-like receptor 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5E0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003598"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003605.1">
        <property type="protein sequence ID" value="ENSLOCP00000003598.1"/>
        <property type="gene ID" value="ENSLOCG00000003062.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078350"/>
    <dbReference type="KEGG" id="loc:107078350"/>
    <dbReference type="eggNOG" id="ENOG502S9V5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164586"/>
    <dbReference type="HOGENOM" id="CLU_654771_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5E0"/>
    <dbReference type="OMA" id="CCLNFTG"/>
    <dbReference type="OrthoDB" id="2020019at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003062">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF17">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867331" evidence="15">
        <location>
            <begin position="28"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="16">
        <location>
            <begin position="176"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="424" mass="47275" checksum="C5500F70C8DC842A" modified="2014-04-16" version="1">MSSGGDCWGTAMLWGFLVLWVAPVVEGEEACECPKATILQTFPSAVSSGACCLNYSGSSFGSVKWSLFSQLQALKILDLSSCNITEIQDVAASDGLLLRELFLNHNRITLLPERFLANASNLRAVHLEDNRLDRLPDHFLRASDDLEELYLDFIRPSALALGLLKPSLQKLELGNNSLDCSCALIETLQGYSKPQGVNVTDWSRVLENLTCASPENLNGKRVWMVHSTEVCRSHRLTALFVLLPLALVLVLLFCWCCGRKRKRKEGTFKPAKHANHLSTVDRNGSDWMLDRYHHYKPCEVPLPSEGEKNVLLKNQLMLRPSTALLGSSRDLYEEVEIRLGSVDSLVPPISDITNMASSMDSQEIRQPLEDGKPDVETVSVSEVLKDSADREKLYLTQSTEYYNLVPGIDLEDSDHGEYENVDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6B2</accession>
    <name>W5M6B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Leucine rich repeat containing 38b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with KCNMA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6B2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003920"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003928.1">
        <property type="protein sequence ID" value="ENSLOCP00000003920.1"/>
        <property type="gene ID" value="ENSLOCG00000003320.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163979"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_10_1"/>
    <dbReference type="InParanoid" id="W5M6B2"/>
    <dbReference type="OMA" id="VNCFQRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003320">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099104">
        <property type="term" value="F:potassium channel activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF4">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865771" evidence="15">
        <location>
            <begin position="25"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="16">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="17">
        <location>
            <begin position="186"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00023065"/>
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    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038736"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00082"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003920.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="292" mass="32162" checksum="988B4CA00547817F" modified="2014-04-16" version="1">TMLPCCRWLQPLIVLLSSALLTWGQNCPASCLCPDPHTVDCSGQGLTRLPVSIPLDVRRLLLSDNWIPKIPSDFLVLYSDLVYLDLRNNSLSCIEPGTLSTSSRLVFLDLGCNNLTEIAAGTFGESRSLIKLRLGNNPYLTTVNENAFLGLTSLRELELERNALSGLQVAALSQLPSLRVVRLEGNPWVCNCNFSNLFAWLRDNSDKLPTGVDGMECSLPVDGRRVALSQLSVDSFRECQLTLTLTDYLIIIFSGISVSVAAILASFFLASTVHCFQRWSKANKSDEEDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M969</accession>
    <name>W5M969_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein 4.1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Band 4.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Erythrocyte membrane protein band 4.1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009564">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M969"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004928"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004936.1">
        <property type="protein sequence ID" value="ENSLOCP00000004928.1"/>
        <property type="gene ID" value="ENSLOCG00000004107.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157833"/>
    <dbReference type="HOGENOM" id="CLU_003623_3_0_1"/>
    <dbReference type="InParanoid" id="W5M969"/>
    <dbReference type="OMA" id="EHHTFFX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004107">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Band_4.1_C"/>
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        <property type="entry name" value="EPB4.1_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
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        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
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    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF12">
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    <dbReference type="Pfam" id="PF05902">
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    </dbReference>
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        <property type="entry name" value="Membrane_skeletal_4_1"/>
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    </dbReference>
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        <property type="entry name" value="BAND41"/>
    </dbReference>
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        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        </source>
    </evidence>
    <sequence length="1004" mass="111677" checksum="7F5E50B91FF4F274" modified="2014-04-16" version="1">MNMGIQARFPKIHCPEVFIPHKTEASVVGEAESQQQPQEPEAAQDPGPSESQNPPQEEPPSSPPEEQLKPRSRSSAGRGLSRLFSSFLKRRSQCSEGDGPEAEKGDKEKKEKEREQEAQPAGPEEEVFLKAPIAAPEPELRAEGDPALDLHSLSSAETQPAQEDQENKEDPDTEKDSEEEEGGAEEEEEEEEEEGAEQAEEGKAGGEAEIKAPEEPRAPKAPRKPRSMHCRVTLLDDTLYECELEKHAKGQDLFNKVCDHLNLLEKDYYGLAIWETPTQKTWMDVTKEIRKQVQGTSYDFTFNVKFYPPDPAQLTEDITRYYLCLQLRKDILSGTLPCSFVTLALLGSYTVQSELGEYDPELHGADYVSEVRLAPNQTKELEEKVMELHRTYRSMSPAQADMMFLENAKKLSMYGVDLHQAKDLEGVDITLGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPSEQEQYESTIGFKLPNYRAAKKLWKVCVEHHTFFRLTSTEATTPRRFLALGSKFRYSGRTQAQTRQASSMIDRPAPQFQRTASKRASRSLDGAGGKSPHAELNPVEERKEEDDWFVLMDPNQSQLNPEPSAAGEATPAPPKPAVDQEEKEDDWFVLLDRAASQHTPEPPAGGSVAQPRPTAASAREWQDDWFVLLDTTPSSPAVVPTPDKSTRPVSAPVISPVPRGGEPSLPAPASAAPPADQRELPPAAPQPPKEEQRTAEVKSEAPASEPSRPEPTQEVKVGRERREGPAVTASNGEKAQRPPVADEELVRMRKKRAKKIEGETIYIRHSNLMLEDLDKTQDEIMRHHASISELKRNFMESVPEPRPSEWEKRLSTHSPFRALGVNGQAQPGSDGPPLVKTQTVTISDVSNSVRSDFSTKEVPIVHTETKTITYEAAQLEGSPADRDPGVLLSAQTITSETIGTTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHQETEITPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M987</accession>
    <name>W5M987_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase 47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Deubiquitinating enzyme 47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Ubiquitin thioesterase 47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Ubiquitin-specific-processing protease 47</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01008210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193746.1">
        <property type="nucleotide sequence ID" value="XM_015338260.2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004946"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004954.1">
        <property type="protein sequence ID" value="ENSLOCP00000004946.1"/>
        <property type="gene ID" value="ENSLOCG00000004131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694716"/>
    <dbReference type="CTD" id="55031"/>
    <dbReference type="eggNOG" id="KOG4598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157223"/>
    <dbReference type="HOGENOM" id="CLU_002928_0_0_1"/>
    <dbReference type="InParanoid" id="W5M987"/>
    <dbReference type="OMA" id="CEWIVSK"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004131">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045578">
        <property type="entry name" value="USP47_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF902">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25985">
        <property type="entry name" value="Ubiquitin_USP47_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19718">
        <property type="entry name" value="USP47_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="192"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="831"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="882"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="993"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="831"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="908"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="927"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="939"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="952"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="996"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00026136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1374" mass="156479" checksum="A7CB0EE523D80CC8" modified="2014-04-16" version="1">MEMVPSEENQLVPKEGKFWSCRQSIFDEMKKKFSQIENAADEPRVLCIIQDTTSAKSVNEKLTFNLPASTPLRKLFEDVASKAGYVNGTFDLTWGSGTDMAPLDHSSDSSLAESGFEPGKKNFLHLTDKDGEQPQIASDESGTADSSGLDDSSQDRFIGPLPRDCTVGCSSDYSSPSYSYSSILSKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYNWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVRCLECGYESWRIDTYLDIPLVIRPYGSNQAFGSVEEALQAFIQPETLDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSPFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLENTSSTERSLKSKSSLTFELFSVMVHSGSAAGGHYYACIKSFSDGQWYSFNDQHVSKITQDDIRKTYGGTSGTRGYYSSAFASSTNAYMLIYRLKDPSRNAKYLEVQDFPAHITRLVQKEKDLEEQEKKQREIERNTCKIKLFCMHPVKMLMMENRLEVHKDKTLKEAVEIAYKLMDLDGIVPLNCCRLVKYDEFHEYLERSYEGEEDTPMGLLLGGVKSSYMFDLLLETRRPEQVFQPYKPGEVMVKVHVVDLKTEVIASPVSVRAYLNQTVTEFKQLISQATGLSAETMRIVLERCYNDLRLLYVPNKTLKAEGFFRSNKVFVESSDSTDHQVTFTDSLLWKLLDRHGNTIRLFVSLPEQSPGSHASRTLCQKGGGDSGHLEDNFEMVKGNRKSVEAILEESTEKLKNLSLQQQQGGGNGDSLKSSDASDFEHIESPSQEADSSMSVDNRELENRIQTSDPENNFQSEERSDSDINNDRSTSSVDSDILSSSHSSDTLCNADNAPIPLANGLDSHSITCSRRSKANEGKKETWDTAEEDSGTDSEYDENGKNKGETQYMYFRAEPYSADESSGERQKCLLVHVDKRITLVAFKQNLEPFVGVPSSQFKVFRVYANNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNDPEPCKFLVDAVFAKGMTVRQSKEELLPQLRDLCRLDLSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEPERMKSMSQLAVLTRRWSPAQMKLEPFREAVLESSSVDELKEKLSEISGIPLENVEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNVWPLYICDDGAVVFYRDKSEELMELSDEERNELMKKESSRLLKTGHRVNYSPRKEKALKIYLDGAPNKDSGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9Q3</accession>
    <name>W5M9Q3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Caudal type homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Caudal homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003162">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9Q3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005120.1">
        <property type="protein sequence ID" value="ENSLOCP00000005112.1"/>
        <property type="gene ID" value="ENSLOCG00000004278.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0848">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161261"/>
    <dbReference type="HOGENOM" id="CLU_073177_1_0_1"/>
    <dbReference type="InParanoid" id="W5M9Q3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004278">
        <property type="expression patterns" value="Expressed in intestine and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006820">
        <property type="entry name" value="Caudal_activation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047152">
        <property type="entry name" value="Caudal_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332:SF16">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04731">
        <property type="entry name" value="Caudal_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="143"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="3">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="24190" checksum="73B166B9D3024CC3" modified="2014-04-16" version="1">MHVRHLLDTDANMYPGSVKRAGHYLGAPDFVNPPQYPEYGQYHVSGLNLDGTTQTTTSWHYGPARQDWSAYPQTLQNGLYRLNASPGTAYCPAEYSPGPQSELSIVQALNTNSSECLNANSPYCYQWTNRSTPTANNSSGKTRTRDKYRVVYTDHQRLELEKEFRFSSYITIRRKGQLGTGLAQSSRSQIIYERTRRSVVYKTINKLLNQMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9R3</accession>
    <name>W5M9R3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tumor necrosis factor receptor superfamily, member 1B</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9R3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005130.1">
        <property type="protein sequence ID" value="ENSLOCP00000005122.1"/>
        <property type="gene ID" value="ENSLOCG00000004286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107075583"/>
    <dbReference type="CTD" id="7133"/>
    <dbReference type="eggNOG" id="ENOG502RZ23">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166932"/>
    <dbReference type="HOGENOM" id="CLU_613883_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9R3"/>
    <dbReference type="OMA" id="NGCHENE"/>
    <dbReference type="OrthoDB" id="8633482at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004286">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052459">
        <property type="entry name" value="TNFRSF_decoy_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097:SF116">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867814" evidence="11">
        <location>
            <begin position="20"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="446" mass="47955" checksum="FA1D4007D1400E04" modified="2014-04-16" version="1">MFWKTLGLALAVTMLHVESQMYILPYPPDNRGHVCRNPEREYYRSMVHRCCSQCPPGTRLIEDCTETKDTVCQKCRNGSYTDRWHYHNNCFRCTKACYQGLEEVQPCTVTSPRKCVCKGGYCSFTSSSYSCDTCSMYSSCPPGQGVSEKGTANSDVVCSPCPPGTFSATDSSTEICQPHTDCASHGTSELRAGTNSADAVCHPKMATTPPRAVAAETLGNSVLHRMDAPFSARPPSLPHSVPTTVQADKTVAVIDKTNIIAGAVAGGAVLVTIIIVMFIIGRCIYNRTAALQENADAEAKKVLNCTPGALQRNSSGNLHPPSQERLCLLGETESSQSELSSALGSQSGAETTTSSSQRGSPSCCQDSPQATSPQLKVNFQATISCHLPTNTHCCSPPRAEGVGYSPEPYMFSPNFPLSQEETSISQCQDKDNKDARPAVQEVGKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD17</accession>
    <name>W5MD17_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD17"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006276"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006284.1">
        <property type="protein sequence ID" value="ENSLOCP00000006276.1"/>
        <property type="gene ID" value="ENSLOCG00000005208.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157419"/>
    <dbReference type="HOGENOM" id="CLU_035750_3_0_1"/>
    <dbReference type="InParanoid" id="W5MD17"/>
    <dbReference type="OMA" id="KQHLFRH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005208">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="11">
        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="284" mass="30718" checksum="D212D2CD7E227124" modified="2014-04-16" version="1">KECWRAKMASLSVCQPTCGGFSFEYSKRNALLEAEFTKLGSSIPTARKTGTTICGIVFKDGVVLGADTRATEGMVVADKNCSKIHYISSNIYCCGAGTAADTEMTTQLISSNLELHALSTGRLPRVSTANRMLKQMLFRYQGYIGAALVLGGVDCNGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDRYKQDMEEEEAKQLVRDAIAAGIFNDLGSGSNIDLCVITKGKVDYLRPHDEANKKGVRSGSYKYKRGTTGVLTKAITPLDLEVVEETVQTMDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDV6</accession>
    <name>W5MDV6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1</fullName>
            <ecNumber evidence="3">1.14.11.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lysyl hydroxylase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-[collagen] + 2-oxoglutarate + O2 = (5R)-5-hydroxy-L-lysyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16569"/>
            <dbReference type="Rhea" id="RHEA-COMP:12751"/>
            <dbReference type="Rhea" id="RHEA-COMP:12752"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:133442"/>
            <dbReference type="EC" id="1.14.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Rough endoplasmic reticulum membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.4" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01009797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069040125.1">
        <property type="nucleotide sequence ID" value="XM_069184024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDV6"/>
    <dbReference type="FunCoup" id="W5MDV6">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006565"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006573.1">
        <property type="protein sequence ID" value="ENSLOCP00000006565.1"/>
        <property type="gene ID" value="ENSLOCG00000005408.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686450"/>
    <dbReference type="CTD" id="777635"/>
    <dbReference type="eggNOG" id="KOG1971">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234558"/>
    <dbReference type="InParanoid" id="W5MDV6"/>
    <dbReference type="OMA" id="TDVACNH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005408">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008475">
        <property type="term" value="F:procollagen-lysine 5-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23004">
        <property type="entry name" value="GT_LH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057589">
        <property type="entry name" value="GT_PLOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001006">
        <property type="entry name" value="Procol_lys_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF5">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25342">
        <property type="entry name" value="GT_PLOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25238">
        <property type="entry name" value="OGFOD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01325">
        <property type="entry name" value="LYS_HYDROXYLASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868009" description="Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1" evidence="17">
        <location>
            <begin position="24"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="18">
        <location>
            <begin position="684"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="775" mass="89681" checksum="00A291B67E59D2D9" modified="2014-04-16" version="1">MKTGVLMPLWGILLTILLVKVNCEELQPVPEENLLVLTVATKETDGFQRFMRSAKFFNYTIKVLGRGEKWRGGGYLTPPGGGQKVRLLKSALEKMEKEDQIILFIDSYDVAFASGPKELLKKFHQTRHKVVFSAETLIWPDRHLEDKYPYFREGKRFLGSGGFIGYVPNIKKMVEDWSGKDDDSDQLFYTQIYINPEKRKNINITLDSKCRIFQNLHGALDEVVLKFEDGRVRARNVAYDTLPVVVHGNGPTKLQLNYLGNYIPRVWTFETGCTVCDEDQKLLSGLKESEYPMVLVGIFIQQPTPFVTVFFERLLNLQYPKDRLKLFIYNHEPHHEKQVKSFLENHGTEYRAVKVIGPEEELDSAAPRNLGIDVCRQDTDCEYYFSVDVEVVVKNANTLKILIEQNKPIITPLMTRHGKLWSNFWGALSAEGYYARSEDYVDIVQGRRVGVWNVPYISSIYLIKGSVLKSELLAKDLFSSPHLDPDMAFCHNVRNQGGFMYVTNRQEFGHILSTENYQTSHLHNDLWQIFENPVDWEERYIHVNYSRMMKDKLIETPCPDVYWFPIISDVGCNHLVEEMEHFGQWSHGSNEDTRIQGGYENVPTIDIHMNQVGYEREWHKFLLEYIAPVTEKMYPGYYTKCTSYLNFVVRYKPDEQPLLEPHHDASTFTINIALNSKDTDYQAQFELAFVVRYKPDEQPFLKPHHDASTFTINIALNQVGIDYEGGGCRFLRYNCSIQAPRKGWALMHPGRLTHYHEGLPTTKGTRYIAVSFVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDZ9</accession>
    <name>W5MDZ9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="29">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25 29">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="29">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="29"/>
    <dbReference type="EMBL" id="AHAT01004425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDZ9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006608"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006616.1">
        <property type="protein sequence ID" value="ENSLOCP00000006608.1"/>
        <property type="gene ID" value="ENSLOCG00000005468.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692416"/>
    <dbReference type="KEGG" id="loc:102692416"/>
    <dbReference type="CTD" id="4067"/>
    <dbReference type="eggNOG" id="KOG0197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158011"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_2_1"/>
    <dbReference type="InParanoid" id="W5MDZ9"/>
    <dbReference type="OMA" id="EQTHYVR"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005468">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097028">
        <property type="term" value="P:dendritic cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002431">
        <property type="term" value="P:Fc receptor mediated stimulatory signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050855">
        <property type="term" value="P:regulation of B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090022">
        <property type="term" value="P:regulation of neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002513">
        <property type="term" value="P:tolerance induction to self antigen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12004">
        <property type="entry name" value="SH3_Lyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000553">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000095">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000036">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000010">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035748">
        <property type="entry name" value="Lyn_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 28 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0391">Immunity</keyword>
    <keyword evidence="22" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="24" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0519">Myristate</keyword>
    <keyword evidence="13 28 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="26" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6 27" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11 29" id="KW-0808">Transferase</keyword>
    <keyword evidence="20 29" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="32">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="31">
        <location>
            <begin position="132"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="33">
        <location>
            <begin position="250"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="515" mass="58752" checksum="809FEBEB795A657F" modified="2014-04-16" version="1">MGCKKSKLTGPETGGGLVKKNQSVRTERTIYVRDPTSNKQHAINSSNRDSRLLPGQVFQKLEEQQETGKIVVALYPYEAIHQDDLGFKKGEKLKVLEEHGEWWKAKSLTSRKEGFIPSNYVAQVNTMETEEWFFKEITRKDAERQLLAPANKLGYFLIRESETSKGSYSLSIRDIDAQGADAVKHYKIRTLDNGGYYISPKITFPDITSMIKHYQKQADGLCRKLDKPCVKPKAQKPWDKDAWEISKESIKMVKKLGAGQFGEVWMAYYNNTTKVAVKTLKPGTMSVEAFLEEANLMKTLQHDRLVRLYAVVTKIEPIYIITEFMANGSLLDFLKSEAGNKVQLPKLIDFSAQIAEGMAYIEKKNYIHRDLRAANVLVSESLLCKIADFGLARVIEDDQYTAREGAKFPIKWTAPEAINYGSFTIKSDMWSFGILLYEIITYGKIPYPGMSNSDVMTSIQRGYRMPQPEDCPKELYAIMSSCWKTKPEDRPTFDYMQSVLDDFYTATEGQYQQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME27</accession>
    <name>W5ME27_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Connector enhancer of kinase suppressor of Ras 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNKSR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME27"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006636"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006644.1">
        <property type="protein sequence ID" value="ENSLOCP00000006636.1"/>
        <property type="gene ID" value="ENSLOCG00000005495.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686818"/>
    <dbReference type="KEGG" id="loc:102686818"/>
    <dbReference type="CTD" id="10256"/>
    <dbReference type="eggNOG" id="KOG1738">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159599"/>
    <dbReference type="InParanoid" id="W5ME27"/>
    <dbReference type="OMA" id="DHTEMSE"/>
    <dbReference type="OrthoDB" id="2157866at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005495">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd06748">
        <property type="entry name" value="PDZ_CNK1_2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01260">
        <property type="entry name" value="PH_CNK_mammalian-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09511">
        <property type="entry name" value="SAM_CNK1_2_3-suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000060">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049628">
        <property type="entry name" value="CNK1-3_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051566">
        <property type="entry name" value="CNKSR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017874">
        <property type="entry name" value="CRIC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844">
        <property type="entry name" value="CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844:SF10">
        <property type="entry name" value="CONNECTOR ENHANCER OF KINASE SUPPRESSOR OF RAS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10534">
        <property type="entry name" value="CRIC_ras_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51290">
        <property type="entry name" value="CRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CRIC" evidence="7">
        <location>
            <begin position="78"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="208"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="460"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="562"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="749"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="802"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="836" mass="93166" checksum="DC2EAA8C41C3642B" modified="2014-04-16" version="1">MEPIASWSHDRVKDWLKGLEPSLQQYPFEEWHLTGQDLLQLSYKDLEGLGVKKIGHQELLLEAVEKLCSLNYGMSGESMRSVTEKLRGMAHSLQMGIQSRWRVNTYNGQSVCKLPSETLVCVIELIVAVRGCFSWLNRYLCSQPSDYIPIKDIISHCTELSCIVHKDINAYEKEKDIISICRKLVAICDDVLSSCPENLLNHTTSLESVDLVPASPGDNLGIEITSTGSSLHYVTGTAAESPAEVCEKILAGDEVIQVNNQVVVGWTRRNLVKKLQENPHGVTLVLKKVPVSVAHQERAAKPPPSPKDPEVRDPSSVLQRVAASVRTLSFKAAVQEEEVKEQKALESELGDRNSSPECVFYSSHPSQGGEPWSNEGVAPLNQTERADWDTPKSASGPGDSPVLPDSPQLMPKEASRASVTSRSSCPEMAGQSQQDEKRPKTKGTLTAMSRRRVSCRELGRADCDGWLWKKKKDSGVFVAQKWQRFWFVLKGASLYWYASQQEEKAEGLIHISAYSIESAGEHKRKYVFKVCHERFQTFFFAADNVSDMSKWINYLITAIQKHKKDHNNPPNSEEDCYSETEPEDEGPRSPGASRRKAGTKKPSDRAVNTLPRMKVKDKQPKKPILTQGVMTTASDKSQTAPAPEDEMGILFHRLKEGGVSLIGNEQLLTRDHFRKSFIKRCKNPVINEKIHTLRMLQSTLKAKESELVIINKILDDPRLTSEKFREWKQRNGELCQEIAKLAKAKRAVKGATPGEVKGAGEDESGQESGLDRHCPSSNQRDALLDASFRVRLTQGEQLVDSEPAGLDVESCPMETDPADTLPSGTANVSSDNYFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME98</accession>
    <name>W5ME98_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol-glycan biosynthesis class X protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Stabilizing subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PIGX family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME98"/>
    <dbReference type="FunCoup" id="W5ME98">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006707"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006715.1">
        <property type="protein sequence ID" value="ENSLOCP00000006707.1"/>
        <property type="gene ID" value="ENSLOCG00000005556.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S32M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017679"/>
    <dbReference type="HOGENOM" id="CLU_076787_1_0_1"/>
    <dbReference type="InParanoid" id="W5ME98"/>
    <dbReference type="OMA" id="ALSKYMW"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005556">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025093018" description="Phosphatidylinositol-glycan biosynthesis class X protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="262" mass="28899" checksum="D74CA0D2B526127B" modified="2014-04-16" version="1">VYAMRIVYLIAFILAPALLKADLQSGEERCGFTREWLESLTVSRELRKNGFHRELVTSVCCGLGSPKNLHFLLVERLPKGIYIDPYQLTSEQHDTGLQVLLDTEIDVEAPAYVSSAFPALVYPTRDPASPVCLKAVVPIHGRYHRPSDSGKRTAPIDIENPHLLLRADSCESSGDLPPNFIPYTLTDAPCTALNHSMCRWLQIHSAQEPGPLRLEIPVGDMRLVGLVSAATLLVTLLSCGYLAGAVWRNEFLQSRPPVSQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEB1</accession>
    <name>W5MEB1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Microtubule crosslinking factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MEB1">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006720"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006728.1">
        <property type="protein sequence ID" value="ENSLOCP00000006720.1"/>
        <property type="gene ID" value="ENSLOCG00000005565.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182982"/>
    <dbReference type="HOGENOM" id="CLU_002595_0_0_1"/>
    <dbReference type="InParanoid" id="W5MEB1"/>
    <dbReference type="OMA" id="DDMKDHS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005565">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027882">
        <property type="entry name" value="SOGA1/2-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027881">
        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF1">
        <property type="entry name" value="PROTEIN SOGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
        <property type="entry name" value="SOGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14818">
        <property type="entry name" value="SOGA1-2-like_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="459"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="583"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA 1/2-like coiled-coil" evidence="8">
        <location>
            <begin position="1183"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="866"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1059"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1269"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1540"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="288"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="387"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="529"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="922"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="894"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1287"/>
            <end position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1753" mass="196126" checksum="7C3D9584C412C2DA" modified="2014-04-16" version="1">MTKTKADATLPNHFVLLKERRQRQKMDSGQGNDDICQQTQPPPPTFEEQPTSQPPQTSGKKKVNRAPSPARPKDVPGWSLAKIRSGSGSGTPTLSVKTGGIHLGSRISRRCPGSGKELKPEKSRQPGKSTPSVSGSHKSGNKSAKSARRKISDASTASDDLSKDSGCAPGKLSPSDSSSELSDCASEGNKLSTDALSSDTETSSRGGGADGEKPGTGQANGISDKANRGDQTGKNPLDKDRLSLGVETGEESISPGDERSVGSFDSRLHVSTSLAFSDLTEEFMDGMQEEFVREIEELRSENDYLKDEIEELRSEMLEMRDVYMEEDVYQLQELRQQLDQADKTCRILQYRLRKAERRSLRVAQTGQVDGELIRVLEQDVKVAKDVSIRLHNELDAVEKKRSRLEQENEDLRQKLLDLEVAKQVLQAEMDKVKENSLKKRGARLANKTDKKPSPQFQEDSADLKCQLHFAKEESALMCKKLTKMAKDNDSMKEELVKYRSLYGDLDSTLSVEEVADSPHTREAELKVHLKLVEEEANLLSRRIVELEVENRGLRAEMDEMKSQELPGHLVTGTSSYGDSRESMVELKRHLQFVEEEADLLRRSLLEMEEQNKLLMNELNKYKSEHDLDATMSEDSCSVMSEPSQEELLNAKLQIGELSGKVKKLQYENRVLLSNLQRCDLASYQSTRLSMETDAEAGDSAECVPSQIRREGPIGGENDSREDQEKLIRRNSHIDNYEGSEFFKSKDYDALLRIKEQAGLVSKAIDLMILDSNGFSSYPKICPSIDTPEQALNESLDKSEASSDLKLVSALNNRLSALQRELATFMDKVEHLGYSSREQIEGASPLPLLSESGSSGTMRCLREGEINPRFNHPHYSSPKPPVKELEAHLSGAEDNENRDSNKDLENYTPDDKESDIIKHAAKIRELQSLLSEAKEGIRGFQEQLSQEQEAHRQETESCSQKLAQLKEEHQKALIRRDFELQSLNLQTRLQQKFWSQERNLMIQESQQLKQNLLLLNLKLRWFLKQWRLGKKLDSEGKDILEVNSVKDLYMLIEEEGLTAHQLDNKTEDQSQSPTARADNLSEKNYKQVPGGLDGALADLKEALRDLSTELQEERRGSEELTQQFAKAKAAWEVERSELKCHITQLECKAGKPGLDKELPDLKAALKKEREEHQHLLADSYAAVMDLTKQLQISEKNWSREKVELLERFNQERGQWEQRLRDAQSKISQAIHTGTRHKECATACVALNLCFSRTKSVSSISEFESLLDSSPFVPSQDNGDASKGRGIQPSAQPISNNLSHLGELNKKNWKYLTNEMALYEKADPFKTWDCPTMTNSFAGLDLTQSCIQRSYTAPDKTGIRIYYSPPVVRRMEGSLAKEKQEEKTMAEPGYLFTTTKPRERGESENPSDNTFNRWLCNFSKQHRDLLENNSKAATTAGLPSPFHNLEISGNLSDDMKEMTNCVRQAIRSSSLERKCKDTASQTVVVTSTGTQTAQFVSIGLQTEGPRTGLHAKSWSPRSSSLMSARSRQISTSLEKVHSRIERPCCSPKYGSPKLQRKVSTSSSKLDTSKDRSLWNLHHRGQNGSAWARSTTTRDSPVLSGLNDGLSSLFSVVEHNGSTESLWKGIPQESKPKAEVPKYGIVHEFFRNVCGRVQTPMPVAENSPAATVGSASPDNVSKIVNKRFLKQSLRDEQAQGNKDTAARDTNMSSSALEDAACDCTSQSLTSCFARPSRSAARHSLAQCKMRSLEAGAAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF44</accession>
    <name>W5MF44_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Eukaryotic translation initiation factor 4E type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Eukaryotic translation initiation factor 4E-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">eIF4E-like protein 4E-LP</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. In association with GIGYF2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide. GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3. Does not interact with eIF4G (EIF4G1, EIF4G2 or EIF4G3). Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598. Interacts with GIGYF2 (via the 4EHP-binding motif); the interaction is direct. Interacts with EIF4ENIF1/4E-T (via YXXXXLphi motif); increasing affinity for the 7-methylguanosine-containing mRNA cap.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MF44"/>
    <dbReference type="FunCoup" id="W5MF44">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007003"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007011.1">
        <property type="protein sequence ID" value="ENSLOCP00000007003.1"/>
        <property type="gene ID" value="ENSLOCG00000005798.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154694"/>
    <dbReference type="HOGENOM" id="CLU_043552_3_3_1"/>
    <dbReference type="InParanoid" id="W5MF44"/>
    <dbReference type="OMA" id="VWNKTAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005798">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E type 2 isoformX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF17">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 19" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 19" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="191" mass="21928" checksum="76C75E9516E3B1A9" modified="2014-04-16" version="1">VVPGAGEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGSFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKSGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKAWEEFHGLVNTSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFD6</accession>
    <name>W5MFD6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine protease 56</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFD6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007103.1">
        <property type="protein sequence ID" value="ENSLOCP00000007095.1"/>
        <property type="gene ID" value="ENSLOCG00000005875.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157183"/>
    <dbReference type="HOGENOM" id="CLU_034171_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFD6"/>
    <dbReference type="OMA" id="VMEIQHR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005875">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF10">
        <property type="entry name" value="SERINE PROTEASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046489265" evidence="6">
        <location>
            <begin position="18"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="106"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="578" mass="63459" checksum="1388277B9E7F0447" modified="2025-10-08" version="2">MRMLLVYLLQILDCSFAAPTGREVYRLPQSTLQALSERGTVVLEAALASALSALELAVSERSRAERGCKGCPPCLFQDCGQRTGPCGVCGRKMVAVQNVTQPRSRIVGGFPAAPGAWPWLVNLRLEGELMCGGVLVDSTWVLTAAHCFTGSRSENSWTAVVGEFDITKTDAEEQVMKVNRIISHPKFNKKTFNNDIALVELTSPVVLSDRVTPVCLPSGEEPAVGTPCSVAGWGSLYEDGPSADVVMEAKVPILSQNTCKSALGKELVTNTMFCAGYLSGGIDSCQGDSGGPLTCQDPVSGRFQLYGITSWGDGCGVRGKPGVYTRVTAFSDWVRSEIQKSLGSREPTCPELLRTSELSEEQQRSEFSALCRFYVNSCSPLMGTAACTHLAEDKCRTRYKKCQLRSFLQTLLDLLQRADVYIREKAEAPAVHKLEREYRSLIELLHSKPAIESSFTKQMGKLPLQMWINNVELLRDQRYCSDVAALCPGESESAQHVNMVREAYRWILGIPSKNLCMTFQEVLVDIASKNEKGLYEARVRATVGGRPFTFYSLVGLESESFYRSMPRVIALALDALKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFS3</accession>
    <name>W5MFS3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Si:dkey-22o22.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017941">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MFS3">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007232"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007240.1">
        <property type="protein sequence ID" value="ENSLOCP00000007232.1"/>
        <property type="gene ID" value="ENSLOCG00000005972.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686834"/>
    <dbReference type="KEGG" id="loc:102686834"/>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164400"/>
    <dbReference type="HOGENOM" id="CLU_000347_1_0_1"/>
    <dbReference type="InParanoid" id="W5MFS3"/>
    <dbReference type="OMA" id="PYHTSQK"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005972">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000007">
        <property type="entry name" value="Cadherin 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000659">
        <property type="entry name" value="Crumbs cell polarity complex component 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000562">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000125">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000136">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000157">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000204">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000232">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000040">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000119">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000299">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000035">
        <property type="entry name" value="Protocadherin Fat 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000404">
        <property type="entry name" value="Si:ch211-186j3.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000804">
        <property type="entry name" value="Si:ch211-186j3.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000215">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000196">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000224">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000230">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000234">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056370">
        <property type="entry name" value="Shg-like_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF432">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24811">
        <property type="entry name" value="Ig_Shg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="2653"/>
            <end position="2674"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="31"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="140"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="445"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="555"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="648"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="758"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="864"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="964"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1076"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1191"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1293"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1411"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1514"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1633"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="23">
        <location>
            <begin position="1727"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="2091"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="21">
        <location>
            <begin position="2131"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="2335"/>
            <end position="2374"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="21">
        <location>
            <begin position="2377"/>
            <end position="2554"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="2599"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="2731"/>
            <end position="2759"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="2120"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="2364"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="2626"/>
            <end position="2635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2864" mass="316295" checksum="1FDB19F2CC9CB670" modified="2014-04-16" version="1">MMVRRKTVDLSENLTTLMIFIILSVELSNAALTVYYGQVEENAPAGTQVEGLLIPVWKKCPGLIRKSVLDFTLEGENASDFHPDYHGEGRLLLKTTKTLDRELKAVYDLTATPPVSVCSNTTVRIEIKVLDLNDNRPRFIKKSQILEIDELTPIGAELMQLSALDRDSGENGRIAYYAFPPNELLFVVPKNGRVILVKSLIGRKTITLCAFSKDNGNIALQSEPVTFQFKVRRPDVEGNSCEVRKPRAFSEEMFYTVSVSEDVKVGDMIFSVPDLKFEKKWFEVISEADSPVQIERDSGRLYLAQKLKSAADVIIKIQNVEGDDWYLCRLTLTVPSLADLEWTMFPFPYLAAVAPDAAKGTVVYQLSVHHRGSVGSSEAAQFTLIEGSKEYFEVDWDSGEIRTTGWPLTPQSVYVLTVQAVDRLGRRGPYASISILAGFRPPQFTNMSYTVFAPENTAAGQAVAVMKAISFQRKPLSYTLLFNPSSLFSINQESGVLSLTRSVDYESEHHLHHLQVGAMEQGTGLSTVTEVVVHISDENDCTPEFLHSIYSRDNIPETIPPGTSLLQVLARDCDTGQNSEISFFIQSNDFDITPQGVISSRQQLDYERVNHMYEFVVMAVDKGYPSRTGTASVRIRMANVNDEAPVFSQAVYKSFLSEDAGPDTLVAIVHAKDPDGDAVTYAITGGNEDSNFELDSQKGIIKLRKSPSPKLRGPQYILNITATDDNSAGGPQALSSSAQVIVGINDINNNKPIFEECHTYSVNAFVQENQPPGTFVLRVDAYDADTGVNGEVKYGIMHRDGTTSGFVINPDTGDISTAMSFDREKQKEYTLSVTATDMAQEPLIGICQITVMIADENDNDPKFENSRYQYFLREDTPVGTSFLRAAAHDDDQGPNAAITYSLSYQQPAYLHINPNTGWIYVNHPISQTSQISQQIIAKDGGNRSSSVELTVIITNVHNQPPQWEQDQYWVTIPENTVRDTKIVMIKATSPLGDPRVTYNLEEGQVPETNMPVRFYIKPNRQEGSASILVAEPLDYETTRFFTLRVRVQNVAAVPLASFTTVYVNVTDVNDNVPFFMSSTYEATVQEGAEIGTSVAQVSATDLDSGLHGMINYIILKDHSGDSQVFSINPQTGVIYTRASFDREQKSSYLIEVQSQDSSESARPGQRGQPNTDTAYVRVFVMDVNDNAPAFPQSVYEVSVEEDKDVGFIVLTVTANDEDEGANAKLRYQITSGNSKGTFDVEPEVGTIFIAQRLNYEQNQHYELRLVASDGKWENQTLVIINVINQNDEAPIFSQSEYHSSVMEELTKLPVLVLQVSATDPDQEADQSALRYSLHGQGADSEFTIEALTGKIYAQKKLDREGRSVWQFLVLATDENGAGLTGFADVIVEVQDVNDNSPVFLCAIDSCFVGYVLENSPADTSIMEMTATDLDDPKANKNAILTYRIVQNVRNEINLNLFSINPFTGTIYTVLRSLDREVEDKYLVVVEARDGGGLTGTGTATIIVSDINDHPPVFTQRVYMASVTESLDINSEVLVMSATDGDEGENAMMTFSIVGGDEDRKFFIETDKMNRRGTIRLKKKIDFEKPHERTFNLTVKVEDMDFFSLAYCVIQVEDSNDHAPVFFPQFYEAETLFEDVPVGTIIAHVTAVDLDSGQNGRFTYSIEPESDPYEQFLVDKAGSVLVADSLDREKIAQHRLLLHATDMGNPSLTGTAIVLVSVQDVNDNGPDFEVPYHPIVWENTGAPQVVQMNDSSLLLYAKDNDTPPNGAPFSFQLLMESPGVSSFNLTDLKNGSALITALQTFDREEQKQYCLPILMTDSGSPPMSATNTLTITIGDRNDHPHVAGHIEFIIYSYEGILPTTVLGQVQSPDLDDWNEKVYRFEGKPSRYFILNPNSGQLSIQEGTPSGSYSLQVRVADKTWPDVTSTVEVTVKDLKEEALQNAGSVRITNSTVEEFVSAPEGKESRYFRLKSLLSEIIPADLDNIHIFSVASSRLRPSEVNVWFAAHGSRYYKAEKLNGIVAAYKSKLEAAMGVTILQVGVDECAYSDCSHATGCSSIISFSNTPTALNSGNVSLVSVTASVNTQCTCSARERLPLSCSSYPHGPCLNGGTCIDSDLGYRCKCPPMFDGPECQQTKHTFHGHGYAWFTPIKPCFQSHISLEFITETANGLLFYNGPIAQAQPGDNEDFIALELKNGVPSLSVSYGSGTLQLQLPSTAAVTDRRWHRIDIISDGKSVQLILDRCSGALVNEFEGFVVEVLETDQTLCKATGETPGKERFLDVNQPLQLGGMKESFPFRRTQLHFTGFVGCVRNLIVDSQVYDLGCPAESLNSAPGCALTDGVCLTAGGPSCGIHGKCRGEWGSFSCDCHPGYSGHKCDKALPEWSFEKDSVLRYQLRGGGSARRTHAQLLLRTRTPSGTLLSMTSRDSNEYIVMEMVDGHFGVRANLGDGDHYLQLPALRIDNGQWVLLSLDRHDNVFTLRLDQGGGAREITAMLGHKREIIVHPSSVLLGNNLSDYQHSDFQGCMQDVRLNGHSLPLDGKSTEFHTVLERRGVLPGCHSDACSSKPCRSPLYCVDLWRKHECRCPAGKMTVEDGISGLLHCVPSPCGPSVCRNGGTCVAQSPRSFTCVCMEGYKGQWCEVSLVKAGRMAVLSPSSILAISMCLLVFFAVLVAVTVWNQKGSPSKFQKRGVYHIPAEHESWEDIRENILNYNEEGGGEQDQNAYDITELKRPLCSSLSQSSSGTTAPLLKSSQGSQEEVHPGTMASYHQNATGVASGPTDFKSYVSRIIWEADNDDQSFPMDSFQIYRIEGLDSPVGSLSTLVSEGEEDFTYDCLQEWGSKFEMLSELYRRPEFVLTYRDIMQKENPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGN5</accession>
    <name>W5MGN5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Perforin 1.2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGN5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007544"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007552.1">
        <property type="protein sequence ID" value="ENSLOCP00000007544.1"/>
        <property type="gene ID" value="ENSLOCG00000006250.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RQWS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063725"/>
    <dbReference type="HOGENOM" id="CLU_039516_2_0_1"/>
    <dbReference type="InParanoid" id="W5MGN5"/>
    <dbReference type="OMA" id="RCEEYIP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006250">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF5">
        <property type="entry name" value="PERFORIN 1.2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866208" description="Perforin 1.2" evidence="8">
        <location>
            <begin position="26"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="10">
        <location>
            <begin position="31"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="398"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="66524" checksum="3E58B60EB3FC163D" modified="2014-04-16" version="1">VPYRMWQFLLLFWALSLSSRPGTFARDFVGTPAECKTAEFVPGYNLAGEGFDIVTMQRKGAYVVNMEDWKKPKGSCNLRQNRYLKGKKQKIPLAIVDWRTLPNCKRQLSSTIYESSESLVNDSTSSVENNWKTGLDLNVNPNVQVGATLGGTHSRDATYAMQKSKQDKYSFTSHEVHCKFYRYRLVTKPPVHTEFLESLKMLPRSYTSDTKEQYRGLINIYGTHFIRQVSLGGKVKSITSIKSCQATMNGLTDTAVKDCLDVEASATVGMVATVKTEFHRCESLKKKMNLAQSFGSMFSDRQTEITGGQIDSADLFFSGASDPSAYKQWLETLKTIPDVVSYSLEPLHKLMPPNDPRARQLQRALGDYVLENALLKKCSEPCQVGDRVSARDCCACVCKGNNNVKSNCCPAGKGLAQLQVYGLEAKQLYGDRGTKTDGSVKVTFGEKSRRTEVINENDNPVWPEKFEFGNIVINMATKLQVEVFDADSRWNSDLLGECSFALKSGTNKNVCYFKHGTFFFSYTVECAPSLGGPQCNEYIPSPMSPSLATKFHSRNGVLASESWVPEFRKNQAKSRNLKCNSSASHEEEIGYSMSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH63</accession>
    <name>W5MH63_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Adaptor related protein complex 1 subunit mu 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634903.2">
        <property type="nucleotide sequence ID" value="XM_006634840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH63"/>
    <dbReference type="FunCoup" id="W5MH63">
        <property type="interactions" value="1694"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007722"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007731.1">
        <property type="protein sequence ID" value="ENSLOCP00000007722.1"/>
        <property type="gene ID" value="ENSLOCG00000006392.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695738"/>
    <dbReference type="KEGG" id="loc:102695738"/>
    <dbReference type="CTD" id="445244"/>
    <dbReference type="eggNOG" id="KOG0937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166725"/>
    <dbReference type="HOGENOM" id="CLU_026996_0_0_1"/>
    <dbReference type="InParanoid" id="W5MH63"/>
    <dbReference type="OMA" id="DMNEIDY"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006392">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09258">
        <property type="entry name" value="AP-1_Mu1A_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000002">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000006">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="12">
        <location>
            <begin position="202"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="52219" checksum="516BA709B3841BE0" modified="2014-04-16" version="1">MDINVNWNLVHISDTHPENNSYPRSGLLPTSYRRMSASAVFILDLKGKVLICRNYMGDMDMNEIDHFMPILMKKEEDADISPLLTHGASHFLWIKHSNLYLVAITKKNANAALVFAFLYKIVEVFKEYFKELEEESIRDNFVTVYELMDEVMDFGFPQTTDSKILQEYITQQGHKLEVGAPRPPATVTNAVSWRSEGIKYRKNEVFIDVIESVNLLVSANGSVLRSEIVGSVKLKVVLSGMPELRLGLNDKVLFEITGRDKSKTVELEDVKFHQCVRLSRFENDRTISFIPPDGECELMAYRLHTPVKPLIWIESVIEKYSHSRVEIMVKARSQFKQRSTANNVAIMVPVPSDADSPKFKTSTGSAKWVPEKNMVVWTIKSFPGGKEYLMRAHFGLPSVESEELEGKPPITVTFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITQSGDYQLRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH79</accession>
    <name>W5MH79_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01003067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627871.1">
        <property type="nucleotide sequence ID" value="XM_006627808.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH79"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007738"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007747.1">
        <property type="protein sequence ID" value="ENSLOCP00000007738.1"/>
        <property type="gene ID" value="ENSLOCG00000006405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691466"/>
    <dbReference type="KEGG" id="loc:102691466"/>
    <dbReference type="CTD" id="6188"/>
    <dbReference type="eggNOG" id="KOG3181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008610"/>
    <dbReference type="HOGENOM" id="CLU_058591_2_1_1"/>
    <dbReference type="InParanoid" id="W5MH79"/>
    <dbReference type="OMA" id="NKKKWMI"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006405">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000005">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 17" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9 16" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="19">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="245" mass="26821" checksum="8616D11DDF539F29" modified="2014-04-16" version="1">MAAQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPENSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEVLPTTPISEQKGAKPEAPVMPQAGPVATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHA0</accession>
    <name>W5MHA0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein Hox-B1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHA0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007759"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007768.1">
        <property type="protein sequence ID" value="ENSLOCP00000007759.1"/>
        <property type="gene ID" value="ENSLOCG00000006423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687482"/>
    <dbReference type="KEGG" id="loc:102687482"/>
    <dbReference type="CTD" id="58046"/>
    <dbReference type="eggNOG" id="KOG0489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157315"/>
    <dbReference type="HOGENOM" id="CLU_058839_1_0_1"/>
    <dbReference type="InParanoid" id="W5MHA0"/>
    <dbReference type="OMA" id="QELICAG"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006423">
        <property type="expression patterns" value="Expressed in embryo and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="230"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="232"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="360" mass="37426" checksum="05551C2057472FEB" modified="2014-04-16" version="1">MNSYQEFACGGDPGAQAAGGYPAAEVRLQHFDQRPLPKAGCGGEGRYVVGAGFPSPFSPDKPSPPPARDFSTTCAFHAGSLQVALGPGTPARAAFSHGQGYPSAPAYLRHPYRNPPDLDYYSGAAYTSSADLPASFSGLLSGLGPLDQHPQACAQEGAEPALAGGASPRPGGGGDGGPCSSSKTFDWMRVKRKAPRAAKRLPGGCAVSYLALGLGPSGGDPSGLAQGCLQGNGAPRTNFSTKQLTELEKEFHFNKYLTRARRVEIATALQLSENQVKIWFQNRRMKQKKREREGLVLGALPSPCSSQEDSPSDKSDLCSSPAPSPPPPPPPRARTVTGSPAASSQPEVCAQVPVATPDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHF2</accession>
    <name>W5MHF2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Hemopexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the hemopexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020368">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHF2"/>
    <dbReference type="FunCoup" id="W5MHF2">
        <property type="interactions" value="1405"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007811"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007821.1">
        <property type="protein sequence ID" value="ENSLOCP00000007811.1"/>
        <property type="gene ID" value="ENSLOCG00000006464.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009178"/>
    <dbReference type="HOGENOM" id="CLU_061713_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHF2"/>
    <dbReference type="OMA" id="CSSAMRW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006464">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000009">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051298">
        <property type="entry name" value="Heme_transport/Cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016358">
        <property type="entry name" value="Hemopexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917:SF9">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917">
        <property type="entry name" value="HEMOPEXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002551">
        <property type="entry name" value="Hemopexin_chordata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 13 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867787" description="Hemopexin" evidence="19">
        <location>
            <begin position="17"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="162"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="207"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="318"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="16">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="61"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="171"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="374"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002551-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR002551-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR002551-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="52843" checksum="B2526D2490B2CB43" modified="2014-04-16" version="1">IMRLLFQALCLCLALAYHHEDHHPGKPGVHQGAHHDGHHGPPHDDHHGPPHDDHHKHPDRCAGIEFDAITPDEKGNTFFFKGDHLWKGFTGKAQFLNESFHELDDHHHLDHIDAAFRMHDEEDPEDHDHIFFFLNDKVYSYYNHTLEAGFPKDIHDEFPGIPDDLDAAVECPKGECQTNSVLFFKGSHVYHHDLKTHAVKEKNWPHLGNCTAAFRWLERYYCFHGHSFTKFNPVTGEVKGDYPKDARNYFMRCPGFGHGKNDSKRDHIERCSKLPFDEFTSDDEGRSYAFRGAYFLRLDTARDGWHAWPIDSAWKEVHSDVDAVFSWDHKLYIIKDGKVYIYKTGQHYTLVEGYPKTLKEELGVEGEVDAAFVCAKEHVVHIIQGSKMLDVDLTATPRTVVKEWTLPYKHIDGAMCGADKVRVFVGPEYFEYESPQLLALGRIKPPPHKISVDLLKCED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHR9</accession>
    <name>W5MHR9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">EF-hand domain family, member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHR9"/>
    <dbReference type="FunCoup" id="W5MHR9">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007928"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007938.1">
        <property type="protein sequence ID" value="ENSLOCP00000007928.1"/>
        <property type="gene ID" value="ENSLOCG00000006567.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063528"/>
    <dbReference type="HOGENOM" id="CLU_017580_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHR9"/>
    <dbReference type="OMA" id="DCIRPIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006567">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="584" mass="65575" checksum="EABFE584AD07F12D" modified="2014-04-16" version="1">KMLEREAIYAAKYKDRFPSIKSAGKLIPTGERAETCLKEITPRPVTPPVVKKFLNTTRPDPGAVRVFYGKAGDPDVASSLVHGVSTRPSLTAALLTNPSPKTLFQQKLQELREAAYSSHQKAPLGRTCNQPLGLPNGMDIEKTTFGSKTLQSVNAGEIIHPPKSHEQVEKELQEGHHLYIRSHNAYFVGEPVNRKYDWTKYSKSSRFGIATPHYNDGRNVAKSLQWLHHLQMERGAKLVTKQSDDFRERTQPQIGKVHDPIADTMNVPLNHTYGIMVRPDEFGVGYLLHHTASLDFLRGKDRKIAILNAVRQHLKKANFHNFSSLLEAFRHYDKKGNGVICKEDIQDVCRQFNLDLHPQLLESLIAFCDYDNDGRINFLEFSNFLNWKDKMPIGELEEKILTKGRNKADSAPPHSQKTLKEGIREEALAKEGDLVPAEGGIVKTPKTLPRPKTVPDNYTTSSSMVSAVVGGLSTVGYRVYGVPAVRTDLAAPRIKRVSDRTNYGDQSNAGGLIHPSLYSLKGVHEKDFFISRPKEEIACIFRNVGMDISEETFHEVWNLAAMKHPRGQVCVETFRNVLDEIQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI31</accession>
    <name>W5MI31_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI31"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008040"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008050.1">
        <property type="protein sequence ID" value="ENSLOCP00000008040.1"/>
        <property type="gene ID" value="ENSLOCG00000006658.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173967"/>
    <dbReference type="HOGENOM" id="CLU_085240_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI31"/>
    <dbReference type="OMA" id="RANCLAQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006658">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053040">
        <property type="entry name" value="LRR-containing_protein_71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46984">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46984:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="285" mass="31179" checksum="FBE90A5DFC50A702" modified="2014-04-16" version="1">MGKKLEKAPKEKIDRGHSGTEEDSSKSTGLNILEKSAVQSIEDYQCTGNLEADFPELCALVGMREIPPVTLRPRTGVTPTTDRSILDNSQSQLDGGGPPPAERFSCLKPRLQVELEGDDPRSVREVRVRGWKVGEQMARVFSRSLPNLSNLQSLHLWRVGLTQRTLTSLRNTLSLCLSLRTVLLEGTPIPEQCYHILIAEDSMLAHLSLRNNQIDEEGARLVGQALSTPRSANKSLLSLNLAFNRIGDTGAQHIAQGLRLNRTLLCLSLAHNHIGDPGASGLAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MID8</accession>
    <name>W5MID8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin conjugating enzyme E2 U</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MID8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008147"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008157.1">
        <property type="protein sequence ID" value="ENSLOCP00000008147.1"/>
        <property type="gene ID" value="ENSLOCG00000006744.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694031"/>
    <dbReference type="KEGG" id="loc:102694031"/>
    <dbReference type="eggNOG" id="KOG0419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162256"/>
    <dbReference type="HOGENOM" id="CLU_076373_0_0_1"/>
    <dbReference type="InParanoid" id="W5MID8"/>
    <dbReference type="OMA" id="SEDMMQW"/>
    <dbReference type="OrthoDB" id="9978460at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006744">
        <property type="expression patterns" value="Expressed in ovary and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ubiquitin conjugating enzyme E2 U"/>
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        <property type="entry name" value="UBQ-conjugating_AS"/>
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        <property type="entry name" value="UQ_con"/>
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        <property type="entry name" value="UBC-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBC_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="4"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="329" mass="37473" checksum="CB10D10CFF18453B" modified="2014-04-16" version="1">MHSRSYLLLEREYLEQQKANLFGIVAEPVNDNLLEWVAKVRGLKDSLWEGGTIQLSIQYSEDYNTVPPVVRFSTIPFHPNVNKDTGRPCISFLDNKDEWDSRFSLTSILLAVQVMLSNPVLENAVNLEAAEMLRTSPSLFRQVVLDCVRNSRQLESGHSIHEDLVMSPCFPGLEGAEIGMVHVRNVKRISFEEYLKTWSGIATSKASQNQKNPLLEGLLCDPYLHAMHYGLQGTGILEEALAQNEAFKTIMYGPAEKSKKSQITVDEKLARIHQMKRVYLLKRTPEAVTHLQSSRDTPCSLQREEQWEREVDNLVAWTNSLSLEELEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK72</accession>
    <name>W5MK72_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TOX high mobility group box family member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020892">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000008792.1">
        <property type="protein sequence ID" value="ENSLOCP00000008781.1"/>
        <property type="gene ID" value="ENSLOCG00000007257.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686093"/>
    <dbReference type="KEGG" id="loc:102686093"/>
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    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158043"/>
    <dbReference type="InParanoid" id="W5MK72"/>
    <dbReference type="OMA" id="HINNGHP"/>
    <dbReference type="OrthoDB" id="10027956at2759"/>
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        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007257">
        <property type="expression patterns" value="Expressed in ovary and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="AGAP000281-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781:SF3">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HIGHMOBLTY12"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="409"/>
            <end position="602"/>
        </location>
    </feature>
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        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
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        <location>
            <begin position="449"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="614" mass="67460" checksum="95A6D2E129E6055C" modified="2014-04-16" version="1">MDVRFYPSAGGNPIPGEPSNLDFPHCLGYYNYNKFGNNNNYMSMAEASSALLAASEQTFHTPSLGDEEFEIPPITPPPETESGMGIADMGSPFQGLPNPPSTQGARFTPQFPPQSLELPSITISRNLMDPEGAVLNNGLPVNISQSHVHHCHPNTSMVMRSVVNMSNPSAGMMSRNQLTTINQSQLSAQLGLNLGGPNMPHTSPSPPASKSATPSPSSSINEEDPEEGNRAIGEKRPAPDTGKKPKTPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPSATFGEVSKIVASMWDSLGEEQKQVYKSKTEAAKKEYLKALAAYRASLVSKAAAESAEAQTIRSVQQTLASTNLSPSLILNTPLSQHPSVSAAAQALQQALPRAIAPKPLQMRLSGSQIVTSVTVSPQNMPTNIPSPLISQIGGGMVGAPPAGVAQMSPPMQPPQPHQLQHLQQHQQQQQQQHLQQQHLQHQQLQQQQIHHQQIQQQMQHQHFQHHLQQQLQQHQHHLQQQQQHQQQQQQQQHHMQQQQMQLQHMQMQQLHQQQLQQLQQQQQQQQQPHPPQSSPPQHSPGTPQSVGGSVTLGSPQSTSQQQHQSQIQAHAQAQVLSQVSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLI0</accession>
    <name>W5MLI0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Matrilin 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLI0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009250.1">
        <property type="protein sequence ID" value="ENSLOCP00000009239.1"/>
        <property type="gene ID" value="ENSLOCG00000007617.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102698979"/>
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        <property type="component" value="Linkage group LG18"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005576">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="matrilin-2 isoform X1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036337">
        <property type="entry name" value="Matrilin_CC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019466">
        <property type="entry name" value="Matrilin_CC_trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Matrilin_ccoil"/>
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    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
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        <property type="match status" value="4"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Chicken cartilage matrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="EGF_3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013266209" evidence="10">
        <location>
            <begin position="16"/>
            <end position="615"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="209"/>
            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="291"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="380"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
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        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="615" mass="67751" checksum="6EAFA92D65661F34" modified="2014-04-16" version="1">MKLLLLLALVAFLEARPQRPADSKCKTGPIDLVFIIDSSRSVRPFEFETMRKFMIDIIHTLDVGANATRVGVVQYSSQVQNVFSLKSFTRKADMVKAINEIIPLAQGTMTGLAIQYAMSVAFSPEEGARRGVPSVAVIVTDGRPQDRVAEVAAQARDRKIEIYAVGVARADMTSLRAMASPPLKDHVFLVESFDLIHQFGLQFQDKLCGVDLCTEMDHGCQHICVSSPGSYHCECRAGYTLNTDSKTCSAIDLCAGGKHSCEQICVSSPGSYTCRCRAGFYLNQDKMTCTMIDYCSFGNHSCQHECVSVLNGYFCRCNEGYTLLDDGKTCQLEDLCNTVEHGCEHRCVSAPGSYHCVCPEGQLLQEDGKSCSTCRSANIDLVMVIDGSKSVRPQNFELVKQFVNQVVDSLDVSAHGTRVGLVQYSSRVRTEFPLSQYKSAEDIKNAVLKVEYMEKGTMTGLALKHLVENSFSEAEGARPPSKGIPRVGLVFTDGRSQDDITEWAKKAKDAGITMYAVGVGKALEEELREIASEPVDKHFFYTTDFTAINQIAENLKLNICAEESQGEIEVKDPCACESLVEFQQSTLSTLEQLTQKLAGMTARLEDLENQLLSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN61</accession>
    <name>W5MN61_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NAD(P)(+)--arginine ADP-ribosyltransferase</fullName>
            <ecNumber evidence="11">2.4.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mono(ADP-ribosyl)transferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19149"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:15087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142554"/>
            <dbReference type="EC" id="2.4.2.31"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Arg-specific ADP-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.31" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01027184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634213.1">
        <property type="nucleotide sequence ID" value="XM_006634150.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN61"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009831.1">
        <property type="protein sequence ID" value="ENSLOCP00000009820.1"/>
        <property type="gene ID" value="ENSLOCG00000008088.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689809"/>
    <dbReference type="KEGG" id="loc:102689809"/>
    <dbReference type="eggNOG" id="ENOG502SKQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234601"/>
    <dbReference type="HOGENOM" id="CLU_059744_2_0_1"/>
    <dbReference type="InParanoid" id="W5MN61"/>
    <dbReference type="OMA" id="NAAMEWR"/>
    <dbReference type="OrthoDB" id="423533at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008088">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106274">
        <property type="term" value="F:NAD+-protein-arginine ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.176.10:FF:000003">
        <property type="entry name" value="NAD(P)(+)--arginine ADP-ribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.176.10">
        <property type="entry name" value="Toxin ADP-ribosyltransferase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050999">
        <property type="entry name" value="ADP-ribosyltransferase_ARG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000768">
        <property type="entry name" value="ART"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339">
        <property type="entry name" value="ADP-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339:SF25">
        <property type="entry name" value="SECRETED EXOENZYME S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01129">
        <property type="entry name" value="ART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00970">
        <property type="entry name" value="RIBTRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TR_MART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005150873" description="NAD(P)(+)--arginine ADP-ribosyltransferase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="260" mass="29337" checksum="AFB0A2F46148868B" modified="2014-04-16" version="1">MEKFLLTVIAMVSAALPAEMRKIQFNSSDEIVLDMAPNSVDDSYADCTPQMLKNVLNNYLLKEQNSNSVFKNAWNSAEKHWQKHPTKLPSGMRKEHALAMLTYTKGGKNPIYEKFNAAVRRGAKSYKTDFSYKSLHFLLVNGLHLLKQQEGQPCRVTYRGTSKLFRVQKGSQVRFGSFASSSPDQNVANNFAKKSLFKIKTCLGADVMAFSSCPKEKEVLIPPHEKFEVTRVTKGRLQEIELISKGSQSNLMCALFRKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP53</accession>
    <name>W5MP53_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Si:ch1073-90m23.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP53"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010162"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010174.1">
        <property type="protein sequence ID" value="ENSLOCP00000010162.1"/>
        <property type="gene ID" value="ENSLOCG00000008359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3686">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166290"/>
    <dbReference type="HOGENOM" id="CLU_010739_3_0_1"/>
    <dbReference type="InParanoid" id="W5MP53"/>
    <dbReference type="OMA" id="SVDRFTC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008359">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF1">
        <property type="entry name" value="RAP1 GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="3">
        <location>
            <begin position="237"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="502" mass="55708" checksum="45086A6B20EBAED7" modified="2014-04-16" version="1">MEQDKARDQLFSRKRSFTFGAYGGSTGKTLILFHKERMTESMHGTSCVPSLFSPSLYFNALLFQASDLFEIIEKLQGSRLDEQRCEFPPPFKLQFLKIGGPLPLVLPPKAGGYWIEPPPCCEGAPLPPGPETEPGPCDIMDRDPSATLYRDSFRHKYHQSFTAVDPSLGSLILSARVDEEDSVHVILRMKECSLHGVFHLSLFPEFPSAGQLAKSINFLLCTASRFNLLYDPAPELIAAFDEHRISENFKFGVLYQREGQLSEEAILGNCEESEEFLDFLSIMGETVSLSGFTGYMPNIKIKLDNNINAILLTQRENGVPLYRPQRHSSHTEINTDSNTDPRGTPHTILTCFSCLSVNHSVHGAVLFPQVSVTAREDVPLFGPPLPDPPVFKERHRLREFLLIKLINAEISCYRAERFRRLEVRTRSSLLESLQSDLSARSCCMLGAAPPSASNGGPGRGESGGSGGGFIENFKRAIRVRSHSFDTLGGPRKVSGGSPQKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPU6</accession>
    <name>W5MPU6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Transient receptor potential cation channel, subfamily C, member 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPU6"/>
    <dbReference type="FunCoup" id="W5MPU6">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010405"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010419.1">
        <property type="protein sequence ID" value="ENSLOCP00000010405.1"/>
        <property type="gene ID" value="ENSLOCG00000008555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691071"/>
    <dbReference type="KEGG" id="loc:102691071"/>
    <dbReference type="CTD" id="102725536"/>
    <dbReference type="eggNOG" id="KOG3609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248594"/>
    <dbReference type="HOGENOM" id="CLU_005716_4_1_1"/>
    <dbReference type="InParanoid" id="W5MPU6"/>
    <dbReference type="OMA" id="QSPDKKC"/>
    <dbReference type="OrthoDB" id="2373987at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008555">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000023">
        <property type="entry name" value="short transient receptor potential channel 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000266">
        <property type="entry name" value="Transient receptor potential cation channel subfamily c member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF25">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08344">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01420">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="19">
        <location>
            <begin position="176"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="923"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="942"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="965" mass="110792" checksum="F2ABAD9D33BC05A9" modified="2014-04-16" version="1">MAQLYYKRTDNSSYRDRIPLRIVRAESELSPAEKAYLSAVEKGDYASVKQALEEAEIYFKININCIDPLGRTALLIAIENENLEIIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSDFTPDITPIILAAHTNNYEIIKLLVQKGVSVPQPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIALSSEDPFLTAFQLSWELQELSKVENEFKSEYEELSHQCKQFAKDLLDQTRSSRELEMILNFRDDTNLLEDENNNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAGKFLTCVFIGLMFPVFSIFYLIAPKSRYGLFIRKPFIKFICHTASYLTFLFLLLLASQHVVKTEPNRQGPAPTTVEWMILPWVLGFIWTEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATISLKIVAYVKYSDCKSRQQWEMWHPTLVAEAVFAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETEPSKENNYCKGIRCEKQNNAFSTLFETLQSLFWSIFGLISLYVTNVNAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFEEGGTLPAPFNIIPSPKSVWYLIKWIKRHICKNARAKRPESLGSLGRRAAENLRINHQYQEVLRNLVKRYVAAMIRDAKTEEGLTEENFKELKQDISSFRYEVLGLMKGNKSSMPGSKLNIGASNLPHSEEQFKYSPAVSDAQPKSKLNLYNVTTLLQQSSGSKASETYNGLINGSAILVSESSREKGQRKDFPKDISNFALFHRRQKVTNPGQNAGKIYSVSEEVDEYEPEEIARIAEVPEKAESSANKQLETGLKECDDELDKEVVHELSAYCDPSERSPEDTENPQEMPGEKHNESTEKNQEIEIMEHESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPZ0</accession>
    <name>W5MPZ0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPZ0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010463.1">
        <property type="protein sequence ID" value="ENSLOCP00000010449.1"/>
        <property type="gene ID" value="ENSLOCG00000008594.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163144"/>
    <dbReference type="HOGENOM" id="CLU_157356_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPZ0"/>
    <dbReference type="OMA" id="KTYLCFN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008594">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16255">
        <property type="entry name" value="EFh_parvalbumin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF12">
        <property type="entry name" value="PARVALBUMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="121" mass="13138" checksum="C3DAF582E9A7EB11" modified="2014-04-16" version="1">MAFSGLLREEDITAALQACQAAGSFDHKAFFAQVGLASDSAVAAEKAFRILDRDQSGFIEEEELKLFLQNFKADARVLTDAETKAFLAAADLDSDGKIGAQGLKFPRAPRAPMGPRHHRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQS3</accession>
    <name>W5MQS3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-binding cassette, sub-family G (WHITE), member 2c</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQS3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010732"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010747.1">
        <property type="protein sequence ID" value="ENSLOCP00000010732.1"/>
        <property type="gene ID" value="ENSLOCG00000008815.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165949"/>
    <dbReference type="HOGENOM" id="CLU_000604_57_8_1"/>
    <dbReference type="InParanoid" id="W5MQS3"/>
    <dbReference type="OMA" id="HYCVQEK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008815">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008514">
        <property type="term" value="F:organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000622">
        <property type="entry name" value="ATP-binding cassette sub-family G member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF49">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUB-FAMILY G MEMBER 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="29"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="671" mass="75133" checksum="7728B9E191A3B65D" modified="2014-04-16" version="1">PKSKMEGDSEPSTEAEEAAVYFQIPGPMITFQNISYCVSVKKNLLQRSAEEKEILRNVSGIMKPGMNAILGPTGSGKTSLLDVIAGRKDPRGLRSGQVLVDNQIMTSELRLRSAYVVQDDILMGTLTIKENLEFSANLRLPPREFSAEEKKLKVNAVIRELSLQDCADTKHIGTEFIRGVSGGERKRCSIGMELITSPSLLFLDEPTTGLDANTAFSIIRLLHRLSRNGKTIIFSIHQPRYSIFRQFDHLTLMNKGEIIFAGPAEKAVGYFEGLGYKCEPFNNPADFFLDITNGEIAPCTDMATPGLQSASGMLSGDKNPLALCYKESEFCSQVEEQLKQMGQVADPSATPVHIRASYATPFHYQLMVVCGRTVKNILRNPQTSYAQLALNIVFGILVGLIYYQIPNTIPEALQNRIGAFFFLVINMIFGNLSAVELFISERVLFIHENASGYYRTSVYFLSKVFADLIPNRIVPVFLFSGVSYYMMGLKNATDAFFLYCLTMSLVSLAAVSLAFLVSASVGSFAVANVLIALPYVFMMVFGGFLVNLNSMLSWLSWLKWISIFRYGLDALTINEMKGQVFTNNYTIVTGEMYMTQQSIDYSPWGFWQNEVALLGIMIVCMLMAYVQLRRINKWKNSNPQQKQNVKHMKTGLQSQMCLPLSWYVQHLRQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR71</accession>
    <name>W5MR71_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">General transcription factor IIA, 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003915">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR71"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010880"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010896.1">
        <property type="protein sequence ID" value="ENSLOCP00000010880.1"/>
        <property type="gene ID" value="ENSLOCG00000008922.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156726"/>
    <dbReference type="InParanoid" id="W5MR71"/>
    <dbReference type="OMA" id="VINDVXE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008922">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF7">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="378" mass="42191" checksum="C1207E45D70500B4" modified="2025-10-08" version="2">MFICVFRNPQPKLYRSVIEDVINDVRELFLDEGVDEQVLMELRTLWESKLMQSKAVEGFHPGQWTGLLSMHSGEHLYRSASCVRLSIKTPEREQRVLKLRVVHQNFIFFSFDVAPQQQVIVQDSKLLQHMNATGMSAAATAATLALPTGVSPYQQLITNQGQILQVVRAPNGAQYLIQPQQQVVLQQQVLPQMQPSGVQAPVIQQVLTPLPGGIPQQTGVIIQPQQIVIAGNKVPQNAQAAPMAAPAGQSTAQVPPQQQQQQQQQPAQPPMMLQVDGAGDTSSEEDEEEEDEYDEDEEEDKDKDGGEDGQVEEEPLNSGDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYVFSKAIGDAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTH9</accession>
    <name>W5MTH9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal protein of 97 kDa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat and IQ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTH9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011688"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011706.1">
        <property type="protein sequence ID" value="ENSLOCP00000011688.1"/>
        <property type="gene ID" value="ENSLOCG00000009560.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144283"/>
    <dbReference type="HOGENOM" id="CLU_016381_0_0_1"/>
    <dbReference type="InParanoid" id="W5MTH9"/>
    <dbReference type="OMA" id="AYWRGFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009560">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000165">
        <property type="entry name" value="Centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 97 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="635"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="530"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="718" mass="79160" checksum="884082C2A7B1ABEB" modified="2014-04-16" version="1">MLEIKGPVVDLSAQGIQRLEPSLPCPAGAHTLILDRNQIIKLEHLEKCEELLQLSVANNRLVRLMGVSKLVNLRVLNLPNNSIGYIEGLKDLGHLEWLNLAGNNIKVVEQLNSCVALKHLDLSDNNISQIGDLSRLSSLKTLLLHGNMITTLRTVPAHLPSGLTILSLAENEIRDLNEVSYLAPLPELEQLSIMSNPCVMATPSLPGFDYRPYIVSWCLSLKILDGYVVSQKEGLKAEWLYSQGKGRSYRPGQHIQLVQYLATVCPLTCTPALQSAEDAKLEKILNKQRLHQKQLLQQTVKGGPFSSSTLSRPSYLEVEQSPIYTPGGSEEREPITQVNTWLGRSVSSDHSYAADPVTRPPAADGEPLFLEDVQTDEDKLNCSLLCSESTFMPVASGMFPLSPTRQLQLESVEEPALNLTSCSDTREEQLVAKGQGAAAAGLPDSHMPSMGFLACTADCLQSGCQHLDQVHAFEECSDCSPVDGEQQGRTSGERSPDLDEAAVKIQAWWRGLWTRRFHPAAKEVRAEIRLRRMQEHIVFLSDELARLRKEREEERIQRLVQEEAVKFLWKQLQTFQLWQQSVNQQLQTFSQLGAPVSSTAFGLASSSLCESPSQDSLAPNADSPAARCCERSFPDSGFHSSGEPRGALATSHSAMDDSSSSATGDTLETVRPVADGKKDVADAGSRDSSRSEQDSSLLQQYLTSVRQLEEAEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTL4</accession>
    <name>W5MTL4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GDNF family receptor alpha 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027553">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027554">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027555">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027556">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTL4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011723"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011741.1">
        <property type="protein sequence ID" value="ENSLOCP00000011723.1"/>
        <property type="gene ID" value="ENSLOCG00000009594.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWSW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165282"/>
    <dbReference type="HOGENOM" id="CLU_040179_1_0_1"/>
    <dbReference type="InParanoid" id="W5MTL4"/>
    <dbReference type="OMA" id="CLENTIW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009594">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014707">
        <property type="term" value="P:branchiomeric skeletal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.110:FF:000001">
        <property type="entry name" value="GDNF family receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF15">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="11">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="11">
        <location>
            <begin position="123"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="11">
        <location>
            <begin position="213"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="405" mass="45001" checksum="423CD68B12B04CA0" modified="2014-04-16" version="1">DCVEAEELCASEPRCQTSYHVLEYCASEEAVAPLGPDARRECVAAEAALLHSPLLACKCQRGTRKEEQCLRVYWTVRYSQGYYEYEASPYEETEAQSRWSSPAVRMAPIVSGSTLPLDSQNQCLKAAQDCGLYEKCGALRSEYVLACTKRAPSSSHCNRQKCHRALRRFLERVPEEYVLAVLFCRCSTTLCGERRRKTIVPSCSYEEREKPNCLSLQGYCMRDDLCRARLADFQRHCQPSALSASGCVRESGAACLRSYIGLIGTIMTPNYISNSSDVVSQWCTCEGSGNQWQDCERVLRMFTSNSCLRNAITSLGSSAPRPVESTTPPPTRISLRVQQNNDIILPDLAKVEDEEEDDDEEFNVIPPYSEKATESNARSVAPGPHGGDVPKTLLLGAVLVLRGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU24</accession>
    <name>W5MU24_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Recombination signal binding protein for immunoglobulin kappa J region b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629715.2">
        <property type="nucleotide sequence ID" value="XM_006629652.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU24"/>
    <dbReference type="FunCoup" id="W5MU24">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011883"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011904.1">
        <property type="protein sequence ID" value="ENSLOCP00000011883.1"/>
        <property type="gene ID" value="ENSLOCG00000009731.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688769"/>
    <dbReference type="KEGG" id="loc:102688769"/>
    <dbReference type="CTD" id="563306"/>
    <dbReference type="eggNOG" id="KOG3743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005197"/>
    <dbReference type="InParanoid" id="W5MU24"/>
    <dbReference type="OMA" id="QRQDMPH"/>
    <dbReference type="OrthoDB" id="5600360at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009731">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002193">
        <property type="term" value="C:MAML1-RBP-Jkappa- ICN1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007221">
        <property type="term" value="P:positive regulation of transcription of Notch receptor target"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01176">
        <property type="entry name" value="IPT_RBP-Jkappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1450:FF:000001">
        <property type="entry name" value="Recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000003">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000074">
        <property type="entry name" value="Recombining binding protein suppressor of hairless, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="12">
        <location>
            <begin position="34"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="13">
        <location>
            <begin position="166"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="54389" checksum="66495A0BC9C4CC0D" modified="2014-04-16" version="1">MAPVVTGKFGERPQPQRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFYIHLLDDEESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVHAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCAESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCNAAGAILRANSSLMASSDTNTNSEGSYTSVTTNSTNVTSSAATVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU80</accession>
    <name>W5MU80_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64324"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:75121"/>
            <dbReference type="ChEBI" id="CHEBI:88768"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(7Z)-octadecenoyl-CoA + cholesterol = cholesteryl (7Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64328"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:152049"/>
            <dbReference type="ChEBI" id="CHEBI:152050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:64329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-hexadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64320"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:84323"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42796"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:41509"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42792"/>
            <dbReference type="ChEBI" id="CHEBI:3663"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:42793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42812"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:82750"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:42813"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Endoplasmic reticulum membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635320.2">
        <property type="nucleotide sequence ID" value="XM_006635257.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212087.1">
        <property type="nucleotide sequence ID" value="XM_015356601.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212089.1">
        <property type="nucleotide sequence ID" value="XM_015356603.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069050439.1">
        <property type="nucleotide sequence ID" value="XM_069194338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU80"/>
    <dbReference type="FunCoup" id="W5MU80">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011939"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011960.1">
        <property type="protein sequence ID" value="ENSLOCP00000011939.1"/>
        <property type="gene ID" value="ENSLOCG00000009767.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692222"/>
    <dbReference type="KEGG" id="loc:102692222"/>
    <dbReference type="CTD" id="6646"/>
    <dbReference type="eggNOG" id="KOG0380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183081"/>
    <dbReference type="HOGENOM" id="CLU_031845_1_0_1"/>
    <dbReference type="InParanoid" id="W5MU80"/>
    <dbReference type="OMA" id="SCMIEDV"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009767">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034736">
        <property type="term" value="F:cholesterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF6">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="361"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="557" mass="65132" checksum="17AD030F2432A99A" modified="2014-04-16" version="1">MTSEAGNSLRSRRVPTKVPGSVEMDGAVRGPEGEHRITSNGKMDVERIITKKLQLKMKAEHLKTDLMKQFDSQVNEFVDNLIEESASLESPTVHSLFPSVLCEKERNKLRHTRPPPDQGKLFVSRRSLLDELFEVNHIRTIYHMFIALLILFIISTLAVDFIDEGRLVLDFDLLVYAFGQLPLVVSTWLCMFFSAFVVPYVLFHQWARHYRTSSHRALGSLLAGALFLLFQVLVMGFLPTYVVLRNNLPPASRFIIILEQVRLIMKAHSFIRENVPRVLSSTREKNNSMSTPQVSQYLYFLFAPTLIYRDSYPRNPSIRWGYVATKFLQVLGCLFYAYYVFVRLCIPQFRNISLQPFKLRVMVLCVFNSILPGVLVLFLAFFAFLHCWLNAFAEMLRFADRMFYKDWWNSTSFANYYRTWNVVVHDWLYYYAYRDFLWMLGKRFRAAAMLSVFTVSAVVHEYALAICFGFFYPVLFCLFMCFGMLFNFILHDRRKGPIWNVIMWTSLFLGQGVLLCLYSQEWYAQRYCPVQNPTFLELLKPRSWTCHQQVSSAGRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUG3</accession>
    <name>W5MUG3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DHHA2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPase class C family. Prune subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUG3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012022"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012043.1">
        <property type="protein sequence ID" value="ENSLOCP00000012022.1"/>
        <property type="gene ID" value="ENSLOCG00000009837.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG2"/>
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    <dbReference type="Bgee" id="ENSLOCG00000009837">
        <property type="expression patterns" value="Expressed in mesonephros and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Prune homolog 2 with BCH domain"/>
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        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
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        <property type="entry name" value="DHH_sf"/>
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    </dbReference>
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        <property type="entry name" value="DHHA2_dom_sf"/>
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        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF49">
        <property type="entry name" value="DHHA2 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
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        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="2">
        <location>
            <begin position="202"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="625" mass="69058" checksum="DFA2C79AF84B2077" modified="2014-04-16" version="1">MLVSGSELPKVHAVLGNNSAEVDCVASILGYAFFLQQTEKTGDLCVPVLNSQRSELHIPDETRAFLRQSSISESSLIWRDEIDLRQLNTSGTLSLTLLSNNVLTSEDRALESSVVRVINPCERWGGSQELQELSSSTSLVAREILQEAPELLVQPLAHLLREALLFQSLKKSTKHGKLTPDHEELISELEKKLEDLGIQDRAINLKSPKLESQGTNMEQVLLKEIKELSDGDIKVAISTVYMNLEDYNNHPNLMEDLKVFCEQHGYEGLILVSSSSQEPYQRCQQVAVYSGNVELLNQICCELEEAQNSNLDMEPIDCLMDQIQLYEQRNTSVYIEQIIALIKEVLDRRQPLFVPNSRTSSTEAVAGSAPLSQGSSGFTDMYSSDAEPQSMSVHFIENSHDLNESAQPYGVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGETGGILQNPILGFPCTNSVSGSRKTQENKSQSASKNENFDLFSFDPLHSSSISPSANVADFSLDSNTLIFHEPLNILTNPQADGRPAQRVDDQITSTFSECGDLNQVEFYNEFVDEHLESEGNYLLGDGFSTTFQEQLIGDISDSKIPPTPMNSLVESSPLDDGLPKFFPEDVIEKINEMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV19</accession>
    <name>W5MV19_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 4E family member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020688">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630669.1">
        <property type="nucleotide sequence ID" value="XM_006630606.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV19"/>
    <dbReference type="FunCoup" id="W5MV19">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012228"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012249.1">
        <property type="protein sequence ID" value="ENSLOCP00000012228.1"/>
        <property type="gene ID" value="ENSLOCG00000010001.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695069"/>
    <dbReference type="KEGG" id="loc:102695069"/>
    <dbReference type="CTD" id="317649"/>
    <dbReference type="eggNOG" id="ENOG502QPP4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155865"/>
    <dbReference type="HOGENOM" id="CLU_043552_5_1_1"/>
    <dbReference type="InParanoid" id="W5MV19"/>
    <dbReference type="OMA" id="LPLQYHW"/>
    <dbReference type="OrthoDB" id="17977at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010001">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
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    </dbReference>
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        <property type="term" value="P:regulation of translation"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 4E family member 3"/>
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    </dbReference>
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        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
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        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF66">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="25237" checksum="AF546FC08A1F4374" modified="2014-04-16" version="1">MAVPAAPDLQMNAETQNSPANNPENNIHIDETELENITNNDEDGTALPLHSPWTFWLDKSLPGTTAAECESNLKKIYTVQTVQSFWSVYNNIPPVTNLPLRCSYHLMRGERRPLWEEESNAKGGVWKMKVPKESTAAVWKELLLATIGEQFADYCASEDEVVGVSVSVREREDVVQVWNVNASFASEANILGKIHELLPHISFKAVFYKPHEEHHAFEGGRSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVW2</accession>
    <name>W5MVW2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:ch211-163l21.7</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014294">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014295">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVW2"/>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012521"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012543.1">
        <property type="protein sequence ID" value="ENSLOCP00000012521.1"/>
        <property type="gene ID" value="ENSLOCG00000010228.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2J1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234625"/>
    <dbReference type="HOGENOM" id="CLU_133894_0_0_1"/>
    <dbReference type="InParanoid" id="W5MVW2"/>
    <dbReference type="OMA" id="QWINPKK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010228">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022179">
        <property type="entry name" value="CFAP276"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12494">
        <property type="entry name" value="DUF3695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="158" mass="18476" checksum="35FA70F29BCA9AA6" modified="2014-04-16" version="1">MPRSRDPFPYPKYENDDTFIGKPKNCQKVPYSKPTHLAQNEDPWNRLNARATLSSARREVLYFDPEVPQDRLDFQLKSLYDHHEEFLKDKNETLYQRETFTEDHGYSKDNNDSVLLRISVTPGVRCSPNSPFVFPESHHNASTNRGYSRKQDGGFYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MW24</accession>
    <name>W5MW24_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tetratricopeptide repeat domain 19</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TTC19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017483">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047213.1">
        <property type="nucleotide sequence ID" value="XM_069191112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MW24"/>
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        <property type="interactions" value="770"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012583"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012607.1">
        <property type="protein sequence ID" value="ENSLOCP00000012583.1"/>
        <property type="gene ID" value="ENSLOCG00000010277.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00390000009194"/>
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    <dbReference type="OrthoDB" id="5986190at2759"/>
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        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010277">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
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    </dbReference>
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        <property type="entry name" value="TTC19"/>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 19, MITOCHONDRIAL"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="272" mass="30617" checksum="0CDDCF4E6D124960" modified="2014-04-16" version="1">MQGELEAANRILHQAIRLAHQAKNTQAITYTYSMMANLALVQGQFPEAEKLFKAAMSFLLASGAQEDDNAVIEMSLKLASIYASQNKPELAEHGFEFCAESLEAKIEKQKDLPEGTLPEEERSNTRLLLGLCLDSYARYLTTKHQLDQAHQKYLRALQICREEQGEAHPQTVVLMNDLATVLDLQGRHQEAMEQMSQALELAKKAEHPDQHVLLCNMAGILLHQGQLVQARHVYREALLLAQATGDSEAMQQIRDGLEKLSNRERDEERETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWV9</accession>
    <name>W5MWV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromosome 3 open reading frame 38</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012868"/>
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        <property type="protein sequence ID" value="ENSLOCP00000012868.1"/>
        <property type="gene ID" value="ENSLOCG00000010497.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RKN5">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000367"/>
    <dbReference type="HOGENOM" id="CLU_060119_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWV9"/>
    <dbReference type="OMA" id="AKEYWCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010497">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR026698">
        <property type="entry name" value="UPF_C3orf38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21084">
        <property type="entry name" value="DENSE INCISORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21084:SF1">
        <property type="entry name" value="DENSE INCISORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15008">
        <property type="entry name" value="DUF4518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="33173" checksum="FDB475ABBEB9C1F9" modified="2014-04-16" version="1">KEMSGLSEKEKEGCSKILKSLSEADLLALNDTVTNRRIAVENIEEAQQAILIYSQNAEELLKRKKVQRDVIFSYLVKEGVVVSPAAEKHLLIKRTLEYWRSDNATAEASRVRVEDEATSSKAEQMTAGDYQVLAKQFCQWFFQLLNSQNPTLGQTSSDWGPQHFWEDARLKFCYSTGEQQEEEYQGAQLTSLRLLALAREEQLLFSPNLEPQGLKWVASPHGLVVVAVAGTIHRHRSCLGIFEQVFGLIRAPMDNSWKIKFVNLKILGKDGLEGGGQAMLPTVTYEHSDLQLLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXI6</accession>
    <name>W5MXI6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012738">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXI6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013123.1">
        <property type="protein sequence ID" value="ENSLOCP00000013095.1"/>
        <property type="gene ID" value="ENSLOCG00000010691.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SXGQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000284718"/>
    <dbReference type="HOGENOM" id="CLU_117689_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXI6"/>
    <dbReference type="OMA" id="PCNTEAH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010691">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF151">
        <property type="entry name" value="ICOS LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="124" mass="14247" checksum="F55E7467182264C8" modified="2014-04-16" version="1">VLYLCCCFKTFSSIFTVFAEVKTVTGNRGGDAVLPCNTEAHEDSRATVFWRYKTNIIVHSVNDGVENLSQQDSRFKNRTRMFPNEFRQGKFSLELSRLQDNDTGSYTCYIPSKSFQEHIELNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXT3</accession>
    <name>W5MXT3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Amyloid-beta A4 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 16">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012720">
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    <keyword evidence="10 16" id="KW-0034">Amyloid</keyword>
    <keyword evidence="16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
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    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="290"/>
            <end position="340"/>
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        <location>
            <begin position="354"/>
            <end position="545"/>
        </location>
    </feature>
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        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="192"/>
            <end position="268"/>
        </location>
    </feature>
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        <location>
            <begin position="379"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="231"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00016844"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00053261"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="746" mass="83611" checksum="53196886B8BA599B" modified="2014-04-16" version="1">SGVHLIARLMGLFLSPSPSQVPTDGSVGLLAEPQVAMFCGKLNMHINVQTGKWEPDPSGTKSCIGTKEGILQYCQEVYPELQITNVVEANQPVSIQNWCKKGRKQCRSHLHIVVPYRCLVGEFVSDALLVPDKCKFLHQERMDMCESHLHWHTVAKESCGDRSMNLHDYGMLLPCGIDRFRGVEFVCCPSEAERDDSAQLEEDDSDVWWGGADADYTDNSEPQEAGPGDREEAETALGEDDEDVNDEENVDDDEDDIEEPEASERTTNVAMTTTTTTTTESVEEVVREVCSEEAETGPCRAMLPRWYFDSSEGRCAQFIYGGCGGNRNNFESEDYCLSVCGSVLPTTAPSPPDAVDRYLETPGDENEHAHFQKAKESLEAKHRERMSQVMREWEEAERQAKNLPRADKKAVIQHFQEKVESLEQEAASERQQLVETHMARVEALLNDRRRLALENYLTALQANPPKPRHVFSLLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRPQVLTHLRVIEERMNQSLGLLYKVPGIAEDIQEQVDDLVQKEQVEVAEQLSSLQSEGRVSYGNDALMPSLSISTSPLDLLPPEEGGIGREGLGYIHPAGFGPANTQNQVEPVDVRPAPADRGLPTRPAGVKIGEVPEMRIETEERHSTGYEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLRKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY49</accession>
    <name>W5MY49_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-homologous end-joining factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034469">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY49"/>
    <dbReference type="FunCoup" id="W5MY49">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013308"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013336.1">
        <property type="protein sequence ID" value="ENSLOCP00000013308.1"/>
        <property type="gene ID" value="ENSLOCG00000010861.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698845"/>
    <dbReference type="KEGG" id="loc:102698845"/>
    <dbReference type="CTD" id="79840"/>
    <dbReference type="eggNOG" id="ENOG502QWAX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009940"/>
    <dbReference type="HOGENOM" id="CLU_076115_0_0_1"/>
    <dbReference type="InParanoid" id="W5MY49"/>
    <dbReference type="OMA" id="LPFYWHF"/>
    <dbReference type="OrthoDB" id="2155935at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010861">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22285">
        <property type="entry name" value="HD_XLF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.450:FF:000003">
        <property type="entry name" value="Non-homologous end-joining factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.210.10:FF:000001">
        <property type="entry name" value="Non-homologous end-joining factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.450">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053829">
        <property type="entry name" value="XLF-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21928">
        <property type="entry name" value="XLF_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLF-like N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="XLF-like coiled-coil region" evidence="10">
        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="35340" checksum="AF13046A168E17DB" modified="2014-04-16" version="1">MEVSGHSLEDLSYQPWVPVEIASTCFLAKVHFGETSYQLLLSDLDLVWHEQLSTEAIRQRAQELNRRLTAPVSSVFRHLRKVAQPRLEGAEHGASEAVFSHERVQDNLHVRVKSELAGVPFYWEFRCTPVPVTVVCEQLVRPLLAVSRLLHCQVQELTALLQRKDAEIQDYRESGVSLSRGRLETEVFVEQHYVEHFLSERLSEVSTMQNCYGFNQDIQKLCTAVAACGAGHKRNRSDSHSQNSPTNSGETLHHDEKCSAAENAQDKAEKVASSGVTDGFQQEQTVVLGSVSGTEKSAGSQRTSVKPKKKKAKGLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY73</accession>
    <name>W5MY73_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01039772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY73"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013360.1">
        <property type="protein sequence ID" value="ENSLOCP00000013332.1"/>
        <property type="gene ID" value="ENSLOCG00000010880.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW4X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5MY73"/>
    <dbReference type="OMA" id="TIARVYI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF966">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="53"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="328" mass="37467" checksum="3B6042461CCFFBEA" modified="2014-04-16" version="1">MKNDNDTHFLAAMEINTTFSTPVGFLISGFQNLQHSDYYFIFLAFVYLGTVVANFFLMYIIWVSECFHTPKYIAVFNLSVVDVSISTALIPKCIHQFLFESKFVSYETCLTQMLFVHFLHILESFSLVVMGYERLITICFPLRSSTINTNSRMIFIIGFCWVLSLISFVVAVVFITRLSFCQPVPVIKSYFCDHGPVFKEACSSYTSNWTIARVYIVSYFFVPLALISLSYVCIIAALSRISSAQGRWKAFKTCMAHLALVAMYFIPILVTYMIGWSNVLVDTNTRILNTSLSMTLPPLLNPIIYTLKTEEVMGQIKKFIRKRKASPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ67</accession>
    <name>W5MZ67_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Small ribosomal subunit protein mS27</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">28S ribosomal protein S27, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial ribosomal protein S27</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the mitochondrion-specific ribosomal protein mS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017137">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ67"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013676"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013705.1">
        <property type="protein sequence ID" value="ENSLOCP00000013676.1"/>
        <property type="gene ID" value="ENSLOCG00000011134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696822"/>
    <dbReference type="KEGG" id="loc:102696822"/>
    <dbReference type="CTD" id="23107"/>
    <dbReference type="eggNOG" id="KOG4570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007246"/>
    <dbReference type="HOGENOM" id="CLU_065381_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ67"/>
    <dbReference type="OMA" id="REDIDHA"/>
    <dbReference type="OrthoDB" id="19830at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011134">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000232">
        <property type="entry name" value="28S ribosomal protein S27, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034913">
        <property type="entry name" value="mS27/PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019266">
        <property type="entry name" value="Ribosomal_mS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10037">
        <property type="entry name" value="MRP-S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="372"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="414" mass="47922" checksum="E574BA61F689F55B" modified="2014-04-16" version="1">MAASMLQRCVLSTRCLTKYATQSLTARRYLLSPAYTDTAKWEGRRKDPQNLAELAFLMDKTYERKLPVSSLTIARFVDNISSREEIDHAEYYLYKFRHSPNCWYLREWTTHSWVRQCLKYEARDKALHTLKNKVQYGIFPDDFTFNLLMDSFLKDKDYEGAFSVVVEVMLQEAFDLPSTQILSLHALTKYLETKPEFKWEQERNLGAAFLIAAMKQENTVGFSSQLLGYSLLGKVELCKGIHAVFHQMPLMWTSGYLSRALEVMEKVSLSHGDIRLSKEVIEFLEVILLDCSSVVEATTTEGTDDSQETKTKAADLDDEDLLEREKLAEYTNTFKELCSRLQSLDKVDSRSMVTLTTHLVDEQLASVEGADIALYEKKLQEWEEEREQLIRREREMREKARLEREARLAAKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZB4</accession>
    <name>W5MZB4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">HAUS augmin-like complex, subunit 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017142">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627168.1">
        <property type="nucleotide sequence ID" value="XM_006627105.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZB4"/>
    <dbReference type="FunCoup" id="W5MZB4">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013723"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013752.1">
        <property type="protein sequence ID" value="ENSLOCP00000013723.1"/>
        <property type="gene ID" value="ENSLOCG00000011169.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697343"/>
    <dbReference type="KEGG" id="loc:102697343"/>
    <dbReference type="CTD" id="115106"/>
    <dbReference type="eggNOG" id="ENOG502QSQA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006029"/>
    <dbReference type="HOGENOM" id="CLU_063322_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZB4"/>
    <dbReference type="OMA" id="CEAQMES"/>
    <dbReference type="OrthoDB" id="5372507at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011169">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02087">
        <property type="entry name" value="HAUSAUGMINL1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="116"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="286" mass="32032" checksum="A07F9339651D8EA9" modified="2014-04-16" version="1">MCEKTTKVTQWLNKIFGDQPVPYYEVNSRTIDVLYQLAQSSEARCKDETLVLEDLKQQAAEYSADGSYVQDLLLQAVGLSSSSLSKRAFSNLSVLEGSAVALNTKDTSLASFVAAMNDLTAELMTVEKKNRQMDQDLTILRRKLTAAIVQRKALQEDLTKTEESEEVENARAENRLQNLDFLRAKSEDLSFRIKTAEDQLSATGMEPSLTHQAIVELSEKLAKLKQETVPLKKKLESYLDLTPNPSLAQVKIEEAKRELAAIDAELTMKVDIVNIALPEQRGRHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZH3</accession>
    <name>W5MZH3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L37</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017145">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013782.1"/>
        <property type="gene ID" value="ENSLOCG00000011220.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000005254"/>
    <dbReference type="HOGENOM" id="CLU_150908_0_0_1"/>
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        <property type="term" value="F:rRNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn-binding ribosomal proteins"/>
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    </dbReference>
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    <keyword evidence="3 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="78"/>
            <end position="97"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022730"/>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="97" mass="11041" checksum="F565A11E86C513E9" modified="2014-04-16" version="1">MTKGTSSFGKRRNKTHTLCRRCGSKAYHLQKSTCGKCGYPAKRKRKYNWSAKAKRRNTTGTGRMRHLKVVYRRFRNGFREGTTPKPKRAAVAASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N042</accession>
    <name>W5N042_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Regulator of G protein signaling 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006630729.2"/>
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        <property type="protein sequence ID" value="ENSLOCP00000014001.1"/>
        <property type="gene ID" value="ENSLOCG00000011392.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685187"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RGS"/>
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    </dbReference>
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        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING LOCO"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00615">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="81"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="218" mass="25392" checksum="9023761AC8DB0AAC" modified="2014-04-16" version="1">MRQRKLLLPGREKGRGFQKATSEFEKVAGETQRCPCCQRRMFNRAVNRLSRKRPPSEIQDINVFASTSQQCVTGAVKWSISLENLLEDPEGVRKFREFLKKEFSEENVLFWLACEEFKKIQDMQKLRDKAKYIYATYLSSTSTTQVNVEGQSKLNESMLDQAHPLMFEKLQEQIFNLMKFDSYKRFLRSDIFLKENGQKVSGGSEETVAKRASIVYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N313</accession>
    <name>W5N313_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase assembly protein COX20, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5N313"/>
    <dbReference type="FunCoup" id="W5N313">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015022"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015051.1">
        <property type="protein sequence ID" value="ENSLOCP00000015022.1"/>
        <property type="gene ID" value="ENSLOCG00000012213.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3BD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016158"/>
    <dbReference type="HOGENOM" id="CLU_101495_1_1_1"/>
    <dbReference type="InParanoid" id="W5N313"/>
    <dbReference type="OMA" id="IPYAWDS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012213">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022533">
        <property type="entry name" value="Cox20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586">
        <property type="entry name" value="CYTOCHROME C OXIDASE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12597">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02049">
        <property type="entry name" value="PROTEINF36A"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="120" mass="13463" checksum="867EFB2B5226EEE0" modified="2025-10-08" version="2">MAIPNDSHTVPSLSRQSFKLLGILDIQNTPCAREAVLHGSVGSLFSGLSHFLATSRVRRSFDIGVAGFMLITLGSWFYCRYSNAKLRVQQRLIQDGMKNKVMYEGTSLDPTRKQNGEDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3H7</accession>
    <name>W5N3H7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="24">ADAM metallopeptidase with thrombospondin type 1 motif 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with COMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032675">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N3H7">
        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="MEROPS" id="M12.237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015215.1">
        <property type="protein sequence ID" value="ENSLOCP00000015186.1"/>
        <property type="gene ID" value="ENSLOCG00000012331.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691066"/>
    <dbReference type="KEGG" id="loc:102691066"/>
    <dbReference type="CTD" id="81792"/>
    <dbReference type="eggNOG" id="KOG3538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155855"/>
    <dbReference type="InParanoid" id="W5N3H7"/>
    <dbReference type="OMA" id="YGLHHPV"/>
    <dbReference type="OrthoDB" id="412680at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012331">
        <property type="expression patterns" value="Expressed in larva and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000004">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF189">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 18 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10 17 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867201" evidence="21">
        <location>
            <begin position="25"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="22">
        <location>
            <begin position="254"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="23">
        <location>
            <begin position="1529"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1026"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1103"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1156"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1026"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1106"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1156"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="active site" evidence="16 19">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="330"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="359"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18 19">
        <location>
            <begin position="378"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="503"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="561"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="565"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1567" mass="176295" checksum="123A20FED2AFB078" modified="2014-04-16" version="1">MLCLQESRILFLLQFVFFLAVSHARSPQLGNFYFPDKEQEQFLRSQTEFALVHPQKVGRDGEFVSHNLAHHIKLRSKRDVEVAEHRVFYKLNHNGQDIIFDLTINHHLLSREYFLEWRSGSPNATKMHSSTLNSCHFIGTVENSSSVAGVAAISTCNGLRGFFSLPEGSYFIEPVREHEPEEGEQQPHVIYQSSFPQQPQRQRRSAELHHGRMPCGLKDSYLDSLRTERRRENWERKHSKEGRQIAQRSVSKERWVETLVVADPKMIEYHGIENVESYVFTIMNMVTGIFHDASIGNAIHIVLVRLILLQEEEKGLKIVHHADNTLASFCTWQKSINPQSDTHPAHHDVAVLITRKDICAGMNKPCETLGLSHLSGMCQAHRSCNINEDSGLPVAFTVAHELGHSFGIHHDGQGNDCEQGRHPFIMSRQLQYDASPLTWSTCSKDYITRFLDRGWGSCLDDRPSKKDLTAPAAAPGVLYTIHQQCQLQYGPSATFCEEVENVCQTLWCSVNGSCRSKLDSPADGTRCGEDKWCISGECVIVGKLPETVNGGWGVWSHWSHCTRTCGAGVQSAERECNNPKPEYGGKYCTGERKRYRICNTKACPKDRPTFREMQCSEFDTVPYKNELYEWIPVANLVNPCELNCRPVDEQFSEKMLDAVTDGTPCFMNNSSRNICINGVCKAVGCDYAIDSNAVEDRCGICLGDGSSCHTVRKTFVAKEGSGYVDVGMIPEGARDIVVKEVEEAGNFLVLRSEDPEKYYLNGNFIIQWNGEYLAGGTTFYYERKGNLENLTSPGPTREPVWLQLLFQEQNPGIEYEYTIRNSRVKGNDISVLEYKWKYGAWTDCSATCGTGFQRQPVRCFETETGVVEETLCDPDTRPLDRQRKCKTMDCPARWWVGSWQLCSATCGPSGVKKRTVLCVRTVGEEERVLPFTECKHLLKPKSLVPCNREVLCGSEWAVGNWSKCSVSCGGGVRSRSVSCVAVATEKCDPSKKPHSKLLCSLQSCPSSRRTFPPSFLKFRKIFTKSSPNNGTLKPSSTTLQPDPMTTTPTSTWTTTAMTITSPSVVTSVLSLSTWANPIAEDTVDSEDYYFNFIVVKNGSTKGIPKISRDSITNRKDPEPNNGLDEDHGSTPDIIFADKHVRYTPGYDYVTDDSTLTRKEANEGSPHKGINSSRDLYRTTAPTPYTSQSPLARTTLPYHRTVSTGKTHIKPSNKWLTRTRTATMSPIRYKTKLTTPTTSWMPHSETEKIHTAVTVYPTIKVLKEHFKKVSDPWATRNATAAPEVYGSRDGHFLSFTGNYENIHGKPHISRNAHWVIGNWSECSTTCGLGAVWRTVVCSTNKDSDCENIKRPDPARRCHLRPCAAWKSGEWSKCSEGCTEATKVRDVQCIDTQSKRQLRPFHCQALAFKPSVSAPCNHQRCLEWRLSPWSKCSKSCGPGLRERLVYCPEYNRCNLKERPSSTEPCSERLCTKWVADAWEECTVTCGGGVQHRVVNCVNEETKNVENNSLCEKTTRPDSFQKCHLQECEKSKELSCSKNHMSTRFCEKLKLLGRCSVSSIRKQCCSTCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N463</accession>
    <name>W5N463_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217373.1">
        <property type="nucleotide sequence ID" value="XM_015361887.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217374.1">
        <property type="nucleotide sequence ID" value="XM_015361888.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N463"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015422"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015451.1">
        <property type="protein sequence ID" value="ENSLOCP00000015422.1"/>
        <property type="gene ID" value="ENSLOCG00000012514.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697118"/>
    <dbReference type="CTD" id="6774"/>
    <dbReference type="eggNOG" id="KOG3667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244905"/>
    <dbReference type="HOGENOM" id="CLU_014189_3_0_1"/>
    <dbReference type="InParanoid" id="W5N463"/>
    <dbReference type="OMA" id="DCANSPM"/>
    <dbReference type="OrthoDB" id="19300at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012514">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003401">
        <property type="term" value="P:axis elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061026">
        <property type="term" value="P:cardiac muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070365">
        <property type="term" value="P:hepatocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060119">
        <property type="term" value="P:inner ear receptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033210">
        <property type="term" value="P:leptin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097421">
        <property type="term" value="P:liver regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048570">
        <property type="term" value="P:notochord morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060252">
        <property type="term" value="P:positive regulation of glial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035677">
        <property type="term" value="P:posterior lateral line neuromast hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903010">
        <property type="term" value="P:regulation of bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043434">
        <property type="term" value="P:response to peptide hormone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16853">
        <property type="entry name" value="STAT3_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16847">
        <property type="entry name" value="STAT3_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000012">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.532.10:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.20:FF:000003">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000003">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000012">
        <property type="entry name" value="Signal transducer and activator of transcription 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="581"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="789" mass="90228" checksum="44CF59F5F97C597D" modified="2014-04-16" version="1">MAQWNQLQQLDPRYLEQLYHLYSDSFPMELRQFLAPWIESQDWAYAANKESHATLVFHNLLGEIDQQYSRFLQENNVLYQHNLRRIKQHLQSKYLEKPMEIARIVARCLWEENRLLQTATTTAQDGQASHPTGTVVTEKQQILEHNLQDVRKRVQDMEQKMKMLENLQDDFDFNYKTLKSQGEISQDLNGNSQAAATRQKMAQLEQMLTALDQLRRQIVTEMAGLLSAMDFVQKNLTDEELADWKRRQQIACIGGPPNICLDRLETWITSLAESQLQIRQQIKKLEELQQKVSYKGDPIVQHRPALEEKIVDIFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKESGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANSDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNHPKNVNFFTKPPVATWDQVAEVLSWQFSSTTKRGLTIEQLTTLAEKLLGPCVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGITFTWVEKDISGKTQIQSVEPYTKQQLNSMSFAEIIMGYKIMDATNILVSPLVYLFPEIPKEEAFGKYCRTENTEQPDFTDPGSTVPYLKTKFICVTPSPSVFMDMPDSELLANGYSGTNSGNTSDLFPMSPRTLDSLMHNEVQEPNPGGGPLESLTLDMEFTSEAASPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4B2</accession>
    <name>W5N4B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DMRT like family A1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4B2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015471"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015500.1">
        <property type="protein sequence ID" value="ENSLOCP00000015471.1"/>
        <property type="gene ID" value="ENSLOCG00000012567.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688647"/>
    <dbReference type="KEGG" id="loc:102688647"/>
    <dbReference type="eggNOG" id="KOG3815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160640"/>
    <dbReference type="HOGENOM" id="CLU_038477_1_0_1"/>
    <dbReference type="InParanoid" id="W5N4B2"/>
    <dbReference type="OMA" id="RELQFMY"/>
    <dbReference type="OrthoDB" id="9942608at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012567">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14417">
        <property type="entry name" value="CUE_DMA_DMRTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005173">
        <property type="entry name" value="DMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046472">
        <property type="entry name" value="DMRT5_1_DMB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF71">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03474">
        <property type="entry name" value="DMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20624">
        <property type="entry name" value="DMRT5_DMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="8">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="6">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="451" mass="48474" checksum="C1FB4E49CC3A1C98" modified="2014-04-16" version="1">MEGSSRPLPQSGLPGHPPSSISVGGLQVSASLLRPPPLFLRAAAAAACNPSLDRGYPRTPKCARCRNHGVVSALKGHKRFCRWRDCVCAKCTLIAERQRVMAAQVALRRQQAQEESEARELQFMYTGAGTSEPGLGVMASVGAPGGTGQLSTPSARTPGYEPFGIEDHKDEDKISTYNVYNGFLARPLFAPHSAQLVSSTLRKNSACPNLRENSHSSLEKSEKDKGVQSPGSDQLSEHAESPRSLSSSDLESGSESERHKDFSSPEPSAPASSSRHRDPTDIMMKIFPHHKRDTLEAVVKTCKGDIVKAIELVLNSKEDKPLSGDSDIPTPESSTFHRPSGFGITGGGFGQLSSKSAFSPLQTTPSSAGDSIYGLNPRLGISPLRLAYSSSGGAITSFMSPYVTPGLMPAFPLRPPMDYSFPGMIRDFSYLQNKEPLCNSGLYSRLSHEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4J2</accession>
    <name>W5N4J2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSTCD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630074.1">
        <property type="nucleotide sequence ID" value="XM_006630011.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015201259.1">
        <property type="nucleotide sequence ID" value="XM_015345773.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047439.1">
        <property type="nucleotide sequence ID" value="XM_069191338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4J2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015551"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015580.1">
        <property type="protein sequence ID" value="ENSLOCP00000015551.1"/>
        <property type="gene ID" value="ENSLOCG00000012628.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690468"/>
    <dbReference type="KEGG" id="loc:102690468"/>
    <dbReference type="CTD" id="79807"/>
    <dbReference type="eggNOG" id="ENOG502QUFE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004446"/>
    <dbReference type="HOGENOM" id="CLU_013673_1_0_1"/>
    <dbReference type="InParanoid" id="W5N4J2"/>
    <dbReference type="OMA" id="WTRFCEV"/>
    <dbReference type="OrthoDB" id="206598at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012628">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000125">
        <property type="entry name" value="Glutathione S-transferase C-terminal domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13369">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13369:SF0">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="6">
        <location>
            <begin position="409"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="624" mass="69013" checksum="90550004316E7D94" modified="2014-04-16" version="1">MKHTNSKRGEECLYLDISDQSEEAILPLHTSITLFLLSYCDSKLFRVFLVPSRPDPAQSWRHRVPGGLAASVVSEQDLPALVRSCCLPAVLEEEGRYCRAGLAVVLRHIIQRTCEEDPSRKDVAALLGFKKTCLKACAEVSQWTRLCELNIPSAVEEFLRQPAGPDPEIPAAILRLEKKLEEPVKVHNDDKIRRQKLQQQKEEAGSATQPASFRESVNSEELELRAALAKLSVEDTPLSATRGPSNIRRVKTTELPALEHVFAEGLYFTLTDLVLLPCLHHFLVSLHHRESNKLARLPLLQRWYRRVQEVPGVARAAGTCGIGFLLLPDSGWHPALESRELCPPEQEPAEKAQAPFIGGPRPTMTKLRECGIEAVFTPHPCPSWTLDWNSLPTAVNPTEGKMSDVRALRKQQQLNNLVAMVTKAAQPGDTVVDFCSGGGHVGIVLAHTLPECQVLLIENKEESLVRAKNRCRELGLNNIGFIQANLDYFTGPFQVGVALHACGVATDMVIELCVKAQAAFVISPCCYGFIQNTLKFTFPRSKQFLDALSYKEHMILCRFADQTAVQLPAGRRLIGKQCMGLVDLDRSWAAESHGYCVGVMTMEPGSCSPKNNMLVGVPDGRSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5L5</accession>
    <name>W5N5L5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5L5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015924"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015954.1">
        <property type="protein sequence ID" value="ENSLOCP00000015924.1"/>
        <property type="gene ID" value="ENSLOCG00000012931.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU0D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018900"/>
    <dbReference type="HOGENOM" id="CLU_287249_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5L5"/>
    <dbReference type="OMA" id="VCYESHL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012931">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032040">
        <property type="entry name" value="ELYS-bb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052620">
        <property type="entry name" value="ELYS/MEL-28_NucAsmblyFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025151">
        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583">
        <property type="entry name" value="ELYS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583:SF8">
        <property type="entry name" value="PROTEIN ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16687">
        <property type="entry name" value="ELYS-bb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELYS beta-propeller" evidence="4">
        <location>
            <begin position="26"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ELYS-like" evidence="3">
        <location>
            <begin position="721"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="910" mass="104661" checksum="4CF2741F9FCB2767" modified="2014-04-16" version="1">MLPSSKLNYSIPFSEPAKHWCTGDQSQVKGAFSKEFPGICWVVCGSNLTVIRVLTGECLSSHDFTDKTVVQVTEFTWRKNWYLLVELLDSNGGSLCCYCLSSSRVLRAISIPRKRDSILLIDESLLLGCKDEEYLHSVLKCFSGIAVIGTKQGEILLLDLALKDPTVFSTEKKMSECEEVLRCEGKIEDNLDVSSLKQKHPCLYLHSHQGAPATSLLFIKPTNQVVAGNSTGKLQFWNLQYLNKECEIQLESGLIPLVSLQFQESFPFHVPRCYLWAVSSESSSCWGKSSIRVNLVKLLFRDFNQSSQVRYQGFLHGNVCHTQKLCDGADLNPCVSWCRLLSCRIISGYDRSVQRNLDYGNLAIFAWEIKKPELLTFVAVFSLQQWLQKEMPEPLKSNNELEGCPYLNLWLLAPRSSHPLPILDTTVFKDLHCSKWKNYLPLICKEINRKYLSSLYCTDVMQMLVIACVFIAKNKHKKKTIAILTEFEKKEKETSIYSYSKLILKGQNTCQFCSTLRSDFSEEDTLQCLLTRALTNHRIRRIICWMKNLSNEADPDLDNTLCVLSSWVQKMVDHGKKQFLALCKYDSHWMYMDHQSLSHVLHLLQFLKNLLTIIQFMRKLPHLVQCPVVNAVHWDQEYRTTNQYIFLVRLVHWLGLTGLLPKPTANIPMTRISTRTCSSYSILVIFYTVLKVIIGKTFRNKEEEDGLLIHGIVSHLNKIYGPIWSKEEQHSELYPPPNLLAVLKLFLLPCIDTVSLQSTFLYFLLDITHFLRCEDNVLTSFTHTFDVPLGFCQQIKGLWFLDHGHISTSLDLLLHPSTMRPWHSWQHSLIIQTLLKEGESLKALHHIHQIQPPIEKPKDLKLYIDVFLHNRCIAEAWALLREIEDPGDDIFKHFLQNCEDLGLYLDLSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6F8</accession>
    <name>W5N6F8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031899">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6F8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016217"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016247.1">
        <property type="protein sequence ID" value="ENSLOCP00000016217.1"/>
        <property type="gene ID" value="ENSLOCG00000013163.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXEZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154902"/>
    <dbReference type="HOGENOM" id="CLU_293662_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6F8"/>
    <dbReference type="OMA" id="PSIEMKP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013163">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032287">
        <property type="term" value="P:peripheral nervous system myelin maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052082">
        <property type="entry name" value="Myelin_sheath_structural"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348:SF16">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348">
        <property type="entry name" value="PERIAXIN/AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="268"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="515"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1172"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1192"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1206" mass="132047" checksum="2EF9F04C6E7E3E91" modified="2014-04-16" version="1">ESFHVSVIGTFYSISHPSTGSGRQLKGPEPDNEYDSVDETLEFLEEERPRPQGSSPVYECPSRNRSFRAGQTGCEGFHPTNDQRPSAFQSNEVHARLFDRQSETADESELIVKTQTEACAKGLIVSGGGKEGIFVKDVLHESPASTIPCMREGDQILSATVFFDDIRYEDALQILEHAKPYKVQFCLKRKSPATSAIETMFQHEKNSELAKSLNISVKCSPIQCASKYSKGETGTTVERGRTKTPRRQEARISWPKFPSFTRERKMYFKRSHSTSEAEEQKTLDMSPTTSDTESPKPGEEQIKNKRTPEGKRRKKKLRRQQVEMSKKGIQPRSSETEMQTPKFDSGDVYASDKDSPFLKGDVHMSKPQTETKDTELEQPTQGKKTELYKPKIPKSVISLPNVKTTEREDDLNKSDKTTTEAEASILQPVTGIKITETKSELDLSQSIKADLDVKSPEISTTANIKMPKPQLEAEGQDKDVDIEGSHGKFKMPSFKMPKFGFSRKEGVDIDAPIDDIKAPNADIEKPDSQSQLSKMPDLDISLPKPDLEVKGPEVEFKAPALEGKVDVPEIEAKGGRGKFQLPHMKLPSFGISTPDIKVPKIKGDTTLPTVDIPLPKADLDVKSPELTVSGEKPAVDLMGPSMDIEGKGTETEGEGSKFKLPKFGISLPKVKGTGLDISSPKPEVDITLPEAKIQAKVPEVDVEPPSVEGKIEVPEIDGKGLDIKLTKPKISLPKSGISKADMKAPEAEIDANLPKGDVSLPKRAVEIKRPEVEIKGPRIVAEVKDQDIDIEGSHGKFKMPSFKMPTFGFSTPKAQAEAPEVSADISIPDVDVPTGKVGVDIEAPRVDVEKPDRKFQMPKVKMPDMDISLPRVKGPEIDIQLPKAQAGVSVSKPDLDVKGPEVELKAPALEGKVDVPEIETKGGRGKFQLPHMKIEGPDLDVDLKGKVPEIEGEGSKFKLPKFGISLPKVKGTGLDINAPKPDVDIRLPEAKIQAKVPEVDIEAPSVEGKIEIPEIDGKGLDVKLKKPKKSLPKFGISKADMKVPEAEIDVNLPKGDISLPEGAVEIKGPEVDIKGPKIEAEVKDKDIDIEGSHGKFKMPSFKMPKFGFSTPKVKAEAPEVSTDISIPDIDAPSGKVGVDIEAPRVDVEKPDSKFQMPKVKMLDMDISLPRVKGPEIDIQQPKAQADVSISKPDLDVKGPEVDLKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6H4</accession>
    <name>W5N6H4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA selenocysteine-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the early steps of selenocysteine biosynthesis and tRNA(Sec) charging to the later steps resulting in the cotranslational incorporation of selenocysteine into selenoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM TRSPAP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002341">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6H4"/>
    <dbReference type="FunCoup" id="W5N6H4">
        <property type="interactions" value="1515"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016233"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016263.1">
        <property type="protein sequence ID" value="ENSLOCP00000016233.1"/>
        <property type="gene ID" value="ENSLOCG00000013175.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166060"/>
    <dbReference type="HOGENOM" id="CLU_016304_3_0_1"/>
    <dbReference type="InParanoid" id="W5N6H4"/>
    <dbReference type="OMA" id="RNEREYN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013175">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12612">
        <property type="entry name" value="RRM2_SECp43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000159">
        <property type="entry name" value="tRNA selenocysteine 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000166">
        <property type="entry name" value="Trna selenocysteine 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034510">
        <property type="entry name" value="SECp43_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040434">
        <property type="entry name" value="TSAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041085">
        <property type="entry name" value="TSAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37457:SF1">
        <property type="entry name" value="SIMILAR TO HUMAN CHROMOSOME 6 OPEN READING FRAME 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37457">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17654">
        <property type="entry name" value="Trnau1ap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="35139" checksum="A1B46A568A61C004" modified="2014-04-16" version="1">MFNRMASLWMGDLDPYMDENFIKQAFSTMGETAYGVKIICHRVTGGSAGYCFVELADEASVDRCVLELNINFQPMHYYTTQVFVHYTFYGTLSQAVIPEYSVFVGDLTPEIDDFQLYEFFVKKYPSCKGGKVVTDQFGNSRGYGFVKFSDEKEQRKAMEECQNATGLGGKPIRISIAVAKSNKMNYQHNQNYYTQYYQQYQNYYSQWGYDPYGSYNYGYGPYGPAPSAPGMATPLASTDGTTVGTDAQASAEKSAEDAEEEPVEDPALHMDIDTLNRQFMERSEELYDSLMNCHWQPLDSVTSEIPAVANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6L7</accession>
    <name>W5N6L7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fibrillin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016276"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016306.1">
        <property type="protein sequence ID" value="ENSLOCP00000016276.1"/>
        <property type="gene ID" value="ENSLOCG00000013197.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183158"/>
    <dbReference type="InParanoid" id="W5N6L7"/>
    <dbReference type="OMA" id="DPKCHAG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013197">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000023">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000058">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000071">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000086">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000087">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000097">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000159">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000007">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000009">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000011">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000133">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000003">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000008">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000244">
        <property type="entry name" value="Fibrillin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000020">
        <property type="entry name" value="Fibrillin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000096">
        <property type="entry name" value="Putative fibrillin 2"/>
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        </source>
    </evidence>
    <sequence length="2869" mass="311053" checksum="CAC2CA223A32B782" modified="2014-04-16" version="1">MWPGWLLKVSLGLAAVLSLSVSSAEGAAQDSEADKYSEPKANSRTKRRGGSQDVLKGPNVCGSRFNAYCCPGWKTLSGGNQCIVPICRSSCGDGFCSRPNMCTCPSGQIAPSCGSKSVQHCSIRCMNGGSCAEDQCMCQKGYAGAHCGQPVCENGCLNGGRCVAPNRCACTYGFTGAQCERDYRTGPCFTSISNQMCQGQLTGIVCTKTLCCATVGRAWGHPCEMCPAQPHPCRRGFIPNIRTGACQADVDECQAIPGLCQGGNCINTVGSFECKCPAGHRFNEATQKCEDIDECATLPGICNGGECSNTAGSYFCRCLQGYYASVDGSRCIDGRSGYCFASLVNGRCGNQLSQHMTKMQCCCDSGRCWSDGSAPEMCPMRGTDEYRKLCAMPFPPDQPIPRIPEIPPAVPGVHPGQPGVDVPVQPGVPIIPGIPGIPPVILPQNITNICLRYRNLCINGVCVPTPGSYRCECNTGYRLDVRGECIDDDECDRNPCVNGNCVNSQGSYFCQCSTGFQSTAGRTECRDVDECVANGRICNNGRCVNTEGSFHCVCNAGFEISSDGKNCQDQDECLIRNMCLNGMCINEDGSFKCICKPGFLLDPTGRFCTDIDECETQGMCMNGHCVNTEGSFRCDCLPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCVRPFFGAVTKSECCCANIEYAFGEPCQPCPPLNSAEFSALCSKGIGTTTNRHPSNINECALDPDTCQNGVCENMLRTYKCNCNLGFEVDSTGKNCIDIDECARSPHLCDNGQCRNTPGSFTCLCEKGYVFNPETDVCEDIDECESNPCVNGDCKNIKASFVCECPPGSVLDSSGTTCIETGTGNCWLTIINDRCEININGATLKSHCCATLGVAWGSPCTKCVPDTVCSKGFARVRGTTCEAIDVNECEVFPGVCINGKCVNTVGSFICQCPSGKTVDATGRTCIDLRTEFCYLTHEDERCVSPIPGKHRVDACCCSVGAAWGPECDECPEKGTPEYTQLCPRGPGFSHRGDFINGKPLLKDINECKMIHSLCTNGKCRNTFGSFRCKCDSGFALDSEERNCTDIDECRISPDLCGQGTCVNTQGDFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGECLNTEGSFRCVCPEGHEVASDGSACLDINECELSDKLCKNGRCVNMIGHYQCTCNTGYKSTEDKLSCVDIDECTIQNGQCETYCTNSEGSYECSCRTGYALMPDQRSCTDIDECEDNPDICDGGQCTNIPGEYQCLCYDGFMSSEDMKTCLDVDECELNPNICLSGNCENTKGSFICHCDMGYSVRKGTTGCTDINECEIGAHNCDRHATCTNTAGSFKCSCSPGWIGNGIKCKDLDECSNGTHMCSSHADCKNTMGSYRCICKEGFSGDGFFCTDSDECAENVNLCENGHCLNVPGGYRCECDMGFIPTLDGKACEDIDECSFPDICVNGRCRNIPGLFRCECHTGYELDRSGGNCTDINECADPTTCISGICVNTPGSYVCNCPPDFELNPTRVGCVDTRSGNCYLDVRARGDASLSLECSNEIGVGVSKASCCCSLGRAWGNPCEACPAINSSEYKTLCPGGEGFRPNPITVILEDINECQELPGLCQGGNCINTFGSFHCECPRGFELNEETRICEDVNECERPGICGPGQCYNTVGNYTCICPPDYMQVNGGNNCMDMRRSLCYRNFYSDNGTCDGELAFNMTKKMCCCSYNIGRAWNKPCEQCPIPSTNEFAILCGSERPGYIIDIISGKPIDIDECRDIPGVCENGVCINMIGSFRCECPMGFVYNDKLLICEDIDECQNGPVCQRNAECLNLPGSYRCECKPGYRFTPTGQCLDRNECAEIPNICSHGQCIDTVGSYRCICQNGFKPTADASMCIDVDECERQPCGNGTCKNTVGSYNCLCYPGFQLSHNNDCIDTDECSTQRGLCRNGNCINTIGSFQCVCRDGYELTADRRICIDINECVVNPGTCGPGICQNLDGSYRCICPPGYYLKDEKCEDIDECAQSPDICVFGTCSNTEGSFKCLCPSGFVLSSSGRRCIDERRHYCFSKFEDGRCQAPRPLNSTKSECCCSAMPGQGWGDPCDLCPGRNDTLFALLCAGVGLHVDKYDNRVETDLDECSADPNTCKNGQCINTDGSFRCECPLGFRLDFSGVNCEDTNECELGSPCGNGTCTNLIGAFECTCDEGFEPGPMMTCEDVNECAQNPLLCAFRCVNTVGSYECKCPKGYVLREDGRMCRDQDECEDGIDDCDTRGMTCKNLIGTYMCICAPGYVRQPNGDGCMDMNECSAKPGICENGRCVNTVGSYRCVCNEGFSPSSTLTECIDNRQGFCFTEVLQSLCQMTSTSRNPVTKSECCCDGGRGWGSSCELCPLPGTTQYKKMCPLGPGYTTDGKDIDECRVIGGLCRNGQCVNSIGSYSCICKPGYTTDISGTSCVDVDECVQSPKPCNFICKNTEGTYLCSCPRGYILQEDGKSCRDLDECSTKQHNCQFLCVNTIGGFTCKCPPGFTQHHTACIDNNECATDPGLCGAKGVCQNTPGSFSCECQRGYLLDQNGQNCEDVDECDGNHRCQYGCQNLIGGYRCSCPQGYLQHYQWNQCVDENECLNSQVCGGASCHNTLGSFRCICPTGFQYEQFSGGCQDINECVSPQNPCNYGCSNTDGGYTCGCPTGYYRAGQGHCVSGVGFGQGLPIGGDTDDNSLSPEACYECKINGYPKKGRRRRSTNGIGDEENEQEAMSLDSVDTEDTLAFSFNITDLSNKDHILEFVPALNTLTNHIRYVIDFGNDEGFFKINQKNGISYLRLTKKQLLPGAYFVQISSVPLYKKKELAHLEDKYDKDYLTGELGDTLKMKVQIILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N772</accession>
    <name>W5N772_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeats and calponin homology (CH) domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N772"/>
    <dbReference type="FunCoup" id="W5N772">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016481"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016511.1">
        <property type="protein sequence ID" value="ENSLOCP00000016481.1"/>
        <property type="gene ID" value="ENSLOCG00000013372.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166804"/>
    <dbReference type="HOGENOM" id="CLU_008231_2_0_1"/>
    <dbReference type="InParanoid" id="W5N772"/>
    <dbReference type="OMA" id="RAPKQET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013372">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21273">
        <property type="entry name" value="CH_LRCH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF64">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="558"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="435"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="479"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="704" mass="76839" checksum="9B32CEEAF793384E" modified="2014-04-16" version="1">MAAGDDSPLPASIAASRSVEKALEEAAASGALSLSNRKLKEFPRTARNYDLSDITHADLSKNRLSEVPEELCQFISLETLSLYHNCIRSIPPSIGNLQALTYLNISRNQLSSLPTAVCELPLRVLIASNNKLPCLPSTIQALGNLRQLDVSCNELQSLPPEIGLLESLRDLNLRRNHLTTLPEELSELPLVRLDVSCNRVTRVPVCYRHLRHLQTITLDNNPLQSPPAQICTKGKIHIFKYLNIEACSKTTPEEFDRALRPAGINSCLSDQELFPARQYGGLDSGFNSVDSGSKRWSGNESADDFSDLSLRMAEISREQRNLREERPVHSLGDRVNGEAEQEDFIDSSVTEEDEEEHRSLPSPHRTAPPQFTVSNTPLSPLPVFLRSSPGGDSAEPLGATPSSPSAEERRRPETLQIWQERERAHQQRERREISLLKSTAKTGSGSTSAAQTGSGPRCLSPPSGSSFDSGSSIICLRQRCQSTDQPSSSSAISAPARSTSRTDTPPMPNPAGSPGAVQKPNSFLFRAQSRTNVKPTGSVQCLVETGRSESKASLLRSPREEREGITQLRKTLESRLKITLPEDLGEALSDGAILCQLANHVRPRSVSIIHIPSPAVPKLSAPKCRRNVENFITACRKLGIPETEVCASSDVLCSNLPGVLRTVEALTRLGGCVSPHISLLCPGFLLFYSLAMGLLYLSYCQLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7G3</accession>
    <name>W5N7G3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7G3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016572"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016602.1">
        <property type="protein sequence ID" value="ENSLOCP00000016572.1"/>
        <property type="gene ID" value="ENSLOCG00000013441.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSZF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234567"/>
    <dbReference type="HOGENOM" id="CLU_1083914_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7G3"/>
    <dbReference type="OMA" id="LECHYRR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013441">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025708560" description="Zona pellucida sperm-binding protein 3" evidence="3">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="5">
        <location>
            <begin position="77"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="257" mass="27792" checksum="1721ED958FB63A71" modified="2014-04-16" version="1">MSVSQPFALGVVLSTLCVFVTHSQGFAAWLGDISRNRARTRVRPDIPGPISPRLSPPPAPAPDVQLRAQERPRIRVQCGEATVRVSVDRLYFGEPGPVSAASLRLGEDPRAPGSCTPEPTASVPGAALVITARLHECGSVSRVTPQALVYLNKLVYSPSPAGGVLERASPVLVPLECHYRRKHRVSSEAVRPTWLPFTSTRSAGGLLRFSLRTMNEDWGSPRLSSVFYLGETVRLEAAVSSQGHPPLRLFVDRCVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7W1</accession>
    <name>W5N7W1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complement C1q-like protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01030978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638431.1">
        <property type="nucleotide sequence ID" value="XM_006638368.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7W1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016720"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016750.1">
        <property type="protein sequence ID" value="ENSLOCP00000016720.1"/>
        <property type="gene ID" value="ENSLOCG00000013562.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693841"/>
    <dbReference type="KEGG" id="loc:102693841"/>
    <dbReference type="eggNOG" id="ENOG502QSKV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162478"/>
    <dbReference type="HOGENOM" id="CLU_001074_3_1_1"/>
    <dbReference type="InParanoid" id="W5N7W1"/>
    <dbReference type="OMA" id="VYHFSYH"/>
    <dbReference type="OrthoDB" id="6154955at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013562">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF33">
        <property type="entry name" value="COLLAGEN IV NC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867359" evidence="7">
        <location>
            <begin position="19"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="83"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="214" mass="22101" checksum="DA400947B7647507" modified="2014-04-16" version="1">MAPLQLLLLLSLLAASSASQQALGSCRIVCDPPASGAARGEHGVVVPLSAGAPGPPGPAGPPGKAGPRGSPGIPGPPGGPGTATRPRIAFYAALSELFTQQDVLRFTDVITNLGADYSPGSGKFTCRVPGVYVFSYHILKDGEHMWADLMLNNQVYASSTAQDASSKFDTAANSAVLQLKAGDEVYVKLDGGQVMKDGAQGRYSTFSGYLLYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N803</accession>
    <name>W5N803_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Monoacylglycerol O-acyltransferase 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030438">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N803"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016762"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016792.1">
        <property type="protein sequence ID" value="ENSLOCP00000016762.1"/>
        <property type="gene ID" value="ENSLOCG00000013592.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102692390"/>
    <dbReference type="eggNOG" id="KOG0831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234582"/>
    <dbReference type="HOGENOM" id="CLU_1558913_0_0_1"/>
    <dbReference type="InParanoid" id="W5N803"/>
    <dbReference type="OMA" id="DYLMSEW"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013592">
        <property type="expression patterns" value="Expressed in embryo and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF19">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="163" mass="18730" checksum="1731D6FC0C64A3BA" modified="2014-04-16" version="1">MEQEKTTWKEFIENISVLQWVLTFLIMGISCTILMLYLIFTSLWLLSVLYFIWMVIDWDTPERGGRRSDWIRNWSVWKHIRDYYPVTLVKTAELSPSKNFVLGYHPHGIMCAGAFSCFSTEGAGFSAAFPGIQSHLATLAGLFRLPLYRDYLMSSGTARLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8T2</accession>
    <name>W5N8T2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family F member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5N8T2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017041"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017072.1">
        <property type="protein sequence ID" value="ENSLOCP00000017041.1"/>
        <property type="gene ID" value="ENSLOCG00000013815.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155604"/>
    <dbReference type="HOGENOM" id="CLU_000604_36_6_1"/>
    <dbReference type="InParanoid" id="W5N8T2"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013815">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000688">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058770">
        <property type="entry name" value="PWI_ABCF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26051">
        <property type="entry name" value="PWI_ABCF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="182"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="484"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="701" mass="78918" checksum="83CBD74892905427" modified="2014-04-16" version="1">MATYVDILRSEFPEIDTEVFDYITGMTQSQSEWKGCPLYACPAVEFCLQPVTLFGEACADSQMLSHSLCHTFSYICNDCDSGQKQVLLDAPVQLSQMAGDCVTATNDLQGIWMIKRGQNTTVDAKKLEKAEAKLKAKNEKRTEKDSQKVASPLVLEEASASQASSKKDSRVDNSGKNKSYDIRIENFDVSFGERSLLQGAELSLAYGRRYGLVGRNGLGKTTLLKMLASRSLRVPPHISILHVEQEVAGDDTPALQSVLESDTEREGLLQQEREINARIASGSECGTLTDSRRRATSRGFVILAGLGFSPKMQQQTTKEFSGGWRMRLALARALFAKPDLLLLDEPTNMLDVRAILWLENYLQTWQSTILVVSHDRNFLNAVATDIIHLHSQRLETYRGDYENFIKTKEDRLRNQQREYEAQQQTATHVHVFIDRFRYNANRASQVQSKLKLLEKLPELKPIEKESEVTLRFPDNFEKLSPPVLQLDEVEFFYSSEQPLFSKLSVSADLESRICIVGENGAGKSTMLKLLMGELGPVSGIRQAHRNLKIGYFSQHHVDQLDLNVSSVELLMKKFPGRTEEEYRHQLGGYGISGELAVRPVASLSGGQKSRVAFAQMTMASPNFYILDEPTNHLDMETIEALAKALNKFKGGVVLVSHDERLIRMVCKELWVCENGGVQRIDGGFDEYRDILQEQFRREGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8Y4</accession>
    <name>W5N8Y4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mimecan</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Osteoglycin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8Y4"/>
    <dbReference type="FunCoup" id="W5N8Y4">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017093"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017123.1">
        <property type="protein sequence ID" value="ENSLOCP00000017093.1"/>
        <property type="gene ID" value="ENSLOCG00000013860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689260"/>
    <dbReference type="KEGG" id="loc:102689260"/>
    <dbReference type="CTD" id="541445"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157238"/>
    <dbReference type="HOGENOM" id="CLU_067583_1_0_1"/>
    <dbReference type="InParanoid" id="W5N8Y4"/>
    <dbReference type="OMA" id="CASCALC"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013860">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061975">
        <property type="term" value="P:articular cartilage development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043547">
        <property type="entry name" value="Mimecan/Epiphycan/Opticin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269">
        <property type="entry name" value="EPIPHYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269:SF1">
        <property type="entry name" value="MIMECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869427" description="Mimecan" evidence="15">
        <location>
            <begin position="25"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="296" mass="33253" checksum="CDF6C2073F5470F4" modified="2014-04-16" version="1">MSKLKPLIFSLILVLWVILAAVTAFTLDSEHHNNEKDIFRTPDDFLTALEAKKQETIIDYEDLPPADTEPAENEVPVLPTPKEAAELPTCLLCVCLSGSVYCEEVVPVITAVPALPKETAYLYARYNKIAKIAQKDFSEIPTLRRIDLTGNAISEIEEGAFSKLPQLEELSLAENRLVKLPTLPTKLTLFNANHNRLRTRGVKANAFRKLVNLSYLYLANNELEAVPPHLPESLRVLHLQNNNITAITDETFCKGNNTRYIRTNMDEIRLEGNPIILGKYPNSFTCLKSLPMGSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB07</accession>
    <name>W5NB07_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zgc:162200</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BicD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB07"/>
    <dbReference type="FunCoup" id="W5NB07">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017816"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017848.1">
        <property type="protein sequence ID" value="ENSLOCP00000017816.1"/>
        <property type="gene ID" value="ENSLOCG00000014473.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0999">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154471"/>
    <dbReference type="HOGENOM" id="CLU_014107_1_0_1"/>
    <dbReference type="InParanoid" id="W5NB07"/>
    <dbReference type="OMA" id="ARQDTFI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014473">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072393">
        <property type="term" value="P:microtubule anchoring at microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018477">
        <property type="entry name" value="BICD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233">
        <property type="entry name" value="BICAUDAL D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233:SF12">
        <property type="entry name" value="BICAUDAL D HOMOLOG 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09730">
        <property type="entry name" value="BicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="402"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="578"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="197"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="346"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="705"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="780" mass="86955" checksum="F0D4587147684C56" modified="2025-10-08" version="2">MSAGAMLQEGEAEAELQRLARELQEANEEKLQAARYGLAVLEESAALKQRYAELEGEHEALRQELQQLKEALADSVSSHKRAAADGESREETLLRETASREAALAERIEELQAELRQARVTLANAQAEGDRLAALSAQLKKTSCYRLFGEEDLGYVLFLPADSSVSELRAVELFCFCGDESSLLLQHAHEFAYWVNVYKLQVEFETVKLELKNKEEELELLRGQLEEAGRLREIAERQLDEALQGLKEEREQKNSLRRELSAYALNPFDSVGSLQLHLDELQLDDSREAEPDQDSGFNSGSGGPGCPPSSANGHHRYSTPRNSDVFLRPAPGLVSDLLSELNLSESQKLKQQLLQAEREKVALAGTVQDLQRQLEDAKQALSEQQGRSAALAEQLEALQGGADTLPQRQQGPRGEPESGADPEGGKDCPEAEHRLRTASEELAQLRRDLQEAEARSAGLEARHKQEKERWRAEAQELAEKIRQCIRSSRQDQERILELEREIGATRKVATDSEGHLTMAQDELVAFSEELANLYHHICVCNNMTPNRVMLDYYREGGRASRAHHGPLRRRRSSDLFGKMLFNPDGDSGGSGESSPVGGSPNSPTLDFRDPTNVRNLVAVIRNQIKHLQAAVDLCRQRGTLPCSPALGLPDTDRDSAALLEEVLKLKSLLSTKREQIATLRTVLKANKQTAELALSNLKTKYETEKSLVSETMVKLRNELKALKEDAATFSSLRAMFASRCDQYVTQLDEMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBB0</accession>
    <name>W5NBB0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of basal transcription 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBB0"/>
    <dbReference type="FunCoup" id="W5NBB0">
        <property type="interactions" value="1410"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017919"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017951.1">
        <property type="protein sequence ID" value="ENSLOCP00000017919.1"/>
        <property type="gene ID" value="ENSLOCG00000014561.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002062"/>
    <dbReference type="HOGENOM" id="CLU_054086_3_1_1"/>
    <dbReference type="InParanoid" id="W5NBB0"/>
    <dbReference type="OMA" id="TRKHNDF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014561">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="30054" checksum="159AAB120B56ADA2" modified="2014-04-16" version="1">EGEVPETKGEESGGEEEGPMSDDTGPAGGEAGQGLAGPGATGGERSAAGTKPSGRRAVPGVLYLGHIPPRLRPRHVRNMLSVYGEIGRIFLQPEGEDRHPAVAPELTQEAVARATVGWHCGPDVTDSPPVSRTLTPPRLPQYLHRFQWCHLSERLAYEQTVRQQRLRVEISQAKKETNFYLANVERSHSLEKLKKRKKKEGQSFLEKTWDFTQRPTEDEIRQRRLLKQNQPRGRSKKELAKALEKARAIQEKSQSNVSLLAKIFNTGASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBC5</accession>
    <name>W5NBC5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deubiquitinating enzyme A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C85 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01006602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBC5"/>
    <dbReference type="FunCoup" id="W5NBC5">
        <property type="interactions" value="564"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017934"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017966.1">
        <property type="protein sequence ID" value="ENSLOCP00000017934.1"/>
        <property type="gene ID" value="ENSLOCG00000014573.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158963"/>
    <dbReference type="HOGENOM" id="CLU_021938_0_1_1"/>
    <dbReference type="InParanoid" id="W5NBC5"/>
    <dbReference type="OMA" id="NKMHRDP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014573">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904515">
        <property type="term" value="P:positive regulation of TORC2 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22752">
        <property type="entry name" value="OTU_OTUD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000002">
        <property type="entry name" value="OTU domain-containing protein 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF4">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="10">
        <location>
            <begin position="162"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="513" mass="55358" checksum="FC326705793B8FCC" modified="2014-04-16" version="1">MTILPKKKPSSTVGVPDHPDESDRRSGPDSHPHSHQHPHPHTHGGRAGARPRASPGGRGELSGGVGCGAGVGVGAGVGSCCSGPGLSKRRRQAACAGGVAGGGVVGVAGGGGGGPSPDPEEGAGYNSEDEYENAARLQSLDPATVEQQEHRFEKALGEKKGFVIKKMKEDGACLFRAVADQVYGDQDMHDVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYGTDPEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPAFKPGYADQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLQDQEKQARQPRKASATCSSATAAASSGLEEWSGRSPRQRSSAPSPEHPDPHPDAAAKPPSPAGAALMLPKPPSPCAPGPSSQSCVGADRATPSPLVSLYPALEYRAIMQEMSPTAFGLTDWEDDEILASVLAVSQQEYLDSMKKNSMHREPSPDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBY2</accession>
    <name>W5NBY2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serpin H1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Collagen-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBY2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018141"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018173.1">
        <property type="protein sequence ID" value="ENSLOCP00000018141.1"/>
        <property type="gene ID" value="ENSLOCG00000014734.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156163"/>
    <dbReference type="HOGENOM" id="CLU_023330_2_0_1"/>
    <dbReference type="InParanoid" id="W5NBY2"/>
    <dbReference type="OMA" id="RHDHSKI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014734">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF27">
        <property type="entry name" value="SERPIN H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869136" description="Serpin H1" evidence="10">
        <location>
            <begin position="19"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="11">
        <location>
            <begin position="39"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="406" mass="45889" checksum="E0D2FE2E105B1A02" modified="2014-04-16" version="1">AMAFKAAVVSFLIGIVVAEPVALKAPTSVLSESTVNLGLNLYHTMVKDPSLKSENILFSPVVVATSLGVMSLGAKDKTASQVKSLLRVNLHEDKLHPAFSELFNDVSNETARNTTWKVGSRLYGPTSAELRQEFVEKSRKHYRHDHSKINFRDKRNALKSINEWAAENTGGRLSEITRDLPGSDGAMFVNAMHFKPHWGEAFHHKMVDNRGFLMSKTHSVSVPMMHRTGFYKYYEDEVNQLQIVEMPLGHRQTSMMIIMPFHVEPLERLEKMLTKEQLSVWSNKLQERAVAVSLPKINVDVNHELQKHLQELGLAEAVDKSKADFSGMTGKKDLHLSNFLHATAFDLDTDGNPFDQDIYGREEMRSPKLFYVDHPFIFLIQDKKTNSILLIGRLVKPNGDGNHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCA4</accession>
    <name>W5NCA4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 61</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCA4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018263"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018295.1">
        <property type="protein sequence ID" value="ENSLOCP00000018263.1"/>
        <property type="gene ID" value="ENSLOCG00000014831.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006479"/>
    <dbReference type="InParanoid" id="W5NCA4"/>
    <dbReference type="OMA" id="YWESWPT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014831">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000252">
        <property type="entry name" value="Leucine-rich repeat-containing protein 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF8">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="261" mass="29556" checksum="C2B665B458B024A7" modified="2014-04-16" version="1">PPSRIDKESETEFAKITAALLKSKTGEFDLESVLYLKLRCLGIYDLGCIGECINLERLDLSGNYITSLKPLASLKLLIVLNLSANRITNFEPICLCDNLQSLNVAGNLISSFENLQCLQSLRKLESFRLKDISCNYTNPVCKNTSYRNIILEMFPNIKVLDGERLAGRGSDLYQLCRDIDDSLKAGIYKNGPLPEVLDCKPWVEEGYWDLKRSNNAIIEEAYKQFNDILHECRLLSNRAAHLISQTERALSINSQPKQYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCL0</accession>
    <name>W5NCL0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01023927">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCL0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018369"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018401.1">
        <property type="protein sequence ID" value="ENSLOCP00000018369.1"/>
        <property type="gene ID" value="ENSLOCG00000014916.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="HOGENOM" id="CLU_009772_1_0_1"/>
    <dbReference type="InParanoid" id="W5NCL0"/>
    <dbReference type="OMA" id="PNNCSHD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014916">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF12">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="147"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="267"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="407"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="567"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="208"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="867"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="690"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="573"/>
            <end position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="894" mass="100006" checksum="47B73DBC36CF6935" modified="2014-04-16" version="1">SFPAPPSNWPRGLPVRRLPPQPCMNGTGFPPPDFGTAMGPGGLMSPVTPSPAGVSFIIQIGLTRESVLLPQSADLAYVKQMACSIVDQKFPECGFYGIYDKILLFKHDTGSNNILQLVKTASDIQEGDLIEVVLSAAATFEDFQIRPHALNVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCTGARKRRLSTTSLNSAQSLRLSTSESLSSSTPTEDSLPRTPSEVGTPARRFYSGRPIQLDKMLLSKVKVPHTFAIHSYTRPTVCQYCKRLLRGLFRQGLQCKDCKFNCHKRCAYKVPNDCLGETIINGDMLSPNVDPEVVMDFTSDFGDSDKSSLMDDSDDSGIIPGSHSENNFPEDSLSGTSCDQSAMGYIPLMRVVQSVRQTTRKSSTTIKEGWMVHYSNKDTLRKRHYWRLDSKCIILFQNDTTNKYYKEIPLSEILEVKAAENFSLVPPGTSPHCFEIVTGTMRYFVGEELSPCPAASSSITPNSGVGRDVAKAWEMVIRQALMPVIFQDAPTSQGHPPRRQASVSISVSNSQVQENVDIGMVYQIFADEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTRQESQLRNEVAILQSLRHLGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILAALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIHNAAFMYPPNPWKHISPEAIDLINNLLQVKMRKRYSVDKSLSHIWLQDYQTWLDLRELECKLGERYITHESDDRRWQQFAQDHTLPYPPHLIAPPAAGSDDSDDSDMQGLTERVSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCV2</accession>
    <name>W5NCV2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Activating signal cointegrator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Thyroid receptor-interacting protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction. May play a role in late myogenic differentiation. Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone. Interacts with the androgen receptor/AR; in an androgen, testosterone and dihydrotestosterone-dependent manner. Interacts with ESR1 (estrogen ligand-bound); competes with UFSP2. Interacts with UFSP2; competes with ligand-bound ESR1. Interacts with DDRGK1 and UFL1; the interaction with DDRGK1 is direct. Interacts with NCOA1. Interacts with EP300. Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3. Identified in the RQT (ribosome quality control trigger) complex, that contains ASCC2, ASCC3 and TRIP4. Interacts with NEK6. Interacts with CSRP1. Interacts with ZCCHC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCV2"/>
    <dbReference type="FunCoup" id="W5NCV2">
        <property type="interactions" value="1459"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018461"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018493.1">
        <property type="protein sequence ID" value="ENSLOCP00000018461.1"/>
        <property type="gene ID" value="ENSLOCG00000014993.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688354"/>
    <dbReference type="KEGG" id="loc:102688354"/>
    <dbReference type="CTD" id="9325"/>
    <dbReference type="eggNOG" id="KOG2845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005300"/>
    <dbReference type="HOGENOM" id="CLU_025737_1_0_1"/>
    <dbReference type="InParanoid" id="W5NCV2"/>
    <dbReference type="OMA" id="EFNSYRH"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014993">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061055">
        <property type="term" value="P:myotome development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001966">
        <property type="term" value="P:thigmotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000004">
        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056994">
        <property type="entry name" value="TRI4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056993">
        <property type="entry name" value="TRIP4_3rd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23135">
        <property type="entry name" value="TRI4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23134">
        <property type="entry name" value="TRIP4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ASCH" evidence="21">
        <location>
            <begin position="433"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="577" mass="65907" checksum="A72C6F2C05507DE5" modified="2014-04-16" version="1">MSDLLLRWCVDQLNRNFGLDACDDIVQYILSIENAEEIREYVGDLLQGTEGKKQQFMDELLDRWHRSRQPSSESIQLYHRKETVADVPEVLKGMDSLKKSKRKGRNKQEVPTVPEQEPAPEEVKTPIDLLKAQESSSSTTVKKKKFVNLYAQEGQDRLAVLLPGRHPCECLAQKHRLINNCLTCGRIVCEQENSGPCLFCGSLVCTKEEQEIIQRDSNKSQKLLKKLMGGGDRASQACGTEREFLPQQEARTKSGLEKALQHKEKLLEYDRNSVRRTQVIDDESDYFATDSNQWLPQAEREALRRREEELRELRHASRLGRKVTLDFAGRRVLEEGEGLSHYYSKLDETVQAISTGSLARTPTQPEQGTRQHLGELVNPNIMQAAPVWVEVGSSEVQRKARDKAGVTEPDSGRSRLRIQDRELQEIADGGWCLSMHQPWASLLIRGIKRVEGRTWYTSHRGRLWIAAAAKRPTPQETAQVEAMYRKLYRTAPDIPREYPTGCLLGCVNVTDCLSQEQYQKQFPDTCEESGSPFVFICSNPQELLVKFPMKGKHKIWKLDSQYHQAAKKGLMHQKSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDG3</accession>
    <name>W5NDG3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WASH complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Coiled-coil domain-containing protein 53</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC53 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025512">
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    </evidence>
    <sequence length="208" mass="21851" checksum="3D5BAAE5E907E32C" modified="2014-04-16" version="1">MDEDGLPIVGSGIDLTKVPAIQQKRIVAFLNQFIVHTVRFLNRFSTVCEEVSAAPLVLVVSLASRCSDVLCLCKLSSIPGLEDVRVERSAQRQVSEPNGPVSTPVQAPSSAVPAPTEPQQRPPEAAEQPRSGAAGDDVLTVAKDPRYARYLKMVQVGVPVMAIKNKMVSEGLDPSLLDTPDAAVPDAGAPGAGEEDDGSSGSESSFSD</sequence>
</entry>
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    <accession>W5NDU4</accession>
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                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31626" checksum="86281B5A2A441B08" modified="2014-04-16" version="1">RTWHLYIIRQEAAYYYSLETFAEHRAVCTRYQPHTYKILRTSGPKDREEPAPWDLSASPAKMFQNQVQYIRIPGTDVVKGCPGCRGQKWTPCSFCQASGKVRCPVCHGSGWSSKRRLCWGCNGQRLVPCAACMALGRVCCETCIGKGQLGYFQELRVEHKCNLGDHIHSTANIPGHLLPSAPGEVLYESTAEQLHGFSTSTVDEINSISQRLVEESRRTCRDCRIIQQRQMLKAVPVTQVQYYWKDKSGTFFIYGSDHCIYCTDYPKKKIICCTQWF</sequence>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
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            <dbReference type="Pfam" id="PF14952"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="629" mass="67782" checksum="38E021BEB2D621E0" modified="2014-04-16" version="1">MEGTGQGTGVPAAPVSAGPAPAASGSRERTKTPSFLSNLGKPTLRGIRKCPKCGMYNGTRGLSCKNKACGAVFRDGARKAQASAEAVRVVTGAGGGAAGGEAQVFSVRQRGRGPEQRGFVELGVTETAISTADGTLLTHISLGHCFVPSCRQGSAPGRGAPPAAQPAPPPQQERPCLHMKQAMECQAEATPLTLKSSVLGSLQAPAETKQALWQLATESLGPLVQRLSRAVMVVKCQRSARHPLGLLHLTLGERKDGLRYHCACQRAARPEGASRTALKLPEDPSSSSASPSPALADASSSCSQEYCLHLYACVCAFASDEKLAAEFSGFFSYDADGLHVGAECSVTNATNPPQPAERCTPHKPKRSKLEDSVAASIPTLPAKEGVPSSLRRFTSRKHPISTVLKKPGGAQPVDDEARVSLSFQHWLASVTERIHQTMHFQFGGKPDPLVFHIPQSFFNALQQRLSAGSKKKRLPNSTTAFVRKDALPLGTFSKYTWHINNILHVKQIFDTPEMPLEVTQSFVRNRDGSFEPFRCPEVPVEPETQSRADRQPSIRPLELRTFLRVGNTSAEQKDPTPFIIEWIPDILPRSKIGELRIRFEYGHLHNGHVEYSDSQGGPAPVEICRVTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE79</accession>
    <name>W5NE79_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Golgi transport 1Bb</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in fusion of ER-derived transport vesicles with the Golgi complex.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026453">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015352152.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5NE79">
        <property type="interactions" value="1296"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018938"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018970.1">
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        <property type="gene ID" value="ENSLOCG00000015390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697889"/>
    <dbReference type="CTD" id="100034417"/>
    <dbReference type="eggNOG" id="KOG1743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014507"/>
    <dbReference type="HOGENOM" id="CLU_124519_1_0_1"/>
    <dbReference type="InParanoid" id="W5NE79"/>
    <dbReference type="OMA" id="MWLTDAQ"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015390">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF79">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN GOT1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="141" mass="15623" checksum="C7984A8CF99F8413" modified="2014-04-16" version="1">MISLTDSQKIGMGLTGFGVFFLFFGMILFFDKALLAIGNILFVAGLSFVIGLERTFRFFFQKHKMKATGFFLGGVLVVLIGWPMIGMILEIYGFFLLFRGFFPVVIGFIRRVPVLGSLLNLPGISGFVDKAGESNNMIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NET8</accession>
    <name>W5NET8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Opsin 1 (cone pigments), short-wave-sensitive 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NET8"/>
    <dbReference type="FunCoup" id="W5NET8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019147"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019179.1">
        <property type="protein sequence ID" value="ENSLOCP00000019147.1"/>
        <property type="gene ID" value="ENSLOCG00000015553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691285"/>
    <dbReference type="KEGG" id="loc:102691285"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234549"/>
    <dbReference type="HOGENOM" id="CLU_009579_3_0_1"/>
    <dbReference type="InParanoid" id="W5NET8"/>
    <dbReference type="OMA" id="NFHLYEN"/>
    <dbReference type="OrthoDB" id="6142583at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015553">
        <property type="expression patterns" value="Expressed in camera-type eye"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008020">
        <property type="term" value="F:G protein-coupled photoreceptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016038">
        <property type="term" value="P:absorption of visible light"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000018">
        <property type="entry name" value="Rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001521">
        <property type="entry name" value="Opsin_blue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00574">
        <property type="entry name" value="OPSINBLUE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0157">Chromophore</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 17" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="5 17" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="15 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="49"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="346" mass="38478" checksum="90F395BCFD6CAAE6" modified="2014-04-16" version="1">MAGTEEFYLFENISSVGPWDGPQYHIAPKWAFYFQTIFMGLVFFWGTPLNTIVLIVTIKYKKLRQPLNYILVNISAAGLLAVFFSVFPVFIASSKGYFFLGKTVCALEGYSGAIAGLVTGWSLAFLAFERYIVICKPFVNFRFGSKQALIAVASTWAIGIGVASPPWFGWSRFIPEGLQCSCGPDWYTTGTKYKSEYYSYFLFICCFCCPLSLIIYSYSQIIRALKEVAAQQAESATTQKAERDVSRMVVVMVGSFLLCYGPYAILAMYMVNVRGGVQDLRLVTIPAMFTKSSCIYNPLIYSFMNKQFHACIMETVCGKPITDESEVSSASKTEVSSVSSSQVSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF69</accession>
    <name>W5NF69_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Prenylcysteine oxidase 1</fullName>
            <ecNumber evidence="10">1.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Only active against free prenylcysteines and not prenylcysteine residues within prenylated proteins or peptides. Involved in the final step in the degradation of prenylated proteins, by degrading prenylcysteines after the protein has been degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O = (2E,6E)-farnesal + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:30231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15894"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:62141"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:30232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[(2E,6E,10E)-geranylgeranyl]-L-cysteine + O2 + H2O = (2E,6E,10E)-geranylgeranial + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:70407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:189549"/>
            <dbReference type="ChEBI" id="CHEBI:189554"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70408"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an S-polyprenyl-L-cysteine + O2 + H2O = a polyprenal + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:53892"/>
            <dbReference type="Rhea" id="RHEA-COMP:13675"/>
            <dbReference type="Rhea" id="RHEA-COMP:13676"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:137934"/>
            <dbReference type="ChEBI" id="CHEBI:137935"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:53893"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the prenylcysteine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.5" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01031510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625658.1">
        <property type="nucleotide sequence ID" value="XM_006625595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NF69"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019278"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019310.1">
        <property type="protein sequence ID" value="ENSLOCP00000019278.1"/>
        <property type="gene ID" value="ENSLOCG00000015660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682350"/>
    <dbReference type="KEGG" id="loc:102682350"/>
    <dbReference type="CTD" id="51449"/>
    <dbReference type="eggNOG" id="ENOG502QSHJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011206"/>
    <dbReference type="HOGENOM" id="CLU_021176_1_0_1"/>
    <dbReference type="InParanoid" id="W5NF69"/>
    <dbReference type="OMA" id="SIGIWDG"/>
    <dbReference type="OrthoDB" id="437369at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015660">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001735">
        <property type="term" value="F:prenylcysteine oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030327">
        <property type="term" value="P:prenylated protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030328">
        <property type="term" value="P:prenylcysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000081">
        <property type="entry name" value="prenylcysteine oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010795">
        <property type="entry name" value="Prenylcys_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017046">
        <property type="entry name" value="Prenylcysteine_Oxase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944">
        <property type="entry name" value="FARNESYLCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944:SF3">
        <property type="entry name" value="PRENYLCYSTEINE OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07156">
        <property type="entry name" value="Prenylcys_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036292">
        <property type="entry name" value="Prenylcysteine_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0274">FAD</keyword>
    <keyword evidence="4 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867651" description="Prenylcysteine oxidase 1" evidence="17">
        <location>
            <begin position="19"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Prenylcysteine lyase" evidence="18">
        <location>
            <begin position="127"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="507" mass="56498" checksum="5CB435256BAF0852" modified="2014-04-16" version="1">MIILVPIRSLLLLTLCLSGRRGFASAPEHREAPKKIAVVGAGIGGTATAYYLRKEFGPDVKIDVFENGVVGGRLATAKIGGGEYETGGSIIHPLNLHMKHFVDQLGLSQRHDVPSRMAIFDGQQLVFEESDWFIVNFLRMLWRYGFNFIRMQMWVESILDKFMRIYQFQQFGYSFSSVERLLHAMGGGEFLRLTNQTLDEAMRGAGFSQQFLNDIVTPITRVNYGQSVKINAFVGVVSLAGAESGLWAVDGGNKLVCVGLLQHSRAQLIQGKVSSVTIKPRPLRTGGSVSLYEVNYATESGTAHNLYDIVIIATPLHKDKSDISFPNITPPVPSHFPGRYHQTVATLVHGRINVSYLGVKGPASAFWMSDILTMEHEGLVLNSLSSLDPVHIPQGYKRPPASLPNVWKVFSGKRLSKEQLGSVFLQWDEISEVPWLAYPSYSPPQRLPPIVLHERLYYLNGIEWAASAMEMSAIAARNVALLAHHRWHGMTHQIDQEDLHTRLRGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG80</accession>
    <name>W5NG80_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein MEMO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mediator of ErbB2-driven cell motility 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001617">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638637.1">
        <property type="nucleotide sequence ID" value="XM_006638574.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG80"/>
    <dbReference type="FunCoup" id="W5NG80">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019639"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019671.1">
        <property type="protein sequence ID" value="ENSLOCP00000019639.1"/>
        <property type="gene ID" value="ENSLOCG00000015951.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682800"/>
    <dbReference type="KEGG" id="loc:102682800"/>
    <dbReference type="CTD" id="51072"/>
    <dbReference type="eggNOG" id="KOG3086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006408"/>
    <dbReference type="HOGENOM" id="CLU_038085_0_1_1"/>
    <dbReference type="InParanoid" id="W5NG80"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015951">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.830.10:FF:000002">
        <property type="entry name" value="MEMO1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="33589" checksum="91D1864C2F33422D" modified="2014-04-16" version="1">MSNRVVCREASHAGSWYTASGPQLNAQLEGWLSQAQSTKRPARAIIAPHAGYTYCGACAAHAYKQVDPTVTRRVFILGPSHHVPLSRCALSPAEIYRTPLYDLRIDQKVYADLWKTGMFERMSLQTDEDEHSIEMHLPYTAKAMESHKDDFSIVPVLVGALSESKEQEYGKLLSKYLADPSNLFVISSDFCHWGQRFRYTYYDESQGEIYRSIEHLDKMGMSIIEQLDPISFSNYLKKYHNTICGRHPIGVLLNAVAELKKNGVDMNFSFLNYAQSSQCRNWQDSSVSYAAGALVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGW3</accession>
    <name>W5NGW3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sterile alpha motif domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts promiscuously (via SAM domain) with EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3 and EPHB4 (via SAM domain) (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGW3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019872"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019905.1">
        <property type="protein sequence ID" value="ENSLOCP00000019872.1"/>
        <property type="gene ID" value="ENSLOCG00000016128.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4384">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048701"/>
    <dbReference type="HOGENOM" id="CLU_115568_1_0_1"/>
    <dbReference type="InParanoid" id="W5NGW3"/>
    <dbReference type="OMA" id="VYTSHMV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016128">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09527">
        <property type="entry name" value="SAM_Samd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000055">
        <property type="entry name" value="Sterile alpha motif domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF8">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21941" checksum="965594BBB35DEF46" modified="2014-04-16" version="1">MNTGPNIVYEWLKTLQLCQYVESFVDNGYDDLEVCKQIGDPDLDAIGVFIPHHRQRIHDAVRRLKEEEKETDSGLYFTLEPMPPTAEIYTSHMVEQYETKLRGSKSWTEPSGDRAGKNQGNLSTSRNQHLGPCKELVTYPKLKLKIMIRDKLIRDGINLSKPPYSNKLNINNAKSLLFQKFVSSWHLSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHK6</accession>
    <name>W5NHK6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium two pore domain channel subfamily K member 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638693.1">
        <property type="nucleotide sequence ID" value="XM_006638630.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHK6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020115"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020148.1">
        <property type="protein sequence ID" value="ENSLOCP00000020115.1"/>
        <property type="gene ID" value="ENSLOCG00000016299.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693170"/>
    <dbReference type="KEGG" id="loc:102693170"/>
    <dbReference type="CTD" id="56660"/>
    <dbReference type="eggNOG" id="KOG4404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161424"/>
    <dbReference type="HOGENOM" id="CLU_022504_3_1_1"/>
    <dbReference type="InParanoid" id="W5NHK6"/>
    <dbReference type="OMA" id="MRACHER"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016299">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000070">
        <property type="entry name" value="Potassium channel, subfamily K, member 12 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005410">
        <property type="entry name" value="2pore_dom_K_chnl_THIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF11">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01588">
        <property type="entry name" value="THIKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="204"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="412" mass="45686" checksum="4965FFD8692C826D" modified="2014-04-16" version="1">MMPSQRMQGCRSLLHINEDNGRFLLLALLISLYLLCGAAVFSAIERPSELEAQSLWNRTFFNFSDTFNISLQDLSSFLRDYEAAIAAGIRADSLRPRWDFTGAFYFVGTVVSTIGFGMTTPATVAGKVFLIFYGLLGCAGTILFFNLFLERIITLLAVIMKAFHERQLRRSGLLPPTIRRGSAFSDSGSLAGWKPSVYYVMMILGLSAIILSCCASAMYTPVEGWNYIDSLYYCFVTFSTIGFGDLVSSQNAAYENQGLYRFGNFVFILMGVCCIYSLFNVISIVIKQVLNWMLKKMDCLCCRRCHKTNAFLGRRNAITPGSRLRRGHLPSETEGTCDSDTEGRRLSGEMISMRDLTASNKVSLAIMQKQLSETANGFPRTVCGSSRHNGFSGGVGALAIMNNRLAETSDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHR3</accession>
    <name>W5NHR3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:dkey-12h9.6</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Rough endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the macoilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHR3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020205.1">
        <property type="protein sequence ID" value="ENSLOCP00000020172.1"/>
        <property type="gene ID" value="ENSLOCG00000016341.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016613"/>
    <dbReference type="InParanoid" id="W5NHR3"/>
    <dbReference type="OMA" id="GQYQCQN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016341">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023041">
        <property type="term" value="P:neuronal signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019130">
        <property type="entry name" value="Macoilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464">
        <property type="entry name" value="MACOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464:SF1">
        <property type="entry name" value="MACOILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09726">
        <property type="entry name" value="Macoilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="503"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="660" mass="76346" checksum="B368A2943AA24FB2" modified="2014-04-16" version="1">MKKRFVDASRLRKMKRTKITERLSESAFTFLKFMIIWILVLLADFILEFRFEYLWSCWLFFGSVYTFFHCHGLVICVVFVCAAFTLDVFFLIFVPLHWLFFAASTYVLFNYIWHTEKGLCLSTVSLWLLLVYIEGSLRFKDLKNSHVNLSHLFAAHCIGYPIVYFGFDASCYFTSVVKLRIQKAVRNENDFYAQLLQQSLPPGLQFYPRLHLKRGRTLCMIILCNIVMNIIYCYASKWKTKFEFTQYQYRNGAAATDDKYSLDCLQIEGKEKREAEYAKPSDQNAQPPDPKAGENEHKQSSPNRTAISESCGSSECLPQEDLEAKAPKTSKSSSPKVRRTPGNSTMPIIKGEKKQRSTTKNTSPTRETGKQNISNNHNIYLEQIVRLEQELKKLKGELQSSKQNEQELRSHYCNLTNSERSLRPEIGLLRQENDLLQNKILCIMKSKQRDKQMCALLEKKIKAEMEARTIIEKQLSEMRTRKHDESSVIPQGALLSTNRQEHTETLKKKAKELETEYKQLQLEFQGKEGQIIALENEVEGLHKYKGIEQDADMLLTALSAMQDKTQHLEYNLSAETRIKLDLFSALGDARRQLEIAQGKILKQDQEIGEMKQKIAEVMAVMPGITYAVPHPITPQYLTKFLNSENYMLDPSALMYQCLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ12</accession>
    <name>W5NJ12_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial ribosomal protein S35</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ12"/>
    <dbReference type="FunCoup" id="W5NJ12">
        <property type="interactions" value="1243"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020621"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020656.1">
        <property type="protein sequence ID" value="ENSLOCP00000020621.1"/>
        <property type="gene ID" value="ENSLOCG00000016692.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003443"/>
    <dbReference type="InParanoid" id="W5NJ12"/>
    <dbReference type="OMA" id="TIRTDRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016692">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="2">
        <location>
            <begin position="163"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="38149" checksum="95F80CCC29C66FAF" modified="2014-04-16" version="1">MATSTMCTAASSLYRINCHFLGMHTLNPIYRGSVNSTARFLSTNTEKIGVTTRGSRGHLGQRSRREPGEPRTEKMAVDQDWTSVYPTAAPFKHGTVPLPVRMGYPVNRGVPPEKKGNLELIKIPNFLHLTPAAIKKHCAALKPFCTEWPEALDSSAKCEEHFPIQVKTTDYVSAGLSLRNPKARVVTLTVRLSSLNIDDHARKKLIKLVGDRYNKKTDMLTITADRCPLRQQNNDYAMYLLTVLYHESWKTEGWESEKTEADMEEYCWDNSTSQRNVLDTLLRMKGQEEASTAAQEELLKSKVVQDYGESMTKLKNEGESESTIRQYRESVKKLLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJQ4</accession>
    <name>W5NJQ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="14">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01011818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJQ4"/>
    <dbReference type="FunCoup" id="W5NJQ4">
        <property type="interactions" value="1907"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020863"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020899.1">
        <property type="protein sequence ID" value="ENSLOCP00000020863.1"/>
        <property type="gene ID" value="ENSLOCG00000016874.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00670000098098"/>
    <dbReference type="InParanoid" id="W5NJQ4"/>
    <dbReference type="OMA" id="ITHDMVV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016874">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="17">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="409"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="479"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="864"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="886"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="944" mass="108491" checksum="ABCBA108FA3AD5E7" modified="2014-04-16" version="1">MGVPAFFRWLSRKYASIIVHAVEEKAKECNGVRIPVDTSKPNPNEVEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFEYIDRLFNIVRPRRVLYMAIDGVAPRAKMNQQRSRRFRASKEGAELTEEKKRIREEIFQRGGYLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNSDPGWRNLTVILSDASVPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPQFTIIREEFKPNKPRPCGMCGQLGHEIKDCQGLAREKKGEHDEFADTPPASEQEFIFIRLSVLREYLERELTMASLPFPFDIERSIDDWVFMCFFVGNDFLPHLPSLEIREGAIDRLVGIYKDVVHKTGGYLTENGFVNLERVELIMQAVGVAEDNIFKKRREDEESFRKRMKEKKKRMKGGERPAFLPSGQFAPQALGGRNAPRPVHNARQAAFDMRMQHNQNLKLEPSTSFCTRSSDMADGRGVKRKAEDSDSEPEPEDNISRLWEEGWKQRYYKNKFDVDVTDEKFRRKVVKSYVEGLCWVLRYYYQGCASWKWYFPFHYYDQMGMVFDLFLTVVQPFKPLEQLMGVFPAASGNFLPQSWQRLMTEPDSSIIDFYPDDFAIDLNGKKFAWQGVALLPFVDERRLRAALEEVYPDLTPDEIQRNSLGSDLLFVGRSHPLSDFLKELYQTNSEESVEMSPELCHGISGTLTLDEDPVLPDKPVRSPIPVLRDVVENSAIGIKFKDPQYKEGFVFKATVLPGAKIPAKVLKPGDWEKSNRGPWRPQLGFNPDRRPAHLDQSAFRALSHNMSRDRKPGFYSNAPPPCAYSGFLYYLSMIFTQLKLPRLVNNSRQQGSLLGAPHGFSQQQYPRQYGSDASRGWSQGMPSTGAAFQQNQYRGRGGPSGYQPHQDRRDNRGRQGYNSGRNYPSQPPAGRYNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKI8</accession>
    <name>W5NKI8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Diacylglycerol kinase</fullName>
            <shortName evidence="23">DAG kinase</shortName>
            <ecNumber evidence="23">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="22">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 23">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="23"/>
    <dbReference type="EMBL" id="AHAT01035331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035332">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021147"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021183.1">
        <property type="protein sequence ID" value="ENSLOCP00000021147.1"/>
        <property type="gene ID" value="ENSLOCG00000017111.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0782">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158094"/>
    <dbReference type="HOGENOM" id="CLU_003770_4_0_1"/>
    <dbReference type="InParanoid" id="W5NKI8"/>
    <dbReference type="OMA" id="XIPLGIL"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017111">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046339">
        <property type="term" value="P:diacylglycerol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF92">
        <property type="entry name" value="DIACYLGLYCEROL KINASE IOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 23" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="24">
        <location>
            <begin position="142"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="656" mass="73177" checksum="6821EBDDE91713FC" modified="2014-04-16" version="1">RLLQISFRCKPTFREGGSRCLRDNFTIHHRTHXLTWNCLICFLSPPQSFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVKKPQVRDVPVGRGDFSDFGVQSWGFVMFVPVSVTSGMVGDLLLLLLPYQGTKVLQSFMWILNPRQVFDLSQGGPKEGLELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGNVVQLDRWNLEVQRGSARRLGDQEEGVHKVGSTPLSVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLVSIAHEARNRDIQGSAEMFLPASHKYSPTSGDARYCRRAPDCIGFLTSNPLGYLEQKGQKRKVFPLLGEQVDVLDEIFTVDINPLSTVLDSGLRPSLAPIGSCSCPESAHVLLCLPPPAIPVGIIVVRGDCDLETCRLYIDRLQEDLYLAPSSTHRVHYQDESRGLPWSQSAHSLSANWSFLDCKSSGDYYRITRNLEHLHFVTEISQDEIFILDPEVLGQPSGGPGMPDVVVEPSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMD0</accession>
    <name>W5NMD0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rapunzel 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192199.1">
        <property type="nucleotide sequence ID" value="XM_015336713.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192201.1">
        <property type="nucleotide sequence ID" value="XM_015336715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMD0"/>
    <dbReference type="FunCoup" id="W5NMD0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021789"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021826.1">
        <property type="protein sequence ID" value="ENSLOCP00000021789.1"/>
        <property type="gene ID" value="ENSLOCG00000017684.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698790"/>
    <dbReference type="CTD" id="550609"/>
    <dbReference type="eggNOG" id="ENOG502QS3S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008158"/>
    <dbReference type="HOGENOM" id="CLU_703924_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMD0"/>
    <dbReference type="OMA" id="DREARTN"/>
    <dbReference type="OrthoDB" id="8549910at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017684">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039051">
        <property type="entry name" value="SE-CTX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472:SF9">
        <property type="entry name" value="RAPUNZEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472">
        <property type="entry name" value="RICIN B-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="44698" checksum="9C1D3945324AA0A2" modified="2014-04-16" version="1">MTNQLQKMVAEKKDVVETVMEVFEQGAEVVASMAGDLFPIFQIAAPIVKLALDNVESEEAAYMKEQFQKVRERLEVLSEEIKRINEEIKKSQVDAEYFSVEENLTNQFRKYMDILNAKPKFKEVKKKLFLEHFSKTGGDKNLHTLYNAVTGDNFSGESILEITLNYEEKSRRPMEEFCARLKKLFCVGLIALMGHAALKGDDEEALLKDWGEKMKAVQGKMNAVIEDCISSFPEQAKMDARKLVRDREARTNQQLADHLVDFLKKKYDWVSWSVRVFNSPSGVFSNMFSKEYQCCTGRSRFQLPEGDSKFNVVVSYSASPEPVDKSDIQQLIEQQKKASVVSVAELLFDRLPACVVHAVKPTKDLGVSWSFSEELHYWEQHKNFAVCVHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP12</accession>
    <name>W5NP12_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C-C chemokine receptor type 4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP12"/>
    <dbReference type="FunCoup" id="W5NP12">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022371"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022412.1">
        <property type="protein sequence ID" value="ENSLOCP00000022371.1"/>
        <property type="gene ID" value="ENSLOCG00000018270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078555"/>
    <dbReference type="KEGG" id="loc:107078555"/>
    <dbReference type="CTD" id="100329767"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230359"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_3_1"/>
    <dbReference type="InParanoid" id="W5NP12"/>
    <dbReference type="OMA" id="CKVVLGI"/>
    <dbReference type="OrthoDB" id="8951197at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018270">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14984">
        <property type="entry name" value="7tmA_Chemokine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000026">
        <property type="entry name" value="C-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF627">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="50"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="351" mass="39487" checksum="41D6CEB456A10158" modified="2014-04-16" version="1">MSSAMEAEYDYNNSLGIDYKEEFAPCKYEKHGAAFLPAVYSVLFVVGIIGNALVLWVVLLHVKLKSMTDVCLLNLAIADLLFVFSLPFLAHNARDQWVFGDPMCRIILGSYNIGFYSGIFFITLMSVDRYLAIVHAVYALKARTAIYGGIASVVTWIVSILASFPEIMYITVNLEEEKSRCLPLYFENDKQPLRVFSAFKLNVLGLLLPSVIMVFCYSMIIRRLLTSMSAKRHAIRLVLCVVMVFFFCWTPYNIACFFKGLQLLDIISSCPSSKKIILSLQITEIVAYSHSCLNPFIYVFVGEKFKRHLSRLVAGGPCKNCKILKKYMTPTTGSVYSQSISMGDNSVLSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPA5</accession>
    <name>W5NPA5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA binding motif protein 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPA5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022464"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022505.1">
        <property type="protein sequence ID" value="ENSLOCP00000022464.1"/>
        <property type="gene ID" value="ENSLOCG00000018363.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689428"/>
    <dbReference type="KEGG" id="loc:102692368"/>
    <dbReference type="CTD" id="8904"/>
    <dbReference type="eggNOG" id="KOG4307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160611"/>
    <dbReference type="HOGENOM" id="CLU_004368_1_0_1"/>
    <dbReference type="InParanoid" id="W5NPA5"/>
    <dbReference type="OMA" id="IIMNAEA"/>
    <dbReference type="OrthoDB" id="2588702at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018363">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12512">
        <property type="entry name" value="RRM3_RBM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12749">
        <property type="entry name" value="RRM4_RBM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12751">
        <property type="entry name" value="RRM5_RBM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000193">
        <property type="entry name" value="RNA-binding motif protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034855">
        <property type="entry name" value="RBM12_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034856">
        <property type="entry name" value="RBM12_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034854">
        <property type="entry name" value="RBM12_RRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="308"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="440"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="555"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="817"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="718"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="774"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="792"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="893" mass="94633" checksum="8A0C2493F7019DCC" modified="2014-04-16" version="1">MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGEHGEAFIVFATDEDARLGMMRTGGTIKGSKVSLLLSSKTEMQNMIELSRRRFETGNLEAPAGNGSRPGPPASSGGSGRGSLPTTMQGFSSPTPSVVTTAAASAHEPVGSKSIPTFSTASMSTIPPSYSNNFSSPSLTMGSTMTTAISTLNSVPPPMPPLPTLPSLPPMPSIPPMPVPPPVSTLPPVPPSTPIPPVPPVPPITHMPPMSTMPPFNPGVPPPVGPVPSGLSGSGPPMSMGNPNHMFLGPMNPLGPLNPQAHLKPPVVNPDELYVHLQGLPFSATEMDVRDFFHGLGVDSVRLLRDHLGRSSGRALAKFFMPQDAFEALKRNAAMMGQRYVEISPATERQWLASGGASSYSGGQGQSKHGSDHSDQDHHRRLRSSSESPSGRDRARSRSPHKQEFCVYLKGLPYEAENKQIFEFFKNLDIVEDSIYIAYGPNGRATGEGFVEFRNEMDYKAALGCHMQYMGSRFIQVHPITKKGMFEKIDVIRKRMQGTQQGDHKTGSFEGGGDKSSSRSCAHISNIPYNVSKKDVQLFLEGIQLFEDSLKVLVDGNGQGLGQAVVQFRSEEEALKAERLHRQKLNGRDAFVHLVTFEQMKEIERNPPPQVKRGQKVQLPQAHSYPSVSGDEYAFLRGSSGSITNGPPFGTLYSAPGNGLGGPPPLPPVGVGGGLGDVPISVPPPVVPGHLPSPMLEPPGFRPGPSSTSGPPVFPGGPESMRGLPPFDNGPRKSSGPPSRGGAGGGGQGRAGGSQSGYGPPSSEALRGPPSLSGGPGSQRPTIVKIQNMPFTVTVDEILDFFYGYQVLPGSVCLQFSDKGMPTGEAMVAFESQDEAMSAVLDLNDRPIGSRKVKITLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVC4</accession>
    <name>W5LVC4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LVC4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000081"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000081.1">
        <property type="protein sequence ID" value="ENSLOCP00000000081.1"/>
        <property type="gene ID" value="ENSLOCG00000000072.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154370"/>
    <dbReference type="HOGENOM" id="CLU_015718_4_3_1"/>
    <dbReference type="InParanoid" id="W5LVC4"/>
    <dbReference type="OMA" id="WIIILEL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000072">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000031">
        <property type="entry name" value="tubulin beta-4A chain isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36527">
        <property type="entry name" value="OS01G0282866 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36527:SF3">
        <property type="entry name" value="OS01G0282866 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="134" mass="14825" checksum="A3E4A6F039194C99" modified="2014-04-16" version="1">RIRLVIYSAVLLKTPFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW76</accession>
    <name>W5LW76_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Centromere</location>
            <location evidence="4">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="W5LW76"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000383"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000383.1">
        <property type="protein sequence ID" value="ENSLOCP00000000383.1"/>
        <property type="gene ID" value="ENSLOCG00000000351.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153472"/>
    <dbReference type="HOGENOM" id="CLU_004962_0_9_1"/>
    <dbReference type="InParanoid" id="W5LW76"/>
    <dbReference type="OMA" id="THECAAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000351">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000298">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="13">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="148" mass="17142" checksum="CDC52F457F2F3AC8" modified="2014-04-16" version="1">MDQLCVFSVSGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIIDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWK9</accession>
    <name>W5LWK9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein phosphatase 1 regulatory subunit 32</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWK9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000516"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000517.1">
        <property type="protein sequence ID" value="ENSLOCP00000000516.1"/>
        <property type="gene ID" value="ENSLOCG00000000471.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107076333"/>
    <dbReference type="eggNOG" id="ENOG502QR8X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287309"/>
    <dbReference type="HOGENOM" id="CLU_1712672_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWK9"/>
    <dbReference type="OMA" id="THRHFLP"/>
    <dbReference type="OrthoDB" id="9980630at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000471">
        <property type="expression patterns" value="Expressed in testis and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031410">
        <property type="entry name" value="SAXO4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15691">
        <property type="entry name" value="PPP1R32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="153" mass="16641" checksum="491F415049D5E6CF" modified="2014-04-16" version="1">MMARVAVGRPCPRVLASVGADVNPLSFYSTSYSASYGTGRFVPRPGRHSGTGYAANLRPVLHYQPSLDRTDNPPLGLSLLDSLQSVTHRHFLPLCRPDGSEPLPQIRGKRESAYLRDTAVERGGKMLHQSEYQGHFVPLATGPAGPGRILILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXI8</accession>
    <name>W5LXI8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Large neutral amino acids transporter small subunit 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639489.1">
        <property type="nucleotide sequence ID" value="XM_006639426.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXI8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000845"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000849.1">
        <property type="protein sequence ID" value="ENSLOCP00000000845.1"/>
        <property type="gene ID" value="ENSLOCG00000000750.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682401"/>
    <dbReference type="KEGG" id="loc:102682401"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155581"/>
    <dbReference type="HOGENOM" id="CLU_007946_3_0_1"/>
    <dbReference type="InParanoid" id="W5LXI8"/>
    <dbReference type="OMA" id="YMAAWSW"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000750">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015804">
        <property type="term" value="P:neutral amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000003">
        <property type="entry name" value="Y+L amino acid transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004760">
        <property type="entry name" value="L_AA_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00911">
        <property type="entry name" value="2A0308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF519">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="356"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="503" mass="55432" checksum="20E184ED8FC4B285" modified="2014-04-16" version="1">MTELKSRRGESMKKEKEEMEEMEVREGNSPDGSTSKIELKRNITLFNGVGMIIGTIIGSGIFVTPTGVLKQAGSVGLSLIIWVVCGVYSTLGALCYAELGTTITKSGGDYTYILEVYGSLAAFLKLWVEMLIIRPSSQYVVSLVFATYILKPFYPDCGVPDLAAKLIACLCLTLLTFVNCISVKAATKVQDLFTASKLLALGIIIIFGFIQIAGGDVQYLKKDQVFEGSKTGVDNIVLALYSGLFAFGGWNYLNYVTEEMVNPERNLPLAIIISMPIVTIVYVLTNLAYFTTISPYEMVETEAVAVIFGEYHLGVVSWLIPVFVGLSCFGAVNGSLFTSARLFYAGAREGHLPAALGLVHTDLFTPVPSLIVTCFLSMLYACSNDIFSVINLFSFFTWLCVGMAIAGMLWLRWKKPELNRPVKIIIVVPLVFVLGCVFMIAVSFWAAPFECLIGMGIILTGVPFYFLGYKWKKPHVIEKVIEIFTLFCQKILMSVPEEKREAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXK4</accession>
    <name>W5LXK4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">BR serine/threonine kinase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXK4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000861"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000865.1">
        <property type="protein sequence ID" value="ENSLOCP00000000861.1"/>
        <property type="gene ID" value="ENSLOCG00000000765.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157462"/>
    <dbReference type="HOGENOM" id="CLU_000288_156_2_1"/>
    <dbReference type="InParanoid" id="W5LXK4"/>
    <dbReference type="OMA" id="DTHCVPV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000765">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000064">
        <property type="entry name" value="BR serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF108">
        <property type="entry name" value="BR SERINE_THREONINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21122">
        <property type="entry name" value="KA1_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21115">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="345"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="421"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="690" mass="77370" checksum="5B9E3769D8EBD0DB" modified="2014-04-16" version="1">MSGKDSTGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRNLLEKVKLGVFHMPHFIPPDCQNLLRGMIEVDATKRLTLEQIQKHTWYIGGKNEPEPEQPIPRKVAIRSLPSADDIDPDVLESMHSLGCFRDKNKLMKDLLSEEDNQEKMIYFLLLDRKERYPSHEDENLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQSKAVFSKSLDISDANPKFNKEERSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPASTPNPTPPSSPSIGGMPWRTRLNSIKNSFLGSPRFHRRKLQVPTQEEMSSLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKSTGGPTVFQKPVKFQVDITYTEGNEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSVQQLSDTTHCMELLTGRLSKCGIVQKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXY3</accession>
    <name>W5LXY3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:cabz01093077.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
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                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <sequence length="2119" mass="240772" checksum="4E166C9BD7F8BC32" modified="2014-04-16" version="1">MDALQMLVDEVALEGLDGVTIPALWLRVENRIPKFPLNLDPASKQFLWQSLVCNPEIEFYELPEKRPPLVLVDRFKDIDPETGIQEIRRDMGSSEDIYPVHIVFDNKHGIKGSCRFFNERVNITSRIRSADLQPCCTLEDAFQRWDERLVLVASQRVRFRALIGWEGDPDLTLPDYSYCILERLGRSRWQGELQRDLHCKAFKTDARKLHYLRKVLDRNGLVTLQSHVIRLPSGSQQHSVLLLLKRFHIDRRSKYDILMERVSNVLAAKPDRMAIMNGLKEEVGVSEKTFKRMYQYMCTAGLAQTVTVPLQELNPQGGPCKTKKGTDVTVRCLKLLKEYRKKVEDDEDDNEEDDGVRKSAQSSGRFWERDMLTQAFEIIENRGTKGISQAEVRRHMNIGKLEARMICRLLERYELIKGFMEDEGRQRTTKYISRTHVGQSSLIQQFEKEKARSEELTTGSGCAARRAQPKGALSPGHSSAEEEEEGPGATGGRATRRRVLKVNTRSLFRKSRRHHAVQHSTPNKKLNIPALKRRRDNLDLAEATIGKEDIHATLDNSDISMEFSEQAANQSSSFHSVDNEGSVTVIEEVTMDQSKDLDVVPKKKSKSKSQERPHETYRLLKRKNLIIEAVRSLKLIENLYTLQKMIMDEEKQDGVVTKCCKKSILRLVRKLSQEGLLRLYRTTIIQDGVSKKVEFVVHPSILQDDPLVKSAIEQIRFRISSSYSAHRVKPGLAQTESAESGPARDGGDQEREGLPTPEELRKGEGSSGAAASKTDEKMGVSQLKNFQPTIIPGLGRSLGFQPKMLRLRLVHLFLWYLMYGHPLRDESQSPKPLAPEGRDEEPGHGSPASQGDPQSAAASQEAASQGVSEGKEAAKESECPSGTDGGAEKDQSSVDKTVYVDDLSWKRYIPPTPIHREFGYGWALTSDILLCLPLSIFIQIFQVSYKVDGLEDFLNDPIKQHYLVRFLPGKMKKQLLYKRKYIFSFYESMQRLCYMGLLQFGPSEKFQDKDQVFIYLKRKATIVDTTSCEPHYNLVRGNRPFERRFYTFNTLQDVKNFWFDMQCVCLNTPLGVVRWPRAKKSSAPEDQINTADPAMETKFSAEKQSSKDLSRMIELARGSREVTDEGLIPGDGQGAAGLDSSFFSHLKRNWIWTSYILNKTKSGSGTLDGRPTLRLKTFLRKHPLPLSFESSVVNRIVESRLLDVMKEDEVLIENQCSDTRNMRVRGGRNQKRKRLKKSTEKKGKKKTKEEKKDAQRRRQPRFHDEADRSALQRMTRQRVCWSAQEDGFLMLCRVASHVLNRKIKKPFVPWQVVRDLLHSQFEESLDKTSLSVGRRSRYIMKNSQTYLNFKICLAEVYQDKPLADEFVNRNNDYENPEVCAAEFKEFVEVLRQKFSSSAGTSGLKIPDTKEELFRRFSVYVIEDETEREIQQDILTSQEDIHAIVLNNLIQSTLALSNLQMKSCRSFQTFHLYIQYRDEVLCQAFLKCQQRGLVNRRRVSCRSGPKKSRALPFVPMSYQLSQSYYRCFTWRFPSSLCTESFQFLERLRSAGREDRANTFSFQDQEAADSEPGMVVFSLDALGGHCLASLSLFILGLLSVEVSIPEQIVVVDSTMVDNEVVKSMGKEMDEEDEDEAEECESKRKIEVKARQASHTNYLLMKGYCAPGIVSLRNLNPNDNIVVNSCQMKLKLRCTPAHSHFLPAAPSFLDEIVHGEACLPSSFTRLLTRSDDSNRLERFQSWCIDRCGYSPEDLQAVLDVCSAIEDSRFYGCDKLQLRRRFAGFEEKGDGRTRSLCQYLEDLISLEEIIEVGGNTVRLVAFKYLDPWVLHVNNSITPKDKAVSGDPSGARKRKLEEPCLEKGPPPGKVPHLESDSEVTVDTVHSEEQGQDQSLRTSSCDRADPSQSTEECNNAPQARGIDEESQNNKDHLGDTEMEEGSSVLDGAVSSSTDQMSASHEGAASKPNVGGGPTDDDDPCSTTSETVGDRGERGRVSFVSRPWRVVDGTLNRPVCKGMLEALLFHVMSRPGVPEGVLIEHYSGVLQPVVFLELLQGLEEMGCVRKRHIKREAHASLFSRQDFADQQEEQLQPGTASTVFYEPSVDCCLRLGRVFPSEPNWNKWVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYZ7</accession>
    <name>W5LYZ7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vimentin-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYZ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001354"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001359.1">
        <property type="protein sequence ID" value="ENSLOCP00000001354.1"/>
        <property type="gene ID" value="ENSLOCG00000001192.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156146"/>
    <dbReference type="InParanoid" id="W5LYZ7"/>
    <dbReference type="OMA" id="DMEERHG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001192">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF5">
        <property type="entry name" value="VIMENTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="95"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="139"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="301"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="461" mass="53235" checksum="42E2FF28C71FF26D" modified="2014-04-16" version="1">NDHKMSSVTTRQSSYKRMFGGERSIAPSTSFSSRRYSSPIRSRVSYSMSAPPAIYATNTSRLRSSATMPRLSTETSDFSLSDAVITDFKASKSNEMAEMQHLNDRFVSYIEKVCFLEQQNKILLAELEQLQGKGISRVGDLYKEEMRELRGQVDQLTTDKARVEVMRDNLDDDLQRLREKIQDEMRLREEAENNLQSFRQDVDHASLARLDLERKVESLQEEIAFLKKLHDEEVAELQAQIRHQHVQIDMDVVKPDLTVALRDVRTQYESLASKNIAEVEEWYKSKFADLTEAAARNNEALLQAKQESTEYRRQVQALTCEVHALKGTNESLEHQMHEVEENFALESSRSQDTIAHLEVDIRNMKDEMARHLRDYQDLLNIKMALDMEIATYRKLLEGEETRIATPGPMFSSLNLRGKQNLPNSEEDQNMNHAKQTFISLTRGSRVMQVIKEATQHHDELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZJ1</accession>
    <name>W5LZJ1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 184a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01032363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZJ1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001548"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001553.1">
        <property type="protein sequence ID" value="ENSLOCP00000001548.1"/>
        <property type="gene ID" value="ENSLOCG00000001359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153861"/>
    <dbReference type="HOGENOM" id="CLU_012923_3_0_1"/>
    <dbReference type="InParanoid" id="W5LZJ1"/>
    <dbReference type="OMA" id="WPFNRWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001359">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="418" mass="47179" checksum="A0D4D1143137346D" modified="2014-04-16" version="1">EEMPAGFVTANLSYAKDSRSQPLSSVLSDDTDRRNGTVLISKNSTISEDQLFLNTAAAQVLSGIFVWSALLLTCHQIYMHLRSYTVPNEQRYIIRILFIVPIYAFDSWLSLLFISNGQYYVYFDSVRDCYEAFVIYNFLSLSFEYLGGESGIMSEIRGKPIQSSCLYGTCCLGGMSYSIGFLRFCKQATLQFCVIKPIMAVITILLQAFGKYHDGDFNVNSGYLYITIIYNFSVSLALYALFLFYFATSDLLRPYEPVLKFLTIKSVIFLSFWQGMLLAILERCDVIPKALYIDGHKVGAGTVAAGWQNFIICIEMFFAAIALRYAFTCRVYQEKKSDTPDNIAPMQSISSGLKETINPNDMVQDAIHNFSPAYQQYTQQSTQEVDQPHQNGKTGSGGGVKRPKRNDKMMLITSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZV5</accession>
    <name>W5LZV5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fignl1 interacting regulator of recombination and mitosis</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZV5"/>
    <dbReference type="FunCoup" id="W5LZV5">
        <property type="interactions" value="649"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001662"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001667.1">
        <property type="protein sequence ID" value="ENSLOCP00000001662.1"/>
        <property type="gene ID" value="ENSLOCG00000001436.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691023"/>
    <dbReference type="KEGG" id="loc:102691023"/>
    <dbReference type="CTD" id="55732"/>
    <dbReference type="eggNOG" id="ENOG502QQPV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004791"/>
    <dbReference type="HOGENOM" id="CLU_335441_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZV5"/>
    <dbReference type="OMA" id="PCVQQTF"/>
    <dbReference type="OrthoDB" id="6088000at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001436">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027902">
        <property type="entry name" value="DUF4487"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16071">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16071:SF2">
        <property type="entry name" value="FIGNL1-INTERACTING REGULATOR OF RECOMBINATION AND MITOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14868">
        <property type="entry name" value="DUF4487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="907" mass="102224" checksum="FA17D325C94AD42F" modified="2014-04-16" version="1">MSQVTLLEEITQWSVEICQQELRSVLPKLLSIYHHTADWDEYIRVLKILTQMFLPHLSLTELEGEFFSKVLPKVVKMFNDLTEEISNQVGELSSQNIELRVLLRNILQIMVQVLEALSGCVRHICSFEENLASFDSIRSLPSCILKILKNTFQHCKESETAYQGRLSLVADLLQALFKEAYSLQKGFMELLDRISLGNSATEEEVSDMVTVIHSLLDICSVISNLDLAMHANTWKFIVKQSVKYQVLLEDQLRHSDIVSSLCDDVLASFHSCMELAEQMKQSGVQESSQSAECKFFQKTAKICRFFANTLVHYIKEFQPFLSKSCGQFHRLYLQIYSKFPPSLSSPVISERHCNEISNVLVPMDPFITQLLPFRPFAEAVLAEEQQSCPELCLPQCLLLVNILGKLPSLPEDVLDLWCQGSQFPEETPRLSVFQALFLSFQRCSLERAIPILLPGVMMNGQAQSKVTLYQHVCIQLSACIASLPPAHFSQLEHSLLGALLSSDVQTALLATDVWCFLARFGTAELCQHHGMIIAHLIKSCPGDCYQLSHLALLLRRMIFLMTPNHQVQFISKFSPKEAENLTLWQHVLLKALSLDVRKQVEEDIMKSATAVCASWMEEGCKLGQLEKVNTALSAMLSVRQGSGQVLESEAEQSVSRLVTQLWPRISVKQVLTQPALQCTLRPLLCLSATAVQCIDHQVICQALTCLTSLLPLKCPDDIVLAALDLLASLGRLFIPPEIQSQVLPKLSSLFSSLLAEGSWLLHQCTLEAFAQFAEVTNHEHVIPESLASEETKNKVVKFLSKAVHLEENKEERLERLKSERCVIERHRHRLETGAVEVTGDAEPCPKRPRQETCEEEQYEMVLQTAENALKTLQSLTEQTPPPQWVAARLQSLQTQITHINQVKLKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0F9</accession>
    <name>W5M0F9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interleukin-1 receptor-associated kinase 4</fullName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="W5M0F9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001866"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001871.1">
        <property type="protein sequence ID" value="ENSLOCP00000001866.1"/>
        <property type="gene ID" value="ENSLOCG00000001620.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158792"/>
    <dbReference type="HOGENOM" id="CLU_000288_21_15_1"/>
    <dbReference type="InParanoid" id="W5M0F9"/>
    <dbReference type="OMA" id="PSVHKMY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001620">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08793">
        <property type="entry name" value="Death_IRAK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000028">
        <property type="entry name" value="Interleukin 1 receptor-associated kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000414">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017428">
        <property type="entry name" value="IRAK4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037970">
        <property type="entry name" value="IRAK4_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038189">
        <property type="entry name" value="IRAK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="180"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038189-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="458" mass="51704" checksum="03B89B49026A81EB" modified="2014-04-16" version="1">MSKTITPSTYVRNLNHGMFRKLADFLDPQEDWKKIAVNIQKPSGEPRYNQFHLRRFEQQAAIGKSPTSELLYDWGTTNCTVGDLVDILVQNQLLSSASLLLPGKWNLVIAVLQYAVKEAPVAIRDSQEAQTIPVGRTVESYAPDSTTQPEEEDFDGQGFSRFSFQELRKLTCNFDERPVSEGGNKLGEGGFGVVYRACIDKKVVAVKKLTMISDLSLEELKLQFKQEIQAMKMLKHENLVEMIGFSYDGDHPCLVFDYMSNGSLLDRLACLDGTPPLPWSKRCSIAVGTARGIEYLHTNHHIHRDIKSGNILLDKMFVAKISDFGLSRASAKLTSTVMTETIVGTTAYMSPEALRGEITPKSDIFSFGVVLLEIISGHPPVDENRDPKFLVSWKDEIEDEEISLEEFVDKKIKDWDFSSVENMYFLASQCLNERKNKRPEMKEVHEQLEDIQNCTSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1P3</accession>
    <name>W5M1P3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neuron-specific calcium-binding protein hippocalcin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-binding protein that may play a role in the regulation of voltage-dependent calcium channels. May also play a role in cyclic-nucleotide-mediated signaling through the regulation of adenylate and guanylate cyclases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomer; oligomerization is calcium-dependent. May interact with the voltage-dependent P/Q- and N-type calcium channels CACNA1A and CACNA1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1P3"/>
    <dbReference type="FunCoup" id="W5M1P3">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002301"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002306.1">
        <property type="protein sequence ID" value="ENSLOCP00000002301.1"/>
        <property type="gene ID" value="ENSLOCG00000001992.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158110"/>
    <dbReference type="HOGENOM" id="CLU_072366_1_2_1"/>
    <dbReference type="InParanoid" id="W5M1P3"/>
    <dbReference type="OMA" id="QMYDPAR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001992">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000078">
        <property type="entry name" value="Hippocalcin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000072">
        <property type="entry name" value="Hippocalcin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF57">
        <property type="entry name" value="NEURON-SPECIFIC CALCIUM-BINDING PROTEIN HIPPOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867464" description="Neuron-specific calcium-binding protein hippocalcin" evidence="14">
        <location>
            <begin position="26"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="252" mass="28572" checksum="5533E402C19288DC" modified="2014-04-16" version="1">MRAVEGCGLVLVLVLGCLLLRPVPAAESAGSQAPWEAGDVSDRCEHPLVLLPCTQRRTAMGKQNSKLRPEMLQDLRENTDFSDHELQEWYKGFLKDCPSGTLNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNNDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISREEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDLNNDGKLSLEEFIKGAKSDPSIVRLLQCDPSSASQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2Z6</accession>
    <name>W5M2Z6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">VPS37D subunit of ESCRT-I</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2Z6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002754"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002760.1">
        <property type="protein sequence ID" value="ENSLOCP00000002754.1"/>
        <property type="gene ID" value="ENSLOCG00000002350.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183012"/>
    <dbReference type="HOGENOM" id="CLU_079658_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2Z6"/>
    <dbReference type="OMA" id="WQFQGLQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002350">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000018">
        <property type="entry name" value="Si:ch211-284f22.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF24">
        <property type="entry name" value="SI:CH211-284F22.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="10">
        <location>
            <begin position="89"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="85"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="33318" checksum="662197E18775E2D0" modified="2014-04-16" version="1">MSRGTREQNSNPDGFLVLGTSELRDLLDDEDKMHQIVNLDEIFQELQVDRETMLTTNRSLAEESLSRQPGLQNGKLQLADKYRKLEHLATVCQEKQRRLEAYIQKRSPQVARTLLQEEVSRAEEESEDLLEKFMEGSLPLEGFLDSFQSIRKICHIRRAQAEKIQELLKPNQNLRKPKNSGEEKKTAQQEPPSNGFSAQGPPRVFQLRYGLTPAILLPHFPLPSTSAIAHAASLPPLDSQPGQTQALGPSAPHSIPGQPVGLRVIGQLPGWPARPVRLQQICRQHNHHQPEPPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3D2</accession>
    <name>W5M3D2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012266">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3D2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002890"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002896.1">
        <property type="protein sequence ID" value="ENSLOCP00000002890.1"/>
        <property type="gene ID" value="ENSLOCG00000002412.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157045"/>
    <dbReference type="InParanoid" id="W5M3D2"/>
    <dbReference type="OMA" id="LTFYTCR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002412">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF105">
        <property type="entry name" value="SODIUM BICARBONATE COTRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="713"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="743"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="841"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="879"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="927"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="967"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1053"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1104"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="12">
        <location>
            <begin position="115"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="12">
        <location>
            <begin position="428"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="11">
        <location>
            <begin position="597"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="380"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="552"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1274" mass="141501" checksum="AE09DFF307FBC276" modified="2014-04-16" version="1">RDASSNEQPVFPVSLIQGHDEEAVVDLGKTSSTINTNFEKEELESHRAVYIGVHVPLGRQSRRRHRHRGHRHHRRRKDRGSDREDGRESPTYDTPSQRVQFILGTEDDDEEHIPHDLFTELDELSFRDGHATEWKETASCRWLKFEEDVEDGGERWSKPYVATLSLHSLFELRSCIMNGTVLLDMRANTLDEIADMIMDSMVASGQLDESLREKVREAMLKRHHHQNEKKLSNRIPLVRSFADIGKKHSDPHLLEKNGEIFRRGSGDLSDCGTLSTAGGIQKNGVEERLRAKPVHTLASFGLCCMPPPPPPPHCIPSCPPTTYRREEIIKTWHVCETRGPGGFSGLESDAPAWTSGQPELERERVCSVNRTHLKRIGLLASPQSAPGNLEHAKPSESRINGGGGGGGGGGGGGGGGGGGGSRENSTMDFSRVDMNFMKKIPPGAEASNVLVGEVDFLECPIIAFVRLAPAVLLTGLTEVPVPTRFLFLLLGPFGKGPQYHEIGRSIATLMTDEVFHDVAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPTIRIEPPKSVPSQEKRKIPALPNGSAPTPEAAKEEEHHAGPELQRTGRLFGGLILDVKRKAPFYWTDIKDALSLQCLASILFLYCACMSPVITFGGLLGEATKGNISAIESLFGASLTGIAFSVFAGQPLTILGSTGPVLVFEKILFKFCKDYGLSYLSLRTSIGLWTAFLCLVLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLFHLGEMYPVNMHNDLDNLTFYTCQCSEPSNASAETLQHWNKTGFAPNSIPWSELNISMCKSLQGEFVGAACGHHGPYIPDVLFWSVILFFTTFFLSAFLKQFKTKRYFPTKVRATISDFAVFLTIMIMVVVDYLVGIPSPKLHVPDRFEPTSKHRGWLIDPLGDNPWWTLLAAAVPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDLLVVSVMLGICSIMGLPWFVAATVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFVLMGLSVFMTSVLKFIPMPVLYGVFLYMGASSLKGIQFFDRIKLFGMPAKHQPDLIYLRYVPLWKVHIFTIVQLTCLVLLWVIKASAAAVVFPMMVLALVFIRKLLDFCFTKRELSWLDDLMPESKKKKEDDKKKKEKEPPFQEAERMLEVEVSESVQIPFEEGNLLKIPVKTLKCSANSDPSVVNISDEMAKTAVWKAVTMSADNAKVLKRSASNEKMACIKISIEDDQGGKYVDAETSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4N8</accession>
    <name>W5M4N8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.3.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01038905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5M4N8"/>
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        <property type="interactions" value="753"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000003346"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003353.1">
        <property type="protein sequence ID" value="ENSLOCP00000003346.1"/>
        <property type="gene ID" value="ENSLOCG00000002843.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158866"/>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_1"/>
    <dbReference type="InParanoid" id="W5M4N8"/>
    <dbReference type="OMA" id="LYREAPM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
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    <dbReference type="Bgee" id="ENSLOCG00000002843">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
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        <property type="entry name" value="Acyl-CoA_DH_CS"/>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
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        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA_dh_M"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="18">
        <location>
            <begin position="88"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="203"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="16">
        <location>
            <begin position="310"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00012046"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00044204"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00045387"/>
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            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
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        <source>
            <dbReference type="RuleBase" id="RU362125"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF00441"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF02770"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="49989" checksum="44009CDA07C4BC1C" modified="2014-04-16" version="1">MHACALRPRAQDVPFQRPAFRCNTLSHPTRNSALRMPDSGRTYFQARRAVLTLGSWVKMAALLCARKAVLSCRRGGVRLLSQLAELPETHAMLRQTCRDFADRELAPLAAQLDREHRFPAGQVKKLGALGLMAVEAPEHLGGAGLDYLAYALAVEEISRGCASTGVIVSVNNSLYLGPILKFGSEEQKQQWVTPFTSGERVGCFALSEPGNGSDAGAATTVARKEGGQWVLSGTKAWITNCWDASAAVVFATTDKSLKHKGISAFLVPMPHPGVSLGKKEDKLGIRASSTANLILEECRLPLENLLGQPGMGFKIAMQTLDSGRIGIAAQALGIGQAALDCAADYAHKRTAFGAPIAKLQAIQFKLADMAVALESARLLTWRAAMLRDSGKPFTKEAAMAKLAASEAATFISHQAIQVLGGMGYVTDMPAERHYRDARITEIYEGTSEIQRLVIANQVLKEYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4U6</accession>
    <name>W5M4U6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Family with sequence similarity 8 member A1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4U6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003404"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003411.1">
        <property type="protein sequence ID" value="ENSLOCP00000003404.1"/>
        <property type="gene ID" value="ENSLOCG00000002898.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686154"/>
    <dbReference type="KEGG" id="loc:102686154"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007346"/>
    <dbReference type="HOGENOM" id="CLU_053631_1_0_1"/>
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    <dbReference type="OMA" id="DYKSAND"/>
    <dbReference type="OrthoDB" id="10061042at2759"/>
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        <property type="component" value="Linkage group LG11"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="FAM8A1"/>
    </dbReference>
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        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659">
        <property type="entry name" value="AUTOSOMAL HIGHLY CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659:SF5">
        <property type="entry name" value="PROTEIN FAM8A1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
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        <location>
            <begin position="173"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="37437" checksum="C8583863953848CF" modified="2014-04-16" version="1">MADAEERLGERADEDPAAKNSQARRRTGADMNAAGRLGDPQGSEERPAPGGRQTAAEYCGKVQEWMWQYYWGCMNWQSWAAMSAYAFPPCSLSPPANPNGSAPPWGGGGGPSPADVRSWYSSHFPFAAPPYFPVPPAGHAAAGAQAGQLTAGDRPVLQQNGNAPQPGREYTIPSPLHRFIAEMVDFFILFFIKATIVLIIMHFSGMKDISKFAMHFIVEEIDEDTSMEELQKMMVVALIYRVLVCFYEIICIWGAGGATPGKFLLGLRVVTCDTSVLVRPNRVFVVPASNVTLSASTVRALNKNFSIAFFFPAFITLLFFQHNRTVYDIVAGTIVVKRSGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M520</accession>
    <name>W5M520_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022612">
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        <property type="gene ID" value="ENSLOCG00000002957.1"/>
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        <property type="component" value="Linkage group LG14"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="entry name" value="Olfactory receptor C family, g2"/>
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        <property type="entry name" value="Taste 1 receptor member 2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
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        <property type="entry name" value="Peripla_BP_I"/>
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        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCQ19-RELATED"/>
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        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
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        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CASENSINGR"/>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
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    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
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    <feature type="domain" description="Receptor ligand binding region" evidence="11">
        <location>
            <begin position="4"/>
            <end position="224"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="257" mass="28692" checksum="2D219411E585874F" modified="2014-04-16" version="1">METIQQSTARVIVVFSEGAHFFPLAEEIVRQNITDRQWIASEAWVFSTLISRAELMSSFTGTIGIAIRRGEIPGLENFLLGIHPGSGPNTGLVEEFWEINFDCKFEGKTSTNSSLYEVKGKICTGSEELNSRKTAYSDVSKLRASYNVYKAVYALAHSLHNLMSCEPGKGPFQNNSCADITTVQPWQLLHYLARVNFTTHYGDRVSFDENGDALAIYDVINWQRADDGSVKTVTIGLYDESAPAGQQLTLNEDKIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M592</accession>
    <name>W5M592_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Scm polycomb group protein homolog 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCM family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7918.ENSLOCP00000003550"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003557.1">
        <property type="protein sequence ID" value="ENSLOCP00000003550.1"/>
        <property type="gene ID" value="ENSLOCG00000003009.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157999"/>
    <dbReference type="HOGENOM" id="CLU_015000_1_0_1"/>
    <dbReference type="InParanoid" id="W5M592"/>
    <dbReference type="OMA" id="NKEFCSM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003009">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09578">
        <property type="entry name" value="SAM_Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000018">
        <property type="entry name" value="Polycomb protein scmh1 isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000028">
        <property type="entry name" value="polycomb protein SCMH1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mbt"/>
    </dbReference>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047531">
        <property type="entry name" value="SAM_Scm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033763">
        <property type="entry name" value="SCML2_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF68">
        <property type="entry name" value="POLYCOMB PROTEIN SCMH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17208">
        <property type="entry name" value="RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12140">
        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="MBT" evidence="8">
        <location>
            <begin position="127"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="649"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="240"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="347"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="596"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="719" mass="79154" checksum="F39835DE35BC5CC7" modified="2014-04-16" version="1">MRKPGRQRAIEWKDERRHKHARSGRPSRTPPQYQGHFSWEKYLKETGAVAAPAHCFRQISNEMPKIEENNGMTLVGISPFGATSISSASGGQIERCRADRWRPRGWPVLKPPGCVFVCPAGFRLNASSWPMFLLKTLNGAEMAPGRIFHKQEPPAPEQNYFKVGMKLEAVDRKNPHFICPATVGALRGVEVLVTFDGWRGAFDYYCRYDSRDIFPVGWCSLTGDNLQPPGTKAVLPKSLSAAPEGSLEGGGMHPAEGKAAVGVRSRRPGRRKTRLPAAWGQRGVESQSPAGTPKSLDPIKIPKKRGPKPGSRVGWVKRAAWLEGTMTGRPLIRPRGRLPANWGQRGVTAGVQHNPAGSLKALEPIKIPKKRGPKPGSKRKPRLIPNPVPTSPTSSTPEPDTSTVPQDNATIPTAALQAPTVCVYLNKYGKVGPHLDQRRVRQLPDHFGPGRASSVLQQCVQACIDCAHNQSSVFSCLKPGHGGEVISAFFEQEQHTLTLPAVNSVTYVLRFLEKLCHNLHSDNLFGNQPFTMSPLHYDSPVYLADRRSFVEGRGGKRYPQESPPYSISLSAKLVKNHHHPSEDPFFLENGVSGGVDGLDPSLTPGTPTSPLRGSRERTSPGCKPHRLNSGGSERFLGSRDSPRSNGQDPSLWTVEDVMQFVRDTDPLLGPHADLFRKHEIDGKALMLLRSDMMMKYMGLKLGPALKLTFHIDRLKQGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5M0</accession>
    <name>W5M5M0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5M0"/>
    <dbReference type="FunCoup" id="W5M5M0">
        <property type="interactions" value="1440"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003678"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003685.1">
        <property type="protein sequence ID" value="ENSLOCP00000003678.1"/>
        <property type="gene ID" value="ENSLOCG00000003122.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010157"/>
    <dbReference type="HOGENOM" id="CLU_059606_1_0_1"/>
    <dbReference type="InParanoid" id="W5M5M0"/>
    <dbReference type="OMA" id="RICLAVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003122">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF4">
        <property type="entry name" value="PROTEIN YIPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="98"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="304" mass="33759" checksum="2DAA0A96BD749089" modified="2014-04-16" version="1">MTSGITGPEFEDAENLLAANRDATTISIGDPNDKPSNQRRSAGKTPRQEEDLLGNEDSDKTELLAGQKKSAPFWTFEYYQTFFDVDTDQVLNRIKGSVLPLPGKNFVRLYIRSNPDLYGPFWICATLVFAIAISGNISSFLVRHGDPHHHYVPEFRKVTIAATAIYSYAWLVPLGLWGFLMWRNSKVMSIVSYSFLEIVCVYGYSLFIYIPAAVLWVIPNETVRWVSSVEPLGSAGVSGVPCHLSCVSATAKTQIKQPRHLNLSELRLLVFSFPQQYFFDSQEVGLGNAGPTASNQTVAAVKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5Q1</accession>
    <name>W5M5Q1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 93</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC93 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011561">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5Q1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003709"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003716.1">
        <property type="protein sequence ID" value="ENSLOCP00000003709.1"/>
        <property type="gene ID" value="ENSLOCG00000003144.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694102"/>
    <dbReference type="KEGG" id="loc:102694102"/>
    <dbReference type="CTD" id="54520"/>
    <dbReference type="eggNOG" id="KOG2701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011294"/>
    <dbReference type="InParanoid" id="W5M5Q1"/>
    <dbReference type="OMA" id="YERQEAP"/>
    <dbReference type="OrthoDB" id="16092at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003144">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039116">
        <property type="entry name" value="CCDC93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019159">
        <property type="entry name" value="CCDC93_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048747">
        <property type="entry name" value="CCDC93_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441">
        <property type="entry name" value="FIDIPIDINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09762">
        <property type="entry name" value="CCDC93_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21673">
        <property type="entry name" value="CCDC93_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC93 N-terminal" evidence="7">
        <location>
            <begin position="35"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC93 coiled-coil" evidence="6">
        <location>
            <begin position="188"/>
            <end position="629"/>
        </location>
    </feature>
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        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="330"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="564"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="636" mass="74017" checksum="3445E34CCE60A097" modified="2014-04-16" version="1">MAATSVFQRVRTGSKLAQYDQDGNIIQVETREDEEQNIKLLEILELLVAAGYFRARIKGLSPFDKVVGGMTWCITTCNFDIDVDLLFQENSTIGQKIALTEKIVSALPKMKCPHRLEPHQIQGLDFIHIFPVVQWLVKRAIETREEMGDYIRSYSVSQFQKTHSFPEDDDFMLRKEKAIKTITDVSEVYKPQRKYKRQDDAGKLEDEESRVHSTLLEYGRLHDFVMRYGFSKHSRQDKAEDKRAPLTQGLPPGMTDVSEEEDLQAAEELRIKTLMTGMSAMVTEEGKLTASTVGQIVGLQSEEIKQMASEYAEKQSELSSEDRPERFGPVQQHRRLVASLNKQIQQKTKQLEELQAKHAEIQEGCEEAKNRLMEASSLTEKLESKLSALQEVESQADSSVLQKLRALVSMNENLKNQEQEFRTHCREEMARLQQNIENLKIESGDENDEEKERIQLVEKQYNTDREKLQKIRLLLARRNREIAILQRKIDEVPSRAELTQYQKRFIELYGQVAATHKETKQFFTLYNTLDDKKVFLEKEVNLLNSIHDNFQQAMASSGAKEQFLRQMEQIVEGIKQNKIKMEKKKQENKMRRDQLNDEYLELLEKQRLYFKTVKDFKEECRKNEMLLSKLRAKGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7Z5</accession>
    <name>W5M7Z5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Epidermal growth factor receptor pathway substrate 15 like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <location evidence="11">Coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7Z5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004503"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004511.1">
        <property type="protein sequence ID" value="ENSLOCP00000004503.1"/>
        <property type="gene ID" value="ENSLOCG00000003755.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155438"/>
    <dbReference type="HOGENOM" id="CLU_007270_1_1_1"/>
    <dbReference type="InParanoid" id="W5M7Z5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003755">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000026">
        <property type="entry name" value="Epidermal growth factor receptor pathway substrate 15-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000074">
        <property type="entry name" value="epidermal growth factor receptor substrate 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF69">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0168">Coated pit</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="14">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="14">
        <location>
            <begin position="127"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="14">
        <location>
            <begin position="285"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="232"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="593"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="778"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="396"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="891"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="794"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="818"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="854"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="925" mass="101013" checksum="28FA8E5B9995ED8D" modified="2014-04-16" version="1">ILACCFSLFQLSSGNPVYENYYRQVDPGNTGRVGPTEAALFLKKSGLPDITLGKIWDLADPDGKGFLDKQGFYFALRLVACAQNGHDVTLSSLNLTVPPPKFKDTSSPLLSTSAPTGDSHWAVRPEEKGKFDGIFESLAPVNGLLSGEKVKPVLINSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSVLPTSLIPPSKRKKPASALPGSVPVLPGAVPVLPASPPPPKDSLRSTPSHGSMNSLNSAGSLSPKHTLKSAQPSANWIVPVADRSRYDEIFLKTDADLDGFVNGHEVKEIFMQSGLPQNILAHIWALADTRQMGKLTREQFSLAMHFIQQKVSKGIEPPQVLTPDMIPPSERGTPVSDSTSSVGSGEFTGIKELDDISQEIAQLQREKYVLEQDIRDKEDAIRQKTNEVQEMQNDLDRETCSLQKLESQKQDAQDRLEEMDQQKAKLEDMLNDVRQKCQEETQMISSLQTQIHSQESDLKSQEEELSRAKTDLNRLQQEETQLEQSIQAGKVQLETIIKSLKSTQEEINQARSKLSQIQDSQQEMAKSIEQYNNALNGTHGGSMTNLADMSEGFPEKENGGFGAMEDPFKAKTSAFNSNAQELQADPFQSEDPFKTDPFKGADPFKGSDPFLNDPFAKQPPVATDPFGGDPFKESDPFKSTSSDDFFKKTAKSDPFTTSDPFSKSATLPSKPGLFASGDPFASTSPKPKGSDLFGTLDPFGSSSFGSSGGFADFSQMSKANPLRVNKMCFNGGGLAQGFSDDPFRGKQDTPALPPKKNVPPRPKPPSGQSTPVNLPGSGDSSKSSDPFQPFGADASDPFQSKKGFGDPFSGKDPFAPSSSSKASKDSALGFADFSSFGNEAQQLEWAKRESERAERDRLERLRRQEQEDLELAIALSKAEMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8F3</accession>
    <name>W5M8F3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003193">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003195">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004661"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004669.1">
        <property type="protein sequence ID" value="ENSLOCP00000004661.1"/>
        <property type="gene ID" value="ENSLOCG00000003906.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160318"/>
    <dbReference type="InParanoid" id="W5M8F3"/>
    <dbReference type="OMA" id="MFERREA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003906">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF69">
        <property type="entry name" value="MYOSIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="18">
        <location>
            <begin position="33"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="17">
        <location>
            <begin position="86"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1544"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1805"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="844"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1202"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1920" mass="220267" checksum="56AC3265CB5A5ECE" modified="2014-04-16" version="1">VTMSTDVAQLGEAAPFLRRSAGELAGMQMLPSDGKKRCWIPDDREVYIEAEIKETLGDKVAVEAADGRTLLVKEGDVQQMNPPKFDMMEDMAMLTHLNEASVLHNLRRRYANWMIYTYSGLFCVTMNPYKWLPVYKVEVIAVYKGKRRSESPPHIFAIADNAYHDMLRNRENQSMLITGESGAGKTVNTKRVIQYFATVAAAGDAGGKKNKSFNKGSLEDQIIQANPALEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLSSADIEIYLLEKSRVIFQQPGERDYHIFYQILSRKKPELQEMLLVTSNPYDFHFCSQGVVTVDNLDDGEELLATDEAMDILGFSPDEKYGAYKLTGAIMHFGNMKFKQKPREEQAEADGTENMDKAAYLMGISSSDLVKGLLHPRVKVGNEYVTKGQNVEQVNYAVGALAKAVYDRMFKWLVTRINRTLDTKLPRQFFIGVLDIAGFEIFDFNSFEQLCINYTNEKLQQYFNHHMFVLEQEEYKKEGIDWVFIDFGLDLQACIDLIEKPMGILSILEEECMFPKATDMTFKAKLYDNHVGKSSNFQKPKPDKRRKYEAHFELVHYAGVVPYNIIGWLEKNKDPLNETVVGLFQKSSNKLLASLFENYVSSDRDQSSEKKRKKGASFQTVSSLHKENLNKLMTNLRSTAPHFVRCIIPNETKTPGVMDPFLVLHQLRCNGVLEGIRICRKGFPNRILYADFKQRYRILNPNHIPEDKFVDGRKAAERLLGSLDVDHTQYRFGSTKVFFKAGLLGHLEEMRDKRLAKVLTMIQSRARGKLMRMEYQKMFERREAVLVIQWNIRTFMAVKNWSWIKLFFKIKPLLRTAEAEKEISTMKEEHQRLKETLEKCETRRRELEEKQVTLIQEKNDLLLQLQAEQDTLADAGERCDLLIKSKIQLEARLKELAERLEEEEEAALELANRKRKLEDECSELKKDIDDLEITLAKAEKEKHAAENKVKNLTEELSALEETVLRMGREKKALQEGHQQTLEELQAEEEKVLSLTKARTKLEQQLDDLESALDQEKKTRVDLERMKRRLEGDLKLTHETITDLGNEKLQLEEKLKKNESEMSNLSSKIEEEQAMAGQQQKTIKELQARIEELEEELESERAARARAEKQRAELARELEELGERLEEAGGAGSAQAELSKRREAECLRLRRDLEEATLRHEASAAALRRRHADSAAELGEQIDGLQRVKQRLDKEKSELRLEADDLAGSLAHVTKAKATSEKLCRTYEDQLNEAKSRIEELSRQVADLSTQRSRLLTENGEMSRQLEDREAQIGQLTKGRSIFTQQVEDLRRQLEEEAKARSALTHGLQAARHDRDLLQEQYEEELEARAELQRLLSKGNAEVAQWRDKYETDAIQRTEELEEGGSAPCPCRRKLAARLQEAEESVGAAHGRAAALEKARGRLQGELEDLLLQLERAGAAAAALDKRQRGFDRLLAEGKQRHEESQAELEASQKEARALSAELFKMKHAYEEALVGLEAARRENKNLQEEIADLTDQIGERTKSIQEVEKMKKQLEQEKAETQTALEEAEAASEHEESKTLRVQLELSQVKADFERKVAEKEEEIENYRKSYQRAAETLQAALDAEVKSRTEAARVKKKMEGDLNEMEIQLNHAHRQAAEAQKSTRALQLQSKELQVQVDELSQERGELREQAGLAERRSALLQAELXLFSSQNTSLLNQKKKLDSDIIQMQQEVDKAIEESKNAEEKAKRAINDAAVISEELKKEQHTSAHLERMKKNMEQTIKDLQMRLDEAEQVALKGGKKQIHKLETRVRELESELESEQRRHSEAQKGLRKQERRVKELTFQTEEDRKNLARMQELVDKLQLKVKSYKRQAEESEQQATASLVKYRKLQHELDDAEERAEVAESQVNKLRTKSKDLGAKLLVVAIIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8P5</accession>
    <name>W5M8P5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ArfGAP with GTPase domain, ankyrin repeat and PH domain 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the centaurin gamma-like family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004753"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004761.1">
        <property type="protein sequence ID" value="ENSLOCP00000004753.1"/>
        <property type="gene ID" value="ENSLOCG00000003977.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0705">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158956"/>
    <dbReference type="HOGENOM" id="CLU_007326_5_0_1"/>
    <dbReference type="InParanoid" id="W5M8P5"/>
    <dbReference type="OMA" id="PAKRKMW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003977">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08836">
        <property type="entry name" value="ArfGap_AGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04103">
        <property type="entry name" value="Centaurin_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01250">
        <property type="entry name" value="PH_AGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000001">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000109">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000545">
        <property type="entry name" value="arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000298">
        <property type="entry name" value="ArfGAP with GTPase domain, ankyrin repeat and PH domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051282">
        <property type="entry name" value="Arf-GAP_GTPase_ANK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45819:SF3">
        <property type="entry name" value="ARF-GAP WITH GTPASE, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45819">
        <property type="entry name" value="CENTAURIN-GAMMA-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="656"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="11">
        <location>
            <begin position="911"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="29"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="555"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="716"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="586"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="756"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1144" mass="122634" checksum="A55361DE3CADD78F" modified="2014-04-16" version="1">MSRAATLQRRTTYLISLTLVKVEAVGEDGAPAAVPAPEGRSPAGGAPETERKPPHAQPEEEEEETEIEEDDALKGRGDRTPSGSGGSKATERARPRVWGPKERPPLVPAKEGKRETPEGDRGTGARQEEIPGPGEETPEEPEPPGGAAKELKPWKSVDVVEGPRTPSKCQIPVRVGQRPAALLKAQSSAGRRDARDSAAPAAKSLDRKEKAAKASAAVTPGSTRLSWPENEGKPKPRGSGGAAAAAAGARGSPRAEKLKTGSASLPAPISLVPKPPRKGKSRTLDNSDLHSLSQDLRRGKEGAHLPLPLPGPPTSTAGPPSQRTSARDRKMLKFISGIFTKSTPAPASSPGPPLYGSVQRESSEEEAACANSQEWTLSRSIPELRLGVLGSLRSGKSALVNRYITGSYLAAESPEGGRYKKEVLVDGQSHLLLIREESAPDAQFSAWVDAVIFVFSLENESSFQEVYKNYSLLSSYRNTAEIPLIVVGTQDKISSSNPRVIEDARARQLCTDVRRCAYYETCATYGLNVDRVFTEAAQKMALMKKQGALLASCKSLPNSPSHSGGSTPVSGAFPGQSPTCLSRQGPLLSXSSSLPSTPVVSHRELRGGDGGPGCTTPGSLHRAPRRKPSIFTNRRGSDAEKRSVDSKGDIGSGRVIPIKQSILLKRSGNSLNKEWKRKYVTLSNNGVLSYHPNVNDYMQNVHGKEIDLLRTTVKVPGKRPPRAVTTCGPSASHNGLVRDTGGTAVAPSFPSTLLPVEEKSGSVSPRSEKGVQRCPSSLSSKAQSVDSAVEGLASPTSVKDHAPPSPMIDRKKNRRKKSMNMKGDAAMGQAEAKRKMWKLKSFGSLRNIYKTEEENYDFMIVSSSGQTWHFEASTLEERDAWVLAIESQILASLQSCESSKNKTRRDSQSEAVALQAIRNAKGNGFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELNLVLTSIGNAMANSIWESNTHGRQKPTPDATREERESWIRAKYEQRLFVAPLPPAEEGVGLAARLIAAVMERDLPGLLLLLAHSTKEEINAPLAAPPAPPPAPPRSALHAACQLPDIVMTQLLVWYGSDVKARDAQGQTALALARRAGSQECVDILLQHGCPNEMPPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA40</accession>
    <name>W5MA40_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011356">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA40"/>
    <dbReference type="FunCoup" id="W5MA40">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005257.1">
        <property type="protein sequence ID" value="ENSLOCP00000005249.1"/>
        <property type="gene ID" value="ENSLOCG00000004395.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2NU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165690"/>
    <dbReference type="HOGENOM" id="CLU_308577_0_0_1"/>
    <dbReference type="InParanoid" id="W5MA40"/>
    <dbReference type="OMA" id="TEVRMSD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004395">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550:SF7">
        <property type="entry name" value="RNA-BINDING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="761"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="717"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="975" mass="107316" checksum="5975F80208B74B6C" modified="2014-04-16" version="1">MAFYQRVWSAPAVAWTPSPPLSFRAPQLPVAALAHSPCQIILSPATVDVVIAPGSAHRNPPPLCRNSFRELAALPSTDEEATIFHLNRAVFDLVTAEGGLELTDSKLLGWYLSLPVKDRQFIQGEGGLLRFLQRHPALEVSRNCVYTKTLPQGFVIKQPGRLIFHRSRRPTFYCMPHCRKCGASSAPDTEECRNCSAHQARSVQCLSVLFCVEPEKVLLPDCVKQELAIQKPSADPDLSETFESACDSNLVADRDGRVYEPKGARLTPHLPEECVNVIFKEASIEANYSLDMKLESHKIVEGTVEGTSAERGLRYLGETETVDYSVDGLNLEMESLPDYCSLDSTGFDRTAAEPSESPLPSGAVTAEYYMSVQGDHRTTELFAGDWAELWDASSFPSAALWVSEKFDLESSTAACSGCRLQERGGVAGGGGSREPLGQASITVNQMVDAGGDFRASFTSSRATEAKPSMVSTSTNTDGSLQAAGLDRGTQTSKAATVEKHVITELYMEDLDYFTEEFMKLRGAEAELKDLKEKLSRRCCSCGCAQRALSAELRLLALHYEMCQEHCWRLYYTANEGSISGPRAEAPPDSLVCVLQDLQSSYRAMRDSILSGSTLDQLAPLSVSSERILTGQQYTPAQVRSVSLRASGLDYTRHSCSSAECTVPSFHCSVLQIIPVETHDECGPQLITCQVRFCMMHKTISAMTKDLNENWYDAEEDFVSTSQEDDAQHRAGAESPRKTSRQEHRRTHVKGSTIEGENCVGHYLCVRGLSVNVTENEIMTLFEKYRATDVWMTNVGHNSSLRVAFVTLGSASATELAVQETNGKQIQGQSIKVDWIRRPPLVSPDSSKSSQRNRILVHEACANKPASDRAICRTAKGPAGGRRGQLQVRNLDKLKRVERKPTSSGTFIPQHYAEMGSFDRLMAQLSELHPEAGRQRIVGALLELRAQKRGFLSGLPLKTIVQMTSDLLRNPQGARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB74</accession>
    <name>W5MB74_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ecotropic viral integration site 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018339">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB74"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005633"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005641.1">
        <property type="protein sequence ID" value="ENSLOCP00000005633.1"/>
        <property type="gene ID" value="ENSLOCG00000004695.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153846"/>
    <dbReference type="HOGENOM" id="CLU_005350_6_0_1"/>
    <dbReference type="InParanoid" id="W5MB74"/>
    <dbReference type="OMA" id="LWGHIVA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004695">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000002">
        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000002">
        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000003">
        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF11">
        <property type="entry name" value="EVI5-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="161"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="736"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="421"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="559"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="778"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="802" mass="91849" checksum="65517743E15974D9" modified="2014-04-16" version="1">TGLRSAYQRKERKKVATEKVAGKLSSTLSWVKSAVSHGVGQMASQVVSPSASSLHTTSSSTTLSTPALSPSSPAQLSPDELELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLGHLEEDSWILWGRVVNEWEEVRKKKEKQLKELVRKGIPHHFRAIVWQLLCNAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHEFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGVLSLSDCCSCRQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEQLPELHVHFQAQSFHTSMYASSWFLTIFLTSFPLSVATRIFDVFMCEGLEIVFRVGLAILQMNQAELMQLDMEGMLQYFQKVIPHQLDSGPDKVIQAAFQVKYNAKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIDTLEKKGQVTRAQEAEENYLIKRELATVKQQSDEASAQLEQAQSTIRELQQQHRGTFRDAYRLSSLHKLCSVRLSFDKKLKSMCERKQIVIIITDHLYRHLARTAGRWRDSPRKNALNELQEELMSARLREAESQAELKETRQRMMELETQNQISSNHLRRAEQELRSVRERVQLLGTQNKGLLAQLQELRRKQAETECKNKEEVMAVRLREADNIAAMAELQQQIAELEIQKEEGKVQGQLNSSDSSQYVRELKDQIAELEHEIRCLRGQRALSGQPTFDGIHIVNHYMGDDESYHSSDEEGLAGPVGRSAAASASRPFDPRRDVPRIVLRADLEDTDSEDDEEPPSLTVPPESYSTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBA3</accession>
    <name>W5MBA3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell adhesion molecule 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBA3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005662"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005670.1">
        <property type="protein sequence ID" value="ENSLOCP00000005662.1"/>
        <property type="gene ID" value="ENSLOCG00000004714.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R1KU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156093"/>
    <dbReference type="HOGENOM" id="CLU_047574_2_1_1"/>
    <dbReference type="InParanoid" id="W5MBA3"/>
    <dbReference type="OMA" id="HPHRINW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004714">
        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008037">
        <property type="term" value="P:cell recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042271">
        <property type="term" value="P:susceptibility to natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05881">
        <property type="entry name" value="IgV_1_Necl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000184">
        <property type="entry name" value="cell adhesion molecule 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF2">
        <property type="entry name" value="CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="27"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="129"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="229"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="409" mass="44950" checksum="AFA03520116215F5" modified="2014-04-16" version="1">MSKHKVHTVGQLKSNSSTSQGAAQNIPVQGQNLVTDNVSVVEGEVATISCRVKNNDDSVIQLLNPNRQTIYFRDVRPLKDSRFQLVNFSDNELRVSLSNVSLSDEGRYVCQLYTDPPQEAYADLTVLVPPGNPIIESREDIVSEGNETEIICSAIGSKPASKIRWMKGGKELQGESVVEEMEDKMFTVTSTLTLTVSKEDDGVPIICMLDHPAVKDFQAQKYLEVLYKPEVKIIVGFPEGLTREGENLELTCKADGKPKPQRINWMKVDDDVPAHAVITGADLYIENLNKSFNGTYRCIASNSVGESYDDYILYVYDARTTTPLPTTTFTTSTTTTTTTIIPATAEDSRAGEGVPRTVDHAVIGGVVAVVVFAMLCLLIILGRYFARHKGKCCIASACCVTFTDWICQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBU2</accession>
    <name>W5MBU2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="4 14">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="4 14"/>
    <dbReference type="EMBL" id="AHAT01009785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBU2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005851"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005859.1">
        <property type="protein sequence ID" value="ENSLOCP00000005851.1"/>
        <property type="gene ID" value="ENSLOCG00000004867.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017394"/>
    <dbReference type="HOGENOM" id="CLU_065200_0_1_1"/>
    <dbReference type="InParanoid" id="W5MBU2"/>
    <dbReference type="OMA" id="IKREEAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004867">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000007">
        <property type="entry name" value="Protein-S-isoprenylcysteine O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="301" mass="33682" checksum="27A7A67911BF9BC3" modified="2014-04-16" version="1">AAAGSSVSNMAGSKFVLEGRVSLISFVLGLCVVLIPLFTEAGAHVEWAQDVLNGLNGKIAIVVYIAVVNGLLLILYKGPLYKVAVRAGFLGFAFGCGLLMSFSQTSWKYFGWYMCSLSFFHYSEYLVTAIINPHSLSLDSFLINHSLEYTVAAISSWVEFTLERLLVPEMKQLGWLSLLGLLMVLFGECLRKSAMLTAGSNFNHIVQNEKSQSHVLVTTGVYSCFRHPSYVGWFYWSIGTQVMLCNPVCVLGYTLASWRFFRERIEEEEISLIHFFGEDYLEYKRKVPTGLPFIPGIRVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBU9</accession>
    <name>W5MBU9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LisH domain-containing protein ARMC9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216787.1">
        <property type="nucleotide sequence ID" value="XM_015361301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBU9"/>
    <dbReference type="FunCoup" id="W5MBU9">
        <property type="interactions" value="350"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005858"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005866.1">
        <property type="protein sequence ID" value="ENSLOCP00000005858.1"/>
        <property type="gene ID" value="ENSLOCG00000004868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695879"/>
    <dbReference type="KEGG" id="loc:102695879"/>
    <dbReference type="eggNOG" id="ENOG502QQ9W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018026"/>
    <dbReference type="HOGENOM" id="CLU_007962_1_0_1"/>
    <dbReference type="InParanoid" id="W5MBU9"/>
    <dbReference type="OMA" id="QQSDKEF"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004868">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000124">
        <property type="entry name" value="lisH domain-containing protein ARMC9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040369">
        <property type="entry name" value="ARMC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048959">
        <property type="entry name" value="ARMC9_ARM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056327">
        <property type="entry name" value="ARMC9_CTLH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048957">
        <property type="entry name" value="ARMC9_LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881:SF4">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21050">
        <property type="entry name" value="ARMC9_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21051">
        <property type="entry name" value="ARMC9_LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23138">
        <property type="entry name" value="CTLH_Armc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARMC9 CTLH-like" evidence="11">
        <location>
            <begin position="57"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="LisH" evidence="10">
        <location>
            <begin position="462"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="580"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="651"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="747"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="584"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="665"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="703"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="795"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="811"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="828" mass="93081" checksum="5BC16058F05364EF" modified="2014-04-16" version="1">MGDILVYEGELLGMVKEYLRFAEFDETVKLFEKECKTKGKPLPKTTGNVLRDSKTLIIQKEMISSFEDGDLKVFFELWNEHIPAQIRDVDLLAQKLEFYLHIHFAIFPLKHVLGTPDRTDFEERITHFRHYLETRGAALSQTTEFLPFYALPFVPNPKVHPSFKTLFQDAWTPDLKTRLEKFLSVTLKASSTPKLLTLYKDSGQGNKDILQQLVDAERRAATYMKRYNKMQADYHNLIGVTAELVDSLEATVNGKMITPEYLQSICVRLFSNQMRQSVAQSIDFTRPGTGYYSMSPNDNDDGYASSMLRASIPTPKSKDVPLLPSLDYEKLKKDLIYGTDRLKALLLQALRWRLTKSFHGEQRETVLQAYISNDVLDCHSTSQKNVLQLLNSKNEVVRQYLARLINAFASLSEGRMYLSQSPVLLRVLESTLRVEEKDSLTRENVLGALQKLSLKRALQSTMIQDGLIPWLVDELQDTDALSDYTLEYSVALLMNLCLRTQGKRKCAQNAKHVLKVLTDLLGHENHEIRPYVNGALYSILSVSAVREEARAMSLEEILHCFSKEENAEMNRQIEFIIKQLNSEEVSEDGPQSDDEDEEDDEDEDQDAMEADLDKEEVVQPQLRELSGEALLTTEYLGIMTNTVKAKRKSAQAMSQVVDEPLQRPVTPSSHRTTYTPDSRLGSGGRCALSTHRSEVSLPQSQCSSRPPTRSGSRPSSAESLSPILAADADCGRSSQRLGLSGHLEALSLQPLPSRAPEENGLSGSENIPGFTSRPRIPRTPDCGSTSPRKARTPTLAPQFSQSGPQQTSRPSSAGSLGRSRQSSQSYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBV9</accession>
    <name>W5MBV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005868"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005876.1">
        <property type="protein sequence ID" value="ENSLOCP00000005868.1"/>
        <property type="gene ID" value="ENSLOCG00000004880.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162510"/>
    <dbReference type="HOGENOM" id="CLU_001570_22_3_1"/>
    <dbReference type="InParanoid" id="W5MBV9"/>
    <dbReference type="OMA" id="VESSWIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004880">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046222">
        <property type="term" value="P:aflatoxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11026">
        <property type="entry name" value="CYP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000010">
        <property type="entry name" value="cytochrome P450 2W1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF395">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AC, POLYPEPTIDE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="423" mass="48820" checksum="58997A6D5D644F90" modified="2014-04-16" version="1">MGRRKFVVLTGYQTVKEALVDQADDFGGRGQTHTSQLISKEKGIVFGQGESWKTMRRFTLSTLRDFGMGKRTIEDRIMEESKMLIEVFESYKGQPFEPTIHINSAVSNIICTIVFGDRFEYNNPQFLRLQKLVNENVRLIMSPKMQLFNIFPFIGYFIKDFKKVSSNSNETQSFFRKLYKERKNAVDPNDMRSFIDTFIVKQHEEEAKNSHTYFDEDNMIFTSANLFGAGTETTSTTLRWGLLLMMRYPHIQEKVKAEIERVIGRERPPRSEDRKSMPYTDAVLHEVQRFGNVVPMNVLHETTVDTTFRGYKIPKGTSVIPLLTSVLFDKTQWDTPFEFNPGHFLDAQGNFVRKEAFMPFSAGRRVCLGETLAKMELFLFFTALLQKFTFHLPPGIRPEDLNMEPVPGITASPHSYKLCAVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCK8</accession>
    <name>W5MCK8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCK8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006117"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006125.1">
        <property type="protein sequence ID" value="ENSLOCP00000006117.1"/>
        <property type="gene ID" value="ENSLOCG00000005080.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286971"/>
    <dbReference type="HOGENOM" id="CLU_002678_2_2_1"/>
    <dbReference type="InParanoid" id="W5MCK8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005080">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001837">
        <property type="entry name" value="Zgc:110821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001344">
        <property type="entry name" value="Zinc finger protein 16 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000213">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000925">
        <property type="entry name" value="Zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="38123" checksum="C32D710C2C1AB6A2" modified="2014-04-16" version="1">GCAACGRAFSRRSDLGTHERTHTEERLHGCAECGKSFRRAAHLRSHALIHTGELPFPCPDCGKRFRHLTNLRTHRLIHSGEQPYHCPDCGKGFRQRGHLKLHRRVHTGERPHRCPDCGKDFGRLVYLQSHRRVHTGETPYYCGDCGRCFSRPGSLQIHRLVHSQEKRYRCGQCGKDFRHYSGMKAHERVHTEERPYRCGECGKAFRGSGGLQIHKRSHTGLRPYACPQCGKSFKESGVLKNHLRLHSGELPYRCQDCGKRFRQLGSYRTHLRLHSGEKPYACAHCPKRFRHSNSLQAHNKIHTGEAPFPCPDCGQLYKHMRSLVLHRKTHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCM3</accession>
    <name>W5MCM3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">B-cell receptor CD22-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCM3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006132"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006140.1">
        <property type="protein sequence ID" value="ENSLOCP00000006132.1"/>
        <property type="gene ID" value="ENSLOCG00000005091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107080076"/>
    <dbReference type="KEGG" id="loc:107080076"/>
    <dbReference type="eggNOG" id="ENOG502S6I9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222294"/>
    <dbReference type="HOGENOM" id="CLU_074828_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCM3"/>
    <dbReference type="OMA" id="FIFESEM"/>
    <dbReference type="OrthoDB" id="6250964at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005091">
        <property type="expression patterns" value="Expressed in bone element and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865957" evidence="2">
        <location>
            <begin position="24"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="138"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="38305" checksum="8E1280DA1B4F85B9" modified="2014-04-16" version="1">MRVPDSLCLWAATLLILPGGLCGDWSVLYPQTSICAVPGSTVVIPCSYNYPSKDRLKVNTVMWCINDTDCIADSETKHYVYHSQTPNNRLKFSQRIRYLGNKMDNCTLKITDVGISDQETYRFRFETNDPQGRWVGQPGVTLSITELKIQMSPTGKLEEVREGDNLNLSCVPEGCSLTQTEFTWFKNGQPLSSPWATENTLRFNPVSSEHAGTYSCALKGHAKTESVMFALDVRSEFNTLMIAAILGLVLLVSIATLVIYICIRRKKAGQLKGENSRNMKARDVLNNIYDEVLQPNIPESEHMQRTPSEEDEVCYSAVSFPPKTASCQETNDSSVLYSTLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDW7</accession>
    <name>W5MDW7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ceramide transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Collagen type IV alpha-3-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-hexadecanoylsphing-4-enine(in) = N-hexadecanoylsphing-4-enine(out)</text>
            <dbReference type="Rhea" id="RHEA:45720"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014705">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626774.1">
        <property type="nucleotide sequence ID" value="XM_006626711.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220260.1">
        <property type="nucleotide sequence ID" value="XM_015364774.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220270.1">
        <property type="nucleotide sequence ID" value="XM_015364784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDW7"/>
    <dbReference type="FunCoup" id="W5MDW7">
        <property type="interactions" value="1877"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006576"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006584.1">
        <property type="protein sequence ID" value="ENSLOCP00000006576.1"/>
        <property type="gene ID" value="ENSLOCG00000005451.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687266"/>
    <dbReference type="KEGG" id="loc:102687266"/>
    <dbReference type="CTD" id="10087"/>
    <dbReference type="eggNOG" id="KOG1739">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155123"/>
    <dbReference type="HOGENOM" id="CLU_017289_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDW7"/>
    <dbReference type="OMA" id="LETCHRI"/>
    <dbReference type="OrthoDB" id="2344588at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005451">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13283">
        <property type="entry name" value="PH_GPBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08872">
        <property type="entry name" value="START_STARD11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000104">
        <property type="entry name" value="collagen type IV alpha-3-binding protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000003">
        <property type="entry name" value="Collagen type IV alpha-3-binding protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041952">
        <property type="entry name" value="STARD11_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF53">
        <property type="entry name" value="CERAMIDE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="15">
        <location>
            <begin position="415"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="624" mass="70976" checksum="E4AA2CEFB3BFE087" modified="2014-04-16" version="1">MSDNQSWNSSGSEEDLETESGPPVELGGILSKWTNYIHGWQDRWVVLKNNTLSYYKSEDEREYGCRGSLCLSKAVITPHEFDECRFDISVNDSVWYLRAQDPEHRHQWMDSIEQHKAESGYGSESSLRRHGSMLSLTSAASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRPDGEFLHNNNGSKEKLFPCVNPKGINGIDFKGEAITFKATTAGILATLSHCIDLMVKREDSWQKRLDKEMDKRRRVEDAYKTAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKTRIHRPTAVPSDDAYSAVGKHRFGQKPYSRSSSMSSIDLVSASDDVHRFSTQVEDMVQNHITYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCHYFWNVEVRNDWETTIENFNVVETLSDNAIIVYQTHKRVWPASQRDVLYLSAIRKIIANNENDPDTWIVCNFSVDHNNVPLTNRCVRAKINIAMICQTLVSPPEGDKEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEM4</accession>
    <name>W5MEM4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Adenylate kinase 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024882">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEM4"/>
    <dbReference type="FunCoup" id="W5MEM4">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006833"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006841.1">
        <property type="protein sequence ID" value="ENSLOCP00000006833.1"/>
        <property type="gene ID" value="ENSLOCG00000005659.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693456"/>
    <dbReference type="KEGG" id="loc:102693456"/>
    <dbReference type="CTD" id="158067"/>
    <dbReference type="eggNOG" id="KOG3078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164784"/>
    <dbReference type="HOGENOM" id="CLU_044905_0_0_1"/>
    <dbReference type="InParanoid" id="W5MEM4"/>
    <dbReference type="OMA" id="DCIRRGW"/>
    <dbReference type="OrthoDB" id="522106at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005659">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd22979">
        <property type="entry name" value="DD_AK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="487" mass="54687" checksum="A5DBC63EAA61CC77" modified="2014-04-16" version="1">MDATTKPLRIPPEMGIYAEKHEIFDLLQSMLTRLLVDEPEDPIQFLIDLLKRDGLQVPRVFLLGPPASGKRTIAKMLCEHTHATQINASSLLKEESDLVKRALQYQEKQQEIPSELWIKLVQQRLSKMDCIKRGWVFEGLPRTREQALLLQEAGIAPEHVVFLDAPDTVLIERNLGKRIDPFTGDVYHATFSWPSDDVVARRLQEAPGISEEETAAQLLQFHRESHAVQRTYQQQLRVINADQPSADVFSQTLTFALSRPRSAAPHTPRVVLLGSPGSGKSLQAAIIAQKYNVVNICCGQVLKSAVADETSLGELIKTYLESGQQVPDSVVVKILTERLSRLDCTTRGWILHGFPRDLEQAEKLHEAGFVPNRVFFLEIPNDVAIERITLRAVDPVTGERYHSVYKAPPGPPVQSRLQWNPRDSEEGLLQSLAECRAHAPDLQDFYPHTVHINADQDPHAVFESLESRLVNRLPRSSFGSSLSALTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI24</accession>
    <name>W5MI24_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Chromatin assembly factor 1, subunit A (p150)</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CHAF1A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI24"/>
    <dbReference type="FunCoup" id="W5MI24">
        <property type="interactions" value="1209"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008033"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008043.1">
        <property type="protein sequence ID" value="ENSLOCP00000008033.1"/>
        <property type="gene ID" value="ENSLOCG00000006653.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4364">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000034888"/>
    <dbReference type="HOGENOM" id="CLU_014846_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI24"/>
    <dbReference type="OMA" id="DPWAQDK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006653">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021644">
        <property type="entry name" value="CAF-1_p150_acidic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029105">
        <property type="entry name" value="CAF1-p150_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029091">
        <property type="entry name" value="CAF1_p150_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272:SF0">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A CAF-1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15539">
        <property type="entry name" value="CAF1-p150_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15557">
        <property type="entry name" value="CAF1-p150_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11600">
        <property type="entry name" value="CAF1A_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 subunit p150 N-terminal" evidence="13">
        <location>
            <begin position="24"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 p150 subunit acidic region" evidence="10">
        <location>
            <begin position="266"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="11">
        <location>
            <begin position="476"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit p150 C-terminal" evidence="12">
        <location>
            <begin position="585"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="517"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="683"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="754"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="809"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="261"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="763"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="822"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="871" mass="97180" checksum="0735980FD19CD58A" modified="2014-04-16" version="1">CRGAMLAAEELSSEGPLAQTPRRRGMEGKTKSSSAAKKLVQARLPFKRLNPVPKQNGEPKRTRGPPAPPSPEGSASDTENEQDRSPLPVPPRSAILVNGRGPLDGFMSRARRVTAPPEPCVTIDLTEDSNSAPAPDTAPSPALAQDTAPAGGSITPTSMPEPHSTTASMETGPLDSQTGACTQDPDHSEHPQALEAEAEVEGEEESVSGPENESLLSPSSSSSLSAVESSPEPAKGGTPLRAEAPGSTATEDKKVKRRSLKQGEEQEQRRRQREEQRAEREQQRSEARAARERAKEEARRRREEKERDKREKKEKEEKEKAEKLRAKEEQRKVKLEAKLEEKRKKEEEKRLKEEKDRIKAEKAEITRFLQKPKTLQVPKTLAAACGKFAPFEIKENMVLAPLSRVQCEEEALEQLDRYLAEPDHSLDCRRHWLSCKHRRSGPTWPRPTEDVNSCVLVVEVPKPDGVPDRRQWSRMKLLQFHENYRPAYWGTWSKRSRLISPRCPLQQDKELLDYDVDSDEEWEEEEPGESLSHSEGDEEEEGGDEDDDDDGFFVPHGYLSEGEGASEEEERADPENQKVRQRLKAREWDELMSKGKKFHVLEPVVLGCVWEAQGEPDRPENRMLRLHAVCLLDVPPGDEAGSPESSARERRDEQILSQLLPLLHGNTHGSKVIIMEFQECCRRRPPPQPTSPEPGDDGSVPTRYRLKRIISENAVYEKRPAWRRCCWYVHQEVLQRFGQEGLPVPCQWSYLTQGPHSAREESNGTPSNSSVSPPTPVSTAPPLMSSSAKRKSAGSMSITRFMKRCGDAEQGEAMETDGFQADTEEDEDDADCVIVEAHTGSVGDGAQQESTEHMEVTTSDLPAAAATPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIB0</accession>
    <name>W5MIB0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 2 member 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640469.1">
        <property type="nucleotide sequence ID" value="XM_006640406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIB0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008119"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008129.1">
        <property type="protein sequence ID" value="ENSLOCP00000008119.1"/>
        <property type="gene ID" value="ENSLOCG00000006718.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691706"/>
    <dbReference type="KEGG" id="loc:102691706"/>
    <dbReference type="CTD" id="560358"/>
    <dbReference type="eggNOG" id="KOG0569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161061"/>
    <dbReference type="HOGENOM" id="CLU_001265_30_11_1"/>
    <dbReference type="InParanoid" id="W5MIB0"/>
    <dbReference type="OMA" id="NVTAQWL"/>
    <dbReference type="OrthoDB" id="8120565at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006718">
        <property type="expression patterns" value="Expressed in mesonephros and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17432">
        <property type="entry name" value="MFS_GLUT_Class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000029">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF22">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="27"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="505" mass="55586" checksum="598718B47B32EBF6" modified="2014-04-16" version="1">MSESENDLEHLEASEKKPYAWTLILTVCSAAIGGTLQYGYNISIINAPTAHVQRFVNETVLERWGRQLEGYQITLIWSFIVSMFSLGGLVGALLAGPMAIHFGRKKSLLLNNLFVFVSAALALASRAARSFEMIMLARILVGINAGVSMNIQPMYFGESAPKHLRGAVALSSASFTAFGIVLGQVIGLREILGGESHWPFLLATNAIPGLIQLLTLPWFPESPRYLLIDKGDKEACTEALRRLRGCGDLSREMDEMLQEKAAAMGQRAKGPWALIQDRGVRWQLITVVVASSAMQLCGNDSMYFYAYYVFQEAGIQAEKIQYAAIGTGSCEFIAAVTCNLLIERLGRKVLIMGGYLLMACWAVVFMVTLALQEKVSWMPYLGMTCIFAFILSFGMGPAGVTGILPTEIFEQTARPAAYMIAGSLMWLNLFLMGLLFPFIVSGLGTFCFVPFCSVCLLSALFIALFLPETKGKSTLEITKEFNRRNFKGTEQQQYQLGEVLRSTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLE2</accession>
    <name>W5MLE2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Core component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol III active site to constantly monitor transcription fidelity, cleaves mis-incorporated 5'-ribonucleotides and restarts the transcription process. Once Pol III reaches the poly(dT) termination signal, can induce Pol III clamp opening and transcription termination. Pol III plays an important role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 14">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637250.1">
        <property type="nucleotide sequence ID" value="XM_006637187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLE2"/>
    <dbReference type="FunCoup" id="W5MLE2">
        <property type="interactions" value="713"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009201"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009212.1">
        <property type="protein sequence ID" value="ENSLOCP00000009201.1"/>
        <property type="gene ID" value="ENSLOCG00000007584.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691556"/>
    <dbReference type="KEGG" id="loc:102691556"/>
    <dbReference type="CTD" id="51728"/>
    <dbReference type="eggNOG" id="KOG2906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075071"/>
    <dbReference type="HOGENOM" id="CLU_093932_3_0_1"/>
    <dbReference type="InParanoid" id="W5MLE2"/>
    <dbReference type="OMA" id="MEFCDEC"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007584">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="108" mass="12515" checksum="E987561F01F3C1E4" modified="2014-04-16" version="1">MLLFCPTCGNVLIVEEGQKCYRFACNTCPYIHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTPETCPKCEHPRAYFMQIQTRSADEPMTTFYKCCNQQCGHRWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLP7</accession>
    <name>W5MLP7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transglutaminase 2, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="3">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020827">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020828">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLP7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009306"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009317.1">
        <property type="protein sequence ID" value="ENSLOCP00000009306.1"/>
        <property type="gene ID" value="ENSLOCG00000007654.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTRA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244866"/>
    <dbReference type="InParanoid" id="W5MLP7"/>
    <dbReference type="OMA" id="STWNFHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007654">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013808">
        <property type="entry name" value="Transglutaminase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF73">
        <property type="entry name" value="NOVEL TRANSGLUTAMINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00547">
        <property type="entry name" value="TRANSGLUTAMINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="4">
        <location>
            <begin position="266"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="694" mass="77057" checksum="4182C4E4C4323544" modified="2025-10-08" version="2">MCRCAGLLEAVDLQCWTNNQQHRSQEMDTERLILRRGQPFSLTAHSAGPPPQPPARRLAIVLHLGRDGDTVIKVSDSRGPAGQWWFSVQPAQGELLLTVHSPPDAPVGYYRMNIVVLDSDGLILQQMPAGSFFLLFNPWCKDDAVHLSGEEQLQEYIMNESGLLFQGSWDQINTLPWDFGQFERDVVDICFQILDNSPAALRNPRADALSRSDPVYVSRTVTAMVNANDDRGVVSGRWDGDYSDGVAPTRWTGSVAILRRWSRGGAQRVQYGQCWVFSGVACTVLRCLGIPTRCVTNYSSAHDTDGNLTVDQCYNEQLESLPAAGKDMIWNYHCWVESWMARPDLPRDYWGWQVLDPTPQERSDGVFCCGPCPVRAVKEGDVDVKYDAPFVFSEVNADLACWVVRADGGRSRAALQRHSVGRNISTKSIYGEQREDLTHHYKYPEGSAMERQVYAKAGRKLALRGSGAGQLELGIKQPQAVHGSDFDVYVHVRNRGGEDERGQLTVIARAVTYNGIRLGECHRKTTSLALPAGAAHREVLRVRYEQYGERLSEHHLIRVTALVEPHGRPDVTLHEINIPLSVPRLVVKVIGEAVVSQPLKAQITFVNPLPVTLRGGVFIFSFAKTFSPTQSVLGEEIGPGQTLTVQVSFKPSKPGLRTLLVDFDTDKLKDVKGLAKVIVRPKAQNASPTETEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMQ8</accession>
    <name>W5MMQ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMQ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009667"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009678.1">
        <property type="protein sequence ID" value="ENSLOCP00000009667.1"/>
        <property type="gene ID" value="ENSLOCG00000007962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS9E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="HOGENOM" id="CLU_002274_2_1_1"/>
    <dbReference type="InParanoid" id="W5MMQ8"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007962">
        <property type="expression patterns" value="Expressed in heart and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="94"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1084" mass="121668" checksum="EC8AACF9C88FD1D8" modified="2014-04-16" version="1">MSISHLKSIHQEELQRRYSTLFEYASRLGGQRASTNKNFTHPWLTEGVLGPAGHTHEVLEAFHGFNERAEGKTIQLADIFNATPTEQLGTCRPRVVVTRGIAGIGKTACTNWLIHSWVSGSSLTQFDFIFPLPFRELSLLPAGHLYNLTSLLHSYYPYLDCFEQILTSPSRCLFIFDGLDECQFSLDFTSCPEHCSFSEPALPVCLVVNLIRGHLLPCASVWITSRPAALACLPVHCIDRMTEIQGFLDWQKKEFFCRRLGSCGMDIVAQLKKQRTLYNMCYIPAFCWLVAIILERSPVMIEEKCMPQNLTEVYTKFFMLAVVYQHHRGHGSQRSVEDANEVLRSNSATIQNLSRLAYDGLIVQKLVFSRQDLKEYNLERLQLYQGLISEFVAEDNGPFCRKAHSFVHLTVQEYFAALFVLISCSVPNKSSLFSLQIECFGSGCPQALFIAFKKACQEAIKSSSGHLDLFLRFLCGLALECNQRLLQGLLGKVKEKHDVTPQMVKYIHKILETDLSPDRCLNLLYCLKELRDNSLVQKIKDLLATGSLSSCSLSSAEYSALAFVLETSDDNLDVFKLNPYISVPDGLRRLSPVAKLYRRLDFYYDHPFCDNPCTLIICTPSGRDFCLEEMDSGWVEKILQIGKFLNNVEEQKGRLHRCVIYRNPEAFVKVLFNANSNNLRVFQLPSNLIRYIHLDDSVWEKLWTALKSPNCKVKTLSLKNCKLQPRHFKEIGKLLTNSPINELDLNFNYGGDLGVKQLMEGLNSATCSVSKLRIAGNDLSDECCKDLLTFLTSKQSRVHLCLNDNRNIATGLPFLFSGLSHPNCQLKELHLQICGITAKCCEELAANLHSNRSLTKLFLQRNYLEDRGVEQLCSALQQPYSVLHTLQLEGCRLTSRCCGPLAAVLETSRSLSKLSLGWNELGDDGMKVLCRGLQAPGCVLQTLELESCELTAGCCKNLAQVLSSNLSLTTLILTDNDLEDKGVALLSLGLRQPSCALQTLVLQENRLKGACCKDLRQALTSSSSLITLDLSMNLLTDQSVTELQGLAKHCLSLCTIELRLNKFSCQGKRKLKSLECGLKASINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNJ8</accession>
    <name>W5MNJ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">H6 family homeobox 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNJ8"/>
    <dbReference type="FunCoup" id="W5MNJ8">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009957"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009969.1">
        <property type="protein sequence ID" value="ENSLOCP00000009957.1"/>
        <property type="gene ID" value="ENSLOCG00000008201.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158286"/>
    <dbReference type="HOGENOM" id="CLU_064096_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNJ8"/>
    <dbReference type="OMA" id="DKKPACR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008201">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048840">
        <property type="term" value="P:otolith development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048920">
        <property type="term" value="P:posterior lateral line neuromast primordium migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021510">
        <property type="term" value="P:spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="180"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="182"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="110"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="34701" checksum="6E4EC2DCC718E4B6" modified="2014-04-16" version="1">RHSDHELQAAMPETAQENCTPAKESPFSIKNLLNCDNKPSKPKTLLTQTKAALEGGFSLSQVGDISFPRFEIPTQRFALPAHYLERAPAWWYPYTLSSSGHLHRTDVAEKANVRDSSPASGTDRDSPDLVLKSDPDAKEDEASKSAEEIILEESDSEEPKKEDTLDDWKKREDSPDKKPCRKKKTRTVFSRSQVFQLESTFDMKRYLSSSERAGLAASLHLTETQVKIWFQNRRNKWKRQLAAELEAANLSHAAAQRIVRVPILYHENSASETGSPAGNVPASQPLLTFPHPVYYSHPIVTSVPLLRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNL5</accession>
    <name>W5MNL5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DiGeorge syndrome critical region gene 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gonadal family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNL5"/>
    <dbReference type="FunCoup" id="W5MNL5">
        <property type="interactions" value="979"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009974"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009986.1">
        <property type="protein sequence ID" value="ENSLOCP00000009974.1"/>
        <property type="gene ID" value="ENSLOCG00000008215.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017663"/>
    <dbReference type="HOGENOM" id="CLU_096921_1_1_1"/>
    <dbReference type="InParanoid" id="W5MNL5"/>
    <dbReference type="OMA" id="CVLNESI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008215">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010849">
        <property type="entry name" value="Gonadal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054">
        <property type="entry name" value="DIGEORGE SYNDROME CRITICAL REGION 6 DGCR6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054:SF2">
        <property type="entry name" value="PROTEIN DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07324">
        <property type="entry name" value="DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="193" mass="22297" checksum="4B1C97657D8475BD" modified="2014-04-16" version="1">YPGIYDEPADSTRQQERHYHLLSQLQNLVKDLPSSFQQRLSYTMLSDLALALIDGTVFEIVQGLLEIQHLTERNLYNQRQKLHSEHRGLKQELVRKHKEALQSCKSHNLAVLRSSQQAEIEALEQRVKDEQRMMDEKIVAEMDQKVIDQQTTLEKAGVPGFYTTTSPQELTLQMNLLELILKLQQKESQSGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNW4</accession>
    <name>W5MNW4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cysteine and serine rich nuclear protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AXUD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNW4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010073"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010085.1">
        <property type="protein sequence ID" value="ENSLOCP00000010073.1"/>
        <property type="gene ID" value="ENSLOCG00000008295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692766"/>
    <dbReference type="KEGG" id="loc:102692766"/>
    <dbReference type="CTD" id="80034"/>
    <dbReference type="eggNOG" id="KOG3813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183072"/>
    <dbReference type="HOGENOM" id="CLU_034103_1_0_1"/>
    <dbReference type="InParanoid" id="W5MNW4"/>
    <dbReference type="OMA" id="YYQLGGS"/>
    <dbReference type="OrthoDB" id="5946974at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008295">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031972">
        <property type="entry name" value="CSRNP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023260">
        <property type="entry name" value="Cys/Ser-rich_nuc_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580:SF13">
        <property type="entry name" value="CYSTEINE_SERINE-RICH NUCLEAR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580">
        <property type="entry name" value="TGF-BETA INDUCED APOPTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16019">
        <property type="entry name" value="CSRNP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02031">
        <property type="entry name" value="CYSSERRICHNP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cysteine/serine-rich nuclear protein N-terminal" evidence="10">
        <location>
            <begin position="61"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="571"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="599" mass="66845" checksum="175D8957B5435C76" modified="2014-04-16" version="1">MSGILKRKFEEVDGSSPCSSLRESDDEVSSSESGDSSDSVNPSTSSHFTPSSILKREKRMRTRNVHFESVTVYYFSRRQGFTSVPSQGGSTLGMSSRHSSVRQYTLGEFALEQERIHREMLRDHLKEEKLNSIKLKLTKNGTVESDEANVLTVDDISDDDIDLDNTEVDEYFFLQPLTTKKRRALLRASGVKKIDVEEKHELRAIRVSREDCGCDCRIFCDPETCACSMAGIKCQVDRMSFPCGCTKEGCSNTAGRIEFNPIRVRTHFLHTIMKLELEKNREQQQASAANGYHGETNVHSNPLVQAQHSLEYSLADTVPQTAIMHLQAADELEEPIEDEEEEEDEEENEDDEEEEDDGSSLCSGLTDSSTQSLAASESDEEDDEEDKSDDFGEGMTPPVVSRAEVVPLSSVLCYSDGTVVHENHNSTNSYYTSSSVEYYQLENSNTAAGNIAANQGNEAYSEASSYQDRVTNNNGTMSLVPYSMTSEQYTDYSRQQDERYTNHHFAVSNGASSVIVCCTPDQENNVQSNGIYCEQTPGHSQMEFHNYLNNNSQDRYVTNGNCFTVEQPKEVSNGLSEVSPLPDNKKNPTLESVPEVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP89</accession>
    <name>W5MP89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in nonsense-mediated mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01028177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MP89"/>
    <dbReference type="FunCoup" id="W5MP89">
        <property type="interactions" value="1723"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010198"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010211.1">
        <property type="protein sequence ID" value="ENSLOCP00000010198.1"/>
        <property type="gene ID" value="ENSLOCG00000008380.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154566"/>
    <dbReference type="InParanoid" id="W5MP89"/>
    <dbReference type="OMA" id="CLMSISR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008380">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000017">
        <property type="entry name" value="protein SMG5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF7">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="9">
        <location>
            <begin position="76"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="8">
        <location>
            <begin position="194"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="664"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="530"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="560"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1089" mass="122010" checksum="124EA6DC6EBA7BD7" modified="2014-04-16" version="1">MSGSPQESEPEAKILHTKRLYRAVVECVHKLDIIVHSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKGEELLWRKVYYEVIQVIKTNKKHIHSRSTLECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATPKEMEWAQNACHRCLVYLGDLARYQNELAGVEAETLAERFYQQALSVTPNVGMPFNQLGTLAGSKYYNVEATYYYLRCIQSEMPFDGAYGNLKRLFEKAAKMYHQVKRQEMKKLSPARQRSKDIKRLLVSFMYLQSLLQPKNSLLESELTSLCQSVLEDFNLVLFYQPGAPHCSPTAGEEEEDHDARCSFLPDTLVFRMGVTCLMVVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGDSQVPPLHTDHTDDPEVKEAAGGLTSEEGVLQNGTAGEEEEREEGGEAGKRRGRGGRAKEGMTEEEEEEEEKKQQQQKKQKKYSRLSMLRRRRYARKEDESDLSEGFESEEEDEEEEEEERDRETGSSGPPASAPQPGREGKPQPPEGQGGGWESASEEEEEGGTAFDVDTDSDMNSQESRSDLEDMEDAESHGGEEGGGSDPGDPRRSPPDEVAAPPASNGPSLASDPSITSNLQAMSSQLFQSKRCFRLAPTFSNVLLRPQNPSAPPAPAPSQFTPSPAGELPAQTGPGTANGTNDNEGGSDSEGSEVSNHSSRNEKTLPEKLEILTNQGLLPAVKIFVDWLRTNTDIIVMCGQSSQSLWNRLSVLLNLLPDGSKMLEAGLGLSEEVTRLLSECEQPSLAQTLLLPEDLALRHLPALHLAHRRLDFARERPSLGPLQECVVRVCCIRSFGHYLTNLQGNVLHFNPEAGIFTSISQSEQENLVQLAKAQFRMVREQELSHRPAVISQYNSSSQYPTLLNSLFIYVLSKKSVFSQHRGCVSQLEQSCFKRLIIAVFFIDGLDVLKKENAGARDGIRFLESEFRKGNRYIRCQRESGKSFERDKLKRQDIDAWQLYKILDSCRQLTLSQSSAEDDTAGMVTILAGHPLPDPSALSPHMQAAVQAVLAAGIELKSILDFFRQWKEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPC0</accession>
    <name>W5MPC0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Spermatogenesis and oogenesis specific basic helix-loop-helix 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPC0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010229"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010242.1">
        <property type="protein sequence ID" value="ENSLOCP00000010229.1"/>
        <property type="gene ID" value="ENSLOCG00000008416.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107076751"/>
    <dbReference type="eggNOG" id="ENOG502S71C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174871"/>
    <dbReference type="HOGENOM" id="CLU_609640_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPC0"/>
    <dbReference type="OrthoDB" id="9948648at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008416">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18908">
        <property type="entry name" value="bHLH_SOHLH1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039583">
        <property type="entry name" value="TCFL5/SOLH1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15402:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15402">
        <property type="entry name" value="TRANSCRIPTION FACTOR-LIKE 5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="449" mass="50043" checksum="0A1AAA3D264ED13C" modified="2014-04-16" version="1">MKSPLQTATALLDIYSFHFMFVHLSSTQPLSDLKSLQVIRNNQSKRSEKVICIVIIPEIHFEKDLIGSVADFIFTKPLTLHNIEMVLKGCDFMCQRNKKDSNSNPNPGSDVWAVQKQQEPANSQWDLGPSIQKSDALSLEPPDQPDTGEAQSLSSSSSQKTSCTNEAFLHSRKEQKRRLQIKKCCNHLRDLLPFLTKKRVDTASVLEMTVKYIKYLQCRVPEETLSKVIKALEEKIMRQWYKPVKLPTKRKYIKKKNQTSEPALATEKQIDEDLIDNFIAANVLGRCIQPALQKPSNPCVAIYPPVAPVMVSSSSSCTAVSQEGFPTHQPPGMFMASDPSLSYLPSFSSCTNLHNPYTLEQSSGPGVPQSSYWPVEYPLGCYENSGLPFANDSMAVYTPQPSLTESTGPPQPYRFQLLAINSSSQQPAGSTGMSPFQQYCNGATTFQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQG2</accession>
    <name>W5MQG2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Voltage-gated inwardly rectifying potassium channel KCNH2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Ether-a-go-go-related gene potassium channel 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Potassium voltage-gated channel subfamily H member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Voltage-gated potassium channel subunit Kv11.1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Interacts with DNAJB12 and DNAJB14; chaperones DNAJB12 and DNAJB14 promote tetramerization. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B. Forms a stable complex with KCNE1 or KCNE2, and that this heteromultimerization regulates Inward rectifier potassium channel activity. Interacts with CANX. The core-glycosylated, but not the fully glycosylated form interacts with RNF207. Interacts with NDFIP1 and NDFIP2; this interaction decreases the cell membrane expression by targeting KCNH2, through interaction with NEDD4L, for the degradation through the multivesicular bodies (MVBs)-lysosomal pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.1/KCNH2 sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010096">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634281.2">
        <property type="nucleotide sequence ID" value="XM_006634218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQG2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010621"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010636.1">
        <property type="protein sequence ID" value="ENSLOCP00000010621.1"/>
        <property type="gene ID" value="ENSLOCG00000008725.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689405"/>
    <dbReference type="KEGG" id="loc:102689405"/>
    <dbReference type="CTD" id="100463518"/>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159846"/>
    <dbReference type="HOGENOM" id="CLU_005746_2_0_1"/>
    <dbReference type="InParanoid" id="W5MQG2"/>
    <dbReference type="OMA" id="CHNRHAS"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008725">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086013">
        <property type="term" value="P:membrane repolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF506">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="26">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="25">
        <location>
            <begin position="738"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="888"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1168"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="889"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="904"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1170"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1212" mass="136056" checksum="4939868089DDEDFB" modified="2014-04-16" version="1">MPVRRGHVAPQNTFLDTIIRKFEGQSRKFIIANARVENCAIIFCNDGFCGMCGYTRAEIMQKSCTCEFLYGPHTKKMAIAQMAQALLGSEERKVEIAFYRKDGVCFPCQVDVVPVKNEDGAVIMFILNFEVMTDNKLLRSPDQVLNHKVPSSWINAGRPRGFRLRLPLLRSLGTSKQSLSRDDPEAVVVDMSKPSSEPLALGELLSLPEQNCLGGPEMLWPEDQRALLDGGENQPVNHSSPRPQRLNPDASFSNCSLTRSRSRESFYSVRRASSVDDIEAMKTDFDKKLRSRHASTGAMNHIRTNMLNSTSDSDLIRYRTISKIPQITLNFVDFKPEPFIASPSSDKEIIAPCKLKDRTHNVTEKVTQVLSLGADVLPEYKLQAPRIHKWTILHYSPFKAVWDWLILLLVIYTAIFTPYSAAFLLNDQEDAAQNDCAYSCNPLNVVDLIVDIMFIIDIIINFRTTYVNTNDEVVSHPGRIAVHYFKGWFLIDMVAAIPFDLLIFRSGSEETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACIWYAIGKVERHYSKAPRIGWLDSLGEQLGKHFNDSDPGSGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVREFIRFHQIPNPLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLNRSLLQNCKAFKGATKGCLRALAMKFKTTHAPPGDTLVHAGDVLTALYFISRGSIEILRGDVVVAILGKNDIFGEPINLYARPGKSNADVRALTYCDLHKIHREDVLEVLDMYPEFSDHFWSSLEITFNLRDTNMIPGSPSSDDSDCGFNRLRRRKLSFRRRTEKDDEDAGEVKKSQRLSRKGRKHIINNQSEVRKTEWEGQQNSVSSPSHSSGDEGEEPVITVPAPPSAILELSAAQATHMHFGDTPEGDGDPKTGNTCNALSGAFSGVSNIFSFWSDNRGRQYQELPRCSLPSHPPLSAPLHSITRRQRLEVESRLDLLQKQLNRLESRMTSDIGAIMQLLQRQMVLVPPAYSTVSSPPEPALYPCTADRLGQPVMPLEPETLVSLSQITDVQELSEIPPTSCDLPLNALEQKSADPPQGSSAERQCLETVSQDALCTESHNETKDNTRDTEPKRRLSLPEQYSEEPQDSRTPQRHSSDPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS32</accession>
    <name>W5MS32_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636009.1">
        <property type="nucleotide sequence ID" value="XM_006635946.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS32"/>
    <dbReference type="FunCoup" id="W5MS32">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011191"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011207.1">
        <property type="protein sequence ID" value="ENSLOCP00000011191.1"/>
        <property type="gene ID" value="ENSLOCG00000009176.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698561"/>
    <dbReference type="KEGG" id="loc:102698561"/>
    <dbReference type="CTD" id="79075"/>
    <dbReference type="eggNOG" id="KOG0798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017400"/>
    <dbReference type="HOGENOM" id="CLU_034504_1_1_1"/>
    <dbReference type="InParanoid" id="W5MS32"/>
    <dbReference type="OMA" id="DSESWPF"/>
    <dbReference type="OrthoDB" id="5199543at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009176">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="44850" checksum="29384852A8B54949" modified="2014-04-16" version="1">MRTLEEVNATLQIAKLKEEDLQSVVHCFSFAENVSSEDYCLMELDETLCKHIEDGKSLVIRGDKNEHAVLCSEDKTYDLKIADTSNLLLFIPDCKTPDQFPEETGSQKLIQTQISGFSNSYWELRRCRPKLKKLKKLLMEYPYEGPEGEQEREGAKEKYTMEDLQDRIQASTKELFAQLQNIHACNIGGYWRILDSDYEIKLLGHVTQLVDSESWSFSKVPLSVCLEELGPLEPPEMIEHCLNCYGRRYTNEAGEIHFSLNEDKVCRATAQMLLQNAVKFNLSEFQEVWQQSVPEGMLTSLDQLKGLALVDRNSRPETISLLRVEDLPEETQDRFNCLFSLREKWTEADIAPYIKDLCGEKQTTGALLTKYARSSMQNGVKVYNSRRPVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSJ5</accession>
    <name>W5MSJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing CTD phosphatase 1</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nuclear proteasome inhibitor UBLCP1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01027419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSJ5"/>
    <dbReference type="FunCoup" id="W5MSJ5">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011354"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011370.1">
        <property type="protein sequence ID" value="ENSLOCP00000011354.1"/>
        <property type="gene ID" value="ENSLOCG00000009305.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010107"/>
    <dbReference type="HOGENOM" id="CLU_046931_1_0_1"/>
    <dbReference type="InParanoid" id="W5MSJ5"/>
    <dbReference type="OMA" id="TVHTPKY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009305">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090364">
        <property type="term" value="P:regulation of proteasome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01813">
        <property type="entry name" value="Ubl_UBLCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000060">
        <property type="entry name" value="ubiquitin-like domain-containing CTD phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011943">
        <property type="entry name" value="HAD-SF_hydro_IIID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051658">
        <property type="entry name" value="UBLCP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02245">
        <property type="entry name" value="HAD_IIID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="14">
        <location>
            <begin position="133"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="318" mass="36569" checksum="6ADC99CE9ED59715" modified="2014-04-16" version="1">MSVSVIIKWGGQEYSITTLCEEDTVFDLKQSIKSLTGVLPERQKLLGLKVKGKAAEDDIKLGTLKLKPNTKIMMMGSREESLEDVLGPPPENDDVINDFDFEEEVIEVENREENLAKIARRVKDYKVEILNPPREGKRLLVLDVDYTLFGKLLSIHKVLEILRGSHRTERGSSQSPHSSSMIKAVEFMTVLFVLQVLGVTNNPNYKITFMLDSAAMITVHIPKRGVVEVKPLGVIWGKYSEFYNKKNTIMFDDIGRNFLMNPQNGLKVRPFMKAHLNRDKDRELFKLSQYLKEIAKLEDFSDLNHKHWERYLSKKQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTK0</accession>
    <name>W5MTK0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger, AN1-type domain 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01002950">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTK0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011709"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011727.1">
        <property type="protein sequence ID" value="ENSLOCP00000011709.1"/>
        <property type="gene ID" value="ENSLOCG00000009585.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164966"/>
    <dbReference type="HOGENOM" id="CLU_057016_1_0_1"/>
    <dbReference type="InParanoid" id="W5MTK0"/>
    <dbReference type="OMA" id="QQPHIND"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009585">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF26">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="8">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="219" mass="23580" checksum="CBB2C880557E4BBC" modified="2014-04-16" version="1">TDMAQETNQSPVPMLCTTGCGFYGNPRTNGMCSVCYKEYLTRQQSSGRMSPISPIGAGSTSSSPTSEASAIQRIEASLSNSDVSSVSAAEISRGMPTASLPVTQQMTEMSISREDKVISPKAEPAEPVVTQPTASTSHPNAPGSDEAKAPEMPKTKKNRCFMCRKKVGLTGFDCRCGNLFCGLHRYSDKHNCPYDYKAEAAAKIRKENPVVVADKIQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTK2</accession>
    <name>W5MTK2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Peroxisomal bifunctional enzyme</fullName>
            <ecNumber evidence="8">1.1.1.35</ecNumber>
            <ecNumber evidence="7">4.2.1.17</ecNumber>
            <ecNumber evidence="6">5.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Multifunctional enzyme 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(2E)-hexadecenedioyl-CoA + H2O = (3S)-hydroxyhexadecanedioyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:40259"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:77075"/>
            <dbReference type="ChEBI" id="CHEBI:77080"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:40260"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA = (2E)-2-methylbut-2-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57312"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:31121"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3E)-decenoyl-CoA = (2E)-decenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45752"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:84793"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:45753"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45736"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:84790"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:45737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(3E,5Z)-octadienoyl-CoA = (2E,5Z)-octadienoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:49932"/>
            <dbReference type="ChEBI" id="CHEBI:85108"/>
            <dbReference type="ChEBI" id="CHEBI:131990"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:49934"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3E,5Z)-tetradecadienoyl-CoA = (2E,5Z)-tetradecadienoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:47464"/>
            <dbReference type="ChEBI" id="CHEBI:71586"/>
            <dbReference type="ChEBI" id="CHEBI:87701"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:47466"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:31188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31191"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:31193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>(3S)-hydroxyhexadecanedioyl-CoA + NAD(+) = 3-oxohexadecanedioyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40267"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:77080"/>
            <dbReference type="ChEBI" id="CHEBI:77081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:40268"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:31160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3S)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:31165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:30547"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62075"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:30549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3Z)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45748"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:85415"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:45749"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:45229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:22433"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45900"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="ChEBI" id="CHEBI:85489"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:45901"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:20724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:137480"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:20725"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="37">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="30">In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="8"/>
    <dbReference type="EC" id="4.2.1.17" evidence="7"/>
    <dbReference type="EC" id="5.3.3.8" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01016407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTK2"/>
    <dbReference type="FunCoup" id="W5MTK2">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011711"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011729.1">
        <property type="protein sequence ID" value="ENSLOCP00000011711.1"/>
        <property type="gene ID" value="ENSLOCG00000009587.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157516"/>
    <dbReference type="HOGENOM" id="CLU_009834_16_3_1"/>
    <dbReference type="InParanoid" id="W5MTK2"/>
    <dbReference type="OMA" id="YNGAAMG"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009587">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.50:FF:000006">
        <property type="entry name" value="Peroxisomal bifunctional enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000052">
        <property type="entry name" value="Peroxisomal bifunctional enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000296">
        <property type="entry name" value="peroxisomal bifunctional enzyme isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF49">
        <property type="entry name" value="PEROXISOMAL BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="39">
        <location>
            <begin position="300"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="38">
        <location>
            <begin position="476"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="38">
        <location>
            <begin position="617"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00035909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00035959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00036336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00036370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00036656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00036989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00038365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00039632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00042031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00047613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="721" mass="78076" checksum="5324B679E05500C7" modified="2014-04-16" version="1">MAVYTRIPGSVAVITLTNPPVNALSSTVRKAISQTVSRAGADSEVKAVVLCGDNRMFCAGAGADIREFSGTMSGPALVPLIDEIEGGEKPVVAAIEGVALGGGLELALGCHYRIAHSEARVGLPEVTLGLLPAAGGTQRLPRLTGIPAALELITTGRHVSAPEALKLGIVDKVTDGNVREVAVEFARSIAGHPLGPRRVSSWPVHCPPDLGRLLEEALTRIRQSSRGAMAPVACVQALRAAATLSFTQGMEMEKALMATLFNSGQARALQYLHFAQRAVGKWSMPSGACWHNTKPRPFHKAGVIGLGTMGKGIAVSLIRAGISVIAVEMGEKQLEVGRKAVNAMVERNARKLGKAPALALIDFTLNLQDLCDADLVIEAVFEDMNLKKQIFRELSSVCRPSALLCSNTSGLDIDEIASVTCRPELVVGTHFFAPAHVMKLLEVVRGSRTSPVTVATAMQLGKQLDKIGVLVGNCSGFVGNRMLRPYTEQAYFLLEEGARPEQVDQVLEEFGFAMGIFKMSDLSGLDVGWRVRKELGLTGLILPSGAAPRIRSGHRYSPLPDILCEKGRFGQKSGWGWYLYDKPGGRVATPDPWLETFLEEYRNIHGIRARRIDPQEILERCLYSLINEGFCILEEGIAAMPEDIDAIYVFGYSWPRHRGGPMFYASMVGLPRVLEKLEHYHQAHPDVPQLEPSGLLRKLVASGSPPIQQWGQVIKKHRSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTK6</accession>
    <name>W5MTK6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="15">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Probable methyltransferase required to silence rDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
            <location evidence="1 15">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01013731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTK6"/>
    <dbReference type="SMR" id="W5MTK6"/>
    <dbReference type="FunCoup" id="W5MTK6">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011715"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011733.1">
        <property type="protein sequence ID" value="ENSLOCP00000011715.1"/>
        <property type="gene ID" value="ENSLOCG00000009591.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684313"/>
    <dbReference type="KEGG" id="loc:102684313"/>
    <dbReference type="CTD" id="23378"/>
    <dbReference type="eggNOG" id="KOG3045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006189"/>
    <dbReference type="HOGENOM" id="CLU_027694_2_4_1"/>
    <dbReference type="InParanoid" id="W5MTK6"/>
    <dbReference type="OMA" id="DPQAFWV"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009591">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005677">
        <property type="term" value="C:chromatin silencing complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046015">
        <property type="term" value="P:regulation of transcription by glucose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 15" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="49"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="526" mass="59126" checksum="71010289427F5414" modified="2014-04-16" version="1">MFAEEEWNDEPTALVLTQAVFHHRQLSDFKSVTGVGNKKSLLRTLQTLGSVPDWNSPCKEGSSDGETDKAPQAAKKKRVRKNYKTAKTEVKDNEEDEGPVEKKRKAWPQKGGELRAQRDRQGSNKEAEMIRQGECETEQLKKDEGQSKVSRKQWKNKMKNKRKCKNKYRQNGQVSTALVVRKDGSGSLERSTVESKTTHSSLQVSQRQEQNKRGEGPGKKEPKGSAAKKVAFRSVEGSGLKSQPDGEEIETSGLWAISARKRKKDPKQQQAKLQKILKVLESKKTGSSVSQKEEKVADSPVPALDRSSALRARMEQRLESARFRYINEQLYTSTSQEAYRMFQQDRQAFNIYHQGFKAQVERWPNNPVDSIISYIRNKPSSMVVADFGCGDCKIAHSVKNKVHSFDLLPISDLVTVCDMAHVPLPDGSVDIAIFCLSLMGTNIGDFVVEANRVLVKSGILMIAEVASRFENVRSFLSALSTLGFKLATKDTSNCYFYTFELIKTKESEGKSHTAGLELKPCMYKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTY1</accession>
    <name>W5MTY1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dual specificity phosphatase 27</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MTY1"/>
    <dbReference type="FunCoup" id="W5MTY1">
        <property type="interactions" value="677"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011840"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011861.1">
        <property type="protein sequence ID" value="ENSLOCP00000011840.1"/>
        <property type="gene ID" value="ENSLOCG00000009695.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689694"/>
    <dbReference type="KEGG" id="loc:102689694"/>
    <dbReference type="CTD" id="92235"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159723"/>
    <dbReference type="InParanoid" id="W5MTY1"/>
    <dbReference type="OMA" id="LSMQTNH"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009695">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014866">
        <property type="term" value="P:skeletal myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14576">
        <property type="entry name" value="DSP_iDUSP27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF4">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE-INTERACTING-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="133"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="202"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="781"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="868"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="793"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="907"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="922"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1011"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1040"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1146" mass="129287" checksum="DE97C2A9930ED998" modified="2014-04-16" version="1">MASRGDPAGEQVVPEEEDAVSVRSVQARYLRSPSPSRFSIMSDADTESIFMEPIHLSPAVAAKQIINEELKPKELKVEPGVPETMMESAEQLLVEDLYNRVKDMMDDSSPFDTPCVLDIQRALTQDRLEAPTNPVDEVWPNIFIAEKAVAVNKSRLKRLGITHILNTAHGTGVYTGPDFYTGMNIQYMGIEVDDFPEMDISQHFRPAAEFLDEALLTHRGKVLVDSMMGVSRSAVLVAAYMMIFHHMTIMEALMTLRKKRPIYPNESFLRQLRELNETLLEERGGGEDDDSDTLSQSSVIQAKTHSVMAEEEESGSVMGAKAHSIFVEEEDKMSTMSSVLTAAAKSSVVSKRPTLIDEDEEDRLYEEWRKKQGLPPSEKPNPKEVKIPRLPEEKEDEDVDGMIREWQRRNEQYQSEDWWRAQLMSEDEQSLLGGSSFSANGCDDLESVNSVDIRALKERLAASGAGRFRSDSVSTEDSAADIWTKRLREIEEEAAARYGDKGRGATENGTGEKEIDEESLFSETSSLYNFCKKNKEKLTPLERWRIKRIQFGWNKKDAEAEKAEGGSVQGEEEETKVASGEVNLTAYQNWKLKHQKKLGTENKDEIVELSKGEDTASIKKKQRRAEILERSRQTLEESQSMCGWDTESSMSGSIPLSAIWPTMSARSISDDTTSMLSMQSNRSSFSQSRNLQGLPPAPITPLPNIQVGNDDAISLASIQNWIANVVTETIIQKQNELLMQGGLPPSRSPSVMSSGARPGAVGKYMDDDKVSLLSMQSGVSYASSSSRQRELPSTDSHSVLSCSSLSNIKPEGHSSKDKITRTSKPLYSLFADDVDLKKLDTKDKEMKSEMRGKMSAYEKEKIAADNKRSTLFRRKKAKDDKEDEDDTMRASGNYSYTKFDRSDRSDTVPNITGQLSRSVTESADKTSSIDKWLNDIKTPSRYQQKSYGGESSEAKFPRSSYTKEDNIETSYSKSSRGWKEFSDRSLSSLDEVYKSSSRFSSKEPSTASDLHSSDRFGVHSYDDHRRNSTELYDFRRSATTEEATSNIATQKHRSQVTYSSEDDDSEDNHGLEYGAKRRFTQSFSKSEENNRETKRSDSDDEEAPLNTWYRIKSKPKEDEELDDDEVITAWRRQQESKSRTQRRREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU52</accession>
    <name>W5MU52_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU52"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011911"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011932.1">
        <property type="protein sequence ID" value="ENSLOCP00000011911.1"/>
        <property type="gene ID" value="ENSLOCG00000009753.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165361"/>
    <dbReference type="HOGENOM" id="CLU_2372199_0_0_1"/>
    <dbReference type="InParanoid" id="W5MU52"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009753">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033304">
        <property type="entry name" value="DLEC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348:SF1">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="95" mass="10718" checksum="9398609C241CABE7" modified="2014-04-16" version="1">MLQEPSLNTSCGSEPPMNRHRPASEKTQDISHLLASIFKQLYTSEIIGKDKIANLEKSRGGGNSYHEKYVEELQKVSKGLNHHNSKHCMSLITLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVF1</accession>
    <name>W5MVF1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01021370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVF1"/>
    <dbReference type="FunCoup" id="W5MVF1">
        <property type="interactions" value="1717"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012360"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012381.1">
        <property type="protein sequence ID" value="ENSLOCP00000012360.1"/>
        <property type="gene ID" value="ENSLOCG00000010102.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158978"/>
    <dbReference type="HOGENOM" id="CLU_016428_4_2_1"/>
    <dbReference type="InParanoid" id="W5MVF1"/>
    <dbReference type="OMA" id="EHRMVAP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010102">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000002">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000008">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <location>
            <begin position="196"/>
            <end position="315"/>
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            <begin position="409"/>
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            <dbReference type="ARBA" id="ARBA00001663"/>
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            <dbReference type="ARBA" id="ARBA00001946"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03372"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="561" mass="63470" checksum="532EBEE255C3C429" modified="2014-04-16" version="1">ITGMPKEKYDPPDPRRMYTIMSSEDAANGKKSYWAELEISGNVRSLSTALWSLTHLTALHLSDNSLSRIPPDIAKLHNLVYLDLSSNKIRSLPAELGNMVSLRELLLNNNLLRVLPFELGKLFQLQTLGLKGNPLAQEILNLYQEPDGTRRLLNYLLDNLAGTAKRIPTEQPPSRSWIVLQEPDRTRPSALFSVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKSIMQEILSCNADIISLQEVETEQYYNFFLVELKEQGYDGFFSPKSRARTMSESDRKHVDGCAIFFKTEKFSLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKEMIEMSSGKPLHGMEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKATRSLKLSSVSGETNAIPLVLCADLNSLPDSGVVEYLSTGGVDCTHKDFKELRYSDSLTNFNCNGKNGTSNGRITHGFKLKSAYENGLMPYTNYTFDFKGVIDYIFYSKPQLNVLGILGPLDPHWLIENNISGCPHPHIPSDHFSLFAQLELVLPYPPPVNGIHLPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVU2</accession>
    <name>W5MVU2_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Transmembrane protein 64</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000010212.1"/>
    </dbReference>
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    <dbReference type="CTD" id="169200"/>
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        <property type="component" value="Linkage group LG11"/>
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    </dbReference>
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            <begin position="95"/>
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    </feature>
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        </source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="350" mass="37515" checksum="A7040999C8B12539" modified="2014-04-16" version="1">MWSLALTALQVLVKALKHTAGKSQLVVNRWLQRSAPDAMDCEKMEILICSGYDERAAGAKPDLDSQGDGGGEAGVCFFGEFRNPCCLTTFCCKSALLAFILALVCFSSLALVRQYLKDLLVWLEGLDSLVGALLFILGLVVVSFPCGWGYILLNVAAGYLYGFVLGMGLVIVGVLIGTFVAHVVCKKLLTEWVLAKIENNDQLSAVIRVVEGGSGLKIVALARLTPIPFGLQNAVFSITDVSLPNYLVASSVGLLPTQLLNSYLGTTLRTMEDVIAEQSVSGYLVFSLQIMISIGLMFYVVHRAQVELNAAIAACQMELKTSYMNGNPANLSSSSYCSKRTSATGGINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWP9</accession>
    <name>W5MWP9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="3 8">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="3 8"/>
    <dbReference type="EMBL" id="AHAT01003584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWP9"/>
    <dbReference type="FunCoup" id="W5MWP9">
        <property type="interactions" value="1662"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012808"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012833.1">
        <property type="protein sequence ID" value="ENSLOCP00000012808.1"/>
        <property type="gene ID" value="ENSLOCG00000010443.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011864"/>
    <dbReference type="HOGENOM" id="CLU_056472_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWP9"/>
    <dbReference type="OMA" id="PSDCPWG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010443">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="283" mass="31141" checksum="A8FCEEE6DFBA4EB6" modified="2014-04-16" version="1">MALTQVSSNKCSGGFQKVFEHDSTELKCRMKFAIYLPPKAESGKCPVMYWLSGLTCTEQNFITKGGSQQAAADHGVIIVAPDTSPRGCGIEGEDESWDFGTGAGFYVNATEEPWKTNYQMYSYVTEELPRLINANFPADPDRMSISGHSMGGHGALICALKNPGKYKAVSAFAPICNPMQCAWGQKAFRGYLGSESSGWESYDATVLVSSYSGPQLDILIDQGKDDQFLAAGQLLPDNFIAACTEKKIPVVFRLQQQGYDHSYYFVYSFIADHIKHHAKYLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWU7</accession>
    <name>W5MWU7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWU7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012856"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012882.1">
        <property type="protein sequence ID" value="ENSLOCP00000012856.1"/>
        <property type="gene ID" value="ENSLOCG00000010473.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074908"/>
    <dbReference type="HOGENOM" id="CLU_016218_4_3_1"/>
    <dbReference type="InParanoid" id="W5MWU7"/>
    <dbReference type="OMA" id="SHSCAVA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010473">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000004">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="355" mass="39195" checksum="4F52A37E895981F5" modified="2014-04-16" version="1">MPRADKETDLSERLESFLSDLKRGGSGSGPPRGSGETARETAALLRKITAQARWSSAGDLMEIIRKEGRRMTAAQPSETTVGNMVRRVLKIIREEYARSRGSSEETDQQESLHKLLTTGGLSEENFRQHFPPLKANVIEAINELLTELEGTTDNIAMQALEHIHSNEVIMTIGRSRTVEAFLKDAARKRSFHVIVAECAPFCQGHEMATSLAEAEIETTVITDAAIFAVMSRVNKVIIGTQTVLANGGLRAVNGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDTFHKFVSPQEVLPFTEGEILSKVNVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHRNDSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXA8</accession>
    <name>W5MXA8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spliceosome-associated protein CWC27 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the activated spliceosome B/catalytic step 1 spliceosome, one of the forms of the spliceosome which has a well-formed active site but still cannot catalyze the branching reaction and is composed at least of 52 proteins, the U2, U5 and U6 snRNAs and the pre-mRNA. Recruited during early steps of activated spliceosome B maturation, it is probably one of the first proteins released from this complex as he matures to the spliceosome C complex. Component of the minor spliceosome, which splices U12-type introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXA8"/>
    <dbReference type="FunCoup" id="W5MXA8">
        <property type="interactions" value="790"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013017"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013045.1">
        <property type="protein sequence ID" value="ENSLOCP00000013017.1"/>
        <property type="gene ID" value="ENSLOCG00000010631.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155967"/>
    <dbReference type="InParanoid" id="W5MXA8"/>
    <dbReference type="OMA" id="DDWYDVY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010631">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22288">
        <property type="entry name" value="CWC27_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01925">
        <property type="entry name" value="cyclophilin_CeCYP16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000007">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CWC27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="19"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="438"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="479" mass="55199" checksum="9986F11B01555DF6" modified="2014-04-16" version="1">MSNIYIQEPPTNGKVLLKTTAGDIDVELWSKEAPKACRNFLQLCMEGYYDGTIFHRVVPEFIVQGGDPTGTGSGGESVYGRPFKDEFHSRLRFNRRGLVAMANAGPHDNGSQFFLTLGRADELNNKHTIFGKVTGDTVYNMLRLAEVETDQNERPLNPHKIRSTEVLHSPFDDIVPREIKKVKKEKDKEEGKKSQAKATKNFSLLSFGEEAEEEEELVNKVSQSMKGRSKSSHDLLKDDPRLSSVPAVDSKRKSSKRTSEDSSEDLEEEERGSGSDEDAGERERMKDRISKKLKKDKMAEVKQEPREEEQERKTSRSDELRKEARQLKKELLAIKQRKEQNVKEEEEEEEGDAESQETPPNIAVAEYLEERKKYEDLRKQKLKRGAGREAQTLALLNQFKSKLTDAINEAPEEDTEEVPEDDDKGWLSHVLHFDEQTRKVKDANMQDEDTFEIYDPRNPVNQRRREESKRIMRQKKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXY5</accession>
    <name>W5MXY5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative homeodomain transcription factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXY5"/>
    <dbReference type="FunCoup" id="W5MXY5">
        <property type="interactions" value="950"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013244"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013272.1">
        <property type="protein sequence ID" value="ENSLOCP00000013244.1"/>
        <property type="gene ID" value="ENSLOCG00000010802.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQGQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011648"/>
    <dbReference type="HOGENOM" id="CLU_013937_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXY5"/>
    <dbReference type="OMA" id="KMWQTRE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010802">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039775">
        <property type="entry name" value="PHTF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021980">
        <property type="entry name" value="PHTF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680:SF8">
        <property type="entry name" value="PROTEIN PHTF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680">
        <property type="entry name" value="PUTATIVE HOMEODOMAIN TRANSCRIPTION FACTOR PHTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12129">
        <property type="entry name" value="PHTF1-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="634"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="PHTF1/2 N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="332"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="749" mass="84514" checksum="8F7455BB11E4012D" modified="2014-04-16" version="1">MASNEKDAIAWYQEKIGAYDQQIWEKSLEQTELKGLRNKPKKRGRIKADLIDVDLLRGSTFSKAKPESPWTSLTRKGLVRVLFFPVFFQWWIQVTSKCIFTWLLLLYCLQVLAVLLYLETSAVNISELVGPMCLMLLLGTVHCQIVSTESSRSPAAGPARRRRQLEDIQYLKPSKRYAKSSLKNTNQRTQMETVFKCENRSVGIAVCSLRRPRKERSPGDKTAQRDGSLNAQADRPPELQHCLLGQNDKYSKAPKRQDFSKSSVMGVSDDYSSEDDAELLPLRRRRVEEAPADSVLRSRRALSMHNSPLSQGSSRLRQCLVTVSGRYGNMVFPAAQEERRPASRGAGGSPRRPSDSPRLGSDTDDMLCEELLQGPDSASSGSSDSGEGGPWYNGLPPAVFRTTEEDLLQQQSHLSWLQSCHPSTDRVSTIVWENGECKKVDMSVLEISAVIMSRVKDEEQGLGYLMLGGGVTVVLALLPLIFQMTRQLDVALLASLSAAELLHLACGPPDPRGYTLLLITLLERVCLSWLFFFMMCVAERTYKQRLLFAKLFSHLTSARKAKKSEIPHFRLKNVQNVKIWLSLRSFLKRRGPQRSVDVIVSSVFLVALSIAFVCCAQFLQSHHTFLDSMYNWELLIWESSLVIFLLRLATLGSETNRKYSNMSVLLTEQINLYLKMEKKPSKKEELNIVNNVLKLATKLMKELDTPFRLYGLTVNPLIYNITRVVILSAVSGVVSDLLGINIRLWKIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ15</accession>
    <name>W5MZ15_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">U2 small nuclear ribonucleoprotein A'-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200300.1">
        <property type="nucleotide sequence ID" value="XM_015344814.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ15"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013624"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013653.1">
        <property type="protein sequence ID" value="ENSLOCP00000013624.1"/>
        <property type="gene ID" value="ENSLOCG00000011091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686263"/>
    <dbReference type="KEGG" id="loc:102686263"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153289"/>
    <dbReference type="HOGENOM" id="CLU_109943_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ15"/>
    <dbReference type="OMA" id="FYDYLQY"/>
    <dbReference type="OrthoDB" id="10251250at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011091">
        <property type="expression patterns" value="Expressed in muscle tissue and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043313">
        <property type="entry name" value="LRMDA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282">
        <property type="entry name" value="LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="21333" checksum="6179F32737EBEC7A" modified="2014-04-16" version="1">MLGCRRLSFAYQNLVEIPCSTILQYQDTLEVLDLSYNLLEESPAVLGELEKLTTLILDCNNYTSHVKFPYMPSITTVWINKNRIKNLPIFVEELRRKFPNIRFLSMMNNEAAPSYFNGGSLTQYLDYRQYVISQLPRLEVLDDKEVLKEERDAAKRTYKIQERRDTYRKKRELNKGNMTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZL7</accession>
    <name>W5MZL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Focadhesin</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031582">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031583">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MZL7">
        <property type="interactions" value="573"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013826"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013855.1">
        <property type="protein sequence ID" value="ENSLOCP00000013826.1"/>
        <property type="gene ID" value="ENSLOCG00000011240.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQKG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004438"/>
    <dbReference type="HOGENOM" id="CLU_002970_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZL7"/>
    <dbReference type="OMA" id="GQLFSWF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011240">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060147">
        <property type="term" value="P:regulation of post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022542">
        <property type="entry name" value="FOCAD/RST1_DUF3730"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045163">
        <property type="entry name" value="Focadhesin/RST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021392">
        <property type="entry name" value="Focadhesin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16212:SF4">
        <property type="entry name" value="FOCADHESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16212">
        <property type="entry name" value="FOCADHESIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12530">
        <property type="entry name" value="DUF3730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11229">
        <property type="entry name" value="Focadhesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3730" evidence="3">
        <location>
            <begin position="489"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Focadhesin C-terminal" evidence="2">
        <location>
            <begin position="1209"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1793" mass="199313" checksum="773721BFC21BC14D" modified="2014-04-16" version="1">MSEGLRKQLEFPSSLIQAQAVRSLVAAVLKEKGLNEKIAQSSNQSPALELLWEKCCSDSVLVRSACCDALVLLVEQNHADLQYVLNSILNLIPSASNVQGLIKVIGKLLQIQSLQHLQDGKAVGCPYTIRNPPHPYVTILENRPDCWPVLLREIGDYIQQCIKKMEPAYVAMLTPFLRYVYCEPLHLPEHAEFRQNLLRVLVPHCQEKGEESEPSSVAQCVIRSLFDLIPHLQVVTVTQVVELGLFVKELSSNLLKHAKFWKTELCQLSLQLLCACELNLRITGEFSALIDLIENHLEVCSEDFPSEQMITGLALLLLKAPATQQIKILNLAFKIITSSEMPVLPAAAPILVLPLLQILSCTALMEALSDSQIRETNQHLASTLLQTVQGHTFSQKEDTIKLPFPICSWYSTLLITTDALHRLNEDQSAACKWLFSVHSALPLNESIPEHVPLLLSYLIVREEGEGLEVALRTVAELAKADPSQVPNLIPVLMFKLGKLSDPVLSCAVLYALPSLGTHKFCVPQLLQALQVLGSASKLRPVVLRLMTSLWIKQDRVYPELQKLMAMLDKSSVVFGKEEQWEQILAKAACIRDICRERPYQHGGDMLAAITETLSQCTKNDQATPAAFVLQGLLELCRAEVVDIRSTWNALSPKLNCDSRPIVVKALTELFALVPVLSVKTEEYEKFKDQVVIILWSYTLNKDPLIASCGYRALSNFPEGVHTILHLPEQARPPVKTPDSEEEINEDEEKEEDLSIPGASYIKLLGLTPPAVLPALEVFLTSLVRQEMVEMPRGVYHSALRGAASRSDLGKTVAGIPGFILKTYEKNKQPGLKPGLAAGLLLCYDLPIQTDRDGRPINRFLVSRGRSFQQMLAALIHEVNIQPSEWHRSILLPQGWRGFMSRAYHAVLQLCNGGIVATSKNHFSINKHTYSFSIISFHPYFHFQLTQNYSRGLSTGIQGKPKTSLLLIYSVRHTVGKHEACTGGVFSQVLILTNLYVRLDQKQQVSIPFFIHTSRELFRVFAHGQYKRHVECIIYMYDLGASSTCPFRYDFIFFCIPSTFPLLELTLQRLHGLPTKKWARWYSNQKPIFPGMFCPNICLSQYSDISGQKMNLFLMKSLDVLEGCSFDTSLEYNTGCILGVGLVLSLLSHSSQTESRVHVAATLEKLLCSLEDSSSQGRMLQEXLAYSVACVCVSAFSTGIIDANKAEEIMNNLRLMTEKSQQTPGFALSLGITVHGLSACGHGKAEDFYFRLLGAWIKILLTEGCPTMQRLAAVNGLVALVGSESSLIQLKSESGQSPQQQSRLNEVIRAITQVITFSGAVGLQSNASCLVGHLHLANLSLGQTRTSVPPDFGYLSERSVIRAAVDYLIEAGRKGPEHLHPKLVNVVLGPLASVGSSFQYPPVNWGAILSPLMRLNFGNTFAKICQEIAIADNLRSSSFLAGLNPLLCKLLKLKTRAYLYKSVSVWMKHVPDDKLQSFVEVLGVQHLTASSRQQSPDLCVAVLEGLEQAMKLPNPTQFCWGVLRSTTERIFMLLPDEVQDDEVELYVRIATCLSEMEDTEIDRITRVTKANLVKTGFTMAYLVSQGRVPLLGLNDVIAAAIGSCHQEAVSSLLVRSFYQSRLAASPNTGVLKRMEWLLELMGHIRNVAYGSTPVENGDVKQATDFLLQLFCAAVVSWADHSLPLLLGIGAKWLPWKEDNAVPSSPQARFGNSSADEQLALQCSKGLPYSIQLLLAKEPWKGQTHKFIDWFFSILECPKQGLSERSIRTVKGALLALRYLPEFKKKVVWTRAYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0N7</accession>
    <name>W5N0N7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Proteinase-activated receptor 4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632095.1">
        <property type="nucleotide sequence ID" value="XM_006632032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0N7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014196"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014225.1">
        <property type="protein sequence ID" value="ENSLOCP00000014196.1"/>
        <property type="gene ID" value="ENSLOCG00000011550.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692611"/>
    <dbReference type="KEGG" id="loc:102692611"/>
    <dbReference type="eggNOG" id="ENOG502QU4Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244840"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5N0N7"/>
    <dbReference type="OMA" id="PCANDSD"/>
    <dbReference type="OrthoDB" id="8716763at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011550">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000040">
        <property type="entry name" value="Coagulation factor 2 (thrombin) receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003944">
        <property type="entry name" value="Prot_act_rcpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF22">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01430">
        <property type="entry name" value="PROTEASEAR4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867079" evidence="14">
        <location>
            <begin position="21"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="83"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="138"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="372" mass="41583" checksum="3872BF382686B5B2" modified="2014-04-16" version="1">MNSLLFGLSLFFGLFGLIWTQQHVNERKCGPPPSRSLTLYQKCENGVISNFLNASSQSTLTGPVATLIIPIVYTVVFLVGLPANAVALWVLLTKVKKLPSVILLVNLALADLLLILFLPFKAVYYFLGSHWTFGEPMCRIVTAAIYGNMYCSILCLTLISIDRYISLVHPFSAKSFRSRTLAISTCAVAWATAIAAMAPFVLNKQSYPINGTDLIICHDVLPESLNTGLFIYYFSFLSGFGFLVPFISILFCYVSIVFSMIRHKERFARTIKITILVLTVFTVCYIPSHVILLLHYFRDKAWQDPLLYLAYMIGLALSTCNSAIDPFLYYYVSDDFRAKVKGALVGLRTQESTQGSQTTQQSSNKLKSEEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N306</accession>
    <name>W5N306_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glucose-6-phosphatase catalytic subunit 1</fullName>
            <ecNumber evidence="5">3.1.3.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Glucose-6-phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate + H2O = D-glucose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16689"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="3.1.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glucose-6-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.9" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01023053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N306"/>
    <dbReference type="FunCoup" id="W5N306">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015015"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015044.1">
        <property type="protein sequence ID" value="ENSLOCP00000015015.1"/>
        <property type="gene ID" value="ENSLOCG00000012200.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS9B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183150"/>
    <dbReference type="HOGENOM" id="CLU_052517_0_0_1"/>
    <dbReference type="InParanoid" id="W5N306"/>
    <dbReference type="OMA" id="WCEHPEW"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012200">
        <property type="expression patterns" value="Expressed in heart and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048878">
        <property type="term" value="P:chemical homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03381">
        <property type="entry name" value="PAP2_glucose_6_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000010">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016275">
        <property type="entry name" value="Glucose-6-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591:SF3">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000905">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="19">
        <location>
            <begin position="58"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="358" mass="40155" checksum="4DA1DB63261B46BD" modified="2014-04-16" version="1">AMDAGMDLVHDYGVGAVQYLQTHYRDAQGWFLLVSFIADLRNTFFILFPIWFHLRESVGVKLIWVAVVGDWLNLVFKWILFGERPYWWVHETPYYSNSSVPHVEQFPVTCETGPGSPSGHTMGSAGVYYVMVTSLLAILVKKHKPSVKNWCVRASLWTLFWGVQVCVCLSRVFLAAHFPHQVIAGVISGMIVAEAFSRVQWIYSASLKRYLLTTLFLLVFALGFYLLLRVLGVDLLWTLEKAKRWCVRAEWVHMDTTPFASLLRNLGTLFGLGLGLHSPLYSESCSGKRGHSAPFRLGCIAVSLSLLHLFDSFKPPTHMVALFYLLSFCKSAAVPLATVGIIPYCLSGALSAHSKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5N354</accession>
    <name>W5N354_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">trypsin</fullName>
            <ecNumber evidence="12">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01017724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N354"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015063"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015092.1">
        <property type="protein sequence ID" value="ENSLOCP00000015063.1"/>
        <property type="gene ID" value="ENSLOCG00000012242.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695911"/>
    <dbReference type="KEGG" id="loc:102695911"/>
    <dbReference type="CTD" id="3426"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151042"/>
    <dbReference type="HOGENOM" id="CLU_006842_19_6_1"/>
    <dbReference type="InParanoid" id="W5N354"/>
    <dbReference type="OMA" id="YECQQPK"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012242">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000053">
        <property type="entry name" value="Neurotrypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048722">
        <property type="entry name" value="CFAI_FIMAC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048719">
        <property type="entry name" value="CFAI_KAZAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003884">
        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF83">
        <property type="entry name" value="COMPLEMENT FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21286">
        <property type="entry name" value="CFAI_FIMAC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21287">
        <property type="entry name" value="Kazal_CFAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
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        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
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    </dbReference>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869211" description="trypsin" evidence="16">
        <location>
            <begin position="22"/>
            <end position="623"/>
        </location>
    </feature>
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        <location>
            <begin position="91"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="18">
        <location>
            <begin position="140"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="17">
        <location>
            <begin position="381"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="623" mass="69138" checksum="BEF0989F9DF64961" modified="2014-04-16" version="1">MKLVFLHFLNLLFIGNGRTESQPEDNVPSHPLDRPNTQITTTAASTKIIPAKLLQDEYLDRETCGTKGFSRLSCQKVFCPPWMRCLNGECICKLPYQCPKATKPACGTDKREYRSFCQVKALGCIKKDVLFSHFGPECKSKLFDCSLGPKNVLKVERRNETFLICGSRWTIAEANVACRNVKREEKGAEAASVVKHEEIEHQSGLSKRCIHVRCTGEESSLAECTFYKTDNTDTEPTIATVNCYNRIKNCTNFEFRCVNGKCIDLNRTCDGTNDCGDRSDEVCCKKCRPGGFHCSSDVCIPNQAKKDGVMDCIGGDDEYTEESNVNFGRSPKPESIPTTEEEIIATTKEEVEAARKAVSTLECGISNYTKPNSTGVRQKRLLGGHTAEKGQIPWQVAIDDEGEINCGGIYIGGCWVLTAAHCVRPKPQLYRIKVGVWSTLKVDTYTDSLPVENVIIHPDYNPSTYQNDIALIQVKNIYNLPECIERTPSTASACVPWSEYQFKPGDNCIISGWGRSHGNKKVYTLKWANIEIIDNCSGIYGPRYFKGMECAGTLDGSVDTCQGDSGGPLVCKDKSGVSYVWGVVSWGEKCGVVGHPGVYTKVAHYFDWISFHVGRPTISKFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N378</accession>
    <name>W5N378_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Family with sequence similarity 13 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N378"/>
    <dbReference type="FunCoup" id="W5N378">
        <property type="interactions" value="444"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015087"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015116.1">
        <property type="protein sequence ID" value="ENSLOCP00000015087.1"/>
        <property type="gene ID" value="ENSLOCG00000012259.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183033"/>
    <dbReference type="InParanoid" id="W5N378"/>
    <dbReference type="OMA" id="SSITEQX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012259">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04393">
        <property type="entry name" value="RhoGAP_FAM13A1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF16">
        <property type="entry name" value="PROTEIN FAM13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
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    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
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        </location>
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            <end position="285"/>
        </location>
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        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="725"/>
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        </location>
    </feature>
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        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113141" checksum="0AD00042D7C694F2" modified="2014-04-16" version="1">TEGTRLAVEAMRKSLSPSLSDSLSTTRVFGIPLEEVQQSGQPGQEVPLLVKHIVEYIEEHGGLDLEGLFLVNGSAERVDWLRQRYDNGEEVDLEKEADLASAVSLLRLFLQELPEPVIPASLQAHILQLYQDCNTEEELARNMKYFLQQLPQVNYCLLRFLCRFLSNVASLQEESWSTGALAAVFGPDIFHIDTDVEVLKEQEPVSRILADLLENQEEFFDSEEEDFSTTNDYSSLNEQLASSIISSSVRRKRSLPIIGEDSPEDEQPKEPPGLTQVTPSSSSDTPFPHMWKVPEPSLQIPEEKLTVGKKLCVETIGIADLGFLNIYCIYVSEEIPRQLNNFFSSSHISPISILPASADIIERTIRAAVEQHLFDLQSTIDQGISSYESRGPPSQEDERNQQARAPSCALQLVRHAGSHGDATRERIGEGGSEEPSEGDAEATQPGKETAEAAGEMTESEQAAGDPSAEIATLLENCGFLINIAHCNYTKASFRYQAVEAKRDFTDGWDAECVASPSSLITARLLSLLQVPSGEEPDLNANSGSGGCPTPPFDLRGIFQDGEWEDPVPAFKSWQEDRESGEAQLSPLAGRMVPTPSEEDAHPLLARRFLDFGHSQRFLHQDPDATSPAKASSHGRPRRASFSSKESGKGDPVPHQLTKKLQNLKKKIKQFEEQYEKERNYKPSYRDKAANPKVLKWMTDLTKVRKQIRDTRQKASEGDLAPQPRPRSNTLPKSFGSTLDQGASEGSSEGEAQECRPTREETLELIQRRLKGKREEDSRPEDVRKMTKEQLSGEKTVLQKNLLFYESIHGRPVTREERLVVKPLYDRYRLVKQMLTRASITPIIASPSSKRRSQMLQPIIEGETAHFCDEIKEEDENEEEEGVFMETSEVTIVLDPAAQPCSPQAQLDGEVNPKLEESGGKLALDLRLSSSNASSMPELLEQLWKARADKKKLRKSIREFEDEFYQQNGRNVQKEDRAPMMDEYKEYKRIKAKLRLLEVLISKQDSSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3D6</accession>
    <name>W5N3D6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SRC kinase signaling inhibitor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3D6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015145"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015174.1">
        <property type="protein sequence ID" value="ENSLOCP00000015145.1"/>
        <property type="gene ID" value="ENSLOCG00000012303.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPNH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157961"/>
    <dbReference type="HOGENOM" id="CLU_002507_0_0_1"/>
    <dbReference type="InParanoid" id="W5N3D6"/>
    <dbReference type="OMA" id="DRMREQX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012303">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF5">
        <property type="entry name" value="SRC KINASE SIGNALING INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="206"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="481"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="835"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="965"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1110"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="722"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="842"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1054"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1165"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1179"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1213" mass="133600" checksum="C9BB7D27309F42E4" modified="2014-04-16" version="1">MISTDDVEYPREYRTLGNGTRRFSNVGLVHTSERRHTVIAAQSLEALTGLQKADMERKRDAFMDHLKNKYPPQHPPSPSPSHGSMRGQPDRVREQLIRAAILLSHLLKQCHLSPTNLSFKEIHKVCNSLLCLFNVSNTRSPRHSQSTQSGLADQASKLSFASAESLETMSEADIPLGFNRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGDETRRVHITHELSSLDTLHALIVHMFPQKLTMGMLKSPNTAILIKDETRNVFYELEDVRDIQDRSIIKIYRKEPIYASYPTAHLANGDLRREMVYTSRESSPTRRLNNLSSSASPPSGSPSRSRLSYSGGRPPSYAGPPHPHAHHPSSGQPPAGLSPSPSAILERRDVKPDEEVSSKNMVLMKNEGLYADPYSLVHEGRLSIASTQSLAAIGDPFTFPVSGGLYRRGSVRSLSTYSAAALQGELEEALYKPGGPLYSDSYASASTLGMGFRLPPSSPQKIPDVQLRDRDSYSGPPSRASPVRQSFRKDSASSSVFVESPKSRSGSGSDPLCGPGGPSGEGRGTPGYNSPQAANDSETRERMEAMEKQIASLTGLVQSVLTRGPDSDSTEKTETASDGSGTGIWLAALTPSAPSAGAAMPPPPAGATQATAVTRLQMQLHLHDLQQNATDLRDQLSQLRKMQLQNQDSVRSLLKRTETEISVRVTDALRKQEDPLQRQRLLVEEERLKYLNEEELIIQQLHDLEKSVEEIQKDSSVNHRLVTVQELEEKALVLRKLGETLTELKNQFPSLQSKMRVVLRVEVEAVKFLKEEPHRLDALLKRCKTITDTLTTLRRQVNEGIWKGQEDFASQSQKHTDDFPKNTDFDIPTSPPLNFSDLGGSASLSSWTPHSGPGLNPSSTHHDSPQHGPLKNRPLEELSGRRGVDKSVSVEAAERDWEEKRASLTQYSAQDINRLLEETQAELMKAIPDLDFAAKQAKPLAAPTTQSSTPISEIRPSKPQHIAHKLSSKSEPSARRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRSGEVIVTSKTIKKSESEETEAQKPHVKLRRTVSEAPRPASTPPVIASGVKDEDDEEKIIAELEVFQRAPVKAKVSPPHSLPSTLRRATPPPSLELWPPGAPGRKNQYQTTGGGSSGSSKGLHSATSRLKNIQQSSPEKSKAGKQREDFLKIQGQQQPCPVWVSTLLGPQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4F0</accession>
    <name>W5N4F0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway. Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-serine(in) = L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:35031"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4F0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015509"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015538.1">
        <property type="protein sequence ID" value="ENSLOCP00000015509.1"/>
        <property type="gene ID" value="ENSLOCG00000012590.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234641"/>
    <dbReference type="HOGENOM" id="CLU_039425_1_0_1"/>
    <dbReference type="InParanoid" id="W5N4F0"/>
    <dbReference type="OMA" id="TIDDVWQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012590">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF20">
        <property type="entry name" value="SIDEROFLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="325" mass="35878" checksum="C159B97E7D638C01" modified="2014-04-16" version="1">RGRMPGDLPLDIDIKEPRWDQGTFVGRAMHFFNVTNPLNVLLSSDTLEDARRTVENYKLGIVKPGLTEDELWRAKYIYDSAFHPDTGEKMILIGRMSAQVPMNMTITGCMLTFYRTTPAVVFWQWVNQSFNAVVNYTNRSGDAPITVNQLGAAYISATTGAVLTALGLKSLTKHFPPIISRFVPFAAVAAANCINIPFMRQRELKFGIPVTDEEGRRLGESTIAARQAIAQVVVSRIGMAVPAMAIPPVIMNALEKKAFMKRFPVLNAPVQVGLVGLCLVFATPLCCALFPQKSSMKVSSLEPELQAKIQEVSPHTSRVYFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4J4</accession>
    <name>W5N4J4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">LIF receptor subunit alpha a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4J4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015553"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015582.1">
        <property type="protein sequence ID" value="ENSLOCP00000015553.1"/>
        <property type="gene ID" value="ENSLOCG00000012627.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQF6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165259"/>
    <dbReference type="InParanoid" id="W5N4J4"/>
    <dbReference type="OMA" id="FFLYGCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012627">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="Leukemia inhibitory factor receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leukemia inhibitory factor receptor alpha a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Oncostatin-M-specific receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR048497">
        <property type="entry name" value="LIF-R-like_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040817">
        <property type="entry name" value="LIFR_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
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    <dbReference type="Pfam" id="PF00041">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21177">
        <property type="entry name" value="LIF-R_Ig-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF17971">
        <property type="entry name" value="LIFR_D2"/>
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    <dbReference type="Pfam" id="PF25552">
        <property type="entry name" value="LIFR_D4"/>
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        <property type="match status" value="5"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867247" evidence="13">
        <location>
            <begin position="27"/>
            <end position="950"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
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        <location>
            <begin position="324"/>
            <end position="420"/>
        </location>
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        <location>
            <begin position="422"/>
            <end position="519"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="621"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="848"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="907"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="950" mass="105450" checksum="86F0D23B3BB0D2F7" modified="2014-04-16" version="1">MQTPLCFLNGLWWFLLSAVLVRESVCEEDLSLPVPQGVTVKPDYQSDILSVEWEDDSSFRSSSEQYEIEVLLTEKMEVVHRDVYKVLLNNTNKNQWMWISPMPLNCTSHSLRIRSIHNHKKSKWSSLVTIQGSDTKDVDETAVFPIDRVVAVGTNLTFCCVAGKKDNVTDLSFQGKLGTTYLSNRTLAAQKKLPLPSISSGDNVICISPSSIGGTVVFVGYAPRDSDLVCETRDLASVECYWNTGNSTYLYGPRRTIYSLTERLSGEGNCSEANVIHGQKYCCKHVVSPGQQQYVFTLRAVNPLGAVELSDSLDLHHRGHIVAEAQKASASCKTWTSARLVWDWPKNYDRLSFVCQVEMYSQDHAEVRNYTLDKKSSWNLEMSHLLPGEKYSVRVRCGVAEDFWKWGDWSHIKQFQTLEESPVKALDIWRNIIKEEDGQKVVIAWKPLSKRESNGKIRQYEVTWKSLTDGSEQSFTLPPSDTSAELNLSNRDYVISVRASNSAGSSPPSILIVPELLLVNNSVPSVVCAANGGFHLSWSPNASVDCGYIVDWCPTHRLQNCSLYWVKVPLGSTNAVIKSEDFKPGVQYSFSIYGCTKNRPYLLERKIGYGQELVFKVPSEPVKNLKAEPRSANEVLLQWEDIPLEKKHGFIKGFIIYASNKTGRSIIAANITNPDARSYVLKRLHSSVYNFTVKAYTAAGEDDGGATVSVFMPGPTDGLIAIILISLGVMAVSSIILTVVCYKKRNWVMQTFYPDIPKPEIRGEQWIPGEGSFVNPPMNVDACDTSHVCVVGDVLVEQKESDTEEPVVDDNDTGCSSQTILNYYGQVYNGGAGTSSLESNSTEVTYTNIQPSSSASASSGYQPQMTRGPDPEELGPPLAMPLICDPAGYQPQANPKDWTVESPGPASLNSSLGSPTSINSTQFLLPDQGPEDSQEPQFSTWFHNFLSMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4K3</accession>
    <name>W5N4K3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4K3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015591.1">
        <property type="protein sequence ID" value="ENSLOCP00000015562.1"/>
        <property type="gene ID" value="ENSLOCG00000012643.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SDDU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016954"/>
    <dbReference type="HOGENOM" id="CLU_881496_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4K3"/>
    <dbReference type="OMA" id="WRAANNS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012643">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037496">
        <property type="entry name" value="BEND6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BEN" evidence="7">
        <location>
            <begin position="204"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="34681" checksum="5E2FAC827E665BCA" modified="2014-04-16" version="1">MQDEGTRVPSGSPAEPGPAVGQRRTFATAYSDSDSESEQEPTRKKLAPDLAAPAPSSINILAQLELSLQREQAEHTDPQVAGAERGDSDWGEDEALQQRSKEDLIAEIKALRAQLAQSACETRRLRQCLTVFRVLPKAVRNFTRLVDRAESLLQAPGPGGLDQPSGDPSTLGDAPMYPSPSSLSSASASSSSSPLSTPGSPSAHNRKWIKIEKWQIERFNKSTPQKFVNDLMQSLYTTEFMATHSVTGARSSSSKYRETKPAMDRAEVQAILDVTKHFFPDMTDTLIRRMMGQKLNNCTKKFAASKVKTELYPLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N556</accession>
    <name>W5N556_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hyaluronoglucosaminidase</fullName>
            <ecNumber evidence="4">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CEMIP family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01026061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N556"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015765"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015795.1">
        <property type="protein sequence ID" value="ENSLOCP00000015765.1"/>
        <property type="gene ID" value="ENSLOCG00000012806.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUM7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153636"/>
    <dbReference type="HOGENOM" id="CLU_005606_0_0_1"/>
    <dbReference type="InParanoid" id="W5N556"/>
    <dbReference type="OMA" id="YPRNTIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012806">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13938">
        <property type="entry name" value="PANDER_like_TMEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052252">
        <property type="entry name" value="CEMIP/CEMIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055400">
        <property type="entry name" value="CEMIP_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039473">
        <property type="entry name" value="TMEM2_PANDER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535:SF24">
        <property type="entry name" value="HYALURONOGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24605">
        <property type="entry name" value="CEMIP_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0430">Lectin</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="G8" evidence="13">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1277" mass="141528" checksum="037CA9E5E913A815" modified="2014-04-16" version="1">VSDRCPDKNPYLLSWRPGHDGSRKVVIGAGLFLKLESSATVHSIVIEGGGVLVFADNPEDSIILRTRYILIRDGGVLHIGSEKCPYNGSATISLYGMSTEGLTVENFGGKFIGVDKGGTLEMHGKKPLSWTLLARTLHPMGLQHGPYQFERYWGSRGINVRVVDDGTGQVLSSERFDTHMMANESRRLKEFLSNQPAGVILAMAVGDSAAKSLSLEAREYILEVLGSKFIKHLGYRQPWALVGTLRGGPFSTTENRRPYTMSGTTGAAIARREFQTYDGTRFTVTAYSEWSGSPHIGFKVEALKGIVVDLVDDVTSWVPGDRIVIASTDYSMHQAEEFSLLPCPECKTNQVKIDGKPRYLHMGEIVDGIDMRAEVGLLSRNIIVKSEMESSCYGENHCDIFNYDTFGGHIKIQKGFRSFHLSGVELTAMGQQNLGSYPVHFHLAGDVDQRGGYHPPTSLENLSIHHGFSRCVTVHATHGLLVKDVVAYDTLGHCFFLEDGVEERNTFYHNLGLLTKPGTILPTDRDEPMCLRIRKNVFGNYRPVPSTDCMAVSTFWIANPNNNLINNAAAGAQDVGIWYIFHRVPTGLSEGLYPEGKTELTPLGIFYNNRVHSNFKAGLFIGKGVKTSNASAANPREYLTIDNARYRPHQDADPAKPRVPAVIDGLVAFKNNDHGAWARGGDITFQNCGFADNGIGLTLASDGTFPTDEGSSLEVTKSIFVGESSNVGSHGGQNSYWGEGANREYRTLPRNKTFPIRGFQIYDGPVRLTECSFKKFTPTADRYSSALGFFMKNAWQVSPQNNVSSVKMERSVGLNVFFGKPGQWFGANDLDGDKTSIFHDLDGSVTGYPDSYIARVDNYLIHHPGCLTVPRWNGVICSGQYAQLYIQARRPENLTLLIARETYSSRPLALRGVNRGAPYQQYQPVVMLQQAYVMQWKGVSPEEVILYPINFDRGNWIQVALCYPQGTTFRIVSDINQRQSGKVHSVEHYTPASSIEAVLENPSKRLFYFDSQSGYLFLHLQASHPRKGHSYCSKLGCERVKILARSVSTTPQACPPNPFPVKSRGIPAKRLWVSQRVGTPCSLCGAQQVAISSEPFREYVRVQVLSLSRANIVNGLKSAFIKVNDEVFLFRKQGLLFVVVDACTGEIKSRRHFEILTVSQASSAINSYIISAVKDRSIVLVCSRDIKDLPRDSGIPAFIKLGSSKPIGLFRKGSFALLGYRGQSKPSWIKIVNRSNGKEAASIQHYVPLRLKEYKCPGQGREYRKDEKLLKGLLQQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6F7</accession>
    <name>W5N6F7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:ch73-335l21.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627390.1">
        <property type="nucleotide sequence ID" value="XM_006627327.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6F7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016216"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016246.1">
        <property type="protein sequence ID" value="ENSLOCP00000016216.1"/>
        <property type="gene ID" value="ENSLOCG00000013165.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690862"/>
    <dbReference type="KEGG" id="loc:102690862"/>
    <dbReference type="eggNOG" id="ENOG502S42D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000112287"/>
    <dbReference type="HOGENOM" id="CLU_1547083_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6F7"/>
    <dbReference type="OMA" id="CPRELHC"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013165">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF14">
        <property type="entry name" value="RING FINGER PROTEIN 227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="185" mass="20621" checksum="3181AEF909F46397" modified="2014-04-16" version="1">MCTELECGICYRAYSTGRRCPRELSCRHTFCERCLFTLALSPKHLSVEDETRREDPRCDTAIIVCPLCRRSTSVSSPSGVRRELRVDEAVLERMEVAGVLHGYDGQSDGEDAEEEGASDAPPERDPCGTAGEESDAPLSRKGKLWRSIKRFWKKCAGTSSGTQRGIHCTSEDLKDIALMTCYTMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7E1</accession>
    <name>W5N7E1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RLR subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01002055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7E1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016550"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016580.1">
        <property type="protein sequence ID" value="ENSLOCP00000016550.1"/>
        <property type="gene ID" value="ENSLOCG00000013422.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0354">
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        <property type="term" value="P:cytoplasmic pattern recognition receptor signaling pathway"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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    <dbReference type="PANTHER" id="PTHR14074:SF16">
        <property type="entry name" value="ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I"/>
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    <dbReference type="PANTHER" id="PTHR14074">
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        <property type="match status" value="1"/>
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    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="253"/>
            <end position="433"/>
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            <begin position="612"/>
            <end position="781"/>
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            <begin position="801"/>
            <end position="929"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="940" mass="108468" checksum="D80812A7896D0E3D" modified="2014-04-16" version="1">MYELEKENLKRFNSYIVKILRPSYIKEFMVDYLRPEKAEQIISEEGISVTSAAEMLMRSLWTLDQDGWFVAFLNTLRAEGYTGLYEAIKEWDFKNIDSMEPFKHLLDRIEQSFTKQIKPTEMINHMKDCLLARECEEIMAISLQKGNIAGAQKFTECLKRSDKSNWYKTLTLALQNCNYMVAYELLDDTENEKAADDSEANEGEATVRFEYREEADVDCLSGNFNGQPGTSNSSSLAESRARRKLRKYQEELAVPAFNGENTIICAPTGCGKTVVALAICENHLKAKHERKPKIVFMATKVDVYEQQYALFRSHFMDGNEEVQVAGLCGDTGDHIPVGMIIQNNDIIVLTPQILVNAIKTREVLSLSLFTLLIFDECHNTTGKHAYNILMTNYLDCKIDKKNGPLPQIVGLTASVGIGSFKNEDEAAHNICQLCANLDVRVISTVKENQEELTSFVNIPEKVFFQVEKRTSDPFISIICRIMSNIEGLARNLYNIDSLSNIQTRDYGTQKYEQWIVDVQKRCKVLQLSDKKEESRVCRALFNYTEHLRKYNDALIINEDARTKDALVYLEAFFDQVRNAGFDETEQKITAFFDAEHKHLVQLSNCEREENPKLKELKYILDEEYRNNDQTRTVLFVRTRALAEALKNWIDENDTLKFLKPGVLIGRGKRSQQTGLYWMTLTSKKGLLDSFKSSDQSKVLIATSVADEGIDIPQCNLVLMYEYVGNVVKMVQVRGRGRAQGSKCILVSSEKARIEKEQLNIFREKIMDKAVNNLQLSREAMLRKIDILQREDKKIRDYINSAPQMPRTSESFELLCSKCKSMMCFSDDLRVLRESHHVVLNKRFFERCITKPHDKPRTYDGFSKKMKMFCKNCKFDWGIVGSYMHINDLPVIKIESFVAKNCVTNRQFCFRKWRDVTFRMKEFDLSEIRPDHLSLMSNLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N877</accession>
    <name>W5N877_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uracil phosphoribosyltransferase homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632671.1">
        <property type="nucleotide sequence ID" value="XM_006632608.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N877"/>
    <dbReference type="FunCoup" id="W5N877">
        <property type="interactions" value="612"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016836"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016866.1">
        <property type="protein sequence ID" value="ENSLOCP00000016836.1"/>
        <property type="gene ID" value="ENSLOCG00000013655.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689394"/>
    <dbReference type="KEGG" id="loc:102689394"/>
    <dbReference type="CTD" id="139596"/>
    <dbReference type="eggNOG" id="KOG1017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="HOGENOM" id="CLU_067096_1_0_1"/>
    <dbReference type="InParanoid" id="W5N877"/>
    <dbReference type="OMA" id="NLFCTPM"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013655">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061769">
        <property type="term" value="F:nicotinate riboside kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050262">
        <property type="term" value="F:ribosylnicotinamide kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000026">
        <property type="entry name" value="Uracil phosphoribosyltransferase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="90"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="282" mass="31232" checksum="8567164E4777BE2F" modified="2014-04-16" version="1">METCTDISIATEKIMPCHNQQLSSTANQENAVISSTKQVRFASSNGSSGSSDSPVCNTEAEEVPDPCQTDISGEALRQIGPQLKLLPLNDQIRELQTIIRDKTTSRGDFVFCADRLIRLVVEEGLNQLPYTECTVTTPTGHKYDGVKFEKGNCGVSIMRSGEAMEQGLRDCCRSIRIGKILIQSDEETQKAKVYYAKFPPDIYRRKVLLMYPILSTGNTVIEAVKVLIEHGLQPKHIILLSLFSTPHGAKSIIQEFPDITILTTEVHPVAPTHFGQKYFGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8L3</accession>
    <name>W5N8L3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Acetylcholine receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8L3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016972"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017002.1">
        <property type="protein sequence ID" value="ENSLOCP00000016972.1"/>
        <property type="gene ID" value="ENSLOCG00000013759.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158661"/>
    <dbReference type="HOGENOM" id="CLU_018074_1_4_1"/>
    <dbReference type="InParanoid" id="W5N8L3"/>
    <dbReference type="OMA" id="PCILITV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013759">
        <property type="expression patterns" value="Expressed in muscle tissue and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015464">
        <property type="term" value="F:acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0095500">
        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051899">
        <property type="term" value="P:membrane depolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000026">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000031">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000012">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022248157" description="Acetylcholine receptor subunit beta" evidence="21">
        <location>
            <begin position="19"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="26"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="242"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="495" mass="56537" checksum="A01966D5BBE6C2A6" modified="2014-04-16" version="1">MKGLGIVLLMCSICAVAALSDASEAEKNLIAKLFTNYTLKVLPVKNVGERLVVRVGMKLASLIDLNEKEEEMSTNVYMDLTWSDYRLSWNPAEHDNIQVLRLPAGKVWLPDIVLINNNDGVFDVSLRVHVQAYSDGRVTWSPPALYKSSCGIKVTFFPFDWQNCTMVFRSYTYDASEIDLQYALDKEGKEIHEIIMDESFSENGEWQIRYKPSRKNVGPDLYEDITFYLIIERKPLIYVLNIVIPCILITIIAIFNFYLPPDAGEKMGLSINVLLTLTVFLLLLADKVPETSLGVPIIVNYLMFTMILVTFSVILSVVVLNLHHRSPNTHVMPLWVRKIFIHLLPQYLGLLRPKTETPLSLELSPKREVPVSVSRAADEYFIRKPTSNFLFPKPNRFQPESLTVDLRKFINGPSHYLTLPPDLKSAIDAVTYIAETLQAEKDYEALKEDWQYVAMVVDRLFLWIFVIFTTVGTLAIFMDASFNDTPQDPFMNPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8T0</accession>
    <name>W5N8T0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Y-box binding protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8T0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017039"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017069.1">
        <property type="protein sequence ID" value="ENSLOCP00000017039.1"/>
        <property type="gene ID" value="ENSLOCG00000013819.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159816"/>
    <dbReference type="HOGENOM" id="CLU_063071_0_1_1"/>
    <dbReference type="InParanoid" id="W5N8T0"/>
    <dbReference type="OMA" id="GQTQSDQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013819">
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            <begin position="1"/>
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        <location>
            <begin position="127"/>
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        </location>
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        <location>
            <begin position="165"/>
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        </location>
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        <location>
            <begin position="206"/>
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        <location>
            <begin position="230"/>
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        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
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        <location>
            <begin position="306"/>
            <end position="315"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51857"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="340" mass="36679" checksum="A30692A6A94AA811" modified="2014-04-16" version="1">MSETETPGTPAAPATADDRPPQPEKKVIATQVQGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVQGEKGAEAANVTGPGGIAVKGSRYAPNRRQFRRRFFPRAPRAAAANQVEGAGPAPAAGPASEGEGTGDDAAQQPPGPPRRRRPFFRQRFPRRRPDAQQGVAAAEEGEVPEGDTAAQQRIPRRRFRPPFRRPFRPRQPAGGQTPGTEGQPEGQAPGQQPEGQTGEQTDAAQPAQAQRPRRQFSRRRQRAADQTAPKQEVAEKPAAEPGSPVQTAKEVDGQSSPKDTPSSPKSPPPLPPESQDDEPAVEVRGSGG</sequence>
</entry>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="17537" checksum="D8692AD40241D741" modified="2014-04-16" version="1">CPAVSRMKYYYQCPSSQYSRAVPVKPVRTVRFPNDIVFQDHIRQGDLEQIGRFIRARKVQLDTIYLSGMAALHEAVLAGNLECVKLLVKYGADIHQRDEEGWTPLHIACSDGHPHIARYLLSLGASVEAVNENGEKPADLIDPDCKELVTVFKGMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA57</accession>
    <name>W5NA57_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 91</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
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    </dbReference>
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        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0338 PROTEIN"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="187"/>
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        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="241" mass="26698" checksum="746F91871FEE430C" modified="2014-04-16" version="1">MEDLDELEHPLLAGSPKHRGTCSPSDCSLLKEIQLRLEEEKKPLSPPLAWRSYCTPGELGRQQLLDPCSLPHTLNPFYPHAPLWGNTADTFSFHQKDYLETTFVDIVPGEPLDRKLLEEQKDVHSLSYGIEDESDLLPDYEDSSSDDYSDTDSDSDFSLIIPQDYLGLAVFSMLCCFWPLGIAAFYLSQKTNKASAQGDFQGASAASRQALWLAVLSIIFGVVTYVCAIAALVSYLSGKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB33</accession>
    <name>W5NB33_LEPOC</name>
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        <submittedName>
            <fullName evidence="11">Inter-alpha-trypsin inhibitor heavy chain 3b, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014801">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014802">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB33"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017842"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017874.1">
        <property type="protein sequence ID" value="ENSLOCP00000017842.1"/>
        <property type="gene ID" value="ENSLOCG00000014498.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPS2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154554"/>
    <dbReference type="HOGENOM" id="CLU_008101_2_0_1"/>
    <dbReference type="InParanoid" id="W5NB33"/>
    <dbReference type="OMA" id="DTSTSMM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014498">
        <property type="expression patterns" value="Expressed in liver and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000013">
        <property type="entry name" value="inter-alpha-trypsin inhibitor heavy chain H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF119">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VIT" evidence="10">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="257"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="580"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="68064" checksum="749D9CCACFA74DC1" modified="2014-04-16" version="1">ACLLTLRPPQGSRSDIDIYSFLIESRVASRYARTVITSRVANRADRSQEIAFQVELPKTAFISNFSMTVQGRTYVGVVKEKEAARQQYSEAVSRGESAGLVGTVGRTLEEFKTSVNVAPLSKVTFELTYEELLKRRLGKYELMIKARPKQVVSNFKIDVRIYEPQGIRSVEAQGSLTSGAQPDLLQISESEGTVAHVEFAPTVEQQQCADCAGSGLDGDLVIKYDVNRELPQGVLQVQNGYFVHYFSPANIERIPKNVVFVIDRSGSMSGRKIVQTREALLKILADVHEDDHFGLITFDSEVTPWKDSLVRASGGMLEEARGFVRQITDRGATDINAALEKGVAMLNKAKEEKTVPEGSTSIIILLTDGDPTTGVTNLGAIQANVKEAIQKRYTLYCLGFGFDVNLKFLEKMALENGGVARRIYEDSDSDLQLQGFYEEVATPLLVNVEMLYAHGDAANVTQSTFDKFYNGSEIVVAGRISDNSLEVLTAEVKAQSKDSIVTYQAQIPVAGEGVGKDFPKAEYIYGEYIERLWAYLTVQQLLEQEVLASDSDKPEIRQCAQNLSLQYNFVTPLTSMVVTRPEEGQSSVPQVAEKPSEEQAQNRRHGTPPQRPGPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB76</accession>
    <name>W5NB76_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S11, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB76"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017885"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017917.1">
        <property type="protein sequence ID" value="ENSLOCP00000017885.1"/>
        <property type="gene ID" value="ENSLOCG00000014531.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016068"/>
    <dbReference type="HOGENOM" id="CLU_072439_1_0_1"/>
    <dbReference type="InParanoid" id="W5NB76"/>
    <dbReference type="OMA" id="THNNTHI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014531">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000006">
        <property type="entry name" value="28S ribosomal protein S11, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003698">
        <property type="entry name" value="PRK05309.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="203" mass="21062" checksum="EA8A433EC36C90E5" modified="2014-04-16" version="1">MYNFSSKLCTVAGAACQRIAASLNPGSRAVSAGVSRALASSAARLQEAAETSASPQGSGEFSVCPPLPGQDSPLRWGGKKFEELPIAHIKATYNNTHIQVTDHQGQYTVRTSCGTEGFRNVKKGTPIAAQTAGISAAAKARAKGVAFVRVVVKGLGPGRMSAIKGLTMGGLEVVSITDNTPVPHNGCRPRKTQLEEVLGSINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBD6</accession>
    <name>W5NBD6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Thrombospondin type 1 domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004981">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBD6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017945"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017977.1">
        <property type="protein sequence ID" value="ENSLOCP00000017945.1"/>
        <property type="gene ID" value="ENSLOCG00000014576.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156594"/>
    <dbReference type="InParanoid" id="W5NBD6"/>
    <dbReference type="OMA" id="SQRRCMH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014576">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000039">
        <property type="entry name" value="thrombospondin type-1 domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF319">
        <property type="entry name" value="THROMBOSPONDIN TYPE 1 DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867338" evidence="7">
        <location>
            <begin position="27"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="8">
        <location>
            <begin position="937"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="984" mass="108572" checksum="5A3BA611E6F36141" modified="2014-04-16" version="1">DSQPDFFLLCRIPVLLLLIGPTLVDCQHTVEHRKVPQRIGPQQDAADGRGEVSGSWGPWGPWAACSRSCGGGVREQTRPCLAYPQEVARVPHGVPPHTLAPGRVVSALRPSVPLHRPGRPPRSNDTHPQSAGGPAQSSRPGHRTVPCFSHRGSSEKQITPGRYGYGKLPYIVPLKTDTGQGQRAPRPRRQRQASHPRRRGQGQGYRQQNPHPGYDTGNGIYRGSQSRTHHARTSIYQPAQSQYPGSGSSLLLSPHLRYPGPGVTTIKCVGAHKQYKICNTNACPSSSRNIREVQCSSYNNKPFMGRLYEWEPFNEAQGDQRCELNCRAIGYRFYVRQAERVIDGTPCDHNDSSVCVAGQCKSLGCDELLGSDKVMDKCGVCGGDNTACEVVSGVFKHSLMNIGYHKIVEIPVGATKINITEMIKSRNYLALRSRSGRSIVNGNWAIDRPGKYEGAGTMFTYRRPNEITSTAGESVVAEGPTNEVLEVYMIYQQPNPGVQYEFIVPRENLISPHPSPDRKPDKWNVAVNNILNTFVSVSLDSPGSHLNGQFGLEGDADSREEGSDHPHGTRSPSLAPEPPRRQREHNWKQLGSSECSASCGRGVRYARFGCVHRATHAEVADSLCDPATKPGAVEEACNTLACPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRTFTTQANRCQHLERPETTSSCQLKICSEWEIRTDWSPCSVPCGVGQKNREVRCVSNVGDIVADEECNMKLRPEDVENCDMGPCAKSWFFTEWGSRCSADCGAGVRSRSVVCMTNHISSLPLEGCGNERPPEAMACNNGPCETREQWFAGGWGQCSAECGNGTQHRAVVCLRSSADGFTVLQPSDCSYLERPASQQSCYLKACGAKWYHTDWSTCSKSCEGGFRVREVRCLSDDMTPSDSCEPHLRPEERETCNPDPCVSQIDENCRDKYFNCNVVVQARLCVYNYYKTACCASCTRVANRQAGFLGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC73</accession>
    <name>W5NC73_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Forkhead box A3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC73"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018232"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018264.1">
        <property type="protein sequence ID" value="ENSLOCP00000018232.1"/>
        <property type="gene ID" value="ENSLOCG00000014809.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166203"/>
    <dbReference type="HOGENOM" id="CLU_027910_4_1_1"/>
    <dbReference type="InParanoid" id="W5NC73"/>
    <dbReference type="OMA" id="HDLPEWN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014809">
        <property type="expression patterns" value="Expressed in liver and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000042">
        <property type="entry name" value="hepatocyte nuclear factor 3-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013638">
        <property type="entry name" value="Fork-head_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018533">
        <property type="entry name" value="Forkhead_box_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF201">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 3-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08430">
        <property type="entry name" value="Forkhead_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09354">
        <property type="entry name" value="HNF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="152"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="152"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="251"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="439" mass="47454" checksum="A4BB7D73969545C4" modified="2014-04-16" version="1">RLVSGMLSSVKMESHDIPEWNSFYSEANEMYSSASSMSSGLGPMNSLNSYINLNPVGSPAAMNMGYPSGGLNGGPLTAGMPGASNPMSLSPVAPSLNHGSLTQLGSPPGPLNSLSPYQSMSQPMGQLSYPSPTLGRGKEVAKPYRRSLTHAKPPYSYISLITMAIQQSGSKMLTLNEIYQWIMDLFPYYRENQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWTLHPNSGNMFENGCYLRRQKRFKIEDKAGKKGGPKSQDAGKGAQSDGVQEQPSPDPGSEGADSAHSDSSHAGSSPEEQQQQQQRSLIQLECPPPPLQGSPAMSSSPSSSHPLSQPLGGGHLLSSSLQHLDLQNDPLKSMDPHYNFNHPFSITNLMSSEQKMDLKSYQDQVMAYNSYAASSPGPSKLGYDSAAAAMDSGSYYQTLYSRSVLNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC90</accession>
    <name>W5NC90_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ovo like zinc finger 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC90"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018281.1">
        <property type="protein sequence ID" value="ENSLOCP00000018249.1"/>
        <property type="gene ID" value="ENSLOCG00000014822.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685714"/>
    <dbReference type="KEGG" id="loc:102685714"/>
    <dbReference type="eggNOG" id="KOG3576">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159359"/>
    <dbReference type="HOGENOM" id="CLU_087964_0_0_1"/>
    <dbReference type="InParanoid" id="W5NC90"/>
    <dbReference type="OMA" id="LASSXER"/>
    <dbReference type="OrthoDB" id="6508643at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014822">
        <property type="expression patterns" value="Expressed in mesonephros and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009913">
        <property type="term" value="P:epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045616">
        <property type="term" value="P:regulation of keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010837">
        <property type="term" value="P:regulation of keratinocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001250">
        <property type="entry name" value="putative transcription factor ovo-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000452">
        <property type="entry name" value="Transcription factor Ovo-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027756">
        <property type="entry name" value="Ovo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10032:SF273">
        <property type="entry name" value="OVO LIKE ZINC FINGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10032">
        <property type="entry name" value="ZINC FINGER PROTEIN WITH KRAB AND SCAN DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="29739" checksum="75E6F9AD8DA84DBB" modified="2014-04-16" version="1">MPRSFLVKKKQGPTGGWRWSDATDLPTGETAPETLSGSAATPAPPEEPCPVPAVPEPAWQGAPGQAGLSWHRETPSLPSPLQLPQATMSPYSTVPPVPRAKGRGAHAQGDFLCLVCNKVFPLQRMLTRHLKCHSMVKKHVCRYCAKGFNDTFDLKRHMRTHTGIRPYRCELCEKAFTQRCSLESHLKKIHGVRQQYAYRERRSKIFVCEDCGFTSTHGEEYYLHIRERHPASPALRRYYRKHGHAGAPGHKLGAVVLYPGSGYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDE3</accession>
    <name>W5NDE3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zic family member 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDE3"/>
    <dbReference type="FunCoup" id="W5NDE3">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018652"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018684.1">
        <property type="protein sequence ID" value="ENSLOCP00000018652.1"/>
        <property type="gene ID" value="ENSLOCG00000015156.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165969"/>
    <dbReference type="HOGENOM" id="CLU_002678_37_3_1"/>
    <dbReference type="InParanoid" id="W5NDE3"/>
    <dbReference type="OMA" id="PKQCNNI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015156">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021510">
        <property type="term" value="P:spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="287"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="528" mass="57604" checksum="BAC697B09701FA1D" modified="2014-04-16" version="1">MTGLPRFSGCPFSYVYPGETNTEPSVVLPPLAGEHMGHPTGSSLKLCPSHSLRDYPESRSGAYVGYSAPQPSDSGYASHRVAQNPRGIVGGADLSGTSMPPVTDQLATRSSHHGALGRYRDLPSYREGRSHAIFTAYHEQAHGSPDGPVVLGLSGDLLSRPPAYGQPLGGARGHRELVTQLYGLYKPMGMAVQRGAEPFLRCSRQNVKRELVCKWRGDQEGAGERPCSRTFGTLYELVTHLTVEHVGGPEQAEHVCYWERCSREKRPFKAKYKLVNHVRVHTGEKPFSCPFHGCEKVFARSENLKIHKRTHTGLGEKPFKCEFEGCNRRFANSSDRKKHSHVHSSDKPYTCKVRGCEKCYTHPSSLRKHMKLHCKAYGAKASEEREQPAAGGSPKPRDLQSDPPPKPVVLCASSCPALPGAAAAADSFATQARSGSSLATPFPPQYHKGLDCSSARSPSVLDPLLLQRGPFRTDLQFPASQPGNALAQSPRVFQSHSAPPFQKGIVNGWYTCHSAAEPCIAKHLNSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDQ4</accession>
    <name>W5NDQ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="11">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01025424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633210.1">
        <property type="nucleotide sequence ID" value="XM_006633147.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDQ4"/>
    <dbReference type="FunCoup" id="W5NDQ4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000018795.1">
        <property type="protein sequence ID" value="ENSLOCP00000018763.1"/>
        <property type="gene ID" value="ENSLOCG00000015248.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694358"/>
    <dbReference type="KEGG" id="loc:102694358"/>
    <dbReference type="CTD" id="562040"/>
    <dbReference type="eggNOG" id="KOG2849">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063825"/>
    <dbReference type="HOGENOM" id="CLU_048034_1_0_1"/>
    <dbReference type="InParanoid" id="W5NDQ4"/>
    <dbReference type="OMA" id="ERNDGGW"/>
    <dbReference type="OrthoDB" id="430326at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015248">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF33">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="7"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="290" mass="33250" checksum="328BE017EE40C5BB" modified="2014-04-16" version="1">MAKGQGINQELSSLLNELWALDENRLKPGKDYTISLQGKAGYVAPGSNSARDRASAPLFSHVNEERLRTIKTYAYFMKLLDNYEMSTGVSETVTTEEVEENNLFLDAVLNTKVMKRAHQYLVTKKQASPNVEQFKKQLYDIWFRLYHRDRSGGEDSCGFEHVFVGETKYGKEIMGLHNWVQFYLQEKLNNVDYKGYKARDNKDTPDEDDHVLNLQFSWKGLVKPVGSSFIGVSPEFELALFTLVFLMSQEKVTSTIVKVDEYLLEIVVYRHGRSIGTSYPKMLSSNNRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG15</accession>
    <name>W5NG15_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tubulin-folding cofactor E</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin-folding protein; involved in the second step of the tubulin folding pathway and in the regulation of tubulin heterodimer dissociation. Required for correct organization of microtubule cytoskeleton and mitotic splindle, and maintenance of the neuronal microtubule network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers. Interacts with TBCD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCE family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG15"/>
    <dbReference type="FunCoup" id="W5NG15">
        <property type="interactions" value="1787"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019574"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019606.1">
        <property type="protein sequence ID" value="ENSLOCP00000019574.1"/>
        <property type="gene ID" value="ENSLOCG00000015896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698504"/>
    <dbReference type="KEGG" id="loc:102698504"/>
    <dbReference type="CTD" id="6905"/>
    <dbReference type="eggNOG" id="KOG3207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063405"/>
    <dbReference type="HOGENOM" id="CLU_017716_5_0_1"/>
    <dbReference type="InParanoid" id="W5NG15"/>
    <dbReference type="OMA" id="SEESHMF"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015896">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17044">
        <property type="entry name" value="Ubl_TBCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000008">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000173">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000268">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001017">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044079">
        <property type="entry name" value="Ubl_TBCE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="15">
        <location>
            <begin position="37"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="536" mass="59542" checksum="C518E03C3AFBA037" modified="2014-04-16" version="1">MTVTDAQLSTMSEAGPGDAVGRRVSCDGERGTVRYVGKVPPTAGIWLGVEWDNPERGKHDGTHEGVQYFKCSHPTGGSFVRPRKASFGVDFLTALRQRYDMEMEQDSAEELKIANKPVELVGFETVCQKLSQLNSLEAVSLQGSEVAGAGPDNEIQQNTPNILSLDLSESLLASWDDVASITRQLIKLRELQLSYNRLHMPANPACLSHCFASLKVLVLNSTGITWSEVLVCAPMWPVLEKLCLSSNAITVLQKPVDVLQSLSLLDLSKNPLAGGDELQNIAHLPKLEKLILSNTGLSSIRFNDVGPGCKTSMFPSLKCLVVENNNISEWSFVNELEKLPNLQQLSCFNNPLMTAGKSPETVRQLIIAKVGGLQLLNKSKIQPVERRGAELDYRKMFGRVWLECGGDRDPERDRPSQAFAAEHPRFRTLIQKYGAPEEGELKQQQPFALKNQLLTITFVCPDVTEKKPIEKKLPDSMNIQKVKGLLFRLLKVPGAELRLSYTSSKMEGKEIEIDNDLKPLQFYSIEDGDRVLVRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG21</accession>
    <name>W5NG21_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Epoxide hydrolase 1, microsomal (xenobiotic)</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG21"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019580"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019612.1">
        <property type="protein sequence ID" value="ENSLOCP00000019580.1"/>
        <property type="gene ID" value="ENSLOCG00000015901.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002210"/>
    <dbReference type="InParanoid" id="W5NG21"/>
    <dbReference type="OMA" id="MEMPSML"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015901">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19709">
        <property type="entry name" value="bHLH_TS_TCF23_OUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF70">
        <property type="entry name" value="EPOXIDE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="467"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="558" mass="63363" checksum="10AB34A70A61DE80" modified="2014-04-16" version="1">MLLEVAAAVALGVVVCLLVFRKKKVEETLEAEDGWWGAGQRPESAEDETIRPFRVQTSTEEIQDLYRRIDQTRPVPSLEESRFHYGFNSTYLQKVVSYWRNEFDWEKQVAKLNKYPHFKTKIDGLDIHFIHVRPPHLPEGKTAIPLLMVHGWPSSFFEFYGILPLLTEPANPDDIVFQVICPSIPGYGFSEAPHKQGFDSVCAAHIFYKLMKRLGFNEFYAQGGDWGCLICTNIAQLQPSRVKGLHLSFLSVANPGLIAGLSLLFGQRFPGLFELKDHDVKRLFPFMKKVVVELLKESGYLHIQATKPDTAGRALNDSPVGLAVYILEKFSTWTDPEFRNLEDGGLERKYSIDDLLTNVMIYWVTGSIISSMRLYKEILGRGVGTQKHDKVPVRVPTAVACFPNELVHLPKIWAKQKYPNVVSFNYLPRGGHFGAFEEPELLAADIQSFVKKVQHRFEPQEKLSRPQHSPENAARERSRVRNLRQAFHSLQAALPAVPPDTKLSKLDILVLATNYIAHLTQTLDQGRIPTEQHLPPRAQGYLHPVKLQSLQLGMLQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGY9</accession>
    <name>W5NGY9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="7">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (R)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21260"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58773"/>
            <dbReference type="EC" id="1.8.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01008877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGY9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019898"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019931.1">
        <property type="protein sequence ID" value="ENSLOCP00000019898.1"/>
        <property type="gene ID" value="ENSLOCG00000016150.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0856">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155240"/>
    <dbReference type="HOGENOM" id="CLU_031040_8_4_1"/>
    <dbReference type="InParanoid" id="W5NGY9"/>
    <dbReference type="OMA" id="ITQVDDF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016150">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000004">
        <property type="entry name" value="Peptide-methionine (R)-S-oxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01400">
        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF56">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="9">
        <location>
            <begin position="56"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="21574" checksum="22C3DAE5CED4CD09" modified="2014-04-16" version="1">LQSPVASGFLGGNTAVMSAFSLLHLVTKSQPVALRPCGLPSGSCRDKKTYKVVFSQEELRKRLTPLQYHVTQERGTESAFRGKFTDHKEEGTYTCVVCGTPLFKSTTKFDSGSGWPSFFDLITPEAVCLTDDFSYGMHRVETSCSQCGAHLGHLFDDGPRPTGKRYCINSASLAFLLEAQASGTSGTSASGEGAGKEEGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH30</accession>
    <name>W5NH30_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Potassium voltage-gated channel subfamily Q member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218654.1">
        <property type="nucleotide sequence ID" value="XM_015363168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH30"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019939"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019972.1">
        <property type="protein sequence ID" value="ENSLOCP00000019939.1"/>
        <property type="gene ID" value="ENSLOCG00000016173.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691302"/>
    <dbReference type="CTD" id="556841"/>
    <dbReference type="eggNOG" id="KOG1419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155933"/>
    <dbReference type="HOGENOM" id="CLU_011722_10_1_1"/>
    <dbReference type="InParanoid" id="W5NH30"/>
    <dbReference type="OMA" id="HKQKLFR"/>
    <dbReference type="OrthoDB" id="8879391at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016173">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF8">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="116"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="17">
        <location>
            <begin position="436"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="17">
        <location>
            <begin position="596"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="392"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="922"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="468"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="932"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="954" mass="105464" checksum="3553546378FEB407" modified="2014-04-16" version="1">MPRNHSGDEGGSGLWMKTSPGHRTEVYDMKDVESGRRMKNSARVGDGLLPLGTGSSPSATGVGGPESQTGKQGARLSLLGKPLSYSSNQSCRRNVRYRRFQNYLYNVLERPRGWAFIYHAFVFILVFGCLVLSVLSTIPVHQDLSSRYLLILEFVMIVVFGLEYIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDIIVLIASVAVVSAGSQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFLVLIFSSFLVYLVEKEANPQFATYADALWWGTITLTTIGYGDKTPHTWTGRLLSAGFALLGISFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAASLIQCVWRSYAADENSVSIATWKPHLKALHTCSPTKKDQGESASSQKLSFKERVRMASPRGQSMKYRQTSVGDRRSPGPDITTDTSPAKVQKSWSFNDRTRFRPSLRLKSQSRTTPDADTNLGTDDGFDEKGCHCDVSVEDLTPALKTVIRAVRIMKFHVAKRKFKETLRPYDVKDVIEQYSAGHLDMLCRIKSLQTRLDTIVGPQQQLATKVKTFSSPSLHLYYSQHGKKQSTPGVDQILGKGQIPADKKTKEKVLPESEANEDLSMLGRVCKVEKQVQSIETKLDSLLDIYRQVLSKGSSSALTLASLPLFELDQTSDYQSSIASKDASSGSAQISRCVSRSTSANMPRGLQLILTPNELNNAPYYSFSPTMHSLPTQVPMSQNEGTVPILTPNNLSSQLAEMSKPAAPTSLQIPSLLPLKQINRPVSMIPVTTQESAGDVTACLVASKESRQVTKPNAKEEGSWRRHLSLETEPLVSVPTATDRGLGKSLSVQNLMQSTDELNTRLSSSDSNGSQVSQEMCSDWKEPRLFITDKDLDAGAESESFDLLSQPTVETVFSEDFTRTGIGGSSQSISKTGDSSESLSLPHVRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIF6</accession>
    <name>W5NIF6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kiaa0930</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033513">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633703.2">
        <property type="nucleotide sequence ID" value="XM_006633640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIF6"/>
    <dbReference type="FunCoup" id="W5NIF6">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020415"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020450.1">
        <property type="protein sequence ID" value="ENSLOCP00000020415.1"/>
        <property type="gene ID" value="ENSLOCG00000016527.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688586"/>
    <dbReference type="KEGG" id="loc:102688586"/>
    <dbReference type="CTD" id="23313"/>
    <dbReference type="eggNOG" id="KOG2465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004190"/>
    <dbReference type="HOGENOM" id="CLU_010018_1_0_1"/>
    <dbReference type="InParanoid" id="W5NIF6"/>
    <dbReference type="OMA" id="SAATHCH"/>
    <dbReference type="OrthoDB" id="1906921at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016527">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019141">
        <property type="entry name" value="DUF2045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477:SF13">
        <property type="entry name" value="KIAA0930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477">
        <property type="entry name" value="ZGC:172139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09741">
        <property type="entry name" value="DUF2045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="423" mass="48668" checksum="41EC7F881C2C6E06" modified="2014-04-16" version="1">MASFKSYCKTEPEEENGELDQSLQQMLKAIADERYRLNIRQEISGLGCLKDDRIVFWTWMFSNYFMEKWAPRQDDMLFYVRRKLSYVSADNTDGKKVEVEVYRRDSKKLPGLGDPDIDWEESVYLNLILQKLDYVVTCAVCTRSDGGDIHVHKKKSQQVFASPSKHPMDSKGEESKMSYPNIFFMIDNFEEVFSDMTVGEGEMVCVELVASDKSNTFQGVIFQGSIRYEALKKVYDNRVSVAAKMAQRMSFGFYKYNNMEFVRMKGPQGKGHAEMAVSRVPTGDTSPCGTEEDSNPDSPVHERVTSFSTPPTPERNNRPSFFSPSLRRKVPRNRIAEMKKSHSANDSEEFFRENEDNGDLHNTTNLRSRSLSGTGRSLVGSWLKLNRSEENFLLYSHLTYVTLPLYRITTDILEVRQKPILMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ07</accession>
    <name>W5NJ07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ07"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020616"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020651.1">
        <property type="protein sequence ID" value="ENSLOCP00000020616.1"/>
        <property type="gene ID" value="ENSLOCG00000016687.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2528">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156557"/>
    <dbReference type="InParanoid" id="W5NJ07"/>
    <dbReference type="OMA" id="FDSAPMR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016687">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07285">
        <property type="entry name" value="PX_SNX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000004">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014536">
        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037426">
        <property type="entry name" value="SNX9_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF2">
        <property type="entry name" value="SORTING NEXIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
        <property type="entry name" value="BAR_3_WASP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="276"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="69214" checksum="CFD372D3AE5E4259" modified="2014-04-16" version="1">MASKALVLYDFTAEPGNNELTVKEGETITITNQTIGGGWVEAQNSKGEVGLVPEDYIEVYIPNHYFESFPISLKVTKPSSGGNAVPDFSIFDTFSSTTTNTTSNQVDLGGCTAQPDTPETPVPAAASPNEANDSSDPWSTWNAGTTGNSTNNWGGKTESTEAATPNPAGNWNSTAHGHPQAYQGPGATDDDEWDDDWDELKSSGGFAESEAGDGGAMNRAGAHGSSMKIALNKFPGFSKSGPELYLLSKQPVKTKEKIPIYLGEVGPVWLYPETQLDCVVADPKKGSKMYGLKSYIEYQVTPSTTNRPVNHRYKHFDWLYERLLDKFGSAIPIPSLPDKQVTGRFEEEFIKMRMERLQGWMTRMCRHPVISYSEVFQLFLSFKDEKDWKTGKRKAEKDETVGVMIFTTVEPEAPDLDLIEVEQKCELFSRFTKAMDDGVKDLLNVGQEHWKRCTGPLPKEYQKIGKALQNLSSVFNTSGYQGESTLTDALTEAGKTYEEIAALVAEQPKKDLHFLMETNNEYKGLLGCFPDTIGVHKAAIEKVKEADKMVAASKITQQEKQTMAKRVSTMSYALQAEMNHFHSNRIYDYNRVMQLYLEQQVKFYEMIAEKLRQALNQFTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ92</accession>
    <name>W5NJ92_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carnitine O-palmitoyltransferase 1, muscle isoform</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carnitine O-palmitoyltransferase I, muscle isoform</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Carnitine palmitoyltransferase 1B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01023654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ92"/>
    <dbReference type="FunCoup" id="W5NJ92">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020701"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020737.1">
        <property type="protein sequence ID" value="ENSLOCP00000020701.1"/>
        <property type="gene ID" value="ENSLOCG00000016744.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286917"/>
    <dbReference type="HOGENOM" id="CLU_013513_2_1_1"/>
    <dbReference type="InParanoid" id="W5NJ92"/>
    <dbReference type="OMA" id="PERCGPY"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016744">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061959">
        <property type="term" value="P:response to (R)-carnitine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF69">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="24">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="23">
        <location>
            <begin position="176"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="791" mass="90864" checksum="4A9C804B810D06F5" modified="2014-04-16" version="1">MAEAHQAVAFQFTVTPDGIDFQLSREVLKNIYVSGVTSWRKRFIRFKNGILTGVYPASPSSWLIVVIAIMSTMYARIDPSVGMIDGIKKTLPVRDYLTTQTQTVLSAMLFATGLWLTLILILRYILKVLLSYHGWIFEPHGKMSVTTKIWAAQNLVKLFSGRRPLLYSFQASLPRLPVPSIDATIARYLESVRPLLDDEQYKQMESVANEFRKDPAPKLQKYLILKSWWATNYVSDWWEEYIYLRSRSPIMVNSNYYVMDLLYITPTHRQAARAGNVVHAMLMYRRKLDRGEHAPLRALDIVPMCSYQMERIFNTTRIPGVDTDYVQHLKESKHMVVYHKGRFFKVWLYYGGRHLQPSELEMQFQKILNDKTEPQPGELKVAALTAGERIPWATARATFFSQGVNKTSLDTIEKAAIFLTLDDEAHGYDQEKLRSLDLYAKSLLHGKCYDRWFDKSFTLIAYKNGKLGANAEHSWADAPIIGHLWEYLLSTDCFHLGYTEEGHCKGDTNKNLPPPSMLQWDIPQECQDIIESSYNIAKTIADDVDFHGCIFDDFGKGLIKKCRTSPDAFTQIALQLAHFRDKGEFCLTYEASMTRMFREGRTETVRSCTNESTAFVRAMEDPHRTGEEKLALFRRAAEKHQLMYRLAMTGAGIDRHLFCLYVVSKYLGIDSPFLKQVLSEPWRLSTSQTPQQQQHLVDIQKFPNHISAGGGFGPVADDGYGVSYNFVGENLITFHISSKFSSPETDSYRFGQNIRQAMLDIKALFHLKDKKINLTHAKKRHLSYKSNKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJL1</accession>
    <name>W5NJL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023594">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023596">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023598">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633808.1">
        <property type="nucleotide sequence ID" value="XM_006633745.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJL1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020856.1">
        <property type="protein sequence ID" value="ENSLOCP00000020820.1"/>
        <property type="gene ID" value="ENSLOCG00000016843.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692212"/>
    <dbReference type="KEGG" id="loc:102692212"/>
    <dbReference type="CTD" id="441631"/>
    <dbReference type="GeneTree" id="ENSGT00940000161249"/>
    <dbReference type="HOGENOM" id="CLU_055524_5_0_1"/>
    <dbReference type="OrthoDB" id="438211at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016843">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03155">
        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000005">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF198">
        <property type="entry name" value="TETRASPANIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="254" mass="28532" checksum="E23F660140CABFAD" modified="2014-04-16" version="1">MSRHYKDEQDDWITVCLKYLLFVFNFLFWVGGAAVMAVGIWTLIDKSDYLSLLASSTFAVSAYILIFAGGLVMITGFLGCCAVIREQRSCLSMYFSCLLLIFLIELVAGLLAYVYYQRLSDELKQHLKQTMTENYAQPGKDLITHAVDRLQQDFKCCGSNNASDWQYSVYISSPEAENRVVPDSCCKTITPHCGLRAHPSNIYKVEGGCITKLEQFLADHLLIIGAVGIGVACLQLCGMIFTCCLYRRIKLDPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK44</accession>
    <name>W5NK44_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Monocarboxylate transporter 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 16 member 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Sodium- and proton-independent thyroid hormones and aromatic acids transporter. Mediates both uptake and efflux of 3,5,3'-triiodothyronine (T3) and 3,5,3',5'-tetraiodothyronine (T4) with high affinity, suggesting a role in the homeostasis of thyroid hormone levels. Responsible for low affinity bidirectional transport of the aromatic amino acids, such as phenylalanine, tyrosine, tryptophan and L-3,4-dihydroxyphenylalanine (L-dopa). Plays an important role in homeostasis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3,3',5-triiodo-L-thyronine(out) = 3,3',5-triiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71811"/>
            <dbReference type="ChEBI" id="CHEBI:533015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:71812"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:71813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-phenylalanine(in) = L-phenylalanine(out)</text>
            <dbReference type="Rhea" id="RHEA:27950"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:27951"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:27952"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-thyroxine(out) = L-thyroxine(in)</text>
            <dbReference type="Rhea" id="RHEA:71819"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:71820"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:71821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tryptophan(in) = L-tryptophan(out)</text>
            <dbReference type="Rhea" id="RHEA:70947"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:70948"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:70949"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosine(in) = L-tyrosine(out)</text>
            <dbReference type="Rhea" id="RHEA:68572"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:68573"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:68574"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012042">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK44"/>
    <dbReference type="FunCoup" id="W5NK44">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021003"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021039.1">
        <property type="protein sequence ID" value="ENSLOCP00000021003.1"/>
        <property type="gene ID" value="ENSLOCG00000016991.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157966"/>
    <dbReference type="HOGENOM" id="CLU_001265_59_1_1"/>
    <dbReference type="InParanoid" id="W5NK44"/>
    <dbReference type="OMA" id="LSYRIWA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016991">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015192">
        <property type="term" value="F:L-phenylalanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015196">
        <property type="term" value="F:L-tryptophan transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070327">
        <property type="term" value="P:thyroid hormone transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001016">
        <property type="entry name" value="Solute carrier family 16 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF119">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="17">
        <location>
            <begin position="272"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="466"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="503" mass="53427" checksum="C5DB89405D31002D" modified="2014-04-16" version="1">MTEGTDPERAAAAGTSTALPAEGPARKAEDQPLDEDGSRENGGSRGTPPGSAGGADGAAEPEFVHPEGGWGWVVMLAAMWCNGSEFGTGADSGACTPPCVSQDEDLKFKTSWVGSLSMGMIFFCSPVVSIFTDLFGCRMTAVAGAAVGLVGLFASSFVSSLGPMYFTYGIVFACGCSFAYQPSLVILGHYFKKRLGLVNGMVTAGSSVFTIALPFLMSQLLQRLGLHHTLRVLCVFMLLLLLASLTYKPLLPQPGRSKSGGRCPPMSKVFNVKIWKSLGYRIWAFGIPAALYGYFVPYVHLMKHVEEKFGKDANKEVLLMCIGITSGVGRLIFGRVADYVPGVNKVYLQVSSFVVIGLMSMMIPLCDIFGGLIAVCLLMGLFDGCFICIMAPIAFELVGSQNVSQAIGFLLGMMSVPMTVGPPIAGFLRDRLGSYDVAFYLAGIPPLIGGAVLCFIPWAERRKRRQQQEAAGDTAEKMLGAKPNGAFPVEGAGGEKTDHESAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKJ4</accession>
    <name>W5NKJ4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Opioid growth factor receptor-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218701.1">
        <property type="nucleotide sequence ID" value="XM_015363215.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKJ4"/>
    <dbReference type="FunCoup" id="W5NKJ4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021153"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021189.1">
        <property type="protein sequence ID" value="ENSLOCP00000021153.1"/>
        <property type="gene ID" value="ENSLOCG00000017116.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690760"/>
    <dbReference type="KEGG" id="loc:102690760"/>
    <dbReference type="CTD" id="79627"/>
    <dbReference type="eggNOG" id="ENOG502RA9J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018730"/>
    <dbReference type="InParanoid" id="W5NKJ4"/>
    <dbReference type="OMA" id="IDPISKH"/>
    <dbReference type="OrthoDB" id="9030204at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017116">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140625">
        <property type="term" value="F:opioid growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006757">
        <property type="entry name" value="OGF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039574">
        <property type="entry name" value="OGFr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015:SF0">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04664">
        <property type="entry name" value="OGFr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opioid growth factor receptor (OGFr) conserved" evidence="3">
        <location>
            <begin position="98"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="426" mass="49541" checksum="0777FEF6B58275A9" modified="2014-04-16" version="1">MGNLMGSLRYKEPSTVEECDSTWETDSDPEELQPVDDNNMTAEDRLANSSRCGDEENLTSHQFTDARESATKLKRSYYAAKDLYKYRHSYPNYKKPRQPSEFRNLRFYLNKIPLVPDGIFVEEILNKWKGDYEKLEHNHTYIQWLFPLREQGLNFHAQELAQDEIKEFQNTREAKRRFLLAYTVMLDFFGIKLLDKNGNVARAANWQDRFQHLNESQHNYLRITRILKSLGELGFESFKPPLVKLLLEEALVEETIPNMKHSALEYFVYTIRDRKERRTLLRFAQLYYRPADQFIWGPPMKRNPGGRACSTQDKSAVQSKPVKGASASSPSSQSNSDKAEDNRSDKTEDRNQRIAEIQGSTDGPLSITEETPVSEEDRDSQEGKVAAENRAKQSNSCKIKPQFEGKVENKIRKDRNEYIDPISKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL33</accession>
    <name>W5NL33_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL33"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021342"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021379.1">
        <property type="protein sequence ID" value="ENSLOCP00000021342.1"/>
        <property type="gene ID" value="ENSLOCG00000017254.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW7E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="HOGENOM" id="CLU_037388_0_0_1"/>
    <dbReference type="InParanoid" id="W5NL33"/>
    <dbReference type="OMA" id="EMFYLMC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017254">
        <property type="expression patterns" value="Expressed in liver and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF105">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="157"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="34974" checksum="DAAA2181439A9FD3" modified="2014-04-16" version="1">MGDWTFLGRLLDKVQSHSTVVGKIWLTVLFVFRILVLGAGAEKVWGDEQSDFLCNTQQPGCENVCYDKAFPISHIRFWVLQIIFVSTPTLVYLGHVLHVIHKEEKLRQRKLKEEQDGNLLMKKISKIPKYTDADGKVRIQGNLLCSYLINVIFKILFEVGFIVGQYYLYGFVLDPVFVCNRSPCPFKVECYMSRPTEKTIFILFMLVVACISLLLNVIEIFYLCGRKISYAVNKQYQQQIGGLKIPLTSEVVSSGFKAPLDCETFSDKHSVLQNKTNLKIEQNEDKLESEMTRSIIKKHIITNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLH5</accession>
    <name>W5NLH5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLH5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021484"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021521.1">
        <property type="protein sequence ID" value="ENSLOCP00000021484.1"/>
        <property type="gene ID" value="ENSLOCG00000017379.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="HOGENOM" id="CLU_005389_4_0_1"/>
    <dbReference type="InParanoid" id="W5NLH5"/>
    <dbReference type="OMA" id="NSERTHC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="250" mass="28052" checksum="701538AC0238EA3B" modified="2014-04-16" version="1">VAIILFHYKHTPIVKANNSELSFLLLFSLTLCFLCSLTFIGQPSDWSCMLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGNNIMKWFGPKQQRLSVLGFTLVQVLICILWVTISPPFPNKNIKYYKDRIILECDLGSAGAFWAALGYIGFLSALCFVLAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYISSPGKFTVAVEIFAILASSFGLLFCIFAPKCYIILLQPEKNTKKHLMGKMPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMA5</accession>
    <name>W5NMA5_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NMA5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021801.1">
        <property type="protein sequence ID" value="ENSLOCP00000021764.1"/>
        <property type="gene ID" value="ENSLOCG00000017659.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170246"/>
    <dbReference type="HOGENOM" id="CLU_000680_32_7_1"/>
    <dbReference type="InParanoid" id="W5NMA5"/>
    <dbReference type="OMA" id="MKLANVR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46238:SF8">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46238">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="131" mass="15434" checksum="4CD94556AD2B2A9C" modified="2014-04-16" version="1">ALYGTECWPTTKKHEQALHTMEMKMLRWNLGLTRLDHVMNEGVQKTLKVTPITEKMRELCVRWYGNVLRSNDTSTAKTVFELNVEGCRPRGRPFTRWLDRLKDDMRLAKVTCRDAADHTKWKNRCKQVDPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMZ0</accession>
    <name>W5NMZ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMZ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021999"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022038.1">
        <property type="protein sequence ID" value="ENSLOCP00000021999.1"/>
        <property type="gene ID" value="ENSLOCG00000017896.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169031"/>
    <dbReference type="HOGENOM" id="CLU_174440_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMZ0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017896">
        <property type="expression patterns" value="Expressed in muscle tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="109" mass="11911" checksum="2248D569A328B7D9" modified="2014-04-16" version="1">LQVDPQALLPAELCGVAVPPGSKALEYSNGIFDCQSPTSPFMGSLRALQLIEDLRGLLELMETEEKEGLRCQIPDSTAELIVEWLQGHMVGPGCGRARFKNSLIPVVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNB2</accession>
    <name>W5NNB2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Trace amine-associated receptor 13c-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNB2"/>
    <dbReference type="FunCoup" id="W5NNB2">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000022162.1">
        <property type="protein sequence ID" value="ENSLOCP00000022121.1"/>
        <property type="gene ID" value="ENSLOCG00000018020.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271932"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_0_1"/>
    <dbReference type="InParanoid" id="W5NNB2"/>
    <dbReference type="OMA" id="CIASIDG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="343" mass="38736" checksum="B166CE388A5C2787" modified="2025-10-08" version="2">MELHELQEFKTLNYCYQQYNLSCSKEVRSPAVYVVMYISAVAVVMLTVCGNLVVIISISHFKQLHTPTNLLLLSLTVADFLVGVMVMPFSLIELIETCWYFGETFCTLYNIFVFVLTSVSISNLVFVAIDRYFAVCDPLLYSTKITIKIPCLFILLSWLVSLLYNVALIYCNENIGRSAKLNACLGDCLLSVNKNWGTADLLITFVVPCSIMLTLYLKIFIVARKHAQAIRCAADQIHSTTGVQRTIPKKSERKAAKTLGIVVLGFLLCWVPYYICIIVEANMNLSSSVLMNFLSFLVYFNSSVNPVIYGLFYPWFQKSLKLIVTFKICSPASSLINLFPEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNH8</accession>
    <name>W5NNH8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNH8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022187"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022228.1">
        <property type="protein sequence ID" value="ENSLOCP00000022187.1"/>
        <property type="gene ID" value="ENSLOCG00000018086.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZ9M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166084"/>
    <dbReference type="HOGENOM" id="CLU_033666_0_6_1"/>
    <dbReference type="InParanoid" id="W5NNH8"/>
    <dbReference type="OMA" id="MQEYKDH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018086">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="141"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37744" checksum="460C9E8EC7DD5EB4" modified="2014-04-16" version="1">AFDISPFTRGRVVGQSDCGHSERQIARSLGLANGTVHNIIVKYRQSNERAVNHRPGRPSMLTEGTKRALVRVVSANPRTKLVDIASTVNLSQRTAQRYLHKLGYNGSLSRPKPLLTKANVKRRLAWAQEMKEKPAAFWRSVVFTDQSQFQQFASSGRTWAWRKAGQAFQEDCVQPPAQDDGFSVMVWAAVWHAGKTELVFTEGPLDSPGYVSVLENHLLPLFRQGALQFSTHLLQEDCAPCHTSKTTTHWKSERGLQVVPWPPRSPDMNPIAHIWERLDRQLQQTTPRPPSAAALRDRLSELWRGLDQALVQTLIAGLQQRVLALHEARGRATRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNL3</accession>
    <name>W5NNL3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Threonine synthase like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNL3"/>
    <dbReference type="FunCoup" id="W5NNL3">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022222"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022263.1">
        <property type="protein sequence ID" value="ENSLOCP00000022222.1"/>
        <property type="gene ID" value="ENSLOCG00000018121.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685537"/>
    <dbReference type="KEGG" id="loc:102685537"/>
    <dbReference type="eggNOG" id="KOG2616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161144"/>
    <dbReference type="HOGENOM" id="CLU_015170_3_2_1"/>
    <dbReference type="InParanoid" id="W5NNL3"/>
    <dbReference type="OMA" id="HFNRCQH"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018121">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="1">
        <location>
            <begin position="251"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="748" mass="82190" checksum="7CB025FBEF8ACCCA" modified="2014-04-16" version="1">MFHPSCRLVKNIAIKGLLAYHRGRASNESNSRLLTRKTFFSPIVSFSASRTASLSSCTGHKNIFIMGLPGSGKTTVGNILGSKLGHPVIDLDDHILKKEWNMTAGEKLSEIGWEQFVEEEGKALSSFSASGSIVLLTESSPMHSAAMHHVKQNGIVIYLDVNPEVIIKRLSGGRGNRIVGRGSGVSLREILRCRRKIYNKWCDARVLCDSGDSAEEVAANVLKAVKRCEDTESEVFVSTRNKFLPEDGSVSERKFFSDFLVEGLAPDGGLYVPQGGLSELPPREWVRLVDLPYSERAQILLEKCIHPADVPAPELYSMVKKAYADNFACPRIAPVRHLVHNQYVQELFHGPTASFRDFSLLLLPQLLAYCTSNLCNHLLLGATSGDTGSAALNGFSRLPEAEKRRSAVLVFFPEAGVSPVQRLQMTGFREGNLKAVGVQSDLAFCQQAVRQMLGDPKLTGHLAVECGAVLTAADSVNWAQLLPQVIFHASAYLDLVSQGTVAFGEPVDVCIPAGDFGNILSAVYAKQMGIPIRKFICASHQSGVLTKFMRTGEYDLRDRQLLPSSSPATEVLKPSSLERYLFHISDGDWQLVRGLFSQFERERYFKVPRALLKRMHQDCLAGWCSEEECLAAISAVHSATGYILDTRAAAAKVVADRLQDRTCPVIISSTVHPAKFAPAVLKALKFQDVAEDPFDQLNLLSSLGSLPPMHADLKSLRDPRRHKVCDANVSDLVEEVETVVRDSFLKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNP0</accession>
    <name>W5NNP0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Chemokine-like receptor 1 like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNP0"/>
    <dbReference type="FunCoup" id="W5NNP0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022249"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022290.1">
        <property type="protein sequence ID" value="ENSLOCP00000022249.1"/>
        <property type="gene ID" value="ENSLOCG00000018148.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696733"/>
    <dbReference type="KEGG" id="loc:102696733"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230438"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_0_1"/>
    <dbReference type="InParanoid" id="W5NNP0"/>
    <dbReference type="OMA" id="HIATKEW"/>
    <dbReference type="OrthoDB" id="6117944at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018148">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002430">
        <property type="term" value="P:complement receptor mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14974">
        <property type="entry name" value="7tmA_Anaphylatoxin_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF0">
        <property type="entry name" value="N-FORMYL PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
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            <begin position="57"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022249.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="350" mass="40403" checksum="7B96C6D144B63DE0" modified="2014-04-16" version="1">MDQTYDYDYNISSSDKSSYYNSTEKTYSKPYTMQQPSVMNIMIIVINSIIFILGVIGNGIVICITGFKMKRTINTTWFLSLAVSDFLFCVFLPFSIVQMASDQHWHFGKFMCKFTSFIMFLNMFSSIFLLVIISIDRSVSVFYPVWSQNYRTTRFATVVVLIAWIISALLSTPSLIFRDTAVKSEKTHCFNKYGSGMSKKNLHRVVALTRFVFGFVIPFIVIAFCYSIIIIKLKKNRMAKSLKPFKIMTAIIVTFFLCWLPYHIFIILEINHYNYSPDIFLKWMSMAVSLAFANSCLNPILYVFMGKDFKEKFKVSFLSKIENALSEDARTTTRRISRTSSAFEKGSTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWE8</accession>
    <name>W5LWE8_LEPOC</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name type="common">Spotted gar</name>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </reference>
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    <dbReference type="Proteomes" id="UP000018468">
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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        </location>
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            <end position="145"/>
        </location>
    </feature>
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            <begin position="12"/>
            <end position="42"/>
        </location>
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        <location>
            <begin position="48"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="145" mass="15711" checksum="604F479A97E9F092" modified="2014-04-16" version="1">MCVNCVLVCVNILPVCGCVCIYTACVLVCVNILPVCGCVYILPVGGCVYIYCLLVCVCVYCLLVCVCVYCLLVCVCIYCLLMGVCIYCLLVCVCVCVYCLLVCVCVYCLLVCVYILPVGVCVYTACWCVCVYCLLMGVYILPVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSLOCG00000000767.1"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:immunoglobulin mediated immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="22"/>
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    </feature>
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        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="146" mass="15877" checksum="B9AD6B088CA34E3B" modified="2014-04-16" version="1">MTAPLALGLLLSLATLSCVQSDVVLTQSESAVVTPGGSHRLKCTVSGFNVASYSMYWIRQAPGKGLEWIVYFYSSSDNSYAPAVQGRFTASKDSSSLYLDMTSLRAEDTAVYYCARDTVQQVACRVRQNLAEQTECGSVLCGLHFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXP1</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000898"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000902.1">
        <property type="protein sequence ID" value="ENSLOCP00000000898.1"/>
        <property type="gene ID" value="ENSLOCG00000000811.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SQQV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_5_2_1"/>
    <dbReference type="InParanoid" id="W5LXP1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000811">
        <property type="expression patterns" value="Expressed in bone element and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002397">
        <property type="entry name" value="Immunoglobulin heavy variable 4-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865268" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="119" mass="13626" checksum="D8B52796CA2A58AB" modified="2014-04-16" version="1">MRILPVLLLSVFFLKYVRSDVVFTQSGPEIKKPGESFKISCKASGYTYTDYNINWIRQSPVKGFEWLGYITSRYSTGYADAVKGKMTFTRDNSNSMAYLQMSNPTSEDTAVYYCARYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY48</accession>
    <name>W5LY48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EVA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LY48"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001055"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001059.1">
        <property type="protein sequence ID" value="ENSLOCP00000001055.1"/>
        <property type="gene ID" value="ENSLOCG00000000948.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154096"/>
    <dbReference type="HOGENOM" id="CLU_050537_0_1_1"/>
    <dbReference type="InParanoid" id="W5LY48"/>
    <dbReference type="OMA" id="HQLCPSR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000948">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22828">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22829">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000006">
        <property type="entry name" value="Protein eva-1 C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000003">
        <property type="entry name" value="Protein eva-1 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052461">
        <property type="entry name" value="EVA1_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039500">
        <property type="entry name" value="EVA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422:SF1">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14851">
        <property type="entry name" value="FAM176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0348">Hemagglutinin</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865282" description="SUEL-type lectin domain-containing protein" evidence="11">
        <location>
            <begin position="26"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
            <begin position="48"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
            <begin position="155"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="479" mass="54625" checksum="B19C6BF8CACF3344" modified="2014-04-16" version="1">IVCSFGTLPCISCLIKWISITTVYCDLFVQVDLCRYLTRALRNYTEQACDGEYFSVQCPPRTTISIQSSFYGRKVPNHQMCPPHLSPSNTKLFKEDTTCSVSTSLRKMLDECQDRRSCHVLVNSRLFGIDPCPSNSKYLIVWYKCRPNEYKNKVACEGDKMRLSCKRSMVIAIYSAFFGRSQEGTLECPYVNSRVPAVECQTGTALQIMTDRCHGKRICSVHATSREFGDPCYSGIHKYLNVIYTCVPKKLLEDIDSKRTVSHNPSFPEHPRLHDPNIIGDGVLPDGPTFVTTGSLPEQKKKRGRESETYSREEKPFLDAVDGDDRQVLNHTMESRSINTEMTLITNVLAAYTYISENPERAALYFVSGVCLGLILTLCALVVQISCRTDCKTTVPQKRQKKVESDSDSSDSDSDWDTTSDLSARRHRRFERTLNMNVFTSAEELERAQRLEERERIIREIWMNGQPDIPGTRSLNRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY88</accession>
    <name>W5LY88_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LY88"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001095"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001099.1">
        <property type="protein sequence ID" value="ENSLOCP00000001095.1"/>
        <property type="gene ID" value="ENSLOCG00000000977.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234535"/>
    <dbReference type="HOGENOM" id="CLU_048583_2_1_1"/>
    <dbReference type="InParanoid" id="W5LY88"/>
    <dbReference type="OMA" id="MAGLAWK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000977">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF3">
        <property type="entry name" value="ALPHA-N-ACETYLNEURAMINIDE ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="169" mass="19451" checksum="B7907C8E0722BC1D" modified="2014-04-16" version="1">NVSVAFFRFQNLLWSRKKFVESVKVYGSSYIYMPAFSMKPGTDPSFRAYHALSDIGANQTVVFANPDFLKNVGKFWKSRGIHAKRLSTGLFLVSLALGLCDEVTIYGFWPFSVDLNERFISHHYYDNILPFSGFHAMPEEFLQLWVLHKTGTLRMRVGQCEELRISPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYC2</accession>
    <name>W5LYC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aromatic-L-amino-acid decarboxylase</fullName>
            <ecNumber evidence="10">4.1.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DOPA decarboxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01012336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYC2"/>
    <dbReference type="FunCoup" id="W5LYC2">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001129"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001133.1">
        <property type="protein sequence ID" value="ENSLOCP00000001129.1"/>
        <property type="gene ID" value="ENSLOCG00000000998.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156004"/>
    <dbReference type="HOGENOM" id="CLU_011856_3_1_1"/>
    <dbReference type="InParanoid" id="W5LYC2"/>
    <dbReference type="OMA" id="NPGFNWS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000998">
        <property type="expression patterns" value="Expressed in intestine and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000018">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF167">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="481" mass="54126" checksum="57CC672AF806E28C" modified="2014-04-16" version="1">KMDPAEFRRRGKEMVDIISDYLENIEQRPVFPDVEPGYLRALVPQEAPEEPESFDEIVRDIERVIMPGVTHWHSPYFFAYFPTASSFPAMLADMLSGAIGCIGFSWAASPACTELETVMLDWLGKMLKLPEEFLAGTAGQGGGVIQGTASEATLIALLAARTRTVKKIQAENPGTDEEDILPKLVAYASDQAHSSVERAGLIGGVKMKMIPSDDKFAARGEALRQTIEADKAAGLIPFYFCATLGTTPSCAFDRISELGPICNSENIWMHIDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRADIVGAFKMDPLYLKHDHQESGLVTDYRHWQIPLGRRFRSLKMWFVFRMYGLKGLQQYIRKHVSLAKEFESLVRSDQRFEISAEVVLGLVCFRLKGSNALNEKLLKRINDTRKIHLVPCHLSGSFVLRFAVCARTTESKHIQFAWKHIAEIASQLLQEPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYY8</accession>
    <name>W5LYY8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYY8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001345"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001350.1">
        <property type="protein sequence ID" value="ENSLOCP00000001345.1"/>
        <property type="gene ID" value="ENSLOCG00000001196.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_1_2_1"/>
    <dbReference type="InParanoid" id="W5LYY8"/>
    <dbReference type="OMA" id="CARREPH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001196">
        <property type="expression patterns" value="Expressed in mesonephros and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002762">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="144"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="261" mass="28868" checksum="151444A464825B70" modified="2014-04-16" version="1">SITLTQPASAEVKKPGESLKLSCQVSGFQIDSNWKSWIRQAPGKGLEWLLNYHSGSSVEYSSTIQGHFTPSIDTSSSTFFLQMNNLRAEDTAVYYCARDTPVQIHRHKPGPENCQWVFGIKLTTRVSCVLCFLLLGLSAVTAQSSITLTQPASADLKKPGESLKLSCQVSGFSIGSYWKGWIRQASGKGLEWLALYYDESNTKYYASSVQGRFTASMDTSSSTFFLQMNNLRAEDTAVYYCARDTVTQLNQEAVQKPALST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZN8</accession>
    <name>W5LZN8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CKLF-like MARVEL transmembrane domain containing 8b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZN8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001595"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001600.1">
        <property type="protein sequence ID" value="ENSLOCP00000001595.1"/>
        <property type="gene ID" value="ENSLOCG00000001400.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160520"/>
    <dbReference type="HOGENOM" id="CLU_103581_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZN8"/>
    <dbReference type="OMA" id="VRGRHNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001400">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013295">
        <property type="entry name" value="MAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF10">
        <property type="entry name" value="CKLF-LIKE MARVEL TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01884">
        <property type="entry name" value="MALPROTEIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="40"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="179" mass="20016" checksum="85C6AD57F6C61A6C" modified="2014-04-16" version="1">KEAVEPAKMEDGPRSHTVITTSSSYLENFSTSTLAYDKQFIHTAPGILVFAEIVLGLLVWTLIAGTEYFRVPAFGWVMFVAVFYWVLTVFLLIIYLTRAHSRIPQVPWRTVGLCFNGSAFVLYFIAAVVDAVSVKAAPRSGYNFNSWAASSFFAFLVALCYGGNTYFSFRSWRAGDEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0T4</accession>
    <name>W5M0T4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peflin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">PEF protein with a long N-terminal hydrophobic domain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Penta-EF hand domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">COPII-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0T4"/>
    <dbReference type="FunCoup" id="W5M0T4">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001991"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001996.1">
        <property type="protein sequence ID" value="ENSLOCP00000001991.1"/>
        <property type="gene ID" value="ENSLOCG00000001726.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0037">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155722"/>
    <dbReference type="HOGENOM" id="CLU_051357_1_0_1"/>
    <dbReference type="InParanoid" id="W5M0T4"/>
    <dbReference type="OMA" id="YNKSYNP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001726">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048208">
        <property type="term" value="P:COPII vesicle coating"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16184">
        <property type="entry name" value="EFh_PEF_peflin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000139">
        <property type="entry name" value="Peflin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF10">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="216"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="116"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="319" mass="33304" checksum="1DBAA58601773968" modified="2014-04-16" version="1">MRTDVDGHDARTSSTGYQGPTGGGYFPGGQPPPGDPYRGGAPPGGPGGHYGGTAPPSGPGSQYGGSAPPPGGHYPGGPTPRAPYGSYGPGPGGQYGGGGGAAPGATYGGGAPAGGGQFGGAPGTPYGGYGQHHGGPYGQQAPAGNIPPGVDPEAFQWFQSVDTDHNGSITLKELKQALVNSNWSAFNDETCLMMINMFDKTKTGKIDVFGFSALWTFMQQWRALFQQFDRDRSGSINTNELHQALCQMGYNLSPQFAQSLVSRYGGREAQGAVQLDRFIQVCTQLQSMTQAFREKDTAMTGNVRLSYEDFLSSTIVRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M152</accession>
    <name>W5M152_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L23, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M152"/>
    <dbReference type="FunCoup" id="W5M152">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002109"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002114.1">
        <property type="protein sequence ID" value="ENSLOCP00000002109.1"/>
        <property type="gene ID" value="ENSLOCG00000001825.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684237"/>
    <dbReference type="KEGG" id="loc:102684237"/>
    <dbReference type="CTD" id="6150"/>
    <dbReference type="eggNOG" id="KOG4089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007739"/>
    <dbReference type="HOGENOM" id="CLU_103097_1_0_1"/>
    <dbReference type="InParanoid" id="W5M152"/>
    <dbReference type="OMA" id="PNFWLKL"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001825">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000284">
        <property type="entry name" value="39S ribosomal protein L23, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="153" mass="18196" checksum="DAC79A238DAE1101" modified="2014-04-16" version="1">MARRVIYPLYQIGNPQLRIFRTNFFMTLVRPGKEQPPDTVQFRIPMEMTKCDVKNYLEKIYSVPVGAVRTRIQYCTNKKRNHLNQRVKRPDYKVAYVQLAQQQTFQFPDIFPEKDKTAEPGSVEDFQDKFMEDEKQRQKADPRRGGVTEWFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1D3</accession>
    <name>W5M1D3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenosine deaminase</fullName>
            <ecNumber evidence="7">3.5.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Adenosine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2'-deoxyadenosine + H2O + H(+) = 2'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:28190"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:28191"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01021159">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639523.1">
        <property type="nucleotide sequence ID" value="XM_006639460.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1D3"/>
    <dbReference type="FunCoup" id="W5M1D3">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002190"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002196.1">
        <property type="protein sequence ID" value="ENSLOCP00000002190.1"/>
        <property type="gene ID" value="ENSLOCG00000001870.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689495"/>
    <dbReference type="KEGG" id="loc:102689495"/>
    <dbReference type="CTD" id="100"/>
    <dbReference type="eggNOG" id="KOG1097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183113"/>
    <dbReference type="HOGENOM" id="CLU_039228_0_1_1"/>
    <dbReference type="InParanoid" id="W5M1D3"/>
    <dbReference type="OMA" id="NHFTIHA"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001870">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060205">
        <property type="term" value="C:cytoplasmic vesicle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060169">
        <property type="term" value="P:negative regulation of adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01320">
        <property type="entry name" value="ADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000038">
        <property type="entry name" value="Adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00540">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028893">
        <property type="entry name" value="A_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF43">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="22">
        <location>
            <begin position="14"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="41137" checksum="08E4DA859C02D1DB" modified="2014-04-16" version="1">MAESNSRQAVFDKPKVELHVHLDGAIRLETILDVAKRRNIPLPAQNAKNLQKMFTMEEPGTLTEFLKKFDIYMGVIAGDREAIKRIAFEFVETKAKEGVIYVETRYSPHFLANCMVNPISWNQTPGDVTPDDVVNLVNQGLREGEQAFNIKARSILCCMRHMPSWSNEVVELCKKYRGDGVVAIDLAGDESMDCISNQAHREAFEEAVRSDIHRTVHAGEVGPASVVKEAVEVLKAERIGHGYHTIDDPVLYRQLLKQDMHFEVCPVSSRLTGGCPADFTKHPLLRFKKDKANYSLNTDDPLIFNSTLNSDYKVVTKYMGFTEEEFKRVNLKSAQSSFLPEKEKKDLLNHLHEAYGMVESSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1J7</accession>
    <name>W5M1J7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gamma-glutamylcyclotransferase b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1J7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002255"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002260.1">
        <property type="protein sequence ID" value="ENSLOCP00000002255.1"/>
        <property type="gene ID" value="ENSLOCG00000001951.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044921"/>
    <dbReference type="HOGENOM" id="CLU_048475_2_2_1"/>
    <dbReference type="InParanoid" id="W5M1J7"/>
    <dbReference type="OMA" id="YFHYPVE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001951">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935:SF14">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13772">
        <property type="entry name" value="AIG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="177" mass="20047" checksum="6D3CCB527A31F048" modified="2014-04-16" version="1">KMEDETFLYFAYGSNLLKERLQLKNPSATIHCVARLKDYKLAFGNFGGRTSDRWHGGVATIVQSPGDEVWGVWKMSVEDLQSLDRQENVKIGAYSPAEVMVSTKVEELTCRTYIMNNCVYTPPSPQYLKVIVLGAEQNSLPSEYQLKLKAIKTNNYEGPLDLMSEVEKAMKIKNKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M299</accession>
    <name>W5M299_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M299"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002507"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002513.1">
        <property type="protein sequence ID" value="ENSLOCP00000002507.1"/>
        <property type="gene ID" value="ENSLOCG00000002153.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00920000149457"/>
    <dbReference type="HOGENOM" id="CLU_087312_0_0_1"/>
    <dbReference type="InParanoid" id="W5M299"/>
    <dbReference type="OMA" id="EGLCGVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002153">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000019">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF23">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="29"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21212" checksum="C7D632F9E0ADF4FA" modified="2014-04-16" version="1">MSEPAGKRLKLTLPVCTAHRTLTGTRVKHNFPLVVEESLCGVITVLMAVAYRLQALAKLFERDDIALPRIAAFFHQEAEQEQKEAESLLEYLRERGGQYCNKDIQKPGCEAVGSVLQALELMLAQWKEVARILTELCQQSKQSGDPHTASVVKKRFVTPAIGKIEVVGCKDETGFGEYLIDQLQKELTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3W8</accession>
    <name>W5M3W8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G protein-activated inward rectifier potassium channel 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cardiac inward rectifier</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Heart KATP channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Inward rectifier K(+) channel Kir3.4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">KATP-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Potassium channel, inwardly rectifying subfamily J member 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642286.2">
        <property type="nucleotide sequence ID" value="XM_006642223.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3W8"/>
    <dbReference type="FunCoup" id="W5M3W8">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003076"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003080.1">
        <property type="protein sequence ID" value="ENSLOCP00000003076.1"/>
        <property type="gene ID" value="ENSLOCG00000002615.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687729"/>
    <dbReference type="KEGG" id="loc:102687729"/>
    <dbReference type="CTD" id="3762"/>
    <dbReference type="eggNOG" id="KOG3827">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257365"/>
    <dbReference type="HOGENOM" id="CLU_022738_11_0_1"/>
    <dbReference type="InParanoid" id="W5M3W8"/>
    <dbReference type="OMA" id="SHEINAK"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002615">
        <property type="expression patterns" value="Expressed in heart and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000019">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000005">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003277">
        <property type="entry name" value="K_chnl_inward-rec_Kir3.4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF52">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01330">
        <property type="entry name" value="KIR34CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="8 20" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="23">
        <location>
            <begin position="58"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="24">
        <location>
            <begin position="204"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="391"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="451" mass="51180" checksum="025E3DAB830141AF" modified="2014-04-16" version="1">MKMAGDLRVHMDHDMEIGVAPREVKKLPRRAREGLAHVSTDRTHLIAEAVKKPRQRYVQKDGKCNVHHGNVQETYRYFSDLFTTLVDLKWRFSLFIFTLVYCVTWLFFGFIWWLIAYIRGDLEHGDEQGWTPCVENLNSFVSAFLFSIETETTIGYGYRVITENCPEGIILLLVQAILGSIVNALMVGCMFVKISQPKKRAETLMFSHKAVISVRDDKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEINEKSPFWELSKAQMEKEEFEIVVILEGMVEATGMTCQARSSYQDTEVLWGYRFTPVLTLEKGFYEVDYNTFHDIYETPTPSCSARELATLVREGQLLPQLCLPPLEPTPTLALGPGQQEENGRVEREPAEDWEGGEAHRPTGRGGLCVAMETGESVCCPSPRPNTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4N5</accession>
    <name>W5M4N5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhombotin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LIM domain only protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4N5"/>
    <dbReference type="FunCoup" id="W5M4N5">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003343"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003350.1">
        <property type="protein sequence ID" value="ENSLOCP00000003343.1"/>
        <property type="gene ID" value="ENSLOCG00000002844.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160199"/>
    <dbReference type="HOGENOM" id="CLU_001357_3_1_1"/>
    <dbReference type="InParanoid" id="W5M4N5"/>
    <dbReference type="OMA" id="MCGGCQQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002844">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043534">
        <property type="term" value="P:blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061304">
        <property type="term" value="P:retinal blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09384">
        <property type="entry name" value="LIM1_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09385">
        <property type="entry name" value="LIM2_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000059">
        <property type="entry name" value="LIM domain only 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000016">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF3">
        <property type="entry name" value="RHOMBOTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="92"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="161" mass="18674" checksum="A2C2942B563BBDD4" modified="2014-04-16" version="1">MSSAIERKSLEASEEPVDEVLQMPPSLLTCGGCQQSIGDRFFLKAIEQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAFEMTMRVRDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIFEWTKLNNMVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5B7</accession>
    <name>W5M5B7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase</fullName>
            <ecNumber evidence="24">2.4.1.135</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 24">
            <text>3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24168"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="EC" id="2.4.1.135"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="24">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer. Interacts with SAR1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 24">Golgi apparatus membrane</location>
            <topology evidence="2 24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 24">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.135" evidence="24"/>
    <dbReference type="EMBL" id="AHAT01024152">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642230.1">
        <property type="nucleotide sequence ID" value="XM_006642167.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5B7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003582.1">
        <property type="protein sequence ID" value="ENSLOCP00000003575.1"/>
        <property type="gene ID" value="ENSLOCG00000003017.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692986"/>
    <dbReference type="KEGG" id="loc:102692986"/>
    <dbReference type="CTD" id="100332644"/>
    <dbReference type="GeneTree" id="ENSGT00940000157165"/>
    <dbReference type="HOGENOM" id="CLU_045177_1_0_1"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003017">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00218">
        <property type="entry name" value="GlcAT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000010">
        <property type="entry name" value="Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF21">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 21 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 24" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="19">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="22">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="22">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="23">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="334" mass="38374" checksum="BF56A52D8F1F5EED" modified="2014-04-16" version="1">MPKRRDILAIVLIVLPWTLLITVWHQNAIAPLLAIRKDDGSDGRRDLGQGSDSKEYCSSDKDIVEVVRTEYVYTRPPPWSDVLPTIHVITPTYSRPVQKAELTRLANTFLHVPNLHWILVEDSQRRTPLVTRLLHETGLNYTHLNVETPRNYKLRGDMRDPRIPRGTMQRNLALRWLRETFNVNSTQPGIVYFADDDNTYSLELFEEMRSTRKVSVWPVAFVGGLRYESPKVNAAGKVYGWKTVFDPHRPFAIDMAGFAINLRLILFKPQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPNVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5R0</accession>
    <name>W5M5R0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01021468">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5R0"/>
    <dbReference type="FunCoup" id="W5M5R0">
        <property type="interactions" value="1009"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003718"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003725.1">
        <property type="protein sequence ID" value="ENSLOCP00000003718.1"/>
        <property type="gene ID" value="ENSLOCG00000003154.1"/>
    </dbReference>
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    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="13"/>
            <end position="161"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
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        <source>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="168" mass="18880" checksum="8F8226D8C16CA6A3" modified="2014-04-16" version="1">KIRKVCEMAVTLNTDLGDIKIELFCERSPRACENFLALCASGFYNGCIFHRNIKGFMVQTGDRTGTGKGGTSIWGRKFEDEFSEHLKHNVRGVVSMANNGPNTNASQFFITYAKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKTFRPLNDVRIKDVTMHANPFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M747</accession>
    <name>W5M747_LEPOC</name>
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        <submittedName>
            <fullName evidence="6">Gamma-crystallin M2-like</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000003557.1"/>
    </dbReference>
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        <property type="entry name" value="CRYSTALLIN, GAMMA M6-RELATED"/>
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    </dbReference>
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    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
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        <location>
            <begin position="41"/>
            <end position="83"/>
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    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="175" mass="21355" checksum="CA19633A620091AB" modified="2014-04-16" version="1">MGKIIFYEDRNFGGRSYECSSDCADMHSYFSRCNSIRVESGCWVLYERPNYMGYQYVLTRGEYPDYQRWMGYNDSIRSCRMFPDYQSSSYRMRIYEREGFGGQMMEFMDDCPNVYDRFHYRDIHSCNVMDGYWTFYEQPNYRGRQYFMRPGEYRRFSDWGANCATIGSFRRVMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7H8</accession>
    <name>W5M7H8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gamma-crystallin M2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7H8"/>
    <dbReference type="FunCoup" id="W5M7H8">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004336"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004344.1">
        <property type="protein sequence ID" value="ENSLOCP00000004336.1"/>
        <property type="gene ID" value="ENSLOCG00000003649.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXJY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163322"/>
    <dbReference type="HOGENOM" id="CLU_081883_1_1_1"/>
    <dbReference type="InParanoid" id="W5M7H8"/>
    <dbReference type="OMA" id="NDCARSC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003649">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
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    </dbReference>
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        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF129">
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    </dbReference>
    <dbReference type="Pfam" id="PF00030">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="22144" checksum="FE765E4E3A2A3CEC" modified="2014-04-16" version="1">PHQSSTMGKIIFYEDRNFGGRSYECSSDCADLHSYFSRCNSIRVESGCWMVYERPNYMGYQYFLRRGEYPDYQRWMGYNDCARSCRMIPHYQGSYRMRIYEREGFGGQMMEFMDDCPNVYDRFRYNDIHSCNVMDGYWMFYEQPNYRGRQYFMRPGEYRRFSDWGGMSPRIGSFRRLMDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9K0</accession>
    <name>W5M9K0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="10">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01011403">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9K0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005059"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005067.1">
        <property type="protein sequence ID" value="ENSLOCP00000005059.1"/>
        <property type="gene ID" value="ENSLOCG00000004227.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012547"/>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_1"/>
    <dbReference type="InParanoid" id="W5M9K0"/>
    <dbReference type="OMA" id="HGTVQRH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004227">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="9"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="310"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="11">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="464" mass="52285" checksum="F3CE3035D1CD4B02" modified="2014-04-16" version="1">MSKKIQAGSVVEMQGDEMTRVIWELIKEKLIFPYVGMDLHSYDLGIENRDATEDRVTVEAAEAVRRYNVGIKCATITPDEKRVEEFNLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAHGDQYKATDFVVPGPGRVEMKYTPKDGGEPLMFVVHDFQGSGGVALGMYNTDNSIRDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYEKEYQAQFEAKAIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGRTVEAEAAHGTVTRHYRMHQQGKETSTNPIASIFAWTRGLAHRAELDRNAELRAFCQALEAVCVETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFLDKLAENLKTKMASQPKLILSALHLLTRPSREQPTERTLSSCLIQSHPINGRRQRGPHCDVMCRDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9Y9</accession>
    <name>W5M9Y9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Selenoprotein H</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033731">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639038.2">
        <property type="nucleotide sequence ID" value="XM_006638975.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9Y9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005198"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005206.1">
        <property type="protein sequence ID" value="ENSLOCP00000005198.1"/>
        <property type="gene ID" value="ENSLOCG00000004349.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694570"/>
    <dbReference type="KEGG" id="loc:102694570"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287000"/>
    <dbReference type="HOGENOM" id="CLU_078295_3_2_1"/>
    <dbReference type="InParanoid" id="W5M9Y9"/>
    <dbReference type="OMA" id="LKFVPHG"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004349">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF17">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="45"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="140" mass="16255" checksum="4A5FEE3964786DB7" modified="2014-04-16" version="1">MRNQGGSARKRKAEVPQRRVPPPPSPPRRSGRAGEKPVKKRRYRPGTRALMEIRQYQKSTELLLRKGPFARVVREVCQQMFVMDYKWQGIAIGALQEAAEAFLVRLFQDSYLCSLHAKRVTLFPRDIQLARRIRGPEDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9Z6</accession>
    <name>W5M9Z6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elastin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tropoelastin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the elastin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9Z6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005205"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005213.1">
        <property type="protein sequence ID" value="ENSLOCP00000005205.1"/>
        <property type="gene ID" value="ENSLOCG00000004358.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYNR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168646"/>
    <dbReference type="HOGENOM" id="CLU_572310_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9Z6"/>
    <dbReference type="OMA" id="QPSRVYR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004358">
        <property type="expression patterns" value="Expressed in larva and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003979">
        <property type="entry name" value="Tropoelastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24018">
        <property type="entry name" value="ELASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24018:SF5">
        <property type="entry name" value="ELASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01500">
        <property type="entry name" value="TROPOELASTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867516" description="Elastin" evidence="11">
        <location>
            <begin position="26"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="44254" checksum="25C4956FCC7CF92D" modified="2014-04-16" version="1">MASRKIVSLLQGFLLFALHQPSLQGGVYLPAGVGGLYPGAGGVPGYKPGKPAAPFGAAGLGGTGLGPAGVGTGVGTGAVGKPPKPGGGLGAGGLGVGGLGAGGLGAGYKPAGYGGYYPGVGGVGTGLKPPKTVQPGTGVQPGVAVPGATGPGVGGPGTGLPQNGVSTGAVIPQIGLPGVGAGGKAGKVPGVGVPGIYYGGTFPGQGYAGRGILPGVPTGTGVKPKLPGGGYGNGGGPRQPGVFYGYPITAPKVPVYSYYQPWYGYRAYGYNQQVTYGGIAGKLPYGYGAGLGTGLPGGLGAGIKPGYPTGTGVGSQVISPAQAKAAKYGGVLPGTGVLPGAGFPVRGLSPGEYAAAKAAKYGVPGQVAVPGAVGTGGVPTAVGTGGVPGAVGTGVPVVPGKPAKEPTLAPGGTEIPVGTGVVQPGVGVIQPGVGTGVKPGVGTGVVQPGVGTGVVQPGVGTGVVQPGVGTGVKPGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAF6</accession>
    <name>W5MAF6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Centromere/kinetochore protein zw10 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="21">Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25 and ZWINT. Interacts with ZFYVE1. Interacts with RAB18 and this interaction is enhanced in the presence of ZFYVE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZW10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193095.1">
        <property type="nucleotide sequence ID" value="XM_015337609.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAF6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005365"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005373.1">
        <property type="protein sequence ID" value="ENSLOCP00000005365.1"/>
        <property type="gene ID" value="ENSLOCG00000004478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695821"/>
    <dbReference type="KEGG" id="loc:102695821"/>
    <dbReference type="CTD" id="9183"/>
    <dbReference type="eggNOG" id="KOG2163">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016427"/>
    <dbReference type="HOGENOM" id="CLU_012948_0_0_1"/>
    <dbReference type="InParanoid" id="W5MAF6"/>
    <dbReference type="OMA" id="HHLLTMG"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004478">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.150:FF:000001">
        <property type="entry name" value="centromere/kinetochore protein zw10 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048343">
        <property type="entry name" value="ZW10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048344">
        <property type="entry name" value="Zw10_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009361">
        <property type="entry name" value="Zw10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20666">
        <property type="entry name" value="ZW10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20665">
        <property type="entry name" value="Zw10_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06248">
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            <end position="406"/>
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        <location>
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        </location>
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            <begin position="615"/>
            <end position="769"/>
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            <begin position="124"/>
            <end position="151"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004186"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="770" mass="87808" checksum="39D3836D4E15C934" modified="2014-04-16" version="1">MASFVTEVLASSGKLEKEDLCCKINKLSQKVEEIKSEVCDMISKKYDEFLPSMQSAEDLMRQVDAVSRDMDNLKSCIENETQKSLRDAIDDFTDQKLRMEKNAAGIRVLRQLQQFDTAIEDYHRALLEKKYTEAAKQLEKAQSNVQVLKAKKGCELQILKALSTELTIQKQNLIYHLGQEWQKLAVWKLPSAKELTSVEAFMETELRLCCVGLKEDCSTESQLANVLQALAILGELNNKVKFFSQVLLKYVLKPLITYPALNVGMTGQPGQGTDLTFYCMETTVEHLSPSEAYIKVQAVLKELYTHLLNVTVGDKKLSVMLGDLIWEEMSDCIIRECLLYSIPANSSQLEQYEEVIKQTEEFEKALKEMHFLKGDSTDLLKYARNVNVHFASKKCQDVIVTARNLMTSEIHNTVKIFPETKVSIPKLPNLSSGDKTGQDKAVKMLRNETINLENENRLSHRTLSLPVCRISESVQKLIELAYHTLSEASNSTKQCAIQLFYTVRNIFQLFYDVVPTYHKENLQKLPQLAAIHHNNCMFIAHHLLTLGHQFRYHLPQPLCDGAATFIDLVPGFRRLGTECFLAQMRAQKVEMLERLSTARNFSNLDDEENYSAASKAVRQVIHQLKRLGKVWQDVLPVNIYCKAMGTLLNTAISEIITKITMLEDISTEDGDHLYTLCRTVIEEGPLVFTPLPEENKNKKYQEEVPVYVHKWMTFKELMIVLQANLQEIVDRWADGKGPLAMEFSPNEVKSLIRALFQNTDRRAAALARIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB38</accession>
    <name>W5MB38_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">Canopy FGF signaling regulator 2</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the canopy family.</text>
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    <dbReference type="EMBL" id="AHAT01018704">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000004671.1"/>
    </dbReference>
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    </dbReference>
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            <begin position="1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="183" mass="20903" checksum="823FF69E38FE5ADB" modified="2014-04-16" version="1">VRKMRGGVSALCVWLLLQASLGARQGQDMRCGACRALVDEMEWAISQVDPKKMIQTGSFRINPDGSQSVTEVPFARSEAHLLELMETVCETMTEYGERMDPNTHRKIYERVVSRDGKKMDLSQTRLDSQVTASLKFTCEYIVEHHEDELIEFFAHQTDNVKDKLCSKRTDLCDHALQIPHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBI0</accession>
    <name>W5MBI0_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Chromosome 1 open reading frame 146</fullName>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SCRE"/>
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        <property type="entry name" value="HYPOTHETICAL PROTEIN LOC689986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31408:SF2">
        <property type="entry name" value="PROTEIN SPO16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15162">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="Ensembl" id="ENSLOCP00000005739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="183" mass="20378" checksum="9F8D099F4AAFE0B6" modified="2014-04-16" version="1">LPALGSGTNKWKELXIASASLQSHETALALSAQQHRVRYSDSVEEGTYIFPLSGIAFMIVDTQECADSPGVSRLFEKIGKFIQVHRNSFLLFSSSLHAQREQEILFRIQQSFLGSKLRIIPVHNVAEMVKSMLTIAKATSKPHVDSVRDRMALARAQIIERSPVWDVLSRLQQSSAVSGFKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC59</accession>
    <name>W5MC59_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nucleoside diphosphate-linked moiety X motif 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield AMP and ATP. Exhibits decapping activity towards FAD-capped RNAs and dpCoA-capped RNAs in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GMP + GTP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57553"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5'-end CoA-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + (R)-4'-phosphopantetheine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67592"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="Rhea" id="RHEA-COMP:17276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
            <dbReference type="ChEBI" id="CHEBI:172371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67593"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end FAD-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + FMN + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67588"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="Rhea" id="RHEA-COMP:17275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
            <dbReference type="ChEBI" id="CHEBI:172372"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67589"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01012421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC59"/>
    <dbReference type="FunCoup" id="W5MC59">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005968"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005976.1">
        <property type="protein sequence ID" value="ENSLOCP00000005968.1"/>
        <property type="gene ID" value="ENSLOCG00000004965.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002416"/>
    <dbReference type="HOGENOM" id="CLU_037162_14_5_1"/>
    <dbReference type="InParanoid" id="W5MC59"/>
    <dbReference type="OMA" id="WRDYEQA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004965">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
        <property type="term" value="P:AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03428">
        <property type="entry name" value="NUDIX_Ap4A_Nudt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01405">
        <property type="entry name" value="TETRPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="150" mass="17141" checksum="AD4835D3DDCD2A08" modified="2014-04-16" version="1">MALRAGGLIVFRRLAQAVPDTIQFLLLQTSYGEHHWTPPKGHVDPGEDDMTAALRETEEEAGLGPAQLRLVDGYRRELRYEVRGRAKTVVYWLAELRDPGAAVRLSREHQDYRWLGLEQACRLAGHTDMQDALRAAHHFLSTDVHKYRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCX0</accession>
    <name>W5MCX0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Erythrocyte membrane protein band 4.1 like 4B</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCX0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006229"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006237.1">
        <property type="protein sequence ID" value="ENSLOCP00000006229.1"/>
        <property type="gene ID" value="ENSLOCG00000005168.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689414"/>
    <dbReference type="KEGG" id="loc:102689414"/>
    <dbReference type="CTD" id="54566"/>
    <dbReference type="eggNOG" id="KOG3530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158331"/>
    <dbReference type="HOGENOM" id="CLU_003623_5_0_1"/>
    <dbReference type="InParanoid" id="W5MCX0"/>
    <dbReference type="OrthoDB" id="6235974at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005168">
        <property type="expression patterns" value="Expressed in intestine and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13186">
        <property type="entry name" value="FERM_C_NBL4_NBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000024">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF18">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="73"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="618"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="693"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="642"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="839" mass="93836" checksum="47551AE794FE1626" modified="2014-04-16" version="1">MMGFLRRTFGRRSLRRYQTPDERDGRSRVAGGGGGGGIAALQAHQQQFIHTPVVVSGGAVVHIPAAGNSKSVITCRVLLLDGTDVNVDLPKTAKGQDLFDQILYHLDLIESDYFGLQFMDPDQVSHWLDVTKLIKKQVKIGPPYTLHFRIKFYSSEPNNLHEEFTRYLFVLQLRQDILSGRLKCPYDVAVELAAFCLQSELGDCDPSEHSPELVSEFRFTPKQTEAMEVDIFQKWAECRGKSPAQAELSFLNKCKWLEMYGVDMHFVKGRDGCEYALGLTPTGILVFEGSNKIGLFFWPKITRMDFKKSKLTLVVVEDDDKGREQEHTFVFRLDSAKACKHLWKCAVEHHAFFRLRQPAHGKSGRNDFVRLGSRFRFSGRTEYQATHGGRLRRRSTFERRPSKRYPSRRQSMVKGLNPVKTVHLSAQTSPDPTLYPYKQNIPTSHEQWHPTHTPLPTHLQHPGHTGSMSYPLTAVSPLNNTELQQYSIEENGGTPYSGKVSGRCHHHHHHHHHHGYGMVTTDSKEALAAVQCGSKLSTGIPCQYEETAAHLKQVEGSKVSGPWPALHININNKAEEKHLSEKSLHSPSSPSVIPDHLKCNILKAQMDAAFRVGTQALKEEGSPDPWRNSAGQEHNGRCPVSPQETAPSSTATEKQSVRPARTKKLIRQYSFNHEDEDDLPAALAAISSQSSTGKTASESSFEVQVQPPICVKSPTEGSGKMPLEPGDLLMDFTEATPLIKTVPSDPADTFYDPFSPVPQFSFSQAELPVLDVRPQCCDHPLSPAKPLQGPHGHTGRSVMELGPGLRPLHRNTVLMGSSEALRRELDREKLLKRLLMTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCX1</accession>
    <name>W5MCX1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pancreatic progenitor cell differentiation and proliferation factor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPDPF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639676.1">
        <property type="nucleotide sequence ID" value="XM_006639613.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCX1"/>
    <dbReference type="FunCoup" id="W5MCX1">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000006238.1">
        <property type="protein sequence ID" value="ENSLOCP00000006230.1"/>
        <property type="gene ID" value="ENSLOCG00000005173.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685829"/>
    <dbReference type="KEGG" id="loc:102685829"/>
    <dbReference type="CTD" id="336303"/>
    <dbReference type="eggNOG" id="ENOG502S1KD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009113"/>
    <dbReference type="HOGENOM" id="CLU_157362_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCX1"/>
    <dbReference type="OMA" id="ERCDIWT"/>
    <dbReference type="OrthoDB" id="9411431at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005173">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026754">
        <property type="entry name" value="PPDPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14572">
        <property type="entry name" value="PANCREATIC PROGENITOR CELL DIFFERENTIATION AND PROLIFERATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15060">
        <property type="entry name" value="PPDFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02071">
        <property type="entry name" value="PPDPFACTOR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="12543" checksum="0BBF6A20FB52580F" modified="2014-04-16" version="1">MAAIPSSGSLIATHDYYRRRLGSTSSNSSCGSSEYAGEVIPHHPGLPKHQDPGHWWSSFFFGKHNQPTMTTLTESPQNSGTFSVTNEKVTCGLAQEVMKRQLSDASESGKPEAGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MD56</accession>
    <name>W5MD56_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:ch211-103f14.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MD56"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006323.1">
        <property type="protein sequence ID" value="ENSLOCP00000006315.1"/>
        <property type="gene ID" value="ENSLOCG00000005242.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SHSG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164443"/>
    <dbReference type="HOGENOM" id="CLU_045259_0_0_1"/>
    <dbReference type="InParanoid" id="W5MD56"/>
    <dbReference type="OMA" id="NFPLNMT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005242">
        <property type="expression patterns" value="Expressed in zone of skin and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF21">
        <property type="entry name" value="SI:CH211-103F14.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867628" evidence="4">
        <location>
            <begin position="23"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="5">
        <location>
            <begin position="43"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="45136" checksum="68DB73D0335D8AAA" modified="2014-04-16" version="1">CVKSLKIVVKFIFLSHIKTSLSLTASDCGANFRPPEYTDISVECGTSTMSLAILICPAMYAGYNESLLIVNSIYDDPRCKGRIDGTVTPPVLRFSFPINESTACGSIFKTTSAPGTGIFSDFSNIQTVNISGVVRSNDPTTGTVTYNQELVYLYSCSYPLEYLINNTRIDVSGSSIAVKDNNGSFISTLSLQLYQDRNFTTPLIIPSAGIDLRSDVFVQVKAVNLTSKFNVLLDRCYASISQFPTNSTFFNLFVSCSRDSLTTMIENGDNQYARFSFKAFRFIEQKNQTVSTYYLHCITRLCDRSSCAQFKQCSSTGKKKRSVSDFTTPAPDGLSEPTTITSVPIITKSESVIQSKEDAVAPTIDEVRSISVGLGIAVGFLALISLVMITVAFMYYRKTKQSPATSKMFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDP7</accession>
    <name>W5MDP7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Aquaporin-8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>H2O2(out) = H2O2(in)</text>
            <dbReference type="Rhea" id="RHEA:74375"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>formamide(out) = formamide(in)</text>
            <dbReference type="Rhea" id="RHEA:74387"/>
            <dbReference type="ChEBI" id="CHEBI:16397"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>methylamine(out) = methylamine(in)</text>
            <dbReference type="Rhea" id="RHEA:74391"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Smooth endoplasmic reticulum membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013515">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDP7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006506"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006514.1">
        <property type="protein sequence ID" value="ENSLOCP00000006506.1"/>
        <property type="gene ID" value="ENSLOCG00000005397.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682656"/>
    <dbReference type="KEGG" id="loc:102682656"/>
    <dbReference type="CTD" id="447923"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159304"/>
    <dbReference type="HOGENOM" id="CLU_020019_3_5_1"/>
    <dbReference type="InParanoid" id="W5MDP7"/>
    <dbReference type="OMA" id="PAMVANH"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005397">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
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        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0046691">
        <property type="term" value="C:intracellular canaliculus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:smooth endoplasmic reticulum membrane"/>
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        <property type="term" value="F:urea transmembrane transporter activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:hydrogen peroxide transmembrane transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Aquaporin 8"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Aquaporin_8"/>
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        <property type="entry name" value="Aquaporin_transptr"/>
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        <property type="entry name" value="MIP"/>
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        <property type="entry name" value="MIP_CS"/>
    </dbReference>
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        <property type="entry name" value="AQP8A PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02020">
        <property type="entry name" value="AQUAPORIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0558">Oxidation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 22 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="73"/>
            <end position="91"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036281"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047305"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00074374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="263" mass="27639" checksum="6BF3266975E62176" modified="2014-04-16" version="1">MSRVESKSELFNMSAVSITEPEKEGERFCQKPNRFERYVQPCLAELLGSALFIFVGCSSVIENVEGTGRLQPALAHGLALGIVIAVLGEISGGHFNPAVSLSVYLTGGVSIILLLPYVGCQLCGGLIGAALAKAISTSQSYANASGAAFNVVQSDEQIGPAVVAETVMTLFLTMVVCMGAVNEKSRSLLAPLCIGLTVAADILAGGAVSGACMNPARAFGPAVVANYWDYHWIYWVGPVIGALLTACFVRLLLGDRKIRVLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDW5</accession>
    <name>W5MDW5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cdk5 and Abl enzyme substrate 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5MDW5">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006574"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006582.1">
        <property type="protein sequence ID" value="ENSLOCP00000006574.1"/>
        <property type="gene ID" value="ENSLOCG00000005450.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698857"/>
    <dbReference type="KEGG" id="loc:102698857"/>
    <dbReference type="CTD" id="564291"/>
    <dbReference type="eggNOG" id="KOG4164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022086"/>
    <dbReference type="HOGENOM" id="CLU_021942_2_1_1"/>
    <dbReference type="InParanoid" id="W5MDW5"/>
    <dbReference type="OMA" id="CRTKHIS"/>
    <dbReference type="OrthoDB" id="5353095at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005450">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20603">
        <property type="entry name" value="CYCLIN_CABLES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000020">
        <property type="entry name" value="CDK5 and ABL1 enzyme substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF3">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025798">
        <property type="entry name" value="Cables"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866099" evidence="6">
        <location>
            <begin position="17"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin N-terminal" evidence="7">
        <location>
            <begin position="417"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="60"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="528" mass="57565" checksum="8C209E9538C1171A" modified="2014-04-16" version="1">MLLVYLFPFIWRMAAAATCSSRSTLCHGSKAPKDHPRKSKDSRRRQAALSFLNNISLDGRPVDHLSNGNHSQRETELQAQEAGAAAAGHQVPASSCGASPQLPATPGGPAATQASRLAGVAVPPILVLPTEPGANTVVGSDAFSEGGNASNPLPAPGPLLLSPTGPPHAALPGGSSKPATLLSVQSCNSLSLDSRQRSRTLSGSPRPRGLKKVHFIKNMRQYDTRSSRIVFICAKRSLCAAFSVLPYGESFHISDPKLEGQRQRHPSGGGPPSLDMVPGLEGVELGADGKTVSYAKFLYPTNALVKQKNPPADPAAQTPLARSNIQRNYTPARISAAAPAQELGNEIVDMSEYDPNLLSDPQWPCGKHKRVLIFASYMTTVIEYVKPSDLKKDMNEMFKEKFPHIKLTLSKIRSLKREMRNVGEECSLQPVTVAMAYVYFEKLVLQGRLNKQNRKLCSAACVLLAAKIGSDLKKQEVKLLIDKLEERFRINRRELISFEFTVLVALEMALYLPESKVMPHFRRLIQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFC2</accession>
    <name>W5MFC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005141">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFC2"/>
    <dbReference type="FunCoup" id="W5MFC2">
        <property type="interactions" value="1848"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007081"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007089.1">
        <property type="protein sequence ID" value="ENSLOCP00000007081.1"/>
        <property type="gene ID" value="ENSLOCG00000005846.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074853"/>
    <dbReference type="HOGENOM" id="CLU_016218_0_2_1"/>
    <dbReference type="InParanoid" id="W5MFC2"/>
    <dbReference type="OMA" id="GDWESCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005846">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000001">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="307" mass="34180" checksum="DC48BF9286B84726" modified="2014-04-16" version="1">MSKTFLSPSELVEYFRNQMRQDPDMASAVAAIRTLLEFLKRDKGETILGLRENLTQAIDKLTSVDSSVAVSSGGELFLRFISLTSLEHPDLSQCKKVMIERGELFLEKISLSRNKVAKLCHTFIKDGAKILTHSSSRVVLRVLEKAAAEKKRFTVYVTESQPDTAGQNMAEKLRKLNVPVTVILDAAVGYVMEKVDLVIVGAEGVVESGGIINKIGTYQMAVCSKAHNKPFYVVAESFKFVRLYPLNQQDVPDRFKYKADTLKLSTNLAEEHPMIDYTPPSLITLLFTDLGVLTPSAVSDELIKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFD3</accession>
    <name>W5MFD3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic spindle assembly checkpoint protein MAD2B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitotic arrest deficient 2-like protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642034.1">
        <property type="nucleotide sequence ID" value="XM_006641971.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFD3"/>
    <dbReference type="FunCoup" id="W5MFD3">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007092"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007100.1">
        <property type="protein sequence ID" value="ENSLOCP00000007092.1"/>
        <property type="gene ID" value="ENSLOCG00000005872.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687800"/>
    <dbReference type="KEGG" id="loc:102687800"/>
    <dbReference type="CTD" id="10459"/>
    <dbReference type="eggNOG" id="KOG3186">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153395"/>
    <dbReference type="HOGENOM" id="CLU_050394_2_0_1"/>
    <dbReference type="InParanoid" id="W5MFD3"/>
    <dbReference type="OMA" id="CEDFPWI"/>
    <dbReference type="OrthoDB" id="21254at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005872">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000003">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="6">
        <location>
            <begin position="13"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="211" mass="24373" checksum="73B6D04E2DC6B1AD" modified="2014-04-16" version="1">MTTLTRQDLNFGQVVADILCEFLEVAIHLILYVREVYPTGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCIKPLIEKNDAEKVVVVIMDKEHHPVERFVFEISQPPLLSISSDTLLSHVEQLLRAVILKISVCDAVLDNNPPGCTFTVLVHTREAAIRNMEKIQVIKDFPWIVADEQEVHMREPRLIPLKTMTSDIVKMQLYVEERAQKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH91</accession>
    <name>W5MH91_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010752">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH91"/>
    <dbReference type="FunCoup" id="W5MH91">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007750"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007759.1">
        <property type="protein sequence ID" value="ENSLOCP00000007750.1"/>
        <property type="gene ID" value="ENSLOCG00000006414.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165296"/>
    <dbReference type="HOGENOM" id="CLU_014184_6_0_1"/>
    <dbReference type="InParanoid" id="W5MH91"/>
    <dbReference type="OMA" id="SENEMYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006414">
        <property type="expression patterns" value="Expressed in ovary and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    <sequence length="377" mass="43772" checksum="54F4D2B764267141" modified="2014-04-16" version="1">TQFRRSRPARAMGRCRVCACCLSEEERQAIAVDREIQKILQKQRKRDRREIKVLLLGTGESGKSTFIKQMRIIHGQGYSEEERRAFAKLVYQNIFTAAQSMISAMATLGIPYGRPENEAHAQRLLQVDVLQVRALELWEVQAIRSLWADLGIRQCYSQRRHFHLLDSTDYFMSNLERITAADYIPTSQDVLRVRLPTTGITEYPFTVEKMLLRIVDVGGQKSERRKWIHCFENVTSLIFLASLSEYDQVLEEQETDNRMEESKALFYTTIHSPWFLKSSIILFLNKKDILAEKILTSHLVTYFPQFKGKQCDVQAAMQFILQMYLQQAVKKDKRKGDRDKSIYSHFTCATDTNNIRSVFSAVKDTVLVKSLEEFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIR6</accession>
    <name>W5MIR6_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG14"/>
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        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
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        <property type="term" value="P:ephrin receptor signaling pathway"/>
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        <property type="entry name" value="EphR_LBD_A"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Galactose-bd-like_sf"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
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        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
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        <property type="entry name" value="Ephrin_lbd"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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        <property type="entry name" value="SAM_2"/>
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        <property type="entry name" value="TyrPK_ephrin_receptor"/>
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        <property type="entry name" value="FNTYPEIII"/>
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        <property type="entry name" value="TYRKINASE"/>
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        <property type="entry name" value="EPH_lbd"/>
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        <property type="entry name" value="Ephrin_rec_like"/>
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        <property type="entry name" value="TyrKc"/>
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        <property type="entry name" value="Fibronectin type III"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
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    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
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        <property type="entry name" value="EPH_LBD"/>
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    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
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        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
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    </dbReference>
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        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
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        <property type="entry name" value="SAM_DOMAIN"/>
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    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="542"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="21">
        <location>
            <begin position="31"/>
            <end position="209"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="327"/>
            <end position="433"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="436"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="622"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="19">
        <location>
            <begin position="906"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="747"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="628"/>
            <end position="636"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="654"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50011"/>
        </source>
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            <dbReference type="PROSITE" id="PS50105"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="976" mass="109722" checksum="F2487416142A637B" modified="2025-10-08" version="2">MLLIFCYKNLIFFFHKLSQKKKHLDIHFINMVTLLDSMSALGDLGWEAYPAEGWEEISVMDERNTPMRTYQVCNVMEANQNNWLRTSHIPRDGAQRVYIEIKFTLRDCNSLPGVPGTCKETFNMYYYESNNSNLWFIKESQYIKIDTIAADESFTQVDVGDRVMKLNTEVRDISNLSKKGFYLAFQDVGACIALVSVRVYYKKCPVTVLNLAEFPDTVTGGDSALVEVRGTCVNNSQEFDTPKMYCSADGGWLVPIGRCVCNPGFEERNDICQPCRPGFYKASAQDAYCMKCPLHSYSHQDKATECVCERGYYRAETDPRAMACTRPPSAPENPITTINETSVTLEWSPPRESGGRGDVTYSLHCRKCAADSRECGPCGSGLHFVPRQFGLAYTKAQVTDLLPHTSYTFDIEALNGVSDLSPSPKQYISVNVTTSQTVNVILKERKTKDSITLAWQGPERANGAIVEYEVIYYEKNQRDQNYTVLKTKSNSMTVDGLKPGTTYVFRVRARTDGGYGNYGGEIELETSHEDMLAIGDPNQPTILAVSIAGGIVLLIFLVACFVVSGRHCGYIKAKQDPDEEKMQFQHGRVKLPGSRTYIHPHTYEDPNQAVRDFAKEIDASAIRIERVIGAGEFGEVCSGRLKLPSKREIYVAIKSLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKCKPVMIITEFMENGSLDTFLKKHDGQFTIIQLVGMLRGIASGMKYLSDMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAIDEGYRLPAPMDCPVVLHQLMLDCWEKSRSDRPKFGQIVNTLDKLIRNPSSLKQLANSSVWQDPTTPEFSVNTVEDWLDAMKMTQYKENFSNAGYVTLDSVLYISVSELGKIGISLAGHQKKILSSIQSLQTQGAHVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK09</accession>
    <name>W5MK09_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Regulator of G protein signaling 4</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634998.1">
        <property type="nucleotide sequence ID" value="XM_006634935.3"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000008718"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008728.1">
        <property type="protein sequence ID" value="ENSLOCP00000008718.1"/>
        <property type="gene ID" value="ENSLOCG00000007206.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697169"/>
    <dbReference type="KEGG" id="loc:102697169"/>
    <dbReference type="CTD" id="5999"/>
    <dbReference type="eggNOG" id="KOG3589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159036"/>
    <dbReference type="HOGENOM" id="CLU_059863_3_0_1"/>
    <dbReference type="InParanoid" id="W5MK09"/>
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        <property type="component" value="Linkage group LG10"/>
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        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 4"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="63"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="209" mass="24036" checksum="9D6932DC242E8B99" modified="2014-04-16" version="1">MCKGLAALPATCLKSAKDMKHRFGFLLQKPDLMPDQNNANHKKEKSFGPKKICPGDAKKWGESLESLVNNEDGLAAFTTFLKSEFSDENIEFWMACEEYKKTTSQIKLASKAKKIYDLYIAVESEKEVNLDSSTREETRKNIEDPNITCFDEAQKKIFLLMEKDSYRRFLKSKYYQDLIQQTRTITSCGMEKKGKNHIPDFSLFLPQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKI7</accession>
    <name>W5MKI7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA primase</fullName>
            <ecNumber evidence="13">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.102"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01032423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKI7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008896"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008907.1">
        <property type="protein sequence ID" value="ENSLOCP00000008896.1"/>
        <property type="gene ID" value="ENSLOCG00000007341.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102692601"/>
    <dbReference type="eggNOG" id="KOG2851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011466"/>
    <dbReference type="HOGENOM" id="CLU_028288_3_2_1"/>
    <dbReference type="InParanoid" id="W5MKI7"/>
    <dbReference type="OMA" id="NVTRGFN"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007341">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000001">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="426" mass="49360" checksum="1F52DE19B7F0E270" modified="2014-04-16" version="1">MLKLASASEYDPASLPDLLPLYYRRLFPFSQYYRWLSYGGVVKSCFQNREFSFTLKDDIYVRYQSFSSQGELEKEIQKINPYKIDIGAVFSHRPNQHNTVKSGTFQALEKELVFDIDMTDYDDVRRCCSAADICSKCWTLMTIAIQILDRALREDFGFKHCLWVYSGRRGVHCWVCDEAARKLSATARSAVAEYLSLVKGGEDTIKKVQLPDLIHPFVMESLNVISRYFVDYAVVKQDILGSKESVDKVLALVPEDVKKELKTDFHNEKNPVKRWDRLVSHVERPQGKKGQHAAKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDPREVDQFDPFAVPTISLICQELDRPRAGEEEEEEAKDKENESAPTEKRRVRDYKKTSLAKYVRVFEQFVEKMERARRGELLKKSDLQRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLV5</accession>
    <name>W5MLV5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquinol-cytochrome c reductase complex assembly factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639828.2">
        <property type="nucleotide sequence ID" value="XM_006639765.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLV5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009364"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009375.1">
        <property type="protein sequence ID" value="ENSLOCP00000009364.1"/>
        <property type="gene ID" value="ENSLOCG00000007712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682666"/>
    <dbReference type="KEGG" id="loc:102682666"/>
    <dbReference type="CTD" id="55245"/>
    <dbReference type="eggNOG" id="KOG2873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018118"/>
    <dbReference type="HOGENOM" id="CLU_051390_3_0_1"/>
    <dbReference type="InParanoid" id="W5MLV5"/>
    <dbReference type="OMA" id="TWFLITE"/>
    <dbReference type="OrthoDB" id="4007at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007712">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="2">
        <location>
            <begin position="121"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="285" mass="32927" checksum="21B2FF812F83171D" modified="2014-04-16" version="1">MYRRVMQSAVRSGVSFTSHCGTLVKTRQMGACLSRTLEVHRRMELCSWPLSQSRTFHSTAQLFSVKQTPQASEEEVGAFTKLIEAMGFTGPLKYNKWKIKIAALRMYTCCVERINYDDFFEKCSLPDTLNSWFLVAQLHVWMCLVRMRQEGRAGKYMCRYIVHSMWEDVEQRGKIMGIDSVALKASMRAMTQNFYAAIFGYDEGVLSDDRVLAAALWRNLFNRQCEDPRQLELMVEYVRKQMQYIDSLDGEDLLLTGEVKWRPLVEENAQSILKPTSPTYNDAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM21</accession>
    <name>W5MM21_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Folate receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folate receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003023">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196708.1">
        <property type="nucleotide sequence ID" value="XM_015341222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM21"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009430"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009441.1">
        <property type="protein sequence ID" value="ENSLOCP00000009430.1"/>
        <property type="gene ID" value="ENSLOCG00000007764.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690865"/>
    <dbReference type="KEGG" id="loc:102690865"/>
    <dbReference type="CTD" id="100073336"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183144"/>
    <dbReference type="HOGENOM" id="CLU_070826_1_0_1"/>
    <dbReference type="InParanoid" id="W5MM21"/>
    <dbReference type="OMA" id="KDDFTCK"/>
    <dbReference type="OrthoDB" id="567542at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007764">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004269">
        <property type="entry name" value="Folate_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517">
        <property type="entry name" value="FOLATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517:SF14">
        <property type="entry name" value="FOLATE RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866466" evidence="5">
        <location>
            <begin position="23"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="251" mass="28873" checksum="59E8D8360EC698E0" modified="2014-04-16" version="1">MYLRREMLGVTLLLALVSVAVSEDILNKCMDGKHHKKTPGAEGKLFQQCKPWKSNACCTANTTEEAHEDNSYLYNFNWNHCGIMSSSCKRHFIQDTCFYECSPHLGPWIQKVNQSWRNERIIDVPICKEDCNGWWEDCKNDLTCKENWHKGWDWSTGTNICPAGTKCRKFTDVFPTPQSMCEKIWSRSYAYSMFERSSGRCMQMWFDEGTNPNEDVARYYAVEKGLINGAASFSLPASLFGLLAFLSLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM51</accession>
    <name>W5MM51_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01020087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636582.1">
        <property type="nucleotide sequence ID" value="XM_006636519.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214412.1">
        <property type="nucleotide sequence ID" value="XM_015358926.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214413.1">
        <property type="nucleotide sequence ID" value="XM_015358927.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214415.1">
        <property type="nucleotide sequence ID" value="XM_015358929.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM51"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009460"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009471.1">
        <property type="protein sequence ID" value="ENSLOCP00000009460.1"/>
        <property type="gene ID" value="ENSLOCG00000007779.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691430"/>
    <dbReference type="KEGG" id="loc:102691430"/>
    <dbReference type="CTD" id="557765"/>
    <dbReference type="eggNOG" id="KOG1151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182984"/>
    <dbReference type="HOGENOM" id="CLU_000288_85_1_1"/>
    <dbReference type="InParanoid" id="W5MM51"/>
    <dbReference type="OMA" id="YKHACRE"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007779">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14040">
        <property type="entry name" value="STKc_TLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000037">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOUSLED-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="466"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="355"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="775" mass="87563" checksum="7866F23EB0698F28" modified="2014-04-16" version="1">MSVQSNSGSGGSLEGTPSWSRLSTSPTPGLLSQQHITAPVKTKEGAMDELHSLDPRRQELLEARFTGGVSGSTGGSTGSGSAGAKALTNNECSNHSFGSIGSLSDKESEGSDVKRGSSPAYSTPEKKLSESSRGRKRKAENQTESNQGKGVRGPKISDYFDLQGGNGSSPVRGVPPAIRSPQNSHSHSTPGSNIRQNSPSPTSLTFVDHMVVHPKQLSIKCVQTDLTVLKLAALESNKNLDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCITMSKKLLIEKSTQEKQACREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWINQQREEIERQRKLLAKRKPPTTNNSQTPTTNSEPKQRKTKAVNGAENDPFLKPSLPQLLTVAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLFEQRYAAVKIHQLNKNWREEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDNYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLASDSYLLPHMRRSNSSGNLHMGGSGPPVPPSSSIISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM83</accession>
    <name>W5MM83_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Trafficking protein particle complex subunit 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM83"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009492"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009503.1">
        <property type="protein sequence ID" value="ENSLOCP00000009492.1"/>
        <property type="gene ID" value="ENSLOCG00000007814.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006486"/>
    <dbReference type="HOGENOM" id="CLU_004738_0_0_1"/>
    <dbReference type="InParanoid" id="W5MM83"/>
    <dbReference type="OMA" id="HISLPQH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007814">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="5">
        <location>
            <begin position="186"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="6">
        <location>
            <begin position="363"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="7">
        <location>
            <begin position="544"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="834"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="855" mass="94852" checksum="A258BAD4753211CD" modified="2014-04-16" version="1">MSVPDYMQCAEDHQTVLVAVQSVGLVPEDQFFRIYKRIASVSQVSIRDSQRVLFIRYRHHYPPENNDWGDFQTHRKVVGLIAVTECSAAKDWPQTSERFHGQKEVFGSTLYDSRLLVFGLRGEVTEQARTDVAFYPSYGECEDVERRVEDFIESIFIVLESKRLDRATDKSGDKIPLLCVPFEKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGMLQDALVHYHMAVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTAGKAGGRKPSLSQPSDAGKRHRPGAQEVLIDPGPGALTTNGISADTSSEIGRAKNCLSPEDIIDKYKEAISYYGKYKNAGVIELEACVKAVRVLAIQKRAMEASEFLQNAVYINLGQLSEEEKIQRYSILSELYGLIGFHRKSAFFKRVAAMQCVAPTIPEPGWKACYKLLLETLPGYSLSLDPQDFSKGSHRGWAAVQMRLLHELVYASRRMGNPGLSVRHLSFLLQTMLDFLSDQEKKEVTQSLENYTSKCPGGMEVITLPDGLKLPPVPFTKLPIVSNRSVKLLNLPISLRPHKVKSLLGQNMATKSPFIYSPIIVHNRGEEKGKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPRTAGNITVNGYHTSVFGVTSDCLLEHLAGVKTSGCTVEVVPALPRLQLGTSLPRSAHTLQLSSGEEVSTSVSVQLFNGETQQLIIKLENIGTELLESLEITSKTVNTKEKLFGDFLSWNLEDTLSQFPLKPGQVATLTVNIRVKLDFSGQENLLQDLNDDGISVTGLPISSPLRQVLKPRAESKTVNPSEASKAGEYNHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMG5</accession>
    <name>W5MMG5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bardet-Biedl syndrome 2 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="840" mass="96451" checksum="EB23D2068527BB85" modified="2014-04-16" version="1">AMLRLRSVCRSSWRLLHPRVAGSDQVLEQLFASGSEEQVLDVVGRNKAKLSAAHVGLAVSRLWQFQKEKPQMLRTLEFTRTHPQFLALCVLAENKVEQLPDSELVDMLYNVLRLSVEPHDSLVQELVTEGWRRIDRLPMAPLSKFANCLNDQHLNSSPLMGEITEIVSQKLDSVKDARVLSILMIGVSALVSPRLRDALIQKMDFLLDTTGPIHFNTPRRVIQFLRNVKHSYRPLMEKCNRILLRSAKDLDAENISIILGLYQSLQFNNCDFRLAAKRRLSELLDSCTDPVSFTRLFAALGPMAGQETRERLESSALLLAEELNSQQALALAETMEEMECRNLQLINKIGTIFSKNLEGYKPVEIARITQVLILLHYQNPELLTKLRCFLVSHLQVSVFPYEVTMLTRVLSMLPSPRLDEEVTSRVDDLLPQCNLSDLNTFATAMAKWVRHDPSYRHVMPRVYVKLLQKLNHCGLERLRKADSLDLLLEELKYVPGEWFEEVLLEETMLSLQRLIDQISWSNVPELAVFLTRTNHLCTPLLDRIASVSMEHIEKIHYSAMYATILPFTVLNYDPPQGDEFFDACIQHLRPHISSFDPHLLVLLAYSLAVADCFPEDVIREIFNVDFLAKLDAQLETLPDALNMRIRLRLMELNRAVSLECPEFQIPWFHERYCQQLLKKGNGSVTAVQQQIHKMLGEILGGMSYAKVSVLTPYFYVVEFECILDRNKKPIPYVEHSRLLITEEGKVQWGPGSPVNDRSELPPGAQRVAVDFLDSKSFCKNSRHMKGEAVMRKRHLEILGYNVAQIPHFEWNSMELSTRDAWKEYLRKKIFSSGVKRREIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNE5</accession>
    <name>W5MNE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EMI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2024-03" db="PDB data bank">
            <title>Structure of a triple-helix region of human collagen type VII.</title>
            <authorList>
                <person name="Zhu Y."/>
                <person name="Yang X."/>
                <person name="Sun F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 150-176</scope>
        <scope>HYDROXYLATION AT GLN-151; PRO-154; PRO-169; PRO-172 AND PRO-175</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="8YUK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.78 A"/>
        <property type="chains" value="A/B/C=150-176"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNE5"/>
    <dbReference type="SMR" id="W5MNE5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009904"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009916.1">
        <property type="protein sequence ID" value="ENSLOCP00000009904.1"/>
        <property type="gene ID" value="ENSLOCG00000008161.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSR5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158302"/>
    <dbReference type="HOGENOM" id="CLU_045268_1_0_1"/>
    <dbReference type="InParanoid" id="W5MNE5"/>
    <dbReference type="OMA" id="FVEPRWS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008161">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="4">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="4">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="4">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="4">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="8YUK"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="37124" checksum="8BA50940291A3898" modified="2014-04-16" version="1">PGNNLEAQEAIRKQAALRQPLARSRDPPSGCLNCSRIATLTNRLNSLEAKVLVLTNPSTPPHSHLQAKGAPAAESSGLWGASPAKGIPGEQGPTGPRGERGEDGTPGRNGLPGSRGLPGPSGPRGEAGVRGPAGAPGSRGIPGPPGIQGPQGPPGLPGERGFRGSPGPPGPPGPPGPPGPPAHARISDPGYLGDHSDTSDSLLSNSFLDSRGTPARGPEGSPGPPGPAGPPGPQGPAGPPGPPGLPGQNGQPGTPGLQGPAGPKGDKGDRGPLGYPGERGFRGEPGSSADQSLPVLKGAGDGIHQIREALKILAERVLILETMIGIHEPDVGSGEGQYGTPLPGFYRDKRSKISAYQALSSTLSREAEEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNZ3</accession>
    <name>W5MNZ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Gamma-aminobutyric acid type A receptor subunit gamma3</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003396">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003398">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003399">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNZ3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010102"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010114.1">
        <property type="protein sequence ID" value="ENSLOCP00000010102.1"/>
        <property type="gene ID" value="ENSLOCG00000008316.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079478"/>
    <dbReference type="KEGG" id="loc:107079478"/>
    <dbReference type="eggNOG" id="KOG3642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155607"/>
    <dbReference type="HOGENOM" id="CLU_010920_2_0_1"/>
    <dbReference type="InParanoid" id="W5MNZ3"/>
    <dbReference type="OMA" id="HERINLH"/>
    <dbReference type="OrthoDB" id="203862at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008316">
        <property type="expression patterns" value="Expressed in brain and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022851">
        <property type="term" value="F:GABA-gated chloride ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904862">
        <property type="term" value="P:inhibitory synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051932">
        <property type="term" value="P:synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19000">
        <property type="entry name" value="LGIC_ECD_GABAAR_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19054">
        <property type="entry name" value="LGIC_TM_GABAAR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000003">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005440">
        <property type="entry name" value="GABBAg3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005437">
        <property type="entry name" value="GABRG-1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01620">
        <property type="entry name" value="GABAARGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01623">
        <property type="entry name" value="GABAARGAMMA3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="75"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="287"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="510" mass="59314" checksum="911600B1196DB246" modified="2014-04-16" version="1">MYSCIFYPLLFRFSTAFTLTNSWNRLLINMPKLLVYFLLLSVFHACSQTIEYEEEYEDVTVNQMLAPKTQETDATQILNNLLKEYDKKLRPDIGIKPTVIDVDIYVNSIGPVSSINMEYQIDIFFAQTWTDSRLRFNSTMKILTLNSNMVGLIWIPDTIFRNSKTADAHWITTPNQLLRIWNDGKILYTLRLTINAECQLQLHNFPMDEHSCPLIFSSYGYPSDEMVYKWRKNSVEAADQKSWRLYQFDFMGLRNTTDVIKTSSGDYVVMTVYFDLSRRMGYFTIQTYIPCILTVVLSWVSFWIKKDATPARTALGITTVLTMTTLSTIARNSLPRVSYVTAMDLFVTVCFLFVFAALMEYATLNYYSSVRKPSCTKKKKSSLPTFFALSKEVFWQHTPPPMNCLIIHAKLQSIAYRQNYSVLDVGPPPTVITLNNSMYWQEFEEACVYECLDGKDCQSFFCCYEECKSGAWRKGRVHIDILELDAYSRVFFPTSFLLFNLVYWVGYLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPL9</accession>
    <name>W5MPL9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase DDX54</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DEAD box protein 54</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts in a hormone-dependent manner with nuclear receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01005287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPL9"/>
    <dbReference type="FunCoup" id="W5MPL9">
        <property type="interactions" value="1765"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010328"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010341.1">
        <property type="protein sequence ID" value="ENSLOCP00000010328.1"/>
        <property type="gene ID" value="ENSLOCG00000008485.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686496"/>
    <dbReference type="KEGG" id="loc:102686496"/>
    <dbReference type="CTD" id="79039"/>
    <dbReference type="eggNOG" id="KOG0337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075100"/>
    <dbReference type="HOGENOM" id="CLU_003041_5_2_1"/>
    <dbReference type="InParanoid" id="W5MPL9"/>
    <dbReference type="OMA" id="EDQFGMM"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008485">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="112"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="311"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="704"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="751"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="841"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="851" mass="94874" checksum="2FF880880DA48D65" modified="2014-04-16" version="1">MAQRKKRFTRKKRHAAHREPDADSDGGDFELAAEASCDDTATKKVGKKLPRFPAPSDCLSDVEPDTRELVRAQNKKKKKSGGFQSMGLSYPVFKGVMKKGYKVPTPIQRKTIPLILEGRDVVAMARTGSGKTAAFLIPLFERLKAPQAQTGARALILSPTRELALQTLKFTKELGKFTGLKTALILGGDRMDDQFAALHENPDIIIGTPGRLMHVAVEMNLKLRGVEYVVFDEADRLFEMGFAEQLQEIIRRLPEARQTLLFSATLPKLLVEFARAGLTEPVLIRLDVDTKLSDQLKLAFFQLRQDDKPALLLHLLRSVVKPQEQTVVFAATKHHVEYLKEVLSSEGVDCSYIYSALDQTARKINIGRFVHRKCMVMLVTDVAARGIDIPLLDNVINYNFPSKAKLFLHRVGRVARAGRTGTAYSLVCPDEVPFMFDLHLFLGRPLQFATAGLPQDVDGVFGRVPQSVLDDEEALLIAAHQNSQDLQNLRRVAENAYKQYLRSRPSPSAESIKRAKGVDLSSLAVHPLLGSALGGEELDRLKMVDGVKNYKSKATIFEINSSSRTAASEVMRAKRRRDAGQVGSFLRAREDKAAEGPASQPAAGAALQAESDEEHIQGVFSAVLGGKRKMTQGEAEAPKKKKKRETFCDQEFYIPYRPKDFDSERGLSLVGEGSTFEQQASGAVLDLMGDEGDKMSQHKNMLKWDRKKKRFVRETGKEDQKKKVRTESGQLISSSYKKNLYEQWKKKYKMEDGASDSEGEGGGSGARRASTGRKGGRGPQPQKHSPGPRPRSELKSRDQILKQRKKREKQQFLQSGGLKKLRRKNRQHVGEVARSGFGRGTSRKGKLRKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPZ9</accession>
    <name>W5MPZ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger protein 608</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01002765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000010473.1">
        <property type="protein sequence ID" value="ENSLOCP00000010458.1"/>
        <property type="gene ID" value="ENSLOCG00000008603.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSUK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008748"/>
    <dbReference type="HOGENOM" id="CLU_004142_1_0_1"/>
    <dbReference type="InParanoid" id="W5MPZ9"/>
    <dbReference type="OMA" id="PNCAMDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008603">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040010">
        <property type="entry name" value="ZN608/ZN609"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564">
        <property type="entry name" value="BRAKELESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1382"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="922"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="979"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1395"/>
            <end position="1411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1469" mass="159019" checksum="5B16C1B02EF4F8FE" modified="2014-04-16" version="1">MSVNSSTGGKSVDPNAVDTYDSGDDWEIGVGNLIIDLDADLEKDRQKLEMNNSTNNASSKDCGGLTSSGANATSALSDGLKFTSVQPPIPQGNSNKETGKSKVKRSKTSKDTNKSLSSAALYGIPEISGGKRQEVQGRPGEASGMNSALGQAVSNPNTSNSIASCAKGKEEKNGKNQSRGSKRDKDASRARKEKHDIGHANTNASCTTGHLYGFGAKGSPYHCGNVGVGDINKSGLDSGIMGNSIVVKKEENDEEETHRPAKKLKTEKVDPLFTVPAPPPPITTSIQPQILPSFFPPTSNISAPTEQLLVRTRSVGVNTCEVGVVTEPECLGPCEPGTSVNLEGIVWHETEEGVLVVNVTWRNKTYVGTLLDCTKHDWAPPRFCESPTSDLDMRGGRGRGKRARSVVANAPGIEPSFAEARGLQHKNRGGGANGKGRRGSLNSSSGRRTPPNCTLEEIKASPTANNKRKNKPSMELDLNSSSEDTKSGKRVRTNSRSTPTTPQGKSEGSFLDQGCSSPVLIDCPHPNCNKKYKHINGLRYHQAHAHLDPENKLEFEPESEDKISDCEESLGNVALECTDTTFGSLKGPNSPGNLGAAGTPKGKREMLSNGQSPVMNSKMGRSSGKKKGHIHDMNNLPIISNMTVALDNCVVSDGSSVSEMPKLEAEGIIDKKGVCEKDKGKKANNGKVDKSLSKPKTTRPIAPAPPPPQLIAIPTATFTATTTGTIPGLPSLTTTVVQATPKSPPLKPIQPKPTIMGEPSTVNPALASLKDKKKKEKRKLKDKECKEAGSPKMDSKLAKIEDSKSAGKEIPSHFLKEHLNKNEVLPNGLSESQESRMASIKAEADKVYTFTDNAPSPSIGSSSRLECGTLVNGQSPMTPLHVLMQNGSDGTGTKTNSPAYSDISDAADDGGSDSRSEGVRSKASSPSEINSNKESVVKSLPATPAQAAPGKESHSPYYHGYDPYYSPNYMHSGQPSTPASGNGSNSQGNKSKKEGSEEEGEKKEKAEALALDAKKNEMINSNLQAQHQSVITQRHPALAQSLYYGQYPYGLYMDQKSLMASNTYRQQYEKYYEDQRLAEQKMTQGNREGERKGDLPQKEQSKEDIKQKTIPSATVSKAPPTPDSNKNCNSKCGPGIPAKTEESGKQQILTNQQQHLQSAKQMENHQLIKEAVEMKSVMDSMKQTGVDPTLRFKQESESRSWHHYPYQAKYMEQQKTEELEKKAKDDCPNKAGGKDISGLNLTVSMTTVKDEPKDLKRQDSHLGSVEDSKKSDERGTPVSGKDSRNARVAVSSPMSQHQSYIQYQHAYPYLQMYDPNHPAYRAVSPVLMHSYPGKHPNESFHYPIYGKMAGRDEVEKTNTSPSISTKPASESKALDLLQQHANQYRTKSPGPAEKGPPERERETERERDRHSPFARHLHTHHHTHLGMGYPLIPGQYDPYPGLTSAALVASQQVAAQASASGIFPTQRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQY5</accession>
    <name>W5MQY5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metabolism of cobalamin associated A</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">GTPase, binds and hydrolyzes GTP. Involved in intracellular vitamin B12 metabolism, mediates the transport of cobalamin (Cbl) into mitochondria for the final steps of adenosylcobalamin (AdoCbl) synthesis. Functions as a G-protein chaperone that assists AdoCbl cofactor delivery from MMAB to the methylmalonyl-CoA mutase (MMUT). Plays a dual role as both a protectase and a reactivase for MMUT. Protects MMUT from progressive inactivation by oxidation by decreasing the rate of the formation of the oxidized inactive cofactor hydroxocobalamin (OH2Cbl). Additionally acts a reactivase by promoting the replacement of OH2Cbl by the active cofactor AdoCbl, restoring the activity of MMUT in the presence and hydrolysis of GTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Interacts with MMUT (the apoenzyme form); the interaction is GTP dependent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015423">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQY5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010794"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010809.1">
        <property type="protein sequence ID" value="ENSLOCP00000010794.1"/>
        <property type="gene ID" value="ENSLOCG00000008866.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685652"/>
    <dbReference type="KEGG" id="loc:102685652"/>
    <dbReference type="CTD" id="166785"/>
    <dbReference type="eggNOG" id="ENOG502QR2W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009908"/>
    <dbReference type="HOGENOM" id="CLU_043725_2_2_1"/>
    <dbReference type="InParanoid" id="W5MQY5"/>
    <dbReference type="OMA" id="WMWERID"/>
    <dbReference type="OrthoDB" id="1476984at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008866">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03114">
        <property type="entry name" value="MMAA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000647">
        <property type="entry name" value="Methylmalonic aciduria type A homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006958">
        <property type="entry name" value="PRK09435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23408:SF3">
        <property type="entry name" value="METHYLMALONIC ACIDURIA TYPE A PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23408">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="407" mass="44240" checksum="F6E4D25B3429C0F5" modified="2014-04-16" version="1">MGCSGLLSRLRHLGSLSQTRPPRRWCVSGAAHVTLWPGGGGSGAWVSAERPRWYCVEAALGQQAGELEARERRQLAQLYEGLIQGHRACLAQSITLVESRHPRKKELAQVLLQQVLAYRKRQEELNGGKPLAFRVGLSGPPGAGKSSFIEVVGKMLTGRGHKVSVLAVDPSSCTTGGSLMGDKTRMTELSRDMRAFIRPSPTSGTLGGVTRTTNEAIVLCEGAGYDIVLVETVGVGQSEFAVADMVDMFVLLIPPAGGDELQGIKRGIIEMADLVVVTKADGDLLVPARKIQAEYTSALKLLRKKSKTWQPKVVQVSSRTGQGVPELWAMLTEFQEAMLRSGELVSRRQGQQKVWMWNVIQESVLRLFQEHPAVREQIPELERRVTSGALSSGLAADMLLKAFSAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSG8</accession>
    <name>W5MSG8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">EBF transcription factor 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms either a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the COE family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027414">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSG8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011327"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011343.1">
        <property type="protein sequence ID" value="ENSLOCP00000011327.1"/>
        <property type="gene ID" value="ENSLOCG00000009282.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3836">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182859"/>
    <dbReference type="HOGENOM" id="CLU_016320_3_1_1"/>
    <dbReference type="InParanoid" id="W5MSG8"/>
    <dbReference type="OMA" id="FGSTTVW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009282">
        <property type="expression patterns" value="Expressed in testis and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11606">
        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001696">
        <property type="entry name" value="Transcription factor COE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032200">
        <property type="entry name" value="COE_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032201">
        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IPT/TIG" evidence="14">
        <location>
            <begin position="262"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="604" mass="65830" checksum="3741CC9EDCF6C1E8" modified="2014-04-16" version="1">MFGIQESIQRSGSSMKEEPIGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTSFLGFVEKEKESNGEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIIYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHAATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYNVPRNHNQLPGLGNTSVHAGMMGVNSFTGQLAVNVSESSQAANQGFSRNTSSVSPHGYVPSTTPQQTNYSSVTTSMNGYANTGMSNLGGSPSFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSANGNSLQDQSFVDSSKYSTAGSLQGLAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT96</accession>
    <name>W5MT96_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a heterodimer with smc6. Component of the SMC5-SMC6 complex which consists at least of smc5, smc6, nsmce2, nsmce1 and nsmce4a.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MT96"/>
    <dbReference type="FunCoup" id="W5MT96">
        <property type="interactions" value="1566"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011605"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011622.1">
        <property type="protein sequence ID" value="ENSLOCP00000011605.1"/>
        <property type="gene ID" value="ENSLOCG00000009494.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687536"/>
    <dbReference type="KEGG" id="loc:102687536"/>
    <dbReference type="CTD" id="23137"/>
    <dbReference type="eggNOG" id="KOG0979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074816"/>
    <dbReference type="HOGENOM" id="CLU_004969_1_0_1"/>
    <dbReference type="InParanoid" id="W5MT96"/>
    <dbReference type="OMA" id="RFWTSQP"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009494">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Structural maintenance of chromosomes 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000793">
        <property type="entry name" value="Structural maintenance of chromosomes protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="15">
        <location>
            <begin position="50"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="402"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="647"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="776"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="874"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1081" mass="126144" checksum="031D017E89015954" modified="2014-04-16" version="1">MAASNKRKRVSHQFANSNNSEGASTSRANNTLHSVGDDKKDHFVEGSIVRISMENFLTYDRCDVYPGPNLNMIVGANGTGKSSIVCAICLGLAGKTGFLGRGDKVGLYVKRGCSKGSIEIELYKCEGNLKINREIHVENNQSTWMINGKHASQKTVEEAVKALQIQVGNLCQFLPQEKVGEFAKMSKVELLEATEKSVGPPEMYRFHCELKNFRSKERELENICKEKAGLLEKMGHQNERYKRDVERYYEKQRHLDVINMLERKKPWVEYETVRRQILAVKSERDKVKSELKKLQDEQAPMLNKIKSVESQLKPLDNKMKEKNIQMKEAWQKCKIKQDQLDRKDKEIEDIQQALSLKQTEEADRQKRINNTRRMIDDWQAELNSIGSQDDVQPKIDSINGQLRHIQEEKAKIDSERADVKREWENQKYEREKLKIELRKHEDLMIRKEEKLKERYRDTYNAMMWLRENRNLFRGNVYEPIMLVVNVRDAQHAKYIENQVSINDLRAFVFQKQEDMEAFMSEVRDKQKLRVNAVIAPPQSCANKPPPRPIEALRRYGFFSYLRELFDAPEEVMSYLCNQYKVNEIPVGNEQTKAMIETVIKESQLRQIFTAEEKYTVKKSVYSNKTITSNTALRPSQFLTSAADADTRRQLEGQLQTAQQKLQELELQMRAFAEQQTQLDRRDNDLRNQKKELSELKGKKRQLEQKISTKLDSLKQMEQNGINLKEEEGKTKEKIKAVNTEKVTIVSDLIESMKKRVKLNMDRVYLALDSVQLTAKKTKLETDCREASARLRVLEDKYLELERKKHTLVCDCKGLMKKAQEICNLDPGQSDVPQDLHDVFKQFPDTVDEIDALLHEERSRAECFTGVNSSVLDEYAERTQEYDNLKVELDKKTKALQEYRESISQVKERWLNPLKLLVEQVNEKFSDFFRSMQCAGEVDLHSDNEEEYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMSLQELNRCPFRVVDEINQGMDPINERRVFDIVVRTACKETTSQYFFITPKLLQNLTYADEMTVLCVHNGPYMLPPAKWNLKAFERRRQRVQRLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTM2</accession>
    <name>W5MTM2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="12">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with IGSF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01003854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011731"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011749.1">
        <property type="protein sequence ID" value="ENSLOCP00000011731.1"/>
        <property type="gene ID" value="ENSLOCG00000009590.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156572"/>
    <dbReference type="HOGENOM" id="CLU_000869_0_0_1"/>
    <dbReference type="InParanoid" id="W5MTM2"/>
    <dbReference type="OMA" id="INHTLTM"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009590">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21062">
        <property type="entry name" value="BTHB_HectD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000051">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000018">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.80:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000267">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000007">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041200">
        <property type="entry name" value="FKBP3_BTHB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010606">
        <property type="entry name" value="Mib_Herc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037252">
        <property type="entry name" value="Mib_Herc2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18410">
        <property type="entry name" value="BTHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159034">
        <property type="entry name" value="Mib/herc2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51416">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="426"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="MIB/HERC2" evidence="15">
        <location>
            <begin position="1266"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="14">
        <location>
            <begin position="2102"/>
            <end position="2561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="487"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="627"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1287"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1386"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1443"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1507"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1627"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1728"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1309"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1325"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1388"/>
            <end position="1411"/>
        </location>
    </feature>
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        <location>
            <begin position="1454"/>
            <end position="1463"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1509"/>
            <end position="1535"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1628"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="2530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065700"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2561" mass="283794" checksum="12DF010DFA11BB28" modified="2014-04-16" version="1">MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLSFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLETCVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSTCKPGRTSTGATATAADSKVSNQVSTIVSLLSTLCRGSPLVTHDLLRSELPYSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDVNKEEEEGNEPKGDPEMAPIYLKRLLPVFAQTFQETMLPSIRKASLALIRKMIHYCSEALLKEVCDSDAGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDVFLDQLARLGVINKVSTLAGPTSDDENEEESKPEKEDEPQEDAKEIQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARSQVKPATASQPILSALGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVKTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESAWELHTNRQCIEGENMWRDLMKTALENLIVVLKDENTISPYEMCSSGLVQALFTVLNNSVDLEVKHDCRPLMERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQMFTFRHQHDFDENGIVYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDSSALNCHTNDDKNAWFALDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTTLYTHVDDCSLNEPGSTATWPLDPSKDEKQGWRHIRIKQMGKNASGQTHYLSLSGLELYGTVSGVCEDQLGKAVKEAEANLRRQRRLFRSQVMKYIVPGARVVRGIDWKWRDQDGNPPGEGTVTGEAHNGPAQSWSSLVKNNCPDKSATAGASSSSRKGSSSSVCSVASSSDISLSSAKLERRSESVLEHGTAPSAESHEPIVVLSAADSSPHAEGGSASSASTSTLTADTGGENADRKLGADAAVRPTGESGAISMGIVSISSPDVSSVSESSSKEATSQRPLCSAANTRLSVSSLLAAGAPMSSSASVPNLSSREASLMESFVRRAPNMSRTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTTGTTSTVTMSTSIVTSSNNVATATTGLSVGQSLSNTLTTSLTSTSSESDTGQEAEFSLYDFLDSCRANTLLAELDDEEDLPEPDEDDDENEDDNQEDQEYEEVLVRSRVNLGFHILMNSVKHNLVAAMTIKVLLQHCVSKEEEEYETKGGRRRTWDDDFVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPRSEILEEVECAPSPHLALILKVAGLGTTREVELPLSNYKSTIFYYVQKLLQLSCNGSVKSDKLRRIWEPTYTIMYREMKDSDKGKESGKMGCWSVEHVEQYLGTDELPKNDLITYLQKNADSTFLRRWKLTGTNKSIRKNRNCSQLIAAYKDFSEHGSKSSALSQGSLSSLQTCDILSSAREQAQAKAGSGQTACGVEDVLQLLRILYIIAGDPYSTRTLQEEPDEQLQFNVSPEEFTSKKVTTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATMERSRPSTAVRRDDPGEFRVGRLKHERVKVPRGDSMMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRKSLGVWLCDDDFPDDESRQVDLGGGLKPPGYYVQRSCGLFPAPFPQDSDELDRISKLFLFLGVFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLLYESRGDADLHFSEIQSEASTEEGHDTYSVGSFDEDSKSEFILDPPKPKPPAWYHGILTWEDFELVNPHRAKFLKEVKELAVMRRQILTNKSLTEDEKNTRLQDLMLKNPSGSGPALSIEDLGLNFQFCPSSKVHGFTAVDLKPNGEDEMVTIDNAEEYVELMFDFCMHTGIQKQMESFRDGFNRVFPMEKLSSFSHEEVQMILCGNQSPSWTAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTIVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUI3</accession>
    <name>W5MUI3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysosome-associated membrane glycoprotein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytolytic granule membrane</location>
            <topology evidence="12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Late endosome membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the LAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUI3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012063.1">
        <property type="protein sequence ID" value="ENSLOCP00000012042.1"/>
        <property type="gene ID" value="ENSLOCG00000009839.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182899"/>
    <dbReference type="InParanoid" id="W5MUI3"/>
    <dbReference type="OMA" id="SSNQIHM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009839">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000001">
        <property type="entry name" value="lysosome-associated membrane glycoprotein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000026">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048528">
        <property type="entry name" value="Lamp2-like_luminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048524">
        <property type="entry name" value="Lamp2-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018134">
        <property type="entry name" value="LAMP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF27">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01299">
        <property type="entry name" value="Lamp2-like_luminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21222">
        <property type="entry name" value="Lamp2_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00336">
        <property type="entry name" value="LYSASSOCTDMP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00310">
        <property type="entry name" value="LAMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00311">
        <property type="entry name" value="LAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51407">
        <property type="entry name" value="LAMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="17">
        <location>
            <begin position="32"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="17">
        <location>
            <begin position="227"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like transmembrane" evidence="18">
        <location>
            <begin position="391"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="345"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="424" mass="44908" checksum="A117B1BF6E31D1B3" modified="2014-04-16" version="1">LLGQVGPFPAPEMIILSCLSGCLRPVHAVVLEVKEGNVSCIIADLSANFSVLYSLSSGTGTAQLVLPSSAEVDKGSRCGNTSLSPLLVASFGNGHSLSLGFFRNETKYGVGTLNVTFNLNDNVTFPDAKSKDPLNASSSSTGILAQLNTTYRCMSTSRVVMDGVNVTFTNMRVEAYVKANNLSKEVETVCAADASATTTSAPKTTPVTTTPVTTTPVPTMAPGNPLPGNYNVTNQNGTCLLAAMGLQLNFTYFSAVRNKTVQDIVNLQPNVTKSSGSCEASSATLILMYERTNLTFTFTLNSTVKKYHLSALGVDASLPDMTAPFSASNATLDVLRGTLGKSYMCNSKQTLVVSDRFSVNTFRLQVQPFGVSNNQFGAAEECQLDEDNMLIPIIVGAALAGLVLIVLIAYLIGRKRSHAGYQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWA0</accession>
    <name>W5MWA0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrator complex subunit 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWA0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012659"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012683.1">
        <property type="protein sequence ID" value="ENSLOCP00000012659.1"/>
        <property type="gene ID" value="ENSLOCG00000010329.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695689"/>
    <dbReference type="KEGG" id="loc:102695689"/>
    <dbReference type="CTD" id="26512"/>
    <dbReference type="eggNOG" id="KOG3768">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016655"/>
    <dbReference type="HOGENOM" id="CLU_006789_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWA0"/>
    <dbReference type="OMA" id="LNQNHYG"/>
    <dbReference type="OrthoDB" id="9449012at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010329">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000010">
        <property type="entry name" value="Integrator complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057413">
        <property type="entry name" value="Beta-barrel_INTS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029307">
        <property type="entry name" value="INT_SG_DDX_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051113">
        <property type="entry name" value="Integrator_subunit6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12957">
        <property type="entry name" value="DEAD/H BOX POLYPEPTIDE 26/DICE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12957:SF23">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25462">
        <property type="entry name" value="Beta-barrel_INTS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15300">
        <property type="entry name" value="INT_SG_DDX_CT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="738"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="880" mass="98897" checksum="6D16782FA19EC4E3" modified="2014-04-16" version="1">MPVLLFLIDTSASMNQRTHLGTSYLDTAKGAVETFMKLRSRDPASRGDRYMLLSFEEAASGIKAGWKENHATFMSELKNLQAVGLTTLGHSLRTAFDLLNLNRLVTGIDNYGQGRNPFFLEPAIIVTITDGNKLTSSSGVQEELHLPLNTPLPGSELTQEPFRWDQRLFALVLRIPGIAAPEPEAAAGVPQDDSAITPMCEVTGGRSYSVCSQRMLNQCLESLVQKIQSGVVINFEKTGSDPPPVEDAPVDAGIPGPQPWHCCHKLIYVRPNPKTGVPIGHWPIPEAFWPDQNSPTLPPRSAHPQVHFSCVDSELMVIDKLPFDKYELEPSPLTQFILERKSPHTCWQVFVCNSAKYSDLGYPFGYLKASTALNCVNLFVMPYNYPVLLPLLDDLFKVHKLKPTLKWRQSFENYLKTMPPYYIGPLKKALRMMGAPNLIAENMEYGLSYSVVSYLKKLSHQAKVESDRVIASLGKKVVPDMGVKVRVRPTPLSLAQRKDFQQILQGVTGDAPFCPLALNPKEFAGFHIGILHKDVKPQSFRNPYDIPRRHLLDQLSRMRKNLLKATHCCLRGQDEDQLHSIAIAQMGNYQDFLKNLPSPLREVDPDQPRRLHTFGNPFKLDKKAMMIDETDEFITGTQNKSKRPGDPSLQGTPKRRRCMSPLLRSGHLQGPLNTSNHTVEKDPSAPLSPVPSPPANLTRPTPVPRAPEKSYQVPVENDIFGNHMGDHYNVEREPVSDSDGSCFQYSQGPVNQRNHVGEDDHSSLSKLSEDSTEEEPSPEDTVCSSSPGPLKRLRHTQGQEVNAELKALITREIRKPGRRYDRIFYFLKQVQGTLNTRLVFLQNIIKEAARFKKRVLIEQLEQFLEEIHVRYSQINHVDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWT6</accession>
    <name>W5MWT6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">FAM3 metabolism regulating signaling molecule D</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202596.1">
        <property type="nucleotide sequence ID" value="XM_015347110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWT6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012845"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012871.1">
        <property type="protein sequence ID" value="ENSLOCP00000012845.1"/>
        <property type="gene ID" value="ENSLOCG00000010476.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688775"/>
    <dbReference type="KEGG" id="loc:102688775"/>
    <dbReference type="eggNOG" id="ENOG502S1XI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183004"/>
    <dbReference type="HOGENOM" id="CLU_099478_0_1_1"/>
    <dbReference type="InParanoid" id="W5MWT6"/>
    <dbReference type="OMA" id="MCFENQI"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010476">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13940">
        <property type="entry name" value="ILEI_FAM3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039220">
        <property type="entry name" value="FAM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039475">
        <property type="entry name" value="ILEI_FAM3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14592">
        <property type="entry name" value="UNCHARACTERIZED FAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="ILEI/PANDER" evidence="9">
        <location>
            <begin position="102"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="226" mass="25025" checksum="46380A00C1C78DDC" modified="2014-04-16" version="1">MRTRDAIRLILVLASVLLMWLIANMPINIQLKIKDTKDILGLLKPTPAHAVVTPANKCGLSKECPADHFPFRIKSGAANVIGPKICFDGKIVMSNALNNVGSGLNILLVNGQTGEIIKAESFNMYAGDVKGLLEFLKAIKPGTIIIVASYDEPATNMNDEIREIFTRFGSSMIKSVQYRDNWLFIGAEGIEEKSPFEKHLKNAQDTNIYDGWPEMIEMDGCFPKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY09</accession>
    <name>W5MY09_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrogenase/reductase SDR family member 7C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Short-chain dehydrogenase/reductase family 32C member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635376.1">
        <property type="nucleotide sequence ID" value="XM_006635313.3"/>
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        <property type="interactions" value="6"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000013296.1">
        <property type="protein sequence ID" value="ENSLOCP00000013268.1"/>
        <property type="gene ID" value="ENSLOCG00000010828.1"/>
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    <dbReference type="GeneID" id="102686276"/>
    <dbReference type="KEGG" id="loc:102686276"/>
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    <dbReference type="eggNOG" id="KOG1205">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157100"/>
    <dbReference type="InParanoid" id="W5MY09"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
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    <dbReference type="Bgee" id="ENSLOCG00000010828">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
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    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="CDD" id="cd05332">
        <property type="entry name" value="11beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.720:FF:000122">
        <property type="entry name" value="Dehydrogenase/reductase SDR family member 7B"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
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        <property type="entry name" value="SDR_fam"/>
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        <property type="entry name" value="SDR_family_member_7C"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44668:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7C"/>
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        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="34766" checksum="CA24FC1B0DB8644B" modified="2014-04-16" version="1">MGFTSVFILPVLVVLVAGMYYLYNEIVKFMSKSVVRNKVVVITDAVSGLGKECSRVFHMGGARLILCGKSWEKLEALYDALNSVADPSVTFTPKLVLLDLSDINGIPEVTREILECYGCVDVLISNASMKVKAPVQTLSLEMDKKIMDTNYFGPITLTKGLLPSMTSRRTGHLVLVNSIQGKLAMPFRTAYAASKHAAQAFFDCLRAEVEEYGISVSTISPTFIRSYHLQPEQANWEKNIWKFFFRKLSHGVHPVEVADEILRTVSRKKKEVLMANPVPRAALYIRSFFPGLFFAVVAAGVKDVTLVEEEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY52</accession>
    <name>W5MY52_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NOP2/Sun RNA methyltransferase 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209817.1">
        <property type="nucleotide sequence ID" value="XM_015354331.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209818.1">
        <property type="nucleotide sequence ID" value="XM_015354332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY52"/>
    <dbReference type="SMR" id="W5MY52"/>
    <dbReference type="FunCoup" id="W5MY52">
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013311"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013339.1">
        <property type="protein sequence ID" value="ENSLOCP00000013311.1"/>
        <property type="gene ID" value="ENSLOCG00000010862.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698492"/>
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    <dbReference type="eggNOG" id="KOG1122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155370"/>
    <dbReference type="HOGENOM" id="CLU_005316_1_1_1"/>
    <dbReference type="InParanoid" id="W5MY52"/>
    <dbReference type="OMA" id="YQGAMLY"/>
    <dbReference type="OrthoDB" id="260824at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010862">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21150">
        <property type="entry name" value="PUA_NSun6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="154"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="255"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="483" mass="53201" checksum="AF9E3BB76DCF916B" modified="2014-04-16" version="1">MHCTVHEEMSVFPKITLRGDVKQYLGDVFKNKELLAVLGQEDAERRFEMLLSRLSHPPAFTSVRVNTHLASPEHVRRCLSEEIRKQQQMNSFSSHDVPIFNHPDVPDVLLIPVIGPRVSLERHSLEVVVGAQCGNAVLRGAHVFAPGILAAPKYMKMGDVVSVFSDIEGQCTRGAKEFQGKRVFIGNGIAEMSRSDIFCTDQPIKGIGIRMTEPVYFSPSFYNVLPNQVFLQNLPSAIVSHVLQPQAGERILDMCAAPGGKTTHIAALMGDEGEVIAMDKIANKVERIKQNAQMLGLNSIKVYCFNAIKALSGDPAGSSGGGPPFPPESFDRVLLDAPCSGLGQRPNMACTWSLKEITSYQPLQRQLFTTAVKLLKRGGVLVYSTCTITLAENEEQVAWALHHFPFLSLQRQEPHVGGGGMLGAGLSHDHLKLLQRFNPVWATTEGFTLHPEEYEHTQGTKSDLIHLANKDTIGFFIAKFVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZS2</accession>
    <name>W5MZS2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAU ubiquitin like and ribosomal protein S30 fusion a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZS2"/>
    <dbReference type="FunCoup" id="W5MZS2">
        <property type="interactions" value="1149"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013881"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013910.1">
        <property type="protein sequence ID" value="ENSLOCP00000013881.1"/>
        <property type="gene ID" value="ENSLOCG00000011291.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007479"/>
    <dbReference type="HOGENOM" id="CLU_010412_5_0_1"/>
    <dbReference type="InParanoid" id="W5MZS2"/>
    <dbReference type="OMA" id="FRRIQYT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011291">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01793">
        <property type="entry name" value="Ubl_FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000114">
        <property type="entry name" value="40S ribosomal protein S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039415">
        <property type="entry name" value="FUBI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF30">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="144" mass="15893" checksum="87FCACA63DBA2C1A" modified="2014-04-16" version="1">VSVTSSLCRVKMQLFVRTQNTHTLEVTGQETVRDIKARVQALEGLCVEEQVLLLAGAPLEDEAVLEQCGLTEHCTLEVAARMLGGKVHGSLARAGKVRGQTPKVDKQEKKKKKTGRAKRRIQYNRRFVNVVPTFGKKKGPNANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZT2</accession>
    <name>W5MZT2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">triacylglycerol lipase</fullName>
            <ecNumber evidence="4">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01017153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015195677.1">
        <property type="nucleotide sequence ID" value="XM_015340191.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZT2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013891"/>
    <dbReference type="ESTHER" id="lepoc-w5mzt2">
        <property type="family name" value="Lipoprotein_Lipase"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000013920.1">
        <property type="protein sequence ID" value="ENSLOCP00000013891.1"/>
        <property type="gene ID" value="ENSLOCG00000011301.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693403"/>
    <dbReference type="KEGG" id="loc:102693403"/>
    <dbReference type="CTD" id="9388"/>
    <dbReference type="eggNOG" id="ENOG502QU8P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159394"/>
    <dbReference type="HOGENOM" id="CLU_027171_1_0_1"/>
    <dbReference type="InParanoid" id="W5MZT2"/>
    <dbReference type="OMA" id="QMPVGHV"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011301">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000010">
        <property type="entry name" value="hepatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000109">
        <property type="entry name" value="Lipase, endothelial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002330">
        <property type="entry name" value="Lipo_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF13">
        <property type="entry name" value="ENDOTHELIAL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00822">
        <property type="entry name" value="LIPOLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868550" description="triacylglycerol lipase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="18">
        <location>
            <begin position="349"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="497" mass="55961" checksum="22DE8D2EF40E9450" modified="2014-04-16" version="1">MKNTVIPLWILVLSSVVLASDLEKEGLFKDGAIADLLKDENGSHDTGNPQVKFNMRTSLGLDDEGCFIHLGKASLQECGFNATAKTFFVIHGWTMSGMFESWLYDLVFALQKREQDANVIVVDWLTLAHRLYPDAVNNTRIVGHDIAKLLEWLKKELTLPLENVHMIGYSLGAHVAGFAGTFVQGTVGRITGLDPAGPMFEGVESDHRLSPDDADFVDVLHTYTREALGVSIGIQQPVGHIDIYPNGGDFQPGCGLSEMLSTMAYGGIGDVVKCEHERSVHLFVDSLLNKDHKSFAYQCTDPGRFKKGICLSCRKNRCNNLGYNAKKMRKRRSCKMYLKTRADTPFGGYHYQMKMHVFSKTDAEDIDPTLHVKLYGASNDSEDILVELPGKLGLNFTNTFLAFTEEDIGDLLKIKLTWEDTSKSWSTFFKSWLTWRNPAQDQVLMVRRIRVKCGETQKKFTFCAQDPDLTKISPGNEINFLKCRDGWEVKPRKRLHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0B2</accession>
    <name>W5N0B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 652</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0B2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014071"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014100.1">
        <property type="protein sequence ID" value="ENSLOCP00000014071.1"/>
        <property type="gene ID" value="ENSLOCG00000011452.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157416"/>
    <dbReference type="HOGENOM" id="CLU_002678_74_2_1"/>
    <dbReference type="InParanoid" id="W5N0B2"/>
    <dbReference type="OMA" id="VESCAVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011452">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000900">
        <property type="entry name" value="Zinc finger and BTB domain containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000312">
        <property type="entry name" value="Zinc finger and BTB domain-containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000550">
        <property type="entry name" value="Zinc finger and BTB domain-containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000166">
        <property type="entry name" value="Zinc finger and BTB domain-containing 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001300">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001378">
        <property type="entry name" value="Zinc finger protein 652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000284">
        <property type="entry name" value="Zinc finger protein 652 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF15">
        <property type="entry name" value="IP01243P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="546"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="605"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="668" mass="74393" checksum="AACB23E1D9B945CA" modified="2014-04-16" version="1">TPAPGRPVMKSCQKPKEGLAMQSATGVSPEDSRRLQAPPSYYHAPGQELDLSTKLFKREGGGKAYSVLVDTELSKRHLAERGGQQAQAPQGQQQPYYREGGKGAPEGGGQASAGEGEGSEETEEEEEEEEEEEVEESFKREQIIVEVNLNNQTLNVSKGDKGVASQSKNVAPVIKSSSEEDEEEEEEEEASESPEEDQEEEENERQKEKAKKPRRAPAASQPPRKTRKAAMAAASAVASCVTTRGRRKSVEPPKRKRRAPREAKGSSPGGAATGKSAGEERETLTCEKCPRVFNTRWYLEKHMNVTHRRMQICDKCGKKFVLESELALHQQTDCEKNIQCVSCNKSFKKLWSLHEHIKIVHGYAEKKFTCEICEKKFYTMAHVRKHMVAHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFRCENCDERFQYKYQLRSHMSIHIGHKQFMCQWCGKDFNMKQYFDEHMKTHTGPGEKPFICEICGKSFTSRPNMKRHRRTHTGEKPYPCEVCGQRFRFSNMLKAHKEKCFRVTSPVNVPPALPLPLTSSSSGAAAQAPPPAPPPASSPSPTSPPPGAPLGLSPVSAPPPRPLGHPFSHLHLHPPPPPPPPPPPPPHHHLPVPPVSHLPPPPALFKSEPQLNHRGHGEDSYLRHLADKNSGAPHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0B6</accession>
    <name>W5N0B6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0B6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014075"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014104.1">
        <property type="protein sequence ID" value="ENSLOCP00000014075.1"/>
        <property type="gene ID" value="ENSLOCG00000011456.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV2W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163253"/>
    <dbReference type="HOGENOM" id="CLU_026010_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0B6"/>
    <dbReference type="OMA" id="MGCCVDL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011456">
        <property type="expression patterns" value="Expressed in ovary and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF117">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="P-type" evidence="15">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="14">
        <location>
            <begin position="141"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49685" checksum="20ED7B08D81B8C7E" modified="2014-04-16" version="1">MTVQLPPGPLAEVHILDSSKTLVPVLQTPRTCGYSLVKGEGKNILTIPYTACDVKIVGRRYTIQVAYTSGGQRAEIQVSCPYRRLKPKQGESMPLPSPPGCHIPRSQQVSCGPRRMGSSQCLAKGCCVDPETAHCYYPLEECTSDRHFVFAVHRTVTVPHVDPASLVISGNKSCAPVICTPDFAVFKFPVTGCGTHAFVVGETTIYLAEVMAMARRNSLNYGVITRDSPFRLLVECRYAEGNLASTGYLVKSPYLPSAILSQGVFGVQLRIATGKRVGTCSCPDEHYSRFYPQYHRPLRLLLGSPVYLEVRLLNAPDPSLVLLVHYCIAYPRSAQAAWVLLYEGCPNPLDYGQTSTLHINDKVPLPKHHRRFEIRTFQFMERTAHRYLDEEIYFMCSTEVCSPKVRKCVEGCFDGRKIPVIPDPNLDKRCGRKPCPVNSKSSELPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0Z7</accession>
    <name>W5N0Z7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ena/VASP-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ena/vasodilator-stimulated phosphoprotein-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206001.1">
        <property type="nucleotide sequence ID" value="XM_015350515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0Z7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014306"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014335.1">
        <property type="protein sequence ID" value="ENSLOCP00000014306.1"/>
        <property type="gene ID" value="ENSLOCG00000011645.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682773"/>
    <dbReference type="CTD" id="58003"/>
    <dbReference type="eggNOG" id="KOG4590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157826"/>
    <dbReference type="HOGENOM" id="CLU_017790_1_1_1"/>
    <dbReference type="InParanoid" id="W5N0Z7"/>
    <dbReference type="OMA" id="APCYESH"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011645">
        <property type="expression patterns" value="Expressed in muscle tissue and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000004">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000071">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF4">
        <property type="entry name" value="ENA_VASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="WH1" evidence="13">
        <location>
            <begin position="15"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="158"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="429"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="699" mass="74136" checksum="B8A216A1E2C41F48" modified="2014-04-16" version="1">MYSFDEFGCRQTAQFACFCEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTANNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNVLSQDAGPAVQRQVQNGPSADDMDMQRSRQMMEQQQLQAQIERDRRASTSVSTLQIKVSPSSSRSQSPPPEYSHCRAGAMPPPSYAKVTSSSPSSRDGEGGCASLQADGTSDSPQRKKPVASQLSTSLASAFSPVQPGVGTLSRNVRQIPISPPLAASQAPLLPPKSGAWSSPHMFSPLPPSPPVVMALAPLKQVCPQPMLPVAHPLLPVHNGRGKGPADLSLFQHGAEYSSDHLEDYCLNQHQMHCSSGSASHPVLYKHPDGSFAPCYESHPLPTLIGHPVTGPTHQSQFPSYHLQQLYPSVPPISSSPPSYTALSEGTLPKKTPPSPSQAAVPHSSPPLPPGHPSSVLSVAPPTAAPASGGGPPPPPPPPGPPPPSAGAPPPPPPLPAGGGSGGAGDESPGSGLAAALAGAKLRKVQRSEDASGGTSPGGAAKNDANRTSGGGGLMQEMNALLARRRKAATQTEKSSDKKEEESQNEDSSLSPSPVTRGPGQQNSTDPLKKPWDRANSADKSSLVSRVKPVGSTNDMPADALDFDRMKQEILEEVVRELHKVKDEIIDAIRQELSRISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1Y6</accession>
    <name>W5N1Y6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">F-box/LRR-repeat protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">F-box and leucine-rich repeat protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. During mitosis, it mediates the ubiquitination and subsequent proteasomal degradation of AURKA, causing mitotic arrest. It also regulates mitochondrial function by mediating the ubiquitination and proteasomal degradation of the apoptosis inhibitor BIRC5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXL7) composed of CUL1, SKP1, RBX1 and FBXL7. Interacts with AURKA; interaction takes place during mitosis but not in interphase. Interacts with BIRC5; this interaction allows BIRC5 to be polyubiquitinated by the SCF(FBXL7) E3 ubiquitin-protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the FBXL7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031769">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031770">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031771">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031774">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634450.2">
        <property type="nucleotide sequence ID" value="XM_006634387.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1Y6"/>
    <dbReference type="FunCoup" id="W5N1Y6">
        <property type="interactions" value="543"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014645"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014674.1">
        <property type="protein sequence ID" value="ENSLOCP00000014645.1"/>
        <property type="gene ID" value="ENSLOCG00000011912.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690339"/>
    <dbReference type="KEGG" id="loc:102690339"/>
    <dbReference type="CTD" id="23194"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158009"/>
    <dbReference type="HOGENOM" id="CLU_016072_5_0_1"/>
    <dbReference type="InParanoid" id="W5N1Y6"/>
    <dbReference type="OMA" id="ETVHVDR"/>
    <dbReference type="OrthoDB" id="423607at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011912">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22120">
        <property type="entry name" value="F-box_FBXL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000122">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000126">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000018">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF50">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="17">
        <location>
            <begin position="111"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="491" mass="54585" checksum="C6CA82745C9B2F59" modified="2014-04-16" version="1">MGANNGKNYGSEGKGSSSISSDVSSSTDHTPTKAPKNAATSEDSDLSMRTLSTPSPALICPPSSQACINGKDSSTSSSSVTGETIAMVHAPPTTCLPHPHIRPASRQQKEQAPIDILPDHSFIQIFSHLPTNQLCRCARVCRRWYNLAWDPRLWRTIRLTGDMVNVDRALRILTRRLCQDTPNVCLMLETVIVSGCRRLTDRGLYTIAQCCPELRRLEVSGCYNISNEAVFDIVSRCPNLEYLDVSGCSKVTCISLTREVSVKLSPLHGKQISIRYLDMTDCFVLEDEGLHTIAAHCTQLTHLYLRRCVRITDEGLRYLVIHCGAIKELSVSDCRFVSDFGLREIAKLESRLRYLSIAHCGRITDVGIRYVAKYCTKLRYLNARGCEGITDHGLEYLAKNCAKLKSLDIGKCPLVSDGGLEFLALNCFNLKRLSLKSCESITGRGLQIVAANCFDLQLLNVQDCEISVEALRFVKRHCKRCVIEHTNPAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2E0</accession>
    <name>W5N2E0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
            <ecNumber evidence="10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EC" id="3.1.3.48" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01005457">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631995.1">
        <property type="nucleotide sequence ID" value="XM_006631932.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2E0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014799"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014828.1">
        <property type="protein sequence ID" value="ENSLOCP00000014799.1"/>
        <property type="gene ID" value="ENSLOCG00000012031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688449"/>
    <dbReference type="KEGG" id="loc:102688449"/>
    <dbReference type="CTD" id="1843"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159044"/>
    <dbReference type="HOGENOM" id="CLU_027074_0_2_1"/>
    <dbReference type="InParanoid" id="W5N2E0"/>
    <dbReference type="OMA" id="MVNMQVC"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012031">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903753">
        <property type="term" value="P:negative regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14638">
        <property type="entry name" value="DSP_DUSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000015">
        <property type="entry name" value="Dual specificity phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000026">
        <property type="entry name" value="Dual specificity protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF309">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="32"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="186"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="251"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="40622" checksum="9872A667C9131D97" modified="2014-04-16" version="1">MRLDLPFSTCRPTTMVIMEVPSLDCAAFRNLLDAGALVLDCRSFFSFNSSHIPGSTNVRFSTIVRRRARGGLGLEHIVPNEEARCRLLSGQCRAVVFLDERSADFEQVKKDSTLMLAVAALRRDPCGASVFLLRGGFEAFASEFPELCTKPAAPQGLCLPLSSSCPPGSAESGCSSCGTPLYDQGGPVEILPFLYLGSAYHASRKDMLDTLGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIEFIDSVRNSGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSVISPNFSFMGQLLQFESQVLAPSSCSAEAGSPALGNTGTVFNFPVSIPVCSSSSPLTFLHSPITTSPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2P6</accession>
    <name>W5N2P6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein Spindly</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 99</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Spindle apparatus coiled-coil domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SPDL1</name>
        <name evidence="8" type="synonym">CCDC99</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Centromere</location>
            <location evidence="8">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Spindly family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2P6"/>
    <dbReference type="FunCoup" id="W5N2P6">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014905"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014934.1">
        <property type="protein sequence ID" value="ENSLOCP00000014905.1"/>
        <property type="gene ID" value="ENSLOCG00000012111.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S27G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047951"/>
    <dbReference type="HOGENOM" id="CLU_031713_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2P6"/>
    <dbReference type="OMA" id="KQHAFTK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012111">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03041">
        <property type="entry name" value="SPDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028593">
        <property type="entry name" value="SPDLY_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF9">
        <property type="entry name" value="PROTEIN SPINDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 8" id="KW-0137">Centromere</keyword>
    <keyword evidence="1 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="3 8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="569"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="102"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="617" mass="70516" checksum="41EFCC05717CF7DD" modified="2014-04-16" version="1">MSAAVDSEVQRLRCKLKEAEESLQKAAQYGLQLLDDQLDLQNRLEEQRTEMTNSIEALEQEKYSLQRELELRGRMLDSLRSECESIKTQQKMLWVQQEAQLERSYTLEIGEYKNKLEKMKAELDEARLSERQLRHKLELQAEALSSKTEELRMMAERSQETLSSEMMELQMEKAELETAKAALEHELNEMSYREQQLQLAGSHLQRTVERLTREKEDCEKEVVSHFNALEEPVRACSFINRTWDMLLMRMQDPNQLLHLCQTVLLQDTPDMQAHLTVTAALLQYSSSDESQSDESLNSFRGQVQIATLMQMKGCQADPGQLERLQSMLAQKNNEIEALMIKLRRLERAEMTIKAEPSSAPSGEGDSGDMTYYTDLLKMQLSNSVKEAERLGEELSVQRMKALAESQRVLEVERRLFSSERTLRLCQSENIRLQVQLDELRMKYQPNAVNKSQVAKRRREKLPVDVPAEADAPEPKVSTAQFFPDQAHGRLCVIVTTSQLLHATCFVYTTSASLKKQETDKILVLCEECPTQPQICTAKRVQAFASEHTSCAFCLSNTASCIFCTSSNMSPPSGTTVKAEEVDAGAENRREERKKEMRPQPVIHVSSKPTLENQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2Z0</accession>
    <name>W5N2Z0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <sequence length="333" mass="37533" checksum="2101E68CD64089B1" modified="2014-04-16" version="1">SFLLFLFSRRLVAWNCTINCSCNGLSSTCCHPHPALIFPITGHPNTTVLLLDSCSHFRLNRETLQNFAALENISIKRTYIAYIDSDAFHHIPNLRTLILEETNLSNTAIHHNAFHKLRITVLSLRSNRLVRIGHQTFSGLINLQHLDLSGNNIAVIEDSSFTALVNLQFLNLDNNSLNTITSLWFSGASLNNTNLKISLLANNLTCECRYRGIDLPEYAWFKRSLILPNKSCLLYNTKEECKPPKLEEFYQVIHGIALQPLDLHCPVTGFPFPKIMWILPNGLQISNRSFPPFYNIDGTLTIQLVHSSFGGLYACMATNIEGTEIGLYNLVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="entry name" value="WWC1_hairpin"/>
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        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN KIBRA"/>
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        </source>
    </evidence>
    <sequence length="1133" mass="125896" checksum="5CAF8DE774CF972A" modified="2014-04-16" version="1">MPRKELPLPQGWEEARDFDGRVYYIDHINKTTSWIDPRDRQTKPLTFADCIGDELPVGWEEAYDPKVGAYYVDHNTKTTQLEDPRAQWQWEQENMLREYLEVAQDALNAQREIYQVKEQRLKLAQQEYRQLNEVWKDKSTSRTSLNSGSSSSSKYDPEILKAEIVTAKSRVNKLKRDLAHMRQELQYKEQGYETLKEIDQKVSQAPCGYKLHEAQAILNEVKSIKDAISLGEKEKKELMQKLAILKDGFRLTSGSQSDLWDSTSSLADSSLSIPHLYSDAESQTDLPGDLALSSNNKLAEKVRLSLKYEEAKRRIANIEVQIAKLDSEAWPGLLDPERDRLILISEKEELLKELQYVRPRRREPDDVAKIETEKKRLERDLQAARDNQSKALTERLKLHSRRNKLVKELEETARLASSLHSQLKSLSASTLSCSSGSSRGSLASSRGSLAASSPGSSSSLSFTDLYGDPPEPGDPDFQGRLDCVLQEGAAGFRPSSSITTIHENEVIRTPGAAPGPPRGQVPRLSETPRSMSSLSPRSSFSSLSPPCSPLLRDPTFLGGEAFLGHGGAGLDLELQGRLAELQLDGSGQPSAEGQHAPPPRSAAQELHATQGSTTAGAEAGRDGSAPGRGPGPVLKRPGVSSAVSDESVAGDSGVYEPSERRPTRPADVVLNSDEGGSIGTAQIQLGMKYDARERRFAVFVVQLCNASVLPLPLDEKIYVRVAVLPCPQTTSCLFRTRASIPQDKLEFSEVFGVSVSLGALRQKTLRVDVCYIKTSGREECLAGAQISLAEVSSSGEGSTQWYNLLNNKCLAEAGTEHRGAEDSPRRAEGPVSEPVAEITERPDDDKNAFISEEEWTSEHLEGEFWEEGEGSDAEAAEVQEDEESYPETSRRAGEEGTAGQAYTGRITERANKETSTDSLAQAASVVRPKERRAAAPQQTPLVRGNTIIRSKTFSPGAQSQYVCRINRSDSDSSTLSKRSPFVRNASERRSVRVKRPVLQARGLDGLLRTSLDMELDLQVSKTKHSRLNQELEVLRQLKEQLEQARAQGRSELPAWVQQDERFRLLLKQAEKQTEEEQQQELRVERMMRTAAKDVHRIRGQSWKEAREVHSFREKMAFFTRAKITIPDLPADDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N400</accession>
    <name>W5N400_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01023099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023100">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N400"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015359"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015388.1">
        <property type="protein sequence ID" value="ENSLOCP00000015359.1"/>
        <property type="gene ID" value="ENSLOCG00000012466.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156225"/>
    <dbReference type="InParanoid" id="W5N400"/>
    <dbReference type="OMA" id="GSCPNNV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012466">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005025">
        <property type="term" value="F:transforming growth factor beta receptor activity, type I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23612">
        <property type="entry name" value="TFP_LU_ECD_BMPR1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF50">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN RECEPTOR TYPE-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047511562" description="receptor protein serine/threonine kinase" evidence="22">
        <location>
            <begin position="17"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="24">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="235"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="533" mass="60139" checksum="D2F9F986A195FF80" modified="2025-10-08" version="2">MYVFFLFCSAWGFSLAKHRSCSTLGGHNPDHVLHGTGAKPGSNPKKAVDGSTVSPEDAARFLSCYCSGHCPEDAKNNTCETNGQCFAIIEEDEHGEAILTSGCMKYEGSDFQCKDSPRAQSRRTIECCRTDFCNRDLQPTLPPVIDKGPFFGSTHWLAFLISVTVCCCALIAITIICYYRYKWQTERRRYNRDLEQDEAFIPVGESLKDLIDQSQSSGSGSGLPLLVQRTIAKQIQMSRQIGKGRYGEVWLGRWRGEKVAVKVFFSREEASWFRETEIYQTVLMRHENILGFIAADIKGSGAFTQLFLITDYHENGSLYDYLKYTTLDTKSLLKLAYSAACGLCHLHTEICGTQGKPAIAHRDLKSKNILMKKNGTCCIADLGLAVKFNSDTNEVDIPLNTRVGTKRYMAPEVLDETLNKNHFQSYIMADIYSYGLIIWEMARRCVTGGIVEEYQLPYYDMVSADPSYEDMREVVCVKCLRPAVSNRWNSDECLRAMLKLMSECWAHNPPSRLTALRVKKTLAKMVESQDIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N444</accession>
    <name>W5N444_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N444"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015432.1">
        <property type="protein sequence ID" value="ENSLOCP00000015403.1"/>
        <property type="gene ID" value="ENSLOCG00000012513.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SNSD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160789"/>
    <dbReference type="HOGENOM" id="CLU_062834_1_1_1"/>
    <dbReference type="InParanoid" id="W5N444"/>
    <dbReference type="OMA" id="LICFARF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012513">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032197">
        <property type="term" value="P:retrotransposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="31528" checksum="C2506BC532BEB7E8" modified="2014-04-16" version="1">DASLQKILYELRDFRWDNKEQITDIKLELSNVNKRLDKAEGRTEEVETRVLRTETVIKKLLQRQVTLETKLTDQEGRLRRENIRIYGIPEESEGCCMVTFLENLRRGELDLPKDMELGIERAHRGVAKFLSYKMKEEVLGKAWQMKQTILCYHDNTPTVLKKRMEYSEVKKVLKSQKIKFQTFPARLQVFYEDSTCLYHSAAEATKDMVEGGLKVQVIPPAPTLEEEIERLFTWQKAGRRGGLRKDQSSKQQYKEKLQSFRRRPPSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5N587</accession>
    <name>W5N587_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin-10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cadherin-6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634604.1">
        <property type="nucleotide sequence ID" value="XM_006634541.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N587"/>
    <dbReference type="FunCoup" id="W5N587">
        <property type="interactions" value="347"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015796"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015826.1">
        <property type="protein sequence ID" value="ENSLOCP00000015796.1"/>
        <property type="gene ID" value="ENSLOCG00000012835.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686680"/>
    <dbReference type="KEGG" id="loc:102686680"/>
    <dbReference type="CTD" id="568370"/>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154187"/>
    <dbReference type="HOGENOM" id="CLU_005284_3_1_1"/>
    <dbReference type="InParanoid" id="W5N587"/>
    <dbReference type="OMA" id="RQDLHRS"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012835">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000006">
        <property type="entry name" value="Cadherin-9 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF322">
        <property type="entry name" value="CADHERIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867207" description="Cadherin-10" evidence="19">
        <location>
            <begin position="22"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="78"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="159"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="268"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="383"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="487"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="786" mass="87854" checksum="D46C20755AEFBB7F" modified="2014-04-16" version="1">MVFNHSLLLLLLLSFHHQSYMMVLSQRNNDETLHKLTGASDKDGKILQRSKRGWMWNHFFLLEEYTGTEYQYVGKLHSDQDRGDGSVKYVLSGEGAGTLFLINENSGDIQATKKLDREEKAYYTLRAQAVNKKTGRPVEPESEFIIKIHDINDNEPKFTKETYLASVPEMSDVGTFVVQVTATDADDATYGNSAKLVYSILQGQPYFSVEPETGIIRTALPNMNRENKENYHVMIQAKDMAGQMGGLSGTTTVNITLTDVNDSPPRFPQSSYHLSTSESAEIGATVGRIKANDADVGENADIQYNIVSGDGQDLFKIVTDRSTQEGVITVKRQLDFENKRIYLLKVEAVNTHLDHRFLHLGPFKDTATVKISLQDMDEPPVFSKSFYVFEVHEDAAPGSFIGAITAYDPDAANHPVKYSVDRHTDLDRLFDIDSGNGSISTLQPLDREMSKWQNISIVATEINNPRLTSRVPVFIKVLDVNDNAPEFAMFYETFVCENVKSGQLIQTISAVDSDEPLIGHKFAFSLSTTNPNFTIFDNEDNTARILTRRSGFSRREMSYYLLPVVISDNDYPIQSSTSTLTIRVCACDSRGNMQSCNAEALMLPAGLSTGALVAILLCIIILLIIVVLFAALRRQKKKEPLIISKEDVRDNIVSYNDEGGGEEDTQAFDIGTLRNPEVMDANKLRRDIIPEMLFPFRRTSPIKDNADVRDFINSRLQDNDMDPTAPPYDSLATYAFEGNGSIAESLSSLESAATDGDQDYDYLSNWGPQFKKLADMYIGKDTDKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6Y1</accession>
    <name>W5N6Y1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Gliomedin</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026099">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6Y1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016390"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016420.1">
        <property type="protein sequence ID" value="ENSLOCP00000016390.1"/>
        <property type="gene ID" value="ENSLOCG00000013299.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3545">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158020"/>
    <dbReference type="HOGENOM" id="CLU_035236_3_0_1"/>
    <dbReference type="InParanoid" id="W5N6Y1"/>
    <dbReference type="OMA" id="YIYDHGY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013299">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF85">
        <property type="entry name" value="GLIOMEDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="6">
        <location>
            <begin position="264"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="510" mass="56494" checksum="0D7A200D320A110D" modified="2014-04-16" version="1">SWKLRVLFCGLGVLTMLNCAGMIFLILQHGRLSGELADLGARLQEFSKSSVVEFLSEVTRDQAEDAGQYQYSRNKRSQELRETHIRAEQEDMLMMMTYSMVPVRVLVDLCNSTKGICLTGMYQNPLEGYLTTSRGRNSPAPRDSQPALACDPMSQARTGEQCDKGDSGEKGEKGDPGPKGEMGETYNDVLSEVSKGPPGPVGPPGPPGPVGPSGPPGPPGPPGNRSHRARAQKNSISLSYAVPNDDTLAGKAPEKAKAAPLKLECIIKFVSCPTDITKMETTFGTWMQDTASENDERIWVAEHFSGRAVKQYKNIASLQSNNSLTLDIKKFYQGCGHIVHNGSLYYHVGGTDKIAKYEISSKKLWTTKIENALFHNLTYLFHNSKTYFNLAADENGLWIIFASHSDENIMVAKLEEKSFSVVQYINTTYPRSKAGNAFIACGVLYVTDTKDMRVTYAFDLLKEKPVNVNFDLRSSNGVLAMLSYNPKNHRLYTWDNSYVKAYNVHFLSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6Y9</accession>
    <name>W5N6Y9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Distal-less homeobox 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6Y9"/>
    <dbReference type="FunCoup" id="W5N6Y9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016398"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016428.1">
        <property type="protein sequence ID" value="ENSLOCP00000016398.1"/>
        <property type="gene ID" value="ENSLOCG00000013307.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0850">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165079"/>
    <dbReference type="HOGENOM" id="CLU_074733_2_0_1"/>
    <dbReference type="InParanoid" id="W5N6Y9"/>
    <dbReference type="OMA" id="GHTRIEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013307">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000266">
        <property type="entry name" value="Distal-less homeobox 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF86">
        <property type="entry name" value="DISTAL-LESS HOMEOBOX 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="131"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="133"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="259" mass="28607" checksum="0FBE8C16829B07AD" modified="2014-04-16" version="1">RSSSLWTRRPMMTMSSMSDSLIASDPSKSAFLEFGHGLPAHQQHNHYPVHGLHSGGHPQHDGPFSPAASSYGRSLGYPYPSAVGTHHSSAYLSYQPSGHSGALGHTRIEEADHEKSTVIENGEIRLNGKGKKIRKPRTIYSSLQLQALNQRFQQTQYLALPERADLAAKLGLTQTQVKIWFQNKRSKYKKIMKHGSNGPEGEHLHPAASMSPCSPGLPPLWDVSMGSKGAPVHASSYMNSFGHWYPAHHQDSMPRPQMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8G7</accession>
    <name>W5N8G7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5N8G7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016926"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016956.1">
        <property type="protein sequence ID" value="ENSLOCP00000016926.1"/>
        <property type="gene ID" value="ENSLOCG00000013719.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687756"/>
    <dbReference type="KEGG" id="loc:102687756"/>
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    <dbReference type="eggNOG" id="KOG2452">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000160913"/>
    <dbReference type="HOGENOM" id="CLU_014974_0_0_1"/>
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    <dbReference type="OMA" id="HEEGMIL"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
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    <dbReference type="Bgee" id="ENSLOCG00000013719">
        <property type="expression patterns" value="Expressed in liver and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08647">
        <property type="entry name" value="FMT_core_FDH_N"/>
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        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
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        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
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        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
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        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
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        <property type="entry name" value="ACP-like"/>
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        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
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        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
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        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
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        <property type="entry name" value="ACP-like_sf"/>
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        <property type="entry name" value="Ald_DH/histidinol_DH"/>
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        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
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        <property type="entry name" value="Ald_DH_CS_GLU"/>
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        <property type="entry name" value="Ald_DH_N"/>
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        <property type="entry name" value="Aldehyde_DH_dom"/>
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        <property type="entry name" value="Formyl_trans_C"/>
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        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
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        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
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        <property type="entry name" value="GART_AS"/>
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        <property type="entry name" value="PP-bd_ACP"/>
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    </dbReference>
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        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="CARRIER"/>
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    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
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    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="318"/>
            <end position="395"/>
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            <position position="106"/>
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        <location>
            <position position="673"/>
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    <feature type="active site" description="Proton acceptor" evidence="10">
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            <position position="673"/>
        </location>
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    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="707"/>
        </location>
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        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
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        <location>
            <position position="142"/>
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        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
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        <location>
            <begin position="571"/>
            <end position="573"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
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        <location>
            <begin position="597"/>
            <end position="600"/>
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            <name>NADP(+)</name>
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        <location>
            <begin position="630"/>
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        <location>
            <begin position="650"/>
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        <location>
            <position position="757"/>
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            <begin position="804"/>
            <end position="806"/>
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    <sequence length="902" mass="99499" checksum="08BCD3531B7C3F3B" modified="2014-04-16" version="1">MKIAVIGQSLFGQEVYKELVKEGHQIVGVFTIPDKDGKADPLATEAEKDGVPVFKFPRWRLKGKAIPEVVDQYKAVGAELNVMPFCSQFIPMEVIDFPKHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFTLFWADDGLDTGPILLQRECNVEPNDTVNTIYNRFLFPEGVKGTVEAVRLIEQGQAPRITQPEDGATYEGIQKKENAKINWDQPAEVLHNWIRGNDKVPGAWAEVDGQKVTFYGSSMMDTTTVPDGQSLEIPGASKPGLVTKNGLVLFGNDGKMLLVKNLQFDDGKMIAASQYFHSGDSASVELTDQEKRFAEELRAIWKGILTNVDVIEHSTDFFKSGAASMDVVRLVEEVKLKGAGLQIQNEDVYMATTFQEFVQMLVRKLRGEDAEGELQVDYVEMNVNNMTVKMPHQLFINGQFVDAEGGKTYQTINPTDGTAICNVSLAQVSDVDKAVAAAKEAFENGEWGKMNARDRGRLIYRLADLMEENQEELATIEAIDSGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINQARPNRNLTFTRREPIGVCGIVIPWNYPLMMLAWKAAACLAAGNTVVLKPAQVTPLTAIKFAELTARAGFPRGVINILPGAGSLVGQRLSDHPDVRKIGFTGSTPIGKQIMKSCAVSNVKKVSLELGGKSPLIIFSDCDMDKAVRMGMSSVFFNKGENCIAAGRLFVEESIHDAFVRRVVEEVKKMKIGDPLDRSTDHGPQNHKAHLEKLIQYSQTGVKEGAHLVYGGKQVDRPGFFFEPTIFTNVEDHMYIAKEESFGPIMIISRFEDGDIDGVLKRANDTEFGLASGVFTKDINKALYVSEKLQAGTVFINTYNKTDVASPFGGFKQSGFGKDLGEEALNEYLKTKAVTIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8R9</accession>
    <name>W5N8R9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein bicaudal D homolog 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BicD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5N8R9"/>
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        <property type="gene ID" value="ENSLOCG00000013809.1"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:dynein complex binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="BICD"/>
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        <property type="entry name" value="BICAUDAL D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BICAUDAL D HOMOLOG 2-LIKE"/>
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    </dbReference>
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        <property type="entry name" value="BicD"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="190" mass="21545" checksum="140B2C16D78793BF" modified="2014-04-16" version="1">MLQEGEAEAELQRLARELQEANEEKLQAARYGLAVLEESAALKQRYAELEGEHEALRQELQQLKEALADSVSSHKRAAADGESREETLLRETASREAALAERIEELQAELRQARVTLANAQAEGDRLAALSAQLKKEGERLEAEKAQLRDELREYKVRELRQLQDSSELEEENISLQKQVSVLKENQVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9I6</accession>
    <name>W5N9I6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nucleoporin 210</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP210 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N9I6">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017295"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017326.1">
        <property type="protein sequence ID" value="ENSLOCP00000017295.1"/>
        <property type="gene ID" value="ENSLOCG00000014031.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1833">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009491"/>
    <dbReference type="HOGENOM" id="CLU_001205_1_0_1"/>
    <dbReference type="InParanoid" id="W5N9I6"/>
    <dbReference type="OMA" id="GQRFHAT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014031">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1080:FF:000007">
        <property type="entry name" value="Nuclear Pore complex Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058779">
        <property type="entry name" value="Ig_NUP210_13th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055096">
        <property type="entry name" value="Ig_NUP210_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055097">
        <property type="entry name" value="Ig_NUP210_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055098">
        <property type="entry name" value="Ig_NUP210_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056897">
        <property type="entry name" value="Ig_NUP210_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056898">
        <property type="entry name" value="Ig_NUP210_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055099">
        <property type="entry name" value="Ig_NUP210_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056899">
        <property type="entry name" value="Ig_NUP210_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045197">
        <property type="entry name" value="NUP210-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019:SF2">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23019">
        <property type="entry name" value="NUCLEAR PORE MEMBRANE GLYCOPROTEIN GP210-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26181">
        <property type="entry name" value="Ig_NUP210_13th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22967">
        <property type="entry name" value="Ig_NUP210_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22969">
        <property type="entry name" value="Ig_NUP210_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22963">
        <property type="entry name" value="Ig_NUP210_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24991">
        <property type="entry name" value="Ig_NUP210_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26182">
        <property type="entry name" value="Ig_NUP210_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24935">
        <property type="entry name" value="Ig_NUP210_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22962">
        <property type="entry name" value="Ig_NUP210_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26184">
        <property type="entry name" value="Ig_NUP210_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24902">
        <property type="entry name" value="Ig_NUP210_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="Invasin/intimin cell-adhesion fragments"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="10">
        <location>
            <begin position="479"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="10">
        <location>
            <begin position="1105"/>
            <end position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1330" mass="146048" checksum="11FD79F02950D497" modified="2014-04-16" version="1">MYKDHIAPRGPLTLCFYLKQKSTLEKKKEMASATVLSLIVLMVLFYDPIFCSSASKLNIPKVLLPLARSTKINFTLEATEGCYRWSSTRPEVASIDAVDLDERQCSQRAILQARSSQLTRLTSIIIAEDVLTGQVLRCDAIVDVISEIQIVSTTRELHLEDSPLELRIHALDSEGNTFSTLAGLVFDWSIVKDADTASFSDSYNALRVLKFSESTYTPPAYISEMEKVGKQGDIILVSGIKTGNSKIKAKIQESLYKNVPAAEVRLLILENILLNPAYDIYLLVGTSIQYRVQKIRQGKITELVMPCDQYELQLQNSILGPDGDVRIPVAKLDRATSTVTALQRGQTNIVLDHKSLRMQGASRLPNSTLYVVEPGYLGFKIHPGDRWVLETGRTYEILIEVFDKSSNKVHLSDNIRIEAVFPKAYFKILESSFNGSYHHVKALQNGQTVIDAALTSVVDQDGGVHVFSIPVRNQQDVEIYNPIVLTPRILTFPWQPKIGAYQYTIKADGGSGNFTWSSSNQDVATVTVKGVMTTVSDVGVSVVQAHDVQNPLHYGKMKVYVIEPVGMDFIPCQVEARVGQTLDLPLRIFGLLSVETGEKVTLSDCSHFDLVVEVENKGIFKFLEGRLKPGQDYCSGVRVQALATGDTVLLVSYTHGHLHLSARITIAAYQPLKAIDPVSVALVTLGSSKDMLFEGGPKPWVLEPSKFFRNLTAEDPGSVGLALFGPASRNYFQHWVRATCRAFGEQVLAVTVGNQPSLTNPFPAVEPAVVKLVCAPPSRLTLVPVYTNPQLDLSCPLLQQNKQLVPVSNYRNPVLDLAVYDQQGRKFDNFSSLSIIWESTKVSLASIEPTMPMEQHIKEDGNGQKKLHGRQTVLVHRESGVTAITVTAIGYQASHLSAARVQSLYDPLTAVSATLELLLVKDVQVSPEAITIYNHPDVRAELALREGSGYFFVNTSIGGIANIAYQESRGTAEVLPIRPGILQAMVHDLCLAFPAPAKASIHISDIFEVYVRVVDKVEIGKSVKAYVRVLDDNKKPFLAKYFHFMDLKLKAASPIVSLLPLSESSENDTATFLVQGLTIGQTSLAAVVLDKSGKRISSAPQQIEVFPPFRLIPRKVTLIIGGMMQITSEGGPQPQSHIIFSIGNEKIASVNSNGLTRGITVGNVTVTGVVQAVDAETGKLVIVSQDQVHVEVLQLKAVRIRAPITRMKTGTQMPVYVMGITSSQTPFSFGNALPGLTFHWSVTKRDILDIQTRHSEASLQLPAENNFAMNVYGRTKGRTGLKVVVKTVSPASGHLEGNVPELSDEIQIQVLTCSFYFGVECLILHSIVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9K0</accession>
    <name>W5N9K0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Lysophosphatidic acid receptor 6-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9K0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017309"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017340.1">
        <property type="protein sequence ID" value="ENSLOCP00000017309.1"/>
        <property type="gene ID" value="ENSLOCG00000014045.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RA0C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244840"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5N9K0"/>
    <dbReference type="OMA" id="HALRNDW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014045">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14982">
        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF37">
        <property type="entry name" value="COAGULATION FACTOR II (THROMBIN) RECEPTOR-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="260"/>
            <end position="280"/>
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            <begin position="56"/>
            <end position="324"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="357" mass="39047" checksum="412B18E02494FD53" modified="2014-04-16" version="1">MSASGAGVRSEALPTASPAFAAQENGTGECGAVTADFQYVLLPVAYSLVFVLGMAGNAMALLHFIRTRSTMQPSNIFLVNLCAIDLLFVLTLPFNIAYHARGNDWPFGEALCKVNGSLFFGNLYGSSLFLMLISVDRYIAVVHPLQSLKLRNPKHRILLSCAVWVVLAAVILYLTLKGPLTRPFQSTGKIACMENFSSEAWRGRISTVSILAAVIGFFLPLLVIATCYPLIACKLLAPRLVEGAAPSTSASGSGRIRKKALRMVLLVLVVFLLCFAPYHLNQLVHTLRRMGVLSGCPLIHFTYRARRVAMALCSLNSCLDPLVYCLASESFRWNWRGCARCFKLGQKPPQRQKNAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAI8</accession>
    <name>W5NAI8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Shroom family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015571">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015572">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NAI8">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017647"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017679.1">
        <property type="protein sequence ID" value="ENSLOCP00000017647.1"/>
        <property type="gene ID" value="ENSLOCG00000014331.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSTC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159479"/>
    <dbReference type="HOGENOM" id="CLU_003220_1_1_1"/>
    <dbReference type="InParanoid" id="W5NAI8"/>
    <dbReference type="OMA" id="LHCSDFD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014331">
        <property type="expression patterns" value="Expressed in heart and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Shroom family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014799">
        <property type="entry name" value="ASD2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF35">
        <property type="entry name" value="PROTEIN SHROOM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51307">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="ASD2" evidence="10">
        <location>
            <begin position="1280"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="239"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="425"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="534"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="967"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1022"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1126"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1283"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1056"/>
            <end position="1067"/>
        </location>
    </feature>
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        <location>
            <begin position="1126"/>
            <end position="1138"/>
        </location>
    </feature>
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        <location>
            <begin position="1142"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1570" mass="174131" checksum="CFE22EF1ADACDD77" modified="2014-04-16" version="1">METIEQLVSFHHIQVQLNGGAPWGFTLKGGLEHGEPLIITKIEEGGKAALCKKLRVGDELVNINGSALYGSRQEALILIKGSYRILKMVVRRRSVPVIRPHSWHLAKLSESSADAPAMHLHPGSYNLPWHTGGDASDLPMQWTQLSRNCSTDRSSSIGSMESLDQPSHGYYERQLSPVDQAVFNNKRDSAYSSFSASSNTSDYTVSLRADEASSMDNLLQGLGSCRHADGRCLHGAGEAGEFSEEGKSRFPAKHPEAKGRPSSYSYEEDRENAIAREPPQPPARRESFIATQAVSGSIDKRRASAPVDMLSLSSSWIRESRSSMRSEASNQECCPCDTGGSRDCKENGTVEQYYTLSSQLEPCCDYQETDRLDASPLLDEASRPDPIDSSSHVSFVSKKKHLVMAHRHSAPEKLVSSQLHCLDLSNSSQGRSSSPHNQWVKSSLCHGESPEAPSSDALSQSKWAESRCSTPGSAVNSEMEESRVEADVLEAALPTQHTWGRSLSVPGDTVSCHSKPVQAFDSTNQSMDVLLEEQLIPKPNSSRHRSSKSRRRSERFATNLRNEIQRKKAQLQKSKGSSSLLYGEEMVEEVSEQPECEAAGGESVPSSQGMDSRPSKASIASGAKIGLPSTGSPKKTVCATASPPGQVSWPDNGTRCQEADEVGACGKGGRWKWTPEHKLQPKGMPERKCKWEVTRAPRTTKLADAPEECDIPPFADRRKFFEETSKSLSTSHLPGLVTRHNKPTHPRTLQAKLEHSGSSLEQSWRRYSASAGFSSKEASVCCPSESRGLEQQVVNHQEKEECDPEQDFCCGHQLLKPVPLEPQPCFVNEDCKRSPKRCQQPCKHYARPQHSAFHPVTAAVCDDCSPPRVPCNPRSRTPTENLNITTAVLMLKHFSKYNVCRKHSYEGLWNIRTANPQKAHPARDQEHTNTNRKLSLTERDYQHHKHDLQTAEGAVEQENTHWGRWSEAQGFDLPSPPPHPDERAPCYPQRGRALSESDIRLDLQRLRHRAMVFGGQALSELEEAAPRKKGPPPPRPPPPNWEQYRRRRASQHSLYAPSSPCEPAAPSQLTRPVHPGHNLETSRQRSHSLPLRGFSESCLHCCKEHVATPRSPTSPPATFTPACCLREDSEHGDSEPRGATDSLAQPPNLHTSTEAEMDRPAALRLVTVSQRTVEWDRSSVPAGLHCLGINAENGSRGLPESYFSMNYEQHRELNRSREEPSKPAQKALPVHHFSETPLETDIDEFKEDEIEEELARIGHNDPQHVTVLETNIDTLPQEVGCPVAESRSGGTSTADVLGPDVGLKSRTDLTGELFPQMGGECGLGQPRRCSRSALEFSVDTLEKRSRQGSSGSSCSAYYSTSAAKAHLLSKMKELPGLNQEEEEDELFFKKQQLIDSLRKKLTVLREAQKDLQEDITANGALGEEVETLVQAVCKPNEVDKFRMFIGDLDKVVNLLLSLSSRLVRVESTLDSLSPDANHHERLTLLEKKKQLLGQLGEAQELKEHVDGRERAVCQVLARCLTPDQLRDYSHFVKMKAALLVEQRQLEDKIRLGEEQLKGLRESLGMGYGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB08</accession>
    <name>W5NB08_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adaptor related protein complex 3 subunit sigma 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB08"/>
    <dbReference type="FunCoup" id="W5NB08">
        <property type="interactions" value="1460"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017817"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017849.1">
        <property type="protein sequence ID" value="ENSLOCP00000017817.1"/>
        <property type="gene ID" value="ENSLOCG00000014474.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193421"/>
    <dbReference type="HOGENOM" id="CLU_061221_2_2_1"/>
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    <dbReference type="OMA" id="MNDQGKP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014474">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
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    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14834">
        <property type="entry name" value="AP3_sigma"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AP_complex_ssu"/>
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    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027155">
        <property type="entry name" value="APS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="6"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="197" mass="22565" checksum="44E5301205DE33B9" modified="2025-10-08" version="2">MKQEKMIKAILVFNNHGKPRLIRFYQYFPEDMQQQIIRETFHLVSKRDDNVCNFLEGGSLIGGSDYKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVHYILQEVVMGGMVLETNMNEIVAQVENQTRLEKSEGGLSAAPARAVSAVKNMNLPEIPRNINIGDINIKVPNLSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB58</accession>
    <name>W5NB58_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01023674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB58"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017867"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017899.1">
        <property type="protein sequence ID" value="ENSLOCP00000017867.1"/>
        <property type="gene ID" value="ENSLOCG00000014516.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0612">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234517"/>
    <dbReference type="InParanoid" id="W5NB58"/>
    <dbReference type="OMA" id="VCQYPLV"/>
    <dbReference type="OrthoDB" id="2156623at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014516">
        <property type="expression patterns" value="Expressed in muscle tissue and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000017">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF79">
        <property type="entry name" value="LOW QUALITY PROTEIN: MYOTONIN-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="69"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="336"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="470"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="593" mass="67315" checksum="0F835E88C2EDF006" modified="2014-04-16" version="1">MSGGSCPREGVLQSPGPVGLQTLLDLLVGVYEEFRVSPLAGEKYVAGFLQWAGPLVQQVKKTRLQRDDFEILKVIGRGNFSEVAVARMRRTQQVYAMKIMNKWDMLKRGEVACYQEEREVLVRGDRRWITELHFAFQDDNNLYLVMDYYVGGDLLTLLSKFGDRIPEEMAQFYLAEMVMAIDSVHRLGYVHRDIKPDNILLDAQGHVRLGDFGSCLRLQDNGLIHSSLAVGTPDYLSPEILLAVEDSTRGYGPECDWWSMGICAFEMFFGTTPFYADSITETYAKIIHFQEHFEFPSTDPPVSEDARALIGGLVCERSHRLGAGEFEDFRGHPFFAGIDWDTLRHCQPPFQPEVSNATDTSNFDVVDDCLSDVETLSEVLEDSPAGVHLAFVGYSYTATKETGAQDRSQDIMMEIDRQQLTEEESLLFQERLSQVWRLRAVLPAEVPELLELPLALGQRAAGPVRCQEEMDVSTNQNVDLKSRLEEAEKRNWELEGEIERLRLEIQGLRSPGHAELRICQSHHTLPAWCPDLMWDSSATPSVCQYPLVIPIHRHLLLFHRVRWPLVRTEGYLLVCAAGSDLHAWSRHCCTELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBL4</accession>
    <name>W5NBL4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Calpain 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBL4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018023"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018055.1">
        <property type="protein sequence ID" value="ENSLOCP00000018023.1"/>
        <property type="gene ID" value="ENSLOCG00000014633.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166395"/>
    <dbReference type="HOGENOM" id="CLU_010982_0_1_1"/>
    <dbReference type="InParanoid" id="W5NBL4"/>
    <dbReference type="OMA" id="QAVGMQC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014633">
        <property type="expression patterns" value="Expressed in zone of skin and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16182">
        <property type="entry name" value="EFh_PEF_Group_II_CAPN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000011">
        <property type="entry name" value="Calpain 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000114">
        <property type="entry name" value="Calpain a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF393">
        <property type="entry name" value="CALPAIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="10">
        <location>
            <begin position="35"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="612"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="375"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="709" mass="78217" checksum="6E0283CB75CF6F00" modified="2014-04-16" version="1">MSSAGPAVGSRSNPAKFKNQEFASLRAQCLRSGSLFEDPEFPAQQDSLGLPSDPDPAKAVVWRRPKEISKEPKFIEGTASTTDICQGQLGDCWLLAALSCLTLHQPLFNNVVPGDQSLSEEYAGIFHFKFWQYGQWVEVVVDDRLPVQRGRLLFSYSRTQNEFWSALLEKAYAKVNGCYASLKGGNISEAMEDFTGGIARSLPVKSRTPAVLWRAVGESLSRGTLLSCFIQAASAAEVGKVTPQGLVKGHAYSITDSSAEVCLFRLRNPWGFVEYCGPWSDKCEDWDRVDNAEKQRIKLIRAEDGEFWIKADDLSHLFTTVEMCSVNPDALGGAQSLWRITAHEGAWVPGCSAGGSRKFRRTFCKNPQFRLILSQQDREEVAEGDEDEEEEAAEDGDGVDDEGGEVEQAGQPAGRGTQGSVVLELLQKHRRQKDKISFLYIAFHVYRIPPELQDVPGSLAADFFSSNKPVARSGKYRQLRGVRKKVKLEPGHYVIVTSSYKPNVEGSFFLRIYAKTGNELGNQGGFDCSSEFATVVQAEPITAEDRGTVRGLFEELAGRDYRLNAVELMRLASSVFPENFQLSLETCRSLIFGEDTGGRGTLSLGETEQLVASLRSLQAIFSQFDEDSSGTMSPFELSLALQAAGFQLDAPVLQLLWLRHGTADLSLTFDGLVACVGKLRKLFDLYESEGSSGVKERGINAWLLRYIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBW8</accession>
    <name>W5NBW8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine peptidase inhibitor, Kunitz type, 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023835">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018127"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018159.1">
        <property type="protein sequence ID" value="ENSLOCP00000018127.1"/>
        <property type="gene ID" value="ENSLOCG00000014723.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160348"/>
    <dbReference type="InParanoid" id="W5NBW8"/>
    <dbReference type="OMA" id="CDWDQST"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014723">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000006">
        <property type="entry name" value="Serine peptidase inhibitor, Kunitz type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47247">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47247:SF1">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07502">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50986">
        <property type="entry name" value="MANSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867540" evidence="9">
        <location>
            <begin position="22"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="MANSC" evidence="11">
        <location>
            <begin position="35"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="10">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="10">
        <location>
            <begin position="236"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="10">
        <location>
            <begin position="312"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="429" mass="46140" checksum="C8DD28F09A731808" modified="2014-04-16" version="1">MAQVWYAFAALCLFLSGTGQAQDLGEKDGCPEAFVKLTGQGLDQQSFDNGASYSGHLPDVSDEASCRQACCGHKECTLALLESTAGAPAECHLVSCLSAGRDVCVLTAKDGFTAFRAAPVDRAGSNSTGKYRTRSTVANCLSPAVMGPCRAAFPRFYYSAPNQTCLPFTYGGCEGNANNYETVEQCEAACSGVKAANVQAHNAVLLMRTPGPESIRADLPEGDGKTMPSDEYAEKCEAPVKVGLCRASIPRYFYNKTTHTCHLFIYGGCHGNQNNYLSEEECLASCQVSVIPVPKKTATDSSKEYHGITDSCLASPEVGPCRGAFPAFFFSPSTQDCLPFTYGGCQGNQNRYSSAQECLAHCVGSQGYHNGHGHRFTPAFIMATTLAIMTGLLVLALILLSLRKVRRRLHSRRDDKEELLPREDSSQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCE2</accession>
    <name>W5NCE2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Bone morphogenetic protein 16</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023895">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCE2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018301"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018333.1">
        <property type="protein sequence ID" value="ENSLOCP00000018301.1"/>
        <property type="gene ID" value="ENSLOCG00000014862.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688763"/>
    <dbReference type="KEGG" id="loc:102688763"/>
    <dbReference type="CTD" id="100329171"/>
    <dbReference type="eggNOG" id="KOG3900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155666"/>
    <dbReference type="HOGENOM" id="CLU_020515_4_1_1"/>
    <dbReference type="InParanoid" id="W5NCE2"/>
    <dbReference type="OMA" id="QHIEQAN"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014862">
        <property type="expression patterns" value="Expressed in heart and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19390">
        <property type="entry name" value="TGF_beta_BMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000103">
        <property type="entry name" value="Bone morphogenetic protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000043">
        <property type="entry name" value="Bone morphogenetic protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047953">
        <property type="entry name" value="BMP2_TGF_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF246">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867551" evidence="13">
        <location>
            <begin position="21"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="280"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="398" mass="45476" checksum="32DE57453CBA4032" modified="2014-04-16" version="1">MFPANLLVLMVLLLPQVVSAREGASDPSTPPQLSLAQTIQNLLLTRLGLRNRPEPRPGTPIPQYLLDVYRFHSQDFHLIQDPGFRFPTRHTQGANTLRSFHPLETPRHLLPSSQGETDSFHIVFNVSSIPRDERVTSAELRLSRREQSVPSGTQRVNLYQLPDPRSPADTETRLLASRLLADDHDSHSWESFSLGADLFDTPNGQTHHVAFVLEVTHLNSSRPPEQRRERLRVRRSVGQDQPSWAQERPLLVTYSHDGRGQPLAQKRVEPRRTRGPWERRVKRNGRLHKLKKMARARCRRHPLYVDFKDVGWNKWIVAPSGYHAFFCMGECRFPLADHMNSSSHAMVQTLVNSVNSNVPRACCVPTTLSPIAMLYLDQHDRVVLKNYQDMVVEGCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCI3</accession>
    <name>W5NCI3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kazal-type serine peptidase inhibitor domain 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01006246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCI3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018374.1">
        <property type="protein sequence ID" value="ENSLOCP00000018342.1"/>
        <property type="gene ID" value="ENSLOCG00000014896.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063555"/>
    <dbReference type="InParanoid" id="W5NCI3"/>
    <dbReference type="OMA" id="CCMVCGQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014896">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005520">
        <property type="term" value="F:insulin-like growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011390">
        <property type="entry name" value="IGFBP_rP_mac25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186:SF25">
        <property type="entry name" value="KAZAL-TYPE SERINE PEPTIDASE INHIBITOR DOMAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045152886" evidence="6">
        <location>
            <begin position="24"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="8">
        <location>
            <begin position="51"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="121"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="171"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="31998" checksum="3BB54957305FEA07" modified="2025-10-08" version="2">MTAMSRWRSLLILGLLWTGESAGFPTGSEAGGAEAVAAGALESSGTPGRGQRARCAGCSPELCAEAVGCRAGLVPDRCGCCLECGNSEGQPCDLDRTSGFYGLCGPDLKCTLDTSDLSRGEVPEPLCACKSQEALCGSDGKTYENICKFREAELSSQALRFNSSGPCQTVPDIKRPPANLVTATGDSAIFPCEVFAFPMALSEWRRDGDDVILPGDDPRISVQSRGGPLRYETSSWLQIEGVTLGDAGTYRCIAHNGLGSVSASADLTVLGQDSGNMTDVDASLVPLSTAETFPSELPGEYDDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCJ6</accession>
    <name>W5NCJ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hypoxia-inducible factor-proline dioxygenase</fullName>
            <ecNumber evidence="9">1.14.11.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-prolyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:48400"/>
            <dbReference type="Rhea" id="RHEA-COMP:12093"/>
            <dbReference type="Rhea" id="RHEA-COMP:12094"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.29" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01023923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCJ6"/>
    <dbReference type="FunCoup" id="W5NCJ6">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018355"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018387.1">
        <property type="protein sequence ID" value="ENSLOCP00000018355.1"/>
        <property type="gene ID" value="ENSLOCG00000014905.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160655"/>
    <dbReference type="HOGENOM" id="CLU_022206_0_1_1"/>
    <dbReference type="InParanoid" id="W5NCJ6"/>
    <dbReference type="OMA" id="NKMNDNG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014905">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160082">
        <property type="term" value="F:hypoxia-inducible factor-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031545">
        <property type="term" value="F:peptidyl-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043523">
        <property type="term" value="P:regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000005">
        <property type="entry name" value="Egl nine homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF6">
        <property type="entry name" value="PROLYL HYDROXYLASE EGLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="12">
        <location>
            <begin position="144"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="272" mass="30045" checksum="C8A87C7609ED1CC1" modified="2014-04-16" version="1">RAGPRPGLLGAGAGGAGGECKRRRTEPPHAVPAARWCPERVALQYIVPCMKYYGLCVKDAFLGAELGAKVLREVETLDQSGKFRDGQLVSQRSLPPRSIRGDRIAWVEGREPGCQHIGALMAHIDEVVMYSSLNGRLGDCVINGRTKAMVACYPGNGTGYVRHVDNPNGDGRCITCIYYLNKDWDCKVHGGLLQVFPEGRSVVANIEPIFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAEAKEKYRLAAGQKGVQVPVTQSGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCW8</accession>
    <name>W5NCW8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cytoplasmic polyadenylation element binding protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation and early development. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628999.2">
        <property type="nucleotide sequence ID" value="XM_006628936.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCW8"/>
    <dbReference type="FunCoup" id="W5NCW8">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018477"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018509.1">
        <property type="protein sequence ID" value="ENSLOCP00000018477.1"/>
        <property type="gene ID" value="ENSLOCG00000015009.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696110"/>
    <dbReference type="KEGG" id="loc:102696110"/>
    <dbReference type="CTD" id="79079"/>
    <dbReference type="eggNOG" id="KOG0129">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155524"/>
    <dbReference type="HOGENOM" id="CLU_014948_1_1_1"/>
    <dbReference type="InParanoid" id="W5NCW8"/>
    <dbReference type="OMA" id="SVWPNWD"/>
    <dbReference type="OrthoDB" id="10033548at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015009">
        <property type="expression patterns" value="Expressed in ovary and 4 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="C:synapse"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
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    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12723">
        <property type="entry name" value="RRM1_CPEB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12725">
        <property type="entry name" value="RRM2_CPEB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000086">
        <property type="entry name" value="Putative Cytoplasmic polyadenylation element-binding protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative Cytoplasmic polyadenylation element-binding protein 1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032292">
        <property type="entry name" value="CEBP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
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        <property type="entry name" value="CPEB1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF9">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16368">
        <property type="entry name" value="CEBP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="310"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="429"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="565" mass="62595" checksum="2A65E0538F3508A9" modified="2014-04-16" version="1">MAFSLKEDLTRAHDHWDSEAPALSTCSNADIFRKMNTMLGNSLDFTGVCTTPITKTKRDNLSDLQDSDVSAVGSRMLFPSTTQESSRVLPDVNDLGLGLQSLSLSGWDRPWSNPDAESSAQTSSPSMLGMLNSPLGNILGKPLGLPPLDPIGTDFLDRFPGMAPRNSRLESRSLLDSRSSSPADSELSGFSSGSDHLCDLLSSLRISPPLPFLMGNMQKDPLKLDGGSRLDQDQLSLASAANVTTPTSLAKRWPGASVWPSWDLLDSPDGPFSIEREARLHRQAAAVNEATFTWSGQLPPRNYKNPLYSCKVFLGGVPWDITEAGLISTFRVFGPLSVEWPGKDGKHPRCPPKGNMPKGYVYLVFESEKSVRALLQACTQDLLNPDDLSEYFFKMSSRRMRCKDVQVIPWVISDSNFARCSSQRLDPSKTVFVGALHGMLNAEALATIMNDLFGGVIYAGIDTDKHKYPIGSGRVTFNSQRSYLKAVSAAFVEIKTSKFTKKVQIDPYLEDSMCQVCNRQPGPFFCRDQACFKYYCRSCWHWQHSMDILSNHRPLMRNQKNRDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDD1</accession>
    <name>W5NDD1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BCL2 associated athanogene 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDD1"/>
    <dbReference type="FunCoup" id="W5NDD1">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018640"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018672.1">
        <property type="protein sequence ID" value="ENSLOCP00000018640.1"/>
        <property type="gene ID" value="ENSLOCG00000015148.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158936"/>
    <dbReference type="HOGENOM" id="CLU_051025_0_0_1"/>
    <dbReference type="InParanoid" id="W5NDD1"/>
    <dbReference type="OMA" id="WNSARPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015148">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF10">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="307"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="385" mass="41895" checksum="5DF113D02CFD72E6" modified="2014-04-16" version="1">MAYDSLGNERGRANGGDPVWAMHQMQSNPKGTWPPYSNNSENNNWNAPMDSVPYPGYPSNYWYPPSHPAGPYSNTYSPGTEVNGQTPYNPQAMPAYHNGVYSGALHPSNPFYCGDQPPGTPPYPSQGCPNPATVGVGQHPSHHQRYPVPHCQGAPGYPPASYSHYGDGVHSVPQSHAYPAQPAVPSRPPAEAWSHPGGYGHPPHQQWQPGPQPPPGHYGNPFLSTHHSPWPSPEPATPQYDPKDSQYCGYSHPNQPPHPGGPKPRSPNPNPGQGGEYSAPPQLYGKTGTAQEPAPPREASPPSDNPGLSRVQQVLARVGLLEEDVDEFVGHKTDKSYRCLEELLTKELLELDSVETGGQDLVRQTRKEAVQKVQGILDRLERKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE00</accession>
    <name>W5NE00_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Basic helix-loop-helix family, member e41</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01026493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE00"/>
    <dbReference type="FunCoup" id="W5NE00">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018859"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018891.1">
        <property type="protein sequence ID" value="ENSLOCP00000018859.1"/>
        <property type="gene ID" value="ENSLOCG00000015327.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683524"/>
    <dbReference type="KEGG" id="loc:102683524"/>
    <dbReference type="CTD" id="79365"/>
    <dbReference type="eggNOG" id="KOG4304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161014"/>
    <dbReference type="HOGENOM" id="CLU_049895_0_0_1"/>
    <dbReference type="InParanoid" id="W5NE00"/>
    <dbReference type="OMA" id="MDEGIPR"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015327">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19750">
        <property type="entry name" value="bHLH-O_DEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000020">
        <property type="entry name" value="class E basic helix-loop-helix protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="44"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="13">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="383"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="422" mass="45523" checksum="0E38D65E79921504" modified="2014-04-16" version="1">MDDGIARLQDRQLLDHADFLGVEYSSLYMCKSKRGMKREESKEAYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTAVTEQQHQKIVALQSGDRSMKSSIQTDLDAFHSGFQTCAKEVLQYLNRFENWTPREQRCTQLIIHLNKVSAQLLPGAQLLSQQISASKGATSSRDPGSPRLEAQANCVPVIQRTHNGELNENDTDTDSGYGGEAEKCDGKPHGGTPRGLCSVRVKQEFRDDPAAKKVKLDAAGGAGVNPDPVSRPDVALLNSLMGLGGVPFGQQAPFCMPFYFISPSAAAAAAASYVPLLDKSNLEKYFYPAAAALTGPFPFLYPGVPAQAAGAFPGLPAALSLDKGCAPAVPSPGFDTAAETDLPSPAEDEQHLDGQSPSPGSLKSENDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE60</accession>
    <name>W5NE60_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 hydrogencarbonate(out) + Na(+)(out) = 2 hydrogencarbonate(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72215"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3 hydrogencarbonate(out) + Na(+)(out) = 3 hydrogencarbonate(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72219"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE60"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018919"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018951.1">
        <property type="protein sequence ID" value="ENSLOCP00000018919.1"/>
        <property type="gene ID" value="ENSLOCG00000015371.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157488"/>
    <dbReference type="InParanoid" id="W5NE60"/>
    <dbReference type="OMA" id="IGLTPFW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015371">
        <property type="expression patterns" value="Expressed in camera-type eye and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000002">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF20">
        <property type="entry name" value="ELECTROGENIC SODIUM BICARBONATE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="531"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="719"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="757"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="795"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="843"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="883"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="943"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="969"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="16">
        <location>
            <begin position="112"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="15">
        <location>
            <begin position="787"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1077"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1152"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1175" mass="132451" checksum="59166CE868AE3B1D" modified="2014-04-16" version="1">MDHDGLVERGPGSSRQRYEDDEDHHSIYIGVPVPRGYRRKRRRRRSTSSRENAEGERERCYERERQERSDTDEGAPGHHENGAAADLSRTMSPAAERLRYILGEEDDVPTPTLFTEMDTLQHDGEELEWKESARWVKFEEKVEEGGERWSKPHVSTLSLHSLFELRTCLQTGTILLDLDGYSLPQIVDDIIERQVEEGLINPELRDKISFVLLRKHRHQTKKPIHRSLADIGKSVSTSSTNRSPSRSPSAGAGFHRSTEDLCMRQARNYGRLRHAQSRSMNDISDTPSTDQLKNKFMKKIPRDAEASNVLVGEVDFLDKPFVAFVRLAQATTLGGLTEVPVPTRFLFVLLGPHGKAKSYNEIGRAIATLMVDDLFSDVAYKARDREDLIAGIDEFLDEVIVLPPGEWDPTIRIEPPKKLPSAEKRKSVFSVNEAGQMNGMAGGGGGVSEDEEMPAPHEVGEELLWTGRFCGGLFLDIKRKLPWFPSDLYEGFHIQSISTILFIYLGCITNAITFGGLLGDATDNYQGVMESFLGTAMAGTVFCFFGGQPLIILSSTGPILIFEKLLFDFSKGNDIDYLEFRLWIGLHSCLQCFVLVATDASFIIKYITRFTEEGFSTLISFIFIYDAIKKMIGAFKYYPINSDFKSDYVTLYKCECMAPDPVATMTFNVSALVAPNNTNISVYNPVNVTALDWSQLSKKECLKYGGSLVGKTCKYVPDLALMSFILFFGTYTMTVTLKKFKSSRYFPTKLRSLISDFSIVLSILVFCMVDYWLALDTPKLHVPTEIKLRKLISDFSIFVSIMMFVGIDMLAGLNTPKLIVPTEFKPTRPGRGWIVLPFGKNPWWVYLASFLPALLVTILIFMDQQISAVIVNRKEHKLKKGGGYHLDLFWVGILMAVCSFMGLPWYVAATVISIAHIDSLKMETESSAPGEQPQFLGVREQRLTGIMVFVLTGVSIFMAPILQYIPMPVLYGVFLYMGVASLNGIQFWDRCKLYLMPAKHQPDYIYLRHVPLRRVHLFTVVQVICLAVLWVLKSTVAAIIFPVMILALIVVRKMLDLVFSQHDLAWLDDILPEKDKKKEEDKKKKKKSKGGGGDNSDDEEKGHPHSNCSPCDSDLDRSITLHLKISCPSSPAVSLSRNAAFAVPQVKIEMESDYDDTDTDRHHRHHRDMGSETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEA7</accession>
    <name>W5NEA7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein 44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01039116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEA7"/>
    <dbReference type="FunCoup" id="W5NEA7">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018966"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018998.1">
        <property type="protein sequence ID" value="ENSLOCP00000018966.1"/>
        <property type="gene ID" value="ENSLOCG00000015409.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157557"/>
    <dbReference type="HOGENOM" id="CLU_009835_2_1_1"/>
    <dbReference type="InParanoid" id="W5NEA7"/>
    <dbReference type="OMA" id="ENQCIYV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015409">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="516"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="613"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="653"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="566"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="923" mass="102108" checksum="7FE9B8E51CAE9BE2" modified="2014-04-16" version="1">MASDTSDTEEFYDAAEDVNLIPSPSVSPAKFVLPPAKSNCLGGEVRRARNLSQINQEEKPDDPIEIIDSIIEESQKDSTTGDSLLVAVKQDFGDESPGPSDLGNAESVKEQLPHEGDKEPVDRSQGQDSQDGLSNDATQTPCPVEDHPKTERMSEAMKPPDITSAIAKEKPCTLVPDNRADGVPEPRKADILEQFTMADDLPDKDSSAPAKPPRQFTVEPDIVASTKKLPPARPRPPSGAPPPRPPPPTRPAPPPRKKKSDLGPESLRPSGLEVFLPFLFTVLGDTVESSGLVSPNTAVESITKELQHSLDLASATSGDKVVTAQENGKAADGQMSTSAGSETPGPQRPRSNSGRELTDEEILASVMIKNLDTGEEIPLSLAEEKLPTGINPLTLHIMRRTKEYVTNDAAQSDDEDKSQSHLTDNDGGKLKQKTTQLKKFLGKSVKRAKHLAEEYGERAVNKVKSVRDEVFHTDQDDPSSSDDEGMPYTRPVKFKAAHGFKGPFDFDQIKVVQDLSGEHMGAVWTMKFSHCGRLLATAGQDNVVRIWVLKNAFDYFNNMRIKYNTEGRVSPSPSQESLSSSKSDTETGVNSTPEDPDLEDKNVPFRQLPFCKYKGHTADLLDLSWSKNYFLLSSSMDKTVRLWHISRRECLCCFQHIDFVTAIAFHPRDDRYFLSGSLDGKLRLWNIPDKKVALWNEVDGQTRLITAANFCQNGKYAVIGTYDGRCIFYDTEHLKYHTQIHVRSTRGRNRVGRKITGIEPLPGENKILVTSNDSRIRLYDLRDLSLSMKYKGYVNSSSQIKASFSHDYSFIISGSEDKYVYIWSTYHDLSKFTSVRRDRNDFWEGIKAHNAVVTSAIFAPNPSLIVSPDVATERPEGECKNAETAENESIPSGALKTDHTEVLLSADFTGAIKVFVNRKTGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEF6</accession>
    <name>W5NEF6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEF6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019015"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019047.1">
        <property type="protein sequence ID" value="ENSLOCP00000019015.1"/>
        <property type="gene ID" value="ENSLOCG00000015449.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWCI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="W5NEF6"/>
    <dbReference type="OMA" id="TPFRMEE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015449">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="LEM_LAP2_LEMD1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Thymopoietin isoform alpha"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LEM/LEM-like_dom_sf"/>
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        <property type="entry name" value="LEM_dom"/>
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    <dbReference type="InterPro" id="IPR051656">
        <property type="entry name" value="LEM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12019">
        <property type="entry name" value="LAMINA-ASSOCIATED POLYPEPTIDE THYMOPOIETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12019:SF21">
        <property type="entry name" value="THYMOPOIETIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03020">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00540">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63451">
        <property type="entry name" value="LEM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50954">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="LEM" evidence="5">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="40959" checksum="C1102F7B4FA94717" modified="2014-04-16" version="1">GLTARGQLRAIIVKKATKKTDKPRPEDVDVADLTDEDLKDQLLKYGVSVGPIVASTRKLYEKKLQKLLDQGPPETLPPPSAPLRAADANQNGNTDSEKYSDNEEDEKLEPIVEKLEPVKTRVKTTPGLRTRRYEHNRSFNPTGKRGTEFTGGSAKPLFPLLPHSERPSRRLPAGRPEAERNFAVEAPLVDDSSLLTAPVPGCGHTWMSVPRRMRDIAHRAMSSPVSLSLPLLSHSRLPPLSPQVEERLEAGDVVRRKEAKDLLEEMFPYEPRTPTGITATCRRPIHGAAGRPLNPSDFRLDDSILRRTEFSAAKLTAPSGGQSRSRRSLPVWLQLLLLMAVAGFLFFVYQAMETNEINPFSTMVSPASPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEJ5</accession>
    <name>W5NEJ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TATA box binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638469.1">
        <property type="nucleotide sequence ID" value="XM_006638406.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217970.1">
        <property type="nucleotide sequence ID" value="XM_015362484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEJ5"/>
    <dbReference type="FunCoup" id="W5NEJ5">
        <property type="interactions" value="1703"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019054"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019086.1">
        <property type="protein sequence ID" value="ENSLOCP00000019054.1"/>
        <property type="gene ID" value="ENSLOCG00000015481.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684751"/>
    <dbReference type="KEGG" id="loc:102684751"/>
    <dbReference type="CTD" id="6908"/>
    <dbReference type="eggNOG" id="KOG3302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157474"/>
    <dbReference type="HOGENOM" id="CLU_060161_1_1_1"/>
    <dbReference type="InParanoid" id="W5NEJ5"/>
    <dbReference type="OMA" id="NMDQNNS"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015481">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="313" mass="34723" checksum="9F85CDCF1CA7FF6C" modified="2014-04-16" version="1">MEQNNSLPPYAQGLASPQGAMTPGMPLFSPMMPYGTGLTPQPVQSTNSLSLLEEQQRQQQQQQQQQQQQQQQQQQQQSTSQQGVVQASSGQTPQLYHSQTVTTTALPGNTPMYTTPLTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFI7</accession>
    <name>W5NFI7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">FYVE, RhoGEF and PH domain containing 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NFI7">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019396"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019428.1">
        <property type="protein sequence ID" value="ENSLOCP00000019396.1"/>
        <property type="gene ID" value="ENSLOCG00000015755.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685330"/>
    <dbReference type="KEGG" id="loc:102685330"/>
    <dbReference type="CTD" id="55785"/>
    <dbReference type="eggNOG" id="KOG1729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156334"/>
    <dbReference type="HOGENOM" id="CLU_004959_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFI7"/>
    <dbReference type="OMA" id="THYSSRI"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015755">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15743">
        <property type="entry name" value="FYVE_FGD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13237">
        <property type="entry name" value="PH2_FGD5_FGD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000158">
        <property type="entry name" value="FYVE, RhoGEF and PH domain containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000024">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF12">
        <property type="entry name" value="FYVE, RHOGEF AND PH DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="12">
        <location>
            <begin position="831"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="1049"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="14">
        <location>
            <begin position="1182"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1202"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="1292"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="229"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="421"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="506"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="788"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="807"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1387" mass="154317" checksum="0B841D0F6623F95E" modified="2014-04-16" version="1">MTEEKVVGIMSTDIKKPPVAPKPKIVESQKAFPPPVAPKPDIVLSHTAPALKKAKPAVAPKPLFSKSACVPETKPVILKNASRIFPDEKTKLSEDLGLLNSKNGLQTELNKLNSNYIITTCTCTVDCRSKCKHTENCKTDFVIDQLEKLEALQNGRLDGVQVSPAPRARTKHDTSHEKIGSKSQVVLNASLLEHKLKNVLVQAVSPNNHHLKFKSQERATAQKCKAGNLDLDQTSNAGGSAAELGEKCSNSEESQRETGGLDSHSTITLSNEPQSQNIPGPMESFVSSTKGTCKPNNESSVSHNTFISSPAPKKPLPIPMPRKSKKSGLVRQDCVESNVHSVDGHIEMLNMEPEGVCLKSSPFKSPKVCIHTQSVSYSRNLSESHENAGSSQGCDTGENLEIPLPVSGSFLAGELSKTPHFCGDVSGETEMGEQSKSTAGLESENGEKSAKGVVSSVEKTSVAEVDFKSEKSPKVAPKKPQRHSLSASGLLKKELLEELTVRSPCAEDVESGVPDGDCPQTEELGPSAESDRKRELPAEKPVWKLPRPIVPLFGKPEALKTGRQSVMHSKVPTLCLGKPRAKSFSSADLERTEGLSKDGQKRSSFRKLLELKLSVKKLPRFLAKSSQSLDCTSVTTDPPAYGEWSSSKENSPVLPKHGFVTNGQCETPISVNSKLDLADEPKASCSKSPEEGSLYCTSFPKQDCAVEYENLPLYEEIPEYMNLPFLMASGLRNSESPAFGWQNSSSVEDEEADLYEVQEPYLPYQKYRTHMRTNTYERPSLDQDLGLENEHSEEEEVVNSSDEDDNSSSSSKGDLNLSGDRQHDGGVKQTKVVHIAKEIMSSEKVFVDVLKLLHIDFRDAVAKASRQTGKAVIEERILNQILYYLPQLYELNRDLLRELEERVAHWNDHQRIADIFVKKGPYLKMYSTYIREFNKNVALLDEQCKKNLGFASVVKEFEMSPRCASLALKHYLLKPVQRIPQYQLLLTDYLKNLSEDSFDYKDTQAALGIVIEVANHANDIMKQGDNFQKLMQIQYSLNGHHEIVQPGRVFLKEGTLMKLSRKVMQPRTFFLFNDTLLYTTPVQSGMYKLNSMLSLAGMKVSKPTQEAYQNELNIESVERSFILSASSATERDEWLEAISKAIDDYTRKKISFFNSKSLEETDSAQVDSGAPLGSKAPIWIPDLRATMCMICTCEFTLTWRRHHCRACGKVVCQSCSLNKHRLEYLKNQPARVCDQCFSKLQQHNNQALPTGFSPTCKTSPGAFSSVLISIPSGRKQKKIPAALKEVTANTENSSISGYLYRSKGHKKQWKRLWFVIKNKVLYTYAASEDVAALESQPLLGFHLREEQSGHKLRFKLYHKNTLFYIFKADDFQTAQKWIEAFQAATIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHF2</accession>
    <name>W5NHF2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubby-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Tubby superfamily protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tubby-like protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NHF2">
        <property type="interactions" value="761"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020061"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020094.1">
        <property type="protein sequence ID" value="ENSLOCP00000020061.1"/>
        <property type="gene ID" value="ENSLOCG00000016263.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155913"/>
    <dbReference type="HOGENOM" id="CLU_002083_1_0_1"/>
    <dbReference type="InParanoid" id="W5NHF2"/>
    <dbReference type="OMA" id="DARVKCM"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016263">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000262">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000002">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF2">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="16">
        <location>
            <begin position="373"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="785"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="977"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1386"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="806"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1394"/>
            <end position="1412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1608" mass="175466" checksum="824D6E901DD9E31C" modified="2014-04-16" version="1">MFAAVEHGPVLCSDSNVLCLSWKGRVPKSEKEKPVCRRRYYEEGWLATGNGRGVVGVTFTSSHCRRDRSTPQRINFNLRGHNSEVVLVRWNEPFQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHVLLHESEGIVSMSWNYPSFLVEDSSESDTDSDDYAPPQDGPTVYPIPVQNIKPLLTVSFTSGDISLMNNYDDLSPAVIHSGLKDVVVQWCSQGDLLAVAGVERHGMPADPGCMPSMRNALVKFYNVSGEHIYTLETPAQRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLMCQQAVASALREEKDISKLTMPSRLCSYLTTAFIPTIKPPIPDPNNMRHFVSYPTPGNERLHCTMKRTEDNPEVGGPCYTLYLEYLGGLVPILKGRRISKLRPEFVIMDPKTDGKAEYDDEVYGNSLISSVIDSCNCSDSSDIELSDDWVGKKSPKISRGSKSPKLPRINIEARKSPKLSRAAQEISRSPRLPIRKPSVGSPSLARREFPLEDITQHNYLAQVTSNIWGTKFKIVGLTSFLPTSLGSVVYKTSLLHLQPRQMTVFLPEVVKVSMDYINLPVFNPNVFSEDEDDLPVTGPSGVPDDNPPCTVNIPIAPIHSSAQAMSPTQSIGLVQSLLANQNVQLDVLTNPTAAAVNVAAAAAVAGVSDHGQESMMTSQYPVSSRYTNPGQVIFGGLEMGRLLVGPPPSHHQQQQPPQMQMPQLSEHGSEISRDHEHLQKGKPLRSIPQLADGDAVVFSAPLEIQISKMNPPPPYPGTVAAAAAAVAAAAAAAAASAANSNNPAAPPVDLCLKKNEFSLYPPAPQYQTPLGYERITTFDSSGNVEEVCRPRTRIVCNQNMYALQGPGSSATLRVTSSESKKVQLPCASATLNRLTVPRYSIPSGDPPPYPDPANQTSLGRNPGQRIDSSLIHATLRRNSREAVLKVSQMMDSHRTLPTKSKVNSALAASYQQRVSTALYTCSQCSNNSNSTAGTTGAGGSGSSNSNGITGGSIVRQDFAAGSAAQHSTVIVHSNSTSPLASQSSYNLLSPPDNCRDRTEYINSAFTEDEALNQQCQLEKSLRHLTLGEVGLAVKRPPPYQWDPAATEEIWVPQERTPLGAPPGTHKPPPLILNPAQHLDVSRLPFVLSSKSPTSPNATFQAGYQIALPFPPGAGYSGPQLQAMPPPPQPCSPKEVVAPVPFPQQEPAMVLPPGYPANLANLACCPLPPMYPGTSSCAGLQLPPIGLHPWNTYSPCPPMQNPSGTLPTKAHLVVEKPVLSPPPPPADLQNHMTSPEAMDEAGETFQEVNESPIPQRADKFSKKSRKRLDSRAEEANVPAITEGKVKKEGRALGDFNSLISSPRLGREKKKPKSQKEQLKAKKLNKTNEFQDSSESEPELFISGDELMNQSQSSKKGWKSKRNLRTASELDEFKCRKANEKEDRGLGSEDFVYIMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI80</accession>
    <name>W5NI80_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-ethylmaleimide-sensitive factor attachment protein, beta b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009040">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009041">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI80"/>
    <dbReference type="FunCoup" id="W5NI80">
        <property type="interactions" value="1430"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020339"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020373.1">
        <property type="protein sequence ID" value="ENSLOCP00000020339.1"/>
        <property type="gene ID" value="ENSLOCG00000016463.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005826"/>
    <dbReference type="HOGENOM" id="CLU_046329_0_1_1"/>
    <dbReference type="InParanoid" id="W5NI80"/>
    <dbReference type="OMA" id="EKAGNMY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016463">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070044">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010807">
        <property type="term" value="P:regulation of synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000028">
        <property type="entry name" value="beta-soluble NSF attachment protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF12">
        <property type="entry name" value="BETA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="132"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="299" mass="33530" checksum="90F57FCDC318AF70" modified="2014-04-16" version="1">MDNTGKEKEAIQLMAEADKKVKSSHSFLGGMFGGNSKIEEACDMYARAANMFKMAKNWNGAGNAFCQAAKLHMQLQSKHDAATSFVDAGNAYKKADPQMHPKEAISCLNAAIDIYTDMGRFTIAAKHHITIAEVYESELVDIEKAIAHYEQAADYYKGEESNSSANKCLLKVASYSAQLEQYQKAVEIYEQVGTNTMDNPLLKYSAKEYFFKAALCHFIVDELNAKLAVEKYEEMFPAFSDSRECKLLKKLLEAHEEQNSEAFTEAVKEFDSVSRLDQWLTTMLLRIKKTIQGDAGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIM0</accession>
    <name>W5NIM0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CECR2 histone acetyl-lysine reader</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIM0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020479"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020514.1">
        <property type="protein sequence ID" value="ENSLOCP00000020479.1"/>
        <property type="gene ID" value="ENSLOCG00000016580.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166757"/>
    <dbReference type="HOGENOM" id="CLU_029092_0_0_1"/>
    <dbReference type="InParanoid" id="W5NIM0"/>
    <dbReference type="OMA" id="CATENDW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016580">
        <property type="expression patterns" value="Expressed in ovary and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090537">
        <property type="term" value="C:CERF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029614">
        <property type="entry name" value="CECR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47092">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47092:SF1">
        <property type="entry name" value="CHROMATIN REMODELING REGULATOR CECR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="42787" checksum="EA33EDE0BDA7440E" modified="2014-04-16" version="1">GTVHSRTWLWCACLCGKRHRFNSVFPLPFSQELEEALLKQDFAFLSELISSLLQGCYQRTDITPQAFRSYLEDIINYRWELEEGKPNPLKEGPFEELPTRTQVELLHRLCDYRLDAADVFDLLKGLDADSLRVEPLGEDSDGALYWYFYGTRMYKEQPVRRTTAAQSCACFSFVNSENAEVKVEEKPVRKRGRPPKKKKFEAKRLSWLYSPSKASKDTAKEQSRYSDRKSERGAWSLVCATEEEWQSLAESIKGKHTYKDRQLYKLLSENFIPEISNMILYKENQRQERLLDMAPRRSSDRLTIKRILQEEEETLQAIAEVEQQKRKEEEEVDRQLLLAEHRREQERQLEEERRQEVQDKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJX4</accession>
    <name>W5NJX4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SVOP-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023533">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023534">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJX4"/>
    <dbReference type="FunCoup" id="W5NJX4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020933"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020969.1">
        <property type="protein sequence ID" value="ENSLOCP00000020933.1"/>
        <property type="gene ID" value="ENSLOCG00000016932.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158632"/>
    <dbReference type="HOGENOM" id="CLU_001265_46_0_1"/>
    <dbReference type="InParanoid" id="W5NJX4"/>
    <dbReference type="OMA" id="PTWIGVC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016932">
        <property type="expression patterns" value="Expressed in ovary and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF45">
        <property type="entry name" value="SVOP LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="57"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="495" mass="55301" checksum="077C7BB444BBC0AF" modified="2014-04-16" version="1">TFTTMSAGIRKTLVSSIQLQDINHRADEIPQEVKEEKTYTIEQAVEEIGFGRFHVLLFLIMGSANIVEAIEIMLLAVVSPEIRCEWRLEDWQVALVSTMVFFGFMTCGVLSGYVADRYGRWKVVFGAFIWAAYFSLLTSFSPSYGWFIFLRTMVGCGVAGTSQGFVLKTEFIPAKYRGYLLPLSQIFWMLGAVLMVFLAMTVIPSLGWRWMIRISIIPSIILMFLFQFIPESARYNVSAGNVSGALQTLQRIARMNGSSLPEGKLVEPLVTQRGSFYILVSSTFRRTSMLLWYSWFVASFSYYGSVLSSSELLEKNLLCITNAESEHKINPEVTPCYCIPFGNSDYHTMLISSLGEISLLPLNIIMLNTLGRKYSMAILMICSGIFFLLLSICSTLFGFTILLFLLRSIVAMNFNAVYIYTAEVYPTSVRSIGLGFCTSFSRIGGMIAPFIAQVLMSKSVITALVPFAVTSFLCSVGALLLPIETKGRALLQNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK08</accession>
    <name>W5NK08_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 UFM1-protein ligase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">E3 UFM1-protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK08"/>
    <dbReference type="FunCoup" id="W5NK08">
        <property type="interactions" value="1743"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020967"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021003.1">
        <property type="protein sequence ID" value="ENSLOCP00000020967.1"/>
        <property type="gene ID" value="ENSLOCG00000016960.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002112"/>
    <dbReference type="HOGENOM" id="CLU_012417_1_1_1"/>
    <dbReference type="InParanoid" id="W5NK08"/>
    <dbReference type="OMA" id="CILHASG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016960">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071568">
        <property type="term" value="F:UFM1 transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056761">
        <property type="entry name" value="Ufl1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056580">
        <property type="entry name" value="Ufl1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23659">
        <property type="entry name" value="UFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25041">
        <property type="entry name" value="UFL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25870">
        <property type="entry name" value="WHD_UFL1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like" evidence="8">
        <location>
            <begin position="541"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase-like C-terminal" evidence="9">
        <location>
            <begin position="664"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="795" mass="89485" checksum="3876E22FA2D9240A" modified="2014-04-16" version="1">MAADWEEIRRLAADFQRAQFADTIQRLSERNCIEIVAKLVEDKQLDVVHTLDGKEYITPSQISREIQDELYVHEEGRVNIVDLHQIINVDLSHIESRASEIAKSVKGVQLVLGQLIDENYLDRLAEEVNDKLQEAGQVTIAELCKTYDLPGDFLTEVLSERLGRIIQGQMDQYDRGVIFTQAFVCRHKARICGLFSAITRPVPVNNLISLYGFQEHLLYSVLEELVSCGRLKGTVVGGRQDKAVYIPDIYTKTQNNWIDSFLKQNGYLEFDALTRLGIPDPLNYIKKRYKSEKLLFLRAACVGQNIVDQVEASVEEAVSSGTWIDVQPMLPSCLSLDDTGILVNQVMRTSNVQSSARILNGSIVVSEKFISSCISLFDQSMRMKAEKEMKNNPVFLITEEDIKQASVLAENSAPSKKDKRDERRKKAGEGSGSVKGGGGGNSREIRIRKTKKKGRREDDSDEDTDAATQNRNKQQEVKFMSQEEIEGVLQKHICDCPEDMISELAQHLIRPLAKMYQDVVHTVFMSSTSSTTGVSRKQSVKDLQEEINILYNNIRLFEKGTKLFSDDTQANIAKHVLKTMCTDVTNLLFNFVAAELMMALENYSSITYEVRVKILGKLPEETKGPLMKLHNSLNGKSIEDFLTNLEAVSEVCGIMLKKGDKKKERQALFQHRQALTEQLKVTEDPALVLHLTSVLLFQFTTHCMLHAPGRCVPQIIAALSAKISQDQHKLLTRYQGLVVKQLVGQNKKPNKAEREAAEGQSEEDLTEAVGKELLSLTSEVKDLVLRSRKSSLTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL01</accession>
    <name>W5NL01_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 13-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01032650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL01"/>
    <dbReference type="FunCoup" id="W5NL01">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021310"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021347.1">
        <property type="protein sequence ID" value="ENSLOCP00000021310.1"/>
        <property type="gene ID" value="ENSLOCG00000017229.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154607"/>
    <dbReference type="InParanoid" id="W5NL01"/>
    <dbReference type="OMA" id="WRLSLIF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017229">
        <property type="expression patterns" value="Expressed in mesonephros and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="23"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="527" mass="58891" checksum="4ED6630F70BB6C90" modified="2014-04-16" version="1">SEERRICVFRVMGPLQKSVYWRLSLIFVFTAFMFFVDVFTVHHFSCASLRESGNVNFLDESPEKNFSYDISNENRSLSIAFAESFNVSTNKRGTENKNKHVMSSECGDNKLREYGLTMYMSGLLLGSLFGGALSDKYGKKVLLVGCSTIQAIIALMPAFIPNAIFHLTARLIGGIMCCGINIASFSLGVEWSLPRYHVWPPALLSFSFSIGMMFLALIAYLASNWQQFYLAIAVPQLLCIPLYFCIPESPRWLLMKKRFSTLEEYRHESRKDQENLDLILETVGMKTQQPIEQNNASERISDFQHFKSQTIVQRLCIMSFISLSSALTYYGICFNVGNFGVNIYLAQFFSGLTEMPTLLVPLLLVRFGRRTFTMISLLMSGSGCLLSLLVSKLCDMPSLVMALALIGKLCMQTTVFVSLLYGIELFPTVIRQKCVGFVNLWYRIGCILNAVLSPKDGISLEAMICYGSGPILGAGLCLLLPETSGISLPDTVQDCKKQPVPSLFSKSRKVHVPGKDCSGLEPIQELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMF3</accession>
    <name>W5NMF3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the iodothyronine deiodinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMF3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021812"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021849.1">
        <property type="protein sequence ID" value="ENSLOCP00000021812.1"/>
        <property type="gene ID" value="ENSLOCG00000017707.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5FA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154482"/>
    <dbReference type="HOGENOM" id="CLU_103502_1_0_1"/>
    <dbReference type="InParanoid" id="W5NMF3"/>
    <dbReference type="OMA" id="NTANAEY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017707">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000324">
        <property type="entry name" value="Iodothyronine deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF23">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="1" id="KW-0893">Thyroid hormones biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="117" mass="13301" checksum="BC2479C8DC8123AA" modified="2014-04-16" version="1">VVSQYADIADSLLVYIEEAHPSDGWVSSDAPYQIPKHRCLEDRLKAAKLMNLEVPRCLVVADTMENSSNAAYGAYFERLYILQDGKVVYQGGRGPEGYRISELRNWLDQYKNNLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMN8</accession>
    <name>W5NMN8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retinoic acid induced 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020275">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219450.1">
        <property type="nucleotide sequence ID" value="XM_015363964.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMN8"/>
    <dbReference type="FunCoup" id="W5NMN8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021897"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021935.1">
        <property type="protein sequence ID" value="ENSLOCP00000021897.1"/>
        <property type="gene ID" value="ENSLOCG00000017793.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689427"/>
    <dbReference type="KEGG" id="loc:102689427"/>
    <dbReference type="CTD" id="10742"/>
    <dbReference type="eggNOG" id="ENOG502RCMZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154164"/>
    <dbReference type="HOGENOM" id="CLU_044853_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMN8"/>
    <dbReference type="OMA" id="QHKWLVD"/>
    <dbReference type="OrthoDB" id="9936033at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017793">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026092">
        <property type="entry name" value="RAI2/SOBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23186">
        <property type="entry name" value="RETINOIC ACID-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23186:SF3">
        <property type="entry name" value="RETINOIC ACID-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15279">
        <property type="entry name" value="SOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="574" mass="61351" checksum="5A41D1A5425429AF" modified="2014-04-16" version="1">MEDLYKDAQPNLPLDIGSPQSTVGSSGAAGSGGSGGKLENGMTQLITAEAWNINPTGLMKKALSPLVAVPAPPIVTPPTESPGGVALKVAATVLQPICLGDSPVVLPIHLQVAGSATPQVTPAGNTPYVMTNQGPVPLPLVLEQQVFQHLNSPVLSQGAPCPAISLQNNMLCQNSSLSFNQPQPIDQKPSGQPQEPGILPLLQNPGFAAILQDLFPTQNNLNSSPCHSAASAPTDPFSSNFFPPPPPPSQAYSFPLSPLVPPATLLVPYPVIVPLPVPLPIPIPVPIPIPQNSESKGFTGPPKQACTLSKSTQTATKESLSPLTLQNRDFSSNHSQLASPSLQPGDVEVLDLSMKSMPLQTKQEIHFQQDSVLDLSVTSIRKQCFQTALLDPSRDLPRDGLEHPSHPREDTTSTALSLEVSHPVEGTQKLDSKMLNSLASLEFSRQHKWVMDPGTTRPGCEPKCSSGNIEIVSTSQTAKVIVSVKDAVPAIFCGKIKGLSGVSTKNFSFKRDSTQESMLQQCYDAKNQAEQRDANDPLKKSLKNRSIKLKKMNSQEIHILPIKKQRLAAFFPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN74</accession>
    <name>W5NN74_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006062">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN74"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022083"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022124.1">
        <property type="protein sequence ID" value="ENSLOCP00000022083.1"/>
        <property type="gene ID" value="ENSLOCG00000017982.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QZSZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165893"/>
    <dbReference type="HOGENOM" id="CLU_065229_0_0_1"/>
    <dbReference type="InParanoid" id="W5NN74"/>
    <dbReference type="OMA" id="NIMEYNA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272">
        <property type="entry name" value="DDE_TNP_1_7 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272:SF1">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="1">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="209" mass="23777" checksum="CD52350A256A41F5" modified="2014-04-16" version="1">LLKRGRGSFDYQVDNDAQLAIVKWADSKTVTLVSTCASVMPVGEVKRFSKKKGRQISVPCPQIVAEYNHHMGGVDLAYMMITLYRTPAKSHRWYLCIFWQLIDIAVNNAWLFYRRDATAIGPVKLKKLKAFRLDVAKGLIYPEKQGWPSLGNENDAPVRVVKQPKVSRPIDEMRYDGNSHYPILSVKGRCRMSPDGQTSIMCEKCNVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-03-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q8HQM7</accession>
    <name>Q8HQM7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytochrome c oxidase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">COIII</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="16 17"/>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2003" name="Mol. Phylogenet. Evol." volume="26" first="110" last="120">
            <title>Basal actinopterygian relationships: a mitogenomic perspective on the phylogeny of the 'ancient fish.'.</title>
            <authorList>
                <person name="Inoue J."/>
                <person name="Miya M."/>
                <person name="Tsukamoto K."/>
                <person name="Nishida M."/>
            </authorList>
            <dbReference type="PubMed" id="12470943"/>
            <dbReference type="DOI" id="10.1016/S1055-7903(02)00331-7"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2003-10" db="EMBL/GenBank/DDBJ databases">
            <title>Molecular Evidence for Permian teleosts.</title>
            <authorList>
                <person name="Dunn K.A."/>
                <person name="Yang Z."/>
                <person name="Coates M."/>
                <person name="Thomas M.G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AY442350">
        <property type="protein sequence ID" value="AAR16135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB042861">
        <property type="protein sequence ID" value="BAB40735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8HQM7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022477"/>
    <dbReference type="Ensembl" id="ENSLOCT00000025130.1">
        <property type="protein sequence ID" value="ENSLOCP00000022477.1"/>
        <property type="gene ID" value="ENSLOCG00000020988.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:807204"/>
    <dbReference type="eggNOG" id="KOG4664">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013064"/>
    <dbReference type="HOGENOM" id="CLU_044071_0_0_1"/>
    <dbReference type="OMA" id="SIYWWGS"/>
    <dbReference type="OrthoDB" id="10050457at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000020988">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01665">
        <property type="entry name" value="Cyt_c_Oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000048">
        <property type="entry name" value="Cytochrome c oxidase subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.80:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="entry name" value="Cytochrome c oxidase, subunit III, four-helix bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033945">
        <property type="entry name" value="Cyt_c_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403:SF7">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="Cytochrome c oxidase subunit III-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Heme-copper oxidase subunit III family profile" evidence="14">
        <location>
            <begin position="4"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAR16135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAB40735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="261" mass="29713" checksum="DF3338D1A1EB2745" modified="2003-03-01" version="1">MAHQTHAYHMVDPSPWPLTGAIAALLMTSGLAIWFHFGSTSLMTLGSMLLPLTMYQWWRDIVREGTFQGHHTPPVQKGLRYGMILFITSEVFFFLGFFWAFYHSSLAPTPELGGCWPPTGITPLDPFEVPLLNTAVLLASGVTVTWAHHSLMEGARKQMIQALTLTIILGFYFTMLQAMEYYEAPFTIADGVYGSTFFVATGFHGLHVIIGSLFLLVCLLRQIQYHFTPEHHFGFEAAAWYWHFVDVVWLFLYVSIYWWGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW02</accession>
    <name>W5LW02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LW02"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000309"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000309.1">
        <property type="protein sequence ID" value="ENSLOCP00000000309.1"/>
        <property type="gene ID" value="ENSLOCG00000000283.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ5W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155987"/>
    <dbReference type="HOGENOM" id="CLU_1247860_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW02"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000283">
        <property type="expression patterns" value="Expressed in bone element and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000012">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000058">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053243">
        <property type="entry name" value="SJ_maturation_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47653:SF1">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47653">
        <property type="entry name" value="PROTEIN BARK BEETLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="45"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="222" mass="24240" checksum="D65A2EED6E90438D" modified="2014-04-16" version="1">MMASFSQGTGPIWLDEVGCLGNESSLWQCPSVGWGQHDCGHKEDVRILCSDHTLLRLSAGCSGQAEVYYNGTWGSICANGMTDTTAAVICKQLGCGDKGTIRETNSRLRPDPKWLDFISCRKHDSTLWQCPSSPWGQNDCTDREVANITCSGPRKEADRSTPAPQEEQPQLYCPVPLALRLAGGTNCSGRVELRYEGSWGTVCDDSWDLEDAQVVCRQLGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWD5</accession>
    <name>W5LWD5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWD5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000442.1">
        <property type="protein sequence ID" value="ENSLOCP00000000442.1"/>
        <property type="gene ID" value="ENSLOCG00000000406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LWD5"/>
    <dbReference type="OMA" id="CKASSHV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000406">
        <property type="expression patterns" value="Expressed in mesonephros"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:004829">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="112" mass="12105" checksum="980F9EBE75F3836D" modified="2014-04-16" version="1">RFLYILSHNVLFGFSFSESRGQTTVTQIPAVKSVQPGQTVTVSCKTSTAVLSNNFHHWYQQKPGEAPKPLIYLTGIPEHFSGSGSGTGVQAVDAADYYCQGVHIISTNVLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWI1</accession>
    <name>W5LWI1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWI1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000488"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000489.1">
        <property type="protein sequence ID" value="ENSLOCP00000000488.1"/>
        <property type="gene ID" value="ENSLOCG00000000447.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5TB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_5_2_1"/>
    <dbReference type="InParanoid" id="W5LWI1"/>
    <dbReference type="OMA" id="EEIFICK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000447">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003552">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865216" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="9"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="119" mass="12903" checksum="3851BC0F5E040AF4" modified="2014-04-16" version="1">MSLSAGVLPVLMFVQHASCDVVLTQSSSEIGKPGQSLRLSCAVSGYSLTDSSYATGWIRQPSGKGLEWLALIYYDADTSYADSIKGRFTISKPGSSVYLQMNSLQSDDTAVYYCARRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWL1</accession>
    <name>W5LWL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWL1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000518"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000519.1">
        <property type="protein sequence ID" value="ENSLOCP00000000518.1"/>
        <property type="gene ID" value="ENSLOCG00000000472.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S02J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="HOGENOM" id="CLU_010468_0_3_1"/>
    <dbReference type="InParanoid" id="W5LWL1"/>
    <dbReference type="OMA" id="EQDNDIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000472">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002274">
        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF167">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="43"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="274"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="344" mass="39255" checksum="FD2179D592B92758" modified="2014-04-16" version="1">MWYQEHEIKLESLWKKSSDTSSDKKLCMNEKDPAVEPRLQPRPSELRLVLLGRTGAGKSAAGTTILGSEEFPSEASSSTVTQESQKRTGQLSGRRVTVVDTPDWLHTGLSEGDRRRDVGLCVNLSALGPHTFFLVTPLGRSTGEEKRTLEIFGEKALEHTIILFTHADELTSMTLEESVHTGSRLLEKCGNRYHALNNKDRGSTQVTELLEKIEELVAGNKGSYYSTEMYQEAESQKEREEERLREKQQKKLQNFLRRMKEEIQTREEKIKALEERLREESAGGGAEERERGEREAAERDGGSELGAGEGEERERGDTHRREMEELRQHYEEKAREEAERHLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWP1</accession>
    <name>W5LWP1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000548"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000549.1">
        <property type="protein sequence ID" value="ENSLOCP00000000548.1"/>
        <property type="gene ID" value="ENSLOCG00000000496.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="HOGENOM" id="CLU_002678_16_3_1"/>
    <dbReference type="InParanoid" id="W5LWP1"/>
    <dbReference type="OMA" id="EKMKCHT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000496">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002281">
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002460">
        <property type="entry name" value="Zgc:174574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001927">
        <property type="entry name" value="Zinc finger protein 1184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="581"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="637"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="693"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="721"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="760" mass="85691" checksum="03B2AA637C4DE9F9" modified="2014-04-16" version="1">MEAQLDPEIVFVGRVISSCFLAVCSNSRVTAAAVLALMANRPCRKMASCVLILETELHSFMEVLLKSIVHEVSEVFRNRMSDFEDEFQDKLRSVGRVLVRRAVLRITQCVEDSVGGEIAQMKKENESLKCRLQLWEKEPGAGGDRGQRDRVGYTLPREVTAGIKAEMDTEPELPGSEVSALPDAGERAFLGQQPSEEEMGSTLMQETELTAAGGKETLSAQHTESGQSGEDLDSVPRMKTETESETPGLSVSDDFAEKYFNLDMKNVAKQHFNEQKSVSAQELKEEEMDVLNLAEQDMRPQLIYTKEQRCDVDGKDDITELQHAERGQYRGDLQREKPPRVRKSQPRPSTVEMEKLSPQHRQQQHFCPLNTSDSHQRIPAGVKPFSCSQCGKSFSQLGNLKIHQRIHTGERPFSCSQCGKSCSTSGNLKIHQRTHTGERPFSCSECGKSFSSSCTLKKHQRTHTGERPFSCSQCGKSFSNSSHLKTHQRIHTGDRPFSCSQCEKSFIELRKLKRHQLIHSGEKPFICSQCGKRFIDLTRLKRHQFTHTGDRPFSCSECGKSFMDLTQLKRHQLTHTGERPFICSQCGKSFSQVGTLKNHLRTHTGEKPFGCSECGRGFIDLTQLKRHQLIHTGERPFSCSQCGKSFRQLGSLNTHQRTHTGEKPFGCNQCGKSFSQLSNLKTHQRTHTGGERFSCSQCGKSFIDLTQLRGHQLTHTGDRPFSCSQCGRSFRQLGSLKRHLHVHEEQKLFSCRVGRVYQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWP4</accession>
    <name>W5LWP4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWP4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000551"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000553.1">
        <property type="protein sequence ID" value="ENSLOCP00000000551.1"/>
        <property type="gene ID" value="ENSLOCG00000000501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166734"/>
    <dbReference type="HOGENOM" id="CLU_1363171_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWP4"/>
    <dbReference type="OMA" id="VHHASIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000501">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF14">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="23088" checksum="B77FE30A67EF1BBB" modified="2014-04-16" version="1">RMKACLRSQHDNMEQNKNFETEMEELVPKREVHHASINCIACQVSCHAGCFLPPADEIKDCTVMDDTGHCVICPGRCHHSDTYPRYEVVKVKKTVTELKDNFVKASSKLMSTKEMLEKIEVDFEVIKDRIMELIRLSSSCLARLEEIALKPNALSTAAYIELLIRNEEEEARPDFEDRIAKLRKMKQHAEILAKIANNEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX54</accession>
    <name>W5LX54_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="Ensembl" id="ENSLOCT00000000714.1">
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        <property type="gene ID" value="ENSLOCG00000000644.1"/>
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    <dbReference type="OMA" id="TXSITAP"/>
    <dbReference type="Proteomes" id="UP000018468">
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    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000644">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="41"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="195" mass="19385" checksum="76D8C5A6B7BCC095" modified="2014-04-16" version="1">AVSLFGGIDVVAPSAVFSKIQKLGGTINYAVVLLTIHSHSAQTTTEAPTALTSTTLPTLPSTTDSTSLTSTAPPTHTPTAPPTHTPTISPTHTPTAPPTHTSTAPPTHTPTAPPTHTSTAPPTHTSTAPPTHTSTGSSTHSSTAPPTHTSTASPTHTSTASPTYTSTPPPTPTSTAPPTPTSTAPPTPTSTAPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYJ5</accession>
    <name>W5LYJ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LYJ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001202"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001206.1">
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        <property type="gene ID" value="ENSLOCG00000001072.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="W5LYJ5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001072">
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    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847:SF7">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="61"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="121" mass="13419" checksum="96AD708E96EA2CDB" modified="2014-04-16" version="1">MNPSLSVQCSCTGVSSQCVCMNPSLSVQCSCTGVSSQCVCINPSLSISVYDVLTGCVVSRLSDHDACVRDVSWHPYEANIISSSWDGGVRLWEHRQSHLFDEERERERAPDMEEEEEGETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ88</accession>
    <name>W5LZ88_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein 15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ArhGAP15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Rho-type GTPase-activating protein 15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011661">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ88"/>
    <dbReference type="FunCoup" id="W5LZ88">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001445"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001450.1">
        <property type="protein sequence ID" value="ENSLOCP00000001445.1"/>
        <property type="gene ID" value="ENSLOCG00000001273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685617"/>
    <dbReference type="KEGG" id="loc:102685617"/>
    <dbReference type="CTD" id="55843"/>
    <dbReference type="eggNOG" id="KOG1450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182860"/>
    <dbReference type="HOGENOM" id="CLU_015883_1_0_1"/>
    <dbReference type="InParanoid" id="W5LZ88"/>
    <dbReference type="OMA" id="DHNQWED"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001273">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13233">
        <property type="entry name" value="PH_ARHGAP9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04403">
        <property type="entry name" value="RhoGAP_ARHGAP27_15_12_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000003">
        <property type="entry name" value="Putative rho GTPase-activating protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF108">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="84"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="278"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
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        <source>
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    <sequence length="470" mass="53163" checksum="0E4BCE99A521BB43" modified="2014-04-16" version="1">MDIHSSSHKPVVEKLSTSHHSVGAVQMRIKSSTTNCDRLSQTKSMVLPDPGQPQKPISRHRRNLSQHNLIDITAGEQSNPGKGEYLNKAKIADGGKKTRKNWTTTWVVLDGSKLLFYKDSKQQAVTNLKPGCKPESVDLYGARIEWAADKSSRKNVFLITTGSGNEFLLQAESYSTISKWHDAIGKAIECESKSGKVHSSIRKSSSTEFLNRCGSESPVSKEHKPEHRRSLIFKLNYSASDSADKNGVKNRLKKFISRRPSMKTLQEKGIIKDQVFGCHLITLCEREGTTTPTFVKQCVEAVEKRGLDADGIYRVSGNLSTIQKLRFIVDQEEKLNLDDSQWEDIHVVTGALKMFFRELPEPLFPYRFFEPFVEAIKIRDYNQRVQAVKKLLYQLPRPHHDTMKLLFRHLQKVIAQASKNLMSTQGLGIVFGPTLLWPELDSGNMAVDMVYQNQIVELILTECTEIFGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M166</accession>
    <name>W5M166_LEPOC</name>
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        <name type="scientific">Lepisosteus oculatus</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3V0</accession>
    <name>W5M3V0_LEPOC</name>
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        <submittedName>
            <fullName evidence="11">Fli-1 proto-oncogene, ETS transcription factor</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="ENSLOCG00000002563.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF161">
        <property type="entry name" value="FRIEND LEUKEMIA INTEGRATION 1 TRANSCRIPTION FACTOR"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ETSDOMAIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PNT" evidence="10">
        <location>
            <begin position="117"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="284"/>
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        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="275"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
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        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="455" mass="51077" checksum="0E1AB4B5C2C8F053" modified="2014-04-16" version="1">LFSSIEMICKVLSDEALSVVSDDQSLFEPPYAAVPLPKPDMTASGTQDYGPPHKINPLTPQQDWINRVNVKREYDHLNGSSRESPVDCSVGKCGKLVGGAEATQINYGSYMDEKNGPPPPNMTTNERRVIVPADPSLWSQDHVRQWLEWAIKEYGLLEIDTSLFQSTDGKELCKMSKEDFLRLTSVYNAEVLLSHLNYLRESSSSLSYNTPHTDQSPRLTTKEDPSYDSARRTGWSNSMHSGKGSPAVTQNVSKTSEQQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANAGCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDLAYVPSYHAHQQKVNFVSPHPPSMPVTSSNFFGPPSPYWSSPAGGIYPNPSVPRHPNTHVSSHLGSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M557</accession>
    <name>W5M557_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spermatogenesis-associated protein 2 PUB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M557"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003515"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003522.1">
        <property type="protein sequence ID" value="ENSLOCP00000003515.1"/>
        <property type="gene ID" value="ENSLOCG00000002985.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S1TH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063956"/>
    <dbReference type="HOGENOM" id="CLU_477000_0_0_1"/>
    <dbReference type="InParanoid" id="W5M557"/>
    <dbReference type="OMA" id="CWYRNPV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002985">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048839">
        <property type="entry name" value="SPATA2_PUB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326:SF7">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2/TAMOZHENNIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21388">
        <property type="entry name" value="SPATA2_PUB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 2 PUB-like" evidence="3">
        <location>
            <begin position="60"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="572" mass="64352" checksum="806CE37963F4DBF0" modified="2014-04-16" version="1">MSGAGRKDLCETYRKSLEARVQEGRADLVCRDASLVDAVEGLLRRGGQEVHGSLQNDAFQVIGAALQTAPSLQRGLERLVKGFEVLELAAINLFLCPWRQEFKMIKTFSGVYTHYLKPAFPEQTLLELFQKLGYRPRDRHQLEMGRQPPAEALLQLACGFFAARCESALLLAAASQLEEPRLSAGDLLQERQRCRSLEEVVGNLRRKTEALAKRDENDWRTGGRSEPKTELDLYTEDGRLPNGGDQQQAISKEKPSAVSSVKTGLELEARYSQSAKSTTPSKGERYSDGKGHDSQSFTLCGCSKSYDGVYFLKCPVCKVFHCCGCHVLEECRTKKHEPGLATDAETTVIQQELLKLKLDEKTSPPSDTHHLVCRASSPPLCVCESCHTIHDCSCNACLKQHHELHIVNDRIKQEEWLRKLDAKQWENHSCLSSSGHVGFMCETCHLSHDILCRETKTCASKSHKIFYLPDQTQLKEMTEKEQRYHMHQCITANPQMACQTCFELHGFSCAKLETCKRQHTVRQLGKCTASEKCCNPAYIICMNCCAVFCKNCWFRYPINCTCGHPFGINTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5J6</accession>
    <name>W5M5J6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transforming growth factor beta regulator 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5J6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003654"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003661.1">
        <property type="protein sequence ID" value="ENSLOCP00000003654.1"/>
        <property type="gene ID" value="ENSLOCG00000003093.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013374"/>
    <dbReference type="HOGENOM" id="CLU_037126_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5J6"/>
    <dbReference type="OMA" id="SINFEAY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003093">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056515">
        <property type="entry name" value="INO80E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040092">
        <property type="entry name" value="TBRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715:SF0">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05066">
        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24237">
        <property type="entry name" value="INO80E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00541">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51913">
        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="5">
        <location>
            <begin position="55"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="272"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="553" mass="60120" checksum="286DC34EC9A602A3" modified="2014-04-16" version="1">MDPLNTLSNFEAEMEPDDQGSYSLFPALDNMTSLPGTSETLESDPPSDVADKPNLTWLDAAQIVLEEAGRPMHIKEIKQRIIDRGLVQSNAKSSLEAVMYRETQKGSRRFKRIENRNGVFALLTDDERQQALRAFTAQSFLTAPGQPAFSGSGSGGPLPAFPSPASLSEAKPKVKKLLRKNQNEKYRLKYLRLRKAARAMIFENAALCDEVAHLEEKFVRAKEERRFLLKSLLQYQSLSEGELHSAPSASSHAPASFSSAAGGAAVLGASPGLAPAASGGDEGTSKKSKKDRKERARENGREDALRKVSKKKKLAEGGSRKLVQPIPLDSSGRPVFPIVLGGLTVYSLGEIITDRLHFHDENAIYPVGFCSTRMFASMKSPEQQCLYTCQIKDGGSGPQFEIVPEEDPQNAIVASSALTCHANLLKAIAAMRGKPLTSIVPSGADFFGFSHPTIQNLIQSCPGARKCANYQWVRFDVWRPGDGQLPHSLSEDDASLNFEAFQRQQGFEGSLALSSLQVRPPQPPLSSHARLLGQQHLGPSPLQPSASASHFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5R4</accession>
    <name>W5M5R4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphodiesterase</fullName>
            <ecNumber evidence="16">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="16">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="16"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_006642656.1">
        <property type="nucleotide sequence ID" value="XM_006642593.3"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000003722"/>
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        <property type="gene ID" value="ENSLOCG00000003151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694452"/>
    <dbReference type="KEGG" id="loc:102694452"/>
    <dbReference type="CTD" id="5140"/>
    <dbReference type="eggNOG" id="ENOG502QSV8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159336"/>
    <dbReference type="HOGENOM" id="CLU_008844_1_0_1"/>
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        <property type="component" value="Linkage group LG27"/>
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    <dbReference type="Bgee" id="ENSLOCG00000003151">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="P:negative regulation of cAMP/PKA signal transduction"/>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="102"/>
            <end position="129"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="171"/>
            <end position="191"/>
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            <begin position="197"/>
            <end position="217"/>
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        <location>
            <begin position="636"/>
            <end position="1057"/>
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            <begin position="380"/>
            <end position="461"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="562"/>
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            <begin position="613"/>
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            <begin position="1002"/>
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            <begin position="1060"/>
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        <location>
            <begin position="409"/>
            <end position="426"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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            <begin position="429"/>
            <end position="441"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
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            <begin position="619"/>
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            <begin position="1002"/>
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            <begin position="1067"/>
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            <begin position="1082"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
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    <sequence length="1094" mass="123062" checksum="B3F5250B6F7F9973" modified="2014-04-16" version="1">MAVREEGEVLHSKIKHHDGVGNANSTESFRNGYVKSCVTPLKQDHQRGFLFNVCRFCNADLIGSRRFRASALYFGAIAVFAASLLISRSLQSGPSSSLLRTLQSFLLVFSQSVSPLFSIGCAFFFLTFYLRRKKRESYRCWLVSLPASCYLGDFLVLQFLHWEDNQHQMQMVGRLLFVLGCVGILTLIPTLKLRYSVLILVFASLVWLVSFTSLECLPKPLRPLLACLAGVSGSLLALCFDHYYLPREAPHRIANAEEKVPVIRPRRRSSCVSLGETSTSYYGSCKMPRRPSLPCISREQMILWDWDLKQWYKPQYQGAASGNGVDLSVINEARNMVSDLLADSSLSPQVIASLRSISSLMGTFSGSCRPRVNPFTPFPGFYPCTEMEDPTEKGERKPHKGMNSRNSLPTQQLRRSSTSSSTLPSLEAARWERTNGKRPHQEFSAMSPGPQPNGPSNANLLTIPKQRSSSVTLTHHVTRRAGTSPSLSPVNSPSHSPTGGSGSLVTRSPVEFPDTADFLTKPSVNLHKPLGYALSSPDFQQQFRTSSIPLCTSCGRQMLKTVPSAESGEANLLSATETQRKRSGEGTESPCDSSQIKEAYEKAETQCEKYPDIRESDNESQTDTEQQQCQTHTSQETESNQDFILDPLLQDHEVLMERINNWNFPIFDLVDKTGGKSGRILSFVSYTLFQDTGLFEIFKIPVREFMSYFCALENGYRDIPYHNRIHATDVLHAVWYLTTRPIPGFQQIHNDHITGSDTDSDSGISPGRVAYASSKSCVITDDSYGCLSWNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWSLYLSQPEFNFLVHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNTKVNDVNSPGIDWTNENDRLLVCQVCIKLADINGPAKGRELHLKWTEGIVNEFYEQGDEEASLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGHWIDEESSEDVEDGEESETEDDDLEEDIEPKRRKTRRRLFCNIMHHLTENHKVWKKTIEEEEKSKVELSKQQIENSSLPQTSDEIQVIEEETEEEERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M642</accession>
    <name>W5M642_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000003850.1"/>
        <property type="gene ID" value="ENSLOCG00000003259.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004856"/>
    <dbReference type="HOGENOM" id="CLU_129470_0_1_1"/>
    <dbReference type="InParanoid" id="W5M642"/>
    <dbReference type="OMA" id="RIVHHSG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003259">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000166">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="132" mass="14804" checksum="05E08E32F7F2C523" modified="2014-04-16" version="1">RVSLFLLQPPKDDKKKKDAGKSSKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLYKEVPNYKLITPAVVSERLKIRGSLARAALQELLNKGMIKLVSKHRAQVIYTRNTKGGDAPGDEKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6C0</accession>
    <name>W5M6C0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pinhead</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6C0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003928"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003936.1">
        <property type="protein sequence ID" value="ENSLOCP00000003928.1"/>
        <property type="gene ID" value="ENSLOCG00000003323.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005847"/>
    <dbReference type="HOGENOM" id="CLU_075324_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6C0"/>
    <dbReference type="OMA" id="NQHGQIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003323">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39313">
        <property type="entry name" value="IM:7138239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39313:SF1">
        <property type="entry name" value="IM:7138239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865619" evidence="1">
        <location>
            <begin position="30"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="326" mass="35768" checksum="E2917100D9ADC071" modified="2014-04-16" version="1">CRSFAMDVLSTSLLVTLCFLCPAITGVFGRHVGQRNLFTERGCCRRQSHFIYVGQDVSGSPISVDVGLCRSHCGGYQGASGSYGSGIPGYSKHTSLLEFLRSKKSQRERQPSPEAPEPAGLVASCSAGAACVSAGVRVERVLLFDGVREVEVTEECHCEPSPPECVRMPALKTYFFETPYETVVDVGKCSKPPGTPDGISCVPTKFSSALVETPSKMELIQTAAACDLRENCYRVSYKEYYYEVFYNSNGVKEEKLKEIDVGRCLGSCTSGNRCLLRDSSPQEQCRVWAERPARSCVPRDFDSHAFRSRHGQVRTVLSITSCACRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6G9</accession>
    <name>W5M6G9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Extended synaptotagmin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the extended synaptotagmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6G9"/>
    <dbReference type="FunCoup" id="W5M6G9">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003977"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003985.1">
        <property type="protein sequence ID" value="ENSLOCP00000003977.1"/>
        <property type="gene ID" value="ENSLOCG00000003337.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165191"/>
    <dbReference type="InParanoid" id="W5M6G9"/>
    <dbReference type="OMA" id="WANKVIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003337">
        <property type="expression patterns" value="Expressed in zone of skin and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08391">
        <property type="entry name" value="C2A_C2C_Synaptotagmin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000025">
        <property type="entry name" value="Extended synaptotagmin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000093">
        <property type="entry name" value="Extended synaptotagmin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000114">
        <property type="entry name" value="Extended synaptotagmin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037733">
        <property type="entry name" value="Ext_Synaptotagmin_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF4">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="18">
        <location>
            <begin position="150"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="327"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="465"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="747"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="626"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="883" mass="99192" checksum="183EDA74AE593E94" modified="2014-04-16" version="1">MSRLETSRGAPRHPAGGSGGVSETGSGGPRSPADSSQGGSPKPAAADDAGSESLEDTSYSTSAVNQMLMEVLLYFGRAFVFLFPVYLTGYLGLSISWVLLCLMLWTWWRRNRKVKSTRLDTAIDFLENERFVINEEMKALNMPAWVNFPDVERADWLNKVLQQAWPFFGIYMEKLLKEKIQPSIRASSPHLKTFTFTKVHFGQKPLRVTGVKAYTEEVDKRQVILDLNLSYAGDVDIDAEVKKPITAGVKGIQLTGLLRVILEPLTGQSPLVGGVTIFFIRRPQLDINWTGLTNLLDSPALNNISEDAILNIISSFMVLPNRMCFPLIDQVQVAQMRFPLPRGVVRVHLIEAQDLMAKDTYMLGMVKGKSDPYAKLRVGTQAFKSKTIKENLDPRWGEVYEFVVHEAPGQELEVELYDEDTDRDDFLGRMKLDLGDVMKERLLDEWFELEEAETGKLHLRLEWLSLVTDLEKLEETREGRACAMLAVYLDSASSLPRNLTEYSGTDYGGKHNKQAKQSKVTRKNDNPSSYAELTVEQQTQKSNTIHATKDPVWEECFTFFVHHIQNQSLIVQIKGSDKKVPLGVLALPLGRLLTATDMTLDQRFQLERSGVNSFLKMKATLRFLAHEKPQPKKVDPKLNARARGPAKGANKPSSPLPSPSEPLPSPDREVRSPLGGESGRKAPSLRPNGQSEGKPTAPATPFVRDVSSVHRYDSSSMLSSYSVASSRYDLTEGGGSYPEAILSHQGGFGEIQLSVRYAALRKQLLVLVNCCRNLVSCGDQGSDPYVRIYLLPDRKWKHRKRTLVKKKTSDPVFEEKFEFSVSLEEAKTRQLDVAVKNNRTYKKRDRKEIGMVLIDLSQDDLRKGFTNWYELSLPGVQNKGWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6Y6</accession>
    <name>W5M6Y6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipid scramblase CLPTM1L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cisplatin resistance-related protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleft lip and palate transmembrane protein 1-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Scramblase that mediates the translocation of glucosaminylphosphatidylinositol (alpha-D-GlcN-(1-6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol, GlcN-PI) across the endoplasmic reticulum (ER) membrane, from the cytosolic leaflet to the luminal leaflet of the ER membrane, where it participates in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a lipid glycoconjugate involved in post-translational modification of proteins. Can also translocate 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) (phosphatidylinositol or PI), as well as several other phospholipids (1,2-diacyl-sn-glycero-3-phosphocholine, 1,2-diacyl-sn-glycero-3-phosphoethanolamine), and N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = 6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71495"/>
            <dbReference type="ChEBI" id="CHEBI:190691"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71491"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M6Y6">
        <property type="interactions" value="1274"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004144"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004152.1">
        <property type="protein sequence ID" value="ENSLOCP00000004144.1"/>
        <property type="gene ID" value="ENSLOCG00000003491.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063461"/>
    <dbReference type="HOGENOM" id="CLU_019907_4_1_1"/>
    <dbReference type="InParanoid" id="W5M6Y6"/>
    <dbReference type="OMA" id="TTMWRAF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003491">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="536" mass="62609" checksum="B019BB0B420FBFBA" modified="2014-04-16" version="1">MFQKTSFTTLIVGVFVVYVLHTCWVMYGIVYTKPCEKIQNENCITSYLAEKPRLQLSIYTSLKPTGDGNQNLIFSMEDFEVFSKFERKINVSIPKKTRNNGTLYAFVFVHKAGVSPWHDSKQVHHIAQLTTYMVPKPQEINLIIGEDTSQQSEPQKKTNGLQDRPVSHWRSRLTLNVLSEDFAFDREALPSDVHRYMRVIQYGKRMLYLPILFIDELSNRVKDLMVINSSTTELPLTISYDTISLGKLRFWIHMQDAVYSLQQFGFTEKDADEIKGIFVDTNLYFLALTFFVAAFHFFFIFCFLNDVXFFSHQKTQVKTTGNAVLWRCFSTIVIFLYLLDEQTSLLVLIPAGIGSIIEIWKVKKAFKIHVLWKGLKPTFQFGKSDESERKTEEYDTQAMKYLSYLLYPLCIGGAVYSLIYVKYKSWYSWLINSLVNGVYAFGFLFMLPQLFVNYKLKSVAHLPWKAFMYKAFNTFIDDVFAFIITMPTSHRLACFRDDVVFLIYLYQRWLYPVDKSRINEYGVSYDEKPKGKPHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7X8</accession>
    <name>W5M7X8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Far upstream element (FUSE) binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7X8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004486"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004494.1">
        <property type="protein sequence ID" value="ENSLOCP00000004486.1"/>
        <property type="gene ID" value="ENSLOCG00000003745.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160043"/>
    <dbReference type="InParanoid" id="W5M7X8"/>
    <dbReference type="OMA" id="QPWGPYG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003745">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22481">
        <property type="entry name" value="KH-I_FUBP1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22484">
        <property type="entry name" value="KH-I_FUBP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22487">
        <property type="entry name" value="KH-I_FUBP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000008">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015096">
        <property type="entry name" value="FUBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048250">
        <property type="entry name" value="KH-I_FUBP1_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048251">
        <property type="entry name" value="KH-I_FUBP1_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048252">
        <property type="entry name" value="KH-I_FUBP1_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09005">
        <property type="entry name" value="FUBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="207"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="298"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="397"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="469"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="569"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="569"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="584"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="636"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="688" mass="72944" checksum="77BCECFBB37C80A9" modified="2014-04-16" version="1">MADYSSVAPPSSGSGAGMNDAFKDALQRARQIAAKIGGDTGGAPPPATNEFGYPGQKRPLEDAGEFIAPMLRSVECLEAWVIGIYIKSRTRIKVSGPFSSYIIEIPYKAYTSPMGGMNAPQRSVSEEFKVPDGMVGFIIGRGGEQISRLQQESGCKIQIAPDSGGLPDRSVMLTGSHDSIQTAKRLLTEIVEKGRPGPVFHHSDGPGMAVQEILVPASKAGLVIGKGGETIKQLQERAGVKMVMIQEGPQNTGADKPLRISGDPFKVQQAKEMVMELIRDQGGFREQRNEYGSRMGGSDCSLDVPVPRFAVGIVIGRNGEMIKKIQNDTGVRIQFKPDDGSTPERIAQIMGPPDRSQHAAEIITDLLRSVQAGGPPGPGGRGRGRGQGNWNMGPPGGLQEFSFTVPTMKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPSLKMFTIRGSPQQIDYARQLVEEKIGGPVSPLGGPHGPPGPHGGPAPHGPPGPPGPPAPMGPYNPGPYNQGPPGPHGPPAPYQPQGWGNGFPHWQQGQQDPGKAAADANAAAWAAYYSQYQQQPQAPIAAASAAPGSAQTNGQSDQQNPGQSGQADYTKAWEEYYKKLGQQGQQPQDYTKAWEEYYKKQDPKGQAAPAAAAATQAGGQPDYSAAWAEYYRQQAAYYGQASPQAMPPHPPAPQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M827</accession>
    <name>W5M827_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hematopoietic cell signal transducer</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNAX-activation protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Membrane protein DAP10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019138">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M827"/>
    <dbReference type="FunCoup" id="W5M827">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000004543.1">
        <property type="protein sequence ID" value="ENSLOCP00000004535.1"/>
        <property type="gene ID" value="ENSLOCG00000003804.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SG5F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205757"/>
    <dbReference type="HOGENOM" id="CLU_193379_0_0_1"/>
    <dbReference type="InParanoid" id="W5M827"/>
    <dbReference type="OMA" id="NAHKVYM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003804">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009861">
        <property type="entry name" value="HCST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21409">
        <property type="entry name" value="HEMATOPOIETIC CELL SIGNAL TRANSDUCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21409:SF1">
        <property type="entry name" value="HEMATOPOIETIC CELL SIGNAL TRANSDUCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07213">
        <property type="entry name" value="DAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="53" mass="6000" checksum="C5D2B82E4A2B2AD4" modified="2014-04-16" version="1">CYKIEPGTMVGIIAGDVVLTVLIVISVYYCASRKRHKREQADKVYMNVRANVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9W1</accession>
    <name>W5M9W1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Muscle segment homeobox 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Msh homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9W1"/>
    <dbReference type="FunCoup" id="W5M9W1">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005170"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005178.1">
        <property type="protein sequence ID" value="ENSLOCP00000005170.1"/>
        <property type="gene ID" value="ENSLOCG00000004331.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0492">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164982"/>
    <dbReference type="HOGENOM" id="CLU_072675_0_0_1"/>
    <dbReference type="InParanoid" id="W5M9W1"/>
    <dbReference type="OMA" id="MAPYPFA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004331">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000134">
        <property type="entry name" value="Homeobox protein MSX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050674">
        <property type="entry name" value="Msh_Homeobox_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="108"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="232" mass="25864" checksum="6C555ED49EF67D44" modified="2014-04-16" version="1">KSKTPSLPFSVESLISDRTACRTDHVSTEPVLVPPKPVGEQEGPHSPRRGYAEKTRSVDSFPNSTGCPKSETEDLHDSDQSTWFQTSSFSTPPTGHSSPPPCTLRKHKNNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSNSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKLATKPLLPAFAFPFPLGAHVGSPSLYGPSNAFHRPALPVPGLFSGPVTYGMYYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA56</accession>
    <name>W5MA56_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SWI/SNF related BAF chromatin remodeling complex subunit C1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031624">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA56"/>
    <dbReference type="FunCoup" id="W5MA56">
        <property type="interactions" value="1786"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005265"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005273.1">
        <property type="protein sequence ID" value="ENSLOCP00000005265.1"/>
        <property type="gene ID" value="ENSLOCG00000004394.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156347"/>
    <dbReference type="InParanoid" id="W5MA56"/>
    <dbReference type="OMA" id="LLLLEXE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004394">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071564">
        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003206">
        <property type="term" value="P:cardiac chamber morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049898">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032450">
        <property type="entry name" value="SMARCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032448">
        <property type="entry name" value="SWIRM-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16496">
        <property type="entry name" value="SWIRM-assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16498">
        <property type="entry name" value="SWIRM-assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52032">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="28"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="9">
        <location>
            <begin position="449"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="617"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="622"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="744"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="960"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="768"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="822"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="832"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="962"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1025"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSLOCP00000005265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1085" mass="120763" checksum="3A968CA353568805" modified="2014-04-16" version="1">MATAAIAAGGAGSGGPAAGQGGSGASTLSVGRKKDGGPSAKYWESSETVSQLESVRLWIGKHYKKYVQADSPSNKSLAGLVVQLLQFQEDAFGRRVNNPALTKLPAKCFLDFKPGGALCHILGSAYKFKSEQGWRRFDLQNPSRMDRNVEMFMTVEKTLVQNNCLTRPIVYVLPDIEPKQANKLKDIIKRHQGSITEDKTKATHHIYPTPPSLDEEEWLRPVMRKDKQVLVHWGMYPDSYDTWVSASDVDGEVEEPPNPEKPWKVHAKWVLDTDSFNEWMNEEDYEVDESKKPVSFRQRIFMRDEESSRTPDRKDRKAAASGKKRRRSPSPPPTPVESRKKGGKKGHPGPYWKRRGHREEEEPEEDLTKDMEDPTPVPNMEEVTLPKNVNPKKDSENTPVKGGTVADLDEQEDEPMVTGGKEEEEQGKAEVNRLIDSSEDNVTEQTHHIIIPSYASWFDYNCIHEIERRALPEFFNGKNKSKSPEIYLAYRNFMIDTYRLNPQEYLTSTSCRRNLTGDVCAVMRVHAFLEQWGLINYQVDAESRPLPMGPPPTPHFNVLADTPSGLVPLHHRPPQVPASQQMLNFPEKGKEKPTDLQNFGLRTDIYAKKNPKSKGGSAGREWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLENSDASLGPLAYQPVPFSQSGNPVMSTVAFLASVVDPRVAAAAAKAALEEFSRVREEVPAELVEAHVKKVQEAARSTGRLDPAYGLESSGIAGTSPDEPEKTESTEPEKMETDSDSQQPEKAESKEEVEKPSETTERQPEREKMKNESQECEREEAESEGGEESKTDKEKEESQESGEISKEGEGEDGRKKVEPEVEEGNIATAAAAALASAATKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREKEALEQQRQQLLTERQAFHMEQLKYAEMKARQQMEQQQAAAAQNPGAHHGPPPGMMGHQPPSGFPPMHHPMGSHHPPQTGPMGGVSQPMPGRVMPSAPPPGGPPPPNMPPMMGPRHPGAPNGMFYNYPTPPAGQPDGIAPAPVGPPVAAPSRVAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAG9</accession>
    <name>W5MAG9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">DNA oxidative demethylase ALKBH2</fullName>
            <ecNumber evidence="24">1.14.11.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Alkylated DNA repair protein alkB homolog 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,N(2)-etheno-2'-deoxyguanosine in double-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxyguanosine in double-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70487"/>
            <dbReference type="Rhea" id="RHEA-COMP:17910"/>
            <dbReference type="Rhea" id="RHEA-COMP:17912"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:189586"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:70488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,N(6)-etheno-2'-deoxyadenosine in double-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxyadenosine in double-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70463"/>
            <dbReference type="Rhea" id="RHEA-COMP:17897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17903"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:189583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:70464"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,N(6)-etheno-2'-deoxyadenosine in single-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxyadenosine in single-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70459"/>
            <dbReference type="Rhea" id="RHEA-COMP:17896"/>
            <dbReference type="Rhea" id="RHEA-COMP:17904"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:189583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 3,N(4)-etheno-2'-deoxycytidine in double-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxycytidine in double-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70467"/>
            <dbReference type="Rhea" id="RHEA-COMP:17070"/>
            <dbReference type="Rhea" id="RHEA-COMP:17905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:189585"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70468"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 3,N(4)-etheno-2'-deoxycytidine in single-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxycytidine in single-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70471"/>
            <dbReference type="Rhea" id="RHEA-COMP:12846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17906"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:189585"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:70472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:30299"/>
            <dbReference type="Rhea" id="RHEA-COMP:12192"/>
            <dbReference type="Rhea" id="RHEA-COMP:12193"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:32875"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="1.14.11.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:30300"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>an N(1)-methyl-2'-deoxyadenosine in double-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in double-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70443"/>
            <dbReference type="Rhea" id="RHEA-COMP:14236"/>
            <dbReference type="Rhea" id="RHEA-COMP:17897"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:139096"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:70444"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an N(1)-methyl-2'-deoxyadenosine in single-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in single-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70447"/>
            <dbReference type="Rhea" id="RHEA-COMP:17895"/>
            <dbReference type="Rhea" id="RHEA-COMP:17896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:139096"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:70448"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N(3)-methyl-2'-deoxycytidine in double-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxycytidine in double-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70439"/>
            <dbReference type="Rhea" id="RHEA-COMP:14237"/>
            <dbReference type="Rhea" id="RHEA-COMP:17070"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:139075"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70440"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an N(3)-methyl-2'-deoxycytidine in single-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxycytidine in single-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70435"/>
            <dbReference type="Rhea" id="RHEA-COMP:12846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17894"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:139075"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:70436"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with PCNA homotrimer; this interaction is enhanced during the S-phase of the cell cycle. Interacts with nucleolar proteins NCL, UBTF and NPM1. Interacts with XRCC5-XRCC6 heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.33" evidence="24"/>
    <dbReference type="EMBL" id="AHAT01005109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAG9"/>
    <dbReference type="FunCoup" id="W5MAG9">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005378"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005386.1">
        <property type="protein sequence ID" value="ENSLOCP00000005378.1"/>
        <property type="gene ID" value="ENSLOCG00000004502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694971"/>
    <dbReference type="KEGG" id="loc:102694971"/>
    <dbReference type="CTD" id="121642"/>
    <dbReference type="eggNOG" id="ENOG502QTDK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159009"/>
    <dbReference type="HOGENOM" id="CLU_048788_5_0_1"/>
    <dbReference type="InParanoid" id="W5MAG9"/>
    <dbReference type="OMA" id="TQHHWQH"/>
    <dbReference type="OrthoDB" id="445341at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004502">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051747">
        <property type="term" value="F:cytosine C-5 DNA demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000004">
        <property type="entry name" value="DNA oxidative demethylase ALKBH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032852">
        <property type="entry name" value="ALKBH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31573:SF1">
        <property type="entry name" value="DNA OXIDATIVE DEMETHYLASE ALKBH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="30">
        <location>
            <begin position="155"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00053025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00062909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00072134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00077989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00081727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR632852-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="277" mass="31427" checksum="FE95115B69E88B0C" modified="2014-04-16" version="1">MDRFVTRTKKRPATATAVAVATHNELETTDERKRTRCAVSGDEEEYDCPSEGEFSAPLPWQKIEAEALDLDYTVLFSKAEADRLFKKLEEEIEYFSGEKAKVQVFGKFYNIPRKQATYGDPGLMYSFSGVHHLARPWTPVLEYIRDCVTKATGHTFNFVLVNRYRDGGDHMGEHRDDERELDPACPIASVSFGAARDLVFKHQDARGRRAPAPRIAPVRLELQHGSLLLMNPPTNARWYHSLPVRKRVPLPRVNLTFRRILRDGRGRAAPAGHSAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB45</accession>
    <name>W5MB45_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane 6 superfamily member 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TM6SF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639918.1">
        <property type="nucleotide sequence ID" value="XM_006639855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB45"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005604"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005612.1">
        <property type="protein sequence ID" value="ENSLOCP00000005604.1"/>
        <property type="gene ID" value="ENSLOCG00000004678.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683473"/>
    <dbReference type="KEGG" id="loc:102683473"/>
    <dbReference type="CTD" id="791179"/>
    <dbReference type="eggNOG" id="ENOG502QRB2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012913"/>
    <dbReference type="HOGENOM" id="CLU_046717_0_0_1"/>
    <dbReference type="InParanoid" id="W5MB45"/>
    <dbReference type="OMA" id="VQMLMYM"/>
    <dbReference type="OrthoDB" id="8181520at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004678">
        <property type="expression patterns" value="Expressed in embryo and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21106">
        <property type="entry name" value="TM6SF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047195">
        <property type="entry name" value="TM6SF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059044">
        <property type="entry name" value="TM_Tm6sf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568:SF9">
        <property type="entry name" value="TRANSMEMBRANE 6 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568">
        <property type="entry name" value="TRANSMEMBRANE SUPERFAMILY 6 MEMBER 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26083">
        <property type="entry name" value="TM_Tm6sf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="60"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="216"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="376" mass="43128" checksum="611DA78DF859D962" modified="2014-04-16" version="1">MNLPTEIGVFLLSLSSLGVIYAMNNMPSLQDPVSILGIGAAVLVMLYVLVYYSVRNNSPKDPLFYVFAVFSFTCVIDLIIAAEEDGYVSGFMEFYMKEGEPYLRTAFGIMMCYWDGTVHYFLYLVMVRRVANKQPYRGVGLFWIGSLLVNMIVFVPGIVLGKYGSEIRPAFLLNVPYVLVPMWAAITLFHRPRELPIIPADTIAAEQRKGLLHRPLDMILLLFLLWAMVIAIFRGFVVLECPLDFCFTYIYQYEPYLKDPVVFPKVLMLLYLFYGLPMLAVFTYGLVKPGCTWMLDWTLFFAGGVAQAQWSHIGASLHPRTPFTYRVPEDTWWPVLLLNLLFALGPQLLAWRCHRHPEFFMKNVPQGQTNQEKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCA0</accession>
    <name>W5MCA0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GID complex subunit 8 homolog b (S. cerevisiae)</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCA0"/>
    <dbReference type="FunCoup" id="W5MCA0">
        <property type="interactions" value="1972"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006009"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006017.1">
        <property type="protein sequence ID" value="ENSLOCP00000006009.1"/>
        <property type="gene ID" value="ENSLOCG00000004990.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015162"/>
    <dbReference type="HOGENOM" id="CLU_073203_1_0_1"/>
    <dbReference type="InParanoid" id="W5MCA0"/>
    <dbReference type="OMA" id="KMILWAQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004990">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
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        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="85"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="250" mass="29323" checksum="F12599EF106A1470" modified="2014-04-16" version="1">VVDAKCFICLIIQLFQFFSVFMMSYAEKPEDITKDEWMDKLNNVHIQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSIDLDSLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDNPEDSPFGDLLNMMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGTIEDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCJ5</accession>
    <name>W5MCJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019536">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCJ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006104"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006112.1">
        <property type="protein sequence ID" value="ENSLOCP00000006104.1"/>
        <property type="gene ID" value="ENSLOCG00000005070.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S36D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267394"/>
    <dbReference type="HOGENOM" id="CLU_110531_0_0_1"/>
    <dbReference type="InParanoid" id="W5MCJ5"/>
    <dbReference type="OMA" id="NCEGDEN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005070">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF24">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6 FAMILY MEMBER M2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866034" description="UPAR/Ly6 domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="111"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="20907" checksum="FA01EFD85C3E529E" modified="2014-04-16" version="1">MFNVNLKCLIVFFTCSVATSLSCSACAGTDACTQITCPSAFTQCFTGNISFGGTFTSVKSCTTSNICTGGALSINLGSVRVTENLNCCATDNCNTNTTAPAAYTDTTPSGLKCYYCDGTVCNKTLTCQGVENRCITADLSNQIVKGCASNSVCTATIGDLSQVLGQAFNTTCCSGNLCNNAMRTGQSVFLLLVPLAFIKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCV4</accession>
    <name>W5MCV4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCV4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006221.1">
        <property type="protein sequence ID" value="ENSLOCP00000006213.1"/>
        <property type="gene ID" value="ENSLOCG00000005155.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102690167"/>
    <dbReference type="eggNOG" id="KOG2514">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157182"/>
    <dbReference type="InParanoid" id="W5MCV4"/>
    <dbReference type="OMA" id="WKQRVRT"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005155">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF13">
        <property type="entry name" value="ANOCTAMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="600"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="10">
        <location>
            <begin position="96"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="9">
        <location>
            <begin position="358"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1024" mass="118171" checksum="FFCF4028B9E275E1" modified="2014-04-16" version="1">MDENGTGHRKTSERDDAREACKGCFVHSSSGDSLGKVQQPTEMRRNEKYTPLPIKEKRVDIINIKGIEDIGYVQQDSMILNSLCPEDDTQCGQGLYFHDGKRKVDYILTYHIKKPSGSKKYSSRFSENALARSIRRSRHHPSKKDPEIAAQDLSMDYHEDDKRFRREEFEGNLTEMGLELERDEDTKTPGVGFVKIHAPWNVLCREAEFMKIKMPTKKVYEVKQGSNLVEKINAFINKVTEPLHPNVEENRITTVKHLSYPFSREKQHLFDLSDKDSFFDSKTRSSIVYEILKRTKCTKAKYSMGMTSLLANGVYTAAYPLHDGDTNGENRAPNDRKLLYEEWASYSVFYKYQPIDLIRKYFGEKIGLYFAWLGVYTQMLIPASIIGIIVFLYGCATVDDDIPSMEMCDKRNNITMCPLCDKVCGYWKLSTGCATARASHLFDNPATVFFSIFMALWATMFMEHWKRRQMRLNYKWDLTGFEEEEEAVKDHPRAEYEFRVMQKTLKKEGKFKKKPPEVMEERTNKWKQTVQTAMGGVKLTEKEKLTCRDRMPAYVTNLVMMLLMIGVTFAIVFGVILYRVSTKAALAMSSNPVTRSNVRLTVKATAAIINLVIILILDEVYGAIARWLTLLEVPKTDKSFEERLIFKTFILKFVNAFTPIIYIAFFRGRLVGRPGNYVYAFKSFRMEECAHGGCLMELCIQLSITMLGKQLIQNNLFEIGIPKLKKLIRYIKSKRRPFQEEEREKKLQHYETDYFLDPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPIAARAKDIGIWYNILRGIGKIAVIINAFVISFTSDFIPRLVYQYMYSPDGSMHGFVNHTLSYFNVSHFQEGTEPHIPSNLGYKVEICRYKDYREPPWSPSAYDISKEFWAVLAARLAFVIVFQNVVMFMSDFVDWLIPDIPKDISQQIHKEKILMVELFMKEEQGKLNMLESLVGRGKDPKGMENCNSHSPSPQAHELKGSAVSLNAQNVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MES3</accession>
    <name>W5MES3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1 regulatory subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RNA polymerase II subunit 5-mediating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with POLR2E/RPB5, RUVBL2 and RUVBL1. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts with UXT. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1. Interacts with TSC1 and TSC2. Interacts with PRPF8 and EFTUD2 in a ZNHIT2-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641447.2">
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    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
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    <sequence length="493" mass="55708" checksum="D6AE674C7C7E570A" modified="2014-04-16" version="1">MADKNKRENVLPTEVSRLREEHEKMVSGCQDQIRHWNKVECDYEALQERLSSLPDKLSYDIMVPFGSLAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAINLVEHRKKHVKSALDDLYKVVKNFEARVGFTEDLEKLSGGKDDFVDIREEVENEEILTKGKQRTAHKPNSKPKIQDVFEASIDVEENDSKGRSGILSEEKLWARLDELEAQEELMDQQVRVTDNPYTNGEDTASSSSEEEKECQNMNLTSGLHEREAWPPVTQGDSRLITCQVNGTGQPHSDDDEDVGSFVPTIYFLHTVEQKRVRINTGKNTTLKFSERKEEAKRKRKNSTGNGHSLHELPKIKTPADIYRVFVDVVNGELIPRKSILKSRSRENSVCSDTSESSAADFDDRRGFSRSLSHDEATHSDTSESILEEENSPSKPLPMLGVYEAFSGTVVEKDPILPSVIPHLTISPPALPTIPERKCEEIPPEAPQQPSKRVSKFKAARMQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFA7</accession>
    <name>W5MFA7_LEPOC</name>
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        <recommendedName>
            <fullName evidence="15">Phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="15"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000005849.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
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    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of cAMP/PKA signal transduction"/>
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        <property type="entry name" value="PDEase"/>
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        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0844">Vision</keyword>
    <feature type="domain" description="PDEase" evidence="17">
        <location>
            <begin position="484"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="833"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="560"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>AMP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="858" mass="99132" checksum="3FB201007A4F9917" modified="2014-04-16" version="1">MGELNKESVEKYLENNPQFAKEYFDKKLRAEVLGAAFSDTKVNVKDVASIKEITQIHEATFIFELLKAIQDVSNVEKTMHNVLQRIALLVKADKCSLFMSRSRNGIPELSTTLFNVTATSSFEENIVSPDSEIVFPLDVGIVGWVAHTKKTFNIPDVKKNTHFSDFVDKQTGYTTKNMLACPVMNGKEVLAVVMAINKTDEPEFSKADEELFLKYLNFATVILQQNQISYMYNVESRRSQVLLWSASKVFEELTDIERQFHKALYTVRNYLNCERYSVGLLDMTKEKEFYDEWPIKLGEAEPYKGPKTPDGREVNFYKIIDYILHGTEEIKVIPTPPADHWSLLSGLPTYVAENGFICNMMNAAADDFFTFQKEAFDETGWKIKNVLSLPIVNKKEEIVGVATFYNRKDGKPFDEYDEQITEALTQFLGWSVLNTDTYDKLNKLENRKDIAQEMLMYQTKCTKSELQSILNTQEKFNQEPEDCDSKELVKLLKSNLPDPKEVELLEFHFSDFPVSEFDLIRCGIQCFFVLNVVEKFKVPAEVLTRWMYTVRKGYRDITYHNWRHGFNVGQTMFTLLLTGRLKKYYSDLEAFAMVAAAFCHDIDHRGTNNLYQMKSASPLARLHGSSILERHHLEYSKTLLEDESLNIFQNLQKRQFDTVIHLFEVAIIATDLALYFKKRTMFQKIVDATEQMATEEEQINYVSNNPIRKEIVMKRAMMMTGCDLSAITKPWEVQSKVALMVANEFWEQGDLERTVLEQQPIPMMDRNKSDELPKLQVGFIDFVCTFVYKEFSRFHKEITPMFTGLQNNRVEWKALADVYEEKMKAIEEEKKKLEEASKKVEGGGEVSEGGKSKTCTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGG3</accession>
    <name>W5MGG3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Matrix metalloproteinase-17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Membrane-type matrix metalloproteinase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Membrane-type-4 matrix metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="27">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="27">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGG3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007472"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007480.1">
        <property type="protein sequence ID" value="ENSLOCP00000007472.1"/>
        <property type="gene ID" value="ENSLOCG00000006183.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158699"/>
    <dbReference type="HOGENOM" id="CLU_015489_8_2_1"/>
    <dbReference type="InParanoid" id="W5MGG3"/>
    <dbReference type="OMA" id="WLVCGDP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006183">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000003">
        <property type="entry name" value="Matrix metallopeptidase 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000016">
        <property type="entry name" value="Matrix metallopeptidase 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF330">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="14 26" id="KW-0862">Zinc</keyword>
    <keyword evidence="18" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="31">
        <location>
            <begin position="129"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="29">
        <location>
            <begin position="339"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="29">
        <location>
            <begin position="388"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="29">
        <location>
            <begin position="435"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="29">
        <location>
            <begin position="482"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="active site" evidence="25">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="27">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="28">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00075831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00076283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="596" mass="67927" checksum="4E6BEB101CCB814C" modified="2014-04-16" version="1">LSQKILSNWGQSTTAENQYLLYCQTWPVSCLFLLSLSSPQITKYKNMRSLKDWLSKFGYLPPPDSVTGQLQTKEALTEAIKAMQKFGGLEETGVLDEATLVLMKTPRCSLPDMSDAGMDSGRQKRAASSPTKWYKRHLSWRVRKFPKESHLLGHDTVRALMYYALKVWSDIAPLNFHEVAGSEADIQIDFTKADHDDGYPFDGPGGTVAHAFFPGDSYAAGDTHFDDDESWTFRSPDSHGMDLFAVAVHEFGHAIGLAHTSAKKSIMMPYYQGPVGDPLKYNLPYEDKVHIWQLYGVRDSVSHTARPDVSRTDDPPVLLDLPENRSTVPPAQDAPDRCNTHFDAVAQIRGEAFFFKGKYFWRLTREKHLVSLRPAQIHRFWRGLPLNLDSVDAVYERPSDHKIVFFKGEKYWVFKDNNVEEGYPRLTSDFGLPLEGVDAAFVWPHNDKTYFFMDRRYWRYDDHLRRMDLGYPKDTMLWKGVPTLLDDAMRWSDGSSYFFKGKEYWRVQGSDMEVEVGYPRSIGKDWLACREMQSDSPAADGNGTETRSQGQHHVSHAENGYEVCSCTSAATPRGPLSRTRVGRLLPLWPWTLAVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHB8</accession>
    <name>W5MHB8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="4">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diphthamide biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphtheria toxin resistance protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01005931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005934">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005940">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHB8"/>
    <dbReference type="FunCoup" id="W5MHB8">
        <property type="interactions" value="1209"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007777"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007786.1">
        <property type="protein sequence ID" value="ENSLOCP00000007777.1"/>
        <property type="gene ID" value="ENSLOCG00000006434.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2648">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153694"/>
    <dbReference type="HOGENOM" id="CLU_037146_1_1_1"/>
    <dbReference type="InParanoid" id="W5MHB8"/>
    <dbReference type="OMA" id="PGQVLGC"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006434">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="404"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="446" mass="49901" checksum="39AEDBB43F1776B6" modified="2014-04-16" version="1">RCLSVTHMCLFGKMAAAEVESRVAAIKKTAARGPRRVANQIPDEILKDPELQEAVRALPENYNFEIHKTVWRVKQVKAKRVALQMPEGLQMFACTIADIIERFTGAETLVMGDVTYGACCVDDFTARALGADFMVHYGHSCLVPIDSTAGIKMLYVFVDIQIDTPHFLDTVRFNFPRGSSLALVSTIQFVAALQSASSELRPDYDVVVPQCRPLSPGEILGCTSPRLERPVSAILEIYLIAGKYQDRHLENPSITHGVPCIVRYDPYSKVFSREHYDHDAMRAVRRQAIEAARRARRWGLILGTLGRQGNPKILEHLESRLQALGLPFVRVLLSEIFPSKLDLLADIVKWVQIACPRLSIDWGSAFSRPLLSPYEAAVALQEVEWQQTYPMDFYSNHSLGPWAVNHPANRPPRPALKPAQVRESSRGKQADSCKDAWACLKGSRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHQ9</accession>
    <name>W5MHQ9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 15">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix. Plays a key role for peroxisome movement through a direct interaction with tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with PEX13; forming the PEX13-PEX14 docking complex. Interacts with PEX5 (via WxxxF/Y motifs). Interacts with PEX19. Interacts with tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009820">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192695.1">
        <property type="nucleotide sequence ID" value="XM_015337209.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHQ9"/>
    <dbReference type="FunCoup" id="W5MHQ9">
        <property type="interactions" value="467"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007918"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007928.1">
        <property type="protein sequence ID" value="ENSLOCP00000007918.1"/>
        <property type="gene ID" value="ENSLOCG00000006553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693462"/>
    <dbReference type="CTD" id="5195"/>
    <dbReference type="eggNOG" id="KOG2629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015047"/>
    <dbReference type="HOGENOM" id="CLU_065928_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHQ9"/>
    <dbReference type="OMA" id="YNQWQPP"/>
    <dbReference type="OrthoDB" id="441517at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006553">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000296">
        <property type="entry name" value="Peroxisomal membrane protein PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="19">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="250"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="251"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="379" mass="41249" checksum="A3D08218ED81594A" modified="2014-04-16" version="1">MASSEQADQQSQTGSSQVKEDALPREQLIATAIKFLQNQKVRQSPLATRRAFLKKKGLTDEEVDLALQRSGSTEEPLPVAPASPQHLLHPPHLAPVQYNAASSRWRDYGALAIIMAGMAFGFHQLYQKYILPLIMGSKEDKKHLQRIETSISEMSGTLTQTVTQLQITLASVQELLVQQQQKIQELSQELAASEQASSAADRMLESQSINELKAEIVSLKGLLLSRRQFPASPSAPKIPSWQIPLKPATLSSSPSVNHNNSSSDISPVSNESASSSPVKEGHSPAGAPAGGQALAGEGPLAGQLPLDVKGQVRMEVQGEEERKEEEEEDDEDDDDVSHVDEEEPLPVQTEDRRGGDGQINEHVDKLRRPEGASNESEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHZ0</accession>
    <name>W5MHZ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:ch211-214c7.5</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01007826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHZ0"/>
    <dbReference type="FunCoup" id="W5MHZ0">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000008009.1">
        <property type="protein sequence ID" value="ENSLOCP00000007999.1"/>
        <property type="gene ID" value="ENSLOCG00000006625.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R1CJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009916"/>
    <dbReference type="HOGENOM" id="CLU_069377_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHZ0"/>
    <dbReference type="OMA" id="WHLETIP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006625">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021748">
        <property type="entry name" value="DUF3314"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36292">
        <property type="entry name" value="UPF0575 PROTEIN C19ORF67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36292:SF1">
        <property type="entry name" value="UPF0575 PROTEIN C19ORF67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11771">
        <property type="entry name" value="DUF3314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="338" mass="37793" checksum="7F6C5FDFAA493B38" modified="2014-04-16" version="1">MTSVKAQSQGNEPGDFPEDDPRQETLFLGTSQGETLEQPAAWDELPGSHPYTYCRVSTVDEKLWPIEHHLQCLLKKVDEFQMHLLYSRDRMPKEGFTEAVLTFFRSCQPYFAYLESTARSVAPSHRPLPQPVRTRLLQFSHLLCSRLEQLVLMYASLGLLSLEETDPCSLSQFHCGEVQLGPGCRVSAFRYCRPSPFHAGGAPGPRSRLYKCVRWNVERAPGRSWREAAGGWAEGAMETYFLCCELPAGGLSKTESGGGVASSPLRPPCAWSIGQWVQIDPDPERSDIYDWVLCVVPRGEYRPLLFLGEEEPTVSTATDYLLGLLLRQGLGKPGTEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJB2</accession>
    <name>W5MJB2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BUTYPHLNCDUF"/>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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        <property type="entry name" value="B302_SPRY"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="88" mass="10035" checksum="590E1244BA89F6B6" modified="2014-04-16" version="1">MIDAAVDVTLDLKTAHPLLKLTPDGKKVRDALDPDPARPDSPRRFRHHTCVLGCEGFSSGRQYWEVCLEQKQSWWVGVMAESAWEKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKQ5</accession>
    <name>W5MKQ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein 687b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000008964.1"/>
        <property type="gene ID" value="ENSLOCG00000007387.1"/>
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    </dbReference>
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        <property type="component" value="Linkage group LG24"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 687 (Predicted)"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Zn532-like"/>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222">
        <property type="entry name" value="ZINC FINGER PROTEIN 532-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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    <dbReference type="Pfam" id="PF25412">
        <property type="entry name" value="zf-C2H2_ZNF592"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
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            <begin position="729"/>
            <end position="756"/>
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    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="901"/>
            <end position="929"/>
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    </feature>
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            <begin position="23"/>
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            <begin position="786"/>
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    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
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        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="201"/>
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            <begin position="227"/>
            <end position="253"/>
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    </feature>
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            <begin position="287"/>
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            <begin position="363"/>
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        </location>
    </feature>
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        <location>
            <begin position="792"/>
            <end position="806"/>
        </location>
    </feature>
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        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="981" mass="104973" checksum="03C02A7F06CE60BE" modified="2014-04-16" version="1">MGDMKTPDFDDLLAAFDIPDIDAKEAIQSAPDESEGHHGNHGSIVKQGGGVGGGGGNVQLPAPQPDPSVVSVIVKNRPSLSPPPPPSSSSSSSPFNGTQVQRGPAQGGASQFRHLDSDDSDTEPDLGSPLVIQESPDSPGLLPARPSPAASPRAPLPRSPPGSPRSKSPLLTPRSPEPGPSEALGSRDKNTEHVIEERDSPESPDAEIPNSGSAGATDSKPRKAMGSEEEEEEEEEEEEEQEGMEVEDGEAEGESVREKEPPGGEGLSPIGRSLTRPAPSSVPPPGTAAAAAASRPLKVRIKTVKTETGVITRTVTRVAPAAGGRGADPAKAGAAQRKASGQQPPGTGRRTAASGKSRKTTDALSARSANQQQQLQQRAKGPVFPVSTLQDASTAMLVAASKAQTKMAAAAQSSSSAEKTRTPASVAAAAITITKATALPIASSSPSSSSSAPAKFGVSGGITVRSVTQKTVNGTQVVMASKPASIVNSSGAVISRSQSSLVEAFNKILNSKNLLPSYRPDLSTPPPPEWSLPLPANGYRCLECGDAFALERSLARHYDRRSLRIEVTCNHCAKRLAFFNKCSLLLHAREHKERGLVMQCSHLVMRPVTVEQMIGQQDTVPIGEGGGCSRPVCLRGSPVSDLCICTCLSVPVCISVVLYLLMRHSLYLLTCLSVSTTLLRLIWFNCHSFSLCVQSLSGLPPYCALENCDEVIAGAAYPRSMPLSPRMIFKCVMCDTVFTQKPLLYMHFDTHLAKQKVHVFKCPDCTKLYAQKGSMMEHIKTVHRGLSVKQEPPSPSPGLAPPPASAPKPSRSMKSEPRQSSSDGEEGTERGREEEEEEEGEGAEDEEEEEPPSSPEARASRPPRRPLPSEWSCRQCQTRYTERDEYIAHMRKEHGKSVKKFPCRLCERSFCSAPSLRRHVRVNHEGIKRVFHCQYCTEGKRTFSSRLILEKHIRVRHGIRTRDQSAGRQPGDPEGGAPASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKW2</accession>
    <name>W5MKW2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-secretase subunit APH-1</fullName>
            <shortName evidence="7">APH-1</shortName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Potential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the gamma-secretase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the APH-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626961.2">
        <property type="nucleotide sequence ID" value="XM_006626898.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKW2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009021"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009032.1">
        <property type="protein sequence ID" value="ENSLOCP00000009021.1"/>
        <property type="gene ID" value="ENSLOCG00000007436.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689245"/>
    <dbReference type="KEGG" id="loc:102689245"/>
    <dbReference type="eggNOG" id="KOG3972">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002049"/>
    <dbReference type="HOGENOM" id="CLU_086389_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKW2"/>
    <dbReference type="OMA" id="LVWFIAM"/>
    <dbReference type="OrthoDB" id="6507463at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007436">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007220">
        <property type="term" value="P:Notch receptor processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009294">
        <property type="entry name" value="Aph-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12889">
        <property type="entry name" value="GAMMA-SECRETASE SUBUNIT APH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06105">
        <property type="entry name" value="Aph-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009021.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="256" mass="28264" checksum="A1905BC931ED6A29" modified="2014-04-16" version="1">MTVAVFFGCTFIAFGPAFALFIFTIARDPLRVIILIAGAFFWLLSLLLSSLVWFIAMKATGGQDYQLQRGLLIFGVMFSVILQEVFRFAYYKLLRKANEGLAALSEDEHSPISVQQMAYVAGLGFGIMSGAFSMINILSDSLGPGTVGIHGDSQYYFITSAFMTLAVTLLHTFWGVLFFDGCERRRWWVIALVVALHLLVSGLTFLNPFYEGSLPPAYIIMVVMGIWAFFSSGGSLQNILHVLSCRKNGVDSQVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMC0</accession>
    <name>W5MMC0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Solute carrier family 12 member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Na-Cl symporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Thiazide-sensitive sodium-chloride cotransporter</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>chloride(out) + Na(+)(out) = chloride(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73887"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; adopts a domain-swap conformation at the scissor helices connecting the transmembrane domain and C-terminal domain. Interacts with KLHL3. Interacts with IL18R1; this interaction is increased by IL18 treatment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020807">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMC0"/>
    <dbReference type="FunCoup" id="W5MMC0">
        <property type="interactions" value="607"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009529"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009540.1">
        <property type="protein sequence ID" value="ENSLOCP00000009529.1"/>
        <property type="gene ID" value="ENSLOCG00000007841.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2083">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155044"/>
    <dbReference type="HOGENOM" id="CLU_001883_0_0_1"/>
    <dbReference type="InParanoid" id="W5MMC0"/>
    <dbReference type="OMA" id="PWMITEQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007841">
        <property type="expression patterns" value="Expressed in mesonephros and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035844">
        <property type="term" value="P:cloaca development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:potassium ion homeostasis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039023">
        <property type="term" value="P:pronephric duct morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:sodium ion homeostasis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="solute carrier family 12 member 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013612">
        <property type="entry name" value="AA_permease_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002948">
        <property type="entry name" value="SLC12A3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF9">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08403">
        <property type="entry name" value="AA_permease_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01230">
        <property type="entry name" value="NACLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0868">Chloride</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="568"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease N-terminal" evidence="29">
        <location>
            <begin position="50"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="27">
        <location>
            <begin position="140"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="28">
        <location>
            <begin position="651"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="28">
        <location>
            <begin position="818"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00056815"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063035"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00073714"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF08403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1028" mass="113129" checksum="8D0EB07865D2BF08" modified="2014-04-16" version="1">MADVPAPVVTVDSPPPHGRFTISRAVSSASREEDSKFSYEQPESGVNSMDSETSSLSLRTSAYNTLDVAPSYDFYANTEIPGRDRRQRPSLFQLHSYTEEGSCPPPVYEDASGAGRSAWQDTAARRVAPSRAGWSLWQHIRCMLNIWGVILYLRLPWITSQAGIGLTWVIILLSSCITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVQVLIQESGSSIVDPINDIRIVGVITVTCLLGISLAGMEWESKAQVLFFFVIMVSFANYIVGTIIPATPEKKAKGFFSYKGAIFAENFVPNWRGPEGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPRGTLLAIFWTTMSYLIISATIGSCVVRDASGNINDTADALGTDCLGLACTYGWNFSDCIANKTCPYGISNYYQSMSMVSGFAPLITAGIFGATLSSALACLVSAPKVFQCLCKDNLYPLIGFFGEGYGKNNEPIRGYLLTYIIAICFILIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYSKWAALVGAIISVVIMFLLTWWAALIAIGIVLFLLAYVLYKRPAVNWGSSVQAGSYNMALSYSVGLNEVDEHIKNYRPQCLVLTGPPSFRPALVDFVGTFTKNLSLMMCGNVVVGAPSPTSQEAAGSGSHVTWLNRRKIKSFYRGVVAEDLRSGVQMLLQAAGLGRIQPNVLVMGFKRNWQRDRPSSLENYIGIIHDAFDLRFGVCVMRTKEGLSRTMQAHVNPVFENGSEYRGNGTSPAPAAHSTLDPDALVAEVQPTTIFQSKQGKKTIDVYWLFDDGGLTLLIPYLLTRKKRWRGCKVRVFVGGEISRVDEQRKAFMSLLSKFRLGFHDVQVLPDVNEKPRPEHVKRFEDLLGPYRLNDGFRDENTVNEMKKEFPWKISDEEIERNKAKSLRQIHLNEILLDYSRDAALIILKCPLVPVGDQSELQISRHLFQMAWLETLSQDLRPPVLLVRGNQENVLTFYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMR7</accession>
    <name>W5MMR7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cathepsin K</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028263">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028264">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMR7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009676"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009687.1">
        <property type="protein sequence ID" value="ENSLOCP00000009676.1"/>
        <property type="gene ID" value="ENSLOCG00000007964.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157759"/>
    <dbReference type="HOGENOM" id="CLU_012184_1_2_1"/>
    <dbReference type="InParanoid" id="W5MMR7"/>
    <dbReference type="OMA" id="GHAYWIV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007964">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000006">
        <property type="entry name" value="Cathepsin S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018627567" evidence="5">
        <location>
            <begin position="23"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="6">
        <location>
            <begin position="124"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="341" mass="38053" checksum="A828FB39E88774F1" modified="2014-04-16" version="1">DNDEQTMLLCGGVLLLMASALAYPSQQEWLDSEWESWKMTYRRQYNGLVLPRQGPNGSWLERSVALIQWLSSQVCARVRGKHIYSTRNNRDETSEEVAEKMMGLQVPMFRNSSDTYIPDDMQKLPKAIDYRKLGYVTPVRNQGSCGSCWAFSSAGALEGQLKKTTGQLVSLSPQNLVDCVKENDGCGGGYMTNAFKYVMTNRGIDSEESYPYVGEDQQCAYNKTGRATGCKGYKVIPEGSEKALQAAVAKIGPVSVGIDATLYSFQFYKRGVYYDRNCNKDDINHAVLAVGYGATPKGKKYWIIKNSRSWGEDWGHKGYILMARNRNNFCGIASLASVPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN45</accession>
    <name>W5MN45_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase like 25B</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN45"/>
    <dbReference type="FunCoup" id="W5MN45">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009804"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009815.1">
        <property type="protein sequence ID" value="ENSLOCP00000009804.1"/>
        <property type="gene ID" value="ENSLOCG00000008069.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063745"/>
    <dbReference type="HOGENOM" id="CLU_016581_3_0_1"/>
    <dbReference type="InParanoid" id="W5MN45"/>
    <dbReference type="OMA" id="IVGLHPC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008069">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF2">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 25B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="141"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="656" mass="71046" checksum="3F1180B2E4B0999A" modified="2014-04-16" version="1">MTAVGVSISSQTFSAEQQKHLAASITSFLSRYKHITDSYIIEFFSEGLWDTLPPAWQEVLCSLSAPQIADLLLDSTPQGGRSYASVWPLSLLAFRETAHALAFPRMPCRQAAGCGRPAEFHQNPCQSSLLTHIFRKHVKPKKQHEIRRLGALVKKLSDLTDCRKVVDVGSGQGHLTRFLSFGLGLSVIGMEADPALVSMAAKFDRQLLCTLQKETQRKNGGAHVSLLPTGPLPSHVTGWVNPRASWEEFTKLLQRAEGQGVQAGTPGNRKRREDGLPHADQEPLPVCQPLCPETPPGCQNRAWGEGNCTQNQAREDAEFCPSPKPSRTNCNPDQNPSQLTASPEQTCPARFPCWASSKSPCLDHTGAPVAENGPEAGGFILTGLHACGDLSSALLRHFANCPDVVGITSVACCYMKLSTADNPTPPGVLPPPSPTHGGGATLKGEGPSCGYPMSCYVKALPGHQLSYKAREGACHALEDYAQRLRSESGLLKTHCYRAVLETVIRRVEPRLRRPGIQTIKKAHELPFAEYARLGLRRVGLPADPPLDSASLDAMLSQQGKVVAYFSLALLLAPVVETLVLLDRILYLQERGLKSQLTPLFDPHFSPRNLVLLAVKPAGSGGHRARRDGRDVPRGAAVCHPGAVLGTRPQEGSRLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNT3</accession>
    <name>W5MNT3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNT3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010054.1">
        <property type="protein sequence ID" value="ENSLOCP00000010042.1"/>
        <property type="gene ID" value="ENSLOCG00000008264.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1966">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159190"/>
    <dbReference type="HOGENOM" id="CLU_005912_4_2_1"/>
    <dbReference type="InParanoid" id="W5MNT3"/>
    <dbReference type="OMA" id="AMHHLLC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008264">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018410">
        <property type="entry name" value="Na/H_exchanger_3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF56">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01087">
        <property type="entry name" value="NAHEXCHNGR3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868484" description="Sodium/hydrogen exchanger" evidence="16">
        <location>
            <begin position="19"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="17">
        <location>
            <begin position="99"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="693"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="857"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="892"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="941"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="695"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="941"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="960" mass="106659" checksum="036DFB6BF0233A6D" modified="2014-04-16" version="1">MLSCAAYLLVTAATETLSDLYVAEASVIPEPAYGLSSVSTPIPIGDPANTGATAVLPDGKTAEPCDTGAGSHTATMTEFEIVKWEWSYVQAPYLTVIWIMIASFAKILFHLSHSVTRVVPESCLLILLGLGLGGIVLAATKKPEYQLHPELFFLFLLPPIVLDSGYFMPSRLFFDNIGTILMYAVIGTLWNSFSTGLCLWGVKGAGLMDCNVNAGLMDFLLFGSLISAVDPVAVLAVFEEVHVNETLFIIVFGESLLNDAVTVVLYKVFNSFVEMGTDRVRPVDYVKGVASFFVVSLGGTVIGLIFAFVLALITRFTKRVRIIEPLFVFLLVYLAYLTAEMVSLSSILAMTFCGICCKKYVEANISQKSRTTVKYTMKTLASIAETIIFIFLGISAVDSSKWAWDTGLVLSTLFFIFVLRAIGVVFQTWVLNQFRLSPLDKIDQVVMSYGGLRGAVAFALVILLNETKVKAKDYFVATTIIVVFFTVIIQGLTIKPLVKWLKVKRSSQHKPTLNEEIHDRAFDHILAAVEDISGLHGYHHWRDKWEHFDKQYLSQVLMRKSAYRIKDDIWDMYQKLNVRDAISFVDQGGHVLSSAKLVLPSMPSRTSFSENSVTNLLRENGSGVCLDLQVIDTVRSVRDREDTVMHHVLTGNLYKPRRRYQANYSRHFMSAGEQERQDHEVFQRNMRRRLESFKSTKHHSYQGKAKSRYKKEHHAQKKPKCTDVGELVPNGKPRRNVSWQDTVPVVVTVDSEDDKSETEKEDEGITFVARTADEVLQEPLPVLPPGGQDVCHSPFTIPPSPTCAERQLPWKGDQGSLPVCVSLETTKIVPIDLQQAWNQSISSLESLASPPVAAESQSHCRAPLCRQEQRGSPSARGARGQGFLFQFPDWAAGQSGEEDSSQPVQQQEMQPLMSTGRAPKPVSPPSCVTGRWPPRISLLKSLVSVPPSASEPRSRKPTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP41</accession>
    <name>W5MP41_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020072">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MP41">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010150"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010162.1">
        <property type="protein sequence ID" value="ENSLOCP00000010150.1"/>
        <property type="gene ID" value="ENSLOCG00000008347.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163423"/>
    <dbReference type="HOGENOM" id="CLU_000540_5_0_1"/>
    <dbReference type="InParanoid" id="W5MP41"/>
    <dbReference type="OMA" id="ISVIPXH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008347">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF278">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 22" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 22" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 22" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 22" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 22" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="728"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="805"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="847"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="937"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1186"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1225"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1256"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1302"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1421"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1509"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1539"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1569"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1626"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1730"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="126"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="27">
        <location>
            <begin position="536"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="736"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium ion transport-associated" evidence="26">
        <location>
            <begin position="974"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1184"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1508"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="28">
        <location>
            <begin position="1875"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="452"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1098"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1932"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="496"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1941"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1955"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023053"/>
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            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038083"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055248"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF24609"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1981" mass="225400" checksum="9F5C6312D3E10AB9" modified="2014-04-16" version="1">MAQLLVPPGPDSFHLFNQESLQAIEKRIAEEKGKKPKGERPDDDENGPKPNSDLEAGRSLPFIYGDTPKGMVSEPLEDLDPYYINEKTFIVLNRGKTIFRFNATPALYILSPFNPLRKIAIKILVHSLFSMLIMCTILTNCSFMTLSNPPDWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRHKCIKWPIDNVSSINTSDNSFNFLLLFSLDNYYFLPGRRDALLCGNGSDAGQCPEGYTCIKGGQNPDYGYTSFDTFSWAFLSLFRLMTQDFWENLYQQTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAYEEQNQATMEEAEQKEAEFQAMLEQLKKQQEGAQVSAAAAAAAESGEYSGGGGLTETSSEASKLSSKSAKERRNRRKKRRQKDEEEEKGDNEKFHKSESEDSITKRRVFGISIDGNRLSYEKRYSSPHQSLLSMRGSLFSPRRNSRASLFSFRGRARDVGSENDFADDEHSTFEDNDSRRGSLFVPRRQERRNSNISQNSRSSRVLLPANGKMHCTVDCNGVVSLVGGPSVPTSPVGLLLPEATTTTETEIKKKRSGSHQASMDYLEDPSIRQRALSVASILTNTMEELEESRQKCPPCWYKFANTYLIWDCCPQWLKIKEIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTKEFNHVLSVGNLVFTGIFTAEMFFKVIAMDPYYYFQEGWNIFDGFIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYRDCVCKISEDCTLPRWHMFDFFHSFLIVFRVLCGEWIETMWDCMEVAGQSMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDSEMNNLQIAVGRIHRGVDYIKGNLRESVQKLCVRRKKVLDEIKPLEDLNNKKEACISNHTTVELTKEPEYHKDGNGTTSGIGSSVGKYVIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEDFSSESDIEGSKEKLDQSSSSEGSTVDIRPPGEGEPIEIEPEESFEPEACFTEGCVRKFQFCQVNIEEGYWKQWWTLRKTCFRIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFVKYFTNAWCWLDFLIVDVSLVSLVANALGYSELTAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYHCVNTTTDERFLVNVVNNMSDCLKLGNGSRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDLEDQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKVHYFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGCVFDVVTKQAFDITIMILICLNMVTMMVETDDQSKDMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSRAPDCDSEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEYAKLSDFADALEPPLRVPKPNKIQLIAMDLPMVSGERIHCLDILFAFTKRVLGEGGEMDALRGQMEERFMASNPSKVSYEPITTTLRRKQEDVSAVIIQRAFRRYLMRRTVKQASFMYKEQNKVGVHILDKEEMVIDKYNENSTTEKTDMTPSTASPPSYDSVTKPDKDKYEKDKREKEDKGKDVREHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ36</accession>
    <name>W5MQ36_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01003930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQ36"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010495"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010510.1">
        <property type="protein sequence ID" value="ENSLOCP00000010495.1"/>
        <property type="gene ID" value="ENSLOCG00000008632.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154598"/>
    <dbReference type="HOGENOM" id="CLU_011511_1_2_1"/>
    <dbReference type="InParanoid" id="W5MQ36"/>
    <dbReference type="OMA" id="SKANWSA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008632">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF9">
        <property type="entry name" value="POLY(A) POLYMERASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="19">
        <location>
            <begin position="20"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="18">
        <location>
            <begin position="218"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="17">
        <location>
            <begin position="370"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="503"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="578"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="630"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="671"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="718" mass="79794" checksum="67C2B7A7E744CA02" modified="2014-04-16" version="1">CSPVANQAVQALQQPTKHYGITSPISLALPKEADLVLTQKLTEALKPYGVFEEELELQRRILVLGKLNTLVKEWIRDISETKNLPASIIDNVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERSDFFTSFYEKLKEQEEVKDLRAVEEAFVPVIKLSFDGIEIDILFARLALQTIPEDLDLRDDGLLKNLDIRCIRSLNGCRVTDEILHLVPSIENFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAVASTLVHKFFLVFSKWEWPNPVLLKQPEDCNLNLPVWDPRVIIYPVFHKLQVCLYPHCFVNFMYHIFLNNVRPFILHFSCVLAPCNNMSYTVFFHEKCAYILIFKIPRHYIVLLASAPTEKQHLEWVGLVESKIRILVGNLEKNEFITLAHVNPQSFPGPKEGSEKEEFSTMWVIGIAFKKMEGAENLNVDLTYDIQSFTDTVYRQAISSKMFEPDMKITAMHVKRKQLHQLLPNLVIQKRRKHSTEGVKPVNESSLDLSLDSDNSMSVPSPTTAFSSSLRRLSPAAPVANSQASEGPCRGEGAGSSVSPGVPSDGTPPKPVAVAPPPKPAAARGVSSTPVIKRPLLAAGGSAAKPAAKRTGSPIQEETPKKPRAEEDPSESRVSGDNTAQPDVKPSVEMEALPGHSRALSSTAPCPQENTPIFQRIPSTDLSDVPLLPANPIPVVKNSIKLRLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQK7</accession>
    <name>W5MQK7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysyl oxidase homolog</fullName>
            <ecNumber evidence="13">1.4.3.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-lysyl-[protein] + O2 + H2O = (S)-2-amino-6-oxohexanoyl-[protein] + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24544"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="EC" id="1.4.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>lysine tyrosylquinone residue</name>
            <dbReference type="ChEBI" id="CHEBI:20489"/>
        </cofactor>
        <text evidence="13">Contains 1 lysine tyrosylquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the lysyl oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.13" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01002777">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQK7"/>
    <dbReference type="FunCoup" id="W5MQK7">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010666"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010681.1">
        <property type="protein sequence ID" value="ENSLOCP00000010666.1"/>
        <property type="gene ID" value="ENSLOCG00000008751.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154779"/>
    <dbReference type="InParanoid" id="W5MQK7"/>
    <dbReference type="OMA" id="GCHMSTY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008751">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035791">
        <property type="term" value="P:platelet-derived growth factor receptor-beta signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019828">
        <property type="entry name" value="Lysyl_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF6">
        <property type="entry name" value="PROTEIN-LYSINE 6-OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00926">
        <property type="entry name" value="LYSYL_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 13" id="KW-0886">LTQ</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="8 13" id="KW-0801">TPQ</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="90"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="445" mass="50985" checksum="8054E1A59C10BCE1" modified="2014-04-16" version="1">YKMKTRGFDAVIYLCIPLFYLSCVLHIVECQQQQNTRPGNPPPAGLFRQKIQWENNGRVFSLLSHGAEYQPPRRRDHNTKPEEPKHIIIISNNQTTEVQNSRPASVQQNSQSTTAAQSRPGAGPRSGYWFQAGVNRRGRPTGASSATQERQARPGPNSAGARQSTAETTSGTRNENRTGQNPPPLSNLRPEDGMVGDDPYNPYKSTDDNPYYNYYDTYERQRPNGGQRPGYGTRYFQYGLPDLVPDPYYIQASTYVQRMAMYNLRCAAEENCLASSAYRSEVRDYDTRMLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLEANSQRRVAEGHKASFCLEDSSCDYGYYRRYACTSHTQGLSPGCYDTYNADIDCQWIDITDVKPGNYILKVSVNPSYQVPESDYTNNIVRCDIRYTGHYVYTSGCRLSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MR64</accession>
    <name>W5MR64_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine dioxygenase</fullName>
            <ecNumber evidence="13">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of cysteine to cysteine sulfinic acid with addition of molecular dioxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01002843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626919.1">
        <property type="nucleotide sequence ID" value="XM_006626856.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MR64"/>
    <dbReference type="FunCoup" id="W5MR64">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010873"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010889.1">
        <property type="protein sequence ID" value="ENSLOCP00000010873.1"/>
        <property type="gene ID" value="ENSLOCG00000008927.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698527"/>
    <dbReference type="KEGG" id="loc:102698527"/>
    <dbReference type="CTD" id="1036"/>
    <dbReference type="eggNOG" id="KOG4064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018226"/>
    <dbReference type="HOGENOM" id="CLU_079443_1_0_1"/>
    <dbReference type="InParanoid" id="W5MR64"/>
    <dbReference type="OMA" id="YTENQVT"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="UniPathway" id="UPA00012">
        <property type="reaction ID" value="UER00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008927">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000045">
        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="11">
        <location>
            <begin position="93"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="201" mass="23379" checksum="4EBA3E91B3BA7DB0" modified="2014-04-16" version="1">MEQTEVMKPETLDDLIKILHEIFSGNNINIEEVQSIMESYESKPQEWKKYAKFDQFRYTRNLVDEGNGKFNLMILCWGEGHGSSIHDHTDSHCFMKLLQGQLKETLFDWPEKKAHGAMKKKSERVLPENQCAYINDSIGLHRVENVSHTECAVSLHLYSPPFDYCQTFDQRTGHKNPVKMTFWSKFGEKTTNETAVSQENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS78</accession>
    <name>W5MS78_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytidine and dCMP deaminase domain-containing protein 1</fullName>
            <ecNumber evidence="3">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine deaminase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxycytidine + H2O + H(+) = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01003453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219929.1">
        <property type="nucleotide sequence ID" value="XM_015364443.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069034879.1">
        <property type="nucleotide sequence ID" value="XM_069178778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS78"/>
    <dbReference type="FunCoup" id="W5MS78">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011237"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011253.1">
        <property type="protein sequence ID" value="ENSLOCP00000011237.1"/>
        <property type="gene ID" value="ENSLOCG00000009213.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684421"/>
    <dbReference type="KEGG" id="loc:102684421"/>
    <dbReference type="CTD" id="81602"/>
    <dbReference type="eggNOG" id="KOG3127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153676"/>
    <dbReference type="HOGENOM" id="CLU_038832_1_0_1"/>
    <dbReference type="InParanoid" id="W5MS78"/>
    <dbReference type="OMA" id="ITHIYTT"/>
    <dbReference type="OrthoDB" id="6710946at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009213">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000028">
        <property type="entry name" value="Cytidine and dCMP deaminase domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000030">
        <property type="entry name" value="Cytidine and dCMP deaminase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF14">
        <property type="entry name" value="CYTIDINE AND DCMP DEAMINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="12">
        <location>
            <begin position="53"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="12">
        <location>
            <begin position="305"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="504" mass="56905" checksum="302D0D8B97412509" modified="2014-04-16" version="1">MAASAGFPVKMGSEENRAGCSCKGHVSVSEASTQTDTKVQGHIPRLSKANLFTLLSLWMELYPKNKKDQENGTQQIRTTGLVVVNNRQITGLHCSSTELHAGQIAVIKHGPRLKDCDLYFSRKPCCTCLKMIINAGVNRIVYWPADPEVSLLGSKHRSWDAALDASAAERLKSNSRPHICVLLQPLASNMHQYVEETSKNCDFLGEIACENPKLNVEELFAEHRRQKLADYLKVFFVPDEAQHKEILKIMGLQNLCCEPYYSSLRQHMRDLLLVLAAVDSSVPQGHGYGFYCRDVQEAGEGLSQDIARHCVVQARLLAYRTEDPKVGVGAVIWAEGKSSNCDGTGRMYLVGCGYNAFPVGSEYAEFPQMDNKQRDRETRKYRYIIHAEQNALLFRSKEIKEEENTMIFITKCPCDECVPLIKGSGIKQIYTVDRDSGKDKDDISYLRFDSLQGLQKFKWQRNIEDTKSLEQNPTAIRTNGFLGKHGRQVEEEHWTKKKACLQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSU3</accession>
    <name>W5MSU3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thrombospondin type laminin G domain and EAR repeats</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Stereocilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSU3"/>
    <dbReference type="FunCoup" id="W5MSU3">
        <property type="interactions" value="1361"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011452"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011469.1">
        <property type="protein sequence ID" value="ENSLOCP00000011452.1"/>
        <property type="gene ID" value="ENSLOCG00000009387.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047718"/>
    <dbReference type="HOGENOM" id="CLU_028578_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSU3"/>
    <dbReference type="OMA" id="THGARDW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009387">
        <property type="expression patterns" value="Expressed in zone of skin and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009039">
        <property type="entry name" value="EAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005492">
        <property type="entry name" value="EPTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15261">
        <property type="entry name" value="THROMBOSPONDIN-TYPE LAMININ G DOMAIN AND EAR REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15261:SF4">
        <property type="entry name" value="THROMBOSPONDIN-TYPE LAMININ G DOMAIN AND EAR REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03736">
        <property type="entry name" value="EPTP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50912">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="4">
        <location>
            <begin position="39"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="652" mass="74677" checksum="7B97641D6D89828A" modified="2014-04-16" version="1">MRSGLAGGWKPCTDLLPLDFLSRVLPAGGMALSGVRMVQDQGARGLQFMAPHQALSFPASQLFINCDYFPEEFSIVTTLKISRIASKKNEYLFSVVEEDSNSLLLGLRYSQDKLHFLFSNYRKRKRISFRSMRLADNQWHTLILAVSGYYASLTVDCGMPIDLFFDKPFPSSLSTKGSRFYIGNRRRWKGLFSGLLRQLVLLPGSDATSRICPSLHPQLAILSVPQALLDLPAKPSGNEVLKYPYEAELRVTVGSRPPCTKMEMGQLWFDTLKRGLYTCDSRDWIAMFQEKERLDYVEDYQDLYTNSETLDIEVFEIPSVGLFVATANRKHYPGSGIYKWTNGKFEPYQYITTYEAQAWKHFTVDDKVFLVIANFKKNERNQEHSVIYIWSQRKLKFIVYQTLETHGARDWEAFHIEGETFLAVANHIQGDNHNIQSVIYKWNPATQLFEANQTISTSGAYDWEFFTVGPFSFLAVANTFNGISTHIDSKIYVWFGGKFQHFQSITTDGATDWEVFKIGDRIFLAVANSQKYDEAGPGSYAINSTIYELNMTAQLFVKFQDITTYRISAVDWEFFTLGEDKFLVVANSFDGTSFSVNSVIYRWQGYEGFVPVHRLPTFGCKDWEYFSSTEGSYLIYSSAKEPRSKVLKLKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUQ7</accession>
    <name>W5MUQ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">The ATPase pump is composed of two subunits: alpha (catalytic) and beta (regulatory). Interacts with alpha subunit ATP12A; this interaction is required for the formation of a functionally active pump and targeting at the plasma membrane. Interacts (via N-terminus) with alpha subunit ATP4A (via the P-domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003530">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUQ7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012137.1">
        <property type="protein sequence ID" value="ENSLOCP00000012116.1"/>
        <property type="gene ID" value="ENSLOCG00000009911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009911">
        <property type="expression patterns" value="Expressed in larva and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000061">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000006">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF11">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="33409" checksum="51688A1076909B6A" modified="2014-04-16" version="1">MATLKEKRTCGQRCEDFGHFIWNSENKTFMGRTPLRWVYISLYYVAFYIVMTALFSLAIYVMMSTMNPYTPTYQDRLKSPGVMIWPDVYGDEDLEIYYNMSDKSSWTKMAEILKEFLKPYNETVQYHQSKQCEKGKYNKQDTFGPHHTKTSCVFTQEMLGNCSGISDPTFGYPDGKPCFIIKMNRIINFLPGEGVAPYVNCTVLEGSPSNLDGVEYFPTNGTFDLSYFPYYGKLAQPHYSNPLIAVKFTSMERNKVVKLECRVVGKGISSQHCSQCYHFAITFHFFPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV34</accession>
    <name>W5MV34_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">EGF-like-domain, multiple 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nephronectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV34"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012243"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012264.1">
        <property type="protein sequence ID" value="ENSLOCP00000012243.1"/>
        <property type="gene ID" value="ENSLOCG00000010006.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000150973"/>
    <dbReference type="HOGENOM" id="CLU_036867_0_0_1"/>
    <dbReference type="InParanoid" id="W5MV34"/>
    <dbReference type="OMA" id="NGRACID"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010006">
        <property type="expression patterns" value="Expressed in larva and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070371">
        <property type="term" value="P:ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903672">
        <property type="term" value="P:positive regulation of sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905564">
        <property type="term" value="P:positive regulation of vascular endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000187">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000476">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF24">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866717" evidence="15">
        <location>
            <begin position="22"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="220"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="17">
        <location>
            <begin position="413"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="564" mass="62985" checksum="24368EF1194ECCB4" modified="2014-04-16" version="1">MMPITWISVLSFFLYISTGNADIYRMLRHNRQVSSGTQPGVCRYGRRLECCYGWKKNDKGQCEALCEHGCKHGECVGPNKCKCFPGYTGKACNQDLNECGLKPRPCEHRCMNTHGSYMCYCLNGYMLMADGSCANSRTCIMAHCQYGCEEVQGEVRCLCPSAGLQLGPDERTCVDVDECSTGKRLCPYNRRCVNTFGSYYCKCQIGFDLEYVDGKYDCVDVDECASNTHKCSPQAQCLNTHGSYKCKCKPGFRGSGFDCSVKPFFQNSWDGDKGSADDFLNAIPDSPSKESPRILGNGKDPLTKLVVDNNRELEGEQRKNVIPEPQPTAPPKNRLQPFDYEDGIYIGGSVEEKDPEDDVFLEEEDNLENQIESKETSRRGDVFIPEEYKSVIGRLTVTKEAPAVVPGHEEFIIDCSFNQGACEWMQDTEDDFDWSVADHDNGIGYYMAVPGFVGERKDVGRLKLLLSDSASQSGFCLTFNYRLIGERIGKLRILLDDSGSPLWERSKSRDESWQTELVTVSWSKAAPEHVVFEAERGKGKAGEIGLDDVVLTSGSCQEDDSVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVN6</accession>
    <name>W5MVN6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69). Interacts with CCR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVN6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012445"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012466.1">
        <property type="protein sequence ID" value="ENSLOCP00000012445.1"/>
        <property type="gene ID" value="ENSLOCG00000010172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S8M4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263482"/>
    <dbReference type="HOGENOM" id="CLU_141716_4_2_1"/>
    <dbReference type="InParanoid" id="W5MVN6"/>
    <dbReference type="OMA" id="CFEFFRN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010172">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3 9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005150871" description="C-C motif chemokine" evidence="9">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="10">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="91" mass="10401" checksum="9BE36C36BD1659F9" modified="2014-04-16" version="1">MKPSQLVLAALLLAALCAPALSQNSNLPSECCFNYYPKRIFKRLVVKYELTRKDCSKKGVIFTSMKGFRICADPRIDWVKQVISYLDEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVS2</accession>
    <name>W5MVS2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like family member 18</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVS2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012481"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012502.1">
        <property type="protein sequence ID" value="ENSLOCP00000012481.1"/>
        <property type="gene ID" value="ENSLOCG00000010189.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157026"/>
    <dbReference type="HOGENOM" id="CLU_004253_14_2_1"/>
    <dbReference type="InParanoid" id="W5MVS2"/>
    <dbReference type="OMA" id="DRWTIVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010189">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18457">
        <property type="entry name" value="BACK_KLHL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18247">
        <property type="entry name" value="BTB_POZ_KLHL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030603">
        <property type="entry name" value="KLHL18_BTB/POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF497">
        <property type="entry name" value="KELCH-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="48"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="584" mass="65019" checksum="D86CF885F25ADC32" modified="2014-04-16" version="1">FSVVFIFSMKMEDLIFEELDDLMHFSVSDLPNRGYAVMEEIRRQGKLCDVTLKVGDNKFSAHRIVLAASIPYFHAMFTNDMMECKQDEIVMQGMDPSALEALINFAYNGHVAIDQQNVQSLLIGASFLQLQNVKDACCSFLRERLHPKNCLGVRQFAETMMCTTLYDAANSFIHQHFVEVSMSEEFLALGAEEVLELVSRDELNVKEEEQVFEAVLAWVRHQREERVSQLPELLSKTRLPLCRPQFLSDRVQQDELVRCSHKCRDLVDEAKDYHLMPERRPHLPAFKTRQRCCTTIAGLIYAVGGLNSAGDSLNVVEMFDPIGNCWERCQPMTTARSRVGVAVVNGLLYAIGGYDGQSRLSTVEVYNPETDIWTQVASMNSKRSAMGTVVVDGQIYVCGGYDGKSSLNSVECYSPEADKWTMATPMNASRSAAGVTVFEGRIFVSGGHDGLQIFNSVEYYNQHTASWHPVSGMMNKRCRHGAAALGSKLYVAGGYDGSGFLSVAEVYSSIADQWSHLVAMNTRRSRVSLVANCGRLYAVGGYDGQSNLNSVEMYDPETNRWTFMAPMVCHEGGVGVGCVPLLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWN4</accession>
    <name>W5MWN4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01014321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628372.1">
        <property type="nucleotide sequence ID" value="XM_006628309.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWN4"/>
    <dbReference type="FunCoup" id="W5MWN4">
        <property type="interactions" value="564"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012793"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012818.1">
        <property type="protein sequence ID" value="ENSLOCP00000012793.1"/>
        <property type="gene ID" value="ENSLOCG00000010440.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691327"/>
    <dbReference type="KEGG" id="loc:102691327"/>
    <dbReference type="CTD" id="4893"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158947"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="W5MWN4"/>
    <dbReference type="OMA" id="RAVDIWG"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010440">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="189" mass="21471" checksum="8FB2B6088FC95501" modified="2014-04-16" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADIHLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAQELARSYGIEFVETSAKTRQGVEDAFYTLVREIRHYRMKKLNSREDRKQGCMGVSCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX59</accession>
    <name>W5MX59_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nocturnin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Carbon catabolite repression 4-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001307">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629686.2">
        <property type="nucleotide sequence ID" value="XM_006629623.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX59"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012968"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012995.1">
        <property type="protein sequence ID" value="ENSLOCP00000012968.1"/>
        <property type="gene ID" value="ENSLOCG00000010593.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682701"/>
    <dbReference type="KEGG" id="loc:102682701"/>
    <dbReference type="CTD" id="572044"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155249"/>
    <dbReference type="HOGENOM" id="CLU_016428_1_2_1"/>
    <dbReference type="InParanoid" id="W5MX59"/>
    <dbReference type="OMA" id="RAACSMG"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010593">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09096">
        <property type="entry name" value="Deadenylase_nocturnin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000012">
        <property type="entry name" value="nocturnin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034965">
        <property type="entry name" value="Deadenylase_nocturnin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="153"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="438" mass="49472" checksum="A1335DE0E51D59A9" modified="2014-04-16" version="1">MYQSSVRLCSTLLQRDIPVLRVSALGSPVKKAATLKRSSSPGPRYCHSVCADNLSSRTSVPAAPTGLVSLRAVCSMGNSTSRLYSALAQTLSSTPLSQQEQYREQPLEVEPIDPEELLKKCEEVLQNRPPRLNRSFVFPKKSRPSPHRPIRVMQWNILAQALGEGKDSFVQCPLDALKWAERKYLILEEILTYRPDIFCLQEVDHYFDTFQPIFTSLGYHSSFYPKPWSPCLDVEHNNGPDGCALFFNKERFELVDSTNMRLVAMMLKTNQVAIAQTLRCKETGMLFCVAVTHLKARSGWERFRCSQGSDLLQNLQSITKGTGIPLLICGDFNAEPTEDVYRHFANSSLNLNSACKLLSHDGQSEPPYTTWKIRPSGESCHTLDYIWYSQHAFNVDSVLDFPTEEQIGPNRLPSYNYPSDHLSLVCDFSFTGEPDRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY03</accession>
    <name>W5MY03_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BET1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Golgi vesicular membrane-trafficking protein p18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with SNARE complex members GOSR2, SEC22B and STX5. Interacts with LMAN1/ERGIC53. Interacts with STX17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY03"/>
    <dbReference type="FunCoup" id="W5MY03">
        <property type="interactions" value="1253"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013262"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013290.1">
        <property type="protein sequence ID" value="ENSLOCP00000013262.1"/>
        <property type="gene ID" value="ENSLOCG00000010821.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163414"/>
    <dbReference type="InParanoid" id="W5MY03"/>
    <dbReference type="OMA" id="GHRNYMC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010821">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000026">
        <property type="entry name" value="BET1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="20">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="115" mass="12818" checksum="08DB38E259BF9C15" modified="2014-04-16" version="1">MRRAGLGDGAPTGNYVASGYSVHEEENENLSEGLRAKVSALKHLSIDIGTEVKYQNKMLSDMCTDSDFESTGGLLGATMGRLKLLSRGSQTRLFCYLLLFALFVFFVLYWVIRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYM8</accession>
    <name>W5MYM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Reticulophagy regulator family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031361">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013487"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013516.1">
        <property type="protein sequence ID" value="ENSLOCP00000013487.1"/>
        <property type="gene ID" value="ENSLOCG00000010995.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685264"/>
    <dbReference type="KEGG" id="loc:102685264"/>
    <dbReference type="CTD" id="79137"/>
    <dbReference type="eggNOG" id="ENOG502QPTN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164110"/>
    <dbReference type="HOGENOM" id="CLU_036265_2_1_1"/>
    <dbReference type="InParanoid" id="W5MYM8"/>
    <dbReference type="OMA" id="EKWKPRF"/>
    <dbReference type="OrthoDB" id="10029527at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010995">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052114">
        <property type="entry name" value="ER_autophagy_membrane_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057282">
        <property type="entry name" value="RETREG1-3-like_RHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952">
        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF4">
        <property type="entry name" value="RETICULOPHAGY REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="7">
        <location>
            <begin position="57"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="544" mass="59261" checksum="D4F46233847A6908" modified="2014-04-16" version="1">MASGEEVGAAVGRDPFMSSSTDALEALFPAGEAERAAGDGSPELRRLRERLQGLLAQYELAVLWVQRLLVWERPLHSIVVACTLNGVFWLLSSTTLRPLFLLSLSLLGLVLLERWKDKLPAITVRYPEASGSDTENIAVHPRLLSVPELSHHLAESYLTCSLYIQEMLQYKRLNHGKFCAMMCAGCSILAVVGHYIPGIMISYIILLSVLLWPLVVYHELIQKMYTGLEPILMKLDYSMKGGTQHRKHEKKKMKKEPEGSEEALAETESESEEELSCFSPTVDVKTAALALAITDSELSDEEASILESGGFSVSRATTPQLTDVSEDLDQQSMNSEPEETFSKDLPEFPSVDEYTSIEQGSPRGCVGQQLETALAAPPGPGGQSPAGFLIQSLASPLHFVNTHFNGHGRPPGTGEEAQGQGLSLEALSEEIVSTAISTVVQNTLSALLRSSEASEGPSIAEFLPPEPQTPLTETDLKEETESPAHEATAPGGLEEEDFELLDQRELDQMDAELGLSSEPREEEQELTVAQPTETPSSCPQTEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYV6</accession>
    <name>W5MYV6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Solute carrier family 24 member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYV6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013565"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013594.1">
        <property type="protein sequence ID" value="ENSLOCP00000013565.1"/>
        <property type="gene ID" value="ENSLOCG00000011046.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685443"/>
    <dbReference type="KEGG" id="loc:102685443"/>
    <dbReference type="eggNOG" id="KOG1307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234532"/>
    <dbReference type="HOGENOM" id="CLU_007948_5_1_1"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 4 other cell types or tissues"/>
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        <property type="entry name" value="Na_Ca_ex"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="135"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="175"/>
            <end position="199"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="205"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="239"/>
            <end position="258"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="471"/>
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        <location>
            <begin position="499"/>
            <end position="522"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="601"/>
            <end position="624"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="631"/>
            <end position="650"/>
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        <location>
            <begin position="140"/>
            <end position="281"/>
        </location>
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    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="17">
        <location>
            <begin position="500"/>
            <end position="648"/>
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            <begin position="307"/>
            <end position="334"/>
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    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="457"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="ARBA" id="ARBA00005364"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="661" mass="73395" checksum="EE702EB5D5D8DBCD" modified="2014-04-16" version="1">MGLNLPGSDVLKEFFTDMGPLDCKRHRHIRRKLRPVRILGLVLSLLAISGASFSFSALTWTTRHTTELFSTTDSQQSGARPHRTLLGTEDQQDQNVSSGLPVAMTQTVENNKTEPVHQGDYPEDLFSLEERKRGAVVLHMFGMLYMFIALAIVCDEFFVPSLTVITEKLAISDDVAGATFMAAGGSAPELFTSIIGVFISHSNVGIGTIVGSAVFNILFVIGMCALFSKEILNLTWWPLFRDVSFYIIDLIMLIVFFLDNYIHYWESMFLLAAYSTYVVFMKYNSNVEGVVKNIINRNKVMEVADVQAKPSTDPPGDENKLTLKPRLQRGGSSASLHNSLMRNSIFQLMIHTLDPLAEEFADSELGTYGKLKYYHTMTEEGKFREKASILHKIAKKKCQVEDGEKVNGVANRADKVDLPNSTNVEVEVTPPMQNGNVLHGIEAEGQGGDDGEDDEDQPLSLSWPETNRKRVTYLLILPIVFPLWLTLPDVRKLTSKRFFPITFFGSICWIAGFSYLMVWWAHQVGETIGITEEIMGLTILAAGTSIPDLITSVIVARKGLGDMAVSSSVGSNIFDITVGLPLPWLLYSFINNMAPVAVSSNGLFCAIVLLFLMLLFVIGSIAACKWKMSKLLGLIMFLLYFVFLVVSVMLEDKILVCPISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ88</accession>
    <name>W5MZ88_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase</fullName>
            <ecNumber evidence="19">2.7.1.60</ecNumber>
            <ecNumber evidence="20">3.2.1.183</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">UDP-GlcNAc-2-epimerase/ManAc kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>UDP-N-acetyl-alpha-D-glucosamine + H2O = aldehydo-N-acetyl-D-mannosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30683"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17122"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="3.2.1.183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:30684"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an N-acyl-D-mannosamine + ATP = an N-acyl-D-mannosamine 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23832"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16062"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57666"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.60"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23833"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amino-sugar metabolism; N-acetylneuraminate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Homotetramer. Homohexamer. The hexameric form exhibits both enzyme activities, whereas the dimeric form only catalyzes the phosphorylation of N-acyl-D-mannosamine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.60" evidence="19"/>
    <dbReference type="EC" id="3.2.1.183" evidence="20"/>
    <dbReference type="EMBL" id="AHAT01031602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ88"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013697"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013726.1">
        <property type="protein sequence ID" value="ENSLOCP00000013697.1"/>
        <property type="gene ID" value="ENSLOCG00000011146.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUGI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011146">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009384">
        <property type="term" value="F:N-acylmannosamine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24060">
        <property type="entry name" value="ASKHA_NBD_ROK_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03786">
        <property type="entry name" value="GTB_UDP-GlcNAc_2-Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000060">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000013">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000015">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020004">
        <property type="entry name" value="UDP-GlcNAc_Epase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03568">
        <property type="entry name" value="NeuC_NnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964:SF149">
        <property type="entry name" value="BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE_N-ACETYLMANNOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="ROK (REPRESSOR, ORF, KINASE) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="25">
        <location>
            <begin position="54"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="23">
        <location>
            <position position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="598"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00071183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR620004-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR620004-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="739" mass="81106" checksum="C5B8A6B9759486E2" modified="2014-04-16" version="1">DLYYVSIQRQKEQMEQKEKNSRKRKLRICVATCNRADYSKLAPIMCGIKADPDSFDLEVVVLGSHLIDDYGNTYRMIEQDDFDISSKLHTIVRGEDEAAMVESVGLALVKLPDVLHRLQPDVLIVHGDRFDALALATAAALMNIRILHVEGGEVSGTIDDSIRHAISKLAHYHACCTRSAEQHLIAMCEDHSRILLAGCPSYDKLLSARDRDDYMDIVQNWLGENVKEQNYIVALQHPVTTDIKNSIKLYELTLDALISFNKRTLILFPNIDAGSKEMVRVMRKKGIERHPNFRAVKHVPFEQFIQLVAHAGCMIGNSSCGVREAGAFGTPVINLGTRQTGRETGENVLHVRDADTQNKIYHALELQFGKRYPCSKIYGDGNAVTRILKFLKTIDLDEPLQKTFCFPPVKDCISQDIDHILETQSALSVDLGGTNLRVAIVSMKGSIVKKYTQANPKTYEARIELILKMCTDAVIDAMSINCRILGVGVSTGGRVNPQEGVVLHSTKLIQEWSAVDIRTPISDKLHLPVWVDNDGNCAALAEKKFGHGRGVENFVTIITGTGIGGGIIQHSELIHGSTFCAAELGHIMVSLDGPECMCGSRGCIEAYASGMALQREAKKLHDEDLLVVEGMSLKNEDPVSAIHLITAAKMGNSKAEAILKTAGTALGVGIANILHTVNPSLVILSGVLASHYENPVKQIISQRALNSVQGTKVMTSDLEEPALLGAASLVLDYATRRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N012</accession>
    <name>W5N012_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">T cell receptor gamma variable 10 (non-functional)</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N012"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013971"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014000.1">
        <property type="protein sequence ID" value="ENSLOCP00000013971.1"/>
        <property type="gene ID" value="ENSLOCG00000011369.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SQHK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153143"/>
    <dbReference type="HOGENOM" id="CLU_077975_7_0_1"/>
    <dbReference type="InParanoid" id="W5N012"/>
    <dbReference type="OMA" id="THILLHM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011369">
        <property type="expression patterns" value="Expressed in intestine and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001649">
        <property type="entry name" value="T cell receptor gamma, variable 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-1279">T cell receptor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866819" evidence="11">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="34"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="124" mass="14183" checksum="234985BD8E7C2182" modified="2014-04-16" version="1">MFLVSCISVSSLFLVTKAIITLEQVKISQTKNIGKTVYFSCKVTGTDFNKAYIHWYQKKAGKALKHLLYIRAEDGVLHYDDENDKEIFKASQNEDIAESDFIINSLKVDHSATYYCSCWDSHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0L4</accession>
    <name>W5N0L4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ku70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01031112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N0L4">
        <property type="interactions" value="1919"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014173"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014202.1">
        <property type="protein sequence ID" value="ENSLOCP00000014173.1"/>
        <property type="gene ID" value="ENSLOCG00000011533.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153239"/>
    <dbReference type="HOGENOM" id="CLU_014815_2_0_1"/>
    <dbReference type="InParanoid" id="W5N0L4"/>
    <dbReference type="OMA" id="SEQGRVC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011533">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="X-ray repair cross-complementing protein 6 isoform X1"/>
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        <property type="entry name" value="SPOC-like_C_dom_sf"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="17">
        <location>
            <begin position="295"/>
            <end position="418"/>
        </location>
    </feature>
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            <begin position="465"/>
            <end position="500"/>
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        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="420"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
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            <begin position="1"/>
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            <begin position="13"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
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    <feature type="active site" description="Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity" evidence="14">
        <location>
            <position position="30"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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        <source>
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        <source>
            <dbReference type="SMART" id="SM00559"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="502" mass="56373" checksum="88D847369D0FAEC1" modified="2014-04-16" version="1">MAEWDDYYRHEGEEEAEQEEGEESGAGDYKFSGRDSLVFLVDASKEMLVESEDDITPFEMTLQQCVRSVYTSKIISSDRDLVALVFYGTAKHKNSVSFKHVYVYHDLESPGARRVQELDRLQGERGRQLAKEELGCSPDFSLGEALWACSNLFSDVKLRLSHKRLMIFTNQDDPHGGDRTRDRLARTKAADLKETGEGGWGVCSVFTGVISVINKSSEQGRVCRVFTGVMSVINKSSEQGRVCTRIPLSLGEGVSLAVGVYVLARTATKPPAVRLYRDTNEPVRTKTRLYHAHTGSLLLPSDTKRSQTYRDRQIVLENEEVAELKKFEEPGLVLIGFKPLDRLKRHHHIRPALFIYPEEQQVTGSSKLFTALLKRCSERGVFAVCRYTPRRNTPPRXEPKLDVMDQRLGVLAQEFKELVYPPDYNPEGKGPAQKRKPEGGGGAEKKARVEVSEEGLRAHLEKGTLGKLTVPVLKDACQQLGVRTTGTKKQELIDALTKHYSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1T0</accession>
    <name>W5N1T0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inositol 1,4,5-trisphosphate receptor-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus outer membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ITPRIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1T0"/>
    <dbReference type="FunCoup" id="W5N1T0">
        <property type="interactions" value="1138"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014589"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014618.1">
        <property type="protein sequence ID" value="ENSLOCP00000014589.1"/>
        <property type="gene ID" value="ENSLOCG00000011865.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRDF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="HOGENOM" id="CLU_025485_2_0_1"/>
    <dbReference type="InParanoid" id="W5N1T0"/>
    <dbReference type="OMA" id="CHLHCLQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011865">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026250">
        <property type="entry name" value="ITPRIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF8">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02107">
        <property type="entry name" value="INOS145TPRIP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="15">
        <location>
            <begin position="395"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="541" mass="62211" checksum="2D40469C01029DD8" modified="2014-04-16" version="1">MQGTVVRVCVVVAAAILNHPLLFPRENTTIPERDDQVIARMREHEEKLLAEKARLEREASQVEQGVQPSPDVEESSSWDLWSALSMIVFLMIEIWRQDYHDTTAQDPTYEEEDSLPLGNVSLKIPLPDKAVLSCFYERCIRISANETWRVQDFVEGFADDLLEALRSVCNRDADMEVEDCIGVGSMYENWRVSKPLICDLIVPFAAPDPYCFTFQLWCNSQNDIPPDRQGYGRIKVVRANEDGPGCVCGKTDLGEDMLCLLHSKNERPKVSDAMDDLLCSKNTPYLAKDQVMKWFLISMTKAWNCISHKYDFELMFRNLDSPGALKVKFRSGKTIIFNITPVVQFEDTDAYFVSHFAFNNDNPSDIFWPLSFAVYEKNLMKVLVKRLPESSCHICCLQIISFLHKKQTSLTGESALTNYHLKTALLHLLLSKPSSDWRAEYLEKRLRDVLRFLEKCLQEKKLYHVLIGNKQVPKHINFPPVFQAAEPLNLFRPLVLQRDLYAKTVEHFQEMLKNAPVLIQEYTPLFPNGSHHIDFNHGAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2K0</accession>
    <name>W5N2K0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Golgi transport 1A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in fusion of ER-derived transport vesicles with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2K0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014859"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014888.1">
        <property type="protein sequence ID" value="ENSLOCP00000014859.1"/>
        <property type="gene ID" value="ENSLOCG00000012079.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014507"/>
    <dbReference type="HOGENOM" id="CLU_124519_2_0_1"/>
    <dbReference type="InParanoid" id="W5N2K0"/>
    <dbReference type="OMA" id="FFFQRPK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012079">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF245">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN GOT1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="133" mass="14709" checksum="80DDDD689BE8EC7A" modified="2014-04-16" version="1">MITVTDVQKLGVGLTAFGLFFLMFGVLLYFDSVLLAFGNILFLAGLAVIIGLRRTFQFFFQRHKLRGSAFFLGGVALVLLRWPVVGMVMETYGFVLLFRTFFPMAFGFLGSLVNIPFLGSLLNRLSGSSSSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2M2</accession>
    <name>W5N2M2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endoplasmic reticulum protein 27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01039185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2M2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014881"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014910.1">
        <property type="protein sequence ID" value="ENSLOCP00000014881.1"/>
        <property type="gene ID" value="ENSLOCG00000012093.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151058"/>
    <dbReference type="HOGENOM" id="CLU_088451_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2M2"/>
    <dbReference type="OMA" id="EESHGYK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012093">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF253">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23460" checksum="7F34DE7EC369A481" modified="2014-04-16" version="1">GFQEFLSVASRLQTLPFALCSEPEVWQLYNITGDTVSIFRQTDSHQENLQLQERMKIDSDGLTRFIRTNELYYLTEYNDMTAVGLFSSPVRAHLLLLADKRSAGFAQLRELLRALAPQYRGQLLFVLIDGAVSSNRRSLEYFGLKSHDLPAVGLYDTQTDRKWLMQAQEVTTEHVQEFCYSYLRGDLQEQNDPPTSAATHSKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N345</accession>
    <name>W5N345_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01016655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628529.2">
        <property type="nucleotide sequence ID" value="XM_006628466.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198039.1">
        <property type="nucleotide sequence ID" value="XM_015342553.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069046488.1">
        <property type="nucleotide sequence ID" value="XM_069190387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N345"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015054"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015083.1">
        <property type="protein sequence ID" value="ENSLOCP00000015054.1"/>
        <property type="gene ID" value="ENSLOCG00000012243.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689660"/>
    <dbReference type="KEGG" id="loc:102689660"/>
    <dbReference type="CTD" id="8444"/>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159878"/>
    <dbReference type="HOGENOM" id="CLU_000288_5_13_1"/>
    <dbReference type="InParanoid" id="W5N345"/>
    <dbReference type="OMA" id="HNGTSQT"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
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    <sequence length="581" mass="65061" checksum="1AD0289DEAC83C09" modified="2014-04-16" version="1">MMILSRKPDGPITAARHADGLYDSYMRTEEVGTEDADARPHSPSALPPLPKHTGGSKTTAKDHHVIGSRGGQMKVKQLYEDSYNRKPNSVAQHNGTHASSKPLPALSKERSVDSTSSAKSGDSMTRSSKPGPLSPEQALKQHRQQLTALEQQEIYNYPEIYFVGPNAKKRAAVAGGSNNGGFDDDQGGYIHVPHDHLAYRYEFLKVIGKGSFGQVAKVYDHKLHQHLALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVVHMLENFTFRNHICMTFELLSMNLYELIKRNKFQGFSLPLVRKFAHSILQCLEALHRNKIIHCDLKPENILLKQQGRSGIKVIDFGSSCFEHQRVYTYIQSRFYRAPEVILGSRYGMPIDMWSFGCILAELLTGYPLFPGEDEADQLACMMELLGMPPQKLLEQAKRAKNFINSKGHPRYCTVTTHSNGTVVLNGSRSRRGKMRGPPGSKEWVTALKGCDDPTFIDFLKKCLDWDPSSRMTPSQAMRHPWLYKRLPKPISGEKTLAPKRIAEPSTSFPGIISKLPPAIGVANSKLRANMMGDSGGSIPLRTVLPKLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N483</accession>
    <name>W5N483_LEPOC</name>
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        <recommendedName>
            <fullName evidence="9">Synaptopodin 2-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Actin-associated protein that may play a role in modulating actin-based shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015442"/>
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        <property type="gene ID" value="ENSLOCG00000012538.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183054"/>
    <dbReference type="HOGENOM" id="CLU_007120_1_0_1"/>
    <dbReference type="InParanoid" id="W5N483"/>
    <dbReference type="OMA" id="PGPYQGV"/>
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        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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    </dbReference>
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        <property type="term" value="C:Z disc"/>
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    </dbReference>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10820">
        <property type="entry name" value="PDZ_SYNPO2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Synaptopodin 2-like a"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOPODIN 2-LIKE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
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            <begin position="139"/>
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        </location>
    </feature>
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    </feature>
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            <begin position="583"/>
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    </feature>
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        <location>
            <begin position="721"/>
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    </feature>
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    </feature>
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        <location>
            <begin position="935"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="937"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="994" mass="106044" checksum="F732EE07341C2BC5" modified="2014-04-16" version="1">VMVVEEAVITLSGGAPWGFRLQGGADHRKPLLVAKVRKRSKACRAGLCEGDELLSINEKPCGGLSHAQAMNLIDSCPGVLHIRVKRAPTGFQSVVLVPRAPSPRIDKEYRAALRALSPPQPTGPEVTRARLMSPTLRGPEALPEECAHRDCLTSPPDSEAYYGETDSDADTAHEKQRRQKRRSPSSSPGKASGKASPAETSEAGNSAENSSASTDDQSPRDGQGAVEVDSGFQEPPNPLPPLVSPERARGVSLLLSGSRQLVPMVGPVDNPVDEELTVTYMDKAKQAKLHRSESVQEKQVKEARTKCRTIASLLTDAPNPHSKGVLMFKKRRQRAKKYTLISYGSVEEDQLRADMEGDEDEEDGVLPTSESEFDEDGFAAAADADTTWDSDYLDILEKKDPASQRSSSPGAPESPGLPGSAGRGAQLFEQQRKRAEEHTVTGSVPEVAQMPREAGTPNTQPSTPITPPSYSVMNGDISPKSRSAELMSPPHLAPMPLPDLPSSSVLNRTARPFTPGFVSSRAATAPVIFRPTSARKTPSQPPSSQPMASVPAPFSAPPASAPKLAISTASLYIPARSASAVAPLSVLSPPPPFSPPPAPAAPLPSSLQPPAAAPPVAGPIAPPAYSAPSSTEALVSREQRISVPAARTGILQEARRRSTKKPMFSRPEEKKGSSPNPELLSLVQNLDERPKGVTEAGFESGPEEDFLNLGAEACNFMQAQKHKVPPPVAPKPRIHVPEIPQLGGKGAELFARRQSRMDRFVVDTSPQQTSQPASPARPRLPSPTPSLPSHWKYSSNIRAPPPIGYNPLLSPSCPPGAQRSTKAAEAPKTGTWSGRKTSQKEGIKAIDFIRRQPYQLNPAMFSFGSSGSSVAQQSRPLMGSSLTQPRQIPVKAARVYEIKRFSTPTPMSVPTSLTPTVIAPRAATTLGEPLFRSNICSPPPTTTAPPPASAPAPAGTPVQEAKQYQTTPEVPKPRFQASKIGIQSNVWRPGALRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5B4</accession>
    <name>W5N5B4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger and BTB domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5B4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015823"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015853.1">
        <property type="protein sequence ID" value="ENSLOCP00000015823.1"/>
        <property type="gene ID" value="ENSLOCG00000012855.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160246"/>
    <dbReference type="HOGENOM" id="CLU_407055_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5B4"/>
    <dbReference type="OMA" id="CRIDNDT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012855">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18196">
        <property type="entry name" value="BTB_POZ_ZBTB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000061">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="188"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="371"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="66523" checksum="ED821E64CB9DF832" modified="2014-04-16" version="1">MDFPGHFDQIFQQLNYQRLHGQLCDCVIVVGSRHFKAHRSVLAACSTHFRALFTVAEGDSSMNMIQLDSEVVTAEAFAALVDMMYTSTLMLGESNVMDVLLAASHLHLNAVVKACKHYLTTRTLPMSPPGERPPPEQQQQPASSRLQRSFLLQQLGLSLVRKWCRVPVKRSVSSCVKLVWQETGAGISEAPYGGPQEDAQMPSQSDSGRAGSGPRPQPQAGQGAGDDFRDPQQELPGDGGKEKEAEEEEHMRVVVKSEPLSSPEPQDETSDVTSQAEGSDQVEPGADKIELSPESSDRSFSDPQSSTDRVGDMHLLEGNDNLDTKQAFSISSFLAKGYAGPGQGSASLTDDNLPNTTSAECRIEGEATYLLSPEPINGGSSSGGSLLHHHPASSSHHHHHHHLSGEVNPFGESDGHFLRPMQEGLGYRGGEQFALDFQRSSLGLHSFCRSGRGGGASANLGFPGYRRIAPKMSPAVGGSLRMEGTQLQDACGGPHLLLNGAAGGGSGYEGGPHGAAAGGAGAAAPPQLTRASADVLSKCKKALSEHNVLVVEGARKYACKICCKTFLTLTDCKKHIRVHTGEKPYACLKCGKRFSQSSHLYKHSKTTCLRWQNSNLPNTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5M2</accession>
    <name>W5N5M2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5M2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015931"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015961.1">
        <property type="protein sequence ID" value="ENSLOCP00000015931.1"/>
        <property type="gene ID" value="ENSLOCG00000012938.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502T804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2984231_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5M2"/>
    <dbReference type="OMA" id="VYIAIKV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012938">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001131">
        <property type="entry name" value="pro-neuregulin-3, membrane-bound isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040180">
        <property type="entry name" value="Neuregulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100">
        <property type="entry name" value="HEREGULIN-NEUREGULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100:SF18">
        <property type="entry name" value="PRO-NEUREGULIN-3, MEMBRANE-BOUND ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="58" mass="6524" checksum="3D032393F2BDDAEF" modified="2014-04-16" version="1">SVLVYIAIKVCLSSSAPTYPPEHSEHFKPCRDKDLAYCLNEGECFVIETLTGSHKHCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5X2</accession>
    <name>W5N5X2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Thyroid hormone receptor alpha a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028975">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5X2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016031"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016061.1">
        <property type="protein sequence ID" value="ENSLOCP00000016031.1"/>
        <property type="gene ID" value="ENSLOCG00000013012.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157917"/>
    <dbReference type="HOGENOM" id="CLU_007368_18_0_1"/>
    <dbReference type="InParanoid" id="W5N5X2"/>
    <dbReference type="OMA" id="IMCLRIA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013012">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002154">
        <property type="term" value="P:thyroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06961">
        <property type="entry name" value="NR_DBD_TR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06935">
        <property type="entry name" value="NR_LBD_TR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000006">
        <property type="entry name" value="Thyroid hormone receptor beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000011">
        <property type="entry name" value="Thyroid hormone receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF42">
        <property type="entry name" value="THYROID HORMONE RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="162"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="47119" checksum="D0040E181BFAA25E" modified="2014-04-16" version="1">MEHMPKEKDPNLSEGEEKRWLDGSKRKRKNSQCSVKSMSGYIPSYLEKDEPCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCISVGMAMDLVLDESKRVAKRRLIEENRERRKKEEMVRTLQNRPEPTSSEWELIRMVTEAHRHTNAQGSHWKQKRLPLQPEDIGQSPVAPTSDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGKSLAQFNLDDTEVALLQAVLLMSSDRSGLTCVDKIEKCQETYLLAFEHYINYRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5Y3</accession>
    <name>W5N5Y3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma</fullName>
            <ecNumber evidence="10">3.1.4.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the rod/cone cGMP-PDE gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.35" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01000404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635232.1">
        <property type="nucleotide sequence ID" value="XM_006635169.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211359.1">
        <property type="nucleotide sequence ID" value="XM_015355873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5Y3"/>
    <dbReference type="FunCoup" id="W5N5Y3">
        <property type="interactions" value="880"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016072.1">
        <property type="protein sequence ID" value="ENSLOCP00000016042.1"/>
        <property type="gene ID" value="ENSLOCG00000013026.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692424"/>
    <dbReference type="KEGG" id="loc:102692424"/>
    <dbReference type="CTD" id="554700"/>
    <dbReference type="eggNOG" id="ENOG502S20G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013260"/>
    <dbReference type="HOGENOM" id="CLU_170469_0_0_1"/>
    <dbReference type="InParanoid" id="W5N5Y3"/>
    <dbReference type="OMA" id="WAARMEN"/>
    <dbReference type="OrthoDB" id="8525078at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013026">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045745">
        <property type="term" value="P:positive regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1120.10:FF:000001">
        <property type="entry name" value="retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1120.10">
        <property type="entry name" value="Retinal cGMP phosphodiesterase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006952">
        <property type="entry name" value="PDE6_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037030">
        <property type="entry name" value="PDE6_gamma_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE GAMMA-SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122:SF4">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04868">
        <property type="entry name" value="PDE6_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000969">
        <property type="entry name" value="35-cGMP_Pdiase_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 10" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="87" mass="9582" checksum="20C808BFC4864571" modified="2014-04-16" version="1">MNLEPPKPEIKSATRVSGGPATPRKGPPKFKQRQTRQFKSKPPKKGIQGFGDDIPGMEGLGTDITVICPWEAFSHLELHELAQYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6F1</accession>
    <name>W5N6F1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interferon phi 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6F1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016210"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016240.1">
        <property type="protein sequence ID" value="ENSLOCP00000016210.1"/>
        <property type="gene ID" value="ENSLOCG00000013155.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107079272"/>
    <dbReference type="KEGG" id="loc:107079272"/>
    <dbReference type="CTD" id="360134"/>
    <dbReference type="eggNOG" id="ENOG502S7K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000050089"/>
    <dbReference type="HOGENOM" id="CLU_121486_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6F1"/>
    <dbReference type="OMA" id="YRFTGIC"/>
    <dbReference type="OrthoDB" id="8924072at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013155">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002312">
        <property type="term" value="P:B cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002323">
        <property type="term" value="P:natural killer cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002286">
        <property type="term" value="P:T cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060337">
        <property type="term" value="P:type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867104" evidence="8">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="183" mass="21443" checksum="E8ED5BA916C37684" modified="2014-04-16" version="1">METTSLWKCLFLLLCSQTCALGCRWIANGYNIVSRDALSLINKMGGEIVVDTGDVRFPQPLYNRARKMSMEHKIAFVNENFRHLLHLYAGNLDSVTWDRSLLNEFTEVLHRQAREMRSCAGTKQAPKKQHKKLQAYFKKLEKSILKKTKYSADAWEIVRHHVIRHLQELNRLANLMRKEMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>W5N783</accession>
    <name>W5N783_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Interacts (via Sushi domain 21) with ITGA9:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction. Interacts (via Sushi domain 21) with ITGA4:ITGB1; thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction. Interacts with ANGPT1 and ANGPT2. Interacts with PEAR1 (via extracellular domain). Interacts with HSPG2, TLN1, FN1, COPA, CCT2, IQGAP1, LAMC1 and NID1. Interacts (via C-terminus) with TIE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N783">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016492"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016522.1">
        <property type="protein sequence ID" value="ENSLOCP00000016492.1"/>
        <property type="gene ID" value="ENSLOCG00000013366.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685314"/>
    <dbReference type="KEGG" id="loc:102685314"/>
    <dbReference type="CTD" id="79987"/>
    <dbReference type="eggNOG" id="KOG1217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156061"/>
    <dbReference type="HOGENOM" id="CLU_000343_0_0_1"/>
    <dbReference type="InParanoid" id="W5N783"/>
    <dbReference type="OMA" id="KPLPLCE"/>
    <dbReference type="OrthoDB" id="6515930at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013366">
        <property type="expression patterns" value="Expressed in mesonephros and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003013">
        <property type="term" value="P:circulatory system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009913">
        <property type="term" value="P:epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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            <begin position="2172"/>
            <end position="2199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2349"/>
            <end position="2376"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2407"/>
            <end position="2434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2465"/>
            <end position="2492"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2523"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2676"/>
            <end position="2703"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2734"/>
            <end position="2761"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2792"/>
            <end position="2819"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2850"/>
            <end position="2877"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2908"/>
            <end position="2935"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2966"/>
            <end position="2993"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3025"/>
            <end position="3052"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3083"/>
            <end position="3110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3260"/>
            <end position="3287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3318"/>
            <end position="3345"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3376"/>
            <end position="3403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="3434"/>
            <end position="3461"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="3554"/>
            <end position="3564"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="3572"/>
            <end position="3581"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="3586"/>
            <end position="3596"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="3604"/>
            <end position="3613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3624" mass="394668" checksum="FEED91D398ED13DA" modified="2014-04-16" version="1">MWRLLVFYFYLHAAGSLPVLPQFSLYKHPHLNLSHVLPLAEPRQQQESSESKVERLGQYFKRNVRTLREKSLSLDLVFLVDESSSVGNSNFLSELRFVKKLLSDFPVAPSATRVAIVTFSSKSNVVTRVDYISSPQPHQHKCALFNQEIPAIRYRGGGTYTKGAFQQAAQILRHSRQNATKVIFLITDGYSNGGDPRPTAASLRDVGVEIFTFGIWQGNIRELNDMASHPKEEHCYLVHNFAEFEALARRALHEDLPSGRYTQEDAARCSALCEARRDCCDVMASCKCGTHTGQYDCVCERGYYGKGLQHECTACPPGTYKPEGTPGGVSTCLPCPDKHHTSQPGSTSVKDCVCKDGHRPVGKSCEVVRCPEIHPPENGFFIKNVCNSHFNSACGIRCKPGFDLVGSSIRLCQPSGKWSGTDPSCKVRACPRLSKLKHGHIKCSAPDASYRTTCDVRCEEGYRLEGSARLTCQASGRWTSPVPQCVEIHCPPLQKPKNVLLLPPTCGDKEMKMGTTCQLSCHHGYSLSGETEVQCLTSGKWSTDVHRAICTDIEPPQINCPKDIVAETMEHHSSANISWIVPNARDNSGEEVSIQVTPVFIPPLLFRIGEVRITYTAEDGSHNKASCSFNVKVIDTEPPVIDRCRSPPPFHTSETEMTAVWDVPQFSDNSGAAVMISSTHSPGDLFPIGETVVQYTATDPSGNNRTCNIHIIIRGSFCEQPFHPVNGEFTCTEDEHGVNCTLYCKEGYSFTHEPIHSYYCANDGMWEPPYSAEFSDCSLNRIANHGYKPFEMLFKASRCDDINLLKAFSGEFYTTLQGEVPSLCSADNVVCKLETIPQGQCLEYNYDYENGFAIGPGGWGNGWGSQGGLDYAYTDSGFHSDQFASKQQGGASPKMVPFRRKRHQHQIPTRDQKIQIIFNITASVPLPSERNDTAEAENQKRLLQTLEKMTNRLKRTLSKQPLFTFRVASEVIVADTKSLESQKASLFCRPGSVLKGRMCVHCPVGTYFSLEYNACESCWIGSYQDEEGQVECKSCPAGFSTVYMHSKSAAECKAQCKPGSFSANGLETCESCPLGQFQPAFGSKSCEVCPKDTSTVNRGAVDVTECGVPCPAGQFSRTGLMSCYRCPRDYYQPEAGRSYCLSCPFYGTTTISGATAISDCSSFGSSFLPKEESVVVSVSPDSIRNSYQASSQVFHECFLNPCQNRGTCEEVGAGYICLCPLGFTGSKCESDIDECDSAPCQNGGICKDGMGEFFCHCQPGYVGMLCEAEVNECSSSPCVNDGVCVDEINEYSCNCAEGFTGVHCELEINECLSNPCENGATCEDLVGLFSCSCLPGFSGDRCEMNINECASTPCLNGGTCSDDINSFRCQCRAGYTGKMCEKDVNECDPNPCLNGATCADGLGSFTCKCLPGFNGTRCETEMSSRFNLDFEVSGIHGYVMMDNIMPSLTEITCAFWMKSSDTINYGTPISYAVEEGNDNTFLFTDYNGWVLYVNEKERITDCPSVNDGRWHHIGVSWRSADGDWKVYIDGNFSDGGKGLSTGTIIPGGGVLVLGQEQDERGEGFNPVESFVGSLSQLNIWDYVLTPQQIKELASSCPEELRRGNLLAWSDFLTGIVGRVKTIHNSVFCADCPPLEHSVPHLRVSSPSITAGSQVELSCDPGYVLVGERDQQCLNRGEWSHSPPQCERVSCGPPPPLEHGSHTGQGFQAGDSVTYKCNIGFYLLGDAKVLCANSGSWSGHPPACLDVDECALGSDCDKHSVCHNTDGSYTCTCIPPYSGDGRNCTEPVQCKDPGTPQFGHRDGNVYMVGGEVVFSCEEGYELIGSARILCLESGTWDLPAPYCKAVSCGRPSVPENAIMEGINFTYGSKVTFSCKKGFVLADEAEIYCQANMSWSKTPPLCQAITCGEPPHLENGNFTLNGSMYLSTVSYRCTEGYRLQGSAVLTCEMAGNWTGPAPVCEMVFCGEPPPVKDATIIGSNYTLGSKIYYTCKEGYTLIGLESKECLPTSEWSQSSPQCVPRSCGNPPPIDNAFPESGHQLYGDTAIYFCADGYSLADSSEVVCNAQGRWTPPDNKELPRCIADFCQRPPDVPHAILDSVNKPKYASDSVVSYKCEEGFMLNTTATLKCVRGGQWTPPPFDIQCIPVRCSRPSSVERGYVSGTNYSFGAVVAYSCDKGFYIRGEKKRTCEANGDWGGTLPTCQPVSCGEPPQLKNGFIKSKSSIVFESQVTYGCDPGYKLVGSPVRVCQANRQWFSESAPSCVLLTCDPPPTVAHGHYVGTNFEVGSKVEYICDEGYELSEDAIWTCLKYGKWSKTEAPVCTAVQCPEPPLEENHLVLKNIDAESGTIELSCDDGYVLQGSHILRCMHSKEWNSTFPLCKRVFCGTPPEVLFGHPSSAPFYFGSSVTYACMEGFTLRKESSVTCQADGTWSKPSPECIPVECPHPREIPNGIVDVQGLTYLSTAFYNCKPGYHLVGNSTVLCGESGLWIGAIPTCKPIECPKPKEIASGKVSYSKLQYNHEVAYTCNRGFRLEGPGILTCLETGQWDAETPVCREIHCVTPQPIENGFVEGIDHRFGSTIIYSCLPGFQLAGHALQTCEESGWSSSTPVCLPTDCGLPPHIDFGEYVKVLDPNRELEKEDMDDPGLTMSKPQNEPSSSLDLTDHMDIGFLHGSTVIYTCNAGYELIGNAMLVCQEDGLWNGTAPVCLLAECEPPLEPEHGFISLSDTALGSLVQYSCDEGYELEGQTVRQCVSGRQWSDTAPTCKAVSCGEPEGIPNGSVKGDTFTFTSIIYYECDTGFQLEGNNTSTCQANKQWYGKTPRCLPVSCGSPLLPDNGTVVGNEYTFGKQVIYSCNAGFVLEGATHSSCLANGSWSHATPVCRQAICIRPPDIANGEVLGSQSGYKSEVRYRCREGYSLRGKSLLICQGNGVWDSEPPHCVPITCDPPEDISHGYLNGSSFNFGEFVEYVCFPGYEVLGSSIMQCSANGSWTGTVPSCQPCLCQPPTIENGAALGNGHASVCGEEVQFQCEEGFKMLGPSKATCESGGVWSPGIPYCGESKCSSPPSVPNAIINGSSSIYPNAITYRCKPGFEMTGQSHLICKDSGVWSEPYPACEPVSCGPPPFVSNADVIGKEFTFGNTIHYRCLEGFELETKANSLSCLQDGTWSTHVIRCKHKTCPLPTNLTNIIITGTDFTTNSSIGLSCVEGFQLLRGSYSVCQPNGSWTPDVSSDSCIPVACKRPNPPMHGIVMGTRYNYRDSVVYRCHPGYELQGNPESICQANKLWSGTEPLCVKVSCGQPAAVENGLVQLKHFSGGDVAVHSCQPGFQLQGPRERICGASKQWSPSAPVCVRVSCGTPPTVENAVAMATGETYQSNVSFVCNSGFHLVGVQNITCLANGTWSNPTPQCEGIICESPRELLNGKVQFRNLTSGIAVQFQCDEGFRLEGEPLTYCLGNGTWSSPFPTCTRISCGLPLHVPNALVRGAHFQYGDMVMYSCFGGYIMKGSSRSACLENGTWTPPPACEAVCWSPCENGGICQRPNMCLCPEGWMGRLCEDPICILPCLNGGRCIAPYRCDCPDGWTGTRCQNAVCQNPCLNGGKCIRPNRCHCPPAWTGYDCSRKRKAGYYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7C0</accession>
    <name>W5N7C0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Atos homolog protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription regulator that syncronizes transcriptional and translational programs to promote macrophage invasion of tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATOS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015198519.1">
        <property type="nucleotide sequence ID" value="XM_015343033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7C0"/>
    <dbReference type="FunCoup" id="W5N7C0">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016529"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016559.1">
        <property type="protein sequence ID" value="ENSLOCP00000016529.1"/>
        <property type="gene ID" value="ENSLOCG00000013407.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686812"/>
    <dbReference type="CTD" id="56204"/>
    <dbReference type="eggNOG" id="KOG2306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157573"/>
    <dbReference type="InParanoid" id="W5N7C0"/>
    <dbReference type="OMA" id="QTHPRSQ"/>
    <dbReference type="OrthoDB" id="8625101at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013407">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033473">
        <property type="entry name" value="Atos-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025261">
        <property type="entry name" value="Atos-like_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051506">
        <property type="entry name" value="ATOS_Transcription_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199:SF13">
        <property type="entry name" value="ATOS HOMOLOG PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199">
        <property type="entry name" value="GH03947P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13889">
        <property type="entry name" value="Chromosome_seg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13915">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01177">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atos-like conserved" evidence="7">
        <location>
            <begin position="803"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="605"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="644"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1001" mass="111851" checksum="104DE2D8619E9A47" modified="2014-04-16" version="1">MKSDREAAEEYFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSGQPVNTKHECSENLPQCRQARRTRSEVMLLWRNNIPIMIEVMLLPDCCYSDEGPTTDGNDINDPAVKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGVVPRNILYRISAADEELVWNFSQPPTEHVFPVPNVSHSVALKVSVQSLPRQPNYPVLKCSIHTSLTFYGKKLQGQEERPITSVNRCNLQGSSLIYNKPAWSPSAETIVNPRKCPLPEVTSPSKAVRWFYAKLNNSSDIGSLLPKKQGYGTINGGEISKLPAFEPPVRSFKSLTLVDPLGPPSQGSRPPSGETNPLIGSLLQERQEVIARIAQHLNYCDPTAPHIPGSLFSNHEPHGSKAFWSLHEEDGFSKKSKEPGFLCHASQDVPSPENSYEGKSRSSPFDTPLNLCSKAKIDGGLRPSPKPVACRRLLLPESAENSRISEAVQDISRLIQDTFQEAQNKCNGLHNCTPKTCKEGTLKAPSTLERVCSSPDSQFKGQVPCHDTSQAFIKEISTDLQICTDYKSSRTKITDNNKHDCSIRLQNEACKKLEYEKNQSELGTSDSLHNAKKDPRNSFTEGHMERNEDIQGGNRFKVTDTSLRTDVANTKSPDQVDKSKPVQDENTDPTDSSTFAGLTQRVSHEDQTVKAERAPADVVRVKAQSPLKPFNIWKKQNRHSIDGTATNAFHPCTGLPLLSSPVPQRRTQTGCFDLDTSLIRCKHLPWSTNKRVCLKTIDRDDQGHVFSTSAPPTTSLSLLGNFEESVLNYRLEPLGTVEGFTAEVGASGMFCPSHMTLPVEVSFYSVSDDNAPSPYMGVITLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVIFDLRKMPANHQTFLRQRTFSIPVKREMNRHVTKKLPLLTEERTLRYLIHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSYTEAPSNPPFSPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7K1</accession>
    <name>W5N7K1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Zinc finger homeobox 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01033558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5N7K1">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016610"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016640.1">
        <property type="protein sequence ID" value="ENSLOCP00000016610.1"/>
        <property type="gene ID" value="ENSLOCG00000013472.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697503"/>
    <dbReference type="CTD" id="79776"/>
    <dbReference type="eggNOG" id="KOG1146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159542"/>
    <dbReference type="InParanoid" id="W5N7K1"/>
    <dbReference type="OMA" id="DESKTGM"/>
    <dbReference type="OrthoDB" id="6417226at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013472">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000082">
        <property type="entry name" value="Putative zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000317">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000768">
        <property type="entry name" value="zinc finger homeobox protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000058">
        <property type="entry name" value="zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000064">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000096">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051968">
        <property type="entry name" value="ZnFinger_Homeobox_TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891:SF2">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24056">
        <property type="entry name" value="zf-C2H2_ZFHX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1365"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1510"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1562"/>
            <end position="1591"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1893"/>
            <end position="1921"/>
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    </feature>
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        <location>
            <begin position="2061"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="2158"/>
            <end position="2218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="2247"/>
            <end position="2276"/>
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    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="2526"/>
            <end position="2586"/>
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    </feature>
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        <location>
            <begin position="2598"/>
            <end position="2626"/>
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    <feature type="domain" description="Homeobox" evidence="16">
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            <begin position="2850"/>
            <end position="2910"/>
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    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
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            <begin position="2063"/>
            <end position="2122"/>
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    <feature type="DNA-binding region" description="Homeobox" evidence="13">
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            <begin position="2160"/>
            <end position="2219"/>
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    <feature type="DNA-binding region" description="Homeobox" evidence="13">
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            <end position="2587"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="3280"/>
            <end position="3310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="3417"/>
            <end position="3432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="3461"/>
            <end position="3484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3538" mass="389291" checksum="69C125A53906AA5F" modified="2014-04-16" version="1">METCDSPSISRQENGQSISKLCETTRLDNEVPEKVAGMEPDRENSPADDNLRTDESRREIASGFASENVGVTQVASAKEIPCNECATSFPSLQKYMEHHCPNARLPLLKDENESEISDLEDSDVENLAGEIVYQPDGSAFIIEDSKESGQSAQPGAKSLFSPAMLANSQTASGDKTEQSAAAPMSFYPQVINTFHIASSLGKPFTADQAFPNTSAFAGVGPVLHSFRVYDLRHKSDKDYLTIDGAAKNSCVSKDVPNNVDLSKFDGCISDGKRKPILMCFLCKLSFGYIRSFVTHAVHDHRMTLNEEEQKLLGNKYVSAIIQGIGKDKEPLISFLEPKKSNSVLPHFSTANLIGPDPSFRSLWSAFHVENGDSLQAGFAFLKGSASTAVSADQSLRNIQMPKAEMNHGGLSVSAMKTPITSVSLSHSSPGSGGRDPDSNRDRQKEVSTLHSNGEFPIKVEPAEPVDGDEDEDAYSNELDDDGIGELADSTSSKDFPLLNQSISPLSSSVLKFTEKGTSSSSVTVSDDVEKTKQTTAHRDSSNDANNYSISGKDFADFGVGRDGAVASHLNDSTQGDAGSPVPPHQHIAAPGTPGAGEGSPGSGIECPKCDTVLGSSRSLGGHMTMMHSRNSCKTLKCPKCNWHYKYQQTLDAHMKEKHPESGGSCAYCRTGQPHPRLARGESYTCGYKPFRCEVCNYSTTTKGNLSIHMQSDKHLNNVQNLQNGGAEPVFNHSVPVSTASLTACGTPSPSKPKQKPTWRCEVCDYETNVARNLRIHMTSEKHMHNMMLLQQNMKQIQHNLHLGLAPAEAELYQYYLAQNIGLTGMKLENPADPQMTLHPFQLDPSTAAALAPGLVNNELPPEIRLASGQLMGDDLSVLSAGELSPYISDPLLKLFQCAVCNKFTSDSLEALSVHVSTDRSLPEEEWRAVIGDIYQCKLCNYNTQLKANFQLHCKTDKHMQKYQLVAHIKEGGKANEWRLKCIAIGNPVHLKCNACDYYTNSVDKLRLHATNQRHEAALKLYKHLQKQEGASGSESCCYCCGLCDYSSRAKLNLVQHVRSAKHQQSEALRKLQLHQQGLAAEEDSLSDVFFVKDGPPSEPEEPGEEPEGAFKLGGVAAAEERDTAERDGKEGRKTEKESRVNALGKDAPGASALGKRALQENEHDSGPKRPRSAEESKTSNDQVYQCPYCNYSNKDTNRIQMHVMSQHSMQPVICCPLCQDVLSNKIHLQLHLTHLHSVSPDCVEKLLSTVSGPDVMMPSNMLLPTNSQDKLQCQVDVASAATQEAPGKPSGDSAGEEKGPAAQDKGEQELQPDRLRPPEEASEAADWKKSGGSREARTPDPLQEHLSELQKRQPLSVSDRHVYKYRCSHCSLAFKTMQKLQIHSQYHAIRAATMCSLCQRSFRSFLALKKHLENGHPELSEAEVHQLCGSLSLNGDFSPESEARALEEVQALEQELDKDDDLDQEGKASPAGSDSSSLPDEMGSEPKRTLPFRKGPNFTMEKFLDPSRPYKCTVCKESFTQKNILLVHYNSVSHLHKLKKVLQEASSPVPQETNNSIDNKPYKCNICNVAYSQSSTLEIHMRSVLHQTKARTAKLETSSSSASGNTANSPVSVSQGNVDSASLPASANKENHIDAKEISKKQAPDPISVQPTHQPSQSPAQLQMQLQHELQQQAAFFQPQFLNPAFLPHFPMTPEALLQFQQPQFIFPFYVPGAEFNLSPELALHSAFGMPGMTGSLLEDLKQQMQQQHQAGQQQLQLLQQQAQQQQSSQSQMQQQKPQQQATKSAKVEQISTAIADSQMSKEAEELFEKQESKAKQEYLSDIESMKENKEAKKLKPQEPLIPPPRIVSGARGNAAKALLENFGFELVIQYNENRQKSQKKSRTVEDEIMDKLECGMCGKLFSNILILKSHQEHVHGQFFPYGQLEKFAQQYREAYDKLYPISPSSPETPPPPPPPPPPPPPPPQASGAGKVQAPTPTPLQAPPPPAPPPPPPPPPVAPQVQLPVSLDLPLFPPLMMQSVQHPALPPQLALQLPSMDTLSADLTQLCQQQLGLDPNFLRHSQFKRPRTRITDDQLKILRTYFDINNSPNEEQIQEMAEKSGLPQKVIKHWFRNTLFKERQRSKDSPYNFSIPPITTLEDIRLESQFSALEYYKTDPSHNKRSSRTRFTDYQLRVLQDFFDTNAYPKDDEIEQLSTVLNLPTRVIVVWFQNARQKARKSYENQSDAVKDSEKKELTNERYIRTSNMQYQCKKCSVVFPRIFDLITHQKKQCYKDEDDDGQDDSQAEDFMDNSDQMLPKHVSGLFDTTKPSLGTATSSGSSTPLMPSPKPDMGKTSPKPDFPTDKPKQAEAFPLQVSKAVPLSEPQPTQPSTPQPQQQKSQPQIVRPHSQPQTAVPSSPLSIAITSLTNSLPPQMLQYQCDQCKIAFPTVELWQEHQHMHFLAAQNQFLHSQFLERPVDMPYMIFDPNNPLMTGQLLSGALSQIPTQNSSALSTPTGASSSSLKRKLEEKEESSSNEKDGGNSGEDQHRDKRLRTTITPEQLEILYEKYLLDSNPTRKMLDHIAREVGLKKRVVQVWFQNTRARERKGQFRAVGPAQSHKKCPFCRALFKAKSALESHVRSRHWHEAKQAGYSLPPSPMMGVEDGGESPSKYSCFDYPPMLINKVEMTEYESPAAASTPSKQLEMPIKNFLGPSSLKAENSDDMEGLNMNSADVSYDQNKTDFDETSSINTAISDATTGDEGNNEVDSITGSACETKPALSPNDSKTHCGAEAKSNPTSNSDSYEERFPFALVSPSLSFSGKDSEQHYNMRDDEQDENADQSENSSLADPSSPSPFGSSNPFRSAKCSGERPGHKRFRTQMSNLQLKVLKACFSDYRTPTMQECEMLGNEIGLPKRVVQVWFQNARAKEKKFKINIGKPFMISQGGPDGPKSECSLCGVKYTARLSIRDHIFSKQHITKVEETVGSQIDRERDYLAPTTVRQLMAQQELDRLKKATDVLGLTTQQQNVVDSNALHGLSLPTAYPGISGLPPVLLPGVNGPSSLPVFPPNTPALASPGAGMLGFPTPATPSPAMSLSSAPAKPSLQTPAPPPPPPPSASQQTEQQSKDAEAEKKPDKAKKAKDREAENPKPETPAVKKREKSSPALSGMGKAGSEATMDPAQVQAVQNAIAGDPSSFLGGHFLPYFIPGFASCFSPQLPGGVQGGYFPPLCGMENLFPYSPAMPQAIAGLSSTALLQQYQQYQQSLQDSLQKQQQQQQKQLEQPQQKPVATKSSPAPASALKPTKEASETKEEKGSSTESTKEEPPTDTKSTDCPDAFIVPSIKHEFICRKCQMMFADEDSAVRHQKSFCYFGQPFSDPQETVLRNAVSKYNCLACDVAISGNEALGQHLQSSLHKEKTIKQAMRNAKEHARLLPHSVCSPNPNTTSTSQSAASSDNTYPHLSRLSMKSWPNILFQASARKAASSPSSPSPSLSLPSTVTSTSCSTSGVQTSLPTESCSDESDSELSQKLDDLDNSLEVKAKPTSGLDGNFNSIRMDMFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N805</accession>
    <name>W5N805_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 51</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N805"/>
    <dbReference type="FunCoup" id="W5N805">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016764"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016794.1">
        <property type="protein sequence ID" value="ENSLOCP00000016764.1"/>
        <property type="gene ID" value="ENSLOCG00000013595.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686947"/>
    <dbReference type="KEGG" id="loc:102686947"/>
    <dbReference type="CTD" id="79714"/>
    <dbReference type="eggNOG" id="ENOG502QWCS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164287"/>
    <dbReference type="HOGENOM" id="CLU_060968_0_0_1"/>
    <dbReference type="InParanoid" id="W5N805"/>
    <dbReference type="OMA" id="STATTWW"/>
    <dbReference type="OrthoDB" id="6243211at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013595">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037660">
        <property type="entry name" value="CCDC51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28624">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28624:SF1">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="403" mass="45606" checksum="83342D34F914BC41" modified="2014-04-16" version="1">MRYRVDLLPVRYGTRCYLAQVSGNLRPDVLPFRFYCAQNPKEAGGKLPLQSGLQKFANISRQWGQSSVKGASAAINNWWEKYEEFVGLNEVRDAQAKVTEAEQAFMVARGIVREAHESLEALQVRLKEVRDRLDRVSREEVHYLELATLEHKLLQEERRLRTAYRNAEEAEREKFALFSAGVRESHEKERARAERTKNWSVIGSVLGALIGVLGSTYINRVRLQELKSLLLEAQKGPVSLQEAIKVQASIHESQQEELGGLINSLRGTLQGPKPADKAGTAAIKILPSASDSDLKEQLLFSKKIHGLIEGIQPQLRKLEHEMGKVASELQTVKVVIRSRPTENQLLKSQDVQPYDTEGIMLELTQSEKRLEAQINRNTMYNTVLTYTAFALTLPVLYLIFKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8F4</accession>
    <name>W5N8F4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Krueppel-like factor 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8F4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016913"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016943.1">
        <property type="protein sequence ID" value="ENSLOCP00000016913.1"/>
        <property type="gene ID" value="ENSLOCG00000013716.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687553"/>
    <dbReference type="KEGG" id="loc:102687553"/>
    <dbReference type="CTD" id="28999"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156977"/>
    <dbReference type="HOGENOM" id="CLU_035818_0_0_1"/>
    <dbReference type="InParanoid" id="W5N8F4"/>
    <dbReference type="OMA" id="VQIQPIR"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013716">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21580">
        <property type="entry name" value="KLF15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000368">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF44">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="401" mass="44148" checksum="8F214D52DC802206" modified="2014-04-16" version="1">MVDHLLPTEETFPHTGSSVGYLADMVTDTSNYQLLPSPFSEDELSDSSSFRSCSSPDSQVLSSSYGSNSSVESQDSFVDYLLAQIDLGTTPGFSWDRKDSRSIVKEETFEFPVFSTDLKDPGTFQPTLEEIEEFLEENMEVVLKQEPEEQGKGSRGGSHVISAAQHKDQTGSSVTLTDTKSAQSRESVKKEEVASSPVSADGGVPVILQIQPIQIKQEQSPAVAAQHQPQSDIKIAQLLVNIQGQTFALVPQVVQSASFSTSSKFVRIAPVPIAAKPVGVGDGSQSTILMGQKFQKNPAADLIKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPFACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRTVRTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9A0</accession>
    <name>W5N9A0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9A0"/>
    <dbReference type="FunCoup" id="W5N9A0">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017209"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017240.1">
        <property type="protein sequence ID" value="ENSLOCP00000017209.1"/>
        <property type="gene ID" value="ENSLOCG00000013957.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155076"/>
    <dbReference type="InParanoid" id="W5N9A0"/>
    <dbReference type="OMA" id="YYFRPMS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013957">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF177">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="358" mass="39294" checksum="2B48C9571B3EC352" modified="2014-04-16" version="1">SVSLQTLHVLVNRKLFLFQNNKMMNYNNNILTAPHPSSVPCTGPSPPISTPSGPLLDRKAVGTPSSSVGVYQRRHSVTLPNSKFNQNQFINNSLKVEPSSTGGSSNKENRFRDRSYSETGDRLLPKSAGGNGSGQVNSSRYKTELCRPFEENGVCKYGDKCQFAHGIHELRSLSRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRGPPPLSASNKLDRPRLQHSFSFAGFPSNNGLQDSPTSVTPPPIFHADDLQDWPSNPFTYSSQELASLFGPSLGSQADPSAPAPPSPTTFFFRAMSESPQLFESPPSPQDSLSDQEGYQSSSGSLSGSESPVLDTTRRLPIFSRLSISDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA08</accession>
    <name>W5NA08_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc finger FYVE domain-containing protein 26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Phosphatidylinositol 3-phosphate-binding protein required for the abscission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abscission. May also be required for efficient homologous recombination DNA double-strand break repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with AP5Z1, AP5B1, AP5S1 and SPG11. Interacts with TTC19 and KIF13A.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022326">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022327">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017467"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017498.1">
        <property type="protein sequence ID" value="ENSLOCP00000017467.1"/>
        <property type="gene ID" value="ENSLOCG00000014174.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00920000149143"/>
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        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014174">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0048599">
        <property type="term" value="P:oocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd15724">
        <property type="entry name" value="FYVE_ZFY26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000295">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="TPR_ZFYVE26"/>
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        <property type="entry name" value="ZFYVE26"/>
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    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
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    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591:SF1">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01363">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF25569">
        <property type="entry name" value="TPR_ZFYVE26"/>
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    <dbReference type="SMART" id="SM00064">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="10">
        <location>
            <begin position="1815"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="607"/>
            <end position="628"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <end position="1290"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1755"/>
            <end position="1809"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00014373"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00025962"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017467.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2545" mass="285181" checksum="5913381820A08E21" modified="2014-04-16" version="1">MSAGHPIGREGETSQEKLFEFFRACLLQGEWELAQACVPQLKEWQGGPQVEELLQAITACPYQLRWETVGSPHRLAWLWLLVLEKWSQERIPVIARSELEFLLLLEELEDTVPKSVLKELYKAFLHSHSSGLPREKKKEMSPQNLSQDVLSCLWNLLVNKPRLAQALAACLLADGTDCAMMEYSACLQKMFTDFLLQAVGSLRSPGTGDGTAQQRHTAEQIYSVLSVLPYEPERQSREQQQLCEALWEASWGQDSSLLEQRVLSCLLRPRCHGLVVLYSSVATVRRKGKLLQDLHSAQDSKELYEAEKGLLGLFSHCDRTSAWQTIYFDCHSSGKHFLEQVLLTALSLIKREDFSSLDQLLKQEFQPLSRLLLLLGWTHCQSVESAKTLLCILHENKNLYCDSMLKEFGDGLSVQLGILEWCSENSNIPGKETLQLLHSLDCHSALFILHSLTSLPALQEGQVLDLLQGLAEPHSSGQSDTLSCASVVARRNVVLFQGFCAMKYAIYALCANAHRYLCCQVCTDWCSEVQSEESWPCQDHISAQGKPLLFQDYVSKCQSCLDAVPDIFRLELLENIYSLLFLCDDNVGMEKIEKLCDVHKGNLGEKKIQPTSLGRNEETASDSAQQSKVQTQGQNFRYLNLKQFNTGANGFLANEVTMEIFLEMLKENLKGINSNILLEDNKMLARETEFAECLNCSITMETFDSRFKRLSQHVSEAQWRFQIVTSNKTHVNCLMFGVTVVTYTLYAKSLAVSVRHGSMKRRVAGRHSAVQAECICSHNSEKVIRVRDLFGSAVDTASQREPEETALDTHQSLLVPMMLSPPDSLLVSCILRSNFAEGHQVAQMFGLESSVCYGELLFMERYQQVLDELAQVEQKIESQTAEGSGRRVSSGRSTLQAIGSAAAAGMVFYSISDVADKLLSSPGKQLPTLQESYWLNNMVLEAKSPLRGPLDELSSPGMAAFDLACTQCQLWKTSKQLLEIADRRLHSSLESRGLKADHTVTHPEGLRGFPAVLQQIGKILNHTPTAKGQAKLVPEGSEEKTWSPFSCSAVEVLLSCYPCLTEEGITAQLLTTQRFEQVVHTLAGAADVTGEMRGTGLLGALVEQASLRLAELEAHPVRSQMKQLLRSVDQHFQVQESAAHSVDYIRTFFDYVNTLAGVLVHSQSSESAELACEVKLGNPLLVLQQTPSQHLTHLLFERQVPPDRLSSLLYHEELNISVQQVIVQRCCESLPLWDTRRQRQDPALLDGITALLQQYAQPHVSHLGTTLPLLPKPSEESEEPSSPPLSPGGNSTQLFLTPSALSFLKSCSSLLAVVACLSTSKGTRVAKSGLSSWREFRGKREAPLDMEQISKECEQLLREFPVLQGFLHVICEPVVGNQLEGSGLVAALCGKSSTSLAFSGLHCGSTRNVATDAFQQALSSKDFGRALCVLELYGQEHKGVGPLKDIVLSCAAVEAEPDGIKHVFRVKHPDLRARLTLECLKKWPLEASLEVLLYCLSATETAPELKGKLELKKQELQVYQKILSLSDTLPWTEWQDLQRDSTRIPEVVMFVVLRANEFELCEKWVELHPISPELLLRLQTDHLLHLLDRGLTEEAFEELLESLSSPALRLQVSERALDQRPGLAVCHFLADYLTSQFQSSMTLARQCEIQAMRIGSKLLLLLPEVARQDYFHLLCSPLLMLEQLLMNMKVDWAALAVRTLQQLLVGQEAGFTGEDIDSLLSNYAHKALEFPFSLRERTRSDSVISLQDLLNQNPGQDSFSSAPAGESVSPPISGGTLLQKPSTPRDRERMTHRLKPPTEFTPPGKPPDKRNWVPDHSQCVCMACRKEQFTMFNRRHHCRRCGRLVCNSCSSRKMRVEGCRDEAVRVCDQCYNFFYQPAEEESEPEEVSGSPCPGDVGFASILQLPEMPLRQHKLGLNEAENEIERSEFYYEQAPSASLCVAILNLHSNHIACGHQLIDQCRELSQGLNNPEVDARLLIDIMKQLLFSAKVMFVKVGQSQDLALCDSYISKVDVLKILVTANYKYIPSLDDILQPAAVTRLRNQLLEAEYYQLAVEVSTKSALDPSGVWHAWGMATLKAGNLTVAREKFSRCLKAPLDLNQLTQGSRLLQDIVQHLESNVKTPHSLPDNDILASLRELEEALTETAGTDKPDGRIQQSSSYQECLYYLHAYGTNLAITSFYMRHDCMREALLHLLNKESPEEVFLEGIFITCYERGKLHLLENLLESLDSSLESWSRYLIAACQHLQKRSFFNILYELQQFMKDHVRAAMTCIRFFTHKAESYVDLGEKQKWLIKAKDHLKTYLQEHQGRSSARRKSPSTFRKKMSSTDMSRHINTIELQIEVTKFLQRCESSGTSRITSLPPATLFGNSLMKEDVACKVMLGGKNIEEGFGVAFRVIQDFQLEAGTVYTKAAKRLVKQRQYKEIRQLLKCVSESGAATKNDCDDIILSCVVAADKGPANAKELESLILEMKNTDSKIKAYLLCSKLRPAYLLAVKLELPQATQLVQEVLQAAESDRDTMMQTICQQWLSEHQGKAAVRESRAGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA94</accession>
    <name>W5NA94_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inactive tyrosine-protein kinase PEAK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudopodium-enriched atypical kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Sugen kinase 269</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tyrosine-protein kinase SgK269</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NA94">
        <property type="interactions" value="619"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017553"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017585.1">
        <property type="protein sequence ID" value="ENSLOCP00000017553.1"/>
        <property type="gene ID" value="ENSLOCG00000014257.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVUZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157591"/>
    <dbReference type="HOGENOM" id="CLU_002882_0_0_1"/>
    <dbReference type="InParanoid" id="W5NA94"/>
    <dbReference type="OMA" id="MGWNRNR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014257">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043535">
        <property type="term" value="P:regulation of blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000145">
        <property type="term" value="P:regulation of cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000437">
        <property type="entry name" value="Pseudopodium enriched atypical kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051511">
        <property type="entry name" value="MitoQC_Scaffold_Kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972:SF5">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE PEAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1303"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="31"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="126"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="216"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="357"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="500"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="724"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1011"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1188"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1396"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="512"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="580"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="871"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="921"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="934"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1081"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1221"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1409"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1432"/>
            <end position="1448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1759" mass="191003" checksum="8E517524573A3FFD" modified="2014-04-16" version="1">MSACNTFTEHVWKPGECKNCFKPKSLHRLTEGVLKKGAQEQQQNQSTSGGKPNANLSNNYQRGSAPVRSGHFRPPVAKKPTIAVKPTMMLSCSATIDSEGNHGQADSGELGKTSAFAVWNRNGMNRKRPGGVNNNEGEESGEEIEGYGLRSSRAPSGNNNNGLTEVLKEIAGLGSGPGSSSGSSSKDLFLGRISSCYKRSLERGLPASSCLAMGSGTGASQKHVSLSGSTEVISSEGGRFCYPDFSSDGEEDEEESDEEEEGEHESWDESDEELLAMEIRMRGQPRFANFRASTLSPVPFAVGKKWNTVPLRNRSLQRICAVDYDDSYDEILNGYPSEDSNGGHILGPYAQSDLQGSCFFSNSETTTSPESSSSLPEDSRTTSSSGSSAPCHLPNGLPSSAASREPDFESVSEKEQVPRAAKSPKASETHKAILAIRLEEQDAALKSPSLPQALPGQPVTISFSPGEEQAKPYRVVSLEKPAMCKPYTVVDVSASMASTESQNQDAVSKMKGSSVSSTPPSTPSSPLGQPLSPKSKSSAIRYQEVWTSSTSPRQKIPKVELVGGCAAGPAVPYRHCSHKSAPTSPTTGLSSSSRTVPVKSPNLSEIKFNSYNNAGMPPFPIIIRDEPTYARSSKNAVKVPIVINPSAYDNLAVYKSFLGLSGEQSQSKPGLESRVTSHTYEEIGSSENIPSSPTETPLNGKVVKEKAVGEESTTNAIASITAVTTQGGSQAKTSITVATPGHNWKVSGETIPRKDGNSEPQMSSGPGTAHREKASTVLSQIVASIQPPQSPPESPTVQNKTCSAEELYSIPPDATKEAPSRPKSLFSPAEGCLPKPRKDTPTRQLPKSQNSTAVPSASPKSENAPFPPPRSTSSPYHASNLLQRHFGNWSKPTNNSSSRPNEAESAQAGEGRRTAAESAKPKRWISFKSFFRRRKDEDEQQREKPEREKEKGKLVGLDGTVIHMLPPPPVQRQHWFAEAKTDDPNQKPTIIFTYKPEMSPATEGEPVQICEEENKDNKIFKSLESQVPGEKNESGVPAAASLPAKSEFPQRDEQVSAATPASVSEGTGSLEEREEECSDSPASSTCSATYSNLGQSRANMIPLKQPRNPKTSNDTLASADLDGSDTGSKATPPPLPKKTITRANTEPSALGKDAATTLKPKAEAKPGGTNLSVANPLYDLDSTWETASQSSSLSSEARLLDQESGDSLERPAVGKGRPANSLSCLTSSSATSAGREKRGYRSAESLAGRGRTASRGGVPKPQRQVLYKGMENWEEVVGRIRGLHTDTLRKLASKCEDRFMAGQKDQLRFGTDSWSDFRLTTGKPCCEAGDAVYYTASYAKDPLVNYAIKICRSKVKETQQQFFHSLAVRQSLAVHFNIQQDCGHFLADVPVRLLPWEDPNSPEEEDEEKVGKQSEKTRKKTEKPAEPKMPSHSAQTPGHPSDINTSGKLRSRVVVITREVPYQTVADFVREGVTRHERNPDLYERQVCLLLLQLCSGLEHMKPYHVTHCDLRLENLLLVQCQPGNPWNLDVMEPNNNNNTGGGMSTSAANSACPARLIISNFSQAKQKSQLVDPDVLKDQSRLAPEIITATQYKKCDEFQTGILIYEMLHQPNPFEETPELKEKEYTRADLPPLPPRSLYSQGLQQLASLLLNANPSERIQISDARACLQCLLWGPREDLFQTLNASPGPGHRETTLQNWLDLKRTLMMIKFAERSLDAGCGVSLEDWLCCQYLAFSTTDSLSRVVQILQQNQKQQQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB13</accession>
    <name>W5NB13_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mastermind like domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB13"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017822"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017854.1">
        <property type="protein sequence ID" value="ENSLOCP00000017822.1"/>
        <property type="gene ID" value="ENSLOCG00000014480.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV1F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111366"/>
    <dbReference type="HOGENOM" id="CLU_298909_0_0_1"/>
    <dbReference type="InParanoid" id="W5NB13"/>
    <dbReference type="OMA" id="FNNAAWV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014480">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026131">
        <property type="entry name" value="MAMLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15275">
        <property type="entry name" value="CG1 PROTEIN/F18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15275:SF0">
        <property type="entry name" value="MASTERMIND-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="854"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="936" mass="98641" checksum="F326F1777D8A6336" modified="2025-10-08" version="2">MQLQGSVKRKLADDSSPTVNGLSDGMIPGNSKRPCLEDVTLSMGPGSGSYSSGTGLGGHSGLLDGNQLNGNSISSHFSVPPSADIKPSPSAAGHGGMLPPFDPNGNNRAPSVEQELQDILDELTKNPDPSLHELDIEKILGNKSDDQVNVSFMHPDNNCTPKRSPHRTSHLESHLTSKDFSPGFSQAPVGSPQVGPPPSGPPYSLSHTAKAVPSPLSSSSQNQSQSQARSPMLSAALSSRPGTNWHEVSRAQQLQQMASNKHISTPPQQPSAQTSNWPAMPSSGPSPPYREKLSSSSPHQQPFSPANSMQSPQSAIISSMAPAPSTGPSPPYRPEKLASPALAQPPFSPQNSLLPSMAPTGGTIQSPQANLLPSMPSSGTRPSPPYRPDKLSSPAVQQPSFSPQNGSGATMSSQLFKAITSSQSTSLNMLMQQQQQQMPQGLLTKGAMGQDPFSFNNTKPLSHFDPDPPPTQKMASIPSGPGQPSLSHYMSQSSSSGIPQAPSASHPQLLQQQRMQRSLEPGGLVPHSRTEQSPAMAPRLQDPSSMVRASPRPGQGNGYSLLFKTQFMRKQAQSLDVKKRKSQRLCILHTPANLVSFSPSGAGVNFPSRQIQKLSGVGRSLPGDCGYSMPNPPASSSMLSQNPMLANRMGMPAGPLSQPSRAIAAFPGNPGAKQGMYPPPSSDFNMPLRPSQSMLGMGMHSQTPARTAVAASSFGASTLAQPHLRHALGQQGGSLPRVMVNPQMWPAQQGVPRMSSDAQMDHSLQPFPGSPMFPQQSMRPGMPAPYPHHPGAPPNQIAPNLPVRQMQKPSPAQPGPALSSLAAHSMRTRGPLSAMAGMKPVPPGMAGLNQVHPAQGLAPPSYPTSSKHPGGYSTGNPGAKLPQYDFTQHQNNGTMPGPGGSGANISEVDFIDTLVGSNEDWLNNLTMIDEYLEQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBV3</accession>
    <name>W5NBV3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NALCN channel auxiliary factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632889.1">
        <property type="nucleotide sequence ID" value="XM_006632826.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBV3"/>
    <dbReference type="FunCoup" id="W5NBV3">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018112"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018144.1">
        <property type="protein sequence ID" value="ENSLOCP00000018112.1"/>
        <property type="gene ID" value="ENSLOCG00000014712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699087"/>
    <dbReference type="KEGG" id="loc:102699087"/>
    <dbReference type="CTD" id="27112"/>
    <dbReference type="eggNOG" id="ENOG502QQKW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161362"/>
    <dbReference type="HOGENOM" id="CLU_058453_0_0_1"/>
    <dbReference type="InParanoid" id="W5NBV3"/>
    <dbReference type="OMA" id="PSPHCTD"/>
    <dbReference type="OrthoDB" id="10047996at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014712">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF8">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="400" mass="44857" checksum="F2148564C39F81D8" modified="2014-04-16" version="1">MIRGAWMYRREDDAVLKICCAPKQNDKPCADSERAQKWRMSLASLLFFTVLLSDHLWLCAGVKLRCKDRGFRKAWSKATDGDHLVLNEGKCDIFLSNSTKSAAPSPHCTDAQNHQDLESACTKLRLQKPGFGSSFTSPSSLSSRLLLAYFRNFSLSFCDSYTISDLLLGMANPDSLNCSLQNLIWDLGRGGGDDWDVCSNCIQAYMRLDQHAQEKYEEFDFLFLKYLSEEYSVRSCIGDCKSVYKAWLCSEYFNATHSQCHHRIPCKQYCLEVQTRCPFVLPDNDDLVYGGLSSFICTGLLENHLTNAEPECCDVRWSSCDPSVDGVCNASTKFMDSTTSHHRSSLPVSAASRLCNSRLKLCVLVLILLHTVVTFSTIQNNGGLSLEALTAMEDSSTREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC16</accession>
    <name>W5NC16_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC16"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018175"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018207.1">
        <property type="protein sequence ID" value="ENSLOCP00000018175.1"/>
        <property type="gene ID" value="ENSLOCG00000014762.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159563"/>
    <dbReference type="HOGENOM" id="CLU_2222325_0_0_1"/>
    <dbReference type="InParanoid" id="W5NC16"/>
    <dbReference type="OMA" id="ICDCSHE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014762">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF21">
        <property type="entry name" value="HISTONE DEACETYLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="106" mass="11044" checksum="F53F6742E562CDA4" modified="2014-04-16" version="1">MKDADYIAAFQQLLLPVAYEFQPQLVLVAAGFDAVIGDPKGGMAASPQCFSVLTHLLQGLAQGRVLLALEGGYNLQSTAEGACACLRSLLGDPCPRLAPPGAPCDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND04</accession>
    <name>W5ND04_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-responsive element-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629138.1">
        <property type="nucleotide sequence ID" value="XM_006629075.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND04"/>
    <dbReference type="FunCoup" id="W5ND04">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018513"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018545.1">
        <property type="protein sequence ID" value="ENSLOCP00000018513.1"/>
        <property type="gene ID" value="ENSLOCG00000015029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689982"/>
    <dbReference type="KEGG" id="loc:102689982"/>
    <dbReference type="CTD" id="3658"/>
    <dbReference type="eggNOG" id="KOG0452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157796"/>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_1"/>
    <dbReference type="InParanoid" id="W5ND04"/>
    <dbReference type="OMA" id="VYEPMFD"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015029">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030350">
        <property type="term" value="F:iron-responsive element binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000011">
        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000012">
        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000005">
        <property type="entry name" value="Iron-responsive element-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="78"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="217"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="775"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="146"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="974" mass="106249" checksum="087862BD5CA96F2E" modified="2014-04-16" version="1">MALGAVPEDHPFKDLIDTLESDGNENRFFFNPQKLNDPRYDLLPLSIRVILEAAIRKCDGFYVKKEDVFNILDWCQQQSSAEVPFSPARILLQDFTGVPAMVDLAAMRDAVKEQGGDPSQVNPLCPTDLIVDHSIQIDFSKCAIQNAPNPGGGEGPGAPARSSTAKPQPRRSQCGSQKSCKGSCKDGQGAGTSGRPTAVQIENTPILCPFHLQPVSEPEVALKNQEMELSRNKERLQFIKWCSEAFKNVNVVPPEMGAVHQVNLEYLSHVVQDDGRFLYPDSVVGTDSHTTMINGLGILGWGVGGIESEAVMLGQPVSLALPQVVGCKLVGSINTLATSIDIVLGITKHLRQAGIAGKFVEFFGPGVSQLSAGDRTTIANMCPEYNATVSFFPVDHITLKHLKQTKFDKEKLELMEGYLKAVKLFRSYEDPSEDPHYSEVIEINLSSIIPHVSGPKRSQDRVAVTSMKQDFLNCLNEKVGFKGFHVPSEKQNTVVSFQHEGAEYELAHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEAGLVVKPYIRTSLAPGNGMVTHYLSASGVLPYLSQLGFEVIGYGCATCVGNTAPLPNAVIEAIKEGDLVACGVLSGNRNFEGRLCECVRANYLASPPLVVAYAIAGTVGIDFEMEPLGKNSDGREIYLRDVWPTREELQQTEEQSVIPSMFRELKERMEKGNKLWNGLEAQESVTFSWDPKSTYIRCPSFFQKLTKEPAAPQSIENAHALLFLGDMVTTDHISPAGSIARTSAAAKYLMSKRLTPREFNSYGARRGNDAVMTRGTFASIKLQNRFIGKTGPKTLHIPSEQILDVFDAAEKYQREGIPVIILAGKKYGSGSSRDWAAKGPYLLGVKAVIAESFEQMHKNHLVGIGIAPLQFLPGESADVLGLSGKEKFSVSIPEDLSPRQELTVKASTGKTFKVIALFESEMDIVFYKHGGILKYVARKMLQHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND76</accession>
    <name>W5ND76_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pleckstrin homology domain containing A2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01006189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND76"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018585"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018617.1">
        <property type="protein sequence ID" value="ENSLOCP00000018585.1"/>
        <property type="gene ID" value="ENSLOCG00000015100.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV0M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158064"/>
    <dbReference type="HOGENOM" id="CLU_055135_1_0_1"/>
    <dbReference type="InParanoid" id="W5ND76"/>
    <dbReference type="OMA" id="CEPSIMD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015100">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13271">
        <property type="entry name" value="PH2_TAPP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000049">
        <property type="entry name" value="pleckstrin homology domain-containing family A member 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000042">
        <property type="entry name" value="pleckstrin homology domain-containing family A member 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF5">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="189"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="426" mass="48040" checksum="116AF1ED9BF409AF" modified="2014-04-16" version="1">MPYVDRQKRVCGFLDIEENENSNKFQRRYFILDTQVNSLLWYMDNPQNLPCGTEPVGNLQLQYISKVSEATVKQKPKAEFCFVINALSRRYFLQANDPDDLRNWVEALNKATKITVPKPGSIPQTAEITRVATDLQPKKQQAYKTEIIGGVVVHTPIIQNEGEEMDLGEGLPHGSTRRPAPSSAGVRPGVVKSGYCVKQGNVRKSWKRRFFTLDNSSVSYFKCETDKDPLRSILLKDIQKVHECLVKSGELLMRDNLFEIITSSRVFYIQTYTPEDMHGWIKAISGAIQALRGPVKVLVDYIVNPHNLYDSGFTRSSSLYRYQSGGARGWQGREDKKAPLVKSSSVAPSWQPWTPVPSAVSKASPEMTSVEEVTGQFSSLPTVHKGESEDQDSLGKRRRHRSQPQPPSEKIFPFNVDDDGIRTTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDA7</accession>
    <name>W5NDA7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Malonyl-CoA-acyl carrier protein transacylase, mitochondrial</fullName>
            <ecNumber evidence="3">2.3.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Mitochondrial malonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">[Acyl-carrier-protein] malonyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type II malonyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01025607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDA7"/>
    <dbReference type="FunCoup" id="W5NDA7">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018616"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018648.1">
        <property type="protein sequence ID" value="ENSLOCP00000018616.1"/>
        <property type="gene ID" value="ENSLOCG00000015128.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102687285"/>
    <dbReference type="eggNOG" id="KOG2926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013715"/>
    <dbReference type="HOGENOM" id="CLU_030558_2_3_1"/>
    <dbReference type="InParanoid" id="W5NDA7"/>
    <dbReference type="OMA" id="PLCEIAN"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015128">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.250:FF:000005">
        <property type="entry name" value="Malonyl-CoA-acyl carrier protein transacylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052760">
        <property type="entry name" value="Mitochondrial_malonyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170">
        <property type="entry name" value="MALONYL-COA ACP TRANSACYLASE, ACP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170:SF2">
        <property type="entry name" value="MALONYL-COA:ACP TRANSACYLASE (MAT) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="16">
        <location>
            <begin position="3"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="26531" checksum="175137AA8EB3A848" modified="2014-04-16" version="1">MLVAIERCVAATGFSLGEFAALVFAGALDFAEALYAVKVRAEAMQEASDRIPSGMMAVLGKAQAKYGYACLMAREHCESLGIESPVCSISSYLFPDGRVIAGHLQALEFLQKNSRKFSFLRTKLLPVSGAFHTALMEPAVEPLREVLKKVEIRKPEISVYSNVDAKKYMHGQHIRRLLLKQLVSPVKWEQTVHAIYERRQGAEFPETYEVGPGRQLGSALQKCNLKAWQGYRHIDVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDZ8</accession>
    <name>W5NDZ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytotoxic granule associated RNA binding protein TIA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">RNA-binding protein TIA-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">T-cell-restricted intracellular antigen-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homooligomer; homooligomerization is induced by Zn(2+). Interacts with FASTK; the interactions leads to its phosphorylation. Interacts (via RRM1 and the C-terminal glutamine-rich (Q) sequence) with SNRPC/U1-C (via N-terminus); thereby facilitating spliceosomal U1 snRNP recruitment to 5' splice sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01006102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193092.1">
        <property type="nucleotide sequence ID" value="XM_015337606.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDZ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018857"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018889.1">
        <property type="protein sequence ID" value="ENSLOCP00000018857.1"/>
        <property type="gene ID" value="ENSLOCG00000015324.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698667"/>
    <dbReference type="KEGG" id="loc:102698667"/>
    <dbReference type="CTD" id="7072"/>
    <dbReference type="eggNOG" id="KOG0148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154962"/>
    <dbReference type="HOGENOM" id="CLU_025000_2_0_1"/>
    <dbReference type="InParanoid" id="W5NDZ8"/>
    <dbReference type="OMA" id="RAIVQMN"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015324">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097165">
        <property type="term" value="C:nuclear stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12615">
        <property type="entry name" value="RRM1_TIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12618">
        <property type="entry name" value="RRM2_TIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000951">
        <property type="entry name" value="nucleolysin TIA-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000038">
        <property type="entry name" value="Nucleolysin tiar isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000045">
        <property type="entry name" value="Nucleolysin tiar isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034827">
        <property type="entry name" value="TIA-1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034830">
        <property type="entry name" value="TIA1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="8"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="96"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="204"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="41996" checksum="7E978DB3519F22E4" modified="2014-04-16" version="1">MMDEEQPKTLYVGNLSRDVTEALILQLFSQIGPCKSCKMIVDTAGNDPYCFVEFYEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYETNAKHLSFDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFIRFNSHEGAAHAIVSVNGTSIEGHVVKCYWGKETPDMMNPMQQVQVPQQNKVGFAQPYGQWGQWYGNAQQIGQYVPNGWQVPTYGVYGQAWNQQGFNQTQASAAWTGANVRVEPTQGLNGCVIANQAGFGTAGYQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEG5</accession>
    <name>W5NEG5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pyroglutamylated RFamide peptide receptor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEG5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019056.1">
        <property type="protein sequence ID" value="ENSLOCP00000019024.1"/>
        <property type="gene ID" value="ENSLOCG00000015458.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286926"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_0_1"/>
    <dbReference type="InParanoid" id="W5NEG5"/>
    <dbReference type="OMA" id="WSKPAWE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015458">
        <property type="expression patterns" value="Expressed in zone of skin and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001556">
        <property type="entry name" value="Bombsn_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF164">
        <property type="entry name" value="PYROGLUTAMYLATED RFAMIDE PEPTIDE RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00358">
        <property type="entry name" value="BOMBESINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="62"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="342" mass="39117" checksum="910846294D27DACA" modified="2014-04-16" version="1">FYMMNFTPQMLTFWLEASNVSRHQFIQEFGLSPLVYVPRLPSALQPIFSILYVLIFILALAGNTAVLFLLCRRKALQAPSTFFVCSLALSDLLISIFCVPATLLQHFFTNWLAGEFLCKLVAFLQVTAISASIFTMICIAAERFQGILYPLQLQNGYFPSHAIKMLAGVWLAALVIACPMWYAHKVEVKYDFLFDVHYTCCQEIWPHTWQRQAYTTFLLLLVFLVPMVTMSVLYGKIMRELWGKHRVHDVMFQTLPGSEINKITRKKRRAVKMMATVVLLFAVCWAPFHLVSVLYDYGKLSLDPDQEFLVFTTVQILGFGNSICNPVVYAVLNKNFKKGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NH73</accession>
    <name>W5NH73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Armadillo repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">In association with mitochondrial contact site and cristae organizing system (MICOS) complex components and mitochondrial outer membrane sorting assembly machinery (SAM) complex components may regulate mitochondrial dynamics playing a role in determining mitochondrial length, distribution and motility.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with mitochondrial contact site and cristae organizing system (MICOS) complex components IMMT/MIC60 and MICOS10/MIC10. Interacts with mitochondrial outer membrane sorting assembly machinery (SAM) complex components SAMM50 and MTX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NH73"/>
    <dbReference type="FunCoup" id="W5NH73">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019982"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020015.1">
        <property type="protein sequence ID" value="ENSLOCP00000019982.1"/>
        <property type="gene ID" value="ENSLOCG00000016210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694723"/>
    <dbReference type="KEGG" id="loc:102694723"/>
    <dbReference type="CTD" id="321240"/>
    <dbReference type="eggNOG" id="ENOG502QU5Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164948"/>
    <dbReference type="HOGENOM" id="CLU_077781_0_0_1"/>
    <dbReference type="InParanoid" id="W5NH73"/>
    <dbReference type="OMA" id="LFLDHQN"/>
    <dbReference type="OrthoDB" id="17335at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016210">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016617">
        <property type="entry name" value="ARMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46840">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46840:SF3">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013899">
        <property type="entry name" value="UCP013899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR013899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="271" mass="30107" checksum="0FC6979F0FF59DCE" modified="2014-04-16" version="1">MMDALAVVKQLRDLASEPQNREAIVRDQGCLPGLVLFLDHQDAQVLFSTLQTLRYLAECPSNVDTMKNELGMIVSLRNLMEREGPNNDIPFLAKEVYEVLTSPINTEPRTPDGPKRKAKPQFFINSSNKKAKSVTLHIQGLDSMDHRGLCEEALLRVKGVISFTFQMALKRCTVRIRSDLPTESLASAIAATKVLTAQQIVKNETGEEVLIPLRATNATVQENSSLPDYLPEDESPEKEADKAISRTGAKEDSNGSWLNAAASFLTKTFYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHW8</accession>
    <name>W5NHW8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POC1 centriolar protein homolog B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat POC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633617.2">
        <property type="nucleotide sequence ID" value="XM_006633554.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHW8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020227"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020261.1">
        <property type="protein sequence ID" value="ENSLOCP00000020227.1"/>
        <property type="gene ID" value="ENSLOCG00000016379.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687766"/>
    <dbReference type="KEGG" id="loc:102687766"/>
    <dbReference type="CTD" id="282809"/>
    <dbReference type="eggNOG" id="ENOG502QSVJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160413"/>
    <dbReference type="HOGENOM" id="CLU_000288_57_17_1"/>
    <dbReference type="InParanoid" id="W5NHW8"/>
    <dbReference type="OMA" id="QCRAYRF"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016379">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000235">
        <property type="entry name" value="POC1 centriolar protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF1">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="225"/>
            <end position="266"/>
        </location>
    </feature>
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        <location>
            <begin position="267"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="429"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037984"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="488" mass="53702" checksum="5BF1D06C6E06B272" modified="2014-04-16" version="1">MASVLEDPTLERHFKGHKDAVTCADFSPTNKQLATGSADTFLMIWNLKPKARAFRFFGHKDVVSSVQFSPSGSLVASGSRDRTVRLWTPSIKGESTVFTAHTATVRSVSFSQDGQKLVTASDDKSLKVWSVHRQRFLFSLNQHTNWVRCARFSPDGRLIVSCGDDRSVRLWDTTSRQCINTFTDYGGSAATFVDFNSSGTCIASSGADNTLKIWDIRTNKLLQHYQIHSAGINCFSFHPSGNYLITASSDSTVKIVDLLEGRLIYTLHGHQGPILAVAFSRGGDYFASGGTDSQVLVWKTNFDAFNYKDVLKHNRSRVNPDPPPHLTNIYPRSPHSHTVSPSSIQVNPSVADTQTVDPHVVEIGQSLYPKMGGSSHENGVPASRGWPGGRSFQPPHRSSGGQEEEGETCAVTFPLEEHTGFPSNFASTLEHIVEQLDVLTQTVAVLEKRLTLTEDKLKECLENQAQIVLQVREAEEREAESGSCNSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI73</accession>
    <name>W5NI73_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033542">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NI73"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020366.1">
        <property type="protein sequence ID" value="ENSLOCP00000020332.1"/>
        <property type="gene ID" value="ENSLOCG00000016460.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYH5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000186894"/>
    <dbReference type="HOGENOM" id="CLU_031817_0_1_1"/>
    <dbReference type="InParanoid" id="W5NI73"/>
    <dbReference type="OMA" id="NERCAAM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016460">
        <property type="expression patterns" value="Expressed in ovary"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052560">
        <property type="entry name" value="RdDP_mobile_element"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688:SF1">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="55"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="411" mass="46386" checksum="9794A3B589580715" modified="2014-04-16" version="1">DSNIKIYGYHLIGAIHHAYHGVATLVKLDRNAKHVAQSPDEDTTQWIAIEIEGFTIVNIYKPPSTQFSNLPSYPHPVIYAGDFNCQHTNWGYQKNTPEGKALADWASIQGLTLLFDNKQANTFFSARWQSGTNPDLVFFSPTNNHMPSPQRSVLSIFPNSQHRPVVTFHPALVTPTPSRPSARQTSSFPLKLPDPNSTDINDSYSRFCNMIISTSNNHIPRGFRKTYIPGWDEECTNLASELESATTTERAQSIGSTLIEYLDNIRKQEWNNMMVSEIDMKHSSIRAWQTINRLTGQSHKPQIQTTSKPKTSQSGKPTHTDKQFARKVKQEIHDSWRASSADDNSHSGFTYPELITAISTLKAGKAPGPDHLHSEQLIHLFVPALEWLLAFFNNCLLKYIIFPSLRFQANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ14</accession>
    <name>W5NJ14_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Potassium voltage-gated channel subfamily KQT member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">KQT-like 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Voltage-gated potassium channel subunit Kv7.1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025760">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025763">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025767">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ14"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020623"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020658.1">
        <property type="protein sequence ID" value="ENSLOCP00000020623.1"/>
        <property type="gene ID" value="ENSLOCG00000016693.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102687840"/>
    <dbReference type="eggNOG" id="KOG1419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166088"/>
    <dbReference type="HOGENOM" id="CLU_011722_8_3_1"/>
    <dbReference type="InParanoid" id="W5NJ14"/>
    <dbReference type="OMA" id="SICAATC"/>
    <dbReference type="OrthoDB" id="8879391at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016693">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086089">
        <property type="term" value="F:voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902282">
        <property type="term" value="F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098915">
        <property type="term" value="P:membrane repolarization during ventricular cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097623">
        <property type="term" value="P:potassium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000113">
        <property type="entry name" value="Potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
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        <property type="entry name" value="Ion_trans"/>
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        <property type="entry name" value="KCNQ_channel"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="27">
        <location>
            <begin position="131"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="28">
        <location>
            <begin position="557"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004240"/>
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            <dbReference type="ARBA" id="ARBA00004285"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009499"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019342"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="704" mass="78970" checksum="4FFF0E6DEFFE6AAD" modified="2014-04-16" version="1">MGDGEGFPMYDVGSNKAGSTRAQLSAQVTGPNGHSRNAQSPETSREPADMRYSPVSLEQDPAVNNGSGIPSSPQTDTKDPAFQIDSQRSSASELRRNYSPKHFHHPYLTKTSMQGDVYNFLERPSGLKCFLYHFTVFLMVLVCLIFSVLSTIEQYAEFASGTLFWMEIVLVVFFGSEYVVRLWSAGCRSKYVGIKGRLRFARKPISIIDLIVVIASVIVLSVGSNGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVYLAEKDAVDEEGKTGFASYADALWWGVVTVTTIGYGDKVPQTWIGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASLIQTLWRCYAAEKPGGCPATWKIYVMPCEYLPTITPEPSSPNLRKLSKRYKRKLKTSRDNGSTSLMNPGENALSIPQITYDHIEEKDGKREIAFYLDEPAGQQLSWSSLSLYSASCPSSPVKKQLEGPPIDISRTNSFVDEMDFVGEDIPLPVVTDASHLLTSPFMAKIQDSLSTQQLNSTRLTEAHRAAVKVIRRMQYFVARRKFQQARKPYDVRDVIEQYSQGHLNMMVRIKELQRRLDHSLGKPGMFLSEKGLDKGYYTVGARLIRLEDKVTQMDQKVEDILKILMEQFKPQPELLQKPSTPVSSRLSVMKRMGCTFDDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ23</accession>
    <name>W5NJ23_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01025757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ23"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020632"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020668.1">
        <property type="protein sequence ID" value="ENSLOCP00000020632.1"/>
        <property type="gene ID" value="ENSLOCG00000016697.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160161"/>
    <dbReference type="HOGENOM" id="CLU_013477_4_1_1"/>
    <dbReference type="InParanoid" id="W5NJ23"/>
    <dbReference type="OMA" id="FANAHAF"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000192">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF50">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="19">
        <location>
            <begin position="397"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="526" mass="60353" checksum="6989F5B1EEB3453A" modified="2014-04-16" version="1">PRDTKVETNQEKRVFKKLFPKSNLFEKWGDHLSEVEQKKAEWLYKRYGYNVYLSDKMPLDRKLPETRDPSCLGKKYPRDLPTLSVVLIYLDEALSIIKRAIRSIIDRTPAHLLKEIVLVDDYSSSEDLKGKLDDYIDLIHEKHPGLVKRVRHSEQRGLTQSRISGWEAATGEVVAILDAHIEVHAEWAEPLLARIKENRTVVVSPVFDRVNFDNLEVVKYTPAAHAFDWALWCMYESFQPDWYRLKDPSRPGKSPSLMGILVADRKFLGEIGSLDGGMKIYGGENVELGVRIWLCGGSIEIVPCSKIAHIERAHKPYLPDLSITMKRNALRVAEVWMDEYKKNINIAWNLPIKDHGIDIGDVSERKKLREKLKCKPFKWYIDNVYPQLDPWEDLVAYGALKNTVKENVCIDQGPVPGNVPIAFGCYYYGPQNTYYRKSGELYIGGIKSHKYNNNRCLADPGTGSTPGLYECSVAKQKGYSIYWDFEQGKAMINRKTHRCLEVEQGTLVIQNCTGQGWKIQHNVTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJ82</accession>
    <name>W5NJ82_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2'-deoxynucleoside 5'-phosphate N-hydrolase 1</fullName>
            <ecNumber evidence="6">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DNPH1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5-hydroxymethyl-dUMP + H2O = 5-hydroxymethyluracil + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:77099"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16964"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:90409"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:77100"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a purine 2'-deoxyribonucleoside 5'-phosphate + H2O = a purine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:51132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142198"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a pyrimidine 2'-deoxyribonucleoside 5'-phosphate + H2O = a pyrimidine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:57852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26432"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142209"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 6">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01001750">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJ82"/>
    <dbReference type="FunCoup" id="W5NJ82">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020691"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020727.1">
        <property type="protein sequence ID" value="ENSLOCP00000020691.1"/>
        <property type="gene ID" value="ENSLOCG00000016740.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2J2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001216"/>
    <dbReference type="HOGENOM" id="CLU_100069_1_0_1"/>
    <dbReference type="InParanoid" id="W5NJ82"/>
    <dbReference type="OMA" id="EVLSWHV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016740">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070694">
        <property type="term" value="F:5-hydroxymethyl-dUMP N-hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006195">
        <property type="term" value="P:purine nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000019">
        <property type="entry name" value="2'-deoxynucleoside 5'-phosphate N-hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03036">
        <property type="entry name" value="Nuc_phosphate_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051239">
        <property type="entry name" value="2'-dNMP_N-hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028607">
        <property type="entry name" value="DNPH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364:SF0">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="13"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="152" mass="16721" checksum="D899994AD3DCBD2A" modified="2014-04-16" version="1">FASFESSMALNIYFCGSIRGGRQDVAVYQKIVQKLQKYGTVLTEHVSHAALSEKVPGEDAVLEGDKAIHDRDVDWLKQADVIVAEVTQPSLGVGYELGRAVAMDKRVLCLFRPSSGRVLSAMIRGAADGSRFQVRDYAEEEAETTVEDYFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJY4</accession>
    <name>W5NJY4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin-conjugating enzyme E2 J1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">E2 ubiquitin-conjugating enzyme J1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Non-canonical ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis. Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the HRD1 complex, which comprises at least SYNV1/HRD1, DERL1/2, FAM8A1, HERPUD1/HERP, OS9, SEL1L and UBE2J1. Interacts with E3 ligase RNF26. Interacts with E3 ligase RNF133.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011947">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJY4"/>
    <dbReference type="FunCoup" id="W5NJY4">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020943"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020979.1">
        <property type="protein sequence ID" value="ENSLOCP00000020943.1"/>
        <property type="gene ID" value="ENSLOCG00000016942.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156652"/>
    <dbReference type="HOGENOM" id="CLU_041481_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJY4"/>
    <dbReference type="OMA" id="CGSTMKD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016942">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000043">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="19">
        <location>
            <begin position="9"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="184"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="320" mass="34877" checksum="7B456ED5B1F09E2A" modified="2014-04-16" version="1">TSFFFCAPVAVKRLMKEAAELRDPTEHYHAQPLEDNLFEWHFSVRGPPDSEFDGGVYHGRIVLPPDYPMKPPSIILLTSNGRFEVGKKICLSISSCHPSKFISLSVRTALIAIIGFMPTKGEGAIGSLDYTPEERKSLAKKSQDFHCEACGCTMRTALMPLTSNSNPSQEDKEAKELAKQINFKAESSTSSTDAQSPGAESSPTSGTQAQALSSGTQAAAAAALPEAEQKPCPSSPFQAAPQSDPTEEPPQPSRRLSPRQRRAQQPRRPASPAFAQIQRRPADAHHTGSAVLIVLLTLALAALIFRRIYLANEYKLDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKC0</accession>
    <name>W5NKC0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01026679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKC0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021079"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021115.1">
        <property type="protein sequence ID" value="ENSLOCP00000021079.1"/>
        <property type="gene ID" value="ENSLOCG00000017059.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169423"/>
    <dbReference type="HOGENOM" id="CLU_313289_0_0_1"/>
    <dbReference type="InParanoid" id="W5NKC0"/>
    <dbReference type="OMA" id="WHKTEAE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017059">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000015">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000033">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000018">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002800">
        <property type="entry name" value="Dynein heavy chain family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF15">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="2">
        <location>
            <begin position="298"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="3">
        <location>
            <begin position="442"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="4">
        <location>
            <begin position="634"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="680"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="935" mass="106420" checksum="BD670C61EA21C654" modified="2014-04-16" version="1">MEDLQTVEEKIAETLSQESSLLHHLALNRTLSDLHQHYDEALEMKSKLALLEKTALTAREGFRDLADRAALMFDTANTMRQVNPAYHISFSQLLEVFDSSAVHSERFSIKTLVDRLTYNIFCYIGRYMLERDQMVYTLLLAFEIESCLGHISPGMIEFVLSPDLCASLMEATKAKPSETRQAAKNPFEWMTEEQFKNVQILGTYYSWFHELFDRMFKDGKEMTWKALCDSDQPENPAKVKGPEGLSSLTALQRLLVVRAIRSDRLIQAASLFIGSTLGKKYITEIPSDLQRCIQQTTAQTPVLLLYSREAEEPRRLVLELCSQRSCKTEVVFVSEDSASEERRIKDVIENGQSKGSWVLLENIHNSMHLMTSIESMIHLNKNPNKQFRLWISAQANTSIPVRLMHVSLRIVVDTPKNMKDAMLRCLQQVGPDALRSCSRAEWPALIHNLCYLHCAVRLRAAAGGTTGWNFRHAMMFGIQEFRDALQVLREEFRDGSTSDVGEGRSVSWTGIRYLLTNVIYGKNITDDCDKRSLASLVDLWISSTATKKDNDMTKLKYRIPAAFFDPDAPLEGLLQALDSVPRHTLDVPEGTSLHSAPGLHFGDEQYACHWLKTLYKSLSFHHQQHSFAVQRHCRDFLLKSRKEEEVWEICCNIQAKIPKGWSKDFINERLKKIGGNTPFNLFLKKELNHLMELLSEVKRNLQAIRQSMEGPLRVGGTLSVTELSVAADLFRQRVPTHWSSLEGQDTPPEHWATATWVRDLQQRVLHFEKILQLGQEKMPTYWLGAFKNPKGLLGVFRQQSIQQHAEQTGFIEPMDLHTVITQRDKDHIRDPPQEGVFVYGLHVWGVSWHKTEAEILDAPPKHGPVALPVIHLRFLPKSEKAGLGDAARAADPYQCPVYGSSAGPRGPVLHLQVYKENIPAARWALRGMKATLQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL45</accession>
    <name>W5NL45_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase RNF217</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING finger protein 217</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the RBR family. RNF217 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01025944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL45"/>
    <dbReference type="FunCoup" id="W5NL45">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021354"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021391.1">
        <property type="protein sequence ID" value="ENSLOCP00000021354.1"/>
        <property type="gene ID" value="ENSLOCG00000017264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683982"/>
    <dbReference type="KEGG" id="loc:102683982"/>
    <dbReference type="CTD" id="154214"/>
    <dbReference type="eggNOG" id="KOG1815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111285"/>
    <dbReference type="HOGENOM" id="CLU_035717_1_0_1"/>
    <dbReference type="InParanoid" id="W5NL45"/>
    <dbReference type="OMA" id="QCSSCQF"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017264">
        <property type="expression patterns" value="Expressed in ovary and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20342">
        <property type="entry name" value="BRcat_RBR_RNF217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20350">
        <property type="entry name" value="Rcat_RBR_RNF217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000008">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000264">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047551">
        <property type="entry name" value="BRcat_RBR_RNF217"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047552">
        <property type="entry name" value="Rcat_RBR_RNF217"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="511"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="275"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="129"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="211"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="556" mass="61343" checksum="19702BCB9A6DEE99" modified="2014-04-16" version="1">MGSYTSGMEDEQISVNVADKEMRGFLTGLDPDVLGDLSEGVIDGEGIRDTDFASRERKVIKASDVLGSRKCRSEVGGSEDSSDRDTNRKPSAVDILRRNLGVPVPLANINNNSSDDQRDRVGSVVGVVLQRGTDSGDRGRSPGRENQARRVAGEEPGKAEDTPGFASPDPDEVGLARSEGHGLQGGHVYCTVYCVESGHCWGPPESLGARLSLGGGRTTPPVEPGVPAQPERDRPPQMDPEPDPRRSAAEPFTVSGVIDSRTSADPHAGDRGLSVVLTCRICLDDKHIEPLSCCRRAVCGDCLQRYLSSQVHLGRVGITCPITECSEHLEDSTVLAHLPGEDIAKYMYFLELSRVDSSTKPCPQCNHFTSLKSKTPSKSESRYKVQCPKCQFVWCFRCHAPWHDGLNCKDYRKGDKLLRHWASAIEHGQRNAQKCPRCKIHIQRTEGCDHMTCSQCNTNFCYRCGEKYRHLRFFGDHTSNLSVFGCKYRYLPEKPHLRRLIRGSVCVSKVLVAPVVIVLVVVFGAIALVIGLIAFPIYYICKKQRKRSQGTGRWLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLQ8</accession>
    <name>W5NLQ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLQ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021567"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021604.1">
        <property type="protein sequence ID" value="ENSLOCP00000021567.1"/>
        <property type="gene ID" value="ENSLOCG00000017462.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVH7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234640"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NLQ8"/>
    <dbReference type="OMA" id="MFICALS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF345">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="321" mass="36774" checksum="F01A01EAFD204A87" modified="2014-04-16" version="1">MQNSTLVTMFIFTTYEGIGHFRYLYFTATLLGYLLIFFLNIVLISVIIRERKLHEPMYIFICNLSLNGLYGSTAFYPNILTNLLSETPSISRAGCLTQVFFMHTYASFEYSVLALMAYDRYLSICHPLMYNSIMTPSKIIQLLVFVYMYPILIMLIHLILTIRLPLCGFIIEKVYCDNWSVVRLSCSDVSINSAFGLIVISLLICVPFLVVLYSYVRILAVCLKSTKEARAKALQTCTPHLLSFTNYSIAAFFEVIQHRVDLTNVPHILRILASIDCVLLPPVLNPIIYGVKLQEIRRRTMKMFFGRKGNHVEPPVGIKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM10</accession>
    <name>W5NM10_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zgc:154093</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069042464.1">
        <property type="nucleotide sequence ID" value="XM_069186363.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="W5NM10">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021669"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021706.1">
        <property type="protein sequence ID" value="ENSLOCP00000021669.1"/>
        <property type="gene ID" value="ENSLOCG00000017564.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689374"/>
    <dbReference type="KEGG" id="loc:102689374"/>
    <dbReference type="eggNOG" id="ENOG502S07T">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_073229_0_0_1"/>
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    <dbReference type="OrthoDB" id="9948028at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:cytoskeleton"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Cdc42_effect-like"/>
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        <property type="entry name" value="CDC42 EFFECTOR PROTEIN 2"/>
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            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
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            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
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            <end position="146"/>
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        <location>
            <begin position="166"/>
            <end position="223"/>
        </location>
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        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="252" mass="26906" checksum="F18D6E67575D5D07" modified="2014-04-16" version="1">MPAKTPMYLKTSTPKRGKKLKLRDILSGDMISPPLGDLRHSAHVGPEGETDMFGDVGFIQGKLDLLPGMSRARSRSAERGRAENPPADKRGQQPAGTAPTLLKNTVSLPVFSPEQAPPKPPRLHLEETPPAQRSMSVSCEGPRVVYSDPELSHSASIPAFGRLVASSGSFSEASSEDSVLGVGPPLDARRGLSLDSDAGLSNEDLQSERSESPGVPNSESLLGLDLDLGPSILEDVLRIMDQYKAQDGDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMP4</accession>
    <name>W5NMP4_LEPOC</name>
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        <submittedName>
            <fullName evidence="10">SRY-box transcription factor 1a</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021903"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021941.1">
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        <property type="gene ID" value="ENSLOCG00000017799.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG0527">
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_021123_0_0_1"/>
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    <dbReference type="OMA" id="MMETDMH"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="9">
        <location>
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            <end position="105"/>
        </location>
    </feature>
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    </feature>
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        <location>
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            <begin position="14"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="343" mass="36717" checksum="F8EE74CDA5104B1C" modified="2014-04-16" version="1">MYSMMMETDLHSPGPQTNANSGQTGPNSGNKSNQDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKVMSEAEKRPFIDEAKRLRAMHMKEHPDYKYRPRRKTKTLLKKDKYSLAGGLLAAGAGSGGGVGLGVGMSPAGVGQRLESPGSHGGTANAGYAHMNGWANGAYSGPVAAAAAAAAMMQEAQLAYSQHPGSGAHHHHAHHHHPHNPQPMHRYDMTALQYSPISNSQSYMSASPSGYGGISYTQHQNSSVASSGAIGTLGSLVKSEPSVSPPVTSHSRAPCPGDLREMISMYLPTGEAGDPSVQNRLHALPQHYQSTTTGVNGTVPLTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN63</accession>
    <name>W5NN63_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cyclin-dependent kinase 5 activator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterodimer composed of a catalytic subunit CDK5 and a regulatory subunit CDK5R1 (p25) and macromolecular complex composed of at least CDK5, CDK5R1 (p35) and CDK5RAP1 or CDK5RAP2 or CDK5RAP3. Only the heterodimer shows kinase activity. Interacts with EPHA4 and NGEF; may mediate the activation of NGEF by EPHA4. Interacts with RASGRF2. The complex p35/CDK5 interacts with CLOCK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer of a catalytic subunit and a regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Endomembrane system</location>
            <topology evidence="17">Lipid-anchor</topology>
            <orientation evidence="17">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the cyclin-dependent kinase 5 activator family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217736.1">
        <property type="nucleotide sequence ID" value="XM_015362250.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217737.1">
        <property type="nucleotide sequence ID" value="XM_015362251.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN63"/>
    <dbReference type="FunCoup" id="W5NN63">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022072"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022113.1">
        <property type="protein sequence ID" value="ENSLOCP00000022072.1"/>
        <property type="gene ID" value="ENSLOCG00000017971.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698774"/>
    <dbReference type="KEGG" id="loc:102698774"/>
    <dbReference type="CTD" id="436788"/>
    <dbReference type="eggNOG" id="KOG3932">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008812"/>
    <dbReference type="HOGENOM" id="CLU_034132_2_0_1"/>
    <dbReference type="InParanoid" id="W5NN63"/>
    <dbReference type="OMA" id="IKKTPHP"/>
    <dbReference type="OrthoDB" id="7676799at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017971">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016533">
        <property type="term" value="C:protein kinase 5 complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000025">
        <property type="entry name" value="Cyclin-dependent kinase 5 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004944">
        <property type="entry name" value="CDK5_activator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23401">
        <property type="entry name" value="CYCLIN DEPENDANT KINASE-5 ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23401:SF2">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5 ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03261">
        <property type="entry name" value="CDK5_activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009324">
        <property type="entry name" value="Cdk5_activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="303" mass="33662" checksum="9BEC2A027F672CB9" modified="2014-04-16" version="1">MGTVLSLSPSYRKAALFEDGPATVGHYTAVQNSKNAKDKGLKRHSIISVLPWKRIVAVSARKKGSKKVQPNSAYQNNVTHLNNENLKKSQSCANLSTFGQDSAAQVSASTTSANGASSIKKAPPANPTAAAGTPKRVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFVYMLCRDVVSSEVATEHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESSKEAFWDRCLSIINLMSAKMLQINSDPHYFTQVFADLKNESQKEEEKSRLLIRLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP65</accession>
    <name>W5NP65_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 158</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP65"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022424"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022465.1">
        <property type="protein sequence ID" value="ENSLOCP00000022424.1"/>
        <property type="gene ID" value="ENSLOCG00000018323.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYJ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009719"/>
    <dbReference type="HOGENOM" id="CLU_085099_0_0_1"/>
    <dbReference type="InParanoid" id="W5NP65"/>
    <dbReference type="OMA" id="FCCVDFA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018323">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038962">
        <property type="entry name" value="TMEM158"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38324">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38324:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867908" evidence="2">
        <location>
            <begin position="21"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="24808" checksum="BB8E9913D802A64E" modified="2014-04-16" version="1">SLLLVLALTAAAGLLRRCRCWSDEDLLLPPINSSTRFLANLEVDVRYSKRSVEGSEASSETALQPLCNISVQRLMPTSLVARWDRHLGFQCDVLIYTTNNHGRAFFSAAFSRAVSPVVIEHLGVAGGQQEFRLCVGCGLSRSRRFGQARSYPLQAGEPLGFCCLDFGLDELKGDHSWRLNRKPIESTLVACFMTLVIIVWSVAALIWPVPIIAGFLPNGMEQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPB5</accession>
    <name>W5NPB5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Cytochrome c oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="21"/>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="17">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion inner membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AB042861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_839840.2">
        <property type="nucleotide sequence ID" value="NC_004744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPB5"/>
    <dbReference type="FunCoup" id="W5NPB5">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000025126.1">
        <property type="protein sequence ID" value="ENSLOCP00000022474.1"/>
        <property type="gene ID" value="ENSLOCG00000020984.1"/>
    </dbReference>
    <dbReference type="GeneID" id="807196"/>
    <dbReference type="KEGG" id="loc:807196"/>
    <dbReference type="CTD" id="4513"/>
    <dbReference type="GeneTree" id="ENSGT00390000017410"/>
    <dbReference type="HOGENOM" id="CLU_036876_2_3_1"/>
    <dbReference type="InParanoid" id="W5NPB5"/>
    <dbReference type="OMA" id="WSYEYTD"/>
    <dbReference type="OrthoDB" id="539285at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000020984">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13912">
        <property type="entry name" value="CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.90:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034210">
        <property type="entry name" value="CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01166">
        <property type="entry name" value="CYCOXIDASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0186">Copper</keyword>
    <keyword evidence="11 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II transmembrane region profile" evidence="20">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="19">
        <location>
            <begin position="92"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="230" mass="26143" checksum="DBEEB6275646EFA4" modified="2014-04-16" version="1">MAHPSQLGFQDAASPVMEELLHFHDHALMIVFLISTLVLYIIVAMVSTKLTNKHILDSQEVEIVWTILPAVILIMIALPSLRILYLMDEINDPHLTIKAMGHQWYWSYELTDYEDLNFDSYMIPTSDLSPGQFRLLETDHRMVVPMESPIRMLISAEDVLHSWAVPSLGMKMDAVPGRLNQTTFIASRPGVYYGQCSEICGANHSFMPIVVEAVPLQHFENWSSLMLEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVI6</accession>
    <name>W5LVI6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="W5LVI6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000143"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000143.1">
        <property type="protein sequence ID" value="ENSLOCP00000000143.1"/>
        <property type="gene ID" value="ENSLOCG00000000133.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102693861"/>
    <dbReference type="eggNOG" id="KOG0598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160490"/>
    <dbReference type="HOGENOM" id="CLU_000288_53_1_1"/>
    <dbReference type="InParanoid" id="W5LVI6"/>
    <dbReference type="OrthoDB" id="8845271at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000133">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000160">
        <property type="entry name" value="Serine/threonine-protein kinase 32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF33">
        <property type="entry name" value="SERINE_THREONINE KINASE 32B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="135"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="180" mass="20133" checksum="8D71E1A5619E00B3" modified="2014-04-16" version="1">MFRAERVHYSSTWSKGMVSLLRKLLAKDPECRLSSVTDIQSSPYLADVNWDAVLEKAVAPGFVPNKGRLNCDPTFELEEMILESKPLHKKKKRLAKSKSRDATKESCPLPAHLQQCLETVRQEFIIFNREKLRKHGKSGQDSSSECDTSTPTPGKVQDGHTCNNNMLGPSDSPTICKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVL7</accession>
    <name>W5LVL7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras/Rap GTPase-activating protein SynGAP-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVL7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000174"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000174.1">
        <property type="protein sequence ID" value="ENSLOCP00000000174.1"/>
        <property type="gene ID" value="ENSLOCG00000000159.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:107076186"/>
    <dbReference type="eggNOG" id="KOG3508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158438"/>
    <dbReference type="HOGENOM" id="CLU_2262851_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVL7"/>
    <dbReference type="OMA" id="HHPEIEY"/>
    <dbReference type="OrthoDB" id="8954063at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000159">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras/Rap GTPase-activating protein SynGAP-like PH" evidence="2">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="103" mass="12103" checksum="4825BB93943A344F" modified="2014-04-16" version="1">MRERRSESHRNKLLRRTVSVPVEGRHHPEIEYARSRRKSIATGKQPSMETPPTAPPQPFRQSSFLSRRLKGSIKRAKSQPKLDRTSSFRHMILPRFRSADQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW52</accession>
    <name>W5LW52_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fish-egg lectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tectonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LW52"/>
    <dbReference type="FunCoup" id="W5LW52">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000000359.1">
        <property type="protein sequence ID" value="ENSLOCP00000000359.1"/>
        <property type="gene ID" value="ENSLOCG00000000326.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S0FI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047886"/>
    <dbReference type="HOGENOM" id="CLU_1102488_0_0_1"/>
    <dbReference type="InParanoid" id="W5LW52"/>
    <dbReference type="OMA" id="NSAGHIY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000326">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF3">
        <property type="entry name" value="FISH-EGG LECTIN-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19193">
        <property type="entry name" value="Tectonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865205" description="Fish-egg lectin" evidence="3">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27527" checksum="B834A570F733519A" modified="2014-04-16" version="1">WMQTSPVGLFLLSLSVAEALQCRAVPGSLKQIDAGAGRVCGVDNADNLVSYQGNSWVSLAMKGKHVSVGPAGLWSVSLASLVFKQLRGRWIQVQPGSLIQIDAGGDKFVVGVNAANSIFCLNRGPVLQYAGQGNIPWIPVMGSLKYYSCGPFGCWGVNRLDQIFVKLDVSGDACKGSDRWYPIQGFLSLMEVGSDGSVYGVNRNGVVFKRLGVDACNPFGRSWLTLKAAGVARHVSYDRGILWVVARDGQVQRCQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWI9</accession>
    <name>W5LWI9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWI9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000496"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000497.1">
        <property type="protein sequence ID" value="ENSLOCP00000000496.1"/>
        <property type="gene ID" value="ENSLOCG00000000456.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2764475_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWI9"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000456">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF11">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="70" mass="8161" checksum="50AC481E616CA8D1" modified="2014-04-16" version="1">MLSKRFSLQSFAMRKHYFNDPETGFFKKTKRKVVPKNPMTDTSMLTDMMKGNVTNVLPMIVIGGWINWAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX67</accession>
    <name>W5LX67_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ral GTPase-activating protein subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">p170</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Non-catalytic subunit of the heterodimeric RalGAP1 and RalGAP2 complexes which act as GTPase activators for the Ras-like small GTPases RALA and RALB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the heterodimeric RalGAP1 complex with RALGAPA1 and of the heterodimeric RalGAP2 complex with RALGAPA2. Heterodimerization is required for activity.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069035767.1">
        <property type="nucleotide sequence ID" value="XM_069179666.1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000724"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000727.1">
        <property type="protein sequence ID" value="ENSLOCP00000000724.1"/>
        <property type="gene ID" value="ENSLOCG00000000654.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692509"/>
    <dbReference type="eggNOG" id="KOG3652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00700000104550"/>
    <dbReference type="HOGENOM" id="CLU_005005_1_0_1"/>
    <dbReference type="InParanoid" id="W5LX67"/>
    <dbReference type="OMA" id="CWEECCV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000654">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032484">
        <property type="term" value="P:Ral protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
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        <property type="entry name" value="Ral GTPase-activating protein, beta subunit (non-catalytic)"/>
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    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RALGAPB"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
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    <dbReference type="InterPro" id="IPR046859">
        <property type="entry name" value="RGPA/RALGAPB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344:SF1">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="8">
        <location>
            <begin position="1153"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="356"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="719"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1314"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="724"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1314"/>
            <end position="1328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072207"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1499" mass="166962" checksum="DF67A08CFFAF5CDA" modified="2014-04-16" version="1">MYSEWRSLHLVVQSDQGHLSVLHSYPNAVGRDVANAVVRPLGLVLNTPSSAGAESVLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVAPRDTIPQPIIKEPNLYVQTILKHLHNLFLPRPEHYSPNHFRLCQQVLTAVQKLARESGNMARETWEVLLLFLLRINDTLLAPPTVGGGIAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVICALTSRLLRFTYGPSFPPFKVPDEDANLIPAEMDNDCVAQTWFRFLHMLSNPVDLSNPAIVSTTPKFQEQLLNVSGVPQEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPAPTTTNTTPPHSRRHRAVTVNKNTIKTTTGSTAHPSKVQHQTSTTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHNGMNRDNSMTAIATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYEAGRAEACGTLCRIFCSKKTGEEILPVYLSRFDMVLIQGLQISEFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKAYVNPTELRRSSINILLSMLPLPHHFGNVKSEVLLEGKYSNDDNSMQDKAVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSALLESIGTQSELVGVECDQTAVKSHSRNSSGISTASGGSSEATTPDSERPAQALLRDYALHTDTAAGLLVRSIHLVTQRLNSQWRPDMSISLAALELLAGLAKVKAGVESSDKKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTSDQEVRYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPSSPCSLLNEDTLIKYSRLSSTTRHNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTVLIRGMSGRHAWTMQLYHQPRGGRANQKVFVPESRPTPKNDVGVKFNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNKELEMQHDKLRNVMVKQMEYETAVERQSEDRWRNKTFPDPLIDCKPPAPSQEFQTARLFLSHFGFLSLEALKDPGNSRLPPHLIALDSALPGFFDDIGYLDLLPCRPFDTVFVFYMKAGQKSSHEILRNVDSSANVQCHFLEFLLSLGWPVDVGKHPGWTGNVYTSWSLNTGNGSERQNQEEAVTLEDTAGGVFNGEKKVLYYADALTEIAFVVPSLMDSSADSSENSFPSMESDSQMDLLPSLLKQSSLTLELFPNHSENLSSSQRLSPTVKSKKMPPGRTVPPLGPETKVFVVWVERYDDIENFPLSDLLADTSTGVEATMNSSTSLRPTMSEKDVPVIFIHPLKTGLFRIKLHGAVGKFSMVIPLVDGMVISRRALGFLVRQTVINVCRRKRLESDSYNPPHVRRKQKIAEIVNKYRNKQLEPEFYTSLFQEVGLKNLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX77</accession>
    <name>W5LX77_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 1 open reading frame 216</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009284">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LX77"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000737.1">
        <property type="protein sequence ID" value="ENSLOCP00000000734.1"/>
        <property type="gene ID" value="ENSLOCG00000000664.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001867"/>
    <dbReference type="HOGENOM" id="CLU_1067683_0_0_1"/>
    <dbReference type="InParanoid" id="W5LX77"/>
    <dbReference type="OMA" id="MLMGGWD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000664">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027812">
        <property type="entry name" value="DUF4653"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673:SF1">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15546">
        <property type="entry name" value="DUF4653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="296" mass="32023" checksum="18FA2AE28A404115" modified="2014-04-16" version="1">LSNQQPDRKCHPAAEKDRNFNFLSQEGTGGEEQRGETSVGATLGPLTRDENRNRLGPRLQNQCNQQARSSLSPPSSRLPCWSSLAPVLETREEGRRVPPDGAEGQDKPETERGAAGEEEEGEEDEEEEGEESGSEFSCRSSSSSSLSTESGGEGEGKGHAGDLGCVWERICGGHPKTDSPPSGPGDPAAALPRDRGGGEGSAPAVSASDSDSDPSQVLPGLLQAVWTLRDQERFKAKEKERHQVQLTMYRRLALIRWVRSLLGRVQDQQNRLQASYDIILTHRKELLRCGGAAAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYB1</accession>
    <name>W5LYB1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011662">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636378.1">
        <property type="nucleotide sequence ID" value="XM_006636315.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214054.1">
        <property type="nucleotide sequence ID" value="XM_015358568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYB1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001118"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001122.1">
        <property type="protein sequence ID" value="ENSLOCP00000001118.1"/>
        <property type="gene ID" value="ENSLOCG00000000995.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684614"/>
    <dbReference type="KEGG" id="loc:102684614"/>
    <dbReference type="eggNOG" id="ENOG502QQJ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002862"/>
    <dbReference type="HOGENOM" id="CLU_058117_1_0_1"/>
    <dbReference type="InParanoid" id="W5LYB1"/>
    <dbReference type="OMA" id="DCCERIG"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000995">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000118">
        <property type="entry name" value="Histamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016673">
        <property type="entry name" value="HHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016616">
        <property type="entry name" value="HHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51597">
        <property type="entry name" value="SAM_HNMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="291" mass="33242" checksum="F03E6BC409E69810" modified="2014-04-16" version="1">MTSAATDSSGYEGRYAQAFKLFLQRSTEHSSIFDFIDRTLLGEFTRIGDGKSTLNVLGLGSGGGEVDVHLLTKLQSVHLEKTITADIVEPSKELTQTFKALVAKTPNLKNIRFTWNTMTCAEYEQRVKETKDDKRFDFIHMIQMLYYVSDYAATIKFFHSLLREKGKLLIIHEAAGSGWELLWKTYKEALCTNKISQYLSAGDIKIHLENLGLKYEEHEIPNTLDITECFMKGNETGELLLDFMTEKNHFYKTTPPDLREGILELLRNKCSKEKDGRIIFNSNLSALFIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYE1</accession>
    <name>W5LYE1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ectonucleoside triphosphate diphosphohydrolase 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYE1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001148"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001152.1">
        <property type="protein sequence ID" value="ENSLOCP00000001148.1"/>
        <property type="gene ID" value="ENSLOCG00000001020.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693306"/>
    <dbReference type="KEGG" id="loc:102693306"/>
    <dbReference type="CTD" id="956"/>
    <dbReference type="eggNOG" id="KOG1386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286965"/>
    <dbReference type="HOGENOM" id="CLU_010246_2_3_1"/>
    <dbReference type="InParanoid" id="W5LYE1"/>
    <dbReference type="OMA" id="GNAISDM"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001020">
        <property type="expression patterns" value="Expressed in embryo and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000068">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF38">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="209"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="496" mass="55531" checksum="A5251B7E777129FC" modified="2014-04-16" version="1">MASKLAAALALLFLLTSLTVIVTVAIIQIHKTHYLSPGDKYGIVLDAGSSRTTVYVYKWPAEKENNTGVVKQIMICNIDGPGISSLGLNAEQDHRSWSSMEDCMNKTQQKIPPSQHNTTPIFLGATAGMRLLRSKNATAANAIMSEMERYLQRLPFRFQNASVISGEEEGLYGWITANYLMDNFLEKNIWNAWVRPHGAKTIGSLDLGGASTQIAFSPEEPDTSDRFRRVTLYGYEYSVYTHSYTCYGRNEAERQVLAALVQTSSGTSENPCYPQDYSAVIPEEYIFGSQCTAAKKPVNYNPKKHVTFVGTGDPALCRETVLSIFNLSSCEGEDHCSFNGVYQPALTGMFVAYAGFYYTTGALKLKGYFSLEDFNSTTWSFCSRKWKDLQSNYSSVKESYLRSYCFSANYIYNLLVYGYKFNSHTWEQIRFQKEVNNSNIAWSMGYMLILSNMVPAEARLVVLPLSNSVFAGLLFMFSALAILSLMFLIISVVRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYK4</accession>
    <name>W5LYK4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01024581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYK4"/>
    <dbReference type="FunCoup" id="W5LYK4">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001211"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001215.1">
        <property type="protein sequence ID" value="ENSLOCP00000001211.1"/>
        <property type="gene ID" value="ENSLOCG00000001061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698609"/>
    <dbReference type="KEGG" id="loc:102698609"/>
    <dbReference type="CTD" id="994"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158524"/>
    <dbReference type="HOGENOM" id="CLU_014464_0_1_1"/>
    <dbReference type="InParanoid" id="W5LYK4"/>
    <dbReference type="OMA" id="MHSKCRR"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001061">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000004">
        <property type="entry name" value="M-phase inducer phosphatase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF76">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06617">
        <property type="entry name" value="M-inducer_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="425"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="574" mass="64355" checksum="87644F01F7EADAA8" modified="2014-04-16" version="1">MMEYSEVFFSDFSPRVPQARGGALSLPGLGSRDSPCTRTLFSPEPPAVLSPVTNLALNMTHLAVLGSQCDTPKRRAPVPLLKIPSFASDASSDAGLGMDSPASPLDPAAVEERFEKALLESGAILRDDKIPIRRINSLPVRLLGSSPALKQKDPDGRSFGIFSRGGSESSGNRSGKENLHEGVFEFKKPAVPMSRCRLRSFHSTEAHDPSARRPSSAPALMLSPPPVARLCSLEDGSPVVLRRSSLTSSLDDDSDDGFLDVLDSDMESNAEMPLGIASLLTAPLVQEKEEQSLDSPIIRCRPRGLFRSPSLPASAGRAHLKRPECPRDEHTPVRVKRRRSVAGTVRPEDTEVEEGTPGMSCRLVQRSKSFCDQDIEKLLDSDVKELIGDFTKPFVLPTVEGKHQDLKYITSDMMMAALKGEFNHLVEQILVIDCRYPYEFEGGHIKGALNLHLEEEVEKHLLKTPIMPSSPDKRVLLIFHCEFSSERGPRMCRFVRERDRMMNEYPNLHYPELYVLKGGYKEFFPRYEAHCEPQGYRPMHHEDFREHLRKCRLRSRTWAGERSKRDLYSRLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYM2</accession>
    <name>W5LYM2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-activated potassium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">BK channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Calcium-activated potassium channel, subfamily M subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Charybdotoxin receptor subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">K(VCA)beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Maxi K channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Slo-beta-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022400">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022401">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYM2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001233.1">
        <property type="protein sequence ID" value="ENSLOCP00000001229.1"/>
        <property type="gene ID" value="ENSLOCG00000001089.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183039"/>
    <dbReference type="HOGENOM" id="CLU_085739_1_1_1"/>
    <dbReference type="InParanoid" id="W5LYM2"/>
    <dbReference type="OMA" id="ACYSDPE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001089">
        <property type="expression patterns" value="Expressed in heart and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.81.20:FF:000001">
        <property type="entry name" value="Calcium-activated potassium channel beta 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.81.20">
        <property type="entry name" value="KCNMB2, ball/chain domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003930">
        <property type="entry name" value="K_chnl_Ca-activ_BK_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037096">
        <property type="entry name" value="KCNMB2_ball/chain_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015382">
        <property type="entry name" value="KCNMB2_ball_chain_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258:SF5">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03185">
        <property type="entry name" value="CaKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09303">
        <property type="entry name" value="KcnmB2_inactiv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01450">
        <property type="entry name" value="BKCHANNELB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="KCNMB2 ball/chain" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00084024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF09303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="234" mass="26379" checksum="5F7522B414B28601" modified="2014-04-16" version="1">MFLWTGSRTAQGPGPERRTIYQKIREHDLLDKRKTVTALRAGEDRAMLLGISMMGFSVMTYFLLGITVVRSYTESIWTGESNCTVLNSSIVADVNCSYSCGSDCWKGSRYPCLQVFVMLNSSGKAARLSHNEEMQELNPECFFVPKCRKDYSVAQTIIRNISERLKVHQHFPCYYDPGEGQASVLLTRLHGRRAVLLSLLWPSCTLLGGALIVALVKLTQHLSILCEHINKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZB7</accession>
    <name>W5LZB7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hypocretin receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HCRTR1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZB7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001474"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001479.1">
        <property type="protein sequence ID" value="ENSLOCP00000001474.1"/>
        <property type="gene ID" value="ENSLOCG00000001293.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286969"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_3_1"/>
    <dbReference type="InParanoid" id="W5LZB7"/>
    <dbReference type="OMA" id="VENTSAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001293">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016499">
        <property type="term" value="F:orexin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15208">
        <property type="entry name" value="7tmA_OXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000204">
        <property type="entry name" value="Orexin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF32">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 15-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01064">
        <property type="entry name" value="OREXINR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="351"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="70"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="442" mass="49168" checksum="C8750E5B1CB31531" modified="2025-10-08" version="2">MTRGGMDRAQLNASAPGASEPNGTAQAGGAHSDYEEEILRYLWKEYLFPRQYEWVLIAGYIFVFVVALTGNILVCLAVWRNHHMRTVTNYFIVNLSLADLLVTAICLPVSLVVDITESWFFGQTLCKVIPYLQTVSVSVSVLTLSFIALDRWYAICHPLMFKSTARRARNSIVLIWLLSLAIMVPQAVVMETSSMIPELANRTLLLSVCEERWGGEVYPRVYHVCFFLVTYLAPLCLMFMAYFQIFRKLWSRQIPGASGAVSRKWVRGAGSSDDGGQAPGVERPSGSAGLVARPGTAPSAEVKQLRARRKTAKMLLVVLLVFSLCYLPISVLNVLKRVSGVFDNAGDREAIYAWFTFSHWLVYANSAANPIIYNFLSGKFRGEFKAAFSCCFRGLRRCRGAEARRLARTTSQKSLTNGSKSEPLSSRVSEHVVLSSVRAVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0M4</accession>
    <name>W5M0M4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-related protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SYS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0M4"/>
    <dbReference type="FunCoup" id="W5M0M4">
        <property type="interactions" value="1381"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001931"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001936.1">
        <property type="protein sequence ID" value="ENSLOCP00000001931.1"/>
        <property type="gene ID" value="ENSLOCG00000001677.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156407"/>
    <dbReference type="HOGENOM" id="CLU_040729_7_2_1"/>
    <dbReference type="InParanoid" id="W5M0M4"/>
    <dbReference type="OMA" id="NIERCVH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001677">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04160">
        <property type="entry name" value="Arfrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000509">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="204" mass="23063" checksum="E9A6B949A544346B" modified="2014-04-16" version="1">MYTLLSGLYKYMFQKDEYCILILGLDNAGKTTFLEQTKTKFSKNYKGMSLSKITTTVGLNIGTIDVGKARLMFWDLGGQDELQSLWDKYYAESHGVIYVIDSTDEERLSESKNAFEKMISSEALEGVPLLVLANKQDVEGCLSVPEIKTAFKSICCPLLIISTRTCLVDIYMELCSQGVNEGIEWMVKCVVRNIHRPPRQKDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M149</accession>
    <name>W5M149_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SRY-box transcription factor 8a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M149">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002106"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002111.1">
        <property type="protein sequence ID" value="ENSLOCP00000002106.1"/>
        <property type="gene ID" value="ENSLOCG00000001820.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159920"/>
    <dbReference type="HOGENOM" id="CLU_031800_0_0_1"/>
    <dbReference type="InParanoid" id="W5M149"/>
    <dbReference type="OMA" id="AQVWTHK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001820">
        <property type="expression patterns" value="Expressed in camera-type eye and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22031">
        <property type="entry name" value="HMG-box_SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000004">
        <property type="entry name" value="Transcription factor SOX-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022151">
        <property type="entry name" value="Sox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050917">
        <property type="entry name" value="SOX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45803">
        <property type="entry name" value="SOX100B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45803:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12444">
        <property type="entry name" value="Sox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="103"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="103"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="408" mass="45398" checksum="9648C4C246C182F0" modified="2014-04-16" version="1">FYSQMLKMTEEHDKSLADPPCSPAGTTSSMSQEDSDSDAPLSPTGSDGPGSQHRLGKKLEGEEDERFPACIRDAVSQVLKGYDWSLVPMPVRGSGSLKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLSESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKTGQSDSDSGAELSHHPSSQIYKSEPLGGLADGHHHSDHAGQPHGPPTPPTTPKTDLHQVSKQDLKHEGRRLLDSGRQNIDFSNVDITELSTDVIGNIEAFDVHEFDQYLPPNGHAXKTEQLSPSHYSEHSHGSPAPAEYSSYGAQTSAASPTVATSFSSSQCDYTDLQSSNYYNPYSGYPSSLYQYPYFHSSRRPYATPLLNSLSIPPSHSPTANWDQPVYTTLTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1D6</accession>
    <name>W5M1D6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5',3'-nucleotidase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1D6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002193"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002198.1">
        <property type="protein sequence ID" value="ENSLOCP00000002193.1"/>
        <property type="gene ID" value="ENSLOCG00000001889.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPWJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011596"/>
    <dbReference type="HOGENOM" id="CLU_100259_0_0_1"/>
    <dbReference type="InParanoid" id="W5M1D6"/>
    <dbReference type="OMA" id="VLFTSCH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001889">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:pyrimidine deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="HAD_5-3dNT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5'(3')-deoxyribonucleotidase, cytosolic type"/>
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    </dbReference>
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        <property type="entry name" value="Deoxyribonucleotidase, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="HAD_sf"/>
    </dbReference>
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        <property type="entry name" value="5'(3')-DEOXYRIBONUCLEOTIDASE"/>
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        <property type="entry name" value="5'(3')-DEOXYRIBONUCLEOTIDASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06941">
        <property type="entry name" value="NT5C"/>
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        <property type="entry name" value="C1.2.1"/>
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        <property type="entry name" value="C1.2:_Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="24849" checksum="63B593BEAC4454DA" modified="2014-04-16" version="1">LSSVTFSFKSERRPKHGKKRLRVLVDMDGVIADFEGGFLKKFRARYPNEPYITLEDRRGFWVSTQYGHLRSDLCEKAISIWETKNFFIDLEPLPGAVVAVKEMSKLENTDVFICTSPINNYNHCPYEKYAWIEKHLGCEFLEKVILTRDKTVVCADLLIDDKPDISGAEPHPAWEHVLFTACHNEHLPACRWRRRLHSWQDDWRALLDSKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1R3</accession>
    <name>W5M1R3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Solute carrier family 8 member 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01033839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01033848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1R3"/>
    <dbReference type="FunCoup" id="W5M1R3">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002321"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002326.1">
        <property type="protein sequence ID" value="ENSLOCP00000002321.1"/>
        <property type="gene ID" value="ENSLOCG00000001997.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164137"/>
    <dbReference type="HOGENOM" id="CLU_012872_1_0_1"/>
    <dbReference type="InParanoid" id="W5M1R3"/>
    <dbReference type="OMA" id="NCHEASG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001997">
        <property type="expression patterns" value="Expressed in camera-type eye and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000003">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000024">
        <property type="entry name" value="sodium/calcium exchanger 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF26">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER 4 ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calx-beta"/>
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    </dbReference>
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        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
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        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865499" evidence="22">
        <location>
            <begin position="39"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="770"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="801"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="847"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="919"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="23">
        <location>
            <begin position="407"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="23">
        <location>
            <begin position="536"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="946" mass="103696" checksum="39B546DE8995D004" modified="2014-04-16" version="1">MPGPCPSPPRPRPHPRLVLPPATLLPLLLLALTQLSGASEPPSAGASGTANCSGSSVCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTTTVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFHAGDLGPSTIVGSAAFNMFVIIAICVYVVPEGETRKIKHLRVFFVTAAWSIFAYIWLYLILAVVSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVQKRYRADKHRGIIIETEGDGAGGGGGPFTKMDLEMDGRAVNSHHKEALDGMLGPAEEGGGGRDQEDEARRDMARTLKELKQKHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEARADEDDLQTIRVQFEPSHYQCFENCGSLTLAVARRGGDDGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETLKELTVGIIDDDIFEEDEYFHVHLSNVRVVWPDGGPEPGAPPPPPKAALGAAHSATVTIFDDDHAGIFTFESEGTRVSESVGTMQVKVQRTSGARGTVVVPYRTVEGTAKGGEDYEEAAGKLEFLNDETMKVIEVKIIDDEEYEKNKTFTIELGEPVLVEIGQKHGDSNENRPAVGVEEEEVAKMGCPSLGEHTRLEVIIEESYEFKTTVYIISSAYRLSRCVSREGWRNRLGCRAALTMSCLPALPPGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFLVSISLIGVLTAVTGDLASHFGCTVGLKDSVTAVVFVVLTTPLPHTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWRAQGKDFRVDPGSLAFTVTLFTIFAFVNVTVLMYRRRASVAGGELGGPRTAKLLTTLLFLFLWLLYILLASLEAYCHIQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M261</accession>
    <name>W5M261_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA ligase</fullName>
            <ecNumber evidence="21">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1. Interacts with POLG. Interacts with POLB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="21"/>
    <dbReference type="EMBL" id="AHAT01005635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015223254.1">
        <property type="nucleotide sequence ID" value="XM_015367768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M261"/>
    <dbReference type="FunCoup" id="W5M261">
        <property type="interactions" value="1291"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002469"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002475.1">
        <property type="protein sequence ID" value="ENSLOCP00000002469.1"/>
        <property type="gene ID" value="ENSLOCG00000002087.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690708"/>
    <dbReference type="KEGG" id="loc:102690708"/>
    <dbReference type="CTD" id="3980"/>
    <dbReference type="eggNOG" id="KOG4437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156492"/>
    <dbReference type="InParanoid" id="W5M261"/>
    <dbReference type="OMA" id="GRWCTVT"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002087">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097681">
        <property type="term" value="P:double-strand break repair via alternative nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051053">
        <property type="term" value="P:negative regulation of DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18431">
        <property type="entry name" value="BRCT_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07967">
        <property type="entry name" value="OBF_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000085">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000032">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031916">
        <property type="entry name" value="LIG3_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16759">
        <property type="entry name" value="LIG3_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PARP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15 21" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="16 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="24" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865528" description="DNA ligase" evidence="24">
        <location>
            <begin position="29"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="domain" description="PARP-type" evidence="25">
        <location>
            <begin position="91"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="26">
        <location>
            <begin position="563"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="27">
        <location>
            <begin position="925"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="827"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="839"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="886"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00065554"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000617"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1001" mass="111475" checksum="9B221CAF9ABF66F7" modified="2014-04-16" version="1">MLKLCTHQTSRALFSTLLLKLFQNQASASESVQSFNFHRSATCAAYSSRSQAFLGKPWHACARTPASRAFHLSWSPCGGGGEREAMPEQRYCVEYAKRGTAGCKKCKEKIVKGLVRIGKIVPNPFSESAGDMKEWYHVKCMFEKLERARITTRRIEDITDLEGWEEVQDEDKELINKHIADLAAKTNHSPKKKVQTGLADLSPVTPRKFSGFTAAKPGTSSSPGGSSSLSAQLSDPSHKDCLLREFRKLCAMVAEKASYNVKTQIIQDFLKKGSSGDGFRGDLYLTVKLLLPGVIKSVYNLNDKQIVKLFSRIFNSSQDDMVRDLEQGDVSETVRIFFEASKSFPPAAKSLLTIQEVDGFLTRMSWLTREDEQQKALEEISKKSTANDLKCIIRLIKHDLKMNAGAKHVLDAVDPNAYDAFKASRNLADVIERVLRNQQDSANGSGPRKTLSVEASLMTPVQPMLAEACKSVEYAMKKCPNGMYSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKEYIPQAFPGGHSMILDAEVLLIDTKTSKPLPFGTLGVHKKAAFQDAKVCLFVFDCIYFNGVSLMERPLCERKKFLQDNMVEVPNRILLSEMKHVTKAADLADMITRVIREGLEGLVLKDLKSHYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAYYGKGSNGGMMSSFLMGCYDPESKQWCTVTKCSGGYDDATLARLQNELDVVKISKDPSKIPSWLKINKIYHPDFIARDPEKAPVWEITGAEFSKAEAHTADGISIRFPRCTRIRDDKDWQTATNLPQLRELFRISKERCDFELTAGLSKFSSDKGSSGGDSGSNSPSSTPRRPSTPSKPSTASSSSASKASCLPKTETPSPGKAVKRRLPADEHGGKKQKKAEAVHSNGESKLKTVSSQPAEPKQDKTLLDIFTGVKLYLPETVQDYGKLRRYFIAYDGDLVPKYDVATATHAIGDLEEESQAKRVSAEWIWKCIRKRRVVPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2B5</accession>
    <name>W5M2B5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01012300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012302">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2B5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002523"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002529.1">
        <property type="protein sequence ID" value="ENSLOCP00000002523.1"/>
        <property type="gene ID" value="ENSLOCG00000002150.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ46">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244939"/>
    <dbReference type="HOGENOM" id="CLU_013435_1_0_1"/>
    <dbReference type="InParanoid" id="W5M2B5"/>
    <dbReference type="OMA" id="GSWTINL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002150">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000090">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001406">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013808">
        <property type="entry name" value="Transglutaminase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF70">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00547">
        <property type="entry name" value="TRANSGLUTAMINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="9">
        <location>
            <begin position="161"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="596" mass="66627" checksum="4C622D3B34A0189D" modified="2014-04-16" version="1">IEVEVKLAPNAIVGKYSFKVKIGEDYTYETENGHFYVLFNPWCEDDDVHLPEENARQEYVLKDTGYIYTGSVNDITGKPWNFGQFERNVLDCCLYLLDKKGLKQSARREPVRVARAMSAVVNSNDENGVLYGNWSGNYADGTPPVYWTGSSAILQQYYESREPVRYGQCWVFSGVLTTVMRCLGIPSRSVTNFCSAHDTEGNLTIDIYLNQKGEKLNDITTDSVWNFHMWNDVWMKRPDLPEGYDGWQALDATPQEESEGIFQCGPSPLSAIKKGEVYLAYDTKFIFAEVNADRIIWLVENPKTDQQKTTRLRAHYSAVGKNISTKALGKDERQDITELYKFPEGTPEERKVMERASAVCFPDVPIPTPQSIQLGFESDKSFLLGSPITFQISLSNNSSQSQAVALTAACQLQTYNGKTLATIKALKQEVTVMANACTSVPVVIEASDYLQHLSMASDDLLVRVTAVGENKDSNSVFADDTTLSFKYPPITVDMPATAKTGEPFTCTFTFKNNIGVVMESCKLHVEGLGLFRLETFDQGNVRAGGIFRSKIICTPDRRGERKIIAKITSDQLDDINGYLVLTCNQKQCIIKTGYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2S5</accession>
    <name>W5M2S5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01011609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2S5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002683"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002689.1">
        <property type="protein sequence ID" value="ENSLOCP00000002683.1"/>
        <property type="gene ID" value="ENSLOCG00000002296.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163895"/>
    <dbReference type="HOGENOM" id="CLU_1222119_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2S5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002296">
        <property type="expression patterns" value="Expressed in liver and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26639" checksum="435D550B23C14460" modified="2014-04-16" version="1">PTREAEDEEKESFYNDLEAACDEAPRHDVKIILGDMNAKAGREPVYRLTIGKESLHEETNDNGFRLIRFAMSKGMTISSTWFPHKCIHKATWRSMNQIDQVLIKTGHGSDILDVRSYRGANIDSDHFLKNEWGVIKRTVLHASEEVVGCQVVNRREAWFDDECRVAIAERNEARKKMLQRPTRTSTAEFSSKRTLTKRLCRQRKTEWQNQKLQEYYNKEMRQVYQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3M7</accession>
    <name>W5M3M7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Y box binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015192550.1">
        <property type="nucleotide sequence ID" value="XM_015337064.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3M7"/>
    <dbReference type="SMR" id="W5M3M7"/>
    <dbReference type="FunCoup" id="W5M3M7">
        <property type="interactions" value="1386"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002985"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002991.1">
        <property type="protein sequence ID" value="ENSLOCP00000002985.1"/>
        <property type="gene ID" value="ENSLOCG00000002530.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695306"/>
    <dbReference type="CTD" id="4904"/>
    <dbReference type="eggNOG" id="KOG3070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153341"/>
    <dbReference type="HOGENOM" id="CLU_063071_1_0_1"/>
    <dbReference type="InParanoid" id="W5M3M7"/>
    <dbReference type="OMA" id="IIRFSHQ"/>
    <dbReference type="OrthoDB" id="203339at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002530">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000054">
        <property type="entry name" value="Nuclease-sensitive element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="38"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="34859" checksum="2156DBE88325310B" modified="2014-04-16" version="1">MSNEAETQQQPQQPAAAESPSSPAAAATAGDKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNRYRRYPRRRGPPRNYQQNYQSDGEGEGGEKREGGENVPEGDVQAQQRRPSFPRRRYPPYFVRRRYGRRPPYTNTPPRGEMTEGGEGDEIQGGADQGNKPMRQNYYRGYRPRGPPRPRPPREGSGEEDKENQGDGSQGQEARPRRYRRNFNYRRRRPQNPKPQDGKENKAAEPPAEKTSAPEAEQGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3R7</accession>
    <name>W5M3R7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Voltage-dependent L-type calcium channel subunit beta-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcium channel voltage-dependent subunit beta 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. Interacts with FASLG. Interacts with CBARP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636315.1">
        <property type="nucleotide sequence ID" value="XM_006636252.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3R7"/>
    <dbReference type="FunCoup" id="W5M3R7">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003025"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003031.1">
        <property type="protein sequence ID" value="ENSLOCP00000003025.1"/>
        <property type="gene ID" value="ENSLOCG00000002565.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689881"/>
    <dbReference type="KEGG" id="loc:102689881"/>
    <dbReference type="CTD" id="562422"/>
    <dbReference type="eggNOG" id="KOG3812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182837"/>
    <dbReference type="HOGENOM" id="CLU_021995_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3R7"/>
    <dbReference type="OMA" id="WRSTHAS"/>
    <dbReference type="OrthoDB" id="5962384at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002565">
        <property type="expression patterns" value="Expressed in heart and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-1 isoform 1"/>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CAB1-4_N_A-dom"/>
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        <property type="entry name" value="GK/Ca_channel_bsu"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
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        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="92"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
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        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="433"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="63"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="442"/>
            <end position="466"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002749"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00010836"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00064941"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00073550"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="519" mass="58277" checksum="9B3F52B05C2F6518" modified="2014-04-16" version="1">MSRHLYARHKLGDGLQTPIFQVTRGTQSRRSRLKRSDGSTTSTSFILRQGSADSYTSRPSDSDVSLEEDREGVRQEREQQAAIQLERAKAKAVAFAVKTNVSYCGALDEDVPVAGTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLKLENIRIQQEQKRGRFHGKSSGNSSSSLGEMVSGTFRNTPPSAAKQKQKVAEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWRATHVPNSTPMNPLLGRNLGSTALSPYPTAISGLQGQRMRHGNHSGEHSPIERRSLMTSDENYHSERARKSRNRLSSGSQHSRDHYPLVEEEYSDPYQDTYKPHRNRGSPGGYSHDSRHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3U0</accession>
    <name>W5M3U0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NADH-ubiquinone oxidoreductase 24 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone. Contains one iron-sulfur cluster.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29092"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004199">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3U0"/>
    <dbReference type="FunCoup" id="W5M3U0">
        <property type="interactions" value="1393"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003048"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003054.1">
        <property type="protein sequence ID" value="ENSLOCP00000003048.1"/>
        <property type="gene ID" value="ENSLOCG00000002593.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017580"/>
    <dbReference type="HOGENOM" id="CLU_054362_3_0_1"/>
    <dbReference type="InParanoid" id="W5M3U0"/>
    <dbReference type="OMA" id="THIRCLH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002593">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NuoE-like"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="139" mass="15503" checksum="E1D1E1CF55C6666F" modified="2014-04-16" version="1">TRQIRSLHKTAVRSGGGGALFVHRDTPENNPDTPFEFTPENLKRAEAIVQNYPDGHQAAATLPVLDLAQRQHGWLPISAMNKVAELLDVPPMRVYEVATFYTMFNRKPVGKYHIQICTTTPCMLCNSDSILEAIQNKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5M442</accession>
    <name>W5M442_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M442"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003157.1">
        <property type="protein sequence ID" value="ENSLOCP00000003150.1"/>
        <property type="gene ID" value="ENSLOCG00000002686.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT4S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00870000136917"/>
    <dbReference type="HOGENOM" id="CLU_1247893_0_0_1"/>
    <dbReference type="InParanoid" id="W5M442"/>
    <dbReference type="OMA" id="ECHRGRE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002686">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="198" mass="21166" checksum="DA8694228D49AD3C" modified="2014-04-16" version="1">AAAAAARPRAWSVRAFHDLLPPLPQLHRKWCSWNSTSPFTKLLDGGSAGRAPAAATSETECHRGREGKVLSEAHLENRAGRNLSDSSVSSASCPLSQPLQKQRRLSSTSTLRRARFASPCFGLLAGPSESSRGPAGPLIPYAQPQNSLTEMASGSSGPPLQADAQAKLRDFPSEEDHTSVPVFAISEEEDRQPLVLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4E4</accession>
    <name>W5M4E4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">AFG2 AAA ATPase homolog A</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the AAA ATPase family. AFG2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4E4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003252"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003259.1">
        <property type="protein sequence ID" value="ENSLOCP00000003252.1"/>
        <property type="gene ID" value="ENSLOCG00000002749.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698334"/>
    <dbReference type="KEGG" id="loc:102698334"/>
    <dbReference type="CTD" id="166378"/>
    <dbReference type="eggNOG" id="KOG0730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157323"/>
    <dbReference type="InParanoid" id="W5M4E4"/>
    <dbReference type="OMA" id="WPISEQF"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002749">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000567">
        <property type="entry name" value="ATPase, AAA family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000069">
        <property type="entry name" value="spermatogenesis-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000015">
        <property type="entry name" value="spermatogenesis-associated protein 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="12">
        <location>
            <begin position="50"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="382"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="656"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="892" mass="97196" checksum="58E52315107914F3" modified="2014-04-16" version="1">MSSKKKNKAKSGRSADGENSSVLGDSCSLDSSKVLTSNTEEERRVLSNLSFIVTDFIDKADEKVPKSCRRSLVQLSLNAMKSANICIGRPVLLSSSLSKQEVCTAWPTASFPGGKVGLHKWTQQNLKVKPGDVITVQPVTGPILQAEEIHLSFRQRDDAFNMEEFTNFLLRSMDGKVLLPGNFLGLTFYGRPCSLRVEKVKGVDGIRMETSGFPVTETEKEASGDSTLETTSVDLSLQLSHLNLEENEARPPASTPCKPHLCVSVTSDLEPCPSPVRESGEPCGSEDPSFSHSTSNPVHPGSSSPLSDCGVKCNSDTFYHLSLLTKIKLLEQKAHSKSELESTAKVTYSMIGGLSSQLSTIRETIELPLKHPDLFKRYGIPAPRGVLLYGPPGTGKTMIGRAIANEVGVHITVINGPEIMSKFYGETEARLRQIFSDASQRQPAIIFIDELDALCPKREGAQNEVEKRVVASLLTLMDGIGSEEHSGQLLVLGATNRPHALDPALRRPGRFDKEIEIGIPNAVERADILKKQLSTTSSLLSEEEIVMLADSAHGYVGADLAAVCKEAGLHALRRALSKDTQLSDAEVMGTVAVTFLDFQQAMNDVQPSAMREVAIDVPKVRWADVGGMDQVKLKLKQAVEWPLKHPESFSRMGINPPKGILLYGPPGCSKTMIAKALANESGINFLAIKGPELLSKYVGESERAVREVFRKARAVAPSIVFFDEIDALAVERGSSSGSSGVADRVLAQLLTEIDGIEQLRDVTVLAATNRPDMIDKALMRPGRLDRIIYVPLPDAATRREILKLQFSNIPVAEDVCLNDLVIQTKKYSGAEITALCQEAALLALQEDINAKYIRGKHFETALEIVKPRIPDSLIQSYVNYQKQSGLYTASHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4V7</accession>
    <name>W5M4V7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heat shock protein beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock 27 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641050.1">
        <property type="nucleotide sequence ID" value="XM_006640987.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4V7"/>
    <dbReference type="SMR" id="W5M4V7"/>
    <dbReference type="FunCoup" id="W5M4V7">
        <property type="interactions" value="748"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003415"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003422.1">
        <property type="protein sequence ID" value="ENSLOCP00000003415.1"/>
        <property type="gene ID" value="ENSLOCG00000002907.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688129"/>
    <dbReference type="KEGG" id="loc:102688129"/>
    <dbReference type="CTD" id="3315"/>
    <dbReference type="eggNOG" id="KOG3591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155882"/>
    <dbReference type="HOGENOM" id="CLU_095001_0_0_1"/>
    <dbReference type="InParanoid" id="W5M4V7"/>
    <dbReference type="OMA" id="EITGKHK"/>
    <dbReference type="OrthoDB" id="10060792at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002907">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014881">
        <property type="term" value="P:regulation of myofibril size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06475">
        <property type="entry name" value="ACD_HspB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000024">
        <property type="entry name" value="heat shock protein beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037876">
        <property type="entry name" value="ACD_HspB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF7">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="13">
        <location>
            <begin position="86"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="207" mass="22985" checksum="20E7857C56DEA582" modified="2014-04-16" version="1">MSERHIPFTFLRGPSWDPFRDWFHGNRIFDQSFGMPALTEDWYHWPSTHWPGYIRPSVVGPAAEAGAPSAVSPLPPGPAMSPYARALSRQLSSGMSEIKQTQDQWKVSLDVNHFSPEELVVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDVNKVVSSLSPEGVLTVEAPLPKPAIQAAEITIPVSLESKAKPAVETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5I1</accession>
    <name>W5M5I1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dipeptidyl peptidase like 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011569">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5I1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003639"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003646.1">
        <property type="protein sequence ID" value="ENSLOCP00000003639.1"/>
        <property type="gene ID" value="ENSLOCG00000003049.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154657"/>
    <dbReference type="HOGENOM" id="CLU_006105_4_1_1"/>
    <dbReference type="InParanoid" id="W5M5I1"/>
    <dbReference type="OMA" id="NFTQRYL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003049">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF21">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865585" description="Dipeptidyl peptidase like 10" evidence="3">
        <location>
            <begin position="29"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="5">
        <location>
            <begin position="94"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="4">
        <location>
            <begin position="547"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="86606" checksum="D60D441816454768" modified="2014-04-16" version="1">ILGVRCISLCFNCLLLIFLDYGLNSVSSSLTLDDLFRKELTVHDPEAKWINDEEIVYRDRDGNVMKHNVHSNQTGIVLKNTTFSMFKASTFAVSPDQNFVLLGYSVKQVYKYSFTASYSVYDLHTRNSREIVPLNPPEVPDSALQYASWGIQKQQLVYIFENNIYYQRDRQSNSLRLTSSGREGVVFNGIADWLYEVELLQSHVAHWWSPDGEKLAFLMINDSLVPNMALPRLTGSLYPRGQQYPYPKAGQVNPTVKLYVVNVNVVTQTVEMVPPSSFRKSDYYISMVKWTDNYSISVRWLNRPQNTSILTVCEASSGECVKKHVMTSDIWLDRQNEQPVFSKDGSKFFLTIPIKHGARGDFHHIAMFSDQSRSEEITIRHLTSGNWEVTEILAFDETQKIVYFLSTEDSSAQRQLYRVSVEEPFNRRCLTCGLYNGTCTFVSAEFSPTMQSAILNCKGPDIPKVSVHWLNNLGGFHILESNTEIREFMSGKKTLEKEVRILQVNNHELPMELTLPAGFSEKNLHPLLLIVDGAPGSQSVNDKFLLDWDSVLVSSDGVIVARFDGRGSGFQGQKILQQVHQRLGTLEVQDQKAAIEALIKLPYIDQRRIGVFGKAYGGFLTSLLLLSYDTIFRCGVAVAPVTDWRLYASAFTERYLGFPSENGRIYQACGILHNMTGSTHHKFLIIHGTADSNVHFQHTAELVKRLAQSNVNYMLKIYPDEGHDITSSKSRYYHYRTVMTFFRECFYEDSQIIPEQEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6V4</accession>
    <name>W5M6V4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ORAI calcium release-activated calcium modulator 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035885">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035886">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035887">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004112"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004120.1">
        <property type="protein sequence ID" value="ENSLOCP00000004112.1"/>
        <property type="gene ID" value="ENSLOCG00000003466.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015354"/>
    <dbReference type="HOGENOM" id="CLU_062509_1_1_1"/>
    <dbReference type="InParanoid" id="W5M6V4"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003466">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.140:FF:000001">
        <property type="entry name" value="Calcium release-activated calcium modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501:SF6">
        <property type="entry name" value="PROTEIN ORAI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="297" mass="31439" checksum="F7F4903E66635772" modified="2014-04-16" version="1">CSPAPAMQPEAGPGGSTAPSPAAIGEPPSDRKGGSGAAPDSDPAGALSSYQDFITSTYIDLVSANQHSVQALNWRRLYLSRAKLKASGRTSALLSGFAMVAMVEVSLQDDYPYPPGLLVSFSVVTTVLVALHLFALLVSTCILPHLEAVSNIHNLRCVAQSPHLVLRPYIELAWGFSNALGLLLFLAEVALLCWVKFLPLGGRPQTADSLRSNSSEAAVEGGRSSEGYGAALVATVVMVPAGLLFALFALHFYRALLTHKSQRHSQELLCLSTLQQQLDTXSWTRSEHPAGTAGHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7C5</accession>
    <name>W5M7C5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin-like protein 26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Ligand for integrins alpha-E/beta-7, ITGAE:ITGAB7, alpha-4/beta-7, ITGA4:ITGAB7 and alpha-4/beta-1, ITGA4:ITGAB1 through which modulates CD4(+) T cells activation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7C5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004283"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004291.1">
        <property type="protein sequence ID" value="ENSLOCP00000004283.1"/>
        <property type="gene ID" value="ENSLOCG00000003607.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691961"/>
    <dbReference type="KEGG" id="loc:102691961"/>
    <dbReference type="CTD" id="571792"/>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161589"/>
    <dbReference type="InParanoid" id="W5M7C5"/>
    <dbReference type="OMA" id="MRSGSHP"/>
    <dbReference type="OrthoDB" id="9045962at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003607">
        <property type="expression patterns" value="Expressed in zone of skin and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000095">
        <property type="entry name" value="Cadherin 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000158">
        <property type="entry name" value="Dachsous cadherin-related 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF78">
        <property type="entry name" value="CADHERIN-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01820">
        <property type="entry name" value="DESMOCOLLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 17 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 18" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865799" description="Cadherin-like protein 26" evidence="20">
        <location>
            <begin position="28"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="60"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="167"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="279"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="399"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="522"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="856" mass="95424" checksum="6D5DA99C1A82C0C3" modified="2014-04-16" version="1">MRGSIVFWMAKCTTLLLLTMAVLSCAGQEQNAEDVQDVGQTSLLKREKRELLRRSKRRWVLTTIEVTEQDEGPFPKHAATLFNDQVVNYQLKYVISGRGVTEHPIGIFSVNETDGRVFVHKPIDREIYPEFHVKFDVLDRATNRVVDRTLAFDVEVKDVNDNAPEFTIPVQNITVKETTNEGDLQLNIEANDIDLHGSPNSEFNFKMISQTPSYPAFSIRRITGTNKAIISFKGCFDYEKEKQYRILVEAKDKGEPTLSSTATININIEDGNNHPPVFTKQKYEAEVHEGDVNVVILRMAVKDEDNPNTPASNAVFKILSGNDNGNFQIETNKTTNEGILSVIKAKDYEEASLKNLVIAVENEEPLFTCPGTASHRNLYGPQTVNVSVAVKNINDAPVFQGLKPVYVSEAAKPGTVLTTLVAKDDETHLSKMRYELVSDPANWVAVNEQTGQITNIKEMDRESPFVNNSIYAIVVHAIDDGDPPATTTATVLIHLSDVNDNKPYLITKNVHICGDLDQNSVTVTAGDKDIEPYSGPFAYELLDKGQDVKTKWKLDQTSGDSVDLRSLQRLPNGNYSVPLKIEDKQGQSAEENLYVQVCDCEGMNHCRPLKAKSSALGGAAIGILLSSLLLLLLLLCLCLLCSSGRETLKKIPIYGQDEGNQTLIKYNEEGGGSMSQTGQTIIMNVMGSNGATDGVKQATLPLHAQIRPWSGMRSDETDGTMYRNGINSGTLLHEIPTRSQSRQSLWNSRMNINEDGTLKYSRSNSMRLHGFDAAQNELQEHITRRLYQLRDIEDDVTQNLPHIYAYEGQDSRHQSLDRLSIANSSNSLNFLHNLGPRFNTLDRICHQTMQEKGMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7I7</accession>
    <name>W5M7I7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1</fullName>
            <ecNumber evidence="18">2.4.3.2</ecNumber>
            <ecNumber evidence="19">2.4.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Gal-NAc6S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Monosialoganglioside sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">ST3Gal I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">ST3Gal II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">ST3GalA.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="36">ST3GalA.2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">ST3O</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Sialyltransferase 4A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="37">Sialyltransferase 4B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="42" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="41" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:139596"/>
            <dbReference type="EC" id="2.4.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:21617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a ganglioside GA1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27938"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:47561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>a ganglioside GA1 + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:88069"/>
            <dbReference type="ChEBI" id="CHEBI:90151"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:48245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>a ganglioside GM1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1a (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77709"/>
            <dbReference type="ChEBI" id="CHEBI:78445"/>
            <dbReference type="EC" id="2.4.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:18022"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>a globoside GalGb4Cer + CMP-N-acetyl-beta-neuraminate = a globoside MSGG + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:140623"/>
            <dbReference type="ChEBI" id="CHEBI:140691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:65373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>ganglioside GM1 (d18:1(4E)/18:0) + CMP-N-acetyl-beta-neuraminate = ganglioside GD1a (18:1(4E)/18:0) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73110"/>
            <dbReference type="ChEBI" id="CHEBI:90153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:48249"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="33">Homodimer; disulfide-linked. Homodimer formation occurs in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.2" evidence="18"/>
    <dbReference type="EC" id="2.4.3.4" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01012143">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7I7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004345"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004353.1">
        <property type="protein sequence ID" value="ENSLOCP00000004345.1"/>
        <property type="gene ID" value="ENSLOCG00000003647.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154725"/>
    <dbReference type="InParanoid" id="W5M7I7"/>
    <dbReference type="OMA" id="RPCSCHT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003647">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047288">
        <property type="term" value="F:beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003836">
        <property type="term" value="F:beta-galactoside (CMP) alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23966">
        <property type="entry name" value="GT29_ST3GAL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000002">
        <property type="entry name" value="CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051757">
        <property type="entry name" value="Beta-gal_alpha2-3_sialyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032">
        <property type="entry name" value="ALPHA-2,3-SIALYLTRANSFERASE ST3GAL I ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032:SF6">
        <property type="entry name" value="CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 40" id="KW-0472">Membrane</keyword>
    <keyword evidence="42" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 40" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 40" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="38">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="39">
        <location>
            <begin position="138"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00042990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00042991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00043673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00043773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00043816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00062545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00072809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00081228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00081332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00082805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="336" mass="38458" checksum="205EEE2BE71D323B" modified="2014-04-16" version="1">TFLLTAGMLRTLTLLFIILTLTTFLLSYTYHDPSTTYFYLLKLSGGATENLPKRVCACKRCISEPDIVPWFDERFNQSIHPLMSKKNSILSEDTYKWWQRLQSENNPANYTEVVQRLFEVIPGEEMYVDWGSSRCRTCAVVGNSGNLKGSNYGAQIDSHAFIIRMNHAPTFGYEKDVGSRTTHHLIYPESAKNLPNSTMLVLIPFKTLDLQWIISALTTGTIKHTYMPVLSKINVKNDKVLIYSPTFFKYVFENWLENHGRYPSTGFLSIMFAVHLCDEVNVFGFGADEKGNWHHYWENNPLAGAFRQSGVHDGDFEYNITILLSETGKINIFKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7M3</accession>
    <name>W5M7M3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elongin-C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Elongin 15 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase II transcription factor SIII subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">SIII p15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor B polypeptide 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (CUL2 or CUL5), a catalytic subunit (RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL, WSB1, ZYG11B or RAB40C. Interacts with TMF1. As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1. May form oligomers as a KLHDC2/KLHDC3-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2/KLHDC3 is blocked in the oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7M3"/>
    <dbReference type="FunCoup" id="W5M7M3">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004381"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004389.1">
        <property type="protein sequence ID" value="ENSLOCP00000004381.1"/>
        <property type="gene ID" value="ENSLOCG00000003689.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687026"/>
    <dbReference type="KEGG" id="loc:102687026"/>
    <dbReference type="CTD" id="447935"/>
    <dbReference type="eggNOG" id="KOG3473">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011717"/>
    <dbReference type="HOGENOM" id="CLU_130038_3_1_1"/>
    <dbReference type="InParanoid" id="W5M7M3"/>
    <dbReference type="OMA" id="NCEGPDS"/>
    <dbReference type="OrthoDB" id="249087at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003689">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18321">
        <property type="entry name" value="BTB_POZ_EloC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000016">
        <property type="entry name" value="Transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039948">
        <property type="entry name" value="ELC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20648">
        <property type="entry name" value="ELONGIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="16">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="90" mass="10084" checksum="C8844E84AA807E88" modified="2014-04-16" version="1">SSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8P6</accession>
    <name>W5M8P6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTPase-activating Rap/Ran-GAP domain-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the GARNL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025365">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025366">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8P6"/>
    <dbReference type="FunCoup" id="W5M8P6">
        <property type="interactions" value="459"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004754"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004762.1">
        <property type="protein sequence ID" value="ENSLOCP00000004754.1"/>
        <property type="gene ID" value="ENSLOCG00000003976.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3686">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159362"/>
    <dbReference type="HOGENOM" id="CLU_008685_1_0_1"/>
    <dbReference type="InParanoid" id="W5M8P6"/>
    <dbReference type="OMA" id="FEGYSER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003976">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000006">
        <property type="entry name" value="GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF62">
        <property type="entry name" value="GTPASE-ACTIVATING RAP_RAN-GAP DOMAIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="5">
        <location>
            <begin position="187"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="488"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="819"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="939"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="819"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00060925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00069072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1032" mass="116085" checksum="547F8EF6BBC9468E" modified="2014-04-16" version="1">MSSADPASTKLLSFTQRNKYSSASEDLGCRRGEFSRKHYGSVELLISSDADGAIQRAGRFRVENGSSDENTDYTPGTWRRTDVHLENPEYHTRWYFKYFLGKVHQNYVGTDADKNPFYLSVVLSDQNNQRVPQYRAILWRKAGTLKICLPYSPTKTLSVKSILSAMNLDRFEKGPREILNPEIQKDLLVLEEQEGSVNFKFGVLYAKDGQLTDDEMFSNESGSESFEKFLNLLGDTITLRGWAGYRGGLDTKNDTTGINSIYTVYQGHELMFHVSTMLPYSKENKQQVERKRHIGNDIVTIVFQEGDDMSPSFKPSMIRSHFTQTIDIFALVRYNSQNDSYRLKIFSEESVPLFGPPLPSPPVFTDHQEFRDFLLVKLINGEKATLETPTFAQKRQRTLDMLIRSLYQDLIPDLHKNMLNRRSFSDVLPESPKSARKKEEARQAEFVRIGQALKLKTIVRGDAPTSLVTTGLCRKEPWESQSFCSNFSYEIVCSDSWGQSLLIATDAGVVLLDAPDPCNLSMDSQPSPVQVFDKTMLVKQMHVLEAQDLLITRTDKGKDARLFVFRLGAIKKGLEDRQVRSKYDSRENKLEKTKGCHLYSINTHHSSELRIVAAIRNKLLLITRKHHRPDSLTNISMVTAPTESPVEEFQYIREICLCDPPVVMALVDGPTGEYDNMICVAYKHQFDLINESTGEAYRLHHVDANRVNFVAAIDVYEDGEAGLLLCYNYICYYKKVCPFNGATPMIQSSASDFHFSWNQVPNSIVCAFPYILAFTTDSIEIRLVVNGNLVHTAVVPELQLAASRSDIYFVSSAAVNAASNSSSKDTSSHSSPQTPTGYEMPTFPSPLGDDSIRIPYGTKLSLYMSKEAEGEVQLKHIYKIPLSNLVGRSIERPLKSPLVNKVVTTPTSGIVSSSIVPVTHSLSLSRMEIKEIASRTRKELLGLTDEPSNKSEGPQKQRRMSRKPPEEETKQGAVRSTSTDKIVSESFDTDSESQRHCSTSSDTDVVGVREESPPLTSPFTLATSFEDVVDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M920</accession>
    <name>W5M920_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TBCC domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011430">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M920"/>
    <dbReference type="FunCoup" id="W5M920">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004879"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004887.1">
        <property type="protein sequence ID" value="ENSLOCP00000004879.1"/>
        <property type="gene ID" value="ENSLOCG00000004084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696658"/>
    <dbReference type="KEGG" id="loc:102696658"/>
    <dbReference type="CTD" id="55171"/>
    <dbReference type="eggNOG" id="KOG4416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00470000042284"/>
    <dbReference type="HOGENOM" id="CLU_016712_1_1_1"/>
    <dbReference type="InParanoid" id="W5M920"/>
    <dbReference type="OMA" id="VPNAWDQ"/>
    <dbReference type="OrthoDB" id="427777at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004084">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031616">
        <property type="term" value="C:spindle pole centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051661">
        <property type="term" value="P:maintenance of centrosome location"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051684">
        <property type="term" value="P:maintenance of Golgi location"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039589">
        <property type="entry name" value="TBCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052:SF0">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="8">
        <location>
            <begin position="306"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="560" mass="62479" checksum="B1E7E57F4043F002" modified="2014-04-16" version="1">MEQRSVSVWPRLEPFLLGVLPAAPPAKFSMHYLRKMAAYVRARTREGCFPRLHWPLWRHIACGKLQLPEDTAWLYFETCDLLAPRPSEERLEWAEAVSQCGSVEELDKLRYKLSVDTLQFLLFLYIQQLNRVSLRTSLIGEEWPSPRSRSRSPSPDRDAKTAAQNKNWDDQAHLSFIQTHLSDLLELVLEPEQLGGPGQAVHDSQVSAEALRGLSFLLEGSLSRGRAVHGLHELLGWGPLQAQAGYSALTHSFSLPRLQAWLRESLTANPFGTTACLRSGKKLAWAQQVDGAMKRAKIARNTHIAPPGSRVVLMSQVYRQTVAKDSEKLAGANVKLHRCSEAYIYLLTPLRSVSVEKCRNTTVVLGPVQSTVHVQSCEDVCVVCVAGRLSVGASSRCTFHALTPTRPLLLPGNEALTLAPFHTHYPSLEDHMASVGLAVVPSSWDQPLLLGSEGPLGNPPYRLLPPSEFCPLVVPFEMEGDTTEIPGGLPPQYQKALTAREQRVQNWQRTVKEAKLNKEQRRQFQALVEQKFHEWLLESGHRQQLDSLIPAPPETKQAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAU5</accession>
    <name>W5MAU5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Zinc fingers and homeoboxes 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZHX family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAU5"/>
    <dbReference type="FunCoup" id="W5MAU5">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005504"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005512.1">
        <property type="protein sequence ID" value="ENSLOCP00000005504.1"/>
        <property type="gene ID" value="ENSLOCG00000004596.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RC6G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182893"/>
    <dbReference type="HOGENOM" id="CLU_009147_1_0_1"/>
    <dbReference type="InParanoid" id="W5MAU5"/>
    <dbReference type="OMA" id="ACEPEDD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004596">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002779">
        <property type="entry name" value="Zinc fingers and homeoboxes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000062">
        <property type="entry name" value="zinc fingers and homeoboxes protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000133">
        <property type="entry name" value="zinc fingers and homeoboxes protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000212">
        <property type="entry name" value="Zinc fingers and homeoboxes protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024578">
        <property type="entry name" value="Homez_homeobox_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041057">
        <property type="entry name" value="ZHX_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467:SF6">
        <property type="entry name" value="ZINC FINGERS AND HOMEOBOXES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467">
        <property type="entry name" value="ZINC-FINGERS AND HOMEOBOXES RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11569">
        <property type="entry name" value="Homez"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18387">
        <property type="entry name" value="zf_C2H2_ZHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="18">
        <location>
            <begin position="303"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="18">
        <location>
            <begin position="486"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="18">
        <location>
            <begin position="609"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="18">
        <location>
            <begin position="736"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="305"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="488"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="611"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="738"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="550"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="651"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="717"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="651"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="685"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="727"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="858" mass="94888" checksum="0236E807479E8C35" modified="2014-04-16" version="1">MASKRKSTIPCMIPVKSMHIQEDLEQEGGTPDSPPSIDLFPPSEEAGPDPMEPCEPTRTEVGHAQRCGGPYTCQPCDFESQDLNLFLDHMNSSHPNFRAEPSFCCLECGVSTKNYEGLALHNARAHPSAVPGGSSTGVTLQVSKKDRKTTVEQTVVAGEAGPEGAKESEISITKTPIMKMLKGKSEPKRIVVSHSVEEPDTGCSKEPEKRETPTVTAMATIVHNGPPSKVTLPSAIQIVNGSNALPVLKTAITQVVSVVQNRSLQQPAPISLSSTSSKNLPKVMIPLSSIPAYNAAMDTNSYLKSSFSKFPYPTKAELCYLTVVTKYPEEQIKIWFTAQRLKQGISWSPEEIEDARRKMFNTIIQTAPPHHSSHPHTITVLPAPLGSNGMPHIIQGSLVGQGGVIVTQPMMANGIQVSSAPMALAVTPKPQMAAKPQLQARPSTALVADKGAKVVDTAPTIANITHSTRTLPSSILDPSFYKNKKSQEQLTALKQSFLRSQFPEQEEVERLTKITGLTIREVRKWFSDRRYHYRNLKGTRSSAGGLFSGNSVLDLADSPSSVGSPTSPQHQHTPQQPQQQATPSTPSRRSSWHQTPDFTAIRYKERDPQQVRALEASFTQNPLPSPEEVDRLRAETKMTRREIDGWFSERRKKVAAEKKKEEEEEEEGGEEEKEEPSRGKGVQKRGKEEKQKEVSASEPKVNPIKINLKMLKVTESNGKPDAEASSPALTESPRTLPSVSPRSKKTPEQLHLLKQLFARTQWPSNTQYEDVATQTGLPRPEVVRWFGDSRYVCKNGQLKWLESYQRMVEEEGKEDTKVLADYLASHKKLEEEHVKGLADSSGLSEEQVRQWFVKHLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFT8</accession>
    <name>W5MFT8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="25">PARP</shortName>
            <ecNumber evidence="25">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="25"/>
    <dbReference type="EMBL" id="AHAT01025153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFT8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007247"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007256.1">
        <property type="protein sequence ID" value="ENSLOCP00000007247.1"/>
        <property type="gene ID" value="ENSLOCG00000005988.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159911"/>
    <dbReference type="HOGENOM" id="CLU_004303_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFT8"/>
    <dbReference type="OMA" id="CKLLLQX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005988">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904355">
        <property type="term" value="P:positive regulation of telomere capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070198">
        <property type="term" value="P:protein localization to chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09524">
        <property type="entry name" value="SAM_tankyrase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01438">
        <property type="entry name" value="tankyrase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000012">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000009">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000010">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000011">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000021">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000024">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase tankyrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="19 24" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 25" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 25" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0779">Telomere</keyword>
    <keyword evidence="8 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="373"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="409"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="442"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="535"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="568"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="601"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="688"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="721"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="24">
        <location>
            <begin position="754"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="27">
        <location>
            <begin position="878"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="28">
        <location>
            <begin position="960"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="26">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00033987"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50105"/>
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            <dbReference type="PROSITE" id="PS51059"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007247.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="1167" mass="126903" checksum="CFD8DC0F83AB0745" modified="2014-04-16" version="1">TGSPPMSARRCSGTSGPGGGSAPAGTEVPGGGEPTRELFEACRNGDVERVRKLVTPENVNSRDTAGRKSTPLHFAAGFGRRDVVDFLLLSGANVHARDDGGLISLHNACSFGHAEVVSLLLRHGADANSRDNWNYTPLHEAAIKGKIDVCIVLLQHGADPLIRNTDGRTALDLAEPSTKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKVVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLAYEFKGHTILQAAREADVARIKKHLSLETITFKHPQTHETALHCAAASPYPKRKQVCELLLRKGANVNEKTKDFLTPLHLASEKSHNDVIEVLVKHEAKVNALDNLGQTPLHRAAHCGHLQTCRLLLSSGCDPLIMSLQGFSPSQMGNESVQQILQEGVLIGNSDADRQLLEAAKSGDLEIVKKLCTLQNVNCRDVEGRQSTPLHFAAGYNRVAVVEFLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVTHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLCSPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGAEVNSQDKGGLIPLHNAASYGHVDVAALLIKYHACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQSPLDLVTADDVRTLLTAAMPPSALPSCYKPQVINVSAAAAGGPAPASLSSASSLENLSVAFPEMPGLLGTSGAEGPVDRKEEVLGVDVSINQFLKNLGLEHLLDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKVIKGVERLIAGQQSLNPYLSLNATNSGTILVDLAIEDKEFQSVEEEMQSTIREHRDGGHAGGVFNRYNIVKIQKVCNKRLWERYTHRRKEVSEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICQRHLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYKIVKPEMTAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFV3</accession>
    <name>W5MFV3_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Si:ch211-51h9.7</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="7"/>
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    <sequence length="151" mass="16711" checksum="76CF5B42FB3FA960" modified="2014-04-16" version="1">HGPSGAGDLLLCRACGHEVALGSDLKFVASGLALAQRNGTAVGGRRVPVQLFQNPHGSRFRVVTLRRAAVRRHWPADGHFSWFPGYSWTVATCPRCHTHLGWGFQPSDWPDRVSDREFEDSEQTFVGLIVDKLLQENFAASLLLTPKTFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFZ7</accession>
    <name>W5MFZ7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Aldehyde dehydrogenase 9 family, member A1a, tandem duplicate 2</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(trimethylamino)butanal + NAD(+) + H2O = 4-(trimethylamino)butanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:17985"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16244"/>
            <dbReference type="ChEBI" id="CHEBI:18020"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015937">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015938">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015211128.1">
        <property type="nucleotide sequence ID" value="XM_015355642.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFZ7"/>
    <dbReference type="FunCoup" id="W5MFZ7">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007306"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007314.1">
        <property type="protein sequence ID" value="ENSLOCP00000007306.1"/>
        <property type="gene ID" value="ENSLOCG00000006045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690012"/>
    <dbReference type="CTD" id="100005587"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163309"/>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_1"/>
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    <dbReference type="OMA" id="WTRMLVH"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006045">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047105">
        <property type="term" value="F:4-trimethylammoniobutyraldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019145">
        <property type="term" value="F:aminobutyraldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07090">
        <property type="entry name" value="ALDH_F9_TMBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000019">
        <property type="entry name" value="4-trimethylaminobutyraldehyde dehydrogenase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000016">
        <property type="entry name" value="4-trimethylaminobutyraldehyde dehydrogenase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009725">
        <property type="entry name" value="PRK13252.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="13">
        <location>
            <begin position="56"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="524" mass="56679" checksum="C96764D8418C9204" modified="2014-04-16" version="1">MMQQCSSTAQAGLEILRTGLLPSAVIRSASTGTLELKEPLNFWCGGRVKSSTKDAKSREPVYEPATGRVLCEMVPCGAQEVDQAVQSAQAAFLKWSKLSGMERGRIMLEAARIIRERRDEIAKVEVINNGKSITEARVDIDIAWQCIEYYAGIAGTLAGQHVQLPGGAFAYTRREPLGVCVGIGAWNYPFQIAAWKSAPALACGNAMVFKPSPMTPITAVVLAEIYAQAGVPEGLFNVVQGGAETGTLLCHHPGVAKVSFTGSVPTGKKIMEMSAKGIKPVTLELGGKSPLIIFEDCDLENAIKGALMANFLSQGEVCCNGTRVFVQSKILPKFLEEVVKRTKAIQVGDPLLDDTRMGALISRPHLDRVLGFVNQAKKEGARVLCGGEPFVPKDPKLKGGYFMSPCVLDNCRDDMTCVKEEIFGPVMSVLPFETEEEVLQRANNTTFGLASGVFTRDVTRAHRVAAELQAGTCFINNYNISPVEVPFGGYKMSGFGRENGLVTIEYYSQLKTVVVEMGDVESLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGL2</accession>
    <name>W5MGL2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminopeptidase like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024342">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639705.1">
        <property type="nucleotide sequence ID" value="XM_006639642.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGL2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007521"/>
    <dbReference type="MEROPS" id="M17.006"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007529.1">
        <property type="protein sequence ID" value="ENSLOCP00000007521.1"/>
        <property type="gene ID" value="ENSLOCG00000006225.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691441"/>
    <dbReference type="KEGG" id="loc:102691441"/>
    <dbReference type="CTD" id="79716"/>
    <dbReference type="eggNOG" id="KOG2597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063255"/>
    <dbReference type="HOGENOM" id="CLU_013734_3_1_1"/>
    <dbReference type="InParanoid" id="W5MGL2"/>
    <dbReference type="OMA" id="MVCEQSD"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006225">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable aminopeptidase NPEPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine_aapep/pepB"/>
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        <property type="entry name" value="NPEPL1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF4">
        <property type="entry name" value="AMINOPEPTIDASE NPEPL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18295">
        <property type="entry name" value="Pdase_M17_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="6">
        <location>
            <begin position="340"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="523" mass="55626" checksum="39F2099D20ED8ACD" modified="2014-04-16" version="1">MANVVLEFKAAAGDSEPQSRPVLVVGRLANLQQVTWAEVKGKLQPTVSKETWQSALSALNPNPTDSCPLYLSYASVAALPSRVSRHNSPSAAHFLTRLVRSCLPGGASRCIVVVCERSEVFASACAVARAFPLFSRRSSASRRADKRLVVVEFVTVGEDNGPLDLGTLECLSSTADGVRLAARIVDTPCNEMNTDHFLEEIKAVGNELRIVPVIIRGEELREKGFGGIYGVGKAAEYPPALAVLSHTPEGATQTIAWVGKGIVYDTGGLSIKGKTTMPGMKRDCGGAAAVLGAFRAAVKQGFKDNLHAVFCLAENSVGPNATRPDDIHTLYSGKTVEINNTDAEGRLVLSDGVAYASRDLAADIILDMATLTGAQGISTGKYHAAVMTNSEEWEAACVRAGRSSGDLAHPLVYCPELHFSEFTSAVADMKNSVADRENAQSSCAGLFIGSHLGFDWPGVWVHVDIASPVHAGERATGFGVALLLALFGQASEDPLLNLVSPLGGQDRGRPEQMERDCKRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGZ1</accession>
    <name>W5MGZ1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD repeat domain 81</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005902">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MGZ1">
        <property type="interactions" value="914"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007650"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007659.1">
        <property type="protein sequence ID" value="ENSLOCP00000007650.1"/>
        <property type="gene ID" value="ENSLOCG00000006334.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1786">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151039"/>
    <dbReference type="HOGENOM" id="CLU_001454_0_0_1"/>
    <dbReference type="InParanoid" id="W5MGZ1"/>
    <dbReference type="OMA" id="AYEQFTP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006334">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035014">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052651">
        <property type="entry name" value="WDR81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44662">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44662:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH" evidence="3">
        <location>
            <begin position="333"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1771"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="685"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1175"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1620"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1692"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1124"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1201"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1258"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1273"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1692"/>
            <end position="1701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2071" mass="223173" checksum="5578F300649A0B99" modified="2014-04-16" version="1">MDWLTAAVERDLGIDRRQLAPGPRPQELVAYVPTRWVSGLRERRLQRCPRPEGLSEPEVRTLLQRSLGKLPAGWTRVCIQGMRKSRLGYALARELGGPGEGSAEDSFLSLMQEVAGRNFRRRNLWRRAYRAYVRPYAGAAEETRVPAPEALRQALQALFGCPFVPTERCSPSLSPAKEREKEACFLSAASSKSGDPLCPNVLPAECLLESPEALYVVQPYTQYSLHDIVTYSPAKLGNSHAKVLFILYQLLQAMKAFQAAGLSCGELSLQDVAVDEQLCSRLKVSLAHYECLGAEGQQQQQQEEEEEEEEEEACASNGSLRSNRAKGAPGEERCQDCHARLKRLVTDWVHGRVSNFEYLMELNRLAGRREGDPNYHPVLPWVVDFTVPSGRFRDLRRSKFRLNKGDKQLDFTYEMTKEAFAAAAAGGGGGGGFGGGLGGVGVCPGAPAQAEQLHVPHHISDVLSDITYYVYKARRTPKAVLCSHVRSQWEPNEYPASMERMQSWTPDECIPEFYTDPTIFKSIHPDMPDLDVPPWCGSCEEFVDIHRRLLESREVSQQLHHWIDLTFGYKLSGKEAVKAKNVCLHLVDNHTHLTSFGVVQLFDQPHPPRLAPHVFSPPEPPPLGSATIFVPPWAAPASEAGAPAGPQVTDSLDGLVLEATGCEASIWTVVDKDEELEQGTEALDSLGVAPGSGSAFSSPAASASGKAAGPVEQLTPASVASQSLVFPADGTAIIPAAGGSGIRGAAAQRSSGLGRRQGEGGGAGGSAGVEDFKISLPEGFGSLQPLEELERLNNFLVRGLHAKAQDGRDSQGEEEDGGSSGAAASLTELFQRDMQALGVLIAEIFYAPKLRSLKPGASLKDRYQAVLKLCSSSLREVPLPLHHALDILLQRGRGGGPGAGGRISLFAYPPVCEGLPPPSPRQFLSPHTSPLPFPAYFPALHRFVFSYHARVGAEVPRGPQGTQERDIVFHLWHQLETLLKGSITPEGLEILLPFVLALMSDESTAVYAAWYLFEPISRALGPRNAAKYLLKPLVGVYDSPRCLRGRFYLYTDCFVLQLIVRLGLQPFLSSLLPHVLQILPGFEAGGDGGVGGGGGGTGPGSEWESRKALSRAGDLEEEEEYESGEGGPAAGSAGGKVGAGVGGGGGGVDGGLVDYSSGISLNEVFLPEGEDFQNGLYVGNGSGGAPGKQPGQSAGRDDQESLSTGKLSDKSSASEVSLGDGDSGKDRASLKSADSSQDLKQASEGEEDLRELEEEEGGPCPELALSERAGAAAGAGEGGNGEGTELGEEKGPEEDVEEEEEEEEEHDLSEDSDEKEHKILLDTLCKTVRWLAAKLGPTVTSRYLARNLLRLLTICYVGPDKHQFVESVVEDGSLDNVVNVYEKQPVVGDQMAGPVLGCLMYIAHLYGEPVLTYQYLPYVGYLVSPPSSCRLNTRKEAGLLGAVVLTKKIVVYLSDTTLMDMLMKLNQDVLLPLLDLLTSPRMGFPSGVQTRSALCLKTISLMALICLRIGREMVQQHMADTLRKFFQVFSLLQVLQTQVDTAPRKEVGDTTVLELCTPDGSEVTCELSVLEELQAVFNPEMAYASYIPFSCLIGDTAMRKVLPNYKLVCQLAVSYQEGISPDGGKPATSQRGEPLASSVGFPAGYGNGTGPSPFPAPSPSFNGDPIPESGTFGSVLVGNRIQLTRDADAGGGAGGLLGDGWGRPSSTHSQKGPAPSCSGGEHGPKDSALKQELARSGRSLSGNWLAYWQYEIGLNQQDPHFTFHQIRLQSYLGHTGTAKCLAPLTGEDFFLSGSKDKTVRLWPLYNQGDGTWDSEPRLTYTEHRKSVFYVGQLDALQEVVSCDGTVHLWDQFSGKQIRTYEVSDSKNPVTAVTTMPAPHCSVVFGSADSALRFIDPRKPGLQHEFRLAYNNISAGLIRYLTVSPNGRTIAAGFSSGFVVLIDARTGLILRGWPAHEGDILQMKAADGSLLVSSSTDHTLSVWKELEQKPLHTYRATSDPIHTFDLYGPEIVAGTVANKISVYSMLDVSASPASTTKLSSENFRGTLTSLAVLPTKRLLLLGSENGAIRLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH05</accession>
    <name>W5MH05_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox protein Hox-C6a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH05"/>
    <dbReference type="FunCoup" id="W5MH05">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007664"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007673.1">
        <property type="protein sequence ID" value="ENSLOCP00000007664.1"/>
        <property type="gene ID" value="ENSLOCG00000006348.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159254"/>
    <dbReference type="HOGENOM" id="CLU_046428_0_0_1"/>
    <dbReference type="InParanoid" id="W5MH05"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006348">
        <property type="expression patterns" value="Expressed in zone of skin and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000879">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006711">
        <property type="entry name" value="Hox9_activation_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017112">
        <property type="entry name" value="HXA9/HXB9/HXC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04617">
        <property type="entry name" value="Hox9_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="183"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="374"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="185"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="376"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="322"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="469" mass="53136" checksum="2ED41423A741DAE0" modified="2014-04-16" version="1">MSATGPISNYYVDSLINHESEEVLAATRFSAPGALPSASRPAGLVPECTDFPSCSFAPKPPIFTTSWAPVQSQSSVVYHPYTHQPHLGADSRYVRSWLEPISGAVSFPGFPANSRHYGLKTDAFPERRAGDCLAASGRNYSDYLYGSSADIRDRTPQNIPSPETEVLAPSKHKEEKAEIDPTPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKFPGGQDVLPNVALNSTTYDPVRHFSSYGAAVAQNRIYSSPFYSPQENVVFGSSRGPYEYGSNVFYQDKDGLPSCRQNSMGQSAQSALAPDYSPDQGRTGAQQQKGSVQIYPWMQRMNSHSGVGYGSDRRRGRQIYSRYQTLELEKEFHFNRYLTRRRRIEIANALCLTERQIKIWFQNRRMKWKKESNLTPAVTGGGAAGGSQEEEKEGIEEETEDEKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI02</accession>
    <name>W5MI02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637741.1">
        <property type="nucleotide sequence ID" value="XM_006637678.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI02"/>
    <dbReference type="FunCoup" id="W5MI02">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008011"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008021.1">
        <property type="protein sequence ID" value="ENSLOCP00000008011.1"/>
        <property type="gene ID" value="ENSLOCG00000006635.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687043"/>
    <dbReference type="KEGG" id="loc:102687043"/>
    <dbReference type="CTD" id="84984"/>
    <dbReference type="eggNOG" id="ENOG502RZP1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016356"/>
    <dbReference type="HOGENOM" id="CLU_113348_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI02"/>
    <dbReference type="OMA" id="AKKCGIQ"/>
    <dbReference type="OrthoDB" id="2163581at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006635">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097712">
        <property type="term" value="P:vesicle targeting, trans-Golgi to periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="160" mass="18734" checksum="EC53C45C629A815D" modified="2014-04-16" version="1">MTYTAKKCGVRFTPPAIVLIYEDAVTQKARKRIMPVRSFSKFSDCSKAAERLKNNVRHSAYLQDVSMRQMERLHVILRDRLLGRTLEQSLASLRLDPNEDLNKLGDEELSRKKARMDQLFEQNRKKKDDPDFVYDVQVDFPQDERLETCSWDEEESDGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIZ5</accession>
    <name>W5MIZ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01040445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040447">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040450">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIZ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008354"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008364.1">
        <property type="protein sequence ID" value="ENSLOCP00000008354.1"/>
        <property type="gene ID" value="ENSLOCG00000006907.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161708"/>
    <dbReference type="InParanoid" id="W5MIZ5"/>
    <dbReference type="OMA" id="SKVYRMP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006907">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000674">
        <property type="entry name" value="Mucin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF264">
        <property type="entry name" value="MUCIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="10"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="358"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="834"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1263" mass="138691" checksum="5486B46BD05901B8" modified="2014-04-16" version="1">ALFCPVGEVGVCSTWGFGHFHTFDNFMYDFSGTCNYILASDCRSLTPAFNVQMRRDALGSIQCIVIELPSSTVRVNREGITLDGRNIPLPFFSEGVLIRTFGILVRLEVKPINLIVMWNREDYLTVEVGENFQNRTCGLCGDFNGDPLKSEFEGNLIPFQFANEQQEDDPNEACLPLESSCSSGIICIEYAYLCKELLDKVALHCPVDKELYVIRCQQDLCLGPEKGQVQSSCSTLTEYARVCARIAPETVSRWRTASLCTVGHCPENQVYLETGSPCRQTCSRPRFRCPAVYNPGCFCPNGTVLDNISEKSRCVQLEECPCVNNGHKYRAGQRRESRCESCVCLNSRWECKERFCPHTCAVEGGSHVTSFDSHKYRFHGSCTYVLLKSALLPWGGMVMGHFHKCGAGERQSCLTAVSFASTQVPDPCGPAPSRAASHPAKMTSSPPPEHFVIFNQSSSFLQVDMWPWLTLQLQLQPVLSVYLRAEKKLFGLTKGLCGNFNDNSRDDFKNSMGIREGIASRFVHSWRLRSRCASPRNTDPDPCRWSEADAEYASTHCRALLNKSSVFGQCHGEVNPLSYFKRCLYEVCNYEETQDYLCGALQSYSRACAEKGMVLKNWWNETVHCGEPGASAVTCPQNQEFMQNGSLCGRTCRSLSPGATCSPSPVLLEGCSCPPGTYLDQLGSCVPPASCPCFEDNRYFPAGSAVQLDGLTCLCSSGVLDCEGSRGLLPQDDLSSLCTPPKIFHSCVNETQKACEPTCQDLRIGAICMTCESECFCPNGQYEDELLGCVAPEDCSCQQGETFYQANTVLQTECQSCTCRAGRWDCVMRSVCPGTCVQYGEGHFRTFDDRRFVFDGSCAYTLLQDTCDGADQGRFSLESKVVLCETLGPTCSRAVRLTVGDVSIELAGETYTITPPSASANFTVHDNSLYLVVTATIPYSSNKLVLIWNRNMNLIVKLMKSTQMSLCGLCGNFNGKASDDFRLRSGDLTSDAVAFANSWRTDPGCPLVQEQPSPCDTNPQRRAWAHRKCDIIRSPVFEPCHAQVYYLPFYDACVRDACGCALGGDCKCVCNAVAMYAKECLDQGVCVDWRRPDFCPVYCEFYNSHQLAGASYHYTGNGSCSWHYQPCLCPFQTQGLSSTQNLEGFLPECECSSILSRSRGGYGTQCLPLPIAVSTQTVPPITTAVPLVTAVPSLATTAQQVNETSVPPVTTAVSPVTTSRPLNTTAVSPVTTSRPLNTTAVSPVTGAWPLNTTAVSPVTASRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJP3</accession>
    <name>W5MJP3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iroquois homeobox 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020901">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJP3"/>
    <dbReference type="FunCoup" id="W5MJP3">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008602"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008612.1">
        <property type="protein sequence ID" value="ENSLOCP00000008602.1"/>
        <property type="gene ID" value="ENSLOCG00000007105.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159483"/>
    <dbReference type="HOGENOM" id="CLU_048118_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJP3"/>
    <dbReference type="OMA" id="CPFPNSA"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007105">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072498">
        <property type="term" value="P:embryonic skeletal joint development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032330">
        <property type="term" value="P:regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF17">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="117"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="327"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="52041" checksum="B6C30F76124C0E05" modified="2014-04-16" version="1">MAYPQGYLYQPSASLALYSCPAYSTSVISGPRTEELGRSSSGSAFAPYAGSTAFTSPSPGYNSHLQYSADPAAAATFTSYVGSPYDHTPGMAGSIGYHPYAAPLGSYPYGDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEENIDLEKNDDEEPHKPTEKGDSTDTESGEHKPNPGEITCDRFKDEGPGKEIDPILTDSELKDHEDRTEILPESPRPTTSTPSAVPRGTEIIAHDKPAEMVHASSTGNSVHSNSVVTSVIHSPPVAPKPKLWSLAEIATSSDRSKGSTDVPQPAGPAQSSVIAGTPTSPSRSPSQCTFPNSTVLSRPIYYASPFYAGYTNYGTFGHLHSNHGSTTGSTAHFNGLNQTVLNRAEALVKESKVRVHTQVDLCKDSPYELKKGLLDCVKLWKTVTNVQKQVHPTHFSCYQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKE7</accession>
    <name>W5MKE7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Prostaglandin E synthase 3</fullName>
            <ecNumber evidence="4">5.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cytosolic prostaglandin E2 synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>prostaglandin H2 = prostaglandin E2</text>
            <dbReference type="Rhea" id="RHEA:12893"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="5.3.99.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.3" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01032420">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKE7"/>
    <dbReference type="FunCoup" id="W5MKE7">
        <property type="interactions" value="2010"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008856"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008867.1">
        <property type="protein sequence ID" value="ENSLOCP00000008856.1"/>
        <property type="gene ID" value="ENSLOCG00000007299.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154256"/>
    <dbReference type="InParanoid" id="W5MKE7"/>
    <dbReference type="OMA" id="IEHKVTD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007299">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050220">
        <property type="term" value="F:prostaglandin-E synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905323">
        <property type="term" value="P:telomerase holoenzyme complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00237">
        <property type="entry name" value="p23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000003">
        <property type="entry name" value="prostaglandin E synthase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF3">
        <property type="entry name" value="PROSTAGLANDIN E SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="17">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="146" mass="16965" checksum="22DDA3B6AAD079BD" modified="2014-04-16" version="1">QPASAKWYDRRDYVFIEFCVEDSKDVQVNFEKSKLSFSCLGGTDSLKHQNEIELFEGIDPNESKNKRTDRSVLCCLRKTESGKSWPRLTKDKAKLNWLSVDFNNWKDWEDDSDEELSSFDRFSEMMNNMGGDDDLPEVDGAEDVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MML3</accession>
    <name>W5MML3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Selenoprotein J</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MML3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009622"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009633.1">
        <property type="protein sequence ID" value="ENSLOCP00000009622.1"/>
        <property type="gene ID" value="ENSLOCG00000007922.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUJI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000023530"/>
    <dbReference type="HOGENOM" id="CLU_046767_1_0_1"/>
    <dbReference type="InParanoid" id="W5MML3"/>
    <dbReference type="OMA" id="QANNFVK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007922">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF17">
        <property type="entry name" value="SELENOPROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="35754" checksum="50385A221088AA78" modified="2014-04-16" version="1">MALTLEDRILGAIIGAAVADAAAQPLHWIYDLKMLHTILSNEPCPEFRPQSANPFYRRETGQQSCYGDQAFVLLESLTECGGLNVDDLKQRTYKFFGPGSEYDTPGPRPQLPIEGPWRHASLKSFLKNFEAGKTDTGCEVDNQIDGIAKLAPIVALYAGKPELLEKVKVAICVTQNSDTCVAVTLAAARFLEYYILHGPDQRALDVVLDQLKNQNSMNPQNLDGPVYGWWINFVMFDTCFRDHSERLPGAFQAALHGVLTETVFEQAIRNTMSCGGCTSSRSSFIGACLGAQVGLSGIPDSWKIRTLRYPILLELAKKVAQLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMY3</accession>
    <name>W5MMY3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMY3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009753.1">
        <property type="protein sequence ID" value="ENSLOCP00000009742.1"/>
        <property type="gene ID" value="ENSLOCG00000008007.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074829"/>
    <dbReference type="OMA" id="WIFKNIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008007">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="191" mass="21119" checksum="9A586FCC4EE144A3" modified="2014-04-16" version="1">MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPKRLFEGNVLTYSGSGRGVGAVGLNLCLGLAISVLKRRRCGIAPGLAGLSAPPHAANLPPSPLPRRVRKQVVNIPSFVVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGGADEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN34</accession>
    <name>W5MN34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING-box protein 2</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
            <ecNumber evidence="17">2.3.2.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">RING finger protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Sensitive to apoptosis gene protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Catalytic component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) composed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B; additional evidence is however required to confirm this result in vivo. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2. May also interact with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="EC" id="2.3.2.32" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01015290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637775.1">
        <property type="nucleotide sequence ID" value="XM_006637712.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN34"/>
    <dbReference type="FunCoup" id="W5MN34">
        <property type="interactions" value="1018"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009793"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009804.1">
        <property type="protein sequence ID" value="ENSLOCP00000009793.1"/>
        <property type="gene ID" value="ENSLOCG00000008065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693771"/>
    <dbReference type="KEGG" id="loc:102693771"/>
    <dbReference type="CTD" id="9616"/>
    <dbReference type="eggNOG" id="KOG2930">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155481"/>
    <dbReference type="HOGENOM" id="CLU_115512_2_2_1"/>
    <dbReference type="InParanoid" id="W5MN34"/>
    <dbReference type="OMA" id="RQNNRCP"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008065">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031466">
        <property type="term" value="C:Cul5-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061663">
        <property type="term" value="F:NEDD8 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="RING-H2_RBX2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING-box protein 2 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044896"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069656"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078874"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="113" mass="12760" checksum="721E0B6D764D742B" modified="2014-04-16" version="1">MVEMDDGDEPVLIPSHSGGSGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPV9</accession>
    <name>W5MPV9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020067">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214447.1">
        <property type="nucleotide sequence ID" value="XM_015358961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010418"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010432.1">
        <property type="protein sequence ID" value="ENSLOCP00000010418.1"/>
        <property type="gene ID" value="ENSLOCG00000008549.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696586"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156972"/>
    <dbReference type="HOGENOM" id="CLU_002289_5_2_1"/>
    <dbReference type="InParanoid" id="W5MPV9"/>
    <dbReference type="OMA" id="IRDEAWV"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008549">
        <property type="expression patterns" value="Expressed in camera-type eye and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF32">
        <property type="entry name" value="SODIUM-DRIVEN CHLORIDE BICARBONATE EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="602"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="704"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="830"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="890"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="962"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="985"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="17">
        <location>
            <begin position="125"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="16">
        <location>
            <begin position="460"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="256"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="414"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1095" mass="123399" checksum="CBD6EE4997563A6A" modified="2014-04-16" version="1">MDIKDQGAQMEPLLPTVLTTSGNKSVRNDEEAVVDRGGTRSILKTHFEKEELEGHRTLYIGVHVPLGGRKSHRRHRHRGHKHRKRDRERDSGVEDGRESPTYDTPSQRVQFILGTEDDDEEHIPHDLFTELDEICVRDGEEAEWRETARWLKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGIVLLDMRANMLEEIADMILDQQESLGQLSDDVRKKVRHALLKQHHHQNQKKLSNRIPIVRSFADIGKKQSEPHSMDKNGQMFSPQSMPTNTEGKGDVSRENSTVDFSKVDLHFMKKIPPGAEASNILVGELEFLDRPVVAFVRLSPAVLLTGLAEVPIPTRFLFILLGPLGKLQQYHEIGRSIATLMTDEIFHDVAYKAKDRNDLVAGIDEFLDQVTVLPPGEWDPSIRIEPPKNVPSQEKRKMPAVPNGTAALGEPEEHGGHGGPELQRTGRLFGGLIMDIKRKAPHYLSDYTDAVSLQCLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFAGQPLTILGSTGPVLVFEKILYKFCREYGLSYLSLRACIGLWTAFLCIVLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLIHLGETYPINMNNNLEKLTQYSCICVEPPDPSNATLTFWDEHNITASEIYWEGLEVHDCIEKHGEFVGRACGPHGPYVPDVLFWSVVLFFSTVTMSSFLKEFKTSRYFPTKVRAIISDFAVFLTILTMVLIDYGLGIPSPKLQVPNEFKPTRDDRGWLVNPLGPNPWWTTIVTVFPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDLFMVGVMLGVCSIMGLPWFVAATVLSISHVNSLKLESECSAPGEQPKFLGIREQRFTGLMIFLLMGCSVFMTSVLKFIPMPVLYGVFLYMGGSSLKGIQLFDRFKLFGMPPKHQPDFIYLRHVPLRKVHLFTVIQLSCLVLLWVIKTSRAAIVFPMMVLALVFIRKVLDFLFTKRELSWLDDLMPESKKKKLEDAQEEEEQSMLGEEEGIVHLPLEGNYKDDPSTVNISDEMAKTSVWKTLTMNTENSKKDSCIPSKSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQV1</accession>
    <name>W5MQV1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADPH oxidase organizer 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01013724">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013725">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQV1"/>
    <dbReference type="FunCoup" id="W5MQV1">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010760"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010775.1">
        <property type="protein sequence ID" value="ENSLOCP00000010760.1"/>
        <property type="gene ID" value="ENSLOCG00000008840.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW5I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158812"/>
    <dbReference type="HOGENOM" id="CLU_030529_2_1_1"/>
    <dbReference type="InParanoid" id="W5MQV1"/>
    <dbReference type="OMA" id="EACCISP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008840">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12024">
        <property type="entry name" value="SH3_NoxO1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000219">
        <property type="entry name" value="NADPH oxidase organizer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000233">
        <property type="entry name" value="NADPH oxidase organizer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000040">
        <property type="entry name" value="NADPH oxidase organizer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035758">
        <property type="entry name" value="NoxO1_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF10">
        <property type="entry name" value="NADPH OXIDASE ORGANIZER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="169"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="242"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="489" mass="54364" checksum="3C64A828E265DD97" modified="2014-04-16" version="1">RAAMSVPRYPVDVRLIGVMHKNGSNMFMTSVFWSDQSDIVVYRSFGEFRKLHKQLKKKFPPENMIRKSDSVLPKFKARHLKTLFQAKGPGRSVIRLKCVEKYCGELLRCDPEISQSAEVIRFFLPQSQDLQPEFPKNSFVIMPPEDPEDSPRAGVDGKRMSEGNVTHPFVTETYQCVAPYETKDTKNQPFRVAVDEVVDVLIKDPAGWWLVENEDKQLAWFPAPYLRRNEDEGEEELLDAHEEGGLYCAVRTFEAKNADELSVNIGSVVQVIQTSDDGWWLSSYKGRAGYVPSMYLQPYRSPPLGFRSLAPAHRQGLSRSQELLRPAQSAAAVPPMGTADRKKWCSLDVLSDPRPGPVPTITVEAEREEPGSRKDSLSGDSELGCSSESFSSSSEFLGASLSDAPGPLGRSRTPQALVADRQSPDSGAPEQSLSSSSSEPNLYKPPSAPRVPPRPKAQEILTRCTTITCKAALASKCRLSPETEEIQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQW7</accession>
    <name>W5MQW7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-uridine aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TDD superfamily. DTWD2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01002811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQW7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010776"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010791.1">
        <property type="protein sequence ID" value="ENSLOCP00000010776.1"/>
        <property type="gene ID" value="ENSLOCG00000008859.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006867"/>
    <dbReference type="HOGENOM" id="CLU_066458_3_0_1"/>
    <dbReference type="InParanoid" id="W5MQW7"/>
    <dbReference type="OMA" id="GTWRKAF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008859">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016432">
        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039262">
        <property type="entry name" value="DTWD2/TAPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392:SF0">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="7">
        <location>
            <begin position="56"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="293" mass="32938" checksum="40D8669F0BC6E7CE" modified="2014-04-16" version="1">METESLGVSALSLADECCSTEEDLVKLTTTSEEVEKEKNSGGFEDLLELPVESPERRPTCLRCSRPQKVCLCPFLPVHPLEVSTCLYIVQHPAEESRVLRTVPLLAACLPPGKCKVLIGRRFNEERYPELASVCQTPGTLILYPGSDAENLEELDLNSSSAAYNIIIIDGTWSQAKDMFYRNSLFRLPKQVQLKTTLSSQYVIRTQPTNTCLSTLECAAIALSIMEKNDSIQEVILRPLKALCSFQLEHGAQVHHSKEHLLKNGLYDKPMPKNKRKLKRMERLLNILYCICPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSV9</accession>
    <name>W5MSV9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in protection from oxidative damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004787">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSV9"/>
    <dbReference type="FunCoup" id="W5MSV9">
        <property type="interactions" value="1541"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000011485.1">
        <property type="protein sequence ID" value="ENSLOCP00000011468.1"/>
        <property type="gene ID" value="ENSLOCG00000009399.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155187"/>
    <dbReference type="InParanoid" id="W5MSV9"/>
    <dbReference type="OMA" id="THIEGVC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009399">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061550">
        <property type="term" value="P:cranial ganglion development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902882">
        <property type="term" value="P:regulation of response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.350.10:FF:000002">
        <property type="entry name" value="Oxidation resistance protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF69">
        <property type="entry name" value="OXIDATION RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="7">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="731"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="892" mass="99105" checksum="EE6ABC78CFD52AFF" modified="2014-04-16" version="1">MATVLSHFSFFNCCRLKKKSQSVDITAQGYSGPLVADTPASQITRPAPPVAKTTTSEEEDNNASNSQKRNPRRSELKRYYTIDTGQKKTPEKKDGRRMSFQKPKGTIEYSVESRDTLNSIALKFDTTPNELVQLNKLFSRAVVPGQVLYVPDPEYISSVESSPSISPISPLSPTSSEAEVEKVTDSDGVPRKEATSSPAHACTRPARVVSSTSEEEEALTEKFLKINCKYITDGKGAVSGVLLVTPNNIMFDPHKTDPLVQEHGCEEYGIMCPLEEVMSAAMHKEIVDSKIKESLPPDLEHLASGRDPCHLKRIPRSNTDEMELRLRDTGNDSTSTAPRSTEESLSEDVFTESELSPIREELVSSDELRQDKSSGASSESVQTINQTGADCCLVSETGEPPKPSAEDTPTQDRGLVRHRAEGEKSEPAGNPEENPPRSQTPKQSPMGSKQHSTDKEPSTPACKDQHSSCKGDAAKSRAGSDSQGPGAAAGDPSGREGPEKSSLSREQSCDSETEVQELRKLWKTHTMQQAKEQRQHVQQPHKEVKPKPGKVIEGLLPLEGSVVCKEKRRHRSHKFLCLRVGKPMRKTFVSHASASMQQYAQRDRKHEYWFAVPQERSDHLYVFFIQWSPDMYGEGAGGLSREPGFMVVKKNEEPESAEEPLATDVTAKEWEVVSVAEYHRRIDALNTEDLRSLCRRLKITTKEEVNSTQGTSIKTDLEPDTFRPNLSEPSDLLQPEQIEKLAKHLPPRTIGYPWTLAFSTAKHGMSIKTLYRTMAGQDTPVLMVIKDSDGQIFGALASDPFKVSDCFYGTGETFLFTFCPEFEVFKWTGDNMFFIKGDMDSLAFGGGGGEFGLWLDGDLYHGRSHSCKTFGNHTLSKKEDFYVQDIEIWAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSX6</accession>
    <name>W5MSX6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell cycle control protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSX6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011485"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011502.1">
        <property type="protein sequence ID" value="ENSLOCP00000011485.1"/>
        <property type="gene ID" value="ENSLOCG00000009411.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004660"/>
    <dbReference type="InParanoid" id="W5MSX6"/>
    <dbReference type="OMA" id="NDIFWLH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009411">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015247">
        <property type="term" value="F:aminophospholipid flippase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF68">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="372" mass="41801" checksum="2CFD163A4E269A66" modified="2014-04-16" version="1">TRLSKKKRKEEGHNCSSGVGNMPKENDNIARRPDSTAFKQQKLPAWSPSLTAETVLPFFFLIGLLCVLTGAWLLVTVAATHEIKIDYTNTGNCSDCYELRENRLNSTKDCQCTVTFSLPEQIVGDVYVYYGLINFHQNLRRYMDSRDDAQLVGRTFNLKNPSSTCAPFDKYKHGTPIAPCGAIANSMFNDSFQLVFHPNEGMAMSVPLLRTGIAWYTDKNVKFRNPGPYNESLSAVFAGTSQPFYWQKPVFELDPTDTKNDGFVNDDFIVWMREAAFPNFKKLYGRLSRFAAFADGLPGGNYSIQISYNFPVKYFQGKKLVILSTVSWFGGQNRFLPIAYTVTGGVILIVAMILTGFHLKFGKRGLQANLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT98</accession>
    <name>W5MT98_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dual-specificity kinase</fullName>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01015496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MT98"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011607"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011624.1">
        <property type="protein sequence ID" value="ENSLOCP00000011607.1"/>
        <property type="gene ID" value="ENSLOCG00000009499.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157807"/>
    <dbReference type="HOGENOM" id="CLU_018577_0_0_1"/>
    <dbReference type="InParanoid" id="W5MT98"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009499">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14155">
        <property type="entry name" value="PKc_TESK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000202">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000134">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF3">
        <property type="entry name" value="DUAL SPECIFICITY TESTIS-SPECIFIC PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="44"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="305"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="396"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="689"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="715" mass="77627" checksum="015C66E21B3EFEE7" modified="2014-04-16" version="1">MDRAEPDQESSDPSVIGAAGPNRLRPSSYRALRSAVSSLARIDDFYCEKIGSGFFSDVFKVRHRITGQVMVLKMNKLASNRANMLREVQLMNRLCHPNILRFLGVCVHEGQLHALTEYINGGNLEQLLDSDVYLSWAVRMGLSLDIARGLQYLHSKGIFHRDLTSKNCLVRCENGLFTAVVGDFGLAEKIPDYSEGVEKQPLAVVGSPYWMAPEVLRGELYNEKADVFAYGIILCEIIARIQADPDFLPRAEDFGLDVESFQHMVGDCPPCFLQLAVNCCNMSADQRPSFSDIVVELERREREKQILKEENGSPVRPLVAPGSSPARRRSVGLPHDRGLSRSQSDMLPPNSPPLLSGTPARVNPFSQRHDLNGGKIKLFDTPSKSVISLTFALPPPPDPFTGSSPTPPDLWQDSRGTPRRCRSLPCTPELPRPLRPVVAPTEKQGTPGQQSRGVMIVSTFQDREGKGQEIPEDVGQMFGPGHWDEPIRRQPAVRPQDIGGRGQIAQDLGSGGETTEDSGTPVDLELVSLEMLGEGELETLCLGEPMDCSSSPDTLEGSVFLEGTPAGQTWLTPPSPSSSILANGWESPVFNGPPSLPPLPPLDNNNTTVVVSRPLGWGGNGYHGHTGGPAYPLHGSSTATLEQDEVISCPGCCLAGFSFPSACLRPSRRNPPPYKNLNGEAARGLLCRNAKTLGPSQHTPGHSAEPSLPLPEAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MTJ7</accession>
    <name>W5MTJ7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HEAT repeat containing 5A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEATR5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003854">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011706"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011724.1">
        <property type="protein sequence ID" value="ENSLOCP00000011706.1"/>
        <property type="gene ID" value="ENSLOCG00000009580.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006205"/>
    <dbReference type="HOGENOM" id="CLU_000652_0_0_1"/>
    <dbReference type="InParanoid" id="W5MTJ7"/>
    <dbReference type="OMA" id="ETNSICI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009580">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000262">
        <property type="entry name" value="HEAT repeat-containing protein 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25468">
        <property type="entry name" value="HEAT_HEATR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="858"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1454"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1473"/>
            <end position="1484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1484" mass="159697" checksum="900B6DB27ACD8D58" modified="2014-04-16" version="1">MTLGPAVVECHVPRILLLWRCAFPLSVKDLDTERSRGDSFTWQVTLEGRAGALCAMKNFVVHCGDLLNDDVIGRLLPPLTCAVALLTQLPSLLKVYGNPMKNISMVFRQRLYEILAFLPPKTYEESFRAVLRQLVSDLTGPESPVCAGPVLLPPLCHADDVFLLGPALQDTDHHFIEEQLHQGGGAGGGSLEHDVFVISEKGWRVPAPLPPALAVVVAAARLFGVVFPQLAAAQRAQILEQFLESLKQLKGARQQAAQIHTVSAFCCALKRLGTCRGGLGSEELRKPALALLMGALESANAQLRCAAAEGLSRLAQAANDAAFTAGVTQISFDKLKSARDVISRTGHSLLLGSLYRYLGGISSSQHLGACVGILYTLSQDSTSPEVQAWALHSLSLVIDLAGPLYHAHIEPTFTLVLRLLLSVPPAHVEVHQSLGRCLNVLITSLGPELQGSSSAVSALRTSCLVGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLTSLVPSLCEILLDNSVLVNLCSSYLSLRRAVLACLRQLAQREAAEVSEHAVALVRDLPRRDSTALDIMIKETGLEGALFSLLDREHDQRLCQDIKETLGHMLSSITAGKLAHWLKLCKDVLSASADFATAAPVETTQEEEEGDRAEDTSVFTCRPEPSCPFTNPRWSTRVFAAECVCRVIAQCQSGDEAHCNMALAQERRLQDPAADFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLIIIRKFAGVPEPEFPGHVILEQYQANVGAALRPAFSPDTPPDVTAKACQVCSAWIASGVVSDLGDLRRVQQLLASSLVKVQAGKEVHSQLYNESTSTMETLAVLKAWAEVYIVAVESPKQSDSPLKPPRAAGSSDDPGSSSVDGLLRLVQADLATLSRLWLAALQDYALLTLPQEYASQLPAGGGAFYTAETVEHARPHYCSSWAPILHATALWLSSTGFIVVDEGPANLSRPVTPTSMGQSTSLASVKSPEDVNTDRFHLILGISVEFLCSPRSDTQMESISSCLHALQSLLDVPWPRSKIGNDQALSVELLSVLHRLVLTRESPAVQLAVLQLARQVVCAAQEHVKEKRHSAEVDDGAAEKETLPEFGEGRDTGGLVPGKSLVFATLELCLCILVRQLPQLNPKLAGSPVGQGGQPQPLTENDCKLVSAALGILSDLPSICSPEGSVSILPTVLFLITGVLREAVLRGQGSPLALVVPVALQALRAVVSSPMARTEKSHAAWTGLLRCALATLLDLWDAGESHPEVDEVSLLTALTIFLLSASPEVTTAPCLQTRCIAKFRRSLETQDPVVLTKSCQLLQTVFQSPSPVVAIPFIQALGPAIVGRLREVEKRMPQSVAELQAAQEGVRALEALVSVADEECRSQLVAVLLPILISFLLDENALASAQLPARNLHEFALQDLMRLGPQYSAVFRSLMASSPPMKARLEAAVKGNQESIKAKTAAREPRPPGKNSPSIQLKTNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY84</accession>
    <name>W5MY84_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01036085">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY84"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013343"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013371.1">
        <property type="protein sequence ID" value="ENSLOCP00000013343.1"/>
        <property type="gene ID" value="ENSLOCG00000010883.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SHRM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="HOGENOM" id="CLU_055459_0_0_1"/>
    <dbReference type="InParanoid" id="W5MY84"/>
    <dbReference type="OMA" id="YYCAVTF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010883">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868992" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="142"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="359" mass="40136" checksum="C97ABFCBFBFB616A" modified="2014-04-16" version="1">MVMIRFSVTLALFCDVCMANIDNIVQPSILKMVQPGDSVTLECFLPFDKISYMLWFKQTIGQGPRCILRSYYLSNDTTFFDEFKKSHFMVEKNTGKGFFHLIISRTKKSDTATYYCATAYTTQVMFGNGTLVIVKESGLISRSVVQQPVSVPVQQGNNVTLQCTIHTETCAGEHSVYWFRHGSGESLPGIIYTQGNRSDQCERISVAKHPTQTCVYNLPKRNLGPSDAGTYYCAVAICGEILFGNGTELEITGKGNIDQILSHPFFLGLLVSNAVCVMVIVVLNYAILKKKSNLCTGHISQQSLHEHTTVDSSNKQNEDTDVLNYAALSFADSKTKTGKKRRKTDRQSVYSEVRYEQHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYQ6</accession>
    <name>W5MYQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Distal-less homeobox 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYQ6"/>
    <dbReference type="FunCoup" id="W5MYQ6">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013515"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013544.1">
        <property type="protein sequence ID" value="ENSLOCP00000013515.1"/>
        <property type="gene ID" value="ENSLOCG00000011012.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696527"/>
    <dbReference type="KEGG" id="loc:102696527"/>
    <dbReference type="CTD" id="30569"/>
    <dbReference type="eggNOG" id="KOG0850">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159188"/>
    <dbReference type="HOGENOM" id="CLU_074733_0_0_1"/>
    <dbReference type="InParanoid" id="W5MYQ6"/>
    <dbReference type="OMA" id="GVSHGYC"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011012">
        <property type="expression patterns" value="Expressed in zone of skin and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035141">
        <property type="term" value="P:medial fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001841">
        <property type="term" value="P:neural tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021628">
        <property type="term" value="P:olfactory nerve formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071699">
        <property type="term" value="P:olfactory placode morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009612">
        <property type="term" value="P:response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000048">
        <property type="entry name" value="Distal-less homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022135">
        <property type="entry name" value="Distal-less_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF31">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12413">
        <property type="entry name" value="DLL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="285" mass="31449" checksum="EC0F4289F90F9850" modified="2014-04-16" version="1">MTGVFDRRVPSIRSSDFQSPFQHHAMHHPSQESPTLPESSATDSGYYSPAGGVHHGYCSPSSTSYGKALNAYQYQYHGVNGSAGNYPAKSYPDYSYTSAAYHQYAGTYSRVQAQSSPQEKEVPEPEVRMVNGKPKKVRKPRTIYSSFQLAALQRRFQNTQYLALPERAELAASLGLTQTQVKIWFQNKRSKLKKIMKNGELPPEHSPSSSDPMACNSPQSPAVWDAQGATRPHNPHSHQQNTNTATSNFLENPSSWYSASGAINSHLQPSNSIQHSLVLGAGTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYZ0</accession>
    <name>W5MYZ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">CLOCK-interacting pacemaker a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYZ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013599"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013628.1">
        <property type="protein sequence ID" value="ENSLOCP00000013599.1"/>
        <property type="gene ID" value="ENSLOCG00000011077.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RJ2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048522"/>
    <dbReference type="InParanoid" id="W5MYZ0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011077">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042754">
        <property type="term" value="P:negative regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000055">
        <property type="entry name" value="Placenta growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031602">
        <property type="entry name" value="CIPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648">
        <property type="entry name" value="CLOCK-INTERACTING PACEMAKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648:SF6">
        <property type="entry name" value="CLOCK-INTERACTING PACEMAKER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15800">
        <property type="entry name" value="CiPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-0497">Mitogen</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="14">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="207"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="434" mass="47736" checksum="85A6F70D4987443E" modified="2014-04-16" version="1">MLFGEVWGRSFCRTIEKLVEVVQEYPSEVEHIFSPACVPLWRCAGCCGDENLECYPSQTYNVTMQLLKIKPSESREYVEMTFVEHQTCEYASSEYLSAVEQTDAEDGQKTSAQSIKSKSQLPQLAVMGGGYPGLSPMIIMNNVLLKQFQASNCPPAVKPWGFQPAIEVIPQPQVVFLQPVISNGITNTQKGPQDKRRRSQKYLPILKSYPKIAPHPGESSSERSASGSSERSGSASARVDRASALRHRRHSREKQLSRLSSTASPTPSGLPLPLTPEPSTRKHHSSLSKTESASSGGQPTPPASSQEAPPPARSPGLAPVPPAAASPSRTDPDPDPEEKGSVLEKRNKRKRFCNTYNILHKSGLLGITLRTKELIRQNRKTQSELDRLKAHADLFVEAIRSGDPQVWTKLQLAMLECEPSMAEDGGEKEIQTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZK4</accession>
    <name>W5MZK4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP:AMP phosphotransferase AK3, mitochondrial</fullName>
            <ecNumber evidence="8">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Adenylate kinase 3</fullName>
            <shortName evidence="8">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AK3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + AMP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:29863"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01017149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627234.1">
        <property type="nucleotide sequence ID" value="XM_006627171.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZK4"/>
    <dbReference type="FunCoup" id="W5MZK4">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013813"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013842.1">
        <property type="protein sequence ID" value="ENSLOCP00000013813.1"/>
        <property type="gene ID" value="ENSLOCG00000011239.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692594"/>
    <dbReference type="KEGG" id="loc:102692594"/>
    <dbReference type="CTD" id="50808"/>
    <dbReference type="eggNOG" id="KOG3078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155120"/>
    <dbReference type="HOGENOM" id="CLU_032354_1_1_1"/>
    <dbReference type="InParanoid" id="W5MZK4"/>
    <dbReference type="OMA" id="TIAHFST"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011239">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061515">
        <property type="term" value="P:myeloid cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009142">
        <property type="term" value="P:nucleoside triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="10">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="8">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="8">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="228" mass="25348" checksum="6A6D712DEAAE1661" modified="2014-04-16" version="1">MVLRVLFRAVIMGPPGSGKGTISDRISKSFGLKHLSSGDVLRANIKAKTEIGLLMKSCIDQGQLVPDDVISRLILQELKTMEQHNWLLDGFPRTVTQAESLDSAYHIDTVINLDVPFETIKERLTSRWIHPASGRVYNTNFNPPKIPGLDDVTGEPLTQRNDDTPETVTKRLKAYETQTQPVLDYYRNKGILETFSGTETNKIWPHVHAFLSKKVSDGGGMASGKACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0I8</accession>
    <name>W5N0I8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Shisa family member 8b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0I8"/>
    <dbReference type="FunCoup" id="W5N0I8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014147"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014176.1">
        <property type="protein sequence ID" value="ENSLOCP00000014147.1"/>
        <property type="gene ID" value="ENSLOCG00000011515.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R5Y2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163663"/>
    <dbReference type="HOGENOM" id="CLU_045403_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0I8"/>
    <dbReference type="OMA" id="HNANMEG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011515">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774:SF14">
        <property type="entry name" value="PROTEIN SHISA-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774">
        <property type="entry name" value="PROTEIN SHISA-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="7">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="339" mass="37481" checksum="1627D7BAB2946866" modified="2014-04-16" version="1">TQASPLSPEVTLATPEEAEMTVPPSGSRCRGYYDVMGQWDPPFNCNAGIYLFCCGTCFYRFCCQFRQHHLDQTSCSNYDTPNWANTGRPLVPVTEIHEEPDRDRTNMIVYIICGVVAVMVLVGIFTKLGLEKSQGPQTDMTNSRIYDLLSFSSSGCSSLESARAASKSDLDRRASAHNANMEGNLIERGQEQHSFIGAQLGHADRTHTLHTCGIQTVHTLHPWTDTGATDYYKSYPMMDLPPQAKPTFQPIALHPKEKPLLPPPDLHGPLAVTLSSTPLAKTKISKTNTHPLTPSSAFRAWDPSKSHAHTRAPHAGQSANRRQGYSSKRQFSIETLPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0T0</accession>
    <name>W5N0T0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial assembly of ribosomal large subunit protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for normal mitochondrial ribosome function and mitochondrial translation. May play a role in ribosome biogenesis by preventing premature association of the 28S and 39S ribosomal subunits. Interacts with mitochondrial ribosomal protein uL14m (MRPL14), probably blocking formation of intersubunit bridge B8, preventing association of the 28S and 39S ribosomal subunits. Addition to isolated mitochondrial ribosomal subunits partially inhibits translation, probably by interfering with the association of the 28S and 39S ribosomal subunits and the formation of functional ribosomes. May also participate in the assembly and/or regulation of the stability of the large subunit of the mitochondrial ribosome. May function as a ribosomal silencing factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004552">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0T0"/>
    <dbReference type="FunCoup" id="W5N0T0">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014239"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014268.1">
        <property type="protein sequence ID" value="ENSLOCP00000014239.1"/>
        <property type="gene ID" value="ENSLOCG00000011592.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697510"/>
    <dbReference type="KEGG" id="loc:102697510"/>
    <dbReference type="CTD" id="115416"/>
    <dbReference type="eggNOG" id="KOG3212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015035"/>
    <dbReference type="HOGENOM" id="CLU_1199469_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0T0"/>
    <dbReference type="OrthoDB" id="21330at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011592">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial assembly of ribosomal large subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="IOJAP SUPERFAMILY ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043:SF0">
        <property type="entry name" value="MITOCHONDRIAL ASSEMBLY OF RIBOSOMAL LARGE SUBUNIT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="231" mass="26087" checksum="EC4B2002D86243D6" modified="2014-04-16" version="1">MNRVALSTWTKRLLPILSQTSIHTQAGRGLQTGLNAVAAVEQVWSGFYRGAEGGFPLKTCADLPRRLSHTGGTELSHVEDRAGFEVKKRTGLCPDFSIDFLVALLRQENAGDICVIRVPEEMKYTDYFVVVSGSSTRHLRAMALYAIKVYKHVKRNDDPHIQIEGKDADDWMCIDFGHIVVHFMLPETRELYELEKLWTLRSYDEQLGAIPPETLPEDFTYGAAFSNEPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1S8</accession>
    <name>W5N1S8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pantothenate kinase 3</fullName>
            <ecNumber evidence="5">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Pantothenic acid kinase 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01005445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631987.1">
        <property type="nucleotide sequence ID" value="XM_006631924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1S8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014587"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014616.1">
        <property type="protein sequence ID" value="ENSLOCP00000014587.1"/>
        <property type="gene ID" value="ENSLOCG00000011861.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686819"/>
    <dbReference type="KEGG" id="loc:102686819"/>
    <dbReference type="eggNOG" id="KOG2201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156396"/>
    <dbReference type="HOGENOM" id="CLU_011154_0_1_1"/>
    <dbReference type="InParanoid" id="W5N1S8"/>
    <dbReference type="OMA" id="FKNPDIC"/>
    <dbReference type="OrthoDB" id="275583at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011861">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000025">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000001">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF21">
        <property type="entry name" value="PANTOTHENATE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="41321" checksum="C1756B6811D8EE36" modified="2014-04-16" version="1">MKIKDAKKPSFPWFGMDIGGTLVKLVYFEPIDITAEEEQEEVECLKSIRKYLTSNVAYGSTGIRDTHLEIKDLTLLGRRGNLHFIRFPTHDMPTFIQMGHDKNFSTLHTVLCATGGGAYKFEEEFRTIGNLQLHKLDELDCLVKGLLYIDSPSFNGQAECYYYENALDPDRCRKVPYNLDDPYPLLVVNIGSGVSILAVYSKDNYKRVSGTSLGGGTFLGLCSLLTGCDSFDEALELASRGDSTNADKLVRDIYGGDYERFDLPGWAVASSFGNMIHKEKREAVSKEDLARATLVTITNNIGWIAKMCAVNEKINRVVFVGNFLRVNTLSMKLLAYALDYWSQGQQKALFLEHEGYFGAVGALLGLLNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N245</accession>
    <name>W5N245_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Probable ATP-dependent RNA helicase DDX41</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAD box protein 41</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Identified in the spliceosome C complex. Interacts with ERCC6. Interacts with FAM50A. Interacts with STING1. Interacts with CGAS. Interacts with several spliceosomes components such as PRP19 or CDC5L.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01005449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N245"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014704"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014733.1">
        <property type="protein sequence ID" value="ENSLOCP00000014704.1"/>
        <property type="gene ID" value="ENSLOCG00000011956.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156333"/>
    <dbReference type="HOGENOM" id="CLU_003041_16_5_1"/>
    <dbReference type="InParanoid" id="W5N245"/>
    <dbReference type="OMA" id="FKTIWTL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011956">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17951">
        <property type="entry name" value="DEADc_DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000449">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000657">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044113">
        <property type="entry name" value="DEADc_DDX41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="206"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="401"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="18">
        <location>
            <begin position="576"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="49"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="616" mass="69500" checksum="FEE70FFF7FF203F4" modified="2014-04-16" version="1">QKMETESRTRKRVYQEEEEASDFSDEEKYVPFVPVKQRKQQMLQKLLRLRGKGLTEEEQKDSGNEQRDDEEAIGPRSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKTSWKGPRYILSMPPARHDRVRKKYHILVEGEGIPPPIKSFREMKFPAAILKGLKKKGIVHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPIIMFCLEQEKRLPFFKREGPYGLIICPSRELARQTHSIIEYYCKLLEEEGAPQLRCALCIGGMSVKEQMEVVKHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEEDIRTIFSYFKGQRQTLLFSATMPKKIQNFAKSALVKPITINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKTSPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFKEGKKDVLVATDVASKGLDFPAIQHVVNYDMPEEIENYVHRIGRTGRSGKTGIATTFINKSCDESVLMDLKALLIEAKQKVPPVLQVLQTGDETMLDIGGERGCTFCGGLGHRITDCPKLEAMQTKQVTNIGRKDYLAHSSMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4D1</accession>
    <name>W5N4D1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Caspase activity and apoptosis inhibitor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4D1"/>
    <dbReference type="FunCoup" id="W5N4D1">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015490"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015519.1">
        <property type="protein sequence ID" value="ENSLOCP00000015490.1"/>
        <property type="gene ID" value="ENSLOCG00000012584.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RN9N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017010"/>
    <dbReference type="HOGENOM" id="CLU_078683_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4D1"/>
    <dbReference type="OMA" id="QVPEKKD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012584">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038991">
        <property type="entry name" value="CAAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14740">
        <property type="entry name" value="CASPASE ACTIVITY AND APOPTOSIS INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14740:SF3">
        <property type="entry name" value="CASPASE ACTIVITY AND APOPTOSIS INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15335">
        <property type="entry name" value="CAAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="33819" checksum="FF34BAAEA2B5ED0B" modified="2014-04-16" version="1">MTGKKSAKEKKRKRSHSEGRHVERKRRSTESSVEDLKVDIENESRETDEPSDIEEGGLNLDVPFRPISAYITDRQEMLEQCFRVLGETKLKKMLPEELKNCSFDEIKRLCLEQLEQISQRNLLQILEEDKGDEVTGNSDVEEEVSEMGKRKSETDGQQDNSVDSTSSMKENAQVEDHKQGGASGDESDALSINADTCDSDIEGHKEEPEDRKNMETAAVPVPPETGAVKPADPKKELQKDIEKSVSEILALATPNQEESKELVISSTPAVVQPSAQQLELLELEMRARAIKALMKANEVKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N502</accession>
    <name>W5N502_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">LMBR1 domain containing 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="1327"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015711"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015741.1">
        <property type="protein sequence ID" value="ENSLOCP00000015711.1"/>
        <property type="gene ID" value="ENSLOCG00000012758.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018651"/>
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    <dbReference type="InParanoid" id="W5N502"/>
    <dbReference type="OMA" id="KENCKSA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012758">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="54"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="425"/>
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    </feature>
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    </feature>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="715" mass="83144" checksum="A0FB8BAF9B204E60" modified="2014-04-16" version="1">MSGAALGIEIVVVFFLALFLLHRYGDFRKQQKMVLFGTLLAWYLCFLIVFILPLDVSTTIYKQCKNETEAHSAVTLLLANQTAGNGSISPTPSLQRKVCTKPWSYIPDGIMPIFWRVVYWTSQCLTCLYLPYLRTYCHGTPTQVAVLSRTHCFPTLQCEAFAEGTVMFLQTACMSYTVLLPSWKLQALLSEPHCLTTSRLYCTYNYISFSTSVLKIDLTQNQKGSFSIYGHMLTLFLNKAQLFVDTERSSSKTITFKMTTGDVREEVRKVSESIKYNHPLRKYIDTILRKCPTDYQEKMGRNMDDYEDFDDKQNTYPSEKSLTKLHKQVIYAVQRHNRTQVQWQILLEQAFHLEDVAKNEASATRQFVRSFAPQEPEGWVSRYLYTPTVEWYWECQLKQWFYRALAILLSAFSVAVVWSECTFFSTRPVLSLFAVFIQLAERTYNYLYIEMACFITIFFLCTCVYSTVFWIRVFNYYYLASHHQTDAYSLQFSGMLFCRLTPPLCLNFLGLIHLDSTISHQKKEQTAYTSIMGSMRVLSFIADGFYIYYPMLIVILCIATYFSLGTRCLNLLGFQQFMGDNDMTSDLIDEGKELIRREKRKRQRIEDGENRRRELKDRYGNPREDYTRNRNVNSELKETNFSDSTNVSSSRQAMKYTRSSGRPDRDRVELLQDAEPLDFNAETFSDDPLEAEPGSRHPPGGRYLSMSRSRIFDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N528</accession>
    <name>W5N528_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sperm flagellar 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015737"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015767.1">
        <property type="protein sequence ID" value="ENSLOCP00000015737.1"/>
        <property type="gene ID" value="ENSLOCG00000012789.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008160"/>
    <dbReference type="HOGENOM" id="CLU_002424_1_0_1"/>
    <dbReference type="InParanoid" id="W5N528"/>
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    <dbReference type="Proteomes" id="UP000018468">
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0097225">
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Sperm_flagellar-bone_growth"/>
    </dbReference>
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        <property type="entry name" value="KPL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="971"/>
        </location>
    </feature>
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        <location>
            <begin position="1161"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1254"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1931"/>
            <end position="1956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="850"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="895"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="945"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="958"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1170"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1181"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1264"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1300"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1998" mass="226155" checksum="913D8966C2F73879" modified="2014-04-16" version="1">MSDILCQWLNSELRISKHVEPSSFAKDFSTGYLIGEVLHKYQLQDDFDLFSKSKTADSKLNNFTRVEPTLQLLGVPFDLRIAQSIMSQQHGVATRLLYQLYIVLQKKKKAGLTGVALETLQPTALAKLQSIEREIYNDRLRGAVQREADIRLQKTSQRYELKAKEIQDKVSLVRYQEMQKMQKLQNDLRLQSIEKHRLARKRQNEIMARIQAAIVQIPKPPPRRTLKAIELRKQQKKQLEAQDVYSEILQFEKVRRKLSPSAGVTSSLSALQGSFQTDGQSTKFLSPSWEEKAQASSLYILKIRQRLEEDAAAREQREKRRRKVLVEQFRALEAQEETYREEQLVNRLTKQSQQERRLAVQLMQVRHEKEVVRRNRIFREKQYEQQRLKEFQEALDREAALAKQAQIDHMEEIRKEIELHDKIAAERAEARYRKHYSVCQEVLSQMVDLVTKVGEYRELTNNLIPAKLMQEWKELFYNGKPLYEQASVDPLPPEPTPEQILELEKMAILDNQDYEDYQHMTGEWAAPDDCEVKEPLPKSNILGHVVYRLLNIINPPQPTSPSPHFPPFTLKACVLGKLFSGKTSCLEKIAKAYHIQILSANTMIREAVNAFQDNEMAEALPELSNSGKERELSARAQLGAVVEKILKKGKSVPDELLVDIIVEAIRHVPAETGWILDGFPVDINQAKLLEKALSGTDPDKAGSKKSKAKSSSIVVDPNAAKEPPRPSPALDLALLLEVSDDLVLDRAAKKTYTGGSGPGQEVYHPSQEGPLTNVGVKDQFVPIRDLSLEKRQIQHRITAFHDTWPKLEKWFALQQNILVKVTAEEDEETVFKKVESVLLEAMISKNKNGKEIVQEDKPSIESPVSDVPPPAPVPETKPSATVVATEGMEVKPSRSKSATRSSKGSRSRSSSAKDRKGRKASIPEGKEVTKKVSKSGSGSAQGKTTRASESSTSEMAPQPAPTVSPSPKPGSDKWVYVDELLPKEIPEYLVPYWENVCDSYITNVKMVMRNLRSEGYLIIHHLYDIREEFKEFLRRPDHKQEFVSQWQQDYNSIPDDMRDDEETKAELHQRLDDLRERLWDICDNRREEAMQERGNVISGGWLEDHTAILINHFSSLMQVEVDRCQDTLRLLRDYYKGMEGGLLPEASMDFACIPLVDTVEEDPKHDTERTMSASGLANSERQVKSAGQKENEAEDKKPKTEKIPLISRRPLSSDPSGTKLKTGLADLKSKDEQLVMDIWQAAWTAISNMMSAEIQQKEAEEEEERLRSAEREQHRASQVSAMLSAGQEKKKSPKKKGAPSPVPQHTPPPAPEEDAGELRKRAVKLRIRQEYRAALEHEESAVKVRLEQIKSKSLAVVRDLLQKADNAYKEMEEWLGARFLAEMTSIDRLTDVARHHIESSAQIGNELVLSCSDFFVDGDLKVVPSPEPAPRPPPLETPMNSTLTIAQLHTFHCQLCTAAPAGVLSSRDFFDMLQDLTSLKLGSDLLPDPWLQLSGAQIQELTSLLTLDSELIDWRKFLLLAAQPWPYPSQGDLLHTLARFKALDPDSTGFITEEQFDQTELWFDSQTELPVPEDPTEPLPFNRMANLKKFFFVLFADRQAPVARLDYVNMLLYFASHPDPVQGVHRAMSIATGERISQRKTSSRLLQSVLQIDEDVDIKVPEAGTEEDPGAEEVGGVTVTALLRVVCHGGVMMENQNRFRSHGKTKEEYKEDFCRIYKELGYEDQDKIPFQVLSQHPFMLDLIENSIQYKLVVANKVHEMQTSGWSAEMAQHRRRACTSQIQRNCGRFTESHSSGCLLGVLCPVSPLEVGRRTAAWEEQGCLLCGFQASQGVVRRASLTQLDASPSVICTSVLLTGQLAHCTRRQLEDKLRSGEICLSSWPVSKAPAMQTQAPEESRPQLVASLAPQSPSQGYFSRITDLQHKLYTGQSPPRLLPWRQSTPASPGPGQPIKCHSADWRRDPSPVGSWFCGSPEPAALFPPLLSTWMQRDARSRPFRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5K2</accession>
    <name>W5N5K2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5K2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015911"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015941.1">
        <property type="protein sequence ID" value="ENSLOCP00000015911.1"/>
        <property type="gene ID" value="ENSLOCG00000012923.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159235"/>
    <dbReference type="HOGENOM" id="CLU_055524_0_2_1"/>
    <dbReference type="InParanoid" id="W5N5K2"/>
    <dbReference type="OMA" id="QRMNHCC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012923">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03159">
        <property type="entry name" value="TM4SF9_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000001">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF261">
        <property type="entry name" value="TETRASPANIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="288" mass="32661" checksum="05D17CAE8FF0E08E" modified="2014-04-16" version="1">STVNWDKMHYYRYENAEVSCCYKYLMFSYNIIFWLAGAAFIAIGFWAWSEKGVLLDLTQVTRIHGFDPVWVVLVVGGVTFILGFAGCVGALRENICLLKFFSGLIGFIFFLELTAAVLAFVFQDPLRDWLSDFFLNNIKGYREDIDLQNLIDSLQRLNNCCGAQGPNDWNGNVYFNCTTENPSREKCGVPFSCCLSDPADMVVNTQCGYDIRSKKENIWSTAIYTKGCIPALEAWLPRNIYIVAGVFIVISLLQMLGIFLARTLIGDIEYTYCNFKVICNLRAAAHWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5N664</accession>
    <name>W5N664_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkeyp-55f12.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069049441.1">
        <property type="nucleotide sequence ID" value="XM_069193340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N664"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016153.1">
        <property type="protein sequence ID" value="ENSLOCP00000016123.1"/>
        <property type="gene ID" value="ENSLOCG00000013091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="138241030"/>
    <dbReference type="eggNOG" id="ENOG502TM0F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173241"/>
    <dbReference type="HOGENOM" id="CLU_2276471_0_0_1"/>
    <dbReference type="InParanoid" id="W5N664"/>
    <dbReference type="OMA" id="ENAREEH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013091">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027893">
        <property type="entry name" value="GON7_meta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15387">
        <property type="entry name" value="DUF4611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="104" mass="11411" checksum="80530950984DFD56" modified="2014-04-16" version="1">MAVCELRGDLKFRDGSTKEFVVKAENNLKSVLSGLQKIRTEVSDVLSDLVSQEKGVTVNSQADNGSLDDEGDEESDEEEEVEIKTKSKGVRVEPPAKRTKTLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6B4</accession>
    <name>W5N6B4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6B4"/>
    <dbReference type="FunCoup" id="W5N6B4">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016173"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016203.1">
        <property type="protein sequence ID" value="ENSLOCP00000016173.1"/>
        <property type="gene ID" value="ENSLOCG00000013130.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163871"/>
    <dbReference type="HOGENOM" id="CLU_019722_1_0_1"/>
    <dbReference type="InParanoid" id="W5N6B4"/>
    <dbReference type="OMA" id="GLATWFK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013130">
        <property type="expression patterns" value="Expressed in larva and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF2">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56422" checksum="F90C06A2C969EA98" modified="2014-04-16" version="1">MWLLLFAAGFLILGFALYSRLFANSASPFSRDAVRPPAPLVTDQKQRDKVLKQGFSADRVPAELDAVVIGSGIGGLTAAAVLAKAGKRVLVLEQHDQAGGCCHSFVDKGFEFDVGKLDRHYLNEDKEITFLHLHVGFPNKSHFLTTDLWNMCCVIAELIVFCPVLAGVPPKDSSFLMNTLLLHHYKRGAYYPRGGASEIAFHIIPVIQKAGGAVLVRAPVQRILVDQKGSACGVTVKKGQEEVTVLAPVVISDAGMFNTFEKLLPPEIQAKPEIQSRLSMVQHGMGSFLVFVGLDGTKEDLGIVSTNFWMYKHNDLDELMGRYASLSREEVADNIPMMFITFPSAKDPTAKIRHPGKSCMTLLTMAKYEWFEEWKDSTLKKRGPDYGSLKMGIANALLGWALEIFPQLRDKVVFMEAATPLSNMHYLGSPRGEMYGAEHDLARFTPEVVAQTRAATPVKKLYLTGQDVFCNGLAGALHGGLLCASAVLDHIVYIDLLALKKRLKWEKARKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6C2</accession>
    <name>W5N6C2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peripheral myelin protein 22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Might be involved in growth regulation, and in myelinization in the peripheral nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635249.1">
        <property type="nucleotide sequence ID" value="XM_006635186.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212131.1">
        <property type="nucleotide sequence ID" value="XM_015356645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6C2"/>
    <dbReference type="FunCoup" id="W5N6C2">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016181"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016211.1">
        <property type="protein sequence ID" value="ENSLOCP00000016181.1"/>
        <property type="gene ID" value="ENSLOCG00000013140.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695156"/>
    <dbReference type="KEGG" id="loc:102695156"/>
    <dbReference type="CTD" id="678607"/>
    <dbReference type="eggNOG" id="ENOG502S0F5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182696"/>
    <dbReference type="HOGENOM" id="CLU_138632_1_0_1"/>
    <dbReference type="InParanoid" id="W5N6C2"/>
    <dbReference type="OMA" id="HTADLWQ"/>
    <dbReference type="OrthoDB" id="6084046at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013140">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000019">
        <property type="entry name" value="Peripheral myelin protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF7">
        <property type="entry name" value="PERIPHERAL MYELIN PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01222">
        <property type="entry name" value="PMP22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867257" description="Peripheral myelin protein 22" evidence="12">
        <location>
            <begin position="30"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="17361" checksum="CCB1251889F73872" modified="2014-04-16" version="1">MLLLLLGIIILHIAALVLLFVSTIVSAWAYDGNTSSDLWSNCTTTNGGSHCDQAFTGEWIQAVQALMILSIIFSVLSLFLFFCQLFTLQKGGRFFLTGIFQILASLFVMSGAIIYTVKSTEWLSQAATYGFAYILAWVAFPLALISGLIYVILRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6U7</accession>
    <name>W5N6U7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">2',5'-phosphodiesterase 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial deadenylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029309">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029310">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6U7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016356"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016386.1">
        <property type="protein sequence ID" value="ENSLOCP00000016356.1"/>
        <property type="gene ID" value="ENSLOCG00000013277.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694543"/>
    <dbReference type="KEGG" id="loc:102694543"/>
    <dbReference type="CTD" id="201626"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157205"/>
    <dbReference type="HOGENOM" id="CLU_016428_7_2_1"/>
    <dbReference type="InParanoid" id="W5N6U7"/>
    <dbReference type="OMA" id="FRLKSAC"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013277">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000018">
        <property type="entry name" value="2',5'-phosphodiesterase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="16">
        <location>
            <begin position="151"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="277"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="589" mass="66090" checksum="D5F7FBEF60C247DB" modified="2014-04-16" version="1">MYRCLACISHRFSKTLSPYWFGTGESVWGPGNRFGRRFFGRMERAVVRCVPWETKITISFTLDGSQKHMLRDQTEELGKALSRIANSVIKGQAKEKRSKKKKSELTEVQEPVVKLYYMDDFVAEDALNADAWQDGAVLHVGDSRYKVERNPPAFITAELPKSLLAGFPVCTKLVIEFGELKHCQFTWYKEKAPNAEKDGVENCWIEAGNERVFTPSNADIGLKLKLKCTPGNGERFGVSKEVVTPGPVEAGPGSCTFDNRHLYTNKVTDESTIRAVSYNILADIYAQTELSKTVLYPYCAPYALEIDYRQSLIKKELAGYNADIVCLQEVDKCVFADSLSPALDAFDMDGVFRLKEKQHEGLATYFRRSKFELLSQHDITLSEALLSDPLHRELLERVSTNPVLKENVIQRSTTLQVTVLQSVKDVSRKLCVANTHLYWHPKGGHIRLIQMAIALRHLSWVTSGPHPGIPLLFSGDFNSTPSTGMYQLVTQGAVPESHEDWASNGEEELCPLAVSNPFQLQSACGEPAYTNYVGGFHGCLDYIFIEPRALQVEQVIPMPSHQEVTTHQALPSVLHPSDHIALVCDIKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7L3</accession>
    <name>W5N7L3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sphingosine kinase</fullName>
            <ecNumber evidence="7">2.7.1.91</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.91" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01000478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7L3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016622"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016652.1">
        <property type="protein sequence ID" value="ENSLOCP00000016622.1"/>
        <property type="gene ID" value="ENSLOCG00000013477.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157864"/>
    <dbReference type="HOGENOM" id="CLU_013399_1_1_1"/>
    <dbReference type="InParanoid" id="W5N7L3"/>
    <dbReference type="OMA" id="VKYQVIM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013477">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008481">
        <property type="term" value="F:sphingosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000005">
        <property type="entry name" value="Sphingosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF47">
        <property type="entry name" value="SPHINGOSINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="8">
        <location>
            <begin position="147"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="465" mass="50292" checksum="F2368F536EE4F45D" modified="2014-04-16" version="1">TEPDPPQRNGSPRAQLHGEFTAAAAGKQVRYAVSLTDRELAVQKITSAPAGRNKVLLNLKDCIGCRAFKGADSAEAGAYLSVVFYPFKKKWMGAGLARHRVEYCFEVALSQDARTNRDEAERWASAIRESCVRLWPQGQGVLGFSVPRPSRILVLLNPHSGKGQALSLFTSHIQGMLTEAGIAHQLVITERQNHARELVREADLSQWDALVIMSGDGLLFEVVNGLMERGDWEAAIRTPLGILPGGSGNALAASINHYCGFPPAMGEELVVSCGFQLCRGLVSRLDLAAVHLGSGQRLFSFLSLAWGFVADVDIESEKYRRVGAARFTLGTLVRLACPPDPLLVPLAQPVPGCWTAVPEEDFVLLLAMYQSHLAEDLLAAPADGLLHLFYVRAGVSRAALLRLFLAMERGAHLAAGCPQLVHVRARAFRLEPLSPKGMITVDGEAVEYGPVQVQVHSGLGRLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N826</accession>
    <name>W5N826_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Plastin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">T-plastin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Actin-bundling protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N826"/>
    <dbReference type="FunCoup" id="W5N826">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016785"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016815.1">
        <property type="protein sequence ID" value="ENSLOCP00000016785.1"/>
        <property type="gene ID" value="ENSLOCG00000013613.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183097"/>
    <dbReference type="HOGENOM" id="CLU_015284_2_0_1"/>
    <dbReference type="InParanoid" id="W5N826"/>
    <dbReference type="OMA" id="TVNHLYV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013613">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039019">
        <property type="term" value="P:pronephric nephron development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072114">
        <property type="term" value="P:pronephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="CH_PLS3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21334">
        <property type="entry name" value="CH_PLS3_rpt4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CH_PLS_rpt1"/>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Plastin 1"/>
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    </dbReference>
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        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Plastin-3 isoform 1"/>
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        <property type="entry name" value="Plastin-3 isoform 1"/>
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        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
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        <property type="entry name" value="CH_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLASTIN-3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="EF-hand_7"/>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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    </dbReference>
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        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACTININ_2"/>
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        <property type="entry name" value="CH"/>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="151"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="295"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="425"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="546"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="658" mass="73919" checksum="0CF9A77C9411843B" modified="2014-04-16" version="1">MEIVPYPAVLRAACFQKTGNSLGAEPEGSQREMAGKISKDELEELREAFEKVDLNGNGYICDYELHELFKEANLPLPGYKVREIIQRLMEEGDKDKDNRISFDEFVSIFQELKSSDIAKSFRKAINRKEGILAIGGTSELSSEGTQHSFSEEERFAFVNWVNKALENDPDCKHVVPMDPNSNSLFKAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTTQENLNLALNSASAIGCHVVNIGAQDLREGKPHLVLGLLWQIIKIGLFADIELSKNEALAALLRDGETLEDLMKLSPEELLLRWANFHLENAGWNKINNFSSDIKDSRAYFHLLNQIAPKGTKEGETRIDINMSGFNEKDDLKRAELMLQEAEKLGCRQFVTPSDVVSGNPKLNLAFVANLFNKYPALIKPENQDIDWGLLEGETREERTFRNWMNSLGVNPHVNHLFADLQDALVILQLYENIKVRVDWNRVNKPPYPKIGTNMKKLENCNYAVDLGKKDAKFSLVGIGGQDLNDGNPTLTLAVVWQLMRRYTLNVLEHLGDGEKATDEIIVKWVNKALSEAGKKTKIQSFKDKEISSSLPVLDLIDAIQPSSINYDLVKTGSLSEEDKLENAKYAISMARKIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5N829</accession>
    <name>W5N829_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Disintegrin and metalloproteinase domain-containing protein 10</fullName>
            <ecNumber evidence="4">3.4.24.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Kuzbanian protein homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase of broad specificity.</text>
            <dbReference type="EC" id="3.4.24.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.81" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01024871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N829"/>
    <dbReference type="FunCoup" id="W5N829">
        <property type="interactions" value="1378"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016788"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016818.1">
        <property type="protein sequence ID" value="ENSLOCP00000016788.1"/>
        <property type="gene ID" value="ENSLOCG00000013609.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689715"/>
    <dbReference type="KEGG" id="loc:102689715"/>
    <dbReference type="CTD" id="566044"/>
    <dbReference type="eggNOG" id="KOG3658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164516"/>
    <dbReference type="HOGENOM" id="CLU_004602_0_0_1"/>
    <dbReference type="InParanoid" id="W5N829"/>
    <dbReference type="OMA" id="MAVFIRC"/>
    <dbReference type="OrthoDB" id="2149267at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013609">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902945">
        <property type="term" value="F:metalloendopeptidase activity involved in amyloid precursor protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04270">
        <property type="entry name" value="ZnMc_TACE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000011">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000002">
        <property type="entry name" value="disintegrin and metalloproteinase domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034025">
        <property type="entry name" value="ADAM10_ADAM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049038">
        <property type="entry name" value="ADAM10_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF4">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21299">
        <property type="entry name" value="ADAM10_Cys-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13574">
        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="11 26" id="KW-0732">Signal</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867371" description="Disintegrin and metalloproteinase domain-containing protein 10" evidence="26">
        <location>
            <begin position="24"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="684"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="28">
        <location>
            <begin position="228"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="27">
        <location>
            <begin position="465"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="712"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="active site" evidence="23">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="757" mass="85242" checksum="B2E8BA24082FE69C" modified="2014-04-16" version="1">MSLIDMFLIKFAVFLYCARNAGGHYGNPLNKYIRHYEGLSYDTDVLHQKHQRAKRALSHEERFVHLDFHAHGRHFNLRMKRDASMFTQDFKVEFAGEELDYDTSHIYTGELYGEKGTMSHGSIVDGKFEGFIQTHQGTFYVEPSERYIKDNAVPFHSVIYHEDDIDYPHKYGAEGGCADHTVFERMKKYQASAVEKTPKDDHIEEEEEGQSSGPVLLRSRRAAGADKNTCQLFIQTDHLFYKYYGTREAVIAQISSHVKAIDSIYQTTDFQGIRNISFMVKRIKINTTEAERDKSNPFRFANIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPSGSSGGICEKSKLYSDGKKKSLNTGIITVQNYASHVPPKVSHITFAHEVGHNFGSPHDSGTECTPGESKSQDLKEKGNYIMYARATSGDKLNNNKFSICSIRNISQVLEKKRGNCFVESGQPICGNGLVENGEQCDCGYSDQCNDSCCYHANEPDHLKCKLKPNKKCSPSQGPCCNPDCTFRSNIQKCREESECAHQGFCNGNTAQCPTSEPKANFTACHGETQVCLNGQCTGSICEKYGLEVCTCASGDGKDDTELCHVCCMEKMRPDTCSSTGSDQWSKFFNKKVTTLQPGSPCNDFRGYCDVFMKCRLVDADGPLARLKKAIFNPELYENIAEWIVAHWWAVLLMGIALIMLMAGFIKICSVHTPSSNPKLPPPKPLPGTLKRRRPQQANPQPQRQRQPRENYQMGQMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N860</accession>
    <name>W5N860_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Endonuclease 8-like 3</fullName>
            <ecNumber evidence="4">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DNA glycosylase/AP lyase Neil3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Endonuclease VIII-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Nei-like protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01002114">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630151.1">
        <property type="nucleotide sequence ID" value="XM_006630088.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N860"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016819"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016849.1">
        <property type="protein sequence ID" value="ENSLOCP00000016819.1"/>
        <property type="gene ID" value="ENSLOCG00000013638.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689381"/>
    <dbReference type="KEGG" id="loc:102689381"/>
    <dbReference type="CTD" id="55247"/>
    <dbReference type="eggNOG" id="ENOG502QWRN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153230"/>
    <dbReference type="HOGENOM" id="CLU_482283_0_0_1"/>
    <dbReference type="InParanoid" id="W5N860"/>
    <dbReference type="OMA" id="GMKGSVM"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013638">
        <property type="expression patterns" value="Expressed in ovary and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08969">
        <property type="entry name" value="MeNeil3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000008">
        <property type="entry name" value="Nei-like DNA glycosylase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="entry name" value="MutM-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015887">
        <property type="entry name" value="DNA_glyclase_Znf_dom_DNA_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="H2TH_FPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF10">
        <property type="entry name" value="ENDONUCLEASE 8-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01242">
        <property type="entry name" value="ZF_FPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="27">
        <location>
            <begin position="2"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="FPG-type" evidence="26">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="25">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="28">
        <location>
            <begin position="485"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="28">
        <location>
            <begin position="532"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="579" mass="65107" checksum="C2728B2973976424" modified="2014-04-16" version="1">MVEGPGCTINGEKIRSRVQKGQKVKEIKGGATLQNGPHAGSLQSFTGCQFTGVETLGKELFMYFGLRALRVHFGMNGSLRINPSERQDRGGMPSALEVSLTKDTVCFFESTVEIRLTADCQQKVRAFESLDVCSPKFSFSRAEDEIRKQRNDILCDVLLDQNVLPGVGNIIKNEALFDSGLHPAVKVSQLTELQIRHLVKMTRDFTLLFYKCRKSGSPLYKHYKVYKRPTCGQCNGRITVCRLGENRRMTYFCHHCQNQDPGQVDVNKLPVRNSLVGLVYQGRMQSNDHIAKKEEEEWTCELCTLINQPSAKSCNACLTPRPKLSKVEINEEFSSFTRDLIKFPHNAFAKPQEEIKLNRKTVFGTSTLVITDFSRKPSPLTSPQLNSEKSNLNPLVTHSSLNKHSFIQGKTGPNYTLTGSSSKSSEPSGREYASSFHQPTKKMKMDRSSFLSNNAQTWNSNSVFSEPGVNKTDGSAPASHGKPCCTTHRQQCALRVVKKEGENKGRQFFTCSLPRETQCGFFEWADLHFPTCHHGKRCLMRTVLKLGPNNGRDFYVCPLGKTKQCDFFQWVENSPADRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N989</accession>
    <name>W5N989_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ARF like GTPase 16</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000587">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000588">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N989"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017198"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017229.1">
        <property type="protein sequence ID" value="ENSLOCP00000017198.1"/>
        <property type="gene ID" value="ENSLOCG00000013950.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111327"/>
    <dbReference type="HOGENOM" id="CLU_040729_14_1_1"/>
    <dbReference type="InParanoid" id="W5N989"/>
    <dbReference type="OMA" id="ETTFSMP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013950">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46688">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46688:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="176" mass="19346" checksum="9BFCB01701F89314" modified="2014-04-16" version="1">GVEMCLLLGATGVGKTLLLKRLQNLSLRDHHSDVGEPRPGQHRVGTNLTDLMLHKRKLTVRELGGCMGPIWSSYYRDCSSVIFMIDAANTCQVSASCVQLLSVLSAEPLQNAAVLILFNKLDLPCPMSLVEMKSLFRLDDIITCASQPITVQEVSARSGRGLQDVLQWLESHRPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9N2</accession>
    <name>W5N9N2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01024793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017341"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017372.1">
        <property type="protein sequence ID" value="ENSLOCP00000017341.1"/>
        <property type="gene ID" value="ENSLOCG00000014067.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160363"/>
    <dbReference type="HOGENOM" id="CLU_001731_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9N2"/>
    <dbReference type="OMA" id="GEEVIWC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014067">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000753">
        <property type="entry name" value="Leucine rich repeat kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000361">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000091">
        <property type="entry name" value="leucine-rich repeat serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032171">
        <property type="entry name" value="COR-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF81">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Roc" evidence="16">
        <location>
            <begin position="606"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1218"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1771"/>
            <end position="1791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1991" mass="223738" checksum="7270F7AF68CBAC40" modified="2014-04-16" version="1">MDEHDGTVDMGSKQSILGSTALSQSPTIDNIQAAYRAGESRQARELIRLSCGETSRAQLEKLLSLAAGSGDLESVQYLLKEVRVQVPKQPSEDNPAVLAAYFGHEHVVKELLDSIPGPCVQKDLLNWMLATACQQGHLEVVKLLVLTYQADAENCAIRTHDFAVITGLPLYAAARAGNEEISTFLLQNGAVFTSYTLMDHPAFFKQLLRKHYIEPSDPECSGELSDALSVRWAQLKLPWLDLDWLMDISCRITQLDLSSNSLSDLPSVVPWGLIHLQRLDLSDNQLKDLPAGRSSKEIICTWLREINLSQNEMVSLPSGLLHLKSLQKLIAAKNKLVMLFDVPNETNWIGLRKLQELDVSDNQLATLPCPTLHCFKSLISLNVSRNKLKEFPDPWACPLKYCKASSNCIENLPDTISIFWKNHLKEVDFSENNLKQLPSYIFQLEGLVSLKVSGNQLCTLPSPNKWTCSQLKTLDLSKNQLGKTDEGVRTRKLAFFTTWNRRDPDSVFFSVCSIEFPGVLRDCLEVLFLNDNQLESVPQSVCSLKNLTELYLSNNPGIRELPHELGQLSNLWQLDIEDLNISNVPVEVRNEGPAAVLGFLRAHLRKAEKCRLLKMIVVGPPRQGKTTLLETLQTGRVTQFLPTECSIRTSTWTLDRPSGVKSHTDSVEFNVWDIGGPASMSTVNQCFFTDKALYVVIWNLALGEEAVANLQSWLLNIEARAPSAAVVVVGTHLDLIDTKFRTERIATLRAYILALCRSPSGARATGYPDITWKHLHEVSCKNLEGLDGLRRLIFQVACSMKDMTSSASSQKLVGRLIPKSYLLLQDAVITERQRRDLADEVQYLTDLQLENIIEQNPDSDIKDYEDLQTAISYLIETGNLLHFPDTSHGLCNLYFLDAVWLSECLERIIHIKSSKSVARNGVIKAEDLRMLLVGTGFTEQSEEQYFQFLAKFEIALPVASNSYLLPHLLPPKPGLDIHGLRQQTTNTIQRLFKMSFVPAGFWERFIARMLISLNEMDVQSFENRKNTKTLRNRRTSIYSFAGNQQRNRCSTFRVRRSQTIYWKEGLLVTFDGGYLSVESSDLNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTASENDGSMLIEQYAPCPSCATVTREEEEPPVKAKGTHYFNMEDCVIAAVEQEHIACPNHPAQPVPLQELVPELFMTDFPARLFLENSDLEYSEEEHNVLGQGGSGTIIYKAKYKGLPVAVKRFQFKKCRQQSLTSSTDTMVKHLQSMDALRNFSEFRQEASMLHSLQHPCIVSLIGISIHPLCFALQLAPMGSLNTVLEEHSKAGVQFLPLGHMLTYRVAYQIAAGLAYLHRKNIIFCDLKSDNILVWSLEVSDPVNIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPGIVYDEKVDMFSYGMVLYELLSGLRPVLGQHQLQIAKKLSKGVRPVLGIPEEVQFHCLQTLMLECWDTKPEKRPTAMALLRLMQEPSFPCFKYLLPCGKHSQLFLSSEQGHPAVFWDIDQDERNYTVVNVDKGQVEVRRMACPGMKISCQMKVENTLWTATEDQNIYIYSLKDMCPLNNPQQHFFCPAVVTCLFIVPARKESLPLVFVGMADGLIAVYTLINDVPHDGESYLCSHTLNRSVFSIADEDPRQRPYPVKAMLLANSGTEVWYTNGPGILIIDCLTLQAVNRLDPYTYPSTIVSMASNSGCWGEEAAWCLDDHTNTLLMYHAASYQLCARYFCGDRNPLRDMFVVQQPAGAVSPSPPTPEKSSEHSSPGEVTIMYSEETGTQIIMHQDSLTDYCSMSSASSEQLCQMDRSSSGVLSSLASSCSMPFSTDCEDSDRAQELPVDPTSPETFEALLESETPEHLQAVRVLAVRNTLWIPRRGGEVMVIKLEKQAEVQRGRVIAVLRAPTLPEHGVLMEAAVVAKDTVVCGFRNENMEWCLAVWRGWGGREFEIFYQSYEELGRLENSMRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9T9</accession>
    <name>W5N9T9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tuberoinfundibular peptide of 39 residues</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Parathyroid hormone 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022184">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206682.1">
        <property type="nucleotide sequence ID" value="XM_015351196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9T9"/>
    <dbReference type="FunCoup" id="W5N9T9">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017398"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017429.1">
        <property type="protein sequence ID" value="ENSLOCP00000017398.1"/>
        <property type="gene ID" value="ENSLOCG00000014124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695008"/>
    <dbReference type="KEGG" id="loc:102695008"/>
    <dbReference type="CTD" id="113091"/>
    <dbReference type="eggNOG" id="ENOG502S3PJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000026259"/>
    <dbReference type="HOGENOM" id="CLU_1677252_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9T9"/>
    <dbReference type="OMA" id="MMSAPDF"/>
    <dbReference type="OrthoDB" id="9940245at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014124">
        <property type="expression patterns" value="Expressed in brain"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029396">
        <property type="entry name" value="TIP39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28585">
        <property type="entry name" value="TUBEROINFUNDIBULAR PEPTIDE OF 39 RESIDUES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28585:SF1">
        <property type="entry name" value="TUBEROINFUNDIBULAR PEPTIDE OF 39 RESIDUES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14980">
        <property type="entry name" value="TIP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="167" mass="19114" checksum="9BF814B9190E2D64" modified="2014-04-16" version="1">MKPVSPPRSAFLFLALVVSTLLPSGYTLPRLRSLRSTRLAPPRIGQDDSSRFRPQLEVLYPSVSLRDWSVQMMSSPGISDPQPKSLRLPGRVWLPFSQSEMESLAQEEDLNKSWPLPWSQQMANEEKRNIVVADDAAFREKSKLLTAMERQKWLNSYMQKLLVVNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAS5</accession>
    <name>W5NAS5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 20</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014909">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAS5"/>
    <dbReference type="FunCoup" id="W5NAS5">
        <property type="interactions" value="1603"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017734"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017766.1">
        <property type="protein sequence ID" value="ENSLOCP00000017734.1"/>
        <property type="gene ID" value="ENSLOCG00000014407.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157863"/>
    <dbReference type="HOGENOM" id="CLU_015166_16_0_1"/>
    <dbReference type="InParanoid" id="W5NAS5"/>
    <dbReference type="OMA" id="NWAVGIP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014407">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000051">
        <property type="entry name" value="Mitochondrial carnitine/acylcarnitine carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF56">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE_ACYLCARNITINE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="112"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="211"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="305" mass="33407" checksum="8C83794B882FE765" modified="2014-04-16" version="1">MSKQQQPISPLKNFFAGGFGGVCLVFAGHPLDTIKTVRLPLHSGPIRSGFNYLQGSSIQNWARTGCKRFRGVRGLYKGMAAPIVGVTPMFAVCFFGFGLGKKLQQRNPDDVLTYPQLFAAGMLSGVFTTAIMAPGERIKCLLQIQAASGEVKYAGPLDCVKQLYRESGLRGVYKGTVLTLMRDVPASGMYFMTYEWLKHILTPEGKSPNELSVPSVLFAGGMAGIFNWAVAIPPDVLKSRYQTAPEGRYPHGFRDVLRELLREEGVASLYKGFTAVMLRAFPANAACFLGFEVAMKFLNWIAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAT5</accession>
    <name>W5NAT5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myocyte enhancer factor 2aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01005030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015199078.1">
        <property type="nucleotide sequence ID" value="XM_015343592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAT5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017744"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017776.1">
        <property type="protein sequence ID" value="ENSLOCP00000017744.1"/>
        <property type="gene ID" value="ENSLOCG00000014408.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684314"/>
    <dbReference type="CTD" id="30564"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156205"/>
    <dbReference type="InParanoid" id="W5NAT5"/>
    <dbReference type="OMA" id="MNTQRIS"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014408">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF637">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="220"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="418"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="464"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="521" mass="55531" checksum="2BFBBC622E054500" modified="2014-04-16" version="1">MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVEKLRNKGLSDCASPEPDDCFGHSPTMDERYGKLNEESDLIYKRCGALNKKEHRGCDSPDPDASYVLTPHTEEKYKKINEEFDNMMRNHKIPTALPQQNFSMHVAVPVTNPNTLSYSNPGGSLGTPALAAATASLSDGGMLSPPQGSLHRSVVSPGAPQRPSSTGSAGGMLGTTDLTVPNGAGSSPVGNGFVNPRGSPGLLGTPSGNGLGKVMPTKSPPPPGGNLGIGNRKPDLRVVIPPSSKGMMPPLNTQRISSSQAAQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLSSAELSALQGFSSPGGLALGSMSAWQQHQLGQAALSSLVSGGHMPQGPNLSINTSQNVHIKSEPISPPRDRFPAQPPPPARPDMGRSPVDSLSSSSSSYDGSDREDHRADFHSPLGLGGPAGGGEERESPSVKRMRMDTWVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB00</accession>
    <name>W5NB00_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat domain 46b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015586">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632799.1">
        <property type="nucleotide sequence ID" value="XM_006632736.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB00"/>
    <dbReference type="FunCoup" id="W5NB00">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017809"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017841.1">
        <property type="protein sequence ID" value="ENSLOCP00000017809.1"/>
        <property type="gene ID" value="ENSLOCG00000014469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699027"/>
    <dbReference type="KEGG" id="loc:102699027"/>
    <dbReference type="eggNOG" id="KOG0508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157094"/>
    <dbReference type="HOGENOM" id="CLU_084801_0_0_1"/>
    <dbReference type="InParanoid" id="W5NB00"/>
    <dbReference type="OMA" id="EYQGNTA"/>
    <dbReference type="OrthoDB" id="21416at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014469">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="228" mass="25266" checksum="1D9F30B092745909" modified="2014-04-16" version="1">MSYVFINDSSQTNVPLLQACIDGDLNYSKRLLESGFDPNIRDNRGRTGLHLAAARGNVEICRLLHKFGADLLATDYQGNTALHLCGHVDTIQFLVSNGLKIDICNHNGATPLVLAKRRGVNKDAIRLLEGLEEQEVKGFNRGAHSKLESMQLAESESAMESHSLLNPNLQHSEGVLSSFRTTWQEFVEDLGFWRVLLLIVVIALLSLGIAYYVSGVLPFVENQPELVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBC6</accession>
    <name>W5NBC6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cholinergic receptor, nicotinic, alpha 7a (neuronal)</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004982">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004983">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004985">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBC6"/>
    <dbReference type="FunCoup" id="W5NBC6">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017935"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017967.1">
        <property type="protein sequence ID" value="ENSLOCP00000017935.1"/>
        <property type="gene ID" value="ENSLOCG00000014571.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3646">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154617"/>
    <dbReference type="HOGENOM" id="CLU_018074_0_3_1"/>
    <dbReference type="InParanoid" id="W5NBC6"/>
    <dbReference type="OMA" id="PWILCMS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014571">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005231">
        <property type="term" value="F:excitatory extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904315">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000011">
        <property type="entry name" value="neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000009">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 14" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022250089" evidence="14">
        <location>
            <begin position="23"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="15">
        <location>
            <begin position="27"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="16">
        <location>
            <begin position="237"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="486" mass="55375" checksum="AABAD38F369C0E2D" modified="2014-04-16" version="1">MGTWGMLLHLTCLACWLDVSLQGEFQRKLYKDLMKNYSPLERPVSNDSHPLTVYFSLSLMQIMDVDEKNQVFTTNIWLQLYWNDFYLQWNASDYPGVKNVRFPDEQIWKPDVLLYNSADERFDATFHTNVLVNSTGYCQYIPPGIFKSTCYIDVRWFPFDVQRCDLKFGSWTYGGWSLDLQMLEADTSEYIPNGEWDLVGVQGRRNERFYECCEEPYPDVTFTVVMRRRTLYYGLNLLIPCVLISTLALLVFLLPADSGEKISLVCNVNIGILTFLKINLNIVTAASLRSHLWPQYFASTMIIVGLSVIVTVWVLQFHHHDPDGGKMPKWTRTILLNWCAWFLRMRRPGEEKVRSACHHSQQRGSVCSVELGANGGLAYLGTRGLEGARYGAAAEPGLGVAEEDLLLPGAHGVEHELAKILEEVRYISGRFRAQDEDGSICSEWKFAASVIDRLCLMAFSVFTILCTIGILMSAPNFVEAVSKDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBJ7</accession>
    <name>W5NBJ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-A2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000014612.1"/>
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        <property type="component" value="Linkage group LG1"/>
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        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
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    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
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        <property type="entry name" value="TIG1_plexin"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1899" mass="211738" checksum="86871061D1A5D330" modified="2014-04-16" version="1">PQKPATMALGGLALAFCLFLQAGPLEGFPVEPTGLPPDAGRFRVADTTLTHLTVHRQTGEVFVGAVNWIYKLSDNLTELRSHQTGPVDDNARCYPPPSVRACAHRLAPSDNVNKMLLVDYRGRRLVACGTIWQGVCQFLRLEDLFKLGEPHHRKEHYLSGAREADGMAGVVVEEEEEGGAGEGAVGKEAKAGSRLFIGAAIDGKSEYFPTLSSRKLVVNEESVSMFSLVYQDEFVSSQIKIPSDTLSLYPAFDIYYVYGFASRSYIYFLTLQLDTQLTQMDATGEKFFTSKIVRMCADDTEFYSYVEFPLGCTKDGVEYRLVQAAYKHRPGRRLARALGLSEKEDVLFVLFSQGQKNRANPPRETVLCLFTLRQVNEAMRERIKSCYRGEGKLSLPWLLNKELPCINTPKQIGDDFCGLVLNQPLGGLHVIEGVPLFDDRTEGMAAVAAYTYGEHSVVFVGTRSGQLKKIRVDDIPPPAQNALLYETVPVVEGSPILRDMVFSPDYQYIYLLSNKQVTRVPVESCGQYRSCRACLGSGDPHCGWCVLHNKQSSAQLACDRAHCRSPLLVSGELPVIGSPFPQTPTSVLFQTFSVCAATVGIFEDLVESEGEVLAKGQVRCTSPSPRDIPSLTQGHGDKRVVRLALRSKETGQKFVSTDFVFYNCSVLESCLSCVSSAFPCHWCKYRHICTNNLANCSFQEGRVSAIQGCPQILQTNDILVPAGMVRPITLRARNLPQPQSGQKNYECVFSIQGKVQRVPAVRFNSSCIQCQNTSYWYEGDELGDLPVDFSVVWDGDFFIDKPASMKALLYKCPAQRESCGLCLKAEPSFECGWCTAEKQCLLRQDCQAPEHNWLHPGRRNVRCSHPRITKIWPLTGPKEGGTRVTIVGENLGLHVREITHVRVAGVRCNPVPSEYISAERIVCDMEESLLPNPPGGAVELCIGDCVAEFRSQSVQIYNFVTPSFNKVHPDRGPVSGGTVITVSGRHLDAGSSVTVFLGQGECQFIRRSVREIVCQTPPSLAAPGPTNIKLYIDKAEIISPDTHYSYTEDPIVSSVEPGWSILDGSTPITVSGTNLLTIQEPRVRAKYGGVETTNVCHVVNDSVMTCLAPRITYTKRLPPESGLHPDEFGFILDHVTSLLALNGTPFTYYPNPTFDPLGNSGILEVKPGSPIILKGKNLIPPAPGNTRLNYTVLIGETPCLLTVSESQLLCDSPDLTGEQRVTIVVGGLEFSPGTLHIYSDSALTLPAIIGIGVGGGLLLVAIIAVLIAYKRKTRDADRTLKRLQLQMDNLESRVALECKEAFAELQTDIQELTNDMDGVKIPFLEYRTYAMRVMFPGIEEHPVLKELDSPASVEKALRLFSQLLHNKMFLLTFIHTLEAQRSFSMRDRGNVASLLMAALQGRMEYATMVLKQLLADLIEKNLENRNHPKLLLRRTESVAEKMLTNWFTFLLHRFLKECAGEPLFMLYCAIKQQMEKGPIDTITGEARYSLSEDKLIRQQIDYKQLTLMCVPPEGEVGTEIPVKVLNCDTVTQVKDKLLDAVYKGIPYSQRPQAEDMDLEWRQGRLTRIVLQDEDVTTKIESDWKRLNTLAHYQVVTDGSLVALVQKQVSAYNIANSFTFTRSLSRYESLLRTSSSPDSLRSRAPMITPDQETGTKLWHLVKNHEHADHREGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETVFSTAHRGSALPLAIKYMFDFLDEQAESRQTPVPDLNPLGPAVHLPLRFWVNVIKNPQFVFDIHKSSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPNYKTWVERYYRDIAKMPSISDQDMDAYLVEQSRLHANEFNTLSALSELYFYINKYKEEILTALDRDGYCRKHKLRHKLEQAISLMSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC29</accession>
    <name>W5NC29_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">AlkB homolog 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023852">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023853">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC29"/>
    <dbReference type="FunCoup" id="W5NC29">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018188"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018220.1">
        <property type="protein sequence ID" value="ENSLOCP00000018188.1"/>
        <property type="gene ID" value="ENSLOCG00000014771.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048626"/>
    <dbReference type="HOGENOM" id="CLU_059836_2_0_1"/>
    <dbReference type="InParanoid" id="W5NC29"/>
    <dbReference type="OMA" id="KSPKTKW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014771">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032862">
        <property type="entry name" value="ALKBH6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="100"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="239" mass="26854" checksum="A93478DF078AE742" modified="2014-04-16" version="1">IMEEPQSHVLKELESYTVKEAPPTVYYVPGFITETEEAYLLQQVYKAPKPKWTQLSGRRLQNWDPGGLPHPKGMLVEELPDWLKTYANKISALGAFSGKTANHVLVNEYKPGEGIMPHEDGPLYFPTVTTISLGSHTLLDFYRPISTAQEQEGVPQTEENRYLLSLLVEPRSLLVLRDDMYTRFLHGVRGVARDTVTESVANLRAVSAELGDSLSRGTRVSLTIRHVPRVLKFVVLLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCA5</accession>
    <name>W5NCA5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium and integrin-binding family member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Cilium</location>
            <location evidence="4">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628983.1">
        <property type="nucleotide sequence ID" value="XM_006628920.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCA5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018296.1">
        <property type="protein sequence ID" value="ENSLOCP00000018264.1"/>
        <property type="gene ID" value="ENSLOCG00000014834.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693080"/>
    <dbReference type="KEGG" id="loc:102693080"/>
    <dbReference type="CTD" id="10518"/>
    <dbReference type="GeneTree" id="ENSGT00940000157327"/>
    <dbReference type="HOGENOM" id="CLU_061288_6_0_1"/>
    <dbReference type="OrthoDB" id="114727at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014834">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000079">
        <property type="entry name" value="Calcium and integrin-binding family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051433">
        <property type="entry name" value="CIBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791:SF5">
        <property type="entry name" value="CALCIUM AND INTEGRIN-BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="187" mass="21750" checksum="E0087C09E22F51AF" modified="2014-04-16" version="1">MGNKQTIFTDEQLDAYQDCTFFTRKEILRLHGRYHELAPNLVPMDYTNDPDVKLPLQLIINMPELKENPFRNRIVESFSEDGQGNLSFNDFVDMFSVLSEMAPRELKAIYAFKIYDFNTDNFICKEDLEKTLNKLTREELTAEEVNLVCDKVIEEADLDGDGKLAFADFENMISRAPDFLSTFHIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCZ2</accession>
    <name>W5NCZ2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger and SCAN domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NCZ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018501"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018533.1">
        <property type="protein sequence ID" value="ENSLOCP00000018501.1"/>
        <property type="gene ID" value="ENSLOCG00000015031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684299"/>
    <dbReference type="KEGG" id="loc:102684299"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286936"/>
    <dbReference type="HOGENOM" id="CLU_002678_16_3_1"/>
    <dbReference type="InParanoid" id="W5NCZ2"/>
    <dbReference type="OMA" id="MKMEPEN"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015031">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002281">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001927">
        <property type="entry name" value="Zinc finger protein 1184"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="482" mass="54312" checksum="A5CB9842513C63AA" modified="2014-04-16" version="1">MANWLLILQSELHSFMEVLLKSIVCEVSEVFQNRMSDSEDKFQDKLRSISQILVRRAVFKISQCVEDSFGSEMAQLKKENESLKWRLQLLDKESGVGGDQGQTAEIKEEMDTKLEQSDSEALPDAGERALLEQQQSEEEWGSSLLQETELTAAEGKEKLSEQQTESRQSVEDLDSGHMMKMEPENSVASALPDAGERAPLEQQHSREEWGSSLMQETELTAAEGKERLSEQHTESRQSVEGQDSVHMMKTEPESEIHGPLVSEDFTEKINLNSHQDSSEHQSSKLKSDQCTHKGERVFICSQCGRSFSRSDHLKTHQLIHTGEKPFRCGQCGKTFTRTGSLKNHQLIHTGEKPFSCSLCGKSFLRADSLKTHQLIHTDEKPFKCGQCGKRFTVAGGLKNHKLIHTGEKPFTCSQCGKSFSQAGSLKTHQLIHTGEKPFSCSQCGKRFFQAGNLKTHQLVHTRERHRIRQYTEVTSRGSVCPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE97</accession>
    <name>W5NE97_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006071">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196806.1">
        <property type="nucleotide sequence ID" value="XM_015341320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE97"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018956"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018988.1">
        <property type="protein sequence ID" value="ENSLOCP00000018956.1"/>
        <property type="gene ID" value="ENSLOCG00000015406.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690851"/>
    <dbReference type="KEGG" id="loc:102690851"/>
    <dbReference type="CTD" id="572143"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222287"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_1_1"/>
    <dbReference type="InParanoid" id="W5NE97"/>
    <dbReference type="OMA" id="TFCNIFI"/>
    <dbReference type="OrthoDB" id="10063595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015406">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15329">
        <property type="entry name" value="7tmA_5-HT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000071">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001069">
        <property type="entry name" value="5HT_7_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF199">
        <property type="entry name" value="IP13425P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00652">
        <property type="entry name" value="5HT7RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="75"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="402" mass="44450" checksum="BBE003A47CC9C922" modified="2014-04-16" version="1">MFATSGVELALGHQDNVWEASNATTQLYLSNSLPASDPTANVSQSCGEQLLDFGTVEKIMIGVMLTIITAVTVMGNTLVVIAVCVVKKLRQPSNYLLVSLAVADLSVAVVVMPFVIVTDLTGGKWLFGEVFCNIFIGMDVMCCTASIMTLCVISVDRYLGITRPLTYPARQNGQLMAKMIAGVWLVSASITLPPFCGWAQNVNTAGVCLISQDFSYTIYSTAVAFYIPMAVMLIMYYKIFRAARKSAAKHRFASFTRRKDLETAANEGLKLQGLKPPGMAEECAALSRLLGRERKNISIFKREQKAATTLGVIVGVFSICWLPFFILSTARPFICGVHCSCIPIWLERTLLWLGYANSLMNPFIYAFFNRDLRSTYKDLLRCRYRNINRRLSAVGVHEALKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEI2</accession>
    <name>W5NEI2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Necdin-like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEI2"/>
    <dbReference type="FunCoup" id="W5NEI2">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019041"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019073.1">
        <property type="protein sequence ID" value="ENSLOCP00000019041.1"/>
        <property type="gene ID" value="ENSLOCG00000015466.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164454"/>
    <dbReference type="InParanoid" id="W5NEI2"/>
    <dbReference type="OMA" id="KITYSWG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015466">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1210:FF:000001">
        <property type="entry name" value="melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50838">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="55"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="30572" checksum="BB2F9292BE3B35FC" modified="2014-04-16" version="1">MSQFRKRVSARQSSQGTASRSQHTESKDEDDDVSFTQPTSSQVQRGLEHLTPTQVDQKVAEVVQYILIKDQKKVPIKRADIVRQVIKEYKNIYPEIIKRVNRTFEQVFGLKLVEIDSKNHIYILIKNLEHVEGDPVGTGTGTPKTGLLFVILAVIFMRGGVVKESVIWSTLKKLRVDQGERHKDFGDVKKLITEEFVRQKYLEYARIPHTEPLEFEFRWGLRADKEVSKLKLLTLVAQLHEQDPQTWATQHKEATQAEEKGPIATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFZ5</accession>
    <name>W5NFZ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heme-binding protein soul4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFZ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019554"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019586.1">
        <property type="protein sequence ID" value="ENSLOCP00000019554.1"/>
        <property type="gene ID" value="ENSLOCG00000015879.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUYX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165109"/>
    <dbReference type="HOGENOM" id="CLU_068699_2_1_1"/>
    <dbReference type="InParanoid" id="W5NFZ5"/>
    <dbReference type="OMA" id="PAGHWAC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015879">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000007">
        <property type="entry name" value="Heme-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF24">
        <property type="entry name" value="HEME-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="259" mass="29903" checksum="B58537DB32C51AEF" modified="2014-04-16" version="1">GCRLPVPPQLMARITFEDLAELEDEQLDNDVPEDNSEPMDEEEEERLLAHWQAVASTHQVSVPQEMSGPIQLMTRQSQVREQVPYVTVSRHEKLDETVYEERVYPAGKWACVTKGEDLYEQSISMAFMKLMRYICKENSTGRYLGMTVPVVNKIQILEDGTGFVREILTAYYLPAEFQDNPPDPTDPEIAIIQRGPLRVITRVFLGTTTEETITRQISLLWELLGSTDDFHRDMYMVAVYENPGVACRRNEIWFIRRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NG34</accession>
    <name>W5NG34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase S26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01021101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021102">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NG34"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019625.1">
        <property type="protein sequence ID" value="ENSLOCP00000019593.1"/>
        <property type="gene ID" value="ENSLOCG00000015913.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164174"/>
    <dbReference type="InParanoid" id="W5NG34"/>
    <dbReference type="OMA" id="HRAMANM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015913">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9967" checksum="235932F050F6E35D" modified="2014-04-16" version="1">RFLFPFQEMAQMGGFGRRYIKAFVSGFFIAVPVTVTVLDRLAYVARVEGASMQPSLNPEGGTTSDIVLLNRWSVRHYQVQRGDIVSVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIF8</accession>
    <name>W5NIF8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19">PR domain zinc finger protein 1</fullName>
            <ecNumber evidence="19">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection. Binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with PRMT5. Interacts with FBXO10. Interacts with FBXO11. Interacts with multiple nuclear sumoylation E3 ligases, including CBX4, PIAS1, PIAS2, PIAS3, PIAS4, PML and RNF4, but not RANBP2. Interacts with LDB1, SMARCD3 and SMARCC1. Interacts with EEIG1; following TNFSF11/RANKL stimulation in bone marrow-derived macrophages, the interaction promotes the binding of PRDM1/BLIMP1 to the gene promoter of IRF8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the class V-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01009149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626019.1">
        <property type="nucleotide sequence ID" value="XM_006625956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIF8"/>
    <dbReference type="FunCoup" id="W5NIF8">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020417"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020452.1">
        <property type="protein sequence ID" value="ENSLOCP00000020417.1"/>
        <property type="gene ID" value="ENSLOCG00000016531.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687063"/>
    <dbReference type="KEGG" id="loc:102687063"/>
    <dbReference type="CTD" id="323473"/>
    <dbReference type="eggNOG" id="KOG2461">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154798"/>
    <dbReference type="HOGENOM" id="CLU_007033_2_1_1"/>
    <dbReference type="InParanoid" id="W5NIF8"/>
    <dbReference type="OMA" id="LIMKMDM"/>
    <dbReference type="OrthoDB" id="9345291at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016531">
        <property type="expression patterns" value="Expressed in embryo and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060351">
        <property type="term" value="P:cartilage development involved in endochondral bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000578">
        <property type="term" value="P:embryonic axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048702">
        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007521">
        <property type="term" value="P:muscle cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060037">
        <property type="term" value="P:pharyngeal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051155">
        <property type="term" value="P:positive regulation of striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19187">
        <property type="entry name" value="PR-SET_PRDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000748">
        <property type="entry name" value="PR domain zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000019">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000132">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000211">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000262">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016608">
        <property type="entry name" value="PRDM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044413">
        <property type="entry name" value="PRDM1_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF68">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013212">
        <property type="entry name" value="PRDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="23">
        <location>
            <begin position="79"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="225"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="311"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="491"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="318"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="816" mass="91237" checksum="481C65B31A3143EC" modified="2014-04-16" version="1">MCGSTQSFPAATQHSSDILTAEESQETGEFTMKMDVEDADMTKWTELEFEEKCTYIVKDHPWESSTDSGSLTRAEASLPRNLLFKHAPNSKEVIGVTSKEYIPKGTRFGPLVGESYTTETVPKNANRKYFWRIYCNGEFHHFVDGFNEEKSNWMRYVNPAHSQQEQNLAACQNGMNIYFYTVKPIPANQELLVWYCPEFAERLHYPPSGDLMMLKLKQSLMEAKQNATETDSIQEKSSGKKEHSVSEILKGTRRDPVRPQSSPTKSLQDSPKKPSLEQPFFPRVVYPVRPHINEEYLKANTVYGLDRPSYVTHSPVQSSATPSPSARSSPEHSLQSSSPHSSPGLTLSPPTPSSQEHKEGFPFPNGLYSRGGLGHYPGYTPPSHLGPAFLHSYSSVSPHYSRYLLPHYPVGCNSITSLNGLGAGPVGSLFPRMYPLYSNLLSGGSLSQHMLSQSPLPSMLPSEGGRRLLFPSEPHRDLLIPAPNSAFSATGLASSLKDKPSSPSNGSPTAGTAASSSEHGMPTKPTSAMLASSRSEEEAINLTKIKRNITGYKTLPYPLKKQNGKIKYECNVCSKTFGQLSNLKVHLRVHSGERPFKCQTCNKGFTQLAHLQKHYLVHTGEKPHECQVCHKRFSSTSNLKTHLRLHSGEKPYQCKLCPAKFTQFVHLKLHKRLHTRDRPHKCLHCHHSYIHLCSLKVHLKGNCLASPNPRGRSPEELNHINEEIEKFDISDNADRLEEMDNVDVESAVEKQILNMLWREMDLKASYHKSMGNGLRSTGYGLYEPNNETSVIKLPHSSPLPLLSIKVKQESLEPIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK39</accession>
    <name>W5NK39_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E3 ubiquitin ligase TRAF3IP2</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Adapter protein CIKS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Connection to IKK and SAPK/JNK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">E3 ubiquitin-protein ligase CIKS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nuclear factor NF-kappa-B activator 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TRAF3-interacting protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with IKBKG/NF-kappa B essential modulator, with CHUK/IKK-alpha and with IKBKB/IKK-beta. Interacts with TRAF6; this interaction is direct. Interacts with IL17RA and IL17RC. Interacts with IL17RB.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01012028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK39"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020998"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021034.1">
        <property type="protein sequence ID" value="ENSLOCP00000020998.1"/>
        <property type="gene ID" value="ENSLOCG00000016988.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693738"/>
    <dbReference type="KEGG" id="loc:102693738"/>
    <dbReference type="CTD" id="606616"/>
    <dbReference type="eggNOG" id="ENOG502QTXH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164609"/>
    <dbReference type="HOGENOM" id="CLU_036721_1_0_1"/>
    <dbReference type="InParanoid" id="W5NK39"/>
    <dbReference type="OMA" id="TDMSTHE"/>
    <dbReference type="OrthoDB" id="6021171at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016988">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097400">
        <property type="term" value="P:interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038173">
        <property type="term" value="P:interleukin-17A-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11530:FF:000007">
        <property type="entry name" value="adapter protein CIKS isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053047">
        <property type="entry name" value="E3_ubiq_ligase_TRAF3IP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257">
        <property type="entry name" value="ADAPTER PROTEIN CIKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257:SF4">
        <property type="entry name" value="ADAPTER PROTEIN CIKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SEFIR" evidence="14">
        <location>
            <begin position="369"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="70"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="539" mass="60099" checksum="6755910F31CF68D1" modified="2014-04-16" version="1">MESFIDFRPLRSIPVETDESMVFLDVLAKCKDQPCGTAGVCSGQPEVTVNEVALCRSGVQDSLSGRNYFQEKKHQTGASEEDTGQSPGKSPFPAGSWADSSPEMRRFEITQAPRGPAKPTNTCLQQPGSSEVGPCPAVKRRQLPTEKDFESEVGSDSRESARPSLLSRQLDLPSEDTGYGSQPRKDMDVQLQDPPLSLRSDLGCPVGPGVVINRGEGSGLWPTAHNNPQQHHHYPAPQRFAAVDCGHACWSHSPSYQRQDPRVCAQQQLTGCTHGRCVPEPQQEVRLWPGPEGWNQPHFRFGVRDSHSSAYGLHRGLHSTPGCAAPSVGVMSQIDIVPSPPLPPPYRQPAVSGTEEPGMLKTINLPDECRNLFVTYSVDAAREILTFVEFLTKQGFRPAIDIFDNPIRRMDINKWMDSYLKDKSVLIIVVVSPKYKEDIEGTGLDEHGLHTKYIHTMMQNEFIQQGSLNFRFIPVLFPNATKRHVPGWLQNTRIYRWPSETEDLLLRLLREEKYISPPLGKELTLHIRPVGPNQLIKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKA3</accession>
    <name>W5NKA3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <subcellularLocation>
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            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1108"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1146"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1162"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1215" mass="133141" checksum="7A07C0EF3073C7DB" modified="2014-04-16" version="1">MNNGSCEDLANDYYCRCLRGFTGKNCEDIIDYCRLLSVNCLNEGLCLNIIGGYSCLCAPGWTGEFCEYVENACLIYPDRCTKGATCIDVSLPKTPPKYKCICQHGYTGNDCLNDLHSNSSFQQTCANGGRCFLKDAESYTCVCPSGWTGHNCLENIDECEEHWCQNGATCEDAINGYRCVCPRGFTGPFCESSIDYCLGSQCSNLSTCVVQQYSYTCRCMLGYEGQFCEMEINECNSSPCKNGARCVDLVGDFLCHCAVGFEGRTCSENVDDCWSRPCLNGGSCIDLINKYQCLCPFGFEGLNCEINFNECDYGFCTNNSTCTDLVGDYECTCPPGFTDKNCSTDINGCESEADPCRNGGTCYNLIGEYRCVCAPGFEGDECSVEVNEYISNPCDPGRSLRCEDLVNDFKWICQAGHIGQLCEIPISHCVKGLCHYGSMCEDVPGGFLCHCLPGFTGDFCEVNIDNCQERPCGALSICRDALDSYECFCAPGFIGNNCEIEVDECRSDPCQNGGTCFDELDAFSCLCPAGIEGSSCEINTNECQSLPCLHNATCTDLINGYECSCLPGFTGAHCETDIDECASSPCKNGATCIDQPDLKRFRCQCVTGFTGPRCEINVNECSSNPCLNGYCYDVLVVGGFYCLCNPGFAGVRCEQDIDDCVNHVCENNSTCLDLHLTYQCLCLPGWEGEFCQQEIDECASYPCKNNASCTDLFNSYSCTCALGWKGEDCNEDVNECESSPCLNGATCIESALPGIFKCICPPFFTGGLCKQPYDPCDTHYNPCMHNSTCLTQINGTAICICHEGMGLQSYIYWTSNLRTFKESCTTIRFEGIHCEIDTNECSSGPCQNQGHCLDGVSSYSCECKPGFSGSHCEEDINECASIPCKNKGTCHDLINRFRCDCTPGYFGSLCELDVNECEASPCLHEGSCINMPGGFQCVCLPGFSGKWCEVDVNECASDPCLNSGRCIDDVNRYQCLCARGFMGSNCETNIDECASNPCLHGRMQKTEKASLSFSCTDEINKYVCHCEPGWTSERCEININECDSNPCFNGASCVDLVNKYACFCLDGYYGKLCEIDMDVCQDSPQTFSLCFNGGTCLDGPAANFTCRCPSGFLGDFCEVEINECFSAPCLHGAICQDLVDGYLCHCRPGWTGRHCENDINECQSGPCDQGMCIQKQPGHGYTCFCRPGFVVRNGKNCEHNYNDCLIQSCPDEYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKD8</accession>
    <name>W5NKD8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-acetylglucosamine-6-sulfatase</fullName>
            <ecNumber evidence="6">3.1.6.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glucosamine-6-sulfatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.</text>
            <dbReference type="EC" id="3.1.6.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.14" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01035203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035204">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035206">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKD8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021097"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021133.1">
        <property type="protein sequence ID" value="ENSLOCP00000021097.1"/>
        <property type="gene ID" value="ENSLOCG00000017070.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3731">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158420"/>
    <dbReference type="InParanoid" id="W5NKD8"/>
    <dbReference type="OMA" id="GHNENWL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017070">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008449">
        <property type="term" value="F:N-acetylglucosamine-6-sulfatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16147">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000224">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012251">
        <property type="entry name" value="GlcNAc_6-SO4ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF5">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036666">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="38"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="7">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR036666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036666-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="546" mass="61514" checksum="1FFE7B2AB0F3E091" modified="2014-04-16" version="1">KKKMISIRLWLTALLNYLLVSAGWFPTYSRVEATFHRPNIVLILADDLDVAIGGMIPLKKTKKLIGEAGISFTNAYVASPLCCPSRASILTGKYPHNHMVVNNTLEGNCSSPAWQKRHEPNTFPALLSRHAQYQTFFAGKYLNEYGNSEAGGIECLPMAQKKWIDMEKNSKYYNYTLSVNGKARKHGENYSKDYLTDVLANASLNFLEYKSSSRPFFLMISTPAPHSPWTAAPQYEKSFADVRAPREANFDVHGKGKHWLIRQARTPMSNSSVQFLDDVYRRRWQTLLSVDDLVEKLVQHLDTSGELQNTYIFFTSDNGYHTGQFSLPVDKRQLYEFDIKVPLLVRGPGIKPNQTCQMLVANIDLGPTIMDIAGHSVNPTQMDGMSFLPLMSGQGNNVTWRTDVLLEYNGEGGDDGDPSCPVLGPGVSRSKQCFPDCVCEDASNNTYTCVRTLARGANLQYCEFEDSELFVEVYNLTADPFQLINIAKTIDQEVLEKMNRRTIMLQTCSGLSCRTPGVFEPRYRSDARFVLNSNHPRARELLKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKG3</accession>
    <name>W5NKG3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PR domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035280">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035281">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKG3"/>
    <dbReference type="FunCoup" id="W5NKG3">
        <property type="interactions" value="1206"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021158.1">
        <property type="protein sequence ID" value="ENSLOCP00000021122.1"/>
        <property type="gene ID" value="ENSLOCG00000017092.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2461">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156443"/>
    <dbReference type="HOGENOM" id="CLU_495729_0_0_1"/>
    <dbReference type="InParanoid" id="W5NKG3"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017092">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000840">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
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        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
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        <location>
            <begin position="164"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="550" mass="58352" checksum="4758751EF3A49728" modified="2014-04-16" version="1">MNDMNLSPVGMDQLSVPSVSGAIPVSGGHLGLPTSPPHSSIRNGFLSLYVPAQHTPHVGVTQVSKGAVMPWFCSGNNRIDELLTADVAGPVRFVTPPVLSCLIAVGILSYLADRPPPQYIHPSSLNMDAGAPLSVPSNPSSLDPYQPGGAVGLEPGMVALDSRQQVGGHGAGGLHQGGAHEVPLDAGLSLDPPRVSSPISPDGMGEEPARSHEGMAVDGGAGVMPLHAGGGLELPVVLEPELHPAGLSSGPVSVVLSHPIAPLEPAGMGLEPVNAAAAAAGTEVPLGPENLVLVPSVLQLEDSNSNKENLAAAFTICECRPRGGGGFSGRLAGPAARGGSSLIPPPPPSSFPRSTGEEQNLVAYPHSGKLYFCSSRDIPPDHELLYYYSRDYARQIGVPEVPDAQVCHCRKECSSYCVWERTENDKRSSTLPPGGRGVQLQTEHRFPKIVGDPAVLQRVGPPLAPAGQKNYRCSVCEKSFTQKAHLASHMIIHTGARDLKCEHCDRLFMRRQDLKQHALTHTQERQKKCPRCDKLFWRTNHLKKHLNSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKQ6</accession>
    <name>W5NKQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkey-16j16.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015212739.1">
        <property type="nucleotide sequence ID" value="XM_015357253.2"/>
    </dbReference>
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        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000021251.1">
        <property type="protein sequence ID" value="ENSLOCP00000021215.1"/>
        <property type="gene ID" value="ENSLOCG00000017163.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078600"/>
    <dbReference type="eggNOG" id="ENOG502QQDQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001612"/>
    <dbReference type="HOGENOM" id="CLU_087169_0_0_1"/>
    <dbReference type="InParanoid" id="W5NKQ6"/>
    <dbReference type="OMA" id="PQRHSDH"/>
    <dbReference type="OrthoDB" id="6435011at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:peripheral nervous system development"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR41142">
        <property type="entry name" value="SI:DKEY-16J16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41142:SF1">
        <property type="entry name" value="SI:DKEY-16J16.4"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="271" mass="30697" checksum="88BF5B833D4E19E5" modified="2014-04-16" version="1">MLKTLTKKLRRQSLNEIHPFQFKISYHGSNEGGESEDSEGENQELVQIDRERRRNCGLTPLATSQQHNQEALSPARLRRLRLLLDPGLDRHSSEEELERISRDFLQPPGEGRKWSLRQAGGPGWYGEPSTSSSDEEVKDLCELAERDPCLATSSSPVLFSASPPHRLKPLVRGQARPIILAHLDQPAPYRRHRHGSRGEQRRPSLDLEKMQQKMFLKKNCGGKTRTVKIWGSNSSRSLPRYTYDPSTFAFRSLSTAPPCSPRTPPEEPPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLG4</accession>
    <name>W5NLG4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218133.1">
        <property type="nucleotide sequence ID" value="XM_015362647.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015218140.1">
        <property type="nucleotide sequence ID" value="XM_015362654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLG4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021473"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021510.1">
        <property type="protein sequence ID" value="ENSLOCP00000021473.1"/>
        <property type="gene ID" value="ENSLOCG00000017368.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696792"/>
    <dbReference type="eggNOG" id="KOG4220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160084"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_2_1"/>
    <dbReference type="InParanoid" id="W5NLG4"/>
    <dbReference type="OMA" id="TWACDLW"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017368">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045987">
        <property type="term" value="P:positive regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046541">
        <property type="term" value="P:saliva secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000047">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000103">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001183">
        <property type="entry name" value="Musac_Ach_M3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF183">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00540">
        <property type="entry name" value="MUSCRINICM3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="593" mass="67571" checksum="9D49BABF5B28B9C7" modified="2014-04-16" version="1">MNLRYNSTEHTFLTSNSPTMRGFQDAEILQDSRWHEHINSVENDSFNSGNPNLTSLYNATQTPPTTDPLGGHTVWQVILIVFFTGLLSLVTIIGNILVLVAFKVNKQLKTVNNYFLLSLAFADLIIGIISMNLYTTYIIMDQWALGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWSISFVLWAPAILFWQYFVGERTVPSDKCYIQFLSEPIITFCTAIAAFYLPVTIMTVLYWRIYKETEKRTKELAGLQASGREAETTHFVHQTGSSRSCGSYELTQPSLKRSARRKQGRFNFWPIMMSWKHSSEGEQDHSSSDSWNNNDAAASLDQSGSSDEEEIPETRAIYSIVLNLPGIKAAVNSQLTSPDDLEVSEEDPLGVDRKEGLSRKEKEEKADNNYHQHFSKMPVQSMPSIQTPKTEETVSSATKSSSMPISFKDAAMAKRFASKARTQITKRKRMSLVKEKKAAQTLSAILFAFIITWTPYNIMVLVNTFCDNCIPETLWALGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCQWDKRKRRKQQFQQRQSVIFHKGIPRDPS</sequence>
</entry>
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    <accession>W5NLI9</accession>
    <name>W5NLI9_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Family with sequence similarity 43 member A</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="346" mass="38857" checksum="76DD290B754CAFF9" modified="2014-04-16" version="1">MLPWKKNKFDLIEEDKQSKQKGYAVSLNYSALTSFAKSCPESALNRVGSMFKSKRKKVKITSEDPTYTVLYLGNATTIQSKGDGCTDVAVGKIWTKSEMGKNGTKMKLTISSQGIRMVHVDDKARRPGHLYLLHRITYCVADPRLPKIFAWIYRHEMKHKAVMLRCHAVLVSKPEKAKAMALLLYQTSATALAEFKRLKRRDDARHQQQQLIGEQTIPLVPLRKLLNGQCYYKPPVERSRSAPKLGSITEDLLGEEEEEKAMHFECEDILDTDDDCLGSGKQELSQIINDLGEMCIGNDVQTLKADLRVTRLLSGESTGSESSIESNQEPNSISNGFEEVKAQEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMM9</accession>
    <name>W5NMM9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Oxoglutarate (alpha-ketoglutarate) receptor 1a, tandem duplicate 3</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000021926.1">
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        <property type="component" value="Linkage group LG17"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF15">
        <property type="entry name" value="2-OXOGLUTARATE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="P2YPURNOCPTR"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="327" mass="37839" checksum="43EAB69E0FBC9285" modified="2014-04-16" version="1">QKMGQINSEEVYITPPNCTNIDTLMKRYYLPVMYSIIFIVGLVGNIISIMIYVFKMRPWKSSSIIMLNLAISDLLYVLSLPSFIYYYINQDSWTMGEFMCKLSRFVFHFNLYGSILFLTCLSIFRFIVVVHPLRVAQVQKRKWGILACVIVWVITAAELTPTLLMVNLIDVNHTMQCLDFASNHRETVWWYGLFLTILGYLIPLVIVCACYCRIVQALGKGPHVQSACRVKARKLIVVILLVFILCYLPFHILRILRVDTRRPGVDCVTMRRVHAAYIITRPITGLNTFFNLGLYTLAGDQFQQAFLSLFKSQQRFFMHAINVEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMU1</accession>
    <name>W5NMU1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynein axonemal light chain 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMU1"/>
    <dbReference type="FunCoup" id="W5NMU1">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021950"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021989.1">
        <property type="protein sequence ID" value="ENSLOCP00000021950.1"/>
        <property type="gene ID" value="ENSLOCG00000017847.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166105"/>
    <dbReference type="HOGENOM" id="CLU_070944_5_0_1"/>
    <dbReference type="InParanoid" id="W5NMU1"/>
    <dbReference type="OMA" id="SKVTHES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017847">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21453">
        <property type="entry name" value="DLC-like_DNAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000002">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="56" mass="6330" checksum="E5722F000CE2DC5F" modified="2014-04-16" version="1">RQSAAKMIKETMDKKFGSSWHVVIGEGFGFEITHEVKNLLYMFFGGSLAVCVWKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNI5</accession>
    <name>W5NNI5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Somatostatin receptor 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNI5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022194"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022235.1">
        <property type="protein sequence ID" value="ENSLOCP00000022194.1"/>
        <property type="gene ID" value="ENSLOCG00000018093.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165523"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_1_1"/>
    <dbReference type="InParanoid" id="W5NNI5"/>
    <dbReference type="OMA" id="QEDFQTC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018093">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004994">
        <property type="term" value="F:somatostatin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071385">
        <property type="term" value="P:cellular response to glucocorticoid stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15974">
        <property type="entry name" value="7tmA_SSTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000039">
        <property type="entry name" value="somatostatin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000586">
        <property type="entry name" value="Somatstn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001184">
        <property type="entry name" value="Somatstn_rcpt_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF20">
        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00246">
        <property type="entry name" value="SOMATOSTATNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00591">
        <property type="entry name" value="SOMATOSTTN5R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="62"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="369" mass="41519" checksum="4E13B73C7EFD6738" modified="2014-04-16" version="1">QLCARMDLAGNSAWAVSPEAFNQTLLTNISRNGTIQSVPLDGISTVLIPVIYLTVFTIGLTGNTLAIYVVLRYTKMKTVTNIYILNLAVADELFMLGLPFLTTQNVLSYWPFGSFLCRLVMTVDAINQFTSIFCLTVMSIDRYLAVVHPIKSTKWRRPRVAKVINASVWILSFLVVLPVIIFSDVQDGLNTCNINWPDPNAVWSTTFIIYTSVLGFFGPLLVICLCYLLIVVKVKSSGVRAGFTKRRKSERKVTRMVVIIVVVFVCCWLPFYILNMVNLVFIVPENNVMAGIYFFVVILSYANSCANPLLYGFLSDNFKQSFRKVLCIYKGNGVEDGDPSLPRTEKTTFHDSFLSHRNNDFNGHMQTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NP88</accession>
    <name>W5NP88_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Claudin 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213430.1">
        <property type="nucleotide sequence ID" value="XM_015357944.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015213431.1">
        <property type="nucleotide sequence ID" value="XM_015357945.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NP88"/>
    <dbReference type="FunCoup" id="W5NP88">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022447"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022488.1">
        <property type="protein sequence ID" value="ENSLOCP00000022447.1"/>
        <property type="gene ID" value="ENSLOCG00000018346.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686772"/>
    <dbReference type="KEGG" id="loc:102686772"/>
    <dbReference type="CTD" id="9069"/>
    <dbReference type="eggNOG" id="ENOG502QR4G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022250"/>
    <dbReference type="HOGENOM" id="CLU_063070_0_0_1"/>
    <dbReference type="InParanoid" id="W5NP88"/>
    <dbReference type="OMA" id="FYNTHLN"/>
    <dbReference type="OrthoDB" id="3031595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018346">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013287">
        <property type="entry name" value="Claudin12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16703">
        <property type="entry name" value="CLAUDIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16703:SF3">
        <property type="entry name" value="CLAUDIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01872">
        <property type="entry name" value="CLAUDIN12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="327" mass="35704" checksum="66A4111A7F4B4CCF" modified="2014-04-16" version="1">MTCRDIHATTVFAFVIALFSVGGLVIATLIPQWRVTALTTFNKNAKNVTVYDGLWAKCVRLEGSTNCLIFDSEWYSRVDQLDLRVLQFALPISIFFSSLSLFLGLTGMCKTACCSDVPDVPLTKCLVNSAGCHLVAGIFYFLSGGIGMAPSVWFIFYTSNLNKKFDHIFSTDFAAYVAIGSSGGLLLAACLLFLWYCLCKTLPSPFWLPLYSQPAAVHAYPPHSPPSNSYSSPVYPPQACVPQSYGSQVSNPQAYVSQTSVPQGMGPHTYMSQISAPDGYGSEVCASQAYAPQSYTSQSYGSPDYRYSTRSRLSTIEIDIPLHGQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NPB0</accession>
    <name>W5NPB0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Olfactory receptor, family 90, subfamily H, member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NPB0"/>
    <dbReference type="FunCoup" id="W5NPB0">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000022510.1">
        <property type="protein sequence ID" value="ENSLOCP00000022469.1"/>
        <property type="gene ID" value="ENSLOCG00000018368.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXHR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161337"/>
    <dbReference type="HOGENOM" id="CLU_077059_0_0_1"/>
    <dbReference type="InParanoid" id="W5NPB0"/>
    <dbReference type="OMA" id="VWVLLFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF998">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="34"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="310" mass="35658" checksum="B71AB874C681C1B5" modified="2014-04-16" version="1">MNSTTAAFLNQDVFYFAFLKNFTVVLLGVVINYMNTMLVFTFFKNQVFSDNPRYILYIHMVINDILLLTLTVSLHVLSYIFQALNVSLCCFILLIAANATMNTPLNLAGMAIERYIAICHPLRHSQICTVRRTYILIAFIWCAGAIPRLTDLFILFATKPLSLFSSNILCYSYDVFQLPYKEEKNYATHAVYLFSVWLILIITYFRILFAAKAAASDAASAKKARNTILLHGAQLLLCMLSYITPVIDTIMLSDFPQYRTHILFLTYLVSHIVPRILSPLIYGIRDEQLKKHMKRYILCKQIVQIRPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVR4</accession>
    <name>W5LVR4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin specific peptidase like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">USPL1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVR4"/>
    <dbReference type="FunCoup" id="W5LVR4">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000221"/>
    <dbReference type="MEROPS" id="C98.001"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000221.1">
        <property type="protein sequence ID" value="ENSLOCP00000000221.1"/>
        <property type="gene ID" value="ENSLOCG00000000198.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRFM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002316"/>
    <dbReference type="InParanoid" id="W5LVR4"/>
    <dbReference type="OMA" id="NANALCW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000198">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030576">
        <property type="term" value="P:Cajal body organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029388">
        <property type="entry name" value="DUF4650"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028890">
        <property type="entry name" value="Peptidase_C98"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033505">
        <property type="entry name" value="USPL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15294">
        <property type="entry name" value="RETINOVIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15294:SF3">
        <property type="entry name" value="SUMO-SPECIFIC ISOPEPTIDASE USPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15509">
        <property type="entry name" value="DUF4650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15499">
        <property type="entry name" value="Peptidase_C98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="235"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="870"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="718"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1089" mass="118240" checksum="A4C3C621BBFD027D" modified="2014-04-16" version="1">MVMLHKWPRIPTDKRSCSDFPVMSSESTAIGESAPLVAGYLGKSQERLHLEKNCPVCAAKGQTQALRAFHISFQESIILCGNPQCIFPLGSKPLADILLHGTSEKPHIANSYARKRTLSSSGETQAEPSSKSPKAAEEIDPAQNTSGTRVVCKPCSVVLTYSKVCESKLQTEGQNVHYVDTRSPPKELDFSCPDPCSPGKDTKQPSPAKNKPCEVTSEGVQEGSSEELVPAPPFLFWKNQHSLCWLDSLMVVLVHSSAVRENLNTLQSDSSPLRRLCTEYDKAQALLQEAQRTSSEGEVGKIPSGVLLQAESLLNEIRMSVFNLLQPKLRCKLGSQESPVFALPLLLQLDQVTEELFKSSFHWEFACFACGHSSRSRRENTLTTFTGIIPEWNPLNAAHASPCPRCDHKHQMRKMVLERVPPVFMLHFVEGLPHNDMNAYAFDFQEMQYAVCAVIQYDQLVKHFVTWLHNEDGTWLECDDLKYPLCIHHKHLEVPSKEIHIVFWDACRKSTVTGSGLHLKEECVKHVPPEEGTAAEADALDTSLPLLEVDSSLSDIVAQEESDDLSAHAGLNATLTNSDMRDAFEGLSHNDIVTLTLVEVKVDAEGRPLDSHRVPDSTDCSDQTAAPEENRPGGEDFGLLNNSSGSPPPRVNQSSETPASLLCPAELCGPQTRSSEKAPVSVQSSQQTPREKRVTVDSKNREEFGTAAKVPPASDVRTALSPDTTSPPAATTTALPATAAASAVNGGKSGGSGDKWSVHFLNRHPVLRSSSTHFKAVPVAPSRPSQPFPTREQQSPRPLLALGDDGNFPGKSAEMFGGFRSKGVATSKGSLPSRETGLKMQDVSEEALLKPPCPPSPCLPLSKANVISSPSSDSAGFPVNATKPDPRKAVSTTSALKGHDKTDKLRYKLLKKLKAKKQKLASLNSLLKLNGFGDTSVLPESHKSTVHLDAKKILQRLESTDTESMYTVSSASSLCASPSYDEFFADLLSPSTTDSSVSPDSTDCLERLANGKQVRGLLDGSTTKQNCYQGSIPLQTQGPNLAVSESPFAVTQNGLCSTGEDIFEELMSNSTLQSCEAESTDLSTFDLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW14</accession>
    <name>W5LW14_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW14"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000321"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000321.1">
        <property type="protein sequence ID" value="ENSLOCP00000000321.1"/>
        <property type="gene ID" value="ENSLOCG00000000293.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167909"/>
    <dbReference type="HOGENOM" id="CLU_001324_5_7_1"/>
    <dbReference type="InParanoid" id="W5LW14"/>
    <dbReference type="OMA" id="DARNDIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000293">
        <property type="expression patterns" value="Expressed in intestine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786:SF74">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45786">
        <property type="entry name" value="DNA BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="1">
        <location>
            <begin position="273"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="390" mass="44814" checksum="B19352D7AC29297D" modified="2014-04-16" version="1">VVCNHCSVKTWKDEPPGLCCNNGNVSLLSIEQPPEPLLSLMDGDTEKSRHFLSNIRKYNSCFQMTSFGASKELNEPGLKIQGHVYHRIGLLLPVEEELPKFVQIYFMGDEQESIDPRCHLMPGVHLEVIQSLQNMLHEHNNLVRSFKSVLQDPALGNHKVIIRADKTQAGEHKSRFNAPNTDEVATLLVDQDAEKYDIILHQRNDTHPRIPETHRSYDALQYPIIFWKGQDGYFINILSLSSGKKVTASDYCNYGEIKQFQSYFEVQRTIPPVHQYIIDMYAKTESEHLLFICQNQSTLAETYQGLRDAITSDGDINIGKRVILASTFTGGPRYMHEKTQDDYGRPDLFITFTCNHTWQEVQRELFPGQKAIHRHELITRVFQRKVVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWA6</accession>
    <name>W5LWA6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWA6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000413"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000413.1">
        <property type="protein sequence ID" value="ENSLOCP00000000413.1"/>
        <property type="gene ID" value="ENSLOCG00000000364.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="HOGENOM" id="CLU_006842_0_4_1"/>
    <dbReference type="InParanoid" id="W5LWA6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000364">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF127">
        <property type="entry name" value="SERINE PROTEASE 27-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="11"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="246" mass="26846" checksum="07AD3ECE5C98F221" modified="2014-04-16" version="1">VSSSPLSRSSIVGGEDAGDGLWPWQVFLLVKKTNGSLSQCGGSLISKHWVLTAAHCFKGYIDIKSSHVYLGAYKLDLPSSHQVNRNIADVVIHSKYTITRKGYDIALVKLDRPVPLSRFIQTVPLAEPSDLFTSRSDCWVTGWGRVQQNICLSLSNHSAGQYSLSPITVQSVWNQSQSELESSPLCLSVSLSLQGDSGGALVCKKGNRWVQAGIVSYGKGCELPIPPKIFTRVTSYRPWIKTHSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWD7</accession>
    <name>W5LWD7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWD7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000444"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000444.1">
        <property type="protein sequence ID" value="ENSLOCP00000000444.1"/>
        <property type="gene ID" value="ENSLOCG00000000409.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LWD7"/>
    <dbReference type="OMA" id="DGAEYFC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000409">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865210" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="133" mass="14500" checksum="8D176D4B93A5D560" modified="2014-04-16" version="1">ATMIFITIFLWTLLISAQESSGQVTVTQTPAVVSVLPGQTVTVSCKASPAVHNDNQLAWYQQKAGEAPKPLILYATDRQSGIPKRFSGSGSGTDFTLTITAVQAEDGAEYFCQSLHNINSYWPRTQCYTPLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX59</accession>
    <name>W5LX59_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myotonic dystrophy protein kinase coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LX59"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000716"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000719.1">
        <property type="protein sequence ID" value="ENSLOCP00000000716.1"/>
        <property type="gene ID" value="ENSLOCG00000000648.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0612">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2176765_0_0_1"/>
    <dbReference type="InParanoid" id="W5LX59"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000648">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014930">
        <property type="entry name" value="Myotonic_dystrophy_kinase_coil"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08826">
        <property type="entry name" value="DMPK_coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotonic dystrophy protein kinase coiled coil" evidence="8">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="31"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="110" mass="12345" checksum="4D2E567DA8DD7D6F" modified="2014-04-16" version="1">MEGLKASDRATARQDSQWKARRLLKTEASARLELQSALEAELRAKQSLQQELSTLKAASLSTESKLQEVEEQNTELRAEVERLKKEIKSLPVSDLRGRDSIVTLMPLSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXK3</accession>
    <name>W5LXK3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ERI1 exoribonuclease 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exonuclease domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ERI2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXK3"/>
    <dbReference type="FunCoup" id="W5LXK3">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000860"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000864.1">
        <property type="protein sequence ID" value="ENSLOCP00000000860.1"/>
        <property type="gene ID" value="ENSLOCG00000000776.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0542">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="HOGENOM" id="CLU_025844_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXK3"/>
    <dbReference type="OMA" id="TSPLCGC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000776">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000062">
        <property type="entry name" value="ERI1 exoribonuclease 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="565"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="486"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="632"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="658" mass="72382" checksum="70C924A463C8AB0C" modified="2014-04-16" version="1">MATKLLAKKLGLIRKRSQPSSNGAQRRPGAKQFFSYLVVIDFESTCWRDKNHCSPEIIEFPAVLLNTSNGRIDSEFHTYVQPQEHPTLSEFCTELTGIRQSQVEAGVPLKICLSQFNKWLQTLQQEENIVFVNDLVRPSDSVANPCTFVTWSDWDLGVCLQYECKRKQLHRPDALNSWIDLRAMYKSFYNRKPKGLTGALQDLGIKFSGREHSGLDDARNTAHLAWRMITDGCVMKITKCLERVNSNVWVSQVKPDPGNRCANSEAARDNHHGNCVARGENNTEDRQTKSVLEQVKLDHVQRKQSSKNKEVLGLRSNNENHLCQSLVYPKTLFHGLMPCVAQENPPVPKSISSGTLVTINSPTVLSTNQVLVSTMVDCVTDVSDLASYIATDSETVLGEWEGNMVIPAVDEGRSYDSVVLEDPEKTDSVRSPNWTFSQKFQQQEDGVLTNSKTSNEVLFKRPNPAVRSTANVSVCPSNRTTCRVPNFSSPAIRPAKGAKQTSLSSMSFFTKPQKGSSSLTVYKNPVCPSNSSLSSNRSDAGSAPPPVLASLVSGSSSGKVTPPLCGCGRRSKRLRVSNGGPNHGRAFYRCPAGPTVSGRNGNCGFFKWEAALGKGKPAAAAWSRSWDCRDRSTSSEQSFAGGGSDSAQHRSLRPSLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5LY12</accession>
    <name>W5LY12_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01005585">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LY12"/>
    <dbReference type="FunCoup" id="W5LY12">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001019"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001023.1">
        <property type="protein sequence ID" value="ENSLOCP00000001019.1"/>
        <property type="gene ID" value="ENSLOCG00000000911.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685629"/>
    <dbReference type="KEGG" id="loc:102685629"/>
    <dbReference type="eggNOG" id="KOG4275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012719"/>
    <dbReference type="HOGENOM" id="CLU_041431_1_0_1"/>
    <dbReference type="InParanoid" id="W5LY12"/>
    <dbReference type="OMA" id="GSEQSCK"/>
    <dbReference type="OrthoDB" id="6339724at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000911">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049320">
        <property type="entry name" value="CARP1_2_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21272">
        <property type="entry name" value="FYVE_CARP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="21">
        <location>
            <begin position="39"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="297"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="133"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="344" mass="38049" checksum="FF1D704E4B17E26D" modified="2014-04-16" version="1">MWASCCNWMCLDTTTVEETSSTRRQAYTNSGYSSYPSPPAPEQTCKACGGRFDTSSRKHVCTDCKKSFCSRCSEQPEFGLRVCHTCRRFHGILSERAELMKLKVKDLRDYLHLHEVSTQMCREKEELVELVMGRQTPSSSSSSHGDAQSHTPQPGPTPGPASPSTSQGPAPGCLPRGDQQIPVPSCPGETPASETEPAPQEEQESQSSDAEETPAPGRRASLSDLTNAEDIEALSVRQLKEILARNFVDYKGCCEKWELMERVTRLYHDQQELQNLVSNNGNSTDPSGPPGMEENLCKICMDSPIDCVLLECGHMVTCTKCGKLMSECPICRQYVVRAVHVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ34</accession>
    <name>W5LZ34_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cilia- and flagella-associated protein HOATZ</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HOATZ family.</text>
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    <dbReference type="EMBL" id="AHAT01024005">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="W5LZ34">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000001396.1">
        <property type="protein sequence ID" value="ENSLOCP00000001391.1"/>
        <property type="gene ID" value="ENSLOCG00000001229.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S8UY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166624"/>
    <dbReference type="HOGENOM" id="CLU_122904_1_0_1"/>
    <dbReference type="InParanoid" id="W5LZ34"/>
    <dbReference type="OMA" id="TVCSERQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001229">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040681">
        <property type="entry name" value="HOATZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47231:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN HOATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47231">
        <property type="entry name" value="UPF0722 PROTEIN C11ORF88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17664">
        <property type="entry name" value="HOATZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13421" checksum="2A8E1F5DD22BBF19" modified="2014-04-16" version="1">EQYFTVFAGSSVEDVSYAKLFWSSASLNPPLESRLVSGDTRQRLRTAGGPQARESAHAGPGPGGCEEFLHEAYLQQKAEEKQRYLEKAKKREELLALLQKQRADRIRKEKISQPYRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ90</accession>
    <name>W5LZ90_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin, light chain 9a, regulatory</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01021233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ90"/>
    <dbReference type="FunCoup" id="W5LZ90">
        <property type="interactions" value="1098"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001447"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001452.1">
        <property type="protein sequence ID" value="ENSLOCP00000001447.1"/>
        <property type="gene ID" value="ENSLOCG00000001277.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155458"/>
    <dbReference type="HOGENOM" id="CLU_061288_9_3_1"/>
    <dbReference type="InParanoid" id="W5LZ90"/>
    <dbReference type="OMA" id="GVNFTMF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001277">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032036">
        <property type="term" value="F:myosin heavy chain binding"/>
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    <dbReference type="GO" id="GO:0030239">
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        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="174" mass="19947" checksum="4C5ACE36CCC9C3A0" modified="2014-04-16" version="1">TMSSKRAKGKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYTEFTRILKHGAKDKDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZD9</accession>
    <name>W5LZD9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01021229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021230">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZD9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001496"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001501.1">
        <property type="protein sequence ID" value="ENSLOCP00000001496.1"/>
        <property type="gene ID" value="ENSLOCG00000001295.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160046"/>
    <dbReference type="HOGENOM" id="CLU_002546_0_1_1"/>
    <dbReference type="InParanoid" id="W5LZD9"/>
    <dbReference type="OMA" id="GSRWWFS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001295">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF62">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE LPIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="7">
        <location>
            <begin position="734"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="642"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="945" mass="104420" checksum="584763502844EF21" modified="2014-04-16" version="1">MNYVGQLAETVFVTVKELYRGLNPATLTGGIDVLVVRQPDGSLQCSPFHVRFGKLGVLRSREKVVDIEINGEPVELHMKLGDNGEAFFVEESEDLQDRVPAHLCTSPIPLESAEDCEAPGTPLSGASRRKKRRRKRTRSDAPWREEGSSSSDERDVPESEGSGPELPAHLAASSSVYYSFSEDPALETGNTLSRDVHPYSDGEWSLSESVFQSRPSSPKSDSELLVRPQESMGPQMQWAWGRFPKVKQSERPKSEAQVGEQFSPTIQPSVSTHFRAIQRQDSFEMDVPPGESLEQDGSVTVLRPEPRLRPPGLQIPEERPHHEHPEPTHTTCSALGENTDLQQAPGILELDNAGPPLPETLHAEGISTPRPAQESRSETAEPSAETLAVQDAEKTTVCPQSSQATENVGVTESCEPGESSTGEAGLTSSETAMKPESQEKKKGKRSQHLGPADIYLDDLTSLNPEVAALYFPKSDSEMGVRGGADRGSLSGTQSPQSVGSGAMDSGTEYLSDSTSDMPEVTMSLCGRVVDTSLINKEKFMEHLVSFQDFVQNPGIIEDPNLVICINSKYYNWAVAAPMILSMQAFQKTLPKSAVDKLVKNKMPKKSGRWWFSWRRRDLDTKQPCGFRAGGSRTTAVLEPGLTGSRPRCLRAKQDGSSSEDDLGSARELRPGQPMSTSHCISQMYRKSLRLTSEQIARLNLREGANEIVFSVTTQYQGTCRCEAAIYLWNWDDKIIISDIDGTITKSDALGHILPQLGKDWTHRGVAKLFHKIHQNGYKFLYCSARAIGMADITKGYLQWVNDRGAVLPKGPVLLAPSSLFSALHREVIEKKPEVFKIACLTDIKNLFSPQKQPFYAAFGNRPNDVYAYKQVGVSESRIFTVNPKGELIQEQTKGNKSSYSRLSELVEHVFPVLIKGRSSSFDCPDFSQFSYWREPLPALDLRDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZV1</accession>
    <name>W5LZV1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011654">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZV1"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001663.1">
        <property type="protein sequence ID" value="ENSLOCP00000001658.1"/>
        <property type="gene ID" value="ENSLOCG00000001437.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102685461"/>
    <dbReference type="GeneTree" id="ENSGT00940000158526"/>
    <dbReference type="HOGENOM" id="CLU_012781_0_0_1"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001437">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009607">
        <property type="entry name" value="Enhancer_polycomb_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06752">
        <property type="entry name" value="E_Pc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of polycomb C-terminal" evidence="9">
        <location>
            <begin position="574"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="803" mass="90104" checksum="ED31694878B41931" modified="2014-04-16" version="1">MSKLSFRARALDAAKPLPIYRNRDLPDLTDCVSINRAVPQMPTGMEKEEESEHHLQRAISAQQVFREKKECMVIPVPEAESNITYYDRLYKGEFKIPKQLIHIQPFSLDNEQPDYDMDSEDETLLNRLNRKMEIKPIQFETMIDRLENASANQLVTLQEAKVLLNEDDCLLKSVYDYWVRKRKNCRGPSLIPQIKQEKRDGSTNSDPYVAFRRRTEKMQTRKNRKNDEASYEKMLKLRREFSRTVTILEMIKRREKSKRELLHLTWEVVEKRYQLGDFGGEILNEVKIPRAEKPVYSTPVSLHNGTHHKTSESKIKHSHHVSIKDESHFDFLRPKKKYSQKLKLDLLHQQSHSEHQPAVNKCDIKQYDFHSSGDEEYLQVPSPTSEPDEESDPDGPFAFRRKAGCQYHAPRVDQAGGVLWEQPELAGLRHRHCLTALTVPRRCIGFARRRVGRGGRVIMDRASSEHDHVLKQLDPEVLASTPSSMVSDYSDTSTWTNASRAHSGNVNSLTEILSNIKMCRWRYFRPQPTQAKNNGDNGSTFKKLGQTVNNKRITGASVALLSPNKNGITVAGGITEEEFHTHQQQLVQMQKQQLAQLQQKQQSQHSSHPEHHTIQVSGTSDCMSKTLDSASAQFAASAVVTAPGPGCSEASKDNRLHSISVNGVVQTSGTSKTLHSTSTTLSTTPAISSVQLLRTVPHSSTSHLIPALCTSTPQSLPGNSSCLATAVHLSNVSVVSPVNMHLSTRTSAPSPSALKLATVATPLDRVPKVTPSSAISSIARESHEPERLALNGISETTVAMEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M015</accession>
    <name>W5M015_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Testis specific, 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025109">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025110">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025111">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001722"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001727.1">
        <property type="protein sequence ID" value="ENSLOCP00000001722.1"/>
        <property type="gene ID" value="ENSLOCG00000001500.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RJHC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159205"/>
    <dbReference type="HOGENOM" id="CLU_008607_2_0_1"/>
    <dbReference type="InParanoid" id="W5M015"/>
    <dbReference type="OMA" id="DAKNEIH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001500">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF2">
        <property type="entry name" value="TESTIS SPECIFIC 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="99"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="760"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="141"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="398"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="720"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="767"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="811" mass="92865" checksum="B60EB0F6973CC53A" modified="2014-04-16" version="1">QREIAKLQETVKSLKAETESSKASCDRLQASLNASEQENGLLHSLLDRQQAEKAKLSKQVETLCLSEKEGVLGAESGRKPPRKTGSPSRLGSFVKSPEEDRDYFTQEAEAVRRRKRMLKNRTQSPTRSTSRGRGGSYDSELMRILRERDELQAMLDKYERHLSEIQANIKVLTADRDKTNMLYQQAQEEIARLRREVIKSPRAPRSSLTAQSILRRVEAERDETVADLRRMTNERDSLRERLKISQESAINERAHLEQRVEDLQSAFLILEKERGEQKTRQALMKDTMLSLEEEVRALSRRLDTTEEELTRTKTECSMLRYGGTHACTHPXQATQDKHKQLDQRNDTVICLMERALIDTSVEITSLREEVSTLQVTTAELDKEKDVLESELDDKIELISSLRGQLEEKNKSLQNLKLTIEEVEATASSTKEELSSREREVSSVRRQLDSVREELGAAGKARETLARECVQLREDLSKAKLENQALQLRLDDYGQEMEDLRLKMQDYSSDATRTQTLLESQEKEIREQQESCHRATAQAKSWEDKARQAEAEASSLRLELLSGDSERRRLKDKAEALEGSLEEALSAERVSRSQLSQLNRSLQRLEEELRQAQAERATAETELESTRDLCVKLDASKEAVNTKVCSAVARVRSVLGDLESYHADGELLRQQLASERLSMKNLESLLVANREKEFQIQLHTQEKQTEIQLLRDKLSLADSKAVTQSREVAQARSRASQLEAELEKTKRQLSTERFERERAVQELRRQGLSSSSPTRRSLSPRASWSPERSYLSPLDRSSLDRSPDRSVTFRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2W4</accession>
    <name>W5M2W4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2W4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002722"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002728.1">
        <property type="protein sequence ID" value="ENSLOCP00000002722.1"/>
        <property type="gene ID" value="ENSLOCG00000002323.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQVR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160300"/>
    <dbReference type="HOGENOM" id="CLU_191080_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2W4"/>
    <dbReference type="OMA" id="SCSAEIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002323">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052136">
        <property type="entry name" value="Adipolin/Erythroferrone-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019:SF14">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="76" mass="8127" checksum="3B04A9C8D30C5A2D" modified="2014-04-16" version="1">SQENVQMSSCSAEICRSFLSLEMIVGLESNSKIFTVYVHGLLELQAGQYTSIFVDNAAGASITIQSGSDFMGMLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M334</accession>
    <name>W5M334_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M334"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002792"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002798.1">
        <property type="protein sequence ID" value="ENSLOCP00000002792.1"/>
        <property type="gene ID" value="ENSLOCG00000002382.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="HOGENOM" id="CLU_086545_0_0_1"/>
    <dbReference type="InParanoid" id="W5M334"/>
    <dbReference type="OMA" id="NTANERM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002382">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF519">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867187" description="Receptor ligand binding region domain-containing protein" evidence="11">
        <location>
            <begin position="20"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="12">
        <location>
            <begin position="82"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="281" mass="31046" checksum="55D15967C63543DB" modified="2014-04-16" version="1">MNTHIVIFFMFSCGFQADGDLTCRILGKNTMSGLSKDGDLVIGGVFPVFGTLEDTGTLFTTAPQKAKCFGFDFDVFRWVQMMVFAIEEINMNNRLLPNITLGYRILDNCASPAETVRAGLILANGPEEVDSNSSCPSPVTAVITSNSLHIARAIGNFGIPLVSYTSTCVCLSNKRVFPTFSRTIPSDFFQAKAFAQLVKHFGWTWIGTIQADNEYGIFGIQSFTKEVVKYGVCIAFTENILSTYSRTKILQLVDIIKKSTVKVILAFASERDLYPLMQEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3B3</accession>
    <name>W5M3B3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger CCCH-type containing 18</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010622">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3B3"/>
    <dbReference type="FunCoup" id="W5M3B3">
        <property type="interactions" value="1635"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
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    <name>W5M608_LEPOC</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="914" mass="101637" checksum="265398BB441ACEC6" modified="2014-04-16" version="1">AAKRKVHFLLGLCLLFQVFAYLSAESTCKLKAKFNLYGYKNIEKKKVIVGGMFPVHFRLASSNSSTSSVPQSSDCEGFNFRTFRWLQTMRFAINEINNREDILPNTDLGYVIYDSCFTISKAVEGTLTYLTGQDEAVPNYRCSSGAPLAALIGAGASDLSIATARILGLYYFPQVSYASSCSVLESKFQFPSFLRTIPNDVHQSQAMARLVLHFGWTWVATIASDDDYGKYGIKVFKEQVEEAGVCISFSETLPKVYSRESVLRIVEAIKKSTANIIVVFSSDVDFSPLTEELVAHNITNKTWIASEAWITSALISKPEYNSALGGTIGFAIRRADIPGLKSYLLNLDPYEDRLTEEFWEKAFNCTLGYTRTVRSLNTSALSSNDTDTRIHNMDPVADHFCTGLESLERINNTYSDVSELRLTYSVYKSVYTVAHALHNLEHCEPGKGPFPMNSCADINNFEPWQLMYYLKNLRFTTHTNEDIFFDDNGDVKAEYDIINWQEKPDGSVSYVQIGHYNSTAPAESRMTINNDSIVWNNNILQAPRSVCSESCLPGTRKGIRQGEPVCCFDCIPCADGEISNETDARECIQCGEDYWSNTKRDACVPKEIEFLDFSEALGITLISISAFGACITIAVAIIFLVHRNTALVKANDRTLSFFLLLSLVITFLSSIVFIGRPQHWSCMTSQVALALGFAMCLSCIMGKTVVLMLRARAAKAKSQEQANGDPIKPIHQKSIALVCTLIQVCACTVWLILLPPYPVKNTASQNIKIILECNIGSIIFICCIFAYDILLALLGFICAFIARKMPDNFNEAKFVTFGMLVFFIVWISFVPAYLSTRGKFMVAVQIFAILASSFGLLACIFIPKCYIILIKPERNTEELVRGKSQKNDTSAPGTSVSISSELNNTTISTVSVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6L2</accession>
    <name>W5M6L2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fas apoptotic inhibitory molecule 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role as an inducible effector molecule that mediates Fas resistance produced by surface Ig engagement in B cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6L2"/>
    <dbReference type="FunCoup" id="W5M6L2">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004020"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004028.1">
        <property type="protein sequence ID" value="ENSLOCP00000004020.1"/>
        <property type="gene ID" value="ENSLOCG00000003397.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692354"/>
    <dbReference type="CTD" id="436856"/>
    <dbReference type="eggNOG" id="KOG4352">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007364"/>
    <dbReference type="HOGENOM" id="CLU_109086_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6L2"/>
    <dbReference type="OMA" id="SQEYRIM"/>
    <dbReference type="OrthoDB" id="6262731at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003397">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.180:FF:000001">
        <property type="entry name" value="Fas apoptotic inhibitory molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.180:FF:000002">
        <property type="entry name" value="Fas apoptotic inhibitory molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010695">
        <property type="entry name" value="FAIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038513">
        <property type="entry name" value="FAIM1_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13088:SF3">
        <property type="entry name" value="FAS APOPTOTIC INHIBITORY MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13088">
        <property type="entry name" value="FAS APOPTOTIC INHIBITORY MOLECULE FAIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06905">
        <property type="entry name" value="FAIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="22892" checksum="42FB79B153615F5D" modified="2014-04-16" version="1">MASREDFTLCEDEVRSRYGHLDRMTDLVGVWEVALSDGVHKIEFEHGTTSGKRVVYVDGKEIIRREWMFKLVGKETFNVGDTETKATINIDAVSGFGYEYTLEINGKSLKKFLENRSKTTNTWVINLDGVDCRIVLEKDTMDIWCNGKKMETAGEFVEDGTETHFTVGNHDCCIKAVSSGKRREGIIHSLIVDEMEIPEAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6N9</accession>
    <name>W5M6N9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6N9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004047"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004055.1">
        <property type="protein sequence ID" value="ENSLOCP00000004047.1"/>
        <property type="gene ID" value="ENSLOCG00000003422.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159805"/>
    <dbReference type="HOGENOM" id="CLU_005350_2_2_1"/>
    <dbReference type="InParanoid" id="W5M6N9"/>
    <dbReference type="OMA" id="CARRHTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003422">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF17">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="283" mass="33079" checksum="B5BE05C954BF262A" modified="2014-04-16" version="1">SKEVMDQNPGKFEALEREPGDPKWLDIIEKDLHRQFPFHEMFAARGGHGPAHTLRHCPCARRHTHTLKHCETLSPRTHTHRDTARHCPRAHTHTETLRDIVPLDGEIFFSLLRRVCPMAYRHLKKFKIDPILYMTEWFMCVYSRTLPWACVLRVWDMFFCEGVKIIFRVGLVLLRQMLGSVQQLRECQGMYETMERLRNIPPDNVKEEALVHEVISLPVTEALIERESKVQVKRWRETRGELQHQPCRRLHGTRAIQEQRLRSSHASLPPPAGPLRSSASLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7Y3</accession>
    <name>W5M7Y3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger DHHC-type containing 18</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7Y3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004491"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004499.1">
        <property type="protein sequence ID" value="ENSLOCP00000004491.1"/>
        <property type="gene ID" value="ENSLOCG00000003768.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156585"/>
    <dbReference type="HOGENOM" id="CLU_1408313_0_0_1"/>
    <dbReference type="InParanoid" id="W5M7Y3"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003768">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="193" mass="20148" checksum="60D16F7CDC045BDA" modified="2014-04-16" version="1">MCTLFSTGSQAGKGLIVALQESPASVLELVVCFFSVWSILGLSGFHTYLVASNLTTNEDIKGSWSGKRGAEDLGNPYSYNNIFKNCCAVLCGPMPPSLIDRRGFVPADSSPPAAPPEADLPPFVAKNDTNMPQWCPGPSQPTASTQRAAEGSSVRQTERQPPETALAGGSCAPHGVQGGELPGICALLHSLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M881</accession>
    <name>W5M881_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Inositol 1,4,5-triphosphate receptor associated 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01008208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M881"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004589"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004597.1">
        <property type="protein sequence ID" value="ENSLOCP00000004589.1"/>
        <property type="gene ID" value="ENSLOCG00000003841.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWGQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063722"/>
    <dbReference type="HOGENOM" id="CLU_018275_0_0_1"/>
    <dbReference type="InParanoid" id="W5M881"/>
    <dbReference type="OMA" id="MGVDLTC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003841">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008677">
        <property type="entry name" value="MRVI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRIPHOSPHATE RECEPTOR ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352">
        <property type="entry name" value="LYMPHOID-RESTRICTED MEMBRANE PROTEIN, JAW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05781">
        <property type="entry name" value="MRVI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="775"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="118"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="720"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="668"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="679"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="727"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="832" mass="91681" checksum="EDACA5D84E1200E7" modified="2014-04-16" version="1">MPTLPEEEEDSPEELDSSSSSPVTFTPVLTRMVPGPVSAPSIVLPRQGAPVDQDSKPLEQTRPHSPRPRLSRSSFSSGNPITTVDSEGHVIDLIKDQLPDVELSNEDKKKNLELLEEAKKVSERFLMRRGRRSICSLSESPTGLSPNPTPRSSPVPSRSSSLTVPPQIAPDGTEVNYVNPPINQHLEVPSPREKSDTRGQEPESPRKPLDRKPHEKRKVSQGTLPARLSAYEGSSEAAVEQKENCDPRLGGETRKKPPSSAGRAESEEPGRGSGRKHLESPDPPVGKGPVAPPLPSGACTAELKSIGCGPPLMRAVSWDSVGPVSIRNGTQAFPAKNDEPLSFYDKSNIALLKATGYKDFPVQPVRMQKLVQMREEHKLMRNQSIIGSKLPDLSETAEQNTGPSPNPTPGSPTEEEPRANSDVMPNISDVMLRKLKLHRALPTSAPPLTEKEVENAFVQLSLAFRNDSYTLESRLHQAERERNLTEENTEKELEDFKGFIKGSVLLWQNSEQRESYQRLLETVVVLHRLAVRLSSRAEMVGAVRQEKRMNKATEVMLQYVENLKRTYEKDHAELVEFKKLANQNSNRCYGSIDTGDDGVPRASRSMSLTLGKALPRRRVSVAVVPKFNLLNIPGQSPNSVGPGLPVLCEANSGKCSTPSDPVSAAGTENEKASRDPENETSSAPPSSNLDEIRSEIKAKIEEEAYNKGYEEGLKRSKELQELKEEEEKADESQEDAEEAEKEMTLEKKSSSRFEEALGVFNWLCPKIFRQNRLCWIIAIVVLVFAFIISVFTSFGHSYEELGEASSGNGGGSGKKLNFGWNVGLQPKNPTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M885</accession>
    <name>W5M885_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">protein deglycase</fullName>
            <ecNumber evidence="10">3.5.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">Maillard deglycase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">Parkinsonism-associated deglycase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="53 54">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="54" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="53" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="53" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>N(2)-(1-hydroxy-2-oxoethyl)-GDP + H2O = glycolate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57264"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:141574"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>N(2)-(1-hydroxy-2-oxoethyl)-GMP + H2O = glycolate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:141576"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>N(2)-(1-hydroxy-2-oxoethyl)-GTP + H2O = glycolate + GTP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:141571"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>N(2)-(1-hydroxy-2-oxoethyl)-dGTP + H2O = dGTP + glycolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:61429"/>
            <dbReference type="ChEBI" id="CHEBI:141572"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>N(2)-(1-hydroxy-2-oxopropyl)-GDP + H2O = lactate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57260"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:141573"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>N(2)-(1-hydroxy-2-oxopropyl)-GMP + H2O = lactate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57268"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:141575"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>N(2)-(1-hydroxy-2-oxopropyl)-GTP + H2O = lactate + GTP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57256"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:141570"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>N(2)-(1-hydroxy-2-oxopropyl)-dGTP + H2O = lactate + dGTP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57244"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:61429"/>
            <dbReference type="ChEBI" id="CHEBI:141569"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>N(6)-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] + H2O = glycolate + L-lysyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:14845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:141554"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>N(6)-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] + H2O = lactate + L-lysyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49552"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131709"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] + H2O = L-arginyl-[protein] + glycolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:14844"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:141553"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] + H2O = lactate + L-arginyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49548"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:12428"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:131708"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] + H2O = glycolate + L-cysteinyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:14846"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:141555"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] + H2O = lactate + L-cysteinyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49556"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:12430"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:131710"/>
            <dbReference type="EC" id="3.5.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N(2)-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14857"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:141579"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an N(2)-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14859"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:141581"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>an N(2)-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + lactate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57300"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:141578"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>an N(2)-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + lactate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57288"/>
            <dbReference type="Rhea" id="RHEA-COMP:14855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24996"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:141580"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.124" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01009744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642003.1">
        <property type="nucleotide sequence ID" value="XM_006641940.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M885"/>
    <dbReference type="FunCoup" id="W5M885">
        <property type="interactions" value="1280"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004601.1">
        <property type="protein sequence ID" value="ENSLOCP00000004593.1"/>
        <property type="gene ID" value="ENSLOCG00000003827.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699182"/>
    <dbReference type="KEGG" id="loc:102699182"/>
    <dbReference type="CTD" id="11315"/>
    <dbReference type="eggNOG" id="KOG2764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001231"/>
    <dbReference type="HOGENOM" id="CLU_000445_44_2_1"/>
    <dbReference type="InParanoid" id="W5M885"/>
    <dbReference type="OMA" id="KATCYPG"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003827">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036524">
        <property type="term" value="F:protein deglycase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046295">
        <property type="term" value="P:glycolate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903189">
        <property type="term" value="P:glyoxal metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001895">
        <property type="term" value="P:retina homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03135">
        <property type="entry name" value="GATase1_DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000057">
        <property type="entry name" value="Protein/nucleic acid deglycase DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006287">
        <property type="entry name" value="DJ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01383">
        <property type="entry name" value="not_thiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF12">
        <property type="entry name" value="PARKINSON DISEASE PROTEIN 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0072">Autophagy</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="29" id="KW-0143">Chaperone</keyword>
    <keyword evidence="22" id="KW-0186">Copper</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="32" id="KW-0278">Fertilization</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="30" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="13" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="33" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="26" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="31" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-0558">Oxidation</keyword>
    <keyword evidence="27" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0645">Protease</keyword>
    <keyword evidence="54" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="23" id="KW-0346">Stress response</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="28" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="52">
        <location>
            <begin position="4"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00030958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00031312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00047717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00047824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00048443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00048587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00048622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00048786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00048884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00048937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00048993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00049074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="ARBA" id="ARBA00049200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00049222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="50">
        <source>
            <dbReference type="ARBA" id="ARBA00049281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="51">
        <source>
            <dbReference type="ARBA" id="ARBA00049474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="52">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="53">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="54">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="189" mass="19802" checksum="53CD3EDFD039C11E" modified="2014-04-16" version="1">MAAKRALVILSKGAEEMETVIPVDIMRRAGVSVTLAGLSGKEPVQCSRDVLICPDASLEEARAQGPYDVVVLPGGNLGAQNLSESPVVKDVLKDQEGRKGLIAAICAGPTALLAHGIAYGSTVTTHPLAKDKMMNGEHYKYSEARVQKDGNLITSRGPGTSFEFALAIVEALLGPEVAAQVKAPLVLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M960</accession>
    <name>W5M960_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mesothelin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M960"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004927.1">
        <property type="protein sequence ID" value="ENSLOCP00000004919.1"/>
        <property type="gene ID" value="ENSLOCG00000004114.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRX1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182957"/>
    <dbReference type="HOGENOM" id="CLU_969629_0_0_1"/>
    <dbReference type="InParanoid" id="W5M960"/>
    <dbReference type="OMA" id="QTHTIFR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010335">
        <property type="entry name" value="Mesothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026664">
        <property type="entry name" value="Stereocilin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412:SF6">
        <property type="entry name" value="MESOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412">
        <property type="entry name" value="STEREOCILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06060">
        <property type="entry name" value="Mesothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="287" mass="31659" checksum="4F736BE4ADD169D1" modified="2025-10-08" version="2">MSQLLSTMFSMTSNRGLLTPLKTCCVMETGTLKYLSSLIIQTHTIFRNYSSWNIKTLENLGILSLYLSSDFWCPIKIPDLQKFLKHFIPQQRKNKALPKVPYCYYVISISKSLQSDFLNLTMQFSFEASCTADNITLATINDGAFPVGYDLTQFDLCLGVSIARDDLAALTGKVDDENGQMIIMAKLNEVYPSVAPEDQVQLFGPTFHQASLNEISRWTITKLDTLAALVDSTNGAWDSTKAKAIITKYLASGNNSLGSAELNSIGGTNLCSLDISVLETIATDSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9F8</accession>
    <name>W5M9F8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Solute carrier family 10 member 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9F8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005017"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005025.1">
        <property type="protein sequence ID" value="ENSLOCP00000005017.1"/>
        <property type="gene ID" value="ENSLOCG00000004197.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694983"/>
    <dbReference type="KEGG" id="loc:102694983"/>
    <dbReference type="eggNOG" id="KOG2718">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182808"/>
    <dbReference type="HOGENOM" id="CLU_034788_7_5_1"/>
    <dbReference type="InParanoid" id="W5M9F8"/>
    <dbReference type="OMA" id="CLYLYTW"/>
    <dbReference type="OrthoDB" id="203097at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004197">
        <property type="expression patterns" value="Expressed in mesonephros and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008508">
        <property type="term" value="F:bile acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015721">
        <property type="term" value="P:bile acid and bile salt transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000010">
        <property type="entry name" value="Solute carrier family 10 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00841">
        <property type="entry name" value="bass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF55">
        <property type="entry name" value="SODIUM-DEPENDENT ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="372" mass="40461" checksum="BF97E6AF1BBCAA30" modified="2014-04-16" version="1">MLNHTGCSECEGNRTHNGTSAPDETVTLVFSVVLTALLALVVFSLGCTVELGKLWFHLRRPWGILVGLVCQFGLMPLTAYALAVGFAVKPVQAVAILIMGCCPGGTISNIIAYWVDGDMDLSITMTCCSTVLAMGMMPLCLFIYTRSWVGEGNIKIPFFNIGITLVTLIVPVCCGVFVNYKWPKKAQIILKVGTVVGGVLILVIGIASAVLYKGSWDTDTSMLVIGIIYPMIGYAAGFITAAVVRQPWKRCRTIALETGAQNVQVCSTMLQLSFPAEQLVLMFTFPLIYGSFQLLNGLLIVTVYQIYKKVTSDKPDDKPLAGPGLINSLSFKTPQGEVNMAFENEKALCEVNKQSLEPVKAEERQMMECTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9L0</accession>
    <name>W5M9L0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peroxisomal N(1)-acetyl-spermine/spermidine oxidase</fullName>
            <ecNumber evidence="16">1.5.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Polyamine oxidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1),N(12)-diacetylspermine + O2 + H2O = 3-acetamidopropanal + N(1)-acetylspermidine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="ChEBI" id="CHEBI:58550"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(1)-acetylspermidine + O2 + H2O = 3-acetamidopropanal + putrescine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(1)-acetylspermine + O2 + H2O = 3-acetamidopropanal + spermidine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25800"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amine and polyamine metabolism; spermine metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.3.13" evidence="16"/>
    <dbReference type="EMBL" id="AHAT01025215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9L0"/>
    <dbReference type="FunCoup" id="W5M9L0">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005069"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005077.1">
        <property type="protein sequence ID" value="ENSLOCP00000005069.1"/>
        <property type="gene ID" value="ENSLOCG00000004236.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158274"/>
    <dbReference type="HOGENOM" id="CLU_004498_2_3_1"/>
    <dbReference type="InParanoid" id="W5M9L0"/>
    <dbReference type="OMA" id="GTHSMDE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004236">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052903">
        <property type="term" value="F:N(1)-acetylpolyamine oxidase (3-acetamidopropanal-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046203">
        <property type="term" value="P:spermidine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000008">
        <property type="entry name" value="Spermine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF405">
        <property type="entry name" value="PEROXISOMAL N(1)-ACETYL-SPERMINE_SPERMIDINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="19">
        <location>
            <begin position="27"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="514" mass="56676" checksum="A5F88E230BB97B97" modified="2014-04-16" version="1">TQCSGTQISKFGRKGVDSHIVIVGCGISGIGAAQKLIKHGFKNVRILEATQRSGGRIKTTRLGNKIVEIGANWIHGPSKENPVFRLACRYRLLDEEALSEQNQALDVGGHPPFKPNWFCSSGGKLGPDVTEPVAALFASLLQQSQQFHAEQREPVPSVGEFLKGELARHAAQWGDGPAARRLKLAVASVLLKLECSISGAHSMDCVGLGAYGLYETLPGLDCTFPRGYEGLIDAMMKELPRGIVSYSRPVRCIHWDGAFRRAGKTFPVLIECENGETFPANHVIVTVPLGYLKKHQDTLLSPALPLGKAHAIQRMGFGTNNKIFLEFDEPFWDPDCEVIYFVWDDESPVTDVVPDVQKYWMKKLFGFTVLRPTEKYGHLLCGWIAGEESEYMETLSEEEVKQSITQVIRRFTGNPVITPKRILRSQWHLDPYTCGSYSYVAVGCSASDIDSIAEPLPLKQSQSEPLQVLFAGEATSKSFFSTTHGALLAGWREADRLIAHHTTSPPSAPLNSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9P2</accession>
    <name>W5M9P2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Enoyl-CoA hydratase, mitochondrial</fullName>
            <ecNumber evidence="5">4.2.1.17</ecNumber>
            <ecNumber evidence="4">5.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Enoyl-CoA hydratase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Short-chain enoyl-CoA hydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45736"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:84790"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:45737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26558"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:26560"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31191"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:31193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:30547"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62075"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:30549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:31175"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62500"/>
            <dbReference type="ChEBI" id="CHEBI:62611"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:31176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45584"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:78611"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:45586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-hydroxyisovaleryl-CoA = 3-methylbut-2-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31079"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:62555"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:31081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26518"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57367"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:26520"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:16107"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homohexamer; dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="5"/>
    <dbReference type="EC" id="5.3.3.8" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01025214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9P2"/>
    <dbReference type="FunCoup" id="W5M9P2">
        <property type="interactions" value="1406"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005101"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005109.1">
        <property type="protein sequence ID" value="ENSLOCP00000005101.1"/>
        <property type="gene ID" value="ENSLOCG00000004265.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157609"/>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_1"/>
    <dbReference type="InParanoid" id="W5M9P2"/>
    <dbReference type="OMA" id="FCDARED"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004265">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000213">
        <property type="entry name" value="Enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="28" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865849" description="Enoyl-CoA hydratase, mitochondrial" evidence="28">
        <location>
            <begin position="22"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035854"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00041421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="31665" checksum="9A439519ECCA6437" modified="2014-04-16" version="1">ISIKMAALCRTAALLLKPAKALPYTFVAAQNCSSGGHYQYILVEKKGSQQNVGFIQLNRPKALNALCDGLMTEMGQALDAFEADSQIGAIVITGSEKAFAAGADIKEMQNRTFQECYSGNFLAHWNRLSTVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAHFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQEAKASGLVSKVYPADQVVQEAIRCGEKIAENSKLVSAMAKEAINAAFELTLAEGNRLEKRLFHATFATDDRKEGMTAFVEKRKASFKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9S9</accession>
    <name>W5M9S9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterohexamer; composed of 3 copies of TIMM8 (TIMM8A or TIMM8B) and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639907.1">
        <property type="nucleotide sequence ID" value="XM_006639844.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9S9"/>
    <dbReference type="FunCoup" id="W5M9S9">
        <property type="interactions" value="1762"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005138"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005146.1">
        <property type="protein sequence ID" value="ENSLOCP00000005138.1"/>
        <property type="gene ID" value="ENSLOCG00000004301.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698509"/>
    <dbReference type="KEGG" id="loc:102698509"/>
    <dbReference type="CTD" id="26517"/>
    <dbReference type="eggNOG" id="KOG1733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014000"/>
    <dbReference type="HOGENOM" id="CLU_141397_0_2_1"/>
    <dbReference type="InParanoid" id="W5M9S9"/>
    <dbReference type="OMA" id="MAAWNQV"/>
    <dbReference type="OrthoDB" id="7813104at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004301">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="mitochondrial import inner membrane translocase subunit TIM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
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        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="12">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="96" mass="10693" checksum="B99DE8B487A8CEDC" modified="2014-04-16" version="1">MDSFGSDFSSVSSGSGKVDTGTIMEQVKVQIAVANAQELLQRMTDKCFKKCIGKPGGTLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA23</accession>
    <name>W5MA23_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat and kinase domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA23"/>
    <dbReference type="FunCoup" id="W5MA23">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000005240.1">
        <property type="protein sequence ID" value="ENSLOCP00000005232.1"/>
        <property type="gene ID" value="ENSLOCG00000004369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683077"/>
    <dbReference type="KEGG" id="loc:102683077"/>
    <dbReference type="CTD" id="255239"/>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162060"/>
    <dbReference type="InParanoid" id="W5MA23"/>
    <dbReference type="OMA" id="PTHQGWT"/>
    <dbReference type="OrthoDB" id="195446at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004369">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046959">
        <property type="term" value="P:habituation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010564">
        <property type="term" value="P:regulation of cell cycle process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF175">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="396"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="429"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="495"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="561"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="594"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="627"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="660"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="693"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="751" mass="84683" checksum="403F938E4354EF6C" modified="2014-04-16" version="1">MEQSSEETKYFSKDDFEADWVKIASRNFSQVFKVKVKVWRETFAIKCFPNMSSDTSLYRQMMKEASKMEKMKFSYIVSVYGICNDPMAIVMEYMSNGSMDSLLTSHLLMWPKKFQMIHEVTMGMNFLHSVKPPLLHLNLKPANILLDDHLHVKISDFGLVRWEETSSRTEFIEQAAARGNISYIPPEMFLESAKSPGTKYDVYSFAIVTWEIIAQVKPYPGTNRMAVIMRVASGKRPSLEKIPEDRPDQCNQLIDLMERCWNQDLRQRPCFSEIVTETEELREILKIPDLRRGSDEKSRSSQPVYSKMSTNLKEVEVIDMKPRFKDTDEINSCKDIIGLLSRREFMDFKKMLNKEHVSMVFEDGNSLLHYAVAMGDLETVKLVLHFGATVNSQSIKGYTPLIIAVIYKFLDICSLLLEHGADVNLADKDQWTPLHFAVQSGEDRITRLLLDSYAQVNARERDGWTPLHLASQNGYESMVRTLLTRKVTVDQQENSGRTALHISCAYGHVNIAKLLIGHQADLNERDNGNCTPLHLASDEGHFRVTRLLIKSGADVNIVDQNRSTALHLAALKGHNGSCRQLLAQGAIPDLQTSQGWTPLHLASLKGHPTTVQILVEHHATVNLQGENGQTPLHIACHYGQDDVVSELLRAEADPNIAEDSGWTPLHVACHRGNLSCVQQLISHQVNINAQNNSDRTPLHLAVLSGSTSTVRELLMSGADQTTKDATGCTALRLAIDNLKEDIAELLLRKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAB6</accession>
    <name>W5MAB6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tumor protein p53 inducible nuclear protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01025258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAB6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005325"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005333.1">
        <property type="protein sequence ID" value="ENSLOCP00000005325.1"/>
        <property type="gene ID" value="ENSLOCG00000004455.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZHB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063829"/>
    <dbReference type="HOGENOM" id="CLU_091034_0_0_1"/>
    <dbReference type="InParanoid" id="W5MAB6"/>
    <dbReference type="OMA" id="NGPKPCV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004455">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029431">
        <property type="entry name" value="TP53INP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671">
        <property type="entry name" value="DIABETES AND OBESITY REGULATED, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671:SF2">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE NUCLEAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14839">
        <property type="entry name" value="DOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="225" mass="24732" checksum="23AE074D850BD207" modified="2014-04-16" version="1">MFQRLTSLFFGEVEEVYKECSGPKPCISEADEDDWLLVSLPGSYSHSRGRGLSCPFPCPRGSTPLSPGCQSDCSSGFERCCMDESWFVTPPPCFTAEGSTPEASPMEDLLIEHPSMSVYVSTGNQSVAEETGSLVLQETSQRVDPAVSAGRALSSRPPRGSASQAAALAKVAQVSRVQRAKARSERRQLGRHRLQRQNRLRDLPRHSGPARGGFLHQPCQRRFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MC67</accession>
    <name>W5MC67_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pyroglutamylated RF-amide peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">AQ27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">G-protein coupled receptor 103</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Orexigenic neuropeptide QRFP receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SP9155</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the orexigenic neuropeptide QRFP. The activity of this receptor is mediated by G proteins that modulate adenylate cyclase activity and intracellular calcium levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025169">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MC67"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005976"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005984.1">
        <property type="protein sequence ID" value="ENSLOCP00000005976.1"/>
        <property type="gene ID" value="ENSLOCG00000004968.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286926"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_3_1"/>
    <dbReference type="InParanoid" id="W5MC67"/>
    <dbReference type="OMA" id="CCQESWS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004968">
        <property type="expression patterns" value="Expressed in zone of skin and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15205">
        <property type="entry name" value="7tmA_QRFPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000227">
        <property type="entry name" value="Pyroglutamylated RFamide peptide receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF20">
        <property type="entry name" value="PYROGLUTAMYLATED RFAMIDE PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="70"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="441" mass="50378" checksum="4968B856C010739B" modified="2014-04-16" version="1">LRNKMTDSSEMRKITPDALKQLLQFYNLTRQDFIQTYNLQPLVYIPELPSGAKTTFVVMYAIIFVLALVGNSLVVYIIVKKRAMRTATSIFICSLAVSDLLITFFCIPFTLLQNISSEWLGGVFVCKTVPFVQSTAIVTGILTMTCIAIERYQGIVYPLKMKRQYTPKRAYKMLGAVWTVAVIVGSPMLYVQQLEVKYDFLYDHHHVCCQERWRSSAHRQVYATFILVALFILPLTAMLILYSRIAFELWVRKRVGDSSVLNTMNHSEINKIARKKKRAVKMMITVVLLFTICWAPFHVVHMLFEYNDFEKEYDDVTVSMIIAIVQAVGFFNSFNNPIVYAFMNENFKKNCLSTLSRCVRRRNPSRQRAVSAERPKPNVLFTNATRRGRFQGGTPTQGIQFNLSEEAVSASSHAVQSTAFAEEKISTVHCELPTSSCSYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCL4</accession>
    <name>W5MCL4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Oxaloacetate tautomerase FAHD1, mitochondrial</fullName>
            <ecNumber evidence="5">3.7.1.5</ecNumber>
            <ecNumber evidence="2">4.1.1.112</ecNumber>
            <ecNumber evidence="9">5.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Acylpyruvase FAHD1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Fumarylacetoacetate hydrolase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Oxaloacetate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-fumarylpyruvate + H2O = fumarate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26168"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16854"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3-acylpyruvate + H2O = a carboxylate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19009"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57278"/>
            <dbReference type="EC" id="3.7.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>acetylpyruvate + H2O = acetate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16097"/>
            <dbReference type="ChEBI" id="CHEBI:15360"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="4.1.1.112"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>oxaloacetate = enol-oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:16021"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="EC" id="5.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:16023"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.5" evidence="5"/>
    <dbReference type="EC" id="4.1.1.112" evidence="2"/>
    <dbReference type="EC" id="5.3.2.2" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01013490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCL4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006123"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006131.1">
        <property type="protein sequence ID" value="ENSLOCP00000006123.1"/>
        <property type="gene ID" value="ENSLOCG00000005085.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160452"/>
    <dbReference type="HOGENOM" id="CLU_028458_5_0_1"/>
    <dbReference type="InParanoid" id="W5MCL4"/>
    <dbReference type="OMA" id="NCRKVIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005085">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047621">
        <property type="term" value="F:acylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="15">
        <location>
            <begin position="21"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="224" mass="24825" checksum="48B9CC0CF1F9C504" modified="2025-10-08" version="2">MGFEGMASARNLNRFWEWGRKIICVGRNYADHAKELKNAIPSEPVLFLKPPSAYVREGSPILLPVYSTNLHHEVELGVVIGRGGTAIPQEAAMDHVAGYALCLDMTARDVQEECKSKGLPWTLAKAFNTSCPVSEFIPRDRVPDPGNVRLWLKVNDQLRQEGSTSQMIFSIPFLISYISEVITLEEGDLILTGTPRGVSSVQEHDEVQAGIEGVVSMTFRVDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDR8</accession>
    <name>W5MDR8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spindlin-1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIN/STSY family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639926.1">
        <property type="nucleotide sequence ID" value="XM_006639863.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220996.1">
        <property type="nucleotide sequence ID" value="XM_015365510.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220997.1">
        <property type="nucleotide sequence ID" value="XM_015365511.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220998.1">
        <property type="nucleotide sequence ID" value="XM_015365512.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015220999.1">
        <property type="nucleotide sequence ID" value="XM_015365513.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221000.1">
        <property type="nucleotide sequence ID" value="XM_015365514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDR8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006527"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006535.1">
        <property type="protein sequence ID" value="ENSLOCP00000006527.1"/>
        <property type="gene ID" value="ENSLOCG00000005412.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685080"/>
    <dbReference type="KEGG" id="loc:102685080"/>
    <dbReference type="eggNOG" id="ENOG502QY0H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182925"/>
    <dbReference type="HOGENOM" id="CLU_068595_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDR8"/>
    <dbReference type="OMA" id="HYWREKG"/>
    <dbReference type="OrthoDB" id="9944558at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005412">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.70:FF:000001">
        <property type="entry name" value="Spindlin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.70">
        <property type="entry name" value="Spindlin/Ssty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003671">
        <property type="entry name" value="SPIN/Ssty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042567">
        <property type="entry name" value="SPIN/Ssty_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10405">
        <property type="entry name" value="SPINDLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02513">
        <property type="entry name" value="Spin-Ssty"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="235" mass="27176" checksum="405113F7CE956BDC" modified="2014-04-16" version="1">MSKKKGRKRSSGELSDTLTPDPGSILGVRIQHNWREKGALTKWKGTVLDRLSVNPSLFMVKYDGFDCVYGIELFKDDRVSGLQVLTEKVVNNKIKVPHDAEELVGKAVEHLFEKEDGEKNEWRGMVLSRAPIMTNWYYITYEKDPVLYMYQLWDDYEEGDLRILPEAENRHLLPADRKPGEETESLVGKQVEYVTDKGVKRTGLVIYQVPAKPSVYYIKYDDDFHIHVYDLVKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDS5</accession>
    <name>W5MDS5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Borealin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the borealin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034964">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDS5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006534"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006542.1">
        <property type="protein sequence ID" value="ENSLOCP00000006534.1"/>
        <property type="gene ID" value="ENSLOCG00000005416.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S23P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005970"/>
    <dbReference type="HOGENOM" id="CLU_1128756_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDS5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005416">
        <property type="expression patterns" value="Expressed in ovary and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018851">
        <property type="entry name" value="Borealin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018867">
        <property type="entry name" value="Cell_div_borealin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040">
        <property type="entry name" value="AUSTRALIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040:SF10">
        <property type="entry name" value="BOREALIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10444">
        <property type="entry name" value="Nbl1_Borealin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Borealin N-terminal" evidence="12">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="246" mass="27808" checksum="3780435926B6B49F" modified="2014-04-16" version="1">MAPRKWKKNFSAAEVEGVSQLNNEQREQKTKLFMQQFQKEAQERTKEMEEKLEQTVATVEKAFKVQILKMPPSLQRKSVKELTSEDESQAGEVTIAMQADSSVIEPVKKKIGKKAQIANKSEASRPKQLRRILSVQSNKTVETVGKTVTKSRSLDFSNSTCARPTPPRASPAAPASTLRTRRTHSRIAKATNLTALPSESLLRFPPLFFSNCFLNLVVHFFFPYLMCTFVLSFLSSCSLWLVRYAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDY1</accession>
    <name>W5MDY1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Proton-coupled folate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Solute carrier family 46 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate(in) + H(+)(in) = (6S)-5-methyl-5,6,7,8-tetrahydrofolate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>folate(in) + H(+)(in) = folate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:62501"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>methotrexate(in) + H(+)(in) = methotrexate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50681"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>pemetrexed(in) + H(+)(in) = pemetrexed(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70171"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63724"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Basolateral cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the major facilitator superfamily. SLC46A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDY1"/>
    <dbReference type="FunCoup" id="W5MDY1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000006598.1">
        <property type="protein sequence ID" value="ENSLOCP00000006590.1"/>
        <property type="gene ID" value="ENSLOCG00000005466.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183096"/>
    <dbReference type="HOGENOM" id="CLU_028365_1_1_1"/>
    <dbReference type="InParanoid" id="W5MDY1"/>
    <dbReference type="OMA" id="KRSECGN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005466">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF2">
        <property type="entry name" value="PROTON-COUPLED FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="24" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867676" description="Proton-coupled folate transporter" evidence="24">
        <location>
            <begin position="27"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="48178" checksum="2558C67C111BDE53" modified="2014-04-16" version="1">RPPFPCRLPVTVEPVLFLCTFSLVLQTPLCTQYLWDRISEDLGYNGTREGGCRNDSGPQTLQTEVETLTAHWNLYISLGGFVVGVFTATLMGSWSDRAGRRPVLMLPSLALALQAGVYLLVMYLRLPVAWFLLGRLLSGLSGDFNAVLAACFAYVADVSDARSRTFRVAVLEACIGVAGMLASVVGGQWRLAQGYINPFWLVLATAMAAALYAYLFVPESVRPDPGARLFSMEHYRAVGRLCAANGGGGRRSRLWLYLLSFFVVVTVHFGSRGLYVIYELSAPLCWGPVLIGYGSAAQYLSSLTSLLGLRALQWCLEDSWIAVIGLASNMASLVVIAVASTTTLMFTGRGQMTCFLFSNPENFAHSWTSKCIEPVKLGALFALVACVESLCMLVSNGVFNSLYPATLHFLKGFPFLFGAGLLLVPAAIIGTLKLREKTSDYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME97</accession>
    <name>W5ME97_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Natriuretic peptide B</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the natriuretic peptide family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ME97"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006706"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006714.1">
        <property type="protein sequence ID" value="ENSLOCP00000006706.1"/>
        <property type="gene ID" value="ENSLOCG00000005558.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107075561"/>
    <dbReference type="KEGG" id="loc:107075561"/>
    <dbReference type="eggNOG" id="ENOG502S9RQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154513"/>
    <dbReference type="HOGENOM" id="CLU_126636_0_0_1"/>
    <dbReference type="InParanoid" id="W5ME97"/>
    <dbReference type="OMA" id="KLMSHIW"/>
    <dbReference type="OrthoDB" id="428525at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005558">
        <property type="expression patterns" value="Expressed in heart and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051427">
        <property type="term" value="F:hormone receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036302">
        <property type="term" value="P:atrioventricular canal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905072">
        <property type="term" value="P:cardiac jelly development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060038">
        <property type="term" value="P:cardiac muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003085">
        <property type="term" value="P:negative regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000663">
        <property type="entry name" value="Natr_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030480">
        <property type="entry name" value="Natr_peptide_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050787">
        <property type="entry name" value="Natriuretic_peptide"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14066">
        <property type="entry name" value="ATRIAL NATRIURETIC FACTOR PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14066:SF10">
        <property type="entry name" value="NATRIURETIC PEPTIDES B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00212">
        <property type="entry name" value="ANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00183">
        <property type="entry name" value="NAT_PEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00263">
        <property type="entry name" value="NATRIURETIC_PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0838">Vasoactive</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866040" evidence="8">
        <location>
            <begin position="25"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="144" mass="16319" checksum="D0CEB4A8211D8145" modified="2014-04-16" version="1">MQVLTDLSFFYALLLLLNAQLCSAQPFHTDTVAKDVENLKNLLQRLEEAFPSPDEQEPFLSEQSEQGVFDDLPGEAQSEQPRDELHPKTTDLEKYLSARDLQTLRSAHSSSKRYSGCFGRRLDRIGSMSSLGCNTSSRYRSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEW0</accession>
    <name>W5MEW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Persulfide dioxygenase ETHE1, mitochondrial</fullName>
            <ecNumber evidence="13">1.13.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sulfur dioxygenase ETHE1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-sulfanylglutathione + O2 + H2O = sulfite + glutathione + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12981"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58905"/>
            <dbReference type="EC" id="1.13.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Monomer. Interacts with TST. May interact with RELA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.18" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01019570">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEW0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006919"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006927.1">
        <property type="protein sequence ID" value="ENSLOCP00000006919.1"/>
        <property type="gene ID" value="ENSLOCG00000005717.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0814">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159046"/>
    <dbReference type="InParanoid" id="W5MEW0"/>
    <dbReference type="OMA" id="VMDIDYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005717">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000013">
        <property type="entry name" value="Persulfide dioxygenase ETHE1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="16">
        <location>
            <begin position="75"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="302" mass="33133" checksum="EE96F94824B1FC85" modified="2014-04-16" version="1">MSSAVLRTSKPALGFASLVRLAGQSNGSQAGLTRTLLGSLSSCHPLHRRSWRHYSVRMDQRTGLLFRQLFESQSSTYTYLLADAQTREAVLIDPVLETVERDARLVEDLGLNLTIADPVNTHCHADHITGTGLLKKRLPGCHSAISKDSGATADIHLKEGDRVTFGKYSLEARATPGHTDGCMTFVLNDQSMAFTGDALLIRGCGRTDFQQGCASALYESVHKKIFTLPGECLVYPAHDYTGQSVSTVDEERRLNPRLTKSLEEFVKIMNNLNLPKPKQIDVAVPANLVCGIQNDHYCADCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFX4</accession>
    <name>W5MFX4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="13">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01004477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MFX4">
        <property type="interactions" value="1354"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007283"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007291.1">
        <property type="protein sequence ID" value="ENSLOCP00000007283.1"/>
        <property type="gene ID" value="ENSLOCG00000006026.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286951"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="InParanoid" id="W5MFX4"/>
    <dbReference type="OMA" id="AFFKCNV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006026">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001878">
        <property type="term" value="P:response to yeast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000001">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="273"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="678" mass="76999" checksum="F24B8EC1EBA4DD73" modified="2014-04-16" version="1">RFLQRFIDPTSREDENAGLDLNEPLYMQKLEEINVVGEPVLNVNCAHLQSFDADLYRQLVCYPQEVIPTFDMAVNETFFERFPDSILEYQIQVRPYNAQKTKNMRSLNPEDIDQLITISGMVIRTSQLIPEMQEAFFRCQVCAFSTRVEVDRGRIAEPAVCRNCNTTHSLALIHNRSVFSDKQMIKLQESPEDMPAGQTPHTTVLXGIYRAVPMRLNPRQSNVKSVYKTHIDAIHFRKTDEKRLHGLDEDLEQKLFTEERVQTLKELAAKPDVYERLSSALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGNFRAEVNILLCGDPGTSKSQLLQYVFNLVPRGQYTSGKGSSAVGLTAYITKDPETRQLVLQTGALVLSDNGICCIDEFDKMSDNTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDDAYDRRLAHHLVSLYYQTEEQIEEEFMDMAVLKDYIAYARTYINPRLSEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLAEAHAKVRFSEVVETIDVEEAKRLHREALKQSATDPRTGFVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAMKYQQLFDDLRGQSDVAITKDMFDEALRALGDEDFLTVTGKTVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG00</accession>
    <name>W5MG00_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG00"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007309"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007317.1">
        <property type="protein sequence ID" value="ENSLOCP00000007309.1"/>
        <property type="gene ID" value="ENSLOCG00000006054.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="HOGENOM" id="CLU_002753_3_9_1"/>
    <dbReference type="InParanoid" id="W5MG00"/>
    <dbReference type="OMA" id="NSICWIT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006054">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000222">
        <property type="entry name" value="Adhesion G protein-coupled receptor G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF454">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="94"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="266"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="566" mass="63148" checksum="7037136E41E01F6B" modified="2014-04-16" version="1">MQISSNQGNSFFTVASENWNTSSTCNITVCNESDITNTLNSLKNNPNVLDVIQKLPILKKSCNFTEDLNNTYTYNEIQKKIIQNVMNMDFSGNNITVTTPEVAIVVYKMKITSTTPQEIDIAMPQMLNYRSILLSLKKTLSKNNNNTEKRVAVACFESADQFKTAAGEEVRSMVIQIEVVGETISGLKNPLTLNFPLYNKTDYNANYTETCKFYNSGAKRESVWSTDGCNTNKTNTTVICTCNHMTPFAVLMVPVGKISEPDWQILSVLSYIGCGLSAGFTAISVLTYFILRSPKRDHSYTIHVCLSAALYMLNMTFLLNEWLTSKAMSTLCTFIAVFMHYSLLSCFTWMAIEAVHLYLLMIKVFNTYIRHYISKLSVAGWVTGIPAVIVGISMALNGKYEIYGVLSTSLITSNASASTNRCWIMNQNFLYGVNIAYFGIIFLFNMGILITVAVKIGQLRRFGKSSDKGVLWKDMCTVTGLTCLLGTTWGLAFFGHGVFTTPVLYMFTIFNSLQGFFIFLWTCASSSKGVKTNDFKTKSSYAGCETSSIAKPKEVLFPELNSDPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGJ3</accession>
    <name>W5MGJ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkeyp-7a3.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGJ3"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007510.1">
        <property type="protein sequence ID" value="ENSLOCP00000007502.1"/>
        <property type="gene ID" value="ENSLOCG00000006212.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502R7DC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166855"/>
    <dbReference type="InParanoid" id="W5MGJ3"/>
    <dbReference type="OMA" id="AICHIQD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006212">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038826">
        <property type="entry name" value="CCDC178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="390"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="792" mass="91445" checksum="652791F27A857B22" modified="2014-04-16" version="1">MPEVELLRFSSKEGECQFQGETNDNFSKALVSSFPTAKVTSETIQKPQENSDNGEENDLQRVCSLRRRSCALVNTPSPCVNKAIHHVQELERKIEGWIQQMEREVSPQTNHSGKIVRFSTTEGNTAPPIITSLVSPGLCIEGKELCVREGGTSTPLNAETGAVLLEVIDLLGRLEADRQEAEEALKKEKERRRALGEKIDRLSLWRLQQLPAAVQKEHEACVRDISELQWHLKFKKERLQHVKDRMTKAEVLNQRLCEDIDFVKKHGPLLKGKLEMESSIMNQIKCAQDEANETLSKAVLDLGNAQQHFEEEQLRAATERNGMAKELDGIADQLKGILKDLQQLKHHWNAYSSKIAEIEEKLTLKEKICEYLLKQIPHLREQEITMNGEAEELRLKIEDQMLKIQQLEEDTSELQRQIQVTSSMGEAEVRQLEQEYNQKRQALLALSEKNRECELETEDFTRHIQQSEQSTVQLQKDRKRMLQKISQNEEQRDRVKEELSQVAAAHNATKAKLEDHEQQIYVEEQRMREVAENMKRQIMSEMKAIAILKGKIASEKTELQQGQTNTEKEKVEIQKAFEDSSSSILELEKEVEEFQRMYNEKKECIENMKKNIQELEVQHKTTAEKLEQKKTTLLHHLRDVENRHAAVSAELTHVLSRIEDLSRKTEEYRVSKCVMEKTARTMPDAIEKLQADFDAVDFKHKTATSIINSLQSEISNCKDRIKLSEETHKSLYKNRRITMEETKVRKGLSHPLSVNAFKSQVKIGCFCGSTLLNSQTIPCHFYINPFNEMLWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH48</accession>
    <name>W5MH48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01020936">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH48"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007707"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007716.1">
        <property type="protein sequence ID" value="ENSLOCP00000007707.1"/>
        <property type="gene ID" value="ENSLOCG00000006377.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00880000138014"/>
    <dbReference type="InParanoid" id="W5MH48"/>
    <dbReference type="OMA" id="AAPYVWK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006377">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904715">
        <property type="term" value="P:negative regulation of chaperone-mediated autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000335">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF471">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005150858" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="466" mass="52234" checksum="B1A61D3697D156A9" modified="2014-04-16" version="1">MFAKCSLLVATLCLLGCWGQYAPDLVTSLPGLSETPNFKHYSGYLQAGSGNYFHYWFVESQNNPSTDPLILWLNGGPGCSSMDGMLSENGPFHLNDDGTVYMNPYSWNKLANVLYLESPAGVGFSYSTSQNYQTSDPQVAADNYQALLDFFSKFPSFAGNDFYVFGESYGGIYAPTLTQKIASSSNSINLKGMGVGNGMTSYALNDQSLVYFGYYHGLFGDNLWSTLNQYCCSGDSCNFYSSSNSNCQDAVYQALYEIQDIGLNIYDLYSECYGGAGYSGLYAADTSNLFRNYNFRFQAPQQKQISHKLLLYSVEASIPGDPRCINGTAMYYWLNTPAVRAALHIPSFVQTWELCSHTVGSLYQRTYQDMTSFYQDLLQKNLQLLVYNGDVDMACNFIGAKWFVESLNQQLVSQYKPWFYNDQIAGYVEQYEKITFLTVKGSGHMVPQYKPGPALQMFQNFLSNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIE1</accession>
    <name>W5MIE1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01038179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIE1"/>
    <dbReference type="FunCoup" id="W5MIE1">
        <property type="interactions" value="827"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008150"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008160.1">
        <property type="protein sequence ID" value="ENSLOCP00000008150.1"/>
        <property type="gene ID" value="ENSLOCG00000006734.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234624"/>
    <dbReference type="InParanoid" id="W5MIE1"/>
    <dbReference type="OMA" id="EPNTHIM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006734">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000068">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000083">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000074">
        <property type="entry name" value="Desmoglein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009122">
        <property type="entry name" value="Desmosomal_cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF1">
        <property type="entry name" value="DESMOGLEIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01818">
        <property type="entry name" value="DESMOCADHERN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01820">
        <property type="entry name" value="DESMOCOLLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="78"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="190"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="308"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="733" mass="81977" checksum="BEF7AB918E73ACF9" modified="2014-04-16" version="1">VLYSIHGNGVDQDPLNVFIVNPYTGFIKVTDTLDREKIAMYNLTGIAKDTNGEILEKEIKLRIAVIDKNDCSPVFEEDKYAEVYELSETVILGTPVVRMTATDDDDPATDHTKIKYSIVSQSPPEPQIFSMNETTGMISVIKPYLDREQQSTYTLIVKGTDMDGKENGNTGTGTVHITVLDVNDHLPKLEKDEYSGSVDENTENVEVMRIKALDEDLEFSDNWMAEYDIVSGNEGGYFSFTTDTETNEGILMLNKALDYEVIQNLELSVAVRNKVVIHSRAAASFSSGTKTYPLKIHVKNQPEGPRFIPKVKAVSISEETKKVKLKSVITKYLAVNSDTGMTAERVRYAKAFDPDNWLSIDENTAEIKLNKLPDRESKFLVNGTYYAKVMVMTNDLPAKSATGTIALQVEDSNDHCPVLTSTSQVLCTESTAVFVTAEDEDADPNGPPFEFEIIPEGTKGTWIVEPLNDTTAILRTHDNLWPGSYEVAVEVKDQQGLSCPDKQVVKIDVCTCNEQKTCEATRKTTGAGLGFAAMGLLILGFLMLLCKTSKCYMITPKKRFKKFRPFDTKEHLIAYHTEGQGEDKILKLSVTILRCVFLTQEIPLLSAPLEIVPGRFYFGKEEWDFMSIWHDNIHVKTGNMCQEEMLKQIRSGTTYSIHEDSYVDIALSERYLKSRYITEPDTEPEALVFNFEGRESPVGSVGCCSFIEGDDDLEFLNDLGPKFKTLAEICTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKF7</accession>
    <name>W5MKF7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Src-like-adapter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Src-like-adapter protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018129">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635858.1">
        <property type="nucleotide sequence ID" value="XM_006635795.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKF7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008866"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008877.1">
        <property type="protein sequence ID" value="ENSLOCP00000008866.1"/>
        <property type="gene ID" value="ENSLOCG00000007319.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685406"/>
    <dbReference type="KEGG" id="loc:102685406"/>
    <dbReference type="eggNOG" id="ENOG502QPWY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159104"/>
    <dbReference type="HOGENOM" id="CLU_084503_1_0_1"/>
    <dbReference type="InParanoid" id="W5MKF7"/>
    <dbReference type="OMA" id="ETTNGFY"/>
    <dbReference type="OrthoDB" id="9924021at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007319">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005154">
        <property type="term" value="F:epidermal growth factor receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10344">
        <property type="entry name" value="SH2_SLAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000134">
        <property type="entry name" value="src-like-adapter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000039">
        <property type="entry name" value="src-like-adapter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035052">
        <property type="entry name" value="SLAP_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="28"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="90"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="248"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="286" mass="33010" checksum="22DB7FE2084AE839" modified="2014-04-16" version="1">MGNAMKTQTTETEIENRTAFIDQVLKAADNDMLVVLHDYPSPDISEPIFRTGEKLKVLSEEGCWWKVRSVVTGNENYIPNNHVAKVYHGWLFEGVARHKAEELLQLPSNRVGSFMIRESKTERGVYSLSVRHRTIKHYRIFRLPNNWYYISPRLTFQCLEDLVNHYSEMADGLCCILTSPCLTFSNNNLNLANQPPPVVMRRNNFDWNNVERSELIDQSAAPHRENRDSLISFGVRNSLASYLSLSSTGKTSQKNSRKKKSKSVYAVPDSEHNDFPIEEEYYEEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKJ5</accession>
    <name>W5MKJ5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007689">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKJ5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008904"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008915.1">
        <property type="protein sequence ID" value="ENSLOCP00000008904.1"/>
        <property type="gene ID" value="ENSLOCG00000007348.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZRA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159005"/>
    <dbReference type="HOGENOM" id="CLU_073233_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKJ5"/>
    <dbReference type="OMA" id="EEWTPNP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007348">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047012">
        <property type="entry name" value="ICAM_VCAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003987">
        <property type="entry name" value="ICAM_VCAM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771:SF9">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01472">
        <property type="entry name" value="ICAMVCAM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866398" description="Ig-like domain-containing protein" evidence="11">
        <location>
            <begin position="26"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="116"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="227"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="309" mass="34027" checksum="9DF8D880CB5AF072" modified="2014-04-16" version="1">MEFQSISLSCSFAQLLLLFLSDCGAVPECPLEISPQRAVVRYGDPVTANCTTTQTARGIGWEAPVGATGMKTGVQSVTWNVSSLTDWTVEPTCYGDFNTEPRECRKNLNISLYKSPDSVSLSSDGPVDQMVEGRQYQLQCEVQNIAPVQYLTVNWYRGQTLIHSQTFSHNTTRTPVNVSVRHLFTADRADNGQQFTCEAELNLGPEGPQPPPATRAKPLKVTVLYRPVITCPPSATVKEGQILVSSDLCTAVGNPPPDTQWLIEGQEIEPNDPLSRRDIGQFTFEAENEYGKVTQNVIITVESHKCCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5ML15</accession>
    <name>W5ML15_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-type lectin domain family 4 member E-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ML15"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009074"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009085.1">
        <property type="protein sequence ID" value="ENSLOCP00000009074.1"/>
        <property type="gene ID" value="ENSLOCG00000007477.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686993"/>
    <dbReference type="KEGG" id="loc:102686993"/>
    <dbReference type="eggNOG" id="KOG4297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="HOGENOM" id="CLU_049894_7_0_1"/>
    <dbReference type="InParanoid" id="W5ML15"/>
    <dbReference type="OMA" id="KLPWICA"/>
    <dbReference type="OrthoDB" id="538816at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007477">
        <property type="expression patterns" value="Expressed in heart"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF9">
        <property type="entry name" value="CD209 ANTIGEN-LIKE PROTEIN C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="160"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="287" mass="32969" checksum="1B066842583AB5E1" modified="2014-04-16" version="1">MESEGFYTDLVYPKENVYDTIDIGKTVHRNPESQQTGVVESDVRPRPYRLPTTCLGLMCAVLLTVIIALSVYYNVFSHSVSATDLLSDTLVQHTVNHSNLAAFNKEFKEGENSIGSKAIELNKILQAFSDSFPVILEYCPVNSETQMRRCSPCPKNWLQHNEKCYFFSSETMNWVNSRSYCSSMGVRLVIIGSVQEQAFIWKMVKKSKKDFWIGLRNVCKKRGWRWVNENRLQRYVYWKPGQPYNGEQRRSCVAAEAGVSTLNNWKNHECTDKLPWICAAEALRVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLE8</accession>
    <name>W5MLE8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBC1 domain family member 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006635905.1">
        <property type="nucleotide sequence ID" value="XM_006635842.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLE8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009207"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009218.1">
        <property type="protein sequence ID" value="ENSLOCP00000009207.1"/>
        <property type="gene ID" value="ENSLOCG00000007587.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695082"/>
    <dbReference type="KEGG" id="loc:102695082"/>
    <dbReference type="eggNOG" id="KOG2595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014944"/>
    <dbReference type="HOGENOM" id="CLU_039465_1_0_1"/>
    <dbReference type="InParanoid" id="W5MLE8"/>
    <dbReference type="OMA" id="CEREMPF"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007587">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000024">
        <property type="entry name" value="TBC1 domain family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1310:FF:000001">
        <property type="entry name" value="TBC1 domain family, member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF11">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="62"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="403" mass="45972" checksum="A89E38F30BAD3B73" modified="2014-04-16" version="1">MKKGKKHQQNTGSQVTGNGTVKKQGEHESKKKQKLVEIHRALNSDPVDIETLRRAAVSEGGLVADEIRRKVWPKLLSVNVYNLPPRPGKDVRESHKDYNQVLLDVRRSMRRFPKGMRAEQREVLQEQLTDIILDVLKRNPQLHYYQGYHDIVVTFLLVVGERMAAAMVDTLSNHHLRDFMDPTMDSTKHILNYLMPILEEVDPELHDFMQRAEVGTIFALSWLITWYGHVLSDFRHVLRLYDFFLASHPLMAIYFAAVIVLYREKEVKSCDCDMASVHHLLSQIPQNLPYEELISTAQELFTRYPPVVLAKKAALQPRESITISSFKDFQMATLQQRPDTVLRQQLRDRASSDLAVPEEVAPAGTSQLVKMAVWGISATLGAAALAVAQTALDWAPEFLLQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MMC1</accession>
    <name>W5MMC1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">NADPH oxidase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">CGD91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Cytochrome b(558) subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Cytochrome b-245 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Heme-binding membrane glycoprotein gp91phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Neutrophil cytochrome b 91 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">gp91-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">gp91-phox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">p22 phagocyte B-cytochrome</fullName>
        </alternativeName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="31">Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. NADPH oxidase complex assembly is impaired through interaction with NRROS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>NADPH + 2 O2 = 2 superoxide + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63180"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639192.1">
        <property type="nucleotide sequence ID" value="XM_006639129.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MMC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009530"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009541.1">
        <property type="protein sequence ID" value="ENSLOCP00000009530.1"/>
        <property type="gene ID" value="ENSLOCG00000007849.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692101"/>
    <dbReference type="KEGG" id="loc:102692101"/>
    <dbReference type="CTD" id="1536"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166110"/>
    <dbReference type="HOGENOM" id="CLU_005646_3_1_1"/>
    <dbReference type="InParanoid" id="W5MMC1"/>
    <dbReference type="OMA" id="FTFAKEH"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007849">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000030">
        <property type="entry name" value="cytochrome b-245 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000004">
        <property type="entry name" value="NADPH oxidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF60">
        <property type="entry name" value="CYTOCHROME B-245 HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="35">
        <location>
            <begin position="284"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00030106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042476"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00042961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00043221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00043223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00049908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00050020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00050046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="567" mass="65185" checksum="E9E3595054E034E8" modified="2014-04-16" version="1">MGNWVVNEGLSIFVILVWLGINAYLFVHFYIVYLSDRFFYTRVLLGHALSWARAPAACLNFNCMLILLPVCRNLLSFLRGSCQCFSRNVTRQLDGNITFHKLVAYMIAFHTAVHIIAHLFNFEWFMDAQLDNTTSLPYVLSQIGNGDNRSYLNPIRTNQTNPTEVAFTYLAGLSGVASTLALILMITSSMEVIRRSYFEVFWYTHHLFVVFYIGIVIHGIGRIVRGQTAESIKVHDPKQCISQYQTWGKNNTECPVPDFAGNPPMTWMWVVGPMFLYLCERLVRFWRSQQKVVVTKVVTHPSKTIELQLKKKGFKMEVGQYVFMKCPSISQLEWHPFTLTSAPEEDHFSVHIRIVGDWTQKLFEACGCDKKEFQEAWKLPKIAIDGPFGTASEDVFRYEVVMLVGAGIGVTPFASILKSVWYRYSHNNTNLVTKKIYFYWLCRETQAFEWFADLLQSLESQMLERGNADFLSYNIYLTSWNEAEATHFTVHHEEENDPITGLKQKTLYGRPNWDNEFKTIASNHPGTRVGLFLCGPESLAKSLGKNCNTHSDADPRGVHFIFNKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNG1</accession>
    <name>W5MNG1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nestin</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNH5</accession>
    <name>W5MNH5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 25">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="EMBL" id="AHAT01002715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNH5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009934"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009946.1">
        <property type="protein sequence ID" value="ENSLOCP00000009934.1"/>
        <property type="gene ID" value="ENSLOCG00000008182.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT06">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159053"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_2_1"/>
    <dbReference type="InParanoid" id="W5MNH5"/>
    <dbReference type="OMA" id="TIVGDHK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008182">
        <property type="expression patterns" value="Expressed in bone element and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:macrophage activation involved in immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:neutrophil activation involved in immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05116">
        <property type="entry name" value="PTKc_Syk"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2_C-SH2_Syk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09938">
        <property type="entry name" value="SH2_N-SH2_Zap70_Syk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.930.10">
        <property type="entry name" value="Syk Kinase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023420">
        <property type="entry name" value="Kinase_SYK/ZAP-70_inter-SH2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035838">
        <property type="entry name" value="SYK/ZAP-70_N_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012234">
        <property type="entry name" value="Tyr_kinase_non-rcpt_SYK/ZAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000604">
        <property type="entry name" value="TyrPK_SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 19" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="166"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="363"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="369"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="627" mass="72064" checksum="A5C88B522C4CBE65" modified="2014-04-16" version="1">HLVMADKVNELRFFYGNITREEAEDYLRQSGMSDGLYLLRQSRNYLGGFALSVAYSRQCYHYTIERELSGTYAIAGGKSHKTPSDVIDYHAQESDGLVCLLKKPCNRPRGMQPKVGPFEDLKEQLIKEYVQQTWNLRGAALEQAIISQRPQLEKMIATTAHEKMPWFHGCISREGSEPRLLQSPRINGKFLIRERDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPDGKKFDTLWQLVEHYSYKSDGLLRVLTEACPRPDFAAGSINIGPPRLPDHHPDTVYTIATNIYVECRIKNIRLRVISLLPSRGHAATAHQNTYEPSVARNKIKEAMPMDTSVYESPYADPEELKPKTVHLDRKQLYLEDGELGSGNFGKVMKGIYKMTKTEKPVAVKILKNDDNDPAVKEEMLREANVMQQLDNPYIVRMIGICDAENLMLVMELAELGPLNKYLQKHKHITVRNITELVHQVSMGMKYLEDNNFVHRDLAARNVLLVTQHYAKISDFGLSKALAAEENYYKAKTHGKWPVKWYAPECINFFKFSSKSDVWSFGVLMWEAYSYGQKPYKGMKGNEVLQMIERGDRMEAPAECPSEVYDIMRKCWTYKTDERPGFHAVEGRLRDYYYDISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQ47</accession>
    <name>W5MQ47_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP-binding protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5MQ47"/>
    <dbReference type="FunCoup" id="W5MQ47">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010506"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010521.1">
        <property type="protein sequence ID" value="ENSLOCP00000010506.1"/>
        <property type="gene ID" value="ENSLOCG00000008641.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2486">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001083"/>
    <dbReference type="HOGENOM" id="CLU_033732_5_0_1"/>
    <dbReference type="InParanoid" id="W5MQ47"/>
    <dbReference type="OMA" id="RMDHAPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008641">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000857">
        <property type="entry name" value="GTP-binding protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="8">
        <location>
            <begin position="109"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="329" mass="37106" checksum="79AF666A90DF27C7" modified="2014-04-16" version="1">MLLNRIIHLRTWTVLPKTISVIFSRQGAHSLSSFRQAAQMPEKKLQGLVFPFSELESHLSSRVSKTNFQLFQPSFEDIERAEALFSPSKKHKIDYFTSAVRMDHAPVITQPEVCFIGRSNVGKSSLIKALFSLVPEVEVRVSKTPGHTKKMNFFTVGKAFTLVDMPGYGHKAPKDFVEMVEAYLETRTNLKRTFLLIDGSVGLQEADRIAIGMCEEFGLPYVIVVTKIDKASRGELLRHIVEVEDTIKMHTLGCFPQPFLVSSTQFKGIYLLRCFIAHVTGNLHLKSDSQIKWATFQVPSRAFLFFQDSVPWALSGISLSGCAIKDDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQK3</accession>
    <name>W5MQK3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Fibroblast growth factor receptor</fullName>
            <ecNumber evidence="25">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24 38">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="25"/>
    <dbReference type="EMBL" id="AHAT01003998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069045132.1">
        <property type="nucleotide sequence ID" value="XM_069189031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQK3"/>
    <dbReference type="FunCoup" id="W5MQK3">
        <property type="interactions" value="385"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010662"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010677.1">
        <property type="protein sequence ID" value="ENSLOCP00000010662.1"/>
        <property type="gene ID" value="ENSLOCG00000008752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694148"/>
    <dbReference type="CTD" id="2263"/>
    <dbReference type="eggNOG" id="KOG0200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155447"/>
    <dbReference type="HOGENOM" id="CLU_000288_74_2_1"/>
    <dbReference type="InParanoid" id="W5MQK3"/>
    <dbReference type="OMA" id="PANCTSE"/>
    <dbReference type="OrthoDB" id="6019866at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008752">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05857">
        <property type="entry name" value="IgI_2_FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05101">
        <property type="entry name" value="PTKc_FGFR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000007">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000020">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000252">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000011">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016248">
        <property type="entry name" value="FGF_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF130">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000628">
        <property type="entry name" value="FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21 29" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="22" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 25 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 25 30" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 33" id="KW-0732">Signal</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="33">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866498" description="Fibroblast growth factor receptor" evidence="33">
        <location>
            <begin position="41"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="35">
        <location>
            <begin position="42"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="35">
        <location>
            <begin position="172"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="35">
        <location>
            <begin position="274"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="34">
        <location>
            <begin position="500"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="801"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="31">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="804"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="645"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="506"/>
            <end position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 30">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="584"/>
            <end position="586"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="29">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="81"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="197"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="296"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRNR" id="PIRNR000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="840" mass="93579" checksum="19722B43761655F0" modified="2014-04-16" version="1">MGSVSGWRSTGAWGISASRMSTWACLLYLALVSMATVSLARPPFNTEEETTLEPEEPPTKYQISKPTVCSVHPGDLLKLSCPLPEAGTITWTKDGSSLGSNNRTLIVEESLQIRDATPKDSGLYACSTVSPIGSDTLCFIVNVTDAISSGDDEDDTERSEDVPGDGEQMRAPYWTMPEKMEKRLHAVPAANTVKFRCAAGGNPRPHLRWLKNGRNFRQEDRMGGYKVRHQHWTLIMESVVPSDKGNYTCVVENQYGSINHTYTLDVVERSPHRPILQAGLPANTTVRVGEDARFVCKVYSDAQPHIQWLQHIEKNGSRYGPDGQPYVRVLKTAGVNTTDKEIEVLSLPNVTFEDAGEYTCLAGNSIGISYHTAWLTVLPADPEEPIFGPTSPDYVEIAIYCIGVFLIACMVVIVVVCRMKNTAKKPDFSSQPAVHKLSKQIPLRRQVTVSADSSSSMNSSTPLVRITTRLSSAVDAPMLAGVPEYELPEDPRWEFSRDKLTLGKPLGEGCFGQVVMAEALGIDKDKPKEALTVAVKMLKDDATEKDLSDLVSEMEMMKMIGRHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDIARVPDEQLTFKDLVSCTYQVARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDVHNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPGNCTNELYMMMKDCWHAISSQRPTFKQLVEDLDRILTLTTNEEYLDLCAPAEQYSPSFPDTRSSCSSGDDSVFSHDPLPDEPCLPKFQHMNGSVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQM1</accession>
    <name>W5MQM1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQM1"/>
    <dbReference type="FunCoup" id="W5MQM1">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010680"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010695.1">
        <property type="protein sequence ID" value="ENSLOCP00000010680.1"/>
        <property type="gene ID" value="ENSLOCG00000008775.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204328"/>
    <dbReference type="HOGENOM" id="CLU_049894_10_3_1"/>
    <dbReference type="InParanoid" id="W5MQM1"/>
    <dbReference type="OMA" id="AYICAIN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008775">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="98" mass="11140" checksum="5E72D8C2F78A3EF9" modified="2014-04-16" version="1">CIELGGNLASVHSEEEETFLLHLIVSKRATSTWLGGSDGEQEGKWLWSDGSKWAFTKWNAWEPNNSGTENCLLTYWDHGNWNDMNCNNQFAYICAINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRT3</accession>
    <name>W5MRT3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Collectin subfamily member 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRT3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011092"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011108.1">
        <property type="protein sequence ID" value="ENSLOCP00000011092.1"/>
        <property type="gene ID" value="ENSLOCG00000009099.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159374"/>
    <dbReference type="HOGENOM" id="CLU_049894_3_2_1"/>
    <dbReference type="InParanoid" id="W5MRT3"/>
    <dbReference type="OMA" id="FICEFLK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009099">
        <property type="expression patterns" value="Expressed in liver and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03591">
        <property type="entry name" value="CLECT_collectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051663">
        <property type="entry name" value="CLec_Tetranectin-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033990">
        <property type="entry name" value="Collectin_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 11 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799">
        <property type="entry name" value="TETRANECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046960979" evidence="9">
        <location>
            <begin position="29"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="10">
        <location>
            <begin position="147"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="275" mass="29992" checksum="DFC8805C53B887F5" modified="2025-10-08" version="2">MIRAMDNQKLTGNLLLLLLFQHFIFNYGTEVCSNTILPGMKGEQGGKGDEGVEGKMGKMGPPGLKGLPGEPGSRGDFGMMGKIGPVGAKGDKGVKGIEGPPGFKGKPGITCDCGRYRKVVGQMDINISKLKNSVKFVKNVILGVKETDEKVYLIVKEAKKYKEALINCRLRGGTLAMPKDEDTNSLIASYVSQAGLTRVFIGLHDSDKEGQFVYVDGTPLQNYTGWSPGEPNNAFVNENCVEMENTGFWNDVECDLTIYFVCEFWKKRRAAATVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSM8</accession>
    <name>W5MSM8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BEN domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <sequence length="415" mass="47610" checksum="56ABB3CD6FC38E96" modified="2014-04-16" version="1">MYAFVRFLEDDVCYALPVSSVKDFRPLHKTDFDNQKVYLVHRTEGNGTGQPGKAQILALAENIEEFEHSIMQKKMKIPKLSTNALDNSVENHFGEELLPLKHKKGLQAQEHGRSNSSSSKSLAAVVARLERNAVSSCMEGEEDYEDSLVEDSGAEEAVVPRVLYEELVHSYRQQEEEMRRLQQELERTRRQLVQQAKKLKEYGSLLTEVKELRDFNRRLQDVLLMRLGSEPMHDNGTQTIKTEVVEPIIEPQEVCREEANTSSSHSPSPRTVYTFNDGKVHLGGGIWVEEEKWHQLQRTQGDSKFTKNLAVMIWGTETLKNRSVTGVATKKKKDALPKPPLSPSKLKIVRECLYDRVSQETADSAEITQRLSKVNKYICEKIMDINKSIKNEERRESKLLIRQTVKMENFNYDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU74</accession>
    <name>W5MU74_LEPOC</name>
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        <submittedName>
            <fullName evidence="17">Spectrin beta, non-erythrocytic 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Actinin_actin-bd_CS"/>
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        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
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    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="16"/>
            <end position="119"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="3740"/>
            <end position="3765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="3893" mass="449098" checksum="F909198BF56AD924" modified="2014-04-16" version="1">EYGQGRIRELQEQRMAVQKKTYTNWINNVFSKNRTAVQDVYTELKSGIYLIRLLELISETKLPTPSQRDMRVHYLENNSIAIKFLKTKIKVDLIGPENIVDGDRTLILGLIWIIILRFQIATITLEEDEFGASAARRSAKEALLIWCQRRTAGYPSVDVRDFSGSWRDGLAFNALIHAHRPDLFDYSRLRQDQALQNLNHAFGVAERELGVSRLLDAEDVAVPQPDEKSIMTYVSLFYHYFSKMKQGQTGQKRIAKIVGLLKEIDDLKLQYERMVSELLKWIKTKVMELNDRLFPNSLHGVQRLVAVFKTYRTVEKPPKYQERGAIEALLFNLKTKLRANNQKAYMPPEGKTLGDVEKHWKLLEKAEHERERALQGELLKLERLEQLAQKFGRKASLREGYLADTVDLLRKQDFRAFSSLEEAEAASRRLEALNTDVQAREQRFRALSDMAAIIEKANYHSKVQITKRQEEIDRHWKHLLLQLKKYQESLRGAVQTLALFRDIEVVSQDLQELQVQASSADVGRQLHDVVALLQKQDLLDSQITSHGETLRSISRQAGAGRHSDPKQVQTRIQALNTQYHNLVSQSKSRSQALQEQLKLFEFFRDCEEVESWIYEKWQVVRTATLGRDLSQILLTIQKHKALEAEVQSHETLCTSVIKRGQNLCKKRHPSERDIQKWTEALQKQWQQLKDEVANRKTRLEAASIIKQYFADVNEADSWLRERHPLLTTEDCGKDESSADALLQRHLRLEKEVVAYASEVKRLGEQAKIAALQAPLTTAIDGHLNQRAEPQDEDKGAERGRPATRRAGRVTQVAPRMQEEAQAHIGQAKIRFKYRGEKVTLDKGEMVEVLNKPNKENWLVKDSRGHELLVPVFYITELQAKDSAHKMKIKQIKVKVQPVPPALKNGVLESPPQKVSRPRRSRSMRRGTTEIQSSSVPDPHFQKDTIASTQSVLDSDFHSLCRMAQSRRKALEEMIRLHRFYSACHGFESWMDDKENVLNTFQSNSENVEAMQVKYENFLTELASGKGRLDGLTQLADELVKSRHSKQGEILSRQSQANRRWERMQKLKDEKARELLGKADVKSFLQSCQDARVLLEEKRTQLEGPDMGTSSSALKTEQRRHSQVEREIQALERKIEYLKNVAQMKKDSSPAESEAIMEEVRSLERLLGRVKEGARQRHRKLDEGVRRQRFLQESRDLLLWAEAMREQLDGEESASDVASAQRLLKENQDLRKELDSQRERMKAMEELGNSLAHSSPANASEVRQTQGKLAREWAELDKLWVKRNRRLEEGVELQTFNREADRVEAALSSHEARLRVQDLGDSVDSVHSLLGRQEELESLLNTLDQRIGLLQDRGKDLTRKGHFAAKQIQQRAQAIQQKRDELGERSKQRKTMLLASKNFQEFNRDASELLMWMDEKFKIAEDESYRDPTNVLRKLKRHEAAEREMLANQVRVDTLKEKGQGLLAQSPYQRQAIQDRTEEVCSRWEELQRKMTDRGDKLRQAGQQEQLMELLQDARVKIEALERMLRDAGKGHDLRSSRQLLKEHRELEKEAQELADKMNAIVSRARSMATDHFDSDRILQETEKYLTWFESLQGPLAHRRTQLETSVALFEYYHDVDFELNWISERLPLAISTNYGKSLASALSLLQKHKDLQAEVNAHRQHVSRVLKKGGVMAESRQFRAEDVLERSRELKLEWGKLEKACEDRASCLHWAVTHQQIVLDTAELEACVSETLPLVSSDDYGKNEQATLSLIKKHKVVGGEVEALCEQVGELRHRAERASQERGQKGFDEVDWPQDRLQSQLGKLQHLAAIRSQRLEEAFRFHEFSRESGELEDWIAQQRQAASSEDYGNDYDHVQHLQGKFDVLQRQLEVGTERVHSCQGLADSLILHGHPQARQVQELQDRLRRSWEELLELARVRGEKLQDAEEYHRFYRDLTDALNHIEEKYKSIPDDIAKDLRGVLAQLRKHEALEHELAGNEQQLQELLDSVEPILERCSQEQASLLQDRQQAVVERWEKLRARVEQRREDLEQASRRYRFLNTVQDYFLWCAQVLSYMKAEVSIRDVSTSNMQLMQHQQLWAEIQAREETFHQAVTMGQELLQEEIPAPKEVRDKLGALHAERAKLQEHWNCKKLWLEATFLEQVFYRDIAHMEKLMNTQEIHLKSSDLGASVDETERLIKRHEAFEKLLSSQEEKVKSLQEQAERLKKEDVKKENAAHIQHKLKAVLERRSRIKELSARRREELSTARLFSVFNRDLAEAEGWISERMQKMQDDSKKDLSDLQVKMKLLQKHQAFEAEILAHEEIITSVLKTGEELVTLRHPKSSEVRRGARVLNDHWEALKRAVAARGKVLEDNRDFLEFLQKVDQVEAWIRQKEVMINVGDVGEDYEHGLQLLKKLNEFRGTGSGEVTVDDAHIKAINALAARLERQHKEEVTTVRQRRQQMNDRWSSFHGNLSRYRKKLEGALEVHALIRELEELRERSGEKSLLMQGLDYGQDVPSVENLIRRHEETEREIGIIQEKAAALEKEAKTKVKTQPVMAEKLNRKQKEMRDAWLKLEKEAKVRKEKLQASLQLQMFKADQRPLLDWVLKVTTQMGESGLPKNKAEAEQFIAEHQNTKAEIDARGDRFDSVKNLGQGLVRSGHYASSEIRQALSKLDEARTGLNRVWQDRSQKLDQALELQVFYGCVEQCESWLSNKEAFLANEDLGDSLSGVETLQRKHALFEKAVEAQLENVETVERFARQLVQKRHPHSGDIQSKSKAVLLRKEKLMENSAIRRRRLEDSLQLQKFLGSSYEVCSWLSEKNTVALDESWRDPTNLQAKLQKHQSFEAEILANHNRVEALTKEGERMLGSGHYAHDQISPRLQDLQGSWEQLLSNCKEKRSRLQEAYEALQFQRSLEDVEDWLASVESELLNTDNGCDLPAVSRLLKALQGLEEGVEGHVERIQGLVDAAKDFRAKGNFLAEEIQRRVGETVYRYNSLAEPLQARRETLEAWQLLFQLYRDMEEELVWVRDKLPSAYSQDWGSSLHGTQTLLKKHQTLQEEIASRDPLVRAVLEAGNTMVKARHFASKEIRERLAELKEAVESLRREAEHRGRGLQEALEIQVFLAELSELGSWMEERRPALECSDYGKSEESTQALLRKLDSVDLDLESQRPKLESLQETGAKLERYGHPNSHLVNESLQPVWDQYQSLQQLSAERRAALEEHCRVYEFEREARELASWLSAQRSTAESEEYGQDLEDVEVLQKKFEDFSSEVSTLGHSRVRSVLQLGQKVRSPECQEREAELCKLWEELLQTIEARAENLRSAREVHQFDHDVDELKGWMSEKEAGLSAEDHGHDLLGVQALLRQHDGLERDLAAIGEEVSRTREKGAVLGHRYPQVRDSLEERLEEVEEAWQSLQRKASVRRERLGQAETAQRYFIDWRELVAWLKESLSLVQGEGLSGEGGDVEQHLKRHEEHHREIDRQLDKSQAVKEEGRRLIESGNFMSEEVEEKLAELEELEERLGQGRRERQRLYDEELEVEQLRRGLEQAERWLNAHEAALSADVYGVSWGPGRSGAGQRENRRHNYTSYMTLELKLKSLELKTTFTAHKQPLIVFCFREDSVPDVLELMKRQEDLETMLTAQEERFKQLEEKRTKLENVTNNVKEQRLQQLQGQEGEGSKPTRVTSLRRKLSDRRPAGPTKSPTPKLPSLPPPRSQPPRSSHTDTVVSPVRRSLRSPAEATPPSPHPRPLGLHAVKDPSPTSPQKPPLPPKPAVPQSPVSPPANPGTPTSPVHVLARSFSSPPPAPGLPSHPLEGPLEVKQKLLADGNKTSALPWGFFYTVLDTDTLILYRDLEEATTESSSLPPICTVGAVCDVIPNHKKKDYVFRLTLSDSSQYLFSAPSQEVLQCWVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUG8</accession>
    <name>W5MUG8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01010425">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012027"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012048.1">
        <property type="protein sequence ID" value="ENSLOCP00000012027.1"/>
        <property type="gene ID" value="ENSLOCG00000009841.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158377"/>
    <dbReference type="HOGENOM" id="CLU_255249_0_0_1"/>
    <dbReference type="InParanoid" id="W5MUG8"/>
    <dbReference type="OMA" id="KMSWPPE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009841">
        <property type="expression patterns" value="Expressed in mesonephros and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="291"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="205"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="681"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="800"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="865"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="836"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="821"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="865"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="877"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="898"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="918"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="945"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1386" mass="154435" checksum="3C5BE3A5299FCEAC" modified="2014-04-16" version="1">MEMKSSLRRAQSLKHISTESQQSWRDAALREKGKSVSQLVAQYQTSLDLKNADSEEQKQKILARDDPPDTKFEALLKTFESRQKPKKSAHYDNGNLLRSKSMEHLPRQGETSISSLLAIFESTSPLKETWRSPQVKQGLTADKSVFVPSKRERPVTRIWQKVVTSAEVKEIESIPTREEQAVNKNVTHGWPDRFLRGEMIGGLSPPLAKTMGQAQEKRLPRLDHQEAPSAETQTREKSLNSVKERSALYLPKVTAVDLPGSPIKPDNLAQQTVIITGNSPKTRKFQSPIKEMCSSCLKPVYPMERMVAEKSVLHNNCFCCKHCHKKLSLQNYAALCGEFYCMFHYQQLFREKGNYDEGFGRRQHKDQWLQKSSKDQKDDEKIIHKAKQESLTSHGSLEPLTIGSRNPLLPSRNQEEKNKSSSEPINKLRVSWPPPKQNLGTTHFAQIEKMTFKEPITEKPTTELNQSKSVVRHSLQGKDRYIPLSQNPEVNSRVFLSSIEKDTYKEKGCVGSSSTLCMKQDKENNVVNGKLKAPLETTDKTQSKESHPWKVSTKITLFQENTKAKVKHLSSAYKVTEVSPKSSLITKPSSELQDSFTPASKGSHQSVFNEQPRIPENTDQYDNFTHSPSKVTKMTYNAEGNLADDNSKANASKNNLAIGQISLNVSKELESVPKGEVQLFSEPGMKKEHSRSHPRASSPDTKKELSAEVAESVLKNENELHYINKTPQEENIATNAKDDISSAIKLYSQSDLTSNTRAECTIENHTNQKLSNTDEGDFETCSVQVNEKSLYKENSLVNVETQNKANSQEPAEKYSSLSNTERLDSESKISSEDTKLNIKEVIVKNAEEKVDETLNQCLNQDKKVEIAENQPKKAEKPNSQGYVNKQNGKTHTRKDSWSKVSGQGKSPLSMLFSSGSKDNTDKKEIKKPDAKPRSILGRLLQSTSEKGKELKKEKESKSDTLEKDSGEDGDKIPQEQKDENVLTTSGKEEGDEENILNSKQLNETNKINSTNKELMETPKGDPHITEIINPYGSPQDSLYLIPITMNDTERTLICSDKNESTLSSFSESNLKQTDEMSQDLSTDSINPFSSPEPCLTQKNTVNGSTNKLTNHIQISQQRITNLVEDIPTEIINPFCPPESQEEIAINKSENILFDSENCIASCNQTVASKNPFDSVNLNDPMNPDKDQLDILGISEPTISSPDPLLVSEGEMVNVMVSSVETQQSDEISDLLNLNLSSQVIVNEKINEDNLDTESIPQAQTTNVFDNDFSKLDAFSAPIPSTFLDVFGTGDFSAAVDSSTVSSLQQNSGNLDDLFGLGQTTVSSGSVPTTQGNIFPDDIVGFESLLPKPPATASSDIFLDPVSLDSNITATSANKIADNKWFDDFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUJ5</accession>
    <name>W5MUJ5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C1q and TNF related 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006629590.2"/>
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    <dbReference type="RefSeq" id="XP_015200272.1">
        <property type="nucleotide sequence ID" value="XM_015344786.1"/>
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    <dbReference type="AlphaFoldDB" id="W5MUJ5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012075.1">
        <property type="protein sequence ID" value="ENSLOCP00000012054.1"/>
        <property type="gene ID" value="ENSLOCG00000009865.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693607"/>
    <dbReference type="KEGG" id="loc:102693607"/>
    <dbReference type="GeneTree" id="ENSGT00940000159588"/>
    <dbReference type="HOGENOM" id="CLU_001074_0_3_1"/>
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        <property type="component" value="Linkage group LG4"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Complement C1q B chain"/>
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        <property type="entry name" value="C1q_dom"/>
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        <property type="entry name" value="Collagen"/>
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        <property type="entry name" value="Collagen/C1q_domain"/>
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        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
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        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
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        <property type="entry name" value="COMPLEMNTC1Q"/>
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        <property type="entry name" value="C1Q"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866802" evidence="7">
        <location>
            <begin position="17"/>
            <end position="286"/>
        </location>
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        <location>
            <begin position="141"/>
            <end position="277"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="129"/>
        </location>
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        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
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            <begin position="61"/>
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        <location>
            <begin position="102"/>
            <end position="111"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="286" mass="30195" checksum="2C462431EBDABAB6" modified="2014-04-16" version="1">MLVLIGIISVFNCISGQTLESKWKGNPPRYICSVPGLPGPPGHPGSNGAAGPHGRIGLPGRDGRDGRKGEKGEKGDPGLRGKAGPAGKIGGRGDRGPVGKRGPNGTGGEKGFVGPAGPPGEKGDKGNRGIPGKPGICKCGSLVPKSAFSVGITTSYPVELTPIKFNKVLFNEGEHYNPNTGKFICAYPGIYYFSYDITLANKHLAIGLVQNGQYRIKTFDANTGNHDVASGSTVMYLNPEDEVWLEIFFTDQNGLFSDPSWADSLFSGFLLYADTNYLDALSEDYA</sequence>
</entry>
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    <name>W5MUT0_LEPOC</name>
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        <submittedName>
            <fullName evidence="4">GIPC PDZ domain containing family, member 1</fullName>
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            <taxon>Actinopterygii</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GIPC family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021303">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000012160.1">
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        <property type="gene ID" value="ENSLOCG00000009925.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690130"/>
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    <dbReference type="eggNOG" id="KOG3938">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003420"/>
    <dbReference type="HOGENOM" id="CLU_044527_1_0_1"/>
    <dbReference type="InParanoid" id="W5MUT0"/>
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        <property type="component" value="Linkage group LG6"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <end position="199"/>
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        <location>
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            <end position="243"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009011"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="36204" checksum="CFFB7C13CDF8E1E9" modified="2014-04-16" version="1">MPLGLGRRKKASPLVENEEAEPIRAGMNVPGLEGSAESMHSFQPGLPPPPPSLRPRLVFHTQLAHGSPTGRIEGFSNVRELYSKIGEAFGIPAAEVMFCTLNTHKVDMDKLLGGQIGLEDFIFAHIQGQRKEVEVFKSEDALGLTITDNGAGYAFIKRIREGSIIDRIQVINVGDMIESINGQGLIGCRHYEVAKMLKELPKGQNFTLKLVEPMKAFDMISQRSGGGRSSSASQLGTGRGTLRLRSKGPATVEDLPSAFEEKAIEKVDDLLESYMGIRDCELAATMVELGKDKRNPDEFAEALDETLGDFAFPDEFVFDVWGAIGDAKVGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVI7</accession>
    <name>W5MVI7_LEPOC</name>
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        <submittedName>
            <fullName evidence="9">Relaxin-3-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Holostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013012">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015337181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVI7"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000012417.1">
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        <property type="gene ID" value="ENSLOCG00000010131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687267"/>
    <dbReference type="KEGG" id="loc:102687267"/>
    <dbReference type="CTD" id="6013"/>
    <dbReference type="eggNOG" id="ENOG502S5T3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154396"/>
    <dbReference type="HOGENOM" id="CLU_120043_0_0_1"/>
    <dbReference type="InParanoid" id="W5MVI7"/>
    <dbReference type="OMA" id="SQGCTKN"/>
    <dbReference type="OrthoDB" id="8784777at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010131">
        <property type="expression patterns" value="Expressed in liver and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04365">
        <property type="entry name" value="IlGF_relaxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051777">
        <property type="entry name" value="Insulin-like_neuro_ligands"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022353">
        <property type="entry name" value="Insulin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968">
        <property type="entry name" value="ILGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968:SF4">
        <property type="entry name" value="RELAXIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00262">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868831" evidence="7">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Insulin-like" evidence="8">
        <location>
            <begin position="35"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="189" mass="20818" checksum="789D0B87F663C766" modified="2014-04-16" version="1">MLKLLSVFLMCVGSMCLGARAQGVSKGGLTREYGVKLCGREFIRAVIFTCGGSRWKRLSEDSDSPLGIEKNLPLGSAFHTAPEGEDSLSRLQSILERPPLPITQLSLKDLVSLYGVYSDYESVPDDLGELLQQVSELRQQQKLQTTEKVGNSPHIPWLKSPRRKRNFSVGLAGMCCNQGCTKNDIGRLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVZ3</accession>
    <name>W5MVZ3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01034649">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015214855.1">
        <property type="nucleotide sequence ID" value="XM_015359369.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVZ3"/>
    <dbReference type="FunCoup" id="W5MVZ3">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012552"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012575.1">
        <property type="protein sequence ID" value="ENSLOCP00000012552.1"/>
        <property type="gene ID" value="ENSLOCG00000010256.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689278"/>
    <dbReference type="CTD" id="65061"/>
    <dbReference type="eggNOG" id="KOG0594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159606"/>
    <dbReference type="InParanoid" id="W5MVZ3"/>
    <dbReference type="OMA" id="EMFQGEP"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010256">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000131">
        <property type="entry name" value="cyclin-dependent kinase 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF159">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="88"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="422" mass="47978" checksum="D12C1AAC9FED27BD" modified="2014-04-16" version="1">MQNLRHAATEAFNRLGLKQRHLGYEELDDVGDSAFPSGPIQPQWFHTLQVRRLRVQRTRSNSDPLGGKSFEQEFQWRTGLKFGTATSYLNLEKLGEGSYATVYKGISRINGHLVALKVIRMKTEEGVPFTAIREASLLKGLKHSNIVLLHDIIHTRESLTFVFEYVQTDLGQYMVQHPGGLHSYNVKLFLFQLLRGLSYIHRQRILHRDLKPQNLLISYLGELKLADFGLARSKSVPSQSYSSEVVTLWYRPPDVLLGATDYSTGLDIWGAGCIFIEMLQGSPAFPGVADIFEQLQKIWAVLGVPTEETWPGVSELPNYKPEWFLPCEPRMFRNVWKRLGQLPYKAEDLAQKLLHSVPQDRLPAQEALQHEYFGSLSPALLQLPDTVSIFKVPGVRLETEIRDIFSPGRKSRPALAPLTKCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYJ6</accession>
    <name>W5MYJ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631944.1">
        <property type="nucleotide sequence ID" value="XM_006631881.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYJ6"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013455.1"/>
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    <dbReference type="KEGG" id="loc:102695921"/>
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    <dbReference type="eggNOG" id="KOG0074">
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        <property type="component" value="Linkage group LG6"/>
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        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
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        <property type="term" value="C:microtubule cytoskeleton"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <position position="75"/>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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            <dbReference type="ARBA" id="ARBA00004255"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00010290"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022927"/>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
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        <source>
            <dbReference type="RuleBase" id="RU003925"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="187" mass="20880" checksum="D1A241BFE5D1308F" modified="2014-04-16" version="1">MGEVQKGLFSVIQKLKGSTEQELRIVLLGLDNAGKTTLLKKLASEDVNTITPTQGFNIKSVASNGMKLNVWDIGGQRKIRPFWKKYLENTDLLIYVIDSADKKRFEETGQELSELIEEENLKGVPVLIFANKQDLVSAAPASEIAEGLNLHTYRDREWQIQACSALSGEGVQDGMNWICNNIVNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZ54</accession>
    <name>W5MZ54_LEPOC</name>
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        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Host endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Virion membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01032055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZ54"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013663"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013663.1"/>
        <property type="gene ID" value="ENSLOCG00000011124.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064449"/>
    <dbReference type="HOGENOM" id="CLU_075712_0_0_1"/>
    <dbReference type="InParanoid" id="W5MZ54"/>
    <dbReference type="OMA" id="CTFISAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011124">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF81">
        <property type="entry name" value="ERVV2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1032">Host cell membrane</keyword>
    <keyword evidence="7" id="KW-1043">Host membrane</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004531"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004563"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022511"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022581"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022870"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="243" mass="27430" checksum="C45A6EB7DD3917C0" modified="2025-10-08" version="2">MMDWYWQCGAKVNDSLHSQWRGVCALVTLHHSILIVPVNSYLLNSMKPLQRESRVKRALSWADRYGPDISKIPEDNRLFTAAEMIFSSGLSIQAVANARWLQITRYEMYLFINITLEGLDAIKQELRGVRLMALQNRFVLDLQNAASGGVCALIGDSCCTFISANDDDYGNLTLAIQHLKELRDKADKERGIKDTPAFLSWLESLFGLVTPIIVAVILLFLFLCCCTTCIIPMLLNMVMNMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZJ8</accession>
    <name>W5MZJ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">ArfGAP with SH3 domain, ankyrin repeat and PH domain 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017658">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634377.1">
        <property type="nucleotide sequence ID" value="XM_006634314.3"/>
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        <property type="nucleotide sequence ID" value="XM_069191060.1"/>
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        <property type="protein sequence ID" value="ENSLOCP00000013807.1"/>
        <property type="gene ID" value="ENSLOCG00000011231.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG9"/>
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    <dbReference type="Bgee" id="ENSLOCG00000011231">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ArfGap_ASAP1"/>
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        <property type="entry name" value="SH3 Domains"/>
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    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
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    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
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        <property type="entry name" value="ArfGAP_dom_sf"/>
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    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
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    <dbReference type="InterPro" id="IPR037928">
        <property type="entry name" value="ASAP1_BAR"/>
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        <property type="entry name" value="ASAP1_SH3"/>
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    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
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    <dbReference type="InterPro" id="IPR001849">
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF2">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BAR/IMD domain-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="324"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="20">
        <location>
            <begin position="439"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="600"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="1069"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="716"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="776"/>
            <end position="1072"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="735"/>
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        </location>
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        <location>
            <begin position="808"/>
            <end position="821"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="858"/>
            <end position="872"/>
        </location>
    </feature>
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            <begin position="879"/>
            <end position="889"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="905"/>
            <end position="915"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="916"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="936"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
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        <source>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1131" mass="125569" checksum="B41B9635EEF3A757" modified="2014-04-16" version="1">MRSSSSRLSSFSSKDSLWNRMPDQISVSEFLSETTEDYNSPTTSSFTTRLQSCRNTVNVLEEALDQDRTALQKVKKSVKAIYNSGQEHVQNEENYAQALDKFGSNFISRDNPDLGTAFVKFSTLTKELSALLKNLLQSLSHNVIFTLDSLLKGDLKGVKGDIKKPFDKAWKDYEAKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSKQGGYSMHQLQGNKEFGSEKKGYLLKKSDGLRKVWQRRKCSVKNGILTISHATSNRQPVKLNLLTCQVKPSGEDKKCFDLISHNRTYHFQAEDEQEFVIWISVLTNSKEEALNMAFRGEQSTGENSLEDLTKAIIDDVLRMPGNEICCDCGAPDPKWLSTNLGILTCIECSGIHREMGVHISRIQSMELDKLGTSELLLAKNVGNSSFNDIMEGNLPFPSPKPTPSSDMTARKEFINAKYVDHKFARKTCTSAAAKMSELFEAVKSRDLLALIQVYAEGVELMEPLPETGQEPGETALHFSVRTADQTSLHLVDFLVQNSGNLDKQTEKGNTALHYCCVYEKHECLKLLLRGKPSTDITNQNGETALDIARRLRNSQCEEALVQAAAGKFNPHVHVEYEWNLRLEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSLSPQDKLALPGFGSQRDKQRLSYGAFTNQIYSASTDSPTSPGIDAPPLPPRNAGKAPSGPPSTLPLGSQPPSGSSTLCKKRPPPPPPGHKRTLSDPPSPLPHGPQNKGGIPWGNDSTPPSVNKASSGNKFEGILQQQSTSTTKSTLGPRVLPKLPQKVALRKIDTIHHPSIDKSNQQPDVFQKSPQAAEIPPKVPLPEKPQPGDLPPKPQVGDLPPKPGELPPKPQLSDLPPKPLLGDLPPKPQVKDLPPKPQLSEVPPKPPGTDTPQKSLPGESAPRPPPSDSPGSNQLGEAAPRQLSEDTNGTPPAVMEMPVPLPRKINTGKNKARRVKTIYDCQADNDDELTFVEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPMSFVHILSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZL0</accession>
    <name>W5MZL0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mab-21-like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034986">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639436.1">
        <property type="nucleotide sequence ID" value="XM_006639373.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZL0"/>
    <dbReference type="FunCoup" id="W5MZL0">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013819"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013848.1">
        <property type="protein sequence ID" value="ENSLOCP00000013819.1"/>
        <property type="gene ID" value="ENSLOCG00000011250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689695"/>
    <dbReference type="KEGG" id="loc:102689695"/>
    <dbReference type="CTD" id="126868"/>
    <dbReference type="eggNOG" id="KOG3963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="HOGENOM" id="CLU_045315_1_0_1"/>
    <dbReference type="InParanoid" id="W5MZL0"/>
    <dbReference type="OMA" id="WSKKARW"/>
    <dbReference type="OrthoDB" id="5947963at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011250">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF30">
        <property type="entry name" value="PROTEIN MAB-21-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="2">
        <location>
            <begin position="83"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="3">
        <location>
            <begin position="280"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="44904" checksum="3411B297697E9EF1" modified="2014-04-16" version="1">MTGFTEEDLDNFLQNQVDLRHRQVSKTVDEVQQIIKDLTSEVSTKDGRFQSISNSGVHNDNIKDQPALMAKWAALLRGKCAFNPAIQVLTPALFLISVPVRGLCGYKERRARQWRYYTLSGSRLLSPVREPEKLHQWLELENFCSPSQEWHDSRVAIEGDIVPAKVVNLFKELLEAAIQSCNLASKVTILETVGAMIRVAVETSEAQVEAELIPTVELMNCWPKKARWPRFLKRWPSKEKARCIKSFGFNLMASSNYHWLLSFSRAEQVLMSSIDEDGGCRRKCYRVVRQLKEDVWCPGSKPVITAYHLQTLLFWSCEKYPCTKDWRNFKQSVLRLVKKLQKCVSQRYLRHYFVRGYNLLKYTNTNELDIMSKKISDFLENPGLYMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N118</accession>
    <name>W5N118_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">COMM domain containing 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N118"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014327"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014356.1">
        <property type="protein sequence ID" value="ENSLOCP00000014327.1"/>
        <property type="gene ID" value="ENSLOCG00000011660.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688094"/>
    <dbReference type="KEGG" id="loc:102688094"/>
    <dbReference type="CTD" id="54951"/>
    <dbReference type="eggNOG" id="ENOG502S00F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018121"/>
    <dbReference type="HOGENOM" id="CLU_116006_0_0_1"/>
    <dbReference type="InParanoid" id="W5N118"/>
    <dbReference type="OMA" id="KLCHRVV"/>
    <dbReference type="OrthoDB" id="17646at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011660">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04756">
        <property type="entry name" value="Commd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047235">
        <property type="entry name" value="COMMD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055184">
        <property type="entry name" value="COMMD8_HN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22838">
        <property type="entry name" value="COMMD8_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="109"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="176" mass="20202" checksum="DA900DD2803E644F" modified="2014-04-16" version="1">MIKLLEKLPRTECPKFLHRVIDGVCGRDPPRLHDYSSVWSLREWLELVDTLSSIFRTAIGKKMSDEEVQQSINWLDIGYQEAILTCLKGRKEEIRQALVEKTNAISSTQLQDFDWQIKLALSSDKISSLQIPLLNLDLDTKENGVLKPLSIEMNREELQNLINSLESANKVVLQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N122</accession>
    <name>W5N122_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HEAT repeat containing 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019089">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014360.1">
        <property type="protein sequence ID" value="ENSLOCP00000014331.1"/>
        <property type="gene ID" value="ENSLOCG00000011659.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPUU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013207"/>
    <dbReference type="HOGENOM" id="CLU_012662_0_0_1"/>
    <dbReference type="InParanoid" id="W5N122"/>
    <dbReference type="OMA" id="YATQSHN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011659">
        <property type="expression patterns" value="Expressed in testis"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF20">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="908" mass="102654" checksum="FE3B7092A29B7197" modified="2025-10-08" version="2">MKHLRQERLYLQFLHRISSGLTFTQDVVKERGPDTLSYSQMDFSRLFDASGLLTTTAKLKNHTSHKRATPRQLPVCSSGCAEELRPKNTYTISTLPPELPSLFPTELPPSLHSVSELSTVQRTDSCLLQSNQSEALNVSKKVKGKRKANVSGSLKKTVSDDCISSSSKWDEFVLKKLTKTTAQWIVSQQMPGQSANKARLQDLLKQHYGSASATDLVCDEPMNMEDFRRYHDLPKEEPKEQIVDGTHPETPLPLYYRVPGFCLLPTRPDEPGGNNWTADNVKVRHLEPPQPPRLQDRLNPRAGRYVYHTDNSFEQELYSGKISRQVHQHDGRQRDRIIMDNNSEYQKHLQDRFPRGPEEWTFRYSASQNKTSILKPEKGARRWVSLPSPADYAMERGLQPPDYSGQDTILPGTLRKQPQSCPPQERHALRYMVEEWRKAWKLMTRWQNVTIEGLKRNLSDLHYHVRIIAIATCASGAVNRPSMGQESTATAPFWRPRDVQVVPEELQPLLLSALEDPSDRVRLAAALCQYVMGTANRQARNILHSVLQQGVGADSWAAAQCLALEGDTSIAVIQRLLSQLYEGEAHSDREQATVLLASLSKSTTLVRSLLAEKLNSGNWRDRAVSCNTIAGLSGPINKDIANKLVFLMWNDWNRNVRLAAARALGKMELGKEVHNELRKKLEEAPPSWRVEALSLLAQLEIMTAKLLPAFLCCLSDDYAAVRKQACQTAAALGIKDEMVLNHLIHLMQTDPLWEVRVRAISALGKIGCLTPAVQEHLLWALHHEEEPRVRIATCDAIKALCVSGPELQHFLQERLILEANPEVQRKIADVLRTYGCSLNNDKDTICKIKEQVDKLCTKGVITQKVLMLEELEETHMQQKRHLDLASDPGRQFTHTDMARLLEDSYTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1F0</accession>
    <name>W5N1F0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Elongation of very long chain fatty acids protein 6</fullName>
            <ecNumber evidence="19">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">3-keto acyl-CoA synthase ELOVL6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ELOVL fatty acid elongase 6</fullName>
            <shortName evidence="19">ELOVL FA elongase 6</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-ketoacyl-CoA synthase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-oxoacyl-CoA synthase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ELOVL6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-hexadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-octadecenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:76555"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(9Z)-octadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-eicosenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74011"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z,12Z)-octadecadienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z)-3-oxoicosa-11,14-dienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36503"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74012"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36504"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(9Z,12Z,15Z)-octadecatrienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z,17Z)-3-oxoeicosatrienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74034"/>
            <dbReference type="ChEBI" id="CHEBI:74054"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dodecanoyl-CoA + malonyl-CoA + H(+) = 3-oxotetradecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:60140"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:60141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hexadecanoyl-CoA + malonyl-CoA + H(+) = 3-oxooctadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71407"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:35316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetradecanoyl-CoA + malonyl-CoA + H(+) = 3-oxohexadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39168"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="19">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Endoplasmic reticulum membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="19">N-Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ELO family. ELOVL6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="19"/>
    <dbReference type="EMBL" id="AHAT01017870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017871">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017872">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629893.1">
        <property type="nucleotide sequence ID" value="XM_006629830.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200775.1">
        <property type="nucleotide sequence ID" value="XM_015345289.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1F0"/>
    <dbReference type="FunCoup" id="W5N1F0">
        <property type="interactions" value="785"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014459"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014488.1">
        <property type="protein sequence ID" value="ENSLOCP00000014459.1"/>
        <property type="gene ID" value="ENSLOCG00000011759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689523"/>
    <dbReference type="KEGG" id="loc:102689523"/>
    <dbReference type="CTD" id="79071"/>
    <dbReference type="eggNOG" id="KOG3072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244965"/>
    <dbReference type="HOGENOM" id="CLU_048483_1_1_1"/>
    <dbReference type="InParanoid" id="W5N1F0"/>
    <dbReference type="OMA" id="PISWVPI"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011759">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03206">
        <property type="entry name" value="VLCF_elongase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033675">
        <property type="entry name" value="ELOVL6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF125">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 19 20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 19 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 19 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 19 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="31331" checksum="9A8A84B65F65BB48" modified="2014-04-16" version="1">MNMSVLALQEYEFERQFNENEAIQWMQENWKKSFLFSALYAAFIFGGRHLMKQREKFELRKPLVLWSLSLAVFSIFGAVRTGGYMVYILMAKGLKQSVCDQSFYNGPVSKFWAYAFVLSKAPELGDTIFIILRKQKLIFLHWYHHITVLLYSWYSYKDMVAGGGWFMTMNYLVHAVMYSYYALRAAGFRVSRKFAMFITLTQITQMVVGCVVNYLVFSWMQQGQCPSHVQNIVWSSLMYLSYFVLFCQFFFEAYIHKSKKDMSKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2N7</accession>
    <name>W5N2N7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Synthesis of cytochrome C oxidase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000316">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2N7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014896"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014925.1">
        <property type="protein sequence ID" value="ENSLOCP00000014896.1"/>
        <property type="gene ID" value="ENSLOCG00000012104.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2792">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004323"/>
    <dbReference type="HOGENOM" id="CLU_878629_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2N7"/>
    <dbReference type="OMA" id="TTHTREH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012104">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF4">
        <property type="entry name" value="PROTEIN SCO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
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        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="36269" checksum="028ABE9DB6C0A4ED" modified="2014-04-16" version="1">AAVGLENKHLNRRKLACIFLTAQVFSPCQITYNTIMIQLYFKTFLDLPEKSAFVYGSITELPFKFKHEFTCATRYVPWLPLHNLCSRTFTSMPPPPQSGDKSNKKSGPVTWKSLAVTFASGGVLLAGMKYFKKEKEEMIERERTKSLGKPALGGPFSLIDHNQKPKNSEDFFGQWILIYFGFTHCPDICPDELEKMIEVVDEIDRIPTLPNLTPLFITIDPERDTPEAILRYVKEFSPKLIGLTGTNEQVEKVSRAYRVYYSQGPKDEDNDYIVDHTIIMYLVGPNGDFIDYFGQNKKNTEIAATVASHMRKYKHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N312</accession>
    <name>W5N312_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015194474.1">
        <property type="nucleotide sequence ID" value="XM_015338988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N312"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015021"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015050.1">
        <property type="protein sequence ID" value="ENSLOCP00000015021.1"/>
        <property type="gene ID" value="ENSLOCG00000012209.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687474"/>
    <dbReference type="CTD" id="102687474"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155983"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_0_1"/>
    <dbReference type="InParanoid" id="W5N312"/>
    <dbReference type="OMA" id="FLSSWET"/>
    <dbReference type="OrthoDB" id="5859976at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012209">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:synapse"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="term" value="P:regulation of appetite"/>
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        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5-hydroxytryptamine receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001520">
        <property type="entry name" value="5HT4_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF146">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01059">
        <property type="entry name" value="5HT4RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="42"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="391" mass="44232" checksum="6199628E164C8B56" modified="2014-04-16" version="1">MATELNLTDISRNGEVNAELSPAERIILTIFLAFIIIMTVLGNLLVMVALCKDRQLRKKKTNYFIVSLAFADLLVAVVVMPFAAIELSKGYWEYGETFCLVRTSLDVMLTTASILHLCCIALDRYYAICCQPLVYRNKMTPLRVALMLGGCWVISFFISFLPIMQSWNAIGIEKIIEERKFNNSTNETYCVFLVNRPYAIICSAVAFYIPLVLMVLAYQRIYITAMEHVRQIGTLQRAGSAPEAYDGSDHQGSSRMKIETKAAKTLAVIMGCFCLCWAPFFITNIIDPFIDYSVPWELWTAWLWLGYVNSGLNPFLYAFLNRAFRRAFLMILCCGNERYVRPGSFGPSRHYTTSVNGTSIALRLAFLPNRNHSDNGRQILSSELESQDSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N425</accession>
    <name>W5N425_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SRY-box transcription factor 9a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000347">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_006635144.2"/>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015384"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015413.1">
        <property type="protein sequence ID" value="ENSLOCP00000015384.1"/>
        <property type="gene ID" value="ENSLOCG00000012498.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687702"/>
    <dbReference type="KEGG" id="loc:102687702"/>
    <dbReference type="CTD" id="60641"/>
    <dbReference type="eggNOG" id="KOG0527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158269"/>
    <dbReference type="HOGENOM" id="CLU_031800_0_0_1"/>
    <dbReference type="InParanoid" id="W5N425"/>
    <dbReference type="OMA" id="QSSNSYY"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012498">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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                <person name="Alfoldi J."/>
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                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
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                <person name="Lindblad-Toh K."/>
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</entry>
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                <person name="Alfoldi J."/>
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                <person name="Berlin A."/>
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                <person name="Jaffe D."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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            <begin position="60"/>
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            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="290" mass="33502" checksum="6B53F032C1F8AFBF" modified="2014-04-16" version="1">FNMNVFPTSVFLFFAFDSALVVWASVGDNFKDCKQFLYMRTPPVGIADGNLKKICQRYNNKPRFATLYDAARRVPVYSAYTFKKSDGQKRVDVPWMYEPQLGSPDEGGNMQALPQGEIDQLIEDSQAVLQDYADAVLYERGPLNPDEHQADNDDKAATYTLTNIVPQITTFFEESWSVYLDKIRKRLNNFCRGKSYIVTGITTSGSMIRRDHEDRLAVPKYLWSAYCCPRFDRNSPYDVRYMFPTYAAYGQNDQVDNSIIEVPLKTLESFLKNETDFDRNFNIFYKDCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7D1</accession>
    <name>W5N7D1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-retinol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.300</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01002277">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7D1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016540"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016570.1">
        <property type="protein sequence ID" value="ENSLOCP00000016540.1"/>
        <property type="gene ID" value="ENSLOCG00000013408.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164667"/>
    <dbReference type="HOGENOM" id="CLU_696309_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7D1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013408">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000145">
        <property type="entry name" value="Retinol dehydrogenase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004846">
        <property type="entry name" value="PRK06197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF69">
        <property type="entry name" value="RETINOL DEHYDROGENASE 12-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="396" mass="43772" checksum="CEA668571C719E77" modified="2014-04-16" version="1">MGRIQFDDVNSERSYHPVKAYVQSKLANVLFTRELASRLQGTGVTAYAVDPGIVYTDAIRHMKTFFQVFIKAFSFLIKTPAQGAYTTLYCVLTPDLESGGYYRNFFCGPWSSTVRLEGKTVLITGANTGIGKETARDLARRGARVIMACRDLEKAEGAQKEIIQDSGNQEVVVKKLDLSDTKSIREFAEQVNKEEKQVNILINNAGIMMCPYSKTADGFEMQFGVNHLGHFLLTHLLIDLVKRSAPARIINVSSMAHSWGRIQLEDINSEKSYDSGRAYGQSKLANILFTRRLAEKLQGTGVTVYSLHPGVVQTELGRHLGGPMQLAMKVVKPFSKTSVQGALTSIYCAVAPELEKESGGYYSDCAPASCSRAAKDDEMAKKLWDSSCQMLGISWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9D1</accession>
    <name>W5N9D1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9D1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017240"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017271.1">
        <property type="protein sequence ID" value="ENSLOCP00000017240.1"/>
        <property type="gene ID" value="ENSLOCG00000013989.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RYAR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165100"/>
    <dbReference type="HOGENOM" id="CLU_076370_2_3_1"/>
    <dbReference type="InParanoid" id="W5N9D1"/>
    <dbReference type="OMA" id="CCCCISF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013989">
        <property type="expression patterns" value="Expressed in intestine"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="126" mass="13515" checksum="37061C6047ECC804" modified="2014-04-16" version="1">MNDIAEMVAFVLGFLGWVMAGVAVPNRYWKESTLEGSVITTTTIYENLWISCASDASGIHNCREFPSLLALSGYLQVSRALMVTSIVLGFCATLVSMLGLQCTKVGGDNYTLKGRLAGLGGLLFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAM1</accession>
    <name>W5NAM1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">P1, F-box associated domain containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029369">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAM1"/>
    <dbReference type="FunCoup" id="W5NAM1">
        <property type="interactions" value="750"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017680"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017712.1">
        <property type="protein sequence ID" value="ENSLOCP00000017680.1"/>
        <property type="gene ID" value="ENSLOCG00000014359.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161313"/>
    <dbReference type="HOGENOM" id="CLU_068548_1_0_1"/>
    <dbReference type="InParanoid" id="W5NAM1"/>
    <dbReference type="OMA" id="QQFEGAW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014359">
        <property type="expression patterns" value="Expressed in mesonephros and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000012">
        <property type="entry name" value="F-box only protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007397">
        <property type="entry name" value="F-box-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039752">
        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04300">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="194" mass="21683" checksum="112B9F7E05479B0C" modified="2014-04-16" version="1">PPGLSHTTPPPEHELPGMFGAEPPRSAPEGDFTGWTTSTEVLPYDTSGIPEGAAVCALPQYSNVWCTWSKVVSMHSHFNWPPLGSAPLTRAAAAALALRRYEESRVHEFIYQLRVRLLGADGRTVLQEHAFAPKEDMRRDSRDWKKVSHVFRSYGPGVRHVHFLHRLKNMNMVAFRATRATDSAVLVKLVKNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAY0</accession>
    <name>W5NAY0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01038663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAY0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017789"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017821.1">
        <property type="protein sequence ID" value="ENSLOCP00000017789.1"/>
        <property type="gene ID" value="ENSLOCG00000014455.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU54">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163253"/>
    <dbReference type="HOGENOM" id="CLU_135105_0_0_1"/>
    <dbReference type="InParanoid" id="W5NAY0"/>
    <dbReference type="OMA" id="MCGRRRE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014455">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
        <property type="entry name" value="ZP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ZP" evidence="13">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="17682" checksum="0C4B313FD70B883E" modified="2014-04-16" version="1">YDSYYSDLDYPVTKVLRDPVYVEVHILNRTDPNIVLTLGDCWATSTPSPLSQSRWSLLVAGCPYGGDNYQTTLIPVGGSSGLPYPTHYQRFMLRMFTFVDPASQVPLMEKVFIHCSAAVCQPTATDRCVPMCGRRRELPISTKACFFVRSSVFCFMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBM8</accession>
    <name>W5NBM8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MALT paracaspase 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004938">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004939">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBM8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018037"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018069.1">
        <property type="protein sequence ID" value="ENSLOCP00000018037.1"/>
        <property type="gene ID" value="ENSLOCG00000014651.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUZM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018044"/>
    <dbReference type="HOGENOM" id="CLU_014796_0_0_1"/>
    <dbReference type="InParanoid" id="W5NBM8"/>
    <dbReference type="OMA" id="GYACEDA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014651">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052039">
        <property type="entry name" value="Caspase-related_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041077">
        <property type="entry name" value="MALT1_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033540">
        <property type="entry name" value="MALT1_IG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576">
        <property type="entry name" value="MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1/PARACASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576:SF38">
        <property type="entry name" value="MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18703">
        <property type="entry name" value="MALT1_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="2">
        <location>
            <begin position="196"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="628" mass="70254" checksum="92B91588355990F5" modified="2014-04-16" version="1">MIKDIVITEHPVSVCVPQNYPVTLSCRAIGKGTLQYQWFQAENEEVPGAAHPDLVTTAKKTQVYICRVNDQHSNCVFSDWVKVKVRDVLRPGIPAPWLGEPHIVAHPTSLSVRPGETAALQCTAFGIPAPHYQWYKNGNLMQGETGETLLIRNANKNQEGTYLCSVSNVHEEKWTEPADSETLPPDKNCWSVGLATDKVALLIGNLNYSNHPNLIAPMIDVHELANLLQQLGFRVVSLLDLTKQEMCAVIDEFLQLLDRGVYALFYYAGHGYECPGRNYLVPIDAPQPYRRENCISVQRIMRSMQEKRTALNVVLLDTCRKWYNQGCVPSEIRSLAPLGNTVYGYATCEDAEAYEVQDGERSSGIFTKYLNKHILSPEKVTRVLERVLEDLGRDPLITGKQVMEIKHTLKDPRALTDRICTTGHTMELQLRNLYWKHTNELPERRVLVFPCGAEVELSFSALFSNVMIAFASLKRAGRGAADCSLSLRSEPAMEDVFSNMEDRSGGMDSLLLSGADNPDSSLRLCGLQNLQKSLVIRLDLHYTDSDSKLRRVETREEDIGKPLVARSELHRRGQGRGPAARECRAAGASFRPSTRKAENARDPVSKRHTAQGNEPEENDENEPLDCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCH8</accession>
    <name>W5NCH8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 711</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcription regulator required for brain development. Probably acts as a transcription factor that binds to the promoter of target genes and recruits PHF8 histone demethylase, leading to activated expression of genes involved in neuron development, such as KDM5C. May compete with transcription factor ARX for activation of expression of KDM5C.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with PHF8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016799">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCH8"/>
    <dbReference type="FunCoup" id="W5NCH8">
        <property type="interactions" value="352"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018337"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018369.1">
        <property type="protein sequence ID" value="ENSLOCP00000018337.1"/>
        <property type="gene ID" value="ENSLOCG00000014891.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159611"/>
    <dbReference type="InParanoid" id="W5NCH8"/>
    <dbReference type="OMA" id="QEDDISC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014891">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000054">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000179">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000209">
        <property type="entry name" value="Zinc finger protein 711"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000170">
        <property type="entry name" value="Zinc finger protein 711 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006794">
        <property type="entry name" value="Transcrp_activ_Zfx/Zfy-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF140">
        <property type="entry name" value="ZINC FINGER Y-CHROMOSOMAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04704">
        <property type="entry name" value="Zfx_Zfy_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="397"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="547"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="604"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="661"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="690"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="718"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="747"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="775" mass="87461" checksum="5941C0EBF5D83A0F" modified="2014-04-16" version="1">MDQGGGGLELQSQDSKMPHTMIMQDFVAGMGGMAHIDGDHIVVSVPEAVLVSDVVTDEGITLEHGLEAEVVEGPEIGQGDVVTADGVIVPESVLGAEVAIEEALDADHHVLTTDLIAESVRVPDQVFVADLVSHQDGHLGHEMVSEEVMVTNSDSETVIQAHEGVPASTVTIKTEDDEDGKSTSEDYLMISLDDVGEKLDIGNTPLKISTEVTQDDGSKEDGFGSEVIKVYIFKAEAEDDVEIGGTEVVTESDFHNGHAVLEPGSVGRLPREKMVYMAVKDTSQEDEDINCAEIADEVYMEVIVGEEEAPAMQETQLDDSTVNKTFVPVAWAAAYGNNLDTRLENKNGAATQYLQISDSISTSRALKQKAKKKKRGETRQCQTAVIIGPDGQPLTVYPCHICGKKFKSRGFLKRHMKNHPDHMFKKKYQCTDCDFTTNKKASFHNHLESHKLIIKTEKIHEFTEYTRRYREASPLSSNKLILRDKEPKLHKCKYCDYETAEQGLLNRHLLAVHSKNFAHVCVECAKGFRHPSELKKHMRTHTGEKPYQCQHCEFRCADQSNLKTHIKSKHGTDLPYKCGHCPQAFADDKELQKHTEIFQGHKTHQCPHCDHKSTNSSDLKRHIISVHTKDFPHKCEVCEKGFHRPSELKKHSETHKGKKVHQCRHCDFKISDPFTLSRHILSVHTKDLPFKCKRCKRGFRQQNELKKHMKTHSGRKVYQCQYCEYNTTDASGFKRHVISIHTKDYPHRCEYCKKGFRRPSEKNQHIMRHHKDVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDC6</accession>
    <name>W5NDC6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004842">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDC6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018635"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018667.1">
        <property type="protein sequence ID" value="ENSLOCP00000018635.1"/>
        <property type="gene ID" value="ENSLOCG00000015141.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154201"/>
    <dbReference type="HOGENOM" id="CLU_015367_0_1_1"/>
    <dbReference type="InParanoid" id="W5NDC6"/>
    <dbReference type="OMA" id="GAYSHQM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015141">
        <property type="expression patterns" value="Expressed in brain and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12635">
        <property type="entry name" value="RRM2_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12639">
        <property type="entry name" value="RRM3_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000007">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000069">
        <property type="entry name" value="CUGBP Elav-like family member 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="295"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="40786" checksum="3321CDFCD1BBDE2F" modified="2014-04-16" version="1">MNRPIQVKPADSEGRGEDRKLFVGMLGKQQSEEDVRRLFEPFGQIEECTILRGPDGASKGCAFVKFSSHAEAQAAINNLHGGQTMPGASSSLVVKFADTDKERTLRRMHQMAGQLGIFSPMTIQFGAYGAYTHAVSDISPWQQIMQQQAALMAAAQGSYLNPMAAIAAAQMQQMAAFNVNGLVAAPMTPSSGTSTPPGISATAVPSIATPIGVNGFSTLPPQSNGQPASEPIYTNGIHPYPAQSPTVADPLQQAYAGVQHYAAAAYPTAYAPISQAFPQQPAIIPQQQREGPEGCNLFIYHLPQEFGDAELMQMFLPFGNVISAKVFVDRATNQSKCFGFVSFDNPSSAQAAIQAMNGFQIGMKRLKVQLKRPKDANRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDM6</accession>
    <name>W5NDM6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pre-mRNA 3'-end-processing factor FIP1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017029">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDM6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018735"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018767.1">
        <property type="protein sequence ID" value="ENSLOCP00000018735.1"/>
        <property type="gene ID" value="ENSLOCG00000015223.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162578"/>
    <dbReference type="HOGENOM" id="CLU_035577_1_0_1"/>
    <dbReference type="InParanoid" id="W5NDM6"/>
    <dbReference type="OMA" id="QHSRAGF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015223">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF8">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="6">
        <location>
            <begin position="145"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="581" mass="63510" checksum="DC0EEA4A06B83C66" modified="2014-04-16" version="1">MATEVGTSAPSSIAHPGVEPEEDEEHWLYGDENSEKQPGDPAPGFPDSTQESHVSSSEDKDTADQVAQSGEDEDEEDSDSDSDEDDVKVTIGNIKTGAPSYMATPMNLSLKTGRGYGAAATAKLQPKGVDLEATGNINGLPALEADPDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQRRLQLGLDPAGPAATENKITVQQGRMPVLEKDVDLAAIKPEFKADFPPTPVPLPPGRVKAGPPPNRKLGGTIDVIGGQTGAIRRVEGRRREKHASDENPIQVPAPGERGPPEAGPPPPPIGAPPPHFLHPPPPVTSVPPPLHPPGSASAVASRVPLRHGGLTAGIPPPAIPGLFPPPMAPPPALLMPPLDGCPTSAYGSRPPPPFGYSSGDSSFVSYPPISTSLTPWVTSVEKGSSGAGSAGHWEYQGSRRDRERERDRTPTTSEYSKYGRRLRRGWGGRESRHDGSYAAPSPRSEDERYRYYSRERSCEYEREYHHGRERSREREERHRERRHRDKEDGSKHKSSRRVSCHPTRKQHDGEEGESHRRHKHKKNKRSKEDKEAAEDGAGEGEEPDSKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDS2</accession>
    <name>W5NDS2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synembryn</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein Ric-8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with some GDP-bound G alpha proteins. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDS2"/>
    <dbReference type="FunCoup" id="W5NDS2">
        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018781"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018813.1">
        <property type="protein sequence ID" value="ENSLOCP00000018781.1"/>
        <property type="gene ID" value="ENSLOCG00000015262.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4464">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014700"/>
    <dbReference type="InParanoid" id="W5NDS2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015262">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000447">
        <property type="entry name" value="RIC8 guanine nucleotide exchange factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF2">
        <property type="entry name" value="SYNEMBRYN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="528" mass="58843" checksum="32F5BF91198862D8" modified="2014-04-16" version="1">MDLSIILSNLETGNEEEIETLLREFNRENCRTFTFDQKEEDKRKKLCAGLVRVLERDGSPCCQTACLEALRILSRDKRVLGPVATQEGMLVLARRGCLREAPDTPHERVAVEALKCLCNVVFNSQEAQQVSADIRLAAGLCAQLRTSAACGLSHEVSLFSLRLLFLLSALRTDVRALLQHELKAVDLLVEILEHTLSIKWAGKYEAAMDPDPKPLPLEENERAMEALKALFNLTLCDTREEDGAHQLRLITAIMRHLLMIKTQTEDKTEEAHSHAINLLSNIPVSCLDVLINVQSQGGLEEYNGKNMDVIQVLLDFMEKRIDKGSNYKEGLTPVLSLLTESSRHHREIRKYIKSQVLPPLKDVKNRPEVGTTVRNKLVRLMTHVDTGVKQSAAEFLFVLCKESVDNLLKYTGYGNAAGLLAARGLLAGGRGEGQYSDEEDSDTEEYKSAKPFFHPVQSGHTFQHTAGCVCQQEETKMEYVSLSLSSFSPFTCRQQIIRPMGVRRDGTLAPLAEAVQQCSADSCSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDW9</accession>
    <name>W5NDW9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01006120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01006122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDW9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018828"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018860.1">
        <property type="protein sequence ID" value="ENSLOCP00000018828.1"/>
        <property type="gene ID" value="ENSLOCG00000015299.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0033">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158973"/>
    <dbReference type="HOGENOM" id="CLU_000288_71_3_1"/>
    <dbReference type="InParanoid" id="W5NDW9"/>
    <dbReference type="OMA" id="WERTKIS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015299">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048168">
        <property type="term" value="P:regulation of neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903076">
        <property type="term" value="P:regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="349"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="506"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="480"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="523"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="692" mass="75372" checksum="61092C03D9DD1E5A" modified="2014-04-16" version="1">MATTTCTRFTDEYQLYEELGKGAFSVVRRCVKLCTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLLFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKNAAVKLADFGLAIEVQGDQQAWFAGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAQEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATVTAAAAGTAAGLVEQAAKSLLNKKADVKPQSNSTKNSIVTSPKGNLPSPALEPQTTVIHNPVDGIKESSDSSNTTIEDEDVKVSPLPDRTGKSLDSSSVKAQSSSGSHSEAVQSPQSPPPAVFSSKLTDILGCVRRGSGPGSDSEGGASSAPAPQQGPLPPHTPVIPAPASRLTDIVGSVRRGSAPHSDSEGVSARPSVPPPRSSRPSVPPPRSSPPPLPPSSPSMSPQSSSSARKQEIIKITEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLSKNSKPIHTTILNPHVHLIGEDAACIAYIRLTQFVDAQGRPRSSQSEETRVWHRRDSKWQNVHFHCSGAPAAPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEX8</accession>
    <name>W5NEX8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 32">Cystic fibrosis transmembrane conductance regulator</fullName>
            <ecNumber evidence="6 32">5.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26 32">ATP-binding cassette sub-family C member 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27 32">Channel conductance-controlling ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28 32">cAMP-dependent chloride channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="32">Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane. Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains. The ion channel is also permeable to HCO(3)(-); selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Contributes to the regulation of the pH and the ion content of the epithelial fluid layer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29 32">
            <text>ATP + H2O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel.</text>
            <dbReference type="EC" id="5.6.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>hydrogencarbonate(in) = hydrogencarbonate(out)</text>
            <dbReference type="Rhea" id="RHEA:28695"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Cell membrane</location>
            <topology evidence="32">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 32">Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="102"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSLOCP00000019187.1"/>
        <property type="gene ID" value="ENSLOCG00000015585.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015585">
        <property type="expression patterns" value="Expressed in intestine and 8 other cell types or tissues"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="term" value="C:basolateral plasma membrane"/>
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        <property type="term" value="C:chloride channel complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005260">
        <property type="term" value="F:intracellularly ATP-gated chloride channel activity"/>
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    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
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    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008354">
        <property type="term" value="P:germ cell migration"/>
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        <property type="term" value="P:heart development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:Kupffer's vesicle development"/>
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        <property type="term" value="P:lymphoid lineage cell migration into thymus"/>
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    <dbReference type="GO" id="GO:0031016">
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        <property type="term" value="P:regulation of neutrophil chemotaxis"/>
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    <dbReference type="GO" id="GO:0060063">
        <property type="term" value="P:Spemann organizer formation at the embryonic shield"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:T cell differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd18594">
        <property type="entry name" value="ABC_6TM_CFTR_D1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystic fibrosis transmembrane conductance regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
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        <property type="entry name" value="ABC1_TM_sf"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
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        <property type="entry name" value="CFTR/ABCC7"/>
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        <property type="entry name" value="CFTR_reg_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR"/>
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        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CYSFIBREGLTR"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 32" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22 32" id="KW-0868">Chloride</keyword>
    <keyword evidence="20 32" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="24 32" id="KW-0407">Ion channel</keyword>
    <keyword evidence="18 32" id="KW-0406">Ion transport</keyword>
    <keyword evidence="23" id="KW-0413">Isomerase</keyword>
    <keyword evidence="19 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 32" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 32" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 32" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="104"/>
            <end position="123"/>
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    <feature type="transmembrane region" description="Helical" evidence="32">
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    <feature type="transmembrane region" description="Helical" evidence="32">
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            <begin position="226"/>
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    <feature type="transmembrane region" description="Helical" evidence="32">
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            <begin position="253"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="336"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="884"/>
            <end position="909"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="930"/>
            <end position="948"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="1127"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="1159"/>
            <end position="1183"/>
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    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="35">
        <location>
            <begin position="129"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="34">
        <location>
            <begin position="455"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="35">
        <location>
            <begin position="887"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="34">
        <location>
            <begin position="1234"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1477"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="33">
        <location>
            <begin position="1477"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="33">
        <location>
            <begin position="1497"/>
            <end position="1508"/>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00012195"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00016668"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00029720"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00031358"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034073"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00044653"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
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    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="RuleBase" id="RU362037"/>
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    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
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    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
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    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019187.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1508" mass="172122" checksum="00E3B7532DB02697" modified="2014-04-16" version="1">MQKSPAEDANAFSRFFFWLSRVIGRGYEFTIDTIDMYDICHKSVNVDLWWASPILRKGYRQKLELSDVYQVPSFDSADHLSERLEREWDRELASSKKNPNLLNAIRRCFFWPFAFYGVLLYFGEASKSVQPQLLGRIIASFDPFYETERSQGYYMALGLSLLFITRSLLLQSAIFGLHHLGMQIRIALFSLIYKKTLKLSSRVLDKISTGQLVSLMSANLNKFDESLGLAHFVWITPLQCMLLMWFIWDLIEASSFCALASLTLLAIAQACLSHRMDPYRAKRAKKISERLKLTSEIIENIHSVKAYGWEEIMEKIIQNIRQDEMHLTRKIGYLRYFYSATYFFSAFLVVICAVVPYALSKGITLRKIFTTMSYCMVLRMTLTRQFPGSVQMWYDTLALINKIEDFLKKEEYKVLDYNLTTTEVELVNVTASWDEGIGELFEKIKQENKDSGNPNGEAGLFFTNLYVTPVLKNISLYLEKGKMLAVAGSTGSGKSSLLMMIMGELEPSEGKIKHSGRISYSPQTSWIIPGTIRENIVFGLTYDEYRYTSVIKACQLEEDLALLPDKDKTRLGEGGVTLSGGQRDRIGLARAVYKDADLYLLDSPFTHLDIVTEKEIFERCLCRLMATKTRVVVTSKLDHLKRADKILLLHNGTTYFYGTFSELKSQRPDFTSLLIGLEAYDNINAERRGSILDETLRRVSVDDTAGFHPEPTRQSFKQATIDQTEKRRKSSVIINPLAASRKFSIIQPEEKSVSELGERRFSFIPENEQVEEALPRSNVLDRGQTFHGQRRQSVLAFITNAQSQGRREHLTSSFARKLSITPQCDLASELDIYARRLSKDSIYEINEDVDEEDMEECFADERENVFETTSWSTCFRYMMTNKSLVYVLIFIVFVFIFEAGGSLVGYYFIKDILINDTNSQPGNSSSPGTHYAVIATPTSSYYIFYIWVATSETVFAMGFCRGIPLIHTLLTISKRLHEQMLSSVLRAPMAILNTMKTGRIMNRFTKDLAVIDDMLPLILFDFVQLVLIVVGAIFVVSIIQPYIFISAVPLILIFVLLRKYFLRTGQQLKQLEAEARTPIFSHLIISLKGLWTIRAFGRQSYFEALFHKALNVHTANWFLYLSTLRWFLFRIDMVFVFFFSAVAFIAVATNHSGDKPGEIGIIIAMAMLLMGTFQWTVITSITVDSLMRSVNRVFKFIDLPSEEHKSSKRGKKAADLVIENVHANDDDWPSRGHMKVSNLTVRYTEGGRAVLQNLSFSVNRGERVGVLGRTGSGKSTLLSALLRLASTEGDITIDGVSWNTVPLQRWRKAFGVVPQKIFILSGTFRMNLDPYGRWSDEELWRVTEEVGLKSVIEQFPDKLDFQLVDGGYVLSNGYKQLMCLARSILSKAKILLLDEPSAHLDPINRTLQVLKKTLKHSFSNCTVMLSEHRVEPLMECQSFLMIEGSSVKPYDSIQKLLNETTQLKQAISNAERMKLFPLHRRNSSKRKSKPKITALQEEAEDEVQDTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF44</accession>
    <name>W5NF44_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Plexin A4</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016147">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01016148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NF44">
        <property type="interactions" value="377"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019253"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019285.1">
        <property type="protein sequence ID" value="ENSLOCP00000019253.1"/>
        <property type="gene ID" value="ENSLOCG00000015635.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3610">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244850"/>
    <dbReference type="HOGENOM" id="CLU_001436_1_1_1"/>
    <dbReference type="InParanoid" id="W5NF44"/>
    <dbReference type="OMA" id="HESRWLE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015635">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007414">
        <property type="term" value="P:axonal defasciculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001763">
        <property type="term" value="P:morphogenesis of a branching structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01181">
        <property type="entry name" value="IPT_plexin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000329">
        <property type="entry name" value="Plexin A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF34">
        <property type="entry name" value="PLEXIN-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869248" evidence="13">
        <location>
            <begin position="27"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="21"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1263"/>
            <end position="1290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1893" mass="212987" checksum="A9BDE9ECEEFF15FD" modified="2014-04-16" version="1">MASFMRNWTFLTFYILPILTASSASRQPVHINKEFSTFHSENSEWTFNHLAVDARNGNVYLGAVNRIYKLSSDLKVQVTHQTGPDEDNPKCYPPRIVQPCHEPLTLTNNINKMLLIDYKENRLLACGSLYQGICKLLRLEDLFKLGEPSHKKEHYLSGVNDSGSVFGVIVSNKDSADKLFIATAVDGKPEYFPTISSRKLTRNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDFDIYYVYGFSSGNFVYFLTLQPEMTSPTGSATKEQVYTSKLVRLCKEDTAFNSYVEVPIGCMKNGIEYRLLQAAYLSKAGAILARSLGISPDDDVLYTIFSKGQKRRTKSPEESALCVFMLKNINDRIKDRLQSCYKGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSELVRGIPLFSEESDRMTSVIAYVYKNHSLAFIGTRSGKLKKIRVDGPSHGALQYETVQVVENGPILRDMAFSADQHYLYIMSENQLTRVPVESCGQYTTCSECLGSGDPHCGWCVLHNTCTRKEYCERSTEPRRFASHIKQCVRLSVHPNNISVSQYSVLLILEAHNVPELSAGVNCTFEDLAEMDGLVEGNRIKCNSPAEKEVPQIIIDKGDHQIVQLYLKSKETGLAFANTSFVFYNCSVHKSCLSCVNSPYQCHWCKYRHMCTHDPRSCSFQEGRVKHSEDCPQLLPAEKILVPVGVVKPITLKAKNLPQPQSGQRGYECILNIQGTEERVPALRFNSSSVQCQNTSYSYEGMEINSLPVDLMVVWNGDFSIDNPADNKVHLYKCGAMRESCGLCLKADPHFGCVWCKRENRCTLKQHCPQLQNQWLENTGLNSKCTHPKITQITPLRGPREGGTLVTIRGENLGLEFREIEGHVKVAEVDCTPVPEGYIPAEQIVCEMGKAERSQYAGSVQVCVGECRPEFMATSYKYYFFVVPKLMYLKPNRGPVSGGTIVNITGSNLDAGSNVSIMFKDQKCIFHSNRRGAQWITCKSQASLHGYGNVTVSVRVDRAELQKDLRFEYVEDPIITKLEPEWSIFSGNTPITVTGTNLDIIQNPLIRAKYNGVETVNTCKMLSSTSMVCQAPELAVSSSHQGEVVERPDEFGFILDNVQSVLSLNSTNFIYYPNPVFEVLSLSGVLELKPGSPIILKGRNFLPPAPGGNAKLNYTVLIGEKPCTLTVSDTQLLCESPNLTGRHKVLARVGGIEFSPGVVHISSDSPLSHTAIISISSAGGLLIIFIVVVLIAYKRKSRESDLTLKRLQMQMDNLESRVALECKEAFAELQTDIHELTSDLDGAGIPFLDYRTYTMRVLFPGIEEHPVLRDLEVPGYRQEQVEKGLKLFGQLINNKVFLLSFIRTLESQRSFSMRDRGNVASLIMTVLQSKLEYATDVLKQLLSDLIDKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFSLFCAIKQQMEKGPIDSITGEARYSLSEDKLIRQQIDYKTLILNCLNPDNEKSPEIPVKVLNCDTITQVKEKILDAIYKNVPYSHRPKASDMDLEWRQGRGSRLILQDEDVTTKIENDWKRLNMLAHYQVPDNSVVALVPKQVMAYNSVNNSTVSRTSASKYENMIRYTGSPDSLRSRTPMITPDLESGVKVWHLVKNHEHGDQKEGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHSIHDPHVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKSSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKSWVERYYSDIGKMPAISDQDMNAYLAEQSRMHMNEFNTMSSLSEIYSYVGKYSEEIVSALEQDDAARKQRLAFKLEQVVTLMSIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFW7</accession>
    <name>W5NFW7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hyaluronidase</fullName>
            <ecNumber evidence="10">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 10">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01035612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01035613">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFW7"/>
    <dbReference type="FunCoup" id="W5NFW7">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019526"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019558.1">
        <property type="protein sequence ID" value="ENSLOCP00000019526.1"/>
        <property type="gene ID" value="ENSLOCG00000015859.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPZH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230364"/>
    <dbReference type="HOGENOM" id="CLU_036366_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFW7"/>
    <dbReference type="OMA" id="CAKAFMK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015859">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF7">
        <property type="entry name" value="HYALURONIDASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00848">
        <property type="entry name" value="SPERMPH20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="8">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="61"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="383"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="448" mass="51116" checksum="A3015F7CF6DD18B8" modified="2014-04-16" version="1">CYVVSLHTLQINRLSCVLILILTCLWALILISSSFALKPAKLPLIGRKPFIAAWNAPFDLCTTKYNISVNLEMFHISGSPRASRRGQNVTIFYANRLGHYPFYTEEGLPVNGGLPQNCSLETHLQKADKDIKFYIPSENFSGLAIIDWEYWRPQWLRNWQKKDIYRRKSRDLISKGYINVTSQQIEYLAQARFERSAMAFMKRTIQLGIQTRPHGLWGYYLYPDCHNYNLLDNNYTGSCPVLESLRNDELFWLWNSSTALFPSIAIKKSHADSVSNLYFAQFRVLESLRIASMTSMDYDLPTFVYTRLGYRDEALSFLSTKDLIHTIGESAALGAAGFVIWGDVNLTSSRASCEALSRYLPSTLNPYIVNVTAAAKLCSDMLCQGNGRCVRKNYDSNDYLHLNPANFKIQRGSNGKYFAVGMTSSADLTIFMNKFTCRCYAVRTFHHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGB8</accession>
    <name>W5NGB8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine-hydroxylase NDUFAF5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putative methyltransferase NDUFAF5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGB8"/>
    <dbReference type="FunCoup" id="W5NGB8">
        <property type="interactions" value="941"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019677"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019709.1">
        <property type="protein sequence ID" value="ENSLOCP00000019677.1"/>
        <property type="gene ID" value="ENSLOCG00000015981.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014687"/>
    <dbReference type="HOGENOM" id="CLU_046586_0_2_1"/>
    <dbReference type="InParanoid" id="W5NGB8"/>
    <dbReference type="OMA" id="YEVVYGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015981">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000199">
        <property type="entry name" value="arginine-hydroxylase NDUFAF5, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="12">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="360" mass="40549" checksum="A49ADFF75F1271E6" modified="2014-04-16" version="1">LDNMNNVFIVRRAFSTSCMLCPWRRARKVVLSSGQLAHQSQGLVFKGCTNFKRWFSSRTANSMNIFDRNMKRRQKNWAAALQDGDKYHYLRDEVGSRVADRVFDIARTFPLALDIGCGASHIALHLNKETIEKLFLMDVTVNALKLKETEIPTYRVLADEEFLPFKENTFDLVVSSLSLHWINDLPGAFRQIHHVLKPDGVFIGAMVGGETLYELRCSLQLAELEREGGFAPHVSPYTAVTDLGNLLGQAGFNMLTVDIDDIQVYYPGMFEVLNDLQGMGESNCAWNRKPMLHRDTMLAAAAIYKEMYGSEDGSVPATFEILYMIGWKPHESQAKPAKRGSANMSFGDLGKIIQPPSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGG6</accession>
    <name>W5NGG6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Atrophin-1-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">BAI1-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Membrane-associated guanylate kinase inverted 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Membrane-associated guanylate kinase inverted 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Plays a role in coupling actin fibers to cell junctions in endothelial cells, via its interaction with AMOTL2 and CDH5. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Cilium</location>
            <location evidence="4">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Synapse</location>
            <location evidence="19">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021047">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021048">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021051">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021052">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021053">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NGG6">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019725"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019757.1">
        <property type="protein sequence ID" value="ENSLOCP00000019725.1"/>
        <property type="gene ID" value="ENSLOCG00000016013.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155057"/>
    <dbReference type="HOGENOM" id="CLU_004562_1_0_1"/>
    <dbReference type="InParanoid" id="W5NGG6"/>
    <dbReference type="OMA" id="YLRTVPX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016013">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031697">
        <property type="term" value="F:beta-1 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070699">
        <property type="term" value="F:type II activin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06731">
        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000006">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000015">
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    <dbReference type="FunFam" id="2.30.42.10:FF:000113">
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    <dbReference type="FunFam" id="2.30.42.10:FF:000150">
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    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
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    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF27">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16663">
        <property type="entry name" value="MAGI_u1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
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    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="WW" evidence="28">
        <location>
            <begin position="140"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="28">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="29">
        <location>
            <begin position="265"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="29">
        <location>
            <begin position="444"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="29">
        <location>
            <begin position="625"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="29">
        <location>
            <begin position="768"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="29">
        <location>
            <begin position="998"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="710"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="931"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1083"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="722"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1133"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1230"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1257"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1271"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00070827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00079516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1307" mass="144304" checksum="1EBDD66226E0B1CA" modified="2014-04-16" version="1">MELEKSGALLESGTYEDNYYGTPKPPAEPTPLLLNVTDQILPGARPSAEGKRKRNKSVSNMEKAGIDPPEEEEEERPVVNGNGVAITPESSEHEDKSTDASGEIPPQPYTTPTFGHTEGPKEEGEPAKQPTKPDENEELGPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKEDELPYGWEKIDDPIYGSYYVDHINRRTQFENPVLEAKRRLQQQQMPNQDLSSQPLQAVQVFREKPLFTRDATQLKGTFLSTALKKSSMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMDTGDVIVYINDVCVLGHTHADVVKLFQSVPIGQSVSLVLCRGYPLPYDPEDPASSMISPMGMMERPPVLVNGRSNYDNYMEYLSRTSRFMQDHTEQLPQQSLPPHPGDTHLDGQLPPPIHEDNVSMASSGATQSELMTITIVKGADGFGFTIADSPTGQRVKQILDAQGCPGLCEGDLIVEINQQNVQSLSHTQVVDLLKECPMGSETSLVIQRGGECHFSPWKTSKQVLEQWDVQGSPQTSLSAPVIPQNVPFPSNFHRASFPDSADGFDQRKPDPYELYEKSRAIYESRQQGPPRTAFRMDSTGSHLMLGLEYRELDVHLRRQKSGFGFRILGGDEPGQPILIGAIIEKSSADRDGRLRPGDELVFVDGIPVAGKTHRYVIDLMHGAARNGQVNLTVKRKVQGTGEPCPENGRSPGSVSTQHSSPRSDFTATTFPNNTHQPNAGTSSPEGFVSHSLQTNDVVIHRKENEGFGFVIISSLNRPESGSTITVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLRIIPQEELSNNYTRPRDQLQVAVMWIPQYPTEHITKISSCRTASIKTCVVLLRLPVAQRYYYLDSYRSEVKARQDVKPDIRQPPFTDYRQPPVDYRHPPVADYRQPPTLDYRHPPLLDYRQHSPDARQFPIPDYRQPQVRDFDYFTVELEKSMKGFGFSIRGGREYKMDLFVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVRLLLKRGTGQVPEYADSPTPWDAPSTTSPTLPEVAPCLDTMTMPLPSSHPALPSDPIHLLPLERGKETSRSMEKAEHSQSPRKADQLKPVQASGKRSAERSGGGKSKEGGRPTSGGSAIRGKGTPEDVTDQAKLRGAAPEQQDQPKASKTTRGRSKERKAESATASEKRPAERSSRGIKLDLNGGAQSNGKNPGSTTEPAKEKRDGEPTRISGSGAARKSTVSPGPWKIPGSDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHL4</accession>
    <name>W5NHL4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 16">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001676">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHL4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020123"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020156.1">
        <property type="protein sequence ID" value="ENSLOCP00000020123.1"/>
        <property type="gene ID" value="ENSLOCG00000016301.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074867"/>
    <dbReference type="InParanoid" id="W5NHL4"/>
    <dbReference type="OMA" id="LVRFPQK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016301">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002204">
        <property type="term" value="P:somatic recombination of immunoglobulin genes involved in immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03285">
        <property type="entry name" value="ABC_MSH2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000009">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000523">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032642">
        <property type="entry name" value="Msh2_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 15 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 15 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="18">
        <location>
            <begin position="742"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="937" mass="104544" checksum="02684F64731D1097" modified="2014-04-16" version="1">MAVQPKQNLSMDSAAENGFVHFYYSMPEKPDTTVRVFDRNEYYTVHGKDALFAAKEVFKTNGVIKNLGSGSRKLESVVLSKMNFESFMRDLLLVRQYRVEVYRNKASSKSGKDHDWQLACKASPGNLTQFEEILFGSSSPEGAAGVVGIRLGSGDGQRTVGVGYVDSTLRRLGVLEFPDNDQFSNLEALLVQIGPKECVLPSGETQGDMTRLRQVVQQGGMLVSDRKRAEFSTKDIVQDLNRLLRTKKGEQASSAALPEMEKQIAMSSVAAVIKYLELLSDESNFNSFEMTTLDLSQYMRLDNAAVRALNLFQASPDDTSGSHSLAGLLNKCKTPQGQRLVNQWIKQPLMDKNKIEERLDLVESFFHDVELRQSCQEDLLRRFPDLNRLAKKFQRQTATLQDCYRVYQAVSQLPNLVLALQRYSGKHQILLNAVFITSLNDLTSDFSKYQEMIETTLDMNQVEHHEFLVKPSFDPNLSELREKMDQLESSMQSALNSAARELGLDAGKTVKLESNAQLGYYFRVTCKEEKSLRNNRKFTTLDVQKNGVRFTNSKLSSLNEEYTRSREEYEEAQNAIVKEIISISTGYVEPIQILNDVIAQLDAVVSFAQVSNAAPVPYVRPVMLEKGSGKLSLQGARHPCIEAQDDIVFIPNDVSFIRGQETFHIITGPNMGGKSTYIRQVGVIVLMAQIGCFVPCDSAEISIVDCVLARVGAGDSTLKGVSTFMAEMLETASILRSATEDSLIIVDELGRGTSTYDGFGLAWAISEYIATRIKAFCFFATHFHELTALAEQVSTVSNLHVTALTTDNTLTMLYRVKKGVCDQSFGIHVAELASFPKHVIESAKEKALELEEFQHVERSDEGGEGPAAKRSRLDRQEGEKLIEDFLSKVKALPVSDMSEEEIKEELKKMKADILTKNNGFVNVIVARSKNVPCLSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHU1</accession>
    <name>W5NHU1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Denticleless E3 ubiquitin protein ligase homolog (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204342.1">
        <property type="nucleotide sequence ID" value="XM_015348856.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHU1"/>
    <dbReference type="FunCoup" id="W5NHU1">
        <property type="interactions" value="1088"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020200"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020233.1">
        <property type="protein sequence ID" value="ENSLOCP00000020200.1"/>
        <property type="gene ID" value="ENSLOCG00000016359.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695512"/>
    <dbReference type="KEGG" id="loc:102695512"/>
    <dbReference type="CTD" id="51514"/>
    <dbReference type="eggNOG" id="KOG0321">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064210"/>
    <dbReference type="HOGENOM" id="CLU_023407_0_0_1"/>
    <dbReference type="InParanoid" id="W5NHU1"/>
    <dbReference type="OMA" id="PHSQFEK"/>
    <dbReference type="OrthoDB" id="2096344at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016359">
        <property type="expression patterns" value="Expressed in ovary and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002879">
        <property type="entry name" value="Denticleless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000447">
        <property type="entry name" value="Denticleless protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="92"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="355"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="581"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="667" mass="72468" checksum="5DE4E336A88548D5" modified="2014-04-16" version="1">MLFRSIVDRGVLRRRHNGPERVYPLSSLLDCYQCARQDEHISYGDMGVPVPPFGCTFSTAPGLQQILAVANEEGVVRLYDTESRRRPLLKDWLAHTNAVFDIAWVPGEPRLVTASGDQTAKLWDVKTEDLLGTFKGHQCSLKSVAFARQEKAVFCTGGRDGNIMVWDTRCTKKDGCYRQVKQICGAHNKPEKHTPSKPKKKRQVGRGIAPVVDFQQSVTVVLFRDENTLISSGAVDGVIKMWDLRKNYTSHLQDPVPLQVYPYPGTSTRKLGYSGLTLDSTGSSLFANCTDENIYMFNMMGSKVAPVAVFNGHLNASFYIKSSVSPDDQFLASGSSDHNAYIWKISDPTSPPMMLQGHSQEVTSVAWCPTDFTKIASCSDDNTVRIWRLNRGSEGDRFSIGTGNLVGWACPKPPSATKSSGPVSPAESTPTKTSRLDSLGPLASPQAAACAPSGGDLPLPSSTPALSLQTPGSKSHHPGTPRQAAASCTSIRQWMTRTPDSPRPVAQGSPSPRKVLSPCGQSSAPTSERRVKRRLETSAEGVQGCAGDCGCVTELYPAAKRSRGLGVVCFPAQDTDKAGTQELDARQPGEHCSFKSKHPDKENSSPRPADWLSAIGQRLNGGLRSPSPSRSPSATKRQESRTGALLTFSTPCSMKKISFYFQKRTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIC5</accession>
    <name>W5NIC5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome 11 open reading frame 98</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01033523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208442.1">
        <property type="nucleotide sequence ID" value="XM_015352956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIC5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020419.1">
        <property type="protein sequence ID" value="ENSLOCP00000020384.1"/>
        <property type="gene ID" value="ENSLOCG00000016502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107078060"/>
    <dbReference type="KEGG" id="loc:107078060"/>
    <dbReference type="eggNOG" id="ENOG502S62W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002615"/>
    <dbReference type="HOGENOM" id="CLU_162769_0_0_1"/>
    <dbReference type="InParanoid" id="W5NIC5"/>
    <dbReference type="OMA" id="INGPRTE"/>
    <dbReference type="OrthoDB" id="6147870at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016502">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037691">
        <property type="entry name" value="C11orf98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14554:SF1">
        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14554">
        <property type="entry name" value="GENE, 49416-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17719">
        <property type="entry name" value="DUF5564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="135" mass="15202" checksum="47A2D3FA090CC1BB" modified="2014-04-16" version="1">MKSSNGRNLTRGSSMAPGGKINRPKTELKKKLFKRRRVLTKEKRKKHKIVGAIVDQGLITIHHLKKRSSNCRANITLSGKKKRKLMKQLHHMSREKATMEVERAPKGNANSAKGSAKGRKNNATAQDVEMDECMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIQ8</accession>
    <name>W5NIQ8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
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        <property type="molecule type" value="Genomic_DNA"/>
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</entry>
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        <name type="common">Spotted gar</name>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <submittedName>
            <fullName evidence="10">Mitogen-activated protein kinase 8 interacting protein 2</fullName>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
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        <property type="gene ID" value="ENSLOCG00000016754.1"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulation of JNK cascade"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="984"/>
            <end position="1045"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="352"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="488"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="619"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="706"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="761"/>
            <end position="798"/>
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        <location>
            <begin position="56"/>
            <end position="78"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="146"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
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            <end position="185"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="291"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
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        <location>
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            <begin position="467"/>
            <end position="481"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01179"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020709.1"/>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1208" mass="133117" checksum="C445F6E8DD7DDC6C" modified="2014-04-16" version="1">PAHDISLEEFDDEDLSEITDDCGIGLNYDSDPYDKDCLILEKNDLHHPVCSFQDDFQEFEMIDDNDEEDDDEEEEADPEALPSPSASPPASPSVGTLQKNRPTTLNLTHTVSQDSLNNNGSFSPRKASWQESLRHPSSNGRLSPSHSCLEDGTHVNGQCAAAPGAGSQSKGTTPKQAGDTEQTLSPLKPLLYDFEGNRRETLEYGSFGQHKMTTSSDAGAEPSAEVTRPGTSIPVIDEVSQSSDTEVDHDLACDYAKKWGGQSRKSNDTYTVTSESVVDPEIENDITLDGTSKCLSSTAPVPNDAETPISDDELEKDFDIEFTCKESYDLNFKEAGSSSYVEFPPIEPTSFSSYLSSGSRSDAELEESHSHNGASAHGHPSSSSNDTNSPSSDPGIEADLTSKSCKRYIPSSHNIDELSSPGSDSDIEGELEAAFACGGPLVSNMISSISETELDLTSDSSSGRSSHLTNSIEEASSPTSDQELETELEAESGIIGIKASLLLGQPESDKVISPTIDIKAPVQFDDGQALVGLQNVDELACEHTADPDETLPPADQSEDSLSSQLVLKIEPDHSLESFKRSFYLPIGPKLMPEMDDYEGNSEYDSESESEADLSEDSDSPWLLSNLVNKMISEGSYPISCPEDCFKRSGSISDTISPTSDLEADAFNEPLDSQSQKLESNGDMQVQRSEESEAKEKKKNIESKLDSAQTEDTTGLCLYMSNPTNDTISPVFVDRYNKLDKTYVSDNASVANVTKNLKEETIEPNNDLSMEPNEPASPCLSEQLESDKDAGRELSDDTGTLDRIKEAKSSLTLDIPTAQTNRCFNLTYSTDNDEDSFLEGIKKSPYRENVLDSSSATEDNTEDLPPLNSLTTKQVDESLVYDSIKYTLVVDENTTLELVSLKRCTSILSDDSEISTLCDNCDLEVNNDFEVDGIGPEILSSSEDSSPEADVQFSKKFLNVFVNSTSRSSSTESFGLFSCTINGEEREQTHRAVFRFIPRHEDELELDVDDPLFVEVEEDDYWYRGYNMRTGERGIFPAYYAHEVVGQVKEFIGVKRNTAWVERFNVQFLGSVEVPYHQGNGILCAAMQKIATIRKQTVHLRPPAICDLEISLQGVKLIMTLDEYGGNDEFDRCSHFFQMKNISFCGCHPKNNCYFGFITKHPVLNRFACHVFVSQESMRPVAECVGRAFQEYYQEHLEYACPTEDIYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJD2</accession>
    <name>W5NJD2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01023625">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJD2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020741"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020777.1">
        <property type="protein sequence ID" value="ENSLOCP00000020741.1"/>
        <property type="gene ID" value="ENSLOCG00000016779.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXNT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097187"/>
    <dbReference type="HOGENOM" id="CLU_815198_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJD2"/>
    <dbReference type="OMA" id="YGWLRRN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016779">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF36">
        <property type="entry name" value="SI:DKEY-30C15.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="36"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="374" mass="42404" checksum="10AF83636892901E" modified="2014-04-16" version="1">MTPQKTFASSNCTLADSQIWTLSEVYISSLSLSLLGSGSVIVVSMVKKRHLDGQVRPLFQLALADFLAAAVLLSMSVINILTGVTDPDQTSTVLSIYNVCEYAIPLALSFYGTTFLLVMVYAYEAKQAVGGWRESSDGQEYSHRRSRKHTLYLLYFLAWCVPGLCFLTQVVALRNSPAELFPSHPTTNLITFASDTRRNDYHFYCCSCILLVYKENDSSLKVSKAFPEWYTYLMKVFFLAYVLVVITCCIVVYYRVNKWCQDRQGECMFLVEGDGFSRRNMRGIYCTARSVVLVMVVCWTPAFFLVLLSLLIPKQESLFLLYVLQAITVSLQGFLNSIAYGWLRRNFREAALGERLALLAQNPRAFYDESLTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK18</accession>
    <name>W5NK18_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Poly (ADP-ribose) polymerase family, member 12a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034297">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK18"/>
    <dbReference type="FunCoup" id="W5NK18">
        <property type="interactions" value="807"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020977"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021013.1">
        <property type="protein sequence ID" value="ENSLOCP00000020977.1"/>
        <property type="gene ID" value="ENSLOCG00000016964.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSC4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154649"/>
    <dbReference type="HOGENOM" id="CLU_014825_2_1_1"/>
    <dbReference type="InParanoid" id="W5NK18"/>
    <dbReference type="OMA" id="ADMNEIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016964">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056226">
        <property type="entry name" value="WH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057602">
        <property type="entry name" value="Zfn-CCCH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24356">
        <property type="entry name" value="WHD_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23466">
        <property type="entry name" value="WWE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25261">
        <property type="entry name" value="zf-CCCH_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="13">
        <location>
            <begin position="359"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="14">
        <location>
            <begin position="477"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="669" mass="76693" checksum="7357255BAE9E3ECE" modified="2014-04-16" version="1">KMSGLVISNCATKTLCRNQGCLEYRLLKGVVCQSCNVGDRRLFDVLSDSSRFVIVQGREENIPGCDLSPDSIIVAKTRLRLCKSYPECNTCEDLHLCRYFVCGHCRYGQGRNKCRNSHDIHSDHNGAILRAHFLQDLDEAELFQLLLQNDSYLLPEICLHYNKGSGPYGSCTFKERCTNLHVCQHYIQGDCKFGGNCKRFHSFDMNARNILSGRGLSAEKIHILPAIYKNKYRIDSSAQTPAKALTVSIGKKERLRHVSTSSVNEVDRKEICLFFIRKHCSFKDKCVRVHYHLPYKWEILDSDGTTWKYLPNMEEIEKAYCDPENSTRSSYSSLGSPAVNFLTMTCGPAEVRRLSTASSVTKPPHVILTTEWVWYWKNEFGKWIEYGCPEDEQSESSSVTSSVLEKVYLADNESDVPFITGGQQYILSFKDMYQQDRKNTAKREVRRRPRFVSAQDVERKIKRSVSLMTPGSADVSIPSHWVQVPLLDFTHKLVHQSKSGEEYRQIETLFRRSMATSTIHKIQRIQNLYLWNVFQWQKDQMMKRNGGKTVNECFLFHGTEKSLIKAICEQNFDRGISGAHNAYGIGNYFSRDASYFHNNSKPTTGSRIMFVARVLVGDFSQGNSNYLHPPLKEGNSVFYDSCVDSTSNPSVFVIFEKHQIYPEYLIEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKH0</accession>
    <name>W5NKH0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Centromere protein K</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-K/MCM22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001822">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKH0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021129"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021165.1">
        <property type="protein sequence ID" value="ENSLOCP00000021129.1"/>
        <property type="gene ID" value="ENSLOCG00000017098.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVGW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006243"/>
    <dbReference type="HOGENOM" id="CLU_090360_0_0_1"/>
    <dbReference type="InParanoid" id="W5NKH0"/>
    <dbReference type="OMA" id="CVKCNTE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017098">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020993">
        <property type="entry name" value="Centromere_CenpK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14401">
        <property type="entry name" value="CENTROMERE PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14401:SF6">
        <property type="entry name" value="CENTROMERE PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11802">
        <property type="entry name" value="CENP-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="65"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="24553" checksum="111EDA6BDE3C81A1" modified="2014-04-16" version="1">QWKKLEQVQEKISLVGAEALQDTEKQLSISQVLQAKALSAELKKWADREPELLPKNHKVLVAVGMEELQRVNRELEMVLSCCQAKNEKLKHDVKRAVQETKRKIQKIKAYQENLLETFSDFLAEHFPIPQQQANENRKKKVFSLEPTVELISLHDILELLMNKLFESPHDPYITIDETFWPPYTEMLLRHGIALRHPEDSSKIRLEAFHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NL50</accession>
    <name>W5NL50_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-deoxynucleotidase HDDC2</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">HD domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01025945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NL50"/>
    <dbReference type="FunCoup" id="W5NL50">
        <property type="interactions" value="993"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021359"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021396.1">
        <property type="protein sequence ID" value="ENSLOCP00000021359.1"/>
        <property type="gene ID" value="ENSLOCG00000017267.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009937"/>
    <dbReference type="HOGENOM" id="CLU_039453_2_2_1"/>
    <dbReference type="InParanoid" id="W5NL50"/>
    <dbReference type="OMA" id="TWRLCLM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017267">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="15">
        <location>
            <begin position="53"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="223" mass="25416" checksum="2641458BA9382125" modified="2014-04-16" version="1">MFSSGSPLKNMATPSVPADNMKNLLQFMKLIGQLKRTPRTGWVYRNVLNPESVSDHMYRMSLMALTIGDCNINRGRCMQLALVHDMAECIVGDIAPADKVSKEEKHRREKEAMKHLTGLLSEDMKKEIYDLWEEYEYQASPEAKLVKELDRFEMILQAHEYETLENKPGRLQDFFDSTNGKFQHPELLELVKSLNEERNMQLAATGSSPDRGASLPGDRVSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLR0</accession>
    <name>W5NLR0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003298">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLR0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021569"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021606.1">
        <property type="protein sequence ID" value="ENSLOCP00000021569.1"/>
        <property type="gene ID" value="ENSLOCG00000017464.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVH7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234640"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NLR0"/>
    <dbReference type="OMA" id="MEIKYFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF345">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="316" mass="36257" checksum="E5979240F1FA80A4" modified="2014-04-16" version="1">MQNLTPVTVFLFTAYEAKGNIKYLYFTLTLLTYILIVFVNTVLITTIARERKLHEPMYIFICNLSLNGVYGSTAFYPIILANLLSEAPSISRTGCLTQIFFIHTCGSFEYSILALMAYDRYVSICHPLRYNCIMTPSVVTQLIVFVYMYPIFIMLIHLVLTVRLPLCGFIIEKVYCDNWSVVRLACSDVSVNNIFGLLVTGLLMGVPVAVIFYSYVRILAVCLRSTKEARAKALQTCTPHLLSFTNYSIATFFEVLQHRFEFSNIPHVVRIMMSINPVLLPPLLNPIIYGVKLQEIRQRTMKMFYRRKTNYIGESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMG2</accession>
    <name>W5NMG2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division cycle associated 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031900">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206525.1">
        <property type="nucleotide sequence ID" value="XM_015351039.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015351040.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206527.1">
        <property type="nucleotide sequence ID" value="XM_015351041.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015351042.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069049452.1">
        <property type="nucleotide sequence ID" value="XM_069193351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMG2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021821"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021858.1">
        <property type="protein sequence ID" value="ENSLOCP00000021821.1"/>
        <property type="gene ID" value="ENSLOCG00000017716.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682170"/>
    <dbReference type="CTD" id="55038"/>
    <dbReference type="eggNOG" id="ENOG502QWNU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063867"/>
    <dbReference type="HOGENOM" id="CLU_065586_1_0_1"/>
    <dbReference type="InParanoid" id="W5NMG2"/>
    <dbReference type="OMA" id="SSVWEMD"/>
    <dbReference type="OrthoDB" id="6083860at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017716">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0003713">
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    </dbReference>
    <dbReference type="InterPro" id="IPR052262">
        <property type="entry name" value="E2F-SERTA_domain_protein"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277">
        <property type="entry name" value="CELL DIVISION CYCLE ASSOCIATED PROTEIN 4/SERTA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="2">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="263" mass="29029" checksum="2B0D743A36C4E70B" modified="2014-04-16" version="1">MFPKGTKRKYCDGDEIVDESPASLKVVSSYDLQRQSLLDMSLIKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGSWQVMTEALQASQNSADRLVATEILCRPSPKEQDEEKSFNVGGYDACHTLGGNPGEVVDETLCTVSPEAASAYLPSAIATWEKEDQKENRPEEEDAKHAEQVFGSFEIKNPAPSPDPALEELFSDVDTSYYDLDTMLTGMQSSPKMGPYDLLESLSSHSPSSISSNSSCRPDLNELDHIMEIIVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMV0</accession>
    <name>W5NMV0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cannabinoid receptor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_006631581.2">
        <property type="nucleotide sequence ID" value="XM_006631518.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204387.2">
        <property type="nucleotide sequence ID" value="XM_015348901.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMV0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021959"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021998.1">
        <property type="protein sequence ID" value="ENSLOCP00000021959.1"/>
        <property type="gene ID" value="ENSLOCG00000017856.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688038"/>
    <dbReference type="KEGG" id="loc:102688038"/>
    <dbReference type="KEGG" id="loc:102688950"/>
    <dbReference type="CTD" id="1269"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282530"/>
    <dbReference type="HOGENOM" id="CLU_009579_7_0_1"/>
    <dbReference type="InParanoid" id="W5NMV0"/>
    <dbReference type="OMA" id="PYVDHHY"/>
    <dbReference type="OrthoDB" id="10011551at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017856">
        <property type="expression patterns" value="Expressed in mesonephros and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:cannabinoid receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cnbnoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CANNABINOIDR"/>
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    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="62"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="362" mass="41998" checksum="6BC86FD22E98F96E" modified="2014-04-16" version="1">MEEVDNCSTVWKSNISVCCPFYVNSPEDGPCPGLQCYMVLEKREKTVISTICFFAGPLTFLENVLVLCLIFFTPALRRKPSYLFISSLALADVFASLFFTISFLDFHLFERTDNAEVYLFKLGGVTLSFTSSVGSLLLTALDRYICMHRPSEYKTILTRRRALACLTALWMTTIFIAFLPPLGWNCKTMDRPCSQLFPYVDHSYLACWIILVMFLLVLIIYAYIHILWKAHKHVVYMAKHSIRPGHVRMRMDIRLARMFSLILAILVTCWFPVLAFMIVDFFHCLSREQKRAFAFWSTLCLVNSSVNPLIYALRSRDMRRVMLKVLSWCRGQRSYMEGSQESDMQQKPSNQETISERVEKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNF2</accession>
    <name>W5NNF2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Actin-3-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNF2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022161"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022202.1">
        <property type="protein sequence ID" value="ENSLOCP00000022161.1"/>
        <property type="gene ID" value="ENSLOCG00000018060.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162158"/>
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    <dbReference type="InParanoid" id="W5NNF2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018060">
        <property type="expression patterns" value="Expressed in testis and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ACTIN"/>
    </dbReference>
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        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="392" mass="43715" checksum="981FD9B8043A963C" modified="2014-04-16" version="1">GESETSAAAKTAGDQQEDCRRQIKTQNSGTKLTGPVILDLGTGYLKAGFAGEKKPLCVISSVIKKVREFPGNCKQSFAGKLLEKPEHDLIYPLKNGLVDDWDTLEELWKHVSEKELGIATEDHPVLVSEHPLTPTRSREKMAELMFETFSVPAVHMAYQPALLLYSYGKTSGLVLESGHGGSYVAAVYNGCHRPTTACVVDYGGQTLNAHLLRLLNESGQGIQDHSLNIVEEIKEKCCYVARDIDYELYLPESRYKVEYELPDGRIIFVGKERFTCPEALFHPNKMGSTEPGLPTLMMNCINKQDRRTRGELLRNIVIGGGSTMFMGFPKRLTQELGRLSLNSDVLIEAGFQRKHTVWIGGSILASLRSFHPLWVQKKEYDEKGVSALHRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNF7</accession>
    <name>W5NNF7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01014334">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNF7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022166"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022207.1">
        <property type="protein sequence ID" value="ENSLOCP00000022166.1"/>
        <property type="gene ID" value="ENSLOCG00000018065.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SNIT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168880"/>
    <dbReference type="HOGENOM" id="CLU_2711437_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNF7"/>
    <dbReference type="OMA" id="AGICNHA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018065">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14965:SF9">
        <property type="entry name" value="APOPTOSIS FACILITATOR BCL-2-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14965">
        <property type="entry name" value="SI:CH73-248E21.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="74" mass="8386" checksum="60879F954545D1C2" modified="2014-04-16" version="1">MNYSSFRELADQYVETETPSRRPQGNPPLELVKFAFALDFTAKVAGICNHTVSCVMGFGNQYLQDRFTQYQNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LV87</accession>
    <name>W5LV87_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LV87"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000044"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000044.1">
        <property type="protein sequence ID" value="ENSLOCP00000000044.1"/>
        <property type="gene ID" value="ENSLOCG00000000035.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153956"/>
    <dbReference type="HOGENOM" id="CLU_000134_11_1_1"/>
    <dbReference type="InParanoid" id="W5LV87"/>
    <dbReference type="OMA" id="QYDCGEH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000035">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000453">
        <property type="entry name" value="Ankyrin repeat domain 1a (cardiac muscle)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000111">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF7">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="315" mass="35497" checksum="A80A443C1DF73426" modified="2014-04-16" version="1">MGMLRVEELVTGKKCEGKETGEGLTEEFAEGEYESVVTREKQDDLKSHEGLSLIQNDIAKREKKEKEIKIYKAHELKLETLDDLQSIVKLKKKKRVKRVPALNKEPEPEIIPDTVDVATFLKAAVENKLPVIDKYLADGGDPNVYDKFERTALHRACSEGHVEVVKRLLDAGALIEKKDKLESTAVHWVCRGGCLPALEILLNKNGNLNARDKLRSTPLHVAVRTGHYECAEHLIACGADINARDLEGDTPMHDAVRLNRYRLIRLLLIYGANLNVKNCEGKTPLESVLQWQSGTKHILNSHTEEPYRKAHSNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWS1</accession>
    <name>W5LWS1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWS1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000578"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000579.1">
        <property type="protein sequence ID" value="ENSLOCP00000000578.1"/>
        <property type="gene ID" value="ENSLOCG00000000526.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286938"/>
    <dbReference type="HOGENOM" id="CLU_077975_1_2_1"/>
    <dbReference type="InParanoid" id="W5LWS1"/>
    <dbReference type="OMA" id="PEWVAHI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000526">
        <property type="expression patterns" value="Expressed in bone element and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002762">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="148"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="245" mass="26774" checksum="D21CF9FD7E5A69E4" modified="2014-04-16" version="1">VLTQSDPQVKKPGESLTLSCQGSGQDENGYGFSDSYMNWIRQTPGKGLEWLADISDDGDSTSYAKSVKGRFTISRDNSKSTLYLQMSSLKTEDSALYYCARDTMIKKTWSSMSLSGSMGSGTSIQISSGLNLHCVQLTQSASEIGKPGQSLLLSCQVSGYPLTDSSYATGWIRQPPGKGLEWLGHYYTYGNSYAPSVQGRFIISTDSGSTVYLQKTSLKAEDSGVYYCARDSQFFLISYLSCLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXP2</accession>
    <name>W5LXP2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LXP2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000899"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000903.1">
        <property type="protein sequence ID" value="ENSLOCP00000000899.1"/>
        <property type="gene ID" value="ENSLOCG00000000809.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074977"/>
    <dbReference type="HOGENOM" id="CLU_2757123_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXP2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000809">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="70" mass="7764" checksum="FB289E551E1BF8F5" modified="2014-04-16" version="1">MDGIYTRMHSRESVSLGLSTFMIDLNQMASALNDSTGSSLVLIDEFGKGTNTVRRRRRRKIGWGASHCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXP4</accession>
    <name>W5LXP4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LXP4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000901"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000905.1">
        <property type="protein sequence ID" value="ENSLOCP00000000901.1"/>
        <property type="gene ID" value="ENSLOCG00000000813.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2078392_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXP4"/>
    <dbReference type="OMA" id="QNEDAYS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="12937" checksum="D628D555EB794F8B" modified="2014-04-16" version="1">SMSSVGLSALYYNPRESGSYGGVGSLVRAARSRGVSGSSRSRVAKWLSGQAAYTLHKPARVHFKRNRTLWQADLVDMREYSEFNDGFCYILTCIDMLSKYAWAVPLKTKSGGAVTGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXZ1</accession>
    <name>W5LXZ1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inosine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="W5LXZ1"/>
    <dbReference type="FunCoup" id="W5LXZ1">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000000998"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001002.1">
        <property type="protein sequence ID" value="ENSLOCP00000000998.1"/>
        <property type="gene ID" value="ENSLOCG00000000895.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="HOGENOM" id="CLU_054456_1_3_1"/>
    <dbReference type="InParanoid" id="W5LXZ1"/>
    <dbReference type="OMA" id="MSIVWES"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000895">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF12">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="15">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="233" mass="25649" checksum="EE77C71932E48C06" modified="2014-04-16" version="1">VHGHAGRLIFGTLNGKPCVCMQGRFHLYEGYPIWKVTFPIRIFKLLGVQTVILTNAAGGLNQDYKVGDVMLIKDHLNLPGFAGNNPLVGPNEERFGVRFPCMSDAYDRDLLKAAQVVGAELGYSDFMKEGVYCVLGGPSFETIAECRMLNRLGADAVGMSTVHEVIVARHCGLRVLGLSLITNKAVMDYESEERANHEEVLQTGKQRAEQLEKLVSTLVARVELNNNYCGFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ94</accession>
    <name>W5LZ94_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Bone morphogenetic protein 8a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009362">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009364">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ94"/>
    <dbReference type="FunCoup" id="W5LZ94">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001451"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001456.1">
        <property type="protein sequence ID" value="ENSLOCP00000001451.1"/>
        <property type="gene ID" value="ENSLOCG00000001276.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155272"/>
    <dbReference type="HOGENOM" id="CLU_020515_4_1_1"/>
    <dbReference type="InParanoid" id="W5LZ94"/>
    <dbReference type="OMA" id="DRDIFHQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001276">
        <property type="expression patterns" value="Expressed in zone of skin and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0002230">
        <property type="term" value="P:positive regulation of defense response to virus by host"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="FunFam" id="2.60.120.970:FF:000017">
        <property type="entry name" value="Bone morphogenetic protein 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000020">
        <property type="entry name" value="bone morphogenetic protein 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF119">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865369" evidence="14">
        <location>
            <begin position="37"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="15">
        <location>
            <begin position="305"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="436" mass="49167" checksum="25DEA4B1F2D63E4E" modified="2014-04-16" version="1">SSGRRRRNSAPHRQLFPLSLPLLLLWLLSLWGSAEGREPHSGFLLRRLSVRERKEVQREILSILGLPGRPRPHPPLRPPSAAPLFMLDLYHTVSAGAEEGEYLGLGLGSAGLHLGHAALPTLSTHIPPLGTMVNEADTVMSFVNVVEQERDFFQPRPYWKEFRFDLTPLPQGETVTAAEFRVYKTLSLGPRGNRTLHISVYEIVKQNRHREPELVLLDLQSVPGGQEGWLVFDVTAATNRWQQNPHSNHGLRLYAETEEDRSLSPGWVGLVGRRGPRSKQPFMVTFFRASHTPTRAPRAARTSNRKKKPKYDLPHPNRLPGIFDHNHINSGRQACKRHELYVSFSDLGWKDWVLAPSGYSAFYCDGECMYPLGSCMNATNHAMIQLVVHLLKPDEVPKACCAPTKLSPISVLFYDDNNNVILKKHRNMVVKTCGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZZ9</accession>
    <name>W5LZZ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Ssemaphorin 4F</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZZ9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001706"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001711.1">
        <property type="protein sequence ID" value="ENSLOCP00000001706.1"/>
        <property type="gene ID" value="ENSLOCG00000001476.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3611">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159592"/>
    <dbReference type="InParanoid" id="W5LZZ9"/>
    <dbReference type="OMA" id="APLAKCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001476">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045791">
        <property type="entry name" value="Sema4F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF72">
        <property type="entry name" value="SEMAPHORIN-4F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19428">
        <property type="entry name" value="Sema4F_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="666"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="17">
        <location>
            <begin position="38"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="528"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="698"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="750"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="783" mass="84926" checksum="43FC5683DB11637A" modified="2014-04-16" version="1">NPGPVSKVFSKRRALGGTPHPLSPLLLVWGPAGCPRVIKSLARGSLCAELGTALVRWRLFSVSNYSACLLDPSSGILYLGARDTILALDTRDFTKWRTISWEVPKDKRDTCINKGKTEADCHNYVRLLQFLSAEQIYVCGTYAFDPQCTFLELQENGEVRMESGKGKCPFEPAQHYTAVMADGLLYTATTSNFLSTAFDISRATGPEVERIRTETSPSWLSDPEFVSSVFVPEKKDSPTGDDDKIYFFFTEVANEYNFYTRVRVPRVARVCKGDVGGSKTLQKRWTTFLKALLVCEDSQSGRRYDILKDVYTLEHRPGDTGSTHFYGLFTSQWDSEEVSAVCVYGLQDIGAVMSGSYKKKNCDSWSQQEPPPHPRPGQCITEALRAEGYESSLKLPDKVLTYVRDHPLMEIPATAHPLLVRWGVTYTRLAVRLAPGPPGPPATALLHLGTDRGELHRVSVRGLDDTLLQETPLFDTGEPVTRILLTEDWVLVGSPSSLVKLQGCGQYSFCEGCAQAGCGWDPESGTCTDPRDKKGGPGRAVRSVGSGSAPGLTPPLSPLPSPPAGRCSPLPQEVRAVKGQRLLLPCVHLSQAGCHWVHPGDRHTRQRHAHLEMVVSDGSVGEYRCECAEGGASGSEPTLCTRAAFHVVLGAPSTDAVEGEPRCRTFGIGLLCVFLGAALGAALCFLLLRVRRAAARAPQSEKDAAGKAMYADTPNSPSSPSLLSEGVPLTKKNGALNGHTYANVNQGCEGRPESEAGERQGEENPRVLQLAGAPLASCEESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M012</accession>
    <name>W5M012_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Proteoglycan 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01034680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034681">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M012"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001719"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001724.1">
        <property type="protein sequence ID" value="ENSLOCP00000001719.1"/>
        <property type="gene ID" value="ENSLOCG00000001502.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063751"/>
    <dbReference type="HOGENOM" id="CLU_008106_0_0_1"/>
    <dbReference type="InParanoid" id="W5M012"/>
    <dbReference type="OMA" id="PTTQHET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001502">
        <property type="expression patterns" value="Expressed in bone element and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051298">
        <property type="entry name" value="Heme_transport/Cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020436">
        <property type="entry name" value="SMB_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917">
        <property type="entry name" value="HEMOPEXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22917:SF1">
        <property type="entry name" value="PROTEOGLYCAN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00022">
        <property type="entry name" value="SOMATOMEDINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SMB" evidence="9">
        <location>
            <begin position="23"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="9">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="7">
        <location>
            <begin position="319"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="507" mass="55965" checksum="1598AF090B2D31D4" modified="2014-04-16" version="1">QVYSFKTVVVAFLTKPSFVTLGTTSSCNDRCGESYYRGDICHCDYDCLLHKECCSDYEAFCTTVDSCKGRCGETFQRGKQCYCDVECSRYNQCCPDYRKHCTIEAKIPDGTVSTETTPPAKVQKLLDSTLTNPFVLNDEVKPSAVTAQTPVATEDLAKSTVSPVPDDEVKLPAVTPQSPVGTEDLAKSTMSPEPENIASQTTAHNGVPSSVSVEGIKDMFTLTHTSVHTSLPEPSERHIPVPSTSSGTVTIQSTVPSKHPSDSVGENNDTNICSGRPANALTTLQNGTMIVFRGHFFWTFGSNGALGHPRRITEEWGIPSPIDTVFTRCNCQGKTFIFKANEYWRFDNGVMDRGYPKLISKGFGGMSGRITAALSVPAFGKRREAVYFFKRGGLVQKYSYKQEPSQSCGKPQTQYRIYSLHSRITRQAGPVYLGKEINIKLTWKGFPTSVTSAISLPNSKKPDGYDYFVFSRAKYYNIIINSDQPAVTPLLKSVTHKDTAKDWYHCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1W3</accession>
    <name>W5M1W3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49092"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01021706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021707">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021708">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1W3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002371"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002376.1">
        <property type="protein sequence ID" value="ENSLOCP00000002371.1"/>
        <property type="gene ID" value="ENSLOCG00000001986.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102694049"/>
    <dbReference type="eggNOG" id="KOG2968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156763"/>
    <dbReference type="InParanoid" id="W5M1W3"/>
    <dbReference type="OMA" id="VSKAHFY"/>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001986">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000012">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000022">
        <property type="entry name" value="Patatin like phospholipase domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF23">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="174"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="477"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="609"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="21">
        <location>
            <begin position="948"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="340"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="17">
        <location>
            <begin position="952"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="17">
        <location>
            <begin position="979"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="17">
        <location>
            <begin position="1101"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="981"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1251" mass="138999" checksum="03FDE47860AE7601" modified="2014-04-16" version="1">MNEDDNCSIYPGALPLPSGSEIRARVQQFVEERMQTTMLTGVLIGAVVAISLIGIMVLFVYRRYKQTREQQAGVPRYRFRKRDKVLFYGRKIMRKVQTLSSAPSSGGSVSRQRSRKRPKVLALARRILRIRREPPTLQPKEPPPSLLEADLTEFDVQNSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFMELQEGEGLFRPGDTDDSIYVVQDGRLELCVQESDGTDPVVKEVLPGDSVHSLLSILDVITGYPAPYKTVSARAALRSTILRLPATAFQSVFNKYPETLVRVIQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLVSVFSVIGEGSPGRTSRKPHPHTPPEEEPGLDKCPQGAAERSSDTDAVRSFAADSASAFRKSRSLSVESTGGAVSSSTDLNMAYERARVTIEDAPPSPVMHKSILKKSVTMQPAPSAVIHYTDSGGRSDQVPPSKVGAIFQAAKKDLLRLIKLNDHSLLEGRVTLHQVKAGTVVARQGDQDVSIRFVISGLLHVYQRMIDREEDTCLFLTHPGELVGQLAVLTGEPLIFTVRAHRDCSFLSVSKTHFYEIMRAEPSVVLSVAHTVVKRMSSFVRQIDFALDWMALEAGRAVYRQGDKSDSTFIVLSGRLRSVILKDDGKKELTGEYGRGDLIGVVEALTHQPRATTVHAVRDSELAKLPEGALSSIKRRYPQVVTRLIHLLGQKILGNLQQVNGPLAARSLTLNTPGSKWDVGNPVSNLSTVSILPVCEDVPLTAFSLELQHALSAIGPTLLLTSDIIKQRLGSAALDSVHEYRLSSWLGQQEDIHRIVLYQSDPSLTPWTQRCIRQADCILIVGLGEQEPTVGELERMLESSAVRAQKQLVLLHREDGPPPSGTVEWLNMRSWCSRHLHLSCPRRVFSKRSLPKLREMYQRVFQKSPDRHSHFSRLARVLTGNAIALVLGGGGARGCSQVGIVRALSEAGIPIDLVGGTSIGALMGALYAEERNHNRMRIRAREWAMEMTSVFKKVLDLTYPVTSMFTGAAFNSGIYSVFKDKQIEDLWIPYFNITTDITASAMRIHTDGSLWRYVRASMSLSGYLPPLCDPKDGHLLMDGGYINNLPADVARSLGAKVVIAIDVGSQDETHLTNYGDSLSGWWLLWKRWNPLAERVKVLNMAEIQTRLAYVCCVRQLESVKNSDYCEYIRPPINRYGTLEFGKFDEIAVSPRGLLPHVLWPVGLSASPSPPSGKTLRRRELCGHNSRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M206</accession>
    <name>W5M206_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01021695">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M206"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002419.1">
        <property type="protein sequence ID" value="ENSLOCP00000002414.1"/>
        <property type="gene ID" value="ENSLOCG00000002080.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064359"/>
    <dbReference type="HOGENOM" id="CLU_021496_2_0_1"/>
    <dbReference type="InParanoid" id="W5M206"/>
    <dbReference type="OMA" id="WIAFNDI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002080">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="44439" checksum="E6C0599591C9B4EA" modified="2014-04-16" version="1">AIVRVENAKAFVISAYFDDRFSPGMVRIIGIVYRAGYPNFYCHFSDTKQILTIKAKVLVHNEHFNFPYGTADFLCKTPAGAGDFPFVHVGVSPQMDPPGSFLKIRNTVKNISADFPSKFSVCISTMFGNYSNVLQVVQAVEMYRILGAHRVIFYKSSCSALMQSVLDYYTTLGVVEVIPWNIHLHLKASAGWQPHVHPGDLHYYGQVTALNDCIYYNMYSSHYVVLNDIDEVIVPNLHQDWGEMMRFLSRRHLGVNAFLFENAVFPYSVFGDNGTFDLERWASIPGVNILRHVHREPERFLTFNPRKLIVNPRAMIWTSVHNVPWLSGDWLKVSGTVARLHHYRTPEQPHLRKEDLIRDTTLWRYKASLTRNVDHVLQEVLEIPSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2I1</accession>
    <name>W5M2I1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Retinoblastoma binding protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009451">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631293.1">
        <property type="nucleotide sequence ID" value="XM_006631230.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2I1"/>
    <dbReference type="FunCoup" id="W5M2I1">
        <property type="interactions" value="2183"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002589"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002595.1">
        <property type="protein sequence ID" value="ENSLOCP00000002589.1"/>
        <property type="gene ID" value="ENSLOCG00000002213.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682231"/>
    <dbReference type="KEGG" id="loc:102682231"/>
    <dbReference type="CTD" id="5928"/>
    <dbReference type="eggNOG" id="KOG0264">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153375"/>
    <dbReference type="HOGENOM" id="CLU_020445_3_1_1"/>
    <dbReference type="InParanoid" id="W5M2I1"/>
    <dbReference type="OMA" id="PHEEGCL"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002213">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000021">
        <property type="entry name" value="histone-binding protein RBBP4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="12">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="173"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="223"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="313"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="424" mass="47611" checksum="4DDE13CC1760A097" modified="2014-04-16" version="1">MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSVHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPTSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGYLLSASDDHTICLWDISTVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHAVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGTADAEVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3T4</accession>
    <name>W5M3T4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="EMBL" id="AHAT01028599">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221417.1">
        <property type="nucleotide sequence ID" value="XM_015365931.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3T4"/>
    <dbReference type="FunCoup" id="W5M3T4">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003042"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003048.1">
        <property type="protein sequence ID" value="ENSLOCP00000003042.1"/>
        <property type="gene ID" value="ENSLOCG00000002575.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686577"/>
    <dbReference type="KEGG" id="loc:102686577"/>
    <dbReference type="CTD" id="352941"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158044"/>
    <dbReference type="HOGENOM" id="CLU_013779_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3T4"/>
    <dbReference type="OMA" id="CIAHAAV"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002575">
        <property type="expression patterns" value="Expressed in muscle tissue and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010875">
        <property type="term" value="P:positive regulation of cholesterol efflux"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF1">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="102"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="307"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="652"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="727"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="657"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="786" mass="85788" checksum="E2F401A2D57E2216" modified="2014-04-16" version="1">MPNSTAKRFKPTKYIPVSTAATLLVGSTTLFFVFTCPWLTQAISPAVPLYNGVVFLFVLANFSMATFMDPGVFPRADEDEDKDDDFRAPLYKNVEIKGIQVRMKWCATCHFYRPPRCSHCSVCDNCVEDFDHHCPWVNNCIGRRNYRYFFLFLLSLSTHMVGVFSFGLLFVLHHLERLGALHTTVTLVVMCVAGLFFIPVIGLTTFHMVLVARGRTTNEQVTGKFRGGVNPFTRGCRGNVEHVLCSPLAPRYIADPREKPQVCIKPPFLHPSLTDRQITVKISDNGIHANIHRTKSKSSLDALEDLQMDSQPPLPPKADPSKYTELKSQLTSSEESTLSTQPLNPSTPAMYKYRPSFSTMPKVHYHPTGEKIVMQDDREAAAIVEESGRGHDYRSEPNLDLPEYRNPSLHRTFQSSPLQLDSFAASSRSLSLKHAHRRGGDKAALHPLRSEGATSTPYKSIFSPGGALSSRNGSLSYDSLLNPALSPAASECLAHPGVAPGGYHSPYLSAKMCHVRGSELQRQLPPTYSPILGGGGGGRRSPHPREPSPVRYDNLSKTIMASIQERKELEEREKLLHLHAQAQAQAQAADSGVYDAPSGYSLQPGACFPDDPRGHPPAGPFGSRDDLMGSVSAFGARNPLLRSSATSLGSAALSRAPRTSTTSLHADLANNNVQGRPSEAPYRSPAHQVPHSPLVLPRSPSYSHQKPLSFINALEMTDSPPLGGPRDDGHVKQAHSKINGQPKGLTRADCRLGTPSGSQSTPVSPGRHTSVKKVSGVGGTTYEISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M486</accession>
    <name>W5M486_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01038722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01038723">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M486"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003194"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003201.1">
        <property type="protein sequence ID" value="ENSLOCP00000003194.1"/>
        <property type="gene ID" value="ENSLOCG00000002719.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286950"/>
    <dbReference type="HOGENOM" id="CLU_1767441_0_0_1"/>
    <dbReference type="InParanoid" id="W5M486"/>
    <dbReference type="OMA" id="MICINGH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="72"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="147" mass="16579" checksum="4E2777ABF037B9A4" modified="2014-04-16" version="1">MAEQFRRTRLDRADGGAAGPGEVPCDVCAAEKLRAVKSCLVCLASYCDEHVQPHYQGAAFRTHRLTEPVSHLKHKLCKRHERLLELFCTTDRTCICQFCAESDHRTHSIVPLEEECRARKARRLNQLTSHLPSLGYANCSWSGTESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5G0</accession>
    <name>W5M5G0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5G0"/>
    <dbReference type="FunCoup" id="W5M5G0">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003618"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003625.1">
        <property type="protein sequence ID" value="ENSLOCP00000003618.1"/>
        <property type="gene ID" value="ENSLOCG00000003047.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162782"/>
    <dbReference type="HOGENOM" id="CLU_005389_5_1_1"/>
    <dbReference type="InParanoid" id="W5M5G0"/>
    <dbReference type="OMA" id="CEPGEGP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003047">
        <property type="expression patterns" value="Expressed in ovary and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000067">
        <property type="entry name" value="Olfactory receptor C family, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000519">
        <property type="entry name" value="Vomeronasal 2 receptor, a18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000125">
        <property type="entry name" value="Vomeronasal 2, receptor 88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF418">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCQ19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="607"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="677"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="762"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="801"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="833"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="607"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="900" mass="100679" checksum="82D246CDDECBB6DD" modified="2014-04-16" version="1">KQGRLIRTKTMQFILYIFVALGFIGQVDTVVPPCRLQEKFDLYGLYKEGDIILGGISEVHFITVYPEMSFTSKPEPLSCERFDFTGFQWAQTMAFAIEEINRDPALLPNITLGYRLYDNCVNLAVALRAATALISGEDDSVTDYSCRGTPPVLAIVGDPVSTYSIAMSRILGLFRIPMVSYYATCSCLSNRKEFPSFFRTIPSDTFQVKAMVQIFKHYGWTWVGVIVSNDDYGLYAAKTFIEEVNSFGCIAFSEILPRNNDKSEILKIVRTIKQSTAKVIIVFSTAAFLIPLTEEIVQQKIRGRQWIASEAWASSPVFHTKELLPYLGGTIGITVRRGEIPGLEQFLLQIRPVFDSRNNLVKLFWENMFDCKFQENTNNHNLSNWKGSKICTGSEDIGKTRTAYSDVSELRGSYNVYKAVYALAHSLDNLMSCEPGKGPFQNNSCADITTVQPWQLLHYLARVNFTTHYGDRVSFDENGDALAIYDVMNWQRADDGSVKTVTIGLFDESAPAGQQLTLNEDKIFWNFESKRPPRSVCSESCQPGTRKATRKGEPLCCFDCVPCAEGDISNHTDAFECLKCPPDFWSNPSRDQCVPKEIEFLSYEEPLGISLTTVSVFGAFISAGVLAVFIYYRNTPVVRANNSELSFLLLLSLILCFLCSLLFIGQPLHLTCMLRHVVFGISFVLCISCILVKTIVVIMAFRATLPDNNVMKWFGAAQQRGTVFVFTVIQALICIIWLSTASPVPFKNTQYQNSKIIFECNVGSITGFSFLLGYIGLLASVCFLLAFLARNLPDNFNEAKFITFSMLIFCAVWIAFIPAYVSSPGKYSDAVEIFAILASSFGLLIAIFVPKCYIILLHPEKNTKKSLMGRAAPKTNLRSITCSTDKTEMNATLSRHGLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5J3</accession>
    <name>W5M5J3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sushi domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005094">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5J3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003651"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003658.1">
        <property type="protein sequence ID" value="ENSLOCP00000003651.1"/>
        <property type="gene ID" value="ENSLOCG00000003095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687922"/>
    <dbReference type="KEGG" id="loc:102687922"/>
    <dbReference type="CTD" id="56241"/>
    <dbReference type="eggNOG" id="KOG4291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110943"/>
    <dbReference type="HOGENOM" id="CLU_019295_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5J3"/>
    <dbReference type="OMA" id="FGADWAI"/>
    <dbReference type="OrthoDB" id="6051552at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003095">
        <property type="expression patterns" value="Expressed in intestine and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF63">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867655" evidence="8">
        <location>
            <begin position="30"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="785"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="11">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="9">
        <location>
            <begin position="285"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="12">
        <location>
            <begin position="445"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="724"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="830" mass="92035" checksum="98C8732014F827A9" modified="2014-04-16" version="1">MKGKVGINGILLFEIIIILISCVPERTDAQTCENNCASKNVGNCSCHVTCEPLGTCCGDYRNFCLEVSPHSGTLLGGTDITILKSSFEPSSAIRCRFNTDVETTGYVDSERNGHCISPLLYETGWIPFEVSTDNGVNYNRHGTWLSVHHSKMDPRFKILLLNATKWQYYGTPNTGGSLAMVWNTSFVSADAVNVELWGYREKGEPYSSSWEPEWSFLYTLGKAVPNNGSFGFVPSPAKKPFSDWEVGAIRVSPSTQPEGAWNINAMWSGVHALAWHLEEEFRKDSAAWALDKCLRWHETELKLPNFLSEIADCPCTLAQARADTGRFHTDYGCDIEKGSVCTYHPGSVHCVRAIQASPKYAAGQQCCYDSTGAQVLTADSIGGSTPDRGHDWGSPPFKKPPRVPGVSHWLYDVISFYYCCLWSDNCSYYFTHRPSSDCKTYKTPKPGIVFGDPHVITFDGSSYTFNGRGEYYLLHSTHKQLTIQGRTKPVAFENGTLAKATGLSAVAMQEDNSDIIEIRTTDRQDHLEVLRNQQVLSFSEQSWMDLKGVFLYSAVPQNVTVMFPSGAGVELRGRGGVMSASVLLPEEFRNHTHGLLGLMNDSPEDDFVFKNGTILPAERRSPEDLFHFGANWAITNESSLFTYDNQYLLDNYYFAEKHDSSFIPAYTVTVPPEDPLFADMVRLCNENEFCKYDTLTAQSLKMGNATRISFQSHMSLVKDLEPVISCGWLPPPNNGKKEGTTYLAGATVKFSCDDGHVLSGSAERTCQDDGNWSGDTTHCVSDNTLGIVLGSVFGAITLITMIVIIALHSRKQKRNARTTKQVVGELSMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5T1</accession>
    <name>W5M5T1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS15m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S15, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5T1"/>
    <dbReference type="FunCoup" id="W5M5T1">
        <property type="interactions" value="600"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003739"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003746.1">
        <property type="protein sequence ID" value="ENSLOCP00000003739.1"/>
        <property type="gene ID" value="ENSLOCG00000003165.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001737"/>
    <dbReference type="HOGENOM" id="CLU_094627_1_1_1"/>
    <dbReference type="InParanoid" id="W5M5T1"/>
    <dbReference type="OMA" id="EHLHMHP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003165">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000015">
        <property type="entry name" value="Mitochondrial ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
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        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46685">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S15, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46685:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
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        <property type="entry name" value="Ribosomal_S15"/>
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    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
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    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
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            <begin position="192"/>
            <end position="214"/>
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        <location>
            <begin position="54"/>
            <end position="85"/>
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            <begin position="192"/>
            <end position="208"/>
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            <dbReference type="ARBA" id="ARBA00004173"/>
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            <dbReference type="ARBA" id="ARBA00008434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00035249"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00035528"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="214" mass="24987" checksum="FB5B756A1B3ACFD7" modified="2014-04-16" version="1">SPDSSSVVQAVRNYVRAVRKRQEVPSQLDDLPATMLKIDYAAVQMAEKADDTVKKLLTLELASHKEKLRIKKEQLMAKVKRNESDRGSTEVQVAVLTAKIRNYQEHLQYHTKDKANKRRMLMAIDRRKKLLKYLRRTRYDLFENVCQQLGITYTFPPEYYRRVTRRWAAKKAFCIKVFNEVQKQKAAEKKRQREAATLKEESADKQKGVDGSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5X1</accession>
    <name>W5M5X1_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Protein N-terminal asparagine amidohydrolase-like</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="NTAN1"/>
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        <property type="entry name" value="N-TERMINAL ASPARAGINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN N-TERMINAL ASPARAGINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF14736">
        <property type="entry name" value="N_Asn_amidohyd"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="307" mass="35246" checksum="8AB499FF745F244C" modified="2014-04-16" version="1">MPLCVKHKLVEDFETIKEFWAKYGEDLQPEAEKFKQKPEVIEQPDYLLYVSQREYATIDLCKGPVTRIGSDEATTCHIIVLHCPGTGKVSLAHIDGSKVGVGVKFMVEEMKKQCKDFSHSLDLYMAGGFIDERGISHDNTMKLLREFMKQDCQIELQLACVSEMNDVVREGNHWPVVSGVGVKMDGNSFHIYRATFPEKVPDITLRGARLTVDMMMRRVYDTETDRLIVGPYNFYEHMLKTDDDSRDLTDGEIRRGFSTSPLVERPEFVQMIKDHYKYLADHLKLQITPSLPIGYVMNHGKWIPCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M679</accession>
    <name>W5M679_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TCDD-inducible poly(ADP-ribose) polymerase</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ARTD/PARP family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006637560.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015360879.2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003887"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003895.1">
        <property type="protein sequence ID" value="ENSLOCP00000003887.1"/>
        <property type="gene ID" value="ENSLOCG00000003293.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698771"/>
    <dbReference type="KEGG" id="loc:102698771"/>
    <dbReference type="CTD" id="25976"/>
    <dbReference type="eggNOG" id="ENOG502QQXA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155368"/>
    <dbReference type="HOGENOM" id="CLU_014825_0_1_1"/>
    <dbReference type="InParanoid" id="W5M679"/>
    <dbReference type="OMA" id="IEEANCR"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003293">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018123">
        <property type="entry name" value="WWE-dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF7">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE TIPARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="8">
        <location>
            <begin position="327"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="9">
        <location>
            <begin position="452"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="660" mass="74376" checksum="72B4828E53FA2F4F" modified="2014-04-16" version="1">MEKTLGACSKRTETPDKSPDAPSLGLAKTSGEFSSQMPLSDKIPLVKPYLKKKHAQRKLDAKCLRALDPLFTTLLNSDALVGGDGVFVPRNQPRNQTNLCEAAVEKNSTLSQICLKEDGAVSGLVSGAETDEISDSGAGLGQCEQKAAVGTPEEPRFQAQASEEKNPGVVVSPGQGAPMSGLYAAPEPLRGASCLAEKDADLKSGIFQDKSEEASLDLVFELLTQLQYHTHQGDGVSICVDFLRGVCVYGSDCPQHHTVLPYHWQIRKADTRIWLSVSDDSQEHLERLYCNPDNDQVRLKYQGRVFSLDFSLMQVCDVEFDLVRRLATPSSTSLTSNCHMVWKYYCRDSFGWREYSEPVVRLIEEATCRGLKEVRFVTLQNQYILNIREGFQQNAIFGFRRQIKRRPLFLSSVMLMPYLQTLCGLSSAIFPTAGPTPTQPLSPTSPTSPKVYPETWLPMDSLQDFIQVPVSKDDKSYRTVYSLFHKTVSETKFRILKILRVQNPFLWEKYKRKKEYMSRKMTEVDRVLNERHLFHGTSQDVVEGICKHNFDPRVCGKHATMFGQGSYFARKAVYSHNFSKRSPRGIHCMFLAKVLTGKFTVGNPSMRRPPPLNPHDASSDLFDSCVDNWVDPQIFVIFNDDQSYPYFIIQYEEVSNTVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6P7</accession>
    <name>W5M6P7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Melanoma cell adhesion molecule a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01024165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642304.2">
        <property type="nucleotide sequence ID" value="XM_006642241.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6P7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004055"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004063.1">
        <property type="protein sequence ID" value="ENSLOCP00000004055.1"/>
        <property type="gene ID" value="ENSLOCG00000003395.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691375"/>
    <dbReference type="KEGG" id="loc:102691375"/>
    <dbReference type="eggNOG" id="ENOG502QV1U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155838"/>
    <dbReference type="HOGENOM" id="CLU_028888_3_0_1"/>
    <dbReference type="InParanoid" id="W5M6P7"/>
    <dbReference type="OMA" id="ANEHMTH"/>
    <dbReference type="OrthoDB" id="6431884at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003395">
        <property type="expression patterns" value="Expressed in larva and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005055">
        <property type="term" value="F:laminin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051116">
        <property type="entry name" value="Surface_Rcpt/Adhesion_Mol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11973:SF18">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN MUC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11973">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN MUC18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865634" evidence="10">
        <location>
            <begin position="25"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="535"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="23"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="137"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="249"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="345"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="430"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="621" mass="68186" checksum="3AF9DDBE89D13B71" modified="2014-04-16" version="1">MALLKTTSSVLVLCSVFATLQVSALVMVTMKDQVEVFQGDTAHIPCRATFSKTPSAVHVQWFVRGSSSRERIYFKDRNVSVADNGTGYTGRISIGDDNTLTIADVQLRDARDFICQVGGMSAGKGENRTELKVYVTPEFPVIEGSHSGFSVTSDEQSEIASCEVRNGYPQPNVTWYRNHSPLHPHENQITVVPQVTKESNELFSVKSSLLYKVSKEDKDATFYCEVTYSAPGSLKMVESERINITVHYPTTKVELVSESPQGLIKEGDTVKLRCRGDGNPPPQYTFYSTRSTSTEETELKSQSDLLTLEEVARTDSGLYQCKTLDLDTFEELTANLEILVHYLDPVVLKPSDTVIAVEGDRLIVSCNALSSLKTQVVWKKKGESISAGNTLTLTEATFDTAGEYVCEVSVPELPGLEKSASLMVLVQGIPRLNMHPETEIEETVGKFVNLTCEARAYPQPKIHWVISGTQDWREVFSHAGEQHVVSVVTVRVTSGLQAACNASNEAGFEEHLFAISATPLVTTSTAPSTTEGSGVIIAVIIVCILLLAILGSVLYFLYKKGRIPCGRSGKQDITKEKTTKDDIVVEMKAEKPAEETVLLQGVNGEKKPPSDQCEKYIDLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5M799</accession>
    <name>W5M799_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gamma-crystallin M2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011537">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M799"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004257"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004265.1">
        <property type="protein sequence ID" value="ENSLOCP00000004257.1"/>
        <property type="gene ID" value="ENSLOCG00000003592.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RXJY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162667"/>
    <dbReference type="HOGENOM" id="CLU_063799_0_0_1"/>
    <dbReference type="InParanoid" id="W5M799"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003592">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF129">
        <property type="entry name" value="CRYSTALLIN, GAMMA M6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="51"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="140"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="325"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="370" mass="45154" checksum="ACBE37076C3643F7" modified="2025-10-08" version="2">MLVDSLFYYILLQIIFYEDRNFGGRSYECSSDCADMHSYFSRCNSIRVESGCWMVYERPNYMGYQYFLRRGEYPDYQRWMGFNDCIRSCRMIPHYQGSYRMRIYEREGFGGQMMEFMDDCASVYDRFRYHDIHSCNVMDGYWLFYEQPNYRGRQYFMRPGEYRRFSDWGGMNPRIGSFRRIMEPWKYPCTVFPRSSSTRTGTSGGAPIRCNSIRVESGCWMVYERPNYMGYQYFLRRGEYPDYQRWMGFNDCIRSCRMIPQLIDSFILLHGPSILVLHEYQGSYRMRIYEREGFGGQMMEFMDDCPNVYDRFRYNDIHSCNVMEGYWMFYEQPNYRGRQYFMRPGEYRRFSDWGGMNPRIGSFRRIMDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7A4</accession>
    <name>W5M7A4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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                <person name="Walker B.J."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
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            <name>Zn(2+)</name>
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
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                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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                <person name="Walker B.J."/>
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                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SBSPO"/>
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        <property type="entry name" value="SBSPON_C"/>
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        <property type="entry name" value="Somatomedin_B_dom"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="RPE-SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920:SF2">
        <property type="entry name" value="SOMATOMEDIN-B AND THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF25031">
        <property type="entry name" value="SBSPON_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867439" evidence="4">
        <location>
            <begin position="28"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="5">
        <location>
            <begin position="30"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="29631" checksum="FF482DD147419967" modified="2014-04-16" version="1">VMRRSLAKGVGLLLLTALLGRLHLAEGGCSQKCCQDKDLTCRTSDWRVDRTYGTCFCDEGCQLTRDCCFDYTAECPARPCVVGEWSPWSGCVQQCRPAYRVRRRRVEQEPRNGGQACPPLEERAGCLEYLSSRGQTCAQAQGAAFITSLAYSKGRRKRDLSAELEDPGYCMEFQMGSLSHHCALESRAHARWMQYLREGYTVCVLCQPPAMSDAGHSCQGDGTGIEGNRILQWQAVGNPRCRGTWRKIQKVEHCSCPVVHSFVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M825</accession>
    <name>W5M825_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">DnaJ heat shock protein family (Hsp40) member B4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M825"/>
    <dbReference type="FunCoup" id="W5M825">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004533"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004541.1">
        <property type="protein sequence ID" value="ENSLOCP00000004533.1"/>
        <property type="gene ID" value="ENSLOCG00000003800.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156826"/>
    <dbReference type="HOGENOM" id="CLU_017633_0_0_1"/>
    <dbReference type="InParanoid" id="W5M825"/>
    <dbReference type="OMA" id="MGRDYYK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
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        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:unfolded protein binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C"/>
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        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 4"/>
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        <property type="entry name" value="Dnaj homolog subfamily b member"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C"/>
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        <property type="entry name" value="DnaJ_domain"/>
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        <property type="entry name" value="DnaJ_domain_CS"/>
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        <property type="entry name" value="DnaJ_subfamily_B"/>
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        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF288">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="345" mass="38411" checksum="A80026DC0643CC51" modified="2014-04-16" version="1">RAARKMGKDYYKILGIQRGASDEDVKKAYRKQALKWHPDKNTAANAEEKFKEIAEAYEVLSDPKKREIYDQFGEEGLKGGPGGCDGQGGNFTYTFHGDPHATFAAFFGGSNPFEMFFGRKAAGGRGEEEDMEVDADPFGAFPGFNLNGFPRERHMGLGQARRRQDPAIHHDLRVSLEEVFAGCTKRMKISRRRLNPDGRTLRTEDKILTIEIKRGWKEGTKITFPREGDESPGTIPADIVFVIKDKPHARFRREGSNIVSPVRVSLRQALCGCSVTVPTVDGKSYTLKCAEVIRPGMRKIISGQGLPFPKNPDQRGDLVVEFDVAFPDSLPASAKDVLKRHLPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8J8</accession>
    <name>W5M8J8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tubulin tyrosine ligase-like family, member 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01025358">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025359">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8J8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004706"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004714.1">
        <property type="protein sequence ID" value="ENSLOCP00000004706.1"/>
        <property type="gene ID" value="ENSLOCG00000003942.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156689"/>
    <dbReference type="HOGENOM" id="CLU_010131_6_0_1"/>
    <dbReference type="InParanoid" id="W5M8J8"/>
    <dbReference type="OMA" id="CDIYWHS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003942">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF154">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="777" mass="86968" checksum="C162D99971EF1C72" modified="2014-04-16" version="1">MSDHYVKVRVQLEQMRVAGLETEPEAASLLASSPGSVDLVSGGRRDAARSAVRTRARSKSRGKRSELDRNTAGADNKEDTPEPEYPPGPMDGTAGQLQHSRSPKDAVLQIKGTGKIPQGKAEANGKTEKEGKTQQGKDDGNKLPKKRKMVTVDTSKAKTSLEALKVSIKQLKWKEFPLGRRVACDIYWHGVSFHDNENVFSGLVNKFPGMIEMLRKINLSRAVRTMQELFPEEYNFYPRSWILPEEFQLFAAQVRIAKDKDPSWKPTFIVKPDGGSQGDGIYLIRDPGDVRVMGNAQSKHAVVQEYIQKPLLVDKLKFDIRLYVLVRSLDPLEIYIAKEGLSRFCTEPYQEPNQKNLDHVFMHLTNYSLNVHSGNFVHSDNTNTGSKRTFSSILYRLASKGVDIKKVWSDIISLVIKTVIALVPELKVYYQADIPAGKPGPTCFQILGFDILLMKNLKPILLEVNANPSMRIEHEQEVSPGVFEYVPSPVDEEVKVGVIRDTLRLVDPGQRKLTNHQSHTPGNGSELQEDILDTVSPDKAEQDSGVKVENNLPSLCLKQVFPKYTKQFNYLRTVERIATLFMRFLGVKGTMRLGPTGFRTFIRNCKLSNSNFSMASVDILYIDITRRWNGMGSDQKESGMGLQAFVEAFFYLAQRRFKSPPLHEQVAALLDLCDCHLESLDEKRSLCGRGAAERRTALPSCLPEGVHSAPSAPAPHHFNCPTAANRLLLRSVPDLSCLEDDAQLILLLAGVLSNHLRSAHCHPAQGEKLKKKKRKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8V3</accession>
    <name>W5M8V3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease LACTB2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Beta-lactamase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8V3"/>
    <dbReference type="FunCoup" id="W5M8V3">
        <property type="interactions" value="1028"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004812"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004820.1">
        <property type="protein sequence ID" value="ENSLOCP00000004812.1"/>
        <property type="gene ID" value="ENSLOCG00000004014.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001710"/>
    <dbReference type="HOGENOM" id="CLU_048478_1_2_1"/>
    <dbReference type="InParanoid" id="W5M8V3"/>
    <dbReference type="OMA" id="WQAMDVV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004014">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07722">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000328">
        <property type="entry name" value="Lactamase beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000017">
        <property type="entry name" value="Lactamase beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047921">
        <property type="entry name" value="LACTB2-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041516">
        <property type="entry name" value="LACTB2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF0">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17778">
        <property type="entry name" value="WHD_BLACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="9">
        <location>
            <begin position="31"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="288" mass="32308" checksum="F13654AF85FD6A68" modified="2014-04-16" version="1">TMAAFIPRIEQLSSRVVRILGCNPGPMTLQGTNTYLVGTGKRRILIDTGEPSVPEYISCLKQALTQFNTTIQEITVTHWHIDHSGGIADICKDITKDSDLRITKLPRNPHVEEATLEGKTYMYLKDGDVLETEGATLRVLFTPGHTDDHMALILEEEKAVFSGDCILGEGTAVFEDLYDYMKSLEVLLSSKADLIYPGHGPVVHNAGSKIREYIVHRNLREQQILQVLQENAGKSFSSMDLVKEVYKETPEVLHKAAEVNLVHHLKKLEKEGKINKEHLPAVRWRSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8Z3</accession>
    <name>W5M8Z3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216515.1">
        <property type="nucleotide sequence ID" value="XM_015361029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8Z3"/>
    <dbReference type="FunCoup" id="W5M8Z3">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004852"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004860.1">
        <property type="protein sequence ID" value="ENSLOCP00000004852.1"/>
        <property type="gene ID" value="ENSLOCG00000004062.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685346"/>
    <dbReference type="KEGG" id="loc:102685346"/>
    <dbReference type="CTD" id="550415"/>
    <dbReference type="eggNOG" id="KOG0934">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193372"/>
    <dbReference type="HOGENOM" id="CLU_061221_1_3_1"/>
    <dbReference type="InParanoid" id="W5M8Z3"/>
    <dbReference type="OMA" id="CCAMEEG"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004062">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000005">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="154" mass="18468" checksum="20CE17735DC81EBE" modified="2014-04-16" version="1">MMRFMLLFSRQGKLRLQKWFMAIGEREKKKIIRDMTQMVLARKPKTCNFLTWKDLKIVYKRYASLYFCCAMEEGDNELLALEILHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFLMGGEIQETSKLLISKSIEESDMLQETMEEYMSKPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9L7</accession>
    <name>W5M9L7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycolipid transfer protein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accelerates the intermembrane transfer of various glycolipids. Catalyzes the transfer of various glycosphingolipids between membranes but does not catalyze the transfer of phospholipids. May be involved in the intracellular translocation of glucosylceramides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLTP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005104">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640268.1">
        <property type="nucleotide sequence ID" value="XM_006640205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9L7"/>
    <dbReference type="FunCoup" id="W5M9L7">
        <property type="interactions" value="1025"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005076"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005084.1">
        <property type="protein sequence ID" value="ENSLOCP00000005076.1"/>
        <property type="gene ID" value="ENSLOCG00000004248.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686443"/>
    <dbReference type="KEGG" id="loc:102686443"/>
    <dbReference type="CTD" id="558007"/>
    <dbReference type="eggNOG" id="KOG3221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155182"/>
    <dbReference type="HOGENOM" id="CLU_079400_2_1_1"/>
    <dbReference type="InParanoid" id="W5M9L7"/>
    <dbReference type="OMA" id="EMHGAEW"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004248">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000003">
        <property type="entry name" value="glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF97">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="209" mass="23855" checksum="155A91D6C721C9E2" modified="2014-04-16" version="1">MALLAEHQFKQLPADKQVDTRSFLESVSYLPSFFDCLGSTVFSPIKADVSGNITKIKAVYDTNPTRFKTLQHILEAEKEMHGAEWPKVGATLALMWLKRALKFIQVLLQSIADGERDDTNPNLIRVNATKAYEMALKRYHGWLVQKLFKAAVYAAPYKSDFLKALSKGRDVKEEECLEKIRQFLVNFTATVDAIYEMYSKMNAELDYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA38</accession>
    <name>W5MA38_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ADAM metallopeptidase domain 8b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025211">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA38"/>
    <dbReference type="FunCoup" id="W5MA38">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005247"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005255.1">
        <property type="protein sequence ID" value="ENSLOCP00000005247.1"/>
        <property type="gene ID" value="ENSLOCG00000004374.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158585"/>
    <dbReference type="HOGENOM" id="CLU_012714_7_1_1"/>
    <dbReference type="InParanoid" id="W5MA38"/>
    <dbReference type="OMA" id="HGQDHCL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004374">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051044">
        <property type="term" value="P:positive regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF20">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="14">
        <location>
            <begin position="202"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="13">
        <location>
            <begin position="409"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="633"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="824"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="875" mass="96160" checksum="D3124E5F67CC800F" modified="2014-04-16" version="1">ASQNTIQMRIYIFTSLYATNIALSSLPDVEKYEVISPRRLNNAREKRSLSTDKRLYSEELRYALTIEGRNYTLHLEKNRNLLGKDYTETHYLKNGTEVTSTPNLKDHCYYQGHIQGMNDSSASVEVCSGIRGLVRAGQKLFLIEPLGGSADGEHALYRQEHLRRKRSACREANRTVFDKDPKPAGFIRIQSWRSAPVVKGPKYVELFLVVDNTEYKQHNKDMSQIRRRMMDVTNHIDKLYRTINIRVMLVGLEVWTDWDKIDVSTDPDKTLTRFLEWRKNVLLKKKRHDNAHFVTGISFVGSTVGLANKFAMCTINSAGVNEDHNPNSIGLASTIAHEMGHNLGMSHDTESCYCEKSLSPKGCVMAPSVGDVFPQSFSSCSQQELESFLNQISSACLNNVPSAGDLFGGPECGNMFLEPGEECDCGTVEECRNPCCNATTCRLTEGAQCAQGECCQACKLKPAGVVCRKSAGDCDLDDFCSGLSAECPKDDFKMNGLPCENNQGYCFNGQCPTHLQHCQILWGPGAQVAPDLCFKNNQQGNLHLFCKKTRYGPQPCAQEDVKCGKIHCTGGSDLPVTQSKYVLTMSWPQLLKCNVAEVTDTEAEDIGLVPTGTKCGDNKVCYGNACKDIAVYGTKGCSEKCNNHGVCNHERKCHCDPGWAPPYCDVKLSELPQGGSAVVIAVSVVVTILVLLPLIVGGLICCRRGRKEVYSSKKRAHSASGLSNPLFHGVSTKSSPRCAPPRISTPTFLESSASPLPCAPLRSTVTPSRAPPQIVKPSQPPPLPPPETVCFQVKTHKYLMIKETGVLKIYVLYLFQAKPPAPKKPLPDLSVKQVAKPNSAPPVPPVKPPGSAPHRKQPQVAGKPKTALMPPVKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MB40</accession>
    <name>W5MB40_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA-binding protein Musashi homolog 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with RHOBTB2; the interaction is necessary for MSI2 ubiquitination and degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Musashi family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005745">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222893.1">
        <property type="nucleotide sequence ID" value="XM_015367407.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MB40"/>
    <dbReference type="FunCoup" id="W5MB40">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005599"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005607.1">
        <property type="protein sequence ID" value="ENSLOCP00000005599.1"/>
        <property type="gene ID" value="ENSLOCG00000004675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685567"/>
    <dbReference type="CTD" id="394084"/>
    <dbReference type="eggNOG" id="KOG4205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155420"/>
    <dbReference type="HOGENOM" id="CLU_012062_3_0_1"/>
    <dbReference type="InParanoid" id="W5MB40"/>
    <dbReference type="OMA" id="RAGASPX"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004675">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12760">
        <property type="entry name" value="RRM1_MSI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12323">
        <property type="entry name" value="RRM2_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000020">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034126">
        <property type="entry name" value="MSI_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="110"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="41628" checksum="879472CF291616A1" modified="2014-04-16" version="1">MEGDGSQATSGSPNDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLSYPNIVATYGRGYTGFAPSYSYQFPGFPATAYGPVAAAAVAAARGSGFPDYGFYSSPSDQRGPPCSFADYGSLGPQAAQMLSEHATSASNSPLHQLHSPDQFKSPGTNPPRPGGFPGANSPGPVADLYGTASQDSGVGNYISAASPQPGSGFGHSIAGPLIATAFTNGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBA9</accession>
    <name>W5MBA9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neurocan core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chondroitin sulfate proteoglycan 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011076">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011078">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011079">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011080">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MBA9">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005668"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005676.1">
        <property type="protein sequence ID" value="ENSLOCP00000005668.1"/>
        <property type="gene ID" value="ENSLOCG00000004727.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQ78">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158649"/>
    <dbReference type="HOGENOM" id="CLU_000303_0_1_1"/>
    <dbReference type="InParanoid" id="W5MBA9"/>
    <dbReference type="OMA" id="HESGHWN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004727">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
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    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000571">
        <property type="entry name" value="Neurocan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000537">
        <property type="entry name" value="Notch 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF24">
        <property type="entry name" value="NEUROCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="109"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="211"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1106"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1144"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="22">
        <location>
            <begin position="1193"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="24">
        <location>
            <begin position="1311"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="368"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="488"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="682"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="797"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="842"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1011"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="682"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1132"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1170"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1313"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1342"/>
            <end position="1369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1369" mass="148822" checksum="C6C65085FD3E0D73" modified="2014-04-16" version="1">MVREELAGVAFLPCVFTLRPSPSHEPPRIKWTKMLSGREQSVLVAKDNVIKVKKDFQGRVTLPGYPDNRYNATLALTGLRSSDSGLYRCEVVVGIDDEQDTVPLEVTGVVFHYRAPSDRYALSFRDAQRVCLENSAAIATPSQLQATFDDGYDNCDAGWLSDQTVRYPIRSPRPGCYGDREDQPGVRNYGDRDPAELYDVYCFSRQMKAGEVFHTAVPEKLSLATASTHCHSLGMQLATVGQLYLAWQDGLDRCDPGWLADGSVRYPINHPRRNCGGDEPGVRTVYQNPNRTGFPDTTSLFDAYCYSGTLFPSLPGSESGVWICTPEKVFVLNAPSELSSLASNDSAEMSGEHVVMHVGPSSLRLREPAEQNAEGPPGTRPQAASPGPAPEGEPSLESLEQQGGSAREEEGAEEEVEGGTREPAQSTISPGKGPAAEPTGEAIDGDSKPETSHFTTQNAILEHISVKPSHGASAGDGLTGSNKEISDQERETFFESSNSNAAAERDQADDRGWPAGHAYTVEEHNKAGSGEMGMVLLCGKALLNAFSCSLQTVAHSRAVPARPPCYCRVWELSRLHSRFLFITVQTHKDLTSTVTVAPLNLEGRQSGQSTLHPVEEKETLHDRYVTEAGPHPPVVATTPSTWATSLSFVPIEPLATIRTATAEAYASDVPLATQKQLLQTDGAQGRLSSQTTHGPVVRQSPAQSGLSAPTGGPSEDLDEPTELPAVSVTRSPEKMTQGEVDSNHTEESPPLWSDRPESSSFSEPPAVAGLDFKDSVGEAITHSVFFDYTPVVQAGKPTLDASPVEKSAGPEDRPSMVAATTDGPPSATSQSQVLTSALMESHQSSHTWYNEEEEKNTTTPGPDFSPSTAMSGLTASTGAAKNKRQIGVPLNTGEPLNYLRANKGQTDQKRDRCSSGSGELVCVLSSPPLLPLLPCSSAPSIPRGFGIYLEIHLQVIERHLFIYFFFFPPYSPLCSAFWHRAPALTPACAASQIETLTRLLHQAPMKELFPPRPASATLPGKERSATAGKSRTASPHYVTHMLYSSRWRITASSSPNPIPGVSNLASKSSLVPPHHLASLSPPSRACALAHVSSGTGHVCRVPPAAENDPCQTNPCLHGGTCLSTGDLYSCQCQPGYSGENCEIDIDDCQSTPCQNGGTCIDEINSFVCLCLPSYGGATCEKDTEGCEHNWRKFHGHCYRYFTHRHTWEDAEKDCREHNGHLASIHTPEEQDFVNGLGRENAWIGLNDRTVEEDFQWTDTMAVQYENWRENQPDNFFAGGEDCVVMIAHEHGKWNDVPCNYNLPYICKKGTVLCGAPPTVENAFLIGRKRTHYDIHSVVRYQCADGFLQRHVPTARCRSNGRWDHPKIIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MBX6</accession>
    <name>W5MBX6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01013462">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MBX6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005885"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005893.1">
        <property type="protein sequence ID" value="ENSLOCP00000005885.1"/>
        <property type="gene ID" value="ENSLOCG00000004890.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002762"/>
    <dbReference type="HOGENOM" id="CLU_022477_0_1_1"/>
    <dbReference type="InParanoid" id="W5MBX6"/>
    <dbReference type="OMA" id="ANASVMH"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004890">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000314">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF59">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="12">
        <location>
            <begin position="30"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="489" mass="54294" checksum="630A632265C642BF" modified="2014-04-16" version="1">RADMAHNNGNHGDQSKDTWDSHNRIMREPLSSNDPEVFEIIKNEKKRQIYGLELIASENFTSRAVLEALGSCMNNKYSEGYPGQRYYGGTEFVDELERLCQKRALEVYSLDAEKWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYIDYDRLAENARLFHPKLIIAGVSCYSRNLDYARMRQIADENGAYLLADMAHISGLVAAGVVPSPFEHCDVVSTTTHKTLRGCRAGLIFYRKGVRSVDPKTGKESLYNLESLINQAVFPGLQGGPHNHAIAGVAVALKQALTPEFRAYQVQVVANCRAMAATLTDLGYKIVTADTSSHMRIRFQLSDPGEPGLKRKTEIRKPFVTGILPVSPGDKSALRPSGVRLGTPALTSRGLLEDDFRKVAEFIHRGISLTVEIQRDMNPKATLKEFKDKIAQDEKVQMRIKEIRDGVEAFAGRFPMPGLPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MF11</accession>
    <name>W5MF11_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Myoferlin</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferlin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025156">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000006978.1">
        <property type="protein sequence ID" value="ENSLOCP00000006970.1"/>
        <property type="gene ID" value="ENSLOCG00000005726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154741"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005726">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08373">
        <property type="entry name" value="C2A_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04011">
        <property type="entry name" value="C2B_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04018">
        <property type="entry name" value="C2C_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000009">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000021">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000033">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037726">
        <property type="entry name" value="C2A_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037720">
        <property type="entry name" value="C2B_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037722">
        <property type="entry name" value="C2C_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF55">
        <property type="entry name" value="MYOFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08151">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2027"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="186"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="343"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1121"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1285"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1534"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1768"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="125"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1977"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1977"/>
            <end position="1989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2062" mass="233776" checksum="3EAFA9F539A7F117" modified="2014-04-16" version="1">MLRVAVESAKGLPKKKLGNPDPIVTVVFKAEKKKTKSINSETNPVWNEVLEFDLKATPLDSSSFIEVIVKDYETIGQNKLIGSAKVSLKDLATGQVKSLPLKNLPLLNEKNQDIGATINLTIGYEPPATAPSNPNVQTDGGEVAGGGDTGGDGEEEGGEEHVDGGQGGLSGDPLSPGQPGNQLQKRTGRRKRNRTLPNKPQDFQIRVRIIEGRQLPGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDEIFFYNVNMLPSELFDEDISIRVYNSYSLRADSLMGEFKLDVGYIYDEPAHAVMRKWLLLNDPDDSTSAAKGYLKVSMFIVGTGDEPPVERRERSEEEDDIESNLLLPAGVTLRWVTFSLKVFRAEDIPQMDDAFVQSVKEMFGGDSDKKNLVDPFVEVSFAGKKLCTKIVEKNANPEWNQLINVQAKFPSVCERIKLTVFDWDRLTRNDTVGTTYLQLSKIASSGGEVEGIESHSRYGASPASEVNTGESEVGFLPAFGPCYINLYGSPREYTGLPDPYDELNFGKGEGVAYRGRILVELTTKLDGKAEKKVEDIPSDDILVTQKYQRRRKYSLCAVFHTASMLQEIGEPVQFEVSIGNYGNKFDLTCKPLASTTRYSCAVFDGSFYYYLPWSDSKPVVILNSYWEDISHRLDSVNIILYIAERLQSNITSLKTALQAKMSETRQAEIWLKLVNQLIEDLSTFRLPEMEGKPNLTNLDIQIKKLRDSTIGSILQGAQRMKEEATDVKATLVDIEEWLESLKQVAEEPQNSMPDVIIWMLRGEKRVAYTRIPAHEILYSTHSEHANGKFCGKTQTIFMQYPMDSNKGLKIPVQLRINMWLGLSANEKKFNSFSEGTFSVFAELYENQALVFGKWGTTGLLNRHKFSDVTGKIKLKRESFLPPQGWEWEGDWFVDPEKTMLTEADAGHSDFIDEVFQNETRFPGGEWKQAEVPFTDVNGEKAPGPQEIECPPGWTWEDEWSYDINRAVDEKGWEYGVTIPPDDKPKSWVPAEKMYHVHRRKRMIRPRKKVSMHLGFTDQQKKFLGDPEGWEYSSLIGWKFHSKERTSDTFRRRRWRRRMAPSGRLGASAIFKLEGALGIDEEEKGSKKESATSLFGANTPTVSCNFDRSFVYHLRVYIYQARSLLPMDKDSFSDPYAHVSFLHLSKKTEIIKATLNPTWDQTLIFNDVEIYGDPQAVSQNPPYVVMELYDSDQVGKDEALGRSMCPPMVKLNPGTDVAPKLLWYPIMYKGKRAGEVLVAAELILKNTGTESDLPIVPPKRGEKLYMVPQGIRPVVQLTAIEILAWGLRNMKTYQLAAVTSPSLVVECGGEIVQSPVIKNIKKNPNFPGSLLFLKVLLPKEEMYTPPIILKVIDHRPFGRKPVVGQCTISTLEEFRCDPYTAKPELAMTSKVALMSAPRDVIIDIEDKRPLLEAQHAEKEKETVDWWSKFYSSIGEHEKCGPYLQKGYDSLKVYDDELEDIPEYRGLTDFCSTFKLQRGKTDEDDEDPTVVGEFKGSFRVYPLSDDPGVPAPPRQFRELPDSGPQECLVRIYIIRGIDLQPKDNNGMCDPYIKISLGKKTIEDRDNYRPNTTNPIFGRMFEMTCFIPQDKDLKISIYDYDMLTRDEKVGETVIDLENRFLSRFSAHCGLPQSYCVSGINQWRDQLKPSQILQNLARLKGIPPHVTLDNGTSLTFGGREYNLADFEANKMIHQHLGPPAERLALHVLRTQGLVPEHVETRTLYSSFQPTLSQGKLQMWVDVFPKSLGLPGPPVDVTPRKAKKYVLRVIIWNTTDVTLDETSITGEHMSDIYVKGWMPGMEEGKQKTDVHYRSLDGDGNFNWRFVYDFEYLPAEQLCLVSRKEHFWSLDKTEFRTPPKLIIQIWDNDKFSLDDYLGTVELDLHRLISPAKTPEKCSLNMIPEIGGSTPPKALHACSLFDQKSVRGWWPCMMEENGKKVLGGKVEMTLEILAEKEAEERPAGKGREEPNMNPKLDPPNNSRPETSFFWFTNPCKTMKFIVWRRFKWIFIGIIILLIVLLFLGILLYSLPNYISMKIVKPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFU2</accession>
    <name>W5MFU2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SH3 and cysteine-rich domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <location evidence="11">Sarcolemma</location>
            <location evidence="11">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01018794">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFU2"/>
    <dbReference type="FunCoup" id="W5MFU2">
        <property type="interactions" value="1109"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007251"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007259.1">
        <property type="protein sequence ID" value="ENSLOCP00000007251.1"/>
        <property type="gene ID" value="ENSLOCG00000005998.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QT6I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183092"/>
    <dbReference type="HOGENOM" id="CLU_048120_1_1_1"/>
    <dbReference type="InParanoid" id="W5MFU2"/>
    <dbReference type="OMA" id="NEEWWRI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005998">
        <property type="expression patterns" value="Expressed in muscle tissue and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010880">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033292">
        <property type="term" value="P:T-tubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20882">
        <property type="entry name" value="C1_Stac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000022">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000167">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF2">
        <property type="entry name" value="SH3 AND CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16664">
        <property type="entry name" value="STAC2_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="16">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="43068" checksum="4E9E1370F4403F7B" modified="2014-04-16" version="1">MAQYDQISDKVSLDPHDIPPSPGEDNALWKLKKLFQRKKRSQEADGGEQPNGELANTGPVYFIYEEEEEEDDDQKDEPPPEPVKPINDKPHKFKDHYCKKPKFCDVCARMIVLNNKFGLRCKNCKTNIHHHCQSYVEFQKCFGKIPPGFRRAYSSPLYSNQQNACVKELLPFSQSNRTDPVFETLREGVIMANKERKKGSDDKKNVMMMMMEEEESQAPKPEEGKAEGENPEGDKKGDKAAADDKNKKSQPGMRTGFTQSHYYLAVYRFKALEKDDLDFYPGDRITVIDDSNEEWWRGKIGEKTGYLPANYIIRVRAGERVYKVTRSFVGNREMGQITLKKDQIVVQKGEEVNGYLKVSTGRKLGFFPADLLQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFV1</accession>
    <name>W5MFV1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Metaxin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFV1"/>
    <dbReference type="FunCoup" id="W5MFV1">
        <property type="interactions" value="1428"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007260"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007268.1">
        <property type="protein sequence ID" value="ENSLOCP00000007260.1"/>
        <property type="gene ID" value="ENSLOCG00000006008.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182919"/>
    <dbReference type="HOGENOM" id="CLU_044137_5_1_1"/>
    <dbReference type="InParanoid" id="W5MFV1"/>
    <dbReference type="OMA" id="ANYFFGN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006008">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03212">
        <property type="entry name" value="GST_C_Metaxin1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03078">
        <property type="entry name" value="GST_N_Metaxin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017410">
        <property type="entry name" value="Metaxin1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF30">
        <property type="entry name" value="METAXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="11">
        <location>
            <begin position="174"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="279"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="310" mass="34730" checksum="128DA56FA31F1968" modified="2014-04-16" version="1">MATPMELICWGSDWGLPSVHTDSLIVMAYAKFSGAQLKVLPIDLTWRTLTGTVPALEFDGTTVTEPVHILSYLRKQQRYNADYELTAKQGADTLAFIALLEEKLRPALLHTFWVDVENYSNVTRPWFASRTPFPLNFFLPSRLSSEALSRIMLTKGEPPLHSISEVEAKIYSEAKECLNLLSHRLGTSQYFFGNMPASLDAFVFGYIAPLHKADLPNGQLQVHLKQLTNLGQFCDAIIKTYFVESASGLSPASQDAVDANLQKLTQLVNKESNLIEKMDDNLRSSPQHRPRRSDLKPSLISEERNSSMPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGC3</accession>
    <name>W5MGC3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SET binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01014663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007432"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007440.1">
        <property type="protein sequence ID" value="ENSLOCP00000007432.1"/>
        <property type="gene ID" value="ENSLOCG00000006153.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683162"/>
    <dbReference type="KEGG" id="loc:102683162"/>
    <dbReference type="CTD" id="26040"/>
    <dbReference type="eggNOG" id="KOG1083">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158784"/>
    <dbReference type="InParanoid" id="W5MGC3"/>
    <dbReference type="OMA" id="QEPEQMN"/>
    <dbReference type="OrthoDB" id="9937744at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF2">
        <property type="entry name" value="SET-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
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        </location>
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        <location>
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            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="808"/>
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        </location>
    </feature>
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        <location>
            <begin position="1110"/>
            <end position="1179"/>
        </location>
    </feature>
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            <begin position="1195"/>
            <end position="1225"/>
        </location>
    </feature>
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        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
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        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
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        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
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        </location>
    </feature>
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        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
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        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
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        <location>
            <begin position="851"/>
            <end position="877"/>
        </location>
    </feature>
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        <location>
            <begin position="1149"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1162"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1195"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1212"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1512" mass="168187" checksum="91DEA7FEADD65B7E" modified="2014-04-16" version="1">MEQRELPGPTRPKGGEGVEFRPGGSTPIPGPIPEDSGSPLPLPGKGLPVAGQRAEPEEEEDLGLARDVDANSNPDSEKWAQGDGLEEQEFSIKEASFSEGSLKLKIQTTKRAKKPPKSLENYICPPEIRITIKQPGEQKVAKPGKSSKGTKEEDKGLPKKKFTKNVRGDSSRKIYEKSFKPTEQNEGEHPVDFLGESMKPKHQPRSTQHQLDWSKTPVGGTISQGSLTETESKREPTVVNKSSFPDSANGLAKPQQKKVTGSPSVTHFSTGLSSQVSGSLVQPSSIASMPQMPNQSESSRMILNSLNKDRVVQEAAEPIFGNIKRKYGRKDSMRSLVAQNPEAQWGKKAEKDFDAKIPETIKEKEVHEPEKTAESPCGNSESSGKVLEKEGDIIPGAVPLQIEDSKGYPGKKRRYRRSFKDQLPVENLLEEIQTSDLAEKNDVGNRGLPEPKAAIINKTESRTSKLMEKEGPHEADEDNTVSVTEFSEMCRNRLRKPLPVVRPKMVLDSNQAINKEDNDKLPKPKAKARWSYIKSKNCSLQKDDSSPAAVVAEPPSAYPITPSSPLYTNTDSLTVITPVKKKRGRPKKQPLLTVETIHEGTSTSPVSPIARESPGSLKKRRKKRSLIKLVQIAAANASPANQMKLNKKVGNLGILDKKTIRTINKMKMVKRKNILNEIYSNLALQSKVPRSNVVSTMASTIEARLGKQINVSKRGTIYIGKKRGRKPRAEVQVQQDEHKTSDKHPVPVPSQFENPVVPCNPQPTAGMPSPRATQPSVAHAAGTGGLLCPATADTSLQELKTMPNLQPISALPTKAPKGLHSSNWKLSPPRLMANSPSHLSEVASLKEVTLSPVSESHSEETIPSDSGIGTDNNSTSDQAEKGPASRRRYSFDFCTLEPSEAAALEASKKAKRGHCSKHVTAVAVDSYLAHESLKKQKHRRKRKGLQSRDDLQFLADLEELIGKFQVFRVSHRSYSFYHENSYPSIFRINFDHYYPVPYFPYDPLHYLRRNSEMKSKKRRGRPAKANEPMTKMPFLQGFGYPIPSGNYYAPYAMPYTSMPIATSMMNLGYYGQYPAPLYLSHTLGAATSPFIRPAVPPPQFHSGAHVKLTAAAKHKTKHSSHQMPSTGMDEAQSSLVAPKVGASSLPSGRLHKRKHKHKHKHKEDQLSSSQREDLGGLFSGAKNPSYLSLLSERLGMPDKEPSLPKHKDKQRNQQSTDTLQRSSRNIFEVDTLSTLSLSDSQQWKRTRERTESAGDFHNACTRQHAETIRTSSDLFGELQSSRDGESGMNSRRRSLESFGIYREQNIVPFKNGRKEKVQQMYNCPSPSLTDNSPPLKKRFKRKEIEEIQCEVRKMCTFSKILSTKKNLDHVNKILKAKRLQRQAKTGNNIVKRRRGRPRKQPVLLDDESLGQMPVLEKCIDLPGKRSLRTNVVPETLEFAKQDSIMDAIESVVHMARVQQKSGKRWHQSQEDVRVKRPRRCRGMEKDEVVVPGRKLGSSALSSTSFSTVQSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MH80</accession>
    <name>W5MH80_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018861">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MH80"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007739"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007748.1">
        <property type="protein sequence ID" value="ENSLOCP00000007739.1"/>
        <property type="gene ID" value="ENSLOCG00000006407.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160027"/>
    <dbReference type="HOGENOM" id="CLU_051508_1_0_1"/>
    <dbReference type="InParanoid" id="W5MH80"/>
    <dbReference type="OMA" id="YMENGSE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006407">
        <property type="expression patterns" value="Expressed in zone of skin and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000094">
        <property type="entry name" value="Homeobox protein Hox-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF11">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="163"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="43"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="400" mass="42941" checksum="880E15B512780FDB" modified="2014-04-16" version="1">MQKALYYENAGPFLEYSCQDPTALGFQQLYSTPAEYQSLTYYLPEDNSPCDPGSDPEHSAAPSGCRVPDLPETPLRHPPLTPSPDSSTTSAQRSPCRSASNPPDRSPGADKARIASTSSSSTHLPKQIFPWMGERRQTSKQKHPSSAVSGESASSGETSPPGGASKRARTAYTNVQLVELEKEFHFNRYLCRPRRLEMAALLNLSERQIKIWFQNRRMKYKKDHKGRAGLGSPLGGLSPSRSPPPGSSFSGELGYEAPLSQPYGKAAPGSSIYGLAAYSAPPYECPPPAAQKRYAATSLAQEYEQLCVPGDSGYGNPSSGYSGAGFAEPHAPALLSSHPPSQLFHLPHPSSASMDYSCPASSPARHGLGPCDPNPSYTELSSHCCTAQDIAQEPPTLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI71</accession>
    <name>W5MI71_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Bleomycin hydrolase</fullName>
            <ecNumber evidence="3 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="3 9"/>
    <dbReference type="EMBL" id="AHAT01006022">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006641174.1">
        <property type="nucleotide sequence ID" value="XM_006641111.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI71"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008080"/>
    <dbReference type="MEROPS" id="C01.084"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008090.1">
        <property type="protein sequence ID" value="ENSLOCP00000008080.1"/>
        <property type="gene ID" value="ENSLOCG00000006688.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696089"/>
    <dbReference type="KEGG" id="loc:102696089"/>
    <dbReference type="CTD" id="642"/>
    <dbReference type="eggNOG" id="KOG4128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001735"/>
    <dbReference type="HOGENOM" id="CLU_038600_0_0_1"/>
    <dbReference type="InParanoid" id="W5MI71"/>
    <dbReference type="OMA" id="QSYTFFW"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006688">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000021">
        <property type="entry name" value="Bleomycin hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="453" mass="52356" checksum="E9E8F212C383B15C" modified="2014-04-16" version="1">MDTGLSTEKIASFSKRLKAEPRFLLAQNVSTCSDPLEVCLHRQTVQDTVHVFQHSIPTEGKPVTNQKNSGRCWIFSCLNVMRLPFMKKFNIEEFEFSQSYLFFWDKVERCYYFLKVFVETAQRNEPVDGRLVQFLLSNPTNDGGQWDMLVNLIEKYGVIPKKCFPESHSSEASRRMNDILNHKMREYCLRLRNMVASDSTKEELSKAMDSMIEEVFRVASVCLGSPPESFCWEFRDKEKAFHRFGPLTPLQFYREHVKPLYNIEDKICLVHDPRPQNPHKKLYTVEFLGNMVGGRKTLYNNQPIAVLKKAAADSIKEGEAVWFGCDVGKHFHGKLGINDMNVFNHELVFGISVKNLSKAERLMFGDSLMTHAMILTAVTDKEGKEQQYEKWRVENSWGDDRGNKGYLIMTDEWFSEYVYEVVVDKKFIASDVLEVMKQEPVVLPAWDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIP1</accession>
    <name>W5MIP1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01006055">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIP1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008250"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008260.1">
        <property type="protein sequence ID" value="ENSLOCP00000008250.1"/>
        <property type="gene ID" value="ENSLOCG00000006827.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502ST11">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1517432_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIP1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006827">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866176" evidence="1">
        <location>
            <begin position="22"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="177" mass="18582" checksum="32F36D24EDA84037" modified="2014-04-16" version="1">MITTAVLLCVLICSGLRCVRSESLAEAGLLACELVEERVSSGLSGAPETQFVNRKAALLLARPGREADTAQRLQEAQRAGVVTFLATGSAVDVTRHVPGGTEQLQCEIRRYSTGGIQVPWKGTAGPDSAWFTCTLRHSANEFIVTAFLLYPSPAPQQPIGDADTLNTSGGALAADPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJE5</accession>
    <name>W5MJE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004090">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJE5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008504"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008514.1">
        <property type="protein sequence ID" value="ENSLOCP00000008504.1"/>
        <property type="gene ID" value="ENSLOCG00000007011.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005739"/>
    <dbReference type="HOGENOM" id="CLU_009437_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJE5"/>
    <dbReference type="OMA" id="FPFHSEQ"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007011">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.30:FF:000001">
        <property type="entry name" value="Exocyst complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000001">
        <property type="entry name" value="Exocyst complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF2">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="49"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="413"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="818" mass="94556" checksum="F6DB6419A1A46D51" modified="2014-04-16" version="1">MAETATLESITEHERILQEIESTDTACVGPTLRSVYDDQPNAHKRFMEKLDARIRNHDREIEKMCNFHHQGFVDAITELLKVRADAEKLMGQVTDTNRRLQDAGKEVTVQTEEVIRCRVQQRNIATTVEKLQLCIPVLEMYSKLKEQLDSKRYYAALKTMEHLENIYIPRVSQYRFCQIMAETLPKLREEIKEISMSDLKDFLESIRKHSDKIGETAMKQAQQHRTFNSAVQKQASAGYGKPLYMLNGKGSSQKNGIPSEEGAEEEEEDTDEEVLTAQDLVDFSPVYRCLHIYTVLGDRETFENYYRKQRRKQARLVLQPQSNMHETVEGYRKYFNQIVGFFVVEDHILHATQGLVTRAYTDELWNMALSKIIAVLRTHSSYCNDPDLVLELKNLIVIFADTLQGYGFPVSRLFDLLFEIRDQYNETLLKKWAAVFREIFEQDNYSPIPVENEEEYRTVTSKFPFHDAEIEKQQFPKKLPMSQSVPQIYIQVKEFIYASLKFSESLHRSSTEIDDMLRKSTNLLLTRTLSSCLQNLIKKPHIGLTELVQIIINTTHLEQACKYLEDFITNITNVSPETIHTTRLYGLSTFKDARHAAEGEIYTKLNQKIDEFIQLADYEWTMSESDGRASGYLMDLINFLRSTFQVFTHLPNNNNDHAAMSGKVAQTACMSACKHLSTSLMQMLLDSELKQISMGAIQQFNLDVMQCELFASSEPVPGFQGDTLQLAFIDLRQLLDLFMVWDWSTYLADYGQPTSKYLRVNPSTALALLEKVYRGMKDTSKKNNIFSQFRKNDRDKQKLIETVVKQLRSLVNGMSQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK07</accession>
    <name>W5MK07_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01034806">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK07"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008716"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008726.1">
        <property type="protein sequence ID" value="ENSLOCP00000008716.1"/>
        <property type="gene ID" value="ENSLOCG00000007201.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4222">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000054197"/>
    <dbReference type="HOGENOM" id="CLU_666577_0_0_1"/>
    <dbReference type="InParanoid" id="W5MK07"/>
    <dbReference type="OMA" id="NADSPEW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007201">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002362">
        <property type="entry name" value="CD4-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422:SF6">
        <property type="entry name" value="HEMICENTIN-1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866185" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="194"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="413" mass="46356" checksum="D64FAA15F9690A44" modified="2014-04-16" version="1">LKCCVHVCFTLSSSLCAPCTGENSRSLDLPCSSSHLKSTSWKYSSNGNDYRLIFTIDKSGSQRKGKGHQFPAQGRAKPGGRGLQISPVKKNDSGWYMCEQDQKQLVTHRLLIIEVFTKPEGPFLQNTNVTMHCQVGGRTSEKLKWLSPNGAEEGEGTVEISKISPAHTGNWKCEITDVKKYVETLNIDVLGMNPQPLSKQTAKQGDNVFLPCLLSSPLSYTFRSLNFIRGGWMKDDKPLLDLYVQIGTHMSWNATMFKEIEYPKVNDLKTNLSISLIKVKPQQSGIYNCYLRFKEGTITSTIVLKVEGAPGGTGDTSHSMFGLNMNLWVLVSIAIGSVVLIILIIVAIILLLRKKRLKKRMRKRRSQRQPLTARDYCQCNRYCVASTSSRRFYSVCMHYSAFTLLCCCKFLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKB2</accession>
    <name>W5MKB2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sal-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020888">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MKB2">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008821"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008832.1">
        <property type="protein sequence ID" value="ENSLOCP00000008821.1"/>
        <property type="gene ID" value="ENSLOCG00000007283.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1074">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155938"/>
    <dbReference type="HOGENOM" id="CLU_005740_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKB2"/>
    <dbReference type="OMA" id="QELHKSP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007283">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000708">
        <property type="entry name" value="Sal-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000689">
        <property type="entry name" value="Spalt like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000302">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000341">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000079">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF51">
        <property type="entry name" value="SAL-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="812"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1051"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1079"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1179"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1207"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="630"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="836"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="933"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1154"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="866"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="953"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1363" mass="146371" checksum="A7596517FC86C91B" modified="2014-04-16" version="1">MSRRKQAKPQHFQSDPHLPLPEHNGKICTTFALCTASIICSLQTVQRIYIFCLNSMAASHLNVVRCAKELERLSARTAVPPLGRNESLNVSVEQSWELNSFHVSGDMEISSEHPPSKDNDAHVCSRCCAEFFELSDLLQHKKNCTKNQLVLIVNENPASPSETFSPSSPSDNPDEQMNDTVNNADQVDCSDLSEHNALDKEESMNVDASGTGPAVGTSAVSTSLPHLGNLTELGKFSVINSNVIIENLQSTKVAVAQFSQEARSNGSSKVAIPALMEQLLALQQQQIHQLQLIEQIRHQILLLASQNGEMPVPSSSSPGTLGTSGNPLTTLSSHLSQQLAAAAGLAQSLASQSASISSLKQLTTAAQLPQSNPGSSMVPPGSASSQSIGALVASAVNAQTSDKLPAHAGTLHPQLSNPSIAKSSTPAFGIGSLLSPAANPLLPQPPPGNPMFPSPLPSVGTTVEDLNSLAALAQQRKGKPPNVTSFEPKSSSEEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPYKCNICGNRFSTRGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSMPPEKPITSWLDSKPVLSTLTTSVGLLLPPTIPSLPPFIKKEENHPISITSPASSVKSDSGAVEPSGKSNDGVSEEGEGGTLPTSDGKTEDRNQHSNSLTNVSISSAQDCTADLGFSNSPPMATNPLMPLMSEQFKAKFPFGGLLDPTQASETSKLQQLVENIDKKVTDPNECVICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPLPDNYPESMESDTGSFDERNFDDLENFSDENMDSMENMEDCPDSSIPDTPKSADASQDSFCSSPMPPELSAIASLESQVKMTNQGLVEELPASSVKSVENGSVEGDHMTNDSSSLGGDIESQSAGSPAISESTSSMQAPSPTNSAPAYHKSPSFEEKQQRALQMDNIHAGLTQSPSSSGALDLTSCNPSKDPLSMLFPYRDRGTYKNTACDICGKTFACQSALDIHYRSHTKERPFICTACNRGFSTKGNLKQHMLTHQMRDLPCQLFEPSAPSIASSQSPSILAPSSLSSLIKTEVNGFLSSQDCKEPAGNLVSSSATTSPVLTPAPPRRTPKQHFCNTCGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSAPARRGRRLSVDGPMAFLGTNPVKFPEIFQKDMAARAGTGDPASFWNQYAAAFSNGLAMKTNEISVIQNGGIPTLAGGSPIGGLTGSLEKLHGAEPNAALAGLEKMANTENGTHFRFTRFIEDNKEIVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKD6</accession>
    <name>W5MKD6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Junctional cadherin 5 associated a</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01027000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MKD6">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000008856.1">
        <property type="protein sequence ID" value="ENSLOCP00000008845.1"/>
        <property type="gene ID" value="ENSLOCG00000007305.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QURJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166891"/>
    <dbReference type="InParanoid" id="W5MKD6"/>
    <dbReference type="OMA" id="VMYRMRR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007305">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903589">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028221">
        <property type="entry name" value="JCAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757:SF1">
        <property type="entry name" value="JUNCTIONAL CADHERIN 5-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757">
        <property type="entry name" value="JUNCTIONAL PROTEIN ASSOCIATED WITH CORONARY ARTERY DISEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15351">
        <property type="entry name" value="JCAD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1314" mass="147219" checksum="D08A6A34858FCFBD" modified="2014-04-16" version="1">MYSVEDLLISHGYKLPKTASSSNENRYADCHHEIVENRSGHGTANGYETDTGAYFYSRQAPAKGYFSDNECRESNQRRKAGSGNQGDTQPLGDFLTSNTGPCDGPRGMYSAQPRTQRDVAYWRRRGQDFSVLLDYTDRGEKRGSSFAKPEGTRRAHELPAKEGRNDREWWEESAVLKERSKVQEKWRMAGDRKWQSLGTEEWRPAVGIGRQLSDGDNDKWAQDQRGMKAELGAGHPKAKGKSQSLPRVLSPESLQYVDMPLSSQDVFSSQRMNGKCKYHEEVAGREPWTEKCRAGGHPALLPKPRFSRPLKPPSYEAHQQTRGSSEMIAGDQESRSKDKVSYFCKSAESRQDFLPYEPPGSNTEPPVYIPPPSYKKPQQQKGSQKNINEVSDYKFKREVQQVPERKDAGKCFSRQTGNSWIEYQKDRNVPGRKQMYPGYIDDHLGCIQYIPFDDPRVKHIQGDLCGNSLTDSDKIKNISKRIPKVLEQSSHDSAFSPPEGLFFDMDTSKRSFHEHDSGSNSGLHKESGNSMDQSCVKYEQERSNAIYQNRTARQMPNMDQGFSESVTKVKKFEPGTEADNKRNTKKRLNETIFCLVSVPVHLQPNGDRSDQNNNEKDLSHVESSIDKSIGNLQNQSLLSTSSTDLELQALTGGMMNDKSLKKPQQRKDTDDTKSQQPNRHKELKYSGSWPGDQYRDQETQTSFPEASKGAPQGSPNNQAQIHSSPASESTIEVNLSHGYGYPMKGQMHLNPSSNSAFSRTTSFSNQVKSTALQVPPSGNLDEKTYIAAKKSESKPVASNGKEAFGQFLLKPVSRRPWDAIEELETFNKELQEQIGKRASVDKSIDELDVAYRDILDLNTASANTEHTKSQVCDHKNGTSILEAPRCKLSSSWKNMLESRSAATETDFGDVSAFSRLSKKTVSFSKPSKEEVSMLPQNSPSDCRVVNQGNLLSKEFLHRDVSIPSCISSAEAPRFARQMKQDASTHTNPPSYEDTSVKITGLKKQEGVPLKTEPDRRIKKEHLVPKRSEDKSSSISSQSDRFQGDEGLHCKVTRKSWEGRDMNGGSFVIDDDAESAGWMKQFSLAETQLPTNKKAESEQSEDLSNLFEVKKAEGIPENESIEQRAARILGIDVPTECLCVAEQKGAKQGTEDQVTTGNRSLMKAMFSEEGLERSAGGKILCLKGKHEELSPHSRVLKRQQQENGLANSRRENSSCCTLEKNSVENNLKIFLRSHDMSSEKPLVTNAEENMVQASSCDKKGRVASRMIEALQDKLASSPSRTVMERIIRMKEVDSVSRMRRLSIKSSDSGDEAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKS4</accession>
    <name>W5MKS4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009923">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKS4"/>
    <dbReference type="FunCoup" id="W5MKS4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008983"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008994.1">
        <property type="protein sequence ID" value="ENSLOCP00000008983.1"/>
        <property type="gene ID" value="ENSLOCG00000007401.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SAN0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183126"/>
    <dbReference type="HOGENOM" id="CLU_052667_2_0_1"/>
    <dbReference type="InParanoid" id="W5MKS4"/>
    <dbReference type="OMA" id="RTHNARC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007401">
        <property type="expression patterns" value="Expressed in intestine and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035631">
        <property type="term" value="C:CD40 receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002768">
        <property type="term" value="P:immune response-regulating cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008063">
        <property type="entry name" value="Fas_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052135">
        <property type="entry name" value="TNFRSF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46875">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46875:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01680">
        <property type="entry name" value="TNFACTORR6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045938936" description="TNFR-Cys domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="211" mass="23537" checksum="906DD41D54EC13DE" modified="2025-10-08" version="2">MKLSAAACLLLLAVRLPLALELRCSLERQYTHKGRCCDMCPPGHYLKKECSKSEGTVCNPCEQGKYSYTWDINKECLSCRVCSQGKFEVVVRQCGSTTDAVCGCREGYSCTSDTCEQCVWTKRCGRGQELRTHGSFEFRYECENCTRGTYSDTEGGPCKPWTECHSIGLKPVSPGNHTHNVNKRPFLLTVLVIVSALFGLIFTVIFTNVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKZ2</accession>
    <name>W5MKZ2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sperm microtubule inner protein 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01032456">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKZ2"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009062.1">
        <property type="protein sequence ID" value="ENSLOCP00000009051.1"/>
        <property type="gene ID" value="ENSLOCG00000007461.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S7R5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010327"/>
    <dbReference type="HOGENOM" id="CLU_129931_0_0_1"/>
    <dbReference type="InParanoid" id="W5MKZ2"/>
    <dbReference type="OMA" id="DHCILND"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:axonemal A tubule inner sheath"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR038775">
        <property type="entry name" value="SPMIP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35263">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35263:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22593">
        <property type="entry name" value="SPMIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="142" mass="16370" checksum="313377DF73F42728" modified="2014-04-16" version="1">MTFFGITFLGYQDPFGSRRLSQLQKTQYDQEGDGVGGRQRGTTLPPVNPAPHQALSCELHRGSQERYQELVRRAQTPRSPNQIYRAPLTDSQRYGWWLSGSGQPGSEGSEPWTRVRRFPQRHSEMTKFVKSMSMTHREFSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MLM6</accession>
    <name>W5MLM6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oxidative stress-responsive serine-rich protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Oxidative stress-responsive protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Peroxide-inducible transcript 1 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01024191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MLM6"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009296.1">
        <property type="protein sequence ID" value="ENSLOCP00000009285.1"/>
        <property type="gene ID" value="ENSLOCG00000007656.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUK0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018547"/>
    <dbReference type="HOGENOM" id="CLU_050222_0_0_1"/>
    <dbReference type="InParanoid" id="W5MLM6"/>
    <dbReference type="OMA" id="MSECERP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007656">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008494">
        <property type="entry name" value="DUF776"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31383">
        <property type="entry name" value="OXIDATIVE STRESS-RESPONSE SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31383:SF2">
        <property type="entry name" value="OXIDATIVE STRESS-RESPONSIVE SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05604">
        <property type="entry name" value="DUF776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="177"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="47560" checksum="2F7C5478D6BB72D7" modified="2014-04-16" version="1">MTGARTFYDVSVRSTSEECVLLHSHCEGSECKQDALGGGELTRPLCCSYRECKDSQDACPQHGVRLLRPGAAGSGVRDALSGEAAADSAVQWAWLLQRAPGKSLDVQAQLGQSGGPEPVVDLTSPLRCSSVVFSTMEPGAKDPEEETLQTAFKKLRVDAEGSTAAVRVCDSTAQRMAARGNPDGAKPKTACPKENWHGCMRKTSRGAARSQRRRRSKSPILHPPKFTYCSSKMSPPPGHLKHKSQPEPDGDSTSLGIPAKKELLSPGRCSPVFGATTYEPYGHESSAGAAALEINPKPVADDEAPSVSKSPGCALCPERTSSGSGPPSDFQSLSKLHEAGGCPCLERQCQCHSWQGVEVYSFTGLRDVISECERNMAGTEDTSRVSPRHRTQGGAAASGSPRSCSEQARAYVDDITIEDLSGYMEYYLYFPKKMSHMAEMMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNA9</accession>
    <name>W5MNA9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="2 7">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2 7"/>
    <dbReference type="EMBL" id="AHAT01027200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNA9"/>
    <dbReference type="FunCoup" id="W5MNA9">
        <property type="interactions" value="1910"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009868"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009880.1">
        <property type="protein sequence ID" value="ENSLOCP00000009868.1"/>
        <property type="gene ID" value="ENSLOCG00000008123.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153662"/>
    <dbReference type="HOGENOM" id="CLU_023894_1_1_1"/>
    <dbReference type="InParanoid" id="W5MNA9"/>
    <dbReference type="OMA" id="SLDQHWA"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008123">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043518">
        <property type="term" value="P:negative regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000043">
        <property type="entry name" value="pre-mRNA-processing factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24814">
        <property type="entry name" value="WD40_Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Prp19 coiled-coil region" evidence="8">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="314"/>
            <end position="348"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="349"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="432"/>
            <end position="465"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="465" mass="50089" checksum="43C7F711FA76FE88" modified="2014-04-16" version="1">QLLSEEQLVDIKVSHPIRPKAPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLVVPQAVPASQPTVAGGGEPMEVSEQVGMTPEIIQKLQDKATVLTTERKKQGSQLTAGIREESLCSAAVVAHEDLPSLLLLQGLHSASIPGILALDLCPTDTNKVLTGGADKNVVVFDKNAEHIMATLKGHTKKVTSVIYHPSQVCLSLESSVNVLSHTGGQLCDPLVGTGSVGLWGLVVGVLLTVPLSSLLPQYWAFSDIQTGRVLTKVTDEAAGCALTCAQFHPDGLIFGTGTADSQIKIWDLKERTNVANFPGHSGPVTAIAFSENGYYLATGAQDCSVKLWDLRKLKNFKTITLDHSYEVKSLIFDQSGTYLAVGGSDIRVYICKQWSEVLNFTEHTGLVTGVAFGQHAQFLTSSGMDRSLKFYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MP55</accession>
    <name>W5MP55_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="AHAT01003405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010164"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010176.1">
        <property type="protein sequence ID" value="ENSLOCP00000010164.1"/>
        <property type="gene ID" value="ENSLOCG00000008355.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695169"/>
    <dbReference type="KEGG" id="loc:102695169"/>
    <dbReference type="CTD" id="57194"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157895"/>
    <dbReference type="HOGENOM" id="CLU_000846_3_4_1"/>
    <dbReference type="InParanoid" id="W5MP55"/>
    <dbReference type="OMA" id="QALRCGR"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008355">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000022">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000109">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000023">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF81">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE VA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1079"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1161"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1190"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1218"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1263"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="1047"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="460"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1347"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1463"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1373"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1396"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="690"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="732"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="756"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="799"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="879"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="880"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="881"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="995"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1001"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1021"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1024"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1025"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1025"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1523" mass="172796" checksum="8E88CBB59AA74B5A" modified="2014-04-16" version="1">MARQCGETEGEKGSKLKKKKKNKKDNKTRTVYSNLLYSSAKGNENPNRHYANNKIKTTKYTLLSFLPKNLFEQFHRFANVYFVFIALLNFVPVVNAFQPELALAPVVFILSVTAIKDLWEDYRRHRSDKEINHLDCLVYCRNERKYVEKYWKEIQVGDFIRLRCNEIIPADVLLLSSSDPDRLCHIETATLDGETNLKQRQVVRSFIDLDCEFDPMKYNSIIECEKPNNDLNRFRGYIIHRSGQRHGLYKDNLLLRGCTIRNTEEAVGIVIYAGHETKAMLNNNGPRYKRSKLERQMNIDVFWCVIILVVMCLFSAIGHGLWVFQYGDKRPVFDVLSPDGTDLSPVLSAIYLFLTMIIVFQVLIPISLFVSIEIVKICQVYFIHQDLDLYDEETDSHLQCRALNITEDLGQMQYIFSDKTGTLTENKMVFRRCTVVGDEYSHDANARRLAMYQEVDSEEEECTSKAGTLPRRDSVTSHQSAKVVHRSQSTKSHRRTGSRAEAKRASILSKHTAFSSPMEKDITPDPQLLDKVNECASQMDFMRIHGQSLTQLTPELSDIIDFFIALTICNTVVVSSPNQPRQKVRMRFEMKSPVKTIEDFIKRFTPSRLTSGSNSSSSSSLAANRSTNRTTSSVFSSPSAESTLIKLDEEKPHSPRGLENVLSVVQKGLEKKWGPLEEGELRYEAESPDEAALVYAARAYKCSLVGRLPDQVTVELPHLGMLNFELLHTLGFDSTRKRMSVVVRHPLTDQIVVYTKGADSVIMDLVKPCSTDDAKGKWRRKILNKTQHYLNLYAADGLRTLCIAKKVLSKEEYAGWLKSHLEAETAIQGREELLFQSALRLETNLHLLGATGIEDRLQEGVPETIASLRKAGLQIWVLTGDKQETAINIAYSCRLLDPEEEIITLNAETQEACAVLLEESLHYIQSKFFGNIPDSAAPAFDEDFPAFEMPPSSSFLVHRLGLVIDGKTLAYALDKSLEDKFLAVARSCRSVLCCRSTPLQKSMVVKLVRNKLKVMTLAIGDGANDVSMIQVADVGVGISGQEGMQAVMASDFALPRFQYLQKLLLVHGHWCYSRLANMILYFFYKNAMFVALIFWYQFYCGFSGSAMIDQWYLIFFNLMFSAFPQLITGTLDKDVSSETLLALPQLYKNGQSSEEYKPYMFWMNMIDAFYQSLVCFFIPYFAYADSDVDLFTWGTPITTLSLFTILLHLGIETKTWTWLNWASIIFSITLFFMVAFCYNASCPTCYSPSNPYWTIQRLMADPLFYLFCIVTPVVALLPRYFYRAFQGTLFPSPVQIGRQLDKLSPDTRSNILSQMKIKVGSRFEPRTTFYPLSGPFPKDYSQRTEESHFCKQWNSPKGSGGTGHSTVAAQSVQDKEKGSYRHSTDVAPKVDGPTPESFTASETDTLPYSRESTQHPGDIESPFFEDSLQLDTIMDPSDLSLVKWIATTPLFAKTEGIFESLSSQGSLQATEEKENSSPSNTQYRVDSRDENWQTQQLLKAMSGELKPVASDSLNEDSFQTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS26</accession>
    <name>W5MS26_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01039651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS26"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011185"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011201.1">
        <property type="protein sequence ID" value="ENSLOCP00000011185.1"/>
        <property type="gene ID" value="ENSLOCG00000009171.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162916"/>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_1"/>
    <dbReference type="InParanoid" id="W5MS26"/>
    <dbReference type="OMA" id="CDIRGVF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009171">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="9"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="324" mass="34613" checksum="FB5E0936C6D904E2" modified="2014-04-16" version="1">SYRAALCTELKKPLVLQDVAAAELKPAEVRVAVHFCGVNFADILVCQGLYQEKPPLPFIPGMEFSGCVVETGQNVVEFQKGDRVIGVGGSGMAEQCVVDQKMLWKIPNELSYEEATALPVSYGTGLLALKHRAATQVGETVLVTAAAGAAGLAAVDIASQALQAKVIAAAGSDEKCAIAVQKGAFASINYTTCNLKEELKKLTNKEGVNVVFDTVGGDIFKDALSSLRWEGRIVMVGFAGGAIPSIPANLLLLKNISAMGVFWGRYREENFPLFSQSISQAIHLFQAGKIKPHIGATFKLPQVNQAFHHVLQRQSVGKVVVSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS43</accession>
    <name>W5MS43_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEP domain containing MTOR-interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004829">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS43"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011202"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011218.1">
        <property type="protein sequence ID" value="ENSLOCP00000011202.1"/>
        <property type="gene ID" value="ENSLOCG00000009183.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QS4Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055667"/>
    <dbReference type="HOGENOM" id="CLU_042535_1_0_1"/>
    <dbReference type="InParanoid" id="W5MS43"/>
    <dbReference type="OMA" id="HMAEILV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009183">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
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    </dbReference>
    <dbReference type="GO" id="GO:1903940">
        <property type="term" value="P:negative regulation of TORC2 signaling"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23067">
        <property type="entry name" value="PDZ_DEPTOR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP domain-containing mTOR-interacting protein isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEP domain-containing mTOR-interacting protein isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF18">
        <property type="entry name" value="DEP DOMAIN-CONTAINING MTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="2">
        <location>
            <begin position="48"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="2">
        <location>
            <begin position="148"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="334"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="413" mass="47033" checksum="28FCDAF6FF8F49FC" modified="2014-04-16" version="1">MGLSAANRTHRNRSLEMEGIEHAMQKKAAELERVAEVLVTGEQLRLRLHEGKVIKDRRHHLRTYPNCFVAKELIDWLMEHKEASDREMAIKILQKLLDQSIIHHVCDEHKEFKDAKLFYRFRKDDGTFPLDNEAKIFMRGQRLYEKLMTTDSSLVQTREEDGVKFERTLVASEFVDWLVQEGEASTREEAEQLGRRLLEHGIIQHVTNKHHFVDRGLLYQFKMNFRRRRRLMELLSESCRSIPESHDSPFCLRKQNQDTGNNSFLSVSPTKEIKIVSAVRRSSMSSSCGSSGYYSSSPTLSSSPPVLCNPKSVLKRQVRPEELQTPGGPFIKKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLSLDYRTVSNLILTGPRTVVMEVMEEVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MS76</accession>
    <name>W5MS76_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027390">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027391">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027392">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027393">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631751.1">
        <property type="nucleotide sequence ID" value="XM_006631688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MS76"/>
    <dbReference type="FunCoup" id="W5MS76">
        <property type="interactions" value="1295"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011235"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011251.1">
        <property type="protein sequence ID" value="ENSLOCP00000011235.1"/>
        <property type="gene ID" value="ENSLOCG00000009212.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690068"/>
    <dbReference type="eggNOG" id="KOG1354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
    <dbReference type="InParanoid" id="W5MS76"/>
    <dbReference type="OMA" id="KWCRRTN"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009212">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="51589" checksum="AF53CF55B35A4FD4" modified="2014-04-16" version="1">MEEDIDTRKINNSFLRDHNYATEADIISTVEFNHTGELLATGDKGGRVVVFQREQESKNQPHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKISERDKRPEGYNLKDEDGRIRDPSTITMLRVPVLQPMDLMVEATPRRVFSNAHTYHINSISVNSDYETYMSADDLRINLWNLEITNRSFNIVDIKPANMEELTEVITAAEFHPNQCNTFVYSSSKGTVRLCDMRASALCDKHSKFFEEPEDPNNRSFFSEIISSISDVKFSHSGRYLMTRDYLTAKVWDLNMESKPLETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAVLKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPAENIIAVAATNNLYIFQDKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSI0</accession>
    <name>W5MSI0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Legumain prodomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01000011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSI0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011339"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011355.1">
        <property type="protein sequence ID" value="ENSLOCP00000011339.1"/>
        <property type="gene ID" value="ENSLOCG00000009295.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000256982"/>
    <dbReference type="HOGENOM" id="CLU_1895490_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSI0"/>
    <dbReference type="OMA" id="PCARSIV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009295">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd21115">
        <property type="entry name" value="legumain_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.130:FF:000001">
        <property type="entry name" value="Vacuolar-processing enzyme beta-isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048501">
        <property type="entry name" value="Legum_prodom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046427">
        <property type="entry name" value="Legumain_prodom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20985">
        <property type="entry name" value="Legum_prodom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Legumain prodomain" evidence="1">
        <location>
            <begin position="38"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="134" mass="15424" checksum="15D5ACF204C5B8D0" modified="2014-04-16" version="1">MTSPAVDPVVKSSDVPVVTLQNKIQSAKTPEEKEKLKKDLDDLMQTRELIRASVIQIIEKSTDSKEQAQSIQTSGQADITNYKVYREVIEYYKQKCFNWHNPKYAYALHQLHLFSNLCEANVPMERSCFTVHCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSU6</accession>
    <name>W5MSU6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Squalene monooxygenase</fullName>
            <ecNumber evidence="4 11">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="4 11"/>
    <dbReference type="EMBL" id="AHAT01004789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSU6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011455"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011472.1">
        <property type="protein sequence ID" value="ENSLOCP00000011455.1"/>
        <property type="gene ID" value="ENSLOCG00000009390.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011759"/>
    <dbReference type="HOGENOM" id="CLU_026390_0_0_1"/>
    <dbReference type="InParanoid" id="W5MSU6"/>
    <dbReference type="OMA" id="AKRTFYW"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009390">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000706">
        <property type="entry name" value="Squalene epoxidase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="14">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="15">
        <location>
            <begin position="295"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="577" mass="64551" checksum="2198094D772BCFDE" modified="2014-04-16" version="1">MWTFLGIASFTYVYKKCDAVLHYANKEVALVFLGCCLLASLLSYARYRSGQALPSLGLDIPASLPLARFFSGRDTDSGSRAEKQRARPGEGRRRRRHVGPADSTWPDSECGSAADSRDPDVVVVGAGVLGSALAAVLARDGRRVTVIERDLREPDRIVGELLQPGGYRALRELGLDGKRSHCNCLSFHLVRWYAVRCFNTCSSFVSSFKVNQLSSVSNEKLTRYCKFILLINRIATLSDDVKFIEGTVTNLLEEEGSVAGIQYKDKETGEIKVRHQNLLFLKCPRSNPFAPAQDSLLSNNALLLSEWLRFLFQNSPQFKANHAELVLANPSPVLIYQISSSETRVLVDIRGEMPKNLREYMTDKIYPQLPEHIKEPFLHALQSDRLRTMPASFLPPSPVNKPGDILNLIWFLSISKALTNYGNTICILTFCVNIPAIQKYPSLFYCSAACLILITSRFKKSRKCALSFSLQILPSTAYCVFLISDSLHQLRKACFQYFRLGGECISGPIGLLSVLSPKPLTLIGHFFAVALYAIYFSFKSESWVTMPRALLSSGAILYRACIIMFSLIYSELKYLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSY4</accession>
    <name>W5MSY4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING finger protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">RNF11</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (when phosphorylated) with 14-3-3. Interacts with the E3 ubiquitin-ligases NEDD4, ITCH, SMURF2 and WWP1. Also interacts with the E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2N, but neither with CDC34, nor with UBE2L3. Interacts with ZNF350, EPS15 and STAMBP. After TNF stimulation, interacts with TAX1BP1, TNFAIP3 and RIPK1; these interactions are transient and they are lost after 1 hour of stimulation with TNF. Interacts with GGA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSY4"/>
    <dbReference type="FunCoup" id="W5MSY4">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011493"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011510.1">
        <property type="protein sequence ID" value="ENSLOCP00000011493.1"/>
        <property type="gene ID" value="ENSLOCG00000009419.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110988"/>
    <dbReference type="InParanoid" id="W5MSY4"/>
    <dbReference type="OMA" id="HLDCIDN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009419">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16468">
        <property type="entry name" value="RING-H2_RNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000134">
        <property type="entry name" value="Ring finger protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042981">
        <property type="entry name" value="RNF11_RING-H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052804">
        <property type="entry name" value="UEC_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="102"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="157" mass="17799" checksum="1135B1CD15F737E9" modified="2014-04-16" version="1">MGNCLKSPTSDDISLLHESQSDRASYGDGTDPDQEPPPPYQEQEEQAQVPVYHPTPSQTRLATQLTEEEQVRIAQRIGLIQHLPKGVYDAGRDGSEKKIRECVICMMDFVYGDPIRFLPCMHIYHMDCIDDWLMRSFTCPSCMEPVDAALLSSYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MU17</accession>
    <name>W5MU17_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NOVA alternative splicing regulator 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with PTBP2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MU17"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011876"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011896.1">
        <property type="protein sequence ID" value="ENSLOCP00000011876.1"/>
        <property type="gene ID" value="ENSLOCG00000009727.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155573"/>
    <dbReference type="InParanoid" id="W5MU17"/>
    <dbReference type="OMA" id="KYANTPF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009727">
        <property type="expression patterns" value="Expressed in brain and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000019">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000020">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="48"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="170"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="390"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="49148" checksum="D4FFC2A8245ED635" modified="2014-04-16" version="1">MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAVSTKRTNTGEDGQFFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRASQVKIIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKTTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV67</accession>
    <name>W5MV67_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01021360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631905.1">
        <property type="nucleotide sequence ID" value="XM_006631842.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV67"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012297.1">
        <property type="protein sequence ID" value="ENSLOCP00000012276.1"/>
        <property type="gene ID" value="ENSLOCG00000010039.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687962"/>
    <dbReference type="KEGG" id="loc:102687962"/>
    <dbReference type="CTD" id="503607"/>
    <dbReference type="GeneTree" id="ENSGT00940000165778"/>
    <dbReference type="HOGENOM" id="CLU_000288_5_16_1"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010039">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000061">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE CLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="159"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="86"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="484" mass="57632" checksum="601010CF611A0F09" modified="2014-04-16" version="1">MRNSKRVHSPGCWIRDDSWEEKYSSCKRRKRSHSSERENRPSRHNRHHKTSEGHYLESKSLNEKLDCKDRRGRRCSNDVSSGYDNGHLKDRDWHHYSKSSGHSGRSRRSSRPYRRRGRESFSAYRSYSRSHRRKRSRSFEDDEEGHLIYHSGDVLRARYEIVCTLGEGAFGKVVECIDHEKGGARVALKIIKNFDRYREAAVSEVEVLEQINSLDCDRKYACVRMLDWFDHHGHICIVFELLGLSTYDFLKENGFMPFPMDQIREMTYQIFRAVRFIHRSKLTHTDLKPENILFVNSDYYMEYNPRMKRDERTLKNPDVKVVDFGNATYDHEHHTSVVSTRHYRAPEVILELGWNQACDVWSLGCILIEYYLGLTLFQTHDSKEHLAMMERVLGPIPTHMLQKTRKRRYVHHDRLDWDEHSSAGRYVRKHCRPLKQYMASKSLEHEQLFDLIGKMLEYDFSKRISLDQALKHPFFDPIRKGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWU0</accession>
    <name>W5MWU0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complement C1q like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017529">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634355.1">
        <property type="nucleotide sequence ID" value="XM_006634292.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWU0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012849"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012875.1">
        <property type="protein sequence ID" value="ENSLOCP00000012849.1"/>
        <property type="gene ID" value="ENSLOCG00000010481.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687973"/>
    <dbReference type="KEGG" id="loc:102687973"/>
    <dbReference type="CTD" id="100151368"/>
    <dbReference type="eggNOG" id="ENOG502QSKV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161639"/>
    <dbReference type="HOGENOM" id="CLU_001074_3_1_1"/>
    <dbReference type="InParanoid" id="W5MWU0"/>
    <dbReference type="OMA" id="GYEMLKF"/>
    <dbReference type="OrthoDB" id="10070467at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010481">
        <property type="expression patterns" value="Expressed in brain and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF96">
        <property type="entry name" value="COMPLEMENT C1Q-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868909" evidence="7">
        <location>
            <begin position="21"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="123"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="256" mass="26998" checksum="02BFA60CC653C7D3" modified="2014-04-16" version="1">MVLVLVILIPVLVNSAGTSAHYEMLGTCRMVCDPYGTKSPASTATAETVRDHSLIQSLPTFIQGPKGEPGRPGKAGPRGPPGEPGPPGPMGPPGERGEPGRPGLPGPPGPNTAAGAISAATYSTVPKIAFYAGLKKQHEGYEVLKFDDVVTNLGNHYDPTTGKFTCSIPGIYFFTYHVLMRGGDGTSMWADLCKNNQVRASAIAQDADQNYDYASNSVVLHLEPGDEVYIKLDGGKAHGGNNNKYSTFSGFIIYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX99</accession>
    <name>W5MX99_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">G-patch domain containing 2 like</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01003756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632411.1">
        <property type="nucleotide sequence ID" value="XM_006632348.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015205986.1">
        <property type="nucleotide sequence ID" value="XM_015350500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX99"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013008"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013036.1">
        <property type="protein sequence ID" value="ENSLOCP00000013008.1"/>
        <property type="gene ID" value="ENSLOCG00000010621.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686754"/>
    <dbReference type="KEGG" id="loc:102686754"/>
    <dbReference type="eggNOG" id="KOG0154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000025698"/>
    <dbReference type="HOGENOM" id="CLU_041240_0_1_1"/>
    <dbReference type="InParanoid" id="W5MX99"/>
    <dbReference type="OMA" id="YQRDFWL"/>
    <dbReference type="OrthoDB" id="6095487at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010621">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="479" mass="53521" checksum="DD685C2EDB59E26B" modified="2014-04-16" version="1">MDELVHDLASALEQTSEQTKLEELWEEMVLSPLQQRRQIRRRRGRKRRCDSSHSLEHARCFSEASESSLDEAAKDCRESASANANAANFSDSDDMIVAKRRPSFSSSLKSKQHSWPESDSFTENTPGRPLRRRRKVKRMTSDVTVSLQEKLKVSELDSERAGSHKLAKKQRLSKVKKSACPWAGGSEPVGSSGLVHQEGWKEKMMFESEEQKEASDENMSECETSSVCSSDPGLFTNDEGRQGDDEQSDWFFEGECGPGIGVPGFLPGWETDSQPELETLECGLEKLSSPTFLQPARPAQRGYHARLNRLPGVAARCIRKGRRRLLGKDSSVASMTMERMSHFSQDPYQKDFWLPSIGKRDWHQFNPLSPLYPVDVVSEGSHRRCSSSLCKTRQTSVHLGLLCTGDIKRRRKTTAVPSSAAACIPFHEDTLGEVIEGSQSDTRSLGKGSTGGGGPVVPEKQDFIKPESFFDSQKEQEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXH3</accession>
    <name>W5MXH3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lin-54 DREAM MuvB core complex component</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXH3"/>
    <dbReference type="FunCoup" id="W5MXH3">
        <property type="interactions" value="1901"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013082"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013111.1">
        <property type="protein sequence ID" value="ENSLOCP00000013082.1"/>
        <property type="gene ID" value="ENSLOCG00000010680.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693135"/>
    <dbReference type="CTD" id="132660"/>
    <dbReference type="eggNOG" id="KOG1171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155881"/>
    <dbReference type="InParanoid" id="W5MXH3"/>
    <dbReference type="OMA" id="XKPVVVN"/>
    <dbReference type="OrthoDB" id="6283463at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010680">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="591"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="819" mass="86124" checksum="3ED552E33C86E4EC" modified="2014-04-16" version="1">MEVVSPELNSLLPEEIMDTEAIVVVEDLPGETAVPVEELEPEEGQQEAPLPMETELSESEIATLSTTLPQKVQPLGSSAPSDPALSSTNSGSQLLLATSSSPAVKSATAKLAGAKPASPALSTGLQKLTAPFTLSPNHQIILNKVAASSAEVKPATTQVIKQEGQKLVVTTIGKAGQPIVLALPHTSPKPGQAQTGGEVKAQSQQFKVVTIGSRTDLKPVVGVQAMSSASQLLGTSQHSPLQAQQLKTVQIAKKTQTSSTGPMITKFIITKPVNSKGVSGQTSVSPVIAGRVLSQNPPGTPPRTIAISDSVVSATLNTTPQSISSSKIAISPLKSPSKLTVVSMASQSPSSPQKPVGVPINVALGQPILAVQQSAPSSPAKVVSNHSAPQTVKPVQTVAVGGVGTPQFKTIFPLATSPNVQQIQVPGSRFHYVRLVTASTASSSPQTGSPSTNSHTLQTAKPVMVNTTPVRMSVPIVPAQTVKQVVPKPLNATSQIVTTSQAQQRLIMPATPLPQIQPNLTSLPPGTVLAPAPGTGNVGYAVLPAQYVTQLQQSTFVSLASNTSFAASAGIQTQARLPLNGLSPSDSASRPRKPCNCTKSQCLKLYCDCFANGEFCNNCNCTNCFNNLEHETERLKAIKACLDRNPEAFKPKIGKGKEGESDRRHSKGCNCKRSGCLKNYCECYEAKIMCSSICKCIGCKNFEESPERKTLMHLADAAEVRVQQQTAAKTKLSSQISDLLTRTTPAITSGGGKLPYTFVTKDVAEATCECLIEEAERAELSHRPQAVAERMILEEFGRCLMRIINSAGKAKTDPCSINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXQ6</accession>
    <name>W5MXQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Heterogeneous nuclear ribonucleoprotein D</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01013379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627096.1">
        <property type="nucleotide sequence ID" value="XM_006627033.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193776.1">
        <property type="nucleotide sequence ID" value="XM_015338290.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXQ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013165"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013193.1">
        <property type="protein sequence ID" value="ENSLOCP00000013165.1"/>
        <property type="gene ID" value="ENSLOCG00000010743.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682218"/>
    <dbReference type="KEGG" id="loc:102682218"/>
    <dbReference type="CTD" id="3184"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158010"/>
    <dbReference type="HOGENOM" id="CLU_012062_1_1_1"/>
    <dbReference type="InParanoid" id="W5MXQ6"/>
    <dbReference type="OMA" id="YIPDYNG"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010743">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12583">
        <property type="entry name" value="RRM2_hnRNPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000156">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012956">
        <property type="entry name" value="CARG-binding_factor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF3">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08143">
        <property type="entry name" value="CBFNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="53"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="138"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="35024" checksum="BCF0FF2A5E0F8306" modified="2014-04-16" version="1">MSEEHQVGEDPVVKMEEDGEAGGEEQQMATGESGASESEGSKIDASKNEEDEGKMFVGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPMTGRSRGFGFVLFKDADSVDKVIVQKEHKLNGKVIDPKKAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMENKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQWGGRGNFVSRARGRGGPSQNWNQGYSSYWNPGYSNYNYGYNSQGYGGYGGYDYSGYNNYYGYGDYSNQQSGYGKTPRRGGHQNSYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZJ1</accession>
    <name>W5MZJ1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L34</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01031590">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZJ1"/>
    <dbReference type="FunCoup" id="W5MZJ1">
        <property type="interactions" value="1303"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013800"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013829.1">
        <property type="protein sequence ID" value="ENSLOCP00000013800.1"/>
        <property type="gene ID" value="ENSLOCG00000011235.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008294"/>
    <dbReference type="HOGENOM" id="CLU_118652_0_1_1"/>
    <dbReference type="InParanoid" id="W5MZJ1"/>
    <dbReference type="OMA" id="RCHKCVR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011235">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46595">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="126" mass="14553" checksum="032AF65C3AE18041" modified="2014-04-16" version="1">IYFYLFRINMVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLRGVRAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1U4</accession>
    <name>W5N1U4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036214">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036215">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1U4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014603"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014632.1">
        <property type="protein sequence ID" value="ENSLOCP00000014603.1"/>
        <property type="gene ID" value="ENSLOCG00000011879.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RB0C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="HOGENOM" id="CLU_010468_5_1_1"/>
    <dbReference type="InParanoid" id="W5N1U4"/>
    <dbReference type="OMA" id="KSITHEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001809">
        <property type="entry name" value="Si:ch1073-365p7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AIG1-type G" evidence="16">
        <location>
            <begin position="9"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="16">
        <location>
            <begin position="236"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="16">
        <location>
            <begin position="427"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="613" mass="67626" checksum="52019F75DFCDBAF8" modified="2014-04-16" version="1">LQYVACVPPTATRLVLLGKTGSGKSRSGNTILGREEFLWLASANSVTQQCDRITGEINGRRVSVVDTPGVFDTLQSHEDVKREILRCMALSAPGPHAFLLVVKLGRQTEEEREAVRRVLAQFGAEALRYTLVLFTHGDHLKGDIRAFVKSNEHLTCCGCSFLTLDPDSQLRHNFLLAKSNTFHRKLLTVVENITAGASPLQHRHTLTPALPASAGSGIDIEAHFPTCFTDAALQDPSGLKIFLLGRTGVGKSASGNTILGPGHFRSKPRAELVTRECELGTGEVAGRPVCVLDTPDTQLLNEEHTLQNIRGVFLRAQEVHALLLVLPVGDARDTHRGAQEMIQDLFGPQALRYTIVLFTRGEDLEGETLQEYVQSQSRELQSLVEQCGGRTHVFCNKNTADRSQVAQLLDKIEALKSQAKPNQMSEPRDLRIVLLGKTGCGKSRTGNTILKQDVFQHECSPKSITHECQWGTGTVEGRKVTVVDTPGFFDTELPQGILRREIGRCIAMSSPGPHAFLLVIQVGRQTPEEKRVVSEILRLFGQKALRFALVLFTHGDSLGKGTTIEEFFERNDHLTGLIEKCGGRYHIFDNEDKSSRTQVRQLLAKIEAMVQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2G0</accession>
    <name>W5N2G0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CDGSH iron-sulfur domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca(2+) stores during autophagy.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="14">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Mitochondrion outer membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the CISD protein family. CISD2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2G0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014848.1">
        <property type="protein sequence ID" value="ENSLOCP00000014819.1"/>
        <property type="gene ID" value="ENSLOCG00000012043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156660"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012043">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.90:FF:000001">
        <property type="entry name" value="CDGSH iron-sulfur domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045131">
        <property type="entry name" value="CISD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019610">
        <property type="entry name" value="FeS-contain_mitoNEET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680:SF33">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10660">
        <property type="entry name" value="MitoNEET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="15">
        <location>
            <begin position="84"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="138" mass="15749" checksum="E71591405EFCD1B7" modified="2014-04-16" version="1">TFKMVLESIARIIKVQLPAYLKRLPLPETVGGFAKLTVSEWLRLLPLLGILALLGYLTIRPFLPKKKKQKDSLINLKIQKENPKVVNEIDIEDLKSTNACYCRCWRSKTFPVCDKSHIKHNELTGDNVGPLILKKKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2T6</accession>
    <name>W5N2T6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MyoD family inhibitor</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016674">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2T6"/>
    <dbReference type="FunCoup" id="W5N2T6">
        <property type="interactions" value="1098"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000014974.1">
        <property type="protein sequence ID" value="ENSLOCP00000014945.1"/>
        <property type="gene ID" value="ENSLOCG00000012155.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691925"/>
    <dbReference type="KEGG" id="loc:102691925"/>
    <dbReference type="CTD" id="4188"/>
    <dbReference type="eggNOG" id="ENOG502RZMC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160187"/>
    <dbReference type="HOGENOM" id="CLU_067479_1_0_1"/>
    <dbReference type="InParanoid" id="W5N2T6"/>
    <dbReference type="OMA" id="CEFMTFC"/>
    <dbReference type="OrthoDB" id="8958154at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012155">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030111">
        <property type="term" value="P:regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF1">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="249" mass="26287" checksum="4C997C9F17BC3407" modified="2014-04-16" version="1">MSGARGPPASSPDSPEDEDHCSIQHQPGAPVSEEEAFENCEQSHAEATPREPPVPHGIERNTNDDRTPLKMDSSTPSKSITCQPQASPFSPTLDGLAPETPCLLHNGGQRPPPPQKNGSPQGRPAAPRKPHGKLQSHPSLSSQSSKRSKGSSKSASSQIPIEAEDDCCVHCILACLFCEFLTLCNIVLDCTTCGSCASDDSCFCCCCGSEECGDCDLPCDMDCGIIDACCESADCLEICMECCGLCFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3R4</accession>
    <name>W5N3R4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ras-related protein Rab-15</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB15 may act in concert with RAB3A in regulating aspects of synaptic vesicle membrane flow within the nerve terminal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">The GTP bound form of RAB15 interacts with REP15. Interacts (GTP-bound form) with MICAL1, MICAL3, MICALCL, EHBP1 and EHBP1L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01019056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206151.1">
        <property type="nucleotide sequence ID" value="XM_015350665.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3R4"/>
    <dbReference type="FunCoup" id="W5N3R4">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015273"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015302.1">
        <property type="protein sequence ID" value="ENSLOCP00000015273.1"/>
        <property type="gene ID" value="ENSLOCG00000012406.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688580"/>
    <dbReference type="KEGG" id="loc:102688580"/>
    <dbReference type="CTD" id="376267"/>
    <dbReference type="eggNOG" id="KOG0078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157848"/>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="W5N3R4"/>
    <dbReference type="OMA" id="SSKNCWC"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012406">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd04117">
        <property type="entry name" value="Rab15"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ras-related protein Rab-15 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041826">
        <property type="entry name" value="Rab15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="24539" checksum="274120F44C76D294" modified="2014-04-16" version="1">MAKQYDVLFRLLLLGDSGVGKTCLLCRFTDNEFHSSHISTIGVDFKMKTLEVDGIKVRIQIWDTAGQERYQTITKQYYRRAQGIFLVYDITSERSFQHIMKWVSDVDEYAPEKVQKILVGNKSDEEDKRQVATEQGNKLAKTYGMDFFETSACTNHNIKESFTRLTELVLQAHKKELDGLRASINDELYIADLEDDDNKRDRSVNSQKACWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3Y3</accession>
    <name>W5N3Y3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">G protein-coupled receptor 37 like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01016612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3Y3"/>
    <dbReference type="FunCoup" id="W5N3Y3">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015342"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015371.1">
        <property type="protein sequence ID" value="ENSLOCP00000015342.1"/>
        <property type="gene ID" value="ENSLOCG00000012462.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286942"/>
    <dbReference type="HOGENOM" id="CLU_029119_0_0_1"/>
    <dbReference type="InParanoid" id="W5N3Y3"/>
    <dbReference type="OMA" id="DSCVMKP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012462">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000059">
        <property type="entry name" value="G protein-coupled receptor 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003909">
        <property type="entry name" value="GPR37_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216:SF4">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 37-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216">
        <property type="entry name" value="PROSAPOSIN RECEPTOR GPR37 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01421">
        <property type="entry name" value="GPR37ORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="115"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="450" mass="49173" checksum="CC18D6CB1A152DB7" modified="2014-04-16" version="1">KRVPRGAKDGSPRKRDQQFPFSYRNPRPFDPNDFFTTPKTQQLSSTFRPAVSGAGTPLDLFKDGSPEAEVSGNITSAEKGTTRIHNPLYPVTDSSYSAYAVMFLSLIVFAVGIVGNLAVMCVVWHNYYMKSAWNCILASLAFWDFLVLFFCLPVVIFNEITKTRLLGDLSCKTVPYLEMTSLGVTTFSLCALGIDRFHAATSTQPKTRPVEQCQSILAKLAVIWVGSMILAVPELLLWQLGQDASPVTGALADSCTMRPSASLPESVYSLVLTYHDARMWWYFGCYFCLPMLFTLTCQLITRRISGTARKQESRGSPRKGGPCDGQLNCTVVALAVVYGACVLPENVCSIVLAYLSSEVSRHTAALLALVNQFFLFVKSAVTPVLLLCLCRALGQAFMDCCCCCCEDCGPPERGAARPSASKLKTTEVSSSIFFDKPRDSASVLALGTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>W5N432</accession>
    <name>W5N432_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein engrailed-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Engrailed homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006634501.1">
        <property type="nucleotide sequence ID" value="XM_006634438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N432"/>
    <dbReference type="FunCoup" id="W5N432">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015391"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015420.1">
        <property type="protein sequence ID" value="ENSLOCP00000015391.1"/>
        <property type="gene ID" value="ENSLOCG00000012504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683601"/>
    <dbReference type="KEGG" id="loc:102683601"/>
    <dbReference type="eggNOG" id="KOG0493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159935"/>
    <dbReference type="HOGENOM" id="CLU_051739_0_0_1"/>
    <dbReference type="InParanoid" id="W5N432"/>
    <dbReference type="OMA" id="HAHRVTN"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012504">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060561">
        <property type="term" value="P:apoptotic process involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060385">
        <property type="term" value="P:axonogenesis involved in innervation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cell fate specification"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021555">
        <property type="term" value="P:midbrain-hindbrain boundary morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000167">
        <property type="entry name" value="Homeobox protein engrailed-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050720">
        <property type="entry name" value="Engrailed_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000747">
        <property type="entry name" value="HD_engrailed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019549">
        <property type="entry name" value="Homeobox-engrailed_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019737">
        <property type="entry name" value="Homeobox-engrailed_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341">
        <property type="entry name" value="HOMEOBOX PROTEIN ENGRAILED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN ENGRAILED-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10525">
        <property type="entry name" value="Engrail_1_C_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00026">
        <property type="entry name" value="ENGRAILED"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00033">
        <property type="entry name" value="ENGRAILED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="175"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU510713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="29372" checksum="C3CF4C70E36DBBE4" modified="2014-04-16" version="1">MEENDHSNREADPQESGDESNRAIIPLLQAPGNLQLPHRITNFFIDNILRPDFGRRKEVTFSRDQSHASRRENLTPSAPGTGQVGGTVAGEGSSNPHPVNGAKKGDIAIEEPLNSRGESGEQCLSSDSDSSQASSTTPSQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKTASKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKASGSKNSLALHLMAQGLYNHATTPKDDKSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4B8</accession>
    <name>W5N4B8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat domain 23 like</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14485:SF4">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41627" checksum="3C651A688B8EFEBF" modified="2014-04-16" version="1">LTPAEKLDSARTACEQLIKSHEVSRAMTELIRCVALSRLVYGDGHWRLAEAFANVAYGYLKLRGLPAQAQQHAETARDILLAGTLLPELVEKKDILPTLMTTYYTLGVAHLITLNEAHQNLQKAEKIYQDIVRQNSQNKASVLTNVYRVYYQLVHRVALQQKRPASAVLYFEKAASCVSSTRGQDSLDLVGIYQEMARAEQMRARHEEAIEHLLQAHSIATAQLDESVETAQAALLLAQGYAAAGSLRSSELTEQYFKESINMFRSVLGEENPLTFPAVDNYTSWLIQTGRQQQALELLRSTLQRRVEAFGNYSDSMADTLSLMSSITLAGGEMKKAYKLLKKCLEIQTVLYGSQHKKSRQTQQLLDMLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6V8</accession>
    <name>W5N6V8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(+) ADP-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01002311">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6V8"/>
    <dbReference type="FunCoup" id="W5N6V8">
        <property type="interactions" value="765"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016367"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016397.1">
        <property type="protein sequence ID" value="ENSLOCP00000016367.1"/>
        <property type="gene ID" value="ENSLOCG00000013281.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157664"/>
    <dbReference type="HOGENOM" id="CLU_018325_0_0_1"/>
    <dbReference type="InParanoid" id="W5N6V8"/>
    <dbReference type="OMA" id="RSPTTWM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd09518">
        <property type="entry name" value="SAM_ANKS6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing 6"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="8">
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            <begin position="122"/>
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        </location>
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    <feature type="repeat" description="ANK" evidence="8">
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            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
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            <begin position="331"/>
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        </location>
    </feature>
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            <begin position="365"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="798"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="204"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="509"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="875"/>
            <end position="913"/>
        </location>
    </feature>
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        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
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            <begin position="434"/>
            <end position="445"/>
        </location>
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            <begin position="551"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7A4</accession>
    <name>W5N7A4_LEPOC</name>
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        <submittedName>
            <fullName evidence="1">Nicolin 1</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG5"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Nicolin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31239">
        <property type="entry name" value="NICOLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31239:SF2">
        <property type="entry name" value="NICOLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="218" mass="25032" checksum="08219F9FB2DCEACF" modified="2014-04-16" version="1">FLKMSEHTVECTVKRPVALQMGDVKTDMSRPGIFVIDVTFPQGQTVNIQEISFKNYYTAFLTVRLQRQDPESPENALKWITGLKDCCLMPNPHTERGSQDYFSIYRQQMLIEPDDVTSVRLILRQPSSSWLTFTLEEIKIFECVNEDSKNNFPSWFSNLSPVDQSPDLQEGIPDPKKVSSSIQQMWALTEVMQTNQTAARIGRFDVDGCYEINLLSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N879</accession>
    <name>W5N879_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="AHAT01015145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N879"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016838"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016868.1">
        <property type="protein sequence ID" value="ENSLOCP00000016838.1"/>
        <property type="gene ID" value="ENSLOCG00000013656.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157877"/>
    <dbReference type="HOGENOM" id="CLU_000288_81_9_1"/>
    <dbReference type="InParanoid" id="W5N879"/>
    <dbReference type="OMA" id="CHGRENT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013656">
        <property type="expression patterns" value="Expressed in heart and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001037">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000770">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF24">
        <property type="entry name" value="SRSF PROTEIN KINASE 3-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="591" mass="67286" checksum="CAFF445034B591EE" modified="2014-04-16" version="1">KQEDPSEYCYGGYHPVTIGDTFNGRYRVLRKLGWGYFSTVWLCLDLHKKRVVAVKVSKSGANFTQAAQDELALLRCTCGSMRKHPYGECIVELLDDFRLVGKNGIHMCLVFELLGQNLHSWMMDYTSLGLPLSWVKSIIRQVLQGLDYLHTQCKIIHTDIKPENILLCIGEQCTKQSEEGAVQDKLHEYKRMDRAGTSLKYCNINVVAVNTRKLRFPKPVLNTVGTKQVCFSTVCMCVWEGPAFITFHVGRKYLHKDSSEIEDVRISHPYNVNGSFSMLNGISFLKIRVTIINENKDLACITKIPVSYIWWCPANSMPPLRAHKQNCHCWHLKKGSIDYSFQCALRVPNLVKLQRILNLSPSFFYFHLVFSGVMINGGVLHLLCIPNLRDPLPLPPYSLSCLPPSSPDVDHCDNLQFDPKSVTAEEIKVKIADLGSSCWVYKHFSDEIQTRQYRSLEVLIGSDYGPPADIWSTACMAFELVTGDSLFEPRAGKTYSVEEDHLAHIIELLGKIPARVALSGKYSREYFTYKGDLRRIRVLRPWGIYEVLVEKYHLGLKEAALFADFLLCMLAFSPEQRATAVQCLRHPWLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8B4</accession>
    <name>W5N8B4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8B4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016873"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016903.1">
        <property type="protein sequence ID" value="ENSLOCP00000016873.1"/>
        <property type="gene ID" value="ENSLOCG00000013687.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQXA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155368"/>
    <dbReference type="HOGENOM" id="CLU_014825_0_1_1"/>
    <dbReference type="InParanoid" id="W5N8B4"/>
    <dbReference type="OMA" id="WDKYKRK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013687">
        <property type="expression patterns" value="Expressed in ovary and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF7">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE TIPARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="5">
        <location>
            <begin position="208"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47757" checksum="D5824F1D1414C7F6" modified="2014-04-16" version="1">YHTHPEPDTPICVAFLHGTCQFTAGCPRHHTLLPYHWQLRWAESGRWESLPPLLQELLERLYCNPEHHHVMLLHQSVLMTVDMESMTVQPPLPFTALRRLSTRGVLGGPLHIPYTFYWWDDPCWREYQVGAGRTAGGAPSQGAKEAKPEAAASQQPAVQESQGMRQKARKLRPVFRALVTMLPHLRSFPETCFWGSPTGLPPMQDSQYPTTWASMDPSMDFIQVNLSLDDRTYLIIHQLFHHTLPELKYQLLSVSRVQNRFLWDKYKRKKEYMCRNPVEEEQRGHERHLFHGTAGEAVRGICKHGFDPRLSGKHAAVFGLGTYFTRSASLANRYALPDPQGAHYLFLSKVLVGRWAQGKASLRRPPALQPDNPASDLYDSCVDRVADPSIYILFDSDQCYPYFLLTYRKVEPVIVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8D7</accession>
    <name>W5N8D7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006632675.2">
        <property type="nucleotide sequence ID" value="XM_006632612.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206583.1">
        <property type="nucleotide sequence ID" value="XM_015351097.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069049532.1">
        <property type="nucleotide sequence ID" value="XM_069193431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8D7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016896"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016926.1">
        <property type="protein sequence ID" value="ENSLOCP00000016896.1"/>
        <property type="gene ID" value="ENSLOCG00000013705.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690200"/>
    <dbReference type="KEGG" id="loc:102690200"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244937"/>
    <dbReference type="HOGENOM" id="CLU_009579_11_3_1"/>
    <dbReference type="InParanoid" id="W5N8D7"/>
    <dbReference type="OMA" id="YRNGNEF"/>
    <dbReference type="OrthoDB" id="6358729at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013705">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF32">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="73"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="467" mass="51970" checksum="50C8DA206969E144" modified="2014-04-16" version="1">MMSSTSSTGIFSSLSTVTLDAALHSSLMALHLNSSHISNHSSDGMEPLNSSEQMEKNWPALLFLIIIILTIGGNILVILAVSLEKKLQNATNFFLRSLAVADMLVGILVMPVSLINILYGNVWPLPHKLCPIWIYLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAMMKIAAVWTISIGVSMPIPVIGLHNEDKVFVNGSCVLNEDNFILIGSFVAFFIPLIIMVVTYCLTVQVLQRQANVFLYEGHPKQPRRSSLNCLKKDTSQTPSDNISIIQSSEGASQLNSPVNRDPGHGRKGMMQAIKNERRASKILGVVFFLFLLMWCPFFITNVMNVLCKNACNTHLLEELLNVFAWVGYVSSGINPLVYTLFNKTYRQAFSNYICCQYRNNKKAFMNVYQAPVATPTMYSKDVNLNSNCRNGNELNSMDLDDTDEALEMQPGTSEVSINSCNVTSDHVSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5N929</accession>
    <name>W5N929_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Microtubule associated scaffold protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MTUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002189">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N929"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017138"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017169.1">
        <property type="protein sequence ID" value="ENSLOCP00000017138.1"/>
        <property type="gene ID" value="ENSLOCG00000013895.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPVG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183026"/>
    <dbReference type="HOGENOM" id="CLU_280007_0_0_1"/>
    <dbReference type="InParanoid" id="W5N929"/>
    <dbReference type="OMA" id="NITANCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013895">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051293">
        <property type="entry name" value="MTUS1/CCDC69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200:SF7">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED TUMOR SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200">
        <property type="entry name" value="TOUCAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="294"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="625"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1090"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="789"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="952"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1017"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="658"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1092"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1105"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1124" mass="121969" checksum="3DC3E05E915A7CCC" modified="2014-04-16" version="1">TNLVRKSLNITANCKIGDRNGNKVLCNTESLPDSFRSISSLSSGSTGSPDVEMIECDLKSAGCSMSSQESMENSPQNHLGMELDTWNDNLALETSGFQEYSSGQKWLEDAPETENPGCANEEENISVGTGQSSLDDPSSGELLVRRSSFDASGSDKPSSLSVVEESSSSCSVPADLGRLSSVMPDICGGSLEGRLSHVRWKGLNQDCTAQETCGETGVDAADRGSVWASAVEVKGPEQLQSILLPPKGDSAAESKTFLVISSEDSDCNCSVQTSTPQPDSRNVTFCVHSFDNDVSEQPSDIREGDLGSPAHRIVQQRPPSPKEPTRTKPSALVASKNAKAEIKKFPKPDFKNIKPKVVSRPVTLSKTSSSARAKASPRPAVASRGPPSLSPPRDVPQTGQKNSGSGSWKSRNNCVQKTADRDGCANSSSSPTSDPAVGGGTAKHPSKQGSQNKPAASSPKSSPRKERTGCGLLSSVVPEAAQNRISPESPPDDVRERSALDFQALISQRVRASRSSELQIFTGPLSPEQSAAPDASRTRLGQKPYPSKPRGAPSSLAPAANLRLPPPASKPKLGASGRDGSAPGNASPSRTKPLSAPDVRNLRIPLRTGAETVFFPVASALSSARSAAASKLPVKPQALRRTSSVSSVASSLSEPSTATGNGRGSKTSAFQAQNPPRATSTSQTPKSSSNVSSRQVRPTDRPLQTSLNISPASTETVTPGVSVDRARQKSPRDRPAQAAGPPDPLPAESRALGVAHYRTQCERSGERAERLRALLAASDSRFEAVALVVQHVLAQREEALKQRKELSQELVNLRSELVGTASSCERLERERDELRGAYEALLQRLQEQHRAELAELEERLRGFYAAEWEKVHEAYQREADKIKAQMQQQVDELGSKHESLRKELETRHSEKIELLKQHYETSIEDLKISQDLEKQSLNKSFQDTESSLCEQIHELTAANDSLNEKLKAEEERRRALMDKNQRDSHTLYLEQELESLKVVLDIKTEQLHQQDKKLIQMEKLMERNIKLEEGLNRVQQENEDLKARMDKHAALSRQLSTEQAVLQESLEKESKVNKRLSMENEELLWKLHNGDLSSPRRLSPSSPSGVFQSPRNSGAFSSPPVSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9F8</accession>
    <name>W5N9F8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 17">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="17">ATase</shortName>
            <ecNumber evidence="3 17">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 17">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:14907"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="20">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 17">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 17"/>
    <dbReference type="EMBL" id="AHAT01002212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9F8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017267"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017298.1">
        <property type="protein sequence ID" value="ENSLOCP00000017267.1"/>
        <property type="gene ID" value="ENSLOCG00000014005.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003428"/>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_1"/>
    <dbReference type="InParanoid" id="W5N9F8"/>
    <dbReference type="OMA" id="IRHFGVK"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014005">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000047">
        <property type="entry name" value="Amidophosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 20" id="KW-0408">Iron</keyword>
    <keyword evidence="12 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="21">
        <location>
            <begin position="13"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="511" mass="55419" checksum="D0CC9781D8DF995A" modified="2014-04-16" version="1">AMEFEELGIREECGVFGCVAAGEWPTELEVAQVITLGLVALQHRGQESAGIVTSNGASPPVYYSHKGMGLVSNAFPPEALQKLEYGNLGIGHTRYSTTGISELQNCQPFVVETLHGKIAVAHNGELVNAQKLRKQVMRHGVGLSTSSDSELIMQLLALTPPMEELDTPDWVARIKNLMTETPTSYSLLVMHQDVIYAVRDPYGNRPLCLGRLVPSSRLQGAGGETGSAETEGWVVSSESCGFQSIGARYYREVLPGEIVQISKHGVKTLSVVPRPEGDPPAFCIFEYVYFARPDSIFEGQMVYSVRQRCGRQLAIEAPADADVVSTVPESATPAALGFAQQAGLPYVEVLCKNRYVGRTFIQPNTRLRQLGVAKKFGALTDNFAGKRVVLVDDSIVRGNTISPIIRLLREAGAKEVHIRVASPPIRFPCYMGINIPTKEELIANRPEFENIARYIGATSVVYLSVEGLVAAVQGGVASREEKDAVIGGKASRKGGHCTACLTGKYPVELEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9H9</accession>
    <name>W5N9H9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-glutamate cyclase</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01022146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9H9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017288"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017319.1">
        <property type="protein sequence ID" value="ENSLOCP00000017288.1"/>
        <property type="gene ID" value="ENSLOCG00000014018.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002237"/>
    <dbReference type="HOGENOM" id="CLU_033607_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9H9"/>
    <dbReference type="OMA" id="HIGHPGL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014018">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1640.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1640.20:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2040.10">
        <property type="entry name" value="PSTPO5379-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.20">
        <property type="entry name" value="TON_0340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025504">
        <property type="entry name" value="GLUCM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14336">
        <property type="entry name" value="GLUCM-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate cyclase-like C-terminal" evidence="3">
        <location>
            <begin position="304"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="624" mass="67785" checksum="CB380850A90AAF99" modified="2014-04-16" version="1">IYCSMITFTPYILELTSSLSTVFRELARGRKLGIRGASGMTEGYKKASIVILHRDLADDFEAFCHANSGPLPLLYRSQPGELTCPVLTHASDVRTLYPEYFKYEQGNLTSRLPSLLDYSEELANMVTFYLGTAMSDHQKLQRAEVPPRPGDKKHAVSIYKTSVPCCGVGNFQCDLRVFMKPMPERNLDTHLNHSLHGVPVHIGHPGLIGVEDLRRPDNGDAMEVQPGDVPVFWACGTTGAEAVRSSKTPLSFTCSTDLSSYEDGASPLARSLAPSETPLAFCISQQPLHYSLASAAAVQRIRALETHIGADPGERGIKALLVPEELLKASLSLSHAASVLITTGFPTHFTHNPPEENDGPPGAIAMAAMLQALNKRVTIVTDHRALDMNRKIIEDAVEQGILKTPVPVLSFQNSGADSVLGFLCHNGDPVKPRYDHLVAIERAGMAADGNYYNARKVNIKHLVDPIDILFTTASSIPGITTTGIGDGGNELGMGKVREAVRKHVPNGELIACDVAADFAITAGVSNWGGYAVACALYVLNHCPVHERYLRRAVGLPRNIQHSSWTAALPTVRKEEKILEILVKYGIRSGRTGTLGMEVDGLPFHSIHSAMIQRLIDMTLHQAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA50</accession>
    <name>W5NA50_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="7">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="function">
        <text evidence="4">Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex. The branched-chain alpha-ketoacid dehydrogenase is a large complex composed of three major building blocks E1, E2 and E3. It is organized around E2, a 24-meric cubic core composed of DBT, to which are associated 6 to 12 copies of E1, and approximately 6 copies of the dehydrogenase E3, a DLD dimer. Interacts with PPM1K.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the BCKDHA family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01029507">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029510">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NA50"/>
    <dbReference type="FunCoup" id="W5NA50">
        <property type="interactions" value="1406"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017509"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017541.1">
        <property type="protein sequence ID" value="ENSLOCP00000017509.1"/>
        <property type="gene ID" value="ENSLOCG00000014217.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1182">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063174"/>
    <dbReference type="HOGENOM" id="CLU_029393_1_2_1"/>
    <dbReference type="InParanoid" id="W5NA50"/>
    <dbReference type="OMA" id="GMFRGVN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014217">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160157">
        <property type="term" value="C:branched-chain alpha-ketoacid dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000151">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000172">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="9">
        <location>
            <begin position="70"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="9">
        <location>
            <begin position="269"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="382" mass="43794" checksum="4777FC81CCCB1A4B" modified="2014-04-16" version="1">AVLRQQTFDSLDEKPQFPGASADFVDHLEFIQPNVISGIPVYRVMDRQGQIINPAEDPQLPKEEVLNFYQKMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHIGSAAALSPNDLVFGQYREAGVLMYRGFPLDLFMAQCYANADDLGKGRQMPVHYGSKDLNFVTISSPLATQIPQAAGAAYAVKRENANRVVICYFGEGAASEGDAHAGFNFSATLECPVIFFCRNNGYAISTPTYLSVLFSNGNSASERHISAGVSRSPSPRFRRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYMTARGWWGEEEERAWRKQSRRRVMEAFEKAERRLKPHPELLFTDVYHDDPPHLQRQREAMWRHVQTYKEHYPLDQYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB03</accession>
    <name>W5NB03_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aminopeptidase</fullName>
            <ecNumber evidence="17">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01005017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB03"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017812"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017844.1">
        <property type="protein sequence ID" value="ENSLOCP00000017812.1"/>
        <property type="gene ID" value="ENSLOCG00000014462.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154876"/>
    <dbReference type="HOGENOM" id="CLU_003705_2_2_1"/>
    <dbReference type="InParanoid" id="W5NB03"/>
    <dbReference type="OMA" id="DAWIETS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014462">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000006">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000012">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF259">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 17" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="20">
        <location>
            <begin position="76"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="18">
        <location>
            <begin position="304"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="19">
        <location>
            <begin position="606"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="956" mass="109125" checksum="650A2E68AB0DECA6" modified="2014-04-16" version="1">GSRDADMSKGFYISKAFAVAGLVVTASAAASIVVMVIVFQQQILRRPPTPPSFPATTRAPTVDTRDMMRLPSSLRPESYRVVLQVHLFPDPNSTFHFTGNSTVQFRCLQPTRQIVLHSHKLNLTQLASQYALLTGHTANTPRVVQSILRPETQFLEILLDRELEEGKTYELYTQFAGDLADDLTGFYRSEYTENGEQRYIAATQMQPTDARRVFPCFDEPAMKAVFNITLIHPKETKALSNTASQTTQNNDYFITEFIPTPRMSTYLLAFVVCDFDYFEIGPNMKITLKIWARPDAINAGHAAYAGNITLKIVEFFEDYYGVPIPLSKLDQIALPDFGAGAMENWGLITYRETALLYDPEVSSTGNKEWIATVISHELAHQWFGNLVTMKWWNDLWLNEGFATYVSYLGANHIEPTWNIKDLMVLNEIQSVFQVDSLATSHPLSSREVDVNTPDEISELFDSITYSKGAALLRMMSEFLSEKVFRAGTKTYLEAFKYNTTVNKNLWEHLQKAADEHKLQLKVGVEAIMRTWTEQMGYPVVTINTTDGRASQQHFLIDPETAPPQPSKAGYKWHIPMTLMKSGKQGQKMLFYETGSFPDFQCKDTEWLLANIDCAGYYRVNYDSNNWNKLLQQLENSHTVIPVINRAQLIDDAFNLARAKHINTTLALDTTKFLSNETEYIPWQSALNNLDYFFLMFDRSEVYGPLQAYIRKQVIPLYNYFENITANWTRIPDNHIDQYNQVNAISVACSNGVPECQEMASGLFKQWMMNSSANPIHPNLKSSVYCSAIAAGGEREWNFAWEMFQNTTVASERDKLRYALSCSRKIWILSRYLEYTLDPEKIRKMDTTSTINYIARNVAGQSLAWDFVRAKWSFLHGEYGGGIMSFGSLIDGVTQRFSTEFELEELENFKRQHEETGFGSAARALEQALERTKANIRWVKQTKASVLAWFRRESGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBW3</accession>
    <name>W5NBW3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023831">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBW3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018122"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018154.1">
        <property type="protein sequence ID" value="ENSLOCP00000018122.1"/>
        <property type="gene ID" value="ENSLOCG00000014720.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165970"/>
    <dbReference type="HOGENOM" id="CLU_050803_1_0_1"/>
    <dbReference type="InParanoid" id="W5NBW3"/>
    <dbReference type="OMA" id="GSTHRIW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014720">
        <property type="expression patterns" value="Expressed in zone of skin and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13243">
        <property type="entry name" value="PH_PLEKHG1_G2_G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000019">
        <property type="entry name" value="Pleckstrin homology domain-containing family G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000132">
        <property type="entry name" value="pleckstrin homology domain-containing family G member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043324">
        <property type="entry name" value="PH_PLEKHG1_G2_G3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924">
        <property type="entry name" value="FI17866P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="207"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41915" checksum="BF280CFD94388569" modified="2014-04-16" version="1">KLSRLDRVVLEIVETEQAYVRDLKSIVEDYLGCIIDCGDLPLKPEEVSTLFCNIEDIYEFNSELLEDLERCSQAAAIAECFVERSEAFDIYTLYCMNYPNSVAVLRDCMKNNSLVRFFQERQATLSHSLPLETYLLKPVQRILKYHLLLQELSKHFDKSEPGYEVVEEAIITMTAVAWYINDMKRKQEHAVRLQEIESLLLNWNGPDLSGFGELVLEGSFRVQRVKKERAFFLFDKMLLIAKKRNENFVYSTHIFCCNLLLAENLKDPLSFKVSDQTIPKQQHIVQAKNQEEKRLWLHYLKRLIVENHPASLPQKARQVLGDSCCPCEYCCCSFFDHEPSKKPAASPRLDDSRGYHRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCA8</accession>
    <name>W5NCA8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutaminyl-tRNA cyclotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01023888">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627681.1">
        <property type="nucleotide sequence ID" value="XM_006627618.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCA8"/>
    <dbReference type="FunCoup" id="W5NCA8">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018267"/>
    <dbReference type="MEROPS" id="M28.979"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018299.1">
        <property type="protein sequence ID" value="ENSLOCP00000018267.1"/>
        <property type="gene ID" value="ENSLOCG00000014833.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688147"/>
    <dbReference type="KEGG" id="loc:102688147"/>
    <dbReference type="CTD" id="561142"/>
    <dbReference type="eggNOG" id="KOG3946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003107"/>
    <dbReference type="HOGENOM" id="CLU_045003_1_0_1"/>
    <dbReference type="InParanoid" id="W5NCA8"/>
    <dbReference type="OMA" id="TPFPSFW"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014833">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF3">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="15">
        <location>
            <begin position="151"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="388" mass="42583" checksum="35667575ACC6E1E4" modified="2014-04-16" version="1">MSRGGRRYRAAPVSGGSLSRWEPGRLPRARALLCGLCGLLAVAGLLGVYLSSDTPGDLRVGSADWTTDRLAHKPRKLTAGQLRSLTARVDRRRQWDALLRPILTERRPGTAGSQAVRQHISSVLGSLSAGWTVEVDSFTAPTPLGQLTFSNVLAVLDPAAPRRLLLACHYDSKLLPADSRGRVFVGASDSAVPCAMLLELASALDAQLRALRQQRPALTLQLVFFDGEEAFQEWTPTDSLYGSRHLAERMAATAHPPGSAGTSQLDAIDLFVLLDLIGAPDPLFVNHFDNTARWFDRLIAAEKRLHKLGLLASHPLEQSYFRKDVYLGPVEDDHVPFLRRGVPVLHMVATPFPSFWHTLEDSEEQMHPPTVDNLTKILAVFLAEYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NCB9</accession>
    <name>W5NCB9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TBC1 domain family member 2B</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004903">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004904">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004905">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NCB9"/>
    <dbReference type="FunCoup" id="W5NCB9">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018278"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018310.1">
        <property type="protein sequence ID" value="ENSLOCP00000018278.1"/>
        <property type="gene ID" value="ENSLOCG00000014842.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157737"/>
    <dbReference type="HOGENOM" id="CLU_011278_0_0_1"/>
    <dbReference type="InParanoid" id="W5NCB9"/>
    <dbReference type="OMA" id="MMRCVEL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014842">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01265">
        <property type="entry name" value="PH_TBC1D2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000014">
        <property type="entry name" value="Putative TBC1 domain family member 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000018">
        <property type="entry name" value="TBC domaincontaining protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000018">
        <property type="entry name" value="TBC1 domain family member 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000326">
        <property type="entry name" value="TBC1 domain family member 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="43"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="659"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="484"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="960" mass="110076" checksum="60152E829C9266B4" modified="2014-04-16" version="1">QEKKVQRKMHGGDGSEGSAPRNSRISPAKSLEVSDSDGTKDQGSKLCGYLNKLAGKGPLRGFKTRWFVYDPRKCYLYYFKTPQDALPLGHIEIADACFSYEVEGDEGQFEIHASGKEFVLKAPNRQVMRYWLQQLQQKRWEFCNNRGSASRDSWSSPPLPDLPTGLVARDADAFTFEKPSETTESVRSEFALETSSEDLAGVGVTRNPTFPPTNINFSLKHWGTELRNSMSQLRPGRGSGEGNRRSVFYTGSDDWEMIDPPSRELQEQMAMLEFSKKPHPEAHRNSTGSVFAFDFGRTNARSKRPLLKDVMGGPSKNSRSIETSPVEWNGSKSSETQLRLQGQQEELARLREELASQKELVRLLQQTLKSSQCDRRSAGAPDSPQDSVQLIQEKDRQIQALCGHMEKMALEKESLQQELRTLKNKVGEINDQLGMLMETIQAKDEVIIKLSQQSCPGCESAGYPTRGAPDCSSPNSLCKELQELDKLKDSLQGYKTQNKFLNKEILELSVLRRNAETREKALEAKYTALEAKLCQIESKYLVLLQEVKNPVCSSEQNPAREVISRLLEDALQVESPEHQEHTILKPHPVSEYDVYGFKTVPEDDEEEKLVAKVRALDLKSLSLTDQEVSVGVKWENYLASVMNREMTRTPELKALIRSGIPHEHRCKVWKWCVSFHVKKFRDSVSADYFSLLLQKAYEKPNPASKQIELDLLRTLPNNKHYASPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLAAIALLYLEEEEAFWCLIAIVEVFMPRDYYTKTLLGSQVDQRVFKDLMSEKLPRLHAHFEHYKVDFSLITFNWFLVVFVDSVVSDILFKIWDSFLYEGPKIIFRFALALFKYKEEEILKKQDSMAIFKYLRSFTRAILDARKLMSMAFVDMNPFPLRQIQNRRTFHLEKVRLELTELEAIRQNFLRERETNPDGRNLISDDEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDJ9</accession>
    <name>W5NDJ9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">BRR2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">U5 snRNP-specific 200 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of a core complex containing at least PRPF8, SNRNP200, EFTUD2 and SNRNP40. Component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes, building blocks of the spliceosome. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the minor spliceosome, which splices U12-type introns. Interacts with C9orf78; the interaction is direct and mutually exclusive with its interaction with WBP4. Interacts with WBP4; the interaction is mutually exclusive with its interaction with C9orf78. Interacts with PRPF8. Interacts with TSSC4; the interaction is direct, excludes recruitment of C9ORF78 and WBP4 to SNRNP200 and negatively regulates its RNA helicase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01006172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NDJ9">
        <property type="interactions" value="1859"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018708"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018740.1">
        <property type="protein sequence ID" value="ENSLOCP00000018708.1"/>
        <property type="gene ID" value="ENSLOCG00000015198.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694525"/>
    <dbReference type="KEGG" id="loc:102694525"/>
    <dbReference type="CTD" id="23020"/>
    <dbReference type="eggNOG" id="KOG0951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154966"/>
    <dbReference type="HOGENOM" id="CLU_000335_2_1_1"/>
    <dbReference type="InParanoid" id="W5NDJ9"/>
    <dbReference type="OMA" id="MNPKEFN"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015198">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000048">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000368">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="490"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="684"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="1337"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="1572"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2138" mass="244139" checksum="DE44532BE93CCE23" modified="2014-04-16" version="1">MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLDGTKMGDKSQRTKPQMLEERRAKRRKRDEDRHDINKMKGFTLLSEGIDEMVGIVYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKMRDKERRKEIEQLLGPTDDTRYHVLVNLGKKISDYGGDKELQNMDDNIDETYGVNVQFESDEEEGDEDQYGEVRDEVSDEDSEGVEADVGCTLSANLGSSGDVMTAKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRRMIQYCTMLASAQSEAEKERIISKMEADPDLSKVLYQLQETEKEDIIREERSRRERVRKSRVDNDLEAMDLDQQGETLAPRQLLDLEDLAFTQGSHFMANKRCQLPDGSFRKQRKGYEEVHVPALKPKPFADDEVLVPIEKLPKYAQAGFEGFKTLNRIQSKLFKSAMDTDQNLLVCAPTGAGKTNVALMAMLREIGKHINLDGTINVDEFKIIYVAPMRSLVQEMVGSFGKRLASYGITVAELTGDHQLCKEEINATQIIVCTPEKWDIITRKGGERTYTQLVRLIIIDEIHLLHDDRGPVLESLVARTIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQTAKDAVNWLGYTYLYIRVLRNPTLYGVSHDEKAADPLLERRRLDLVHTAAVVLDKNNLIKYDKRSGSFQVTDLGRIASHFYITHDSIQTYNQLLKPTLSEIELFRVFSLSSEFRNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAYISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTINLCKMIDKRMWQSMSPLRQFRKLPEEVIKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHQFPKLDLSVHLQPITRSTLKVELTITPDFQWDEKVHGTSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLVTFFVPVFEPLPPQYFIRVASDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDRFPFFNPIQTQVFNAVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQNAEGRCVYITPMEALAEQVFADWYEKFQETLSKKVVLLTGETSTDLKLLGKGDIIISTPEKWDILSRRWKQRKNVQNVSLFIIDETHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSTTATFNFHPNVRPVPLELHIQGFNVSHTQTRLLSMAKPVYHAIMKHSPSKPAIVFVPSRKQTRLTAIDILTFCTADITPQRFLHCTEKDLSPYLEKLSDVTLKETLANGVGYLHEGLTPTERRIVEQLFNSGAVQVVVASRSLCWGTSISSHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGRANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCLHDHFNAEIVTKTVENKQDAVDYLTWTFLYRRMTQNPNYYNLQGMSHRHLSDHLSELVEHTLNDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKIRGLIEIISNAAEYTNIPIRHHEDTLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPYFTSEHIKRCTDKGVESIFDIMEMEDDERSGLLQLSDGQMADVARFCNRYPNIELSYEVAEKDGVKSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDPKSNSLISIKRLTLQQKAKVKLDFVAPAQGVHNYTLYFMSDAYMGCDQEYKFSVDVKEADSEGDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDX6</accession>
    <name>W5NDX6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ADP-ribosylation factor-like 13A</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000018835"/>
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        <property type="protein sequence ID" value="ENSLOCP00000018835.1"/>
        <property type="gene ID" value="ENSLOCG00000015307.1"/>
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    <dbReference type="KEGG" id="loc:102688308"/>
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    <dbReference type="eggNOG" id="KOG0073">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161284"/>
    <dbReference type="HOGENOM" id="CLU_040729_3_0_1"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ADP RIBOSYLATION FACTOR LIKE GTPASE 13A"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
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    </feature>
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        <location>
            <begin position="51"/>
            <end position="58"/>
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        <ligand>
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        <ligand>
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        <ligand>
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        </ligand>
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        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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    </feature>
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        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="437" mass="49015" checksum="CD934B14FDC9FEB4" modified="2014-04-16" version="1">MDTDIQLYRLKGRWWPLCCPQPSMFSLASGCCSWLCKLRQPVRKVTVLVLGLDNAGKTSTVRGILKAPLAEVTPTLGCVQLELRVDSFQVTLLDVGGGPEGRGTWRDHYSKAHGIVFVVDSSDAQRLQEARDLLAHLLMHPRVAGKPLLVIANKQDKPKALLGSELVEALSLETLVNQSRSLCHIEPSSALLDARRCSERKTLRGLRWLLRAVCLDYHQLCARVARDCVQPPAPEGPERRGRAERGHGRPREDGIRCSRTPPPKEEPPRSEEKASLPRGKLQPLQNILKKESRLKRRARRKKQVKIKDAVNDGDEEERPEVRTGAEQETEPSPTWHREGKAGSAAAALLPSNRVGLNRSVRVTEDTPEQPDSRQNEKRKPKKGKPEKKKKTKTVKKKNKINSEEVPTAHPQPVDLSTTFDLYRKAILALKARQDQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDY0</accession>
    <name>W5NDY0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000018871.1">
        <property type="protein sequence ID" value="ENSLOCP00000018839.1"/>
        <property type="gene ID" value="ENSLOCG00000015311.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0486">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154518"/>
    <dbReference type="HOGENOM" id="CLU_030301_0_0_1"/>
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    <dbReference type="OMA" id="RRTITTW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015311">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000679">
        <property type="entry name" value="Homeobox protein aristaless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF2">
        <property type="entry name" value="PITUITARY HOMEOBOX 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
        <property type="entry name" value="Homeobox_protein_Pitx/Unc30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="149"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="237" mass="26136" checksum="5D66CFF4DC19FEAD" modified="2014-04-16" version="1">AAAAGSLRVRSGSESSSEEALGPALSGAARLERKHRRQRTHFTSQQLQELEATFQRNRYPDLSIREEIASWTNLTEPRVRVWFKNRRAKWRKKERHMPADMCKPGLGPPLGGLLSPYEDVYSGYPSYGSWGPKALPPKGGYPFFSPVGHLSPSPQPPPSLSAVPLMPDSVPSSLDPLSSPPAVSSPSYCPSQPHFLLRETCLSDPRFRSKQPGSAFSYGPIPSPSPGHCEYRMDRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NE53</accession>
    <name>W5NE53_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Substance-P receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">NK-1 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tachykinin receptor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625420.1">
        <property type="nucleotide sequence ID" value="XM_006625357.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NE53"/>
    <dbReference type="FunCoup" id="W5NE53">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018912"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018944.1">
        <property type="protein sequence ID" value="ENSLOCP00000018912.1"/>
        <property type="gene ID" value="ENSLOCG00000015367.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682349"/>
    <dbReference type="KEGG" id="loc:102682349"/>
    <dbReference type="CTD" id="100003539"/>
    <dbReference type="eggNOG" id="KOG4219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153745"/>
    <dbReference type="HOGENOM" id="CLU_009579_6_1_1"/>
    <dbReference type="InParanoid" id="W5NE53"/>
    <dbReference type="OMA" id="CMIKWPE"/>
    <dbReference type="OrthoDB" id="5981855at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015367">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016496">
        <property type="term" value="F:substance P receptor activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902093">
        <property type="term" value="P:positive regulation of flagellated sperm motility"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Neuromedin-K receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001681">
        <property type="entry name" value="Neurokn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000046">
        <property type="entry name" value="NK1_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925:SF4">
        <property type="entry name" value="SUBSTANCE-P RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01024">
        <property type="entry name" value="NEUROKININ1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00244">
        <property type="entry name" value="NEUROKININR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="51"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="107"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031678"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031714"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601681-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="47388" checksum="327AB80B6B277E44" modified="2014-04-16" version="1">MDPLFATTESQWNDSEQNATDFYSNQFVQPIWTVVLWAIAYTSIVIVSVVGNIVVIWIIVAHKKMRTVTNYFLVNLAFAEASMSAFNTVINFTYAVHNDWYYGLDYCRFHNFFPIAAVFASIYSMTAIALDRYVAIIHPLQQRLSSTETKIIMAVIWFLALCLAFPQYYYSNTTELPGRIVCYVDWPKYVIWNFRRTYYICVAILIYFLPLLVMGCAYLVVGLTLWASEIPGDSSDRYSEQLTAKRKVVKMMIVVVCTFAICWLPFHLYFLLDEFIPHLSEERFIQQVYLTIMWLAMSSTMYNPIIYCCLNERFRAGFKQAFRWCPCVKAADYEGLELKSKRYLHTQNSMYKASRMETTVSTVINAIDEDPEEGLLVKRLSLDLTSNGSSRSISKTVSETSSFYSSTNMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFS7</accession>
    <name>W5NFS7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform</fullName>
            <ecNumber evidence="8">2.7.1.137</ecNumber>
            <ecNumber evidence="6">2.7.1.153</ecNumber>
            <ecNumber evidence="7">2.7.1.154</ecNumber>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Phosphoinositide-3-kinase catalytic gamma polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Serine/threonine protein kinase PIK3CG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">p120-PI3K</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="29">Heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with GRK2 through the PIK helical domain. Interaction with GRK2 is required for targeting to agonist-occupied receptor. Interacts with PDE3B; regulates PDE3B activity and thereby cAMP levels in cells. Interacts with TPM2. Interacts with EPHA8; regulates integrin-mediated cell adhesion to substrate. Interacts with HRAS; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="8"/>
    <dbReference type="EC" id="2.7.1.153" evidence="6"/>
    <dbReference type="EC" id="2.7.1.154" evidence="7"/>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01016247">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFS7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019486"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019518.1">
        <property type="protein sequence ID" value="ENSLOCP00000019486.1"/>
        <property type="gene ID" value="ENSLOCG00000015829.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156858"/>
    <dbReference type="HOGENOM" id="CLU_002191_1_0_1"/>
    <dbReference type="InParanoid" id="W5NFS7"/>
    <dbReference type="OMA" id="WDCDRRF"/>
    <dbReference type="UniPathway" id="UPA00220"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015829">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="C2_PI3K_class_I_gamma"/>
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        <property type="entry name" value="PI3Ka_I"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="PI3/4_kinase"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE"/>
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        <property type="entry name" value="PI3Ka"/>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PIK_HELICAL"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="21" id="KW-0391">Immunity</keyword>
    <keyword evidence="24" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="19" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="36">
        <location>
            <begin position="35"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
            <begin position="218"/>
            <end position="310"/>
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    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="39">
        <location>
            <begin position="358"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="37">
        <location>
            <begin position="543"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="35">
        <location>
            <begin position="799"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00012013"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022657"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023981"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00063154"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00076130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00078766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00080619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00083126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1105" mass="127888" checksum="58EEDFFFFE41299C" modified="2014-04-16" version="1">VMEQREDVELPVVFRDESTRRRRKMKAFTSNSLVTMDQITIEFVLPTSNKFNKTPEVILVDVAGNCTVEQVKTQVWMKAVAANLCPEFYQKYSPDQCILRYQKKGHWYEIYDKHQVFQTLDCIRYWKALKKDQGRIHLASRPQPTDKSQQYQRYLNYLIGYDVTDVSNVHDDELEFTRRKLLTPRMIELAERDPKLYSMDPWITSKPLPEYLLNKISNNHILVVIHRHTSSQTIKVSIDDKPLQILQSFFTKIAKKRSLLGIPEEVSEQDFVLRVCGREEYIFGNHAIKDFHWVRQCLKNAEEIHLVLESAPNPDQDVVHKEDWSLVDDCTGVAGSHEQLTIVEKDHEKVFTISMWDCNRNFRVKVVGIDIPTLPRNSELIVFVEASIFHGQQLLAQDRTSSKPFTEEVLWNTWLEFNIKIKDLPKGARLSLQVFCGKAQTQTGKAGFQESVNYDSKCKNRLLYYVNLLLIDHRSLLRQGEYILHMWKVPEKSEDHSSINADKLTSATNPDKDNSMAIVILLDKYCYPIALPKGQDSVETEGERGRREMPNHLRKQFEEIVVTDPLHPLSPEDKELLWHFKQECMRFPRAYPKFLSSVKWGKQEAVLATHQLLEKHDTWDRSPLDVGLAMQMLDCNFSDANVRTMAVRKLETLGDDDVLRYLLQLVQAVKFEPYHDSALARFLLKRALRSKRIGHFLFWFLRSEIAQSMHYQQRYAVILEAYLRGCGEAMLQDFRKQVEITEALQKVTREIKTMSADKYDVSVQVVFQLRQKLENLQSSGLPGSFRVPYDPGLRAGALVVEQCKVMASKKKPLWLQFKRADPTTLSSDTIGIIFKDGDDLRQDMLILQILLIMESIWETESLDLSLLPYGCISTGNKIGMIEIVKDATTIANIQQSTVGNTGAFKDEILNQWLRERCLNEDKFQLAVERFVYSCAGYCVATYVLGIGDRHNDNIMITESGNLFHIDFGHILGNYKSFLGISKERVPFVLTPDFLYVMGTFGKKTSPHFQKFQDVCVKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEYIREALTVGKTEEEAQKHFLDQIEICRDKGWTVQFNWFLHLVLGIKQGVEKRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NI46</accession>
    <name>W5NI46_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7918.ENSLOCP00000020305"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020339.1">
        <property type="protein sequence ID" value="ENSLOCP00000020305.1"/>
        <property type="gene ID" value="ENSLOCG00000016440.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018717"/>
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        <property type="component" value="Linkage group LG1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 10" id="KW-0288">FMN</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
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        <location>
            <position position="117"/>
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        <ligand>
            <name>FMN</name>
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        </ligand>
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        <location>
            <position position="139"/>
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        </ligand>
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        <ligand>
            <name>glyoxylate</name>
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        <ligand>
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        <ligand>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <person name="MacCallum I."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="154"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
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    <name>W5NIX2_LEPOC</name>
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        <recommendedName>
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        <name type="scientific">Lepisosteus oculatus</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01025779">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025780">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIX2"/>
    <dbReference type="FunCoup" id="W5NIX2">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020581"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020616.1">
        <property type="protein sequence ID" value="ENSLOCP00000020581.1"/>
        <property type="gene ID" value="ENSLOCG00000016661.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159401"/>
    <dbReference type="InParanoid" id="W5NIX2"/>
    <dbReference type="OMA" id="MQHEWIH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016661">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14225">
        <property type="entry name" value="PKc_DYRK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="291"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="579"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="628"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="671"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="715" mass="81945" checksum="2C083FAACCEDBC8B" modified="2014-04-16" version="1">MCSLERESRRSLTGVREESSCPQIKPLCQLQVKEPSEVPGYGKDRIPGSSFCNTALAMMSVPGRRSDRKKAMKAKLEKLIKENDSRFPTLMKSGRQQHTSVQPSRPDAIVHRLQQADTNRLVTNKAYQQKFSSNVGTLPQLEVPIKQMVVTNAKPHHQHESILPHIVNKGLPNGYPAHQEERHKGLQYPTRFSSVESLSDSTSKSHSNKQEKERTLEGQKLPLTPAEALKHFQDRLTEYEQEEIMDYSELWYLGLDSKKIEGSHGTQQNYGYDDEQGSYIKVLHDHIAYRFEVLEVIGKGSFGQVLKCLDHKTKELVAVKIIRNKKRFHHQALVELKILDALRRKDKENSHNVIHMKEYFYFRNHLCISFELLGLNLYELIKKNSFQGFSLALIRRFAYALLKCLQMLHREKIIHCDLKPENILLSQKGQGNIKVVDFGSSCYEQQRVYTYIQSRFYRSPEVILGHPYSMAIDMWSLGCILAELYTGYPLFPGENEVEQIACIMEVLGLPPNEFLQTASRRRLFFDSKGNPRNITNSKGRKRRPNSKDLASVLKTNDPLFLDFLRRCLTWDPQKRMTPDEAMQHEWIQEGRSHKGRPKTRPVRRTAETAPITDNNKEHSYRKMPSNRTAEKGVPDDADKARRQNSGKAEKMPEAERLRPLGASAEEEVTESEPRDAGRMKSAGERPVQLIMKPQPAGQSAEEEPRGPAQTLPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJB2</accession>
    <name>W5NJB2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023635">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023636">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023637">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023638">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJB2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020721"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020757.1">
        <property type="protein sequence ID" value="ENSLOCP00000020721.1"/>
        <property type="gene ID" value="ENSLOCG00000016764.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153561"/>
    <dbReference type="HOGENOM" id="CLU_018169_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJB2"/>
    <dbReference type="OMA" id="FDAPDYI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016764">
        <property type="expression patterns" value="Expressed in camera-type eye and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd17177">
        <property type="entry name" value="FERM_F0_SHANK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000029">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000025">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000048">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000064">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF4">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="338"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="539"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="467"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="615" mass="68280" checksum="AD33177963771F35" modified="2014-04-16" version="1">MPLSPAAGKHEPSDQQHQEEVQQCATNGNQGDSDSSREDTIYDTIRATSEKSPTSPMEEPQGNAIVIRIGIPDLQQTKCLKFNAEAPVWSSKQRILCTLNQSLKDVLNYGLFQPAYNGKAGKFLDEERLLKEYPLPSITPVPYLEFRYKRRVYTQSHLDDKQLAKLHTKANLKKFMEHVQQKNIEKVSKFLERGLDPNFHDPETGECPLTMAAQLEGCAEVIKLLKNGGAHLDFRTRDGITALHKAVRTKNHTALITLLDLGASPDYKDSRGLSPLYHSSMVGGDPYCCELLLHDHAVVGCVDENGWQEIHQACRHGHVQHLEHLLFYGADMSAQNASGNTALHVCALYNQDSCARVLLFRGANKEIKNYNSQTAFQVAIIAGNFDLAEIIKNHKETDVVPFRETPTYTNRRRVTGSGTLSSPRTLLRSASDNNINVEGRPSHSPVPSLRSLPPQLAGQQAPEVADSSLQSTASSRSSHSRSPSLQCVQEEKETENLTLRRHSRGRLSPSTVTPEPSPPPQPPALTGHRGPKRKLYSAVPGRTFIVVKPYTPQGEGEIQLNRGERVKDAQSNEIVLSIGEGGFWEGTVKGRTGWFPAECVEEVQMRQYDPRQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJG4</accession>
    <name>W5NJG4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MOB kinase activator 2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208659.1">
        <property type="nucleotide sequence ID" value="XM_015353173.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJG4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020773"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020809.1">
        <property type="protein sequence ID" value="ENSLOCP00000020773.1"/>
        <property type="gene ID" value="ENSLOCG00000016806.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102699030"/>
    <dbReference type="KEGG" id="loc:102699030"/>
    <dbReference type="eggNOG" id="KOG0440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271909"/>
    <dbReference type="HOGENOM" id="CLU_038321_1_1_1"/>
    <dbReference type="InParanoid" id="W5NJG4"/>
    <dbReference type="OMA" id="EWMAFNT"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016806">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
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    </dbReference>
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        <property type="entry name" value="Mob1_phocein"/>
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        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="234" mass="27148" checksum="ED5783DA1C99EAC8" modified="2014-04-16" version="1">MGGCHSYGKGDVETHDQNLEFSNIPVQKLKNNNTKAAPSLEEKPYLLSVYVSERITDMDMKTLSSLPDGFDKAEWMATHTIAFFKHINLFSSALSEFCTTKTCPTTAAPGNTTYYWQFDHNKKEKCSAPLYIDYAMSYIQELLTDENVFPTSTGSRFPNGFPFLVQKIFLYLFRTLTHLYWAHFREIVKMEMHPHLNTLYIHFMTFSKEFDLLEPMETAIMDDLTEALHRYNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJX1</accession>
    <name>W5NJX1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Collagen alpha-1(XXI) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001797">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJX1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020930"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020966.1">
        <property type="protein sequence ID" value="ENSLOCP00000020930.1"/>
        <property type="gene ID" value="ENSLOCG00000016931.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153769"/>
    <dbReference type="HOGENOM" id="CLU_639275_0_0_1"/>
    <dbReference type="InParanoid" id="W5NJX1"/>
    <dbReference type="OMA" id="WLVNISN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016931">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000068">
        <property type="entry name" value="collagen alpha-1(XXI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000041">
        <property type="entry name" value="Collagen alpha-1(XXI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF90">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869854" description="Collagen alpha-1(XXI) chain" evidence="13">
        <location>
            <begin position="30"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="44"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="457" mass="51168" checksum="D5D44628E32EF2BB" modified="2014-04-16" version="1">MCSMAHKTKLLKLILQSLPVMLLHSFCTAQDEDRRYSCRTAPSDLVFILDGSWSVEDKNFAIVKKWLVNITTNFNIGQRFTQVGVIQYSDDPVLQIPLGTYLSSTELIEAMESIEYMGGNTRTGIAIKFAIDNLFAHSERGPNGVAKIAVVLTDGKSQDEVKLAAEEARKKGIILFAIGVGSETEEEELRAIANKPSSTYVFYVEDYIAITKIREVIRQKLCEETVCPTRIPVAARDEKGFDILIGLDFAKKAKRTKGSYSGSRAYLVTSRLDLTESTRAIFPDGLPPSYVFVATLKYKGSVTTETWDLWRIQALDGTPQMAVTLNGKDKTVMFTTTSMGNDTQTVTFSTQETEKLFDEHWHQIRLLVSEQSVTLYVDGQQTESLELGPAPGIFVNGKTQVGKYFRKEETIPFEIQKLRIYCDPEHNNRETSCEIPGTDGEVRILPGGNTIIEKKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKC9</accession>
    <name>W5NKC9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:11333"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TENT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01026690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626328.1">
        <property type="nucleotide sequence ID" value="XM_006626265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKC9"/>
    <dbReference type="FunCoup" id="W5NKC9">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000021124.1">
        <property type="protein sequence ID" value="ENSLOCP00000021088.1"/>
        <property type="gene ID" value="ENSLOCG00000017065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102682485"/>
    <dbReference type="KEGG" id="loc:102682485"/>
    <dbReference type="CTD" id="100330676"/>
    <dbReference type="eggNOG" id="KOG3852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157191"/>
    <dbReference type="HOGENOM" id="CLU_008115_2_0_1"/>
    <dbReference type="InParanoid" id="W5NKC9"/>
    <dbReference type="OMA" id="CCENPTM"/>
    <dbReference type="OrthoDB" id="10065073at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017065">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012937">
        <property type="entry name" value="TET5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974">
        <property type="entry name" value="PRION-LIKE- Q/N-RICH -DOMAIN-BEARING PROTEIN PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974:SF25">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07984">
        <property type="entry name" value="NTP_transf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01153">
        <property type="entry name" value="DUF1693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="401" mass="45885" checksum="56A93A214EE7457D" modified="2014-04-16" version="1">MADDDNVSASGINFACESTNFNVLSWEQVQRLDIILTETIPIHGRGNFPTLEMKPRQIVKVVRSRLEEKKINVRDVRLNGSAASHVLHEDSGLGYKDLDLIFCADLKGEAEFQTVKDIVLDCLLDFLPEGVNKEKITPLTLKEAYVQKMVKVCNDSDRWSLISLSNNSGKNVELKFVDSLRRQFEFSVDSFQIKLDSLLLFYECSENPMTETFHPTIIGESVYGDFKEALDHLCHKIIATRNPEEIRGGGLLKYCNLLVRGFRAASETEIKSLQRYMCSRFFIDFSDIGEQQRKLESYLQNHFVGLEDRKYDYLMTLHGVVNESTVCLMGHERRQTLNLITMLAIRVLAEQNIIPNVANVTCYYQPAPYVADANFNNYYIAQVQPVFACQQHTYSTWLPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKR5</accession>
    <name>W5NKR5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01029858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NKR5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021224"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021260.1">
        <property type="protein sequence ID" value="ENSLOCP00000021224.1"/>
        <property type="gene ID" value="ENSLOCG00000017171.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016567"/>
    <dbReference type="HOGENOM" id="CLU_009273_7_2_1"/>
    <dbReference type="InParanoid" id="W5NKR5"/>
    <dbReference type="OMA" id="QTVHMTS"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017171">
        <property type="expression patterns" value="Expressed in liver and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000117">
        <property type="entry name" value="molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.640:FF:000002">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_B">
        <property type="entry name" value="MoaC_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047594">
        <property type="entry name" value="MoaC_bact/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="22">
        <location>
            <begin position="59"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="647" mass="70945" checksum="C4126E8C5D256365" modified="2014-04-16" version="1">MQSSYSGATPEEKVEPLSPDPTVSVAAKTASVQELAPPGGRSRELSEDPQPFSAFLTDRFGRQHRYLRISLTEKCNLRCQYCMPEEGVKLTPRSQLLSTAEVLALARLFVQEGVEKIRLTGGEPLIRPDVVHVVAELRKLQGLKTIAVTTNGMNLARLLPSLKEAGLDLLNISLDSLVPAKFEFIVRRKGFHKVMEGIDKAIELGYNPVKVNCVVMRGLNEDELLDFVALTEKKPLEVRFIEYMPFDGNKWNFRKMVSYQEMLDRIRQRWPDLEKLPAGETDTAKIFKVPGFQGQVGFITSMSEHFCGSCNRLRVTADGNLKVCLFGNAEVSLRDCLRSGASEEELLQIIGAAVGRKKQQHAGMFSISQMKNRPMILIVSEAPKGRCQHLPNSTRPQLFNCICTPVSTKYPQHCSPHRSLSLLQTIWLRQTLGALTAIVSALSNSYTVRIEVRSAPSLLQALAKGERKRIAMPKMDSRGSVAALKGVHGPSDHLTHTDAQGRASMVDVGGKPVSRRTAAASATVLLGEKAFWLVKENQLAKGDALAVAQIAGIMAAKQTSALIPLCHPIPLDRVAVSLELVEPGWRVVVTATCVASGRTGVEMEALTAASLAALALYDMCKAVTRDIVIQDVRLLSKTGGQRGDYHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLI8</accession>
    <name>W5NLI8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 55a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLI8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021497"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021534.1">
        <property type="protein sequence ID" value="ENSLOCP00000021497.1"/>
        <property type="gene ID" value="ENSLOCG00000017392.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QV79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
    <dbReference type="InParanoid" id="W5NLI8"/>
    <dbReference type="OMA" id="LDGVCYY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017392">
        <property type="expression patterns" value="Expressed in bone element and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004947">
        <property type="term" value="F:bradykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15165">
        <property type="entry name" value="7tmA_GPR55-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000142">
        <property type="entry name" value="G protein-coupled receptor 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000496">
        <property type="entry name" value="Brdyknn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF51">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00425">
        <property type="entry name" value="BRADYKININR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="32"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="36026" checksum="7EAC618285A35A0C" modified="2014-04-16" version="1">FQHRMSGNEECSLVCWIQWIVYIPTFVVGLVFNLSALWILLFKIRKCTESTVYLANLILNDSFLLFSLPFKIHSYKYNWVLGNTLCSFLESLLYVNMYGSILLITCISVDRYIALRHPFLAKTLRSPQKAAVACFTIWIVIFGASVEVYNLHEKNATYCFRGFSNRTWEKVHIVVVMETVYTASALIMIFCSIQVIRTLRTMRKRKAEVQTLKNNKSVKIVLSNLVTFLVCFTPYHIAALVYFIAKKKVTESIQPLRDFVHLSICLTSINCLFDAICYYFIFKENWISAKQANEQEQQCMKKSENIPTVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLK3</accession>
    <name>W5NLK3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sulfotransferase</fullName>
            <ecNumber evidence="20">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-{N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67860"/>
            <dbReference type="Rhea" id="RHEA-COMP:17365"/>
            <dbReference type="Rhea" id="RHEA-COMP:17366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:176490"/>
            <dbReference type="ChEBI" id="CHEBI:176491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3-O-{N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67856"/>
            <dbReference type="Rhea" id="RHEA-COMP:17368"/>
            <dbReference type="Rhea" id="RHEA-COMP:17369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:176489"/>
            <dbReference type="ChEBI" id="CHEBI:176492"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1-&gt;3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67868"/>
            <dbReference type="Rhea" id="RHEA-COMP:14419"/>
            <dbReference type="Rhea" id="RHEA-COMP:17367"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:139605"/>
            <dbReference type="ChEBI" id="CHEBI:176494"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:67869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1-&gt;3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67864"/>
            <dbReference type="Rhea" id="RHEA-COMP:14420"/>
            <dbReference type="Rhea" id="RHEA-COMP:17370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:139607"/>
            <dbReference type="ChEBI" id="CHEBI:176493"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:67865"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18">Protein modification; carbohydrate sulfation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="20"/>
    <dbReference type="EMBL" id="AHAT01015287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015216723.1">
        <property type="nucleotide sequence ID" value="XM_015361237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLK3"/>
    <dbReference type="FunCoup" id="W5NLK3">
        <property type="interactions" value="631"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021512"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021549.1">
        <property type="protein sequence ID" value="ENSLOCP00000021512.1"/>
        <property type="gene ID" value="ENSLOCG00000017407.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692772"/>
    <dbReference type="KEGG" id="loc:102692772"/>
    <dbReference type="CTD" id="100006249"/>
    <dbReference type="eggNOG" id="ENOG502QTSD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161292"/>
    <dbReference type="HOGENOM" id="CLU_028381_1_0_1"/>
    <dbReference type="InParanoid" id="W5NLK3"/>
    <dbReference type="OMA" id="DYKWHKG"/>
    <dbReference type="OrthoDB" id="6138663at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017407">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000765">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016469">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF3">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005883">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="22">
        <location>
            <begin position="261"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="463" mass="53367" checksum="F85CCBF2A42C35D1" modified="2014-04-16" version="1">MKSKHYQQFKFAPWEKDASFGRKLKTYRNRTKIIAHPGLVMKVLRRKRIVLFIAYFLLLILTMLNLANYKWTKEPQQCNHQMRSTPYQSRSDIRFLYRPSVVKKRQLVYVLTTWRSGSSFFGELFNQNPEVFFLYEPMWHIWQKLYPGDAVSLQGAARDMLSSLYRCDLSVFQLYNTPGGKNVTSLGLFGATLNKVICSYPLCSAFRKDVVGMVDDKVCKKCPPQSLRILEDECLKYSTVVIKGVRILDVNVLAPLMEDPSLDLKVIHLVRDPRAVANSRIKSRHGLIRENLQVVRSRDPKLRRIPFVDPNHKVNKKEGSDYHSIGAMEVICDRTSKTLRTALNPPHWLKGKYMTVRYEDLVEDPVKTVRNVYRFVNLSANHDIESFALNMTSGTSSSSKPFIVSSRNATQAASAWRTVLSFQQIKQVEDYCHHSMAMLGYERVRTAGEVKDLSKSLLTVPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMP2</accession>
    <name>W5NMP2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein ABHD13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alpha/beta hydrolase domain-containing protein 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine esterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219879.1">
        <property type="nucleotide sequence ID" value="XM_015364393.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015219880.1">
        <property type="nucleotide sequence ID" value="XM_015364394.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMP2"/>
    <dbReference type="FunCoup" id="W5NMP2">
        <property type="interactions" value="1057"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021901"/>
    <dbReference type="ESTHER" id="lepoc-w5nmp2">
        <property type="family name" value="ABHD13-BEM46"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.051"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021939.1">
        <property type="protein sequence ID" value="ENSLOCP00000021901.1"/>
        <property type="gene ID" value="ENSLOCG00000017797.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698041"/>
    <dbReference type="KEGG" id="loc:102698041"/>
    <dbReference type="CTD" id="84945"/>
    <dbReference type="eggNOG" id="KOG4391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158114"/>
    <dbReference type="HOGENOM" id="CLU_029375_2_0_1"/>
    <dbReference type="InParanoid" id="W5NMP2"/>
    <dbReference type="OMA" id="QYWTSED"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017797">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000058">
        <property type="entry name" value="Alpha/beta hydrolase domain-containing protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="12">
        <location>
            <begin position="115"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="337" mass="38217" checksum="58DF2CCFFE68AE51" modified="2014-04-16" version="1">MEKSWRLWTIAEKWLVNLASWSWGLCRISLLALILTFHLYGGFMLLALIFASVAGILYKFQDVLLYFPDQPSSSRLYVPVPTGIPHENIFIKTKDGMCLNLIFLRYTGDNPAFVPTIIYFHGNAGNIGHRIPNALLMLVNLKVNVVLVDYRGYGKSDGEPSEDGLYLDSEAVLDYVMSRPDIDKTKIILFGRSLGGAVAIHLASGNPHRISAIMVENTFLSIPHMASTLFSFFPMRYLPLWCYKNKFLSYRKIAQCRMPSLFISGLSDQLIPPVMMKQLYELSPSRTKRLAIFPEGTHNDTWQCQGYFTALEQFIKDLLKNHTQEETVKSASSVTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNJ7</accession>
    <name>W5NNJ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Suppressor of cytokine signaling 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity. Regulates IFN-gamma (IFNG)-mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the SOCS1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637341.1">
        <property type="nucleotide sequence ID" value="XM_006637278.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015215575.1">
        <property type="nucleotide sequence ID" value="XM_015360089.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015215576.1">
        <property type="nucleotide sequence ID" value="XM_015360090.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNJ7"/>
    <dbReference type="SMR" id="W5NNJ7"/>
    <dbReference type="FunCoup" id="W5NNJ7">
        <property type="interactions" value="492"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022206"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022247.1">
        <property type="protein sequence ID" value="ENSLOCP00000022206.1"/>
        <property type="gene ID" value="ENSLOCG00000018105.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693371"/>
    <dbReference type="KEGG" id="loc:102693371"/>
    <dbReference type="CTD" id="445073"/>
    <dbReference type="eggNOG" id="KOG4566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161164"/>
    <dbReference type="HOGENOM" id="CLU_079452_2_1_1"/>
    <dbReference type="InParanoid" id="W5NNJ7"/>
    <dbReference type="OMA" id="YCSITRA"/>
    <dbReference type="OrthoDB" id="9937362at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018105">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046426">
        <property type="term" value="P:negative regulation of receptor signaling pathway via JAK-STAT"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0060336">
        <property type="term" value="P:negative regulation of type II interferon-mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000406">
        <property type="term" value="P:positive regulation of T cell migration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032868">
        <property type="term" value="P:response to insulin"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10382">
        <property type="entry name" value="SH2_SOCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Suppressor of cytokine signaling 1"/>
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    </dbReference>
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        <property type="entry name" value="Suppressor of cytokine signaling 1"/>
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    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035861">
        <property type="entry name" value="SOCS1_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF4">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="70"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="16">
        <location>
            <begin position="152"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="202" mass="23393" checksum="0735E5674464D344" modified="2014-04-16" version="1">MVAHSNVEDNASEQNQARAELPAPASPRRHGEPRQRVARPESPTRFRHFRSERDFRTITMTTSMLENGGFYWGPMTVEEAHTKLKTEPQGTFLIRDSRQKDYFFTLSVKCASGPTSIRIDFQNSRFSLVGSKESFTCLFRLLEHYMDSPKKSLVKPYRKVKVQSLQELCRKRIIETFGKDNIDSIPVNAVLKDFLKSFPFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNT8</accession>
    <name>W5NNT8_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Si:ch211-210c8.7</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018711">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNT8"/>
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        <property type="gene ID" value="ENSLOCG00000018196.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000087489"/>
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    <dbReference type="InParanoid" id="W5NNT8"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018196">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="60" mass="6559" checksum="89BD4F0E1CA9E714" modified="2014-04-16" version="1">VSPVVIVLVLLTLAGAAYLLFRYQCQNKGAYRTTGEPAPGEELEQVAAEASPEDKKEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNZ4</accession>
    <name>W5NNZ4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNZ4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022353"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022394.1">
        <property type="protein sequence ID" value="ENSLOCP00000022353.1"/>
        <property type="gene ID" value="ENSLOCG00000018252.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="HOGENOM" id="CLU_000384_6_0_1"/>
    <dbReference type="InParanoid" id="W5NNZ4"/>
    <dbReference type="OMA" id="FLECGCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018252">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="390" mass="43404" checksum="D6848FBA7CD05179" modified="2014-04-16" version="1">MERVEVDVLGPFPHTEAGNRYVLVAMDYFTQWPEAYALLDQSAPTVADALLEGMFARLGVPEELHSDQGRNFESQVFARVCQRLGITKTRTTPLHPQSDGLVERFNQTLATQLATLVSRHQRDWDRHLPLALWAYRTTVQESTGCTPASLLLGREMRTPVDLVFGPPLGDGPAGPDYEWDLRHWMQRVHSFARTHLTQAGVRQKRYYDLRCRGPAFQPGDLVWVYNPCRRKGLSPKLAPSWEGPTEVLRVVGEVCYRVRLRTRGRVVVLHRDRLAPYRAREEEPCGEALEEQPGLEPADSGASVDRQPSPRHSGRSRRVPQQLAEYEYSLRGVFDVGETPDASGGGSVTPSPGGEEEAPLGARSTAGHAGSGSQGESEGQHDDQRLTLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWI3</accession>
    <name>W5LWI3_LEPOC</name>
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        <submittedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 99-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWI3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000490"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000491.1">
        <property type="protein sequence ID" value="ENSLOCP00000000490.1"/>
        <property type="gene ID" value="ENSLOCG00000000449.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102685908"/>
    <dbReference type="eggNOG" id="ENOG502QTX0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000243802"/>
    <dbReference type="HOGENOM" id="CLU_910685_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWI3"/>
    <dbReference type="OMA" id="NILAHIT"/>
    <dbReference type="OrthoDB" id="10262255at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000449">
        <property type="expression patterns" value="Expressed in testis and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="292" mass="34183" checksum="C442EAF71F8E3995" modified="2014-04-16" version="1">MRELDLQRQLADVERRRLEGQLSHEEAVLARHRLLQENQRKAQLKKEETAEMMRRYAELRLEEEKGMKELVEVVAEGHKNSKEAKAKLKEYKQRIVQEVSEQSRELLRQALEEAQAELSRKFELIRQIRALESVPVIRHRFVDLTEAGGHALLGEMSVVELRERLALLKEAARREQEERRDRILEEKQVREQLLLEQLDHIALHRAVLGRAAALRQEEKKARAPALRARAIQDERVSELQRKLEEKRQQRQRQSESLKMKAEKSTARSLKAWSVKKVTHTPGPGARTLQPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWL6</accession>
    <name>W5LWL6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWL6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000523"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000523.1">
        <property type="protein sequence ID" value="ENSLOCP00000000523.1"/>
        <property type="gene ID" value="ENSLOCG00000000477.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064112"/>
    <dbReference type="HOGENOM" id="CLU_1820217_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWL6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000477">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026254">
        <property type="entry name" value="RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004">
        <property type="entry name" value="RING FINGER PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15963" checksum="4CD1572B3B7CD549" modified="2014-04-16" version="1">MQALISCQYSVCLNPSLSPGVSDTGSDLLVQCVYEPFSLLVSQIQALICQYSVCMNPSLSLLVFQMQALISCQCTVCSACFQQHFTIAVRDKHIRDMVCPVCGEPDINNQDQFNSYFSTLDIQLRDCLDSEVYELFHKKLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXW1</accession>
    <name>W5LXW1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LXW1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000968"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000972.1">
        <property type="protein sequence ID" value="ENSLOCP00000000968.1"/>
        <property type="gene ID" value="ENSLOCG00000000870.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LXW1"/>
    <dbReference type="OMA" id="GQHYLNS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000870">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002315">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="47"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="179" mass="19679" checksum="03DEF8E02B4211E7" modified="2014-04-16" version="1">TYTLMRDSMAYMIIEKLPFMATESTATMAFITIFIWTLLITVQESNGQISVTQTPRISIAAGQTLTVNCKTSPAVYNNNRLSWYQQKAGEAPNLLIYDATTRQTGIPERFSGSGSGTDFTLTITGVQAEDAADYYCQGQHYLNSKWVFTQCYTLVQKPLSAGLHSNCTAAAGTYCRCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYV9</accession>
    <name>W5LYV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Differentially expressed in FDCP 8 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts. Involved in bone resorption.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010680">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYV9"/>
    <dbReference type="FunCoup" id="W5LYV9">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001316"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001321.1">
        <property type="protein sequence ID" value="ENSLOCP00000001316.1"/>
        <property type="gene ID" value="ENSLOCG00000001166.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1829">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159182"/>
    <dbReference type="InParanoid" id="W5LYV9"/>
    <dbReference type="OMA" id="NMICPKC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001166">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20819">
        <property type="entry name" value="C1_DEF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000042">
        <property type="entry name" value="differentially expressed in FDCP 8 homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051366">
        <property type="entry name" value="DEF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047983">
        <property type="entry name" value="DEF8_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326:SF3">
        <property type="entry name" value="DIFFERENTIALLY EXPRESSED IN FDCP 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01175">
        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="7">
        <location>
            <begin position="148"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="53083" checksum="561417A58A408332" modified="2014-04-16" version="1">VAGAMEYDERLARFRQGLVNPFDRGEGPPEEGPLQHDIGRPLTMMFCEVYAEVKPELFSSEVPVHSSDRTMDLGLAEDHFSRPSGSFVASDVEQLKQAIEECKQLILELPEHSERQKDTVVKLIHLRLKLQELKDPDEEEPNLRVLLEHRFSKEKSKSVKQTCDKCSAIIWGLIQTWYTCTGCYYRCHSKCLNLITKPCVRSKVSHQSEYELNICPEVGLDSQDYRCAECRAPVSLRGVPSEARQCDYTGQYYCSSCHWNDAAVVPARVIHNWEFEPRKVCRSSMRYLNLMISRPVLKLREINPLLFNFVEELVEIRKLRQDILLMKPYFITCKEAMEARLLLQLQDRQHFVENNDMYSLQDLIDISTGRLSCSLTEIHTTFAKHIKLDCERCQAKGFVCELCKEGDVLFPFDSHTSVCHDCSAVFHRDCYYDNSTACPRCTRMSERKQSIEPPTPRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYW0</accession>
    <name>W5LYW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021855">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021860">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001317"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001322.1">
        <property type="protein sequence ID" value="ENSLOCP00000001317.1"/>
        <property type="gene ID" value="ENSLOCG00000001169.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164732"/>
    <dbReference type="HOGENOM" id="CLU_008233_0_0_1"/>
    <dbReference type="InParanoid" id="W5LYW0"/>
    <dbReference type="OMA" id="DLCGWYQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001169">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="961"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="5">
        <location>
            <begin position="193"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="5">
        <location>
            <begin position="555"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="5">
        <location>
            <begin position="630"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="5">
        <location>
            <begin position="724"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="5">
        <location>
            <begin position="772"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="992"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="381"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1019" mass="112036" checksum="8117F90CB3531A55" modified="2014-04-16" version="1">CDFRCDSADCCDELDCGEQARYGKEFECDFEVSSCGWKDKXWFMAVAGLSGVSDVALLVSPNMQQAAATCEVRLRYFVWDSDRRWCFSPQKKNNSWVHREDFRTKRFQIAGLDIIPRVALEFDFRKASGDESGPVLFSGLSPKKCLLPKPDSCPGGSTACRRGGCVRAEQVCDGTDDCGDGSDESEGLCGHYRKCDFESGTCEWSQGEESNLSWSRINQTQTYVTFDPQAGPGRDHTLNMAAGHFLYISSPMSDGAHWTHLHSPVLEPTNSTHPCTLVLYAHLFGPRVGVLSVLILTPKSLKPVWHKDGNHGDLWFKGQVDIVVNSTFQVLRVRGCVKANCKGVVKKPPPLLPKIPPHHASCEVGQFECAKGGCVQQDRVCDFALDCPDGSDEAECGFIGNTSFETSSRGWTDLSVGTLEWTVTTLDTDSSQDICVCPVYSWIYLWIFPGIKLHVRVCVLRDMHRCIKVDRCDYMYVSKCRQVGRCVYINGCPGGGGESDQLLNCTVCKYGRHSLHYPIGQALYPRFSINGAVTDTVKTTLAGMYSTAVSVDMPPAGSLSVKLRWHSDGAETVLWTRRGAHGNTWHLGNCPIAPSSGNFQLVLEALRDGYDGRVAVDDVMVLSQPCPAHTSCSFDASACEFSSTGAERWVRQNRVSGSISHGPETDHSLGTETGTTLVFFGDVTYVAGGPNQSQLLALCFFTYSVSLSPDKIRLELGSPVQLFWSRSSSEITGAKESERKLVFEAEGAGGVGTHIAIDDITFSPHSCPEPASVCDFERGMCSWTNTQNPKLDQLDWDWTSTEVERQYPTPPHDHSLGTVHGHFLFLPSSPRETAGQKAWLLSPHLPPTRGTCLRFWAYRNPLCKNHTCPPYSVYVAAEGRLRVVLLSAGEAVELFRVEGAEQWTRYDVLIRSEREYQIVFEGVKGHSGVQALDDIIYTPGVNCPGEQPDPPSSAEPPDSTAGVVASIIILLLLLATLAVLLVFYLRHRQGAAPSHTPEMSFNNITYSSSDQVQGTGPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZL7</accession>
    <name>W5LZL7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTPase-activating protein and VPS9 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GAPVD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021808">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021809">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021810">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01021813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001574"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001579.1">
        <property type="protein sequence ID" value="ENSLOCP00000001574.1"/>
        <property type="gene ID" value="ENSLOCG00000001381.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156611"/>
    <dbReference type="HOGENOM" id="CLU_002165_1_0_1"/>
    <dbReference type="InParanoid" id="W5LZL7"/>
    <dbReference type="OMA" id="ENHEIML"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001381">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05129">
        <property type="entry name" value="RasGAP_RAP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000009">
        <property type="entry name" value="GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.80:FF:000001">
        <property type="entry name" value="GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="12">
        <location>
            <begin position="144"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="13">
        <location>
            <begin position="1348"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="601"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="765"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="879"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="916"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="884"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="919"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="984"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1027"/>
            <end position="1036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1488" mass="165233" checksum="D4A0D44A3809786D" modified="2014-04-16" version="1">MVKPDIHTLAHHLKQERLYVNSEKQLIQRLNAEVLKTAEKLYRAAWIAKQQRINLDRFILTSAEASPAECCQHAKILEDTQFVDGYKTLGFQESIYGEFLSRLRENPRLVASCLVTGEKLNQEHTQGVTHTVFTSLYGNCIMQEDEHYLLQVLRYLIEFELKESDNPRRLLRRGTCVFSTLFKLFSEGLYSAKLFLTATLHEPIMQLLVEDEDHLETDPSKVTERFTPAQQERMFGDKGSEGYRRKVQEMVESNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVERLDLGEVRTMCTDLLLTCFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMAGTDEGDPRVKSSLSKFDKSCVAAFLDVVIGGRAVETPPMSSVNLLEGLTRTVVYIAQGQLLALVDFVRNVMSGDQLREEERLALETLLANVPQSRPGKSNSLELTPSNTPQLSPATTPANKKNRLPIGQHLAAITCWDPSSANLSAHIPLVTPFAASRSRSRSNIAQDQQGELEACSQDSLQEVMPEEVLVISLGTAPQTVPGMMSENEVLTLQMADGGQGEVPVDDTKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGATVENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGVGSTSDDTEVREVGSRPSTPGLSAVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDGEESGHGAHHLTSPPSQVDSLLAMFDPLAMGDGFLGSATSTVVRPKVHYARPPHPPPDPPIPEAFATGQEPRLSMFVPHGLSQAELEQGKQRHSYPDRLVRSRSSDIMCAARRPTSDPGLNRRPNLEERDLPGGVPAQGVFTMGHSSSPSKDSLKGEAEDRKDSDDEKSDRNKPWWKKRFVPAIPKGPMAFRKKDKQEKEDQGPDRVPLTEEPVSRLTPQVQAAEDILDKYRNIKRMGPSEGVVSASYEGPEPCGEGESVHGPAREEALQNISADDLPDSASQAAQPQDSKFSFSDAKKKLRMALCSADSVVFPILTHPTTRNGLPDHTDPEENEIVCFLKVQLAEAINLQDKNQMAQIQETTRCVSRFDSRTCKKLLAAIAEDYRKRAPYIAYLTRCRQGLQTSQAHLERLLQRVLRDKEVATRYFTTVCVRLLLERMESRMLNFINAFQACTAADDKTAAVEDFLRDLYGAMAHDAIWQYASEEQLQDAQMAIERSVMNRIFKLAFYPNQDGDTLRDQVLQEHIQRLSKVVTANHKALQIPEVYLQEAPWPSAQAEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTIQYISNFYASRLSGEECYWWMQFTAAVEFIKTIDDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5M032</accession>
    <name>W5M032_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein O-GlcNAcase</fullName>
            <ecNumber evidence="6">3.2.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = N-acetyl-D-glucosamine + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:48876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:48892"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.169" evidence="6"/>
    <dbReference type="EMBL" id="AHAT01024516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024520">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M032"/>
    <dbReference type="FunCoup" id="W5M032">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001739"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001744.1">
        <property type="protein sequence ID" value="ENSLOCP00000001739.1"/>
        <property type="gene ID" value="ENSLOCG00000001521.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007726"/>
    <dbReference type="HOGENOM" id="CLU_009837_0_0_1"/>
    <dbReference type="InParanoid" id="W5M032"/>
    <dbReference type="OMA" id="CPTYCGS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001521">
        <property type="expression patterns" value="Expressed in brain and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102571">
        <property type="term" value="F:[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015929">
        <property type="term" value="F:hexosaminidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000009">
        <property type="entry name" value="O-GlcNAcase BT_4395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.240:FF:000003">
        <property type="entry name" value="Si:dkey-183c6.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.240">
        <property type="entry name" value="STAT, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051822">
        <property type="entry name" value="Glycosyl_Hydrolase_84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011496">
        <property type="entry name" value="O-GlcNAcase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170">
        <property type="entry name" value="O-GLCNACASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170:SF23">
        <property type="entry name" value="PROTEIN O-GLCNACASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07555">
        <property type="entry name" value="NAGidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52009">
        <property type="entry name" value="GH84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH84" evidence="9">
        <location>
            <begin position="10"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="878" mass="97775" checksum="9BA7138EBADBD6D8" modified="2014-04-16" version="1">MISPCWLQYTVCVCLAGFYGRPWSMEQRKVLFQWMQRWGLNTYLYGPKDDLKHRLLWREVYSPEEAAQLRPLVEEAASRDLQFVYALSPGQDIVFSSPCDVGLLKRKLKQVVADFGCKAFAILFDDIDHSLCQADKEAFSSFAHAQVSVANEIFRFLGEPPIFLFCPTEYCNSLCSPSLAKSPYLNTVGEDLLPGITVIWTGNKVISRELSVASVMEVQAVLQRPPLVWDNLHANDYDSRRVFLGPFKGRPPGLRAHLRGLLLNPNCEFEANYISLHTLGTWHRLSAKPGQVQEHVFSDSCKDSMFVDIFNTVQSSDFGGEVQQVTYCPEEALLLALKDWMQEMSKPLQPACSVPLEGAGGGGSLVCTRPSHSSAEGMTTTGLFDILFLTPAGVLLTKDSRPTVVPAWGSAQHEEPEAESGSSSPLTGVRRLSAPSAEVKIPKDKGQGEQELVVAPRSEDRGLRLNVPHGEMEGEEGEREVQDTPAGKELPLVRTQSQEKGKGGGAAVGRAPLSEEEVKLLVGLYYLPHEHGPPAQRLLQDLAWLKAHSHCVSANGKKGEPQKVEEWRLRAGRFQQDCDRIARLHGSVVNCTNRAVLYDLYPYVWDLRNTVLVTKAFVSWLGCRTNASVDLLGVESEPWVFKGGLSGEFQMLLPTGTNNDLFSHPPPLFPSSRLYNIRPYQTKDKVELYRICRQLHLKTQSSPDGHQPHPDLMGDRWLGACFALCPEYSFVLEDERGLCGCALGALDIRAFLKRSEATWLPTMREKHPRGPGAASHPAAQEALQYFHSEHPDYPDSLLYHFPSVLRLEALPEVLDTSVTRSLVTSLLSALKANGSQGVFCEVLPTDRSRLEFFTKLGFLEIFRCEAVTREEVVLGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3H4</accession>
    <name>W5M3H4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SEC22 homolog A, vesicle trafficking protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in vesicle transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3H4"/>
    <dbReference type="FunCoup" id="W5M3H4">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002932"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002938.1">
        <property type="protein sequence ID" value="ENSLOCP00000002932.1"/>
        <property type="gene ID" value="ENSLOCG00000002494.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0862">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158470"/>
    <dbReference type="HOGENOM" id="CLU_054453_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3H4"/>
    <dbReference type="OMA" id="NTGMQEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002494">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059071">
        <property type="entry name" value="SEC22a-c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043546">
        <property type="entry name" value="Sec22a/c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258:SF3">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25970">
        <property type="entry name" value="SEC22a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="12">
        <location>
            <begin position="8"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="304" mass="34560" checksum="AC94D38438ADD450" modified="2014-04-16" version="1">MSMVLFASVVRVRDGLPLSASTDYEQDKGVQESKKYLKSLSKKLSQFPDRCTLKTGQYNINFTSSLGVGYMMVCTENYPNVLAFCFLDELQREFIVSYDTKRINSAVRPYSFIEFEDNFIQKTKQRYNSPRSLSTKMNLADMQTEIKLRPPYQLSAADVGTVNGFSHPSSSIYKGIAPNQMLEPVTLAGIVSCLLSLLCGGLNLIRGVHAIESILQNEDEDFNYVVAFFLGSTACLYQCYLFAYCTVWRNIKSFLAFALICLCNMYLYELRNMWQILFHVTVGAFTTLQIRLRQPQGKAPDYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3Q5</accession>
    <name>W5M3Q5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Catenin alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Alpha N-catenin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020503">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3Q5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003013"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003019.1">
        <property type="protein sequence ID" value="ENSLOCP00000003013.1"/>
        <property type="gene ID" value="ENSLOCG00000002566.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234543"/>
    <dbReference type="HOGENOM" id="CLU_015314_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3Q5"/>
    <dbReference type="OMA" id="CTISDRI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002566">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000007">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000008">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000011">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.810">
        <property type="entry name" value="Vinculin, Vh2 four-helix bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF23">
        <property type="entry name" value="CATENIN ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="521" mass="57944" checksum="E584655F27B55857" modified="2014-04-16" version="1">MDHISDSFLETNVPLLVLIEAAKSGNEKEVKEYAQVFREHANKLVEVANLACSISNNEEGVKLVRMAATQIDSLCPQVINAALTLAARPQSKVAQDNMDVFKDQWEKQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVIALQEGDVDTLDRTAGAIRGRAARVVHIINAEMENYEPGVYTERVLEAIKLLSETVMPRFAEQVEVAIEALSTNPPQPFEENEFIDASRLVYDGVRDIRKAVLMIRTPEELEDDSDFEQEDYDARSRTSVQTEDDQLIAGQSARAIMAQLPQEEKAKIAEQVESFRQEKCKLDAEVAKWDDNGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNSSDVINAAKKIAEAGSRMDKLARAVADQCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELIVSGLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYGTAAVNSPVVSWRMKAPEKKPLVKREKPEECQTRVRRGSQKKHISPVQALSEFKAMDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M445</accession>
    <name>W5M445_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rh blood group, D antigen</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as an ammonia transporter. May play a role in the elimination of ammonia in the gill.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631426.1">
        <property type="nucleotide sequence ID" value="XM_006631363.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M445"/>
    <dbReference type="FunCoup" id="W5M445">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003153"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003160.1">
        <property type="protein sequence ID" value="ENSLOCP00000003153.1"/>
        <property type="gene ID" value="ENSLOCG00000002671.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691137"/>
    <dbReference type="KEGG" id="loc:102691137"/>
    <dbReference type="CTD" id="6007"/>
    <dbReference type="eggNOG" id="KOG3796">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182844"/>
    <dbReference type="HOGENOM" id="CLU_021386_1_0_1"/>
    <dbReference type="InParanoid" id="W5M445"/>
    <dbReference type="OMA" id="IHVFATY"/>
    <dbReference type="OrthoDB" id="8884035at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002671">
        <property type="expression patterns" value="Expressed in embryo and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000009">
        <property type="entry name" value="Blood group Rh(D) polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF120">
        <property type="entry name" value="RH BLOOD GROUP, D ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="8">
        <location>
            <begin position="43"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="413" mass="45712" checksum="B920C1EA5AE6E86F" modified="2014-04-16" version="1">MAPKYFTSLRPHLPLLVLLLETAFILVFFFFASYDAKNPPDLHLYPEFQDVHVMVFLGFGFLGTFLARYGFSSSGFNVLVAAMTAQWAVLMNGFLFAFGNRTIVVNLRSLLTANLCAVSAFVSMGVVLGKTSPIQLLLMALLEVSGFILNQWILLRFLEVDLTESIRQLHVFGTYFGVMFSWATYRPGLEPRHEKEKSDTKMDLFSMIGTLFLWMFWPSFNSVLIEDTAQKISAVYSTYFSLAASTVAAFGLSVLTSKKGKINMVHVHNATVAGGVAVGTAVTVIQAPWVAMTIGVIAGLTSTLGLQYIKPRMASLFQVHDTCGVQYVHGIPGILGVFANITIQLATNWTSFILTAKEAVFQLTALSVTAALSLILGLGTGYLLKWNFWKAPHDKKCFDDQAYWKFPHLAMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M518</accession>
    <name>W5M518_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G protein-coupled receptor 139</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01037165">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637109.1">
        <property type="nucleotide sequence ID" value="XM_006637046.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M518"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003476"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003483.1">
        <property type="protein sequence ID" value="ENSLOCP00000003476.1"/>
        <property type="gene ID" value="ENSLOCG00000002954.1"/>
    </dbReference>
    <dbReference type="GeneID" id="138216017"/>
    <dbReference type="KEGG" id="loc:102697705"/>
    <dbReference type="CTD" id="124274"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159473"/>
    <dbReference type="HOGENOM" id="CLU_009579_24_1_1"/>
    <dbReference type="InParanoid" id="W5M518"/>
    <dbReference type="OMA" id="WLVHIVS"/>
    <dbReference type="OrthoDB" id="5864054at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002954">
        <property type="expression patterns" value="Expressed in ovary and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000126">
        <property type="entry name" value="Probable G-protein coupled receptor 139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052477">
        <property type="entry name" value="Orphan_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 139-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="40"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="351" mass="40030" checksum="B14819AAE77CFE94" modified="2014-04-16" version="1">MEHNHIFNNSTGQPHSLQSCPLGLLPVIYYSALLCLGLPANILTVIILSQLVSRRQKSSYNYLLALAAADILVLFLIVFVDFLLEDFILSQPLPPSLNKAVQVLEFSSIHTSIWITVPLTVDRYIAVCHPLKYHTVSYPARTRKVIVTVYLTCLLTSAPYYWWPDLWQVDYSSSMAHQVLVWAHCVTVYLVPCSIFFVLNSIIVHKLRRRRSCFRLRGYSTGKTTAILLTITTIFAILWAPRVVMILYHLYVSPIASARVVHLVTDVANMLALLNTGINFFLYCFISKRFRGMAAAMLRAFFRCQKQPVQFYTNHNFSITSSPWISPANSHCIKMLVYQYDKNGKPVRISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5N5</accession>
    <name>W5M5N5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5N5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003693"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003700.1">
        <property type="protein sequence ID" value="ENSLOCP00000003693.1"/>
        <property type="gene ID" value="ENSLOCG00000003117.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183081"/>
    <dbReference type="HOGENOM" id="CLU_018190_0_0_1"/>
    <dbReference type="InParanoid" id="W5M5N5"/>
    <dbReference type="OMA" id="NVIHERF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003117">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="423" mass="49674" checksum="B8F98F44615F75D0" modified="2014-04-16" version="1">CHKVQDSLLSSASRFTDYRGIFNWCVIMLVLTHAHLFLENFIQHGFLVDLRKVLRLLVEDGYNWPSVYLILVSNVFVATALLIEKWQEQGFLSQRTGWVLEAGNLLVLMVFPTAVILHKSTSISVVGSLLSLCFYTVLTLKIYSYHDVNSWYRQGQVGNSGSAEMKEEQEDCPGRREYRDCLTLKDLYYFLMAPTLCYQLNFPRTRTIRKNFLFQRLLEMLILIQLMVGIAQQWIGPIIQRSECTFTNMDTTAKIEHLMELVAPNHFLWLILFYLLSHSCLNFIAELLCFGDREFYGDWWNADNLKAFWSKWNIPFHKWCSRHLYRPLLRRRLAPQHAELTVFVMSAAMCEFLIAVPLQMFRLWIFIIMILEIKVAYFLGHYFTGNYGNGMVWLCMLLGPPIAVMTYFHDYYILNKENSSWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6J3</accession>
    <name>W5M6J3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Enoyl-[acyl-carrier-protein] reductase, mitochondrial</fullName>
            <ecNumber evidence="11">1.3.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">2-enoyl thioester reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.104" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01009518">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6J3"/>
    <dbReference type="FunCoup" id="W5M6J3">
        <property type="interactions" value="1695"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004001"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004009.1">
        <property type="protein sequence ID" value="ENSLOCP00000004001.1"/>
        <property type="gene ID" value="ENSLOCG00000003375.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156592"/>
    <dbReference type="HOGENOM" id="CLU_026673_17_1_1"/>
    <dbReference type="InParanoid" id="W5M6J3"/>
    <dbReference type="OMA" id="WVAPLNG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003375">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000112">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000010">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
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    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="5" id="KW-0521">NADP</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="14">
        <location>
            <begin position="38"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="360" mass="38440" checksum="0A546FF7F2D12659" modified="2014-04-16" version="1">MAKKKKKKKKMAVIASSRYSSAAAGVDCRALVYRSHGDPAQAVQMEKMKLPALGDRSVLVKMLAAPINPSDMNMVQGTYAILPPLPAVGGNEGVGQVLEVGGQVTSLRAGDWIIPRDAGLGTWRTAAVFGEEDLIAVPSDIPVLCAATLGVNPCTAYRMLSDFETLKPGDSVIQNAANSGVGQAVIQIAAARGIHTINVVRSRPDLQQLTDRLRALGAAHVVTEETLRKPAMSEIFKSCPRPRLALNGVGGKSATELLRHVQSGGTMVTYGGMAKQPVTVPVSALIFKDVRVQGFWVTKWKREHACDQGALKEMLSELCALIRGGKLSPPACTEVSFQDFRTALHSAMEPFTSAKQVLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M7Q5</accession>
    <name>W5M7Q5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019125">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019127">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019128">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019130">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M7Q5"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000004421.1">
        <property type="protein sequence ID" value="ENSLOCP00000004413.1"/>
        <property type="gene ID" value="ENSLOCG00000003712.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174784"/>
    <dbReference type="HOGENOM" id="CLU_1299365_0_0_1"/>
    <dbReference type="InParanoid" id="W5M7Q5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="212" mass="22758" checksum="CF90890863BDF61C" modified="2014-04-16" version="1">MSWRVFFSFYSECRPFTCIFFSSMNYIGLCLPDAPKNTSVSISPSGAIAEGSSVTLTCSSSANPPVKNYSWFKINGTGPLYKGPNYTIINISSADSGQYYCEAQSEIGALNSTAVNLTVAARGSSVLLAAVLGSVLLLIAVSAGTLFALRRRTAAPPGQENKREAQTEAAVDHLYGNVTQPHTPERQQSQIAPAQETEVNYSTVNFKPKTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8A0</accession>
    <name>W5M8A0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="10">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01025345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025346">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8A0"/>
    <dbReference type="FunCoup" id="W5M8A0">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004608"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004616.1">
        <property type="protein sequence ID" value="ENSLOCP00000004608.1"/>
        <property type="gene ID" value="ENSLOCG00000003859.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694445"/>
    <dbReference type="KEGG" id="loc:102694445"/>
    <dbReference type="CTD" id="169714"/>
    <dbReference type="eggNOG" id="KOG1731">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159734"/>
    <dbReference type="HOGENOM" id="CLU_020182_1_0_1"/>
    <dbReference type="InParanoid" id="W5M8A0"/>
    <dbReference type="OMA" id="VFFGCKE"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003859">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02992">
        <property type="entry name" value="PDI_a_QSOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1960:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000073">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000080">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1960">
        <property type="entry name" value="QSOX sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040986">
        <property type="entry name" value="QSOX_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042568">
        <property type="entry name" value="QSOX_FAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041269">
        <property type="entry name" value="QSOX_Trx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039798">
        <property type="entry name" value="Sulfhydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897">
        <property type="entry name" value="QUIESCIN Q6-RELATED SULFHYDRYL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897:SF7">
        <property type="entry name" value="SULFHYDRYL OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18371">
        <property type="entry name" value="FAD_SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18108">
        <property type="entry name" value="QSOX_Trx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="631"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="13">
        <location>
            <begin position="39"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="12">
        <location>
            <begin position="406"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="558"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="664" mass="74859" checksum="9B2476D333E4C016" modified="2014-04-16" version="1">MATCVSGSCSALRLPAWLVAVISFLGACILCRAQATRLYSAGDPLTILASDSLKETLHNSSTAWLVEFYSSWCGHCIQYSPTWKALAGDVKDWDQGIKLGVLDCAFEKNFDICKEFGIHFYPTFRFFKAHSTTFDVGKTYGGADRELQTMRQLMVSFLQNHSREEWPSKCPQLEPSGSADVLAQLGQKAEEYTAIIIEEEDSYVGREVILDLMKYEGVTVKRALGSEKLLLEKLGIAALPSAYLLEPSGKHGLMNVEKRLRFFFSSYLKLLPGVRRKQALSARALGPARMSGSAVAVVWKDFDKSKVYMADLESGLHYLLRVELASHQTLEGEELKTFKDFVTVVAKLFPGRQSVVKLLETLLEWLVSLPLERIPYDAILDLVNNKMRISGMFLSDQLQWVGCQGSRPELRGYPCSLWTLFHALTVQAAQRPDALANTGLEEDPQAVLQTMRRYVHTFFGCQECGRHFEEMAKESLDQVKSLQEAVLWLWRKHNQVNNRLAGALSDDPHFPKTQWPTPNLCPACHEEKDGLHVWNEEKVFHFLMDYYGAANISPKYTLSQEGPAPGRDGPSYKDKGQQDQQDIPTTNPSDPSNQRSLVGQEQGGIGVYKEAKPGVTFLGLGFSNIDMSLCVLLYAASCVFIMVMFFFFRMRSRRWKIKHNRPYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M9N1</accession>
    <name>W5M9N1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Migration and invasion inhibitory protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M9N1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005090"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005098.1">
        <property type="protein sequence ID" value="ENSLOCP00000005090.1"/>
        <property type="gene ID" value="ENSLOCG00000004256.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RZQ8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003768"/>
    <dbReference type="HOGENOM" id="CLU_061576_1_0_1"/>
    <dbReference type="InParanoid" id="W5M9N1"/>
    <dbReference type="OMA" id="CIHEQPL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004256">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031466">
        <property type="entry name" value="MIIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34831">
        <property type="entry name" value="MIGRATION AND INVASION-INHIBITORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34831:SF1">
        <property type="entry name" value="MIGRATION AND INVASION-INHIBITORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15734">
        <property type="entry name" value="MIIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="215" mass="24044" checksum="DE511C4EBD9DDAD6" modified="2014-04-16" version="1">QDAKRPKPILLTPHQERPRKEGGHVTFHSVDEEHTLSTEGLSMQPLLGYDWIAGLLDAESSLTERSEQYFAELRTFRQVNRDDCVHSLYMETEDTEVSSPARQEQDLDASLSHPGVHCYRINSRLFPVALEPQAACPVCKKPRSQGTHTIPEPAFIRVSLPRSSLLPAYRYKAHRRCSFDPSDSLGLPSHCLAGWSSTALPAAEKVNGLDLRSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MA42</accession>
    <name>W5MA42_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxynucleoside triphosphate triphosphohydrolase SAMHD1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:46149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dATP + H2O = 2'-deoxyadenosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61404"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dCTP + H2O = 2'-deoxycytidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80083"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:80084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>dGTP + H2O = 2'-deoxyguanosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61429"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:15194"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dTTP + H2O = thymidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80079"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:80080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025259">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MA42"/>
    <dbReference type="FunCoup" id="W5MA42">
        <property type="interactions" value="990"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005251"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005259.1">
        <property type="protein sequence ID" value="ENSLOCP00000005251.1"/>
        <property type="gene ID" value="ENSLOCG00000004382.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013867"/>
    <dbReference type="InParanoid" id="W5MA42"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004382">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09508">
        <property type="entry name" value="SAM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000015">
        <property type="entry name" value="Deoxynucleoside triphosphate triphosphohydrolase SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000067">
        <property type="entry name" value="SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2760:FF:000002">
        <property type="entry name" value="SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="15" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAM" evidence="24">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="25">
        <location>
            <begin position="154"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="617" mass="71422" checksum="61A16850D8A219E4" modified="2014-04-16" version="1">LENRKRLREEILPTDLCVTPEKKVSDVSWRHTDYQDWGVEETCKYLQLKGFGEWQQKFRDHQITGVGLPYITDAHLERMGIGPLGARLQILHCLRNLWQFSVDRMKVFNDPIHGHIEMHPLLVKIIDTPQFQRLRFIKQLGGTYLVFPGASHNRFEHSIGVGYLAGRLVQALYERQPELRITQRDMLCVQIAGLCHDLGHGPFSHMFDGIFIPLARPGLNWKHETASLQMFDHLVSANHLETAMEQYGLVLPDDIIFIKEQIAGPTYQNQSSNNKWPYRGRSEEKGFLYEIVANKRNGIDVDKWDYFARDCHHLGIQNNFDYKRFLKFARVCEVENKKHICTRDKEVGNLYDMFHTRNCLHRRAYQHKVGNIIETMITEAFLKADKYIQIEGSEGRTFTISTAIDDMEAYTKLTDHIFLQILYSTAPELKEARTILNNVMCRKLYKCIGQTQPKPDTKILPHELDTLASDVAKSIPQVPSDVNLDPGDFIVSVINIDYGMKEKNPINNVRFYCKNDPTNAIRIRKDQVSQLLPEKFAEQLIRVYCKKTDEKSIEAAKKYFVQWCFDRDFTKPQDGDIIAPELTPLKASWDEDSDDEGGKRVMSGVLINSKTRTKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAH0</accession>
    <name>W5MAH0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">IKAROS family zinc finger 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Ikaros C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011333">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAH0"/>
    <dbReference type="FunCoup" id="W5MAH0">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005379"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005387.1">
        <property type="protein sequence ID" value="ENSLOCP00000005379.1"/>
        <property type="gene ID" value="ENSLOCG00000004501.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686773"/>
    <dbReference type="KEGG" id="loc:102686773"/>
    <dbReference type="CTD" id="22807"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157137"/>
    <dbReference type="InParanoid" id="W5MAH0"/>
    <dbReference type="OMA" id="HFDMNLA"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004501">
        <property type="expression patterns" value="Expressed in testis and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000073">
        <property type="entry name" value="IKAROS family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000525">
        <property type="entry name" value="IKAROS family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000080">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000372">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF33">
        <property type="entry name" value="ZINC FINGER PROTEIN HELIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="357"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="541" mass="59633" checksum="C767B2E041F27099" modified="2014-04-16" version="1">MPETPQKGGVFPGAAYRDPSSNDHKTSGNLFFTFEKHKHSNGDCSPRKDHSRMVVDLSSNTPNGRHSPLGLHTNTQIKQEAGTEEEVERQALGLEEVVPGGEEGAGLEDPMIDSPNGLQDSGQGGEGPGDAGIRLPNGERPFLCNQCGASFTQKGNLLRHIKLHTGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRTSLEEHKERCHNYLQSVSMDAVPHAGLQQGDTGKEQQSMMEGMSIVPFDRPPVIERLQSNVGKRKSTTPQKFVGEKLVRYSYPEMRYDIGMKYEKDAELMQAHMMDQAINNAISYLGADSLRPLMHPQHLSMADVVPMMNPLFSQVYPPGRLERPSSREAPSANHDFSTDGPISLIRPKNPPAEREGSPSNSGLDSIDSESSSPEERHVYPGNKASQARTKSSPGYSKDDARGLEAISKAAVEAAAAAAASAASSLGPSKHSFRVFTGEGVEVKAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAJ3</accession>
    <name>W5MAJ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">CXADR Ig-like cell adhesion molecule</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Basolateral cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01003150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAJ3"/>
    <dbReference type="FunCoup" id="W5MAJ3">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000005402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005410.1">
        <property type="protein sequence ID" value="ENSLOCP00000005402.1"/>
        <property type="gene ID" value="ENSLOCG00000004512.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QSG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154829"/>
    <dbReference type="HOGENOM" id="CLU_040549_0_0_1"/>
    <dbReference type="InParanoid" id="W5MAJ3"/>
    <dbReference type="OMA" id="GTKPMQY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004512">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001618">
        <property type="term" value="F:virus receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000095">
        <property type="entry name" value="immunoglobulin superfamily member 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052307">
        <property type="entry name" value="EJ_Adhesion_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468:SF3">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="40"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="150"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="278"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="40487" checksum="AADB14C9D2F375AC" modified="2014-04-16" version="1">TESTRAQWYLTGANSFQGCGASRGLDITSTGGATLEQASGSAATLECNFTLDPTDTGPLDIEWSLLASDNQKEDKVIILYSGDRVYSDYYQPLKNRVHFVSADPKTGDASIQLTGLQSSDTGTYQCKVKKAPGIKSRKILLTVMVKPSKPRCYIEGSPQEGKDVTLKCTSAEGTKPLQYNWERTNENKLLPANAVLDRVGGTMSVKNVSASVSGTYRCLAENRVGKDDCFVHLKVVPPPNTAGIIAGAVIGVLLALILIAIILFCCCRARGKKKYEKETSYEIREDVPPPKSRASTARSFTAMSSQRSSLGSMSPSNLHEYALKGQYDQIPTEEYERPPSHVPMPPPAKVAAPNLSRMGAVAVMVPAQTRDGSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAQ8</accession>
    <name>W5MAQ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ubiquitin carboxyl-terminal hydrolase 25</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Deubiquitinating enzyme 25</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquitin thioesterase 25</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Ubiquitin-specific-processing protease 25</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homotetramer, inhibited form. Homodimer, active form. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01018527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015193275.2">
        <property type="nucleotide sequence ID" value="XM_015337789.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAQ8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005467"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005475.1">
        <property type="protein sequence ID" value="ENSLOCP00000005467.1"/>
        <property type="gene ID" value="ENSLOCG00000004503.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696022"/>
    <dbReference type="eggNOG" id="KOG1863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157670"/>
    <dbReference type="InParanoid" id="W5MAQ8"/>
    <dbReference type="OMA" id="HANQRWL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004503">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02665">
        <property type="entry name" value="Peptidase_C19I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14276">
        <property type="entry name" value="UBA_UBP25_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20487">
        <property type="entry name" value="USP28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000023">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000004">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054108">
        <property type="entry name" value="USP25/28_UIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF678">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21909">
        <property type="entry name" value="USP_UIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="25">
        <location>
            <begin position="225"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="522"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="780"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="841"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="612"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00053306"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00062193"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00078773"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00082178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1216" mass="137381" checksum="9C9E53CEBC041A75" modified="2014-04-16" version="1">MHATGTLLSPPHAVRGHARRKRARSSSWADEPRGRAWRVGDGWRRRESEAGGEGFIENYRKKRRIWSIAMTAEQQCDHGDNSGSSTHVLINQLREITGTQDVEILQRVLKASNGDMSQAITMLTAQVPEEDVIAVEPSDAEGSVVGRRAQTKVIDLTGSGDKDDLQTAIELSLQESQNAQAEDRDLNRALEASVAENKARAKRKRCEAWGETSPADWIRQDSWPVGLRNVGNTCWFSAVIQSLFHLPKFRRLVLNYTLSQSILEKCRSHSEKRNIAFMQELRCLFALMVGSSRRFVDPSAAVELLKDAFRTSEAQQQDVSEFTHKLLDWLEDAFQLAANGNNPEAKQQNPMVQLFYGTFVAERSHEGKTLSNIEQFGQYPLQVNGFNNLDECLEGAMVEGEIETLHSDQTVHSGQERWFSKLPPVLTFELSRFEFNQSLGRPEKIHKKLEFPQVIYMDRYLHRNKELIRKRRREAKKLKDQLTALQQKLESYNSYGSGPAKYPLADMLQYVLEFATTKPSSVSPAEDIRMISPPSTASPDTTTHTDPAPENTSLSEAEDSGTPDCLSSPAPPGQRTPIYKPFTQCRPLVEWPSQPAPHSVSEEELQFVEGCLQRWRAEVEQNIQELKESIDRISRQLEGMYSDASLSQVPYRLHAVLVHEGQASAGHYWAYICDHSSKLWLKYNDISVSESTWEELEKDSYGGMKNASAYCLMYIDDMLPHLIAEDTDAETGQVLGGMDSLPPVLRQYVREDNHWFQQEVEEWEEEQFRKMPQKESLIPVATDAQESSSESVPEEPPPSQQPPRSLSSEHAMIVKEQTAQAIANTADCYEKKGVEAALTEASEAETGAGGSAAEGESGADSKTEAQPQLKQPPEAGSREPESQVSEVEIPSVGKILVCSDADGYNEEMMLTPAMQGIILAIAKARQVFDKDGPEAALIKAFHEEYSRLFLLAQEKSTPQNDPRLQHVLVYFFKNDAPKRVVERTLLEQFADRNLSYDERSIGIMKEARAKLRLIRPEDMDMEEYMKWHEDYSVFRKVFVYLLTGLEQYQNGKLREALNYLVYAYQNNTALLMKGENRGMEESLIALYRRKCLTELNEAAAKLFRSGEESDVTEGVSIMNDLVIPCMHLMISNDINQEDLDAIETIRNRWCSYLGQDMNDALQEKLGEFLPRMLDCSAEIIVLKEPPKIRPNSPHDLCSRFAAVMESIHGTSIVTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCB2</accession>
    <name>W5MCB2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 7">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 7"/>
    <dbReference type="EMBL" id="AHAT01010509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCB2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006021"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006029.1">
        <property type="protein sequence ID" value="ENSLOCP00000006021.1"/>
        <property type="gene ID" value="ENSLOCG00000005000.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159757"/>
    <dbReference type="HOGENOM" id="CLU_039326_2_1_1"/>
    <dbReference type="InParanoid" id="W5MCB2"/>
    <dbReference type="OMA" id="GHTIQAN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005000">
        <property type="expression patterns" value="Expressed in intestine and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF124">
        <property type="entry name" value="CARBONIC ANHYDRASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="267" mass="30069" checksum="5494A01CEA924B6A" modified="2014-04-16" version="1">RGITKMTGHHWGYGEENGPSEWHRDYPIAMGKRQSPIDIVSSEAVYDARLSSLTVSYDSCTSLNISNNGHSVVVEFEDVDDKTVIKGGPLDAPYRLKQFHFHWGGKGSHGSEHTVNGKSYVSELHLVHWNAAKYKSFGEAASAPDGLAVLGIFLETGNEHRSLNKLTDALYMVRFKGTAAGFKGFNPKCLLPNSLNFWTYPGSLTTPPLYESVTWIVLKEPIHVSEKQMEKFRMLLFTSEEEERQRMVDNFRPPQPLNGRKVYASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCR7</accession>
    <name>W5MCR7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01022056">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022057">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01022058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCR7"/>
    <dbReference type="FunCoup" id="W5MCR7">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006176"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006184.1">
        <property type="protein sequence ID" value="ENSLOCP00000006176.1"/>
        <property type="gene ID" value="ENSLOCG00000005125.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0792">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157888"/>
    <dbReference type="InParanoid" id="W5MCR7"/>
    <dbReference type="OMA" id="GIFHIRC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005125">
        <property type="expression patterns" value="Expressed in zone of skin and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13189">
        <property type="entry name" value="FERM_C_PTPN4_PTPN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06706">
        <property type="entry name" value="PDZ_PTPN3-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14600">
        <property type="entry name" value="PTPc-N3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000104">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000023">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041783">
        <property type="entry name" value="PTPN3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012151">
        <property type="entry name" value="Tyr_Pase_non-rcpt_typ-3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF5">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000927">
        <property type="entry name" value="Tyr-Ptase_nr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="17">
        <location>
            <begin position="52"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="553"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="690"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="864"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="386"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="444"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="12">
        <location>
            <position position="887"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="856"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="887"/>
            <end position="893"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="931"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000927-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000927-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="958" mass="109090" checksum="019E54AD49B71F77" modified="2014-04-16" version="1">MVEDQGVHQQAPSLEEEGGLSTVMTSRLRVLGGRISNIRVSELPKDKSRSEVICNVHFLDGTVQSFKISKQDAGQILLDMAYNHLGLIEKEYFSLQHNEDSIDSPRWLEPNKPVRKQLKGSFPCSLNFRVRFFSPDPNTLQQEQTRHLYFLQLKNDIQEGRLSCPLSSAVVLASYAVQSELGDHSDSEHQPGYLSEFRFIPEQDEDFLTKVESLHEQHRGLKQSEAELCYLNVARTLEFYGVELHSARDPSNAEMLIGIASGGLAVFRNMICTSFFSWVNIIKISFKRKRFFVQLRQKHGETRDYSVAFSMLNYRACKNLWKSCVEHHTFFQTKKSLPQEKKLLSNYWTLGSKAPAKSINSQFCRKVIGGMVWNPVLRKSLSMEHLETKSLPSRSPPVTPNWRSPRLRHEIRKPRHSSMDNLASEMSYITETEDVFYTYRGTLGSKDSDTEAPPNHGTSRKSSESELPKKLLENSEAPTVSLEAPKNHSLQSSPSIEETQVTTTHRLSGSHPLDGLDSQFLESYDNVTRGSSTDPDSQYYCDKNEMVDGDLLLIRITPDEEGRFGFNVKGGVDQKMPLVISRVSADSPAGKCVPKLLEGDQIVLINGRDISEHTHDQVVMFIKASRESHTKELALLVRRKVVSLHVVPRPEDDDHCSLPGDPILPPCTHYGETLEESMEQLKRGLQSGTLLIQFEQLYRKKPGMAMLCAKLPQNMEKNRYKDVLPYDTTRVVLEGEEDDYINANHVNMEIPSAGIVNKYVASQGPLPHTCRHFWQAVWEQNSAIIVMLTTLTERGRTKCHQYWPDPQEVRDYGHLRVCCHSEECNIAYVFRELTLTNTETGEDRSVTHLQYVAWPDHGVPDDPSDFLDFVNFIRQKRGENDPLFVHCSAGIGRTGVLVTMETAVELIEKNQPVYPLDIVRTMRDQRAMMVQTASQYRFVCEAILRVYEEQRGRSLESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDA7</accession>
    <name>W5MDA7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nociceptin receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Kappa-type 3 opioid receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Orphanin FQ receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">G-protein coupled opioid receptor that functions as a receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01035856">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639672.1">
        <property type="nucleotide sequence ID" value="XM_006639609.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDA7"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006374.1">
        <property type="protein sequence ID" value="ENSLOCP00000006366.1"/>
        <property type="gene ID" value="ENSLOCG00000005285.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685013"/>
    <dbReference type="KEGG" id="loc:102685013"/>
    <dbReference type="CTD" id="4987"/>
    <dbReference type="GeneTree" id="ENSGT00940000160661"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_1_1"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005285">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001626">
        <property type="term" value="F:nociceptin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15092">
        <property type="entry name" value="7tmA_NOFQ_opioid_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000014">
        <property type="entry name" value="Kappa-type opioid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001420">
        <property type="entry name" value="X_opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF11">
        <property type="entry name" value="NOCICEPTIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00547">
        <property type="entry name" value="XOPIOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0085">Behavior</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="61"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="363" mass="40790" checksum="90D4B94C99A1C21D" modified="2014-04-16" version="1">MDFPNAPGGFRDPSLSRLLNDSAFNMNFSAYNYTFEGVLPIGVKITIVVLYMIVCVVGLVGNCLVMYVIIRYTKMKTATNIYIFNLALADSLVLITLPFQGTDVFLGFWPFGNVLCKIAISIDYYNMFTSTFTLTMMSVDRYIAVCHPVKALDMRTPHKAKVINICIWALASAIGIPAMVMGAAEVQLIGIECIIVLPHPDSYWEPVFGTCVFLFSFVIPVAIITICYSLMVKRLRNVRVLSGSKEKDRNLRRITRMVLIVVAVFVVCWTPVQIMVLVQSLGFILDNELTVAVMHFCIALGYVNSSLNPVLYAFLDENFKRCFREFCYPSTFRMELQQSSRMRSIAKEVAYNCKNADGTSNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDC1</accession>
    <name>W5MDC1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fatty acid binding protein 3, muscle and heart</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631365.1">
        <property type="nucleotide sequence ID" value="XM_006631302.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015204195.1">
        <property type="nucleotide sequence ID" value="XM_015348709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDC1"/>
    <dbReference type="FunCoup" id="W5MDC1">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006380"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006388.1">
        <property type="protein sequence ID" value="ENSLOCP00000006380.1"/>
        <property type="gene ID" value="ENSLOCG00000005299.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695981"/>
    <dbReference type="KEGG" id="loc:102695981"/>
    <dbReference type="CTD" id="2170"/>
    <dbReference type="eggNOG" id="KOG4015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155104"/>
    <dbReference type="HOGENOM" id="CLU_113772_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDC1"/>
    <dbReference type="OMA" id="NTEINCK"/>
    <dbReference type="OrthoDB" id="412780at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005299">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="133" mass="14840" checksum="5C7A54A26A10705F" modified="2014-04-16" version="1">MVEAFVGTWNLQESKNFDDYMKAIGVGFATRQVGSMTKPTTIISVDGDTVIVRTQSTFKNTEIKFKLGEEFDETTADDRKVKSLVTVDGGKLVHVQKWEDKETSLVREVNGNQLVLTLTMGDVVSTRTYVKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDC5</accession>
    <name>W5MDC5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">IgLON family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019546">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019547">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019548">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019549">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019550">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDC5"/>
    <dbReference type="FunCoup" id="W5MDC5">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006384"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006392.1">
        <property type="protein sequence ID" value="ENSLOCP00000006384.1"/>
        <property type="gene ID" value="ENSLOCG00000005297.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688278"/>
    <dbReference type="CTD" id="402665"/>
    <dbReference type="eggNOG" id="KOG3510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160467"/>
    <dbReference type="HOGENOM" id="CLU_027228_2_2_1"/>
    <dbReference type="InParanoid" id="W5MDC5"/>
    <dbReference type="OMA" id="GEAYGEQ"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005297">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF12">
        <property type="entry name" value="IGLON FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865992" evidence="11">
        <location>
            <begin position="22"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="27"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="126"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="210"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="333" mass="37449" checksum="38EEDCA56B898B5B" modified="2014-04-16" version="1">MSDPLLRIVLVGMLLAVLANGLYVEGAEFTHLPDNITVCQGESAILRCNIDDKVTHKAWLNRSNILFTGNDKWSLDSRVTLINNNKSEFSIQIDRVTVYDEGPYTCSFQARDEPRTAHVYLIVQVPAKIINISKDISVNEGSNVNLFCLAIGRPEPTVTWRHLKYGLLSEGEFLDITEIKRQQAEEYECITNNGVSAPDTRKVRITVNYPPVITDVKNMPAQLGKTAILRCEAMSVPTASFEWYKDEKRLMEHDSNMKIQNEKTRSLLMFTNVTAKHFGNYTCFASNRLGSSNSSMLLFRPGAAKNGAAASDFGVFMTMWFLWPALLVLLLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDE5</accession>
    <name>W5MDE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Creatine kinase S-type, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Sarcomeric mitochondrial creatine kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01012347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDE5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006404"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006412.1">
        <property type="protein sequence ID" value="ENSLOCP00000006404.1"/>
        <property type="gene ID" value="ENSLOCG00000005314.1"/>
    </dbReference>
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        <property type="component" value="Linkage group LG2"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
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        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
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        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CREATINE KINASE S-TYPE, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ATP-gua_PtransN"/>
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    </dbReference>
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    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
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    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="17">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
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        <location>
            <begin position="158"/>
            <end position="408"/>
        </location>
    </feature>
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        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="224"/>
        </location>
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            <name>ATP</name>
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        </ligand>
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            <end position="327"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <begin position="361"/>
            <end position="366"/>
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        <ligand>
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            <dbReference type="ARBA" id="ARBA00004137"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
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            <dbReference type="PROSITE" id="PS51509"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="425" mass="48199" checksum="0E6FC0F9568764F6" modified="2014-04-16" version="1">MASTFSRMLTGRKTAVLLASLGAGALATGYLVSDTNQAAHLERRKLYPPSADFPDLRKHNNCMASNLTPAIYSRLRDKVTPANWTLDQCIQTGVDNPGHPFIKTVGMVAGDEECYEVFADLFDPVIKERHNGYDPRTMKHVTDLDSSKITSGQFDERYVLSSRVRTGRSIRGLSLPPACSRAERREVERVAVQALSGLKADLAGRYYRLNEMTEQEQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNFDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVQSVCLHCSYCPHKWRQYERFNLMCACSLFLVLKWIDMALKAGENKRASSISQDPRFSKILDNLRLQKRGTGGVDTAAVGDVFDISNNDRIGRSEVELVQLVIDGVNYLIECEKRLEKGQDIKVPAPLSQFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDQ9</accession>
    <name>W5MDQ9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FEV transcription factor, ETS family member</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDQ9"/>
    <dbReference type="FunCoup" id="W5MDQ9">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006518"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006526.1">
        <property type="protein sequence ID" value="ENSLOCP00000006518.1"/>
        <property type="gene ID" value="ENSLOCG00000005405.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161562"/>
    <dbReference type="HOGENOM" id="CLU_045216_4_0_1"/>
    <dbReference type="InParanoid" id="W5MDQ9"/>
    <dbReference type="OMA" id="GFSYWAG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005405">
        <property type="expression patterns" value="Expressed in intestine and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000448">
        <property type="entry name" value="FEV (ETS oncogene family)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ets_dom"/>
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        <property type="entry name" value="ETS_fam"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF307">
        <property type="entry name" value="FEV TRANSCRIPTION FACTOR, ETS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="54"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="228" mass="25417" checksum="7CBFB738D85C6447" modified="2014-04-16" version="1">SALSDQTATMKQNCGANLMFNMYLSDPSENLFKEGKTSSWSPINTGVQKGSGQIQLWQFLLELLSDSSNMSCIAWEGTNGEFKLIDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGLAQVCQPSTTEHALYKFQSNFAPIPFSGISKLNLVTPSVAPSGFSYWPGSPPALYPGHNLQPSGAFSSVAASHISGINNINNLSNISNHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5ME90</accession>
    <name>W5ME90_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin light chain kinase, smooth muscle</fullName>
            <ecNumber evidence="5">2.7.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Telokin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
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        <cofactor evidence="2">
            <name>Mg(2+)</name>
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        </cofactor>
    </comment>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
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        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000005547.1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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            <begin position="1291"/>
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            <begin position="1425"/>
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            <begin position="1382"/>
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            <begin position="134"/>
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        <location>
            <begin position="314"/>
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        <location>
            <begin position="332"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="22">
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            <position position="1454"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006699.1"/>
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    <evidence type="ECO:0000313" key="27">
        <source>
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        </source>
    </evidence>
    <sequence length="1873" mass="207434" checksum="973A0F98DD38DFB5" modified="2014-04-16" version="1">MGDVQIVTTTRISKASLTLGRAPLSNSALPTEPPAFTLPPRNVRVALGSTARIDGKVRGYPEPQVTWYKNGKTVIAGDRNVIEQSARGTFSLVIQGVTEEDSGKYTCEAVNDGGSRQVTVELTVEGNGGRKYNLPSSSRSTGGKFSVPPVESRPSIWGESPPKFVTKPSRLILKAGQSGKFSAKITGRPQPQVMWFKDDIELQESDHFNIFEKSGIHFFEIRNLQTEDAGLYTCSVVNTAGKATASAELIVQIPNQKFTFHTTLMSPVNPVTTVTKTEMSEVKHKASNGLINEQQSAKMLEKVEPKHTYTAVTVTSRESKAVTESTAPSGKVEAEPQKPLSDHGAEIKKSSVQPKTASSVAPQELKPTLACVRAGPEGRRAGTGRRAQQGASDSGAARKMTDAEPTAPGKQRETRKSAEQQKMETRGTSPKFEIQPQSSEAVEGSEVIFKCQVSGSPKPDVNWIKDGLSLKREAGLKIHEENNMHYLCFDKVQKEDCGIYSCTATNIRGKVSITWTLNVKTPEDEGKAPFFTSALKGCSVSEGQDFVLQCTVEGKPLPQISWLLNDKTIQYAHSSFENGVAKLTVQDALPEDDGMYSCVAENSYGRAVCSAKVFIKEKQTAKKTEASNAEEQKKTFAPVFLKGLNDLQVMDGGQVSMTVEVTANPKPEIVWLHNGKEIQESEDFHFKKCGNEYTLLIQEVFPEDTGKYTCEVWNELGVARTEARLTVQEPQDGVQPWFITKPKSVTASMGQHVLLSGAIAGDPFPTFQWLKDGQRLSSGEDYEILQKEDVVSLLIRRVKPHHAGDYEIKLKNKVGDCSCLVTLLVREGAVSSEEAYQISEIEAEPSETEEEVADGTVRPGRAGAAGKSPRQTQCAEEEEHADVRGLLKTRVETKERCEEKIRQQEAEQLDFRTVLGRKVTTKSLSEEDLKELSAEQMDFRANLQRQVKPKTLSEEERKIHSPQQVDFRGVLGKKVAPKGSAPEKSEAKNAAPDFRSVLAAKKKPAAESNNNAEAEMRKPRADKEKEKNEVNCEHGCPAVDGGIIEKKNNNIGKEPVFTKKLCDVKVVDGECLRLQCQLDSDPPAVVTWSLDGKVIKPSKFIVLSQEGGLCSITIDKALPEDEGQYKCIAENSAGKAECSCTVLVDDPSATNSSSPADKKTKKTKSTTPTTESESTYEATVKKKPAPKTPPKIAVPPQILQFPADMKIRAGEPVDIFCKFGGTQPITCTWLKFRKQIQEKGPIKIEITQTASKLTISPTKHEHCGCYTLQLENKYGSRQAQVSLTIVDKPEPPAGVPCTSDIRSSTLTLSWYGPTYDGGSAVQSYTIEIWNSVDKQWKDLTSCRSTSFNVQGLLPDREYKFRLRAANIYGVSEPSQESELVTVGEKTEVSEKKEEAEISDDDADKEAEPEYRDVFINTAQKVQELYDVDERLGTGKFGQVFKLFEKKTGKVWAGKFIKAYSAKDKENVRQEISIMNCLHHPKLVQCVDAFESKSDIVMVMEMISGGELFERIIDEDFELTEREVIKYMLQIIDGVRFIHKQGIVHLDLKPENIMCVNKTGSKIKLIDFGLARRLAENSSSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLSNVTSATWDFDDEAFDEISDDAKDFISSLLKKDMKRRLTCDQCQQHPWLKQDTKNMEAKKLSKERMKKYLLKRKWQKTGHAVRAISRLSSMAMISGLSGKKGGSPTSPPISDRLETEEDVAQEFLESVVEERPHVKPYFSQVIKDVEVVEGSAARFDCKIEGYPDPVVVWYKDDQPIKETRHFQIDYDEEGNCCLVISDVSGDDDAKYTCKAVNSLGEVTCTAELIVEVMGEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEI4</accession>
    <name>W5MEI4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639685.1">
        <property type="nucleotide sequence ID" value="XM_006639622.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEI4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006801.1">
        <property type="protein sequence ID" value="ENSLOCP00000006793.1"/>
        <property type="gene ID" value="ENSLOCG00000005626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687862"/>
    <dbReference type="KEGG" id="loc:102687862"/>
    <dbReference type="CTD" id="334728"/>
    <dbReference type="GeneTree" id="ENSGT00490000043329"/>
    <dbReference type="HOGENOM" id="CLU_041744_1_0_1"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005626">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000001">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="13">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="14">
        <location>
            <begin position="183"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="29696" checksum="517F8BBD3AE45B0C" modified="2014-04-16" version="1">MAVNVYSTSVTSDNLSRHDMLAWINESLKINLTKIELLCSGGAYCQFMDMLFPGCVPLKKVKFQAKLEHEFIHNFKLLQASFKKMGVDKIIPVDKLIKGKFQDNFEFVQWFKKFFDANYDGKDYDPVAARQGQETMPLPNPSTPALNKPKKIPSTGSAAPQRPAAVAKPAPKVASVGARRPAGAGNGDEEKAELVQEVNLLKLTIEDMEKERDFYFGKLRNIELICQEKEGENDPTLQRIVDILYATDEGFVIPDAESQEQEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGT7</accession>
    <name>W5MGT7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phospholipid phosphatase 7 (inactive)</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01035497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006640848.1">
        <property type="nucleotide sequence ID" value="XM_006640785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGT7"/>
    <dbReference type="FunCoup" id="W5MGT7">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000007605.1">
        <property type="protein sequence ID" value="ENSLOCP00000007596.1"/>
        <property type="gene ID" value="ENSLOCG00000006288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683686"/>
    <dbReference type="KEGG" id="loc:102683686"/>
    <dbReference type="eggNOG" id="KOG4268">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157147"/>
    <dbReference type="HOGENOM" id="CLU_072573_4_0_1"/>
    <dbReference type="InParanoid" id="W5MGT7"/>
    <dbReference type="OMA" id="FVSFMLN"/>
    <dbReference type="OrthoDB" id="10266771at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG21"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006288">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF17">
        <property type="entry name" value="INACTIVE PHOSPHOLIPID PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="3">
        <location>
            <begin position="157"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="291" mass="32330" checksum="0EC09385D56F3FE9" modified="2014-04-16" version="1">MPAHQSRSRTRDRNNVLNRPEFMSLNQPLRNSPAEVRSSLRRHNVQAGQAKHTQVPQPDYPTQEPSDNVKDRRESLKLPEDDCMQLNPSFKGIAMNSLLAIDICMSKRLGVCAYSTSSWGSVRSMVTLLAITGHGIPWICGTLLCLTRSSTLAGQEVLVNLLLALVLDVMTVAGVQKLVKRKGPWEISPSFLDYVAMDVYSFPAAHASRAAMVAKFLLNHLVLAVPLRILLVIWAFLVGVSRVMLGRHHLTDVGCGFALGFLHYYLVEMVWLSSNTCQTLISISTLSPFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHE7</accession>
    <name>W5MHE7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Exosome complex component 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Component of the RNA exosome complex. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6 (via its N-terminus). Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. The RNA exosome complex interacts with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with MTREX; the interaction with MTREX mediates the association of MTREX with nuclear RNA exosomes. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with ALYREF/THOC4. Interacts with DHX36; this interaction occurs in a RNase-insensitive manner. Interacts with NRDE2. Interacts (via C-terminus) with USP36 (via C-terminus); the interaction is facilitated by the association with RNA and promotes sumoylation of EXOSC10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHE7"/>
    <dbReference type="FunCoup" id="W5MHE7">
        <property type="interactions" value="1748"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007806"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007816.1">
        <property type="protein sequence ID" value="ENSLOCP00000007806.1"/>
        <property type="gene ID" value="ENSLOCG00000006443.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015408"/>
    <dbReference type="HOGENOM" id="CLU_010129_1_1_1"/>
    <dbReference type="InParanoid" id="W5MHE7"/>
    <dbReference type="OMA" id="NIMRPQM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006443">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019219">
        <property type="term" value="P:regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000022">
        <property type="entry name" value="Exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="13" id="KW-0271">Exosome</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="HRDC" evidence="24">
        <location>
            <begin position="507"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="737"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="784"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="737"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="829"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="862"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="898" mass="102852" checksum="9B1A6BC508769E0D" modified="2014-04-16" version="1">KMAASTSRSGRGKGNNISPTNEEAEGEKPELLPGFKDADSFVKHGLGTVVAATKASANLPQAGDEFDFYRSFPGFQEFCETQGERLLQCMSQLMQYHSCKSHMRDRNKVTGLEERFDLLVDSNDVILERVGILLDEASGVNRNQQPILPAGIQPPKTIVSSWNRKSRDSSKAAKSETFRLLHAKNIQRPQLKFREKVDNSNTPFVPKIFVKPNALKSLPTYFTDQKIRKERPEDLDVPPALADFIHQQRTQQHLDDMFAHPYQYELDHFTVPENLLSKPEPQMYKPLAETNCQFINSMEDLVELNEKLAKCSEFAVDLEHHSYRSFLGLTCLMQISTRDEDFIIDTLELRSEMYILNETFTDPSIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARVLNLGRHSLDHLLKLYCGVDSDKQYQLADWRIRPLPEEMFQYARADTHYLLYVYDRLCKELYECGNEQPALLQMVWQKSRDVALKKYTKPIFTEESYLELYRKQKRMFNVQQLAALRLLFAWRDKLARQEDESTGYRYVLPNHMLIKISEELPREPQGIIACCNPVPPLVRQQVNELHLLVQQARETPLLKTEPVTSEKKKGFTLKRKSKNVLFGPHDDSHVSEDDFPDFTSHGAPVKQGALFSEETGASDDWSSLLASAKITIFEHSGTDTEKSGYLTVAQQKAHSILASFENPFRMYLPSKDNNIHLSENAKYDPSSKVYEISNRWKLLSQEQQKKDAEAKEEARKKAREAAQKAAEERKKAQKNYKESLQNVVTVRQQAVMAAAEQEGKKRQPNPSESGAEIPKPERKRPKATEQPAQQGAPVPADFKPFDYSKSDFKVFAGGKSKESTQFDPNKQAQEPKRKKNPKGHRMNSAGNKSMSYLAGKSERGFRHNWPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHW0</accession>
    <name>W5MHW0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01019756">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019757">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019758">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHW0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007979.1">
        <property type="protein sequence ID" value="ENSLOCP00000007969.1"/>
        <property type="gene ID" value="ENSLOCG00000006600.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154947"/>
    <dbReference type="HOGENOM" id="CLU_000288_5_16_1"/>
    <dbReference type="InParanoid" id="W5MHW0"/>
    <dbReference type="OMA" id="RHHIQSF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006600">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14215">
        <property type="entry name" value="PKc_CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000145">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000061">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF6">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="31"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="43637" checksum="3182E18A934CA1BD" modified="2014-04-16" version="1">QSNSRTRAQSIRDDEEGHLMYRSGDVLQERYEIVNTLGEGTFGRVVQCIDHRRGGARVALKIIKNVEKYKEAARLEINVLERINEKDPSPPSLCVQMYDWFDYHGHMCISFELLALSTFDFLKENNYLPYSITQVRHMAYQICQAVKFLHDNKLTHTDLKPENILFVNSDFTMTYNVEKKRDERTVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCILFEYYLGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYHGRLDWDDSSSAGRYVRENCKPLRRYMLSEAEEHHLLFDLVGSMLEYEPNKRLTLAESLRHPFFSPLRGGAGEAPGDCAAGKAWEGNRDISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHZ3</accession>
    <name>W5MHZ3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 38 member 8b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020935">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHZ3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008002"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008012.1">
        <property type="protein sequence ID" value="ENSLOCP00000008002.1"/>
        <property type="gene ID" value="ENSLOCG00000006623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685976"/>
    <dbReference type="KEGG" id="loc:102685976"/>
    <dbReference type="CTD" id="100004247"/>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157764"/>
    <dbReference type="HOGENOM" id="CLU_038973_0_0_1"/>
    <dbReference type="InParanoid" id="W5MHZ3"/>
    <dbReference type="OMA" id="QDFWKRS"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006623">
        <property type="expression patterns" value="Expressed in embryo and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000038">
        <property type="entry name" value="Putative sodium-coupled neutral amino acid transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF226">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="28"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="445" mass="48753" checksum="FCE8CBF65CF83D4F" modified="2014-04-16" version="1">MEELARESISLMAKPALDTDGPKLSSSGAVFIMLKSALGAGLLNFPWAFERAGGISTAVAVEMVSLVFLISGLVILGYSSSISGQSTYQDVVRALCGLAVGKLCEICFVFNLFMISVAFLVVVQDQLEKLCDSLYLTVTATAGAEVPHHWYMDQRFALFILCVVVILPLSIPKEIGFQKYTSILGTLAATYLTVVIIVKYCMTEHSGVIYTPNQSTRIGSWASMFSVIPTICFGFQCHEACIAIYSSMENKRISHWVIISVTSMFFCLLIYTLTGVFGYLTFGRNVAADILMSYPGSDVVIIVARLLFGVSIITIYPIILLLGRSVIQGLFLRYRRQRTVVTQSYENRTRVVLTVAWIAVTLIIAMFMPDISKVISVIGGISAFFIFIFPGLCLIFAMQSASVSETVSPRTRLALSAWGVLSVLCGAFIFGQSTTIAIMELFNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHZ4</accession>
    <name>W5MHZ4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zgc:153867</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01018906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018907">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01018908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHZ4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008003"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008013.1">
        <property type="protein sequence ID" value="ENSLOCP00000008003.1"/>
        <property type="gene ID" value="ENSLOCG00000006627.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234570"/>
    <dbReference type="HOGENOM" id="CLU_061288_13_0_1"/>
    <dbReference type="InParanoid" id="W5MHZ4"/>
    <dbReference type="OMA" id="XHEAFLL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006627">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF7">
        <property type="entry name" value="SIMILAR TO MYOSIN, LIGHT POLYPEPTIDE 6, ALKALI, SMOOTH MUSCLE AND NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="1" id="KW-0518">Myosin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="210" mass="23074" checksum="2559EF9F31614C75" modified="2014-04-16" version="1">MAPKRESSVKKPASGTPPPAPARLGPPFPRPGPALPRPVPPLRPRLLKPSDCPFDPAAVVSDFSEDQILEFKEAFMLFDRTGDGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNHKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLAGHEDANGCINYEVFVRHIMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MI78</accession>
    <name>W5MI78_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MI78"/>
    <dbReference type="FunCoup" id="W5MI78">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008087"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008097.1">
        <property type="protein sequence ID" value="ENSLOCP00000008087.1"/>
        <property type="gene ID" value="ENSLOCG00000006694.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158805"/>
    <dbReference type="HOGENOM" id="CLU_055524_10_1_1"/>
    <dbReference type="InParanoid" id="W5MI78"/>
    <dbReference type="OMA" id="HETLSCC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006694">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03151">
        <property type="entry name" value="CD81_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF214">
        <property type="entry name" value="CD81 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="217" mass="23986" checksum="7D0942A0DE0D8630" modified="2025-10-08" version="2">MAGGVILGVALWLRHDNDTKSLLDLKLEGDEAPNTFYIIRVYILIAVGAVMMFVGFLGCYGAIQESQCLLGTFFACLVILFACEVAAGIWGFINKDQISKEMIKFYDTAYDRATLDTLVQEEKKGAKEVLKVFHETLQCCGKGAATAFVGMVFSDICPKTSVQDVITNVNCHDRIRELFSTKLYLIGIAALIVAVIMIFEMIFSMVLCCGIRNNSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIA5</accession>
    <name>W5MIA5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidoglycan recognition protein 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5MIA5">
        <property type="interactions" value="807"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008114"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008124.1">
        <property type="protein sequence ID" value="ENSLOCP00000008114.1"/>
        <property type="gene ID" value="ENSLOCG00000006717.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR3D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158718"/>
    <dbReference type="HOGENOM" id="CLU_038892_0_0_1"/>
    <dbReference type="InParanoid" id="W5MIA5"/>
    <dbReference type="OMA" id="MDCPPII"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006717">
        <property type="expression patterns" value="Expressed in liver and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF66">
        <property type="entry name" value="N-ACETYLMURAMOYL-L-ALANINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="2">
        <location>
            <begin position="313"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="470" mass="51305" checksum="712ACF566F0F9759" modified="2014-04-16" version="1">LDQACVLESTVRKELLLLPVTSGLLSWHLDDVIRAVELTERSNPGLDMLGIVRTLRSTANHDDSFLRHFLGPLPDGGPPARTDEATLAPAWDHTGFVAGMFRHRVTAGGQEEGVVLTPDGTTTAVAPMLLGIEAGLQRKRGAPESGIPDALYSVTLAKDLGLSFVRFSNSQLAGRLGPDGCWDSLTAPRNFTLSGPPALATDALVNGAMDGVVLGDHLSREEDPPKLSQLLKLYFHHNGLALDSAPRLQSNFRRGNFGKLANATFLQEQIVASLSLYRGLMGDALLEGLDSAALESAVREGLPEFVHRYMVCPAIIPRCMWGAEPYRGTPTPLVLPLSFLYIHHTYGPSLPCLTFPDCARDMRAMQRFHQDDRGWDDIGYRXHNSLGYGVSFIGDYTATLPARRAMALVRERLTQCAVDGGRLVSNFTIHGHRQLVNTSCPGDKLYTEITGWEHFREVQPKPPGDSRAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ35</accession>
    <name>W5MJ35_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Opsin-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">G-protein coupled receptor 136</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">G-protein coupled receptor PGR12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Neuropsin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01034844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034846">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034847">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000008404.1">
        <property type="protein sequence ID" value="ENSLOCP00000008394.1"/>
        <property type="gene ID" value="ENSLOCG00000006934.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271854"/>
    <dbReference type="HOGENOM" id="CLU_009579_3_0_1"/>
    <dbReference type="InParanoid" id="W5MJ35"/>
    <dbReference type="OMA" id="YETFEVG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006934">
        <property type="expression patterns" value="Expressed in testis and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005502">
        <property type="term" value="F:11-cis retinal binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008020">
        <property type="term" value="F:G protein-coupled photoreceptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007604">
        <property type="term" value="P:phototransduction, UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000104">
        <property type="entry name" value="Opsin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="48"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="315" mass="34443" checksum="B052E5F81239BAFD" modified="2014-04-16" version="1">FQAMPRDGNSSTEGALTLPHPFLSRLSPSADVGIAVYLSLTGVLSVAGNGVVLTVFSRKRSHLRPQELMAMNLAVCDFGYSLLGAPFLISSSLAHSWMWGHAGCVWYGVQGFVFGIGSLITTALISLDRCLKICSIRYXGQWVERRHIGLLIVGVWLYTVTWALLPVFGFGSYGPEPFGTSCTIDWWRMRFSRTDRLYVCLIMTLCFGLSTLTIAGSYLAILIQVRRSGRSLAAIPSSAVTHFSKEMRLTKIAAVICSTFLLAWTPYVIVSLYSALGGIKGLSSLPPEVSVIPALLAKSHCVINPFIYQAMSKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ43</accession>
    <name>W5MJ43_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iroquois homeobox 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJ43"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008412.1">
        <property type="protein sequence ID" value="ENSLOCP00000008402.1"/>
        <property type="gene ID" value="ENSLOCG00000006946.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692643"/>
    <dbReference type="KEGG" id="loc:102692643"/>
    <dbReference type="CTD" id="140746"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165935"/>
    <dbReference type="HOGENOM" id="CLU_060342_0_0_1"/>
    <dbReference type="InParanoid" id="W5MJ43"/>
    <dbReference type="OMA" id="HHRYIAN"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006946">
        <property type="expression patterns" value="Expressed in ovary and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060059">
        <property type="term" value="P:embryonic retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072498">
        <property type="term" value="P:embryonic skeletal joint development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021575">
        <property type="term" value="P:hindbrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032330">
        <property type="term" value="P:regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF41">
        <property type="entry name" value="IROQUOIS HOMEOBOX 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="115"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="117"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="35642" checksum="FA38A1D68AC204FD" modified="2014-04-16" version="1">MSSGEGVPRRCRSADTGKTRMPASQTGFGDLFLGKNLRLPPGNQTVMVPPPPAFPALAAAPLFFPGVQDCGFVPYPHLRHGAHRSCFDPKAVSLYHGALLSRAGALYSTYRPLAAEDPRAGKAATRESTGALKAWLNEHLKNPYPTKGEKVMLAIVTKMSLTQVSTWFANARRRLKKENRMSWGSKGRSEEEEEEEEEDSEGEEGLGPKGPCSGDEQEDPRAVKDAEADAGGLPGSERVPPAPGTESDTEAVCARSGLGCTENEPKAQKSKIWSLAETATSGGGHRARSTSCGPDTGALWPDWALRNGQYSPVYYPAQYIFKHGPASGLNCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJ68</accession>
    <name>W5MJ68_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01040439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040440">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01040443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008427"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008437.1">
        <property type="protein sequence ID" value="ENSLOCP00000008427.1"/>
        <property type="gene ID" value="ENSLOCG00000006959.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1077">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182838"/>
    <dbReference type="HOGENOM" id="CLU_003824_1_0_1"/>
    <dbReference type="InParanoid" id="W5MJ68"/>
    <dbReference type="OMA" id="ILYEDQY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006959">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1230:FF:000003">
        <property type="entry name" value="AP-2 complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000004">
        <property type="entry name" value="AP-2 complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003164">
        <property type="entry name" value="Clathrin_a-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02296">
        <property type="entry name" value="Alpha_adaptin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="4 8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="11">
        <location>
            <begin position="687"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="920" mass="102200" checksum="CD5B6F6B938AFD93" modified="2014-04-16" version="1">KSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLSSRNPTFMNLALHCIANVGSREMAEAFAADIPRILVAGDTMDSVKQSAALCLLRLNRTSPDLVPMGEWTSRVXTTLAQKNPEEFKTSVSLAVARLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAIRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKVRPGPARAWAVPPTHRAPVTWIPGPAVTEQMCYCALSGWTVQSGLCLQALGCCWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLVQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEIKGTIQDVLRSDSQLKNADVELQQRAVEYLRLSCIASTDILATVLEEMPPFPERESSILAKLKRKKGPGMLPDIEDGRKERNADVNGGAEPVHVSTNSKVPCACVLCCAPPGTHALSPCPFVPEFPQDLCQQQTQILVLASRTNAPTLNSAPPPAAGGSLLVDVFSENSTSLPMMPPADENFSRFVCKNNGVLFENQLLQIGLKSEFRQNLGRMYVFYGNKTSTQFLSFSSSVLCQDSLKVPQLNVHTKPVDPTVDGGAQVQQILNIECVSDFMEAPVLNIQFRYGGTLQNIAVKLPITLNKFFQPTEMTSQDFFQRWKQLSAPQQEVQKIFKAKHSMDTEVTKAKIIGFGVALLDRVDPNPSNFVGAGVIHTKTTQVGCLLRLEPNTQAQMYRLTLRTSRDTVSQRLCELLSDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKZ8</accession>
    <name>W5MKZ8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKZ8"/>
    <dbReference type="FunCoup" id="W5MKZ8">
        <property type="interactions" value="1374"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000009057"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009068.1">
        <property type="protein sequence ID" value="ENSLOCP00000009057.1"/>
        <property type="gene ID" value="ENSLOCG00000007462.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694302"/>
    <dbReference type="KEGG" id="loc:102694302"/>
    <dbReference type="CTD" id="51236"/>
    <dbReference type="eggNOG" id="KOG2973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016546"/>
    <dbReference type="HOGENOM" id="CLU_037769_3_0_1"/>
    <dbReference type="InParanoid" id="W5MKZ8"/>
    <dbReference type="OMA" id="MCILLTN"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007462">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="4">
        <location>
            <begin position="275"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="380" mass="43229" checksum="8B749590AD5FE0DA" modified="2014-04-16" version="1">MLNDKEAQDLLGFLTPEMRPDVKGQATAYILGLTGTKDGCRYLRGKPDFLKALLSLTSDASVAVVKDCYHALINLSADETLHEPMVKEANVLPRLFHNLLDPEYAFADRICTILTNLSRHEKTCKAVFRVLQEEGIGLAKIVEVFCTEGYNAKASLHYLGPLLSNLTQLPETRQFILDKNRCVVQRLLPFTQYEASTVRRGGVVGTLRNCCFDYAYHEWLLSDAVDILPFLLLPLAGPEELSEEENEGLPVDLQYLPEDKKREEDPDIRQMLIETVLLLTATKTGRQILKDKNVYPIMREFHKWEKDPHVVSSCEKLVQVLIGDEPEPGMDNLLEVNIPEDVEEKLRLWDAKEQEQLEKEKEEFLKTGCEGRAEIGEQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MM94</accession>
    <name>W5MM94_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hemoglobin cathodic subunit beta-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the globin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013667">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MM94"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009503"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009514.1">
        <property type="protein sequence ID" value="ENSLOCP00000009503.1"/>
        <property type="gene ID" value="ENSLOCG00000007827.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693569"/>
    <dbReference type="KEGG" id="loc:102693569"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157809"/>
    <dbReference type="HOGENOM" id="CLU_003827_10_0_1"/>
    <dbReference type="InParanoid" id="W5MM94"/>
    <dbReference type="OMA" id="NEMVANH"/>
    <dbReference type="OrthoDB" id="9886081at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007827">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015671">
        <property type="term" value="P:oxygen transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08925">
        <property type="entry name" value="Hb-beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000001">
        <property type="entry name" value="Hemoglobin subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002337">
        <property type="entry name" value="Hemoglobin_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF7">
        <property type="entry name" value="HEMOGLOBIN SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00814">
        <property type="entry name" value="BETAHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="147" mass="15999" checksum="BF04A2597265552A" modified="2014-04-16" version="1">MVHFTDAEAKAIKTVWGKVNVDIVGPQALARLLIVYPWTQRYFGAFGNLTNAAAILGNAKVAHHGKVVLGALDKAVHDLEHIVANYASLSELHSTKLHVDPDNFRVLGDCITIVLAAQLGTGFTVELNAAFQKFLDVVIAALRKQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPX6</accession>
    <name>W5MPX6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin carboxyl-terminal hydrolase CYLD</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Deubiquitinating enzyme CYLD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Ubiquitin thioesterase CYLD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Ubiquitin-specific-processing protease CYLD</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="29">Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and RIGI. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with MAP3K7. Identified in a complex with TRAF6 and SQSTM1. Interacts with OPTN and SQSTM1. Interacts with CEP350. Interacts with RNF31; the interaction is indirect and is mediated via SPATA2. Interacts with SPATA2 (via the PUB domain); the interaction is direct and recruits CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="AHAT01010059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01010061">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPX6"/>
    <dbReference type="FunCoup" id="W5MPX6">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010435"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010449.1">
        <property type="protein sequence ID" value="ENSLOCP00000010435.1"/>
        <property type="gene ID" value="ENSLOCG00000008578.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3556">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164912"/>
    <dbReference type="HOGENOM" id="CLU_003910_0_0_1"/>
    <dbReference type="InParanoid" id="W5MPX6"/>
    <dbReference type="OMA" id="KYYFHCA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008578">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02670">
        <property type="entry name" value="Peptidase_C19N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000021">
        <property type="entry name" value="Cylindromatosis (turban tumor syndrome), like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000009">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase CYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="24" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0645">Protease</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="22" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="21" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CAP-Gly" evidence="31">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="31">
        <location>
            <begin position="307"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="30">
        <location>
            <begin position="405"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00030882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00031094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00032487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00046580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="763" mass="85678" checksum="82A7B2D2FB071AD3" modified="2014-04-16" version="1">SKGKYLYFICVEKTHNSFIPGSICYIEERRYLEKFENDSNPEHLPVMAMGSMVSYHVKVMALRHLSETEAGLLQAIVEDAQRLRYFLHRDELDSALALKIDAPVSVEIRSEWCQGILRYIGSITSIPALYTSHMLPGIFFGIELQGNDKGKGEYDGTYMSRTFFCCEKNCGIFAPFNRVRPGRLRTSWPQPLTSRKPPAASAEPLNPGDRVQVFLADDSFRQGVVIALKKEEAGMCAIISMKKGEEEGEKQERLTVPLECAMKQELLSPPVWSLDGDEKHFSLSLNSVVEVSLSEGRTVYGTVRWIGRLPDSEGIRVGLELEEDKGVSDGTFKGRRLFQCPPKRGLFVRQSSCRPDSRFQPPGKRDGKASGWHTVQGSVNSHAVPSTVAPLWGDQAVQVLAGQMKGIQGHCNSCYMDATLFSLFSCSSVLDSLLFKSAQPSDALIQATLLREIVNPLRTEGYVEASSVMKLRKQLMEGGHCASFTTDEKDPEEFLNLVMHHILALDPLLKLKTGEQKVQDGYCYQIFIDQNNSLALPTVQQLLEHSFHSALLRLAEVPSCLILQMPRFGKKFKLFDKIIPSLELDITDLLSDSPQECVLCGELATMECAECFRDATFSNTGFKQFCYTCSKQVHSLPRRQKHVPSPLLLPEGYPTGKYLRPPREQLELFAVLCIETSHYVSFVKYGPSSQDWIFFDSMADRYGDEDGYNIPAVQLCPEVGQYLEMPLGELAAVCPRDMEGVAKRLFCDAYMYLYQSPSMALYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQP2</accession>
    <name>W5MQP2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 131 member Ab</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM131 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034288">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQP2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000010701"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010716.1">
        <property type="protein sequence ID" value="ENSLOCP00000010701.1"/>
        <property type="gene ID" value="ENSLOCG00000008790.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR3P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183106"/>
    <dbReference type="HOGENOM" id="CLU_074265_0_0_1"/>
    <dbReference type="InParanoid" id="W5MQP2"/>
    <dbReference type="OMA" id="ACISQVV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008790">
        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026782">
        <property type="entry name" value="FAM131"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736:SF4">
        <property type="entry name" value="PROTEIN FAM131A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736">
        <property type="entry name" value="PROTEIN FAM131B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15010">
        <property type="entry name" value="FAM131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="311" mass="34036" checksum="1784D9FF422CA06E" modified="2014-04-16" version="1">VNVEDTVEMLPKSRRALTIQEIAALARSSLHGISQVVKDHVTKPTAMAQGRVAHLIEWKGWCKPTDSPAALESDFNSYSDLTEGEQEARFAAGVAEQFAIAEAKLRAWSSIDGEDSNDDSYDEDFGPASEPSQTLQNSDTVSSYPSYLRDFLHSHVCQQRSSRQGSSEQENSDTSPTVSPDTLCSSLCSLEDHPLLKDLGSSPVDLAAKILGALQGGEELLSRLQRGRESAFRSLGHLVCEDSSYSVSYTESCFSPEDEEDLACKDYDSVCKDECDYPGRRKVSDVASSGVVSLDEEEEEEEQEEGEEGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRP2</accession>
    <name>W5MRP2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDRD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01032268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032269">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032270">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01032271">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRP2"/>
    <dbReference type="FunCoup" id="W5MRP2">
        <property type="interactions" value="703"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011051"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011067.1">
        <property type="protein sequence ID" value="ENSLOCP00000011051.1"/>
        <property type="gene ID" value="ENSLOCG00000009071.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159902"/>
    <dbReference type="InParanoid" id="W5MRP2"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009071">
        <property type="expression patterns" value="Expressed in testis and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF19">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HTH OST-type" evidence="10">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="10">
        <location>
            <begin position="123"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="10">
        <location>
            <begin position="370"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="309"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="711" mass="79972" checksum="E97BE4D82B59AC7B" modified="2014-04-16" version="1">MSKEQLLDTLKKDVRSLLISAKAGLAPEHLKRDYTAMLGHPLPLQRLGFCSVLDMAVHMPDVVCLESTKDGSTMLKAIGVDSTKGIEELVAKQRDSKPKAFKKKAHCVGSQAPVLLHRRGNPPPSLPASLRVELRQLLVHGPLRLSELELQFATRFSRPLQISRYGFRSISEVLRAAEDMIEVKQSRTGSVLSLKRSKMAARVQPLHDPGMSELTFFVCLKTLGNLKLYEVLMFVMGGLPQAENVNTYKKLPNKGTSQQTKVVVSSSIGHLSANFILCDGMTNYKNKSQTIREFSPALNWTANLTVKNSTGEDTASRRTAKEEKGLSASQLQSTPSMQQSPAISQDEKLEENLKNTLLDCVQAGTINPQLKEKLRQDIMHVVVSQKPEGVLVQSLPGEFKKMFGEDLPVSQCGFLSVTDLVSSLSDTLHLTMAGEKWCILKSKNVRLLNEFTIGFKYRCSHVLLWASIYEKQTFLNKCYQVYSKCKHFLKWIKKHIKETQVEEPKIPPDAMQSGCLQPAARRQEETFVPVLVEKVVSPSQFYIRFSDTHEARALENMMFQMRCCYSSPEVAKRYQMPEPFVRVGQVGCVAPSGLWFYRVVIHRIVSDLKVEVYCVDFGGLNCVEKSNLKFLKLCYSTLPAQAVPSTLTWIKPSQGSWSEGAVGYFKKLCCERPMIAVIYCYMKDVLHLFLCDTHTSEDLYIHNAMKMEGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSR5</accession>
    <name>W5MSR5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nectin cell adhesion molecule 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01013865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01013866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MSR5"/>
    <dbReference type="FunCoup" id="W5MSR5">
        <property type="interactions" value="1065"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011424"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011441.1">
        <property type="protein sequence ID" value="ENSLOCP00000011424.1"/>
        <property type="gene ID" value="ENSLOCG00000009367.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTRU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156028"/>
    <dbReference type="HOGENOM" id="CLU_029618_3_0_1"/>
    <dbReference type="InParanoid" id="W5MSR5"/>
    <dbReference type="OMA" id="FWVQDPP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009367">
        <property type="expression patterns" value="Expressed in ovary and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098631">
        <property type="term" value="F:cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000298">
        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000378">
        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="535" mass="59464" checksum="5E112B4AC3E1D1AA" modified="2014-04-16" version="1">IGMNSSKKDHRLEAKSVLVFNFSLCLSVFLGVGSNEVIVDPRVTAVLGKNITLMCTVEVGRNLSLTQTSWERKHNGSTLTLAVFNPIYGISIAEDYKKRLFFKDPSNEDATIVLEDVGFADTGVYTCKVATFPLGNTQASTMVTVMVEPKVYVSAGSAPLIDGGNETVVATCTAERGKPRAEVSWESDLFGRPVVKYIDEANGTSTMEVQYRWKPTRLVQGRKLTCVVRHPALETEFRIPYVLNVQYAPVIQIKGYDNNWFVGRENVQLVCQADANPPPARYIWTRLDKDLPPGVQIVNGTLSFNRALDRNDSGIYQCDVANDIGHQHKQMDISIQEPPPTTTTPPTTPPPLPTAAATILVATKRVQFTSPTLESLPEGNLGTIIGGAVGGALFLILLIVLVGVCYMRKRRTFRGDYYTKQYIGPSDMQKESQLDVLQPHELEMYNEPSKGDIKPKSNDGIYPEFSKEMKNDEWGHVDNMNNRCTERDAVPVSYYGDSRGPSGNTYLPDDYYEGNDSDYVSHLDGSVISRREWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT03</accession>
    <name>W5MT03_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
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            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="gene ID" value="ENSLOCG00000009429.1"/>
    </dbReference>
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    <dbReference type="OMA" id="MIGSSIC"/>
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        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009429">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_opsins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
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    <keyword evidence="7" id="KW-0807">Transducer</keyword>
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    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868236" description="G-protein coupled receptors family 1 profile domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="317"/>
        </location>
    </feature>
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            <end position="31"/>
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    </feature>
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        <location>
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            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="22"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="317" mass="35866" checksum="F6A9C8EE6EFC2C31" modified="2014-04-16" version="1">MPLIALLYALAMITTSIGSVCGNVMLLLVVAFNTKLQTETWALTLNFCICDLILGLSAIPFGIHNSLSGVEGYDTKSILCQSNAFVYVLLQLASVHSLTWATIDKFTEICFALQYAQLFTKKRTWVILSFLWMYCLLNACFPFMGFGSYRYSENKFICMPRFQPSSKSYSIIIIVIGVIIPIMIMCSLYIYIVYIARNQALRGTFVCNDQHCYYVPANNYIRSSIVMISTAVTSHNSDHAGSSNKNLRKVCLLVCWMPYITVSFFETFTEYSIPPVADAVATWLVLLTSALNPWVNSMTQKKYRKALRGSLKKLRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MT27</accession>
    <name>W5MT27_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Collagen type VIII alpha 1 chain</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006639244.1">
        <property type="nucleotide sequence ID" value="XM_006639181.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015364025.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000011553.1">
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        <property type="gene ID" value="ENSLOCG00000009451.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684482"/>
    <dbReference type="KEGG" id="loc:102684482"/>
    <dbReference type="CTD" id="557795"/>
    <dbReference type="eggNOG" id="ENOG502QRFR">
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    <dbReference type="HOGENOM" id="CLU_001074_21_0_1"/>
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</entry>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
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                <person name="Jaffe D."/>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="201" mass="21538" checksum="5907C021F1FD3309" modified="2014-04-16" version="1">MQSDEQDGFTMVLDESCGNPHMTSSMARLLSDPLMSQEVEVSWLQPQPPRGGSRRLVRSPSMPERLDRPVLKRAPAPPDPGPDPCPLAKRWRSTAPIHEEEEEEGDGRKRGRLLKKTLSLSDVETRGEPGDLGLIGDFSKVRQSRCTPDSQPATLGVCARASSCACVCVSAFSCGYVCVAPTSAHLGSSVQQGSGFTSLGG</sequence>
</entry>
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                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:potassium:proton antiporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="KEA4/5/6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
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        <property type="entry name" value="POTASSIUM/PROTON ANTIPORTER-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16254:SF14">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5004868308" evidence="11">
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            <end position="676"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="476"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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        <location>
            <begin position="278"/>
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    </feature>
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        <location>
            <begin position="124"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="676" mass="75235" checksum="54CD770A66EE6D2E" modified="2014-04-16" version="1">MKGLCVVLLWLHLAGGLPGASREHGEVAKQAIKLHRGRGAPVTQGRRWVLENCRRLSGLMRQKSTVMEKLGAAIAAVERDRGLSGAEKLFQVHTFEIFQKELNESENSIFQAVHGFQRALQGDYKDVVNMKESSRQRLEALREAAIKEEQEYVELLAAEKHQAEALKIIQHQNKSMSMLDEILEDVRKAADRLEEEIEDHAFDYNKLMKGVNVEAVLRVEEDEEDSQRNASKREVEDDLGLSMLIDSQNNQYILTKPRDSTMPRADHHFIKDIVTVVMLSLPCGWLCTSIGLPTMFGYIMCGVLLGPSGLNSIKSVVQVETLGEFGVFFTLFVVGLEFSPERLRKVWKISLQGPCYMTSLMVAVGLLWGHVLKIRPTQTVFISTCLSLSSTPLVSRFVAGSARGDKEGEIEYSSVLLGMLVMQDVQLSLFIAVMPSLIQAGVENVDSIFMETLQILLLVGQVLFSLAAVLLVCVLTKTYVIGPFYRKLHAECKGNKEILVLGVSAFIFLMLSVTDFLDVSMELGCFLAGALFSSQGHLLTTEVMSCIEPIRDFLAIIFFASIGLHVFPTFVLYELTILLILTFSLVVMKFIIAVLVLSMILPQGSQHIKWIVSAGLAQVSEFSFVLGSRARRTGIISREVYLLILSVTTLSLLLAPVLWKAATLKCVPRAERWTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV30</accession>
    <name>W5MV30_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like family member 38b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01004698">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV30"/>
    <dbReference type="FunCoup" id="W5MV30">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012239"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012260.1">
        <property type="protein sequence ID" value="ENSLOCP00000012239.1"/>
        <property type="gene ID" value="ENSLOCG00000010012.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696585"/>
    <dbReference type="KEGG" id="loc:102696585"/>
    <dbReference type="CTD" id="559083"/>
    <dbReference type="eggNOG" id="KOG4441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157647"/>
    <dbReference type="HOGENOM" id="CLU_004253_14_6_1"/>
    <dbReference type="InParanoid" id="W5MV30"/>
    <dbReference type="OMA" id="EVALTCF"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010012">
        <property type="expression patterns" value="Expressed in muscle tissue and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18476">
        <property type="entry name" value="BACK_KLHL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030568">
        <property type="entry name" value="KLHL38_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="581" mass="66077" checksum="61A4F0666006850F" modified="2014-04-16" version="1">MENMIPEAFHFKDKEHPSNLLSQLDGLRQERILTDVLLCTGDREIPCHRIVLVSGSPYFRAMFCGDFKESQQAKISMRGIASDVLSSIIDYVYTGCIAITMELVLPLMQAASMLQYPRLFDACSAFLQEQLSPDNCLSMIRLSEILHCESLRAKAREVAVCSFSDVTASEDFRELSLSELEGYLGDDRLCAEEEQVFETLLAWIHHDPFSRKGAIHNLFQKVRLRHVHPTYLFQFIANDPLIQASPLCTDIIESVRRLMFSVSAKCARELRPQWAAPRRYPCQEMLVVVAGRKNNQQTSREALLYDDRTRRWQWLAKLPLRLYKASFACLHGILYVLGGLVVNGRDSAVSSSVYTFSLKSNQWRAADPMLKPRYAHQSVSYLNFIFVLGGIGPGRQVSSSVERYNSMFNLWEVVAPLPTAVLHPAVAAADQRIYVFGGEDVMQNPARLIQVYHISRNMWSRLETRMVKNVCAPAAVIEDKIYIIGGYTRRMVAYDIKTNKFVKCAQMRERRMHHGATVIDSKLYVTGGRYINNHNVIEDSDCFESYDPNTDLWTSKGTLPYKLFDHGSLPLICVTNKGDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5MV50</accession>
    <name>W5MV50_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytokine like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001194">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MV50"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012259"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012280.1">
        <property type="protein sequence ID" value="ENSLOCP00000012259.1"/>
        <property type="gene ID" value="ENSLOCG00000010022.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S2F0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016221"/>
    <dbReference type="InParanoid" id="W5MV50"/>
    <dbReference type="OMA" id="IMKFMET"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010022">
        <property type="expression patterns" value="Expressed in larva and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029253">
        <property type="entry name" value="CYTL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15974">
        <property type="entry name" value="CYTOKINE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15974:SF0">
        <property type="entry name" value="CYTOKINE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15153">
        <property type="entry name" value="CYTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868331" evidence="1">
        <location>
            <begin position="21"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="148" mass="17188" checksum="532F1932C28E71C7" modified="2014-04-16" version="1">MKPGVGTFIVLCSLIFLANCAPPPTCYSRVLSYSKEIMKFMETLQKSYRTKSCMEALPKMYLDVHNSCIMPKLRDFLYVLENLTRPECRERPRILGLKRKIRSLYIIINKVCYRDLVFYTDDCDALVTGESTPRQGGDQLPLYEKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWA7</accession>
    <name>W5MWA7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Carboxypeptidase Z</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001257">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001258">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWA7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012666"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012690.1">
        <property type="protein sequence ID" value="ENSLOCP00000012666.1"/>
        <property type="gene ID" value="ENSLOCG00000010330.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2649">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156391"/>
    <dbReference type="HOGENOM" id="CLU_006722_5_1_1"/>
    <dbReference type="InParanoid" id="W5MWA7"/>
    <dbReference type="OMA" id="DCMHAFE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010330">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000012">
        <property type="entry name" value="Carboxypeptidase Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000021">
        <property type="entry name" value="Carboxypeptidase Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000022">
        <property type="entry name" value="Carboxypeptidase Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF63">
        <property type="entry name" value="CARBOXYPEPTIDASE Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866672" evidence="13">
        <location>
            <begin position="38"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="14">
        <location>
            <begin position="57"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="15">
        <location>
            <begin position="206"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="12">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="683" mass="78177" checksum="B93FD4C584059993" modified="2014-04-16" version="1">RRVHPCECHITMHMGYKRIKMNSIVFALNLLASIVWSAPPRCEPGDAILGRCRIPVEEKPQCTNIVLGYCDDMPYSKTLFPNLLDNKNREELEMGTEYLLLSVIHTLLNGECTPDIRMLGCSIIAPRCENGKIMKPCRSTCESVRKNCIHAFEGIKMAWPYFLDCDRFFVGEEEGCYDPLENLREKQEVALASISPEEPSTIIQFTYHSHLQMENVLKKTAARCSDISRTYSIGRSFEGKDLLVIEFSDNPGQHELLEPEVKYIANMHGNEVLGRQLLIYLAQYLCSEYQLGNERIQTLINNTRIHLFPSMNPDGYELAAAEVEDTNEQEQDEGHRYNGWTSGRANAQNIDLNRNFPDLTSIVYSRRRNKGYRTDHVPIPDSYWFSKYVVAPETYAVMKWVRSIPFVMSASLHGGELVVSYPYDFSRHPEEEKMFSPSPDEQVFKLLSRIYADAHPTMSDNSTDRCGASFASKGGIINGAMWYSFSGGMSDFNYLHTNCFEITVELGCDKFPSEDELYLGWQQNKEPLLSYLEAVHRGIKGIVKDETGKGIKGARISVRGVRHDIFSAEDGDYWRLLTSGTHIITASAPGYTKVMKKINLPPRLQKAGRVDFILKREILEPGINDHFPAQDTYDRFDPFNQFERYTQRETGEPGEEEVQEKPWWWSYFALSGNAAPTWLLRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX60</accession>
    <name>W5MX60_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Glutathione S-transferase 3, mitochondrial</fullName>
            <ecNumber evidence="16">4.4.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Glutathione peroxidase MGST3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">LTC4 synthase MGST3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:48620"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57450"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:90632"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:48621"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>15-deoxy-Delta(12,14)-prostaglandin J2 + glutathione = 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathione</text>
            <dbReference type="Rhea" id="RHEA:75963"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:85236"/>
            <dbReference type="ChEBI" id="CHEBI:194498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:75964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>leukotriene C4 = leukotriene A4 + glutathione</text>
            <dbReference type="Rhea" id="RHEA:17617"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="EC" id="4.4.1.20"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:17619"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Lipid metabolism; arachidonate metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; leukotriene C4 biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.20" evidence="16"/>
    <dbReference type="EMBL" id="AHAT01000180">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01000181">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX60"/>
    <dbReference type="FunCoup" id="W5MX60">
        <property type="interactions" value="824"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012969"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012996.1">
        <property type="protein sequence ID" value="ENSLOCP00000012969.1"/>
        <property type="gene ID" value="ENSLOCG00000010594.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S4E5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008608"/>
    <dbReference type="HOGENOM" id="CLU_110291_1_0_1"/>
    <dbReference type="InParanoid" id="W5MX60"/>
    <dbReference type="OMA" id="VIFNCIQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010594">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004464">
        <property type="term" value="F:leukotriene-C4 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006691">
        <property type="term" value="P:leukotriene metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000004">
        <property type="entry name" value="Microsomal glutathione S-transferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050997">
        <property type="entry name" value="MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250:SF26">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="161" mass="17986" checksum="BF1DCCEC8D09A162" modified="2014-04-16" version="1">VITQTEQENKKFSFGFSMVVLSKEYGYVLLTGAASFVMVAHLAINVGRARKKYKVEYPQMYSDDPENGNIFNCIQRAHQNTLEVYPAFLFFLAIGGLHNPRLASALGMTWVISREVYAHGYSTGDPKKRARGSFGSIALLGLVGMTANYGRILLGWVRPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXG8</accession>
    <name>W5MXG8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclin G1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021406">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXG8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013077"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013105.1">
        <property type="protein sequence ID" value="ENSLOCP00000013077.1"/>
        <property type="gene ID" value="ENSLOCG00000010677.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154726"/>
    <dbReference type="HOGENOM" id="CLU_062642_0_0_1"/>
    <dbReference type="InParanoid" id="W5MXG8"/>
    <dbReference type="OMA" id="CFYIAIK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010677">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20583">
        <property type="entry name" value="CYCLIN_CCNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000006">
        <property type="entry name" value="Cyclin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="57"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="33957" checksum="1C809563BB7DA653" modified="2014-04-16" version="1">MMIDTVAAPETTFPVQLKMLLEQEARYQPKLCGLRIIESAQDNGLRMTAALRDFQVKELLSLTRFFGFSAESFSLAVNLLDRFLAQMKVQPKHLSCVGLCCFYIAVKVSEDEKNIPLANDLIRISQHRFTVSDMMRMEKIVLEKLHWKAKATTSLHFLRLYYSHVIEQMPAERKKMLNIERLEAQLKACYCCFSFSKVKPSLLALAVLALEIQEQSLHELTEEFQSLKQYSNIKEGDLICVREMVAKCLAEYSSTKCTKPDNHKLRWIVSGRTARQLKHSYYRIAHLPTIPEAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MXX9</accession>
    <name>W5MXX9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004634">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MXX9"/>
    <dbReference type="FunCoup" id="W5MXX9">
        <property type="interactions" value="1065"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013238"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013265.1">
        <property type="protein sequence ID" value="ENSLOCP00000013238.1"/>
        <property type="gene ID" value="ENSLOCG00000010805.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263525"/>
    <dbReference type="HOGENOM" id="CLU_168377_2_0_1"/>
    <dbReference type="InParanoid" id="W5MXX9"/>
    <dbReference type="OMA" id="CEEVMEY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010805">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="72" mass="8090" checksum="A3A249C8C14E7190" modified="2014-04-16" version="1">MPVINVDDLTDKDKLKMEVDQLKIEVKLERALVSKCCEEVRDYIEAGSAEDPLVKGIPEEKNPFKEKGGCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MY04</accession>
    <name>W5MY04_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Janus kinase and microtubule interacting protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JAKMIP family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01021427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631888.1">
        <property type="nucleotide sequence ID" value="XM_006631825.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MY04"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013291.1">
        <property type="protein sequence ID" value="ENSLOCP00000013263.1"/>
        <property type="gene ID" value="ENSLOCG00000010819.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684133"/>
    <dbReference type="KEGG" id="loc:102684133"/>
    <dbReference type="CTD" id="9832"/>
    <dbReference type="eggNOG" id="ENOG502QTTJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153713"/>
    <dbReference type="HOGENOM" id="CLU_020294_1_0_1"/>
    <dbReference type="InParanoid" id="W5MY04"/>
    <dbReference type="OMA" id="RNLELEX"/>
    <dbReference type="OrthoDB" id="6424487at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010819">
        <property type="expression patterns" value="Expressed in brain and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024836">
        <property type="entry name" value="JAKMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031994">
        <property type="entry name" value="JAKMIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935:SF7">
        <property type="entry name" value="JANUS KINASE AND MICROTUBULE-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16034">
        <property type="entry name" value="JAKMIP_CC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Janus kinase and microtubule-interacting protein C-terminal" evidence="5">
        <location>
            <begin position="409"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="472"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="711"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="822" mass="96578" checksum="24F4B64A0C961A7E" modified="2014-04-16" version="1">MAKKSRSKGEKPEALIAALQAANEDLRSKLTDIQIELHQEKCKVSKLEREKVQEGKKIREQEQHKHTVTLTELKAKMHEDKMKELQAVRENLLKQHEQELARTIKIKDAENQRLKTALNGLRDGSSDKVRTALTLEAKEEARKFFDSERLKLLQEIAELKATKKQTDEALSKMIQADKIKAGDLRSEHQLHQEQISKIKWDCERDIRRLVDEIKSKDRTIFALEKELETQTGFLQKLQLQKDALDEQLFLVKEAECNLGSPKREIPGRAGDGAEHCGSPDMRRNQRKMAELNSTIRKLEDRNTLLVDERNALLKRVRETEKQCKPLLEKNKLLTKRNDELTQSLQRLEEKLKVLTKENLEMKDKMSSHHPLKKLKSLNDLDQAHEEQEIEFLKLQVLEQQNMIDDLTRDREKLLRRKKHKRSSKPIKRHIVVDTFFGYDEESMDSETSSVASFRTDRTPATPDEDLDESLAAEESELRFRQLTREYQALQRAYALLQEQKGGILDAEKEAKAHEQLQAEVLRYKAKIEDLEKEISQKGQDSKWVEEKQLFLRRNQDLLDKVEKLEADINRLQQELQDSKDQNELLEFRILELEERERRSPPFNLQIHQPFSEGVSALQIYCIKEGVKDVCIPDLIKLLDILGDNGNLRNEEQVAIIQASTVLSLAEKWIQQIEGTEAALHQKMLDLELEMEMFCKQKGYLEEELDYRKQALDQAYMQIQELEATLYNALQQDTVIKYGESLTERQKDELRTAVEKLRRQMLRKSREYDCQILQERMELLHQAHQRIRDLEDKTDIQKRQIKDLEEKFLFLFLFFSLAFILWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYE2</accession>
    <name>W5MYE2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein lin-52 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lin-52 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_006632369.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYE2"/>
    <dbReference type="FunCoup" id="W5MYE2">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013401"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013430.1">
        <property type="protein sequence ID" value="ENSLOCP00000013401.1"/>
        <property type="gene ID" value="ENSLOCG00000010928.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691206"/>
    <dbReference type="KEGG" id="loc:102691206"/>
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    <dbReference type="eggNOG" id="KOG4402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008402"/>
    <dbReference type="HOGENOM" id="CLU_143062_1_0_1"/>
    <dbReference type="InParanoid" id="W5MYE2"/>
    <dbReference type="OMA" id="NVQNTAY"/>
    <dbReference type="OrthoDB" id="5834362at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
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        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018737">
        <property type="entry name" value="DREAM_LIN52"/>
    </dbReference>
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        <property type="entry name" value="LIN52 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31489:SF2">
        <property type="entry name" value="PROTEIN LIN-52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10044">
        <property type="entry name" value="LIN52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="131" mass="14652" checksum="DD954A6ED6491AFF" modified="2014-04-16" version="1">MNLSATANRPRWSRDVVLKMASPSGGDDLETSLLSFEKLDRASPDLWPEQLPGVAEFAASFKSPITNSPPKWMAELENDDIEMLKELGSLTTANLMEKVKGLQNLAYQLGLEESREMTRGKFLNILEKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZQ2</accession>
    <name>W5MZQ2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015200542.1">
        <property type="nucleotide sequence ID" value="XM_015345056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZQ2"/>
    <dbReference type="SMR" id="W5MZQ2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013861"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013890.1">
        <property type="protein sequence ID" value="ENSLOCP00000013861.1"/>
        <property type="gene ID" value="ENSLOCG00000011277.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691733"/>
    <dbReference type="KEGG" id="loc:102691733"/>
    <dbReference type="eggNOG" id="KOG1757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000140979"/>
    <dbReference type="HOGENOM" id="CLU_062828_2_2_1"/>
    <dbReference type="InParanoid" id="W5MZQ2"/>
    <dbReference type="OMA" id="PVGRIHT"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011277">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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            <end position="93"/>
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            <dbReference type="Pfam" id="PF16211"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="128" mass="13583" checksum="E031E537B2930371" modified="2014-04-16" version="1">MAGGKAGKDSGKTKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5N017</accession>
    <name>W5N017_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000011375.1"/>
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    <proteinExistence type="predicted"/>
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            <begin position="1"/>
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            <begin position="1"/>
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            <begin position="48"/>
            <end position="62"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="298" mass="32966" checksum="111AD0416EC20630" modified="2014-04-16" version="1">MQEKDSLADVRGRGSQQGEPPSSERRQASHSAAQEYKTPPEQPHHRKPAQDSTHKPSQDDKIKHSKVGCHSGSWPRGTAEDNRGILPGDICRRGLSDRLGPLECSKKTEKLASKGASARGDGRCQTSYHTQQDCAAGSWPKAARGCSQKAGLHSTQEGDLPKSKKGLPNPRVLRNQLLKARASRLADERGGVTTDEEMRSEVKMGRYWSKTERRRHLLTAREQRQRRLARGSGGEGARRKEASSVVLELSNRKRTQLRNRKLLDSWTTIEELLTHGTKTAVGTSEIMCPSPLLSVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5N022</accession>
    <name>W5N022_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">CASK interacting protein 2</fullName>
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    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
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            <taxon>Lepisosteus</taxon>
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    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01000278">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000011372.1"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158256"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011372">
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09497">
        <property type="entry name" value="SAM_caskin1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_caskin1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="caskin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000225">
        <property type="entry name" value="caskin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000062">
        <property type="entry name" value="caskin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000042">
        <property type="entry name" value="caskin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032232">
        <property type="entry name" value="Caskin1-CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035497">
        <property type="entry name" value="Caskin1/2_SAM_1"/>
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    <dbReference type="InterPro" id="IPR035498">
        <property type="entry name" value="Caskin1/2_SAM_2"/>
    </dbReference>
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        <property type="entry name" value="Caskin2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032117">
        <property type="entry name" value="Caskin_C"/>
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    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF18">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF16907">
        <property type="entry name" value="Caskin-Pro-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Caskin-tail"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="281"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="11">
        <location>
            <begin position="505"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="11">
        <location>
            <begin position="574"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="722"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="923"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="994"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1137"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="722"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1039"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1144"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1163"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1301" mass="139612" checksum="D2FB2863185AD377" modified="2014-04-16" version="1">MGKEQDLLQAVKNGDLPSAQKLLAKIKAGRTKLLGSTKRLNVNYQDSDGFSALHHAALTGTTDLISLLLEAQATVDIKDSNGMRPLHYAAWQGKADSVFMLLRAGGSVNMPSQDGQIPLHLAAQYGHYEVSEMLLQHQSNPCIVNKAKKTPLDLACEFGRLKVAQLLLSSNMSVALLEGERKDSSDSSFNTPLHLAARNGHKDIIRLLLKAGIDINKITKAGTALHEAALYGKTEVVRLLLDAGIDVNIRNTYNQTALDIVNQFTTSHASKDIKQLLRDASGVLQVRALKDYWNLHDPTALNIRAGDIIMVLEQHMDGRWKGHIHDSPRGTDRVGYFPPSIVEVISRRSAGGTLSRHSSLPTHRQQFLSRPPPASLTSPQTDDSYTLYTAPPHLAFTRTGGLDDSTAGDRNSVGSTGSVGSTRSAGSGQSTDSNNGQAPGGLADGAKPAPSVGDQVQQISAGSDMAKQGEQPSGGLRRQSQNSLRSGEQSFSSLFIRPEQLLEGKDAEAIYQWLSEFQLEQYTANFLNAGYDVPTISRMTPEDLTAIGVTKPGHRKKISIEISNLNIPEWLPDYIPSDLGEWLSAIGLPQYHKKLSENGYDSISIVRDITWEDLQEIGITKLGHQKKMMLAVKKLCDIQKAQNLAEAGSRTLRRKLPGALELVTIEPLEGGDCPSPHTQKMLTFQDSELSAELQSAMSNTYSGCQDGLVIKSVAGISCSQESIGTRSRGSGHSQEHPLARSAPSSRSQESLGSGDSSSGSSGGRSRTNQDTFRLVGVPSKMALLQQNPTSITPPHTPGKVPGGFVYPPVPVKAKPNASPVLPFQGSPQGSPAQKAFTYLHSHCGSTDLDRSPFTKPLAGAVPLLSQRGAPLNGDLFKPKKRSQSLTRYALSDGEPDEEEMAPTGNLASYATLTRRPGRSQLVHLQAGPERNVNRSQSFAIRARRKGPPPPPPKRLSSVSGVVTSELADAMTPGGVETESVGSVRTITAMLEVSDPASPAEPGSLPLPKPALPQATREHVGSRRRTLSEPGVSETDKVDISSSPHNSSSECIPFAEEGNLTIKQRPKTGGPLKPESTAEAAEKARAPKTLEVPEFNLKESDTVKRRHKPKEQQHQPPTESSDVGFRIAEIEKSLQCLEKGTPGKPQKPPLSPKPASPQKPVADSSYTRHSTLATSEAGTHAVTLNVVQSLAFAAPTACSPRSPVQTPTSSLQPHMVPQSQQLKAGKPQTCLLGPAPGSVLVQRRLEQTSTSLEAALKAVERKLTLEDNTDGGTSTVRSAGNILDDIGNMFDDLADQLDAMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N059</accession>
    <name>W5N059_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">T cell receptor gamma variable 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017679">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N059"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014018"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014047.1">
        <property type="protein sequence ID" value="ENSLOCP00000014018.1"/>
        <property type="gene ID" value="ENSLOCG00000011394.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SDDP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153143"/>
    <dbReference type="HOGENOM" id="CLU_077975_7_0_1"/>
    <dbReference type="InParanoid" id="W5N059"/>
    <dbReference type="OMA" id="CISCIAT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011394">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001649">
        <property type="entry name" value="T cell receptor gamma, variable 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-1279">T cell receptor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="123" mass="13715" checksum="0CF09304742DE0CE" modified="2014-04-16" version="1">MADYEVVSTTLLCTLSETAITLKQDQVTLTKGTTKTARISCKVNAENFGREYIHWYQQKEGEALKRILYIAGAGNPTIEEEYKKAGFSADKITAKEESNLFIASVSDSHSATYYCACWDTHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0F9</accession>
    <name>W5N0F9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nipsnap homolog 3A (C. elegans)</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0F9"/>
    <dbReference type="FunCoup" id="W5N0F9">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014118"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014147.1">
        <property type="protein sequence ID" value="ENSLOCP00000014118.1"/>
        <property type="gene ID" value="ENSLOCG00000011488.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2883">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183018"/>
    <dbReference type="InParanoid" id="W5N0F9"/>
    <dbReference type="OMA" id="WYESADH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011488">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000017">
        <property type="entry name" value="Protein NipSnap homolog 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000019">
        <property type="entry name" value="Protein NipSnap homolog 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF19">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="57"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="165"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="266" mass="30322" checksum="E20F323659AF61DE" modified="2014-04-16" version="1">MFSVRNAIRKAFTLPDKIKLKITPKVCGVLRTELIRTREKLHASLSTGPQQQNGTFYELRTYCIKPEHNSAFLKLTNEKIQLRTAHSELLGYWSVEYGGLNEVFHIWKYESYAQRAAVRAALAQDPEWQNQYISKALPLLSSQDSEVTYLVPWCEIDKPSKEGVYELVTFQMKPGGPAVWGKAFRAAVSTHAKAGYAHLVGVFHSEFGLLNRVHALWWYEDADRRAATRRVAHQDARVVAAVRESVSYLDSQRNKLLIPMAFSPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N114</accession>
    <name>W5N114_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-adducin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Erythrocyte adducin subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N114"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014323"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014352.1">
        <property type="protein sequence ID" value="ENSLOCP00000014323.1"/>
        <property type="gene ID" value="ENSLOCG00000011644.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158581"/>
    <dbReference type="InParanoid" id="W5N114"/>
    <dbReference type="OMA" id="LEWESWM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011644">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903393">
        <property type="term" value="P:positive regulation of adherens junction organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903142">
        <property type="term" value="P:positive regulation of establishment of endothelial barrier"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000002">
        <property type="entry name" value="alpha-adducin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005451">
        <property type="entry name" value="PRK07044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF4">
        <property type="entry name" value="ALPHA-ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="17">
        <location>
            <begin position="147"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="417"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="573"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="658"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="593"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="772"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="794" mass="87485" checksum="079950CF9DC9689B" modified="2014-04-16" version="1">MNGDSGAGVVTSPPPTTAPHKERYFDRVDESSPEYQRERNMAPDLRQDFNMMEQRKRVSMILQSPAFCEELETMIQDQLKKGKNPTSLLALQQIADFMNTSMPSIYPATPQGGMAALNMSLGMVTPVNDLRGSDSIAYEKGEKLLRCKLAAFYRLTDLFGWSQLIYNHLTVRLNSEQERFLIVPFGLLYSEVTASNLVKINLQGEIVDRGSTNLGVNQAGFTLHSAIYSARPDIKCVVHIHTPAGAAVSAMKCGLLPISPEALALGEVAYHDYHGILVNEEENVLIQKNLGPKSKVLILRNHGLVSVGETVEEAFYYIHNLVRACEIQVRTLASAGGPDNLVMLDPGKYKAKPHFSESASDTNASHPKWQVGEQEFEALMRMLDNLGYRTGYPYRCPSLRDKTKKYSDVEIPPSAMGYSFAEDSDSGARSPLKHSFQRQQREKTRWLNSGRGDDTSEEGPNDGSPKSKTKWTKEEGLRQAVAANQFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLTGAVVDRSYVQGELVTASKAIIEKEYQPRVIMSTTGPNPFNKLTDQELEEYRKEVERKQKGPEVQGEEAQATECTSQQELPSSNLPVGSEDPGLEGLALGPGAAKTPAVEEMDSVESPHREFHFAVLRALSSNTDLLNEASDQSQQEAVEEVKDQSPAVCTPTRTPPSTPIRAEEESQQDQTYKDESDAATLRQTLPDLTPDEPSEAPAFPPASVGESAAAQPESEPQSQADDPAAASADLGSDESPSKSPSKKKKKFRTPSFLKKNKKKSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1T1</accession>
    <name>W5N1T1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PC4 and SRSF1 interacting protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HDGF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1T1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014590"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014619.1">
        <property type="protein sequence ID" value="ENSLOCP00000014590.1"/>
        <property type="gene ID" value="ENSLOCG00000011863.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154706"/>
    <dbReference type="InParanoid" id="W5N1T1"/>
    <dbReference type="OMA" id="HKKFFAG"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011863">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20151">
        <property type="entry name" value="PWWP_PSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000017">
        <property type="entry name" value="hepatoma-derived growth factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036218">
        <property type="entry name" value="HIVI-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021567">
        <property type="entry name" value="LEDGF_IBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF42">
        <property type="entry name" value="PC4 AND SFRS1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11467">
        <property type="entry name" value="LEDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140576">
        <property type="entry name" value="HIV integrase-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PWWP" evidence="9">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="457"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="519" mass="58879" checksum="5A6925C8A5397CB3" modified="2014-04-16" version="1">MTRDFKPGDLIFAKMKGYPHWPARIDEVPDGAVKPSNAKLPIFFFGTHETAFLGPKDIFPYLPNKEKYGKPNKRKGFNEGLWEIENNPKVELSGQKVPAKRARLAVADAEGEQEETEGVMEDSELLPESADDTAQKGTPETPVKAKRGRKKKSEVEQDSEKEEIPTSPTSSPPEKVLIFVCPPVGSEVPTPKRRGRKPKQSRPYPSCSWDSSEADKKRKRGPEEKPAKSLKPEEEEKKTDGRKRKEEEKSKEANGKKEPEAKRRKNAKGETSSESEEDEEKINEQKNKNDRFSAARRRNLLKSQQDSLEMDRDERRRKQEDQKATDLVRQGKDEGKKLDEKKMDKKKDLSTESRLQRLHGEIKISLKIDSPDVKKCIEALDELGTLQVTTQHLQKHSELIATLKKIRRFKASQDIMDKATMLYNKFKTMFLVGEGDSVISQVLNKSLAEQRQYEEAKKGGLRKAEVKEQTTESKTVNGDANLGESSPTQQEQDKEKLTEDPGLVEQSNTAKVQPQEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N287</accession>
    <name>W5N287_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Intraflagellar transport protein 56</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tetratricopeptide repeat protein 26</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT56 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01036115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036116">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036119">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036122">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036124">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N287"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014746"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014775.1">
        <property type="protein sequence ID" value="ENSLOCP00000014746.1"/>
        <property type="gene ID" value="ENSLOCG00000011992.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3785">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000159"/>
    <dbReference type="HOGENOM" id="CLU_2066760_0_0_1"/>
    <dbReference type="InParanoid" id="W5N287"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011992">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030511">
        <property type="entry name" value="TTC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="119" mass="13844" checksum="D06F8C1888A34FAA" modified="2014-04-16" version="1">MEEYRSLTQRDGCSPDVWVNLACTYFFLGMYAEAEEAALKAPKSQLQNRLLFHLAHKFNDEKKLMGFHQNLQDVTEDQLSLASIHYMRSHYQEAIDIYKRILLENREFLIIPSMVSGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3A2</accession>
    <name>W5N3A2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lymphoid enhancer-binding factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01017715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017716">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3A2"/>
    <dbReference type="FunCoup" id="W5N3A2">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015111"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015140.1">
        <property type="protein sequence ID" value="ENSLOCP00000015111.1"/>
        <property type="gene ID" value="ENSLOCG00000012276.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159660"/>
    <dbReference type="HOGENOM" id="CLU_013229_5_0_1"/>
    <dbReference type="InParanoid" id="W5N3A2"/>
    <dbReference type="OMA" id="NFSTCKA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012276">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033336">
        <property type="term" value="P:caudal fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021879">
        <property type="term" value="P:forebrain neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021979">
        <property type="term" value="P:hypothalamus cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055001">
        <property type="term" value="P:muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048922">
        <property type="term" value="P:posterior lateral line neuromast deposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060784">
        <property type="term" value="P:regulation of cell proliferation involved in tissue homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900052">
        <property type="term" value="P:regulation of retinoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000004">
        <property type="entry name" value="lymphoid enhancer-binding factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF11">
        <property type="entry name" value="LYMPHOID ENHANCER-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="297"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="13">
        <location>
            <begin position="297"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="268"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="397" mass="44401" checksum="F0EDB65493A2AF9E" modified="2014-04-16" version="1">MPQLAGGGGGGGDPELCANDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNETEINPNSNSHEAARQSQVPQDSYHEKHREHSDDAGKQDGSLYNKGHPYQGYPGYIMMSNMNNDSYLSNGSLSPPMPRTSNKVPVVQPSHAVHPLTPLITYSDEHFAPGPHSGHHPQDVNSKQGMPRHHPGPDIANFYPLSPGGVGQITPPLGWQGQPVYPISGGFREPYPSALPVDTPMARFSHHMVPGPPGPHTTGIPHPAIVNPQVKHEHPHDSDLMHMKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQEPASGTSQRMKTAYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4C6</accession>
    <name>W5N4C6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MafG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">V-maf musculoaponeurotic fibrosarcoma oncogene homolog G</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000349">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4C6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015485"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015513.1">
        <property type="protein sequence ID" value="ENSLOCP00000015485.1"/>
        <property type="gene ID" value="ENSLOCG00000012579.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160070"/>
    <dbReference type="HOGENOM" id="CLU_112948_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4C6"/>
    <dbReference type="OMA" id="QHSRYRF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012579">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045604">
        <property type="term" value="P:regulation of epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="14">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="171" mass="18855" checksum="C94E60151BF7907C" modified="2014-04-16" version="1">MTTANKGSKALKVKREPGENGTSLTDEELVTMSVRELNQHLRGLSKEEILQLKQRRRTLKNRGYAASCRVKRVTQKEELEKQKAELQQEVEKLASENASMKVELDALRSKYEALQNFARTVARSPVAPARGPIASVIGPLIPGKVATTSVITIVNPQTEHLPSPSPPHLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4H2</accession>
    <name>W5N4H2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DnaJ homolog subfamily C member 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a co-chaperone regulating the molecular chaperones HSP70 and HSP90 in folding of steroid receptors, such as the glucocorticoid receptor and the progesterone receptor. Proposed to act as a recycling chaperone by facilitating the return of chaperone substrates to early stages of chaperoning if further folding is required. In vitro, induces ATP-independent dissociation of HSP90 but not of HSP70 from the chaperone-substrate complexes. Recruits NR1I3 to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4H2"/>
    <dbReference type="FunCoup" id="W5N4H2">
        <property type="interactions" value="1196"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015531"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015560.1">
        <property type="protein sequence ID" value="ENSLOCP00000015531.1"/>
        <property type="gene ID" value="ENSLOCG00000012614.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155338"/>
    <dbReference type="HOGENOM" id="CLU_015935_3_1_1"/>
    <dbReference type="InParanoid" id="W5N4H2"/>
    <dbReference type="OMA" id="KMCLGLD"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012614">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNAJ PROTEIN P58IPK HOMOLOG"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00028">
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        <property type="match status" value="8"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNAJ_2"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
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        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
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        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="14">
        <location>
            <begin position="404"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053178"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
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    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="519" mass="59185" checksum="9942835DE9E88060" modified="2014-04-16" version="1">RCLRLFMSACSGRAGSTLSLPKSNMDAVDCDVIMDPEPQIMNEEELAREAECFKEQGNAFYIKKDYTEAFNYYTKAIADACPKNASYYGNRAATLMMLSRFREALEDAQQAVRLDDSFVKGHLREGKCHLSLGNAMAARRCFQKVLELEPDNSQAQQELKNADAVLEYEKMAEFDFEKRDFRKVVFCMDRALEYAPVCHRFKILKAECLALLGRYPEAQSVASDILRLDSTNGDALYVRGLCLYYEDCIDKAVQFFVQALRMAPDHEKARLACRNAKALKAKKEEGNKAFKDGNYEVAYELYSEALQIDPNNIKTNAKLYCNRGTVGSKLNKIEQSIEDCTNAIKLDETYIKAYLRRAQCYMDTEQYEEAVRDYEKVYQTEKTKEHKILLKTAQLELKKSKRKDYYKVLGVDKNATEDEIKKAYRKRALMHHPGSDRHSGASPEVQKEEEKKFKEVGEAFTVLSDPKKKARYDSGQDLDDDGMNMGDFDANNIFKAFFGGPGGFSFEASGPGNFFFQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4N7</accession>
    <name>W5N4N7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TBP-associated factor 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016576">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4N7"/>
    <dbReference type="FunCoup" id="W5N4N7">
        <property type="interactions" value="1086"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015596"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015625.1">
        <property type="protein sequence ID" value="ENSLOCP00000015596.1"/>
        <property type="gene ID" value="ENSLOCG00000012673.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017567"/>
    <dbReference type="HOGENOM" id="CLU_070829_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4N7"/>
    <dbReference type="OMA" id="SAHNYCE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012673">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22918">
        <property type="entry name" value="HFD_TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="transcription initiation factor TFIID subunit 8 isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTP"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="13">
        <location>
            <begin position="31"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="312" mass="34313" checksum="1CFCEDAC4C23390C" modified="2014-04-16" version="1">MADPNVVGSGSLGSVTRSSGGKTSTSPAENYYLARRRTLQVVVSSLLTESGFEAAEKAAVESLTEMIQSYISEVGRCARAYCEHTARTQPTLSDVVVTLVEMGFNVDTLPIYAKRSQRMVITAPPVTNPPVIPKALTAGQKRLHPSHIPGYFPDFPDPHTYIKTPTFREPVSDYQVVREKAASQRRDVERALTRFMAKTGETQSLFKDDISTFPLIAARPSSIPYLNALLPSELELQQLEETDSSEQDEQTDNENASVNISNDESGADKENSVLPQGGALPTVKASEENVIDNPYLRPVKKPKVRRKNFNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5C5</accession>
    <name>W5N5C5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01023185">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000015864.1">
        <property type="protein sequence ID" value="ENSLOCP00000015834.1"/>
        <property type="gene ID" value="ENSLOCG00000012863.1"/>
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    <dbReference type="HOGENOM" id="CLU_3001719_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012863">
        <property type="expression patterns" value="Expressed in zone of skin and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867051" evidence="1">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="57" mass="6418" checksum="3E134B84E9579947" modified="2014-04-16" version="1">AQVIVVAVLSVGVLLQRSANSTEREEKPVKKKTKTIKLRKDYTCYLMVLSTSHEHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6E7</accession>
    <name>W5N6E7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM227B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
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    <dbReference type="EMBL" id="AHAT01026091">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="W5N6E7"/>
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        <property type="component" value="Linkage group LG3"/>
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        <property type="expression patterns" value="Expressed in testis and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029417">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560:SF2">
        <property type="entry name" value="PROTEIN FAM227B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14922">
        <property type="entry name" value="FWWh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="50090" checksum="FFE25C0EDAB110C8" modified="2014-04-16" version="1">MVSMEEPARNFQDFIRFQQLVDWPIMLEEKFNLTEQLSGYHSANEIAECLQKEAPFHKGIITELEQRVEELSCLLETHASKMLYSETPTEKTDVQPSDISPQILGLLKKARQKLRRKSAGVSPRIMNIKDCKYPGFTQNKYNDLPGQLDVLLLMDYISEAQNFNSGFLKVWKPFFLTDSSVAVLKDTFWWFYVHAFKPNPEDENKLFDRISENFVVLLMTVQPEVKDKLFKVYADCLAQAVYSAFHGAFPESHGHFDEDFKTELTDLISHWISGVKPVLYSWKKWNLNGLSEVLNGAGEVGKEPKILSVTSGTQCQMAFNLEELIKNKRKISVVQTVPFAAKRESGATTDVKTGVFARKWRESHSIGPNPESEHVPFMPGGQSPLVARYLQLHGIPHTVGGQHVKRTEIRKLPYPSKSSTILLKNVYATQTTNTKKSKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7P1</accession>
    <name>W5N7P1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkey-225f5.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01030996">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7P1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016650"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016680.1">
        <property type="protein sequence ID" value="ENSLOCP00000016650.1"/>
        <property type="gene ID" value="ENSLOCG00000013506.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S6PG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_065380_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7P1"/>
    <dbReference type="OMA" id="AIDWCPA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013506">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Zwint-1"/>
    </dbReference>
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        <property type="entry name" value="ZW10 INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31504">
        <property type="entry name" value="ZW10 INTERACTOR ZWINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="299" mass="34015" checksum="A20ECF46467066B4" modified="2014-04-16" version="1">RDQWKALKSQYKDEIQEVESLITVFKEKVDEVLARKEALCQLLHTLEQKKEECKEKQRIKAGKQQKARERAERVCARVQELEAALERGRHGLQLSGQRVSELQAQLSGAQQSLDTWSRAHSRLQLELQRLDGLSGVRVLSVRERELHVELNPRLLCPSLDLLPLSLSLRWTSDDLFTLQEDLEEQPVFHTPGRPLQDARSALLEVMQLYVEQGSLLAEIQRLHSRFAIDWRPAERKLVFLKTASIVCTLSVEEGYPTSGRVQLVSVQGGAQSLNIAGLQPPLGKPSLTEWLEFLTCCPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7P3</accession>
    <name>W5N7P3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Spectrin beta chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Composed of nonhomologous chains, alpha and beta, which aggregate to form dimers, tetramers, and higher polymers. Interacts with BCAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000016652"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016682.1">
        <property type="protein sequence ID" value="ENSLOCP00000016652.1"/>
        <property type="gene ID" value="ENSLOCG00000013491.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158908"/>
    <dbReference type="HOGENOM" id="CLU_000146_1_2_1"/>
    <dbReference type="InParanoid" id="W5N7P3"/>
    <dbReference type="OMA" id="EPADMTS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013491">
        <property type="expression patterns" value="Expressed in muscle tissue and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008091">
        <property type="term" value="C:spectrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014731">
        <property type="term" value="C:spectrin-associated cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21246">
        <property type="entry name" value="CH_SPTB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000018">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000019">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000028">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000033">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000049">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000059">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000083">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000130">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000214">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000338">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016343">
        <property type="entry name" value="Spectrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002297">
        <property type="entry name" value="Spectrin_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 13" id="KW-0117">Actin capping</keyword>
    <keyword evidence="9 13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="58"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="177"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="2191"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2103"/>
            <end position="2187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2298"/>
            <end position="2346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="896"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1420"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2103"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2123"/>
            <end position="2139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2330"/>
            <end position="2339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2346" mass="272182" checksum="0649EEE15FA3CFFD" modified="2014-04-16" version="1">VRASMTSTTDFDNVEIQQQYTRINTRFELGDEELDNDNSSARVFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETQDSRETRSAKDALLLWCQMKTAGYPNINITNFTSCWKDGMAFNALIHRHRPDLVDFNKLQRSNPTHNLQHAFQVAEQKLGITKLLDPEDVFTENPDEKSIITYVVAFYHYFSRMKALAVEGRRVGRVLDHAIETEKMIDKYESLASDLLNWIKQTIVILNSRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPREGALVSDINKAWENLEKAEHERERILRNELIRQEKLEQLARRFDRKAAMRETWLVENQRLVAQDNFGYDLPAVEAAKKKHEAIETDIAAYEERVQALVAVSEELEAEKYHDAKRINARKDNILRLWEYLLELLSARRGRLETNLTLQKTFQEMLHIIGWMDEMKTRLLSPDFGKHLLEVEDLLQKHALVEADIAVQADRVRSANATSLQFAEGDGYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFFWEMAEIESWIKEKEQIFSSLDYGKDLTSVLILQSKHSAFEDELAARRAHLQQTVTEGERMVQAKHFGAPKVKQRTADVVQQWEQLEDLAAFRRRNLQDTQRYFQFQGDADDLKAWLLDARRLMSSDDMGHDEYSTELLLKKHRDLHDEALKNGATLETLSKQADSLPEELRNTPDIQRRLNDIRDLYVDLLSRSELRKKKLQDALALYTIFSDTDACELWMNQKETWLVGLKTPKKLEDLEVVQNRRRLEILGQEMDNVESRVENVNKAAKQLEDSRHPSMREVKDCQNHLNRRWAAFKKMVEEKKRNVDFALSLHNYGLECDETQTWIQEKTRVIESTQYLGNDLAAVMTIQRKLYGMERDLAAIQDKLNFLKGEAQRLAAEHPENAKDILGRERELDRAWEELRQTLKDRGESLGEVSKLQTFLQDLDDFQAWLFKTQKGIASEEMPNSLPEAEQLLSLHDAIRDDMNQHEEDYHRVKDTGAVVTEGETDPQYQQLEQRLKGLDTGWDELRRMWASRKNFLGQGLDFQQFLRDAKQAESILNSQEYTLAHSERPDTLDGAETALRKHEDFMNTMAANEEKIADAVDSGQRLVDSGNIYSERVKDKIQSIKDRYKSNQRKAKEVSAKLKDNRDLQHFLQNTQDLMLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKGWLNKIDQEGEELMRAKPEFQPIVTERLEKLHRLWDELETTTQDKAKTLFDANRSELFDQSYADLAKWLQETTLQLQADGDIKDLTSANILLKKQQVMENQVQEHQKELEELQAQVQVHAPLGKERPEMETKHKTLEQKFQQLLTPLQERKEKLLAAKAVHQFYRDLADEILWVEERLPLAEATNHGKNLQAVQLLTKKNQTLQREIEGHQPRVDEVLNRGDRMATSENPESEKIRQQMKELQEIWVKLQEETAKRQGRLRGAQEAQQYYNDAAEAEAWIGEQELYMIADETAKNEQSAVVMLKRHLIQQQAVENYANSVKNLADRAQKMLAEGHPEGEQVIRCQGQVDKQYASLKELAEERRKKLDHTYHLFQLSREVEDLEQWIAERDLMASSQEMGQDIDHVTLLREKFREFARETGTIGQERVDNVNLLIDELIDSGHSEAATMAEWKDNVNESWGDLLELIDTRTQLLNSSYDLHKYFYDGKELVALIQEKHTQLPMDLGGDVSTAESFHRMHAAFERDIHSLGKQVKQFQETAARLHAQYAGDQAAAIQATEHEVVEAWKALLDACDGRRSQLVNTADKFRFFSLVRDLMAWMESIIQQIETQEKPRDVSSVELLMKYHQGIKSEIETRNPKFTACIELGKALLARRHQESEEIKEKLIQLTEKNREMMGKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYMASRDLGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKKQEQIRQMRQEEEKRIKEEMRRREMGDTADTLQLYTPEEQSLTGSAAEQRPAPLDETAAPDRPETSSGKRPSLPGPLPPPVESERAATLPSETQSVQPALLEGMLVRKHSLEGPGKRASNRSWNSLYCVLKPGELSCYKDAKSFYLGVTYHGEDPLSLSDAVCEILTNYKKKKHVFKLRLGDSSEYLFQCKDEEELQNWTQALESAAAPQGESQSSYGTRARSLPPLSSSTAGDTGSAKKDKEKRFSLFTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5N900</accession>
    <name>W5N900_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Zinc finger and BTB domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029672">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N900"/>
    <dbReference type="FunCoup" id="W5N900">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017109"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017139.1">
        <property type="protein sequence ID" value="ENSLOCP00000017109.1"/>
        <property type="gene ID" value="ENSLOCG00000013872.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161268"/>
    <dbReference type="HOGENOM" id="CLU_007011_1_1_1"/>
    <dbReference type="InParanoid" id="W5N900"/>
    <dbReference type="OMA" id="KMHYKRS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013872">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060030">
        <property type="term" value="P:dorsal convergence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060031">
        <property type="term" value="P:mediolateral intercalation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003307">
        <property type="entry name" value="KAISO-like zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000235">
        <property type="entry name" value="Zinc finger and BTB domain containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000437">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="168"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="668"/>
            <end position="695"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="696"/>
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        </location>
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        <location>
            <begin position="722"/>
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        </location>
    </feature>
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        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="402"/>
            <end position="474"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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        <location>
            <begin position="800"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="907"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1028"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
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        <location>
            <begin position="402"/>
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        <location>
            <begin position="437"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="533"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
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        </location>
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        <location>
            <begin position="558"/>
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        </location>
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        <location>
            <begin position="855"/>
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        </location>
    </feature>
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        <location>
            <begin position="875"/>
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        </location>
    </feature>
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        <location>
            <begin position="907"/>
            <end position="926"/>
        </location>
    </feature>
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        <location>
            <begin position="927"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1028"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017109.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1252" mass="138776" checksum="93F9205E14DD36D4" modified="2014-04-16" version="1">CGLCHRSFSLSSSLSVHMRLHRGGRTLSCRHCGKAFIHNKRLQSHEALCGQPRQQEQTALVAPAKEEDQGNCEDEEKEEEEEVGERREKEEEQEPQGRSAPARIHKKGRGFLGRHRSFQRTDLLAEEDHFVKVVDGHIIYFCTVCERSYMTLSSLKRHSNVHSWRRKYPCRYCDKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQKSFHGINPGLITSEKTPNGGYKQKVNALKLYRLLPMRSQKRPYKTYSQSLSENILLSSDNIPSVPLALPMDGGLPAPLGSDQLQSYINEKHTMGLQADPADYLLAPPLDPGGLGRGETTQPSAPTLPVHGINREGEAEEDETNLRVPGHAKGFCGTETSASTVIAYGHPKPSVIVHGTSVSSVIMHSNQVPSSSSKENEPASEQPSPKVARPIKKQVLREYIQSQKRASKSENSTAEEEGRQPSSADEEKRPQKTCKAHGKSVTYVAKPACVGGSSETKGGAPLCQITVRIGEEAIVKRSISETDLMRDKSPPPSKTKKADSPIEEPIHHHQQHHHHHRHRSKDNNEKMPKPCKAREYYFRREVREEESDQDAEDNLWRPYYSYKPKRKAIHVQKVKKSSWQRKLHYKRSLRLTKRAERLVQQSAQDEGHERQPDASSALEDEQGEPEEQEELWCQICNQSFQDYLSMKRHERHHQVGKAFECSTCGRHFSTVKKLDKHELTHLMEFVCLLCQETFVSRSLLEEHQGTHHTKTENTLPQAEVGAELSELEKSSLPRVGRRPSVRHSCPFCSKVCKTAAALGRHMKRHGSDASDVAETATPMVSEATASDPEVDSEANSLKLGVGTDTALPTQTTELSCGETREGQDKNLDMEASVPSPHCSKTVQPDQSLKSNRSDAPSGCAQSVLVLNGKDSPELHKLDTADSYKTERSPEAQTNAADHTTSTEDCLGLLPTIRTPGKSAATAPGTTQSNAGQDLSDKTAEQRVAALPREATPVQELTASSMISKKEEIPASVAVAVSPEFPVIQTTAKASSNAVPSREQTAPLVTSGNSAQRRLKSPTNPPVQDLLRPRMGPSPATQAMVHEGQGLIMSRLTGVSAPQSVLVSHKSEEEPPVPRDFHREVAYPVQEFPLPLIVPGGCRSSKKQEDNILVSYPASAIQFGPLGKMSNGDLGKLPFYPDPYQLLYGPQLLAYPYNLAALPMALNMMAPGDKGEPLPFLPTLFNYVNPCPGTVPQHHLVASSSQCSSSSSNSSNREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5N939</accession>
    <name>W5N939_LEPOC</name>
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        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01007011">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N939"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017148"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017179.1">
        <property type="protein sequence ID" value="ENSLOCP00000017148.1"/>
        <property type="gene ID" value="ENSLOCG00000013911.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SDY1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000072363"/>
    <dbReference type="HOGENOM" id="CLU_066149_0_0_1"/>
    <dbReference type="InParanoid" id="W5N939"/>
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        <property type="component" value="Linkage group LG7"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0015074">
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        <property type="term" value="P:DNA recombination"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_brk_join_enz"/>
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        <property type="entry name" value="Integrase-like_cat_sf"/>
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        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
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    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047118085" description="Tyr recombinase domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28529" checksum="09BC6C0E0A67EEEB" modified="2025-10-08" version="2">MCGIHFFHKLLFGMTCPFLIHPQITLLLKDFQWLEKPLPNQRAPLTSDILESCIYVLRSGYHSPWVDRTLESLFMLAFFGFLRCSEITASSTSFDPSSQPCLSDISILSSDTLTFYLKKSKTDQLGSGTLIYYFKLWSHISPYEPLSHYAHLRTSAGALPTDPLLVNEKGRIVTGSSHLQDVLLTAGYPPEQFSGHSFRSGAASSAARQGIPEYLIKQLSRWSSEAYRAYIKTNPSIIKQPQKSAKNSKNTKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9A5</accession>
    <name>W5N9A5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RASD family member 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01000591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9A5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017214"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017245.1">
        <property type="protein sequence ID" value="ENSLOCP00000017214.1"/>
        <property type="gene ID" value="ENSLOCG00000013963.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693440"/>
    <dbReference type="KEGG" id="loc:102693440"/>
    <dbReference type="CTD" id="565976"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165021"/>
    <dbReference type="HOGENOM" id="CLU_041217_9_3_1"/>
    <dbReference type="InParanoid" id="W5N9A5"/>
    <dbReference type="OMA" id="SPSRHQI"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013963">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF2">
        <property type="entry name" value="GTP-BINDING PROTEIN RHES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="410" mass="45345" checksum="DCDB22CB39CE2D5A" modified="2014-04-16" version="1">MDFLTAERARFPTAGRAEALRSCVDRTGNDPPGVRLPLLAGGQAAVLLGNTGTCGGAAERRLDYKNSTQHPAAPGFKVPGNLGTISKTGMGIIKTVTSQWRHQERKARVVRSASTGNRYPSSDPFPCKRSPYGQLAAAALRAQLFPKLSEPDPRLELLRSVKPHNCRRIVVLGAPRVGKTSIVRRFLREGFEERYVPTAEDFHRKLYQIRGETYQLDILDASGERGFPAKRRLSILTGDIFLLVFSVDDRGSLEEVRSLAVEIVEAKTKLLKSKESVCVPTVICGNKIDLEPHRRADTRAEVCRALRGRAFFETSAKDGTNLEEMFRALAERGGLPTETGPSQHRKISIRSYQALRSARRGGKENRAGEAPYGAVYPLARRPSFSTDLRQVLGPSPIRKRSKTLEKCNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9D2</accession>
    <name>W5N9D2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho GTPase-activating protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Rho-type GTPase-activating protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">START domain-containing protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">START domain-containing protein 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">StAR-related lipid transfer protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">StAR-related lipid transfer protein 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with TAX1BP1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration. Interacts with tensin TNS1 (via N-terminus); the interaction is decreased by phosphorylation of TNS1. Interacts with TNS3 and PTEN; in resting cells, interacts with TNS3 (via C2 tensin-type domain) but, following growth factor stimulation, TNS3 and PTEN are phosphorylated which leads to weakened interaction with TNS3 and enhanced interaction with PTEN. Interacts (via C-terminus) with tensin TNS4 (via SH2 domain); the interaction is independent of tyrosine phosphorylation of DLC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002205">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9D2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017241"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017272.1">
        <property type="protein sequence ID" value="ENSLOCP00000017241.1"/>
        <property type="gene ID" value="ENSLOCG00000013982.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183061"/>
    <dbReference type="HOGENOM" id="CLU_004367_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9D2"/>
    <dbReference type="OMA" id="AMSHESM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013982">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04375">
        <property type="entry name" value="RhoGAP_DLC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000007">
        <property type="entry name" value="rho GTPase-activating protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000009">
        <property type="entry name" value="StAR related lipid transfer domain containing 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2070:FF:000001">
        <property type="entry name" value="StAR-related lipid transfer domain-containing 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12659">
        <property type="entry name" value="RHO-TYPE GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="22">
        <location>
            <begin position="635"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="23">
        <location>
            <begin position="884"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="150"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="391"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="489"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="528"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1075" mass="119246" checksum="C5F52DC0CA25C88D" modified="2014-04-16" version="1">ISNSRPSCFPFLSEIEAKEACDWLRAAGFPQYAQLYEDSQFPIEISSVTRDHDFLDGDAIEALCRRLNTLNKCAVMKLEISPQRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEELDVFSLPGEPGLFPAEGPGLTVGVSSESMLTDLSEQPEVGSIHSTHSSTGSPGPGARSADTGTLRSSSVTSVCSSGNSGANEDSLSEGLPSPKEPSSFVFSAKPGGGKSTRSKAKSFLKRMESLRLKGSPSKKKKPVSKLVISAPVLREGMDEDKLRRLNCVEISSLRGPPRNRSVSYSTQTSSSSSSPSEAGSSTVSTPSPVARARSHSAACFGRRDSSSKRGGMYLEGFDPFSHAPPGPEPPVAARNMRNQRGAAELEEEEEEEEEEEVIFFIPEGHKPGTFPRALCNSSPHRRLRRESSGDSSKGSSCGPRNRRSPAGSLDSRLSVYDNVPGSPPADGGLLLYPQLDDILQHVRGLQRVVNEWSERACEEGDSDSALDSVSPCPSSPLQHPLEEAENGSDLDSTGNSLHEQEEALASRERRDSGVGASLTRSNRPQKLRWHSFQSSHRPSVSSLTLQINCQSVVQMNLLQKFSLLKLTALLEKYTPTNKHGFSWAVPKFMKRIKVPDYKDRNVFGVPLQVNVQRMGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCPDSASYEGQSAFDVADMLKQYFRDLPEPLLTSKLSETFLQIYQYVPKDQRLQAIKAAVLLLPDENREALQTLLCFLSDVTANVQENQMTNTNLAVCLAPSLFHLNTLRRESSSPRVMHRKHSLGKPDQKDLNENLAATQGLAHMIAECRKLFQIPEEMSRCRNSYIEQGLHPLKLEELVDSSKEQPCGYKTCLKEALDSLLKEAKEKFKGYDSCSTPEQAELSYKKVSDGFPLRLWRASVEIPAAPEDVLNRVLREQHQWDEDLLDCKAVESLDQQTEVYQYVRTSMAPHPPLDHVVLRTWVTDLPKGACAVVGVSVDHEGAALMGVRANVLTSRYLIEPCGAFKSRLTYISRIDCRGHCPEWYNKIFGHWCAAEVVRIRDSFSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NA65</accession>
    <name>W5NA65_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Milk fat globule EGF and factor V/VIII domain containing a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024784">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NA65"/>
    <dbReference type="FunCoup" id="W5NA65">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017524"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017556.1">
        <property type="protein sequence ID" value="ENSLOCP00000017524.1"/>
        <property type="gene ID" value="ENSLOCG00000014224.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVK3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156049"/>
    <dbReference type="InParanoid" id="W5NA65"/>
    <dbReference type="OMA" id="CHRGCTV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014224">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF11">
        <property type="entry name" value="MILK FAT GLOBULE EGF AND FACTOR V_VIII DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="25"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="66"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="112"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="151"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="318"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="477" mass="53628" checksum="0E07E3AAAB2EDE58" modified="2025-10-08" version="2">MRHESFCHECFSVLRGTPCSRDFFSGDYCEVNYCLNGGTCVTKVGDAPFICICADGFTGEVCNETESGPCHPNPCHNDAICEVITHSRRGDMFSEYICKCPPGYSGVHCQNNVNECSSQPCKNGGTCQDLDGDYSCKCPSPYVGKYCQLRCISMLGMEGGGIAESQIMASSVHYGFLGLQRWGPELSRLNNKGLVNAWTSATHDKNPWIQVNLERKMRLTAIITQGASRMGTAEYIKSFKVAYSYDGKAFTVYKGEGQRKDKVFIGNTDNDTTKTNLFDPPIVAQYIRVIPVVCRQACTLRMEFVGCEINVYLNTAGCSEPLGMKSRLISDRQISASSAFRTWGIDAFTWLPHYARLDKQGKTNAWTALNNNRSEWLQVDLETPKRITGIITQGAKDFGNVQFVSSFKVAYSDDGQSWTIYKDEKTRKDKVFQGNIDNNIHMKNIFEPSIFARFFRVLPWTWHERITLRMELLGCED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NB02</accession>
    <name>W5NB02_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA-(apurinic or apyrimidinic site) endonuclease</fullName>
            <ecNumber evidence="21">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18 21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="18 21">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="21"/>
    <dbReference type="EMBL" id="AHAT01006664">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NB02"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017811"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017843.1">
        <property type="protein sequence ID" value="ENSLOCP00000017811.1"/>
        <property type="gene ID" value="ENSLOCG00000014467.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063540"/>
    <dbReference type="HOGENOM" id="CLU_010374_3_0_1"/>
    <dbReference type="InParanoid" id="W5NB02"/>
    <dbReference type="OMA" id="SFWICPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014467">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09088">
        <property type="entry name" value="Ape2-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000030">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF27">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 21" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18 21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 21" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="23">
        <location>
            <begin position="519"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="359"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="423"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="active site" evidence="17">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="17">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="19">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="19">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="569" mass="61966" checksum="7187F3836F8C4D55" modified="2014-04-16" version="1">MKIVSWNINGIRTFKAGIKKSLDALDADIICLQETKVTRELLDERTAIVEGYNSYFSFSKGRSGYSEGVATFCKDNATPFAAEEGLTGLLTNHSGAVGCYGDQAEFSDEELQTLDSEGRAIITQHRIVCGEREETLTVLNVYCPRADPEKPERRLFKLRFYQLLQIRAEAILGAGGHVIVLGDVNTSHRPIDHCDPDDVDDFDENPGRKWLDHFLAGRNPEEGKAGEEGEGAGTDSRDAAGGTRGGRFVDTFRHFHPARSHAFTCWSTVTGARRTNYGTRIDYILASRGLAEAEFLDSDILPEVEGSDHCPVRGLLRCALLASPRCPPLCTRHMPEFMGRQQKLSRFLVKVGQAAAATAADSQLPGSQESGEVRENLPPAAETGRGSGTKRRPLAEPSASRNKKARTATSGPRQQGNLLAFFRPRPTGAGRGATAAGDGDPLGGRQDSPARVGGTPEENELSLPGSAQPDGPVPEVEEEEEEEEEEGGRASQKPQHRDGEEPQRFWKSVLRGPPTPPSCKAHGEPCVLRTVKKPGPNLGRQFFVCNRPQGHASNPDARCNFFTWVAKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5NBD8</accession>
    <name>W5NBD8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Metastasis-associated protein MTA1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the metastasis-associated protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196585.1">
        <property type="nucleotide sequence ID" value="XM_015341099.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015196587.1">
        <property type="nucleotide sequence ID" value="XM_015341101.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NBD8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017947"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017979.1">
        <property type="protein sequence ID" value="ENSLOCP00000017947.1"/>
        <property type="gene ID" value="ENSLOCG00000014580.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693874"/>
    <dbReference type="eggNOG" id="KOG3554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234573"/>
    <dbReference type="HOGENOM" id="CLU_006585_2_1_1"/>
    <dbReference type="InParanoid" id="W5NBD8"/>
    <dbReference type="OMA" id="GYMINGK"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014580">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04709">
        <property type="entry name" value="BAH_MTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035170">
        <property type="entry name" value="MTA1_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865:SF5">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN MTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17226">
        <property type="entry name" value="MTA_R1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82061">
        <property type="entry name" value="BAH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="ELM2" evidence="13">
        <location>
            <begin position="148"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="14">
        <location>
            <begin position="266"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="701" mass="79982" checksum="6F42C903F71F810A" modified="2014-04-16" version="1">MAANMYRVGDYVYFENSSSNPYSVRRIEELNKTANGNVEAKVVCFYRRRDIPSNLIALADKHAKELEEEMEGESLQQLTEKQRHQLKHRELFLSRQLESLPATHIRGKCCVTLLNETEALFSYLEKEDCFFYCLVYDPQQKTLLADKGEIRVGPRFQADITDLLNEGEEDGRDQAELETKIWEPESPLTDKQIDQFLVIARSVGTFARALDCSSSIRQPSLHMSAAAASRDITLFHAMDTLHRTGYDMSRAITALVPQGGPVLCRDEMEEWSASEANLFEEALEKYGKDFADIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESRLKQVYIPNYNKPNPNQINVNNAKTGLVNGSSSQGPSGNAGWACESCYSIQCYQWYSWGPPNMQCRLCAICWSYWKKYGGLKMPTRLEGERPGPARNSMAPFSYWVKNANPKYTLKTKQAFYLQTAKLTRLARYLCRALLQPRRAARRPFVPINSAAIKAECTARLPEAEKQIVKLKPATRKPLEVIVKYLELHPYVKPEPVKPAVSLASHPGSTSVAKVAPVINHGSTTILGKRSYEQHNGVEGNVKKRLLMPSRGLPNHSQVRQIATLNKSYLLNGKSLERSQRMLKQGSLPPVKGRRMNWIDAPDDVFFMATNDTRKVRKLLASFDIKRAARQPYKQICIKHVQSDLETPLQLRHPVNEEPIVIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC11</accession>
    <name>W5NC11_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pseudouridine synthase</fullName>
            <ecNumber evidence="8">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Pseudouridine synthase that catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="W5NC11"/>
    <dbReference type="SMR" id="W5NC11"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018170"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018202.1">
        <property type="protein sequence ID" value="ENSLOCP00000018170.1"/>
        <property type="gene ID" value="ENSLOCG00000014755.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00420000029802"/>
    <dbReference type="HOGENOM" id="CLU_016902_12_5_1"/>
    <dbReference type="InParanoid" id="W5NC11"/>
    <dbReference type="OMA" id="QTYCKGR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014755">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000010">
        <property type="entry name" value="RNA pseudouridine synthase domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="10">
        <location>
            <begin position="181"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="410"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="491" mass="55962" checksum="51F73778CEED81BE" modified="2014-04-16" version="1">MDTSNPVERKGEADSSTAASGKIEITKRKIAEIEEPEHDSRRKKRRGGGGKKLPPGERYVPPPQKRNPGVSFSQEHFKETSYYFEGGLRKVYPYYFDFQTYCKGRWVGKSLLEVFRTEFRAEPLEYYIQAAKKGRIRLNEKPVEDLNIILKNNDFLRNTVHRHEPPVSAKPMEILVDDGELLVVDKPASLPVHPCGRFRHNTVIFILGKERGLSSLHTVHRLDRLTSGVLLFARTLEASQRLDQLVRDRQLEKEYVCRVLGVFPEGELVCEEPILVVSYKVGVCRVDPRGKPCRTVFHRLRTDGRSSVVQCRPLTGRTHQIRVHLQFLGHPILNDPIYCSAAWGPERGKGGHIGVDDEQLLRALVEEHRAKESLHLLDITEEDLPGGALMQGKEGVAHVSSCDVAENNLGSERGCTKNKENVQREELDDTGTRSESPKVVKDPLCSECKIVRKDPTEKELIMYLHALKYKGPNFEYSTRLPDWAKEEWQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND05</accession>
    <name>W5ND05_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal protein S6 kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01016924">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015207163.1">
        <property type="nucleotide sequence ID" value="XM_015351677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND05"/>
    <dbReference type="FunCoup" id="W5ND05">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018514"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018546.1">
        <property type="protein sequence ID" value="ENSLOCP00000018514.1"/>
        <property type="gene ID" value="ENSLOCG00000015032.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691210"/>
    <dbReference type="CTD" id="337670"/>
    <dbReference type="eggNOG" id="KOG0603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159242"/>
    <dbReference type="HOGENOM" id="CLU_000288_58_3_1"/>
    <dbReference type="InParanoid" id="W5ND05"/>
    <dbReference type="OMA" id="HSWITCK"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015032">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
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        <property type="term" value="F:ribosomal protein S6 kinase activity"/>
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        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
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        <property type="term" value="P:TORC1 signaling"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="AGC-kinase_C"/>
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        <property type="entry name" value="Pkinase_C"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
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        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="67"/>
            <end position="326"/>
        </location>
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            <begin position="327"/>
            <end position="396"/>
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    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="421"/>
            <end position="678"/>
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    <feature type="active site" description="Proton acceptor" evidence="13">
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            <position position="192"/>
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    <feature type="active site" description="Proton acceptor" evidence="13">
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            <position position="538"/>
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        <location>
            <begin position="73"/>
            <end position="81"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="99"/>
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            <name>ATP</name>
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            <end position="435"/>
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    </evidence>
    <sequence length="740" mass="83439" checksum="DBF8AA3C384B14D8" modified="2014-04-16" version="1">MPFAQPQDSWHKMEVHSVCSEVNGHQIMDEPMEEGESISHCDEGTIKEIPITHHVKEGCEKADPSQFELLKVLGQGSFGKVFLVRKILGPDTGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVMFTEEDVKFYLAELALALDHLHNLGIVYRDLKPENILLDEAGHIKLTDFGLSKESVDQDKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSLGVLMFEMLTGTLPFQGKDRNETMNMILKAKLGMPQFLSLEAQSLLRMLFKRNPANRLGAGSDGVEEIKRHPFFSTIDWNKLYRREIQPPFKPAAGKPDDTFCFDPEFTAKTPKDSPGVPPSANAHQLFKGFSFVAPAALEESKNASLVNILPIVQQLHAGTAQFSDMYELKEDIGVGSYSICKRCVHRVTGMEFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDAYDEGRFVYLVTELMKGGELLDKILRQKYFSEREASAVLYTIAKTVDYLHCQGVVHRDLKPSNILYMDDSGNPDSIRICDFGFAKQLRGENGLLLTPCYTANFVAPEVLMRQGYDAACDIWSLGVLLYTMLAGYTPFANGPNDTPEEILLRIGSGKFSLSGGNWDTVSETSKDLLSHMLHVDPHQRYTAEQVLKHPWIACRDQLPHFQLTRHDAPHLVKGAMAATYSALNHKTCQPVLEPVAASSLAQRRSMKKLTSTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND84</accession>
    <name>W5ND84_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Unc-45 myosin chaperone A</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004844">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015343373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND84"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018625.1">
        <property type="protein sequence ID" value="ENSLOCP00000018593.1"/>
        <property type="gene ID" value="ENSLOCG00000015105.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102691394"/>
    <dbReference type="KEGG" id="loc:102691394"/>
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    <dbReference type="eggNOG" id="KOG4151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159320"/>
    <dbReference type="HOGENOM" id="CLU_007331_0_0_1"/>
    <dbReference type="InParanoid" id="W5ND84"/>
    <dbReference type="OMA" id="LDQKHED"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015105">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000025">
        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF3">
        <property type="entry name" value="PROTEIN UNC-45 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="10"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="10">
        <location>
            <begin position="330"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="942" mass="103020" checksum="CEE0AAE541080B64" modified="2014-04-16" version="1">MSVNGKDKDPAALREDGNSLFKNGDYQGALSCYTRALRLSDSQAESAVLHRNRAACYLKLEDYSKAEADATKALDADPGDVKARFRRSQALQKLGRLDQAFLDVQRCAQMEPKNKAFQESLRQLGAQIQQKALQLSSTDARVQQMFSLLLDSSSQPSDRQKAAQNLVVLSREEAGAEQIFRNDGVRLLQRLLDSKQEDTTLSALRTLVGLCTGHQSRTMAIVNELGMERLCAVMGMKGEAVSLAACHLLQVMFEALMEGMKKEVRGKEEAILPEPSRELRSMLCHLVDVLAMEEVSGPGRDGAINLLVKQVPRKSLKDPNNSLTLWVIDQGLKKILEVSGTVPGVLDGPPLTDNTHMSCSVLLNKLYDDLKSDGERENFSRLCEQYIQHHFAAPGLNGKLRAIQAVSVLLQGPSDVGNRTLELSGIMDSIISLCASPDLSHQQVAVEALIHAAGKAKRASFITANGVTLLKDLYKKSENDRIRVRALVGLCKLGSAGGTDFSMKQFAEGSTLKLAKQCRKWLCNEALPPSSRRWAIEGLAYLTFDADVKEDLAEDRQALQAMFQLAKSEDKTVLFAVGSTLVNCTNSYDVEKPDPQLVELAKYAKQHVPEEHPKDAQAFVQKRLSKFLEAGVVSALVCMVKQESPALTESCRECVSRVFLALVERQEDRGTVVAQGGGKALIPLATEGTDVGKTKAAQALAKIAITSNPEIAFPGERIYEVVRPLVSLLALDRTLLQNFEALMALTNLAGISERLRQKIIKEKAVPKIEGYMFEEHELVRAAATECMCNLVLSTEVQELFVATGSDRLKLLVLYSGEDDERLRKAAAGTLAMLTSVQPQLCARVPATTTHWLEILQALLLSDTVELQHRGAALVLNLMQAERTLAETLMESEVLEILTVLAKGDIATQSPAAKTAKQCLDVAVEYGIIRSLGSEGTSEELLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDT6</accession>
    <name>W5NDT6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01006134">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDT6"/>
    <dbReference type="FunCoup" id="W5NDT6">
        <property type="interactions" value="1615"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018795"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018827.1">
        <property type="protein sequence ID" value="ENSLOCP00000018795.1"/>
        <property type="gene ID" value="ENSLOCG00000015274.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000140979"/>
    <dbReference type="HOGENOM" id="CLU_062828_2_2_1"/>
    <dbReference type="InParanoid" id="W5NDT6"/>
    <dbReference type="OMA" id="MNKKGAP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015274">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043045">
        <property type="term" value="P:epigenetic programming of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000005">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="129" mass="13622" checksum="92F39E7F6852A2EE" modified="2014-04-16" version="1">MIAGGKAGKDSGKAKAKAVSRSQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5NED2</accession>
    <name>W5NED2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="15">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Homodimerization via the leucine zipper domains is required for autophosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with TLR4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="15"/>
    <dbReference type="EMBL" id="AHAT01027866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027867">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01027868">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NED2"/>
    <dbReference type="FunCoup" id="W5NED2">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018991"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019023.1">
        <property type="protein sequence ID" value="ENSLOCP00000018991.1"/>
        <property type="gene ID" value="ENSLOCG00000015431.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159629"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_14_1"/>
    <dbReference type="InParanoid" id="W5NED2"/>
    <dbReference type="OMA" id="KDRASHH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015431">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14061">
        <property type="entry name" value="STKc_MLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000169">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
    </dbReference>
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        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 21"/>
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            <position position="116"/>
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    <sequence length="980" mass="108384" checksum="7636B5689E58C8DA" modified="2014-04-16" version="1">WTAVFDYEASGEDELSLRRGDVVEVLSKDAAISGDEGWWTGKIHHRVGIFPSNYVTYQPAIYRRLPGGRSVGDGESSPGSPEQISFSELVLEEIIGVGGFGKVYRGTWRGQEVAVKAARQDPDEDITATAESVKQEAKLFSMLRHPNIITLEGVCLQEPNLCLVMEYARGGTLNRALAGRRVPPHILVNWAVQIARGMHYLHEEAVVPIIHRDLKSSNILLLEKIENGDIGQKTLKITDFGLAREWYQTTKMSAAGTYSWMAPEVIKSSLFSKGSDVWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMEECWEQDPHIRPSFLAILEQLTAIEDAVMAKMPQDSFHSMQEDWKVEIQEMFDELRTKEKELRSREEELTRAALQQKSQEELLRRREQQLAEREINVLERELNILIFQLNKDKPNVKKRKGKFRRSRLKLKDGNRISLPSDFQHKITVQASPTMDKRRSLNGSSSSPPSSPTLIPRLRAIQLTLDESNRTWGRSTACKPEEFEDVKKGFRKKGRTWGPSSVQTKERANCAERVRPLSDGSNPWSTSLMKNQTSVPLAALFAEQGMCPDAPGSAEVSDSNKPKQLKFPNQVYIDLPLWKDDQGEGGAAGEGVEDTTPASSASSTPQVTPTNSLKRAAQRRRTDAVLHSCASLLASVALGLDLREVGRAPAGEEPELREEKKKREGIFQRATRFRRSTSPPGRPRKDEGGPPAPVSLLAMSAISECSSAKCLLLLPDSDGLEEALRRAGTDPPAPSAIAPQSSKNRERGRERRGGSIEPGHATLSPTRRWAKQYTEPACAHLKPVKESRIPPMKLLFLSSQANGVPSPLTDSAVLVQAASPGAVALRERQDRPDFPSSADGVSRPRPLALKSKVPSWALLRGHNKSYSLGPFSGEKSAQTFDILLQAEAQVVPGACSLLDMDTEGQKRDCTVPLCRIQSKPGRPAVYELEKEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NF59</accession>
    <name>W5NF59_LEPOC</name>
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        <submittedName>
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    </protein>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="CCAR1/CCAR2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="457"/>
        </location>
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        </location>
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        </location>
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            <end position="634"/>
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            <end position="716"/>
        </location>
    </feature>
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        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <sequence length="868" mass="95820" checksum="E1F2BA13E1FF6997" modified="2014-04-16" version="1">MSSRANHKTAPALLGHSPGFVHSSAGMEATQAPSLLQNPQLKQRVFTGVVTQLQDYYGMVDEEVQFQMSVVKGRLPQVGEKVLVTAVLDPAVSQSWTAQKVQALNSQLFFKSPPPLLPSMSSGQKQGILGNKPRPLLQDPMIPPLLPSMQQATPKPGLLQTPSHMPSWGGHFEPWTGGRKRHSEGSGRRGSRWEDGGWGGDSQHQKRRRWRGTQEEGPQKKTNVRTPRYWPLFSRFSRDSSACDTMEVLRRYPQLSIPDAFFHLRLSWVDTFPPSRPLTLGYPCPFYQGSPQPEAELTHPTSTNTTYSTKVLLLSMPSLEEIYRQCCSLSQDNKEPAEGAVHPTALIKFVVGIRGGEERWIGGPWSPSEDGANPAKEPQVLVRTAVRWAREQAGLDLSGCSQWFKLAELRYLRGGHVETTVFFLPDVWHSLPGRAEWEALRAVPDAGANSDATEGHEQQCETEPAVTPLPEEPGLVLRPCEGLSLTPLPLAALLEPRNSTSEEAFEVGLMAELFNEILQRDFGLQIYRALSAMPPGAGEGREREDVAMKEEKPGKAESGSGKKAGGERRTRRRRGGKERESTEDEEEKTDEEDDEKPEEGEEKTAESVMTVEEAETKSAVSNHSEEPASKDAERPPGWQAMQPPFLLLSFLYFDQQLSGQLAERDLEEILHSLGLHFTRAQARELVSRAVLGGVCRYRTLAARWEDTDPSDPEPPGDDSNLQGNRALLPGSGGKAASGSSRRSGTGSSDVVTHRGTVLNIANLLASLEKAERTRVWLEDRVARLAEVEAREAKREAEQAALSSELSAAQTAAGDLRGRLERAEKRNAAYERSLKENAGHMIAVIEKMQKLVDKTTNLAESRTESGEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFY5</accession>
    <name>W5NFY5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:ch211-126j24.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638628.1">
        <property type="nucleotide sequence ID" value="XM_006638565.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFY5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019576.1">
        <property type="protein sequence ID" value="ENSLOCP00000019544.1"/>
        <property type="gene ID" value="ENSLOCG00000015867.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698103"/>
    <dbReference type="KEGG" id="loc:102698103"/>
    <dbReference type="GeneTree" id="ENSGT00950000183209"/>
    <dbReference type="HOGENOM" id="CLU_013074_0_0_1"/>
    <dbReference type="OrthoDB" id="28829at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015867">
        <property type="expression patterns" value="Expressed in camera-type eye and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019381">
        <property type="entry name" value="PACS1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057541">
        <property type="entry name" value="PACS1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF14">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25332">
        <property type="entry name" value="C2_PACS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10254">
        <property type="entry name" value="Pacs-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 N-terminal C2" evidence="5">
        <location>
            <begin position="58"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 C-terminal" evidence="4">
        <location>
            <begin position="513"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="216"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="737"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="740"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="768"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="927" mass="101139" checksum="9D1D4CEB48130109" modified="2014-04-16" version="1">MMAAAVERGGVRAAFLNPGSGGGGGPAVAATPAGPGAAAAAAAAAGGSGSGSSGPMPVPMNLFATWEIDRSSPSCVPRLCSLTLKKLIVLKELDKDLSSVVIAVKIQGSKRILRSNEYALPPSGLMETDLELTFSLQYPHFLKRDANRLQIMLQRRKRYKNRTILGYKTLAVGVINMAEVMQHPTDGGQILGLHSNVKDAAVRAAEISVYSLSSQPIDHEDGSVQAGPKTKASDRSPDIDNYSEEEDDSFSSEQEASDDAVHGQDLYDEDDEVRKPKKSRRKMTRTTSMTRQPNFKQKFVALLKRFKVTDEVLDSDPVDQTQEVEEDLDLLYDSLEVYNPSDSGPEMEDNESVLSTPKPKLKPFFEGMSHSSSQTEIGSLHSQKSQPRDHTSPGGEFLPSEKSKVPGSRYQDDVIVEATPGGAEEEDLTVGDAGNSWDTNTDKLACSIGKLTKTESQNHMSPSKVESKPHRRPRSTSMKDRQNSKAQSDRTSSMDSESSPDSRLTAQVPRKSVYDQLNQILISDEQLPESIILINTTDWQGQYLSEILHEQNQPIVSTCSAADVQAAFNTIVTRIQRFCNCNSQMPPTIKVAVAGDQSYLSTILRFFVEQLANKTPDWLNYIRFLVIPIGSHPLAKYVASLDSKFNSIFMDTAWREVFFGRTEPPASESIDVAGRISQYLAGANVSHQFPISEAMLTYKQKSPDEDSCQKFIPFIGVVKVGIVEHNLSASVDSDDATAVSGSTLASPPAPTSSSYGKEIAGTPPPSPSVSSALSGAGSPASGGEVMGLQVDYWTWQGPEKKKDGEKRDVGIKNTLKSNFRSLQVSRLPGGGELTPPPTMSMTVVTKEKNKKVIFLSKKPKEKELDSKSQVIDGISRLICTAKHQHTMLRVSIDGVEWNDVKFFQLAAQWPTHVKHFPVGIFGYMKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGA0</accession>
    <name>W5NGA0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01001621">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGA0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019691.1">
        <property type="protein sequence ID" value="ENSLOCP00000019659.1"/>
        <property type="gene ID" value="ENSLOCG00000015966.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SMP4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000116905"/>
    <dbReference type="HOGENOM" id="CLU_677143_0_0_1"/>
    <dbReference type="InParanoid" id="W5NGA0"/>
    <dbReference type="OMA" id="HGLVAFC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015966">
        <property type="expression patterns" value="Expressed in mesonephros and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12573">
        <property type="entry name" value="AT09986P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12573:SF4">
        <property type="entry name" value="AT09986P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41031" checksum="FF1F3FA81149271A" modified="2014-04-16" version="1">AKALGELNTEETCWWFTKLGLQKYIPFIRGANLRGSHIASIDLEILDLLHVSTPEERECLLSAVYRELHPPDTTTQELDTLLGRKTIGSYNVEKFTAALVNLKEISSKVENREQADPSHSVCNQNQQMDLENKEDKRRDLSQQVDRLQKVIYQVQGLHQSLASFCAELRDTDAEADVEGMCLGDTVVGLQQTERKRTEKELLVGGLRQRLSSLRAHTSGQRQKYESPIEVHLFDKMKLDCQIFMEEINIIFLNQQMARLKSAHQICKVKARDNMTSCTLGQLLSHQPPAVLLAIQETATAGTYGFRVQLKEGSGLVVVDVGNSKLCVNDRLVEVNGVSVVDYREEELKPLLSCQSCLIVVLRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGS1</accession>
    <name>W5NGS1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Kinesin family member 21A</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NGS1">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019830"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019863.1">
        <property type="protein sequence ID" value="ENSLOCP00000019830.1"/>
        <property type="gene ID" value="ENSLOCG00000016087.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0244">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155323"/>
    <dbReference type="InParanoid" id="W5NGS1"/>
    <dbReference type="OMA" id="QVLQNLX"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016087">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22263">
        <property type="entry name" value="Rcc_KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000233">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000011">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001310">
        <property type="entry name" value="kinesin-like protein KIF21A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056533">
        <property type="entry name" value="KIF21A/B_hel_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056532">
        <property type="entry name" value="KIF21A/B_hel_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF31">
        <property type="entry name" value="KINESIN FAMILY MEMBER 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23203">
        <property type="entry name" value="KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23204">
        <property type="entry name" value="KIF21A_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 16" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="20">
        <location>
            <begin position="3"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="1318"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="1514"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="1597"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="548"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="776"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="842"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1082"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1175"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="374"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="911"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="555"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="595"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1214"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1234"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1649" mass="184227" checksum="8E9FE239968470D0" modified="2014-04-16" version="1">NDFNYSFIRIRPQLAREKIEGCHICTFVTPGEPQVILGKDKAFTFDYVFDMETQQDAIYSNCTEKLIEGCFEGYNATIFAYGQTGSGKTYSMGTGFDVSIGENELGIIPRAVHHLFKGIEERKQAATEQGLPPPEFKVNAQFLELYNEEVLDLFDSTRDIEAKKQKSHIKIHEDASGGIYTVGVTTRTVGSETEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHLCQVRVCASTNNQDNETDNRIINGSSQIDEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACISPSDRDFMETLNTLKYANRARNIKNRVMVNQDKASQQISALRTEIARLQMELVEYKTGKRIIGEDGMESINDMFHENSMLQTENNNLRVRIKAMQETIDAQRTRLTQILSDQANQVLARAGEGNEEIGNMIQNYIKEIEDLRAKLLESEAVSENLRKNLSRASTRTSFYGGPSSYTTSLMGPENETTDIIELAKKDLEKLKKKERKKKKRLQQLEESRREEESVIKEEAPDNEQDKGVEKEVAERSSHEAEMEGQDVSDHEEGEGEEEEEEEDEMEVDDSSDDSDSELDEKANFQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEQKLMMLQSKIRDTQLERDRVLQNMGSMESYSEDKANKIKTEYEKKLSVMNKEMHKLQSAQKEHARLLKNQSQYEKQLKKLQQEVAEMKKTKVRLMKQMREEQEKARMAESRRNREIASLKKDQRKQEHQLRLLEAQKRQHELILRRKTEEVTALRRQVRPVSGKVNRKLSLPEPVQDSAHSLRAPSSRMQSAGSVAATYGNRKYQRKVVGVYSSKTARMKWQSLERRISDIIMQRMTIANMEADMNRLLKQREELAKRREKFSKKRDKIALEGSDADKTVLSLNEEMESLTANIDYINDSISDCQANIMQMEEAKEEGDTVDVTAVISSCTLSEARFLLDHFLSMAINKGFQAAQKESQIKVMEGRLKQTEINSATQNQLLFHMLKEKAEFNPELDALLGNALQELGSIPVENGDESSSDESPQSPGTEGNSLASDLMKLCGETKPRNKARRRTTTQMELLYAHSSDPALDATPEGFSAPLLPVAEAQESGEDAEIARAAARERDPLPSPSGPPFKQGGISRQSTGTEKRAPEPSPLTRRKMYEMEQMAGEKAKRKEVKHSNSGSPETSLIDPSSPPSRSCSEQNVFSRLAVSQGNSEAQPMALLLCNRADESDSSLLEVHRGVINPFPTTKSNRTALLQCIHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCSSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTPGDACAANTNRTVTIPAGENQINQIALNPTGTFLYAAAGNAVRMWDLKSIRFLSTGKLTGHLGPVMCLTVDHTGTGQDLIITGSKDHYIKMFDVSEGALGTVSPTHNFEPPHYDGIESLVIQGDSLFSGSRDNGIKKWDLSRKDLLQQVPNAHRDWVCALGIVPGNPALLSGCRAGVLKLWHSDTLSPLGEIKGHESPINAISTNSSHIFTAADDRTVKIWRARGSLDGQMSDAVDTTDEVSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHQ6</accession>
    <name>W5NHQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras homolog family member Q</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001684">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHQ6"/>
    <dbReference type="FunCoup" id="W5NHQ6">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020165"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020198.1">
        <property type="protein sequence ID" value="ENSLOCP00000020165.1"/>
        <property type="gene ID" value="ENSLOCG00000016335.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155970"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_3_1"/>
    <dbReference type="InParanoid" id="W5NHQ6"/>
    <dbReference type="OMA" id="AESYAEC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016335">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04135">
        <property type="entry name" value="Tc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000438">
        <property type="entry name" value="Rho-related GTP-binding protein RhoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="207" mass="22900" checksum="166FE67715A8024F" modified="2014-04-16" version="1">RIMANGTGTIMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVREEWVPELREYAPSVPFLLIGTQIDLRDDPKTIAKLNDMKEKPIATEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKNALKRRLGSRCINCCLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NHY2</accession>
    <name>W5NHY2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12 14">Decorin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 14">May affect the rate of fibrils formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 14">Secreted</location>
            <location evidence="1 12 14">Extracellular space</location>
            <location evidence="1 12 14">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 14">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208366.1">
        <property type="nucleotide sequence ID" value="XM_015352880.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015208367.1">
        <property type="nucleotide sequence ID" value="XM_015352881.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NHY2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020241"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020275.1">
        <property type="protein sequence ID" value="ENSLOCP00000020241.1"/>
        <property type="gene ID" value="ENSLOCG00000016391.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683252"/>
    <dbReference type="KEGG" id="loc:102683252"/>
    <dbReference type="CTD" id="1634"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158382"/>
    <dbReference type="HOGENOM" id="CLU_000288_186_0_1"/>
    <dbReference type="InParanoid" id="W5NHY2"/>
    <dbReference type="OMA" id="FRCIYER"/>
    <dbReference type="OrthoDB" id="1111193at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016391">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016352">
        <property type="entry name" value="SLRP_I_decor/aspor/byglycan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF14">
        <property type="entry name" value="DECORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002490">
        <property type="entry name" value="SLRP_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12 14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011023464" description="Decorin" evidence="12 14">
        <location>
            <begin position="17"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="15">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="52"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002490-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="359" mass="40198" checksum="CFFA6B6002ECB877" modified="2014-04-16" version="1">MKSACLSLLLVTVCWALPFRQSGFLDFMFEDGSGDITPTPTLDMQPPAGPVCPFRCQCHLRVIQCSDLGLKNVPEDIPSDTSLLDLQNNKITEIKENDFKNLKSLHALILVNNKITIIHPKAFVPLMKLQRLYLSKNLLKDMPVNMPKNLQELRIHENAITRIKRSSFEGMVNVIVMELGSNPLKSGGIENNAFLDLKRLSYIRISDTNITSLPKGLPSSLSELHLDGNKISKVDADTLKGLKNLAKLGLSFNEITTVENGSLANIPHLRELHLDNNLLTHVPPGLPEHKYIQVIYLHNNKIAAVGTEDFCPPGYNTKKAMYSGISLFSNPVPYWEVQPVTFRCVFDRSAIQLGNYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIU6</accession>
    <name>W5NIU6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Exonuclease 1</fullName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">5'-&gt;3' double-stranded DNA exonuclease which may also contain a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for DNA mismatch repair (MMR).</text>
    </comment>
    <comment type="function">
        <text evidence="17">5'-&gt;3' double-stranded DNA exonuclease which may also possess a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01001732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001733">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIU6"/>
    <dbReference type="FunCoup" id="W5NIU6">
        <property type="interactions" value="736"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000020555"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020590.1">
        <property type="protein sequence ID" value="ENSLOCP00000020555.1"/>
        <property type="gene ID" value="ENSLOCG00000016639.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047676"/>
    <dbReference type="HOGENOM" id="CLU_009851_0_0_1"/>
    <dbReference type="InParanoid" id="W5NIU6"/>
    <dbReference type="OMA" id="QDFTETK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016639">
        <property type="expression patterns" value="Expressed in embryo and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
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        <property type="entry name" value="XPGRADSUPER"/>
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        <property type="entry name" value="PIN domain-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="XPG_2"/>
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    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 17" id="KW-0228">DNA excision</keyword>
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    <keyword evidence="13 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12 17" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="10 17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
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            <begin position="704"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="797" mass="87344" checksum="60E4149408789959" modified="2014-04-16" version="1">MGISGLLQFIKEASEPVNVKKYKGQTAAVDTYCWLHKGAFSCAEKLAKGEPTDQYVTYCMKLVDMLLTHGIKPILVFDGRNLPSKKDVEKARRERREANLRQGRQLLREGKVSEARDCFTRCVDITPAMAHDVIKAARGRGVDCVVAPYEADAQLAYLNKRGIAQVVITEDSDLLAFGCTKVLLKMDRFGNGLEIAQSCLGKCKSLGDVFTEEKFRYMCILSGCDYLASIHGIGLGKACKLLKMANNPDITKVIKKMGQYLNMSVTVSDEYIEGFIKANNTFLYQLVFDPLSRRLVPLNPYPEEINPQSLSYAGPNMGDQAALQIALGNVDVNSMKKIDDYNPDITQPVKPRSRSWTDARVQQAPCELSIWSREYNPAGNTRLLSQPSEPRSPETLSTRGKEKVISTQPLKLPTRQAQVKRPRAESSMSEEVLLGQYSCSDTKKPRREAGTPPAGSSMGAGERASGPASQHRARNRFATVLQRRNQEEGPAEEQGLCSRFFCTTSSSVEPEAKQESPVKSHEPVPEVLYVSGQHSETSDVLTSTDTEFQGKGSSRAAGETTPLGCQSPCTSSRGSGVFGWSGSLGEKPKNLTSGGLVALQQFQRMKESFSWSAPRRKPSALSGSLPDKENRPGLENGEPRDSGESAYFSQTSRTSRRESITSQTRLRDNQDSETGSQESDSSDGVNRSPVKDKNTCTLIKAKVSGLSRASAGGRGAAGKLRARAPARASGLRKRGSTNDENRPGLQATISDMWKNFGFRAEKDRRRPCRKGEPMSPVKDNVQSPAPESGQDIYAVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5NJX0</accession>
    <name>W5NJX0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NJX0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020965.1">
        <property type="protein sequence ID" value="ENSLOCP00000020929.1"/>
        <property type="gene ID" value="ENSLOCG00000016929.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182881"/>
    <dbReference type="HOGENOM" id="CLU_005230_0_2_1"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016929">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF26">
        <property type="entry name" value="V-TYPE PROTON ATPASE 116 KDA SUBUNIT A 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="565"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="577"/>
            <end position="600"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="778"/>
            <end position="802"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="846" mass="96425" checksum="900D92C84D14DF8F" modified="2014-04-16" version="1">MAGSLFRSEEMCLTQIILQVEAAYCCIAELGELGLVQFRDLNAHVNSFQRKYVNEVRRCETLERILRFLEKQMELEQVVAENCGESCPLSPLPREVHDLESVLEKLESELLGMAQNQQSLRSNAMELIELKELVKTVQEFFEKETHFPDEFFTEENTALVESHGPSTAVASGKLGIIGGVIRSERMLSFERVLWRVCRGNVYIKQRDMDATVEDPDTKEEVKKKVFFIFFQGEQLGLKIKKICQGFHATVYPCPENSKERRETAASINCQLEDLQAVLKQAGDHQKRVLQEAASSIQEWKIKVQKMKAVYHTLNMCNIDVTQQCLIAETWCPVTDMDRIKTALQKGMDRSGSTVDPIVTEVSTRMSPPTFNRTNKFTAGFQNIVDAYGVSTYREINPAPYTIITFPFLFAVMFGDWGHGVIMLSFALLMIHNEKMFSKKKSNNEIWNTFFGGRYLILLMGIFSIYTGFIYNDCFAKSFNLFGSSWHVRPMFLNGTWNAHILEGSLLLQLDPAVPGVYSGSPYAFGIDPIWNIASNKLTFLNSYKMKMSVVLGIIQMIFGVILSLFNHMHFKNTVNIVFQFIPEIIFITCLFGYMVFMIIFKWCQYDVHSSQIAPSILIHYINMFLFNYGDSSNAPLYSYQQEVQSFLVVLAVIAVPWMLLFKPLILRAKHLKSQQQKNSSPLHQSVSENTEQIEMDKLQTHSSINSGGHNEIHDEGQKEFNFGDVFVNQSIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWHMVIHIGLANRSWGGVIGVFVIFSAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYTGTGYNFSPFDFKHVLSGSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLE8</accession>
    <name>W5NLE8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor 3B subunit 5</fullName>
            <shortName evidence="2">SF3b5</shortName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SF3B5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027877">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015217892.1">
        <property type="nucleotide sequence ID" value="XM_015362406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLE8"/>
    <dbReference type="FunCoup" id="W5NLE8">
        <property type="interactions" value="1038"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021457"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021494.1">
        <property type="protein sequence ID" value="ENSLOCP00000021457.1"/>
        <property type="gene ID" value="ENSLOCG00000017352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683407"/>
    <dbReference type="KEGG" id="loc:102683407"/>
    <dbReference type="CTD" id="83443"/>
    <dbReference type="eggNOG" id="KOG3485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013215"/>
    <dbReference type="HOGENOM" id="CLU_138804_3_1_1"/>
    <dbReference type="InParanoid" id="W5NLE8"/>
    <dbReference type="OMA" id="YDRFNIH"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017352">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017089">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037010">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="site" description="Interaction with RNA" evidence="3">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="3">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037010-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="86" mass="10079" checksum="20250C4A55EEEE90" modified="2014-04-16" version="1">MTDRYNIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFSVAENESKARVRFNLMEKMLQPCGPPADKPDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLK4</accession>
    <name>W5NLK4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Zinc finger and BTB domain containing 38</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015290">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NLK4"/>
    <dbReference type="FunCoup" id="W5NLK4">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021513"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021550.1">
        <property type="protein sequence ID" value="ENSLOCP00000021513.1"/>
        <property type="gene ID" value="ENSLOCG00000017408.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161449"/>
    <dbReference type="HOGENOM" id="CLU_007011_0_0_1"/>
    <dbReference type="InParanoid" id="W5NLK4"/>
    <dbReference type="OMA" id="GSCFDNT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017408">
        <property type="expression patterns" value="Expressed in heart and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000235">
        <property type="entry name" value="Zinc finger and BTB domain containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000437">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF58">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="18">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="535"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1094"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="870"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="880"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="937"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="983"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1157" mass="128235" checksum="FA5EDB3A3448734A" modified="2014-04-16" version="1">MTVMSPASESLTDSSHFQTVLCCLNDQRIQGMFCDVTIVVEDIKFKAHKNVLAASSFYFKNAFSSQEFWVSGQVLELLDLKSDTFAGILNFIYSAKVALTGSEDVKLLVAAGKKLGIPFLENLAGQEKRSSNLDAVQKASPDSTLLCTESHSLKKETPNSSNEPRTEESECASGPRITNAFSIFEAGPNDELFSPLDLRANAKRSQDPENVPEGCPTQSVGAADCEQTQALSEHSYAANQMRKTTEQSETELNDGRKESFVPTRVLTKPMMSLSCSPLKKRHRLSSNLIKTTVVQSSESPLAFPNTTGGPVATSESQNDPPSSAIGSLAENTPAMETSTPALETLTDPSQNVDRCEYCNETFSNNALFHMHIQVHKKRFVSHLSCKFCRKKFMHLKRLHNHEQICSKAPILESEHESSCDQVLKGSDADNISSTKDNTPPTCHEGISDSNSRSSFQMEASETLQQLHRTMKVVGGHRTYVCCVCKRSYVTLSSLRRHENVHSWQRTYPCHYCNKVFALAEYRTKHEIWHTGERRYQCIFCLETFMTYYILKNHQKSFHGIDPRLAVNKKSSNGGFKSSIYPIKLYRLLPMKFRKRQYKTYSQTFAESTECNSQAFPVSVAESSQNSLNCRSPSSGFEENILMSGTEGGCVGQPLFSMPVTFMATPKVVASVAPHITFDHTGGTQPISLEADTCPQKTVNSTSHDTQVFDGMASNNNNKGSSITSYGSKVPSVIMQTRPASVIVCGNDAGSIAAHNNNDDGSPMPFLEMNTASLPGSNNDKGQGGARLEGLSAVQGTETAVSQVSQPAYSVASDRTASAHCMGSKTETYIAKPACPGPSLDSQVPPLCQITVKIGDEAIIRRRIKGTKLFQRKRKKCKQNHLPDEDRSALTGPKEKTGKNVCLRSRTDVTPIIEPEPYDDMTDRDTGDKLWRPYYSYKPKKKPKGANKPRSKHRRRGRRGRTPGRSSRSSEKTSDPAGADHYLDTSSNAHGSISSDMTGNGKHLRKSIHKDAYSCDICKSLFSSLSTLRTHIISCRPSEPLYVCKTCGKRCPSSEVQSADSPYLHGDRDFACKNCMEDGSCFNNTFKNHNTEKRYRCSFCPQRFLYLATKKSHEKKHVEQPGKGYNCYYCPKVCKSSATLEIHTRKHCIKTEEEEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5NM95</accession>
    <name>W5NM95_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 51F2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5NM95"/>
    <dbReference type="FunCoup" id="W5NM95">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021754"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021791.1">
        <property type="protein sequence ID" value="ENSLOCP00000021754.1"/>
        <property type="gene ID" value="ENSLOCG00000017649.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RP7I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="HOGENOM" id="CLU_012526_0_1_1"/>
    <dbReference type="InParanoid" id="W5NM95"/>
    <dbReference type="OMA" id="RIACNDY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050911">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of smell"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF966">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="44"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="321" mass="36306" checksum="5B9DD23D0F17C2BB" modified="2014-04-16" version="1">NSTLPQNVSFVRPQFFYIGGFSNIPHVKYYYIFLCFVFIVTVLGNSFIMFIIYMEQSLHTPKYVAVFHLAVVDMCGSTALIPKMIETFLFDFQFITYEACLANMFFVHFLSSLQSLTIVILAYDRFVAICFPLKYHIIITTAGITKILAGIWILAAAVILSLVGLTTRLSFCKTIVIDSYFCDHGPMYRMSCNDNFPNYVIAQMGITILLAAPLTLILSSYVCIILALLKIASTDERLRAMKTCTSHLILVAMFYLPISAIYIAANLTIIHPNIRIISTSLSSTIPPMMNPIIYTIKTEEVMKAVRKLQKRNKAVKLKEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMA4</accession>
    <name>W5NMA4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-5AC-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NMA4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021763"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021800.1">
        <property type="protein sequence ID" value="ENSLOCP00000021763.1"/>
        <property type="gene ID" value="ENSLOCG00000017658.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102690169"/>
    <dbReference type="eggNOG" id="ENOG502TDK5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692538_0_0_1"/>
    <dbReference type="InParanoid" id="W5NMA4"/>
    <dbReference type="OMA" id="NMETITP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017658">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867824" evidence="3">
        <location>
            <begin position="20"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="398" mass="39063" checksum="C6C4A86FB7539527" modified="2014-04-16" version="1">MARAARALVLALVLATVRAEPGNDTETANPTLMVHHLTTAEPLILTTMAPSTALQTATPDSTAGPPETSTKPTAALLGDTEPPSSPTSTGATTAPSTALTSWPTTLETTSTALTSPPTTPETTSTAGADTTSTAGFSPTSSGPPTSPETTAAPPAGLPGDTTADASTESTTTASTTTAATTTAATTTSRVSSTTPEVAPSTAASGAAGAGTAPVSSLLLWIVVAVLVGAVLLVGCVAAGVYVCKRRRSGSTTFPGAYSGGRQSRKHKGGQDVWAAPMPDKEENGGPAGDEDAEAGRGKGPEGDKEAGDPGALSTFLNRAGSRPTSGAQPEGVSVELREEGAARGEAQPLLGPDPGAPLENGQGPAPAPLPDSSALPPPPPAEQGGDGLSPTFCLTSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMC2</accession>
    <name>W5NMC2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01019607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMC2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021781"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021818.1">
        <property type="protein sequence ID" value="ENSLOCP00000021781.1"/>
        <property type="gene ID" value="ENSLOCG00000017676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00830000128446"/>
    <dbReference type="HOGENOM" id="CLU_077975_8_1_1"/>
    <dbReference type="InParanoid" id="W5NMC2"/>
    <dbReference type="OMA" id="WKEAREW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017676">
        <property type="expression patterns" value="Expressed in intestine and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002173">
        <property type="entry name" value="TRADV8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004869549" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="114" mass="12885" checksum="949B9677C9984E54" modified="2014-04-16" version="1">TSTIYFFFFFPAMAAGDSITSQETDLSGNEGDSVTLTCSYDSSSNDIYLFWYRQSSDQPLQYILRKGARSYSGTNTAEFAKRRFSSTAERYSTTLTITGLTLEDTAIYYCALWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMQ0</accession>
    <name>W5NMQ0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMQ0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021909"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021947.1">
        <property type="protein sequence ID" value="ENSLOCP00000021909.1"/>
        <property type="gene ID" value="ENSLOCG00000017805.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QWM8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234513"/>
    <dbReference type="HOGENOM" id="CLU_037388_4_1_1"/>
    <dbReference type="InParanoid" id="W5NMQ0"/>
    <dbReference type="OMA" id="PNHAIKE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017805">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990349">
        <property type="term" value="P:gap junction-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF46">
        <property type="entry name" value="GAP JUNCTION BETA-2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="5" id="KW-1009">Hearing</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="146"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="264" mass="30419" checksum="D713D3D310091BCC" modified="2014-04-16" version="1">MSWGQLYALLGGVNKHSTSLGKVWLSVVFVFRIMILVVAAESVWGDEQSDFTCNTLQPGCKNVCYDRYFPISHIRLWCLQLIFVSTPALLVTMHVAYRKRGFKRDILRAHGEKAEEDLEELRKKRMHITGPLWWTYTTSLFFRLLFETAFIYIFYYIYSGFQMPRLVKCTDWPCPNTVDCFISRPTEKTVFSIFMIATSGICIILNVAELGYLVAKALMRSCGGQDKPSFHPRHVSREKERLQNKANELLLSETCSLKSSAPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5NN52</accession>
    <name>W5NN52_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3</fullName>
            <ecNumber evidence="14">2.4.1.102</ecNumber>
            <ecNumber evidence="13">2.4.1.148</ecNumber>
            <ecNumber evidence="12">2.4.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">C2GnT-mucin type</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Also has I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-[N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56188"/>
            <dbReference type="Rhea" id="RHEA-COMP:11691"/>
            <dbReference type="Rhea" id="RHEA-COMP:14412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:87079"/>
            <dbReference type="ChEBI" id="CHEBI:139581"/>
            <dbReference type="EC" id="2.4.1.148"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 3-O-[N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56192"/>
            <dbReference type="Rhea" id="RHEA-COMP:11692"/>
            <dbReference type="Rhea" id="RHEA-COMP:14413"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:87080"/>
            <dbReference type="ChEBI" id="CHEBI:139580"/>
            <dbReference type="EC" id="2.4.1.148"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 3-O-[beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56212"/>
            <dbReference type="Rhea" id="RHEA-COMP:13922"/>
            <dbReference type="Rhea" id="RHEA-COMP:14419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137949"/>
            <dbReference type="ChEBI" id="CHEBI:139605"/>
            <dbReference type="EC" id="2.4.1.102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 3-O-[beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = a 3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56216"/>
            <dbReference type="Rhea" id="RHEA-COMP:13923"/>
            <dbReference type="Rhea" id="RHEA-COMP:14420"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137950"/>
            <dbReference type="ChEBI" id="CHEBI:139607"/>
            <dbReference type="EC" id="2.4.1.102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + UDP-N-acetyl-alpha-D-glucosamine = a beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;6)]-beta-D-Gal-(1-&gt;4)-N-acetyl-beta-D-GlcNAc derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:138371"/>
            <dbReference type="ChEBI" id="CHEBI:138372"/>
            <dbReference type="EC" id="2.4.1.150"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyltransferase 14 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.102" evidence="14"/>
    <dbReference type="EC" id="2.4.1.148" evidence="13"/>
    <dbReference type="EC" id="2.4.1.150" evidence="12"/>
    <dbReference type="EMBL" id="AHAT01004920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NN52"/>
    <dbReference type="FunCoup" id="W5NN52">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000022061"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022100.1">
        <property type="protein sequence ID" value="ENSLOCP00000022061.1"/>
        <property type="gene ID" value="ENSLOCG00000017958.1"/>
    </dbReference>
    <dbReference type="KEGG" id="loc:102683301"/>
    <dbReference type="eggNOG" id="KOG0799">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159331"/>
    <dbReference type="HOGENOM" id="CLU_032341_1_2_1"/>
    <dbReference type="InParanoid" id="W5NN52"/>
    <dbReference type="OMA" id="NMTRDCE"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017958">
        <property type="expression patterns" value="Expressed in intestine and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047225">
        <property type="term" value="F:acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003829">
        <property type="term" value="F:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008109">
        <property type="term" value="F:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297:SF81">
        <property type="entry name" value="BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00055416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="443" mass="51703" checksum="EA1D79158CDEB254" modified="2014-04-16" version="1">MVQWMKFKKMTNSTSGWKRHKFTNTAAFSFICLSFTVILWQTKKLTCPLDDLHPRKIRQLEEEKDILTCSRIIQGDTEAIEEALRSRILVANKKKPLTEAYFLSITQNCEAYVQERKFITFPLSKEEKDFPIAYSMVIHEKIETFERLLRAIYTPQNVYCVHVDQKSSEEFKNAVKAIASCFPNVFLARKLEKVIYATWSRVQADLNCMEDLLNSTVQWRYLLNTCGTDFPIKTNLEIVHALKALNGRNSLESESTPRHKKDRWLYHYEVKDHISRTETKKSPPPIGSPMFSGNAYFVVTRDFVKHVFENPETQKLLEWEKDTYSPDEHLWATLQRMSGVPGSNPINEKFHTSDMIAIARLVKWEYLEGDIQKGSSYPRCTGVHRRSVCVYGSGDLHWMLHQHHLFANKFDPEVDDTAIKCLEVYLRLKALHNRDLHVQYAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVV9</accession>
    <name>W5LVV9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVV9"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000266.1">
        <property type="protein sequence ID" value="ENSLOCP00000000266.1"/>
        <property type="gene ID" value="ENSLOCG00000000241.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S9SA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115955"/>
    <dbReference type="HOGENOM" id="CLU_1312674_0_0_1"/>
    <dbReference type="InParanoid" id="W5LVV9"/>
    <dbReference type="OMA" id="PTHRTGN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000241">
        <property type="expression patterns" value="Expressed in larva and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEA" evidence="2">
        <location>
            <begin position="96"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="213" mass="22414" checksum="1D7F00DA98D0E228" modified="2014-04-16" version="1">ATSPQVNTPGTPENTTSPHVNTTGTPENTSPHVNTTSTPENTTPPYGNTPGTSENTPPPHVSTPGTPDNATSPHINSTGTPENSPSPPANSSPPPPISRPIVVLQFSLNQDFNEDLKNKTSEAFQSLAKNITTQLDTVYRNKFGDAFNRTEITDFRSGSVIVDSTLIFNNLTVVPNTSEVTNTLKAAANTSGFSLPVNTSTIVASALMSTPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW94</accession>
    <name>W5LW94_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW94"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000401"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000401.1">
        <property type="protein sequence ID" value="ENSLOCP00000000401.1"/>
        <property type="gene ID" value="ENSLOCG00000000369.1"/>
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    <dbReference type="InParanoid" id="W5LW94"/>
    <dbReference type="OMA" id="AITINNY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF29">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25328" checksum="84C1E462E4A7CE1B" modified="2014-04-16" version="1">MSKLEQKVLFAQDVLLTNHNEVKCHTTLNLFAEAYQSLILSLKIRKTKILFQDVPDQITEPSAITIAGEMVEVVDHFPYLGSHLSQKATIEEEVQHRISYASYASISFGKLRHRVFENCDLKMETRIKVYKAVCIIALLYGNEAWVTYCCHLKTLERFHQCCFQRWEDSRTNSSVLIEEKMRSIEAMIPQNQLCWTGYYIRMPNSHLPKQVLYFQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWF8</accession>
    <name>W5LWF8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWF8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000465.1">
        <property type="protein sequence ID" value="ENSLOCP00000000465.1"/>
        <property type="gene ID" value="ENSLOCG00000000387.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="HOGENOM" id="CLU_723532_0_0_1"/>
    <dbReference type="InParanoid" id="W5LWF8"/>
    <dbReference type="OMA" id="VAHYVEP"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RBD_ZCCHC3_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23057">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="387" mass="43200" checksum="2C2454BAF2E7DE9B" modified="2014-04-16" version="1">IALPSLEEESKHRGRARVPTPNQEAKEAEEEKVGEERGWGYGVPSLSGEKGDFFHREPGRSEDVSPAPSGGVGEDLPVASRPVADEPLGQVQGKRRVPTDSAADQAPTEGSGGDQLESRKRKMEVALKGCLPARTGVAEFSGHFSIKMERGAASREGRVRYAVRFMLRADGERTELVSRDEFEEDVLSKALHLEPEDVFCLQDFPGKKCLDVSLVSEGLVRGVWDIVNRKKEEKPFVAHYVEPLFARELKTLTVAMLNPYVPEEDIVCFLKRFVDIQGLGVKILDKKGFWTGKRRFKVRFRANGSAPDGLLHPPATFEIGPNRGYLYCYGQPLVCRRCRKEGHNMMVDCRECVCRRCEGVGHFTSECTEEIKYNLCGGEGHVFRDCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXL1</accession>
    <name>W5LXL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01036782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXL1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000868"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000872.1">
        <property type="protein sequence ID" value="ENSLOCP00000000868.1"/>
        <property type="gene ID" value="ENSLOCG00000000783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163847"/>
    <dbReference type="HOGENOM" id="CLU_077975_5_2_1"/>
    <dbReference type="InParanoid" id="W5LXL1"/>
    <dbReference type="OMA" id="EYHADAI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000783">
        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002624">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865258" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="126" mass="14242" checksum="5A9A885240C8734B" modified="2014-04-16" version="1">MMFLLTSLALYLWAHCSLWTCADSSIVLSQTASEVQRPGEALKLSCEVTGFDVNSYWMVWVRQTAPGKALEWLASIGGRSDTEYHADAIKGRFRASKDFSRHIFFLQMNSLKAEDTAVYYCAKESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ45</accession>
    <name>W5LZ45_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3</fullName>
            <ecNumber evidence="24">1.14.11.33</ecNumber>
            <ecNumber evidence="23">1.14.11.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Alkylated DNA repair protein alkB homolog 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 3,N(4)-etheno-2'-deoxycytidine in single-stranded DNA + 2-oxoglutarate + O2 + H2O = a 2'-deoxycytidine in single-stranded DNA + glyoxal + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:70471"/>
            <dbReference type="Rhea" id="RHEA-COMP:12846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17906"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:34779"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:189585"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:70472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:30299"/>
            <dbReference type="Rhea" id="RHEA-COMP:12192"/>
            <dbReference type="Rhea" id="RHEA-COMP:12193"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:32875"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="1.14.11.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:30300"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N(1)-methyl-2'-deoxyadenosine in single-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in single-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70447"/>
            <dbReference type="Rhea" id="RHEA-COMP:17895"/>
            <dbReference type="Rhea" id="RHEA-COMP:17896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:139096"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70448"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N(1)-methyladenosine in mRNA + 2-oxoglutarate + O2 = an adenosine in mRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49516"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12415"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="1.14.11.54"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an N(3)-methyl-2'-deoxycytidine in single-stranded DNA + 2-oxoglutarate + O2 = a 2'-deoxycytidine in single-stranded DNA + formaldehyde + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70435"/>
            <dbReference type="Rhea" id="RHEA-COMP:12846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17894"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:139075"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:70436"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with the ASCC complex composed of ASCC1, ASCC2 and ASCC3. Interacts directly with ASCC3, and is thereby recruited to the ASCC complex. Interacts with OTUD4; the interaction is direct. Interacts with USP7 and USP9X.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.33" evidence="24"/>
    <dbReference type="EC" id="1.14.11.54" evidence="23"/>
    <dbReference type="EMBL" id="AHAT01008086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ45"/>
    <dbReference type="SMR" id="W5LZ45"/>
    <dbReference type="FunCoup" id="W5LZ45">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001407.1">
        <property type="protein sequence ID" value="ENSLOCP00000001402.1"/>
        <property type="gene ID" value="ENSLOCG00000001237.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QW9E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157226"/>
    <dbReference type="HOGENOM" id="CLU_048788_2_1_1"/>
    <dbReference type="InParanoid" id="W5LZ45"/>
    <dbReference type="OMA" id="FEFHQPT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001237">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990930">
        <property type="term" value="F:mRNA N1-methyladenosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000003">
        <property type="entry name" value="alpha-ketoglutarate-dependent dioxygenase alkB homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="15" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0558">Oxidation</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="28">
        <location>
            <begin position="166"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00054625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00064884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00066588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00071421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00077988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="275" mass="31301" checksum="F49A46EC14331076" modified="2014-04-16" version="1">MGDKRQRARVQGAWARALPKAPNPRETPGVISSPSTAPNCWGPEEVKFEFQQPTQLRREIPAEKVIEKEGEFVISQGPTGISRLRLFPSFLSVGEADWMFARLLEELPWQQKSNMHQGVLYDEPRLTCWFGELPYTYSNSTLQANPEWHPVLLELKGQVELASGHSFNSLLCNLYRDGKDSIAWHSDSEPSLGARPTIASLSLGDTRTFQMRKQPAAEDRGDHTYVERLRVPLAHGTLLLMEGAVQEDWQHRVPKEYHDRGARINLTFRTVYPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZ80</accession>
    <name>W5LZ80_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008086">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZ80"/>
    <dbReference type="FunCoup" id="W5LZ80">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001437"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001442.1">
        <property type="protein sequence ID" value="ENSLOCP00000001437.1"/>
        <property type="gene ID" value="ENSLOCG00000001253.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164760"/>
    <dbReference type="HOGENOM" id="CLU_313728_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZ80"/>
    <dbReference type="OMA" id="SYHHQFT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001253">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF17">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="5">
        <location>
            <begin position="12"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="4">
        <location>
            <begin position="444"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="825"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="927" mass="104611" checksum="96C12B714F2309A4" modified="2014-04-16" version="1">MDFRGKKYERGSNWSDPEVVELLQLWADESVQMELESCLRNQHVFNRIAEVLRDKGIHRTGDQCREKIKKMKLEYRRIKDNQKTVRGGRTWKFYEVMDRVLTNRPSISYGSLGGSVIAQQVLQGVPGVESGYHLHAFAPPPPGAFIFGPPPKAGELMEIKCEEVDSDDPCLTPDPPPEMLYPIGSGDEPEADRPLGLEREGSVGGGREDGLLEASISPSGNAEPGDPGRCRGLPGCLRSYKAGCAPGPAPLGRVSLVRQQECIHHACETVFKEAYYTSNRCIVKCKVGLNISSFALEKTLLSRARYLAGRDIFPAVRVRLDQRFSRDDQNSSIDHEYIDPIWLTVKTQSTHSTEYLRTWSIRDVVPHPVLLTIIHSCLTIGAQWVPAFLLIVENTKVTNSRYLSRRGNRIRGHDGILQEGYALYHADKHDEDENPHGIINFGVSENKLCFDLLSKRLTQSDMFQIDAPLLRYPDWKGHPFLREEVASFLSCYCTAPSPLSPDNVVVMNGCGSLFSCLAAVLCDHEDAILIPTPFYGVITEDVRLYSGVRMVHAHLDCQPRYSGDRPFQLTVEKLEKALEEARLEGINVRALILVNPHNPLGDIYSPSEMKEFLEFAKRHELHSIVDEVYMLSVFDDSVSFHSVLSFSKLPDPQRTHVLWGLSKDFSASGIRVGTLYSENREVVEALEKLASFHGVPGPTQHQVARLLSDRDWIDRVYLQANRTRLKTAHQYIVSELKALNVPFLNRPAGLYIWVDFRKYLREETFEEELTLWRQFLNNKVLVSCGKAFHCSTPGWFRIVFADKTHRLQLGMQRLRRTLEELDQGTVLEDSKAKPGTSGGRTKLAQSELRKRQSCPGSEVSLGRDADDDVQVSLQSGPPASGLDSLIGVLWQQIRTSDWLEKNTPESFAQENPEVFEVFSKLVERPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZG7</accession>
    <name>W5LZG7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="10">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="10">4.2.1.109</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">APAF1-interacting protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">APIP</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway. May play a role in apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01008112">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZG7"/>
    <dbReference type="FunCoup" id="W5LZG7">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001524"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001529.1">
        <property type="protein sequence ID" value="ENSLOCP00000001524.1"/>
        <property type="gene ID" value="ENSLOCG00000001341.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001680"/>
    <dbReference type="HOGENOM" id="CLU_006033_4_0_1"/>
    <dbReference type="InParanoid" id="W5LZG7"/>
    <dbReference type="OMA" id="WFPGTSG"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001341">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="11">
        <location>
            <begin position="25"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="242" mass="27261" checksum="409A43F7A2E06F22" modified="2014-04-16" version="1">MSSAVYNRDCSKCAQSIEDKDNPRLLIPELCKLFYQLGWVTGTGGGISLRQGDHIYIAPSGVQKERIQPEDMFVCDMEERDISCPPPTKSLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGKEFRITHQEMIKGIRKGSSGTNYSRYDETLVVPIIENTPEECDLKERMARAMEGYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVQMKQCGLNPSQLPIEEKGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M050</accession>
    <name>W5M050_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoinositide-3-kinase, regulatory subunit 2 (beta)</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3K p85 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M050"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001757"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001762.1">
        <property type="protein sequence ID" value="ENSLOCP00000001757.1"/>
        <property type="gene ID" value="ENSLOCG00000001519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687995"/>
    <dbReference type="CTD" id="5296"/>
    <dbReference type="eggNOG" id="KOG4637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157050"/>
    <dbReference type="HOGENOM" id="CLU_007031_1_0_1"/>
    <dbReference type="InParanoid" id="W5M050"/>
    <dbReference type="OMA" id="SERCPQN"/>
    <dbReference type="OrthoDB" id="3175255at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001519">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12926">
        <property type="entry name" value="iSH2_PIK3R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09930">
        <property type="entry name" value="SH2_cSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09942">
        <property type="entry name" value="SH2_nSH2_p85_like"/>
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    <dbReference type="FunFam" id="2.30.30.40:FF:000075">
        <property type="entry name" value="phosphatidylinositol 3-kinase regulatory subunit alpha"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
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    <dbReference type="FunFam" id="3.30.505.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
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    <dbReference type="FunFam" id="1.10.287.1490:FF:000001">
        <property type="entry name" value="Putative phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032498">
        <property type="entry name" value="PI3K_P85_iSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035020">
        <property type="entry name" value="PI3kinase_P85_cSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035022">
        <property type="entry name" value="PI3kinase_P85_nSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16454">
        <property type="entry name" value="PI3K_P85_iSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00678">
        <property type="entry name" value="PI3KINASEP85"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="113"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="9">
        <location>
            <begin position="336"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="9">
        <location>
            <begin position="628"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="729" mass="83478" checksum="825FFFB3B2125591" modified="2014-04-16" version="1">MSAEGFQYRALYVYNKDWDEDVDLQPGDLLTVNKASLLALGFKEGDEERPGELGWMVGVNERTKQRGDFPGTYVQYVGPVKMSLPLNQPRFQRPLPAAPRAEPPPVRQALSVLDLTEQFSPPESAPPVLVKLVEAIEKKGLDCETLYRTPGFSAAVDQRQTLNFAELPAGEMGQCDLQVLTEAVKRYLQDLPTPVIPPAVCSELQAATQQGTEAATAGSCKQLMLVLEQPAVPLQNLLTLQYLLRHLCNVCQNSERNGLDIHLLSQIFGPLVLRVPSMGFEGSSDFPVLVLERLLLERSWEQERPPPALPPKPPKTKALTNLVANGNSSLLADAEWYWGDISREEVNEKMRDTPDGTFLVRDASSKVQGEYTLTLRKGGNNKLIKIFHRGGMYGFSEPLTFLSVVELISHYRHESLAQYNAKLDTRLLYPVSKYQQEQVVKEDSIEAVGEQLRVYHEQYQEKSREYDVLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQCETQERYSKEYIEKFRREGNDKEIQRIQSNSEKLKSRVTEIHDSKRKLEQDLKKQATDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGTRQRRINEWLGITNEAEDLYSLMDEEDDLPHHDERMWYVGDVKRVQAEDLLRGKRDGTFLIRDSQSQKGSYACSVVVDGDIKHCVIYKTVTGYGFAEPYNLYGSLKELVLHYKHTSLVQHNDSLNVTLAWPVLSPQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2I5</accession>
    <name>W5M2I5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011223">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011224">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M2I5">
        <property type="interactions" value="1373"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002599.1">
        <property type="protein sequence ID" value="ENSLOCP00000002593.1"/>
        <property type="gene ID" value="ENSLOCG00000002230.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0898">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000475"/>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_1"/>
    <dbReference type="InParanoid" id="W5M2I5"/>
    <dbReference type="OMA" id="CARRRFQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002230">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="145" mass="16967" checksum="84767E302D489FBF" modified="2014-04-16" version="1">QADVEQKKKRTFRKFTYXXXXNHARVFLSSEQLMQLYCARQRRRLNRGLRRKQQSLLKRLRKAKKEAPPMEKPEVVKTHLRDMVILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2P8</accession>
    <name>W5M2P8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01033098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2P8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002662.1">
        <property type="protein sequence ID" value="ENSLOCP00000002656.1"/>
        <property type="gene ID" value="ENSLOCG00000002274.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SI0B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167701"/>
    <dbReference type="HOGENOM" id="CLU_192588_0_0_1"/>
    <dbReference type="InParanoid" id="W5M2P8"/>
    <dbReference type="OMA" id="QYASTME"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG28"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002274">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043441">
        <property type="entry name" value="Tjap1/BEGAIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28664:SF3">
        <property type="entry name" value="TIGHT JUNCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="85" mass="10088" checksum="0F2DDE098839F718" modified="2025-10-08" version="2">MQEQNEELHHSLVKTTVRMEVMGSELKSSHQQLETELQRTREELERLRENFRRSEDSYTSSLQTNVALEQKLRSVVSSSSLLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M342</accession>
    <name>W5M342_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Rab GTPase-binding effector protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Rabaptin-5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Rabaptin-5alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the rabaptin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005644">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M342"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002800"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002806.1">
        <property type="protein sequence ID" value="ENSLOCP00000002800.1"/>
        <property type="gene ID" value="ENSLOCG00000002386.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102685084"/>
    <dbReference type="CTD" id="9135"/>
    <dbReference type="eggNOG" id="KOG0993">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063743"/>
    <dbReference type="HOGENOM" id="CLU_016882_0_0_1"/>
    <dbReference type="InParanoid" id="W5M342"/>
    <dbReference type="OMA" id="XDLKRQN"/>
    <dbReference type="OrthoDB" id="79940at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002386">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000022">
        <property type="entry name" value="Rabaptin, RAB GTPase-binding effector protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.730:FF:000002">
        <property type="entry name" value="Rabaptin, RAB GTPase-binding effector protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003914">
        <property type="entry name" value="Rabaptin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018514">
        <property type="entry name" value="Rabaptin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015390">
        <property type="entry name" value="Rabaptin_Rab5-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179">
        <property type="entry name" value="RAB GTPASE-BINDING EFFECTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179:SF5">
        <property type="entry name" value="RAB GTPASE-BINDING EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09311">
        <property type="entry name" value="Rab5-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03528">
        <property type="entry name" value="Rabaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01432">
        <property type="entry name" value="RABAPTIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rabaptin coiled-coil" evidence="21">
        <location>
            <begin position="14"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Rabaptin GTPase-Rab5 binding" evidence="22">
        <location>
            <begin position="523"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="16"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="671"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF09311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="852" mass="97258" checksum="DEEF094515E58C2F" modified="2014-04-16" version="1">MAEQGPGSAPPPQHDVGELQRRLAELEQELKLKQQLEMEFNQKRAKFKELYLSKEEELKRQGASLETSQVELSRLQAKLADAQAEMENIKAVATVSESTKQEAIDQVKRQWQEEVASLQAIMKETVRDYEQQFRQRLEQERAQWAQYREAAEREMAELRRRLSEGQQEENLENEMKKAQEDAEKLRSVVMPMEREIAALKAKLTVAENRVKELEASKVMEMNHVLEAEKSCRTDLEMYVAVLNTQKSVLQEDAEKLRKELHEVCHLLELERQQHNQLKHTWQRANDQFLESQRLLMRDMQRIESVLSSEQLRQVEEMKKRDQEEDEQQMRAKGLKDEAGIEQAEPAEDSLVTLPNDESNINHSIHSSLHSLDMEPMTALDGSDLFKEGLRRAQSTDSLGSSGGSLQSRGLGCNHKAKSASNLDESDFGPLVGADCGGSENLDTSSLSSFQLSTGAFLLTKDQEKAIKAMTPEQEETASLLSSISQAPDTAYLPASGYRLVSESEWNLLQQEVKNAGRKLGRRCDMCSNYEKQLQVIQGQEAETRDQVKKLQVMLRQANDQLEKTMSEKQELEESVKQSSEETSAKVSTLMQKVQESEAVLSHLKQALVQAKRSTQDQMAVLTQSREQVAEELSRLQRDNESLQGKHRLHLSLQQQEDFRMPSSVQDLQALVLQYREDIVAARTAADHLEEKLKAEILFLKEQIQAEQCLKENMEETLQLEIEGCKEEIASFSSLKTELERVKTEKGQLESSLSEKTQQLEKLQGLRSKLEEQLKEANSTKAALESQVLDEKDKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADCLERVRAILNDTNLTDISQLPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3Y3</accession>
    <name>W5M3Y3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor AP-4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031922">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637097.1">
        <property type="nucleotide sequence ID" value="XM_006637034.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3Y3"/>
    <dbReference type="FunCoup" id="W5M3Y3">
        <property type="interactions" value="768"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003091"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003097.1">
        <property type="protein sequence ID" value="ENSLOCP00000003091.1"/>
        <property type="gene ID" value="ENSLOCG00000002626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695352"/>
    <dbReference type="KEGG" id="loc:102695352"/>
    <dbReference type="CTD" id="7023"/>
    <dbReference type="eggNOG" id="KOG0561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015189"/>
    <dbReference type="HOGENOM" id="CLU_064747_0_0_1"/>
    <dbReference type="InParanoid" id="W5M3Y3"/>
    <dbReference type="OMA" id="TQPHHVT"/>
    <dbReference type="OrthoDB" id="10029128at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002626">
        <property type="expression patterns" value="Expressed in zone of skin and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11419">
        <property type="entry name" value="bHLHzip_TFAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000036">
        <property type="entry name" value="Transcription factor AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
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    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="48"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="148"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="333" mass="37678" checksum="8A63E6C95227EB14" modified="2014-04-16" version="1">MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQEFSGSSPKRRRAEEKDEGIGSPDILEDEKVEDLRREMIELRQQLDKERSVRMMLEEQMRSLEAHMYPEKLKVIAQQVQEQEQSRLRLQQQHDRLEREQQSLRGGGAQVVPPATTPAPTHHPTVIVPAPALPPLPHHVTVVTMGPASVINTVSTSRQNLDTIVQAIQHIEGTQEKAGTDEEQRRAVIVTPGRTFPDTPDSDTASDSEGGAGDGELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3Z6</accession>
    <name>W5M3Z6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sarcalumenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Sarcoplasmic reticulum membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01031919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3Z6"/>
    <dbReference type="FunCoup" id="W5M3Z6">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003104"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003111.1">
        <property type="protein sequence ID" value="ENSLOCP00000003104.1"/>
        <property type="gene ID" value="ENSLOCG00000002644.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157834"/>
    <dbReference type="HOGENOM" id="CLU_017595_4_0_1"/>
    <dbReference type="InParanoid" id="W5M3Z6"/>
    <dbReference type="OMA" id="PIMSHER"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002644">
        <property type="expression patterns" value="Expressed in larva and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000635">
        <property type="entry name" value="Sarcalumenin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="11">
        <location>
            <begin position="321"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="704" mass="78968" checksum="D0F0AF7FAA8B9F47" modified="2014-04-16" version="1">KTEQSLEDGGSDTSHEDIAESGAKGAEGDTFEDESSADKSQDFEGYTDADGVESESRPQREDVEKTETSEAPVASLESEEELVEDYEELGYQDEEDLQGSLEEEKGGGHQGEQEEDAENLNVEKIELDKTEELNQGDEAASFSSEQNKMEDNEGDREGLDSGQENSVSSSESQESNSPGSEGYQDQEGTEAVEGASDKGPGEDLGSSGGTEEPAEFKANETHGDELDTTEGGERSGEGEELQENTAKEVEDVLVPVLRDRSHIEQTLRLKEDAPSNDYSGQKSDRTALQRLRKIYHSSIKPLEQAYRYNELRQHEISDGEITSKPMVLFLGPWSVGKSSMINYLLGLQETPYQLYTGAEPTTSEFTVIMHGEKIRSVEGIVMAADSSRSFSPLEKFGQNFLEKLVGIEMPHKLLERVTFVDTPGIIENRKQQERGYPFNDVCQWFIDRADLIFVVFDPTKLDVGLELEMLFRQLKGRESQIRIILNKADSLATQDLMRVYGALFWSLAPLINVTEPPRVYVSSFWPYDYAQDTSRELFKREEISLLEDLNQVIENRLENKIAFIRQHAIRVRIHALLVDRYVQTFRDKMTFFSDPELVFKEIVDDPDKFFIFKSILAKTNVSKFDLPNRDAYRDFFGINPVGGFKQLSAQCSYMGGCLLDKIEKAITHELPGLLGGIASGKNPSLTSCEATGCGETPKNRYRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5M453</accession>
    <name>W5M453_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Si:rp71-39b20.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01011202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M453"/>
    <dbReference type="FunCoup" id="W5M453">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003161"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003169.1">
        <property type="protein sequence ID" value="ENSLOCP00000003161.1"/>
        <property type="gene ID" value="ENSLOCG00000002693.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164521"/>
    <dbReference type="HOGENOM" id="CLU_011722_4_1_1"/>
    <dbReference type="InParanoid" id="W5M453"/>
    <dbReference type="OMA" id="DAPNPWQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002693">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000042">
        <property type="entry name" value="Potassium voltage-gated channel subfamily V member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000188">
        <property type="entry name" value="Si:rp71-39b20.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF65">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="15">
        <location>
            <begin position="84"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="245"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="538" mass="62352" checksum="3A6C2EE69EE8089D" modified="2014-04-16" version="1">ADLRRNVMKKLKKRRSSLFANLKFGDSFSQSTDDDESAFPFAMDSSVKPWTSLHNLDKPLDSDGKVGEDDWEQCWSPPGKMSMINLNVGGKVFQIAKTVAVRYPHTRIGCLALCSDPVKKLELCDDYSVLNNEYFFDRDPTFFHYIFHFYRSGVLWVMQELCPINFEEEMEYWGLRMKDTQRCCRILFEEKVDELKDYLKVQRELKAEIEPEHRDEHFEGMFLGGFRRALWNLMETPYSSVWAKGFAIISSIFVLISIMAMTLNTVEELKSRTLHGKTYMECVEIFCIIFFTLEYFLRLLTTCDVKRFLRSALNFVDLVAVMPYFVQVVFETFADHSDDSYQNDLQAMQRVSKVSKVLKVIRLMRIFRILKLARHSTGLRAFGFTIRQCYQQVCCLFLFIGMGIFSFSALMHSVEHDVPGTPFSSIPDAWWWAAVSISTVGYGDTVPESFLGRLVAFGCISFGIILNGMPISILFNKFSDYYAKLKDQEVANTMKQRGRIQLRVRLRRKMDKCLHSQAPDCDEDSLGRLDPRAWHGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4D6</accession>
    <name>W5M4D6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uroplakin-1b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Uroplakin Ib</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer with uroplakin-3A (UPK3A) or uroplakin-3B (UPK3B).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003268">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628039.1">
        <property type="nucleotide sequence ID" value="XM_006627976.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M4D6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003244"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003251.1">
        <property type="protein sequence ID" value="ENSLOCP00000003244.1"/>
        <property type="gene ID" value="ENSLOCG00000002757.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102692189"/>
    <dbReference type="KEGG" id="loc:102692189"/>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160779"/>
    <dbReference type="HOGENOM" id="CLU_088971_1_0_1"/>
    <dbReference type="InParanoid" id="W5M4D6"/>
    <dbReference type="OMA" id="EKCCGVN"/>
    <dbReference type="OrthoDB" id="5982705at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002757">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03156">
        <property type="entry name" value="uroplakin_I_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000014">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47110">
        <property type="entry name" value="TESTIS-SPECIFIC EXPRESSED PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47110:SF2">
        <property type="entry name" value="UROPLAKIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="265" mass="30139" checksum="1AB9AA1845A308C7" modified="2014-04-16" version="1">MVAKSDIGVRCFQAFLAWGNFVIACCGLALMAMCIFFIFDRDHLYVLVYATGNDSIWRAAWIGLFTGFALFCTAVFGMYAVLKSKRGLLLVYILLMIFIFAFEVASAITAATHKDWFVPNLFLKQMLENYNKPLPQNLPSTQDEIYKNSGITSTWNMIMTEYQCCGVYGPQDWLQYNSQFRQQNSDSEYPWPRQCCLQDGTGGIVNVNACKIGVEPYLFTQGCYDYIAGPLIRQGLGVSWFGFAILCWTFFVVIGVMFYYTQLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5H9</accession>
    <name>W5M5H9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribitol-5-phosphate transferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LicD transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01012591">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5H9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003637"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003644.1">
        <property type="protein sequence ID" value="ENSLOCP00000003637.1"/>
        <property type="gene ID" value="ENSLOCG00000003089.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QUDN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014471"/>
    <dbReference type="InParanoid" id="W5M5H9"/>
    <dbReference type="OMA" id="ICKWATH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003089">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045587">
        <property type="entry name" value="FKTN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19737">
        <property type="entry name" value="FKTN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Ribitol-5-phosphate transferase FKTN N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="8">
        <location>
            <begin position="290"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="459" mass="52906" checksum="8642EB5E7D185360" modified="2014-04-16" version="1">MPRINKSVVLALLTVTSSVFLLFQLYYYKLYLSKNGSHYFKVKDGLPASDAQWWHVTKKFMELVAKHNLPVYLIDPVVLGLLSQDAHPLKDSLSKGKDCRYICTQREFITFALLAKFWKNDARLAHAAAEAGLEFLEVRGKDPRLAGPDDLSGMEIPLHLLFRLRAHAVHLLVFYERSGGYLWHGPLRLRPGADRKFVPFRKLDFGRHAGAFDSRTELLLTSVDGLNIRIPKNISRFLNEVSHARFLECRYKEARAFYQLYPDDTSQEAMDFRQKAKSLLHLAARTLNALGVTFWLSSGTCLGWFRQCNIIPYSKDVDLGIWIKDYRPDIIPAFQKAGLPLKHKFGKVEDSLELSFQGQDVKLDIFFFYEDSDIVWNGGTQAKSGKKFKYVFPKFSLCWTELVELKVQVPCETLEYIEANYGKSWNIPVKTWDWKSSPSNVQENGQWPVAEWDEVIQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5L3</accession>
    <name>W5M5L3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch domain containing 7A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M5L3"/>
    <dbReference type="FunCoup" id="W5M5L3">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003671"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003678.1">
        <property type="protein sequence ID" value="ENSLOCP00000003671.1"/>
        <property type="gene ID" value="ENSLOCG00000003121.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162724"/>
    <dbReference type="InParanoid" id="W5M5L3"/>
    <dbReference type="OMA" id="PLTSIWK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003121">
        <property type="expression patterns" value="Expressed in zone of skin and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052310">
        <property type="entry name" value="Kelch/BTB_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972">
        <property type="entry name" value="BTB_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="707" mass="79108" checksum="6AAC24E78CA75843" modified="2014-04-16" version="1">MPIADLLGVQFDMQLLGKLSLSVAAVLLISWAYKFYSSRGFTVHQDGSAQGNGKMVQQSHRDVSSGSCISCNSELRPRQKNLAEDAEEKSLVANKPDIESKPRDTSPIKDPADESVIDKHSSALLSTGTNKWKLEPGSGVGRELKQNLEHPGSYFSFQSKAEITVGDGDLLLGRPGGELMEVRGKIYDYYVESASQSISGDSAYHSTFLSPARRSCEDNVLQDSSFTLFPQDSELCYKDSRSPLSPVGLLPQSSGRQGHGLSRKESISQIVENSDLQIPFMEARASTPVNSEFDSRTDSSEDLCSSPDSLMFPEPSLEVIAGANFLQMPQDSIGTFELEGLKCKLDLGNCLQALSLARKYKRKDLQQAAFKVMSDNYFQVLRDPELYGRLKADERDQIQRLRMKGRRYLIVADMDPQDLMRPKLSKAETSRTSSMLYYYDDYKDTWHPLSSIPKEVISKGCAMCTMDNYLFIAVGCNGLERDLKPSKNVFCYNPVTGIWKEISPMNEARPHCKLVALQGYLYAIGGECLYTVERYDPRMDRWTFVAPLPNDTFAVAHKATACNGQLFVSGGTLRYSLLRYNPKTNSWKESLIVGTKERATEMVAIRNFIYRFDVNSSLGISVYRYHAMARLWYECCSKRLPCCAAFQCATIDDVIYCVNRQFTMRFLADEVSPAFVADDLKVLPMAKGVLFPFVLVLPEKGTLQTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6K5</accession>
    <name>W5M6K5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Olfactomedin-4-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01010563">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M6K5"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004021.1">
        <property type="protein sequence ID" value="ENSLOCP00000004013.1"/>
        <property type="gene ID" value="ENSLOCG00000003376.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3545">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165415"/>
    <dbReference type="HOGENOM" id="CLU_035236_5_0_1"/>
    <dbReference type="InParanoid" id="W5M6K5"/>
    <dbReference type="OMA" id="NFCHLED"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003376">
        <property type="expression patterns" value="Expressed in liver and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF68">
        <property type="entry name" value="OLFACTOMEDIN-4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867692" evidence="4">
        <location>
            <begin position="26"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="5">
        <location>
            <begin position="194"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="474" mass="53411" checksum="2D136F1C83D26054" modified="2014-04-16" version="1">MKPSFIFSAVTAVVLCSLLKSWVSAQVSKACFCELQNLGQAFPSDKLEEILRTSTACTRSATEEQHELTEVETLLDSLHYRLLDLEKDVKDLEEAKDEGMYGIISLRIIEIELNEISDLLIRLNSTTRNHQALTVNLGPQLRNLRTELGKLADFDRMQVVKKEQENKRLKRHLSVCETDLRMTPAPHKDPAPGNCPFGRLLNVTGPTSSMLTEYDSSYPYGAWGKDPKPAPGKESWYWLVVLTSSSCYGSFVRTYTSRSAQIVAQYAGDVIVTSSVTTTNSIMGPGSVMYNNAYYYNCYYSPNVCRFDMETKTITTRALPGAGANGKFPYCHLTACYSCTDIDLSTDESGLWVIYASEENFGNVVLSRLNATSTLDILETWRTSLVKKAVTNAFVACGVLYATRLVNNNYEEIFYSFDTVTGIERGNLSIRFRKPSAYIYSLNYNPRDRNLYAYNDAYIVTYQTVFGLWPHFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6P3</accession>
    <name>W5M6P3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat domain 90</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01029991">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5M6P3">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004051"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004059.1">
        <property type="protein sequence ID" value="ENSLOCP00000004051.1"/>
        <property type="gene ID" value="ENSLOCG00000003416.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160173"/>
    <dbReference type="HOGENOM" id="CLU_002454_0_0_1"/>
    <dbReference type="InParanoid" id="W5M6P3"/>
    <dbReference type="OMA" id="DHYVHIR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003416">
        <property type="expression patterns" value="Expressed in testis and 10 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000590">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001417">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055440">
        <property type="entry name" value="Beta-prop_WDR90_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055441">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
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    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
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    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF24">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23393">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23342">
        <property type="entry name" value="WDR90_beta-prop_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CFA20" evidence="4">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="CFA20" evidence="4">
        <location>
            <begin position="132"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90/POC16 second beta-propeller" evidence="6">
        <location>
            <begin position="714"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90 4th beta-propeller" evidence="5">
        <location>
            <begin position="1481"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1262"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1661" mass="184271" checksum="215A07C78F4AB08A" modified="2014-04-16" version="1">MSTKVWQHPYVNIFKHVRVEDWKKCSREGDVTTYMDKTLKCSVYRIRGSIPASNYILLPKTSTQSLGLTGRYLYLLFRPSPGKHFVVHLDVATEDGQVIRISFSNLFKEFKSTATWLQFPFICGAAKGSVYESTAQTARQGLVGPAPPSVRWTCLMMDLHYVLSIYLNRRHSHLKSIKLCANMSVKNMFTSDLLCDPGLTFSEARQAGVEALQGASPMPREMSFPVPKGGSWHDLYDYIRFPSDGTKMPFDSIQKGHVLRAAVGAPVLPSPVRELARSVNVSKPVQDRVSLIQQITTPKPVSVPELGRVSSVPVGEELRESWAGGGRTTEQGAEGDSGTPLASDDGAVHVFAHRDSDITVPGHDSDTEETVCTKVTRPVSLQSKKVLERTQNLHPDPILKLNRIIGFGGCTTKCALWTKTGEAVVYPCHAVIVSMKIVSGQQRFFIGHTDKVSALAFNGNSTLLASAQTGSLSAVRVWGYQKGSCLAMFKTHAHSVSSLSFSHSGGVLCGVGKDSHGKTMVVVWNTQKVQRGGEVVVMAKAHTDVDIQAMKIAFFDDTRMVSCGRDNIRLWRVRSGALRSCPVNLGEYHCMEFTDIVFEEGHLADREPEDRTLFASSKSGHILEIDYKTVTIKNVRRLLPSQQKHSLHREKQTFSSGPGIAVNSISISSAFCATGSEDGYLRLWPLDFSTVFLEAGKKLQWPISSPLGVRVAMCLQLYDFISEDTPCAVSFHPTQQEFACGFRSGLLRVFNIGTSSLLAEHKQHRGEVIGLAFSPDGEYMYSAGSLGSLALYNSAEKDHHVLRVLGNVVSRGSTRCPDALAVSSDSRSLAFVGPSEHTVTVMDARSLDELMRIDVSVLDLDSTSLDSAVKVCFPPAPTGHLLVTTSANKILWLNAKTGRLLREVSNVHKHVCSSLSVSEDGRFLLTAGHKAVKVWDYRMKHDTSYQVFIGHSQPIQQVGFTPDQLGVVSVGDAIYYWDFLAYPEEMEEDKRLASTLKDVISRVTTVPFTTCNISFLSDSLPLLPLTHLSNQVRLCFQNNDHIIKKIRYLNNILLGDSVQKSLHAKKRIHFYTVPAAELMKPQCFEIAKCESGFHGDCLFQCLSSCSPGLFAYSCGCVVVVEDLHSGSQRHLLGHSEEISTLAVTHDAQVIASASGGSDGSRSLICIWNVPDGSRRNTISYHRGHVQAMAYSRDDFFFLSAGQFRSLSQAQRIFQTFLQAPPRTHLKLRKVGLLLIIKVTPLNSISEICVSKRRPLVIRRQTKDPHNQCGNVPPPSHRSSGKDGLMAVSSPRTGMTIRIISDHKGALISTIQCVKKQVRKCRLMSLHLGPESVSAMLSEILHSKAIQVIILTLGIILKLLYESKTTLIKSLCKYFSLCILYSLSNPFHKLRVLDHVLINYVVISVVSLYVFFHLKLMPFQAFWTQFPLILLTESFVQHVSKSPFTANLLCSVWSLLYKIESVLTLYTLYQRQRHCVFEWRDGLHLAVLRNNRLVLNEFGLEGNELWLAASADRRVSIWASDWLKDKCELLDWLTFPAPARTEELDSLPPSLAAFCPSAPGVVLYAGYGVEKEIVFYSLLQKQIIKRIALSHWATCLSLSPKGHLIAVGSDERLLKLIKSTSGKFQDFVAHSDAVHMCRFAPSGNRLFTSAYNEILLWDVLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M6R3</accession>
    <name>W5M6R3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit 36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012412"/>
    <dbReference type="HOGENOM" id="CLU_053706_0_0_1"/>
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        <property type="component" value="Linkage group LG11"/>
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        <property type="expression patterns" value="Expressed in brain and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Pro_pase_1_reg_su_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14895">
        <property type="entry name" value="PPPI_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48367" checksum="033E3477BC2FEA54" modified="2014-04-16" version="1">FQQVSISTPGRWHWNDELQTLELFRFFFSFNSTIETKDKRKKNKSIHFQEQANKRVERGNTGNDICLLSRTVDKKYNGKAQLGTCKPSLKHGPHDHVTIEDVKNVAFSLLQENEPIPLCFMSLMRCQQVNDFLSALLLYLSCYFEKQALEKKTQPLMAEPSLTERKNMEEAHIKVELAKKQLACAYSVLVLGLGMSQQHHMDCGRSRVSATYKDRQLFECLYSFSTYVAWVTFRRKDLKGIQEEVGRLLRSDTFNHAVRKTEDNNEDARSDTTLNEEIGSTRERRKMKSKRPAIKSIVKQRSPVITSLLPSPKEKSQHLFKTGCLEQDCSTDPCGTKSLMEELPAALSSNLGIIGQPLSQFSRHTLVPLGAEQEEEEEDDEDHARNSKASFRSKGPLSSGGGKYAGLSRANTVISRATTVGVYSDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M798</accession>
    <name>W5M798_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Stathmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the stathmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009526">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631326.1">
        <property type="nucleotide sequence ID" value="XM_006631263.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M798"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004256"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004264.1">
        <property type="protein sequence ID" value="ENSLOCP00000004256.1"/>
        <property type="gene ID" value="ENSLOCG00000003586.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688714"/>
    <dbReference type="KEGG" id="loc:102688714"/>
    <dbReference type="CTD" id="550548"/>
    <dbReference type="eggNOG" id="KOG1280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="26"/>
            <end position="48"/>
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        <location>
            <begin position="118"/>
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            <begin position="121"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023212"/>
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            <dbReference type="RuleBase" id="RU004388"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSLOCP00000004256.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="147" mass="16809" checksum="1C8C35281D2B58DB" modified="2014-04-16" version="1">MASSGADIQVKELDKRASGQAFEVILSPSAPEPKADFHLSPPKKKDVSLEEIQRKLEAAEERRKSHEAEVLKHLAEKREHEKEVLQKAMEENNNFSKMAEEKLNQKMEANKENRTALLAAKSEKFKEKDKKIEEVRKNKETKEGGEN</sequence>
</entry>
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    <name>W5M7F7_LEPOC</name>
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        <submittedName>
            <fullName evidence="2">HAUS augmin-like complex, subunit 5</fullName>
        </submittedName>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="74"/>
            <end position="108"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="651" mass="74078" checksum="0BF24AE15D8E15A9" modified="2014-04-16" version="1">MGDKDLPQVLKRWIVEELELPARDLPPDSYFRTLCLGQGADIWKYITRHVYNEGLTRSCNLPACWSYISPPVDGDSAVARRRALTREIEELRAELVELDAQIEQSQAELCSDDRAVGQCWDHCSEARRRRVLLQAMGVRCAQERHSLWQDRRRISGHCQALAELARKAEVELVFGDPSDSSTDSLGSTGPEPQALREVRELCEDRLQFFWTLLESELKAAPLSGVHLSQEERSAVFQRWLSSVEDLMRTHPPSHLLAALQHLASRQQGALQEKLEQLDLSRDVAALGFRYESDHLLDLSKETAAPLPSVRLLLQEAWETVERCQVQLAAMRAQDRQQSALLQARKSEAFQAACAGDAQVEPLLRAALELELQLAMETALRDSLQRQGEALEQGARSRKEALLALQAKRQNIMDFRQLVDRKQEQIRSLIKGNSTAKSELSRVHAEVRRFVQEKLQRHQGAVEREAGALHSAVSQEVRQAAGVSLDELDRRLLDGVQRVPARKLSVHRLDCPALRRVSDGLGFALYKAPEQLMPLAVSMQLELRFLRRLLQLHTTCMQHRDQQVALLPAQDMEAMLQRVRTEDQARVRDLLPRVRLLSQRSAQCLGYGAEVKRAISHWWEQPGQFVLPDQRRGGLTLQQWLERWRLAAKGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M839</accession>
    <name>W5M839_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Voltage-gated potassium channel subunit beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">K(+) channel subunit beta-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Kv-beta-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a secondary alcohol + NADP(+) = a ketone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:19259"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01009734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009738">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009739">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009740">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01009743">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M839"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004547"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004555.1">
        <property type="protein sequence ID" value="ENSLOCP00000004547.1"/>
        <property type="gene ID" value="ENSLOCG00000003792.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157867"/>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_1"/>
    <dbReference type="InParanoid" id="W5M839"/>
    <dbReference type="OMA" id="FEWEYES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003792">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000001">
        <property type="entry name" value="voltage-gated potassium channel subunit beta-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005400">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF1">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01578">
        <property type="entry name" value="KCNAB1CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="18">
        <location>
            <begin position="85"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="415" mass="46580" checksum="B7D92A9D47E69527" modified="2014-04-16" version="1">MLSMSYSESVRSGINRYHSDLGLNQPRHTDQAELQRLREVRAAAQVKNMEDFLKMNGLSLEDCVAYQTGMKYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEQLMTLAYENGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEGRHLKAFIMFRVKILPAVSEFTSASTLTVSGFFNQRYYIIYLLSEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIISGKYDSGVPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNTDQLMENIGAIQVLPKLSSSIVHEVDSILGNKPYSKKDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8N3</accession>
    <name>W5M8N3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MOB-like protein phocein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mob1 homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Mps one binder kinase activator-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Preimplantation protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">Golgi stack membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOB1/phocein family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011488">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011489">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011490">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006636351.1">
        <property type="nucleotide sequence ID" value="XM_006636288.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8N3"/>
    <dbReference type="FunCoup" id="W5M8N3">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000004741"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004749.1">
        <property type="protein sequence ID" value="ENSLOCP00000004741.1"/>
        <property type="gene ID" value="ENSLOCG00000003970.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696336"/>
    <dbReference type="KEGG" id="loc:102696336"/>
    <dbReference type="CTD" id="25843"/>
    <dbReference type="eggNOG" id="KOG1852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271909"/>
    <dbReference type="HOGENOM" id="CLU_056981_3_0_1"/>
    <dbReference type="InParanoid" id="W5M8N3"/>
    <dbReference type="OMA" id="ATCTQMT"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000003970">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000002">
        <property type="entry name" value="MOB-like protein phocein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="225" mass="25988" checksum="2862F118853DB148" modified="2014-04-16" version="1">MVMAEGAAVLRRNRPGTKAQDFYNWPDESFEEMDSTLAVQQYIQQNIRSDCSNIDKILEPPEGQDEGVWKYEHLRQFCLELNGLAVKLQSECHPDTCTQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKESSVAKLGSVCRRIYRIFSHAYFHHRQIFDKYENETFLCHRFTRFVMKYNLMSKDNLIVPILEEEVQSAASGESEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5M8X9</accession>
    <name>W5M8X9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase/reductase 12-like a</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01029696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M8X9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000004838"/>
    <dbReference type="Ensembl" id="ENSLOCT00000004846.1">
        <property type="protein sequence ID" value="ENSLOCP00000004838.1"/>
        <property type="gene ID" value="ENSLOCG00000004049.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165979"/>
    <dbReference type="HOGENOM" id="CLU_010194_44_8_1"/>
    <dbReference type="InParanoid" id="W5M8X9"/>
    <dbReference type="OMA" id="QFAKAHT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004049">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052992">
        <property type="entry name" value="SDR_member_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656:SF6">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 12-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000004838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="325" mass="36281" checksum="86B7CAB7A81BD625" modified="2014-04-16" version="1">VERVAMSVYRNTIWFLKGIHEYTRGGYEAASKHFVEKDLDVSVSGKSFMITGANSGIGKAAAYMIAKKGGTVHLVCRNKERAEDAKADIIKESGNKDIHVHILDLSEPRKVWEFAEAFKKKHKSLNVLINNAGCMVNQREVNAEGLEMNFACNTLGTFILTKSLIPLLEKSSNPRVITVSSGGMLVQKLHLDDLQSEQGRFNGTMVYAQNKRQQVVMTEEWAKVHSNIHFSVMHPGWADTPAVQSAMPDFYRSMKDKLRTADQGADTVVWLAVSEAALAHPSGLFFQDRSPVPTHLPLAWTKTSPEDKQKFINILDDLAKTFQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MAZ6</accession>
    <name>W5MAZ6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Minichromosome maintenance domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in meiotic recombination and associated DNA double-strand break repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004373">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MAZ6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000005555"/>
    <dbReference type="Ensembl" id="ENSLOCT00000005563.1">
        <property type="protein sequence ID" value="ENSLOCP00000005555.1"/>
        <property type="gene ID" value="ENSLOCG00000004629.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286951"/>
    <dbReference type="HOGENOM" id="CLU_032817_1_0_1"/>
    <dbReference type="InParanoid" id="W5MAZ6"/>
    <dbReference type="OMA" id="SICLVPR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000004629">
        <property type="expression patterns" value="Expressed in testis and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001155">
        <property type="entry name" value="minichromosome maintenance domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058769">
        <property type="entry name" value="MCMDC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF75">
        <property type="entry name" value="MINICHROMOSOME MAINTENANCE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26063">
        <property type="entry name" value="MCMDC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCMDC2 N-terminal" evidence="11">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="10">
        <location>
            <begin position="328"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF26063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000005555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="684" mass="74890" checksum="59E550A3B1E445A6" modified="2014-04-16" version="1">AQETLELKEAALSYMDRTGGLQRLIEDCRQYTDSQQSESVYRFCVRVNPTDVTEIDARLGNCILHDPLKAAGLFQSVCFISIKTLSLIEKICSENQVIVVLKLTHVPPFPDYLLDLSHVPRGDSPMRPLMLEGLAMAMTLVTKYTQGARFLCTEDSCPNSQGFQKIRVHVPGATEWATVRNDFTCSLCGSPLKEDVKSRVLGGQDKQLVEVIHAKALRVLSAHTLSSSRHQSLTVFLRDELCNSIKIGRLYRIIGIPAHVHQWPNVTFSVEANSVQPWVPECPSFISDNFQALLSATACSPWRFPAIVANAFGSQIVPPGLYNTLKLCLLLSLVQTAGEDRETLSYLDVLALTTDTLVIDRLMTYSLALASRGVRHRTSGELFPSVSRDEHGTGAASIHAGAALLATGGICFIGDLASCRKDKAELLQSALETRAVTVFIPGKKYGEDADQQLSLPLQCNIWALADTGASTKKFLKYENAALGSMDTGFLPSQMSDAFGLVIHCRELPIERPALPLAVHILRQAVKPEEPLYPASEQFTTDDYKGLIAAARGVQAELSPEAERLIHGYFLASRRVRTGTAQGPKVPVASVKLLFSFAEAHAKLSLRSRVLEEDAVIAVLLCENSITLKHGASALVLPPEAVFSCGLHDQASLERRDLALRQLHQQILQFVLTYAPGASSYISEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCD6</accession>
    <name>W5MCD6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">acetyl-CoA carboxylase</fullName>
            <ecNumber evidence="4">6.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01005772">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01005773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222854.1">
        <property type="nucleotide sequence ID" value="XM_015367368.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222855.1">
        <property type="nucleotide sequence ID" value="XM_015367369.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222856.1">
        <property type="nucleotide sequence ID" value="XM_015367370.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069040454.1">
        <property type="nucleotide sequence ID" value="XM_069184353.1"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006045"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006053.1">
        <property type="protein sequence ID" value="ENSLOCP00000006045.1"/>
        <property type="gene ID" value="ENSLOCG00000005003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686789"/>
    <dbReference type="CTD" id="31"/>
    <dbReference type="eggNOG" id="KOG0368">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156706"/>
    <dbReference type="HOGENOM" id="CLU_000395_5_0_1"/>
    <dbReference type="InParanoid" id="W5MCD6"/>
    <dbReference type="OMA" id="PTPKGHC"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005003">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF5">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="22">
        <location>
            <begin position="117"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="21">
        <location>
            <begin position="275"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="20">
        <location>
            <begin position="745"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="23">
        <location>
            <begin position="1585"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="24">
        <location>
            <begin position="1927"/>
            <end position="2243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="2356" mass="265846" checksum="A3885A29F7F201CF" modified="2014-04-16" version="1">MAEQDHPAGEPPEAPAALHSHFIIGSVSEENSEDEILGKVDLLGDKERSLSPSSVSSDSQYELGFDHLDGPLHHMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWSDSDQKKRNIKVPPELYEQGCIHDMEEGLQAAEKVGYPVMVKASEGGGGKGIRKVNSADDFPNLFRQVQTEVPGSPIFVMRLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATCAVFENMEKCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLQRIKDIRMLYGLQPWGDSPIDFEGLSTAPSPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKVQAERPDTMLGIVCGALHVADISLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEATKYALKVTRQSPNSYVVIMNHSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSLLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRAGAVLDPGCVIAKLQLDDPSRVQQAELHTGALPQIESLALRGEKLHRVFHSTLDHLVNIMNGFCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQNGHYDKCVFMLREENKGDMANVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELMSILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDSTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMLHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQDFMRNINDVMSCFSDSPPPSPTFPEGGHPALYGEEDSKSVRDEPIHILNVAIKTDGDIDDDGLAAMFRDFTQSKKSLLFEHGIRRLTFLVAQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGIEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGPKDQKIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQSLLKLWESTEAYAHLPKCPLPSELLTYTELVLDAQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPTGRDIIVISNDITHKIGSFGPQEDVLFQQASELSRAEGIPRIYIAANSGARIGLAEEIRHMFHVAWQDLSDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEEVITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHCTVCDDFEGVYTLLQWLSYMPKCNSSPVPILDAKDPIDRPVEFVPTKAPYDPRWMLAGRPSQSPKGQWLSGFFDHGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSVPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDLNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPIYMSLAERLGTPELSVVERKELEAKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWRTSRQFFYWRLRRLLLEDTVKRKIQAANSELTDGQIQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLKEEEGARSIIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMEAPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5MCE9</accession>
    <name>W5MCE9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Holocarboxylase synthetase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003113">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MCE9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006058"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006066.1">
        <property type="protein sequence ID" value="ENSLOCP00000006058.1"/>
        <property type="gene ID" value="ENSLOCG00000005030.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690116"/>
    <dbReference type="KEGG" id="loc:102690116"/>
    <dbReference type="CTD" id="3141"/>
    <dbReference type="eggNOG" id="KOG1536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002960"/>
    <dbReference type="InParanoid" id="W5MCE9"/>
    <dbReference type="OMA" id="VEHCPLH"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005030">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="658"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="323"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="930" mass="102615" checksum="6B07C082C38F50BB" modified="2014-04-16" version="1">MLITLCYVYLWVRFQRCYTVVIRSTVRRLCGGSRGFAFRTLEPGQWQKSQPAAEKSFQAHSPSLREDKVFLRLGDKVFCITEPQPCDDLSKWTVLLGSPLVYLDSVSRAKNIAFVIEASSDQELALKSLPTSRRKILKWSDHCLPLARSPGQPFKAIAEASVEDFSQIGVAFMEDRLQMDNGLVPDNIVSVLLQESALRELVEKRQMATDKQSQPSRPQESPGMGPVAEAASGHGGDRGGAVQQRSQPDGDGQQSSETVQGEKGETGGREQEEEEAEMDKEEANGVKDHHKEGHHLHLSSCHECLELENCTIESVKFASAENIPDLPDDYMGSASREEEEEDERSCGQSRRASVTGKPPNVLVYAGQEPAESEARYRRVRGLLAECLDADSYVVYQLRHEQVLRQPWLESARLVVLASDEPLAPALRQRFLSYVSLGGRLLGLSSPFCFGGLAVVPKAALRNRVHRLRFGRAGEPGPEPELELSVMASGGMFVREGDAEMDGQVELWGQLSGQDKDVVVVRLARGDRGGRAVLCQVRLETSPDSGDVQNPEDFDELKVSNARRYEAMSKILSSLGLSCEPSQVPPPSPIHLLSSSQDSRDRFLKWLWTKVDREGVLKSPKVSLKVASTAAPQTEQTEGLITLVTEPSEPEPSEQFCFHTYARNLQTGMLGHLVLYAEVTPTTMDLLEGLMLQLPQEMGLIAIAARQTQGKGRGGNAWLSPVGCAMFTLHVRVPVSSALGQRIPFLQHLAALAVVEAVRTLPGYEDIDLRVKWPNDIYYSDLMKLGGVLVTSTLMGSTFHALVGCGFNVRNSNPTICVNDLVVQHNKERGTALALLDTAQLIGRSVTLLEQLIQTFQDQGPQGVLPLYYRRWVHSGTQVRLWREDGPQVAVVGLDDNGFLQVASPDRRVVSVQPDGNSFDMLRNLVVAKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MDY4</accession>
    <name>W5MDY4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lens intrinsic membrane protein 2.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006642336.1">
        <property type="nucleotide sequence ID" value="XM_006642273.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MDY4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006593"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006601.1">
        <property type="protein sequence ID" value="ENSLOCP00000006593.1"/>
        <property type="gene ID" value="ENSLOCG00000005467.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102697981"/>
    <dbReference type="KEGG" id="loc:102697981"/>
    <dbReference type="CTD" id="573354"/>
    <dbReference type="eggNOG" id="ENOG502QSWZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244814"/>
    <dbReference type="HOGENOM" id="CLU_113769_0_0_1"/>
    <dbReference type="InParanoid" id="W5MDY4"/>
    <dbReference type="OMA" id="NFMHQGL"/>
    <dbReference type="OrthoDB" id="6137544at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG26"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005467">
        <property type="expression patterns" value="Expressed in camera-type eye and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000011">
        <property type="entry name" value="lens fiber membrane intrinsic protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003935">
        <property type="entry name" value="LMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF25">
        <property type="entry name" value="LENS INTRINSIC MEMBRANE PROTEIN 2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01457">
        <property type="entry name" value="LENSMEMPROT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01222">
        <property type="entry name" value="PMP22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="173" mass="19891" checksum="2082D7E00541265C" modified="2014-04-16" version="1">MYSFMGGGLFCAGVGNILLIVSTATDYWMQYRHSGNYMHQGLWRYCVPGKCYSHTESIAYWDATRAFMILSLLACFIGIIIGIMAFVHYSSFDRFDKTFAAGILFFISCLFVLLAMAVYTGVTVNYFGKRYGNWRFSWSYIIGWVAVVLTFFSGIFYMCAYRMHECPRSSHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEH2</accession>
    <name>W5MEH2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">PHD finger protein 21Aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01036566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036567">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01036568">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015194093.1">
        <property type="nucleotide sequence ID" value="XM_015338607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEH2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000006781"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006789.1">
        <property type="protein sequence ID" value="ENSLOCP00000006781.1"/>
        <property type="gene ID" value="ENSLOCG00000005618.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694453"/>
    <dbReference type="KEGG" id="loc:102694453"/>
    <dbReference type="CTD" id="497383"/>
    <dbReference type="eggNOG" id="KOG0383">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156124"/>
    <dbReference type="HOGENOM" id="CLU_020260_1_0_1"/>
    <dbReference type="InParanoid" id="W5MEH2"/>
    <dbReference type="OMA" id="ANPLYNF"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005618">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15523">
        <property type="entry name" value="PHD_PHF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102:SF6">
        <property type="entry name" value="PHD FINGER PROTEIN 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="499"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="639"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="573"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="695" mass="75007" checksum="62C5DEB34D9BBE56" modified="2014-04-16" version="1">MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKKVVEQLRKDLLVSKEQPEVKLQAQPALPPPPPGDGKHAPPAPAPTEAKALVQPAPAQLPLSLLAQNAPAQQKTVTVTPVIATKTLPLVLKAAAQTMPASVVTQRPTVAMVTAISNTQKTSANTDMQNAPINLQTSSKLASPGTEAVRIVSKNAVTLVQATTTAHTIKVPQFIPPPRLTPRPNFLPQVRPKPITPNNVPIAPAPPPMLAAPQLIQRPVMLTKVTATSLPTATGSIHQVRIMNGQPCATIAKTISPAQLTSIVITAPGTRIAGSQTLQISSANTDAKTVKVQGGTEDKSPVTVSPSPPPPAPAPPKPKREDNPQKLAFMVSLGLVTHDHLEEIQSRRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSPIHQGTRKRGRPPKFNSVPELGSLTPTSPPSSLPSSPAHEKTEIGGFHFPVLSQNLPLPSPNSGDGDIHEDFCTVCRRSGQLLMCDTCSRVYHLDCLDPPLKAIPKGMWICPKCQDQILKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLVKWSTELKQERDLLEQKVKQLSNSITKCMETKNTMLARQKEMQASLEKVKRLFRLVQGTQGPTKPAEGAQDAEGVANGTDGGGGGSREEDDTRSNGKTSEPSQQPAVTLSSSDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5MEX4</accession>
    <name>W5MEX4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H4]-lysine(20) N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.361</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60344"/>
            <dbReference type="Rhea" id="RHEA-COMP:15554"/>
            <dbReference type="Rhea" id="RHEA-COMP:15555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.361"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.361" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01005140">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MEX4"/>
    <dbReference type="FunCoup" id="W5MEX4">
        <property type="interactions" value="771"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000006933"/>
    <dbReference type="Ensembl" id="ENSLOCT00000006941.1">
        <property type="protein sequence ID" value="ENSLOCP00000006933.1"/>
        <property type="gene ID" value="ENSLOCG00000005740.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686646"/>
    <dbReference type="KEGG" id="loc:102686646"/>
    <dbReference type="CTD" id="751629"/>
    <dbReference type="eggNOG" id="KOG1085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163293"/>
    <dbReference type="HOGENOM" id="CLU_047978_0_1_1"/>
    <dbReference type="InParanoid" id="W5MEX4"/>
    <dbReference type="OMA" id="PEVACHT"/>
    <dbReference type="OrthoDB" id="5560686at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005740">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005700">
        <property type="term" value="C:polytene chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140944">
        <property type="term" value="F:histone H4K20 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043516">
        <property type="term" value="P:regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10528">
        <property type="entry name" value="SET_SETD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000021">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051760">
        <property type="entry name" value="KMT5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016858">
        <property type="entry name" value="KMT5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047266">
        <property type="entry name" value="KMT5A-like_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167:SF1">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027717">
        <property type="entry name" value="Histone_H4-K20_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51571">
        <property type="entry name" value="SAM_MT43_PR_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="211"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000006933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="347" mass="39272" checksum="F0A34F8C0B43A115" modified="2014-04-16" version="1">MARGKKKTPNHGKSKTEDIAHTTEQLKGKKENKPEMNGDIFYNGQSSIRPFLSPSKPRSPLHDENSIAHIHDGKSPGKRTVQQGSTKKEEEKQNGINPEVSITKSEESEAKRLESSGTESLPKYLELEAARLHSSADMTPENNGADRSSPKTETRRKVKVKSHRTADSKNSQNRKVTDYFPIRRSSRKSKTELKCEEKKQIDELIKSGIEEGMKVQHIEGKGRGVFATTCFRKGQFVVEYHGDLLQMTDAKKREAEYAQDPSTGCYMYYFQYLSKTYCVDATKETSRFGRLINHSKNGNCQTKLHDINGIPHLILVASRDIDEGEELLYDYGDRSKASIAAHPWLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MFI4</accession>
    <name>W5MFI4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-L-isoaspartate O-methyltransferase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PCMTD1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Substrate recognition component of an ECS (Elongin BC-CUL5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Specifically binds to the methyltransferase cofactor S-adenosylmethionine (AdoMet) via the N-terminal AdoMet binding motif, but does not display methyltransferase activity. May provide an alternate maintenance pathway for modified proteins by acting as a damage-specific E3 ubiquitin ligase adaptor protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01004452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01004453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006633995.1">
        <property type="nucleotide sequence ID" value="XM_006633932.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209167.1">
        <property type="nucleotide sequence ID" value="XM_015353681.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209169.1">
        <property type="nucleotide sequence ID" value="XM_015353683.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209170.1">
        <property type="nucleotide sequence ID" value="XM_015353684.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015209171.1">
        <property type="nucleotide sequence ID" value="XM_015353685.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047617.1">
        <property type="nucleotide sequence ID" value="XM_069191516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MFI4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007143"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007151.1">
        <property type="protein sequence ID" value="ENSLOCP00000007143.1"/>
        <property type="gene ID" value="ENSLOCG00000005913.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695014"/>
    <dbReference type="KEGG" id="loc:102695014"/>
    <dbReference type="CTD" id="115294"/>
    <dbReference type="eggNOG" id="KOG1661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183032"/>
    <dbReference type="HOGENOM" id="CLU_029295_0_0_1"/>
    <dbReference type="InParanoid" id="W5MFI4"/>
    <dbReference type="OMA" id="XLTQIMR"/>
    <dbReference type="OrthoDB" id="10257972at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000005913">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000015">
        <property type="entry name" value="Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF4">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="358" mass="41073" checksum="37393212D46F6B7F" modified="2014-04-16" version="1">MGGAVSAGEDNDDLIDNLKEAQYIRTEKVEQAFRAIDRGDYYLEGYRENAYKDLAWKHGNIHLSAPCIYSEVMEALKLQQGLSFLNLGSGTGYLSTMVGLIIGPFGVNHGVELHTDVVEYAKEKLDDFIKNSDSFDKFEFCEPSFVVGNCLEISSDSHQYDRIYCGAGVQKDHENYMKILLKVGGILVMPIEDQLTQITRTGQSTWESKNILAVSFAPLVQPNRTDGNKPETVKLPPVSVRNLQDLSRIYIRRTLRNLINEEMQAKGTPQKMPQKRKRRRCRRHRINTYVFVGNQLIPQPVESEEDERMEEDNKEEEEKDSNEGLKQEEPPQNILREKIMTLPLPESLKAYLTYYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MG22</accession>
    <name>W5MG22_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adhesion G protein-coupled receptor G3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01020942">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MG22"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000007331"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007339.1">
        <property type="protein sequence ID" value="ENSLOCP00000007331.1"/>
        <property type="gene ID" value="ENSLOCG00000006072.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="InParanoid" id="W5MG22"/>
    <dbReference type="OMA" id="CALGWMW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG23"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006072">
        <property type="expression patterns" value="Expressed in bone element and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000051">
        <property type="entry name" value="Adhesion G protein-coupled receptor G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000222">
        <property type="entry name" value="Adhesion G protein-coupled receptor G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF326">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="107"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42856" checksum="C2E18D1C713C887E" modified="2014-04-16" version="1">MYKANFTANILHNQAVGVSVSNTTVSGLKNPINITIFLEESLKANQIPKCVFLDFTATGRSNNHWKDYGCKTVKHEDYINCSCDHLTYFAVLLQVSASVSQYHLKILTIISKVGSGISLVFLIVTIILYIVGRRTTNDNSLKIHINLATALILLNVHFLPSEEVAALRNDSLCRYVAILLHYSLLCTFTWMAIEGFHLYILLCKVFSIYIRKYILKLCLVGWGFPAVVVILVVCADKDVYGLREIPTSEDNGTSIFMIMRCYINNITVNYVTSLCLFVLVFLFNLIMLSITVSKIMSMRSRKTPGENRKAFKDICGILGITCLLGTSWGLVFLSFGPVNLPSLYLFSILNSLQGFFIFLWYFSSKQRAGANTTSSDTHSTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGT1</accession>
    <name>W5MGT1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">General transcription factor IIE subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transcription initiation factor IIE subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with SND1. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01020378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01020379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGT1"/>
    <dbReference type="FunCoup" id="W5MGT1">
        <property type="interactions" value="1134"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007590"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007599.1">
        <property type="protein sequence ID" value="ENSLOCP00000007590.1"/>
        <property type="gene ID" value="ENSLOCG00000006285.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2593">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016696"/>
    <dbReference type="HOGENOM" id="CLU_051021_1_0_1"/>
    <dbReference type="InParanoid" id="W5MGT1"/>
    <dbReference type="OMA" id="DAIKWKV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG17"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006285">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000087">
        <property type="entry name" value="General transcription factor IIE subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021600">
        <property type="entry name" value="TFIIE_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11521">
        <property type="entry name" value="TFIIE-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="16">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="348"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="442" mass="50060" checksum="3E56D34A11EA0B13" modified="2014-04-16" version="1">SVMTEPEVLTEVPASLKRLAKQVVRGFYGVEHALAVDILIRNPCVKEEDMLELLKFDRKQLRSVLNTLKGDKFIKCRMRVETAPDGKTTRHNYYFINYRLLVNVVKYKLDHMRRRIETDERDSTNRASFKCPCCFSTFTDLEANQLFDPMTGTFRCTFCQTEVEEDESALPKKDARTLVARFNDQIEPIYILLRETEEINLSYDLLEPEPAEIPALKQSRERAAAAAAGAASGPHRENWATKGSAYEDLYTQKVIISMEEQEDQKRLAGEGKTPKERPIWLTESTVQGAYSELDDIKHSAEAMDGLREGEDGHHMQPDENEEVMRALLIHEKKGTGLAAGLAGAAAMAGVSQSGNHSDSESDTSESDEDSPPHPAPQSYLKNEEEDEDDFEEVGEEPTVLVAGRPFLYHEVSQRPELVEQMTPEEKEAYIEMSQHLFQAMYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MGW1</accession>
    <name>W5MGW1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transmembrane protein 132C-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM132 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01005166">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015221913.1">
        <property type="nucleotide sequence ID" value="XM_015366427.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MGW1"/>
    <dbReference type="FunCoup" id="W5MGW1">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007620"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007629.1">
        <property type="protein sequence ID" value="ENSLOCP00000007620.1"/>
        <property type="gene ID" value="ENSLOCG00000006307.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689698"/>
    <dbReference type="eggNOG" id="KOG4789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154702"/>
    <dbReference type="HOGENOM" id="CLU_009871_0_0_1"/>
    <dbReference type="InParanoid" id="W5MGW1"/>
    <dbReference type="OMA" id="VRGSCAM"/>
    <dbReference type="OrthoDB" id="10026202at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006307">
        <property type="expression patterns" value="Expressed in camera-type eye and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055422">
        <property type="entry name" value="Ig_TMEM132_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055423">
        <property type="entry name" value="Ig_TMEM132_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055421">
        <property type="entry name" value="TMEM132_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031436">
        <property type="entry name" value="TMEM132_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031435">
        <property type="entry name" value="TMEM132_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF23">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 132C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23481">
        <property type="entry name" value="Ig_TMEM132_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23486">
        <property type="entry name" value="Ig_TMEM132_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23039">
        <property type="entry name" value="TMEM132_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15706">
        <property type="entry name" value="TMEM132_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15705">
        <property type="entry name" value="TMEM132_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866071" evidence="8">
        <location>
            <begin position="26"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="922"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 N-terminal" evidence="9">
        <location>
            <begin position="49"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 second Ig-like" evidence="13">
        <location>
            <begin position="128"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 cohesin-like" evidence="12">
        <location>
            <begin position="282"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="11">
        <location>
            <begin position="429"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 fifth" evidence="14">
        <location>
            <begin position="529"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="15">
        <location>
            <begin position="666"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 C-terminal" evidence="10">
        <location>
            <begin position="891"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1096" mass="122034" checksum="2E68755BB5A6869A" modified="2014-04-16" version="1">MCFFTHLWRMLQIYTATILTIVTVSQEFESRELSDSMKSPSSFPVNLPVSYQITNANSFFFLKEISQDIMRNSSMQIHTEPFVILKTSQPPVISASYGPLSVEQTVPLHFMQTADLFRTSTKFTFDWKIQSYILTQKVYSSTPKMRILFYISGRDWEAQGTEGAMDDLPCVIVYAFWQTQEVRSSCSIKGDLGICVAELEPVLSWFSPASETTTRERPDQSEGNSVELYYTARPNANGGCSSEDVKKWGRTEQYESDDGSVTPMQRIGSVRLFQVPQSEAYLTQLKLGEAIVIQTSSKPLKKTDIATFSVFVSSGSAVDKFILRAVIKKNVSFLNARPSNSVLWEINLQKEEPENDGTITMLCQKKSDVSRKRPEDSLVEILQLDFEAEELSGHAESQGVTWQLEYPGSAKPVDEGVMPIYTTRRDFVGLAPLVMDTEILNTAVLTGKKVMVPVRVVAVEEDSSATDVSDFADCRSSDDDVLKVSDRCDYVFVNGKEMRGKVRITVNFFYGYLSAQLEMTVWIPRLPLQIEVSDTELSQIKGWRVPISTSKRPSWESDEEEEDRKGRGCMLQYQHALVRVFTHFVAEQSDPREQLAYFLGPDWQVDITELVRYFLKVEDPRIARLQAGRILAGRDVGVTTIQVLSPLSDSILAEKTVTVLDDKVAITELGVQLVTGLSLSLQLSPGSNRAIIATATTQELLHSPKQEAQISSWIQFSDGSMSPLDIYDSSYYMLSVTSLDEAVVSVRQDPSERFPVVLAEGEGQGAAVKVEMVISEVCQKSKRKSTLAVGSGSVRVSFHPSARGNKEDRSSDYGNDGEELENRKNARKQMTPSQGMTGTDGRFYGSSLSDREESAMRKITTTAKSVVRDVAMGSISSGAGWDHSNIIGYTNFPSQVDVPRNNGAEVESDLAQTPRSLTDLEIGMYALLGVFCLAILVFLLNCVSYVLKFRRKQAPVQGQDTVSHMHDWVWLGTDAELVMNMPGTPSQHEKHNTTVIDIGLGLENGANLVSGAVQQTDPMCGSMQSIRAKHKNESLNSPTTKRKRVKFTTFTSISLDNRLPSTDSALKDHEMDIKWVGQEENCVESKVVNGELLDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MHX7</accession>
    <name>W5MHX7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="4 14">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="function">
        <text evidence="14">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="4 14"/>
    <dbReference type="EMBL" id="AHAT01024300">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MHX7"/>
    <dbReference type="FunCoup" id="W5MHX7">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000007986"/>
    <dbReference type="Ensembl" id="ENSLOCT00000007996.1">
        <property type="protein sequence ID" value="ENSLOCP00000007986.1"/>
        <property type="gene ID" value="ENSLOCG00000006609.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="HOGENOM" id="CLU_054456_1_0_1"/>
    <dbReference type="InParanoid" id="W5MHX7"/>
    <dbReference type="OMA" id="CATNMAA"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG18"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006609">
        <property type="expression patterns" value="Expressed in camera-type eye and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF13">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="16">
        <location>
            <begin position="40"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000007986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="304" mass="32507" checksum="8504605C334912A2" modified="2014-04-16" version="1">MCLNSCSSCVPEGNSSMETSHDEYLQTAEWLLSHTAHRPKVGVICGSGLGALADALKSQDSFSYTDIPHFPQSTVQGHAGRLVFGELGGKACVCMQGRFHMYEGHSLGKVTFPVRVFKLLGVETLIVTNAAGSLANSYKTGDIMIIKDHINVPGLAGLNPLIGPNDEKFGPRFPPMSGAYDKALRRMALDICKSRGYSQFVQEGVYCMVGGPNFETIAEARLLHKLGADAVGMSTAPEVVSASHCGLKVLGLSLITNRVTKDYEDTESVDHGKVLGVSKMRSETLQGLVSELVAQLDINGTTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MIE9</accession>
    <name>W5MIE9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heparan-sulfate 6-O-sulfotransferase</fullName>
            <ecNumber evidence="10">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56604"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:140604"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the sulfotransferase 6 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="10"/>
    <dbReference type="EMBL" id="AHAT01015238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01015239">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637745.1">
        <property type="nucleotide sequence ID" value="XM_006637682.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MIE9"/>
    <dbReference type="FunCoup" id="W5MIE9">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008158"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008168.1">
        <property type="protein sequence ID" value="ENSLOCP00000008158.1"/>
        <property type="gene ID" value="ENSLOCG00000006754.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687855"/>
    <dbReference type="KEGG" id="loc:102687855"/>
    <dbReference type="CTD" id="553162"/>
    <dbReference type="eggNOG" id="KOG3955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183071"/>
    <dbReference type="HOGENOM" id="CLU_027877_1_0_1"/>
    <dbReference type="InParanoid" id="W5MIE9"/>
    <dbReference type="OMA" id="NHIINKW"/>
    <dbReference type="OrthoDB" id="406981at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000006754">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017095">
        <property type="term" value="F:heparan sulfate 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000852">
        <property type="entry name" value="Heparan-sulfate 6-O-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001933">
        <property type="entry name" value="Heparan-sulfate 6-O-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF1">
        <property type="entry name" value="HEPARAN-SULFATE 6-O-SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="408" mass="48582" checksum="423EBCDA9471D344" modified="2014-04-16" version="1">MNRIGRNMVERTSKFLLIVVGSIFFMLILYQYVAPGMMNFGSPHGYLVEDDTDIFPTPDPHYVKKYYFPIRDLDRTVDFQIKGEDVIVFLHIQKTGGTTFGRHLVQNVRLEVPCDCRPGQKKCTCYRPNRKETWLFSRFSTGWSCGLHADWTELTNCVPGVLNKKENKFKNLRKFYYITLLRDPVSRYLSEWRHVQRGATWKTSLHMCDGRTPTPEELPSCYEGTDWSGCTLQQFMDCPYNLANNRQVRMLADLSLVGCYNMSFIPEKKRAQILLESAKKNLRDMAFFGLTEFQRKTQYLFERTFNLKFIRPFMQYNSTRAAGVDVDNDTILRIEELNDLDMQLYDYAKDLFQQRYQYKRQLDRREQRIKNHEEYRQHRSNEEPPREEPEEPARLPTEDYMNHIINKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5MJL6</accession>
    <name>W5MJL6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Interleukin 12 receptor subunit beta 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015973">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MJL6"/>
    <dbReference type="FunCoup" id="W5MJL6">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008575"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008585.1">
        <property type="protein sequence ID" value="ENSLOCP00000008575.1"/>
        <property type="gene ID" value="ENSLOCG00000007083.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRRE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159829"/>
    <dbReference type="InParanoid" id="W5MJL6"/>
    <dbReference type="OMA" id="KWAKECT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007083">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019955">
        <property type="term" value="F:cytokine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF2">
        <property type="entry name" value="INTERLEUKIN-12 RECEPTOR SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867860" evidence="13">
        <location>
            <begin position="24"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="122"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="217"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="412"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="509"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="711"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000008575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="838" mass="94437" checksum="C94CCC57D02C12CE" modified="2014-04-16" version="1">RSLMPVIWSWFTLNLIFLLYSRGASDLNKLCAIFATNGTLGKLGSSIEVYCTFKKFVTGRCRKAIFRDTNQEYNFIEYNSTTLLLKTELRKHRTVFVCKCAPDEPLYEPCGLDILAGLSPDVPQNLICIQNGNYGDITCRWKKGSNTHIETTYELWVKTESYNGTKHLASHSGSEYVIFPVLHSEVKYSVWVTASNKLGTASSPSLKFTLNDIVKFHPPHVTVVNCSSQNCTLHWRDIEKAQLLEVRYQIVGESSWIYQTFKATANNTLSCGGLQPFTTYTFQARCKLSCTEGIWSEWNDAVNTTTGEADPLQKLDVWHIVESSESKNKFVSVVWKKLTRYEARGVVLGYEVLVEDQVEKKLSEVYITNSSYSKPVFCSHCTVTVSALNSKGSSPPATISIPILSDLSSFPSPRNVTCLPHSNHSIMISWEPPLDSPVPLHSYIVEWSQAYKDTKLSLTWKRLSHHQLSTVISENVQPCVCYKGAVYALYDQGLGKAGFVNAYSWQSVPSQGPKLNVRQWDNKSLTVYWKEVSEQDKRGCLKSYNLYLENIQGHLQKYGPIDPTHRSYTISDLQPAASYYLWMTASTDTGEGRKGEKYTFTTYPEREDDQLLVIWPTTVLVSFLVWLFLVFLCRVSVQRRPSPCCFCIIPGNVPDPANSKWAKEYAAVKGHMKLVYELYNNSTSSNEEPNTIEIEEIPEDSLNVSRTVSERNILPSGEPKEQQSQERDSGQTSCPPCQSQGLGTYKGQFTCPYIKSLSQESEFSTKTQISCSTDTTGDYIPSNVLLSGTSEEDSNDFMEDELMEFSSCHNSPFLEPMITFGGKLTLDAVKIDCSSFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5MK89</accession>
    <name>W5MK89_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 18">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homo- and heterotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="EMBL" id="AHAT01032415">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MK89"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000008798"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008809.1">
        <property type="protein sequence ID" value="ENSLOCP00000008798.1"/>
        <property type="gene ID" value="ENSLOCG00000007256.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155440"/>
    <dbReference type="HOGENOM" id="CLU_011053_0_0_1"/>
    <dbReference type="InParanoid" id="W5MK89"/>
    <dbReference type="OMA" id="KKTNLCH"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007256">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:membrane"/>
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    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:fructose-6-phosphate binding"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
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        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
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        <property type="entry name" value="Eukaryotic_PFK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Phosphofructokinase_I_E"/>
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        <property type="entry name" value="6-Pfructokinase_euk"/>
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        <property type="entry name" value="ATP_PFK"/>
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        <property type="entry name" value="PFK_vert-type"/>
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        <property type="entry name" value="Phosphofructokinase_CS"/>
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        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF59">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, MUSCLE TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PFK"/>
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    </dbReference>
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        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="20"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="403"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="403"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
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        <location>
            <position position="203"/>
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        </ligand>
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        <location>
            <begin position="210"/>
            <end position="212"/>
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        <location>
            <position position="266"/>
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        <location>
            <begin position="300"/>
            <end position="303"/>
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        </ligand>
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        <location>
            <position position="472"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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        </ligand>
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            <begin position="529"/>
            <end position="533"/>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <position position="567"/>
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            <begin position="574"/>
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            <position position="630"/>
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            <position position="656"/>
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            <begin position="662"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="788" mass="85599" checksum="0E45B493178EB9A3" modified="2014-04-16" version="1">SAMTSPSAVDPTRMGEGKAIAVLTSGGDAQGMNAAVRAAVRVGIYCGAKVFFVHEGYQGLVDGGHNIRPATWESVSMMLQLGGTVIGSARCQDFRAREGRTKAACNLVKLGITNLCVIGGDGSLTGANQFRSEWSELLADLVKTGKITAEEARRSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRTFVLEVMGRHCGYLALVTALACGADWVFIPEMPPEDNWEDHLCRRLSETRGRGSRLNVIIVAEGAIDKNGKPITSECVKNLVVKRLGYDTRVTILGHVQRGGTPSAFDRILGSRMGVESVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRAMAEGRFEDAIKLRGRSFENNWNTYKLLAHIRPPEGKSNFNIAIMNVGAPSAGMNAAVRSAVRIGIIQGHHMLAVHDGFEGLATGQIEPIGWGGVGGWTGKGGSNLGTKRVLPGKTLEEISLNIAKFNIHALVIIGGFEAFSGGLELVSGREKYEELCIPLVIIPATVSNNVPGSDFSIGADTALNTITNTCDRIKQSAAGTKRRVFLVETMGGYCGYLATMAGLAAGADAAYIFEDPFTIHDLETNVEHLVQKMKTTVKRGLILRNEKCNSNYTTDFIFNLYSEEGKGTFDCRKNVLGHMQQGGTPTPFDRNFGTKLGARSVLWLTDKLKECYRHGRVFANTPDSACVLGMRKRALLFQPLAELKEQTDFEHRIPKEQWWLKLRPILKILAKYQITLDTSEKAHLEHVIRKRPGEPLQLSGLQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5MKA0</accession>
    <name>W5MKA0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with tetraspanin-3/TSPAN3. Interacts with OCLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell junction</location>
            <location evidence="11">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01015260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006637751.1">
        <property type="nucleotide sequence ID" value="XM_006637688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MKA0"/>
    <dbReference type="FunCoup" id="W5MKA0">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000008809"/>
    <dbReference type="Ensembl" id="ENSLOCT00000008820.1">
        <property type="protein sequence ID" value="ENSLOCP00000008809.1"/>
        <property type="gene ID" value="ENSLOCG00000007277.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102689077"/>
    <dbReference type="KEGG" id="loc:102689077"/>
    <dbReference type="CTD" id="436897"/>
    <dbReference type="eggNOG" id="ENOG502QSDJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139496"/>
    <dbReference type="HOGENOM" id="CLU_094997_1_0_1"/>
    <dbReference type="InParanoid" id="W5MKA0"/>
    <dbReference type="OMA" id="SYQDNNH"/>
    <dbReference type="OrthoDB" id="9411914at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000007277">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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                <consortium name="Computational R&amp;D Group"/>
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                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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    <sequence length="222" mass="24870" checksum="BEE3583327373937" modified="2014-04-16" version="1">WERRFKRKHRRRSDDGASSPKRRRLAEEAVRLFYQAPWQQGGGAWAPQLAAPPLQTAQQAAAPGQGVGSPPRAAPEPEAFSMEVEARVAAVQRLQEIEDRITLEDDDEDDLETESADRKPVLVISDSLREGLQRGIADIIPKKVVESMNHLCMELVLWRPPPEVLPRRLKDSMQRVQQLAASSSKEPLLVKNTDTEAQQHRSCSMLYTGPGSQAGMEEDVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for the splicing of pre-mRNA.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="8">Belongs to the splicing factor SR family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSLOCG00000008050.1"/>
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        <property type="term" value="C:nuclear speck"/>
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        <property type="term" value="C:U2-type prespliceosome"/>
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        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
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        <property type="term" value="P:spliceosomal complex assembly"/>
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        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0507">mRNA processing</keyword>
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    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="143"/>
            <end position="225"/>
        </location>
    </feature>
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            <begin position="1"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="349" mass="39139" checksum="191E7A01961B2549" modified="2014-04-16" version="1">MPTLSESLRFPSMPAQVTEPFLSPPERDKENRHRRRSPTRSRSRERKRRSRERRSRDRRSDSKDRRQRRSSRSPHREKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNVSDQAIAGLNGMQLGDKKLLVQRASVGAKNATLTSINQSRVSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MN84</accession>
    <name>W5MN84_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:172182</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01020077">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MN84"/>
    <dbReference type="FunCoup" id="W5MN84">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000009854.1">
        <property type="protein sequence ID" value="ENSLOCP00000009843.1"/>
        <property type="gene ID" value="ENSLOCG00000008105.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QU10">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165617"/>
    <dbReference type="HOGENOM" id="CLU_066884_0_0_1"/>
    <dbReference type="InParanoid" id="W5MN84"/>
    <dbReference type="OMA" id="AMLCSRI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008105">
        <property type="expression patterns" value="Expressed in testis and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043450">
        <property type="entry name" value="CCDC89-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34768:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34768">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="71"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="355" mass="41812" checksum="48172591986D3791" modified="2014-04-16" version="1">MASPHRTPRDLKKMITDTKQEMDDVHTALEKLRGLSQDDKTESALLRSRIDEQSSLICILKQRADEMLLRCQALERINSELETLREDVHKELENERKRSSQLEQRFLDLAANHQELINFKDEYKRQNAELKEENERLQQENENLFCKELQEKEEVILQLTQELKDLAEQHKILEAEYKEKTNGFQTKLKELIITHQTKEASLQGELQDTQKQLKDAVEMCTVELDLQLRRAMEKDALEESQLQQKLEALVKEKDELLDLSMQRGRIIQDKQKEIQELEEKREEAEKARATAEERFEKEAAAVEANLKVQDLQRCLDESEQSYNDLKKDFEAYKKHSSDLLAKEKELNAKLRHMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5MNK5</accession>
    <name>W5MNK5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Palmdelphin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the paralemmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007602">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01007603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MNK5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000009964"/>
    <dbReference type="Ensembl" id="ENSLOCT00000009976.1">
        <property type="protein sequence ID" value="ENSLOCP00000009964.1"/>
        <property type="gene ID" value="ENSLOCG00000008208.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S32R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204330"/>
    <dbReference type="HOGENOM" id="CLU_636092_0_0_1"/>
    <dbReference type="InParanoid" id="W5MNK5"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008208">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46881">
        <property type="entry name" value="PALMDELPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46881:SF1">
        <property type="entry name" value="PALMDELPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000009964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="421" mass="45816" checksum="31EE5836FD611BB4" modified="2014-04-16" version="1">TMDEAEKYQQRLQAIAEKRRIQEEQERARREMEEEKLRLQQMKRKSLRDQWLMEGPPLSPEVPSPRSPLWGLQAQQIEERIDKLQTESKRLAEEAANLDRAEGTPPQNDAGEAEREEGSKEGSQSLHSSQNPQKVDVEGEPKTNGSVSATEVSGDENPEPSPATRAQENAPAERPGGQVPQGTAEITDHTGQGAITLTFLGFTEASPEEDFEEAAGTVIRAERVIITDEGDELSMDSEEERLKVSNSKEQPQERPPTEEKVQTDSKVEKMQVPSSEVDLPVESKELPVEIQTAASQHPPITTPSSEAEPPHPPKSDMEPEKEGLDEGKTTDEKPALENHIDAAAQAAEPAEAQQAEQFQDIPLGGQAPATPAEIEPLVAASTAAHNSATPSAAVARAEDGKAEGPEAPQPKHKSCQCCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MPJ0</accession>
    <name>W5MPJ0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01007580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MPJ0"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010312.1">
        <property type="protein sequence ID" value="ENSLOCP00000010299.1"/>
        <property type="gene ID" value="ENSLOCG00000008466.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154641"/>
    <dbReference type="HOGENOM" id="CLU_013137_8_1_1"/>
    <dbReference type="InParanoid" id="W5MPJ0"/>
    <dbReference type="OMA" id="WIDDREK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008466">
        <property type="expression patterns" value="Expressed in ovary and 4 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF155">
        <property type="entry name" value="CD276 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868527" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="233" mass="25979" checksum="48A45A84C74D808D" modified="2014-04-16" version="1">LMVICLFNSAILLSGTPRVHVLTPAITALCGSDVRIDCQFHTSGMRKCFDVEWLQKRQGRTNSIHLFHQSPELSRFQDHSVTGRSTMFPSAVTNGNASLLISNVEMTDEGEYICFIVMDGVGYRDTAVLTVVGRYSSTVFQLKSVASCKQVLEQEAVSLSCVSRKGFPAALVSWIDDREKSYSQEAITELTLTPEGLYDVHSRLKRVFTSQRRVCCIITNSPLQESKRSCVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5MQV9</accession>
    <name>W5MQV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidic acid phosphatase type 2D</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01007523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006631723.1">
        <property type="nucleotide sequence ID" value="XM_006631660.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MQV9"/>
    <dbReference type="FunCoup" id="W5MQV9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000010768"/>
    <dbReference type="Ensembl" id="ENSLOCT00000010783.1">
        <property type="protein sequence ID" value="ENSLOCP00000010768.1"/>
        <property type="gene ID" value="ENSLOCG00000008851.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683657"/>
    <dbReference type="KEGG" id="loc:102683657"/>
    <dbReference type="CTD" id="559124"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164486"/>
    <dbReference type="HOGENOM" id="CLU_021458_3_0_1"/>
    <dbReference type="InParanoid" id="W5MQV9"/>
    <dbReference type="OMA" id="WHETENN"/>
    <dbReference type="OrthoDB" id="8907274at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000008851">
        <property type="expression patterns" value="Expressed in muscle tissue and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000030">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF94">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000010768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="37038" checksum="FE9A941B1E1428B3" modified="2014-04-16" version="1">MQNYKYGPTASGVLPRDAELHARLGDLGESGSPRENGSGRSHTDCSSHAQQKRRKMLVCLDVLCLFVASIPFLACELKTVEPYKRGFFCKDESIAYPYLDTESIPDGLLISGGIIITGLSIALGECYRVRYLNVKSRAFITNLYVSTLYKEIGSFLFGCCVGQSLTNMAKLSVGRLRPNFLSVCGLNYSNLNCTEGSYIVNFECPSIKNNEEYAKLVTEARKSFYSGHASFAMYTMLYLAFYLQARFTWHGARLLRPLMQFLLVLLAVYTGLTRVSDYKHHPSDVLTGFLQGALVAYWVAFYISTMFKPPSQETTENVNSLDSPVSSQQTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRR3</accession>
    <name>W5MRR3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING finger protein 150</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01015445">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRR3"/>
    <dbReference type="FunCoup" id="W5MRR3">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000011072"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011088.1">
        <property type="protein sequence ID" value="ENSLOCP00000011072.1"/>
        <property type="gene ID" value="ENSLOCG00000009076.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156171"/>
    <dbReference type="InParanoid" id="W5MRR3"/>
    <dbReference type="OMA" id="MCKINIL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009076">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000016">
        <property type="entry name" value="Ring finger protein 150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF27">
        <property type="entry name" value="RING FINGER PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004868649" description="RING finger protein 150" evidence="13">
        <location>
            <begin position="35"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="268"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="434" mass="48234" checksum="2980D13478A9AB9B" modified="2014-04-16" version="1">MAMSLIQACRSLALSTWLLSFCFVHLLCLDFTVAEKEEWYTAFVNITYLDPATSEIKTEKTECGRYGEHSLKQDAKGVVVMPSSLHDRQACDPNTKFTVPPNANTWIALITRGNCTFKEKIRHAANQNASAVVIFNVGSGNANETITMPHPGTGDIVAIMIPEPKGREIMSLLEKNVTVMMYITIGTRNLQKYVSRTSVVFVSISFIVLMIISLAWLVFYYIQRFRYANARDRNQRRLGDAAKKAISKLQVRTIKKGDKETESDFDNCAVCIEGYKPNDVVRILPCRRHLFHKNCVDPWLQDHRTCPMCKMNILKALGIPPNADCSDEILPDYEVSIGGPPTNQVTGASDVTVNESSVVLDPAVRTVGLPQVFRDSELLPPAGESNIATRSEQEPTVSSDSDTSLIISVEVGLSDVELSTDQDCEETKTRKNAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5MRW4</accession>
    <name>W5MRW4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="11">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="11"/>
    <dbReference type="EMBL" id="AHAT01002893">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MRW4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000011123"/>
    <dbReference type="Ensembl" id="ENSLOCT00000011139.1">
        <property type="protein sequence ID" value="ENSLOCP00000011123.1"/>
        <property type="gene ID" value="ENSLOCG00000009118.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009048"/>
    <dbReference type="HOGENOM" id="CLU_000914_0_0_1"/>
    <dbReference type="InParanoid" id="W5MRW4"/>
    <dbReference type="OMA" id="IQERWCC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009118">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000001">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000013">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF10">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="13">
        <location>
            <begin position="61"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="915"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="975"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1207"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="932"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="975"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1224"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1258"/>
            <end position="1274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000011123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1274" mass="142991" checksum="D6800381B5FF8EF6" modified="2014-04-16" version="1">MSVGEAEADHPRFFVGGEDGEGDELLDSGRVMGYDCLYENVEEEEEGDEDEEYDSPPERQIVVGICAMSKKSKSKPMKEILERLCLFKYITVVIFEEDVILNEPVENWPLCDCLISFHSKGFPLDKAVAYEKLRKPFVINDLDMQYHIQDRREVYRILKAEGILLPRYAVLNRDPAKPEECSLVEGEDHVEVNGEIFQKPFVEKPVCAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFQIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQRFFDLFEKCDGYKTGKLKLKKPKQLQEVLDIARQLLAELGQNNDSEIEESKAKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPKTSSEEEDNRRDDPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQRDRDFTAEDYEKLAPTSSSSLIKSMQMIKNPVKMCDKVYSLIQSLTSQIRQRMEDPKSADIQLYHSETLELMLRRWAKLEKDFKMKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMEIYRLSKALADIVIPQEYGIAQAEKLDIAKGYCTPLIRKIRSDLQRTQDDDTVNKLHPVYSRGVMSPERHVRTRLYFTSESHVHSLLSILRYGALCDETKDDQWKRAMDYLNVVSELNYMTQIVIMLYEDPNKDPSSEDRFHVELHFSPGAKGCEEDKNLPSGFGYRPASRENEGPRKKSINDSDEESSAAKRDEPDRALFRPMVSDPIYIHRKSPLPRSRKIGSVEEESPLSVSSPESIGTWIHYTCGVGTGRRRRRSGDQITSSPVSPKSLAFTSTIFGSWQQVLSENNSHTRVGRLHSEQKHSGMVLLGSHCAGLFSTMVLGGSSSAPNLQDYARAHRKKLTSSGFLDDATRGSAVKRFSISFARHPTNGFELYSMVPSICPLETLHNSLSLKQVDDFLASVAASSNETFLETPTSSPASTLGYGTVPSRKTSLNTYTPARILPSPFSHSSSKKASDRASVVSSGPSSPVSSAGPPSPADLSPHNIPGGNVLMQQTSDEQETPKGSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5MSX7</accession>
    <name>W5MSX7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PTTG1 interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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    <sequence length="170" mass="19229" checksum="2E667070F4042F5B" modified="2014-04-16" version="1">MMELRILSPLVVVVLVNLAVVYAQTSAPGVSCATYSNTSCEQCLKNVSCLWCKTNTRCIDYPVRTILPPHSLCPLSSARWGMCWMDFQALIIAISVIAGIILIAIFICCFCCCKCEKIGIPKLDAKFAHQGEKRKARQEERKAEMKMRHDEIRRKYGLTKSNPYSKFENN</sequence>
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    <name>W5MU36_LEPOC</name>
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        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
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        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
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                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="5">Belongs to the DOCK family.</text>
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        <property type="entry name" value="DOCK"/>
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        <property type="entry name" value="DOCK_C_lobe_A"/>
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        <property type="entry name" value="DOCK_C_lobe_C"/>
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        <property type="entry name" value="DOCK_N_sub1"/>
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        <property type="entry name" value="DOCKER_dom"/>
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        <property type="entry name" value="DOCKER_Lobe_B"/>
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        <property type="entry name" value="DOCKER_Lobe_C"/>
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        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
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    </dbReference>
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        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 2"/>
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        <property type="entry name" value="C2_DOCK"/>
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    </dbReference>
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        <property type="entry name" value="DOCKER"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="7">
        <location>
            <begin position="249"/>
            <end position="413"/>
        </location>
    </feature>
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        <location>
            <begin position="965"/>
            <end position="1376"/>
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    </feature>
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        <location>
            <begin position="1371"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1404"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <begin position="1371"/>
            <end position="1384"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1451"/>
            <end position="1460"/>
        </location>
    </feature>
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        <location>
            <begin position="1466"/>
            <end position="1498"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1499"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="1515" mass="174831" checksum="2E0E51E5A1818E6F" modified="2014-04-16" version="1">QGVFPASYIHLKEVIIEKRGGQEVVTSAEMPLVQEVTTTLREWGSIWKQLYVANKKEKVEQVRRLLWELVEWRSQLLSGTLPKDEFKELKQKVTSKIDYGNKILDLDLVVRDEDGNILDPDRTSVISLFRAHEEATAKINERIKEEMSNIQADHSGISARIQSSPTHSLYVFVRNFVCRIGEDSELFMSLYDPQKQAIIRSAXGLWVTMKALVGDIVQIRKEYPHLVDRTTVVARKLGFPEIIMPGDVRNDIYITLQGGDFDKYNKTTQKNVEVIMWVCDEEGKVVPNAICLGAGDRPVNEYRSVIYYQVKQPRWMETFKVAIPLEEMQRIHLRFMFRHRSSQESLFYSPSSLFLSWSLCSLPLSVTHPNSHPESAHQLSLSPGEVQSVSVSVLTCLSSVGLLGLLKWRTRPELLKENLEKLKIIDGEEVVKFLQDTLDALFNIMMEHSQTDDYDILVFDALIYIIGLIADRKFQHFNTVLEAYIKQHFSATLAYKKLMSVLKTYLDTSSRGEQCEPILRTLKALEYVFKFIVRSRMLYSQLYEGREQTEFEESLRRLFESINNLMKSEYTTTLLQQVSAVRCVSLFLFVSQQVVSQTSFQGDYNNQLLFEFYTCIPPDKLQRQKVQSMTEIVGSRVFKKQGNVSIRTLSPDPPEEGKGWSAEEGEGQSVSVALLPEALTLLASLLGAENVGPTFHHIQEIVLQLLRIVNRKVITLGREHALISRVVACMTAILSQMDDRHYSCYIETFSSMTDLVDFLMETFMVFKDLIGKHVYPSDWVGMIMVQNRVFLRAINTFADTMNRKFLDNNNFEVQLWNNYFHLAVAFITQESLQLEHFSPTKRSKILSKFSDMRRLIGFAIRDMWYKLGESVGVSVWPSPPILCSPEEMCQRVSKPMPLSFALCVCLCQLLFLSFAPCSLALAYTVCGYVFNIAGDCVSSGALKPRISRYVEIKMCSDFRRSGGTENQVEREGEPARYLYKLRDLHLDCENYTEAAYTLLLHTRLLKWSEEQCSPQLMVSDYHSSQTQRQLKESLYATIIDYFDKGKMWEEAISLCKELAEQYELEVFDYELLSQSLRQQAKFYENIMHILRPKPDYFAVGYYGQGYPTFLRNKVFIHRGKEYERREDFQSQLMSQFPSARRLNTTTLPGDDIKNSPLQYIQCFTVQPVLEIPPRLKNKPVPDQIINFYKSSYVQRFHYSRPVRKGSVDPNNEFASMWIERTTFFTSYKLPGILRWFDVKEMTHTTISPLENAIETMETTNEKILTMINQYQGDDSLPINPLSMLLNGIVDPAVMGGFAKYEKAFFTDEYVQQHPEDRDKLSRLKDLIAWQTPLLRAGIRLHGKRVTDDLRPFHDRMEDCFKQLKKKVEKEYGARELPEMDDRRSSRPRSMLRSYRQSLISISSLGSECGTPTKLHPESFGVPESPSSRAPPRPEDGGMVAEEPVEGEVRLRKSKKKRKRSSMIFISEERERQSTLDTRRLSKKQEFRSDTNLSEHSETSRGLYSTNSRSLPTITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUG0</accession>
    <name>W5MUG0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01012870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUG0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012019"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012040.1">
        <property type="protein sequence ID" value="ENSLOCP00000012019.1"/>
        <property type="gene ID" value="ENSLOCG00000009834.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVK4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008061"/>
    <dbReference type="HOGENOM" id="CLU_025135_0_0_1"/>
    <dbReference type="InParanoid" id="W5MUG0"/>
    <dbReference type="OMA" id="AHIFDCK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33488:SF2">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33488">
        <property type="entry name" value="ZGC:162509"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="642" mass="72646" checksum="18FD398C527B4ADA" modified="2014-04-16" version="1">RTMALSKTIKTITRAQDVRNDAKMIMKPYANWEEFLMPVPISIAILGEPVFISSKTDFSINKNPPKGFKPVSYAGWGAFNEAHKNMDQIHLLSGNVPEYVKMAVRLILQDDDKLIQLMITDQLQNISSIAEECKTRPELTEKKFILIQEQMEACVNAKAACGEEAERVRRKIEEFNLRKMATEEGKQQAKEVYDNMGRQLKEAQQEFKNAMYSLPNGWNVIGMNFVQGLTNAINMRLGLGSVPLILDFFKSHQEEKINLTNPIALGNVFAVSEILLSHVDQIAAFCNSDEIDWGSLYDQKKEYAVSEWVVKRVQGFINKQEECRPKSLALEALESCSDLTCIAPLGKCDEDQKQKLINDIKNLRRLALKFDSQSKACTGASPFTVTPPQLAKHQEENDTVKSAEKIAVENVRFRIEQNCAQLDQAQNMFQKILDNLGNYHKDLSEILTTDSSFEMKEIDLKTMIKMLMKGLDAMGKVKEQWEMVHLFQMISNIIKICLSTCLMDLIKTAGKASSSELNYSSRLFLKDMLYNYAFQASNIASLVNVISGSYTEISNTYLMDSISSLGKLMVLDRSNPDFDSERTNLQKGCEEAGKGIKILVLKSKDDFDKIAKSRMEKIESAFKTVLPPTSEEDIQVIRKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5MUU9</accession>
    <name>W5MUU9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MUU9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012158"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012180.1">
        <property type="protein sequence ID" value="ENSLOCP00000012158.1"/>
        <property type="gene ID" value="ENSLOCG00000009945.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157983"/>
    <dbReference type="HOGENOM" id="CLU_079452_4_2_1"/>
    <dbReference type="InParanoid" id="W5MUU9"/>
    <dbReference type="OMA" id="QHLCRII"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000009945">
        <property type="expression patterns" value="Expressed in zone of skin and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046426">
        <property type="term" value="P:negative regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03717">
        <property type="entry name" value="SOCS_SOCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF7">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="160"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="207" mass="23234" checksum="A895B94B5700F9B9" modified="2014-04-16" version="1">RTVYHNRTIESLINQDSVSEQQRGETTEELTRVENAMSHLHESGWYWGSLTAAQAKQVLNDALEGTFLLRDSSNPGYLLTLSVKTSLGPTHLRIEHSGGAFGFDSLVLTRPRLRQFSGVVDLVQHYALSCQQGTRSGSEPKSETKSSTHPSPPETTLQLILTRPLYKAPPSLQHLCRIVINQRSPNGTDLPLPRRLKDYLQEYPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5MVJ1</accession>
    <name>W5MVJ1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069046325.1">
        <property type="nucleotide sequence ID" value="XM_069190224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MVJ1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000012400"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012421.1">
        <property type="protein sequence ID" value="ENSLOCP00000012400.1"/>
        <property type="gene ID" value="ENSLOCG00000010130.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102696634"/>
    <dbReference type="eggNOG" id="KOG1354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
    <dbReference type="HOGENOM" id="CLU_021713_3_3_1"/>
    <dbReference type="InParanoid" id="W5MVJ1"/>
    <dbReference type="OMA" id="LVPMELI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010130">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="450" mass="51945" checksum="9E137414A9DBAA34" modified="2014-04-16" version="1">MSGVETRWTLVRVNGSKKKKVYEGTKADIISTVEFNQTGELLATGDKGGRVVIFQREPESKNEPYCQGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAHFLLSTNDKTIKLWKVSERDKRPEGYNLKDEEGRVKDLSTVTSLQVPVLKPMDLMVEVSPRRIFANAHTYHVNSISVNSDCETYMSADDLRINLWHLAITDRSFNIVDIKPANMEELTEVITAAEFHPYHCNLFVYSSSKGTLRLCDMRASALCDKHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYLLTRDYLTVKVWDLNMENKPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGTDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCTGGKRRKDDISVDSLDFTKKILHTAWHPAENIIAIAATNNLYIFQDKVNSEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MWB9</accession>
    <name>W5MWB9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DBF4 homolog A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01004669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MWB9"/>
    <dbReference type="FunCoup" id="W5MWB9">
        <property type="interactions" value="520"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000012678"/>
    <dbReference type="Ensembl" id="ENSLOCT00000012702.1">
        <property type="protein sequence ID" value="ENSLOCP00000012678.1"/>
        <property type="gene ID" value="ENSLOCG00000010346.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063909"/>
    <dbReference type="HOGENOM" id="CLU_446143_0_0_1"/>
    <dbReference type="InParanoid" id="W5MWB9"/>
    <dbReference type="OMA" id="CCPFESE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010346">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF22">
        <property type="entry name" value="PROTEIN DBF4 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="12">
        <location>
            <begin position="306"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="578"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000012678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="622" mass="69525" checksum="A2AE0419141775F5" modified="2014-04-16" version="1">MKNRVTSQDVNVKLQDSKSIDKTEQNKVTLKSVLQTANGSSGLKTKPLAGKLFYLDLPFSKTTEALEKDIQKLGGTIERFFSKDIRYLVSNKKEAKFVKSVGRNSPLSSPGSPNTTGNSSPCLSSRRGSCKVSSQDAADVSLAIKSRGKSLVEKVVKEQELIQTNRILSNALDWGVKILYIDDILSYIEKKKKDLPTDQPNLHTSKVSNATNAKTSVKKNEVGNLKKPFLKVEDQSRHYKPIYLSLPNLPELNFCLPAPCCPFESEKTNKDRDVKKQSNKVQRHIADEANCTKQLKAKLRALDLKEKKKRGYCECCVVKYENLKAHLSSERHKAFSTSDEYFVVDKVTSELVCDFVEIQNEKGERCSSATYSHASASAKTLDQRRKMHIDVDKCSNIEEASILWNHTAEKLLVSEKSTLQRKQNGSPSEKEGKNKTTVCPDLRLTSGFQNERKMLINLSTPCAAKISEASISVCTIELNLGNGVGQKTGKRTIGESTLGQSQTEDNLLGIPAKSQKIASSQLDVPESVESELFCNSNLNNLIIPDKGADNTDHYLSRTHSSPVGKLQRKVKPFRRTRRASERETLQKHSKQNDVELSPPLHNLWQLFQSSENVDWEFLGFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5MX97</accession>
    <name>W5MX97_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial glutamate carrier 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutamate/H(+) symporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 22</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial glutamate/H(+) symporter. Responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton. Plays a role in the control of glucose-stimulated insulin secretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-glutamate(in) + H(+)(in) = L-glutamate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01014338">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MX97"/>
    <dbReference type="FunCoup" id="W5MX97">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000013034.1">
        <property type="protein sequence ID" value="ENSLOCP00000013006.1"/>
        <property type="gene ID" value="ENSLOCG00000010612.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164224"/>
    <dbReference type="HOGENOM" id="CLU_015166_3_4_1"/>
    <dbReference type="InParanoid" id="W5MX97"/>
    <dbReference type="OMA" id="NEAVFWC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000010612">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015810">
        <property type="term" value="P:aspartate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000017">
        <property type="entry name" value="Solute carrier family 25 member 22a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF13">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER: GLUTAMATE), MEMBER 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="102"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="230"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="332" mass="35777" checksum="3E714D2EB650574D" modified="2014-04-16" version="1">KMSQQQISLPAKLINGGIAGIVGVTCVFPIDLAKTRLQNQRSGQQVYKNMMDCLVKTVRSEGYFGMYRGAAVNLTLVTPEKAIKLAANDFFRHLLSKDGKGLTVFKEMLAGCGAGTCQVIITTPMEMLKIQLQDAGRLAAQQRKKTVVPSTKLGATNTLLSRSYNVGPAPLVRKVSATQIAQELLRTQGIQGLYKGLGATLMRDVPFSVVYFPLFAHINRLGQPSPEENAPFYHSFLAGCIAGSTAAVAVNPCDVVKTRLQSLNKGANEESYNGVIDCVSKILRKEGPSAFLKGAGCRALVIAPLFGIAQVMYFVGVGEFILGYTPFNLYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5MYY8</accession>
    <name>W5MYY8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily B member 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Endoplasmic reticulum DNA J domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with HSPA5/BiP; interaction is direct. Interacts with ERN1/IRE1 (via the luminal region). Interacts with DERL1.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MYY8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000013597"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013626.1">
        <property type="protein sequence ID" value="ENSLOCP00000013597.1"/>
        <property type="gene ID" value="ENSLOCG00000011076.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166994"/>
    <dbReference type="HOGENOM" id="CLU_077239_0_0_1"/>
    <dbReference type="InParanoid" id="W5MYY8"/>
    <dbReference type="OMA" id="DEPHIHW"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011076">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004866910" description="DnaJ homolog subfamily B member 9" evidence="6">
        <location>
            <begin position="26"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="171" mass="20326" checksum="37261B12B4FADD8B" modified="2014-04-16" version="1">MKEYFPTVYLAIVLCQVLSVDLTDAKRDYYEVLDVPRTATDRQIKKAFHKLAMKYHPDKNKSPEAEVNFRAIAEVAYEVLSDAEKRRQYDEFGHQAFEPDHGDGTDFGQQFFTFNFDDFFRGFQFDDDIMYEMPGEDWDSREEFVDAQKHEGQSYFNGEFGHHDFYDLFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZA4</accession>
    <name>W5MZA4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Nuclear receptor subfamily 2, group C, member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630783.1">
        <property type="nucleotide sequence ID" value="XM_006630720.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015202856.1">
        <property type="nucleotide sequence ID" value="XM_015347370.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5MZA4"/>
    <dbReference type="FunCoup" id="W5MZA4">
        <property type="interactions" value="1475"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013713"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013742.1">
        <property type="protein sequence ID" value="ENSLOCP00000013713.1"/>
        <property type="gene ID" value="ENSLOCG00000011160.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683376"/>
    <dbReference type="KEGG" id="loc:102683376"/>
    <dbReference type="CTD" id="7182"/>
    <dbReference type="eggNOG" id="KOG3575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158393"/>
    <dbReference type="HOGENOM" id="CLU_007368_16_2_1"/>
    <dbReference type="InParanoid" id="W5MZA4"/>
    <dbReference type="OMA" id="IHWARSI"/>
    <dbReference type="OrthoDB" id="10024684at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011160">
        <property type="expression patterns" value="Expressed in brain and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of embryonic development"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="Nuclear receptor subfamily 2 group C member 1"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="Retinoid X Receptor"/>
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        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
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        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
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        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
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        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
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        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
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    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="147"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="373"/>
            <end position="615"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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            <dbReference type="PROSITE" id="PS51030"/>
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            <dbReference type="PROSITE" id="PS51843"/>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013713.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="628" mass="68860" checksum="F2152E91E0CAC8D9" modified="2014-04-16" version="1">MATNMEVLAQQIVDTEQVAEVQTVQTSTPDSSVMSSSPQRIQIISADSAVTSPQRIQIVTDQQTGQKIQIVTAVDPSSTSKQQFILATPDGAGAGKVILASPETSNTKQLIFTTTDNIVPGRIQIVTDAASVERLLGKADVGRSQPVEYCVVCGDKASGRHYGAVSCEGCKGFFKRSVRKNLTYSCRSNQDCIVNKHHRNRCQFCRLKKCLEMGMKMESVQSERKPIDLPREKPANCAASTEKIYIRKDLRSPLIATPTFITEKDGSRSSLLDPGMLVNIQQPLIQADGTLLLATDSKAESSQGDLGTLANVVTSLANLNESLNNGDASEMQQEDQSASEITRAFDTLAKALNPTDTTAVQNLTEGTDTGGGASIQVISRDQSTPIIEVEGPLLTDTHVSFKLTMPSPMPEYLNVHYICESASRLLFLSMHWARSIPAFQALGQDCNTSLVRACWNELFTLGLAQCAQVMSLSTILAAIVNHLQNSIQDDKLSGDRIKLVMEHIWKLQEFCNSMAKLEIDGYEYAYLKAIVLFSPDHPGLSSTSQIEKFQEKAQMELQDYVQKTYPDDTYRLARILMRLPALRLMSSGITEELFFTGLIGNVPIDSIIPYILKMETAEYNSQITGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5MZF7</accession>
    <name>W5MZF7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Apolipoprotein L</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000013766"/>
    <dbReference type="Ensembl" id="ENSLOCT00000013795.1">
        <property type="protein sequence ID" value="ENSLOCP00000013766.1"/>
        <property type="gene ID" value="ENSLOCG00000011203.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVW4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="W5MZF7"/>
    <dbReference type="OMA" id="KMEANNV"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG12"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011203">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APOLIPOPROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000013766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="493" mass="53624" checksum="70C8E61074C7FB8D" modified="2025-10-08" version="2">MEKELGGILEETLAGLAELDPVLEAVGKLSVTSLPLFTDEYHQAVELNAPDVPVPIAITILVAPFLLHFQRRAKDLFLPDLANIEMLVPQLETYLQNAEELCKKLKIMRETRTDEEQQEFLKSSSGISALESIQQPVFPHLHSDLTTESLQEMYDHLEQLNKIREDEHLRITFLFKDNALKFIGLLSARNSRMRKYLQELEETANLLDKVEKGTAISSVTGSSVGLVSGALSIAGLALAPVTAGLSLPLFLSGVVLGVTSAVGSVATGVTGIAMNIQQVKRFQEVSKNYAKDAAAITEFIHSVASNKGQGVDIDNANVDYKAVIEAAKMVVGLVSIGKGIDTIVDCASAVNLLKTERLVASAAKVALQEGKAARSVPGAVDIVDIGRAAKGTPLALSKAARVGFIAANAVFMGLDLFIICKDSVGLAQGKKNEVGQEIRRKVRLLRDELDCWEQIHSSVCRGIWRFRKSQEILQRSLYPSNNRKTNKNVCELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N0W4</accession>
    <name>W5N0W4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stabilizer of axonemal microtubules 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031535">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N0W4"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014302.1">
        <property type="protein sequence ID" value="ENSLOCP00000014273.1"/>
        <property type="gene ID" value="ENSLOCG00000011617.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SMWE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007252"/>
    <dbReference type="HOGENOM" id="CLU_047658_0_0_1"/>
    <dbReference type="InParanoid" id="W5N0W4"/>
    <dbReference type="OMA" id="MKEDYRA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011617">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05217">
        <property type="entry name" value="SAXO1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="482" mass="55076" checksum="DC5B012E8DC226D5" modified="2014-04-16" version="1">QVTMKTWCICDICTCGWHRCPHLPTELFSSDTASFLTEYNEKFCPYGFSAVRQPHRAKDELQIHTGKMENTTTFRADYVRHKLSKAPTKTVKEYIQPEGDMMLDTTYSRAYISHPTQYPVPAVKPAMYRPSSANMDVIPTYKDDYRAWEIPKQEVIRTASNLKLCSAKFSNTTAFQDDYGARLPVKSEKSYKPVQEVKEPMPFDDLTNYKVEYVCHAVQPRLRTEKKAYKPQNAPFDGLTTHKRDYKGLIGAQTRPVKPKLAWQNTAPHFEASTEFQDKYKLWPVQPSQPRKSVKHTPPEGAMDTISTTHADFVGHQVQPPRPARPPVQEWKKGEPFDAQSTMKEDYRPWEIKKRSPIVQVGEIEKPAGPFESTTTFQAHYVPHTLARATNYKLMPTMLQSQPFNADTTYSTSYTAKEIRVCPASFPVPPGFEHEETDPLGHKLYRTISTRDNHTPAAVTKTTSRPNHNRTPSAKKEPARSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5N100</accession>
    <name>W5N100_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ladybird homeobox 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017395">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N100"/>
    <dbReference type="FunCoup" id="W5N100">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014309"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014338.1">
        <property type="protein sequence ID" value="ENSLOCP00000014309.1"/>
        <property type="gene ID" value="ENSLOCG00000011648.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164611"/>
    <dbReference type="HOGENOM" id="CLU_086390_0_0_1"/>
    <dbReference type="InParanoid" id="W5N100"/>
    <dbReference type="OMA" id="THPKHGL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011648">
        <property type="expression patterns" value="Expressed in bone element and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000098">
        <property type="entry name" value="Transcription factor LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051892">
        <property type="entry name" value="LBX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="217"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="270" mass="29847" checksum="34F3ECC5D442331E" modified="2014-04-16" version="1">IMTSKEDAKGSSVEERRRSPLDHLPPPANSNKPLTPFSIEDILNKPSVRRSYTICGTAHLISAAEKHPPPGLPLSSRALLTQTSPLCALEELASKTFKGLEVSVLQAAEGRDGMTIFGQRNTPKKRRKSRTAFTNHQIYELEKRFLYQKYLSPADRDQIAQQLGLTNAQVITWFQNRRAKLKRDLEEMKADVESAKALGSVPLEKIAKLADLEKSAAGTLGNSRSESPTLSSHGHETPNKLQMSPSSPFTDHATSQECSEDEDVEIDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1B8</accession>
    <name>W5N1B8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7</fullName>
            <ecNumber evidence="13">1.14.11.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">JmjC domain-containing protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Jumonji domain-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as a Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer; disulfide-linked. Interacts with DRG1 and DRG2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.63" evidence="13"/>
    <dbReference type="EMBL" id="AHAT01019087">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1B8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014456.1">
        <property type="protein sequence ID" value="ENSLOCP00000014427.1"/>
        <property type="gene ID" value="ENSLOCG00000011726.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102695533"/>
    <dbReference type="KEGG" id="loc:102695533"/>
    <dbReference type="CTD" id="100137047"/>
    <dbReference type="GeneTree" id="ENSGT00900000141196"/>
    <dbReference type="HOGENOM" id="CLU_016785_6_0_1"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011726">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106155">
        <property type="term" value="F:peptidyl-lysine 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000059">
        <property type="entry name" value="jmjC domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="17">
        <location>
            <begin position="130"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="321" mass="37141" checksum="B3403A03EA9649E9" modified="2014-04-16" version="1">MVVMADGLDVLKTCLKHFSEEARELYLNKDVPYLECPPSPLEFYREWIGPNKPCIIRNAFNHWPALCRWSPAYLRERVGSKVISVAVTPNGYADAVCGDRFVMPEERHMPFSSVLDIMERKVESEGVFYVQKQCSNLTEELPELTEDLETHIPWMSEALGKKPDAVNFWLGDSAAVTSLHKDHYENLYCVITGEKHFILIPPSDRPFIPYELYQPATFSQGEDGTFEVVDEQNADKVPWIPVDPLSPDYERYPEYRQARPLSCTVRAGEMLFLPALWFHHVRQSHGCIAVNFWYDMEFDIKYNYFQLLDSLSRAVGQLEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1I7</accession>
    <name>W5N1I7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DnaJ homolog subfamily C member 21</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01017226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1I7"/>
    <dbReference type="FunCoup" id="W5N1I7">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014496"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014525.1">
        <property type="protein sequence ID" value="ENSLOCP00000014496.1"/>
        <property type="gene ID" value="ENSLOCG00000011784.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047097"/>
    <dbReference type="HOGENOM" id="CLU_009539_1_0_1"/>
    <dbReference type="InParanoid" id="W5N1I7"/>
    <dbReference type="OMA" id="RANHEES"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011784">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000046">
        <property type="entry name" value="dnaJ homolog subfamily C member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="531" mass="61253" checksum="CD59EE2ED1A51377" modified="2014-04-16" version="1">MKCHYEVLGVRRDATDDDLKKAYRKLALKWHPDKNLENAEEAAEHFKLIQAAYDVLSDPQERAWYDNHREALLKGGVSGEFEDDSIDLLQYFTVTCYSGYGDDEKGFYTVYRKLFESIAKEEMEYSKEEEEGEFPTFGDSQSDYDTVVHQFYGYWQSFCTWKNFAWKEEYDTRQASNRWEKRAMEKENKKLRDKARKERSELVRQLVAFLRKRDRRVQAHKKMVEEQNAEKAKKVEELRRRQKLSQAKLAENYKEQSWAAMSELEKELQQMEAQYEKEFGDGSESEEEEAEVPEGGEEPGDTLTDGADELADYFDSLYCPACNKSFKTDKAMKNHEKSKKHREMVILLRQQLEEEDLVLNQDSEDSGENAKEENEEDLNDNPKQKPSKKQKKKKRQQKLSPAAHEEAPQLETEDTESTLPSEPQAIHQAETAAGDLKQEDPAPSAPKSSGKAKAKKVGKETKKGNKPLGGTDQSLEKEINLRCVTCQFEFSSRNKLFDHLKTTGHATALYSLSAPCGSGSGKSKKEKRKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5N1Y4</accession>
    <name>W5N1Y4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01023018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N1Y4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014643"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014672.1">
        <property type="protein sequence ID" value="ENSLOCP00000014643.1"/>
        <property type="gene ID" value="ENSLOCG00000011906.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156832"/>
    <dbReference type="HOGENOM" id="CLU_055524_6_1_1"/>
    <dbReference type="InParanoid" id="W5N1Y4"/>
    <dbReference type="OMA" id="MVSCFIC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG15"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000011906">
        <property type="expression patterns" value="Expressed in testis and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF511">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="228" mass="24885" checksum="5FBF26117E477245" modified="2014-04-16" version="1">MAYGGSCCLLQVSGIALIIVGEISRTSYNEFESFTGGGLSHIAVLLIVVGVAISITAFLGCLGAWLDNSILLGLFTGILILILTLEIVAGILFYVFRAKVESKITKKAKEVIKKYDIRDRAIIDDIQDQFHCCGAENYTDWFVSGGWKNHRAVPDSCCRLESEDCGHIITSDNIYLKGCVKSIKEFLKKNLVWIGAACIALGLVEILGVVLGVCLLNNILRRGSYDVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2C6</accession>
    <name>W5N2C6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Rho GTPase-activating protein 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Rho-type GTPase-activating protein 24</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with FLNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01017244">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01017245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006627206.1">
        <property type="nucleotide sequence ID" value="XM_006627143.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015195664.1">
        <property type="nucleotide sequence ID" value="XM_015340178.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2C6"/>
    <dbReference type="FunCoup" id="W5N2C6">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000014785"/>
    <dbReference type="Ensembl" id="ENSLOCT00000014814.1">
        <property type="protein sequence ID" value="ENSLOCP00000014785.1"/>
        <property type="gene ID" value="ENSLOCG00000012018.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687408"/>
    <dbReference type="KEGG" id="loc:102687408"/>
    <dbReference type="CTD" id="83478"/>
    <dbReference type="eggNOG" id="KOG4270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183015"/>
    <dbReference type="HOGENOM" id="CLU_020795_0_0_1"/>
    <dbReference type="InParanoid" id="W5N2C6"/>
    <dbReference type="OMA" id="QNAMKCG"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012018">
        <property type="expression patterns" value="Expressed in bone element and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035021">
        <property type="term" value="P:negative regulation of Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900028">
        <property type="term" value="P:negative regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035313">
        <property type="term" value="P:wound healing, spreading of epidermal cells"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04390">
        <property type="entry name" value="RhoGAP_ARHGAP22_24_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000015">
        <property type="entry name" value="rho GTPase-activating protein 25 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF19">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="19"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="20">
        <location>
            <begin position="135"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="351"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="558"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="654"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="381"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="621"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070253"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000014785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="750" mass="84703" checksum="07129C7C3D349DAB" modified="2014-04-16" version="1">MDEQSGSNNSPQQGRSRQNVIRCGWLRKQGGFVKTWHTRWFVLKGDQLYYYKDEDETKALGAIFLPGNRVIEHANNGDEPGKFLFEVIPGADRERMTANHETYLLMASTQNDMEDWVKTIRRVIWAPFGGGIFGQKLEETVRYERRFGNKMAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDCNTDVHTVASLLKLYLRELPEPVIPFAKYEEFLTCTKLFNKDEESGMKELMKQVKSLPPVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKIEDPVAIMEGTVLVQQLMSVMIGKHDMLFPKDEENEMVDEVSNNNIEAQKKYTTGQLQNKENNNTKEVSVRQCSWDKPDSPQRNSVDNGSPTSLGANRSSSPRNSLSSKFDITRSPPLMVKKNPAFSKGSGIVTNGSFSSSSLDMQEKSQTLPNGTLQARRTSSLKGTGTKMGTSGVPNGGVRMGISSTDVMNATLNTRNGVWMPNGYVTMRDNKQKDSLNEQSTQQNRLSTYDNVQQQYSSVNCEDKQSVDSATWSTSSCEISLPDNSNSCRSSTTTCPEQDFFGGNFEDPVLDEPSRDDISQPGEFDSKFDRQSGGGQSSRATSSSDNSETFVANNTTSNHSALHSLVASLKQEMLKQKTEYETRLKSLENRNLDLEAEMVSLHEELDQERKKYTMVEIKLRNAERAKKDAEKRNETLQKEMEQFFSTFGELTVEPRRPERGNTIWIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5N2X3</accession>
    <name>W5N2X3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin-12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01002603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002604">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01002605">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N2X3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000014982"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015011.1">
        <property type="protein sequence ID" value="ENSLOCP00000014982.1"/>
        <property type="gene ID" value="ENSLOCG00000012173.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG9"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012173">
        <property type="expression patterns" value="Expressed in brain and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
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    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
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    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
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    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
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    </dbReference>
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        <property type="term" value="P:cell migration"/>
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        <property type="term" value="P:cell morphogenesis"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
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    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
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    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.60:FF:000097">
        <property type="entry name" value="cadherin-12 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF323">
        <property type="entry name" value="CADHERIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 15 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867095" description="Cadherin-12" evidence="19">
        <location>
            <begin position="22"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="72"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="153"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="262"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="377"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="481"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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        </source>
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        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="779" mass="88418" checksum="A0156B7D79139741" modified="2014-04-16" version="1">MNCSAWLPMLIAFSMMGCCVTNETRTKLCLKTPSLNKEQYISHRRLRRGWIWKQFFVPEEDPAPRTIGQLKSDYDKVNFSIKYILSGEGAGDVFRIDEDTGEIYTMKKLDREKKSSYILRAQAIDRLSMQPVEPESEFIIKVQDINDNVPEFQNEPYVATIAEMCPTGTTVIQVTATDADDPSYGNNARLIYSILQGQPYFSVEPTTGTIVTSWPDMDREAKEQHLVIVQVKDMMGLRGGFSATTTVTINLSDVNDNGPKFQHKFYSFPIPESAPVGTIIGRIMAEDRDVGLNGMMNYTIEETDSTGVFEILTDPNTQEGVIILQKALDYENRRRFTISVEAINAYIDIRFLTFNQFRDKTTVKVTVENVDEPPLFFPHEYYWEILENTAVGTAIGRVNAYDRDAANNPVRYYFDERTNMNKMFSIDQYNGTITIAKPLDRETAHWHNLTVSARETRENYLFSKAQITIQVMDVNDNAPQFSKQYTPYVCEGTQSGEMMQSIQRVQRTKNNGNVQIIISLCNIMYFFLTKIVSDNTAGVLTRRSSFSRKDRAYYYLPIVIVDNGTPALTSTTTLTIEVCSCYPDGNCRSNGVEAFALSIGLSIETLIAISSCLLIIVVLSLLILAVKRHKKKQLGDEKWEEVAENVVCYNEKGYGEEDTHAIVPVILRSNPKQRNRRTHKEEVAASIRMSLRQSFLIGPEDEVFRQFILERLFEADTDPYAPPFDYLHTFAYEGTGSAAGSLSSLGSWPSEQNYDYICDSGPCFKRLAYLYVGGHEETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3A9</accession>
    <name>W5N3A9_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.1.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Short-chain 3-hydroxyacyl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion. Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3S)-3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30799"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Interacts with GLUD1; this interaction inhibits the activation of glutamate dehydrogenase 1 (GLUD1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="AHAT01017715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006629879.1">
        <property type="nucleotide sequence ID" value="XM_006629816.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3A9"/>
    <dbReference type="FunCoup" id="W5N3A9">
        <property type="interactions" value="1132"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015118"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015147.1">
        <property type="protein sequence ID" value="ENSLOCP00000015118.1"/>
        <property type="gene ID" value="ENSLOCG00000012288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686410"/>
    <dbReference type="KEGG" id="loc:102686410"/>
    <dbReference type="CTD" id="3033"/>
    <dbReference type="eggNOG" id="KOG2304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159984"/>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_1"/>
    <dbReference type="InParanoid" id="W5N3A9"/>
    <dbReference type="OMA" id="MAHPMGP"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012288">
        <property type="expression patterns" value="Expressed in intestine and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="3-OHacyl-CoA_DH"/>
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            <begin position="24"/>
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            <position position="165"/>
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            <dbReference type="Pfam" id="PF02737"/>
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            <dbReference type="Proteomes" id="UP000018468"/>
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    <sequence length="309" mass="33738" checksum="FDA39C39F7C1BB81" modified="2014-04-16" version="1">MAFVTHQIIRSLSSSAVRSGVIKHVTIIGGGLMGAGIAQVAASTGHSVVLVDQSEDILKKSTKGIEDSLKRIAKKKFADKPEAGAEFIQKVLKNVTTSSDAASVIHSTDLVLEAIVENLKIKQDLFSGLDKFAPDHTIFASNTSSLPITDIANSTSRQDRFGGLHFFNPVPLMKLVEVIRTPMTNQKTFDALLEFSKALGKHPVSCKDTPGFIVNRLLVPYMMEAIRLHEKGHGSKEDIDVAMKLGAGYPMGPFELLDYVGLDTSKFIIDGWHERDPDNPLFAPSPMLNQLVEQGKLGKKTGEGFYKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3D8</accession>
    <name>W5N3D8_LEPOC</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDCD4 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015147"/>
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        <property type="protein sequence ID" value="ENSLOCP00000015147.1"/>
        <property type="gene ID" value="ENSLOCG00000012312.1"/>
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    <dbReference type="eggNOG" id="KOG0403">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015948"/>
    <dbReference type="HOGENOM" id="CLU_025354_1_0_1"/>
    <dbReference type="InParanoid" id="W5N3D8"/>
    <dbReference type="OMA" id="TRTHPQY"/>
    <dbReference type="OrthoDB" id="414546at2759"/>
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        <property type="component" value="Linkage group LG5"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:atrioventricular valve development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 4"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="MI" evidence="10">
        <location>
            <begin position="164"/>
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    </feature>
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        <location>
            <begin position="327"/>
            <end position="450"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="38"/>
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    </feature>
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        <location>
            <begin position="40"/>
            <end position="50"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="58"/>
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        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005497"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014414"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="470" mass="52117" checksum="1D43935EA15A6170" modified="2014-04-16" version="1">MATEIEAWLNANHVAAEALSDSSPSGEDEKVETTKLDNEMNGNWITSPTVTIHEARVKAKAKRRLRKNSSRDSGRGDSLSDNGESTRSPLVTPTSPKSKLLDRKSRSGKGRGLPKKGGAGGKGVWGPPGEVYDLEEIDVRDPNYDEDQENCVYETVVLPLDEGAFEKTVTPIVQEYFEHGDTNEVVELLSELNLGEMKSGVPLLAVSLALEAKASHRELTSQLLTDLCGKVLSPDDVEKSFDRLLRELPDLVLDTPGAPQLVGQFIARAVGDQILSRNYIESYKGKVDCEHARAALDRAAVLLKMSKGGLRIENVWGTGGGQRPVKQLIKEVNLLLKEYLLSGEVMEAERCLRELEVPHFYHEFVYEAIVMVLESKGETTLKMILQLLKFLWESSVITVDQMSRGFQRIYAEIPEINIDVPCAYLVLEQFVEQSFSAGIIGKRLRDLCPCRGRKRFVSEGDGGRLKLESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5N3F0</accession>
    <name>W5N3F0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01016650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006628532.1">
        <property type="nucleotide sequence ID" value="XM_006628469.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N3F0"/>
    <dbReference type="FunCoup" id="W5N3F0">
        <property type="interactions" value="1963"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015159"/>
    <dbReference type="MEROPS" id="T01.975"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015188.1">
        <property type="protein sequence ID" value="ENSLOCP00000015159.1"/>
        <property type="gene ID" value="ENSLOCG00000012319.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102690182"/>
    <dbReference type="KEGG" id="loc:102690182"/>
    <dbReference type="CTD" id="5686"/>
    <dbReference type="eggNOG" id="KOG0176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074958"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_2_1"/>
    <dbReference type="InParanoid" id="W5N3F0"/>
    <dbReference type="OMA" id="RSMIDHA"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012319">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000019">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="241" mass="26405" checksum="AAD4776A38D27CA9" modified="2014-04-16" version="1">MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTAEGVCLAVEKRITSPLMEPSSIEKIVEIDSHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLYHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKDAIKSSLTILKQVMEEKLNATNIELATVEPGKTFHMYTKEELEEVIKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N451</accession>
    <name>W5N451_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Katanin p80 subunit B-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01019043">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01019044">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N451"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015410"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015439.1">
        <property type="protein sequence ID" value="ENSLOCP00000015410.1"/>
        <property type="gene ID" value="ENSLOCG00000012512.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686614"/>
    <dbReference type="KEGG" id="loc:102686614"/>
    <dbReference type="CTD" id="79768"/>
    <dbReference type="eggNOG" id="ENOG502QT0F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012351"/>
    <dbReference type="HOGENOM" id="CLU_079657_0_0_1"/>
    <dbReference type="InParanoid" id="W5N451"/>
    <dbReference type="OMA" id="VVLHWVQ"/>
    <dbReference type="OrthoDB" id="8754475at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012512">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042404">
        <property type="entry name" value="KATNBL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14682">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14682:SF1">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="4">
        <location>
            <begin position="148"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="307" mass="34629" checksum="52AC0522DAB31D60" modified="2014-04-16" version="1">MASGEHDAEVKDFQHLEEALPNELLNQKMLSSGKKNMKEVDFFKLEEINKKRSSVGCTGQNSTKVKRVVSSKRKTRLVFVSSGGRRRQPLASRSCDMANKENELACAGSLHERLYNDDHCGFPLNSAGSTRMEGAGSKYSDYFAEISKDHDTMTQVLFGRNLRLNVALTLWRRNTSELVAYLIRIQDTGVLVDCLPVITKSLQDEKSSISLGCCVDLLPLVKNVLKSKYEEYLIVGLLWVQSVIKKWWPELSANGRNARDSHSEDRNIGIMKQQLRELWEEGKHLCCVPGNTGDIAKTVESYLSQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4E3</accession>
    <name>W5N4E3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CXXC-type zinc finger protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N4E3"/>
    <dbReference type="FunCoup" id="W5N4E3">
        <property type="interactions" value="897"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015502"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015531.1">
        <property type="protein sequence ID" value="ENSLOCP00000015502.1"/>
        <property type="gene ID" value="ENSLOCG00000012594.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QQVJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="CVNKLKC"/>
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        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012594">
        <property type="expression patterns" value="Expressed in brain and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
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    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:Wnt signaling pathway"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040388">
        <property type="entry name" value="CXXC4/CXXC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419:SF1">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="10">
        <location>
            <begin position="223"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="289" mass="31376" checksum="3B5EAAADF5DE865D" modified="2014-04-16" version="1">MSNINNALCVENGQSAEPSILQKDNLQESGLNPVLDYNAEMERYRSFANFYKTNGAFPQTAKIARITTPIFPSARIGVSPWNCDNAMLWGRKSAAINSNRTSMHRNDSQRPGKPGVPPETLQMANNNFLTTLSPEHCRPLAGECMNKLKCGAAEAEIMNLPERVGTFSAIPALGGISLPPGVIVMTALHSPAASAAVTDSAFQIANLADCPQNNSSASSGNPAKKKRKRCGVCAPCRRLINCGVCSSCRNRKTGHQICKFRKCEELKKKPGTSLEYTILVNARVIRFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5N4Q5</accession>
    <name>W5N4Q5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nuclear pore complex protein Nup155</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">155 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleoporin Nup155</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01024764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01024766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000015614"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015643.1">
        <property type="protein sequence ID" value="ENSLOCP00000015614.1"/>
        <property type="gene ID" value="ENSLOCG00000012677.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016532"/>
    <dbReference type="HOGENOM" id="CLU_000429_0_0_1"/>
    <dbReference type="InParanoid" id="W5N4Q5"/>
    <dbReference type="OMA" id="SWAPFQK"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012677">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Nuclear pore complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear pore complex protein Nup155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1880">
        <property type="entry name" value="Nucleoporin, helical C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042538">
        <property type="entry name" value="Nucleoporin_Nup155_C_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="21">
        <location>
            <begin position="73"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="20">
        <location>
            <begin position="658"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="988"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="988"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1394" mass="154936" checksum="010A12617E9CB6B3" modified="2014-04-16" version="1">MPSSLGASNPAAALQEALENTGRLIDRHLQDDRCFPDLSELLNVPAHNMPSLSGMSDMDYPLQGPGLLTVPSLPEISPVRRVPLPPELVEQFSHMQCNCMMGVFPEISRAWLTIDNDIFMWNYEDGGDVAYFDGLSETILSVGLVKPKEGIFQPHIQYLLVLATPVDVVILGLSFPGSQAGALNDSMSSGMQLLPDPLYSIPTDNTYLLAVTSTDLGRIFLAGKDGCLYEIAYQAEAGWFSQRCRKINHSKSTLSFLIPSVLQFTFSEDDPIVQIAIDNSRNTLYTRSEKGVIQVYDLGVDGQGMSRVAGMSQNSIVSAAGNIARTIDRSVFKPIVQIAVIERSESSDCHLLAITHAGVRLYFSTTPFSPPNARHTSARPGMLVLVHVRLPPGFSASATLQKPSKVHKALYSKGVLLMAASETEDGDILWCINHDSFPFKKPLMEAQMTSTVDGHTWSLCAVEESRAVKVVTPLNRDLVPHTDSPVVVQQHNIPPKKFVLLSAKGSHIFHKLRPVDQLRHLLVSCAGGDGEEIERFFKLHREEQACATALILACSSAAYDREVSLWASRAFFRYGGEAQMRFASALSAPVSSVGPMLSSPVPGSPLPISSPVPNPSFLATPAPGSYPPSVSTPFVQAPSMGQAGAPITAMAGPEVVFSGKHNGICIYFSRILGNIWDGSLATEKTISTGNQQVTIMESSVPSSVLESVLQELRGLKDFLDKNSQFSPGSLGAVSFSSPGNLQQRLLGFMRPDGGSSQQVQQELQRKYHTEAQAYEKISLQGIQQLVHHTCQTLALWKLLCDHQFGLILNDLPKEFQEQVKVSSFKDIVIRGRELTGALITSLINCYIKDSASVDAISCHLRETCPLLYSSDDSVCSKANELLQSTRQIQNKAEREKMLRESLRLYQQISHQTDLPHVCAQYRQGSVRFYEGVVELCLTAADKKDPQRLGPHFYKNGEPEEDAAGQQAFQERLNSYKCITDAMQDLVNQSKAAPQSPSVPKQPGPPVMSSDPNMLSNEDAAAHFEQMIGLAQRSKDELFHIALYNWLIQADLTDKLLEVNSPYLEEHLMRMMKQDQNKVRYMDLLWRYYEKSRNFSKAAHVLARLADMHSTEISLRQRLEYISRAILSAKSSSCASSQASDGEFLHELEEKMEVVRIQVQIQETLSRQYSHHPSVQGAISQLDSELMDITKLYGEFADHFRLSECKLAIIHCAGHSEPMLVHTLWQEIVEKELSDSVAMSPADRMRALSLKLVSLGKIYAGTPRYFPLEFLVKFLEQEVCRLSWDVGFVTYTMQEIGVQLPRLLEVYDQLFRARDPCWQRLKRPLHLVECIHVLLSGYVEDPSRVPSYDRRRFTTFCLDCICGYLVELQSLSPNSALQQLIGNFKSLQAKVEKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N585</accession>
    <name>W5N585_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc finger CCHC domain-containing protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TRAMP-like complex RNA-binding factor ZCCHC7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01001916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047748.1">
        <property type="nucleotide sequence ID" value="XM_069191647.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_069047751.1">
        <property type="nucleotide sequence ID" value="XM_069191650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N585"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015794"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015824.1">
        <property type="protein sequence ID" value="ENSLOCP00000015794.1"/>
        <property type="gene ID" value="ENSLOCG00000012834.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102694476"/>
    <dbReference type="KEGG" id="loc:102694476"/>
    <dbReference type="CTD" id="84186"/>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183041"/>
    <dbReference type="HOGENOM" id="CLU_029787_0_0_1"/>
    <dbReference type="InParanoid" id="W5N585"/>
    <dbReference type="OMA" id="YEDDLYH"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012834">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000020">
        <property type="entry name" value="Zinc finger CCHC domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="379"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="487"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="527" mass="60485" checksum="A20D14D20BCC90B7" modified="2014-04-16" version="1">MFAGYESMEEYEDELYKEPGQSSDSELDSEVEFHLYSQVHYASRAGDGEVEDKVEENGAVDRGQEEEVISITSGCEPEVITLSDSTEEESICVTKGSKMDGFSKALRKRACPSSSTPLPPRKGQRETAAEQAETLSDSEDSDCVESWMILGRGQLAEDRSIQLNLKDHSRESWGSGSGEDNWTISDKDIEAQICNKRDRLRSVNRYYKPNKNVTCRSCGKNGHLSKNCPTPKKAPVCILCAEPGHLQRSCPLKHCANCNRPGHESEECIDRAFWNKQCHRCGMRGHFFDDCPEIWRQYHLTTSSGPIVIPDDKEKQKSPAYCYNCSRKGHLGHECKQKRMFNNTFPTLPFITYYDTKPDIEGTNHRVWKRAQELYGAGLLREEPASKRRKTATVEEKKERGEAQPPKPKQSKQPKQQNKKTKTWPEKRKELWLLKQQRRKARQEVVEDTEDFPRGPKKTSSGVPVHPKKRSRSPGLFSTLSQPSKEKPRKLRPKKKAKRRQGRLHDNSFPVDEDLFLIKQKVRRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5N5Q0</accession>
    <name>W5N5Q0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NOP2/Sun RNA methyltransferase family member 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01001958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001959">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001960">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001961">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01001962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N5Q0"/>
    <dbReference type="SMR" id="W5N5Q0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000015959"/>
    <dbReference type="Ensembl" id="ENSLOCT00000015989.1">
        <property type="protein sequence ID" value="ENSLOCP00000015959.1"/>
        <property type="gene ID" value="ENSLOCG00000012956.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2360">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157352"/>
    <dbReference type="InParanoid" id="W5N5Q0"/>
    <dbReference type="OMA" id="QIPTQHF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000012956">
        <property type="expression patterns" value="Expressed in testis and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042620">
        <property type="entry name" value="NSUN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14663">
        <property type="entry name" value="METHYLTRANSFERASE NSUN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14663:SF2">
        <property type="entry name" value="METHYLTRANSFERASE NSUN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="199"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="597"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="628"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000015959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="713" mass="78858" checksum="9D0BD60204BC353F" modified="2014-04-16" version="1">PRSESNMEPRSQSPEIGAEISSFGDLSISHKVDPSKKSPAVSAESGGYPDYVYVHAAAIFFTIHVEKPVDRRLVRYGDQISFSVPEAKNEIAQHYAHELAFNALKYQDLLEDIMIDSCFYLSQSIPDDLTSLVVVMLYDFQDRKFLPRACPEEEEIEEVRDVEKCLLRYRTKLAAALARCRIKHNLLTIDYILPESVRKKQERASTLPLYAWVNTMKTSLEDVCCTLEKEGFSEVKSISQLGGLTFCLDPHCQDLLVFPPHLKADLYRTKLLTEYKLIIQDKSRSLAAHSVKALLTDDDDVIMANLTSGYTIAHMAALSTLSTGRVWVCGVSQAREELQEILSHMECKNVKLLPESFTDIEPTDPRLQKAKVILVMPQCSATGVSNPVEFILNDSGDAGLLQDLCQGSISEDKLNTLVRQQLQDLIHAVKFPKAQAVVYCTCSVHPEENEGVVRKALLFKPEGTKAQPYRLSPPVLPLCCMSEVDNARENYFRLGPCERANGCFIAVLTREKDSSQTVTVKDVLARAAAKGLLEGIDTGKPAKKERRKAKASVLPSSSASASQARIAEFLSRERKTTSSSSSLLLPAKACTSSHALLRAGQRARTPKPTPSSLPAKKNGSKPPPAGRPPERRAHPPRARPEERASALKPVELVLPPVMFPGCGQSRSPLPLSFFRWKGTSGHSQTSLSPSSLSVSRHREALRSGVVLHPRPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>W5N6H2</accession>
    <name>W5N6H2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform</fullName>
            <ecNumber evidence="8">2.7.1.137</ecNumber>
            <ecNumber evidence="6">2.7.1.153</ecNumber>
            <ecNumber evidence="7">2.7.1.154</ecNumber>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Phosphoinositide-3-kinase catalytic gamma polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Serine/threonine protein kinase PIK3CG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">p120-PI3K</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="29">Heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with GRK2 through the PIK helical domain. Interaction with GRK2 is required for targeting to agonist-occupied receptor. Interacts with PDE3B; regulates PDE3B activity and thereby cAMP levels in cells. Interacts with TPM2. Interacts with EPHA8; regulates integrin-mediated cell adhesion to substrate. Interacts with HRAS; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="8"/>
    <dbReference type="EC" id="2.7.1.153" evidence="6"/>
    <dbReference type="EC" id="2.7.1.154" evidence="7"/>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="AHAT01029321">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N6H2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016231"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016261.1">
        <property type="protein sequence ID" value="ENSLOCP00000016231.1"/>
        <property type="gene ID" value="ENSLOCG00000013171.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165924"/>
    <dbReference type="InParanoid" id="W5N6H2"/>
    <dbReference type="OMA" id="QEIHYNT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013171">
        <property type="expression patterns" value="Expressed in intestine and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036092">
        <property type="term" value="P:phosphatidylinositol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010595">
        <property type="term" value="P:positive regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035022">
        <property type="term" value="P:positive regulation of Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003376">
        <property type="term" value="P:sphingosine-1-phosphate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000010">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000087">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.770:FF:000008">
        <property type="entry name" value="Si:rp71-17i16.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045580">
        <property type="entry name" value="PIK3CG_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF99">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PI3K_C2"/>
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        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19710">
        <property type="entry name" value="PIK3CG_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PI3Ka"/>
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    </dbReference>
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        <property type="entry name" value="PI3Kc"/>
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        <property type="entry name" value="ARM repeat"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2_PI3K"/>
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        <property type="entry name" value="PI3_4_KINASE_1"/>
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    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0254">Endocytosis</keyword>
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        <location>
            <begin position="219"/>
            <end position="307"/>
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        <location>
            <begin position="355"/>
            <end position="525"/>
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            <begin position="528"/>
            <end position="710"/>
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            <begin position="783"/>
            <end position="1066"/>
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            <begin position="532"/>
            <end position="557"/>
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            <begin position="534"/>
            <end position="546"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
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    </evidence>
    <sequence length="1091" mass="125260" checksum="22BA193887DFEF56" modified="2014-04-16" version="1">FKIFTYGLPLALNNSERQKTKYIRNSDKAVSPQASVPDNILTFACVLPTKRGINVSNEVLIVQLPAQENVRQLRLHICVQAQEKGHHPDICHVLDPECYQLLYTRGPDWCEIYDDYQILRTLEAVRYWHQQGIKMGKIFVKPTSEGKEQGKTFHSNLAYLIGHDLNKTVVDRLDELSFARRKFASPRKIELCNRNPREYVTEPWTASVPLPKDQQTMLNSRIPITFHYNGVSLSVKADLKERPSALIEMFMQKMSDRGSAMEYAASDLTLKVCGREEFLTGEWALADFLWVRQCLKTKEGIHLSVLPVLSFQEDEVRIEDWPLVDSVTGLFNSHRDLTLANKEVDDILMISLWDCNRNFRVKLLGFDIPQFPGKAPQYVYVEAFILHGKNVVSSVRSVPKAFEDEVLWNVWLEFDMLLKNLPRGSKLGFSINGIYIDGNSTKENKSPPAGDYPKGKEKALYFVNLLLIDHRCLLRQGQHTLHMWPFPEQEEEMFTFEADKLSSTTNPDLVNSMAITIILDKYSFPVVLPHSRDSPPSSSSSVSSSLTKDDVSSKPSAVSHKGILQRLREESSQYGSHLLQFLSTINWCDPDTVRDIHWLLHNWDPHDLDISVALELLSIKYADEEVRRMAIKKLERLTNNELLRYLLQLVQTLKVEPYHDSFLARFLIQRALRKSKRVGHYFFWYMRSEVTGSLYFRDRFAVILEAYLMGCGQAMLDGFLKQAQMVDCLHKVATDIKKVFSEKSDLPPNAALILQDMLRSHVLPQNFQVPYDPRIIAGQIVIQLEKCKVMASKKKPLWLEFTCWTSEESPNHTVGIIFKHGDDLRQDMLVIQTLLIMDSIWQEKSLDLNLVPYGCISTGYNIGMIEIVRDATTIATIQRNKGGTTGAFKNDALLEWLKGKCEREETLYQAMERFVTSCAGYCVATYVLGIGDRHNDNIMITEQGNLFHIDFGHILGNTKRFLGVNRERVPFVLTPDFLYVMGRVNKKSSLYFMRFRDTCIRAYLELRSHSSLLVTLFSLMTVTGIPELCCSEDIRYLTEALQVGRNEESARDHFLQQINICEQLGWTVQANWWIHLLVGIKQSSLGKMHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>W5N7V0</accession>
    <name>W5N7V0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CST complex subunit CTC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01030407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030408">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01030409">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N7V0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000016709"/>
    <dbReference type="Ensembl" id="ENSLOCT00000016739.1">
        <property type="protein sequence ID" value="ENSLOCP00000016709.1"/>
        <property type="gene ID" value="ENSLOCG00000013553.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502RBD3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011553"/>
    <dbReference type="HOGENOM" id="CLU_470862_0_0_1"/>
    <dbReference type="InParanoid" id="W5N7V0"/>
    <dbReference type="OMA" id="GDTWTQE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013553">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029156">
        <property type="entry name" value="CTC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042617">
        <property type="entry name" value="CTC1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14865:SF2">
        <property type="entry name" value="CST COMPLEX SUBUNIT CTC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15489">
        <property type="entry name" value="CTC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000016709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="579" mass="63921" checksum="FC2F77227412FFB5" modified="2014-04-16" version="1">MLFPNWNYIPQNAASSGQKVEGYLELCCPPLPILPSPLTLEPEEPLFQGALRPHETARLLKQRFRSPGVRLVVCGQVSLLCPLLNIAGKTFFCFTLSDRERENSVPMLVTNPSLLHWRQCLSVGVSVVISGLRVCTLRRVAAQLVLCPTPQSRLWPLKHFKAPWETPGQAQADTQALLSRWTQEQTAGDTRTQGQTEEQTLTDTETDEQTAGDTETEGQTLTDTETEGQPAGDTQTEGQTLTDTETEGQPAGDTQTEGQTLTDTETEEQRTGDTWTQEQTGDTQKEEQTAGDTWTQEQTAGQMDPEPDRTTGPKTDRQPVSVKPVLRCTAKRSKVIDYKGVISRVLIAEAGLYELDGEVGLCLAYQQVLNYGRGLRPGAEVKLSHVHFMYRPSPHFPPTLLCCCLRTALQIEGFSAVGSEFMPFHHQKSLYVRVLLERELGVSQYLWACHCAATLGERLCPRVVRPGRMRAVAGRFLRSLLADSEAGAPGRRDIYREMLEEPHCCPLVEYSANAPPPCQTPSVSELVSLGESLGWDSLCLPSLLPPTAPHLTSAELNPFLAWSVLSQPAWSLRPPAVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8V6</accession>
    <name>W5N8V6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01007344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8V6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017065"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017095.1">
        <property type="protein sequence ID" value="ENSLOCP00000017065.1"/>
        <property type="gene ID" value="ENSLOCG00000013833.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0578">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182988"/>
    <dbReference type="InParanoid" id="W5N8V6"/>
    <dbReference type="OMA" id="KKGMFTF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013833">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PAK 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="293"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="580" mass="64758" checksum="F410650F6A361729" modified="2014-04-16" version="1">MSDSVDIEEKPPAPPLRMNSNNRDSSSANHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSKETVNNQKYMSFTSGDKSAHGYIAPNALTRLLSSGAPVTLRTLSAAIYDKGTKTASEPPLAPPVSEEEDDDDDEDDDDDNEPPPVIAPRPEHTKSIYTRSVIEPVAPPAPVKEVSTSPVSLVSENTSSTLYRHTDRQRKKSKMTDEEILERLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDRKILKRHMQLWCYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLAKPLSSLTPLIVAAKEAIKNSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5N8Z7</accession>
    <name>W5N8Z7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein FRG1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds to mRNA in a sequence-independent manner. May play a role in regulation of pre-mRNA splicing or in the assembly of rRNA into ribosomal subunits. May be involved in mRNA transport. May be involved in epigenetic regulation of muscle differentiation through regulation of activity of the histone-lysine N-methyltransferase KMT5B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FRG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01002187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006630041.1">
        <property type="nucleotide sequence ID" value="XM_006629978.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N8Z7"/>
    <dbReference type="FunCoup" id="W5N8Z7">
        <property type="interactions" value="1125"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000017106"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017136.1">
        <property type="protein sequence ID" value="ENSLOCP00000017106.1"/>
        <property type="gene ID" value="ENSLOCG00000013868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102683919"/>
    <dbReference type="KEGG" id="loc:102683919"/>
    <dbReference type="CTD" id="2483"/>
    <dbReference type="eggNOG" id="KOG3962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004552"/>
    <dbReference type="HOGENOM" id="CLU_094616_0_0_1"/>
    <dbReference type="InParanoid" id="W5N8Z7"/>
    <dbReference type="OMA" id="IEQWEPI"/>
    <dbReference type="OrthoDB" id="5539371at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000013868">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055120">
        <property type="term" value="C:striated muscle dense body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23338">
        <property type="entry name" value="beta-trefoil_FSCN_FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000031">
        <property type="entry name" value="FRG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="255" mass="28791" checksum="50D678413549FA6F" modified="2014-04-16" version="1">MAEYSRVKSTKLVLKGVKDKSKKKHKDKKRKREDEKEKDDIVGGWWAISNFGEITGSVAIEMDKNAYIHALDTGLFTLGAPHKEDEGPHPPEQFTAIKLSDSRIALKSGYGKYLGINSEGLVVGRSDAIGSREQWEPVFQDGKMALMAANSCFVSYNDEGDIVAKNKAAGEGEMIKFRSCAERETTKKEDIADEDRGNVKHCEINYVKKFQSFQDRKLRVNSDDSSSLKKAREEGNFHETLLDRRAKMKADRYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5N9U3</accession>
    <name>W5N9U3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01029611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5N9U3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017402"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017433.1">
        <property type="protein sequence ID" value="ENSLOCP00000017402.1"/>
        <property type="gene ID" value="ENSLOCG00000014126.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171928"/>
    <dbReference type="HOGENOM" id="CLU_806447_0_0_1"/>
    <dbReference type="InParanoid" id="W5N9U3"/>
    <dbReference type="OMA" id="ASHIGWM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014126">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="37713" checksum="A57CFA2321DADD77" modified="2014-04-16" version="1">MFLPLHNCAAFPRASHIGWMSAHTYSLCVLGNGLVIAADRAFFSFLYLSAPLLLYVEGVFIESARITVYLTAPPGTRAIHSHLETLRVKDRHFSRRTEPCIGTMELDGCGYAAKIFFYLLNVILALEGLLLMVGGSLLVILAQSSQFFQMTGHYEIIMIGLILLIIMGLILIVAIIMVDVAKCSKSIDIYKAYISIISCALGMAIAVGISAYVSRQKVAAQLSDFYTCVYVQYVGNKHNTFLRLSLMLAQRMLDCCGVGGILEPFVQETCPGRDGFWNAITTPTCPNTLSSLDEAIGKIFLGSFIGTAVLLGLAILCCCVFVNSVKLARDRLQQNNLFHLIPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NAF5</accession>
    <name>W5NAF5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acylamino-acid-releasing enzyme</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="EMBL" id="AHAT01014988">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01014990">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015203742.1">
        <property type="nucleotide sequence ID" value="XM_015348256.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NAF5"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000017614"/>
    <dbReference type="Ensembl" id="ENSLOCT00000017646.1">
        <property type="protein sequence ID" value="ENSLOCP00000017614.1"/>
        <property type="gene ID" value="ENSLOCG00000014295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698619"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166103"/>
    <dbReference type="HOGENOM" id="CLU_014230_1_1_1"/>
    <dbReference type="InParanoid" id="W5NAF5"/>
    <dbReference type="OMA" id="WNKDSTH"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG5"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014295">
        <property type="expression patterns" value="Expressed in brain and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000043">
        <property type="entry name" value="acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF1">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="11">
        <location>
            <begin position="86"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="10">
        <location>
            <begin position="523"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000017614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="732" mass="80394" checksum="EB1DD55271C7F41C" modified="2014-04-16" version="1">MESPVMTEPQAVRALFGRYSRYPSPVAAAAGPEEPAPAGGRYISLLTEWTQAQEDRGQRVRFGRQYTLLRAGRTELQALPPGQCCHLQGEVLSSQSPSGSLKAVVRDVACHGAGARHQALEIWNKNGLAKSMDLTSLDKHGRVYEDAQFGCLAWSGSESRVLYVAEKRHRKAESSCLSESGPANDREGAGPAAEEEEPTQKEDQCVYWEDWGEGLAGKSTPILCVADVDKGSVSVLRGVPRQVSPGQAFWAPGDGAVVFVGWWHEPFRLGLRFCTNRRSALFSLDLEGNCELLSPDTTAVSSPRLSPDGRYIAYLQGQVFGPHAQCLRLQQFDRETRKTALLLDLVKRPGKGEFAGLYEPLLPRRCWAADSQRLVFSSARRSRKDVFVLDTVSRRVTRLSDSSEFGSWKLLAIERDLMVVSCSSINCPPCLKVGFLPPAGGEDRVSWVPLDDLQCVPSIEWQIMDFTPPPEEENKEYSGLDFDALVLKPRGSRAGARVPLVVFAHGGPHSQFSAEWNAFSAALVTVGFSVLMVNYRGSTGFGQDSIVSLIGNMGSQDVKDVQRAVVTLLQSDATLDPRRVAVLGGSHGGFLACHLVGQYPDFYRACAARNPVINYATLLGTSDIIDWRYTAAGLPYSFDQLPTADALAAMLEQSPIVHAPQIRAPVLLMLGGRDKRVSPHQGLELYRAMKSRGSPVRLLWFEEDGHSLSRVDTQSSCFVNVVLWFLRHLDIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NC48</accession>
    <name>W5NC48_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01015647">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015206853.1">
        <property type="nucleotide sequence ID" value="XM_015351367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NC48"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018207"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018239.1">
        <property type="protein sequence ID" value="ENSLOCP00000018207.1"/>
        <property type="gene ID" value="ENSLOCG00000014782.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684605"/>
    <dbReference type="eggNOG" id="KOG0587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183196"/>
    <dbReference type="HOGENOM" id="CLU_001831_2_0_1"/>
    <dbReference type="InParanoid" id="W5NC48"/>
    <dbReference type="OMA" id="QPSLQWP"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000014782">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="937"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="307"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="363"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="567"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="668"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="828"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="381"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="567"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="859"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1250" mass="142466" checksum="01726BA1225D718F" modified="2014-04-16" version="1">MAKDCTAKSLDKIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKMYSHHRNIATYYGAFIKKSPAGQDDQLWLVMEYCGAGSVTDLMKKTKGNCLKEDWIAYICREVLRGLSHLHFHRVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDKTIGRRNTFIGTPYWMAPEVIACDENPDSTYDYRSDLWSLGITALEMAEGAPPLCDMHPMRALFLIPRNPPPKLKSKKWTKKFSTFIESCLVKNYQHRPSTETLLRHSFVKDLSNERQIRIMLKDHLDRTRKKKGEKEETEYEYSGSEDEEEEMNEDEGEPSSIVNLPGESTLRREFLRLQQENKNRSEAQRQQQVQQQQLKDQEKYKKQLLAERQKRIEEQKEQRKRLEEQQRRQQREAGYLWPELQEMQWRDETDKDEKGFLEERLKFPEEHRRGEKKQVEDSGRQKIDGGGRPADFQRDKKKSEPLQLQNKKFHRTLPKDQTQLLLLQHDHKQKNKETQQVASSGPKSSANNQRKVIDERMKAADAHRSNLNRFAAGLPLHLQTRLGSGSSSSPCTPALQRASTQQNANLRLSRDTQHSSSMSDMAMSPLSPEQDDVFWDFSNSEELPPKVPERTTSKFYCRELSNHQKNSISGFQQLSLLDKVATPTSPLSTSSSTSSSQSGSSVFTEEHKKGFSKMDSPLPQNRHQQFKKSQDGCPGVPFQHQVAVHSSGIKSNPGPFVKATEYSSSSDEVGSSDDDERSHFRPVMRNGKGRFLRTTPEGIVLQPHCNLNQQQNVADLKSSHQQRALELCNQNYLLPDLLQQSENPEPQSRQRDSHTPQSPQNRVLSLDSPISSPPGKNSTSSSSSSFTPFIDPRLLQISPPQSPVGQSPSGCKAEPFRRENARKGSVVNVNPTNIRPQSDTPEIRKYKKKFNAEILCAALWGVNLLVGTENGLWLLDRSGQGKVYSLISRRRFQQMDVLEGLNVLITISGKKNKLRLYYLSWLRNKILRNDPDVEKRQGWTSVGDLEGCVHYKIVKYERIKFLVIALKYSVEVYAWAPKPYHKFMAFKSFSSLAYKPLSVDLTVEEGVRLKVIYGSVSGFHAIDVDSGTTYDLYLPTHIQGSIHPHAIIILPNSNGMEVLVCYEDEGVYIDTYGRIIKETVLQWGEMPASVAYLQSNQVMGWGDKAIELRSVETANLEGVFMHKKAQKLKFLCERNDKVFFASVQSGGSSQIYFMTLGQNSLFHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5ND53</accession>
    <name>W5ND53_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01025690">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025691">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01025692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015207491.1">
        <property type="nucleotide sequence ID" value="XM_015352005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ND53"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018562"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018594.1">
        <property type="protein sequence ID" value="ENSLOCP00000018562.1"/>
        <property type="gene ID" value="ENSLOCG00000015081.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684267"/>
    <dbReference type="KEGG" id="loc:102684267"/>
    <dbReference type="CTD" id="25830"/>
    <dbReference type="eggNOG" id="KOG1584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158662"/>
    <dbReference type="HOGENOM" id="CLU_027239_1_1_1"/>
    <dbReference type="InParanoid" id="W5ND53"/>
    <dbReference type="OMA" id="MVESCHQ"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015081">
        <property type="expression patterns" value="Expressed in brain and 8 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="46"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="284" mass="33133" checksum="43C6C6DD46E07653" modified="2014-04-16" version="1">MAESEAETPSTPIEFESKYFEYNGVRLPPFCRGKMEEIANFSLRNSDIWIVTYPKSGTSLLQEVVYLVSQGVDPDEIGLMNIDEQLPVLEYPQPGLDIIQELTSPRLIKSHLPYRFLPTAMHNGESKVIYMARNPKDLVVSYYQFHRSLRTMSYRGTFQEFCRRFMNDKLGYGSWFEHVQEFWEHRMDSNVLFLKYEDMYKDLATLVEQLARFLGVSCDKAQLESMVESCHQLIEQCCNPETLSICRGRVGLWKDIFTVSMNEKFDAVYKQKMGKSDLTFDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NDI4</accession>
    <name>W5NDI4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spi-C transcription factor (Spi-1/PU.1 related)</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01025484">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NDI4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000018693"/>
    <dbReference type="Ensembl" id="ENSLOCT00000018725.1">
        <property type="protein sequence ID" value="ENSLOCP00000018693.1"/>
        <property type="gene ID" value="ENSLOCG00000015193.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3805">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155067"/>
    <dbReference type="InParanoid" id="W5NDI4"/>
    <dbReference type="OMA" id="HQCEYDL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015193">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000736">
        <property type="entry name" value="transcription factor Spi-C-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPI-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="119"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="261" mass="30086" checksum="E803862DFC6B8AAC" modified="2014-04-16" version="1">NAVAEGYTVKQSYLDQDILSQHFQDAIDVLQRHSDGTQQYQSEYKFYDAQGSQPAPVRTSFGCYSSPLVPDAPVYNWSELPPWAHTVPSAALTHTPPPENQLAYSVFAQQRGGKGRKKLRLYEYLHEALYDTNMADSIQWVDKGSGTFQFVSKNKEKLAERWGKRKGNRKTMTYQKMARALRNYSRTGEIIKIRRKLTYQFNPVILQRLSPSHPSGRETLYFQYIPTEQGYCSMDTWPNCYSSFNCDNEYDLNCTPIQSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEB7</accession>
    <name>W5NEB7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein AEBP2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the AEBP2/jing C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01026443">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01026444">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEB7"/>
    <dbReference type="FunCoup" id="W5NEB7">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000018976"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019008.1">
        <property type="protein sequence ID" value="ENSLOCP00000018976.1"/>
        <property type="gene ID" value="ENSLOCG00000015421.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048519"/>
    <dbReference type="HOGENOM" id="CLU_029789_0_0_1"/>
    <dbReference type="InParanoid" id="W5NEB7"/>
    <dbReference type="OMA" id="CWDQCHT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015421">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000323">
        <property type="entry name" value="zinc finger protein AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000471">
        <property type="entry name" value="Zinc finger protein AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052130">
        <property type="entry name" value="AEBP2/jing_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059034">
        <property type="entry name" value="SH3_AEBP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26014">
        <property type="entry name" value="SH3_AEBP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="143"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000018976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="298" mass="33428" checksum="296BEDF187D76044" modified="2014-04-16" version="1">VRFSLQLVTRLSPLHSISSTLMDMESTVSSGRSTPAMMNGQGSTTPSSKSIAYTCCWDQCHTCFNSSPDLAEHIRSSHVDRQRGGVFVCLWKGCKVYKTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPTHFSQQNSSKVSSQPKVKEESPSKAGMNKRRKLKNKRRRSLPRPHDFFDAQTMDAIRHRAICLNLATHIESLGNGHSVVFHSTVIARRKEDSGKVKVLLHWTPEDILPDVWVNENDRPQLKTKVVHLSKLPQDTAVLLDPNIYRSLLQYLLFLVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NEJ7</accession>
    <name>W5NEJ7_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carnitine/acylcarnitine carrier protein-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01031363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NEJ7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019056"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019088.1">
        <property type="protein sequence ID" value="ENSLOCP00000019056.1"/>
        <property type="gene ID" value="ENSLOCG00000015482.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167069"/>
    <dbReference type="HOGENOM" id="CLU_015166_16_0_1"/>
    <dbReference type="InParanoid" id="W5NEJ7"/>
    <dbReference type="OMA" id="GVGGMCN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015482">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000051">
        <property type="entry name" value="Mitochondrial carnitine/acylcarnitine carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF23">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE_ACYLCARNITINE CARRIER PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="109"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="208"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="301" mass="32611" checksum="D1DF68B5E63F9EA0" modified="2014-04-16" version="1">DLMGEIQQVSPVKNFFAGGVGGACLLLAGHPLDTIKVRLQTQPKVACGQHVIYTGTFDCFKRTVAREGMLGLYKGMAAPLAGVTPMMAIGFFGFGLGKRLQQQDPNTPLTYPQIYAAGMLAALCTTGIVAPGERIKCLLQVQSSSGVLKYSGPVDCAVQLYKEQGIRSIYKGTVLTLMRDVPSSGVYFMSYEWLKNVLTPEGQSVNDLSTPRILFAGGMAGILNWAVAIPVDVLKSIFQTAPEGRYSGFVDVLRELLREEGLRSLYKGFSAVMLRAFPANAACFLGFEVSLKFLNWLAPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFB6</accession>
    <name>W5NFB6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Double zinc ribbon and ankyrin repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01037501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFB6"/>
    <dbReference type="FunCoup" id="W5NFB6">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSLOCT00000019357.1">
        <property type="protein sequence ID" value="ENSLOCP00000019325.1"/>
        <property type="gene ID" value="ENSLOCG00000015699.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QTJR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000549"/>
    <dbReference type="HOGENOM" id="CLU_024089_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFB6"/>
    <dbReference type="OMA" id="GFAHIRS"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015699">
        <property type="expression patterns" value="Expressed in testis and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042462">
        <property type="term" value="P:eye photoreceptor cell development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052481">
        <property type="entry name" value="DZAN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026876">
        <property type="entry name" value="Fn3_assoc_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16058">
        <property type="entry name" value="DOUBLE ZINC RIBBON AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16058:SF4">
        <property type="entry name" value="DOUBLE ZINC RIBBON AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13287">
        <property type="entry name" value="Fn3_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DZANK-type" evidence="11">
        <location>
            <begin position="230"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="11">
        <location>
            <begin position="358"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="667"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="571"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="779" mass="84425" checksum="DF7AA9E908B1D096" modified="2014-04-16" version="1">MTAGSIVAPQIIPIRIPLPGKAKHQIDTNTPVEIKSDTPDVSIFFTLDGSKPEVLKKPGFGESSTLRYRGPVALPEGKVTVKALAVSRDGRESAVVTKFFLVEYLPPPDDPALLEDDKDNFLEEYTQESLEGSAFFPGGRRAAGSAWEETAQKLQAVKIQGRLSHKPPKGPHYLTTRLGPLWPGSEPVSALSSHRLQVQQAPSGTADNPRKSLTSTQMSRIQRETDFLRCAQCLSPRPSDPFARFCLQCGAPVPPVPGQRLPPPEGGQMVMCVHCKTMVPLNTSTCVICEAPLAPQLQAQAGIRLQDKLICQVCGTGNPVHISHCVTCETKLPESLNAVFGGQSATPVPISDGKMLSCSKCGRVNTSDARFCDWCGAKPAQPVSYVTCSRCGASSHPYANFCGSCGVYLEAPPRPDSRASMSWSALDPDQIRSMQAQDATWQPLSLSLPQPWAEQTQRVDRQTQTVGLFYPSSTELQKKEQQVAQQLEKQEQMRDRKPLLTAVSPGRGYWRKQLDHICAHLRSYTQNNTEFRALIGEPRMGKMISAVIQEDNYEVSLRINFVLAGDKHGLTGNPFRPSGNNLLSSVTEGRSSLSGSQASLGSENNVLFAAVPKKSKKKKRKPLETQDSVPSRDRQLLQELGPQGRGRISVVQQLLDEGADPNHLNSDGRPALTVAVLGGHHEAIPVLVHKGADIDQQSGPVNNTALHEVAALGNQGLQCAETLLGCNASIRKKNDKGLTVYDLAVKSGCNPLISLFAAKMGQGLVDKLSKPRTVSLDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFC0</accession>
    <name>W5NFC0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">DNA-directed RNA polymerase III subunit RPC6</fullName>
            <shortName evidence="8">RNA polymerase III subunit C6</shortName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination. Directly interacts with POLR3C. Interacts with TBP and TFIIIB90 and GTF3C4. Interacts with MAF1. As part of the RNA polymerase III complex, interacts with PKP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01037501">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006638554.1">
        <property type="nucleotide sequence ID" value="XM_006638491.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFC0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019329"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019361.1">
        <property type="protein sequence ID" value="ENSLOCP00000019329.1"/>
        <property type="gene ID" value="ENSLOCG00000015701.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102684479"/>
    <dbReference type="KEGG" id="loc:102684479"/>
    <dbReference type="CTD" id="10621"/>
    <dbReference type="eggNOG" id="KOG3233">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009679"/>
    <dbReference type="HOGENOM" id="CLU_033661_1_0_1"/>
    <dbReference type="InParanoid" id="W5NFC0"/>
    <dbReference type="OMA" id="VGTTKKC"/>
    <dbReference type="OrthoDB" id="613763at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015701">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000116">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000237">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028763">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="314" mass="35391" checksum="0ACF814B651E3FD8" modified="2014-04-16" version="1">MAEVKVKQESADPVDIENRIKELCQQFPHGITDQVIQNDMPHVEPQQRAMAINRLLSMGQLDLLRNSNGLLYRLKDAQVASKMKGSDNQEKLVYQVIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAEAARDSKQSPMVQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGQMKLYRGVNPIIQPTGLVKTPCGVCPVFDDCHPGGEISPSNCIYMTEWLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFJ7</accession>
    <name>W5NFJ7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-dependent metalloprotease SPRTN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Protein with SprT-like domain at the N terminus</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Spartan family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008283">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFJ7"/>
    <dbReference type="FunCoup" id="W5NFJ7">
        <property type="interactions" value="530"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019406"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019438.1">
        <property type="protein sequence ID" value="ENSLOCP00000019406.1"/>
        <property type="gene ID" value="ENSLOCG00000015764.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003585"/>
    <dbReference type="HOGENOM" id="CLU_019426_3_1_1"/>
    <dbReference type="InParanoid" id="W5NFJ7"/>
    <dbReference type="OMA" id="PSSANCM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015764">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000331">
        <property type="entry name" value="E3 ubiquitin-protein ligase RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044245">
        <property type="entry name" value="Spartan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055220">
        <property type="entry name" value="SPRTN_ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220:SF0">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22934">
        <property type="entry name" value="SPRTN_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBZ4-type" evidence="18">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="295"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="425"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="603" mass="66635" checksum="C8E75969BA369843" modified="2014-04-16" version="1">MDEDFLLALQLQDQFDQEASVSIVDDDDDYVQNKKRRVETSDELVTYPRSSFRPERPLSIVDESWEMLDPSPDVRAMFLQFNDMFFWGRLAGVEVKWSPRMTLCAGVCSYEGRGGLCSIRLSEPLLKLRPRRDLVETLLHEMIHALLFVTQNNRDRDGHGPEFCKHMNRINKASGTNITIYHSFHDEVDLYRTHWWRCDGPCQTRRPFFGYVKRATNRAPSARDPWWPQHQQTCGGTFIKVKEPEDYGKKGKKAENNKKGGTQQKDKSTPTAAGHSGSLDIRTLIPFSGKGFVLGHSSQATSSQEVPRDGRPSSEHKSTPSLLGSPPGNRKAEDAGALEWPGLSSSLKNSSTGSGDPKSGGNQSVPPKGNPTGKRSVGNSKAFVNINGSPIRILKANGQLQKPLSNSRDFFGAKQRLVNDLFQAKGAKSLEGPGVSKGGTISSKAQSEVRGQSKLMKNERSPTDESHSKGFKASEKRGGVSATEVQKSDTSEIPGCSRDVKPSPFEPLHSKYFKSPGKSKTDVPNARKRLRDEGSSAHIFDFFQRTTTEWSKPQTLAESTVTTTPSPVVNIPPSSANCMVSCPVCHTKVPESTINEHLDTCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFL1</accession>
    <name>W5NFL1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative ATP-dependent RNA helicase DHX57</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAH box protein 57</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Probable ATP-binding RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="AHAT01034932">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034933">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="W5NFL1">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019420"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019452.1">
        <property type="protein sequence ID" value="ENSLOCP00000019420.1"/>
        <property type="gene ID" value="ENSLOCG00000015774.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156883"/>
    <dbReference type="HOGENOM" id="CLU_001832_4_1_1"/>
    <dbReference type="InParanoid" id="W5NFL1"/>
    <dbReference type="OMA" id="PERVYVQ"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015774">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17985">
        <property type="entry name" value="DEXHc_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23825">
        <property type="entry name" value="RWD_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000325">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF145">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1176"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="205"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="596"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="872"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="468"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="790"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="1431" mass="158408" checksum="0C03990A75FAC73E" modified="2014-04-16" version="1">AMSGRRKGKPNRGGGRFNKSGGGGARGGGGTRKGADSAWDDGDDFVLFSGPSRPARGRAGGGRGGGGGRGGGSGRGGGSGRGGSSGRGGGGASGSSFGRGRANRRSVPDQPRPRPEVGSRPRAKPDLPQMPMQTIHMTSENQARVKELLRELQSQDFEEPDVDSETEEEEEEEYDDLDYREEGQFWSTTDDPVGSSQEISLFEPDSEEELQVAEAPVSLFAIGRLCRYGFDRGRSRLTLEACGGEVGASLELLLGQVTTEKYGEGARGAKGAGGVSAEDCLAQRREEALALASIFGERFTERIRDRVWTVALEPYFLAWAGGDCPDQTSAKASGPVLLKDVCRFYLKGAGCRFGHKCRFKHQLPTEPGGAKKPVGPSQPGIDSYSPPEYELEIRFPAGHLYPFQAPVVAFSTNDEKVSPAGRLHVSEYLFGEALVAAESGEPVVYTLVTCCEDEAKMKELLANSHHKYSAPSPVVSAAPPAKSRSARSSAPSLDLLANSISKHLEMKDPDPEKEHVELELEEEGEEEETPVVPVENDSYVNFRKLARRHEWKPESTQQENAKLCRQFRSKQNSRRFLSMLQQRQKLPAWQERENILHLLNECQVLVVSGMTGCGKTTQIPQFILDASLKGPAGKVANIICTQPRRISAISVAERVAQERAERLGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGEADLRGVTHVIVDEVHERTEESDFLLLVLKDLMIQRPDLKIILMSATLNAELFSQYFYNCPTINIPGRTFPVDQFFLEDAIAKTRFVLEDGSPYARSGKQAGQPGRGSSRRQPVEQPEEDTWSFTHFQKPDFVKDSIPDQQLTLQQLSVRFKEDVQKSVLKTIVSMDLDKINMDLIESILEWIVDGKHNYPPGAVLVFLPGLAEIKMLYEQLQSNRLFNNRNKKRCVVYPLHSSLSNEEQQAVFNRPQEGVTKIIISTNIAETSVTIDDIVYVVDSGKMKEKRYDASKSMESLEDSWVSRANALQRKGRAGRVASGVCFHLFTSHCFHHQLAEQQLPEIQRVPLEQLCLRIKILDLFAERMLESVFSQLIEPPAAGSLEAAKQRLQDLGALTLDEKLTPLGYHLACLPVDVRIGKLMLFGAIFRCLDPALTIAASLAFKSPFVSPWDKREEANVKKLEFALANSDHLALLQAYKGCRTVLHSLICLGFCLCLLVLVSLWSLSLQEIASLKRQFAELLSDIGFVKEGLNARVIERMCSKGVDGILEATGFEANLNSDNIKLMSAMLCAALYPNVVQVRAPQGKYKLTSKGAMKMQPKADELRFMTKNDGCVHVHPSSVNYQVRHYDSPYLVYHEKVKTSRVFIRDCSMVSVYPLVLFGGGQVNVELQRGEFVISLDDGWIRFAAASHMVAELVKELRWELDQLLEDKIKNPSMDLCSCPRGSRIIAMIVKLISTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFL3</accession>
    <name>W5NFL3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Death-associated protein kinase 1-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AHAT01016212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015207751.1">
        <property type="nucleotide sequence ID" value="XM_015352265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFL3"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019422"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019454.1">
        <property type="protein sequence ID" value="ENSLOCP00000019422.1"/>
        <property type="gene ID" value="ENSLOCG00000015776.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107077995"/>
    <dbReference type="KEGG" id="loc:107077995"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169289"/>
    <dbReference type="HOGENOM" id="CLU_309928_0_0_1"/>
    <dbReference type="InParanoid" id="W5NFL3"/>
    <dbReference type="OMA" id="CKNITTE"/>
    <dbReference type="OrthoDB" id="9988411at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015776">
        <property type="expression patterns" value="Expressed in bone element and 9 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47679:SF2">
        <property type="entry name" value="C-TERMINAL OF ROC (COR) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47679">
        <property type="entry name" value="PROTEIN TORNADO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="35"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Roc" evidence="5">
        <location>
            <begin position="281"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="950" mass="106966" checksum="D152A66441904A74" modified="2014-04-16" version="1">MSEEEFLRVVKTHPLQTIQDYLTQQGVNINYCDEFSNTALHYAAHNRSREVARLLVCSGGNILHENMDGLTPIHFAAFGGNIEVLTELLLKVPEAVNLQSHKNLSTPLHEAVSGKIVQAVECLLSKGADCNSQNQNGDTPLHLAVSQNSEHICCLLLRHGANVLLRNKRGDTCLHSAAARGALSICQMLVRNGASMQAKNKNSETALFSAVRGTGNKNGDDYCELLSWMVEELGPCLLYRNKDGLRVLDVARQSNLPSKSEVLLQQKTQELQIRDLLKESKPIDRRVAKLCICGHSGVGKTTLAKTLQQQQDGFLSKFQYWFSRPDPPSSTQGVAITEGNLSAGKVVIWDFAGQMEYYFTHSLLLSTESGNVLYCIVFSLENIQCDKGGTQSEALKQVLYWLRFLNVCRNSGVAHKAKVFLVGSHKDTLEIECKDEVVNHFFDIVQSRVRELGTNLDIDTTVLSVNCKSPSDLQTLRKRLEECVAQLQQSTTDTKFPGICCEVLDEIHTIRCNGSVKYLLWGDFFASINKNRFEKISVETLRTAVEYLHNVSELLFFPTVTVDGPTQSAESGTGNGLVILDVQWLCQDIFGKFGHFALSTLPLQREKWTTENIASLLNLEDSPSTVLRLLEILELVFSEKEGQYIVPAWLNKGRPANIWEREEVFNVYHGVSFGWQSELGLFSQAFFCRLQLRLMKLFTQTTEDGASAEKFIIWTDGMKCIDTAEALVQVSAGRGSINIAVRGYKPQRSGSRYPDTRRKCYELQNVISCQVQHLLQEFKVGSECEMLFLSSKDLLDKMDASDVDITAYTKDEILLSARSGRNLYSKLKGREEYLHDVLLAGYDQTILQSLKWEATIQWMTAECTEKLCALLDPVHPLGNYWKRLMEALEDCTYENVEALATQSEQAWVSPTLLLFQKHCHTTIYSLYKAFEKLEREDCMSHVEIMLNNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NFS2</accession>
    <name>W5NFS2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Endoplasmic reticulum oxidoreductase 1 beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034954">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NFS2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000019481"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019513.1">
        <property type="protein sequence ID" value="ENSLOCP00000019481.1"/>
        <property type="gene ID" value="ENSLOCG00000015824.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007753"/>
    <dbReference type="HOGENOM" id="CLU_023061_2_2_1"/>
    <dbReference type="InParanoid" id="W5NFS2"/>
    <dbReference type="OMA" id="NENCFTR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG16"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015824">
        <property type="expression patterns" value="Expressed in liver and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF2">
        <property type="entry name" value="ERO1-LIKE PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="16">
        <location>
            <begin position="108"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="474" mass="54463" checksum="16AEB3A834DCB759" modified="2014-04-16" version="1">MLKITTQIGGISFFLWLLVGDLVLGWLNERQRRESAGRVSPFDNLDQSCFCHLTGVLDDCFCDIESIDVFNNFKIYPRIQKLTERDYFRYYRVNLKRPCPFWPDDSHCAIKDCQVEPCPESKVPVGIKAGNYNKYSQAANAIKEIEDCEQAHELGAINSTLRLQSCFFSAAAEHIGADRSCSSDETSPDAEYVDLRLNPERYTGYKGPSAWRVWNSIYEENCFKPRSVYRPLNPLAPSKGDDDGEGFYNWLEGLCLEKRVFYRLISGLHSSINIHLCAKHLLDEGWGKTVWGPNVKEFHHRFDPAETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVNLYTGNAQEDTETKDLLLQILNETKAFPMHFDEKSMFAGDKMEAKTLKVSLFSPRHREMKSSHGAVRKGCQVPGSSEVIIIIINENLKNRMKENVKVFLLPSVFGIFFLCSLSRLSTSIKELHNFRMLLKDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5NGE5</accession>
    <name>W5NGE5_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrator complex subunit 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008442">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006625762.1">
        <property type="nucleotide sequence ID" value="XM_006625699.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NGE5"/>
    <dbReference type="FunCoup" id="W5NGE5">
        <property type="interactions" value="1752"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000019704"/>
    <dbReference type="Ensembl" id="ENSLOCT00000019736.1">
        <property type="protein sequence ID" value="ENSLOCP00000019704.1"/>
        <property type="gene ID" value="ENSLOCG00000015999.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102686109"/>
    <dbReference type="KEGG" id="loc:102686109"/>
    <dbReference type="CTD" id="55756"/>
    <dbReference type="eggNOG" id="KOG1138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001445"/>
    <dbReference type="HOGENOM" id="CLU_023159_1_0_1"/>
    <dbReference type="InParanoid" id="W5NGE5"/>
    <dbReference type="OMA" id="AMKAVHC"/>
    <dbReference type="OrthoDB" id="5600060at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000015999">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090101">
        <property type="term" value="P:negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090287">
        <property type="term" value="P:regulation of cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16294">
        <property type="entry name" value="Int9-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000003">
        <property type="entry name" value="Integrator complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027074">
        <property type="entry name" value="Integrator_9su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048660">
        <property type="entry name" value="IntS9-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21382">
        <property type="entry name" value="IntS9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-Casp" evidence="8">
        <location>
            <begin position="305"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="554"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000019704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="658" mass="74024" checksum="1855EF4B5EB103FA" modified="2014-04-16" version="1">MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSALHFLPLPLVHSQRLSRLPGWVSKDGNTLLEKELKECAGRVFVDSLPEFCLPEKELLDLSTIDVILISNYHCMMALPYITEHTGFTGMVYATEPTFQIGRLLMEELVTFMERVPKAHCATCWKNKEIQRMLPAPLKDALEVWTWKRCYSMQEVNSALSKVQLVGYSQKIELFGAVQITPLSSGYALGSSNWIIQSHYEKVSYVSGSSLLTTHPQPMDQSSLKNSDVLILTGLTQIPTANPDGMLGEFCSNLAMTIRAGGNVLVPCYSSGVIYDLLECLYQFIDSANLSSTPFYFISPVANSSLEFSQIFAEWLCHNKQSKVYLPEPPFPHAELIQTNKLKHYPSIHGDFSNDFRQPCVVFTGHPSLRFGDVVHFMELWGKSSLNTIIFTEPDFSYLDALAPYQPLAMKCVYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPTQSHRSDLMLDCQPPPMPYHRCSVLSLPFRRRYERIELLPELAKSLVPSEIKSGISVATVSAVLQSKDNKHVLQPPPKPAPAPPSKKRKRVVEEAPELRAPKPLLSGSVPLEQFLATLQKHGITEVKVEDTPDGHILHLQDEDTLIQLEEDSTHIVCDNNESLRATLRDLVLRFLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5NIW7</accession>
    <name>W5NIW7_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hippocalcin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01027755">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_006626106.1">
        <property type="nucleotide sequence ID" value="XM_006626043.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NIW7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000020576"/>
    <dbReference type="Ensembl" id="ENSLOCT00000020611.1">
        <property type="protein sequence ID" value="ENSLOCP00000020576.1"/>
        <property type="gene ID" value="ENSLOCG00000016657.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102688017"/>
    <dbReference type="KEGG" id="loc:102688017"/>
    <dbReference type="eggNOG" id="KOG0044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154645"/>
    <dbReference type="HOGENOM" id="CLU_072366_1_0_1"/>
    <dbReference type="InParanoid" id="W5NIW7"/>
    <dbReference type="OMA" id="RQHTEFN"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG1"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016657">
        <property type="expression patterns" value="Expressed in heart and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000078">
        <property type="entry name" value="Hippocalcin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000072">
        <property type="entry name" value="Hippocalcin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF79">
        <property type="entry name" value="HIPPOCALCIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000020576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="193" mass="22255" checksum="F1DCE0FC0DDE35D0" modified="2014-04-16" version="1">MGKQNSKLRPEVLNDLRENTEFTDHELQEWYKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNNDGKLSLEEFIKGAKSDPSIVRLLQCDPSSASQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5NK54</accession>
    <name>W5NK54_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLC6A13</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034335">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NK54"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021013"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021049.1">
        <property type="protein sequence ID" value="ENSLOCP00000021013.1"/>
        <property type="gene ID" value="ENSLOCG00000016994.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157478"/>
    <dbReference type="HOGENOM" id="CLU_006855_9_5_1"/>
    <dbReference type="InParanoid" id="W5NK54"/>
    <dbReference type="OMA" id="ANYTSHA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG8"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000016994">
        <property type="expression patterns" value="Expressed in muscle tissue and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF111">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="623" mass="70795" checksum="412AB0C355F2A4E7" modified="2025-10-08" version="2">MFGRCGGDMENRFRVDASNGHSRPLEIVAQKEEKIKDRGQWNNKVEFVLSVAGEIIGLGNVWRFPYLCYRNGGGAFFIPYLIFLFTCGIPVFFLETSLGQYTSEGGITCWRKISPLFEGIGYATQVIVALLNFYYIVVLAWGIFYLCNSFTWDLPWSSCNNTWNTETCVEFQRRNESFNSTVAANATSPVIEYWENRVLRISSGIDHIGVLHWDLSLCLLAAWIICYFCIWKGVKSTGKVVYFTATFPYFMLIILLIRGVTLPGAAEGIKFYLYPDLGRLADPQVWMDAGTQIFFSYAICLGCLTALGSYNKYSNNCYKDCLALCFLNSGTSFVAGFAIFSILGFMSYEQQVPISEVAESGPGLAFIAYPRAVSMMPFSPVWACFFFIMIVLLGLDSQFVCVESLVTAMVDMYPSVFRRRHRRELFILAVAVVSFIMGLIMLTEGGMYVFQLFDYYAASGMCLLFVAIFETVCIAWVYGADRFYDNIEDMIGYRPSSVIKYCWLFFTPAVCFGTFAFSLIKYTPLKYNNVYVYPWWGYGLGWLLALSSMVCIPLWIIFKIITTKGTFRQRIQQLIRPSDDLPKTKKEQEKLLAIFAPETGLQQPLGEPTREGYFPVPEKESVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5NKU2</accession>
    <name>W5NKU2_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysosomal-associated transmembrane protein 4A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LAPTM4/LAPTM5 transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01029896">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_006626411.1">
        <property type="nucleotide sequence ID" value="XM_006626348.3"/>
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        <property type="interactions" value="1009"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000021251"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021287.1">
        <property type="protein sequence ID" value="ENSLOCP00000021251.1"/>
        <property type="gene ID" value="ENSLOCG00000017194.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102698671"/>
    <dbReference type="KEGG" id="loc:102698671"/>
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    <dbReference type="eggNOG" id="ENOG502QSAX">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="entry name" value="LAPTM4/5"/>
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        <property type="entry name" value="mtp"/>
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        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
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        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN 4A"/>
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        <property type="entry name" value="Mtp"/>
        <property type="match status" value="2"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="238" mass="27332" checksum="00E307F537888E8F" modified="2014-04-16" version="1">MFLPKMEDNVNFKPSRDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMGILLTVAVTHPDNVPTVDLQYEVIEHYFASDRMAENACVGFAISLLMFTISAMMVYGAVTHRDGWLIPFFCYQLFDFALSCLVAISSLTYLPRIKDYLDQLPDFPYKDNLLSLDSSCLLLIVLIFFAFLIILKAYLINCVWNCYKYINNRNLPEIAVYPAFEAPPQYVLPTYEMAVKMPEKEPPPPYMPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5NLH2</accession>
    <name>W5NLH2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lysophosphatidic acid receptor 6-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01022483">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015360700.2"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSLOCT00000021518.1">
        <property type="protein sequence ID" value="ENSLOCP00000021481.1"/>
        <property type="gene ID" value="ENSLOCG00000017376.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_009579_8_2_1"/>
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        <property type="component" value="Linkage group LG14"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in bone element and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
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        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="346" mass="39385" checksum="67EC3D79A03F1DBB" modified="2014-04-16" version="1">MEYKGNETSAENTSFLPNVNMIKCTFRDKNKYTVYSVVYGMVFAIGLVTNTAALYIFCRLAKKKRVSTAFLMNLAIADLIFILTLPFRIFSYQTETSWPFLDVACRVSTYLFYMSMYCSIFFLASLGVCRYLVMAGRVKFQNELLYRWALALCFAVWIFIAAGVIIYASFSNGFRIEAGKCFEPAEPSSWELLYYMNFYALVIGFVIPFVILLICYALMIKHIAQTKTQKRRRDVAMICLVLTVFCICFLPYHVQRTLHLYYVVYHHDNCELLITLQKTVVATLCFAVANSCMDPLLFVFVGQGFLPMLKTLLRSCCLGFSNGTPISTSAATDNPENVITVATTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMB8</accession>
    <name>W5NMB8_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Immunity-related GTPase family, q2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01019544">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMB8"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021777"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021814.1">
        <property type="protein sequence ID" value="ENSLOCP00000021777.1"/>
        <property type="gene ID" value="ENSLOCG00000017672.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102687255"/>
    <dbReference type="KEGG" id="loc:102687255"/>
    <dbReference type="CTD" id="556650"/>
    <dbReference type="eggNOG" id="ENOG502RPY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165579"/>
    <dbReference type="HOGENOM" id="CLU_015342_2_0_1"/>
    <dbReference type="InParanoid" id="W5NMB8"/>
    <dbReference type="OMA" id="QGVKQPK"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG24"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017672">
        <property type="expression patterns" value="Expressed in camera-type eye"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="37"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="377" mass="41926" checksum="EEF186F7F6A5A3BA" modified="2014-04-16" version="1">MMAEVLKNSKVLQTIKDSLDKNTMSDIKATLEDLLSNCLNIAVIGKRVPEKMTFINSLRGLGDDDVGAAHAGPADTNKEPIMYPHPQHSDVRIWDLSMPESNECNPNEYLETVKVNHYCSLIFTVSERLAVAYAALWKELHKGEKELYFILLTSVTDSSDSLLEKKRSIMESFKNQGVKSPQVFLVSSSALHQLEFMDLLEAMEKNIPELRMNALLFSLPTFSVPILKKKRAAFKAVVWTAASLSGGASTIPLPTVSSVLDVNMLMKILNKARDSFGLDNKSLERLAKQARLPVAELESARTSELSIEISKLVIQRKLALAEKERSLGAKLVDTALTGSVKSMTKSFTTMYFMLTASLDELEEDAVRVLRKAYGDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMG4</accession>
    <name>W5NMG4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01031870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NMG4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021823"/>
    <dbReference type="Ensembl" id="ENSLOCT00000021860.1">
        <property type="protein sequence ID" value="ENSLOCP00000021823.1"/>
        <property type="gene ID" value="ENSLOCG00000017718.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287968"/>
    <dbReference type="HOGENOM" id="CLU_077975_9_3_1"/>
    <dbReference type="InParanoid" id="W5NMG4"/>
    <dbReference type="OMA" id="YSTGQDN"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG7"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017718">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF28">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867944" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13356" checksum="C18FB832F83F488B" modified="2014-04-16" version="1">KTSMLILLLYFIFSDLTFAYDVIQTPEKVLEHPGKTVKLQCEYDGTTTNYIYWYRQVGEGGLSLIGYSVGVGIINEEAEFKASRFVMNRNETKKSTLKIQPLESADTAVYFCASSMTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5NMV9</accession>
    <name>W5NMV9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 78 member Ab</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5NMV9"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000021968"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022007.1">
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        <property type="gene ID" value="ENSLOCG00000017865.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QPXK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018059"/>
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    <dbReference type="InParanoid" id="W5NMV9"/>
    <dbReference type="OMA" id="VSMDDNF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000017865">
        <property type="expression patterns" value="Expressed in intestine and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029638">
        <property type="entry name" value="FAM78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655:SF3">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000021968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="180" mass="20621" checksum="97AFC9E932CB7327" modified="2014-04-16" version="1">IAVFCFRSSWEIPDLRDGKIQAISDSDGVNYPWYGNTTETCTIVGPTKKNTEIKVSMDDNFDTSVSWNVPKRNSNLHQLTSIHRDQSFITWLVAINQSTREHLILKTIMWRMQLHIKIDPRKPLGQRAKLLKPTTQEQPQILAKKEPIPPNAMVKPNANDTQVLMWRPKKGKPVVVTPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNB4</accession>
    <name>W5NNB4_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01015391">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5NNB4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000022123"/>
    <dbReference type="Ensembl" id="ENSLOCT00000022164.1">
        <property type="protein sequence ID" value="ENSLOCP00000022123.1"/>
        <property type="gene ID" value="ENSLOCG00000018022.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S5N3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154669"/>
    <dbReference type="HOGENOM" id="CLU_133580_0_0_1"/>
    <dbReference type="InParanoid" id="W5NNB4"/>
    <dbReference type="OMA" id="PRPRIFY"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG4"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000018022">
        <property type="expression patterns" value="Expressed in mesonephros and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="129" mass="15082" checksum="9CC5A5D0B52CF067" modified="2014-04-16" version="1">SHNITKEETVALKHLKENKHIVIKPADKQYIAEAQRQLNNQEHYTTLEEPLYKQTALEIGQILQTLVELKHLTKKQAFYLRGEESPRPRIFYLLPKIHKAPETWPLPYEIPPGRPIVSDCNSESYRIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5NNQ6</accession>
    <name>W5NNQ6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">X-C motif chemokine receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Linkage group LG9"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="CELL ADHESION MOLECULE"/>
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    </dbReference>
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        <property type="entry name" value="CHEMOKINE XC RECEPTOR 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
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    </dbReference>
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        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="179"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="210"/>
            <end position="231"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="287"/>
            <end position="310"/>
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    </feature>
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        <location>
            <begin position="60"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000022265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="353" mass="40707" checksum="01248D1DA0BF0CF6" modified="2014-04-16" version="1">MDTSSEQLSTTAGYVENQFSTYEYNDYYEDDVCNKETVSRFGDIITSVFFTLVIVLSVLGNLLVLGILAKYENLKSVTNIFILNLALSDLIFTFGLPFWASYHSEGWAFGESVCKAVNFIFDVGFYSSTIFLTMMTIHRYLAVVHPLADLGSRKCCYGITASVVIWLLSCLAAVPGLLFHRVKLNQNENVPYCEYDSPLWKLAEIYQKNIFFLVAFSIIIFCYFQILKTLLRSRTHTRYRTVRLIFAIVVVFFVGWAPYNVIIFVRSLVYNQIHPFTDCEISRGIDYGFYICRMIAFSHCCLNPFFYAFVGVKFRNHLKKLLRKVWMCQASEDLQLGNSRVMFHSHGEDASIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5LVK4</accession>
    <name>W5LVK4_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Macroglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Semionotiformes</taxon>
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            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LVK4"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000161"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000161.1">
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        <property type="gene ID" value="ENSLOCG00000000147.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="W5LVK4"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000147">
        <property type="expression patterns" value="Expressed in pharyngeal gill"/>
    </dbReference>
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        <property type="entry name" value="VWA7_4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF18">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING 10, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="VWA7_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWA7 beta-sandwich" evidence="1">
        <location>
            <begin position="27"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="VWA7 Ig-like" evidence="2">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="19354" checksum="D892E2707EA47AAC" modified="2014-04-16" version="1">QTGLWEINVTSSRPCTIKVTDQSVIGFLYSFVQLFQGPHPGLALIEGRLQVGKNATLLVSVTGSESLRVSEVSLVSASGSRVQRGSITPLDRNDRREYLVSVPSVPAGQFSVLLIGVDNTSSTHFQRQSNTQFTSTSIEVKAQANTTLEPGKDFIIPFTVATNGMGETYTINARDDKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5LW99</accession>
    <name>W5LW99_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LW99"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000406.1">
        <property type="protein sequence ID" value="ENSLOCP00000000406.1"/>
        <property type="gene ID" value="ENSLOCG00000000373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_3_1"/>
    <dbReference type="InParanoid" id="W5LW99"/>
    <dbReference type="OMA" id="CHREPLC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003758">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="135" mass="14727" checksum="8084A4D2F7F3D894" modified="2014-04-16" version="1">GVSPRRQDLTSESTSLSSMDSSQVTVTQTPAVKSIAPEQTITVTCKTSRAVYNKNHLPWYQQTAGEAPKLLIYYATTHQTGIPEHFSGSGSGIDLTLTITGIQVEDAGDYYCQSFHLPNSIQTHRASVTTFCDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWD0</accession>
    <name>W5LWD0_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LWD0"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000437"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000437.1">
        <property type="protein sequence ID" value="ENSLOCP00000000437.1"/>
        <property type="gene ID" value="ENSLOCG00000000399.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502SR2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154869"/>
    <dbReference type="HOGENOM" id="CLU_077975_1_0_1"/>
    <dbReference type="InParanoid" id="W5LWD0"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000399">
        <property type="expression patterns" value="Expressed in intestine and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="216" mass="23287" checksum="88F7866FE3717A28" modified="2014-04-16" version="1">QIVLTQSPAQSILPGSSVSISCTASSSVISGRYSLLNWYLHKPGQPPQLLVYYATTHQSGVPDRFIGSGSGTAFTLTITGVQSNAGDYYCVQTLFSPFLHIQGCFNANLTPFKDKIFSRVVDDSKSSGQIVMTQTPPALSVLSGGNVTIHCKASSSITGYYNYLNWYQQKPGEAPKLLIYWATERHSGVPERFTGRGSGLDYSLTISGLQAEDAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5LWE1</accession>
    <name>W5LWE1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="W5LWE1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000448"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000448.1">
        <property type="protein sequence ID" value="ENSLOCP00000000448.1"/>
        <property type="gene ID" value="ENSLOCG00000000410.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160434"/>
    <dbReference type="HOGENOM" id="CLU_055524_12_0_1"/>
    <dbReference type="InParanoid" id="W5LWE1"/>
    <dbReference type="OMA" id="KADTYCT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000410">
        <property type="expression patterns" value="Expressed in brain and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03165">
        <property type="entry name" value="NET-5_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000006">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF40">
        <property type="entry name" value="TETRASPANIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="153" mass="17527" checksum="3F0729AA3BEA196A" modified="2014-04-16" version="1">MSSLYASLSRQFFVLLLLIFLLEALSIILFFAYKDEIDRYAQADLKRGLQFFGTEGNIGLTNAWSIVQTDFRCCGVSNHTDWFSVYNDTRVPDSCCLEYSENCGLERPDTWWTAVRPGVQVCLQPCYKQVKSWMQHNLLSLWVSALCTGVTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5LX12</accession>
    <name>W5LX12_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LX12"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000672.1">
        <property type="protein sequence ID" value="ENSLOCP00000000669.1"/>
        <property type="gene ID" value="ENSLOCG00000000610.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3Q7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004989"/>
    <dbReference type="HOGENOM" id="CLU_137596_2_0_1"/>
    <dbReference type="InParanoid" id="W5LX12"/>
    <dbReference type="OMA" id="HEIWNSA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000610">
        <property type="expression patterns" value="Expressed in liver"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="1">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="118" mass="13333" checksum="F2496BDEF2FB98FE" modified="2014-04-16" version="1">QYATWCVSDEELQKGGYLISENGNYKAVFQDDGNFVIYGWKSIWDSDTAETCNVDHLIMQSDSNLVAYKTDGKAMWQTNNHTSSGFEDCVLQLRNDGTLVIERDGEVWSSATSRGLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5LXL6</accession>
    <name>W5LXL6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Seven cysteines N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01009291">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LXL6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000000873"/>
    <dbReference type="Ensembl" id="ENSLOCT00000000877.1">
        <property type="protein sequence ID" value="ENSLOCP00000000873.1"/>
        <property type="gene ID" value="ENSLOCG00000000785.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QR8M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157613"/>
    <dbReference type="HOGENOM" id="CLU_738802_0_0_1"/>
    <dbReference type="InParanoid" id="W5LXL6"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG6"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000785">
        <property type="expression patterns" value="Expressed in ovary and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000257">
        <property type="entry name" value="Dyslexia-associated protein KIAA0319-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="KIAA0319-like"/>
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        <property type="entry name" value="MANSC_dom"/>
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    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182:SF3">
        <property type="entry name" value="DYSLEXIA-ASSOCIATED PROTEIN KIAA0319-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182">
        <property type="entry name" value="FI19480P1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23597">
        <property type="entry name" value="KIAA0319_N"/>
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    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <feature type="domain" description="Seven cysteines N-terminal" evidence="6">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
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        <location>
            <begin position="173"/>
            <end position="229"/>
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    </feature>
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        <location>
            <begin position="110"/>
            <end position="141"/>
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    </feature>
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        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="184"/>
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        <location>
            <begin position="192"/>
            <end position="201"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="202"/>
            <end position="229"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000000873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="375" mass="39922" checksum="ED1DA61EB50C5FF5" modified="2014-04-16" version="1">MLTPPPALALSTPLPGCSEAEACGVPGAAKAVPWPGVRGAGWRPLSTGQTWGAVQCVRACCVRPDCSAAWSQGGRCVLLSCPAPGRCHLSPTRLATPLARGQLWQLGQQDRGERRMKRSEEGDVGKMEKAQPYVNREDPTSDTHAAPDPPASGPAPPVTVANPLTLSLTVNRKGSLSTSGSTELSDPKLAAQNPTPSTGMVTTVTSNSSWSSTRTLEPTTPTSHTPSAPTQAVRELVVSAGNSVEITLPRSEVQLNAFVLPEPPKGTEYVFDWRLITHPHDYSGEMEGKHSRILKLSKLTLGLYEFKVLVDGEGAHGEGYVNVTVKPEPRVNKPPVAVVSPRFQEISLPTSSTVIDGSREYRGPGVWCPVSSAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYB7</accession>
    <name>W5LYB7_LEPOC</name>
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        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="W5LYB7"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001124"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001128.1">
        <property type="protein sequence ID" value="ENSLOCP00000001124.1"/>
        <property type="gene ID" value="ENSLOCG00000001000.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502S3KF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="HOGENOM" id="CLU_077975_4_1_1"/>
    <dbReference type="InParanoid" id="W5LYB7"/>
    <dbReference type="OMA" id="YHYINSH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001000">
        <property type="expression patterns" value="Expressed in bone element and 6 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="IgV_Light_Chain"/>
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    <dbReference type="PANTHER" id="PTHR23267">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865303" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="123" mass="13074" checksum="3AC1A82C97B6D9FA" modified="2014-04-16" version="1">TMMPLILMLGTLGLFAQESSGQIVLTQSPAQSVLSGSSVSISCTASSTITSGSTSYLHWYLRKPGQAPELLVYYATNRYSGVPDRFTGSGSGITFILTITGVQAEDTGDYYCGQSGYSFPFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYC1</accession>
    <name>W5LYC1_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Palmdelphin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the paralemmin family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01034668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYC1"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001128"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001132.1">
        <property type="protein sequence ID" value="ENSLOCP00000001128.1"/>
        <property type="gene ID" value="ENSLOCG00000000981.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QVMH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157718"/>
    <dbReference type="InParanoid" id="W5LYC1"/>
    <dbReference type="OMA" id="GIDNMTH"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000000981">
        <property type="expression patterns" value="Expressed in embryo and 7 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46881">
        <property type="entry name" value="PALMDELPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46881:SF1">
        <property type="entry name" value="PALMDELPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="547" mass="62046" checksum="F71CF1E7A23FFF83" modified="2014-04-16" version="1">MEEAELLKERLQAITDKRKIREDIAQKRTEIEEEKLKLQYLKKQKKALREKWLMDGLSSPSVPEEMALKLQAQDNQQQTKILESNISRIENEIEALERQEMMISTNESLILKKLKEVERSAEDIIKAVKADVKQAITEPIEYIYSEIPDLPKSYRPTNLKKMPSPEQQMDNEQSKKALFAMEINVEKDMKTGESQVLSTATVTPEAFQEKGIKVYDDGRKSVYALCSDREVAHNGVDELTPIEVEELLRKATEKKSKTALEYHEPVFSSSYSRPSTPRRRDRPQASPEPNGLQESPEKHTPMMPELAHNDMAHFQEEMASHIPHRPDGPPKFAETSPNGKTNQSIFSNGDETHLLSNIAKSEPMPYQDSRDAHHAAVFVNSVDDNKQSAAAYPDDPKCNVLHAMPTSVANEEPVTMIFMGYQCVEDDVETQQGMGFEGAIRAELVVIGDDDDNELQPSYHPDGYHSKVFQPSMNSRLSHYLPDTEVNCSTNNVLAYSDSVPLQDQETRPNHVPNRTYSDGQTSRDATEDISVTALRMRMTKLGKRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5LYX6</accession>
    <name>W5LYX6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AHAT01011260">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LYX6"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000001333"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001338.1">
        <property type="protein sequence ID" value="ENSLOCP00000001333.1"/>
        <property type="gene ID" value="ENSLOCG00000001172.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154587"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="W5LYX6"/>
    <dbReference type="OMA" id="NKMTEVT"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001172">
        <property type="expression patterns" value="Expressed in muscle tissue and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000119">
        <property type="entry name" value="Putative map kinase-interacting serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000093">
        <property type="entry name" value="Putative map kinase-interacting serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="87"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="475" mass="53368" checksum="A532A23AEDBDB0DE" modified="2014-04-16" version="1">MVQNKITEITGFHRSFKGQNPFESDDFTQNGSHLLDSAFNFDCPPRPDMPSSLPIDIPDAKKRNKKKKRCRATDSFSGRFEDPDVYRLQEEVLGEGAYARVQTCINLITNKEYAVKIIEKRPGHSRSRVFREVEMLYQCQGHRNILELVEFFEEEEKFYLVFEKLRGGSILTHIHKRRHFNEQEASIVVQDIASALDFLHNKGMAHRDLKPENILCEHEDRISPVKICDFDLGSGIKLNSDSSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYIMLSGYPPFVGRCGGDCGWDWGEACHSCQNMLFESIQEGKYEFPEKDWAHISSGAKDLISKLLVRDAKNRLSAAQVLQHPWVQGCAPNTLPTPILLQRNSSAKDLTFFAAEAVAVNRQLAQQEESETEEPRPVVVAAPSCSVRLSPPSQSKLARRRQKSSLLKGAPVSASELHQLLAPLVVVGDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5LZQ0</accession>
    <name>W5LZQ0_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CKLF-like MARVEL transmembrane domain containing 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01012323">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01012325">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5LZQ0"/>
    <dbReference type="FunCoup" id="W5LZQ0">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001607"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001612.1">
        <property type="protein sequence ID" value="ENSLOCP00000001607.1"/>
        <property type="gene ID" value="ENSLOCG00000001410.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048725"/>
    <dbReference type="HOGENOM" id="CLU_127424_0_0_1"/>
    <dbReference type="InParanoid" id="W5LZQ0"/>
    <dbReference type="OMA" id="TEFFHYA"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG11"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001410">
        <property type="expression patterns" value="Expressed in bone element and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF89">
        <property type="entry name" value="CKLF-LIKE MARVEL TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="34"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="170" mass="18666" checksum="CEC6E0F3A0DEBC6E" modified="2014-04-16" version="1">STLSFSKMSHTIVTATTTSTTSSSSDGGAIYFGYARSLPGILKIAQVLVLLVAFLCVHLCSNWTNYDAYRYFEVVTVWFLIVFLIFFIMHIFRLQSKLPCINWTFTEFLHYAIGTILIFIASIVAAVKSHGVSCLVAGSVFGFIATFLLAVTLWMSYKVTCGSQPTGAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0C3</accession>
    <name>W5M0C3_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guided entry of tail-anchored proteins factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tail-anchored protein insertion receptor WRB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophan-rich basic protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WRB/GET1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01003344">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01003345">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0C3"/>
    <dbReference type="FunCoup" id="W5M0C3">
        <property type="interactions" value="686"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001830"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001835.1">
        <property type="protein sequence ID" value="ENSLOCP00000001830.1"/>
        <property type="gene ID" value="ENSLOCG00000001591.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008610"/>
    <dbReference type="HOGENOM" id="CLU_121992_0_0_1"/>
    <dbReference type="InParanoid" id="W5M0C3"/>
    <dbReference type="OMA" id="AEWIISF"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG3"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001591">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000004">
        <property type="entry name" value="tail-anchored protein insertion receptor WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="181" mass="20466" checksum="E1ACCCDC0705E346" modified="2014-04-16" version="1">RARERAPRGVKMADGCAWILVLGSVLLCNLVKALLPTVSSLLSRILQKDAEQETQMRMEIQDMRQELSAISMMDEFARYARLERRINKMTDKLKTHVKSRTAQLAKIKWVVNIVFYILQAALMISLIWKYYADPVMVLPSKWIAPLERLVAFPSGMVGGVGITCWLVVCNKVVAIVLQAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0H6</accession>
    <name>W5M0H6_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sad1 and UNC84 domain containing 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus inner membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AHAT01032348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0H6"/>
    <dbReference type="FunCoup" id="W5M0H6">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000001883"/>
    <dbReference type="Ensembl" id="ENSLOCT00000001888.1">
        <property type="protein sequence ID" value="ENSLOCP00000001883.1"/>
        <property type="gene ID" value="ENSLOCG00000001625.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155830"/>
    <dbReference type="InParanoid" id="W5M0H6"/>
    <dbReference type="OMA" id="IYTPPQC"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG13"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001625">
        <property type="expression patterns" value="Expressed in camera-type eye and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000009">
        <property type="entry name" value="SUN domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032680">
        <property type="entry name" value="SUN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040994">
        <property type="entry name" value="Sun_CC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF23">
        <property type="entry name" value="SUN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18580">
        <property type="entry name" value="HTH_SUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09387">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="8">
        <location>
            <begin position="816"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000001883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="979" mass="110441" checksum="12FCA54297C08BD5" modified="2014-04-16" version="1">MDFSRLHIYTPPQCVPENTGYTYSLSSSYSSAALDFESQHKLEPVFDSPRMSRRSLRLAITGHFSDDGFLDSSRSKVSYSGGGRAYRTLNRTSHKQTALTSQQTPSKTQSDSSLILGQNSFHSCASDVSLMSTLLDESSIQEKTLVDSFWKERERRASSKSCFSGNGDLNTAETQTSMANGYICNDCSILSQRKDVLTTYSSSSHSHSPTPSSTVYSYDKTKRHKSGILHSIRVAAHSSFCGSMNVKDLMTGDGRLNLNGSLCDDCKGKKHLETQTVHMRSSRFSTATRTLWHIVAYTAGAIFFSYCNKFHKRRNFVWSASSGYNFISWCSCLSSTEIISVNETSSFSFKFSKLKFVIFSYADYYLLFVGYYLFQAVRTVASAGWFVTKKLWSFLWLAVVSPGRAATGAFWWLGTGWYHLVTLMSLLNVFILTRCLPKLLKLLLLLIPFLLLLGLWYWSPSGLLLSLPGLISSDSLFNKCMHSFSIFTSLLTDVSFKQKLHLTMLAEESMHKKRHLLPLFVVQSVSGVFDLERVISLEKRLALLSDSCQKTSQQYDERHRQLLGLYGKLQEQLARARDEEGASTRITNLFDQRMSILKQDLQQSWEEKEQNQNEYLALHQSHESRIADLEGLLQIIAAKTEEVRQKRETSTPAAVSQESDDHSLLLSEVKRLELELNQIKADLLGVKTCHSKCEALDSLQDTVDARVREAVKTLIYGSREAEMPESLLQWLSSQYVQHGDLQGRLASLEQRVFKNITSYTSQTKQMPTAQVVTSAVNGAGVTGITEEQVHAIVNNALRLYSQDRTGLVDYALESGGGSILSTRCSETYETKTALMSLFGVPLWYFSQSPRVVIQPDVYPGNCWAFKGSQGYLVIRLSLTIIPSSFTLEHIPKALSPTGHISSAPRNFTVYGLEDEYQEEGKLLGRYTYEEDGESFQTFPVTEENDKAYQIIEVRVLSNWGHPEYTCLYRFRVHGEPYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M0Y9</accession>
    <name>W5M0Y9_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Carnitine palmitoyltransferase 1Aa (liver)</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01008148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01008151">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M0Y9"/>
    <dbReference type="FunCoup" id="W5M0Y9">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002046"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002051.1">
        <property type="protein sequence ID" value="ENSLOCP00000002046.1"/>
        <property type="gene ID" value="ENSLOCG00000001740.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286917"/>
    <dbReference type="HOGENOM" id="CLU_013513_2_1_1"/>
    <dbReference type="InParanoid" id="W5M0Y9"/>
    <dbReference type="OMA" id="AWIQMAL"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG27"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001740">
        <property type="expression patterns" value="Expressed in larva and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF55">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, BRAIN ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="7">
        <location>
            <begin position="85"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="696" mass="80022" checksum="282E405F174562CF" modified="2025-10-08" version="2">MSPCSKYLTDGNQRIVGGVLVGTGLWVALIYTMRSALKTLLSWHGWMYGQHGTLSRTTRFWMLFVKLFSGRRPMLYSFQTSLPRLPVPRVKDTMRRVLMSIRPLMDDEEYKRMEALAKDFEANLGPRLQWYLNLKSWWATNYVSDWWEEYIYLRGRGPIMVNSNYYAMDFLYVTPTRLQAARAGNTIHAIMLYRRKLDREEIKPLMVQNTIPMCSSQYERMFNTSRIPGVDTDTLQHMRDSKHIVVYHKGRFFKVWMYHDGRLLKPREIEQQMERILADKSEPQPGEEKLAALTAGDRVPWASARQAYFSKGKNKQALDTVEKAAFFMTLDDSEQGFVKESPVDSLSNYAKSLLHGQCYDRWFDKSFTFIVYKNGTMGLNAEHSWADAPIVGHLWEQVLAIDSFQLGYTEDGHCKGELNSSIPGPQRLQWDITEECQGVIDSSFTVAKALSDDVDIHIVPFNTFGKGLIKKCKTSPDAFIQIALQLAHFRDKGKFCLTYEASMTRLFREGRTETVRSCTVESSEFVRAMMNSATKEEKIKLFQRAAEKHQHMYRLAMTGKGIDRHLFCLYVVSKYLGVDSPFLKQVLSEPWRLSTSQTPHQQLELFDLRNNPNHVTSGGGFGPVADDGYGVSYIIIGENHINFHISSKLSSHETDSHRFGNNITQAMLDILNLFELDNKPVNHALWVSTLTTKPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1B2</accession>
    <name>W5M1B2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 161B</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM161 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01023459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1B2"/>
    <dbReference type="FunCoup" id="W5M1B2">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002169"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002174.1">
        <property type="protein sequence ID" value="ENSLOCP00000002169.1"/>
        <property type="gene ID" value="ENSLOCG00000001873.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000672"/>
    <dbReference type="InParanoid" id="W5M1B2"/>
    <dbReference type="OMA" id="RFALMPI"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG2"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001873">
        <property type="expression patterns" value="Expressed in ovary and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098901">
        <property type="term" value="P:regulation of cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010882">
        <property type="term" value="P:regulation of cardiac muscle contraction by calcium ion signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002027">
        <property type="term" value="P:regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019395">
        <property type="entry name" value="Transmembrane_161A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13624">
        <property type="entry name" value="RE42071P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13624:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 161B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10268">
        <property type="entry name" value="Tmemb_161AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="523" mass="58730" checksum="5A57D5DBEA12B5E9" modified="2014-04-16" version="1">MTAPVPGQQCLQLETEPIILCLGGQSLNHCSILHHTSGIIGVQLVVTMVMASVIQKIIPHYSFARWLLCSGSLRWYQHPTEDELRSLAGKQQKGKNKKDRKYNGHIENKPMTVPKDIDLQLETKSIAEVDTLALHYFPEYQWLVDFTVAATIVYLITEFYYSLMKPSSEMNISVIWCLLVLAFVIKVLFSLTAHYFRVEEGGERSVCITFAFFFFVKAMAILIVTENYLEFGLETGFANFSESAVQFLEKQGLESQGPISKLTFKLFLALLCSVIGAFLTFPGLRLAQMHLDALNLATAKVTQTLLHINFLAPLIMVLLWVKPITKDYIMNPTLGKESVPLMTETTYDTLRLWVIILLCVLRLAMIRHHLQAYLNLAQKCVDQMKKEAGRISTVELQKMVARVFYYLCVIALQYVAPLVMLLHTTLLLKTLGGHSWGVYSEPEVPSTLLTGLDPAAAASEPLAPPEKAKVSMAQITVALGGLRNVFTPLLFRGLLSFLTWWIAACLFSTSLFGLFYHQYLTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5M1L1</accession>
    <name>W5M1L1_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bri3 binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01028701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01028702">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M1L1"/>
    <dbReference type="FunCoup" id="W5M1L1">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002269"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002274.1">
        <property type="protein sequence ID" value="ENSLOCP00000002269.1"/>
        <property type="gene ID" value="ENSLOCG00000001962.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="ENOG502QRNV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002024"/>
    <dbReference type="HOGENOM" id="CLU_095006_0_0_1"/>
    <dbReference type="InParanoid" id="W5M1L1"/>
    <dbReference type="OMA" id="LMDTFWR"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000001962">
        <property type="expression patterns" value="Expressed in camera-type eye and 12 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033367">
        <property type="entry name" value="BRI3BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31253">
        <property type="entry name" value="BRI3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31253:SF0">
        <property type="entry name" value="BRI3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14965">
        <property type="entry name" value="BRI3BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="229" mass="26281" checksum="0D0E3FA7693B4AF5" modified="2014-04-16" version="1">RRRYNTQIQEKNHLRCVEAARSRKDSSSQNSFRRAANGLYQTLSSIFGEDNIRALHKFFSKTTERFVYGVDAFLDTIWKIWTDLLDVLGIDSSNLTYYFSQTALTSSPARTLLLVASVVVAYWLLSLFLGGLFYLLQVAFGRFFWLVRVVLFALSCLYILQKFEGDPERAVLPLCFVMAVYFMTGPVGSYWRKGGSSLEDKIDHLDGQIRLLNIRLSRVIESLDRGSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2I8</accession>
    <name>W5M2I8_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA pseudouridine synthase</fullName>
            <ecNumber evidence="7">5.4.99.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="7"/>
    <dbReference type="EMBL" id="AHAT01034606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034607">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01034610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2I8"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002602.1">
        <property type="protein sequence ID" value="ENSLOCP00000002596.1"/>
        <property type="gene ID" value="ENSLOCG00000002210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="102693982"/>
    <dbReference type="KEGG" id="loc:102693982"/>
    <dbReference type="CTD" id="126789"/>
    <dbReference type="GeneTree" id="ENSGT00950000183160"/>
    <dbReference type="HOGENOM" id="CLU_014673_3_1_1"/>
    <dbReference type="OrthoDB" id="271910at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG25"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002210">
        <property type="expression patterns" value="Expressed in pharyngeal gill and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000011">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000006">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="12"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="162"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="295" mass="33801" checksum="57EFCB6F870EADA5" modified="2014-04-16" version="1">MMSCKVRYLIFFQYFGSKYSGVMRSPAHQAVLGVQNYLEDAVRKLNPVNEVSVYVSSRTDSGVHALCNTAHVDIQRKENKPPFSEQILTEALNYHLKPEPISIMKSSIVPPLFHARFNAKSRTYIYRLVTGLRNSSQLPVFEKDLCWLVQEDQLNVEAMQEAARLFLGTHNFSTFRALNSETPYKSPVKTMLLADITPGYSYFAQHHLNRNMQYWDLTFKSNSFLYKQVRRMTGALVAVGQGRLTLPRLQELLEVQDSLAFPRNMSAPPQGLFLKEVEYDDSELLPRPLDPDIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2M5</accession>
    <name>W5M2M5_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uroplakin-3b-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01005643">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015222872.1">
        <property type="nucleotide sequence ID" value="XM_015367386.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2M5"/>
    <dbReference type="FunCoup" id="W5M2M5">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000002633"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002639.1">
        <property type="protein sequence ID" value="ENSLOCP00000002633.1"/>
        <property type="gene ID" value="ENSLOCG00000002256.1"/>
    </dbReference>
    <dbReference type="GeneID" id="107075324"/>
    <dbReference type="KEGG" id="loc:107075324"/>
    <dbReference type="eggNOG" id="ENOG502RZJD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153392"/>
    <dbReference type="InParanoid" id="W5M2M5"/>
    <dbReference type="OMA" id="YKTHYSS"/>
    <dbReference type="OrthoDB" id="9945328at2759"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG22"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002256">
        <property type="expression patterns" value="Expressed in zone of skin and 5 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024831">
        <property type="entry name" value="Uroplakin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446">
        <property type="entry name" value="UROPLAKIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446:SF2">
        <property type="entry name" value="UROPLAKIN-3B-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004865563" evidence="2">
        <location>
            <begin position="20"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="272" mass="29309" checksum="8593958CE168D839" modified="2014-04-16" version="1">MSSVLVVTFMSVWFRVAKGLSTVPYAPEVTPHNLLGRVTSTTLTLQQPVCFFNNLSGLPCAPDNCEIWLVIARGDGVPKFEAIKGNTSVLMSSPYPDAFRNNSSPNYFLTKLGVQNAFPCAESTGFRFFRVGDEGLCSTSNCNGILPAGSTVRAKYVLLDPGSKQVVSESQWSSPISLIALSDSAGIDEWIGKRSGGMIVVTSILSSLLAILLLFLLTAFVLRCTGFQKVKQDVTMPPALGSVRVKKYSTHNVRNAEAVHSPGVTHTVTTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5M2W2</accession>
    <name>W5M2W2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Proprotein convertase subtilisin/kexin type 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01011218">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AHAT01011219">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M2W2"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000002720"/>
    <dbReference type="Ensembl" id="ENSLOCT00000002726.1">
        <property type="protein sequence ID" value="ENSLOCP00000002720.1"/>
        <property type="gene ID" value="ENSLOCG00000002313.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161989"/>
    <dbReference type="HOGENOM" id="CLU_002976_4_0_1"/>
    <dbReference type="InParanoid" id="W5M2W2"/>
    <dbReference type="OMA" id="RDGTCQE"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG19"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002313">
        <property type="expression patterns" value="Expressed in testis and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000034">
        <property type="entry name" value="furin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF3">
        <property type="entry name" value="FURIN-LIKE PROTEASE 1, ISOFORMS 1_1-X_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="P/Homo B" evidence="15">
        <location>
            <begin position="434"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000002720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="675" mass="74297" checksum="8233FED6DBF42FED" modified="2014-04-16" version="1">SIVLISTVCLAEGSYTSTWAVRVQGPPEDADKIARKYGFVNFGKVFEDGHYYHMRHGAVAPKSSKQHHLRNLQLKRDPKVLWHEQQSGKVRQRRHAFHLPTDPLFSKQWYLSEAFQLNVVSAWAQGYTGKGVVVSILDDGIEKSHPDLAANYDPEASYDMNDDDPDPQPRYTTDNENRHGTRCAGEVAAVANNTVCGVGVAYRAQIGGRGGLGVRMLDGHVTDLVEALSLSWKSQHIDIYSASWGPQDDGRSLDGPGVLAKEALIRGVIQGRRGLGSIFVWASGNGGAHFDNCNCDGYANSMYTVLVGSTTERGTVPEYSEPCSAILTTTYSSGSKLHRKIVTTDLRATCTAEHSGTSASAPLAAGIIALALEANPTLTWRDVQHVVIRASRPAGLRADDWQLNGVGRAVSHHYGYGLLDAGKLVNLARKWKTVRPQRKCFIDVVTRPMEIRGQLPFRWNVSACSGTGDWIRSLEHVQARLSLACSRRGDLAISLTSPRGTRSLLATARPRDTSPQGYSDWAFTTTHCWDEDPRGVWTLSLEDKGDGTNTGVLSHFRLELYGTEENVAERGVERTVVEECLDWSPQGICQECRYPLYAFDRVCLVFCPPHHYEWSLNGSGMGVAEARRCQPCHPSCYTCHGEGERSCASCPPLSTHNALLGTCSPVIYAWDQEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5M3P6</accession>
    <name>W5M3P6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ran-specific GTPase-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ran-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L. Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN. Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1. Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1. Identified in a complex with nucleotide-free RAN and RCC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RANBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01028603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M3P6"/>
    <dbReference type="FunCoup" id="W5M3P6">
        <property type="interactions" value="1633"/>
    </dbReference>
    <dbReference type="STRING" id="7918.ENSLOCP00000003004"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003010.1">
        <property type="protein sequence ID" value="ENSLOCP00000003004.1"/>
        <property type="gene ID" value="ENSLOCG00000002540.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154389"/>
    <dbReference type="InParanoid" id="W5M3P6"/>
    <dbReference type="OMA" id="NFKDSFM"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG20"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002540">
        <property type="expression patterns" value="Expressed in embryo and 13 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046604">
        <property type="term" value="P:positive regulation of mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000824">
        <property type="entry name" value="Ran-specific GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF94">
        <property type="entry name" value="RAN BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="10">
        <location>
            <begin position="26"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="233" mass="27137" checksum="24FE0E3E1FC82C9C" modified="2014-04-16" version="1">MADTKDTQEDHETTAENADESTHDPHFEPIVSLPEQEVKTLEEDEEELFKMRAKLYRFASENDPPEWKERGTGDVKLLRHKEKGTIRLLMRRDRTLKICANHNIAPLMELRPNAGSDRAWVWNTHADFADEEPKPELLAIRFLNAENAQKFKAKFDECKEEVIRSQEISGNDDSTNKVAEKLEELSVKDVVTKDSAGQNDEEQPKKKEEEEPEKKAESFGFHCFFFCFFYDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5M477</accession>
    <name>W5M477_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium- and chloride-dependent glycine transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Solute carrier family 6 member 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>glycine(out) + chloride(out) + 2 Na(+)(out) = glycine(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70691"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A9 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AHAT01018191">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5M477"/>
    <dbReference type="STRING" id="7918.ENSLOCP00000003185"/>
    <dbReference type="Ensembl" id="ENSLOCT00000003192.1">
        <property type="protein sequence ID" value="ENSLOCP00000003185.1"/>
        <property type="gene ID" value="ENSLOCG00000002674.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157263"/>
    <dbReference type="InParanoid" id="W5M477"/>
    <dbReference type="Proteomes" id="UP000018468">
        <property type="component" value="Linkage group LG10"/>
    </dbReference>
    <dbReference type="Bgee" id="ENSLOCG00000002674">
        <property type="expression patterns" value="Expressed in camera-type eye and 11 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903804">
        <property type="term" value="P:glycine import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060080">
        <property type="term" value="P:inhibitory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060092">
        <property type="term" value="P:regulation of synaptic transmission, glycinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003028">
        <property type="entry name" value="Na/ntran_symport_glycine_GLY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF263">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01204">
        <property type="entry name" value="GLY1TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="604"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="634"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="650" mass="72773" checksum="60B1885316CF6832" modified="2014-04-16" version="1">VIIRLEFNKYSGSEQNGAVPGEAVKKDENTRRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGVPGAFMFPYFIMLVFCGIPLFFMELSFGQFASLGCLGVWKVSPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFMSMTSLLPWTYCNNQWNTKDCSGVVGTSYANATALMSNFTELANRTVKRTSPSEEYWKYYVLNISDDIGNFGEVRLPILGCLAVSWVVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFIRGITLDGAINGIKYYLTPQWQKILDAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCFRYRDSIIISVTNCATSVYAGFVIFSILGFMAHNLGVNVADVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEIGTDWIIRNKTYVTLGVAILGFLLGVPLTTQAGIYWLLLMDNYAASFSLVVISCIMCVCIMYVYGHVNYFKDVEMMLGFPPPVFFRICWRFISPVIITFILVFTVIQYKPITYNNYIYPGWSLVIGFLMALSSVICIPIYARKLRQLLGIEGRISKQRLKNAVKPHHTWGPALQEHRIGRYAPTVSPSADRGVEAPLHEKEKETETEKKPEEISLTIQGSNGSTHNPDTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4D2</accession>
    <name>W5M4D2_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">THAP domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AHAT01009677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSLOCP00000003240.1"/>
        <property type="gene ID" value="ENSLOCG00000002750.1"/>
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    <dbReference type="eggNOG" id="ENOG502S14P">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Linkage group LG25"/>
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        <property type="entry name" value="THAP3"/>
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        <property type="entry name" value="THAP_Znf"/>
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    <dbReference type="PANTHER" id="PTHR47120">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47120:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 3"/>
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        <property type="entry name" value="DM3"/>
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        <location>
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            <begin position="126"/>
            <end position="135"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="235" mass="27266" checksum="1AC9B86F050C380B" modified="2014-04-16" version="1">MPKSCSAYNCTNRYSSKNPDLTFHRFPFSKPHVLKEWLDNIARDDFQPKRHMVICSQHFTPDCFSSFSNRKNLLWNAVPTLFSFPPTGSKRKKSRKKLKTERVAAASKWEELWLEEVGPQQEEVELDCRGEREEPVTTETAESLADRSPESTPQVNFEVPVSRLSQEPWPPDHSYALAEPGEVKTRLFSALEANERLRGRLKVKCRRLRQTSARLRAARRQLGRLRTRLGRARAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5M4R6</accession>
    <name>W5M4R6_LEPOC</name>
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        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSLOCT00000003381.1">
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        <property type="gene ID" value="ENSLOCG00000002865.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0029">
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF342">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004867619" description="Amine oxidase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="13">
        <location>
            <begin position="67"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSLOCP00000003374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018468"/>
        </source>
    </evidence>
    <sequence length="522" mass="58495" checksum="2E5A73FCDCDE1CF1" modified="2014-04-16" version="1">MRPGTAVSPALTLLGCFLALLAQGQAYSSAIQRCFHDKDSEFLLDIAKHGLIRKGPAKKFLIVGAGISGLIAAKLLEDVGHDVQILEASDRVGGRILTHRNKTRGWYAELGAMRIPSTHELVHHYVQHYGLKLSEFIQADDSAWYFTNGGLHRVFDINKNPDLLGYALEKSEKGRSPEQLYSEAIQKVIDEIERDPSQCQCILKKYDVYSVKEYLKRESNLSRHAIQMIGDILNSQSLFYTAFSENLRDEADISDDVKFYKIDGGTDLLPQALRQDLRRSRVQLESRVLEINQTGEGVRVAYRHKPDNAVQTAAADYLLFTGTAKATLNVRFNPSLPLQKREALRSAHYDSATKIFLVFKERFWEKEGIRGGKSVTDRPSRFIYYGSEAFESGVGLVLASYTWADESLFFLGMSDEACQEAALSDLAALHGEGVRALYEGGVVKKWSLDEYSLGAFALFTPFQLTDYHRDLSAPSQRVYFAGEHTALPHAWIETSVKSALRAARRMNHDAAAKAVHHLKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5H3</accession>
    <name>W5M5H3_LEPOC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ARF guanine nucleotide exchange factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
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        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="667"/>
            <end position="856"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <begin position="1525"/>
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        </location>
    </feature>
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            <begin position="1"/>
            <end position="18"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="248"/>
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        </location>
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            <end position="358"/>
        </location>
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            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="641"/>
            <end position="654"/>
        </location>
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            <begin position="1529"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
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            <begin position="1540"/>
            <end position="1561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
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    </evidence>
    <sequence length="1826" mass="205668" checksum="63D214B152BABFCC" modified="2014-04-16" version="1">QWRSEARKAMQQKEESSKTKSMFLSRALEKILSDKEVKRAQHSQLRKACQVALDEIKAELEKQRDGSVVPPKANYIEADKYVLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETVCNCFQGPQTDEGVQLQIIKALLTTVTSPHIEIHEGTILMTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMESQATLETRELEKARHWQHQARQSPVPAAPAGSPRLDAPVHPEHRQPPEPQSEPAPDAQLPAGGSSEHLNGEPVEGSPGTGEAQDRTSDPSQENAEVPKTEPHASAEPAGEVQESDPVPGKDAEEPLGDSEQQVSESKDLRPPSDRTETAQTNGIVDDRASVSSTDILDTDAAHGPPTARFSHILQKDAFLVFRSLCKLSMKPLADGPPDPRSHELRSKVVSLQLLLSVLQSAGPVFRTHEMFVNAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKIHLKMQIEVFFREIFLTILETSSSSFEHKWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGQELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNLQTNLGQEKPPEQESGESRPLESCTRRDSVSSMDSTVSSGIGSASTQASLADDPEQFEVMKQQKEIIEHGIELFNKKPKRGIQYLQEQGMLGTLPEDIAQFLHQEERLDTTQVGEFLGENIRFNKEVMYSYVDQLDFCGKDFVSALRIFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEENLSAIYDEIAGKKIAMKESKEYSITPKSNKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAQAPFTSATHLEHVRPMFKLAWTPLLAAFSIGLQDCDDQEVASLCLEGIRCAVRIASIFGMQLERDAYVQALARFTLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILRCISQLELAQLIGTGVKTRYISGMVREREASIKGFPSGSEEFMPLGLGNLVGGPDKRQMASIQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVHWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWQVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVNSQAANIRSGWKNIFSVFHQAASDHDETIVELAFQTTGHIVVSTYQKHFAAAIDSFQDAVKCLSEFVCNAAFPDTSMEAIRLIRHCAKYVSERPQALREYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWHDLFRIVFRIFDNMKLPEQQTEKTEWMTTTCNHALYAICDVFTQFYDPLSDVLLPDIFTQIQWCVSQDNEQLARSGTNCLENLVILNGEKFSPEVWEHTCLCMLDIFHNTSPHVLLTWRPASQEEDTGEKHMDVEVDSHSQSSYERNLSDRGQSQMSNASDETWKGKSYAKIADQKLFAWLLIKCVVQLELIQTIDNIVFYPATSKKEDADNMTAAQRDALDEGAPEEVGSQGMYGYMSSLHLFKLLDCLLESHSFAKAFNSNNEQRTALWRAAGFKGKSKPNLLKQETSSLACCLRILFRMYMDDKLKDSWSAIQARLLRVCSEALAYFITLTSESHREAWTSLLLLLLTKTLKLTDDKFRDHASCYYPYLCEIMQFDLIPELRVVLRRFFLRIGSVFKISSAEGQLRLVGDTEPGRVAPAAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5M5U6</accession>
    <name>W5M5U6_LEPOC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D(3) dopamine receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Dopamine D3 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Lepisosteus oculatus</name>
        <name type="common">Spotted gar</name>
        <dbReference type="NCBI Taxonomy" id="7918"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Holostei</taxon>
            <taxon>Semionotiformes</taxon>
            <taxon>Lepisosteidae</taxon>
            <taxon>Lepisosteus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-12" db="EMBL/GenBank/DDBJ databases">
            <title>The Draft Genome of Lepisosteus oculatus.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Assembly &amp; Analysis Group"/>
                <consortium name="Computational R&amp;D Group"/>
                <consortium name="and Sequencing Platform"/>
                <person name="Di Palma F."/>
                <person name="Alfoldi J."/>
                <person name="Johnson J."/>
                <person name="Berlin A."/>
                <person name="Gnerre S."/>
                <person name="Jaffe D."/>
                <person name="MacCallum I."/>
                <person name="Young S."/>
                <person name="Walker B.J."/>
                <person name="Lander E.S."/>
                <person name="Lindblad-Toh K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
           
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